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PMC:1420271 / 15229-16392 JSONTXT

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craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T8165 38-43 NCBITaxon:10088 denotes mouse
T8166 44-48 PR:000006847 denotes ESG1
T8167 49-53 SO:0000704 denotes gene
T8168 62-80 SO:0001417 denotes 3' flanking region
T8169 102-104 SO:0000028 denotes bp
T8170 141-147 SO:0000440 denotes vector
T8171 162-169 PR:P43870 denotes HindIII
T8172 207-213 SO:0000440 denotes vector
T8173 286-290 PR:000006847 denotes ESG1
T8174 291-301 SO:0000336 denotes pseudogene
T8175 310-328 SO:0001417 denotes 3' flanking region
T8176 336-338 SO:0000028 denotes bp
T8177 393-398 PR:P23940 denotes BamHI
T8178 427-433 SO:0000440 denotes vector
T8179 515-534 SO:0001416 denotes 5' flanking regions
T8180 542-546 PR:000006847 denotes ESG1
T8181 547-551 SO:0000704 denotes gene
T8182 568-579 SO:0000336 denotes pseudogenes
T8183 629-633 PR:000006847 denotes pH34
T8184 681-685 PR:000006847 denotes pH34
T8185 691-698 SO:0000112 denotes primers
T8186 778-780 SO:0000028 denotes bp
T8187 961-979 SO:0001416 denotes 5' flanking region
T8188 987-991 PR:000006847 denotes ESG1
T8189 992-996 SO:0000704 denotes gene
T8190 1004-1006 SO:0000028 denotes bp
T8191 1139-1147 SO:0000357 denotes flanking
T8192 1152-1162 SO:0000336 denotes pseudogene

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T8228 38-43 NCBITaxon:10088 denotes mouse
T8229 44-48 PR_EXT:000006847 denotes ESG1
T8230 49-53 SO_EXT:0000704 denotes gene
T8231 62-80 SO_EXT:0001417 denotes 3' flanking region
T8232 102-104 SO_EXT:0000028 denotes bp
T8233 141-147 SO_EXT:0000440 denotes vector
T8234 162-169 PR_EXT:P43870 denotes HindIII
T8235 207-213 SO_EXT:0000440 denotes vector
T8236 224-230 SO_EXT:sequence_cloning_process denotes cloned
T8237 286-290 PR_EXT:000006847 denotes ESG1
T8238 291-301 SO_EXT:0000336 denotes pseudogene
T8239 310-328 SO_EXT:0001417 denotes 3' flanking region
T8240 336-338 SO_EXT:0000028 denotes bp
T8241 362-368 SO_EXT:sequence_cloning_process denotes cloned
T8242 393-398 PR_EXT:P23940 denotes BamHI
T8243 427-433 SO_EXT:0000440 denotes vector
T8244 444-450 SO_EXT:sequence_cloning_process denotes cloned
T8245 491-499 SO_EXT:biological_sequence denotes sequence
T8246 515-534 SO_EXT:0001416 denotes 5' flanking regions
T8247 542-546 PR_EXT:000006847 denotes ESG1
T8248 547-551 SO_EXT:0000704 denotes gene
T8249 568-579 SO_EXT:0000336 denotes pseudogenes
T8250 629-633 PR_EXT:000006847 denotes pH34
T8251 681-685 PR_EXT:000006847 denotes pH34
T8252 691-698 SO_EXT:0000112 denotes primers
T8253 714-722 SO_EXT:biological_sequence denotes sequence
T8254 727-733 SO_EXT:sequence_cloning_process denotes cloned
T8255 778-780 SO_EXT:0000028 denotes bp
T8256 887-893 SO_EXT:sequence_cloning_process denotes cloned
T8257 961-979 SO_EXT:0001416 denotes 5' flanking region
T8258 987-991 PR_EXT:000006847 denotes ESG1
T8259 992-996 SO_EXT:0000704 denotes gene
T8260 1004-1006 SO_EXT:0000028 denotes bp
T8261 1062-1068 SO_EXT:sequence_cloning_process denotes cloned
T8262 1139-1147 SO:0000357 denotes flanking
T8263 1152-1162 SO_EXT:0000336 denotes pseudogene

craft-sa-dev

Id Subject Object Predicate Lexical cue
T8438 0-2 TO denotes To
T8439 3-11 VB denotes sequence
T8440 85-94 VBD denotes subcloned
T8441 12-15 DT denotes the
T8442 16-22 NN denotes region
T8443 23-33 VBG denotes containing
T8444 34-37 DT denotes the
T8445 49-53 NN denotes gene
T8446 38-43 NN denotes mouse
T8447 44-48 NN denotes ESG1
T8448 54-57 CC denotes and
T8449 58-61 DT denotes the
T8450 74-80 NN denotes region
T8451 62-63 CD denotes 3
T8452 63-64 SYM denotes '
T8453 65-73 NN denotes flanking
T8454 80-82 , denotes ,
T8455 82-84 PRP denotes we
T8456 95-96 DT denotes a
T8457 115-123 NN denotes fragment
T8458 97-98 SYM denotes ~
T8459 98-100 CD denotes 15
T8460 101-104 NN denotes kbp
T8461 105-109 NN denotes XhoI
T8462 110-114 NN denotes SalI
T8463 109-110 HYPH denotes -
T8464 124-128 IN denotes into
T8465 129-132 DT denotes the
T8466 141-147 NN denotes vector
T8467 133-138 NN denotes pZERO
T8468 138-139 HYPH denotes -
T8469 139-140 CD denotes 2
T8470 148-149 -LRB- denotes (
T8471 149-159 NNP denotes Invitrogen
T8472 159-160 -RRB- denotes )
T8473 160-161 . denotes .
T8474 161-269 sentence denotes HindIII- or EcoRI-digested fragments of this vector were then cloned into pBluescript KS(-) for sequencing.
T8475 162-169 NN denotes HindIII
T8476 180-188 VBN denotes digested
T8477 169-170 HYPH denotes -
T8478 171-173 CC denotes or
T8479 174-179 NN denotes EcoRI
T8480 179-180 HYPH denotes -
T8481 189-198 NNS denotes fragments
T8482 224-230 VBN denotes cloned
T8483 199-201 IN denotes of
T8484 202-206 DT denotes this
T8485 207-213 NN denotes vector
T8486 214-218 VBD denotes were
T8487 219-223 RB denotes then
T8488 231-235 IN denotes into
T8489 236-247 NN denotes pBluescript
T8490 248-250 NN denotes KS
T8491 250-251 -LRB- denotes (
T8492 251-252 SYM denotes -
T8493 252-253 -RRB- denotes )
T8494 254-257 IN denotes for
T8495 258-268 NN denotes sequencing
T8496 268-269 . denotes .
T8497 269-392 sentence denotes To sequence the ESG1 pseudogene and the 3' flanking region, an 8 kbp NotI/XhoI fragment was cloned into pBluescript KS(-).
T8498 270-272 TO denotes To
T8499 273-281 VB denotes sequence
T8500 362-368 VBN denotes cloned
T8501 282-285 DT denotes the
T8502 291-301 NN denotes pseudogene
T8503 286-290 NN denotes ESG1
T8504 302-305 CC denotes and
T8505 306-309 DT denotes the
T8506 322-328 NN denotes region
T8507 310-311 CD denotes 3
T8508 311-312 SYM denotes '
T8509 313-321 NN denotes flanking
T8510 328-330 , denotes ,
T8511 330-332 DT denotes an
T8512 349-357 NN denotes fragment
T8513 333-334 CD denotes 8
T8514 335-338 NN denotes kbp
T8515 339-343 NN denotes NotI
T8516 344-348 NN denotes XhoI
T8517 343-344 HYPH denotes /
T8518 358-361 VBD denotes was
T8519 369-373 IN denotes into
T8520 374-385 NN denotes pBluescript
T8521 386-388 NN denotes KS
T8522 388-389 -LRB- denotes (
T8523 389-390 SYM denotes -
T8524 390-391 -RRB- denotes )
T8525 391-392 . denotes .
T8526 392-474 sentence denotes BamHI- or PstI- fragments of this vector were also cloned into pBluescript KS(-).
T8527 393-398 NN denotes BamHI
T8528 409-418 NNS denotes fragments
T8529 398-399 SYM denotes -
T8530 400-402 CC denotes or
T8531 403-407 NN denotes PstI
T8532 407-408 SYM denotes -
T8533 444-450 VBN denotes cloned
T8534 419-421 IN denotes of
T8535 422-426 DT denotes this
T8536 427-433 NN denotes vector
T8537 434-438 VBD denotes were
T8538 439-443 RB denotes also
T8539 451-455 IN denotes into
T8540 456-467 NN denotes pBluescript
T8541 468-470 NN denotes KS
T8542 470-471 -LRB- denotes (
T8543 471-472 SYM denotes -
T8544 472-473 -RRB- denotes )
T8545 473-474 . denotes .
T8546 474-699 sentence denotes To identify the sequence containing the 5' flanking regions of the ESG1 gene and the related pseudogenes, we used a TOPO walker kit (Invitrogen) with the pH34-T2L (5'-ACTAGTCGCAGCAGGGATCCAGGAATATCT-3') and pH34-L394 primers.
T8547 475-477 TO denotes To
T8548 478-486 VB denotes identify
T8549 584-588 VBD denotes used
T8550 487-490 DT denotes the
T8551 491-499 NN denotes sequence
T8552 500-510 VBG denotes containing
T8553 511-514 DT denotes the
T8554 527-534 NNS denotes regions
T8555 515-516 CD denotes 5
T8556 516-517 SYM denotes '
T8557 518-526 NN denotes flanking
T8558 535-537 IN denotes of
T8559 538-541 DT denotes the
T8560 547-551 NN denotes gene
T8561 542-546 NN denotes ESG1
T8562 552-555 CC denotes and
T8563 556-559 DT denotes the
T8564 568-579 NNS denotes pseudogenes
T8565 560-567 JJ denotes related
T8566 579-581 , denotes ,
T8567 581-583 PRP denotes we
T8568 589-590 DT denotes a
T8569 603-606 NN denotes kit
T8570 591-595 NN denotes TOPO
T8571 596-602 NN denotes walker
T8572 607-608 -LRB- denotes (
T8573 608-618 NNP denotes Invitrogen
T8574 618-619 -RRB- denotes )
T8575 620-624 IN denotes with
T8576 625-628 DT denotes the
T8577 691-698 NNS denotes primers
T8578 629-633 NN denotes pH34
T8579 634-637 NN denotes T2L
T8580 633-634 HYPH denotes -
T8581 638-639 -LRB- denotes (
T8582 673-674 CD denotes 3
T8583 639-640 CD denotes 5
T8584 640-641 SYM denotes '
T8585 641-642 HYPH denotes -
T8586 642-672 NN denotes ACTAGTCGCAGCAGGGATCCAGGAATATCT
T8587 672-673 HYPH denotes -
T8588 674-675 SYM denotes '
T8589 675-676 -RRB- denotes )
T8590 677-680 CC denotes and
T8591 681-685 NN denotes pH34
T8592 686-690 NN denotes L394
T8593 685-686 HYPH denotes -
T8594 698-699 . denotes .
T8595 699-759 sentence denotes The resulting sequence was cloned into pCR2.1 (Invitrogen).
T8596 700-703 DT denotes The
T8597 714-722 NN denotes sequence
T8598 704-713 VBG denotes resulting
T8599 727-733 VBN denotes cloned
T8600 723-726 VBD denotes was
T8601 734-738 IN denotes into
T8602 739-745 NN denotes pCR2.1
T8603 746-747 -LRB- denotes (
T8604 747-757 NNP denotes Invitrogen
T8605 757-758 -RRB- denotes )
T8606 758-759 . denotes .
T8607 759-932 sentence denotes We obtained a ~6 kbp band from the NsiI-digensted library; XbaI-, SpeI-, EcoRI-, and PstI-digested fragments of this band were cloned into pBluescript KS(-) for sequencing.
T8608 760-762 PRP denotes We
T8609 763-771 VBD denotes obtained
T8610 887-893 VBN denotes cloned
T8611 772-773 DT denotes a
T8612 781-785 NN denotes band
T8613 774-775 SYM denotes ~
T8614 775-776 CD denotes 6
T8615 777-780 NN denotes kbp
T8616 786-790 IN denotes from
T8617 791-794 DT denotes the
T8618 810-817 NN denotes library
T8619 795-799 NN denotes NsiI
T8620 800-809 VBN denotes digensted
T8621 799-800 HYPH denotes -
T8622 817-818 : denotes ;
T8623 819-823 NN denotes XbaI
T8624 850-858 VBN denotes digested
T8625 823-824 HYPH denotes -
T8626 824-826 , denotes ,
T8627 826-830 NN denotes SpeI
T8628 830-831 HYPH denotes -
T8629 831-833 , denotes ,
T8630 833-838 NN denotes EcoRI
T8631 838-839 HYPH denotes -
T8632 839-841 , denotes ,
T8633 841-844 CC denotes and
T8634 845-849 NN denotes PstI
T8635 849-850 HYPH denotes -
T8636 859-868 NNS denotes fragments
T8637 869-871 IN denotes of
T8638 872-876 DT denotes this
T8639 877-881 NN denotes band
T8640 882-886 VBD denotes were
T8641 894-898 IN denotes into
T8642 899-910 NN denotes pBluescript
T8643 911-913 NN denotes KS
T8644 913-914 -LRB- denotes (
T8645 914-915 SYM denotes -
T8646 915-916 -RRB- denotes )
T8647 917-920 IN denotes for
T8648 921-931 NN denotes sequencing
T8649 931-932 . denotes .
T8650 932-997 sentence denotes This fragment contained the 5' flanking region of the ESG1 gene.
T8651 933-937 DT denotes This
T8652 938-946 NN denotes fragment
T8653 947-956 VBD denotes contained
T8654 957-960 DT denotes the
T8655 973-979 NN denotes region
T8656 961-962 CD denotes 5
T8657 962-963 SYM denotes '
T8658 964-972 NN denotes flanking
T8659 980-982 IN denotes of
T8660 983-986 DT denotes the
T8661 992-996 NN denotes gene
T8662 987-991 NN denotes ESG1
T8663 996-997 . denotes .
T8664 997-1096 sentence denotes A ~3 kbp fragment, obtained from the SacI-digested library, was cloned into pCR2.1 for sequencing.
T8665 998-999 DT denotes A
T8666 1007-1015 NN denotes fragment
T8667 1000-1001 SYM denotes ~
T8668 1001-1002 CD denotes 3
T8669 1003-1006 NN denotes kbp
T8670 1062-1068 VBN denotes cloned
T8671 1015-1017 , denotes ,
T8672 1017-1025 VBN denotes obtained
T8673 1026-1030 IN denotes from
T8674 1031-1034 DT denotes the
T8675 1049-1056 NN denotes library
T8676 1035-1039 NN denotes SacI
T8677 1040-1048 VBN denotes digested
T8678 1039-1040 HYPH denotes -
T8679 1056-1058 , denotes ,
T8680 1058-1061 VBD denotes was
T8681 1069-1073 IN denotes into
T8682 1074-1080 NN denotes pCR2.1
T8683 1081-1084 IN denotes for
T8684 1085-1095 NN denotes sequencing
T8685 1095-1096 . denotes .
T8686 1096-1163 sentence denotes This fragment was contained the 5' region flanking the pseudogene.
T8687 1097-1101 DT denotes This
T8688 1102-1110 NN denotes fragment
T8689 1115-1124 VBN denotes contained
T8690 1111-1114 VBD denotes was
T8691 1125-1128 DT denotes the
T8692 1132-1138 NN denotes region
T8693 1129-1130 CD denotes 5
T8694 1130-1131 SYM denotes '
T8695 1139-1147 VBG denotes flanking
T8696 1148-1151 DT denotes the
T8697 1152-1162 NN denotes pseudogene
T8698 1162-1163 . denotes .
R2219 T8438 T8439 aux To,sequence
R2220 T8439 T8440 advcl sequence,subcloned
R2221 T8441 T8442 det the,region
R2222 T8442 T8439 dobj region,sequence
R2223 T8443 T8442 acl containing,region
R2224 T8444 T8445 det the,gene
R2225 T8445 T8443 dobj gene,containing
R2226 T8446 T8445 compound mouse,gene
R2227 T8447 T8445 compound ESG1,gene
R2228 T8448 T8445 cc and,gene
R2229 T8449 T8450 det the,region
R2230 T8450 T8445 conj region,gene
R2231 T8451 T8450 nummod 3,region
R2232 T8452 T8451 punct ',3
R2233 T8453 T8450 compound flanking,region
R2234 T8454 T8440 punct ", ",subcloned
R2235 T8455 T8440 nsubj we,subcloned
R2236 T8456 T8457 det a,fragment
R2237 T8457 T8440 dobj fragment,subcloned
R2238 T8458 T8459 punct ~,15
R2239 T8459 T8460 nummod 15,kbp
R2240 T8460 T8457 compound kbp,fragment
R2241 T8461 T8462 compound XhoI,SalI
R2242 T8462 T8457 compound SalI,fragment
R2243 T8463 T8462 punct -,SalI
R2244 T8464 T8440 prep into,subcloned
R2245 T8465 T8466 det the,vector
R2246 T8466 T8464 pobj vector,into
R2247 T8467 T8466 nmod pZERO,vector
R2248 T8468 T8467 punct -,pZERO
R2249 T8469 T8467 nummod 2,pZERO
R2250 T8470 T8471 punct (,Invitrogen
R2251 T8471 T8466 parataxis Invitrogen,vector
R2252 T8472 T8471 punct ),Invitrogen
R2253 T8473 T8440 punct .,subcloned
R2254 T8475 T8476 npadvmod HindIII,digested
R2255 T8476 T8481 amod digested,fragments
R2256 T8477 T8475 punct -,HindIII
R2257 T8478 T8475 cc or,HindIII
R2258 T8479 T8475 conj EcoRI,HindIII
R2259 T8480 T8476 punct -,digested
R2260 T8481 T8482 nsubjpass fragments,cloned
R2261 T8483 T8481 prep of,fragments
R2262 T8484 T8485 det this,vector
R2263 T8485 T8483 pobj vector,of
R2264 T8486 T8482 auxpass were,cloned
R2265 T8487 T8482 advmod then,cloned
R2266 T8488 T8482 prep into,cloned
R2267 T8489 T8490 compound pBluescript,KS
R2268 T8490 T8488 pobj KS,into
R2269 T8491 T8490 punct (,KS
R2270 T8492 T8490 punct -,KS
R2271 T8493 T8490 punct ),KS
R2272 T8494 T8482 prep for,cloned
R2273 T8495 T8494 pobj sequencing,for
R2274 T8496 T8482 punct .,cloned
R2275 T8498 T8499 aux To,sequence
R2276 T8499 T8500 advcl sequence,cloned
R2277 T8501 T8502 det the,pseudogene
R2278 T8502 T8499 dobj pseudogene,sequence
R2279 T8503 T8502 compound ESG1,pseudogene
R2280 T8504 T8502 cc and,pseudogene
R2281 T8505 T8506 det the,region
R2282 T8506 T8502 conj region,pseudogene
R2283 T8507 T8506 nummod 3,region
R2284 T8508 T8507 punct ',3
R2285 T8509 T8506 compound flanking,region
R2286 T8510 T8500 punct ", ",cloned
R2287 T8511 T8512 det an,fragment
R2288 T8512 T8500 nsubjpass fragment,cloned
R2289 T8513 T8514 nummod 8,kbp
R2290 T8514 T8512 compound kbp,fragment
R2291 T8515 T8516 compound NotI,XhoI
R2292 T8516 T8512 compound XhoI,fragment
R2293 T8517 T8516 punct /,XhoI
R2294 T8518 T8500 auxpass was,cloned
R2295 T8519 T8500 prep into,cloned
R2296 T8520 T8521 compound pBluescript,KS
R2297 T8521 T8519 pobj KS,into
R2298 T8522 T8521 punct (,KS
R2299 T8523 T8521 punct -,KS
R2300 T8524 T8521 punct ),KS
R2301 T8525 T8500 punct .,cloned
R2302 T8527 T8528 nmod BamHI,fragments
R2303 T8528 T8533 nsubjpass fragments,cloned
R2304 T8529 T8527 punct -,BamHI
R2305 T8530 T8527 cc or,BamHI
R2306 T8531 T8527 conj PstI,BamHI
R2307 T8532 T8528 punct -,fragments
R2308 T8534 T8528 prep of,fragments
R2309 T8535 T8536 det this,vector
R2310 T8536 T8534 pobj vector,of
R2311 T8537 T8533 auxpass were,cloned
R2312 T8538 T8533 advmod also,cloned
R2313 T8539 T8533 prep into,cloned
R2314 T8540 T8541 compound pBluescript,KS
R2315 T8541 T8539 pobj KS,into
R2316 T8542 T8541 punct (,KS
R2317 T8543 T8541 punct -,KS
R2318 T8544 T8541 punct ),KS
R2319 T8545 T8533 punct .,cloned
R2320 T8547 T8548 aux To,identify
R2321 T8548 T8549 advcl identify,used
R2322 T8550 T8551 det the,sequence
R2323 T8551 T8548 dobj sequence,identify
R2324 T8552 T8551 acl containing,sequence
R2325 T8553 T8554 det the,regions
R2326 T8554 T8552 dobj regions,containing
R2327 T8555 T8554 nummod 5,regions
R2328 T8556 T8555 punct ',5
R2329 T8557 T8554 compound flanking,regions
R2330 T8558 T8554 prep of,regions
R2331 T8559 T8560 det the,gene
R2332 T8560 T8558 pobj gene,of
R2333 T8561 T8560 compound ESG1,gene
R2334 T8562 T8560 cc and,gene
R2335 T8563 T8564 det the,pseudogenes
R2336 T8564 T8560 conj pseudogenes,gene
R2337 T8565 T8564 amod related,pseudogenes
R2338 T8566 T8549 punct ", ",used
R2339 T8567 T8549 nsubj we,used
R2340 T8568 T8569 det a,kit
R2341 T8569 T8549 dobj kit,used
R2342 T8570 T8569 compound TOPO,kit
R2343 T8571 T8569 compound walker,kit
R2344 T8572 T8573 punct (,Invitrogen
R2345 T8573 T8569 parataxis Invitrogen,kit
R2346 T8574 T8573 punct ),Invitrogen
R2347 T8575 T8549 prep with,used
R2348 T8576 T8577 det the,primers
R2349 T8577 T8575 pobj primers,with
R2350 T8578 T8579 nmod pH34,T2L
R2351 T8579 T8577 nmod T2L,primers
R2352 T8580 T8579 punct -,T2L
R2353 T8581 T8582 punct (,3
R2354 T8582 T8579 parataxis 3,T2L
R2355 T8583 T8582 dep 5,3
R2356 T8584 T8583 punct ',5
R2357 T8585 T8582 punct -,3
R2358 T8586 T8582 dep ACTAGTCGCAGCAGGGATCCAGGAATATCT,3
R2359 T8587 T8582 punct -,3
R2360 T8588 T8582 punct ',3
R2361 T8589 T8582 punct ),3
R2362 T8590 T8579 cc and,T2L
R2363 T8591 T8592 compound pH34,L394
R2364 T8592 T8579 conj L394,T2L
R2365 T8593 T8592 punct -,L394
R2366 T8594 T8549 punct .,used
R2367 T8596 T8597 det The,sequence
R2368 T8597 T8599 nsubjpass sequence,cloned
R2369 T8598 T8597 amod resulting,sequence
R2370 T8600 T8599 auxpass was,cloned
R2371 T8601 T8599 prep into,cloned
R2372 T8602 T8601 pobj pCR2.1,into
R2373 T8603 T8604 punct (,Invitrogen
R2374 T8604 T8602 parataxis Invitrogen,pCR2.1
R2375 T8605 T8604 punct ),Invitrogen
R2376 T8606 T8599 punct .,cloned
R2377 T8608 T8609 nsubj We,obtained
R2378 T8609 T8610 ccomp obtained,cloned
R2379 T8611 T8612 det a,band
R2380 T8612 T8609 dobj band,obtained
R2381 T8613 T8614 punct ~,6
R2382 T8614 T8615 nummod 6,kbp
R2383 T8615 T8612 compound kbp,band
R2384 T8616 T8609 prep from,obtained
R2385 T8617 T8618 det the,library
R2386 T8618 T8616 pobj library,from
R2387 T8619 T8620 npadvmod NsiI,digensted
R2388 T8620 T8618 amod digensted,library
R2389 T8621 T8620 punct -,digensted
R2390 T8622 T8610 punct ;,cloned
R2391 T8623 T8624 npadvmod XbaI,digested
R2392 T8624 T8636 amod digested,fragments
R2393 T8625 T8623 punct -,XbaI
R2394 T8626 T8623 punct ", ",XbaI
R2395 T8627 T8623 conj SpeI,XbaI
R2396 T8628 T8627 punct -,SpeI
R2397 T8629 T8627 punct ", ",SpeI
R2398 T8630 T8627 conj EcoRI,SpeI
R2399 T8631 T8630 punct -,EcoRI
R2400 T8632 T8630 punct ", ",EcoRI
R2401 T8633 T8630 cc and,EcoRI
R2402 T8634 T8630 conj PstI,EcoRI
R2403 T8635 T8624 punct -,digested
R2404 T8636 T8610 nsubjpass fragments,cloned
R2405 T8637 T8636 prep of,fragments
R2406 T8638 T8639 det this,band
R2407 T8639 T8637 pobj band,of
R2408 T8640 T8610 auxpass were,cloned
R2409 T8641 T8610 prep into,cloned
R2410 T8642 T8643 compound pBluescript,KS
R2411 T8643 T8641 pobj KS,into
R2412 T8644 T8643 punct (,KS
R2413 T8645 T8643 punct -,KS
R2414 T8646 T8643 punct ),KS
R2415 T8647 T8610 prep for,cloned
R2416 T8648 T8647 pobj sequencing,for
R2417 T8649 T8610 punct .,cloned
R2418 T8651 T8652 det This,fragment
R2419 T8652 T8653 nsubj fragment,contained
R2420 T8654 T8655 det the,region
R2421 T8655 T8653 dobj region,contained
R2422 T8656 T8655 nummod 5,region
R2423 T8657 T8656 punct ',5
R2424 T8658 T8655 compound flanking,region
R2425 T8659 T8655 prep of,region
R2426 T8660 T8661 det the,gene
R2427 T8661 T8659 pobj gene,of
R2428 T8662 T8661 compound ESG1,gene
R2429 T8663 T8653 punct .,contained
R2430 T8665 T8666 det A,fragment
R2431 T8666 T8670 nsubjpass fragment,cloned
R2432 T8667 T8668 punct ~,3
R2433 T8668 T8669 nummod 3,kbp
R2434 T8669 T8666 compound kbp,fragment
R2435 T8671 T8666 punct ", ",fragment
R2436 T8672 T8666 acl obtained,fragment
R2437 T8673 T8672 prep from,obtained
R2438 T8674 T8675 det the,library
R2439 T8675 T8673 pobj library,from
R2440 T8676 T8677 npadvmod SacI,digested
R2441 T8677 T8675 amod digested,library
R2442 T8678 T8677 punct -,digested
R2443 T8679 T8670 punct ", ",cloned
R2444 T8680 T8670 auxpass was,cloned
R2445 T8681 T8670 prep into,cloned
R2446 T8682 T8681 pobj pCR2.1,into
R2447 T8683 T8670 prep for,cloned
R2448 T8684 T8683 pobj sequencing,for
R2449 T8685 T8670 punct .,cloned
R2450 T8687 T8688 det This,fragment
R2451 T8688 T8689 nsubjpass fragment,contained
R2452 T8690 T8689 auxpass was,contained
R2453 T8691 T8692 det the,region
R2454 T8692 T8689 dobj region,contained
R2455 T8693 T8692 nummod 5,region
R2456 T8694 T8693 punct ',5
R2457 T8695 T8692 acl flanking,region
R2458 T8696 T8697 det the,pseudogene
R2459 T8697 T8695 dobj pseudogene,flanking
R2460 T8698 T8689 punct .,contained