> top > docs > PMC:1420271 > annotations

PMC:1420271 JSONTXT

Annnotations TAB JSON ListView MergeView

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T621 46-51 NCBITaxon:10088 denotes mouse
T623 66-70 PR:000006847 denotes ESG1
T624 72-76 PR:000006847 denotes PH34
T625 77-82 PR:000006847 denotes ECAT2
T626 83-88 PR:000006847 denotes DPPA5
T627 111-120 UBERON:0000922 denotes Embryonic
T628 111-130 CL:0002322 denotes Embryonic stem cell
T629 111-144 _FRAGMENT denotes Embryonic stem cell-specific gene
T630 151-152 PR:000006847 denotes 1
T631 140-144 SO:0000704 denotes gene
T632 146-149 _FRAGMENT denotes ESG
T633 173-179 SO:0000417 denotes domain
T634 216-225 GO:0010467 denotes expressed
T635 229-242 UBERON:0019248 denotes early embryos
T636 244-254 CL:0000586 denotes germ cells
T637 260-269 UBERON:0000922 denotes embryonic
T638 260-274 _FRAGMENT denotes embryonic stem
T639 280-285 CL:0002322 denotes cells
T640 276-278 _FRAGMENT denotes ES
T641 315-329 SO:0000040 denotes genomic clones
T642 345-350 NCBITaxon:10088 denotes mouse
T643 351-355 PR:000006847 denotes ESG1
T644 356-360 SO:0000704 denotes gene
T645 370-381 SO:0000336 denotes pseudogenes
T646 504-509 NCBITaxon:10088 denotes mouse
T647 510-517 SO:0001026 denotes genomic
T648 549-553 PR:000006847 denotes ESG1
T649 554-558 SO:0000704 denotes gene
T650 582-591 NCBITaxon:2 denotes bacterial
T651 625-630 NCBITaxon:10088 denotes mouse
T652 631-635 PR:000006847 denotes ESG1
T653 636-640 SO:0000704 denotes gene
T654 659-663 PR:000006847 denotes ESG1
T655 693-697 SO:0000704 denotes gene
T656 731-735 PR:000006847 denotes ESG1
T657 827-837 SO:0000336 denotes pseudogene
T658 843-861 SO:0001416 denotes 5' flanking region
T659 869-873 PR:000006847 denotes ESG1
T660 874-878 SO:0000704 denotes gene
T661 900-910 SO:0000336 denotes pseudogene
T662 929-937 SO:0000165 denotes enhancer
T663 942-950 SO:0000167 denotes promoter
T664 980-988 CL:0002322 denotes ES cells
T665 1063-1067 PR:000006847 denotes ESG1
T666 1068-1072 SO:0000704 denotes gene
T667 1103-1111 CL:0002322 denotes ES cells
T668 1125-1133 SO:0005853 denotes cassette
T669 1180-1190 GO:0010467 denotes expression
T670 1194-1207 UBERON:0019248 denotes early embryos
T671 1212-1222 CL:0000586 denotes germ cells
T672 1224-1228 PR:000006847 denotes ESG1
T673 1232-1236 NCBITaxon:10088 denotes mice
T674 1292-1296 PR:000006847 denotes ESG1
T675 1300-1308 CL:0002322 denotes ES cells
T676 1381-1392 UBERON:0000358 denotes blastocysts
T677 1498-1502 PR:000006847 denotes ESG1
T678 1525-1533 CL:0002322 denotes ES cells
T679 1566-1579 GO:0008283 denotes proliferation
T680 1618-1623 NCBITaxon:10088 denotes mouse
T681 1624-1628 PR:000006847 denotes ESG1
T682 1629-1633 SO:0000704 denotes gene
T683 1662-1672 SO:0000336 denotes pseudogene
T684 1723-1733 GO:0010467 denotes expression
T685 1759-1769 CL:0000586 denotes germ cells
T686 1771-1775 PR:000006847 denotes ESG1
T687 1795-1810 _FRAGMENT denotes self-renewal of
T688 1814-1819 GO:0017145 denotes cells
T689 1811-1819 CL:0002322 denotes ES cells
T690 1824-1850 GO:0007281 denotes establishment of germcells
T691 1841-1850 CL:0000586 denotes germcells
T2001 1864-1873 UBERON:0000922 denotes Embryonic
T2002 1864-1878 _FRAGMENT denotes Embryonic stem
T2003 1884-1889 CL:0002322 denotes cells
T2004 1880-1882 _FRAGMENT denotes ES
T2005 1918-1929 UBERON:0000358 denotes blastocysts
T2006 1933-1937 NCBITaxon:10088 denotes mice
T2007 1956-1962 NCBITaxon:9606 denotes humans
T2008 1976-1984 CL:0002322 denotes ES cells
T2009 2131-2144 GO:0008283 denotes proliferation
T2010 2173-2178 NCBITaxon:10088 denotes mouse
T2011 2179-2187 CL:0002322 denotes ES cells
T2012 2214-2218 SO:0000704 denotes gene
T2013 2266-2271 NCBITaxon:9606 denotes human
T2014 2272-2280 CL:0002322 denotes ES cells
T2015 2370-2381 UBERON:0002240 denotes spinal cord
T2016 2477-2493 _FRAGMENT denotes proliferation of
T2017 2497-2502 GO:0008283 denotes cells
T2018 2494-2502 CL:0002322 denotes ES cells
T2019 2547-2556 CL:0000034 denotes stem cell
T2020 2584-2593 CHEBI:36357 denotes molecules
T2021 2607-2615 CL:0002322 denotes ES cells
T2022 2702-2707 SO:0000704 denotes genes
T2023 2721-2730 GO:0010467 denotes expressed
T2024 2734-2742 CL:0002322 denotes ES cells
T2025 2747-2760 UBERON:0019248 denotes early embryos
T2026 2820-2829 GO:0010467 denotes expressed
T2027 2820-2842 SO:0000345 denotes expressed sequence tag
T2028 2886-2897 SO:0000673 denotes transcripts
T2029 2909-2913 PR:000006847 denotes ESG1
T2030 2939-2944 PR:000006847 denotes dppa5
T2031 2948-2953 PR:000006847 denotes ECAT2
T2032 2955-2959 PR:000006847 denotes ESG1
T2033 2991-3001 SO:0000673 denotes transcript
T2034 3002-3006 PR:000006847 denotes Ph34
T2035 3034-3047 CHEBI:26536 denotes retinoic acid
T2036 3051-3060 UBERON:0000922 denotes embryonic
T2037 3051-3060 _FRAGMENT denotes embryonic
T2038 3071-3076 CL:0002321 denotes cells
T2039 3087-3097 GO:0010467 denotes expression
T2040 3106-3110 SO:0000704 denotes gene
T2041 3127-3131 NCBITaxon:10088 denotes mice
T2042 3135-3148 UBERON:0019248 denotes early embryos
T2043 3153-3163 CL:0000586 denotes germ cells
T2050 3259-3270 _FRAGMENT denotes multipotent
T2051 3280-3290 CL:0000048 denotes stem cells
T2052 3271-3290 CL:0000014 denotes germline stem cells
T2053 3297-3301 PR:000006847 denotes ESG1
T2054 3369-3375 SO:0000417 denotes domain
T2055 3401-3407 SO:0000417 denotes domain
T2056 3446-3450 PR:000006847 denotes ESG1
T2057 3512-3520 CL:0002322 denotes ES cells
T2058 3525-3529 NCBITaxon:10088 denotes mice
T2059 3540-3547 SO:0001026 denotes genomic
T2060 3580-3594 SO:0000040 denotes genomic clones
T2061 3610-3615 NCBITaxon:10088 denotes mouse
T2062 3616-3620 PR:000006847 denotes ESG1
T2063 3621-3625 SO:0000704 denotes gene
T2064 3636-3647 SO:0000336 denotes pseudogenes
T2065 3667-3678 SO:0000336 denotes pseudogenes
T2066 3697-3701 SO:0000147 denotes exon
T2067 3702-3708 SO:0000188 denotes intron
T2068 3726-3730 PR:000006847 denotes ESG1
T2069 3731-3735 SO:0000704 denotes gene
T2070 3768-3772 SO:0000704 denotes gene
T2071 3805-3816 SO:0000336 denotes pseudogenes
T2072 3837-3844 SO:0000188 denotes introns
T2073 3886-3904 GO:0032197 denotes retrotransposition
T2074 3908-3912 PR:000006847 denotes ESG1
T2075 3913-3924 SO:0000673 denotes transcripts
T2076 3963-3968 NCBITaxon:10088 denotes mouse
T2077 3969-3973 PR:000006847 denotes ESG1
T2078 3974-3978 SO:0000704 denotes gene
T2079 3983-3994 SO:0000336 denotes pseudogenes
T2080 4079-4084 NCBITaxon:10088 denotes mouse
T2081 4085-4089 SO:0000704 denotes gene
T2082 4124-4135 SO:0000336 denotes pseudogenes
T2083 4155-4159 SO:0000704 denotes gene
T2084 4213-4217 PR:000006847 denotes ESG1
T3792 4287-4292 NCBITaxon:10088 denotes mouse
T3793 4293-4297 PR:000006847 denotes ESG1
T3794 4298-4302 SO:0000704 denotes gene
T3795 4307-4317 SO:0000336 denotes psedogenes
T3796 4368-4373 NCBITaxon:10088 denotes mouse
T3797 4374-4378 PR:000006847 denotes ESG1
T3798 4379-4383 SO:0000704 denotes gene
T3799 4388-4399 SO:0000336 denotes pseudogenes
T3800 4438-4443 NCBITaxon:10088 denotes mouse
T3801 4444-4451 SO:0001026 denotes genomic
T3802 4470-4474 PR:000006847 denotes ESG1
T3803 4532-4543 SO:0000336 denotes pseudogenes
T3804 4552-4559 SO:0000188 denotes introns
T3805 4636-4642 SO:0000188 denotes intron
T3806 4648-4659 SO:0000336 denotes pseudogenes
T3807 4763-4775 SO:0000336 denotes pseudodgenes
T3808 4793-4801 SO:0000857 denotes homology
T3809 4805-4809 PR:000006847 denotes ESG1
T3810 4946-4952 SO:0000188 denotes intron
T3811 4958-4969 SO:0000336 denotes pseudogenes
T3812 5021-5037 SO:0000043 denotes retropseudogenes
T3813 5081-5086 SO:0000704 denotes genes
T3814 5396-5400 PR:000006847 denotes ESG1
T3815 5401-5405 SO:0000704 denotes gene
T3816 5433-5440 SO:0000188 denotes introns
T3817 5474-5479 SO:0000147 denotes exons
T3818 5530-5534 PR:000006847 denotes ESG1
T3819 5569-5573 SO:0000147 denotes exon
T3820 5623-5627 PR:000006847 denotes ESG1
T3821 5628-5632 SO:0000704 denotes gene
T3822 5741-5745 SO:0000147 denotes exon
T3823 5753-5757 PR:000006847 denotes ESG1
T3824 5758-5762 SO:0000704 denotes gene
T3825 5798-5802 PR:000006847 denotes ESG1
T3826 5908-5912 PR:000006847 denotes ESG1
T3827 5913-5917 SO:0000704 denotes gene
T3828 5935-5944 NCBITaxon:2 denotes bacterial
T3829 5935-5966 SO:0000153 denotes bacterial artificial chromosome
T3830 5968-5971 SO:0000153 denotes BAC
T3831 5995-6001 SO:0000112 denotes primer
T3832 6041-6045 SO:0000704 denotes gene
T3833 6055-6066 SO:0000336 denotes pseudogenes
T3834 6113-6116 SO:0000153 denotes BAC
T3835 6249-6253 PR:000006847 denotes ESG1
T3836 6296-6303 SO:0000188 denotes introns
T3837 6325-6331 SO:0000188 denotes intron
T3838 6408-6429 SO:0001421 denotes exon-intron junctions
T3876 8662-8673 SO:0000336 denotes pseudogenes
T3877 8744-8754 SO:0000336 denotes pseudogene
T6124 8784-8789 NCBITaxon:10088 denotes mouse
T6125 8790-8794 PR:000006847 denotes ESG1
T6126 8795-8799 SO:0000704 denotes gene
T6127 8825-8829 PR:000006847 denotes ESG1
T6128 8846-8850 SO:0000704 denotes gene
T6129 8882-8887 NCBITaxon:10088 denotes mouse
T6130 8888-8896 CL:0002322 denotes ES cells
T6131 8920-8925 SO:0000147 denotes exons
T6132 8954-8962 CHEBI:7507 denotes neomycin
T6133 8994-8999 SO:0000704 denotes genes
T6134 9015-9025 CHEBI:24753 denotes hygromycin
T6135 9036-9040 SO:0000704 denotes gene
T6136 9058-9075 SO:0001644 denotes targeting vectors
T6137 9108-9116 CL:0002322 denotes ES cells
T6138 9155-9162 CL:0002322 denotes ES cell
T6139 9221-9237 SO:0001644 denotes targeting vector
T6140 9375-9391 SO:0001644 denotes targeting vector
T6171 12032-12036 SO:0000704 denotes gene
T6172 12071-12075 PR:000006847 denotes ESG1
T6173 12079-12087 CL:0002322 denotes ES cells
T6174 12105-12115 GO:0010467 denotes expression
T6175 12119-12126 CL:0002322 denotes ES cell
T6176 12134-12139 SO:0000704 denotes genes
T6177 12149-12154 PR:000010968 denotes Nanog
T6178 12159-12163 PR:000013044 denotes Oct3
T6179 12181-12185 PR:000006847 denotes ESG1
T6180 12189-12197 CL:0002322 denotes ES cells
T6181 12219-12223 PR:000013044 denotes Oct3
T6182 12226-12236 GO:0010467 denotes expression
T6183 12296-12300 SO:0000704 denotes gene
T6184 12296-12311 GO:0010467 denotes gene expression
T6185 12350-12358 CL:0002322 denotes ES cells
T6186 12363-12367 PR:000006847 denotes ESG1
T6187 12371-12379 CL:0002322 denotes ES cells
T6188 12389-12394 SO:0000704 denotes genes
T6189 12442-12446 PR:000006847 denotes ESG1
T6190 12467-12471 PR:000009450 denotes Krt1
T6191 12475-12478 PR:000014397 denotes Pem
T6192 12480-12484 PR:000003172 denotes Ctgf
T6193 12486-12491 PR:000013428 denotes Ptgs2
T6194 12502-12507 PR:000000216 denotes Inhba
T6195 12515-12520 SO:0000704 denotes genes
T6196 12530-12539 GO:0065007 denotes regulated
T6197 12566-12570 PR:000006847 denotes ESG1
T6198 12578-12582 PR:000006847 denotes ESG1
T6199 12614-12620 SO:0000417 denotes domain
T6200 12653-12658 SO:0000704 denotes genes
T6201 13175-13176 NCBITaxon:10088 denotes e
T6202 13181-13185 PR:000006847 denotes ESG1
T6203 13195-13199 NCBITaxon:10088 denotes mice
T6204 13219-13223 PR:000006847 denotes ESG1
T6205 13227-13234 CL:0002322 denotes ES cell
T6206 13251-13262 UBERON:0000358 denotes blastocysts
T6207 13273-13277 NCBITaxon:10088 denotes mice
T6208 13344-13348 PR:000006847 denotes ESG1
T6209 13352-13356 NCBITaxon:10088 denotes mice
T6210 13399-13403 PR:000006847 denotes ESG1
T6211 13415-13419 PR:000006847 denotes ESG1
T6212 13445-13449 PR:000006847 denotes ESG1
T6213 13453-13457 NCBITaxon:10088 denotes mice
T6214 13465-13472 NCBITaxon:33208 denotes animals
T6215 13576-13582 UBERON:0000473 denotes testis
T6216 13587-13592 UBERON:0000992 denotes ovary
T6217 13672-13676 PR:000006847 denotes ESG1
T6218 13701-13706 NCBITaxon:10088 denotes mouse
T6219 13723-13732 CL:0000586 denotes germ cell
T6220 13723-13742 GO:0007281 denotes germ cell formation
T6221 13762-13770 CL:0002322 denotes ES cells
T6222 13776-13787 UBERON:0000358 denotes blastocysts
T6223 13816-13820 PR:000006847 denotes ESG1
T6224 13834-13838 PR:000006847 denotes ESG1
T6225 13864-13871 CL:0002322 denotes ES cell
T6226 13907-13911 PR:000006847 denotes ESG1
T6227 13922-13926 PR:000006847 denotes ESG1
T6228 13932-13940 CL:0002322 denotes ES cells
T6229 13973-13986 GO:0008283 denotes proliferation
T6230 14037-14041 PR:000006847 denotes ESG1
T6231 14060-14068 CL:0002322 denotes ES cells
T7334 14120-14124 PR:000006847 denotes ESG1
T7335 14144-14149 NCBITaxon:10088 denotes mouse
T7336 14150-14154 SO:0000704 denotes gene
T7337 14217-14225 CL:0002322 denotes ES cells
T7338 14244-14254 GO:0010467 denotes expression
T7339 14258-14271 UBERON:0019248 denotes early embryos
T7340 14273-14283 CL:0000586 denotes germ cells
T7341 14335-14339 PR:000006847 denotes ESG1
T7342 14359-14364 NCBITaxon:10088 denotes mouse
T7343 14378-14387 CL:0000586 denotes germ cell
T7344 14378-14397 GO:0007281 denotes germ cell formation
T7345 14403-14410 CL:0002322 denotes ES cell
T7346 14406-14423 GO:0017145 denotes cell self-renewal
T8140 14466-14469 SO:0000153 denotes BAC
T8141 14492-14497 NCBITaxon:10088 denotes mouse
T8142 14498-14502 PR:000006847 denotes ESG1
T8143 14503-14507 SO:0000704 denotes gene
T8144 14520-14529 NCBITaxon:2 denotes bacterial
T8145 14520-14551 SO:0000153 denotes bacterial artificial chromosome
T8146 14553-14556 SO:0000153 denotes BAC
T8147 14576-14581 NCBITaxon:10088 denotes mouse
T8148 14582-14586 PR:000006847 denotes ESG1
T8149 14587-14591 SO:0000704 denotes gene
T8150 14629-14634 NCBITaxon:10088 denotes mouse
T8151 14635-14638 SO:0000153 denotes BAC
T8152 14687-14691 PR:000006847 denotes pH34
T8153 14730-14734 PR:000006847 denotes pH34
T8154 14769-14776 SO:0000112 denotes primers
T8155 14857-14860 SO:0000153 denotes BAC
T8156 14896-14900 PR:000006847 denotes pH34
T8157 14946-14950 PR:000006847 denotes pH34
T8158 14985-14989 PR:000006847 denotes pH34
T8159 15035-15039 PR:000006847 denotes pH34
T8160 15082-15086 PR:000006847 denotes pH34
T8161 15134-15147 GO:0097617 denotes Hybridization
T8162 15203-15207 PR:000006847 denotes ESG1
T8163 15208-15212 SO:0000704 denotes gene
T8164 15216-15227 SO:0000336 denotes pseudogenes
T8165 15267-15272 NCBITaxon:10088 denotes mouse
T8166 15273-15277 PR:000006847 denotes ESG1
T8167 15278-15282 SO:0000704 denotes gene
T8168 15291-15309 SO:0001417 denotes 3' flanking region
T8169 15331-15333 SO:0000028 denotes bp
T8170 15370-15376 SO:0000440 denotes vector
T8171 15391-15398 PR:P43870 denotes HindIII
T8172 15436-15442 SO:0000440 denotes vector
T8173 15515-15519 PR:000006847 denotes ESG1
T8174 15520-15530 SO:0000336 denotes pseudogene
T8175 15539-15557 SO:0001417 denotes 3' flanking region
T8176 15565-15567 SO:0000028 denotes bp
T8177 15622-15627 PR:P23940 denotes BamHI
T8178 15656-15662 SO:0000440 denotes vector
T8179 15744-15763 SO:0001416 denotes 5' flanking regions
T8180 15771-15775 PR:000006847 denotes ESG1
T8181 15776-15780 SO:0000704 denotes gene
T8182 15797-15808 SO:0000336 denotes pseudogenes
T8183 15858-15862 PR:000006847 denotes pH34
T8184 15910-15914 PR:000006847 denotes pH34
T8185 15920-15927 SO:0000112 denotes primers
T8186 16007-16009 SO:0000028 denotes bp
T8187 16190-16208 SO:0001416 denotes 5' flanking region
T8188 16216-16220 PR:000006847 denotes ESG1
T8189 16221-16225 SO:0000704 denotes gene
T8190 16233-16235 SO:0000028 denotes bp
T8191 16368-16376 SO:0000357 denotes flanking
T8192 16381-16391 SO:0000336 denotes pseudogene
T9096 16410-16414 PR:000006847 denotes ESG1
T9097 16415-16432 SO:0001644 denotes targeting vectors
T9098 16456-16460 PR:000006847 denotes ESG1
T9099 16461-16466 SO:0000147 denotes exons
T9100 16476-16493 SO:0001644 denotes targeting vectors
T9101 16515-16519 SO:0000243 denotes IRES
T9102 16526-16534 SO:0005853 denotes cassette
T9103 16546-16550 SO:0000243 denotes IRES
T9104 16556-16564 SO:0005853 denotes cassette
T9105 16568-16576 SO:0000167 denotes promoter
T9106 16623-16625 SO:0000028 denotes bp
T9107 16660-16664 PR:000006847 denotes pH34
T9108 16718-16722 PR:000006847 denotes pH34
T9109 16776-16783 SO:0000112 denotes primers
T9110 16802-16804 SO:0000028 denotes bp
T9111 16830-16834 PR:000006847 denotes pH34
T9112 16888-16892 PR:000006847 denotes pH34
T9113 16942-16949 SO:0000112 denotes primers
T9114 16955-16959 SO:0000243 denotes IRES
T9115 16969-16973 SO:0000243 denotes IRES
T9116 16979-16988 SO:0005853 denotes cassettes
T9117 16994-17001 GO:0006266 denotes ligated
T9118 17051-17056 CHEBI:27026 denotes toxin
T9119 17059-17067 SO:0005853 denotes cassette
T9120 17143-17160 SO:0001644 denotes targeting vectors
T9121 17200-17208 CL:0002322 denotes ES cells
T9122 17222-17226 SO:0000704 denotes Gene
T9123 17244-17255 GO:0009294 denotes Transfected
T9124 17291-17295 CHEBI:42768 denotes G418
T9125 17309-17321 CHEBI:16976 denotes hygromycin B
T9126 17337-17344 SO:0001026 denotes Genomic
T9127 17354-17358 CHEBI:42768 denotes G418
T9128 17363-17375 CHEBI:16976 denotes hygromycin B
T9778 17513-17521 CL:0002322 denotes ES cells
T9779 17522-17529 SO:0001026 denotes genomic
T9780 17563-17573 GO:0019835 denotes Cell Lysis
T9781 17574-17582 CHEBI:75958 denotes Solution
T9782 17632-17639 SO:0001026 denotes genomic
T9783 17700-17707 CHEBI:2511 denotes agarose
T9784 17733-17738 CHEBI:25614 denotes nylon
T9785 17773-17775 SO:0000028 denotes bp
T9786 17895-17902 SO:0000112 denotes primers
T9787 17942-17944 SO:0000028 denotes bp
T9788 17982-17984 SO:0000028 denotes bp
T9789 18022-18024 SO:0000028 denotes bp
T9790 18051-18058 SO:0001026 denotes Genomic
T9791 18130-18132 SO:0000028 denotes bp
T9792 18260-18267 SO:0000112 denotes primers
T9793 18279-18289 GO:0097617 denotes hybridized
T9794 18302-18304 SO:0000028 denotes bp
T9795 18344-18346 SO:0000028 denotes bp
T9796 18385-18387 SO:0000028 denotes bp
T10358 18434-18438 PR:000006847 denotes ESG1
T10359 18450-18460 GO:0042571 denotes antibodies
T10360 18484-18488 PR:000006847 denotes Esg1
T10361 18519-18523 PR:000006847 denotes pH34
T10362 18576-18580 PR:000006847 denotes pH34
T10363 18629-18636 SO:0000112 denotes primers
T10364 18657-18661 PR:000006847 denotes pH34
T10365 18677-18688 SO:0000006 denotes PCR product
T10366 18737-18741 PR:000006847 denotes pH34
T10367 18840-18850 GO:0010467 denotes expression
T10368 18851-18857 SO:0000440 denotes vector
T10369 18866-18870 PR:000006847 denotes pH34
T10370 18884-18891 NCBITaxon:562 denotes E. coli
T10371 18918-18936 GO:0006412 denotes protein production
T10372 19007-19011 PR:000006847 denotes ESG1
T10373 19034-19055 CHEBI:44557 denotes nitrilotriacetic acid
T10374 19056-19063 CHEBI:2511 denotes agarose
T10375 19124-19128 CHEBI:16199 denotes urea
T10376 19157-19161 CHEBI:16199 denotes urea
T10377 19225-19232 NCBITaxon:9986 denotes rabbits
T10378 19250-19254 PR:000006847 denotes ESG1
T10379 19266-19276 GO:0042571 denotes antibodies
T10816 19313-19320 CL:0002322 denotes ES cell
T10817 19387-19409 CHEBI:8984 denotes sodium dodecyl sulfate
T10818 19411-19414 CHEBI:8984 denotes SDS
T10819 19459-19473 CHEBI:53325 denotes nitrocellulose
T10820 19532-19536 PR:000006847 denotes ESG1
T10821 19560-19564 PR:000013044 denotes Oct3
T10822 19607-19611 PR:000005257 denotes CDK4
T10823 19682-19692 GO:0042571 denotes antibodies
T10824 19694-19705 NCBITaxon:3704 denotes Horseradish
T10825 19717-19727 MOP:0000779 denotes conjugated
T10826 19733-19739 NCBITaxon:9986 denotes rabbit
T10827 19749-19754 NCBITaxon:10088 denotes mouse
T10828 19755-19770 GO:0019814 denotes immunoglobulins
T10829 19816-19824 GO:0042571 denotes antibody
T10830 19854-19862 GO:0042571 denotes antibody
T11412 19936-19940 PR:000006847 denotes ESG1
T11413 19951-19959 CL:0002322 denotes ES cells
T11414 19965-19976 UBERON:0000358 denotes blastocysts
T11415 19977-19981 PR:000006847 denotes Esg1
T11416 19987-19991 PR:000006847 denotes ESG1
T11417 20009-20013 NCBITaxon:10088 denotes mice
T11418 20033-20042 CHEBI:41774 denotes Tamoxifen
T11419 20055-20067 CHEBI:6716 denotes Depo-provera
T11420 20110-20119 GO:0007565 denotes pregnancy
T11421 20138-20145 UBERON:0000922 denotes embryos
T11422 20182-20188 UBERON:0000995 denotes uterus
T11423 20313-20319 NCBITaxon:27592 denotes Bovine
T11424 20320-20325 UBERON:0001977 denotes Serum
T11425 20423-20433 CHEBI:17334 denotes Penicillin
T11426 20434-20446 CHEBI:17076 denotes Streptomysin
T11427 20473-20490 CHEBI:41218 denotes 2-mercaptoethanol
T11428 20608-20615 PR:000027795 denotes trypsin
T11429 20760-20767 PR:000027795 denotes trypsin
T11430 20866-20873 _FRAGMENT denotes primary
T11431 20877-20881 CL:0000001 denotes cell
T11432 20874-20881 CL:0002322 denotes ES cell
T11433 20971-20982 UBERON:0000119 denotes cell layers
T11858 21095-21103 CL:0002322 denotes ES cells
T11859 21108-21112 PR:000006847 denotes ESG1
T11860 21116-21124 CL:0002322 denotes ES cells
T11861 21142-21145 CHEBI:37987 denotes Cy3
T11862 21150-21153 CHEBI:37989 denotes Cy5
T11863 21186-21196 GO:0097617 denotes hybridized
T11864 21202-21207 NCBITaxon:10088 denotes Mouse
T11865 21356-21369 GO:0097617 denotes Hybridization
T12038 5103-5107 PR:000006847 denotes ESG1
T12039 5107-5118 SO:0000336 denotes pseudogenes
T12040 5151-5156 NCBITaxon:10088 denotes mouse
T12041 5157-5164 SO:0001026 denotes genomic
T12042 5229-5235 SO:0000188 denotes Intron
T12043 5241-5244 SO:0000730 denotes gap
T12236 6780-6783 SO:0000153 denotes BAC
T12237 6806-6810 PR:000006847 denotes ESG1
T12238 6811-6815 SO:0000704 denotes gene
T12239 6834-6844 SO:0000336 denotes pseudogene
T12240 6846-6848 SO:0000336 denotes PS
T12241 6874-6878 SO:0000704 denotes gene
T12242 6883-6885 SO:0000336 denotes PS
T12243 6933-6937 SO:0000704 denotes gene
T12244 6942-6944 SO:0000336 denotes PS
T12245 6969-6979 SO:0000857 denotes homologous
T12246 6991-7001 SO:0000028 denotes base pairs
T12247 7024-7028 SO:0000147 denotes exon
T12248 7036-7038 SO:0000028 denotes bp
T12249 7063-7067 SO:0000147 denotes exon
T12508 7180-7188 SO:0000167 denotes Promoter
T12509 7189-7197 SO:0000165 denotes enhancer
T12510 7214-7218 PR:000006847 denotes ESG1
T12511 7219-7223 SO:0000704 denotes gene
T12512 7228-7238 SO:0000336 denotes pseudogene
T12513 7261-7263 SO:0000028 denotes bp
T12514 7282-7301 SO:0001416 denotes 5' flanking regions
T12515 7309-7313 SO:0000704 denotes gene
T12516 7318-7320 SO:0000336 denotes PS
T12517 7363-7371 SO:0000155 denotes plasmids
T12518 7400-7405 SO:0000704 denotes genes
T12519 7428-7436 CL:0002322 denotes ES cells
T12520 7453-7466 CHEBI:26536 denotes retinoic acid
T12521 7475-7483 CL:0002322 denotes ES cells
T13011 9430-9435 NCBITaxon:10088 denotes mouse
T13012 9436-9440 PR:000006847 denotes ESG1
T13013 9441-9445 SO:0000704 denotes gene
T13014 9519-9536 SO:0000409 denotes Recognition sites
T13015 9622-9626 SO:0000704 denotes gene
T13016 9647-9652 CHEBI:27026 denotes toxin
T13017 9695-9712 SO:0001644 denotes targeting vectors
T13018 9824-9832 CL:0002322 denotes ES cells
T13019 9847-9855 CL:0002322 denotes ES cells
T13020 9869-9877 CL:0002322 denotes ES cells
T13021 9884-9888 PR:000006847 denotes ESG1
T13022 9894-9902 CL:0002322 denotes ES cells
T13023 10008-10016 CL:0002322 denotes ES cells
T13024 10023-10027 PR:000006847 denotes ESG1
T13025 10033-10041 CL:0002322 denotes ES cells
T13026 10079-10087 CL:0002322 denotes ES cells
T13027 10201-10217 CHEBI:4883 denotes ethidium bromide
T13028 10230-10239 GO:0005840 denotes ribosomal
T13541 11333-11337 PR:000006847 denotes ESG1
T13542 11343-11351 CL:0002322 denotes ES cells
T13543 11374-11378 PR:000006847 denotes ESG1
T13544 11384-11391 CL:0002322 denotes ES cell
T13545 11401-11406 GO:0040007 denotes grown
T13546 11463-11467 PR:000006847 denotes ESG1
T13547 11502-11510 CL:0002322 denotes ES cells
T13548 11786-11790 PR:000006847 denotes ESG1
T13549 11796-11804 CL:0002322 denotes ES cells
T13550 11806-11817 CHEBI:51686 denotes hematoxylin
T13911 12728-12732 SO:0000704 denotes Gene
T13912 12728-12743 GO:0010467 denotes Gene expression
T13913 12756-12760 PR:000006847 denotes ESG1
T13914 12766-12774 CL:0002322 denotes ES cells
T13915 12829-12837 CL:0002322 denotes ES cells
T13916 12842-12846 PR:000006847 denotes ESG1
T13917 12852-12860 CL:0002322 denotes ES cells
T13918 12879-12882 CHEBI:37987 denotes Cy3
T13919 12887-12890 CHEBI:37989 denotes Cy5
T13920 12919-12929 GO:0097617 denotes hybridized
T13921 12941-12946 NCBITaxon:10088 denotes Mouse
T13922 13068-13072 PR:000006847 denotes ESG1
T13923 13080-13084 PR:000006847 denotes ESG1
T13924 13112-13122 GO:0010467 denotes expression
T13925 13126-13130 PR:000006847 denotes ESG1
T13926 13132-13136 PR:000013044 denotes Oct3
T13927 13143-13147 PR:000005257 denotes CDK4
T622 52-56 SO:0000704 denotes gene
T2044 3181-3190 GO:0010467 denotes expressed
T2045 3223-3231 CL:0002322 denotes ES cells
T2046 3233-3242 UBERON:0000922 denotes embryonic
T2047 3233-3242 _FRAGMENT denotes embryonic
T2048 3248-3253 CL:0002321 denotes cells
T2049 3243-3253 CL:0000586 denotes germ cells
T3839 6440-6458 SO:0001416 denotes 5' flanking region
T3840 6497-6505 SO:0000167 denotes promoter
T3841 6506-6514 SO:0000165 denotes enhancer
T3842 6574-6582 CL:0002322 denotes ES cells
T3843 6595-6608 CL:0002371 denotes somatic cells
T3844 6705-6718 CHEBI:26536 denotes retinoic acid
T3845 6759-6763 PR:000006847 denotes ESG1
T3846 6764-6768 SO:0000704 denotes gene
T3847 7648-7651 SO:0000153 denotes BAC
T3848 7677-7681 PR:000006847 denotes ESG1
T3849 7747-7749 SO:0000028 denotes bp
T3850 7750-7769 SO:0000605 denotes intergenic sequence
T3851 7813-7817 PR:000006847 denotes ESG1
T3852 7875-7880 SO:0000147 denotes exons
T3853 7885-7892 SO:0000188 denotes introns
T3854 7900-7904 PR:000006847 denotes ESG1
T3855 7905-7909 SO:0000704 denotes gene
T3856 7993-7998 SO:0000147 denotes exons
T3857 8031-8035 SO:0000147 denotes exon
T3858 8064-8074 SO:0000028 denotes base pairs
T3859 8082-8101 SO:0001417 denotes 3' flanking regions
T3860 8129-8133 PR:000006847 denotes ESG1
T3861 8134-8138 SO:0000704 denotes gene
T3862 8147-8157 SO:0000336 denotes pseudogene
T3863 8169-8179 SO:0000028 denotes base pairs
T3864 8187-8205 SO:0001416 denotes 5' flanking region
T3865 8227-8245 SO:0001416 denotes 5' flanking region
T3866 8251-8253 SO:0000028 denotes bp
T3867 8275-8283 SO:0000167 denotes promoter
T3868 8284-8292 SO:0000165 denotes enhancer
T3869 8401-8411 GO:0006412 denotes translated
T3870 8485-8495 SO:0000336 denotes pseudogene
T3871 8547-8558 SO:0000336 denotes pseudogenes
T3872 8572-8576 SO:0000147 denotes exon
T3873 8577-8583 SO:0000188 denotes intron
T3874 8604-8608 PR:000006847 denotes ESG1
T3875 8609-8613 SO:0000704 denotes gene
T6141 10296-10304 CL:0002322 denotes ES cells
T6142 10330-10336 SO:0000440 denotes vector
T6143 10360-10368 CL:0002322 denotes ES cells
T6144 10377-10381 CHEBI:42768 denotes G418
T6145 10432-10436 PR:000006847 denotes ESG1
T6146 10512-10516 PR:000006847 denotes ESG1
T6147 10569-10575 SO:0000440 denotes vector
T6148 10598-10604 SO:0000440 denotes vector
T6149 10687-10693 SO:0000440 denotes vector
T6150 10734-10738 PR:000006847 denotes ESG1
T6151 10742-10750 CL:0002322 denotes ES cells
T6152 10809-10821 GO:0008283 denotes proliferated
T6153 10915-10919 PR:000006847 denotes ESG1
T6154 10974-11000 PR:000009799 denotes leukemia inhibitory factor
T6155 11016-11029 CHEBI:26536 denotes retinoic acid
T6156 11081-11087 UBERON:0000180 denotes flanks
T6157 11096-11100 NCBITaxon:10088 denotes mice
T6158 11176-11187 UBERON:0000923 denotes germ layers
T6159 11229-11233 PR:000006847 denotes ESG1
T6160 11257-11269 GO:0017145 denotes self-renewal
T6161 11301-11309 CL:0002322 denotes ES cells
T6162 11840-11841 CL:0002322 denotes e
T6163 11841-11842 CHEBI:51686 denotes x
T6164 11853-11857 SO:0000704 denotes gene
T6165 11853-11868 GO:0010467 denotes gene expression
T6166 11881-11885 PR:000006847 denotes ESG1
T6167 11889-11897 CL:0002322 denotes ES cells
T6168 11963-11968 SO:0000704 denotes genes
T6169 11995-12003 CL:0002322 denotes ES cells
T6170 12005-12009 PR:000006847 denotes ESG1
R3166 T11431 T11430 _lexicallyChainedTo cell,primary
R1 T630 T629 _lexicallyChainedTo 1,Embryonic stem cell-specific gene
R2 T630 T632 _lexicallyChainedTo 1,ESG
R3 T639 T638 _lexicallyChainedTo cells,embryonic stem
R4 T639 T640 _lexicallyChainedTo cells,ES
R5 T688 T687 _lexicallyChainedTo cells,self-renewal of
R313 T2003 T2002 _lexicallyChainedTo cells,Embryonic stem
R314 T2003 T2004 _lexicallyChainedTo cells,ES
R315 T2017 T2016 _lexicallyChainedTo cells,proliferation of
R316 T2038 T2037 _lexicallyChainedTo cells,embryonic
R317 T2048 T2047 _lexicallyChainedTo cells,embryonic
R318 T2051 T2050 _lexicallyChainedTo stem cells,multipotent

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T692 46-51 NCBITaxon:10088 denotes mouse
T693 52-56 SO_EXT:0000704 denotes gene
T694 57-65 SO_EXT:sequence_coding_function denotes encoding
T695 66-70 PR_EXT:000006847 denotes ESG1
T696 72-76 PR_EXT:000006847 denotes PH34
T697 77-82 PR_EXT:000006847 denotes ECAT2
T698 83-88 PR_EXT:000006847 denotes DPPA5
T699 111-120 UBERON:0000922 denotes Embryonic
T700 111-130 CL:0002322 denotes Embryonic stem cell
T701 111-144 _FRAGMENT denotes Embryonic stem cell-specific gene
T702 151-152 PR_EXT:000006847 denotes 1
T703 126-130 CL_GO_EXT:cell denotes cell
T704 140-144 SO_EXT:0000704 denotes gene
T705 146-149 _FRAGMENT denotes ESG
T706 160-167 SO_EXT:sequence_coding_function denotes encodes
T707 173-179 SO_EXT:0000417 denotes domain
T708 191-198 CHEBI_PR_EXT:protein denotes protein
T709 216-225 GO:0010467 denotes expressed
T710 229-242 UBERON:0019248 denotes early embryos
T711 244-254 CL:0000586 denotes germ cells
T712 249-254 CL_GO_EXT:cell denotes cells
T713 260-269 UBERON:0000922 denotes embryonic
T714 260-274 _FRAGMENT denotes embryonic stem
T715 280-285 CL:0002322 denotes cells
T716 276-278 _FRAGMENT denotes ES
T717 280-285 CL_GO_EXT:cell denotes cells
T718 315-329 SO_EXT:0000040 denotes genomic clones
T719 345-350 NCBITaxon:10088 denotes mouse
T720 351-355 PR_EXT:000006847 denotes ESG1
T721 356-360 SO_EXT:0000704 denotes gene
T722 370-381 SO_EXT:0000336 denotes pseudogenes
T723 398-409 GO_SO_EXT:chromosome denotes chromosomal
T724 410-423 GO_PATO_EXT:biological_localization_process_or_quality denotes localizations
T725 427-440 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T726 504-509 NCBITaxon:10088 denotes mouse
T727 510-517 SO_EXT:0001026 denotes genomic
T728 549-553 PR_EXT:000006847 denotes ESG1
T729 554-558 SO_EXT:0000704 denotes gene
T730 582-591 NCBITaxon:2 denotes bacterial
T731 603-609 SO_EXT:sequence_cloned_entity denotes clones
T732 625-630 NCBITaxon:10088 denotes mouse
T733 631-635 PR_EXT:000006847 denotes ESG1
T734 636-640 SO_EXT:0000704 denotes gene
T735 659-663 PR_EXT:000006847 denotes ESG1
T736 669-677 SO_EXT:biological_sequence denotes sequence
T737 693-697 SO_EXT:0000704 denotes gene
T738 713-723 GO_SO_EXT:chromosome denotes chromosome
T739 731-735 PR_EXT:000006847 denotes ESG1
T740 741-749 SO_EXT:biological_sequence denotes sequence
T741 780-789 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T742 816-826 SO_EXT:sequence_duplication_process denotes duplicated
T743 827-837 SO_EXT:0000336 denotes pseudogene
T744 843-861 SO_EXT:0001416 denotes 5' flanking region
T745 869-873 PR_EXT:000006847 denotes ESG1
T746 874-878 SO_EXT:0000704 denotes gene
T747 900-910 SO_EXT:0000336 denotes pseudogene
T748 929-937 SO_EXT:0000165 denotes enhancer
T749 942-950 SO_EXT:0000167 denotes promoter
T750 965-979 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T751 980-988 CL:0002322 denotes ES cells
T752 983-988 CL_GO_EXT:cell denotes cells
T753 992-1002 GO_EXT:0045289 denotes luciferase
T754 1032-1045 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T755 1063-1067 PR_EXT:000006847 denotes ESG1
T756 1068-1072 SO_EXT:0000704 denotes gene
T757 1077-1085 SO_EXT:sequence_substitution_process denotes replaced
T758 1091-1099 SO_EXT:biological_sequence denotes sequence
T759 1103-1111 CL:0002322 denotes ES cells
T760 1106-1111 CL_GO_EXT:cell denotes cells
T761 1119-1124 GO_EXT:beta_geo denotes β-geo
T762 1125-1133 SO_EXT:0005853 denotes cassette
T763 1148-1161 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T764 1180-1190 GO:0010467 denotes expression
T765 1194-1207 UBERON:0019248 denotes early embryos
T766 1212-1222 CL:0000586 denotes germ cells
T767 1217-1222 CL_GO_EXT:cell denotes cells
T768 1224-1228 PR_EXT:000006847 denotes ESG1
T769 1228-1229 SO_EXT:sequence_nullness_or_absence denotes -
T770 1230-1231 SO_EXT:sequence_nullness_or_absence denotes -
T771 1232-1236 NCBITaxon:10088 denotes mice
T772 1292-1296 PR_EXT:000006847 denotes ESG1
T773 1296-1297 SO_EXT:sequence_nullness_or_absence denotes -
T774 1298-1299 SO_EXT:sequence_nullness_or_absence denotes -
T775 1300-1308 CL:0002322 denotes ES cells
T776 1303-1308 CL_GO_EXT:cell denotes cells
T777 1349-1362 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T778 1381-1392 UBERON:0000358 denotes blastocysts
T779 1498-1502 PR_EXT:000006847 denotes ESG1
T780 1512-1517 CL_GO_EXT:cell denotes cells
T781 1525-1533 CL:0002322 denotes ES cells
T782 1528-1533 CL_GO_EXT:cell denotes cells
T783 1566-1579 GO:0008283 denotes proliferation
T784 1585-1600 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T785 1618-1623 NCBITaxon:10088 denotes mouse
T786 1624-1628 PR_EXT:000006847 denotes ESG1
T787 1629-1633 SO_EXT:0000704 denotes gene
T788 1651-1661 SO_EXT:sequence_duplication_process denotes duplicated
T789 1662-1672 SO_EXT:0000336 denotes pseudogene
T790 1688-1698 GO_SO_EXT:chromosome denotes chromosome
T791 1723-1733 GO:0010467 denotes expression
T792 1749-1754 CL_GO_EXT:cell denotes cells
T793 1759-1769 CL:0000586 denotes germ cells
T794 1764-1769 CL_GO_EXT:cell denotes cells
T795 1771-1775 PR_EXT:000006847 denotes ESG1
T796 1795-1810 _FRAGMENT denotes self-renewal of
T797 1814-1819 GO:0017145 denotes cells
T798 1811-1819 CL:0002322 denotes ES cells
T799 1814-1819 CL_GO_EXT:cell denotes cells
T800 1824-1850 GO:0007281 denotes establishment of germcells
T801 1841-1850 CL:0000586 denotes germcells
T2085 1864-1873 UBERON:0000922 denotes Embryonic
T2086 1864-1878 _FRAGMENT denotes Embryonic stem
T2087 1884-1889 CL:0002322 denotes cells
T2088 1880-1882 _FRAGMENT denotes ES
T2089 1884-1889 CL_GO_EXT:cell denotes cells
T2090 1918-1929 UBERON:0000358 denotes blastocysts
T2091 1933-1937 NCBITaxon:10088 denotes mice
T2092 1956-1962 NCBITaxon:9606 denotes humans
T2093 1976-1984 CL:0002322 denotes ES cells
T2094 1979-1984 CL_GO_EXT:cell denotes cells
T2095 2077-2090 GO_RO_EXT:developmental_differentiation_process denotes differentiate
T2096 2114-2119 CL_GO_EXT:cell denotes cells
T2097 2131-2144 GO:0008283 denotes proliferation
T2098 2173-2178 NCBITaxon:10088 denotes mouse
T2099 2179-2187 CL:0002322 denotes ES cells
T2100 2182-2187 CL_GO_EXT:cell denotes cells
T2101 2214-2218 SO_EXT:0000704 denotes gene
T2102 2266-2271 NCBITaxon:9606 denotes human
T2103 2272-2280 CL:0002322 denotes ES cells
T2104 2275-2280 CL_GO_EXT:cell denotes cells
T2105 2304-2308 CL_GO_EXT:cell denotes cell
T2106 2370-2381 UBERON:0002240 denotes spinal cord
T2107 2395-2403 PATO_UBERON_EXT:juvenile_organism denotes juvenile
T2108 2418-2427 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T2109 2477-2493 _FRAGMENT denotes proliferation of
T2110 2497-2502 GO:0008283 denotes cells
T2111 2494-2502 CL:0002322 denotes ES cells
T2112 2497-2502 CL_GO_EXT:cell denotes cells
T2113 2547-2556 CL:0000034 denotes stem cell
T2114 2552-2556 CL_GO_EXT:cell denotes cell
T2115 2557-2564 CHEBI_GO_EXT:biological_process_or_quality_or_role denotes biology
T2116 2584-2593 CHEBI:36357 denotes molecules
T2117 2607-2615 CL:0002322 denotes ES cells
T2118 2610-2615 CL_GO_EXT:cell denotes cells
T2119 2702-2707 SO_EXT:0000704 denotes genes
T2120 2721-2730 GO:0010467 denotes expressed
T2121 2734-2742 CL:0002322 denotes ES cells
T2122 2737-2742 CL_GO_EXT:cell denotes cells
T2123 2747-2760 UBERON:0019248 denotes early embryos
T2124 2788-2791 CHEBI_SO_EXT:DNA denotes DNA
T2125 2820-2829 GO:0010467 denotes expressed
T2126 2820-2842 SO_EXT:0000345 denotes expressed sequence tag
T2127 2839-2842 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes tag
T2128 2886-2897 SO_EXT:0000673 denotes transcripts
T2129 2909-2913 PR_EXT:000006847 denotes ESG1
T2130 2939-2944 PR_EXT:000006847 denotes dppa5
T2131 2948-2953 PR_EXT:000006847 denotes ECAT2
T2132 2955-2959 PR_EXT:000006847 denotes ESG1
T2133 2991-3001 SO_EXT:0000673 denotes transcript
T2134 3002-3006 PR_EXT:000006847 denotes Ph34
T2135 3016-3030 GO_EXT:negative_regulation denotes down-regulated
T2136 3034-3047 CHEBI:26536 denotes retinoic acid
T2137 3051-3060 UBERON:0000922 denotes embryonic
T2138 3051-3060 _FRAGMENT denotes embryonic
T2139 3071-3076 CL:0002321 denotes cells
T2140 3071-3076 CL_GO_EXT:cell denotes cells
T2141 3087-3097 GO:0010467 denotes expression
T2142 3106-3110 SO_EXT:0000704 denotes gene
T2143 3127-3131 NCBITaxon:10088 denotes mice
T2144 3135-3148 UBERON:0019248 denotes early embryos
T2145 3153-3163 CL:0000586 denotes germ cells
T2146 3158-3163 CL_GO_EXT:cell denotes cells
T2147 3181-3190 GO:0010467 denotes expressed
T2148 3206-3211 CL_GO_EXT:cell denotes cells
T2149 3223-3231 CL:0002322 denotes ES cells
T2150 3226-3231 CL_GO_EXT:cell denotes cells
T2151 3233-3242 UBERON:0000922 denotes embryonic
T2152 3233-3242 _FRAGMENT denotes embryonic
T2153 3248-3253 CL:0002321 denotes cells
T2154 3243-3253 CL:0000586 denotes germ cells
T2155 3248-3253 CL_GO_EXT:cell denotes cells
T2156 3259-3270 _FRAGMENT denotes multipotent
T2157 3280-3290 CL:0000048 denotes stem cells
T2158 3271-3290 CL:0000014 denotes germline stem cells
T2159 3285-3290 CL_GO_EXT:cell denotes cells
T2160 3297-3301 PR_EXT:000006847 denotes ESG1
T2161 3302-3309 SO_EXT:sequence_coding_function denotes encodes
T2162 3312-3323 CHEBI_SO_EXT:polypeptide denotes polypeptide
T2163 3331-3342 CHEBI_SO_EXT:amino_acid denotes amino acids
T2164 3369-3375 SO_EXT:0000417 denotes domain
T2165 3389-3392 CHEBI_SO_EXT:RNA denotes RNA
T2166 3393-3400 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T2167 3401-3407 SO_EXT:0000417 denotes domain
T2168 3446-3450 PR_EXT:000006847 denotes ESG1
T2169 3467-3470 CHEBI_SO_EXT:RNA denotes RNA
T2170 3471-3478 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T2171 3479-3486 CHEBI_PR_EXT:protein denotes protein
T2172 3512-3520 CL:0002322 denotes ES cells
T2173 3515-3520 CL_GO_EXT:cell denotes cells
T2174 3525-3529 NCBITaxon:10088 denotes mice
T2175 3540-3547 SO_EXT:0001026 denotes genomic
T2176 3580-3594 SO_EXT:0000040 denotes genomic clones
T2177 3610-3615 NCBITaxon:10088 denotes mouse
T2178 3616-3620 PR_EXT:000006847 denotes ESG1
T2179 3621-3625 SO_EXT:0000704 denotes gene
T2180 3636-3647 SO_EXT:0000336 denotes pseudogenes
T2181 3667-3678 SO_EXT:0000336 denotes pseudogenes
T2182 3697-3701 SO_EXT:0000147 denotes exon
T2183 3702-3708 SO_EXT:0000188 denotes intron
T2184 3726-3730 PR_EXT:000006847 denotes ESG1
T2185 3731-3735 SO_EXT:0000704 denotes gene
T2186 3768-3772 SO_EXT:0000704 denotes gene
T2187 3773-3784 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T2188 3805-3816 SO_EXT:0000336 denotes pseudogenes
T2189 3837-3844 SO_EXT:0000188 denotes introns
T2190 3886-3904 SO_EXT:sequence_retrotransposition_entity_or_process denotes retrotransposition
T2191 3886-3904 GO:0032197 denotes retrotransposition
T2192 3908-3912 PR_EXT:000006847 denotes ESG1
T2193 3913-3924 SO_EXT:0000673 denotes transcripts
T2194 3930-3941 GO_SO_EXT:chromosome denotes chromosomal
T2195 3942-3955 GO_PATO_EXT:biological_localization_process_or_quality denotes localizations
T2196 3963-3968 NCBITaxon:10088 denotes mouse
T2197 3969-3973 PR_EXT:000006847 denotes ESG1
T2198 3974-3978 SO_EXT:0000704 denotes gene
T2199 3983-3994 SO_EXT:0000336 denotes pseudogenes
T2200 4079-4084 NCBITaxon:10088 denotes mouse
T2201 4085-4089 SO_EXT:0000704 denotes gene
T2202 4090-4098 SO_EXT:sequence_coding_function denotes encoding
T2203 4104-4111 CHEBI_PR_EXT:protein denotes protein
T2204 4124-4135 SO_EXT:0000336 denotes pseudogenes
T2205 4155-4159 SO_EXT:0000704 denotes gene
T2206 4187-4200 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T2207 4213-4217 PR_EXT:000006847 denotes ESG1
T3878 4244-4255 GO_SO_EXT:chromosome denotes Chromosomal
T3879 4256-4268 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3880 4287-4292 NCBITaxon:10088 denotes mouse
T3881 4293-4297 PR_EXT:000006847 denotes ESG1
T3894 4480-4488 SO_EXT:biological_sequence denotes sequence
T3895 4532-4543 SO_EXT:0000336 denotes pseudogenes
T3896 4552-4559 SO_EXT:0000188 denotes introns
T3897 4563-4574 GO_SO_EXT:chromosome denotes chromosomes
T3898 4636-4642 SO_EXT:0000188 denotes intron
T3899 4648-4659 SO_EXT:0000336 denotes pseudogenes
T3900 4679-4682 CHEBI_SO_EXT:DNA denotes DNA
T3901 4707-4718 GO_SO_EXT:chromosome denotes chromosomal
T3902 4719-4731 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3903 4763-4775 SO_EXT:0000336 denotes pseudodgenes
T3904 4793-4801 SO:0000857 denotes homology
T3905 4805-4809 PR_EXT:000006847 denotes ESG1
T3906 4810-4814 SO_EXT:cDNA denotes cDNA
T3907 4850-4858 CHEBI_PR_EXT:protein denotes proteins
T3908 4880-4889 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T3909 4946-4952 SO_EXT:0000188 denotes intron
T3910 4958-4969 SO_EXT:0000336 denotes pseudogenes
T3911 5021-5037 SO_EXT:0000043 denotes retropseudogenes
T3912 5067-5071 CL_GO_EXT:cell denotes cell
T3913 5081-5086 SO_EXT:0000704 denotes genes
T3914 5338-5348 GO_SO_EXT:chromosome denotes chromosome
T3915 5368-5371 CHEBI_SO_EXT:DNA denotes DNA
T3916 5396-5400 PR_EXT:000006847 denotes ESG1
T3917 5401-5405 SO_EXT:0000704 denotes gene
T3918 5433-5440 SO_EXT:0000188 denotes introns
T3919 5474-5479 SO_EXT:0000147 denotes exons
T3920 5513-5522 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T3921 5530-5534 PR_EXT:000006847 denotes ESG1
T3922 5535-5539 SO_EXT:cDNA denotes cDNA
T3923 5540-5548 SO_EXT:biological_sequence denotes sequence
T3924 5569-5573 SO_EXT:0000147 denotes exon
T3925 5623-5627 PR_EXT:000006847 denotes ESG1
T3926 5628-5632 SO_EXT:0000704 denotes gene
T3927 5642-5652 GO_SO_EXT:chromosome denotes chromosome
T3928 5678-5681 CHEBI_SO_EXT:DNA denotes DNA
T3929 5741-5745 SO_EXT:0000147 denotes exon
T3930 5753-5757 PR_EXT:000006847 denotes ESG1
T3931 5758-5762 SO_EXT:0000704 denotes gene
T3932 5798-5802 PR_EXT:000006847 denotes ESG1
T3933 5808-5817 SO_EXT:biological_sequence denotes sequences
T3934 5821-5831 GO_SO_EXT:chromosome denotes chromosome
T3935 5879-5882 CHEBI_SO_EXT:DNA denotes DNA
T3936 5908-5912 PR_EXT:000006847 denotes ESG1
T3937 5913-5917 SO_EXT:0000704 denotes gene
T3938 5935-5944 NCBITaxon:2 denotes bacterial
T3939 5935-5966 SO_EXT:0000153 denotes bacterial artificial chromosome
T3940 5956-5966 GO_SO_EXT:chromosome denotes chromosome
T3941 5968-5971 SO_EXT:0000153 denotes BAC
T3942 5973-5976 CHEBI_SO_EXT:DNA denotes DNA
T3943 5995-6001 SO_EXT:0000112 denotes primer
T3944 6041-6045 SO_EXT:0000704 denotes gene
T3945 6055-6066 SO_EXT:0000336 denotes pseudogenes
T3946 6113-6116 SO_EXT:0000153 denotes BAC
T3947 6117-6123 SO_EXT:sequence_cloned_entity denotes clones
T3948 6187-6193 SO_EXT:sequence_cloned_entity denotes clones
T3949 6206-6214 SO_EXT:biological_sequence denotes sequence
T3950 6249-6253 PR_EXT:000006847 denotes ESG1
T3951 6254-6258 SO_EXT:cDNA denotes cDNA
T3952 6296-6303 SO_EXT:0000188 denotes introns
T3953 6325-6331 SO_EXT:0000188 denotes intron
T3954 6332-6341 SO_EXT:biological_sequence denotes sequences
T4017 8609-8613 SO_EXT:0000704 denotes gene
T4018 8662-8673 SO_EXT:0000336 denotes pseudogenes
T4019 8744-8754 SO_EXT:0000336 denotes pseudogene
T6232 8784-8789 NCBITaxon:10088 denotes mouse
T6233 8790-8794 PR_EXT:000006847 denotes ESG1
T6234 8795-8799 SO_EXT:0000704 denotes gene
T6235 8825-8829 PR_EXT:000006847 denotes ESG1
T6236 8834-8841 SO_EXT:sequence_deletion_process denotes deleted
T6237 8846-8850 SO_EXT:0000704 denotes gene
T6238 8865-8878 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T6239 8882-8887 NCBITaxon:10088 denotes mouse
T6240 8888-8896 CL:0002322 denotes ES cells
T6241 8891-8896 CL_GO_EXT:cell denotes cells
T6242 8901-8909 SO_EXT:sequence_substitution_process denotes replaced
T6243 8920-8925 SO_EXT:0000147 denotes exons
T6244 8940-8949 _FRAGMENT denotes fusion of
T6245 8994-8999 SO_EXT:fusion_gene_or_gene_fusion denotes genes
T6246 8954-8962 CHEBI:7507 denotes neomycin
T6247 8978-8993 GO_EXT:0004565 denotes β-galactosidase
T6248 9001-9006 GO_EXT:beta_geo denotes β-geo
T6249 9015-9025 CHEBI:24753 denotes hygromycin
T6250 9036-9040 SO_EXT:0000704 denotes gene
T6251 9058-9075 SO_EXT:0001644 denotes targeting vectors
T6252 9108-9116 CL:0002322 denotes ES cells
T6253 9111-9116 CL_GO_EXT:cell denotes cells
T6254 9155-9162 CL:0002322 denotes ES cell
T6255 9158-9162 CL_GO_EXT:cell denotes cell
T6256 9163-9169 SO_EXT:sequence_cloned_entity denotes clones
T6257 9194-9207 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T6258 9215-9220 GO_EXT:beta_geo denotes β-geo
T6259 9221-9237 SO_EXT:0001644 denotes targeting vector
T6260 9319-9324 SO_EXT:sequence_cloned_entity denotes clone
T6261 9349-9362 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T6262 9375-9391 SO_EXT:0001644 denotes targeting vector
T6301 11108-11113 CL_GO_EXT:cell denotes cells
T6302 11176-11187 UBERON:0000923 denotes germ layers
T6303 11229-11233 PR_EXT:000006847 denotes ESG1
T6304 11257-11269 GO:0017145 denotes self-renewal
T6305 11301-11309 CL:0002322 denotes ES cells
T6306 11304-11309 CL_GO_EXT:cell denotes cells
T6307 11837-11838 SO_EXT:wild_type_entity_or_quality denotes W
T6327 12077-12078 SO_EXT:sequence_nullness_or_absence denotes -
T6328 12079-12087 CL:0002322 denotes ES cells
T6329 12082-12087 CL_GO_EXT:cell denotes cells
T6330 12105-12115 GO:0010467 denotes expression
T6331 12119-12126 CL:0002322 denotes ES cell
T6332 12122-12126 CL_GO_EXT:cell denotes cell
T6333 12127-12133 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T6334 12134-12139 SO_EXT:0000704 denotes genes
T6335 12149-12154 PR_EXT:000010968 denotes Nanog
T6336 12159-12163 PR_EXT:000013044 denotes Oct3
T6337 12181-12185 PR_EXT:000006847 denotes ESG1
T6338 12185-12186 SO_EXT:sequence_nullness_or_absence denotes -
T6339 12187-12188 SO_EXT:sequence_nullness_or_absence denotes -
T6340 12189-12197 CL:0002322 denotes ES cells
T6341 12192-12197 CL_GO_EXT:cell denotes cells
T6342 12219-12223 PR_EXT:000013044 denotes Oct3
T6343 12226-12236 GO:0010467 denotes expression
T6344 12240-12247 CHEBI_PR_EXT:protein denotes protein
T6345 12296-12300 SO_EXT:0000704 denotes gene
T6346 12296-12311 GO:0010467 denotes gene expression
T6347 12350-12358 CL:0002322 denotes ES cells
T6348 12353-12358 CL_GO_EXT:cell denotes cells
T6349 12363-12367 PR_EXT:000006847 denotes ESG1
T6350 12367-12368 SO_EXT:sequence_nullness_or_absence denotes -
T6351 12369-12370 SO_EXT:sequence_nullness_or_absence denotes -
T6352 12371-12379 CL:0002322 denotes ES cells
T6353 12374-12379 CL_GO_EXT:cell denotes cells
T6354 12389-12394 SO_EXT:0000704 denotes genes
T6355 12442-12446 PR_EXT:000006847 denotes ESG1
T6356 12446-12447 SO_EXT:sequence_nullness_or_absence denotes -
T6357 12448-12449 SO_EXT:sequence_nullness_or_absence denotes -
T6358 12450-12455 CL_GO_EXT:cell denotes cells
T6359 12467-12471 PR_EXT:000009450 denotes Krt1
T6360 12475-12478 PR_EXT:000014397 denotes Pem
T6361 12480-12484 PR_EXT:000003172 denotes Ctgf
T6362 12486-12491 PR_EXT:000013428 denotes Ptgs2
T6363 12493-12497 CHEBI_PR_EXT:insulin_like_growth_factor_2 denotes Igf2
T6364 12502-12507 PR_EXT:000000216 denotes Inhba
T6365 12515-12520 SO_EXT:0000704 denotes genes
T6366 12530-12539 GO:0065007 denotes regulated
T6367 12566-12570 PR_EXT:000006847 denotes ESG1
T6368 12578-12582 PR_EXT:000006847 denotes ESG1
T6369 12602-12605 CHEBI_SO_EXT:RNA denotes RNA
T6370 12606-12613 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T6371 12614-12620 SO_EXT:0000417 denotes domain
T6372 12639-12643 CHEBI_SO_EXT:mRNA denotes mRNA
T6373 12653-12658 SO_EXT:0000704 denotes genes
T6401 13451-13452 SO_EXT:sequence_nullness_or_absence denotes -
T6403 13465-13472 NCBITaxon:33208 denotes animals
T6404 13576-13582 UBERON:0000473 denotes testis
T6405 13587-13592 UBERON:0000992 denotes ovary
T6406 13672-13676 PR_EXT:000006847 denotes ESG1
T6407 13701-13706 NCBITaxon:10088 denotes mouse
T6408 13723-13732 CL:0000586 denotes germ cell
T6409 13723-13742 GO:0007281 denotes germ cell formation
T6410 13728-13732 CL_GO_EXT:cell denotes cell
T6411 13762-13770 CL:0002322 denotes ES cells
T6412 13765-13770 CL_GO_EXT:cell denotes cells
T6413 13776-13787 UBERON:0000358 denotes blastocysts
T6414 13816-13820 PR_EXT:000006847 denotes ESG1
T6415 13820-13821 SO_EXT:normal_or_wild_type_or_present denotes +
T6416 13822-13823 SO_EXT:sequence_nullness_or_absence denotes -
T6417 13824-13829 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T6418 13834-13838 PR_EXT:000006847 denotes ESG1
T6419 13838-13839 SO_EXT:sequence_nullness_or_absence denotes -
T6420 13840-13841 SO_EXT:sequence_nullness_or_absence denotes -
T6421 13842-13849 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T6422 13864-13871 CL:0002322 denotes ES cell
T6423 13867-13871 CL_GO_EXT:cell denotes cell
T6424 13895-13901 SO_EXT:sequence_cloned_entity denotes clones
T6425 13907-13911 PR_EXT:000006847 denotes ESG1
T6426 13911-13912 SO_EXT:sequence_nullness_or_absence denotes -
T6427 13913-13914 SO_EXT:sequence_nullness_or_absence denotes -
T6428 13922-13926 PR_EXT:000006847 denotes ESG1
T6429 13927-13931 SO_EXT:sequence_nullness denotes null
T6430 13932-13940 CL:0002322 denotes ES cells
T6431 13935-13940 CL_GO_EXT:cell denotes cells
T6432 13973-13986 GO:0008283 denotes proliferation
T6433 13992-14007 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T6434 14037-14041 PR_EXT:000006847 denotes ESG1
T6435 14060-14068 CL:0002322 denotes ES cells
T6436 14063-14068 CL_GO_EXT:cell denotes cells
T7347 14120-14124 PR_EXT:000006847 denotes ESG1
T7348 14097-14110 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T7349 14144-14149 NCBITaxon:10088 denotes mouse
T7350 14150-14154 SO_EXT:0000704 denotes gene
T7351 14158-14168 GO_SO_EXT:chromosome denotes chromosome
T7352 14175-14182 SO_EXT:sequence_deletion_process denotes deleted
T7353 14200-14213 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T7354 14217-14225 CL:0002322 denotes ES cells
T7355 14220-14225 CL_GO_EXT:cell denotes cells
T7356 14244-14254 GO:0010467 denotes expression
T7357 14258-14271 UBERON:0019248 denotes early embryos
T7358 14273-14283 CL:0000586 denotes germ cells
T7359 14278-14283 CL_GO_EXT:cell denotes cells
T7360 14301-14306 CL_GO_EXT:cell denotes cells
T7361 14335-14339 PR_EXT:000006847 denotes ESG1
T7362 14359-14364 NCBITaxon:10088 denotes mouse
T7363 14378-14387 CL:0000586 denotes germ cell
T7364 14378-14397 GO:0007281 denotes germ cell formation
T7365 14383-14387 CL_GO_EXT:cell denotes cell
T7366 14403-14410 CL:0002322 denotes ES cell
T7367 14406-14410 CL_GO_EXT:cell denotes cell
T7368 14406-14423 GO:0017145 denotes cell self-renewal
T8193 14466-14469 SO_EXT:0000153 denotes BAC
T8194 14470-14476 SO_EXT:sequence_cloned_entity denotes clones
T8195 14492-14497 NCBITaxon:10088 denotes mouse
T8196 14498-14502 PR_EXT:000006847 denotes ESG1
T8197 14503-14507 SO_EXT:0000704 denotes gene
T8198 14520-14529 NCBITaxon:2 denotes bacterial
T8199 14520-14551 SO_EXT:0000153 denotes bacterial artificial chromosome
T8200 14541-14551 GO_SO_EXT:chromosome denotes chromosome
T8201 14553-14556 SO_EXT:0000153 denotes BAC
T8202 14558-14564 SO_EXT:sequence_cloned_entity denotes clones
T8203 14576-14581 NCBITaxon:10088 denotes mouse
T8204 14582-14586 PR_EXT:000006847 denotes ESG1
T8205 14587-14591 SO_EXT:0000704 denotes gene
T8206 14629-14634 NCBITaxon:10088 denotes mouse
T8207 14635-14638 SO_EXT:0000153 denotes BAC
T8208 14647-14650 CHEBI_SO_EXT:DNA denotes DNA
T8209 14667-14675 OBI_SO_EXT:gene_or_genetic_investigation_or_genetic_process denotes Genetics
T8210 14687-14691 PR_EXT:000006847 denotes pH34
T8211 14730-14734 PR_EXT:000006847 denotes pH34
T8212 14769-14776 SO_EXT:0000112 denotes primers
T8213 14788-14806 GO_EXT:0015666 denotes restriction enzyme
T8214 14800-14806 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T8215 14857-14860 SO_EXT:0000153 denotes BAC
T8216 14861-14867 SO_EXT:sequence_cloned_entity denotes clones
T8217 14896-14900 PR_EXT:000006847 denotes pH34
T8218 14946-14950 PR_EXT:000006847 denotes pH34
T8219 14985-14989 PR_EXT:000006847 denotes pH34
T8220 15035-15039 PR_EXT:000006847 denotes pH34
T8221 15082-15086 PR_EXT:000006847 denotes pH34
T8222 15126-15132 CHEBI_SO_EXT:molecular_probe denotes probes
T8223 15134-15147 GO:0097617 denotes Hybridization
T8224 15159-15165 CHEBI_SO_EXT:molecular_probe denotes probes
T8225 15203-15207 PR_EXT:000006847 denotes ESG1
T8226 15208-15212 SO_EXT:0000704 denotes gene
T8227 15216-15227 SO_EXT:0000336 denotes pseudogenes
T8228 15267-15272 NCBITaxon:10088 denotes mouse
T8229 15273-15277 PR_EXT:000006847 denotes ESG1
T8230 15278-15282 SO_EXT:0000704 denotes gene
T8231 15291-15309 SO_EXT:0001417 denotes 3' flanking region
T8232 15331-15333 SO_EXT:0000028 denotes bp
T8233 15370-15376 SO_EXT:0000440 denotes vector
T8234 15391-15398 PR_EXT:P43870 denotes HindIII
T8235 15436-15442 SO_EXT:0000440 denotes vector
T8236 15453-15459 SO_EXT:sequence_cloning_process denotes cloned
T8237 15515-15519 PR_EXT:000006847 denotes ESG1
T8238 15520-15530 SO_EXT:0000336 denotes pseudogene
T8239 15539-15557 SO_EXT:0001417 denotes 3' flanking region
T8240 15565-15567 SO_EXT:0000028 denotes bp
T8241 15591-15597 SO_EXT:sequence_cloning_process denotes cloned
T8242 15622-15627 PR_EXT:P23940 denotes BamHI
T8243 15656-15662 SO_EXT:0000440 denotes vector
T8244 15673-15679 SO_EXT:sequence_cloning_process denotes cloned
T8245 15720-15728 SO_EXT:biological_sequence denotes sequence
T8246 15744-15763 SO_EXT:0001416 denotes 5' flanking regions
T8247 15771-15775 PR_EXT:000006847 denotes ESG1
T8248 15776-15780 SO_EXT:0000704 denotes gene
T8249 15797-15808 SO_EXT:0000336 denotes pseudogenes
T8250 15858-15862 PR_EXT:000006847 denotes pH34
T8251 15910-15914 PR_EXT:000006847 denotes pH34
T8252 15920-15927 SO_EXT:0000112 denotes primers
T8253 15943-15951 SO_EXT:biological_sequence denotes sequence
T8254 15956-15962 SO_EXT:sequence_cloning_process denotes cloned
T8255 16007-16009 SO_EXT:0000028 denotes bp
T8256 16116-16122 SO_EXT:sequence_cloning_process denotes cloned
T8257 16190-16208 SO_EXT:0001416 denotes 5' flanking region
T8258 16216-16220 PR_EXT:000006847 denotes ESG1
T8259 16221-16225 SO_EXT:0000704 denotes gene
T8260 16233-16235 SO_EXT:0000028 denotes bp
T8261 16291-16297 SO_EXT:sequence_cloning_process denotes cloned
T8262 16368-16376 SO:0000357 denotes flanking
T8263 16381-16391 SO_EXT:0000336 denotes pseudogene
T9129 16410-16414 PR_EXT:000006847 denotes ESG1
T9130 16415-16432 SO_EXT:0001644 denotes targeting vectors
T9131 16436-16444 SO_EXT:sequence_substitution_process denotes replaced
T9132 16456-16460 PR_EXT:000006847 denotes ESG1
T9133 16461-16466 SO_EXT:0000147 denotes exons
T9134 16476-16493 SO_EXT:0001644 denotes targeting vectors
T9135 16515-16519 SO_EXT:0000243 denotes IRES
T9136 16520-16525 GO_EXT:beta_geo denotes β-geo
T9137 16526-16534 SO_EXT:0005853 denotes cassette
T9138 16546-16550 SO_EXT:0000243 denotes IRES
T9139 16556-16564 SO_EXT:0005853 denotes cassette
T9140 16568-16576 SO_EXT:0000167 denotes promoter
T9141 16623-16625 SO_EXT:0000028 denotes bp
T9142 16660-16664 PR_EXT:000006847 denotes pH34
T9143 16718-16722 PR_EXT:000006847 denotes pH34
T9144 16776-16783 SO_EXT:0000112 denotes primers
T9145 16802-16804 SO_EXT:0000028 denotes bp
T9146 16830-16834 PR_EXT:000006847 denotes pH34
T9147 16888-16892 PR_EXT:000006847 denotes pH34
T9148 16942-16949 SO_EXT:0000112 denotes primers
T9149 16955-16959 SO_EXT:0000243 denotes IRES
T9150 16960-16965 GO_EXT:beta_geo denotes β-geo
T9151 16969-16973 SO_EXT:0000243 denotes IRES
T9152 16979-16988 SO_EXT:0005853 denotes cassettes
T9153 16994-17001 GO:0006266 denotes ligated
T9154 17051-17056 CHEBI_EXT:27026 denotes toxin
T9155 17059-17067 SO_EXT:0005853 denotes cassette
T9156 17079-17089 SO_EXT:sequence_downstreamness denotes downstream
T9157 17111-17124 SO_EXT:sequence_linearization denotes linearization
T9158 17143-17160 SO_EXT:0001644 denotes targeting vectors
T9159 17200-17208 CL:0002322 denotes ES cells
T9160 17203-17208 CL_GO_EXT:cell denotes cells
T9161 17222-17226 SO_EXT:0000704 denotes Gene
T9162 17244-17255 GO:0009294 denotes Transfected
T9163 17256-17261 CL_GO_EXT:cell denotes cells
T9164 17291-17295 CHEBI:42768 denotes G418
T9165 17309-17321 CHEBI:16976 denotes hygromycin B
T9166 17337-17348 SO_EXT:genomic_DNA denotes Genomic DNA
T9167 17345-17348 CHEBI_SO_EXT:DNA denotes DNA
T9168 17354-17358 CHEBI:42768 denotes G418
T9169 17363-17375 CHEBI:16976 denotes hygromycin B
T9170 17423-17436 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T9797 17499-17512 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T9798 17513-17521 CL:0002322 denotes ES cells
T9799 17516-17521 CL_GO_EXT:cell denotes cells
T9800 17522-17533 SO_EXT:genomic_DNA denotes genomic DNA
T9801 17530-17533 CHEBI_SO_EXT:DNA denotes DNA
T9802 17563-17567 CL_GO_EXT:cell denotes Cell
T9803 17563-17573 GO:0019835 denotes Cell Lysis
T9804 17574-17582 CHEBI:75958 denotes Solution
T9805 17632-17643 SO_EXT:genomic_DNA denotes genomic DNA
T9806 17640-17643 CHEBI_SO_EXT:DNA denotes DNA
T9807 17700-17707 CHEBI:2511 denotes agarose
T9808 17716-17727 GO_EXT:biological_movement_or_translocation_process denotes transferred
T9809 17733-17738 CHEBI:25614 denotes nylon
T9810 17773-17775 SO_EXT:0000028 denotes bp
T9811 17779-17784 CHEBI_SO_EXT:molecular_probe denotes probe
T9812 17895-17902 SO_EXT:0000112 denotes primers
T9813 17908-17913 CHEBI_SO_EXT:molecular_probe denotes probe
T9814 17927-17934 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T9815 17942-17944 SO_EXT:0000028 denotes bp
T9816 17959-17968 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9817 17982-17984 SO_EXT:0000028 denotes bp
T9818 17999-18004 GO_EXT:beta_geo denotes β-geo
T9819 18022-18024 SO_EXT:0000028 denotes bp
T9820 18051-18062 SO_EXT:genomic_DNA denotes Genomic DNA
T9821 18059-18062 CHEBI_SO_EXT:DNA denotes DNA
T9822 18130-18132 SO_EXT:0000028 denotes bp
T9823 18136-18141 CHEBI_SO_EXT:molecular_probe denotes probe
T9824 18260-18267 SO_EXT:0000112 denotes primers
T9825 18273-18278 CHEBI_SO_EXT:molecular_probe denotes probe
T9826 18279-18289 GO:0097617 denotes hybridized
T9827 18302-18304 SO_EXT:0000028 denotes bp
T9828 18319-18328 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9829 18344-18346 SO_EXT:0000028 denotes bp
T9830 18361-18366 GO_EXT:beta_geo denotes β-geo
T9831 18385-18387 SO_EXT:0000028 denotes bp
T10380 18415-18428 _FRAGMENT denotes Generation of
T10381 18450-18460 GO_EXT:antibody_or_immunoglobulin_biosynthesis_or_production denotes antibodies
T10382 18434-18438 PR_EXT:000006847 denotes ESG1
T10383 18450-18460 GO:0042571 denotes antibodies
T10384 18465-18480 SO_EXT:coding_sequence denotes coding sequence
T10385 18484-18488 PR_EXT:000006847 denotes Esg1
T10386 18519-18523 PR_EXT:000006847 denotes pH34
T10387 18576-18580 PR_EXT:000006847 denotes pH34
T10388 18629-18636 SO_EXT:0000112 denotes primers
T10389 18657-18661 PR_EXT:000006847 denotes pH34
T10390 18677-18688 SO_EXT:0000006 denotes PCR product
T10391 18737-18741 PR_EXT:000006847 denotes pH34
T10392 18789-18802 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T10393 18840-18850 GO:0010467 denotes expression
T10394 18851-18857 SO_EXT:0000440 denotes vector
T10395 18866-18870 PR_EXT:000006847 denotes pH34
T10396 18884-18891 NCBITaxon:562 denotes E. coli
T10397 18906-18917 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T10398 18918-18925 CHEBI_PR_EXT:protein denotes protein
T10399 18918-18936 GO:0006412 denotes protein production
T10400 18990-18999 CHEBI_SO_EXT:histidine denotes Histidine
T10401 19000-19006 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T10402 19007-19011 PR_EXT:000006847 denotes ESG1
T10403 19031-19033 CHEBI_EXT:nickel denotes Ni
T10404 19034-19055 CHEBI:44557 denotes nitrilotriacetic acid
T10405 19056-19063 CHEBI:2511 denotes agarose
T10406 19079-19089 GO_EXT:macromolecule_denaturation denotes denaturing
T10407 19124-19128 CHEBI:16199 denotes urea
T10408 19157-19161 CHEBI:16199 denotes urea
T10409 19167-19178 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T10410 19179-19187 CHEBI_PR_EXT:protein denotes proteins
T10411 19225-19232 NCBITaxon:9986 denotes rabbits
T10412 19250-19254 PR_EXT:000006847 denotes ESG1
T10413 19266-19276 GO:0042571 denotes antibodies
T10831 19313-19320 CL:0002322 denotes ES cell
T10832 19316-19320 CL_GO_EXT:cell denotes cell
T10833 19351-19359 CL_GO_EXT:cell denotes cellular
T10834 19360-19368 CHEBI_PR_EXT:protein denotes proteins
T10835 19387-19409 CHEBI:8984 denotes sodium dodecyl sulfate
T10836 19411-19414 CHEBI:8984 denotes SDS
T10837 19420-19434 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T10838 19444-19455 GO_EXT:biological_movement_or_translocation_process denotes transferred
T10839 19459-19473 CHEBI:53325 denotes nitrocellulose
T10840 19532-19536 PR_EXT:000006847 denotes ESG1
T10841 19560-19564 PR_EXT:000013044 denotes Oct3
T10842 19607-19611 PR_EXT:000005257 denotes CDK4
T10843 19682-19692 GO:0042571 denotes antibodies
T10844 19694-19705 NCBITaxon:3704 denotes Horseradish
T10845 19706-19716 GO_EXT:0004601 denotes peroxidase
T10846 19717-19727 MOP:0000779 denotes conjugated
T10847 19733-19739 NCBITaxon:9986 denotes rabbit
T10848 19749-19754 NCBITaxon:10088 denotes mouse
T10849 19755-19770 GO:0019814 denotes immunoglobulins
T10850 19780-19784 CL_GO_EXT:cell denotes Cell
T10851 19785-19794 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes Signaling
T10852 19816-19824 GO:0042571 denotes antibody
T10853 19825-19832 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T10854 19848-19853 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bound
T10855 19854-19862 GO:0042571 denotes antibody
T11434 19936-19940 PR_EXT:000006847 denotes ESG1
T11435 19951-19959 CL:0002322 denotes ES cells
T11436 19954-19959 CL_GO_EXT:cell denotes cells
T11437 19965-19976 UBERON:0000358 denotes blastocysts
T11438 19977-19981 PR_EXT:000006847 denotes Esg1
T11439 19981-19982 SO_EXT:normal_or_wild_type_or_present denotes +
T11440 19983-19984 SO_EXT:sequence_nullness_or_absence denotes -
T11441 19987-19991 PR_EXT:000006847 denotes ESG1
T11442 19991-19992 SO_EXT:sequence_nullness_or_absence denotes -
T11443 19993-19994 SO_EXT:sequence_nullness_or_absence denotes -
T11444 19995-20001 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T11445 20002-20008 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T11446 20009-20013 NCBITaxon:10088 denotes mice
T11447 20033-20042 CHEBI:41774 denotes Tamoxifen
T11448 20055-20067 CHEBI:6716 denotes Depo-provera
T11449 20078-20089 UBERON_EXT:zone_of_skin_or_skin_of_body denotes cutaneously
T11450 20110-20119 GO:0007565 denotes pregnancy
T11451 20138-20145 UBERON:0000922 denotes embryos
T11452 20182-20188 UBERON:0000995 denotes uterus
T11453 20216-20221 CL_GO_EXT:cell denotes cells
T11454 20313-20319 NCBITaxon:27592 denotes Bovine
T11455 20320-20325 UBERON:0001977 denotes Serum
T11456 20358-20369 CHEBI_SO_EXT:amino_acid denotes Amino Acids
T11457 20389-20400 CHEBI_EXT:L_glutamine denotes L-glutamine
T11458 20391-20400 CHEBI_SO_EXT:glutamine denotes glutamine
T11459 20423-20433 CHEBI:17334 denotes Penicillin
T11460 20434-20446 CHEBI:17076 denotes Streptomysin
T11461 20473-20490 CHEBI:41218 denotes 2-mercaptoethanol
T11462 20608-20615 PR_EXT:000027795 denotes trypsin
T11463 20639-20643 CL_GO_EXT:cell denotes cell
T11464 20639-20648 UBERON_EXT:cell_clump_or_cluster_or_group_or_mass_or_population denotes cell mass
T11465 20760-20767 PR_EXT:000027795 denotes trypsin
T11466 20773-20778 CL_GO_EXT:cell denotes cells
T11467 20791-20801 GO_EXT:biological_movement_or_translocation_process denotes transfered
T11468 20866-20873 _FRAGMENT denotes primary
T11469 20877-20881 CL:0000001 denotes cell
T11470 20874-20881 CL:0002322 denotes ES cell
T11471 20877-20881 CL_GO_EXT:cell denotes cell
T11472 20971-20975 CL_GO_EXT:cell denotes cell
T11473 20971-20982 UBERON:0000119 denotes cell layers
T11474 20996-21001 CL_GO_EXT:cell denotes cells
T11475 21019-21033 GO_EXT:trypsinization denotes trypsinization
T11866 21076-21079 CHEBI_SO_EXT:RNA denotes RNA
T11867 21085-21094 SO_EXT:wild_type_entity_or_quality denotes wild-type
T11868 21095-21103 CL:0002322 denotes ES cells
T11869 21098-21103 CL_GO_EXT:cell denotes cells
T11870 21108-21112 PR_EXT:000006847 denotes ESG1
T11871 21112-21113 SO_EXT:sequence_nullness_or_absence denotes -
T11872 21114-21115 SO_EXT:sequence_nullness_or_absence denotes -
T11873 21116-21124 CL:0002322 denotes ES cells
T11874 21119-21124 CL_GO_EXT:cell denotes cells
T11875 21129-21136 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T11876 21142-21145 CHEBI:37987 denotes Cy3
T11877 21150-21153 CHEBI:37989 denotes Cy5
T11878 21186-21196 GO:0097617 denotes hybridized
T11879 21202-21207 NCBITaxon:10088 denotes Mouse
T11880 21356-21369 GO:0097617 denotes Hybridization
T11881 21404-21410 SO_EXT:sequence_cloned_entity denotes clones
T11882 21465-21473 OBI_SO_EXT:gene_or_genetic_investigation_or_genetic_process denotes Genetics
T12044 5103-5107 PR_EXT:000006847 denotes ESG1
T12045 5107-5118 SO_EXT:0000336 denotes pseudogenes
T12046 5151-5156 NCBITaxon:10088 denotes mouse
T12047 5157-5164 SO_EXT:0001026 denotes genomic
T12048 5176-5188 SO_EXT:sequence_substitution_entity_or_process denotes Substitution
T12049 5189-5198 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12050 5229-5235 SO_EXT:0000188 denotes Intron
T12051 5241-5244 SO_EXT:0000730 denotes gap
T12052 5245-5254 SO_EXT:biological_sequence denotes sequences
T12053 5285-5296 GO_SO_EXT:chromosome denotes Chromosomal
T12054 5297-5310 GO_PATO_EXT:biological_localization_process_or_quality denotes localizations
T12250 6780-6783 SO_EXT:0000153 denotes BAC
T12251 6784-6790 SO_EXT:sequence_cloned_entity denotes clones
T12252 6806-6810 PR_EXT:000006847 denotes ESG1
T12253 6811-6815 SO_EXT:0000704 denotes gene
T12254 6822-6833 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T12255 6834-6844 SO_EXT:0000336 denotes pseudogene
T12256 6846-6848 SO_EXT:0000336 denotes PS
T12257 6854-6866 GO_PATO_EXT:biological_localization_process_or_quality denotes Localization
T12258 6874-6878 SO_EXT:0000704 denotes gene
T12259 6883-6885 SO_EXT:0000336 denotes PS
T12260 6889-6899 GO_SO_EXT:chromosome denotes chromosome
T12261 6906-6914 SO_EXT:biological_sequence denotes Sequence
T12262 6933-6937 SO_EXT:0000704 denotes gene
T12263 6942-6944 SO_EXT:0000336 denotes PS
T12264 6969-6979 SO:0000857 denotes homologous
T12265 6991-6995 CHEBI_SO_EXT:base denotes base
T12266 6991-7001 SO_EXT:0000028 denotes base pairs
T12267 7002-7010 SO_EXT:sequence_upstreamness denotes upstream
T12268 7024-7028 SO_EXT:0000147 denotes exon
T12269 7036-7038 SO_EXT:0000028 denotes bp
T12270 7039-7049 SO_EXT:sequence_downstreamness denotes downstream
T12271 7063-7067 SO_EXT:0000147 denotes exon
T12272 7069-7081 SO_EXT:sequence_substitution_entity_or_process denotes Substitution
T12273 7082-7091 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12274 7126-7135 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T12522 7180-7188 SO_EXT:0000167 denotes Promoter
T12523 7189-7197 SO_EXT:0000165 denotes enhancer
T12524 7214-7218 PR_EXT:000006847 denotes ESG1
T12525 7219-7223 SO_EXT:0000704 denotes gene
T12526 7228-7238 SO_EXT:0000336 denotes pseudogene
T12527 7240-7243 CHEBI_SO_EXT:DNA denotes DNA
T12528 7261-7263 SO_EXT:0000028 denotes bp
T12529 7282-7301 SO_EXT:0001416 denotes 5' flanking regions
T12530 7309-7313 SO_EXT:0000704 denotes gene
T12531 7318-7320 SO_EXT:0000336 denotes PS
T12532 7326-7337 GO_EXT:biological_movement_or_translocation_process denotes transferred
T12533 7343-7353 GO_EXT:0045289 denotes luciferase
T12534 7363-7371 SO_EXT:0000155 denotes plasmids
T12535 7400-7405 SO_EXT:0000704 denotes genes
T12536 7413-7427 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T12537 7428-7436 CL:0002322 denotes ES cells
T12538 7431-7436 CL_GO_EXT:cell denotes cells
T12539 7453-7466 CHEBI:26536 denotes retinoic acid
T12540 7475-7483 CL:0002322 denotes ES cells
T12541 7478-7483 CL_GO_EXT:cell denotes cells
T12542 7511-7516 CL_GO_EXT:cell denotes cells
T13029 9430-9435 NCBITaxon:10088 denotes mouse
T13030 9436-9440 PR_EXT:000006847 denotes ESG1
T13031 9441-9445 SO_EXT:0000704 denotes gene
T13032 9519-9536 SO_EXT:0000409 denotes Recognition sites
T13033 9622-9626 SO_EXT:0000704 denotes gene
T13034 9627-9635 SO_EXT:sequence_coding_function denotes encoding
T13035 9647-9652 CHEBI_EXT:27026 denotes toxin
T13036 9665-9673 SO_EXT:sequence_insertion_process denotes inserted
T13037 9695-9712 SO_EXT:0001644 denotes targeting vectors
T13038 9795-9808 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T13039 9810-9812 SO_EXT:wild_type_entity_or_quality denotes WT
T13040 9814-9823 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13041 9824-9832 CL:0002322 denotes ES cells
T13042 9827-9832 CL_GO_EXT:cell denotes cells
T13043 9837-9842 GO_EXT:beta_geo denotes β-geo
T13044 9843-9844 SO_EXT:normal_or_wild_type_or_present denotes +
T13045 9845-9846 SO_EXT:sequence_nullness_or_absence denotes -
T13046 9847-9855 CL:0002322 denotes ES cells
T13047 9850-9855 CL_GO_EXT:cell denotes cells
T13048 9865-9866 SO_EXT:normal_or_wild_type_or_present denotes +
T13049 9867-9868 SO_EXT:sequence_nullness_or_absence denotes -
T13050 9869-9877 CL:0002322 denotes ES cells
T13051 9872-9877 CL_GO_EXT:cell denotes cells
T13052 9879-9880 SO_EXT:sequence_nullness_or_absence denotes -
T13053 9881-9882 SO_EXT:sequence_nullness_or_absence denotes -
T13054 9884-9888 PR_EXT:000006847 denotes ESG1
T13055 9889-9893 SO_EXT:sequence_nullness denotes null
T13056 9894-9902 CL:0002322 denotes ES cells
T13057 9897-9902 CL_GO_EXT:cell denotes cells
T13058 9921-9926 SO_EXT:sequence_cloned_entity denotes clone
T13059 9998-10007 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13060 10008-10016 CL:0002322 denotes ES cells
T13061 10011-10016 CL_GO_EXT:cell denotes cells
T13062 10018-10020 SO_EXT:wild_type_entity_or_quality denotes WT
T13063 10023-10027 PR_EXT:000006847 denotes ESG1
T13064 10028-10032 SO_EXT:sequence_nullness denotes null
T13065 10033-10041 CL:0002322 denotes ES cells
T13066 10036-10041 CL_GO_EXT:cell denotes cells
T13067 10043-10044 SO_EXT:sequence_nullness_or_absence denotes -
T13068 10045-10046 SO_EXT:sequence_nullness_or_absence denotes -
T13069 10054-10060 SO_EXT:sequence_cloned_entity denotes clones
T13070 10079-10087 CL:0002322 denotes ES cells
T13071 10082-10087 CL_GO_EXT:cell denotes cells
T13072 10089-10090 SO_EXT:normal_or_wild_type_or_present denotes +
T13073 10091-10092 SO_EXT:sequence_nullness_or_absence denotes -
T13074 10196-10199 CHEBI_SO_EXT:RNA denotes RNA
T13075 10201-10217 CHEBI:4883 denotes ethidium bromide
T13076 10230-10239 GO:0005840 denotes ribosomal
T13077 10230-10243 CHEBI_SO_EXT:rRNA denotes ribosomal RNA
T13551 11333-11337 PR_EXT:000006847 denotes ESG1
T13552 11338-11342 SO_EXT:sequence_nullness denotes null
T13553 11343-11351 CL:0002322 denotes ES cells
T13554 11346-11351 CL_GO_EXT:cell denotes cells
T13555 11374-11378 PR_EXT:000006847 denotes ESG1
T13556 11379-11383 SO_EXT:sequence_nullness denotes null
T13557 11384-11391 CL:0002322 denotes ES cell
T13558 11387-11391 CL_GO_EXT:cell denotes cell
T13559 11401-11406 GO:0040007 denotes grown
T13560 11421-11426 CL_GO_EXT:cell denotes cells
T13561 11431-11437 GO_EXT:biological_growth_entity_or_process denotes Growth
T13562 11447-11456 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13563 11458-11460 SO_EXT:wild_type_entity_or_quality denotes WT
T13564 11463-11467 PR_EXT:000006847 denotes ESG1
T13565 11468-11472 SO_EXT:sequence_nullness denotes null
T13566 11474-11475 SO_EXT:sequence_nullness_or_absence denotes -
T13567 11476-11477 SO_EXT:sequence_nullness_or_absence denotes -
T13568 11497-11498 SO_EXT:normal_or_wild_type_or_present denotes +
T13569 11499-11500 SO_EXT:sequence_nullness_or_absence denotes -
T13570 11502-11510 CL:0002322 denotes ES cells
T13571 11505-11510 CL_GO_EXT:cell denotes cells
T13572 11517-11522 SO_EXT:sequence_cloned_entity denotes clone
T13573 11532-11537 CL_GO_EXT:cell denotes cells
T13574 11574-11578 CL_GO_EXT:cell denotes Cell
T13575 11655-11656 SO_EXT:normal_or_wild_type_or_present denotes +
T13576 11657-11658 SO_EXT:sequence_nullness_or_absence denotes -
T13577 11663-11664 SO_EXT:sequence_nullness_or_absence denotes -
T13578 11665-11666 SO_EXT:sequence_nullness_or_absence denotes -
T13579 11667-11672 CL_GO_EXT:cell denotes cells
T13580 11740-11746 SO_EXT:sequence_cloned_entity denotes clones
T13581 11786-11790 PR_EXT:000006847 denotes ESG1
T13582 11791-11795 SO_EXT:sequence_nullness denotes null
T13583 11796-11804 CL:0002322 denotes ES cells
T13584 11799-11804 CL_GO_EXT:cell denotes cells
T13585 11806-11817 CHEBI:51686 denotes hematoxylin
T13586 11820-11825 CHEBI_EXT:eosin denotes eosin
T13928 12728-12732 SO_EXT:0000704 denotes Gene
T13929 12728-12743 GO:0010467 denotes Gene expression
T13930 12756-12760 PR_EXT:000006847 denotes ESG1
T13931 12761-12765 SO_EXT:sequence_nullness denotes null
T13932 12766-12774 CL:0002322 denotes ES cells
T13933 12769-12774 CL_GO_EXT:cell denotes cells
T13934 12779-12782 CHEBI_SO_EXT:DNA denotes DNA
T13935 12810-12813 CHEBI_SO_EXT:RNA denotes RNA
T13936 12819-12828 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13937 12829-12837 CL:0002322 denotes ES cells
T13938 12832-12837 CL_GO_EXT:cell denotes cells
T13939 12842-12846 PR_EXT:000006847 denotes ESG1
T13940 12847-12851 SO_EXT:sequence_nullness denotes null
T13941 12852-12860 CL:0002322 denotes ES cells
T13942 12855-12860 CL_GO_EXT:cell denotes cells
T13943 12866-12873 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T13944 12879-12882 CHEBI:37987 denotes Cy3
T13945 12887-12890 CHEBI:37989 denotes Cy5
T13946 12919-12929 GO:0097617 denotes hybridized
T13947 12941-12946 NCBITaxon:10088 denotes Mouse
T13948 13006-13012 SO_EXT:sequence_cloned_entity denotes clones
T13949 13050-13054 CL_GO_EXT:cell denotes Cell
T13950 13068-13072 PR_EXT:000006847 denotes ESG1
T13951 13072-13073 SO_EXT:normal_or_wild_type_or_present denotes +
T13952 13074-13075 SO_EXT:sequence_nullness_or_absence denotes -
T13953 13080-13084 PR_EXT:000006847 denotes ESG1
T13954 13084-13085 SO_EXT:sequence_nullness_or_absence denotes -
T13955 13086-13087 SO_EXT:sequence_nullness_or_absence denotes -
T13956 13088-13093 CL_GO_EXT:cell denotes cells
T13957 13112-13122 GO:0010467 denotes expression
T13958 13126-13130 PR_EXT:000006847 denotes ESG1
T13959 13132-13136 PR_EXT:000013044 denotes Oct3
T13960 13143-13147 PR_EXT:000005257 denotes CDK4
T3969 6759-6763 PR_EXT:000006847 denotes ESG1
T3970 6764-6768 SO_EXT:0000704 denotes gene
T3971 7648-7651 SO_EXT:0000153 denotes BAC
T3992 8064-8074 SO_EXT:0000028 denotes base pairs
T3993 8082-8101 SO_EXT:0001417 denotes 3' flanking regions
T3882 4298-4302 SO_EXT:0000704 denotes gene
T3883 4307-4317 SO_EXT:0000336 denotes psedogenes
T3884 4335-4346 GO_SO_EXT:chromosome denotes chromosomal
T3885 4347-4360 GO_PATO_EXT:biological_localization_process_or_quality denotes localizations
T3886 4368-4373 NCBITaxon:10088 denotes mouse
T3887 4374-4378 PR_EXT:000006847 denotes ESG1
T3888 4379-4383 SO_EXT:0000704 denotes gene
T3889 4388-4399 SO_EXT:0000336 denotes pseudogenes
T3890 4438-4443 NCBITaxon:10088 denotes mouse
T3891 4444-4451 SO_EXT:0001026 denotes genomic
T3892 4470-4474 PR_EXT:000006847 denotes ESG1
T3893 4475-4479 SO_EXT:cDNA denotes cDNA
T3955 6408-6429 SO_EXT:0001421 denotes exon-intron junctions
T3956 6440-6458 SO_EXT:0001416 denotes 5' flanking region
T3957 6467-6470 CHEBI_SO_EXT:DNA denotes DNA
T3958 6497-6505 SO_EXT:0000167 denotes promoter
T3959 6506-6514 SO_EXT:0000165 denotes enhancer
T3960 6527-6537 GO_EXT:0045289 denotes luciferase
T3961 6559-6573 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T3962 6574-6582 CL:0002322 denotes ES cells
T3963 6577-6582 CL_GO_EXT:cell denotes cells
T3964 6595-6608 CL:0002371 denotes somatic cells
T3965 6603-6608 CL_GO_EXT:cell denotes cells
T3966 6686-6701 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T3967 6705-6718 CHEBI:26536 denotes retinoic acid
T3968 6743-6751 SO_EXT:biological_sequence denotes sequence
T3972 7652-7658 SO_EXT:sequence_cloned_entity denotes clones
T3973 7677-7681 PR_EXT:000006847 denotes ESG1
T3974 7687-7695 SO_EXT:biological_sequence denotes sequence
T3975 7717-7726 SO_EXT:biological_sequence denotes sequences
T3976 7747-7749 SO_EXT:0000028 denotes bp
T3977 7750-7769 SO_EXT:0000605 denotes intergenic sequence
T3978 7813-7817 PR_EXT:000006847 denotes ESG1
T3979 7823-7831 SO_EXT:biological_sequence denotes sequence
T3980 7875-7880 SO_EXT:0000147 denotes exons
T3981 7885-7892 SO_EXT:0000188 denotes introns
T3982 7900-7904 PR_EXT:000006847 denotes ESG1
T3983 7905-7909 SO_EXT:0000704 denotes gene
T3984 7916-7924 SO_EXT:biological_sequence denotes sequence
T3985 7954-7964 CHEBI_SO_EXT:nucleotide denotes nucleotide
T3986 7965-7978 SO_EXT:sequence_substitution_entity_or_process denotes substitutions
T3987 7993-7998 SO_EXT:0000147 denotes exons
T3988 8007-8017 CHEBI_SO_EXT:nucleotide denotes nucleotide
T3989 8018-8027 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T3990 8031-8035 SO_EXT:0000147 denotes exon
T3991 8064-8068 CHEBI_SO_EXT:base denotes base
T3994 8107-8116 SO_EXT:biological_conservation_process_or_quality denotes conserved
T3995 8129-8133 PR_EXT:000006847 denotes ESG1
T3996 8134-8138 SO_EXT:0000704 denotes gene
T3997 8147-8157 SO_EXT:0000336 denotes pseudogene
T3998 8169-8173 CHEBI_SO_EXT:base denotes base
T3999 8169-8179 SO_EXT:0000028 denotes base pairs
T4000 8187-8205 SO_EXT:0001416 denotes 5' flanking region
T4001 8227-8245 SO_EXT:0001416 denotes 5' flanking region
T4002 8251-8253 SO_EXT:0000028 denotes bp
T4003 8275-8283 SO_EXT:0000167 denotes promoter
T4004 8284-8292 SO_EXT:0000165 denotes enhancer
T4005 8305-8315 GO_EXT:0045289 denotes luciferase
T4006 8374-8382 SO_EXT:biological_sequence denotes sequence
T4007 8386-8397 GO_EXT:transcription denotes transcribed
T4008 8401-8411 GO:0006412 denotes translated
T4009 8430-8437 CHEBI_PR_EXT:protein denotes protein
T4010 8444-8452 SO_EXT:biological_sequence denotes sequence
T4011 8473-8484 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T4012 8485-8495 SO_EXT:0000336 denotes pseudogene
T4013 8547-8558 SO_EXT:0000336 denotes pseudogenes
T4014 8572-8576 SO_EXT:0000147 denotes exon
T4015 8577-8583 SO_EXT:0000188 denotes intron
T4016 8604-8608 PR_EXT:000006847 denotes ESG1
T6263 10296-10304 CL:0002322 denotes ES cells
T6264 10299-10304 CL_GO_EXT:cell denotes cells
T6265 10324-10329 GO_EXT:beta_geo denotes β-geo
T6266 10330-10336 SO_EXT:0000440 denotes vector
T6267 10360-10368 CL:0002322 denotes ES cells
T6268 10363-10368 CL_GO_EXT:cell denotes cells
T6269 10377-10381 CHEBI:42768 denotes G418
T6270 10432-10436 PR_EXT:000006847 denotes ESG1
T6271 10437-10445 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T6272 10512-10516 PR_EXT:000006847 denotes ESG1
T6273 10526-10531 CL_GO_EXT:cell denotes cells
T6274 10551-10557 SO_EXT:sequence_cloned_entity denotes clones
T6275 10563-10568 GO_EXT:beta_geo denotes β-geo
T6276 10569-10575 SO_EXT:0000440 denotes vector
T6277 10580-10588 SO_EXT:sequence_substitution_process denotes replaced
T6278 10598-10604 SO_EXT:0000440 denotes vector
T6279 10620-10625 CL_GO_EXT:cell denotes cells
T6280 10669-10675 SO_EXT:sequence_cloned_entity denotes clones
T6281 10681-10686 GO_EXT:beta_geo denotes β-geo
T6282 10687-10693 SO_EXT:0000440 denotes vector
T6283 10734-10738 PR_EXT:000006847 denotes ESG1
T6284 10738-10739 SO_EXT:sequence_nullness_or_absence denotes -
T6285 10740-10741 SO_EXT:sequence_nullness_or_absence denotes -
T6286 10742-10750 CL:0002322 denotes ES cells
T6287 10745-10750 CL_GO_EXT:cell denotes cells
T6288 10798-10803 CL_GO_EXT:cell denotes cells
T6289 10809-10821 GO:0008283 denotes proliferated
T6290 10885-10894 SO_EXT:wild_type_entity_or_quality denotes wild-type
T6291 10896-10901 CL_GO_EXT:cell denotes cells
T6292 10915-10919 PR_EXT:000006847 denotes ESG1
T6293 10919-10920 SO_EXT:sequence_nullness_or_absence denotes -
T6294 10921-10922 SO_EXT:sequence_nullness_or_absence denotes -
T6295 10923-10928 CL_GO_EXT:cell denotes cells
T6296 10929-10943 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T6297 10974-11000 PR_EXT:000009799 denotes leukemia inhibitory factor
T6298 11016-11029 CHEBI:26536 denotes retinoic acid
T6299 11081-11087 UBERON:0000180 denotes flanks
T6300 11096-11100 NCBITaxon:10088 denotes mice
T6308 11840-11841 CL:0002322 denotes e
T6309 11840-11841 CL_GO_EXT:cell denotes e
T6310 11841-11842 CHEBI:51686 denotes x
T6311 11853-11857 SO_EXT:0000704 denotes gene
T6312 11853-11868 GO:0010467 denotes gene expression
T6313 11881-11885 PR_EXT:000006847 denotes ESG1
T6314 11885-11886 SO_EXT:sequence_nullness_or_absence denotes -
T6315 11887-11888 SO_EXT:sequence_nullness_or_absence denotes -
T6316 11889-11897 CL:0002322 denotes ES cells
T6317 11892-11897 CL_GO_EXT:cell denotes cells
T6318 11904-11919 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T6319 11926-11929 CHEBI_SO_EXT:DNA denotes DNA
T6320 11963-11968 SO_EXT:0000704 denotes genes
T6321 11995-12003 CL:0002322 denotes ES cells
T6322 11998-12003 CL_GO_EXT:cell denotes cells
T6323 12005-12009 PR_EXT:000006847 denotes ESG1
T6324 12032-12036 SO_EXT:0000704 denotes gene
T6325 12071-12075 PR_EXT:000006847 denotes ESG1
T6326 12075-12076 SO_EXT:sequence_nullness_or_absence denotes -
T6374 13175-13176 NCBITaxon:10088 denotes e
T6375 13181-13185 PR_EXT:000006847 denotes ESG1
T6376 13185-13186 SO_EXT:sequence_nullness_or_absence denotes -
T6377 13195-13199 NCBITaxon:10088 denotes mice
T6378 13213-13218 GO_EXT:beta_geo denotes β-geo
T6379 13219-13223 PR_EXT:000006847 denotes ESG1
T6380 13223-13224 SO_EXT:normal_or_wild_type_or_present denotes +
T6381 13225-13226 SO_EXT:sequence_nullness_or_absence denotes -
T6382 13227-13234 CL:0002322 denotes ES cell
T6383 13230-13234 CL_GO_EXT:cell denotes cell
T6384 13235-13241 SO_EXT:sequence_cloned_entity denotes clones
T6385 13251-13262 UBERON:0000358 denotes blastocysts
T6386 13273-13277 NCBITaxon:10088 denotes mice
T6387 13324-13330 SO_EXT:sequence_cloned_entity denotes clones
T6388 13344-13348 PR_EXT:000006847 denotes ESG1
T6389 13348-13349 SO_EXT:sequence_nullness_or_absence denotes -
T6390 13350-13351 SO_EXT:sequence_nullness_or_absence denotes -
T6391 13352-13356 NCBITaxon:10088 denotes mice
T6392 13385-13394 SO_EXT:wild_type_entity_or_quality denotes wild-type
T6393 13399-13403 PR_EXT:000006847 denotes ESG1
T6394 13403-13404 SO_EXT:normal_or_wild_type_or_present denotes +
T6395 13405-13406 SO_EXT:sequence_nullness_or_absence denotes -
T6396 13415-13419 PR_EXT:000006847 denotes ESG1
T6397 13419-13420 SO_EXT:sequence_nullness_or_absence denotes -
T6398 13421-13422 SO_EXT:sequence_nullness_or_absence denotes -
T6399 13445-13449 PR_EXT:000006847 denotes ESG1
T6400 13449-13450 SO_EXT:normal_or_wild_type_or_present denotes +
T6402 13453-13457 NCBITaxon:10088 denotes mice
R6 T702 T701 _lexicallyChainedTo 1,Embryonic stem cell-specific gene
R7 T702 T705 _lexicallyChainedTo 1,ESG
R8 T715 T714 _lexicallyChainedTo cells,embryonic stem
R9 T715 T716 _lexicallyChainedTo cells,ES
R10 T797 T796 _lexicallyChainedTo cells,self-renewal of
R319 T2087 T2086 _lexicallyChainedTo cells,Embryonic stem
R320 T2087 T2088 _lexicallyChainedTo cells,ES
R321 T2110 T2109 _lexicallyChainedTo cells,proliferation of
R322 T2139 T2138 _lexicallyChainedTo cells,embryonic
R323 T2153 T2152 _lexicallyChainedTo cells,embryonic
R324 T2157 T2156 _lexicallyChainedTo stem cells,multipotent
R1328 T6245 T6244 _lexicallyChainedTo genes,fusion of
R2890 T10381 T10380 _lexicallyChainedTo antibodies,Generation of
R3167 T11469 T11468 _lexicallyChainedTo cell,primary

craft-sa-dev

Id Subject Object Predicate Lexical cue
T803 0-89 sentence denotes Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5)
T804 15-18 CC denotes and
T805 19-27 VBN denotes targeted
T806 28-38 NN denotes disruption
T807 39-41 IN denotes of
T808 42-45 DT denotes the
T809 52-56 NN denotes gene
T810 46-51 NN denotes mouse
T811 57-65 VBG denotes encoding
T812 66-70 NN denotes ESG1
T813 71-72 -LRB- denotes (
T814 72-76 NN denotes PH34
T815 83-88 NN denotes DPPA5
T816 76-77 HYPH denotes /
T817 77-82 NN denotes ECAT2
T818 82-83 HYPH denotes /
T819 88-89 -RRB- denotes )
T820 89-90 sentence denotes
T821 100-110 sentence denotes Background
T822 100-110 NN denotes Background
T823 110-286 sentence denotes Embryonic stem cell-specific gene (ESG) 1, which encodes a KH-domain containing protein, is specifically expressed in early embryos, germ cells, and embryonic stem (ES) cells.
T824 111-120 JJ denotes Embryonic
T825 126-130 NN denotes cell
T826 121-125 NN denotes stem
T827 131-139 JJ denotes specific
T828 130-131 HYPH denotes -
T829 140-144 NN denotes gene
T830 216-225 VBN denotes expressed
T831 145-146 -LRB- denotes (
T832 146-149 NN denotes ESG
T833 149-150 -RRB- denotes )
T834 151-152 CD denotes 1
T835 152-154 , denotes ,
T836 154-159 WDT denotes which
T837 160-167 VBZ denotes encodes
T838 168-169 DT denotes a
T839 191-198 NN denotes protein
T840 170-172 NN denotes KH
T841 173-179 NN denotes domain
T842 172-173 HYPH denotes -
T843 180-190 VBG denotes containing
T844 198-200 , denotes ,
T845 200-202 VBZ denotes is
T846 203-215 RB denotes specifically
T847 226-228 IN denotes in
T848 229-234 JJ denotes early
T849 235-242 NNS denotes embryos
T850 242-244 , denotes ,
T851 244-248 NN denotes germ
T852 249-254 NNS denotes cells
T853 254-256 , denotes ,
T854 256-259 CC denotes and
T855 260-269 JJ denotes embryonic
T856 270-274 NN denotes stem
T857 280-285 NNS denotes cells
T858 275-276 -LRB- denotes (
T859 276-278 NN denotes ES
T860 278-279 -RRB- denotes )
T861 285-286 . denotes .
T862 286-382 sentence denotes Previous studies identified genomic clones containing the mouse ESG1 gene and five pseudogenes.
T863 287-295 JJ denotes Previous
T864 296-303 NNS denotes studies
T865 304-314 VBD denotes identified
T866 315-322 JJ denotes genomic
T867 323-329 NNS denotes clones
T868 330-340 VBG denotes containing
T869 341-344 DT denotes the
T870 356-360 NN denotes gene
T871 345-350 NN denotes mouse
T872 351-355 NN denotes ESG1
T873 361-364 CC denotes and
T874 365-369 CD denotes five
T875 370-381 NNS denotes pseudogenes
T876 381-382 . denotes .
T877 382-476 sentence denotes However, their chromosomal localizations or physiological functions have not been determined.
T878 383-390 RB denotes However
T879 465-475 VBN denotes determined
T880 390-392 , denotes ,
T881 392-397 PRP$ denotes their
T882 410-423 NNS denotes localizations
T883 398-409 JJ denotes chromosomal
T884 424-426 CC denotes or
T885 427-440 JJ denotes physiological
T886 441-450 NNS denotes functions
T887 451-455 VBP denotes have
T888 456-459 RB denotes not
T889 460-464 VBN denotes been
T890 475-476 . denotes .
T891 476-485 sentence denotes Results
T892 478-485 NNS denotes Results
T893 485-559 sentence denotes A Blast search of mouse genomic databases failed to locate the ESG1 gene.
T894 486-487 DT denotes A
T895 494-500 NN denotes search
T896 488-493 NNP denotes Blast
T897 528-534 VBD denotes failed
T898 501-503 IN denotes of
T899 504-509 NN denotes mouse
T900 518-527 NNS denotes databases
T901 510-517 JJ denotes genomic
T902 535-537 TO denotes to
T903 538-544 VB denotes locate
T904 545-548 DT denotes the
T905 554-558 NN denotes gene
T906 549-553 NN denotes ESG1
T907 558-559 . denotes .
T908 559-726 sentence denotes We identified several bacterial artificial clones containing the mouse ESG1 gene and an additional ESG1-like sequence with a similar gene structure from chromosome 9.
T909 560-562 PRP denotes We
T910 563-573 VBD denotes identified
T911 574-581 JJ denotes several
T912 603-609 NNS denotes clones
T913 582-591 JJ denotes bacterial
T914 592-602 JJ denotes artificial
T915 610-620 VBG denotes containing
T916 621-624 DT denotes the
T917 636-640 NN denotes gene
T918 625-630 NN denotes mouse
T919 631-635 NN denotes ESG1
T920 641-644 CC denotes and
T921 645-647 DT denotes an
T922 669-677 NN denotes sequence
T923 648-658 JJ denotes additional
T924 659-663 NN denotes ESG1
T925 664-668 JJ denotes like
T926 663-664 HYPH denotes -
T927 678-682 IN denotes with
T928 683-684 DT denotes a
T929 698-707 NN denotes structure
T930 685-692 JJ denotes similar
T931 693-697 NN denotes gene
T932 708-712 IN denotes from
T933 713-723 NN denotes chromosome
T934 724-725 CD denotes 9
T935 725-726 . denotes .
T936 726-838 sentence denotes The ESG1-like sequence contained a multiple critical mutations, indicating that it was a duplicated pseudogene.
T937 727-730 DT denotes The
T938 741-749 NN denotes sequence
T939 731-735 NN denotes ESG1
T940 736-740 JJ denotes like
T941 735-736 HYPH denotes -
T942 750-759 VBD denotes contained
T943 760-761 DT denotes a
T944 780-789 NNS denotes mutations
T945 762-770 JJ denotes multiple
T946 771-779 JJ denotes critical
T947 789-791 , denotes ,
T948 791-801 VBG denotes indicating
T949 802-806 IN denotes that
T950 810-813 VBD denotes was
T951 807-809 PRP denotes it
T952 814-815 DT denotes a
T953 827-837 NN denotes pseudogene
T954 816-826 VBN denotes duplicated
T955 837-838 . denotes .
T956 838-1018 sentence denotes The 5' flanking region of the ESG1 gene, but not that of the pseudogene, exhibited strong enhancer and promoter activity in undifferentiated ES cells by luciferase reporter assay.
T957 839-842 DT denotes The
T958 855-861 NN denotes region
T959 843-844 CD denotes 5
T960 844-845 SYM denotes '
T961 846-854 NN denotes flanking
T962 912-921 VBD denotes exhibited
T963 862-864 IN denotes of
T964 865-868 DT denotes the
T965 874-878 NN denotes gene
T966 869-873 NN denotes ESG1
T967 878-880 , denotes ,
T968 880-883 CC denotes but
T969 884-887 RB denotes not
T970 888-892 DT denotes that
T971 893-895 IN denotes of
T972 896-899 DT denotes the
T973 900-910 NN denotes pseudogene
T974 910-912 , denotes ,
T975 922-928 JJ denotes strong
T976 951-959 NN denotes activity
T977 929-937 NN denotes enhancer
T978 938-941 CC denotes and
T979 942-950 NN denotes promoter
T980 960-962 IN denotes in
T981 963-979 JJ denotes undifferentiated
T982 983-988 NNS denotes cells
T983 980-982 NN denotes ES
T984 989-991 IN denotes by
T985 992-1002 NN denotes luciferase
T986 1003-1011 NN denotes reporter
T987 1012-1017 NN denotes assay
T988 1017-1018 . denotes .
T989 1018-1162 sentence denotes To study the physiological functions of the ESG1 gene, we replaced this sequence in ES cells with a β-geo cassette by homologous recombination.
T990 1019-1021 TO denotes To
T991 1022-1027 VB denotes study
T992 1077-1085 VBD denotes replaced
T993 1028-1031 DT denotes the
T994 1046-1055 NNS denotes functions
T995 1032-1045 JJ denotes physiological
T996 1056-1058 IN denotes of
T997 1059-1062 DT denotes the
T998 1068-1072 NN denotes gene
T999 1063-1067 NN denotes ESG1
T1000 1072-1074 , denotes ,
T1001 1074-1076 PRP denotes we
T1002 1086-1090 DT denotes this
T1003 1091-1099 NN denotes sequence
T1004 1100-1102 IN denotes in
T1005 1103-1105 NN denotes ES
T1006 1106-1111 NNS denotes cells
T1007 1112-1116 IN denotes with
T1008 1117-1118 DT denotes a
T1009 1125-1133 NN denotes cassette
T1010 1119-1120 NN denotes β
T1011 1121-1124 NN denotes geo
T1012 1120-1121 HYPH denotes -
T1013 1134-1136 IN denotes by
T1014 1137-1147 JJ denotes homologous
T1015 1148-1161 NN denotes recombination
T1016 1161-1162 . denotes .
T1017 1162-1273 sentence denotes Despite specific expression in early embryos and germ cells, ESG1-/- mice developed normally and were fertile.
T1018 1163-1170 IN denotes Despite
T1019 1237-1246 VBD denotes developed
T1020 1171-1179 JJ denotes specific
T1021 1180-1190 NN denotes expression
T1022 1191-1193 IN denotes in
T1023 1194-1199 JJ denotes early
T1024 1200-1207 NNS denotes embryos
T1025 1208-1211 CC denotes and
T1026 1212-1216 NN denotes germ
T1027 1217-1222 NNS denotes cells
T1028 1222-1224 , denotes ,
T1029 1224-1228 NN denotes ESG1
T1030 1232-1236 NNS denotes mice
T1031 1228-1229 SYM denotes -
T1032 1229-1230 HYPH denotes /
T1033 1230-1231 SYM denotes -
T1034 1247-1255 RB denotes normally
T1035 1256-1259 CC denotes and
T1036 1260-1264 VBD denotes were
T1037 1265-1272 JJ denotes fertile
T1038 1272-1273 . denotes .
T1039 1273-1432 sentence denotes We also generated ESG1-/- ES cells both by a second independent homologous recombination and directly from blastocysts derived from heterozygous intercrosses.
T1040 1274-1276 PRP denotes We
T1041 1282-1291 VBD denotes generated
T1042 1277-1281 RB denotes also
T1043 1292-1296 NN denotes ESG1
T1044 1303-1308 NNS denotes cells
T1045 1296-1297 SYM denotes -
T1046 1297-1298 HYPH denotes /
T1047 1298-1299 SYM denotes -
T1048 1300-1302 NN denotes ES
T1049 1309-1313 CC denotes both
T1050 1314-1316 IN denotes by
T1051 1317-1318 DT denotes a
T1052 1349-1362 NN denotes recombination
T1053 1319-1325 JJ denotes second
T1054 1326-1337 JJ denotes independent
T1055 1338-1348 JJ denotes homologous
T1056 1363-1366 CC denotes and
T1057 1367-1375 RB denotes directly
T1058 1376-1380 IN denotes from
T1059 1381-1392 NNS denotes blastocysts
T1060 1393-1400 VBN denotes derived
T1061 1401-1405 IN denotes from
T1062 1406-1418 JJ denotes heterozygous
T1063 1419-1431 NNS denotes intercrosses
T1064 1431-1432 . denotes .
T1065 1432-1518 sentence denotes Northern blot and western blot analyses confirmed the absence of ESG1 in these cells.
T1066 1433-1441 NNP denotes Northern
T1067 1442-1446 NN denotes blot
T1068 1464-1472 NNS denotes analyses
T1069 1447-1450 CC denotes and
T1070 1451-1458 NNP denotes western
T1071 1459-1463 NN denotes blot
T1072 1473-1482 VBD denotes confirmed
T1073 1483-1486 DT denotes the
T1074 1487-1494 NN denotes absence
T1075 1495-1497 IN denotes of
T1076 1498-1502 NN denotes ESG1
T1077 1503-1505 IN denotes in
T1078 1506-1511 DT denotes these
T1079 1512-1517 NNS denotes cells
T1080 1517-1518 . denotes .
T1081 1518-1601 sentence denotes These ES cells demonstrated normal morphology, proliferation, and differentiation.
T1082 1519-1524 DT denotes These
T1083 1528-1533 NNS denotes cells
T1084 1525-1527 NN denotes ES
T1085 1534-1546 VBD denotes demonstrated
T1086 1547-1553 JJ denotes normal
T1087 1554-1564 NN denotes morphology
T1088 1564-1566 , denotes ,
T1089 1566-1579 NN denotes proliferation
T1090 1579-1581 , denotes ,
T1091 1581-1584 CC denotes and
T1092 1585-1600 NN denotes differentiation
T1093 1600-1601 . denotes .
T1094 1601-1613 sentence denotes Conclusion
T1095 1603-1613 NN denotes Conclusion
T1096 1613-1701 sentence denotes The mouse ESG1 gene, together with a duplicated pseudogene, is located on chromosome 9.
T1097 1614-1617 DT denotes The
T1098 1629-1633 NN denotes gene
T1099 1618-1623 NN denotes mouse
T1100 1624-1628 NN denotes ESG1
T1101 1677-1684 VBN denotes located
T1102 1633-1635 , denotes ,
T1103 1635-1643 RB denotes together
T1104 1644-1648 IN denotes with
T1105 1649-1650 DT denotes a
T1106 1662-1672 NN denotes pseudogene
T1107 1651-1661 VBN denotes duplicated
T1108 1672-1674 , denotes ,
T1109 1674-1676 VBZ denotes is
T1110 1685-1687 IN denotes on
T1111 1688-1698 NN denotes chromosome
T1112 1699-1700 CD denotes 9
T1113 1700-1701 . denotes .
T1114 1701-1851 sentence denotes Despite its specific expression in pluripotent cells and germ cells, ESG1 is dispensable for self-renewal of ES cells and establishment of germcells.
T1115 1702-1709 IN denotes Despite
T1116 1776-1778 VBZ denotes is
T1117 1710-1713 PRP$ denotes its
T1118 1723-1733 NN denotes expression
T1119 1714-1722 JJ denotes specific
T1120 1734-1736 IN denotes in
T1121 1737-1748 JJ denotes pluripotent
T1122 1749-1754 NNS denotes cells
T1123 1755-1758 CC denotes and
T1124 1759-1763 NN denotes germ
T1125 1764-1769 NNS denotes cells
T1126 1769-1771 , denotes ,
T1127 1771-1775 NN denotes ESG1
T1128 1779-1790 JJ denotes dispensable
T1129 1791-1794 IN denotes for
T1130 1795-1799 NN denotes self
T1131 1800-1807 NN denotes renewal
T1132 1799-1800 HYPH denotes -
T1133 1808-1810 IN denotes of
T1134 1811-1813 NN denotes ES
T1135 1814-1819 NNS denotes cells
T1136 1820-1823 CC denotes and
T1137 1824-1837 NN denotes establishment
T1138 1838-1840 IN denotes of
T1139 1841-1850 NNS denotes germcells
T1140 1850-1851 . denotes .
T2208 1864-1873 JJ denotes Embryonic
T2209 1874-1878 NN denotes stem
T2210 1884-1889 NNS denotes cells
T2211 1879-1880 -LRB- denotes (
T2212 1880-1882 NN denotes ES
T2213 1882-1883 -RRB- denotes )
T2214 1901-1908 VBN denotes derived
T2215 1890-1894 VBD denotes were
T2216 1895-1900 RB denotes first
T2217 1909-1913 IN denotes from
T2218 1914-1917 DT denotes the
T2219 1918-1929 NNS denotes blastocysts
T2220 1930-1932 IN denotes of
T2221 1933-1937 NNS denotes mice
T2222 1938-1940 IN denotes in
T2223 1941-1945 CD denotes 1981
T2224 1946-1947 -LRB- denotes [
T2225 1949-1950 CD denotes 2
T2226 1947-1948 CD denotes 1
T2227 1948-1949 , denotes ,
T2228 1950-1951 -RRB- denotes ]
T2229 1952-1955 CC denotes and
T2230 1956-1962 NNS denotes humans
T2231 1963-1965 IN denotes in
T2232 1966-1970 CD denotes 1998
T2233 1971-1972 -LRB- denotes [
T2234 1972-1973 CD denotes 3
T2235 1973-1974 -RRB- denotes ]
T2236 1974-1975 . denotes .
T2237 1975-2145 sentence denotes ES cells have two important properties: theability to maintain pluripotency, which is the ability to differentiate into a wide variety of cells, and rapid proliferation.
T2238 1976-1978 NN denotes ES
T2239 1979-1984 NNS denotes cells
T2240 1985-1989 VBP denotes have
T2241 1990-1993 CD denotes two
T2242 2004-2014 NNS denotes properties
T2243 1994-2003 JJ denotes important
T2244 2014-2016 : denotes :
T2245 2016-2026 NN denotes theability
T2246 2027-2029 TO denotes to
T2247 2030-2038 VB denotes maintain
T2248 2039-2051 NN denotes pluripotency
T2249 2051-2053 , denotes ,
T2250 2053-2058 WDT denotes which
T2251 2059-2061 VBZ denotes is
T2252 2062-2065 DT denotes the
T2253 2066-2073 NN denotes ability
T2254 2074-2076 TO denotes to
T2255 2077-2090 VB denotes differentiate
T2256 2091-2095 IN denotes into
T2257 2096-2097 DT denotes a
T2258 2103-2110 NN denotes variety
T2259 2098-2102 JJ denotes wide
T2260 2111-2113 IN denotes of
T2261 2114-2119 NNS denotes cells
T2262 2119-2121 , denotes ,
T2263 2121-2124 CC denotes and
T2264 2125-2130 JJ denotes rapid
T2265 2131-2144 NN denotes proliferation
T2266 2144-2145 . denotes .
T2267 2145-2240 sentence denotes These characteristics make mouse ES cells an essential component of gene targeting technology.
T2268 2146-2151 DT denotes These
T2269 2152-2167 NNS denotes characteristics
T2270 2168-2172 VBP denotes make
T2271 2173-2178 NN denotes mouse
T2272 2182-2187 NNS denotes cells
T2273 2179-2181 NN denotes ES
T2274 2201-2210 NN denotes component
T2275 2188-2190 DT denotes an
T2276 2191-2200 JJ denotes essential
T2277 2211-2213 IN denotes of
T2278 2214-2218 NN denotes gene
T2279 2219-2228 NN denotes targeting
T2280 2229-2239 NN denotes technology
T2281 2239-2240 . denotes .
T2282 2240-2413 sentence denotes These qualitiesalso make human ES cells attractive sources for cell transplantation therapy to treat various diseases, including spinal cord injuries and juvenile diabetes.
T2283 2241-2246 DT denotes These
T2284 2247-2256 NNS denotes qualities
T2285 2261-2265 VBP denotes make
T2286 2256-2260 RB denotes also
T2287 2266-2271 JJ denotes human
T2288 2275-2280 NNS denotes cells
T2289 2272-2274 NN denotes ES
T2290 2292-2299 NNS denotes sources
T2291 2281-2291 JJ denotes attractive
T2292 2300-2303 IN denotes for
T2293 2304-2308 NN denotes cell
T2294 2309-2324 NN denotes transplantation
T2295 2325-2332 NN denotes therapy
T2296 2333-2335 TO denotes to
T2297 2336-2341 VB denotes treat
T2298 2342-2349 JJ denotes various
T2299 2350-2358 NNS denotes diseases
T2300 2358-2360 , denotes ,
T2301 2360-2369 VBG denotes including
T2302 2370-2376 JJ denotes spinal
T2303 2377-2381 NN denotes cord
T2304 2382-2390 NNS denotes injuries
T2305 2391-2394 CC denotes and
T2306 2395-2403 JJ denotes juvenile
T2307 2404-2412 NN denotes diabetes
T2308 2412-2413 . denotes .
T2309 2413-2571 sentence denotes The molecular mechanisms underlying the pluripotency and rapid proliferation of ES cells are currently a major focus of the field of stem cell biology [4-6].
T2310 2414-2417 DT denotes The
T2311 2428-2438 NNS denotes mechanisms
T2312 2418-2427 JJ denotes molecular
T2313 2503-2506 VBP denotes are
T2314 2439-2449 VBG denotes underlying
T2315 2450-2453 DT denotes the
T2316 2454-2466 NN denotes pluripotency
T2317 2467-2470 CC denotes and
T2318 2471-2476 JJ denotes rapid
T2319 2477-2490 NN denotes proliferation
T2320 2491-2493 IN denotes of
T2321 2494-2496 NN denotes ES
T2322 2497-2502 NNS denotes cells
T2323 2507-2516 RB denotes currently
T2324 2517-2518 DT denotes a
T2325 2525-2530 NN denotes focus
T2326 2519-2524 JJ denotes major
T2327 2531-2533 IN denotes of
T2328 2534-2537 DT denotes the
T2329 2538-2543 NN denotes field
T2330 2544-2546 IN denotes of
T2331 2547-2551 NN denotes stem
T2332 2552-2556 NN denotes cell
T2333 2557-2564 NN denotes biology
T2334 2565-2566 -LRB- denotes [
T2335 2566-2567 CD denotes 4
T2336 2567-2568 SYM denotes -
T2337 2568-2569 CD denotes 6
T2338 2569-2570 -RRB- denotes ]
T2339 2570-2571 . denotes .
T2340 2571-2761 sentence denotes To identify molecules essential in ES cells for these properties, several groups have utilized transcriptome analyses to identify genes specifically expressed in ES cells and early embryos.
T2341 2572-2574 TO denotes To
T2342 2575-2583 VB denotes identify
T2343 2658-2666 VBN denotes utilized
T2344 2584-2593 NNS denotes molecules
T2345 2594-2603 JJ denotes essential
T2346 2604-2606 IN denotes in
T2347 2607-2609 NN denotes ES
T2348 2610-2615 NNS denotes cells
T2349 2616-2619 IN denotes for
T2350 2620-2625 DT denotes these
T2351 2626-2636 NNS denotes properties
T2352 2636-2638 , denotes ,
T2353 2638-2645 JJ denotes several
T2354 2646-2652 NNS denotes groups
T2355 2653-2657 VBP denotes have
T2356 2667-2680 NN denotes transcriptome
T2357 2681-2689 NNS denotes analyses
T2358 2690-2692 TO denotes to
T2359 2693-2701 VB denotes identify
T2360 2702-2707 NNS denotes genes
T2361 2708-2720 RB denotes specifically
T2362 2721-2730 VBN denotes expressed
T2363 2731-2733 IN denotes in
T2364 2734-2736 NN denotes ES
T2365 2737-2742 NNS denotes cells
T2366 2743-2746 CC denotes and
T2367 2747-2752 JJ denotes early
T2368 2753-2760 NNS denotes embryos
T2369 2760-2761 . denotes .
T2370 2761-2954 sentence denotes These analyses, including DNA microarray analyses [7] and expressed sequence tag analyses [8-12], identified several common transcripts, including ESG1 that was also designated dppa5 or ECAT2.
T2371 2762-2767 DT denotes These
T2372 2768-2776 NNS denotes analyses
T2373 2860-2870 VBD denotes identified
T2374 2776-2778 , denotes ,
T2375 2778-2787 VBG denotes including
T2376 2788-2791 NN denotes DNA
T2377 2803-2811 NNS denotes analyses
T2378 2792-2802 NN denotes microarray
T2379 2812-2813 -LRB- denotes [
T2380 2813-2814 CD denotes 7
T2381 2814-2815 -RRB- denotes ]
T2382 2816-2819 CC denotes and
T2383 2820-2829 VBN denotes expressed
T2384 2830-2838 NN denotes sequence
T2385 2843-2851 NNS denotes analyses
T2386 2839-2842 NN denotes tag
T2387 2852-2853 -LRB- denotes [
T2388 2853-2854 CD denotes 8
T2389 2854-2855 SYM denotes -
T2390 2855-2857 CD denotes 12
T2391 2857-2858 -RRB- denotes ]
T2392 2858-2860 , denotes ,
T2393 2871-2878 JJ denotes several
T2394 2886-2897 NNS denotes transcripts
T2395 2879-2885 JJ denotes common
T2396 2897-2899 , denotes ,
T2397 2899-2908 VBG denotes including
T2398 2909-2913 NN denotes ESG1
T2399 2914-2918 WDT denotes that
T2400 2928-2938 VBN denotes designated
T2401 2919-2922 VBD denotes was
T2402 2923-2927 RB denotes also
T2403 2939-2944 NN denotes dppa5
T2404 2945-2947 CC denotes or
T2405 2948-2953 NN denotes ECAT2
T2406 2953-2954 . denotes .
T2407 2954-3082 sentence denotes ESG1 was originally identified as a transcript Ph34 that was down-regulated by retinoic acid in embryonic carcinoma cells [13].
T2408 2955-2959 NN denotes ESG1
T2409 2975-2985 VBN denotes identified
T2410 2960-2963 VBD denotes was
T2411 2964-2974 RB denotes originally
T2412 2986-2988 IN denotes as
T2413 2989-2990 DT denotes a
T2414 3002-3006 NN denotes Ph34
T2415 2991-3001 NN denotes transcript
T2416 3007-3011 WDT denotes that
T2417 3021-3030 VBN denotes regulated
T2418 3012-3015 VBD denotes was
T2419 3016-3020 RB denotes down
T2420 3020-3021 HYPH denotes -
T2421 3031-3033 IN denotes by
T2422 3034-3042 JJ denotes retinoic
T2423 3043-3047 NN denotes acid
T2424 3048-3050 IN denotes in
T2425 3051-3060 JJ denotes embryonic
T2426 3061-3070 NN denotes carcinoma
T2427 3071-3076 NNS denotes cells
T2428 3077-3078 -LRB- denotes [
T2429 3078-3080 CD denotes 13
T2430 3080-3081 -RRB- denotes ]
T2431 3081-3082 . denotes .
T2432 3082-3169 sentence denotes The expression of this gene was confined in mice to early embryos and germ cells [14].
T2433 3083-3086 DT denotes The
T2434 3087-3097 NN denotes expression
T2435 3115-3123 VBN denotes confined
T2436 3098-3100 IN denotes of
T2437 3101-3105 DT denotes this
T2438 3106-3110 NN denotes gene
T2439 3111-3114 VBD denotes was
T2440 3124-3126 IN denotes in
T2441 3127-3131 NNS denotes mice
T2442 3132-3134 IN denotes to
T2443 3135-3140 JJ denotes early
T2444 3141-3148 NNS denotes embryos
T2445 3149-3152 CC denotes and
T2446 3153-3157 NN denotes germ
T2447 3158-3163 NNS denotes cells
T2448 3164-3165 -LRB- denotes [
T2449 3165-3167 CD denotes 14
T2450 3167-3168 -RRB- denotes ]
T2451 3168-3169 . denotes .
T2452 3169-3296 sentence denotes It is also expressed in pluripotent cells, including ES cells, embryonic germ cells, and multipotent germline stem cells [15].
T2453 3170-3172 PRP denotes It
T2454 3181-3190 VBN denotes expressed
T2455 3173-3175 VBZ denotes is
T2456 3176-3180 RB denotes also
T2457 3191-3193 IN denotes in
T2458 3194-3205 JJ denotes pluripotent
T2459 3206-3211 NNS denotes cells
T2460 3211-3213 , denotes ,
T2461 3213-3222 VBG denotes including
T2462 3223-3225 NN denotes ES
T2463 3226-3231 NNS denotes cells
T2464 3231-3233 , denotes ,
T2465 3233-3242 JJ denotes embryonic
T2466 3248-3253 NNS denotes cells
T2467 3243-3247 NN denotes germ
T2468 3253-3255 , denotes ,
T2469 3255-3258 CC denotes and
T2470 3259-3270 JJ denotes multipotent
T2471 3285-3290 NNS denotes cells
T2472 3271-3279 NN denotes germline
T2473 3280-3284 NN denotes stem
T2474 3291-3292 -LRB- denotes [
T2475 3292-3294 CD denotes 15
T2476 3294-3295 -RRB- denotes ]
T2477 3295-3296 . denotes .
T2478 3296-3413 sentence denotes ESG1 encodes a polypeptide of 118 amino acids that contains a single KH domain, which is an RNA-binding domain [16].
T2479 3297-3301 NN denotes ESG1
T2480 3302-3309 VBZ denotes encodes
T2481 3310-3311 DT denotes a
T2482 3312-3323 NN denotes polypeptide
T2483 3324-3326 IN denotes of
T2484 3327-3330 CD denotes 118
T2485 3337-3342 NNS denotes acids
T2486 3331-3336 NN denotes amino
T2487 3343-3347 WDT denotes that
T2488 3348-3356 VBZ denotes contains
T2489 3357-3358 DT denotes a
T2490 3369-3375 NN denotes domain
T2491 3359-3365 JJ denotes single
T2492 3366-3368 NN denotes KH
T2493 3375-3377 , denotes ,
T2494 3377-3382 WDT denotes which
T2495 3383-3385 VBZ denotes is
T2496 3386-3388 DT denotes an
T2497 3401-3407 NN denotes domain
T2498 3389-3392 NN denotes RNA
T2499 3393-3400 VBG denotes binding
T2500 3392-3393 HYPH denotes -
T2501 3408-3409 -LRB- denotes [
T2502 3409-3411 CD denotes 16
T2503 3411-3412 -RRB- denotes ]
T2504 3412-3413 . denotes .
T2505 3413-3530 sentence denotes It remains unclear, however, if ESG1 functions as an RNA-binding protein or the roles it plays in ES cells and mice.
T2506 3414-3416 PRP denotes It
T2507 3417-3424 VBZ denotes remains
T2508 3425-3432 JJ denotes unclear
T2509 3432-3434 , denotes ,
T2510 3434-3441 RB denotes however
T2511 3441-3443 , denotes ,
T2512 3443-3445 IN denotes if
T2513 3451-3460 VBZ denotes functions
T2514 3446-3450 NN denotes ESG1
T2515 3461-3463 IN denotes as
T2516 3464-3466 DT denotes an
T2517 3479-3486 NN denotes protein
T2518 3467-3470 NN denotes RNA
T2519 3471-3478 VBG denotes binding
T2520 3470-3471 HYPH denotes -
T2521 3487-3489 CC denotes or
T2522 3490-3493 DT denotes the
T2523 3494-3499 NNS denotes roles
T2524 3500-3502 PRP denotes it
T2525 3503-3508 VBZ denotes plays
T2526 3509-3511 IN denotes in
T2527 3512-3514 NN denotes ES
T2528 3515-3520 NNS denotes cells
T2529 3521-3524 CC denotes and
T2530 3525-3529 NNS denotes mice
T2531 3529-3530 . denotes .
T2532 3530-3653 sentence denotes Previous genomic library screening by identified genomic clones containing the mouse ESG1 gene and seven pseudogenes [17].
T2533 3531-3539 JJ denotes Previous
T2534 3556-3565 NN denotes screening
T2535 3540-3547 JJ denotes genomic
T2536 3548-3555 NN denotes library
T2537 3566-3568 IN denotes by
T2538 3569-3579 VBN denotes identified
T2539 3588-3594 NNS denotes clones
T2540 3580-3587 JJ denotes genomic
T2541 3595-3605 VBG denotes containing
T2542 3606-3609 DT denotes the
T2543 3621-3625 NN denotes gene
T2544 3610-3615 NN denotes mouse
T2545 3616-3620 NN denotes ESG1
T2546 3626-3629 CC denotes and
T2547 3630-3635 CD denotes seven
T2548 3636-3647 NNS denotes pseudogenes
T2549 3648-3649 -LRB- denotes [
T2550 3649-3651 CD denotes 17
T2551 3651-3652 -RRB- denotes ]
T2552 3652-3653 . denotes .
T2553 3653-3785 sentence denotes Two of these pseudogenes exhibit a similar exon-intron structure as the ESG1 gene, indicating their generation by gene duplication.
T2554 3654-3657 CD denotes Two
T2555 3679-3686 VBP denotes exhibit
T2556 3658-3660 IN denotes of
T2557 3661-3666 DT denotes these
T2558 3667-3678 NNS denotes pseudogenes
T2559 3687-3688 DT denotes a
T2560 3709-3718 NN denotes structure
T2561 3689-3696 JJ denotes similar
T2562 3697-3701 NN denotes exon
T2563 3702-3708 NN denotes intron
T2564 3701-3702 HYPH denotes -
T2565 3719-3721 IN denotes as
T2566 3722-3725 DT denotes the
T2567 3731-3735 NN denotes gene
T2568 3726-3730 NN denotes ESG1
T2569 3735-3737 , denotes ,
T2570 3737-3747 VBG denotes indicating
T2571 3748-3753 PRP$ denotes their
T2572 3754-3764 NN denotes generation
T2573 3765-3767 IN denotes by
T2574 3768-3772 NN denotes gene
T2575 3773-3784 NN denotes duplication
T2576 3784-3785 . denotes .
T2577 3785-3925 sentence denotes The five remaining pseudogenes did not contain any introns, indicating that these were generated by retrotransposition of ESG1 transcripts.
T2578 3786-3789 DT denotes The
T2579 3805-3816 NNS denotes pseudogenes
T2580 3790-3794 CD denotes five
T2581 3795-3804 VBG denotes remaining
T2582 3825-3832 VB denotes contain
T2583 3817-3820 VBD denotes did
T2584 3821-3824 RB denotes not
T2585 3833-3836 DT denotes any
T2586 3837-3844 NNS denotes introns
T2587 3844-3846 , denotes ,
T2588 3846-3856 VBG denotes indicating
T2589 3857-3861 IN denotes that
T2590 3873-3882 VBN denotes generated
T2591 3862-3867 DT denotes these
T2592 3868-3872 VBD denotes were
T2593 3883-3885 IN denotes by
T2594 3886-3904 NN denotes retrotransposition
T2595 3905-3907 IN denotes of
T2596 3908-3912 NN denotes ESG1
T2597 3913-3924 NNS denotes transcripts
T2598 3924-3925 . denotes .
T2599 3925-4028 sentence denotes The chromosomal localizations of the mouse ESG1 gene and pseudogenes, however, have not been reported.
T2600 3926-3929 DT denotes The
T2601 3942-3955 NNS denotes localizations
T2602 3930-3941 JJ denotes chromosomal
T2603 4019-4027 VBN denotes reported
T2604 3956-3958 IN denotes of
T2605 3959-3962 DT denotes the
T2606 3974-3978 NN denotes gene
T2607 3963-3968 NN denotes mouse
T2608 3969-3973 NN denotes ESG1
T2609 3979-3982 CC denotes and
T2610 3983-3994 NNS denotes pseudogenes
T2611 3994-3996 , denotes ,
T2612 3996-4003 RB denotes however
T2613 4003-4005 , denotes ,
T2614 4005-4009 VBP denotes have
T2615 4010-4013 RB denotes not
T2616 4014-4018 VBN denotes been
T2617 4027-4028 . denotes .
T2618 4028-4136 sentence denotes In this study, we determined the structure of the mouse gene encoding this protein and related pseudogenes.
T2619 4029-4031 IN denotes In
T2620 4047-4057 VBD denotes determined
T2621 4032-4036 DT denotes this
T2622 4037-4042 NN denotes study
T2623 4042-4044 , denotes ,
T2624 4044-4046 PRP denotes we
T2625 4058-4061 DT denotes the
T2626 4062-4071 NN denotes structure
T2627 4072-4074 IN denotes of
T2628 4075-4078 DT denotes the
T2629 4085-4089 NN denotes gene
T2630 4079-4084 NN denotes mouse
T2631 4090-4098 VBG denotes encoding
T2632 4099-4103 DT denotes this
T2633 4104-4111 NN denotes protein
T2634 4112-4115 CC denotes and
T2635 4116-4123 JJ denotes related
T2636 4124-4135 NNS denotes pseudogenes
T2637 4135-4136 . denotes .
T2638 4136-4218 sentence denotes We also performed gene targeting to determine the physiological function of ESG1.
T2639 4137-4139 PRP denotes We
T2640 4145-4154 VBD denotes performed
T2641 4140-4144 RB denotes also
T2642 4155-4159 NN denotes gene
T2643 4160-4169 NN denotes targeting
T2644 4170-4172 TO denotes to
T2645 4173-4182 VB denotes determine
T2646 4183-4186 DT denotes the
T2647 4201-4209 NN denotes function
T2648 4187-4200 JJ denotes physiological
T2649 4210-4212 IN denotes of
T2650 4213-4217 NN denotes ESG1
T2651 4217-4218 . denotes .
T4020 4244-4255 JJ denotes Chromosomal
T4021 4256-4268 NN denotes localization
T4022 4269-4272 CC denotes and
T4023 4273-4283 NNS denotes structures
T4024 4284-4286 IN denotes of
T4025 4287-4292 NN denotes mouse
T4026 4298-4302 NN denotes gene
T4027 4293-4297 NN denotes ESG1
T4028 4303-4306 CC denotes and
T4029 4307-4317 NNS denotes psedogenes
T4030 4317-4500 sentence denotes To determine the chromosomal localizations of the mouse ESG1 gene and pseudogenes, we performed a Blast analysis of the mouse genomic database with the ESG1 cDNA sequence as a query.
T4031 4318-4320 TO denotes To
T4032 4321-4330 VB denotes determine
T4033 4404-4413 VBD denotes performed
T4034 4331-4334 DT denotes the
T4035 4347-4360 NNS denotes localizations
T4036 4335-4346 JJ denotes chromosomal
T4037 4361-4363 IN denotes of
T4038 4364-4367 DT denotes the
T4039 4379-4383 NN denotes gene
T4040 4368-4373 NN denotes mouse
T4041 4374-4378 NN denotes ESG1
T4042 4384-4387 CC denotes and
T4043 4388-4399 NNS denotes pseudogenes
T4044 4399-4401 , denotes ,
T4045 4401-4403 PRP denotes we
T4046 4414-4415 DT denotes a
T4047 4422-4430 NN denotes analysis
T4048 4416-4421 NNP denotes Blast
T4049 4431-4433 IN denotes of
T4050 4434-4437 DT denotes the
T4051 4452-4460 NN denotes database
T4052 4438-4443 NN denotes mouse
T4053 4444-4451 JJ denotes genomic
T4054 4461-4465 IN denotes with
T4055 4466-4469 DT denotes the
T4056 4480-4488 NN denotes sequence
T4057 4470-4474 NN denotes ESG1
T4058 4475-4479 NN denotes cDNA
T4059 4489-4491 IN denotes as
T4060 4492-4493 DT denotes a
T4061 4494-4499 NN denotes query
T4062 4499-4500 . denotes .
T4063 4500-4618 sentence denotes We identified several putative pseudogenes without introns on chromosomes 1, 5, 11, 12, 14, 16, 17, and X (Figure 1).
T4064 4501-4503 PRP denotes We
T4065 4504-4514 VBD denotes identified
T4066 4515-4522 JJ denotes several
T4067 4532-4543 NNS denotes pseudogenes
T4068 4523-4531 JJ denotes putative
T4069 4544-4551 IN denotes without
T4070 4552-4559 NNS denotes introns
T4071 4560-4562 IN denotes on
T4072 4563-4574 NNS denotes chromosomes
T4073 4605-4606 NN denotes X
T4074 4575-4576 CD denotes 1
T4075 4576-4578 , denotes ,
T4076 4578-4579 CD denotes 5
T4077 4579-4581 , denotes ,
T4078 4581-4583 CD denotes 11
T4079 4583-4585 , denotes ,
T4080 4585-4587 CD denotes 12
T4081 4587-4589 , denotes ,
T4082 4589-4591 CD denotes 14
T4083 4591-4593 , denotes ,
T4084 4593-4595 CD denotes 16
T4085 4595-4597 , denotes ,
T4086 4597-4599 CD denotes 17
T4087 4599-4601 , denotes ,
T4088 4601-4604 CC denotes and
T4089 4607-4608 -LRB- denotes (
T4090 4608-4614 NN denotes Figure
T4091 4615-4616 CD denotes 1
T4092 4616-4617 -RRB- denotes )
T4093 4617-4618 . denotes .
T4094 4618-4750 sentence denotes In addition, two intron-less pseudogenes were identified in DNA fragments for which the chromosomal localization remained unmapped.
T4122 4802-4804 IN denotes to
T4123 4805-4809 NN denotes ESG1
T4124 4810-4814 NN denotes cDNA
T4125 4814-4816 , denotes ,
T4126 4816-4820 PRP denotes they
T4127 4821-4826 MD denotes could
T4128 4827-4830 RB denotes not
T4129 4839-4849 JJ denotes functional
T4130 4850-4858 NN denotes proteins
T4131 4858-4860 , denotes ,
T4132 4860-4867 IN denotes because
T4133 4868-4870 IN denotes of
T4134 4871-4879 JJ denotes critical
T4135 4880-4889 NNS denotes mutations
T4136 4889-4890 . denotes .
T4137 4890-4998 sentence denotes This result suggests that there are a larger number of intron-less pseudogenes than previously anticipated.
T4138 4891-4895 DT denotes This
T4139 4896-4902 NN denotes result
T4140 4903-4911 VBZ denotes suggests
T4141 4912-4916 IN denotes that
T4142 4923-4926 VBP denotes are
T4143 4917-4922 EX denotes there
T4144 4927-4928 DT denotes a
T4145 4936-4942 NN denotes number
T4146 4929-4935 JJR denotes larger
T4147 4943-4945 IN denotes of
T4148 4946-4957 JJ denotes intron-less
T4149 4958-4969 NNS denotes pseudogenes
T4150 4970-4974 IN denotes than
T4151 4986-4997 VBN denotes anticipated
T4152 4975-4985 RB denotes previously
T4153 4997-4998 . denotes .
T4154 4998-5092 sentence denotes Existence of multiple retropseudogenes is a hallmark of pluripotent cell-specific genes [18].
T4155 4999-5008 NN denotes Existence
T4156 5038-5040 VBZ denotes is
T4157 5009-5011 IN denotes of
T4158 5012-5020 JJ denotes multiple
T4159 5021-5037 NNS denotes retropseudogenes
T4160 5041-5042 DT denotes a
T4161 5043-5051 NN denotes hallmark
T4162 5052-5054 IN denotes of
T4163 5055-5066 JJ denotes pluripotent
T4164 5081-5086 NNS denotes genes
T4165 5067-5071 NN denotes cell
T4166 5072-5080 JJ denotes specific
T4167 5071-5072 HYPH denotes -
T4168 5087-5088 -LRB- denotes [
T4169 5088-5090 CD denotes 18
T4170 5090-5091 -RRB- denotes ]
T4171 5091-5092 . denotes .
T4172 5092-5093 sentence denotes
T4187 5396-5400 NN denotes ESG1
T4188 5406-5410 WDT denotes that
T4189 5411-5419 VBD denotes included
T4190 5420-5423 CD denotes two
T4191 5433-5440 NNS denotes introns
T4192 5424-5432 JJ denotes putative
T4193 5440-5441 . denotes .
T4194 5441-5549 sentence denotes These putative first and second exons, however, contained (4) multiple mutations of the ESG1 cDNA sequence.
T4195 5442-5447 DT denotes These
T4196 5474-5479 NNS denotes exons
T4197 5448-5456 JJ denotes putative
T4198 5457-5462 JJ denotes first
T4199 5463-5466 CC denotes and
T4200 5467-5473 JJ denotes second
T4201 5490-5499 VBD denotes contained
T4202 5479-5481 , denotes ,
T4203 5481-5488 RB denotes however
T4204 5488-5490 , denotes ,
T4205 5500-5501 -LRB- denotes (
T4206 5501-5502 CD denotes 4
T4207 5502-5503 -RRB- denotes )
T4208 5504-5512 JJ denotes multiple
T4209 5513-5522 NNS denotes mutations
T4210 5523-5525 IN denotes of
T4211 5526-5529 DT denotes the
T4212 5540-5548 NN denotes sequence
T4213 5530-5534 NN denotes ESG1
T4214 5535-5539 NN denotes cDNA
T4215 5548-5549 . denotes .
T4216 5549-5633 sentence denotes The putative third exon was identical to that of the previously reported ESG1 gene.
T4217 5550-5553 DT denotes The
T4218 5569-5573 NN denotes exon
T4219 5554-5562 JJ denotes putative
T4220 5563-5568 JJ denotes third
T4221 5574-5577 VBD denotes was
T4222 5578-5587 JJ denotes identical
T4223 5588-5590 IN denotes to
T4224 5591-5595 DT denotes that
T4225 5596-5598 IN denotes of
T4226 5599-5602 DT denotes the
T4227 5628-5632 NN denotes gene
T4228 5603-5613 RB denotes previously
T4229 5614-5622 VBN denotes reported
T4230 5623-5627 NN denotes ESG1
T4231 5632-5633 . denotes .
T4232 5633-5763 sentence denotes Also on chromosome 9, we identified another DNA fragment that was similar, but not identical, to the third exon of the ESG1 gene.
T4233 5634-5638 RB denotes Also
T4234 5659-5669 VBD denotes identified
T4235 5639-5641 IN denotes on
T4236 5642-5652 NN denotes chromosome
T4237 5653-5654 CD denotes 9
T4238 5654-5656 , denotes ,
T4239 5656-5658 PRP denotes we
T4240 5670-5677 DT denotes another
T4241 5682-5690 NN denotes fragment
T4242 5678-5681 NN denotes DNA
T4243 5691-5695 WDT denotes that
T4244 5696-5699 VBD denotes was
T4245 5700-5707 JJ denotes similar
T4246 5707-5709 , denotes ,
T4247 5709-5712 CC denotes but
T4248 5713-5716 RB denotes not
T4249 5717-5726 JJ denotes identical
T4250 5726-5728 , denotes ,
T4251 5728-5730 IN denotes to
T4252 5731-5734 DT denotes the
T4253 5741-5745 NN denotes exon
T4254 5735-5740 JJ denotes third
T4255 5746-5748 IN denotes of
T4256 5749-5752 DT denotes the
T4257 5758-5762 NN denotes gene
T4258 5753-5757 NN denotes ESG1
T4259 5762-5763 . denotes .
T4260 5763-5868 sentence denotes These findings suggest that these ESG1-like sequences on chromosome 9 have not been correctly assembled.
T4261 5764-5769 DT denotes These
T4262 5770-5778 NNS denotes findings
T4263 5779-5786 VBP denotes suggest
T4264 5787-5791 IN denotes that
T4265 5858-5867 VBN denotes assembled
T4266 5792-5797 DT denotes these
T4267 5808-5817 NNS denotes sequences
T4268 5798-5802 NN denotes ESG1
T4269 5803-5807 JJ denotes like
T4270 5802-5803 HYPH denotes -
T4271 5818-5820 IN denotes on
T4272 5821-5831 NN denotes chromosome
T4273 5832-5833 CD denotes 9
T4274 5834-5838 VBP denotes have
T4275 5839-5842 RB denotes not
T4276 5843-5847 VBN denotes been
T4277 5848-5857 RB denotes correctly
T4278 5867-5868 . denotes .
T4279 5868-6067 sentence denotes To obtain DNA fragments containing the ESG1 gene, we screened the bacterial artificial chromosome (BAC) DNA pool by PCR using primer pairs that would only amplify the real gene, not the pseudogenes.
T4280 5869-5871 TO denotes To
T4281 5872-5878 VB denotes obtain
T4282 5922-5930 VBD denotes screened
T4283 5879-5882 NN denotes DNA
T4284 5883-5892 NNS denotes fragments
T4285 5893-5903 VBG denotes containing
T4286 5904-5907 DT denotes the
T4287 5913-5917 NN denotes gene
T4288 5908-5912 NN denotes ESG1
T4289 5917-5919 , denotes ,
T4290 5919-5921 PRP denotes we
T4291 5931-5934 DT denotes the
T4292 5977-5981 NN denotes pool
T4293 5935-5944 JJ denotes bacterial
T4294 5956-5966 NN denotes chromosome
T4295 5945-5955 JJ denotes artificial
T4296 5967-5968 -LRB- denotes (
T4297 5968-5971 NN denotes BAC
T4298 5971-5972 -RRB- denotes )
T4299 5973-5976 NN denotes DNA
T4300 5982-5984 IN denotes by
T4301 5985-5988 NN denotes PCR
T4302 5989-5994 VBG denotes using
T4303 5995-6001 NN denotes primer
T4304 6002-6007 NNS denotes pairs
T4305 6008-6012 WDT denotes that
T4306 6024-6031 VB denotes amplify
T4307 6013-6018 MD denotes would
T4308 6019-6023 RB denotes only
T4309 6032-6035 DT denotes the
T4310 6041-6045 NN denotes gene
T4311 6036-6040 JJ denotes real
T4312 6045-6047 , denotes ,
T4313 6047-6050 RB denotes not
T4314 6055-6066 NNS denotes pseudogenes
T4315 6051-6054 DT denotes the
T4316 6066-6067 . denotes .
T4317 6067-6124 sentence denotes We obtained two independent, but overlapping BAC clones.
T4318 6068-6070 PRP denotes We
T4319 6071-6079 VBD denotes obtained
T4320 6080-6083 CD denotes two
T4321 6117-6123 NNS denotes clones
T4322 6084-6095 JJ denotes independent
T4323 6095-6097 , denotes ,
T4324 6097-6100 CC denotes but
T4325 6101-6112 VBG denotes overlapping
T4326 6113-6116 NN denotes BAC
T4327 6123-6124 . denotes .
T4328 6124-6316 sentence denotes Southern blot analyses and sequencing demonstrated that these clones contained a sequence exhibiting complete identity with ESG1 cDNA that was interrupted by two putative introns (Figure 2A).
T4329 6125-6133 NNP denotes Southern
T4330 6134-6138 NN denotes blot
T4331 6163-6175 VBD denotes demonstrated
T4332 6139-6147 NNS denotes analyses
T4333 6148-6151 CC denotes and
T4334 6152-6162 NN denotes sequencing
T4335 6176-6180 IN denotes that
T4336 6194-6203 VBD denotes contained
T4337 6181-6186 DT denotes these
T4338 6187-6193 NNS denotes clones
T4339 6204-6205 DT denotes a
T4340 6206-6214 NN denotes sequence
T4341 6215-6225 VBG denotes exhibiting
T4342 6226-6234 JJ denotes complete
T4343 6235-6243 NN denotes identity
T4344 6244-6248 IN denotes with
T4345 6249-6253 NN denotes ESG1
T4346 6254-6258 NN denotes cDNA
T4347 6259-6263 WDT denotes that
T4348 6268-6279 VBN denotes interrupted
T4349 6264-6267 VBD denotes was
T4350 6280-6282 IN denotes by
T4351 6283-6286 CD denotes two
T4352 6296-6303 NNS denotes introns
T4353 6287-6295 JJ denotes putative
T4354 6304-6305 -LRB- denotes (
T4355 6312-6314 NN denotes 2A
T4356 6305-6311 NN denotes Figure
T4357 6314-6315 -RRB- denotes )
T4358 6315-6316 . denotes .
T4359 6316-6435 sentence denotes The two intron sequences begin with GT and terminate with AG, fulfilling the GT-AG rule of exon-intron junctions [19].
T4360 6317-6320 DT denotes The
T4361 6332-6341 NNS denotes sequences
T4362 6321-6324 CD denotes two
T4363 6325-6331 NN denotes intron
T4364 6342-6347 VBP denotes begin
T4365 6348-6352 IN denotes with
T4366 6353-6355 NN denotes GT
T4367 6356-6359 CC denotes and
T4368 6360-6369 VB denotes terminate
T4369 6370-6374 IN denotes with
T4395 6462-6466 DT denotes this
T4396 6471-6479 NN denotes fragment
T4397 6467-6470 NN denotes DNA
T4398 6490-6496 JJ denotes strong
T4399 6515-6523 NN denotes activity
T4400 6497-6505 NN denotes promoter
T4401 6506-6514 NN denotes enhancer
T4402 6505-6506 HYPH denotes /
T4403 6524-6526 IN denotes by
T4404 6527-6537 NN denotes luciferase
T4405 6547-6553 NNS denotes assays
T4406 6538-6546 NN denotes reporter
T4407 6554-6556 IN denotes in
T4408 6557-6573 JJ denotes undifferentiated
T4409 6577-6582 NNS denotes cells
T4410 6574-6576 NN denotes ES
T4411 6582-6584 , denotes ,
T4412 6584-6587 CC denotes but
T4413 6588-6591 RB denotes not
T4414 6592-6594 IN denotes in
T4415 6595-6602 JJ denotes somatic
T4416 6603-6608 NNS denotes cells
T4417 6609-6610 -LRB- denotes (
T4418 6610-6616 NN denotes Figure
T4419 6617-6618 CD denotes 3
T4420 6618-6619 -RRB- denotes )
T4421 6619-6620 . denotes .
T4422 6620-6719 sentence denotes The same fragment showed much weaker activity after induction of differentiation by retinoic acid.
T4423 6621-6624 DT denotes The
T4424 6630-6638 NN denotes fragment
T4425 6625-6629 JJ denotes same
T4426 6639-6645 VBD denotes showed
T4427 6646-6650 RB denotes much
T4428 6651-6657 JJR denotes weaker
T4429 6658-6666 NN denotes activity
T4430 6667-6672 IN denotes after
T4431 6673-6682 NN denotes induction
T4432 6683-6685 IN denotes of
T4433 6686-6701 NN denotes differentiation
T4434 6702-6704 IN denotes by
T4435 6705-6713 JJ denotes retinoic
T4436 6714-6718 NN denotes acid
T4437 6718-6719 . denotes .
T4438 6719-6769 sentence denotes We concluded that this sequence is the ESG1 gene.
T4439 6720-6722 PRP denotes We
T4440 6723-6732 VBD denotes concluded
T4441 6733-6737 IN denotes that
T4442 6752-6754 VBZ denotes is
T4443 6738-6742 DT denotes this
T4444 6743-6751 NN denotes sequence
T4445 6755-6758 DT denotes the
T4446 6764-6768 NN denotes gene
T4447 6759-6763 NN denotes ESG1
T4448 6768-6769 . denotes .
T4487 7807-7808 . denotes .
T4488 7808-7910 sentence denotes The ESG1-like sequence exhibited greater than 95% identity to the exons and introns of the ESG1 gene.
T4489 7809-7812 DT denotes The
T4490 7823-7831 NN denotes sequence
T4491 7813-7817 NN denotes ESG1
T4492 7818-7822 JJ denotes like
T4493 7817-7818 HYPH denotes -
T4494 7832-7841 VBD denotes exhibited
T4495 7842-7849 JJR denotes greater
T4496 7855-7857 CD denotes 95
T4497 7850-7854 IN denotes than
T4498 7857-7858 NN denotes %
T4499 7859-7867 NN denotes identity
T4500 7868-7870 IN denotes to
T4501 7871-7874 DT denotes the
T4502 7875-7880 NNS denotes exons
T4503 7881-7884 CC denotes and
T4504 7885-7892 NNS denotes introns
T4505 7893-7895 IN denotes of
T4506 7896-7899 DT denotes the
T4507 7905-7909 NN denotes gene
T4508 7900-7904 NN denotes ESG1
T4509 7909-7910 . denotes .
T4510 7910-8050 sentence denotes This sequence, however, contained critical nucleotide substitutions in all of the exons and one nucleotide insertion in exon 2 (Figure 2B).
T4511 7911-7915 DT denotes This
T4512 7916-7924 NN denotes sequence
T4513 7935-7944 VBD denotes contained
T4514 7924-7926 , denotes ,
T4515 7926-7933 RB denotes however
T4516 7933-7935 , denotes ,
T4517 7945-7953 JJ denotes critical
T4518 7965-7978 NNS denotes substitutions
T4519 7954-7964 NN denotes nucleotide
T4520 7979-7981 IN denotes in
T4521 7982-7985 DT denotes all
T4522 7986-7988 IN denotes of
T4523 7989-7992 DT denotes the
T4524 7993-7998 NNS denotes exons
T4525 7999-8002 CC denotes and
T4526 8003-8006 CD denotes one
T4527 8018-8027 NN denotes insertion
T4528 8007-8017 NN denotes nucleotide
T4529 8028-8030 IN denotes in
T4530 8031-8035 NN denotes exon
T4531 8036-8037 CD denotes 2
T4532 8038-8039 -LRB- denotes (
T4533 8046-8048 NN denotes 2B
T4534 8039-8045 NN denotes Figure
T4535 8048-8049 -RRB- denotes )
T4536 8049-8050 . denotes .
T4537 8050-8221 sentence denotes Although 675 base pairs of the 3' flanking regions were conserved between the ESG1 gene and the pseudogene, only five base pairs of the 5' flanking region were identical.
T4538 8051-8059 IN denotes Although
T4539 8107-8116 VBN denotes conserved
T4540 8060-8063 CD denotes 675
T4541 8069-8074 NNS denotes pairs
T4542 8064-8068 NN denotes base
T4543 8075-8077 IN denotes of
T4544 8078-8081 DT denotes the
T4545 8094-8101 NNS denotes regions
T4546 8082-8083 CD denotes 3
T4547 8083-8084 SYM denotes '
T4548 8085-8093 NN denotes flanking
T4549 8102-8106 VBD denotes were
T4550 8206-8210 VBD denotes were
T4551 8117-8124 IN denotes between
T4552 8125-8128 DT denotes the
T4553 8134-8138 NN denotes gene
T4554 8129-8133 NN denotes ESG1
T4555 8139-8142 CC denotes and
T4556 8143-8146 DT denotes the
T4557 8147-8157 NN denotes pseudogene
T4558 8157-8159 , denotes ,
T4559 8159-8163 RB denotes only
T4560 8174-8179 NNS denotes pairs
T4561 8164-8168 CD denotes five
T4562 8169-8173 NN denotes base
T4563 8180-8182 IN denotes of
T4564 8183-8186 DT denotes the
T4565 8199-8205 NN denotes region
T4566 8187-8188 CD denotes 5
T4567 8188-8189 SYM denotes '
T4568 8190-8198 NN denotes flanking
T4569 8211-8220 JJ denotes identical
T4570 8220-8221 . denotes .
T4571 8221-8343 sentence denotes This 5' flanking region (~6 kbp) did not possess any promoter/enhancer activity in luciferase reporter assays (Figure 3).
T4572 8222-8226 DT denotes This
T4573 8239-8245 NN denotes region
T4574 8227-8228 CD denotes 5
T4575 8228-8229 SYM denotes '
T4576 8230-8238 NN denotes flanking
T4577 8263-8270 VB denotes possess
T4578 8246-8247 -LRB- denotes (
T4579 8250-8253 NN denotes kbp
T4580 8247-8248 SYM denotes ~
T4581 8248-8249 CD denotes 6
T4582 8253-8254 -RRB- denotes )
T4583 8255-8258 VBD denotes did
T4584 8259-8262 RB denotes not
T4585 8271-8274 DT denotes any
T4586 8293-8301 NN denotes activity
T4587 8275-8283 NN denotes promoter
T4588 8284-8292 NN denotes enhancer
T4589 8283-8284 HYPH denotes /
T4590 8302-8304 IN denotes in
T4591 8305-8315 NN denotes luciferase
T4592 8316-8324 NN denotes reporter
T4593 8325-8331 NNS denotes assays
T4594 8332-8333 -LRB- denotes (
T4595 8333-8339 NN denotes Figure
T4596 8340-8341 CD denotes 3
T4597 8341-8342 -RRB- denotes )
T4598 8342-8343 . denotes .
T4599 8343-8438 sentence denotes It is thus unlikely that this sequence is transcribed or translated into a functional protein.
T4600 8344-8346 PRP denotes It
T4601 8347-8349 VBZ denotes is
T4602 8350-8354 RB denotes thus
T4603 8355-8363 JJ denotes unlikely
T4604 8364-8368 IN denotes that
T4605 8386-8397 VBN denotes transcribed
T4606 8369-8373 DT denotes this
T4607 8374-8382 NN denotes sequence
T4608 8383-8385 VBZ denotes is
T4609 8398-8400 CC denotes or
T4610 8401-8411 VBN denotes translated
T4611 8412-8416 IN denotes into
T4612 8417-8418 DT denotes a
T4613 8430-8437 NN denotes protein
T4614 8419-8429 JJ denotes functional
T4615 8437-8438 . denotes .
T4616 8438-8496 sentence denotes This sequence likely represents a duplication pseudogene.
T4617 8439-8443 DT denotes This
T4618 8444-8452 NN denotes sequence
T4619 8460-8470 VBZ denotes represents
T4620 8453-8459 RB denotes likely
T4621 8471-8472 DT denotes a
T4622 8485-8495 NN denotes pseudogene
T4623 8473-8484 NN denotes duplication
T4624 8495-8496 . denotes .
T4625 8496-8619 sentence denotes Bierbaum previously reported the existence of two pseudogenes with similar exon-intron organization as the ESG1 gene [17].
T4626 8497-8505 NN denotes Bierbaum
T4627 8517-8525 VBD denotes reported
T4628 8506-8516 RB denotes previously
T4629 8526-8529 DT denotes the
T4630 8530-8539 NN denotes existence
T4631 8540-8542 IN denotes of
T4632 8543-8546 CD denotes two
T4633 8547-8558 NNS denotes pseudogenes
T4634 8559-8563 IN denotes with
T4635 8564-8571 JJ denotes similar
T4636 8584-8596 NN denotes organization
T4637 8572-8576 NN denotes exon
T4638 8577-8583 NN denotes intron
T4639 8576-8577 HYPH denotes -
T4640 8597-8599 IN denotes as
T4641 8600-8603 DT denotes the
T4642 8609-8613 NN denotes gene
T4643 8604-8608 NN denotes ESG1
T4644 8614-8615 -LRB- denotes [
T4645 8615-8617 CD denotes 17
T4646 8617-8618 -RRB- denotes ]
T4647 8618-8619 . denotes .
T4648 8619-8755 sentence denotes We could not determine which of these two pseudogenes corresponds to the one we identified or the location of the remaining pseudogene.
T4649 8620-8622 PRP denotes We
T4650 8633-8642 VB denotes determine
T4651 8623-8628 MD denotes could
T4652 8629-8632 RB denotes not
T4653 8643-8648 WDT denotes which
T4654 8674-8685 VBZ denotes corresponds
T4655 8649-8651 IN denotes of
T4656 8652-8657 DT denotes these
T4657 8662-8673 NNS denotes pseudogenes
T4658 8658-8661 CD denotes two
T4659 8686-8688 IN denotes to
T4660 8689-8692 DT denotes the
T4661 8693-8696 CD denotes one
T4662 8697-8699 PRP denotes we
T4663 8700-8710 VBD denotes identified
T4664 8711-8713 CC denotes or
T4665 8714-8717 DT denotes the
T4666 8718-8726 NN denotes location
T4667 8727-8729 IN denotes of
T4668 8730-8733 DT denotes the
T4669 8744-8754 NN denotes pseudogene
T4670 8734-8743 VBG denotes remaining
T4671 8754-8755 . denotes .
T6437 8757-8765 VBN denotes Targeted
T6438 8766-8776 NN denotes disruption
T6439 8777-8779 IN denotes of
T6440 8780-8783 DT denotes the
T6441 8795-8799 NN denotes gene
T6442 8784-8789 NN denotes mouse
T6443 8790-8794 NN denotes ESG1
T6444 8799-8897 sentence denotes To study the function of ESG1, we deleted the gene by homologous recombination in mouse ES cells.
T6445 8800-8802 TO denotes To
T6446 8803-8808 VB denotes study
T6447 8834-8841 VBD denotes deleted
T6448 8809-8812 DT denotes the
T6449 8813-8821 NN denotes function
T6450 8822-8824 IN denotes of
T6451 8825-8829 NN denotes ESG1
T6452 8829-8831 , denotes ,
T6453 8831-8833 PRP denotes we
T6454 8842-8845 DT denotes the
T6455 8846-8850 NN denotes gene
T6456 8851-8853 IN denotes by
T6457 8854-8864 JJ denotes homologous
T6458 8865-8878 NN denotes recombination
T6459 8879-8881 IN denotes in
T6460 8882-8887 NN denotes mouse
T6461 8891-8896 NNS denotes cells
T6462 8888-8890 NN denotes ES
T6463 8896-8897 . denotes .
T6464 8897-9136 sentence denotes We replaced the three exons with either a fusion of the neomycin-resistance and β-galactosidase genes (β-geo) or the hygromycin resistant gene (HygR) using two targeting vectors (Figure 4A) introduced into RF8 ES cells by electroporation.
T6465 8898-8900 PRP denotes We
T6466 8901-8909 VBD denotes replaced
T6467 8910-8913 DT denotes the
T6468 8920-8925 NNS denotes exons
T6469 8914-8919 CD denotes three
T6470 8926-8930 IN denotes with
T6471 8931-8937 CC denotes either
T6472 8940-8946 NN denotes fusion
T6473 8938-8939 DT denotes a
T6474 8947-8949 IN denotes of
T6475 8950-8953 DT denotes the
T6476 8994-8999 NNS denotes genes
T6477 8954-8962 NN denotes neomycin
T6478 8963-8973 NN denotes resistance
T6479 8962-8963 HYPH denotes -
T6480 8974-8977 CC denotes and
T6481 8978-8979 NN denotes β
T6482 8980-8993 NN denotes galactosidase
T6483 8979-8980 HYPH denotes -
T6484 9000-9001 -LRB- denotes (
T6485 9003-9006 NN denotes geo
T6486 9001-9002 NN denotes β
T6487 9002-9003 HYPH denotes -
T6488 9006-9007 -RRB- denotes )
T6489 9008-9010 CC denotes or
T6490 9011-9014 DT denotes the
T6491 9036-9040 NN denotes gene
T6517 9163-9169 NNS denotes clones
T6518 9155-9157 NN denotes ES
T6519 9158-9162 NN denotes cell
T6520 9170-9174 IN denotes with
T6521 9175-9182 JJ denotes correct
T6522 9194-9207 NN denotes recombination
T6523 9183-9193 JJ denotes homologous
T6524 9208-9210 IN denotes of
T6525 9211-9214 DT denotes the
T6526 9231-9237 NN denotes vector
T6527 9215-9216 NN denotes β
T6528 9217-9220 NN denotes geo
T6529 9216-9217 HYPH denotes -
T6530 9221-9230 NN denotes targeting
T6531 9237-9239 , denotes ,
T6532 9239-9244 WDT denotes which
T6533 9249-9258 VBN denotes confirmed
T6534 9245-9248 VBD denotes was
T6535 9259-9261 IN denotes by
T6536 9262-9270 NNP denotes Southern
T6537 9271-9275 NN denotes blot
T6538 9276-9284 NN denotes analysis
T6539 9285-9286 -LRB- denotes (
T6540 9293-9295 NN denotes 4B
T6541 9286-9292 NN denotes Figure
T6542 9295-9296 -RRB- denotes )
T6543 9296-9297 . denotes .
T6544 9297-9392 sentence denotes We obtained only one clone with correct homologous recombination of the HygR targeting vector.
T6545 9298-9300 PRP denotes We
T6546 9301-9309 VBD denotes obtained
T6547 9310-9314 RB denotes only
T6548 9319-9324 NN denotes clone
T6549 9315-9318 CD denotes one
T6550 9325-9329 IN denotes with
T6551 9330-9337 JJ denotes correct
T6552 9349-9362 NN denotes recombination
T6553 9338-9348 JJ denotes homologous
T6554 9363-9365 IN denotes of
T6555 9366-9369 DT denotes the
T6556 9385-9391 NN denotes vector
T6557 9370-9374 NN denotes HygR
T6558 9375-9384 NN denotes targeting
T6559 9391-9392 . denotes .
T6560 9392-9393 sentence denotes
T6561 10268-10272 sentence denotes To o
T6562 10268-10269 VBN denotes T
T6563 10269-10270 NN denotes o
T6564 10271-10272 IN denotes o
T6565 10272-10274 sentence denotes bt
T6566 10272-10273 IN denotes b
T6567 10273-10274 JJ denotes t
T6568 10274-10280 sentence denotes ain ho
T6569 10278-10280 VBN denotes ho
T6570 10274-10275 CC denotes a
T6611 10407-10409 , denotes ,
T6612 10409-10411 CD denotes 49
T6613 10417-10427 JJ denotes homozygous
T6614 10428-10431 IN denotes for
T6615 10432-10436 NN denotes ESG1
T6616 10437-10445 NN denotes deletion
T6617 10445-10446 . denotes .
T6618 10446-10544 sentence denotes Northern blot and western blot analyses confirmed the absence of ESG1 in these cells (Figure 4C).
T6619 10447-10455 NNP denotes Northern
T6620 10456-10460 NN denotes blot
T6621 10478-10486 NNS denotes analyses
T6622 10461-10464 CC denotes and
T6623 10465-10472 NNP denotes western
T6624 10473-10477 NN denotes blot
T6625 10487-10496 VBD denotes confirmed
T6626 10497-10500 DT denotes the
T6627 10501-10508 NN denotes absence
T6628 10509-10511 IN denotes of
T6629 10512-10516 NN denotes ESG1
T6630 10517-10519 IN denotes in
T6631 10520-10525 DT denotes these
T6632 10526-10531 NNS denotes cells
T6633 10532-10533 -LRB- denotes (
T6634 10540-10542 NN denotes 4C
T6635 10533-10539 NN denotes Figure
T6636 10542-10543 -RRB- denotes )
T6637 10543-10544 . denotes .
T6638 10544-10648 sentence denotes In 29 clones, the β-geo vector had replaced the HygR vector, such that the cells remained heterozygous.
T6639 10545-10547 IN denotes In
T6640 10580-10588 VBN denotes replaced
T6641 10548-10550 CD denotes 29
T6642 10551-10557 NNS denotes clones
T6643 10557-10559 , denotes ,
T6644 10559-10562 DT denotes the
T6645 10569-10575 NN denotes vector
T6646 10563-10564 NN denotes β
T6647 10565-10568 NN denotes geo
T6648 10564-10565 HYPH denotes -
T6649 10576-10579 VBD denotes had
T6650 10589-10592 DT denotes the
T6651 10598-10604 NN denotes vector
T6652 10593-10597 NN denotes HygR
T6653 10604-10606 , denotes ,
T6654 10606-10610 JJ denotes such
T6655 10626-10634 VBD denotes remained
T6656 10611-10615 IN denotes that
T6657 10616-10619 DT denotes the
T6658 10620-10625 NNS denotes cells
T6659 10635-10647 JJ denotes heterozygous
T6660 10647-10648 . denotes .
T6661 10648-10733 sentence denotes In the remaining 27 clones, the β-geo vector was integrated at non-homologous sites.
T6662 10649-10651 IN denotes In
T6663 10698-10708 VBN denotes integrated
T6664 10652-10655 DT denotes the
T6665 10669-10675 NNS denotes clones
T6666 10656-10665 VBG denotes remaining
T6667 10666-10668 CD denotes 27
T6668 10675-10677 , denotes ,
T6669 10677-10680 DT denotes the
T6670 10687-10693 NN denotes vector
T6671 10681-10682 NN denotes β
T6672 10683-10686 NN denotes geo
T6673 10682-10683 HYPH denotes -
T6674 10694-10697 VBD denotes was
T6675 10709-10711 IN denotes at
T6676 10712-10726 JJ denotes non-homologous
T6677 10727-10732 NNS denotes sites
T6678 10732-10733 . denotes .
T6679 10733-10791 sentence denotes ESG1-/- ES cells exhibited normal morphology (Figure 5A).
T6680 10734-10738 NN denotes ESG1
T6681 10745-10750 NNS denotes cells
T6682 10738-10739 SYM denotes -
T6683 10739-10740 HYPH denotes /
T6684 10740-10741 SYM denotes -
T6685 10742-10744 NN denotes ES
T6686 10751-10760 VBD denotes exhibited
T6687 10761-10767 JJ denotes normal
T6688 10768-10778 NN denotes morphology
T6689 10779-10780 -LRB- denotes (
T6690 10787-10789 NN denotes 5A
T6691 10780-10786 NN denotes Figure
T6692 10789-10790 -RRB- denotes )
T6693 10790-10791 . denotes .
T6694 10791-10914 sentence denotes These cells also proliferated at a speed comparable to that of the control (heterozygous and wild-type) cells (Figure 5B).
T6695 10792-10797 DT denotes These
T6696 10798-10803 NNS denotes cells
T6697 10809-10821 VBD denotes proliferated
T6698 10804-10808 RB denotes also
T6699 10822-10824 IN denotes at
T6700 10825-10826 DT denotes a
T6701 10827-10832 NN denotes speed
T6702 10833-10843 JJ denotes comparable
T6703 10844-10846 IN denotes to
T6704 10847-10851 DT denotes that
T6705 10852-10854 IN denotes of
T6706 10855-10858 DT denotes the
T6707 10896-10901 NNS denotes cells
T6708 10859-10866 NN denotes control
T6709 10867-10868 -LRB- denotes (
T6710 10868-10880 JJ denotes heterozygous
T6711 10881-10884 CC denotes and
T6712 10885-10889 JJ denotes wild
T6713 10890-10894 NN denotes type
T6714 10889-10890 HYPH denotes -
T6715 10894-10895 -RRB- denotes )
T6738 11002-11008 NN denotes Figure
T6739 11011-11012 -RRB- denotes )
T6740 11013-11015 CC denotes or
T6741 11016-11024 JJ denotes retinoic
T6742 11030-11039 NN denotes treatment
T6743 11025-11029 NN denotes acid
T6744 11040-11041 -LRB- denotes (
T6745 11045-11050 VBN denotes shown
T6746 11041-11044 RB denotes not
T6747 11050-11051 -RRB- denotes )
T6748 11051-11052 . denotes .
T6749 11052-11200 sentence denotes When transplanted into hind flanks of nude mice, these cells produced teratomas, tumors containing components of all three germ layers (Figure 5C).
T6750 11053-11057 WRB denotes When
T6751 11058-11070 VBN denotes transplanted
T6752 11114-11122 VBD denotes produced
T6753 11071-11075 IN denotes into
T6754 11076-11080 NN denotes hind
T6755 11081-11087 NNS denotes flanks
T6756 11088-11090 IN denotes of
T6757 11091-11095 JJ denotes nude
T6758 11096-11100 NNS denotes mice
T6759 11100-11102 , denotes ,
T6760 11102-11107 DT denotes these
T6761 11108-11113 NNS denotes cells
T6762 11123-11132 NNS denotes teratomas
T6763 11132-11134 , denotes ,
T6764 11134-11140 NNS denotes tumors
T6765 11141-11151 VBG denotes containing
T6766 11152-11162 NNS denotes components
T6767 11163-11165 IN denotes of
T6768 11166-11169 DT denotes all
T6769 11181-11187 NNS denotes layers
T6770 11170-11175 CD denotes three
T6771 11176-11180 NN denotes germ
T6772 11188-11189 -LRB- denotes (
T6773 11196-11198 NN denotes 5C
T6774 11189-11195 NN denotes Figure
T6775 11198-11199 -RRB- denotes )
T6776 11199-11200 . denotes .
T6777 11200-11310 sentence denotes These results indicate that ESG1 is dispensable for the self-renewal properties and pluripotency of ES cells.
T6778 11201-11206 DT denotes These
T6779 11207-11214 NNS denotes results
T6780 11215-11223 VBP denotes indicate
T6781 11224-11228 IN denotes that
T6782 11234-11236 VBZ denotes is
T6783 11229-11233 NN denotes ESG1
T6784 11237-11248 JJ denotes dispensable
T6785 11249-11252 IN denotes for
T6786 11253-11256 DT denotes the
T6787 11270-11280 NNS denotes properties
T6788 11257-11261 NN denotes self
T6789 11262-11269 NN denotes renewal
T6790 11261-11262 HYPH denotes -
T6791 11281-11284 CC denotes and
T6792 11285-11297 NN denotes pluripotency
T6793 11298-11300 IN denotes of
T6794 11301-11303 NN denotes ES
T6795 11304-11309 NNS denotes cells
T6796 11309-11310 . denotes .
T6797 11310-11311 sentence denotes
T6815 11886-11887 HYPH denotes /
T6816 11887-11888 SYM denotes -
T6817 11889-11891 NN denotes ES
T6818 11898-11903 VBG denotes using
T6819 11904-11919 NN denotes oligonucleotide
T6820 11920-11925 VBN denotes based
T6821 11919-11920 HYPH denotes -
T6822 11930-11941 NNS denotes microarrays
T6823 11926-11929 NN denotes DNA
T6824 11942-11954 VBG denotes representing
T6825 11955-11956 SYM denotes ~
T6826 11956-11962 CD denotes 20,000
T6827 11963-11968 NNS denotes genes
T6828 11968-11969 . denotes .
T6829 11969-12100 sentence denotes In comparison to control ES cells, ESG1 was identified as the gene reduced to the greatest extent in ESG1-/- ES cells (Figure 6A).
T6830 11970-11972 IN denotes In
T6831 12014-12024 VBN denotes identified
T6832 11973-11983 NN denotes comparison
T6833 11984-11986 IN denotes to
T6834 11987-11994 NN denotes control
T6835 11998-12003 NNS denotes cells
T6836 11995-11997 NN denotes ES
T6837 12003-12005 , denotes ,
T6838 12005-12009 NN denotes ESG1
T6839 12010-12013 VBD denotes was
T6840 12025-12027 IN denotes as
T6841 12028-12031 DT denotes the
T6842 12032-12036 NN denotes gene
T6843 12037-12044 VBN denotes reduced
T6844 12045-12047 IN denotes to
T6845 12048-12051 DT denotes the
T6846 12061-12067 NN denotes extent
T6847 12052-12060 JJS denotes greatest
T6848 12068-12070 IN denotes in
T6849 12071-12075 NN denotes ESG1
T6850 12082-12087 NNS denotes cells
T6851 12075-12076 SYM denotes -
T6852 12076-12077 HYPH denotes /
T6853 12077-12078 SYM denotes -
T6854 12079-12081 NN denotes ES
T6855 12088-12089 -LRB- denotes (
T6856 12096-12098 NN denotes 6A
T6857 12089-12095 NN denotes Figure
T6858 12098-12099 -RRB- denotes )
T6859 12099-12100 . denotes .
T6860 12100-12198 sentence denotes The expression of ES cell marker genes, such as Nanog and Oct3/4, was normal in ESG1-/- ES cells.
T6861 12101-12104 DT denotes The
T6862 12105-12115 NN denotes expression
T6863 12167-12170 VBD denotes was
T6864 12116-12118 IN denotes of
T6865 12119-12121 NN denotes ES
T6866 12122-12126 NN denotes cell
T6867 12134-12139 NNS denotes genes
T6868 12127-12133 NN denotes marker
T6869 12139-12141 , denotes ,
T6870 12141-12145 JJ denotes such
T6871 12146-12148 IN denotes as
T6872 12149-12154 NN denotes Nanog
T6873 12155-12158 CC denotes and
T6874 12159-12163 NN denotes Oct3
T6875 12163-12164 HYPH denotes /
T6876 12164-12165 CD denotes 4
T6877 12165-12167 , denotes ,
T6878 12171-12177 JJ denotes normal
T6879 12178-12180 IN denotes in
T6880 12181-12185 NN denotes ESG1
T6881 12192-12197 NNS denotes cells
T6882 12185-12186 SYM denotes -
T6883 12186-12187 HYPH denotes /
T6884 12187-12188 SYM denotes -
T6885 12189-12191 NN denotes ES
T6886 12197-12198 . denotes .
T6887 12198-12283 sentence denotes We confirmed normal Oct3/4 expression at protein levels by Western blot (Figure 6B).
T6888 12199-12201 PRP denotes We
T6889 12202-12211 VBD denotes confirmed
T6890 12212-12218 JJ denotes normal
T6891 12226-12236 NN denotes expression
T6892 12219-12223 NN denotes Oct3
T6893 12223-12224 HYPH denotes /
T6894 12224-12225 CD denotes 4
T6895 12237-12239 IN denotes at
T6897 12248-12254 NNS denotes levels
T6898 12255-12257 IN denotes by
T6899 12258-12265 NNP denotes Western
T6900 12266-12270 NN denotes blot
T6901 12271-12272 -LRB- denotes (
T6902 12279-12281 NN denotes 6B
T6903 12272-12278 NN denotes Figure
T6904 12281-12282 -RRB- denotes )
T6905 12282-12283 . denotes .
T6906 12283-12380 sentence denotes The overall gene expression profiles were similar between control ES cells and ESG1-/- ES cells.
T6907 12284-12287 DT denotes The
T6908 12312-12320 NNS denotes profiles
T6909 12288-12295 JJ denotes overall
T6910 12296-12300 NN denotes gene
T6911 12301-12311 NN denotes expression
T6912 12321-12325 VBD denotes were
T6913 12326-12333 JJ denotes similar
T6914 12334-12341 IN denotes between
T6915 12342-12349 NN denotes control
T6916 12353-12358 NNS denotes cells
T6917 12350-12352 NN denotes ES
T6918 12359-12362 CC denotes and
T6919 12363-12367 NN denotes ESG1
T6920 12374-12379 NNS denotes cells
T6921 12367-12368 SYM denotes -
T6922 12368-12369 HYPH denotes /
T6923 12369-12370 SYM denotes -
T6924 12371-12373 NN denotes ES
T6925 12379-12380 . denotes .
T6926 12380-12508 sentence denotes Several genes exhibited a greater than two-fold reduction in ESG1-/- cells, including Krt1-8, Pem, Ctgf, Ptgs2, Igf2 and Inhba.
T6927 12381-12388 JJ denotes Several
T6928 12389-12394 NNS denotes genes
T6929 12395-12404 VBD denotes exhibited
T6930 12405-12406 DT denotes a
T6931 12429-12438 NN denotes reduction
T6932 12407-12414 JJR denotes greater
T6933 12420-12428 JJ denotes two-fold
T6934 12415-12419 IN denotes than
T6935 12439-12441 IN denotes in
T6936 12442-12446 NN denotes ESG1
T6937 12450-12455 NNS denotes cells
T6938 12446-12447 SYM denotes -
T6939 12447-12448 HYPH denotes /
T6940 12448-12449 SYM denotes -
T6941 12455-12457 , denotes ,
T6942 12457-12466 VBG denotes including
T6943 12467-12471 NN denotes Krt1
T6944 12471-12472 HYPH denotes -
T6945 12472-12473 CD denotes 8
T6946 12473-12475 , denotes ,
T6947 12475-12478 NN denotes Pem
T6948 12478-12480 , denotes ,
T6949 12480-12484 NN denotes Ctgf
T6950 12484-12486 , denotes ,
T6951 12486-12491 NN denotes Ptgs2
T6952 12491-12493 , denotes ,
T6953 12493-12497 NN denotes Igf2
T6954 12498-12501 CC denotes and
T6955 12502-12507 NN denotes Inhba
T6956 12507-12508 . denotes .
T6957 12508-12571 sentence denotes These genes might be regulated directly or indirectly by ESG1.
T6958 12509-12514 DT denotes These
T6959 12515-12520 NNS denotes genes
T6960 12530-12539 VBN denotes regulated
T6961 12521-12526 MD denotes might
T6962 12527-12529 VB denotes be
T6963 12540-12548 RB denotes directly
T6964 12549-12551 CC denotes or
T6965 12552-12562 RB denotes indirectly
T6966 12563-12565 IN denotes by
T6967 12566-12570 NN denotes ESG1
T6968 12570-12571 . denotes .
T6969 12571-12659 sentence denotes Since ESG1 contains a KH-type RNA-binding domain, it may stabilize mRNA of these genes.
T6970 12572-12577 IN denotes Since
T6971 12583-12591 VBZ denotes contains
T6972 12578-12582 NN denotes ESG1
T6973 12629-12638 VB denotes stabilize
T6974 12592-12593 DT denotes a
T6975 12614-12620 NN denotes domain
T6976 12594-12596 NN denotes KH
T6977 12597-12601 NN denotes type
T6978 12596-12597 HYPH denotes -
T6979 12602-12605 NN denotes RNA
T6980 12606-12613 VBG denotes binding
T6981 12605-12606 HYPH denotes -
T6982 12620-12622 , denotes ,
T6983 12622-12624 PRP denotes it
T6984 12625-12628 MD denotes may
T6985 12639-12643 NN denotes mRNA
T6986 12644-12646 IN denotes of
T6987 12647-12652 DT denotes these
T6988 12653-12658 NNS denotes genes
T6989 12658-12659 . denotes .
T6990 12659-12717 sentence denotes Further studied are required to clarify this possibility.
T6991 12660-12667 RB denotes Further
T6992 12668-12675 VBN denotes studied
T6993 12680-12688 VBN denotes required
T6994 12676-12679 VBP denotes are
T6995 12689-12691 TO denotes to
T6996 12692-12699 VB denotes clarify
T6997 12700-12704 DT denotes this
T6998 12705-12716 NN denotes possibility
T6999 12716-12717 . denotes .
T7000 12717-12718 sentence denotes
T7017 13199-13201 , denotes ,
T7018 13201-13203 PRP denotes we
T7019 13213-13214 NN denotes β
T7020 13219-13223 NN denotes ESG1
T7021 13214-13215 HYPH denotes -
T7022 13215-13218 NN denotes geo
T7023 13218-13219 HYPH denotes -
T7024 13235-13241 NNS denotes clones
T7025 13223-13224 SYM denotes +
T7026 13224-13225 HYPH denotes /
T7027 13225-13226 SYM denotes -
T7028 13227-13229 NN denotes ES
T7029 13230-13234 NN denotes cell
T7030 13242-13246 IN denotes into
T7031 13247-13250 DT denotes the
T7032 13251-13262 NNS denotes blastocysts
T7033 13263-13265 IN denotes of
T7034 13266-13272 NN denotes C57BL6
T7035 13273-13277 NNS denotes mice
T7036 13277-13278 . denotes .
T7037 13278-13331 sentence denotes We obtained germline transmission from three clones.
T7038 13279-13281 PRP denotes We
T7039 13282-13290 VBD denotes obtained
T7040 13291-13299 NN denotes germline
T7041 13300-13312 NN denotes transmission
T7042 13313-13317 IN denotes from
T7043 13318-13323 CD denotes three
T7044 13324-13330 NNS denotes clones
T7045 13330-13331 . denotes .
T7046 13331-13458 sentence denotes We obtained ESG1-/- mice at the Mendelian ratios (36 wild-type, 69 ESG1+/-, and 45 ESG1-/-) from intercrosses of ESG1+/- mice.
T7047 13332-13334 PRP denotes We
T7048 13335-13343 VBD denotes obtained
T7049 13344-13348 NN denotes ESG1
T7050 13352-13356 NNS denotes mice
T7051 13348-13349 SYM denotes -
T7052 13349-13350 HYPH denotes /
T7053 13350-13351 SYM denotes -
T7054 13357-13359 IN denotes at
T7055 13360-13363 DT denotes the
T7056 13374-13380 NNS denotes ratios
T7057 13364-13373 JJ denotes Mendelian
T7058 13381-13382 -LRB- denotes (
T7059 13382-13384 CD denotes 36
T7060 13390-13394 NN denotes type
T7061 13385-13389 JJ denotes wild
T7062 13389-13390 HYPH denotes -
T7063 13394-13396 , denotes ,
T7064 13396-13398 CD denotes 69
T7065 13399-13403 NN denotes ESG1
T7066 13403-13404 SYM denotes +
T7067 13404-13405 HYPH denotes /
T7068 13405-13406 SYM denotes -
T7069 13406-13408 , denotes ,
T7070 13408-13411 CC denotes and
T7071 13412-13414 CD denotes 45
T7072 13415-13419 NN denotes ESG1
T7073 13419-13420 SYM denotes -
T7074 13420-13421 HYPH denotes /
T7075 13421-13422 SYM denotes -
T7076 13422-13423 -RRB- denotes )
T7077 13424-13428 IN denotes from
T7078 13429-13441 NNS denotes intercrosses
T7079 13442-13444 IN denotes of
T7080 13445-13449 NN denotes ESG1
T7081 13453-13457 NNS denotes mice
T7082 13449-13450 SYM denotes +
T7083 13450-13451 HYPH denotes /
T7084 13451-13452 SYM denotes -
T7085 13457-13458 . denotes .
T7086 13458-13547 sentence denotes These animals exhibited normal development, gross appearance, and fertility (not shown).
T7087 13459-13464 DT denotes These
T7088 13465-13472 NNS denotes animals
T7089 13473-13482 VBD denotes exhibited
T7090 13483-13489 JJ denotes normal
T7091 13490-13501 NN denotes development
T7092 13501-13503 , denotes ,
T7093 13503-13508 JJ denotes gross
T7094 13509-13519 NN denotes appearance
T7095 13519-13521 , denotes ,
T7096 13521-13524 CC denotes and
T7097 13525-13534 NN denotes fertility
T7098 13535-13536 -LRB- denotes (
T7099 13540-13545 VBN denotes shown
T7100 13536-13539 RB denotes not
T7101 13545-13546 -RRB- denotes )
T7102 13546-13547 . denotes .
T7103 13547-13642 sentence denotes Histological examination of testis and ovary could not identify any abnormalities (not shown).
T7104 13548-13560 JJ denotes Histological
T7105 13561-13572 NN denotes examination
T7106 13603-13611 VB denotes identify
T7107 13573-13575 IN denotes of
T7108 13576-13582 NN denotes testis
T7109 13583-13586 CC denotes and
T7110 13587-13592 NN denotes ovary
T7111 13593-13598 MD denotes could
T7112 13599-13602 RB denotes not
T7113 13612-13615 DT denotes any
T7114 13616-13629 NNS denotes abnormalities
T7115 13630-13631 -LRB- denotes (
T7116 13635-13640 VBN denotes shown
T7117 13631-13634 RB denotes not
T7118 13640-13641 -RRB- denotes )
T7119 13641-13642 . denotes .
T7120 13642-13743 sentence denotes These data demonstrated that ESG1 is dispensable for both mouse development and germ cell formation.
T7121 13643-13648 DT denotes These
T7122 13649-13653 NNS denotes data
T7123 13654-13666 VBD denotes demonstrated
T7124 13667-13671 IN denotes that
T7125 13677-13679 VBZ denotes is
T7126 13672-13676 NN denotes ESG1
T7127 13680-13691 JJ denotes dispensable
T7128 13692-13695 IN denotes for
T7129 13696-13700 CC denotes both
T7130 13707-13718 NN denotes development
T7131 13701-13706 NN denotes mouse
T7132 13719-13722 CC denotes and
T7133 13723-13727 NN denotes germ
T7134 13728-13732 NN denotes cell
T7135 13733-13742 NN denotes formation
T7136 13742-13743 . denotes .
T7137 13743-13850 sentence denotes We also generated ES cells from blastocysts obtained by intercrosses of ESG1+/- males and ESG1-/- females.
T7138 13744-13746 PRP denotes We
T7139 13752-13761 VBD denotes generated
T7140 13747-13751 RB denotes also
T7141 13762-13764 NN denotes ES
T7142 13765-13770 NNS denotes cells
T7143 13771-13775 IN denotes from
T7144 13776-13787 NNS denotes blastocysts
T7145 13788-13796 VBN denotes obtained
T7146 13797-13799 IN denotes by
T7147 13800-13812 NNS denotes intercrosses
T7148 13813-13815 IN denotes of
T7149 13816-13820 NN denotes ESG1
T7150 13824-13829 NNS denotes males
T7151 13820-13821 SYM denotes +
T7152 13821-13822 HYPH denotes /
T7153 13822-13823 SYM denotes -
T7154 13830-13833 CC denotes and
T7155 13834-13838 NN denotes ESG1
T7156 13842-13849 NNS denotes females
T7157 13838-13839 SYM denotes -
T7158 13839-13840 HYPH denotes /
T7159 13840-13841 SYM denotes -
T7160 13849-13850 . denotes .
T7161 13850-13915 sentence denotes Of the eight ES cell lines established, two clones were ESG1-/-.
T7162 13851-13853 IN denotes Of
T7163 13902-13906 VBD denotes were
T7164 13854-13857 DT denotes the
T7165 13872-13877 NNS denotes lines
T7166 13858-13863 CD denotes eight
T7167 13864-13866 NN denotes ES
T7168 13867-13871 NN denotes cell
T7169 13878-13889 VBN denotes established
T7170 13889-13891 , denotes ,
T7171 13891-13894 CD denotes two
T7172 13895-13901 NNS denotes clones
T7173 13907-13911 NN denotes ESG1
T7174 13911-13912 SYM denotes -
T7175 13912-13913 HYPH denotes /
T7176 13913-13914 SYM denotes -
T7177 13914-13915 . denotes .
T7178 13915-14069 sentence denotes These ESG1-null ES cells demonstrated normal morphology, proliferation, and differentiation (not shown), confirming that ESG1 is dispensable in ES cells.
T7179 13916-13921 DT denotes These
T7180 13935-13940 NNS denotes cells
T7181 13922-13926 NN denotes ESG1
T7182 13927-13931 JJ denotes null
T7183 13926-13927 HYPH denotes -
T7184 13932-13934 NN denotes ES
T7185 13941-13953 VBD denotes demonstrated
T7186 13954-13960 JJ denotes normal
T7187 13961-13971 NN denotes morphology
T7188 13971-13973 , denotes ,
T7189 13973-13986 NN denotes proliferation
T7190 13986-13988 , denotes ,
T7191 13988-13991 CC denotes and
T7192 13992-14007 NN denotes differentiation
T7193 14008-14009 -LRB- denotes (
T7194 14013-14018 VBN denotes shown
T7195 14009-14012 RB denotes not
T7196 14018-14019 -RRB- denotes )
T7197 14019-14021 , denotes ,
T7198 14021-14031 VBG denotes confirming
T7199 14032-14036 IN denotes that
T7200 14042-14044 VBZ denotes is
T7201 14037-14041 NN denotes ESG1
T7202 14045-14056 JJ denotes dispensable
T7203 14057-14059 IN denotes in
T7204 14060-14062 NN denotes ES
T7205 14063-14068 NNS denotes cells
T7206 14068-14069 . denotes .
T7369 14082-14084 TO denotes To
T7370 14085-14092 VB denotes analyze
T7371 14129-14139 VBD denotes identified
T7372 14093-14096 DT denotes the
T7373 14111-14116 NNS denotes roles
T7374 14097-14110 JJ denotes physiological
T7375 14117-14119 IN denotes of
T7376 14120-14124 NN denotes ESG1
T7377 14124-14126 , denotes ,
T7378 14126-14128 PRP denotes we
T7379 14140-14143 DT denotes the
T7380 14150-14154 NN denotes gene
T7381 14144-14149 NN denotes mouse
T7382 14155-14157 IN denotes on
T7383 14158-14168 NN denotes chromosome
T7384 14169-14170 CD denotes 9
T7385 14171-14174 CC denotes and
T7386 14175-14182 VBD denotes deleted
T7387 14183-14185 PRP denotes it
T7388 14186-14188 IN denotes by
T7389 14189-14199 JJ denotes homologous
T7390 14200-14213 NN denotes recombination
T7391 14214-14216 IN denotes in
T7392 14217-14219 NN denotes ES
T7393 14220-14225 NNS denotes cells
T7394 14225-14226 . denotes .
T7395 14226-14424 sentence denotes Despite specific expression in early embryos, germ cells, and pluripotent cells, our data demonstrated that ESG1 is dispensable for mouse development, germ cell formation, and ES cell self-renewal.
T7396 14227-14234 IN denotes Despite
T7397 14317-14329 VBD denotes demonstrated
T7398 14235-14243 JJ denotes specific
T7399 14244-14254 NN denotes expression
T7400 14255-14257 IN denotes in
T7401 14258-14263 JJ denotes early
T7402 14264-14271 NNS denotes embryos
T7403 14271-14273 , denotes ,
T7404 14273-14277 NN denotes germ
T7405 14278-14283 NNS denotes cells
T7406 14283-14285 , denotes ,
T7407 14285-14288 CC denotes and
T7408 14289-14300 JJ denotes pluripotent
T7409 14301-14306 NNS denotes cells
T7410 14306-14308 , denotes ,
T7411 14308-14311 PRP$ denotes our
T7412 14312-14316 NNS denotes data
T7413 14330-14334 IN denotes that
T7414 14340-14342 VBZ denotes is
T7415 14335-14339 NN denotes ESG1
T7416 14343-14354 JJ denotes dispensable
T7417 14355-14358 IN denotes for
T7418 14359-14364 NN denotes mouse
T7419 14365-14376 NN denotes development
T7420 14376-14378 , denotes ,
T7421 14378-14382 NN denotes germ
T7422 14383-14387 NN denotes cell
T7423 14388-14397 NN denotes formation
T7424 14397-14399 , denotes ,
T7425 14399-14402 CC denotes and
T7426 14403-14405 NN denotes ES
T7427 14406-14410 NN denotes cell
T7428 14416-14423 NN denotes renewal
T7429 14411-14415 NN denotes self
T7430 14415-14416 HYPH denotes -
T7431 14423-14424 . denotes .
T8264 14435-14449 NN denotes Identification
T8265 14450-14453 CC denotes and
T8266 14454-14462 NNS denotes analyses
T8267 14463-14465 IN denotes of
T8268 14466-14469 NN denotes BAC
T8269 14470-14476 NNS denotes clones
T8270 14477-14487 VBG denotes containing
T8271 14488-14491 DT denotes the
T8272 14503-14507 NN denotes gene
T8273 14492-14497 NN denotes mouse
T8274 14498-14502 NN denotes ESG1
T8275 14507-14777 sentence denotes To identify bacterial artificial chromosome (BAC) clones containing mouse ESG1 gene, we performed PCR-based screening of mouse BAC library DNA pools (Research Genetics) using the pH34-u38 (5'-GAAGTCTGGTTCCTTGGCAGG-3') and pH34-L394 (5'-ACTCGATACACTGGCCTAGC-3') primers.
T8276 14508-14510 TO denotes To
T8277 14511-14519 VB denotes identify
T8278 14596-14605 VBD denotes performed
T8279 14520-14529 JJ denotes bacterial
T8280 14541-14551 NN denotes chromosome
T8281 14530-14540 JJ denotes artificial
T8282 14558-14564 NNS denotes clones
T8283 14552-14553 -LRB- denotes (
T8284 14553-14556 NN denotes BAC
T8285 14556-14557 -RRB- denotes )
T8286 14565-14575 VBG denotes containing
T8287 14576-14581 NN denotes mouse
T8288 14587-14591 NN denotes gene
T8289 14582-14586 NN denotes ESG1
T8290 14591-14593 , denotes ,
T8291 14593-14595 PRP denotes we
T8292 14606-14609 NN denotes PCR
T8293 14610-14615 VBN denotes based
T8294 14609-14610 HYPH denotes -
T8295 14616-14625 NN denotes screening
T8296 14626-14628 IN denotes of
T8297 14629-14634 NN denotes mouse
T8298 14651-14656 NNS denotes pools
T8299 14635-14638 NN denotes BAC
T8300 14639-14646 NN denotes library
T8301 14647-14650 NN denotes DNA
T8302 14657-14658 -LRB- denotes (
T8303 14667-14675 NNP denotes Genetics
T8304 14658-14666 NNP denotes Research
T8305 14675-14676 -RRB- denotes )
T8306 14677-14682 VBG denotes using
T8307 14683-14686 DT denotes the
T8308 14769-14776 NNS denotes primers
T8309 14687-14691 NN denotes pH34
T8310 14692-14695 NN denotes u38
T8311 14691-14692 HYPH denotes -
T8312 14696-14697 -LRB- denotes (
T8313 14722-14723 CD denotes 3
T8314 14697-14698 CD denotes 5
T8315 14698-14699 SYM denotes '
T8316 14699-14700 HYPH denotes -
T8317 14700-14721 NN denotes GAAGTCTGGTTCCTTGGCAGG
T8318 14721-14722 HYPH denotes -
T8319 14723-14724 SYM denotes '
T8320 14724-14725 -RRB- denotes )
T8321 14726-14729 CC denotes and
T8322 14730-14734 NN denotes pH34
T8323 14735-14739 NN denotes L394
T8324 14734-14735 HYPH denotes -
T8325 14740-14741 -LRB- denotes (
T8326 14765-14766 CD denotes 3
T8327 14741-14742 CD denotes 5
T8328 14742-14743 SYM denotes '
T8329 14743-14744 HYPH denotes -
T8330 14744-14764 NN denotes ACTCGATACACTGGCCTAGC
T8331 14764-14765 HYPH denotes -
T8332 14766-14767 SYM denotes '
T8333 14767-14768 -RRB- denotes )
T8334 14776-14777 . denotes .
T8335 14777-15133 sentence denotes Following restriction enzyme digestion, we performed Southern blot analyses of BAC clones as described [20] using the pH34-U258 (5'-CTCGAGTGTACAGTCAAGTGGTTGCTGGGA-3'), pH34-U65 (5'-GTGACCCTCGTGACCCGTAA-3'), pH34-intron1L (5'-CTGCGTGAGAGAAACACCAAACAGGC-3'), pH34-L545 (5'-TGTGAATGGGAAGGTTACCACTCT-3') and pH34-SCL1 (5'-GCCCTCTTCTGGTTTGTCTCGAAAT-3') probes.
T8336 14778-14787 VBG denotes Following
T8337 14821-14830 VBD denotes performed
T8338 14788-14799 NN denotes restriction
T8339 14800-14806 NN denotes enzyme
T8340 14807-14816 NN denotes digestion
T8341 14816-14818 , denotes ,
T8342 14818-14820 PRP denotes we
T8343 14831-14839 NNP denotes Southern
T8344 14840-14844 NN denotes blot
T8345 14845-14853 NNS denotes analyses
T8346 14854-14856 IN denotes of
T8347 14857-14860 NN denotes BAC
T8348 14861-14867 NNS denotes clones
T8349 14868-14870 IN denotes as
T8350 14871-14880 VBN denotes described
T8351 14881-14882 -LRB- denotes [
T8352 14882-14884 CD denotes 20
T8353 14884-14885 -RRB- denotes ]
T8354 14886-14891 VBG denotes using
T8355 14892-14895 DT denotes the
T8356 15126-15132 NNS denotes probes
T8357 14896-14900 NN denotes pH34
T8358 14901-14905 NN denotes U258
T8359 14900-14901 HYPH denotes -
T8360 14906-14907 -LRB- denotes (
T8361 14910-14940 NN denotes CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
T8362 14907-14908 CD denotes 5
T8363 14908-14909 SYM denotes '
T8364 14909-14910 HYPH denotes -
T8365 14940-14941 HYPH denotes -
T8366 14941-14942 CD denotes 3
T8367 14942-14943 SYM denotes '
T8368 14943-14944 -RRB- denotes )
T8369 14944-14946 , denotes ,
T8370 14946-14950 NN denotes pH34
T8371 14951-14954 NN denotes U65
T8372 14950-14951 HYPH denotes -
T8373 14955-14956 -LRB- denotes (
T8374 14959-14979 NN denotes GTGACCCTCGTGACCCGTAA
T8375 14956-14957 CD denotes 5
T8376 14957-14958 SYM denotes '
T8377 14958-14959 HYPH denotes -
T8378 14979-14980 HYPH denotes -
T8379 14980-14981 CD denotes 3
T8380 14981-14982 SYM denotes '
T8381 14982-14983 -RRB- denotes )
T8382 14983-14985 , denotes ,
T8383 14985-14989 NN denotes pH34
T8384 14990-14998 NN denotes intron1L
T8385 14989-14990 HYPH denotes -
T8386 14999-15000 -LRB- denotes (
T8387 15003-15029 NN denotes CTGCGTGAGAGAAACACCAAACAGGC
T8388 15000-15001 CD denotes 5
T8389 15001-15002 SYM denotes '
T8390 15002-15003 HYPH denotes -
T8391 15029-15030 HYPH denotes -
T8392 15030-15031 CD denotes 3
T8393 15031-15032 SYM denotes '
T8394 15032-15033 -RRB- denotes )
T8395 15033-15035 , denotes ,
T8396 15035-15039 NN denotes pH34
T8397 15040-15044 NN denotes L545
T8398 15039-15040 HYPH denotes -
T8399 15045-15046 -LRB- denotes (
T8400 15049-15073 NN denotes TGTGAATGGGAAGGTTACCACTCT
T8401 15046-15047 CD denotes 5
T8402 15047-15048 SYM denotes '
T8403 15048-15049 HYPH denotes -
T8404 15073-15074 HYPH denotes -
T8405 15074-15075 CD denotes 3
T8406 15075-15076 SYM denotes '
T8407 15076-15077 -RRB- denotes )
T8408 15078-15081 CC denotes and
T8409 15082-15086 NN denotes pH34
T8410 15087-15091 NN denotes SCL1
T8411 15086-15087 HYPH denotes -
T8412 15092-15093 -LRB- denotes (
T8413 15096-15121 NN denotes GCCCTCTTCTGGTTTGTCTCGAAAT
T8414 15093-15094 CD denotes 5
T8415 15094-15095 SYM denotes '
T8416 15095-15096 HYPH denotes -
T8417 15121-15122 HYPH denotes -
T8418 15122-15123 CD denotes 3
T8419 15123-15124 SYM denotes '
T8420 15124-15125 -RRB- denotes )
T8421 15132-15133 . denotes .
T8422 15133-15228 sentence denotes Hybridization with these probes revealed bands containing either the ESG1 gene or pseudogenes.
T8423 15134-15147 NN denotes Hybridization
T8424 15166-15174 VBD denotes revealed
T8425 15148-15152 IN denotes with
T8426 15153-15158 DT denotes these
T8427 15159-15165 NNS denotes probes
T8428 15175-15180 NNS denotes bands
T8429 15181-15191 VBG denotes containing
T8430 15192-15198 CC denotes either
T8431 15208-15212 NN denotes gene
T8432 15199-15202 DT denotes the
T8433 15203-15207 NN denotes ESG1
T8434 15213-15215 CC denotes or
T8435 15216-15227 NNS denotes pseudogenes
T8436 15227-15228 . denotes .
T8437 15228-15390 sentence denotes To sequence the region containing the mouse ESG1 gene and the 3' flanking region, we subcloned a ~15 kbp XhoI-SalI fragment into the pZERO-2 vector (Invitrogen).
T8438 15229-15231 TO denotes To
T8439 15232-15240 VB denotes sequence
T8440 15314-15323 VBD denotes subcloned
T8441 15241-15244 DT denotes the
T8442 15245-15251 NN denotes region
T8443 15252-15262 VBG denotes containing
T8444 15263-15266 DT denotes the
T8445 15278-15282 NN denotes gene
T8446 15267-15272 NN denotes mouse
T8447 15273-15277 NN denotes ESG1
T8448 15283-15286 CC denotes and
T8449 15287-15290 DT denotes the
T8450 15303-15309 NN denotes region
T8451 15291-15292 CD denotes 3
T8452 15292-15293 SYM denotes '
T8453 15294-15302 NN denotes flanking
T8454 15309-15311 , denotes ,
T8455 15311-15313 PRP denotes we
T8456 15324-15325 DT denotes a
T8457 15344-15352 NN denotes fragment
T8458 15326-15327 SYM denotes ~
T8459 15327-15329 CD denotes 15
T8460 15330-15333 NN denotes kbp
T8461 15334-15338 NN denotes XhoI
T8462 15339-15343 NN denotes SalI
T8463 15338-15339 HYPH denotes -
T8464 15353-15357 IN denotes into
T8465 15358-15361 DT denotes the
T8466 15370-15376 NN denotes vector
T8467 15362-15367 NN denotes pZERO
T8468 15367-15368 HYPH denotes -
T8469 15368-15369 CD denotes 2
T8470 15377-15378 -LRB- denotes (
T8471 15378-15388 NNP denotes Invitrogen
T8472 15388-15389 -RRB- denotes )
T8473 15389-15390 . denotes .
T8474 15390-15498 sentence denotes HindIII- or EcoRI-digested fragments of this vector were then cloned into pBluescript KS(-) for sequencing.
T8475 15391-15398 NN denotes HindIII
T8476 15409-15417 VBN denotes digested
T8477 15398-15399 HYPH denotes -
T8478 15400-15402 CC denotes or
T8479 15403-15408 NN denotes EcoRI
T8480 15408-15409 HYPH denotes -
T8481 15418-15427 NNS denotes fragments
T8482 15453-15459 VBN denotes cloned
T8483 15428-15430 IN denotes of
T8484 15431-15435 DT denotes this
T8485 15436-15442 NN denotes vector
T8486 15443-15447 VBD denotes were
T8487 15448-15452 RB denotes then
T8488 15460-15464 IN denotes into
T8489 15465-15476 NN denotes pBluescript
T8490 15477-15479 NN denotes KS
T8491 15479-15480 -LRB- denotes (
T8492 15480-15481 SYM denotes -
T8493 15481-15482 -RRB- denotes )
T8494 15483-15486 IN denotes for
T8495 15487-15497 NN denotes sequencing
T8496 15497-15498 . denotes .
T8497 15498-15621 sentence denotes To sequence the ESG1 pseudogene and the 3' flanking region, an 8 kbp NotI/XhoI fragment was cloned into pBluescript KS(-).
T8498 15499-15501 TO denotes To
T8499 15502-15510 VB denotes sequence
T8500 15591-15597 VBN denotes cloned
T8501 15511-15514 DT denotes the
T8502 15520-15530 NN denotes pseudogene
T8503 15515-15519 NN denotes ESG1
T8504 15531-15534 CC denotes and
T8505 15535-15538 DT denotes the
T8506 15551-15557 NN denotes region
T8507 15539-15540 CD denotes 3
T8508 15540-15541 SYM denotes '
T8509 15542-15550 NN denotes flanking
T8510 15557-15559 , denotes ,
T8511 15559-15561 DT denotes an
T8512 15578-15586 NN denotes fragment
T8513 15562-15563 CD denotes 8
T8514 15564-15567 NN denotes kbp
T8515 15568-15572 NN denotes NotI
T8516 15573-15577 NN denotes XhoI
T8517 15572-15573 HYPH denotes /
T8518 15587-15590 VBD denotes was
T8519 15598-15602 IN denotes into
T8520 15603-15614 NN denotes pBluescript
T8521 15615-15617 NN denotes KS
T8522 15617-15618 -LRB- denotes (
T8523 15618-15619 SYM denotes -
T8524 15619-15620 -RRB- denotes )
T8525 15620-15621 . denotes .
T8526 15621-15703 sentence denotes BamHI- or PstI- fragments of this vector were also cloned into pBluescript KS(-).
T8527 15622-15627 NN denotes BamHI
T8528 15638-15647 NNS denotes fragments
T8529 15627-15628 SYM denotes -
T8530 15629-15631 CC denotes or
T8531 15632-15636 NN denotes PstI
T8532 15636-15637 SYM denotes -
T8533 15673-15679 VBN denotes cloned
T8534 15648-15650 IN denotes of
T8535 15651-15655 DT denotes this
T8536 15656-15662 NN denotes vector
T8537 15663-15667 VBD denotes were
T8538 15668-15672 RB denotes also
T8539 15680-15684 IN denotes into
T8540 15685-15696 NN denotes pBluescript
T8541 15697-15699 NN denotes KS
T8542 15699-15700 -LRB- denotes (
T8543 15700-15701 SYM denotes -
T8544 15701-15702 -RRB- denotes )
T8545 15702-15703 . denotes .
T8546 15703-15928 sentence denotes To identify the sequence containing the 5' flanking regions of the ESG1 gene and the related pseudogenes, we used a TOPO walker kit (Invitrogen) with the pH34-T2L (5'-ACTAGTCGCAGCAGGGATCCAGGAATATCT-3') and pH34-L394 primers.
T8547 15704-15706 TO denotes To
T8548 15707-15715 VB denotes identify
T8549 15813-15817 VBD denotes used
T8550 15716-15719 DT denotes the
T8551 15720-15728 NN denotes sequence
T8552 15729-15739 VBG denotes containing
T8553 15740-15743 DT denotes the
T8554 15756-15763 NNS denotes regions
T8555 15744-15745 CD denotes 5
T8556 15745-15746 SYM denotes '
T8557 15747-15755 NN denotes flanking
T8558 15764-15766 IN denotes of
T8559 15767-15770 DT denotes the
T8560 15776-15780 NN denotes gene
T8561 15771-15775 NN denotes ESG1
T8562 15781-15784 CC denotes and
T8563 15785-15788 DT denotes the
T8564 15797-15808 NNS denotes pseudogenes
T8565 15789-15796 JJ denotes related
T8566 15808-15810 , denotes ,
T8567 15810-15812 PRP denotes we
T8568 15818-15819 DT denotes a
T8569 15832-15835 NN denotes kit
T8570 15820-15824 NN denotes TOPO
T8571 15825-15831 NN denotes walker
T8572 15836-15837 -LRB- denotes (
T8573 15837-15847 NNP denotes Invitrogen
T8574 15847-15848 -RRB- denotes )
T8575 15849-15853 IN denotes with
T8576 15854-15857 DT denotes the
T8577 15920-15927 NNS denotes primers
T8578 15858-15862 NN denotes pH34
T8579 15863-15866 NN denotes T2L
T8580 15862-15863 HYPH denotes -
T8581 15867-15868 -LRB- denotes (
T8582 15902-15903 CD denotes 3
T8583 15868-15869 CD denotes 5
T8584 15869-15870 SYM denotes '
T8585 15870-15871 HYPH denotes -
T8586 15871-15901 NN denotes ACTAGTCGCAGCAGGGATCCAGGAATATCT
T8587 15901-15902 HYPH denotes -
T8588 15903-15904 SYM denotes '
T8589 15904-15905 -RRB- denotes )
T8590 15906-15909 CC denotes and
T8591 15910-15914 NN denotes pH34
T8592 15915-15919 NN denotes L394
T8593 15914-15915 HYPH denotes -
T8594 15927-15928 . denotes .
T8595 15928-15988 sentence denotes The resulting sequence was cloned into pCR2.1 (Invitrogen).
T8596 15929-15932 DT denotes The
T8597 15943-15951 NN denotes sequence
T8598 15933-15942 VBG denotes resulting
T8599 15956-15962 VBN denotes cloned
T8600 15952-15955 VBD denotes was
T8601 15963-15967 IN denotes into
T8602 15968-15974 NN denotes pCR2.1
T8603 15975-15976 -LRB- denotes (
T8604 15976-15986 NNP denotes Invitrogen
T8605 15986-15987 -RRB- denotes )
T8606 15987-15988 . denotes .
T8607 15988-16161 sentence denotes We obtained a ~6 kbp band from the NsiI-digensted library; XbaI-, SpeI-, EcoRI-, and PstI-digested fragments of this band were cloned into pBluescript KS(-) for sequencing.
T8608 15989-15991 PRP denotes We
T8609 15992-16000 VBD denotes obtained
T8610 16116-16122 VBN denotes cloned
T8611 16001-16002 DT denotes a
T8612 16010-16014 NN denotes band
T8613 16003-16004 SYM denotes ~
T8614 16004-16005 CD denotes 6
T8615 16006-16009 NN denotes kbp
T8616 16015-16019 IN denotes from
T8617 16020-16023 DT denotes the
T8618 16039-16046 NN denotes library
T8619 16024-16028 NN denotes NsiI
T8620 16029-16038 VBN denotes digensted
T8621 16028-16029 HYPH denotes -
T8622 16046-16047 : denotes ;
T8623 16048-16052 NN denotes XbaI
T8624 16079-16087 VBN denotes digested
T8625 16052-16053 HYPH denotes -
T8626 16053-16055 , denotes ,
T8627 16055-16059 NN denotes SpeI
T8628 16059-16060 HYPH denotes -
T8629 16060-16062 , denotes ,
T8630 16062-16067 NN denotes EcoRI
T8631 16067-16068 HYPH denotes -
T8632 16068-16070 , denotes ,
T8633 16070-16073 CC denotes and
T8634 16074-16078 NN denotes PstI
T8635 16078-16079 HYPH denotes -
T8636 16088-16097 NNS denotes fragments
T8637 16098-16100 IN denotes of
T8638 16101-16105 DT denotes this
T8639 16106-16110 NN denotes band
T8640 16111-16115 VBD denotes were
T8641 16123-16127 IN denotes into
T8642 16128-16139 NN denotes pBluescript
T8643 16140-16142 NN denotes KS
T8644 16142-16143 -LRB- denotes (
T8645 16143-16144 SYM denotes -
T8646 16144-16145 -RRB- denotes )
T8647 16146-16149 IN denotes for
T8648 16150-16160 NN denotes sequencing
T8649 16160-16161 . denotes .
T8650 16161-16226 sentence denotes This fragment contained the 5' flanking region of the ESG1 gene.
T8651 16162-16166 DT denotes This
T8652 16167-16175 NN denotes fragment
T8653 16176-16185 VBD denotes contained
T8654 16186-16189 DT denotes the
T8655 16202-16208 NN denotes region
T8656 16190-16191 CD denotes 5
T8657 16191-16192 SYM denotes '
T8658 16193-16201 NN denotes flanking
T8659 16209-16211 IN denotes of
T8660 16212-16215 DT denotes the
T8661 16221-16225 NN denotes gene
T8662 16216-16220 NN denotes ESG1
T8663 16225-16226 . denotes .
T8664 16226-16325 sentence denotes A ~3 kbp fragment, obtained from the SacI-digested library, was cloned into pCR2.1 for sequencing.
T8665 16227-16228 DT denotes A
T8666 16236-16244 NN denotes fragment
T8667 16229-16230 SYM denotes ~
T8668 16230-16231 CD denotes 3
T8669 16232-16235 NN denotes kbp
T8670 16291-16297 VBN denotes cloned
T8671 16244-16246 , denotes ,
T8672 16246-16254 VBN denotes obtained
T8673 16255-16259 IN denotes from
T8674 16260-16263 DT denotes the
T8675 16278-16285 NN denotes library
T8676 16264-16268 NN denotes SacI
T8677 16269-16277 VBN denotes digested
T8678 16268-16269 HYPH denotes -
T8679 16285-16287 , denotes ,
T8680 16287-16290 VBD denotes was
T8681 16298-16302 IN denotes into
T8682 16303-16309 NN denotes pCR2.1
T8683 16310-16313 IN denotes for
T8684 16314-16324 NN denotes sequencing
T8685 16324-16325 . denotes .
T8686 16325-16392 sentence denotes This fragment was contained the 5' region flanking the pseudogene.
T8687 16326-16330 DT denotes This
T8688 16331-16339 NN denotes fragment
T8689 16344-16353 VBN denotes contained
T8690 16340-16343 VBD denotes was
T8691 16354-16357 DT denotes the
T8692 16361-16367 NN denotes region
T8693 16358-16359 CD denotes 5
T8694 16359-16360 SYM denotes '
T8695 16368-16376 VBG denotes flanking
T8696 16377-16380 DT denotes the
T8697 16381-16391 NN denotes pseudogene
T8698 16391-16392 . denotes .
T9171 16394-16406 NN denotes Construction
T9172 16407-16409 IN denotes of
T9173 16410-16414 NN denotes ESG1
T9174 16425-16432 NNS denotes vectors
T9175 16415-16424 NN denotes targeting
T9176 16432-16592 sentence denotes We replaced all of the ESG1 exons with two targeting vectors containing either an IRES-β-geo cassette [21] or an IRES-HygR cassette by promoter trap selection.
T9177 16433-16435 PRP denotes We
T9178 16436-16444 VBD denotes replaced
T9179 16445-16448 DT denotes all
T9180 16449-16451 IN denotes of
T9181 16452-16455 DT denotes the
T9182 16461-16466 NNS denotes exons
T9183 16456-16460 NN denotes ESG1
T9184 16467-16471 IN denotes with
T9185 16472-16475 CD denotes two
T9186 16486-16493 NNS denotes vectors
T9187 16476-16485 NN denotes targeting
T9188 16494-16504 VBG denotes containing
T9189 16505-16511 CC denotes either
T9190 16526-16534 NN denotes cassette
T9191 16512-16514 DT denotes an
T9192 16515-16519 NN denotes IRES
T9193 16522-16525 NN denotes geo
T9194 16519-16520 HYPH denotes -
T9195 16520-16521 NN denotes β
T9196 16521-16522 HYPH denotes -
T9197 16535-16536 -LRB- denotes [
T9198 16536-16538 CD denotes 21
T9199 16538-16539 -RRB- denotes ]
T9200 16540-16542 CC denotes or
T9201 16543-16545 DT denotes an
T9202 16551-16555 NN denotes HygR
T9203 16546-16550 NN denotes IRES
T9204 16550-16551 HYPH denotes -
T9205 16556-16564 NN denotes cassette
T9206 16565-16567 IN denotes by
T9207 16568-16576 NN denotes promoter
T9208 16577-16581 NN denotes trap
T9209 16582-16591 NN denotes selection
T9210 16591-16592 . denotes .
T9211 16592-16784 sentence denotes We amplified the 5' arm (1.8 kbp) using KOD plus (TOYOBO) with the pH34-targetpair5-U (5'-CCGCGGAAAGTCAAGAGATTGGGTGG-3') and pH34-targetpair5-L (5'-GCGGCCGCCTTTACGGGTCACGAGGGTCAC-3') primers.
T9212 16593-16595 PRP denotes We
T9213 16596-16605 VBD denotes amplified
T9214 16606-16609 DT denotes the
T9215 16613-16616 NN denotes arm
T9216 16610-16611 CD denotes 5
T9217 16611-16612 SYM denotes '
T9218 16617-16618 -LRB- denotes (
T9219 16622-16625 NN denotes kbp
T9220 16618-16621 CD denotes 1.8
T9221 16625-16626 -RRB- denotes )
T9222 16627-16632 VBG denotes using
T9223 16633-16636 NN denotes KOD
T9224 16637-16641 NN denotes plus
T9225 16642-16643 -LRB- denotes (
T9226 16643-16649 NN denotes TOYOBO
T9227 16649-16650 -RRB- denotes )
T9228 16651-16655 IN denotes with
T9229 16656-16659 DT denotes the
T9230 16776-16783 NNS denotes primers
T9231 16660-16664 NN denotes pH34
T9232 16677-16678 NN denotes U
T9233 16664-16665 HYPH denotes -
T9234 16665-16676 NN denotes targetpair5
T9235 16676-16677 HYPH denotes -
T9236 16679-16680 -LRB- denotes (
T9237 16683-16709 NN denotes CCGCGGAAAGTCAAGAGATTGGGTGG
T9238 16680-16681 CD denotes 5
T9239 16681-16682 SYM denotes '
T9240 16682-16683 HYPH denotes -
T9241 16709-16710 HYPH denotes -
T9242 16710-16711 CD denotes 3
T9243 16711-16712 SYM denotes '
T9244 16712-16713 -RRB- denotes )
T9245 16714-16717 CC denotes and
T9246 16718-16722 NN denotes pH34
T9247 16735-16736 NN denotes L
T9248 16722-16723 HYPH denotes -
T9249 16723-16734 NN denotes targetpair5
T9250 16734-16735 HYPH denotes -
T9251 16737-16738 -LRB- denotes (
T9252 16741-16771 NN denotes GCGGCCGCCTTTACGGGTCACGAGGGTCAC
T9253 16738-16739 CD denotes 5
T9254 16739-16740 SYM denotes '
T9255 16740-16741 HYPH denotes -
T9256 16771-16772 HYPH denotes -
T9257 16772-16773 CD denotes 3
T9258 16773-16774 SYM denotes '
T9259 16774-16775 -RRB- denotes )
T9260 16783-16784 . denotes .
T9261 16784-16950 sentence denotes The 3' arm (5.8 kbp) was amplified using the pH34-targetpair3-U (5'-TGTGGCCAGTGTTTGGTTCTGGCGGG-3') and pH34-targetpair3-L (5'-CTCGAGGACTCGCCATTCTAGCCAAG-3') primers.
T9262 16785-16788 DT denotes The
T9263 16792-16795 NN denotes arm
T9264 16789-16790 CD denotes 3
T9265 16790-16791 SYM denotes '
T9266 16810-16819 VBN denotes amplified
T9267 16796-16797 -LRB- denotes (
T9268 16801-16804 NN denotes kbp
T9269 16797-16800 CD denotes 5.8
T9270 16804-16805 -RRB- denotes )
T9271 16806-16809 VBD denotes was
T9272 16820-16825 VBG denotes using
T9273 16826-16829 DT denotes the
T9274 16942-16949 NNS denotes primers
T9275 16830-16834 NN denotes pH34
T9276 16847-16848 NN denotes U
T9277 16834-16835 HYPH denotes -
T9278 16835-16846 NN denotes targetpair3
T9279 16846-16847 HYPH denotes -
T9280 16849-16850 -LRB- denotes (
T9281 16853-16879 NN denotes TGTGGCCAGTGTTTGGTTCTGGCGGG
T9282 16850-16851 CD denotes 5
T9283 16851-16852 SYM denotes '
T9284 16852-16853 HYPH denotes -
T9285 16879-16880 HYPH denotes -
T9286 16880-16881 CD denotes 3
T9287 16881-16882 SYM denotes '
T9288 16882-16883 -RRB- denotes )
T9289 16884-16887 CC denotes and
T9290 16888-16892 NN denotes pH34
T9291 16905-16906 NN denotes L
T9292 16892-16893 HYPH denotes -
T9293 16893-16904 NN denotes targetpair3
T9294 16904-16905 HYPH denotes -
T9295 16907-16908 -LRB- denotes (
T9296 16911-16937 NN denotes CTCGAGGACTCGCCATTCTAGCCAAG
T9297 16908-16909 CD denotes 5
T9298 16909-16910 SYM denotes '
T9299 16910-16911 HYPH denotes -
T9300 16937-16938 HYPH denotes -
T9301 16938-16939 CD denotes 3
T9302 16939-16940 SYM denotes '
T9303 16940-16941 -RRB- denotes )
T9304 16949-16950 . denotes .
T9305 16950-17035 sentence denotes The IRES β-geo or IRES HygR cassettes were ligated in between the two PCR fragments.
T9306 16951-16954 DT denotes The
T9307 16979-16988 NNS denotes cassettes
T9308 16955-16959 NN denotes IRES
T9309 16962-16965 NN denotes geo
T9310 16960-16961 NN denotes β
T9311 16961-16962 HYPH denotes -
T9312 16966-16968 CC denotes or
T9313 16969-16973 NN denotes IRES
T9314 16974-16978 NN denotes HygR
T9315 16994-17001 VBN denotes ligated
T9316 16989-16993 VBD denotes were
T9317 17002-17004 IN denotes in
T9318 17005-17012 IN denotes between
T9319 17013-17016 DT denotes the
T9320 17025-17034 NNS denotes fragments
T9321 17017-17020 CD denotes two
T9322 17021-17024 NN denotes PCR
T9323 17034-17035 . denotes .
T9324 17035-17104 sentence denotes The diphtheria toxin A cassette was placed downstream of the 3' arm.
T9325 17036-17039 DT denotes The
T9326 17059-17067 NN denotes cassette
T9327 17040-17050 NN denotes diphtheria
T9328 17057-17058 NN denotes A
T9329 17051-17056 NN denotes toxin
T9330 17072-17078 VBN denotes placed
T9331 17068-17071 VBD denotes was
T9332 17079-17089 RB denotes downstream
T9333 17090-17092 IN denotes of
T9334 17093-17096 DT denotes the
T9335 17100-17103 NN denotes arm
T9336 17097-17098 CD denotes 3
T9337 17098-17099 SYM denotes '
T9338 17103-17104 . denotes .
T9339 17104-17243 sentence denotes After linearization with SacII, these targeting vectors were electroporated into 2.0 × 107 RF8 ES cells [22] using a Gene pulser (BIORAD).
T9340 17105-17110 IN denotes After
T9341 17166-17180 VBN denotes electroporated
T9342 17111-17124 NN denotes linearization
T9343 17125-17129 IN denotes with
T9344 17130-17135 NN denotes SacII
T9345 17135-17137 , denotes ,
T9346 17137-17142 DT denotes these
T9347 17153-17160 NNS denotes vectors
T9348 17143-17152 NN denotes targeting
T9349 17161-17165 VBD denotes were
T9350 17181-17185 IN denotes into
T9351 17186-17189 CD denotes 2.0
T9352 17192-17195 CD denotes 107
T9353 17190-17191 SYM denotes ×
T9354 17203-17208 NNS denotes cells
T9355 17196-17199 NN denotes RF8
T9356 17200-17202 NN denotes ES
T9357 17209-17210 -LRB- denotes [
T9358 17210-17212 CD denotes 22
T9359 17212-17213 -RRB- denotes ]
T9360 17214-17219 VBG denotes using
T9361 17220-17221 DT denotes a
T9362 17227-17233 NN denotes pulser
T9363 17222-17226 NN denotes Gene
T9364 17234-17235 -LRB- denotes (
T9365 17235-17241 NNP denotes BIORAD
T9366 17241-17242 -RRB- denotes )
T9367 17242-17243 . denotes .
T9368 17243-17336 sentence denotes Transfected cells were selected with 250 μg/mL G418 or 100 μg/mL hygromycin B, respectively.
T9369 17244-17255 VBN denotes Transfected
T9370 17256-17261 NNS denotes cells
T9371 17267-17275 VBN denotes selected
T9372 17262-17266 VBD denotes were
T9373 17276-17280 IN denotes with
T9374 17281-17284 CD denotes 250
T9375 17285-17287 NN denotes μg
T9376 17291-17295 NN denotes G418
T9377 17287-17288 SYM denotes /
T9378 17288-17290 NN denotes mL
T9379 17296-17298 CC denotes or
T9380 17299-17302 CD denotes 100
T9381 17303-17305 NN denotes μg
T9382 17320-17321 NN denotes B
T9383 17305-17306 SYM denotes /
T9384 17306-17308 NN denotes mL
T9385 17309-17319 NN denotes hygromycin
T9386 17321-17323 , denotes ,
T9387 17323-17335 RB denotes respectively
T9388 17335-17336 . denotes .
T9389 17336-17458 sentence denotes Genomic DNA from G418- or hygromycin B-resistant colonies was screened for homologous recombination by Southern blotting.
T9390 17337-17344 JJ denotes Genomic
T9391 17345-17348 NN denotes DNA
T9392 17399-17407 VBN denotes screened
T9393 17349-17353 IN denotes from
T9394 17354-17358 NN denotes G418
T9395 17376-17385 JJ denotes resistant
T9396 17358-17359 HYPH denotes -
T9397 17360-17362 CC denotes or
T9398 17363-17373 NN denotes hygromycin
T9399 17374-17375 NN denotes B
T9400 17375-17376 HYPH denotes -
T9401 17386-17394 NNS denotes colonies
T9402 17395-17398 VBD denotes was
T9403 17408-17411 IN denotes for
T9404 17412-17422 JJ denotes homologous
T9405 17423-17436 NN denotes recombination
T9406 17437-17439 IN denotes by
T9407 17440-17448 NNP denotes Southern
T9408 17449-17457 NN denotes blotting
T9409 17457-17458 . denotes .
T9832 17460-17468 NNP denotes Southern
T9833 17469-17473 NN denotes blot
T9834 17474-17483 NN denotes screening
T9835 17484-17487 IN denotes for
T9836 17488-17498 JJ denotes homologous
T9837 17499-17512 NN denotes recombination
T9838 17512-17600 sentence denotes ES cells genomic DNA was extracted with PUREGENE™ Cell Lysis Solution (Gentra systems).
T9839 17513-17515 NN denotes ES
T9840 17516-17521 NNS denotes cells
T9841 17530-17533 NN denotes DNA
T9842 17522-17529 JJ denotes genomic
T9843 17538-17547 VBN denotes extracted
T9844 17534-17537 VBD denotes was
T9845 17548-17552 IN denotes with
T9846 17553-17561 NNP denotes PUREGENE
T9847 17574-17582 NN denotes Solution
T9848 17561-17562 SYM denotes ™
T9849 17563-17567 NN denotes Cell
T9850 17568-17573 NN denotes Lysis
T9851 17583-17584 -LRB- denotes (
T9852 17591-17598 NNPS denotes systems
T9853 17584-17590 NNP denotes Gentra
T9854 17598-17599 -RRB- denotes )
T9855 17599-17600 . denotes .
T9856 17600-17766 sentence denotes For 5' Southern blot analysis, genomic DNA was first digested with PstI, then separated on an 0.8% agarose gel and transferred to a nylon membrane as described [20].
T9857 17601-17604 IN denotes For
T9858 17654-17662 VBN denotes digested
T9859 17605-17606 CD denotes 5
T9860 17622-17630 NN denotes analysis
T9861 17606-17607 SYM denotes '
T9862 17608-17616 NNP denotes Southern
T9863 17617-17621 NN denotes blot
T9864 17630-17632 , denotes ,
T9865 17632-17639 JJ denotes genomic
T9866 17640-17643 NN denotes DNA
T9867 17644-17647 VBD denotes was
T9868 17648-17653 RB denotes first
T9869 17663-17667 IN denotes with
T9870 17668-17672 NN denotes PstI
T9871 17672-17674 , denotes ,
T9872 17674-17678 RB denotes then
T9873 17679-17688 VBN denotes separated
T9874 17689-17691 IN denotes on
T9875 17692-17694 DT denotes an
T9876 17708-17711 NN denotes gel
T9877 17695-17698 CD denotes 0.8
T9878 17698-17699 NN denotes %
T9879 17700-17707 NN denotes agarose
T9880 17712-17715 CC denotes and
T9881 17716-17727 VBN denotes transferred
T9882 17728-17730 IN denotes to
T9883 17731-17732 DT denotes a
T9884 17739-17747 NN denotes membrane
T9885 17733-17738 NN denotes nylon
T9886 17748-17750 IN denotes as
T9887 17751-17760 VBN denotes described
T9888 17761-17762 -LRB- denotes [
T9889 17762-17764 CD denotes 20
T9890 17764-17765 -RRB- denotes ]
T9891 17765-17766 . denotes .
T9892 17766-17903 sentence denotes A 560 bp 5' probe was amplified using the ESG1S5 (5'- GATGGTGGTGGTGACTCAGAG -3') and ESG1AS5 as (5'- CCTCCATTGCCTCTATATCAG -3') primers.
T9893 17767-17768 DT denotes A
T9894 17779-17784 NN denotes probe
T9895 17769-17772 CD denotes 560
T9896 17773-17775 NN denotes bp
T9897 17776-17777 CD denotes 5
T9898 17777-17778 SYM denotes '
T9899 17789-17798 VBN denotes amplified
T9900 17785-17788 VBD denotes was
T9901 17799-17804 VBG denotes using
T9902 17805-17808 DT denotes the
T9903 17809-17815 NN denotes ESG1S5
T9904 17816-17817 -LRB- denotes (
T9905 17821-17842 NN denotes GATGGTGGTGGTGACTCAGAG
T9906 17817-17818 CD denotes 5
T9907 17818-17819 SYM denotes '
T9908 17819-17820 HYPH denotes -
T9909 17843-17844 HYPH denotes -
T9910 17844-17845 CD denotes 3
T9911 17845-17846 SYM denotes '
T9912 17846-17847 -RRB- denotes )
T9913 17848-17851 CC denotes and
T9914 17852-17859 NN denotes ESG1AS5
T9915 17860-17862 IN denotes as
T9916 17863-17864 -LRB- denotes (
T9917 17895-17902 NNS denotes primers
T9918 17864-17865 CD denotes 5
T9919 17868-17889 NN denotes CCTCCATTGCCTCTATATCAG
T9920 17865-17866 SYM denotes '
T9921 17866-17867 HYPH denotes -
T9922 17890-17891 HYPH denotes -
T9923 17891-17892 CD denotes 3
T9924 17892-17893 SYM denotes '
T9925 17893-17894 -RRB- denotes )
T9926 17902-17903 . denotes .
T9927 17903-18050 sentence denotes The probe specifically labeled an 18 kbp band from the wild-type locus, a 15 kbp band from the β-geo locus, and a 12 kbp band from the HygR locus.
T9928 17904-17907 DT denotes The
T9929 17908-17913 NN denotes probe
T9930 17927-17934 VBD denotes labeled
T9931 17914-17926 RB denotes specifically
T9932 17935-17937 DT denotes an
T9933 17945-17949 NN denotes band
T9934 17938-17940 CD denotes 18
T9935 17941-17944 NN denotes kbp
T9936 17950-17954 IN denotes from
T9937 17955-17958 DT denotes the
T9938 17969-17974 NN denotes locus
T9939 17959-17963 JJ denotes wild
T9940 17963-17964 HYPH denotes -
T9941 17964-17968 NN denotes type
T9942 17974-17976 , denotes ,
T9943 17976-17977 DT denotes a
T9944 17985-17989 NN denotes band
T9945 17978-17980 CD denotes 15
T9946 17981-17984 NN denotes kbp
T9947 17990-17994 IN denotes from
T9948 17995-17998 DT denotes the
T9949 18005-18010 NN denotes locus
T9950 17999-18000 NN denotes β
T9951 18001-18004 NN denotes geo
T9952 18000-18001 HYPH denotes -
T9953 18010-18012 , denotes ,
T9954 18012-18015 CC denotes and
T9955 18016-18017 DT denotes a
T9956 18025-18029 NN denotes band
T9957 18018-18020 CD denotes 12
T9958 18021-18024 NN denotes kbp
T9959 18030-18034 IN denotes from
T9960 18035-18038 DT denotes the
T9961 18044-18049 NN denotes locus
T9962 18039-18043 NN denotes HygR
T9963 18049-18050 . denotes .
T9964 18050-18121 sentence denotes Genomic DNA was also digested with SpeI for 3' Southern blot analysis.
T9965 18051-18058 JJ denotes Genomic
T9966 18059-18062 NN denotes DNA
T9967 18072-18080 VBN denotes digested
T9968 18063-18066 VBD denotes was
T9969 18067-18071 RB denotes also
T9970 18081-18085 IN denotes with
T9971 18086-18090 NN denotes SpeI
T9972 18091-18094 IN denotes for
T9973 18095-18096 CD denotes 3
T9974 18112-18120 NN denotes analysis
T9975 18096-18097 SYM denotes '
T9976 18098-18106 NNP denotes Southern
T9977 18107-18111 NN denotes blot
T9978 18120-18121 . denotes .
T9979 18121-18268 sentence denotes A 1,010 bp 3' probe was amplified with the pH34U-8000 (5'- CCAACCAGCCAGAGTTTCAGTTAT -3') and pH34L-9000 (5'-GATAAGCTGCTGCCAAAAGACAAG -3') primers.
T9980 18122-18123 DT denotes A
T9981 18136-18141 NN denotes probe
T9982 18124-18129 CD denotes 1,010
T9983 18130-18132 NN denotes bp
T9984 18133-18134 CD denotes 3
T9985 18134-18135 SYM denotes '
T9986 18146-18155 VBN denotes amplified
T9987 18142-18145 VBD denotes was
T9988 18156-18160 IN denotes with
T9989 18161-18164 DT denotes the
T9990 18260-18267 NNS denotes primers
T9991 18165-18170 NN denotes pH34U
T9992 18170-18171 HYPH denotes -
T9993 18171-18175 CD denotes 8000
T9994 18176-18177 -LRB- denotes (
T9995 18181-18205 NN denotes CCAACCAGCCAGAGTTTCAGTTAT
T9996 18177-18178 CD denotes 5
T9997 18178-18179 SYM denotes '
T9998 18179-18180 HYPH denotes -
T9999 18206-18207 HYPH denotes -
T10000 18207-18208 CD denotes 3
T10001 18208-18209 SYM denotes '
T10002 18209-18210 -RRB- denotes )
T10003 18211-18214 CC denotes and
T10004 18215-18220 NN denotes pH34L
T10005 18220-18221 HYPH denotes -
T10006 18221-18225 CD denotes 9000
T10007 18226-18227 -LRB- denotes (
T10008 18230-18254 NN denotes GATAAGCTGCTGCCAAAAGACAAG
T10009 18227-18228 CD denotes 5
T10010 18228-18229 SYM denotes '
T10011 18229-18230 HYPH denotes -
T10012 18255-18256 HYPH denotes -
T10013 18256-18257 CD denotes 3
T10014 18257-18258 SYM denotes '
T10015 18258-18259 -RRB- denotes )
T10016 18267-18268 . denotes .
T10017 18268-18413 sentence denotes The probe hybridized to an 11.5 kbp band from the wild-type locus, a 12.5 kbp band from the β-geo locus, and a 9.5 kbp band from the HygR locus.
T10018 18269-18272 DT denotes The
T10019 18273-18278 NN denotes probe
T10020 18279-18289 VBD denotes hybridized
T10021 18290-18292 IN denotes to
T10022 18293-18295 DT denotes an
T10023 18305-18309 NN denotes band
T10024 18296-18300 CD denotes 11.5
T10025 18301-18304 NN denotes kbp
T10026 18310-18314 IN denotes from
T10027 18315-18318 DT denotes the
T10028 18329-18334 NN denotes locus
T10029 18319-18323 JJ denotes wild
T10030 18324-18328 NN denotes type
T10031 18323-18324 HYPH denotes -
T10032 18334-18336 , denotes ,
T10033 18336-18337 DT denotes a
T10034 18347-18351 NN denotes band
T10035 18338-18342 CD denotes 12.5
T10036 18343-18346 NN denotes kbp
T10037 18352-18356 IN denotes from
T10038 18357-18360 DT denotes the
T10039 18367-18372 NN denotes locus
T10040 18361-18362 NN denotes β
T10041 18363-18366 NN denotes geo
T10042 18362-18363 HYPH denotes -
T10043 18372-18374 , denotes ,
T10044 18374-18377 CC denotes and
T10045 18378-18379 DT denotes a
T10046 18388-18392 NN denotes band
T10047 18380-18383 CD denotes 9.5
T10048 18384-18387 NN denotes kbp
T10049 18393-18397 IN denotes from
T10050 18398-18401 DT denotes the
T10051 18407-18412 NN denotes locus
T10052 18402-18406 NN denotes HygR
T10053 18412-18413 . denotes .
T10414 18415-18425 NN denotes Generation
T10415 18426-18428 IN denotes of
T10416 18429-18438 JJ denotes anti-ESG1
T10417 18450-18460 NNS denotes antibodies
T10418 18439-18449 JJ denotes polyclonal
T10419 18460-18637 sentence denotes The coding sequence of Esg1 was amplified by PCR with the pH34-gw-s (5'- AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA-3') and pH34-gw-as (5'- AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA-3') primers.
T10420 18461-18464 DT denotes The
T10421 18472-18480 NN denotes sequence
T10422 18465-18471 VBG denotes coding
T10423 18493-18502 VBN denotes amplified
T10424 18481-18483 IN denotes of
T10425 18484-18488 NN denotes Esg1
T10426 18489-18492 VBD denotes was
T10427 18503-18505 IN denotes by
T10428 18506-18509 NN denotes PCR
T10429 18510-18514 IN denotes with
T10430 18515-18518 DT denotes the
T10431 18629-18636 NNS denotes primers
T10432 18519-18523 NN denotes pH34
T10433 18527-18528 NN denotes s
T10434 18523-18524 HYPH denotes -
T10435 18524-18526 NN denotes gw
T10436 18526-18527 HYPH denotes -
T10437 18529-18530 -LRB- denotes (
T10438 18534-18567 NN denotes AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
T10439 18530-18531 CD denotes 5
T10440 18531-18532 SYM denotes '
T10441 18532-18533 HYPH denotes -
T10442 18567-18568 HYPH denotes -
T10443 18568-18569 CD denotes 3
T10444 18569-18570 SYM denotes '
T10445 18570-18571 -RRB- denotes )
T10446 18572-18575 CC denotes and
T10447 18576-18580 NN denotes pH34
T10448 18584-18586 NN denotes as
T10449 18580-18581 HYPH denotes -
T10450 18581-18583 NN denotes gw
T10451 18583-18584 HYPH denotes -
T10452 18587-18588 -LRB- denotes (
T10453 18592-18624 NN denotes AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
T10454 18588-18589 CD denotes 5
T10455 18589-18590 SYM denotes '
T10456 18590-18591 HYPH denotes -
T10457 18624-18625 HYPH denotes -
T10458 18625-18626 CD denotes 3
T10459 18626-18627 SYM denotes '
T10460 18627-18628 -RRB- denotes )
T10461 18636-18637 . denotes .
T10462 18637-18730 sentence denotes To construct pDONR-pH34, the resulting PCR product was subcloned into pDONR201 (Invitrogen).
T10463 18638-18640 TO denotes To
T10464 18641-18650 VB denotes construct
T10465 18693-18702 VBN denotes subcloned
T10466 18651-18656 NN denotes pDONR
T10467 18657-18661 NN denotes pH34
T10468 18656-18657 HYPH denotes -
T10469 18661-18663 , denotes ,
T10470 18663-18666 DT denotes the
T10471 18681-18688 NN denotes product
T10472 18667-18676 VBG denotes resulting
T10473 18677-18680 NN denotes PCR
T10474 18689-18692 VBD denotes was
T10475 18703-18707 IN denotes into
T10476 18708-18716 NN denotes pDONR201
T10477 18717-18718 -LRB- denotes (
T10478 18718-18728 NNP denotes Invitrogen
T10479 18728-18729 -RRB- denotes )
T10480 18729-18730 . denotes .
T10481 18730-18803 sentence denotes pDONR-pH34 was interacted with pDEST17 (Invitrogen) by LR recombination.
T10482 18731-18736 NN denotes pDONR
T10483 18737-18741 NN denotes pH34
T10484 18736-18737 HYPH denotes -
T10485 18746-18756 VBN denotes interacted
T10486 18742-18745 VBD denotes was
T10487 18757-18761 IN denotes with
T10488 18762-18769 NN denotes pDEST17
T10489 18770-18771 -LRB- denotes (
T10490 18771-18781 NNP denotes Invitrogen
T10491 18781-18782 -RRB- denotes )
T10492 18783-18785 IN denotes by
T10493 18786-18788 NN denotes LR
T10494 18789-18802 NN denotes recombination
T10495 18802-18803 . denotes .
T10496 18803-18989 sentence denotes After introduction of the resulting expression vector pDEST17-pH34 into BL21-AI E. coli (Invitrogen), recombinant protein production was induced according to the manufacture's protocol.
T10497 18804-18809 IN denotes After
T10498 18941-18948 VBN denotes induced
T10499 18810-18822 NN denotes introduction
T10500 18823-18825 IN denotes of
T10501 18826-18829 DT denotes the
T10502 18851-18857 NN denotes vector
T10503 18830-18839 VBG denotes resulting
T10504 18840-18850 NN denotes expression
T10505 18858-18865 NN denotes pDEST17
T10506 18866-18870 NN denotes pH34
T10507 18865-18866 HYPH denotes -
T10508 18871-18875 IN denotes into
T10509 18876-18880 NN denotes BL21
T10510 18881-18883 NN denotes AI
T10511 18880-18881 HYPH denotes -
T10512 18884-18886 FW denotes E.
T10513 18887-18891 FW denotes coli
T10514 18892-18893 -LRB- denotes (
T10515 18893-18903 NNP denotes Invitrogen
T10516 18903-18904 -RRB- denotes )
T10517 18904-18906 , denotes ,
T10518 18906-18917 JJ denotes recombinant
T10519 18918-18925 NN denotes protein
T10520 18926-18936 NN denotes production
T10521 18937-18940 VBD denotes was
T10522 18949-18958 VBG denotes according
T10523 18959-18961 IN denotes to
T10524 18962-18965 DT denotes the
T10525 18966-18977 NN denotes manufacture
T10526 18980-18988 NN denotes protocol
T10527 18977-18979 POS denotes 's
T10528 18988-18989 . denotes .
T10529 18989-19129 sentence denotes Histidine-tagged ESG1 was purified using Ni-nitrilotriacetic acid agarose (Qiagen) under denaturing conditions in the presence of 8 M urea.
T10530 18990-18999 NN denotes Histidine
T10531 19000-19006 VBN denotes tagged
T10532 18999-19000 HYPH denotes -
T10533 19007-19011 NN denotes ESG1
T10534 19016-19024 VBN denotes purified
T10535 19012-19015 VBD denotes was
T10536 19025-19030 VBG denotes using
T10537 19031-19033 NN denotes Ni
T10538 19056-19063 NN denotes agarose
T10539 19033-19034 : denotes -
T10540 19034-19050 JJ denotes nitrilotriacetic
T10541 19051-19055 NN denotes acid
T10542 19064-19065 -LRB- denotes (
T10543 19065-19071 NNP denotes Qiagen
T10544 19071-19072 -RRB- denotes )
T10545 19073-19078 IN denotes under
T10546 19079-19089 NN denotes denaturing
T10547 19090-19100 NNS denotes conditions
T10548 19101-19103 IN denotes in
T10549 19104-19107 DT denotes the
T10550 19108-19116 NN denotes presence
T10551 19117-19119 IN denotes of
T10552 19120-19121 CD denotes 8
T10553 19122-19123 NN denotes M
T10554 19124-19128 NN denotes urea
T10555 19128-19129 . denotes .
T10556 19129-19277 sentence denotes After dialysis against 6 M urea, the recombinant proteins were injected into New Zealand White rabbits to generate anti-ESG1 polyclonal antibodies.
T10557 19130-19135 IN denotes After
T10558 19193-19201 VBN denotes injected
T10559 19136-19144 NN denotes dialysis
T10560 19145-19152 IN denotes against
T10561 19153-19154 CD denotes 6
T10562 19155-19156 NN denotes M
T10563 19157-19161 NN denotes urea
T10564 19161-19163 , denotes ,
T10565 19163-19166 DT denotes the
T10566 19179-19187 NN denotes proteins
T10567 19167-19178 JJ denotes recombinant
T10568 19188-19192 VBD denotes were
T10569 19202-19206 IN denotes into
T10570 19207-19210 NNP denotes New
T10571 19211-19218 NNP denotes Zealand
T10572 19225-19232 NNS denotes rabbits
T10573 19219-19224 JJ denotes White
T10574 19233-19235 TO denotes to
T10575 19236-19244 VB denotes generate
T10576 19245-19254 JJ denotes anti-ESG1
T10577 19266-19276 NNS denotes antibodies
T10578 19255-19265 JJ denotes polyclonal
T10579 19276-19277 . denotes .
T10856 19279-19286 NNP denotes Western
T10857 19287-19291 NN denotes blot
T10858 19291-19496 sentence denotes After preparation of ES cell extracts with M-Per (Pierce), cellular proteins were separated on sodium dodecyl sulfate (SDS)-14% polyacrylamide gels and transferred to nitrocellulose membranes (Millipore).
T10859 19292-19297 IN denotes After
T10860 19374-19383 VBN denotes separated
T10861 19298-19309 NN denotes preparation
T10862 19310-19312 IN denotes of
T10863 19313-19315 NN denotes ES
T10864 19321-19329 NNS denotes extracts
T10865 19316-19320 NN denotes cell
T10866 19330-19334 IN denotes with
T10867 19335-19336 NN denotes M
T10868 19337-19340 NN denotes Per
T10869 19336-19337 HYPH denotes -
T10870 19341-19342 -LRB- denotes (
T10871 19342-19348 NNP denotes Pierce
T10872 19348-19349 -RRB- denotes )
T10873 19349-19351 , denotes ,
T10874 19351-19359 JJ denotes cellular
T10875 19360-19368 NN denotes proteins
T10876 19369-19373 VBD denotes were
T10877 19384-19386 IN denotes on
T10878 19387-19393 NN denotes sodium
T10879 19402-19409 NN denotes sulfate
T10880 19394-19401 NN denotes dodecyl
T10881 19435-19439 NNS denotes gels
T10882 19410-19411 -LRB- denotes (
T10883 19411-19414 NN denotes SDS
T10884 19414-19415 -RRB- denotes )
T10885 19415-19416 HYPH denotes -
T10886 19416-19418 CD denotes 14
T10887 19418-19419 NN denotes %
T10888 19420-19434 NN denotes polyacrylamide
T10889 19440-19443 CC denotes and
T10890 19444-19455 VBN denotes transferred
T10891 19456-19458 IN denotes to
T10892 19459-19473 NN denotes nitrocellulose
T10893 19474-19483 NNS denotes membranes
T10894 19484-19485 -LRB- denotes (
T10895 19485-19494 NNP denotes Millipore
T10896 19494-19495 -RRB- denotes )
T10897 19495-19496 . denotes .
T10898 19496-19693 sentence denotes Membranes were incubated with anti-ESG1 (1/500 dilution), anti-Oct3/4 (1/500; Santa Cruz Biotechnology), anti-CDK4 (1/200; Santa Cruz Biotechnology), and anti-GFP (1/1000; MBL) primary antibodies.
T10899 19497-19506 NNS denotes Membranes
T10900 19512-19521 VBN denotes incubated
T10901 19507-19511 VBD denotes were
T10902 19522-19526 IN denotes with
T10903 19527-19536 NN denotes anti-ESG1
T10904 19682-19692 NNS denotes antibodies
T10905 19537-19538 -LRB- denotes (
T10906 19544-19552 NN denotes dilution
T10907 19538-19539 CD denotes 1
T10908 19540-19543 CD denotes 500
T10909 19539-19540 SYM denotes /
T10910 19552-19553 -RRB- denotes )
T10911 19553-19555 , denotes ,
T10912 19555-19564 NN denotes anti-Oct3
T10913 19564-19565 HYPH denotes /
T10914 19565-19566 CD denotes 4
T10915 19567-19568 -LRB- denotes (
T10916 19586-19599 NNP denotes Biotechnology
T10917 19568-19569 CD denotes 1
T10918 19570-19573 CD denotes 500
T10919 19569-19570 SYM denotes /
T10920 19573-19574 : denotes ;
T10921 19575-19580 NNP denotes Santa
T10922 19581-19585 NNP denotes Cruz
T10923 19599-19600 -RRB- denotes )
T10924 19600-19602 , denotes ,
T10925 19602-19611 NN denotes anti-CDK4
T10926 19612-19613 -LRB- denotes (
T10927 19631-19644 NNP denotes Biotechnology
T10928 19613-19614 CD denotes 1
T10929 19615-19618 CD denotes 200
T10930 19614-19615 SYM denotes /
T10931 19618-19619 : denotes ;
T10932 19620-19625 NNP denotes Santa
T10933 19626-19630 NNP denotes Cruz
T10934 19644-19645 -RRB- denotes )
T10935 19645-19647 , denotes ,
T10936 19647-19650 CC denotes and
T10937 19651-19659 NN denotes anti-GFP
T10938 19660-19661 -LRB- denotes (
T10939 19669-19672 NN denotes MBL
T10940 19661-19662 CD denotes 1
T10941 19663-19667 CD denotes 1000
T10942 19662-19663 : denotes /
T10943 19667-19668 : denotes ;
T10944 19672-19673 -RRB- denotes )
T10945 19674-19681 JJ denotes primary
T10946 19692-19693 . denotes .
T10947 19693-19833 sentence denotes Horseradish peroxidase-conjugated anti-rabbit and anti-mouse immunoglobulins (1/5000; Cell Signaling) were used to detect antibody binding.
T10948 19694-19705 NN denotes Horseradish
T10949 19706-19716 NN denotes peroxidase
T10950 19717-19727 VBN denotes conjugated
T10951 19716-19717 HYPH denotes -
T10952 19755-19770 NNS denotes immunoglobulins
T10953 19728-19739 JJ denotes anti-rabbit
T10954 19740-19743 CC denotes and
T10955 19744-19754 JJ denotes anti-mouse
T10956 19801-19805 VBN denotes used
T10957 19771-19772 -LRB- denotes (
T10958 19785-19794 NN denotes Signaling
T10959 19772-19773 CD denotes 1
T10960 19774-19778 CD denotes 5000
T10961 19773-19774 SYM denotes /
T10962 19778-19779 : denotes ;
T10963 19780-19784 NN denotes Cell
T10964 19794-19795 -RRB- denotes )
T10965 19796-19800 VBD denotes were
T10966 19806-19808 TO denotes to
T10967 19809-19815 VB denotes detect
T10968 19816-19824 NN denotes antibody
T10969 19825-19832 NN denotes binding
T10970 19832-19833 . denotes .
T10971 19833-19920 sentence denotes We visualized bound antibody with an ECL Western Blotting Detection System (Amersham).
T10972 19834-19836 PRP denotes We
T10973 19837-19847 VBD denotes visualized
T10974 19848-19853 VBN denotes bound
T10975 19854-19862 NN denotes antibody
T10976 19863-19867 IN denotes with
T10977 19868-19870 DT denotes an
T10978 19902-19908 NNP denotes System
T10979 19871-19874 NNP denotes ECL
T10980 19875-19882 NNP denotes Western
T10981 19883-19891 NNP denotes Blotting
T10982 19892-19901 NNP denotes Detection
T10983 19909-19910 -LRB- denotes (
T10984 19910-19918 NNP denotes Amersham
T10985 19918-19919 -RRB- denotes )
T10986 19919-19920 . denotes .
T11476 19922-19932 NN denotes Derivation
T11477 19933-19935 IN denotes of
T11478 19936-19940 NN denotes ESG1
T11479 19941-19950 JJ denotes deficient
T11480 19940-19941 HYPH denotes -
T11481 19954-19959 NNS denotes cells
T11482 19951-19953 NN denotes ES
T11483 19960-19964 IN denotes from
T11484 19965-19976 NNS denotes blastocysts
T11485 19976-20120 sentence denotes Esg1+/-or ESG1-/- mutant female mice were injected with Tamoxifen (10 μg) and Depo-provera (1 mg) subcutaneously on the third day of pregnancy.
T11486 19977-19981 NN denotes Esg1
T11487 20009-20013 NNS denotes mice
T11488 19981-19982 SYM denotes +
T11489 19982-19983 HYPH denotes /
T11490 19983-19984 SYM denotes -
T11491 19984-19986 CC denotes or
T11492 19987-19991 NN denotes ESG1
T11493 19991-19992 SYM denotes -
T11494 19992-19993 HYPH denotes /
T11495 19993-19994 SYM denotes -
T11496 19995-20001 NN denotes mutant
T11497 20002-20008 JJ denotes female
T11498 20019-20027 VBN denotes injected
T11499 20014-20018 VBD denotes were
T11500 20028-20032 IN denotes with
T11501 20033-20042 NN denotes Tamoxifen
T11502 20043-20044 -LRB- denotes (
T11503 20047-20049 NN denotes μg
T11504 20044-20046 CD denotes 10
T11505 20049-20050 -RRB- denotes )
T11506 20051-20054 CC denotes and
T11507 20055-20059 NN denotes Depo
T11508 20060-20067 NN denotes provera
T11509 20059-20060 HYPH denotes -
T11510 20068-20069 -LRB- denotes (
T11511 20071-20073 NN denotes mg
T11512 20069-20070 CD denotes 1
T11513 20073-20074 -RRB- denotes )
T11514 20075-20089 RB denotes subcutaneously
T11515 20090-20092 IN denotes on
T11516 20093-20096 DT denotes the
T11517 20103-20106 NN denotes day
T11518 20097-20102 JJ denotes third
T11519 20107-20109 IN denotes of
T11520 20110-20119 NN denotes pregnancy
T11521 20119-20120 . denotes .
T11522 20120-20504 sentence denotes Four days later, embryos in diapause were flushed out of the uterus and cultured on STO feeder cells in four-well plates in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 20% Fetal Bovine Serum (Hyclone), 0.1 mM Non-Essential Amino Acids (Invitrogen), 2 mM L-glutamine (Invitrogen), 50 U/ml Penicillin-Streptomysin (Invitrogen), and 0.11 mM 2-mercaptoethanol (Invitrogen).
T11523 20121-20125 CD denotes Four
T11524 20126-20130 NNS denotes days
T11525 20131-20136 RB denotes later
T11526 20163-20170 VBN denotes flushed
T11527 20136-20138 , denotes ,
T11528 20138-20145 NNS denotes embryos
T11529 20146-20148 IN denotes in
T11530 20149-20157 NN denotes diapause
T11531 20158-20162 VBD denotes were
T11532 20171-20174 IN denotes out
T11533 20175-20177 IN denotes of
T11534 20178-20181 DT denotes the
T11535 20182-20188 NN denotes uterus
T11536 20189-20192 CC denotes and
T11537 20193-20201 VBN denotes cultured
T11538 20202-20204 IN denotes on
T11539 20205-20208 NN denotes STO
T11540 20216-20221 NNS denotes cells
T11541 20209-20215 NN denotes feeder
T11542 20222-20224 IN denotes in
T11543 20225-20229 CD denotes four
T11544 20230-20234 NN denotes well
T11545 20229-20230 HYPH denotes -
T11546 20235-20241 NNS denotes plates
T11547 20242-20244 IN denotes in
T11548 20245-20253 NNP denotes Dulbecco
T11549 20271-20277 NNP denotes Medium
T11550 20253-20255 POS denotes 's
T11551 20256-20264 VBN denotes Modified
T11552 20265-20270 NNP denotes Eagle
T11553 20278-20279 -LRB- denotes (
T11554 20279-20283 NN denotes DMEM
T11555 20283-20284 -RRB- denotes )
T11556 20285-20297 VBN denotes supplemented
T11557 20298-20302 IN denotes with
T11558 20303-20305 CD denotes 20
T11559 20305-20306 NN denotes %
T11560 20320-20325 NN denotes Serum
T11561 20307-20312 JJ denotes Fetal
T11562 20313-20319 JJ denotes Bovine
T11563 20326-20327 -LRB- denotes (
T11564 20327-20334 NN denotes Hyclone
T11565 20334-20335 -RRB- denotes )
T11566 20335-20337 , denotes ,
T11567 20337-20340 CD denotes 0.1
T11568 20341-20343 NN denotes mM
T11569 20364-20369 NNS denotes Acids
T11570 20344-20357 JJ denotes Non-Essential
T11571 20358-20363 NN denotes Amino
T11572 20370-20371 -LRB- denotes (
T11573 20371-20381 NNP denotes Invitrogen
T11574 20381-20382 -RRB- denotes )
T11575 20382-20384 , denotes ,
T11576 20384-20385 CD denotes 2
T11577 20386-20388 NN denotes mM
T11578 20391-20400 NN denotes glutamine
T11579 20389-20390 NN denotes L
T11580 20390-20391 HYPH denotes -
T11581 20401-20402 -LRB- denotes (
T11582 20402-20412 NNP denotes Invitrogen
T11583 20412-20413 -RRB- denotes )
T11584 20413-20415 , denotes ,
T11585 20415-20417 CD denotes 50
T11586 20418-20419 NN denotes U
T11587 20434-20446 NN denotes Streptomysin
T11588 20419-20420 SYM denotes /
T11589 20420-20422 NN denotes ml
T11590 20423-20433 NN denotes Penicillin
T11591 20433-20434 HYPH denotes -
T11592 20447-20448 -LRB- denotes (
T11593 20448-20458 NNP denotes Invitrogen
T11594 20458-20459 -RRB- denotes )
T11595 20459-20461 , denotes ,
T11596 20461-20464 CC denotes and
T11597 20465-20469 CD denotes 0.11
T11598 20470-20472 NN denotes mM
T11599 20475-20490 NN denotes mercaptoethanol
T11600 20473-20474 CD denotes 2
T11601 20474-20475 HYPH denotes -
T11602 20491-20492 -LRB- denotes (
T11603 20492-20502 NNP denotes Invitrogen
T11604 20502-20503 -RRB- denotes )
T11605 20503-20504 . denotes .
T11606 20504-20634 sentence denotes After six days, the central mass of each explant was harvested, rinsed in PBS, and placed in a drop of trypsin for a few minutes.
T11607 20505-20510 IN denotes After
T11608 20558-20567 VBN denotes harvested
T11609 20511-20514 CD denotes six
T11610 20515-20519 NNS denotes days
T11611 20519-20521 , denotes ,
T11612 20521-20524 DT denotes the
T11613 20533-20537 NN denotes mass
T11614 20525-20532 JJ denotes central
T11615 20538-20540 IN denotes of
T11616 20541-20545 DT denotes each
T11617 20546-20553 NN denotes explant
T11618 20554-20557 VBD denotes was
T11619 20567-20569 , denotes ,
T11620 20569-20575 VBN denotes rinsed
T11621 20576-20578 IN denotes in
T11622 20579-20582 NN denotes PBS
T11623 20582-20584 , denotes ,
T11624 20584-20587 CC denotes and
T11625 20588-20594 VBN denotes placed
T11626 20595-20597 IN denotes in
T11627 20598-20599 DT denotes a
T11628 20600-20604 NN denotes drop
T11629 20605-20607 IN denotes of
T11630 20608-20615 NN denotes trypsin
T11631 20616-20619 IN denotes for
T11632 20620-20621 DT denotes a
T11633 20626-20633 NNS denotes minutes
T11634 20622-20625 JJ denotes few
T11635 20633-20634 . denotes .
T11636 20634-20768 sentence denotes The cell mass was collected with a finely drawn-out Pasteur pipette preloaded with medium, ensuring minimal carryover of the trypsin.
T11637 20635-20638 DT denotes The
T11638 20644-20648 NN denotes mass
T11639 20639-20643 NN denotes cell
T11640 20653-20662 VBN denotes collected
T11641 20649-20652 VBD denotes was
T11642 20663-20667 IN denotes with
T11643 20668-20669 DT denotes a
T11644 20695-20702 NN denotes pipette
T11645 20670-20676 RB denotes finely
T11646 20677-20682 VBN denotes drawn
T11647 20682-20683 HYPH denotes -
T11648 20683-20686 RP denotes out
T11649 20687-20694 NNP denotes Pasteur
T11650 20703-20712 VBN denotes preloaded
T11651 20713-20717 IN denotes with
T11652 20718-20724 NN denotes medium
T11653 20724-20726 , denotes ,
T11654 20726-20734 VBG denotes ensuring
T11655 20735-20742 JJ denotes minimal
T11656 20743-20752 NN denotes carryover
T11657 20753-20755 IN denotes of
T11658 20756-20759 DT denotes the
T11659 20760-20767 NN denotes trypsin
T11660 20767-20768 . denotes .
T11661 20768-20851 sentence denotes The cells were gently transfered into a fresh well with 20% FBS-containing medium.
T11662 20769-20772 DT denotes The
T11663 20773-20778 NNS denotes cells
T11664 20791-20801 VBN denotes transfered
T11665 20779-20783 VBD denotes were
T11666 20784-20790 RB denotes gently
T11667 20802-20806 IN denotes into
T11668 20807-20808 DT denotes a
T11669 20815-20819 NN denotes well
T11670 20809-20814 JJ denotes fresh
T11671 20820-20824 IN denotes with
T11672 20825-20827 CD denotes 20
T11673 20827-20828 NN denotes %
T11674 20844-20850 NN denotes medium
T11675 20829-20832 NN denotes FBS
T11676 20833-20843 VBG denotes containing
T11677 20832-20833 HYPH denotes -
T11678 20850-20851 . denotes .
T11679 20851-20983 sentence denotes The resulting primary ES cell colonies were individually passaged into wells of four-well plates containing STO feeder cell layers.
T11680 20852-20855 DT denotes The
T11681 20882-20890 NNS denotes colonies
T11682 20856-20865 VBG denotes resulting
T11683 20866-20873 JJ denotes primary
T11684 20874-20876 NN denotes ES
T11685 20877-20881 NN denotes cell
T11686 20909-20917 VBN denotes passaged
T11687 20891-20895 VBD denotes were
T11688 20896-20908 RB denotes individually
T11689 20918-20922 IN denotes into
T11690 20923-20928 NNS denotes wells
T11691 20929-20931 IN denotes of
T11692 20932-20936 CD denotes four
T11693 20937-20941 NN denotes well
T11694 20936-20937 HYPH denotes -
T11695 20942-20948 NNS denotes plates
T11696 20949-20959 VBG denotes containing
T11697 20960-20963 NN denotes STO
T11698 20976-20982 NNS denotes layers
T11699 20964-20970 NN denotes feeder
T11700 20971-20975 NN denotes cell
T11701 20982-20983 . denotes .
T11702 20983-21056 sentence denotes Thereafter, cells were expanded by trypsinization of the entire culture.
T11703 20984-20994 RB denotes Thereafter
T11704 21007-21015 VBN denotes expanded
T11705 20994-20996 , denotes ,
T11706 20996-21001 NNS denotes cells
T11707 21002-21006 VBD denotes were
T11708 21016-21018 IN denotes by
T11709 21019-21033 NN denotes trypsinization
T11710 21034-21036 IN denotes of
T11711 21037-21040 DT denotes the
T11712 21048-21055 NN denotes culture
T11713 21041-21047 JJ denotes entire
T11714 21055-21056 . denotes .
T11883 21058-21069 NNS denotes Microarrays
T11884 21069-21168 sentence denotes Total RNA from wild-type ES cells and ESG1-/- ES cells was labeled with Cy3 and Cy5, respectively.
T11885 21070-21075 JJ denotes Total
T11886 21076-21079 NN denotes RNA
T11887 21129-21136 VBN denotes labeled
T11888 21080-21084 IN denotes from
T11889 21085-21089 JJ denotes wild
T11890 21090-21094 NN denotes type
T11891 21089-21090 HYPH denotes -
T11892 21098-21103 NNS denotes cells
T11893 21095-21097 NN denotes ES
T11894 21104-21107 CC denotes and
T11895 21108-21112 NN denotes ESG1
T11896 21119-21124 NNS denotes cells
T11897 21112-21113 SYM denotes -
T11898 21113-21114 HYPH denotes /
T11899 21114-21115 SYM denotes -
T11900 21116-21118 NN denotes ES
T11901 21125-21128 VBD denotes was
T11902 21137-21141 IN denotes with
T11903 21142-21145 NN denotes Cy3
T11904 21146-21149 CC denotes and
T11905 21150-21153 NN denotes Cy5
T11906 21153-21155 , denotes ,
T11907 21155-21167 RB denotes respectively
T11908 21167-21168 . denotes .
T11909 21168-21283 sentence denotes The samples were hybridized to a Mouse Development Microarray (Algilent) according to the manufacturer's protocol.
T11910 21169-21172 DT denotes The
T11911 21173-21180 NNS denotes samples
T11912 21186-21196 VBN denotes hybridized
T11913 21181-21185 VBD denotes were
T11914 21197-21199 IN denotes to
T11915 21200-21201 DT denotes a
T11916 21220-21230 NN denotes Microarray
T11917 21202-21207 NN denotes Mouse
T11918 21208-21219 NN denotes Development
T11919 21231-21232 -LRB- denotes (
T11920 21232-21240 NNP denotes Algilent
T11921 21240-21241 -RRB- denotes )
T11922 21242-21251 VBG denotes according
T11923 21252-21254 IN denotes to
T11924 21255-21258 DT denotes the
T11925 21259-21271 NN denotes manufacturer
T11926 21274-21282 NN denotes protocol
T11927 21271-21273 POS denotes 's
T11928 21282-21283 . denotes .
T11929 21283-21355 sentence denotes Arrays were scanned with a G2565BA Microarray Scanner System (Agilent).
T11930 21284-21290 NNS denotes Arrays
T11931 21296-21303 VBN denotes scanned
T11932 21291-21295 VBD denotes were
T11933 21304-21308 IN denotes with
T11934 21309-21310 DT denotes a
T11935 21338-21344 NN denotes System
T11936 21311-21318 NN denotes G2565BA
T11937 21319-21329 NN denotes Microarray
T11938 21330-21337 NN denotes Scanner
T11939 21345-21346 -LRB- denotes (
T11940 21346-21353 NNP denotes Agilent
T11941 21353-21354 -RRB- denotes )
T11942 21354-21355 . denotes .
T11943 21355-21411 sentence denotes Hybridization was repeated with two independent clones.
T11944 21356-21369 NN denotes Hybridization
T11945 21374-21382 VBN denotes repeated
T11946 21370-21373 VBD denotes was
T11947 21383-21387 IN denotes with
T11948 21388-21391 CD denotes two
T11949 21404-21410 NNS denotes clones
T11950 21392-21403 JJ denotes independent
T11951 21410-21411 . denotes .
T11952 21411-21475 sentence denotes Data were analyzed with GeneSprings software (Silico Genetics).
T11953 21412-21416 NNS denotes Data
T11954 21422-21430 VBN denotes analyzed
T11955 21417-21421 VBD denotes were
T11956 21431-21435 IN denotes with
T11957 21436-21447 NNP denotes GeneSprings
T11958 21448-21456 NN denotes software
T11959 21457-21458 -LRB- denotes (
T11960 21465-21473 NNP denotes Genetics
T11961 21458-21464 NNP denotes Silico
T11962 21473-21474 -RRB- denotes )
T11963 21474-21475 . denotes .
T12055 5103-5118 NNS denotes ESG1pseudogenes
T12056 5119-5129 VBN denotes identified
T12057 5130-5132 IN denotes by
T12058 5133-5134 DT denotes a
T12059 5141-5147 NN denotes search
T12060 5135-5140 NNP denotes Blast
T12061 5148-5150 IN denotes of
T12062 5151-5156 NN denotes mouse
T12063 5165-5174 NNS denotes databases
T12064 5157-5164 JJ denotes genomic
T12065 5174-5175 . denotes .
T12066 5175-5228 sentence denotes Substitution mutations are indicated by black lines.
T12067 5176-5188 NN denotes Substitution
T12068 5189-5198 NNS denotes mutations
T12069 5203-5212 VBN denotes indicated
T12070 5199-5202 VBP denotes are
T12071 5213-5215 IN denotes by
T12072 5216-5221 JJ denotes black
T12073 5222-5227 NNS denotes lines
T12074 5227-5228 . denotes .
T12075 5228-5284 sentence denotes Intron-like gap sequences are indicated with triangles.
T12076 5229-5235 NN denotes Intron
T12077 5236-5240 JJ denotes like
T12078 5235-5236 HYPH denotes -
T12079 5245-5254 NNS denotes sequences
T12080 5241-5244 NN denotes gap
T12081 5259-5268 VBN denotes indicated
T12082 5255-5258 VBP denotes are
T12083 5269-5273 IN denotes with
T12084 5274-5283 NNS denotes triangles
T12085 5283-5284 . denotes .
T12086 5284-5334 sentence denotes Chromosomal localizations are shown on the right.
T12087 5285-5296 JJ denotes Chromosomal
T12088 5297-5310 NNS denotes localizations
T12089 5315-5320 VBN denotes shown
T12090 5311-5314 VBP denotes are
T12091 5321-5323 IN denotes on
T12092 5324-5327 DT denotes the
T12093 5328-5333 NN denotes right
T12094 5333-5334 . denotes .
T12275 6780-6783 NN denotes BAC
T12276 6784-6790 NNS denotes clones
T12277 6791-6801 VBG denotes containing
T12278 6802-6805 DT denotes the
T12279 6811-6815 NN denotes gene
T12280 6806-6810 NN denotes ESG1
T12281 6816-6819 CC denotes and
T12282 6820-6821 DT denotes a
T12283 6834-6844 NN denotes pseudogene
T12284 6822-6833 NN denotes duplication
T12285 6845-6846 -LRB- denotes (
T12286 6846-6848 NN denotes PS
T12287 6848-6849 -RRB- denotes )
T12288 6849-6850 . denotes .
T12289 6850-6902 sentence denotes A) Localization of the gene and PS on chromosome 9.
T12290 6851-6852 LS denotes A
T12291 6854-6866 NN denotes Localization
T12292 6852-6853 -RRB- denotes )
T12293 6867-6869 IN denotes of
T12294 6870-6873 DT denotes the
T12295 6874-6878 NN denotes gene
T12296 6879-6882 CC denotes and
T12297 6883-6885 NN denotes PS
T12298 6886-6888 IN denotes on
T12299 6889-6899 NN denotes chromosome
T12300 6900-6901 CD denotes 9
T12301 6901-6902 . denotes .
T12302 6902-7068 sentence denotes B) Sequence comparison of the gene and PS revealed that these are homologous from eight base pairs upstream of the first exon to 675 bp downstream of the third exon.
T12303 6903-6904 LS denotes B
T12304 6945-6953 VBD denotes revealed
T12305 6904-6905 -RRB- denotes )
T12306 6906-6914 NN denotes Sequence
T12307 6915-6925 NN denotes comparison
T12308 6926-6928 IN denotes of
T12309 6929-6932 DT denotes the
T12310 6933-6937 NN denotes gene
T12311 6938-6941 CC denotes and
T12312 6942-6944 NN denotes PS
T12313 6954-6958 IN denotes that
T12314 6965-6968 VBP denotes are
T12315 6959-6964 DT denotes these
T12316 6969-6979 JJ denotes homologous
T12317 6980-6984 IN denotes from
T12318 6985-6990 CD denotes eight
T12319 6996-7001 NNS denotes pairs
T12320 6991-6995 NN denotes base
T12321 7002-7010 RB denotes upstream
T12322 7011-7013 IN denotes of
T12323 7014-7017 DT denotes the
T12324 7024-7028 NN denotes exon
T12325 7018-7023 JJ denotes first
T12326 7029-7031 IN denotes to
T12327 7032-7035 CD denotes 675
T12328 7036-7038 NN denotes bp
T12329 7039-7049 RB denotes downstream
T12330 7050-7052 IN denotes of
T12331 7053-7056 DT denotes the
T12332 7063-7067 NN denotes exon
T12333 7057-7062 JJ denotes third
T12334 7067-7068 . denotes .
T12335 7068-7121 sentence denotes Substitution mutations are indicated by black lines.
T12336 7069-7081 NN denotes Substitution
T12337 7082-7091 NNS denotes mutations
T12338 7096-7105 VBN denotes indicated
T12339 7092-7095 VBP denotes are
T12340 7106-7108 IN denotes by
T12341 7109-7114 JJ denotes black
T12342 7115-7120 NNS denotes lines
T12343 7120-7121 . denotes .
T12344 7121-7169 sentence denotes The insertion is indicated by an open triangle.
T12345 7122-7125 DT denotes The
T12346 7126-7135 NN denotes insertion
T12347 7139-7148 VBN denotes indicated
T12348 7136-7138 VBZ denotes is
T12349 7149-7151 IN denotes by
T12350 7152-7154 DT denotes an
T12351 7160-7168 NN denotes triangle
T12352 7155-7159 JJ denotes open
T12353 7168-7169 . denotes .
T12543 7180-7188 NN denotes Promoter
T12544 7189-7197 NN denotes enhancer
T12545 7188-7189 HYPH denotes /
T12546 7198-7206 NN denotes activity
T12547 7207-7209 IN denotes of
T12548 7210-7213 DT denotes the
T12549 7219-7223 NN denotes gene
T12550 7214-7218 NN denotes ESG1
T12551 7224-7227 CC denotes and
T12552 7228-7238 NN denotes pseudogene
T12553 7238-7239 . denotes .
T12554 7239-7372 sentence denotes DNA fragments of ~6 kbp isolated from the 5' flanking regions of the gene and PS were transferred into luciferase reporter plasmids.
T12555 7240-7243 NN denotes DNA
T12556 7244-7253 NNS denotes fragments
T12557 7326-7337 VBN denotes transferred
T12558 7254-7256 IN denotes of
T12559 7257-7258 SYM denotes ~
T12560 7258-7259 CD denotes 6
T12561 7260-7263 NN denotes kbp
T12562 7264-7272 VBN denotes isolated
T12563 7273-7277 IN denotes from
T12564 7278-7281 DT denotes the
T12565 7294-7301 NNS denotes regions
T12566 7282-7283 CD denotes 5
T12567 7283-7284 SYM denotes '
T12568 7285-7293 NN denotes flanking
T12569 7302-7304 IN denotes of
T12570 7305-7308 DT denotes the
T12571 7309-7313 NN denotes gene
T12572 7314-7317 CC denotes and
T12573 7318-7320 NN denotes PS
T12574 7321-7325 VBD denotes were
T12575 7338-7342 IN denotes into
T12576 7343-7353 NN denotes luciferase
T12577 7354-7362 NN denotes reporter
T12578 7363-7371 NNS denotes plasmids
T12579 7371-7372 . denotes .
T12580 7372-7534 sentence denotes We introduced the reporter genes into undifferentiated ES cells (open columns), retinoic acid-treated ES cells (grey columns), and NIH3T3 cells (closed columns).
T12581 7373-7375 PRP denotes We
T12582 7376-7386 VBD denotes introduced
T12583 7387-7390 DT denotes the
T12584 7400-7405 NNS denotes genes
T12585 7391-7399 NN denotes reporter
T12586 7406-7410 IN denotes into
T12587 7411-7427 JJ denotes undifferentiated
T12588 7431-7436 NNS denotes cells
T12589 7428-7430 NN denotes ES
T12590 7437-7438 -LRB- denotes (
T12591 7443-7450 NNS denotes columns
T12592 7438-7442 JJ denotes open
T12593 7450-7451 -RRB- denotes )
T12594 7451-7453 , denotes ,
T12595 7453-7461 JJ denotes retinoic
T12596 7462-7466 NN denotes acid
T12597 7467-7474 VBN denotes treated
T12598 7466-7467 HYPH denotes -
T12599 7478-7483 NNS denotes cells
T12600 7475-7477 NN denotes ES
T12601 7484-7485 -LRB- denotes (
T12602 7490-7497 NNS denotes columns
T12603 7485-7489 JJ denotes grey
T12604 7497-7498 -RRB- denotes )
T12605 7498-7500 , denotes ,
T12606 7500-7503 CC denotes and
T12607 7504-7510 NN denotes NIH3T3
T12608 7511-7516 NNS denotes cells
T12609 7517-7518 -LRB- denotes (
T12610 7525-7532 NNS denotes columns
T12611 7518-7524 VBN denotes closed
T12612 7532-7533 -RRB- denotes )
T12613 7533-7534 . denotes .
T12614 7534-7620 sentence denotes Data represent the averages and standard deviations of three independent experiments.
T12615 7535-7539 NNS denotes Data
T12616 7540-7549 VBP denotes represent
T12617 7550-7553 DT denotes the
T12618 7554-7562 NNS denotes averages
T12619 7563-7566 CC denotes and
T12620 7567-7575 JJ denotes standard
T12621 7576-7586 NNS denotes deviations
T12622 7587-7589 IN denotes of
T12623 7590-7595 CD denotes three
T12624 7608-7619 NNS denotes experiments
T12625 7596-7607 JJ denotes independent
T12626 7619-7620 . denotes .
T13078 9403-9411 VBN denotes Targeted
T13079 9412-9422 NN denotes disruption
T13080 9423-9425 IN denotes of
T13081 9426-9429 DT denotes the
T13082 9441-9445 NN denotes gene
T13083 9430-9435 NN denotes mouse
T13084 9436-9440 NN denotes ESG1
T13085 9445-9446 . denotes .
T13086 9446-9469 sentence denotes A) Targeting strategy.
T13087 9447-9448 LS denotes A
T13088 9460-9468 NN denotes strategy
T13089 9448-9449 -RRB- denotes )
T13090 9450-9459 NN denotes Targeting
T13091 9468-9469 . denotes .
T13092 9469-9518 sentence denotes Homologous regions are indicated by thick lines.
T13093 9470-9480 JJ denotes Homologous
T13094 9481-9488 NNS denotes regions
T13095 9493-9502 VBN denotes indicated
T13096 9489-9492 VBP denotes are
T13097 9503-9505 IN denotes by
T13098 9506-9511 JJ denotes thick
T13099 9512-9517 NNS denotes lines
T13100 9517-9518 . denotes .
T13101 9518-9617 sentence denotes Recognition sites of PstI (P) and SpeI (S), which were used for Southern blot analyses, are shown.
T13102 9519-9530 NN denotes Recognition
T13103 9531-9536 NNS denotes sites
T13104 9611-9616 VBN denotes shown
T13105 9537-9539 IN denotes of
T13106 9540-9544 NN denotes PstI
T13107 9545-9546 -LRB- denotes (
T13108 9546-9547 NN denotes P
T13109 9547-9548 -RRB- denotes )
T13110 9549-9552 CC denotes and
T13111 9553-9557 NN denotes SpeI
T13112 9558-9559 -LRB- denotes (
T13113 9559-9560 NN denotes S
T13114 9560-9561 -RRB- denotes )
T13115 9561-9563 , denotes ,
T13116 9563-9568 WDT denotes which
T13117 9574-9578 VBN denotes used
T13118 9569-9573 VBD denotes were
T13119 9579-9582 IN denotes for
T13120 9583-9591 NNP denotes Southern
T13121 9592-9596 NN denotes blot
T13122 9597-9605 NNS denotes analyses
T13123 9605-9607 , denotes ,
T13124 9607-9610 VBP denotes are
T13125 9616-9617 . denotes .
T13126 9617-9746 sentence denotes The gene encoding diphtheria toxin A (DTA) was inserted at the 3' end of the targeting vectors to facilitate negative selection.
T13127 9618-9621 DT denotes The
T13128 9653-9654 NN denotes A
T13129 9622-9626 NN denotes gene
T13130 9627-9635 VBG denotes encoding
T13131 9636-9646 NN denotes diphtheria
T13132 9647-9652 NN denotes toxin
T13133 9665-9673 VBN denotes inserted
T13134 9655-9656 -LRB- denotes (
T13135 9656-9659 NN denotes DTA
T13136 9659-9660 -RRB- denotes )
T13137 9661-9664 VBD denotes was
T13138 9674-9676 IN denotes at
T13139 9677-9680 DT denotes the
T13140 9684-9687 NN denotes end
T13141 9681-9682 CD denotes 3
T13142 9682-9683 SYM denotes '
T13143 9688-9690 IN denotes of
T13144 9691-9694 DT denotes the
T13145 9705-9712 NNS denotes vectors
T13146 9695-9704 NN denotes targeting
T13147 9713-9715 TO denotes to
T13148 9716-9726 VB denotes facilitate
T13149 9727-9735 JJ denotes negative
T13150 9736-9745 NN denotes selection
T13151 9745-9746 . denotes .
T13152 9746-9809 sentence denotes B) Southern blot analyses confirming homologous recombination.
T13153 9747-9748 LS denotes B
T13154 9764-9772 NNS denotes analyses
T13155 9748-9749 -RRB- denotes )
T13156 9750-9758 NNP denotes Southern
T13157 9759-9763 NN denotes blot
T13158 9773-9783 VBG denotes confirming
T13159 9784-9794 JJ denotes homologous
T13160 9795-9808 NN denotes recombination
T13161 9808-9809 . denotes .
T13162 9809-9903 sentence denotes WT, wild-type ES cells; β, β-geo +/- ES cells; H, HygR +/- ES cells; -/-, ESG1-null ES cells.
T13163 9810-9812 NN denotes WT
T13164 9812-9814 , denotes ,
T13165 9814-9818 JJ denotes wild
T13166 9819-9823 NN denotes type
T13167 9818-9819 HYPH denotes -
T13168 9827-9832 NNS denotes cells
T13169 9824-9826 NN denotes ES
T13170 9832-9833 : denotes ;
T13171 9834-9835 NN denotes β
T13172 9835-9837 , denotes ,
T13173 9837-9838 NN denotes β
T13174 9839-9842 NN denotes geo
T13175 9838-9839 HYPH denotes -
T13176 9850-9855 NNS denotes cells
T13177 9843-9844 SYM denotes +
T13178 9844-9845 HYPH denotes /
T13179 9845-9846 SYM denotes -
T13180 9847-9849 NN denotes ES
T13181 9855-9856 : denotes ;
T13182 9857-9858 NN denotes H
T13183 9858-9860 , denotes ,
T13184 9860-9864 NN denotes HygR
T13185 9872-9877 NNS denotes cells
T13186 9865-9866 SYM denotes +
T13187 9866-9867 HYPH denotes /
T13188 9867-9868 SYM denotes -
T13189 9869-9871 NN denotes ES
T13190 9877-9878 : denotes ;
T13191 9879-9880 SYM denotes -
T13192 9881-9882 SYM denotes -
T13193 9880-9881 HYPH denotes /
T13194 9882-9884 , denotes ,
T13195 9884-9888 NN denotes ESG1
T13196 9889-9893 JJ denotes null
T13197 9888-9889 HYPH denotes -
T13198 9897-9902 NNS denotes cells
T13199 9894-9896 NN denotes ES
T13200 9902-9903 . denotes .
T13201 9903-9935 sentence denotes Numbers indicate clone numbers.
T13202 9904-9911 NNS denotes Numbers
T13203 9912-9920 VBP denotes indicate
T13204 9921-9926 NN denotes clone
T13205 9927-9934 NNS denotes numbers
T13206 9934-9935 . denotes .
T13207 9935-10094 sentence denotes C) Northern blot (upper) and western blot (lower) analyses of wild-type ES cells (WT), ESG1-null ES cells (-/-, three clones) and heterozygous ES cells (+/-).
T13208 9936-9937 LS denotes C
T13209 9986-9994 NNS denotes analyses
T13210 9937-9938 -RRB- denotes )
T13211 9939-9947 NNP denotes Northern
T13212 9948-9952 NN denotes blot
T13213 9953-9954 -LRB- denotes (
T13214 9954-9959 JJ denotes upper
T13215 9959-9960 -RRB- denotes )
T13216 9961-9964 CC denotes and
T13217 9965-9972 NNP denotes western
T13218 9973-9977 NN denotes blot
T13219 9978-9979 -LRB- denotes (
T13220 9979-9984 JJ denotes lower
T13221 9984-9985 -RRB- denotes )
T13222 9995-9997 IN denotes of
T13223 9998-10002 JJ denotes wild
T13224 10003-10007 NN denotes type
T13225 10002-10003 HYPH denotes -
T13226 10011-10016 NNS denotes cells
T13227 10008-10010 NN denotes ES
T13228 10017-10018 -LRB- denotes (
T13229 10018-10020 NN denotes WT
T13230 10020-10021 -RRB- denotes )
T13231 10021-10023 , denotes ,
T13232 10023-10027 NN denotes ESG1
T13233 10028-10032 JJ denotes null
T13234 10027-10028 HYPH denotes -
T13235 10036-10041 NNS denotes cells
T13236 10033-10035 NN denotes ES
T13237 10042-10043 -LRB- denotes (
T13238 10054-10060 NNS denotes clones
T13239 10043-10044 SYM denotes -
T13240 10045-10046 SYM denotes -
T13241 10044-10045 HYPH denotes /
T13242 10046-10048 , denotes ,
T13243 10048-10053 CD denotes three
T13244 10060-10061 -RRB- denotes )
T13245 10062-10065 CC denotes and
T13246 10066-10078 JJ denotes heterozygous
T13247 10082-10087 NNS denotes cells
T13248 10079-10081 NN denotes ES
T13249 10088-10089 -LRB- denotes (
T13250 10091-10092 SYM denotes -
T13251 10089-10090 SYM denotes +
T13252 10090-10091 HYPH denotes /
T13253 10092-10093 -RRB- denotes )
T13254 10093-10094 . denotes .
T13255 10094-10152 sentence denotes Northern blot was performed as previously described [20].
T13256 10095-10103 NNP denotes Northern
T13257 10104-10108 NN denotes blot
T13258 10113-10122 VBN denotes performed
T13259 10109-10112 VBD denotes was
T13260 10123-10125 IN denotes as
T13261 10137-10146 VBN denotes described
T13262 10126-10136 RB denotes previously
T13263 10147-10148 -LRB- denotes [
T13264 10148-10150 CD denotes 20
T13265 10150-10151 -RRB- denotes ]
T13266 10151-10152 . denotes .
T13267 10152-10267 sentence denotes To confirm the loading of equal amounts of RNA, ethidium bromide staining of ribosomal RNA is also shown (middle).
T13268 10153-10155 TO denotes To
T13269 10156-10163 VB denotes confirm
T13270 10252-10257 VBN denotes shown
T13271 10164-10167 DT denotes the
T13272 10168-10175 NN denotes loading
T13273 10176-10178 IN denotes of
T13274 10179-10184 JJ denotes equal
T13275 10185-10192 NNS denotes amounts
T13276 10193-10195 IN denotes of
T13277 10196-10199 NN denotes RNA
T13278 10199-10201 , denotes ,
T13279 10201-10209 NN denotes ethidium
T13280 10210-10217 NN denotes bromide
T13281 10218-10226 NN denotes staining
T13282 10227-10229 IN denotes of
T13283 10230-10239 JJ denotes ribosomal
T13284 10240-10243 NN denotes RNA
T13285 10244-10246 VBZ denotes is
T13286 10247-10251 RB denotes also
T13287 10258-10259 -LRB- denotes (
T13288 10259-10265 JJ denotes middle
T13289 10265-10266 -RRB- denotes )
T13290 10266-10267 . denotes .
T13587 11321-11329 NNS denotes Analyses
T13588 11330-11332 IN denotes of
T13589 11333-11337 NN denotes ESG1
T13590 11338-11342 JJ denotes null
T13591 11337-11338 HYPH denotes -
T13592 11346-11351 NNS denotes cells
T13593 11343-11345 NN denotes ES
T13594 11351-11352 . denotes .
T13595 11352-11427 sentence denotes A) The morphology of ESG1-null ES cell colonies grown on STO feeder cells.
T13596 11353-11354 LS denotes A
T13597 11360-11370 NN denotes morphology
T13598 11354-11355 -RRB- denotes )
T13599 11356-11359 DT denotes The
T13600 11371-11373 IN denotes of
T13601 11374-11378 NN denotes ESG1
T13602 11379-11383 JJ denotes null
T13603 11378-11379 HYPH denotes -
T13604 11392-11400 NNS denotes colonies
T13605 11384-11386 NN denotes ES
T13606 11387-11391 NN denotes cell
T13607 11401-11406 VBN denotes grown
T13608 11407-11409 IN denotes on
T13609 11410-11413 NN denotes STO
T13610 11421-11426 NNS denotes cells
T13611 11414-11420 NN denotes feeder
T13612 11426-11427 . denotes .
T13613 11427-11511 sentence denotes B) Growth curve of wild-type (WT), ESG1-null (-/-) and heterozygous (+/-) ES cells.
T13614 11428-11429 LS denotes B
T13615 11438-11443 NN denotes curve
T13616 11429-11430 -RRB- denotes )
T13617 11431-11437 NN denotes Growth
T13618 11444-11446 IN denotes of
T13619 11447-11451 JJ denotes wild
T13620 11452-11456 NN denotes type
T13621 11451-11452 HYPH denotes -
T13622 11505-11510 NNS denotes cells
T13623 11457-11458 -LRB- denotes (
T13624 11458-11460 NN denotes WT
T13625 11460-11461 -RRB- denotes )
T13626 11461-11463 , denotes ,
T13627 11463-11467 NN denotes ESG1
T13628 11467-11468 HYPH denotes -
T13629 11468-11472 JJ denotes null
T13630 11473-11474 -LRB- denotes (
T13631 11474-11475 SYM denotes -
T13632 11476-11477 SYM denotes -
T13633 11475-11476 HYPH denotes /
T13634 11477-11478 -RRB- denotes )
T13635 11479-11482 CC denotes and
T13636 11483-11495 JJ denotes heterozygous
T13637 11496-11497 -LRB- denotes (
T13638 11497-11498 SYM denotes +
T13639 11499-11500 SYM denotes -
T13640 11498-11499 HYPH denotes /
T13641 11500-11501 -RRB- denotes )
T13642 11502-11504 NN denotes ES
T13643 11510-11511 . denotes .
T13644 11511-11573 sentence denotes Each clone (1 × 104 cells/well) was plated in 24-well plates.
T13645 11512-11516 DT denotes Each
T13646 11517-11522 NN denotes clone
T13647 11548-11554 VBN denotes plated
T13648 11523-11524 -LRB- denotes (
T13649 11532-11537 NNS denotes cells
T13650 11524-11525 CD denotes 1
T13651 11528-11531 CD denotes 104
T13652 11526-11527 SYM denotes ×
T13653 11537-11538 SYM denotes /
T13654 11538-11542 NN denotes well
T13655 11542-11543 -RRB- denotes )
T13656 11544-11547 VBD denotes was
T13657 11555-11557 IN denotes in
T13658 11558-11560 CD denotes 24
T13659 11561-11565 NN denotes well
T13660 11560-11561 HYPH denotes -
T13661 11566-11572 NNS denotes plates
T13662 11572-11573 . denotes .
T13663 11573-11646 sentence denotes Cell numbers were determined with a Coulter counter at 2, 4, and 6 days.
T13664 11574-11578 NN denotes Cell
T13665 11579-11586 NNS denotes numbers
T13666 11592-11602 VBN denotes determined
T13667 11587-11591 VBD denotes were
T13668 11603-11607 IN denotes with
T13669 11608-11609 DT denotes a
T13670 11618-11625 NN denotes counter
T13671 11610-11617 NNP denotes Coulter
T13672 11626-11628 IN denotes at
T13673 11629-11630 CD denotes 2
T13674 11641-11645 NNS denotes days
T13675 11630-11632 , denotes ,
T13676 11632-11633 CD denotes 4
T13677 11633-11635 , denotes ,
T13678 11635-11638 CC denotes and
T13679 11639-11640 CD denotes 6
T13680 11645-11646 . denotes .
T13681 11646-11747 sentence denotes Data of +/- and -/- cells are shown as averages and standard deviations of three independent clones.
T13682 11647-11651 NNS denotes Data
T13683 11677-11682 VBN denotes shown
T13684 11652-11654 IN denotes of
T13685 11655-11656 SYM denotes +
T13686 11657-11658 SYM denotes -
T13687 11656-11657 HYPH denotes /
T13688 11667-11672 NNS denotes cells
T13689 11659-11662 CC denotes and
T13690 11663-11664 SYM denotes -
T13691 11665-11666 SYM denotes -
T13692 11664-11665 HYPH denotes /
T13693 11673-11676 VBP denotes are
T13694 11683-11685 IN denotes as
T13695 11686-11694 NNS denotes averages
T13696 11695-11698 CC denotes and
T13697 11699-11707 JJ denotes standard
T13698 11708-11718 NNS denotes deviations
T13699 11719-11721 IN denotes of
T13700 11722-11727 CD denotes three
T13701 11740-11746 NNS denotes clones
T13702 11728-11739 JJ denotes independent
T13703 11746-11747 . denotes .
T13704 11747-11836 sentence denotes C) A section of teratoma derived from ESG1-null ES cells (hematoxylin & eosin staining).
T13705 11748-11749 LS denotes C
T13706 11753-11760 NN denotes section
T13707 11749-11750 -RRB- denotes )
T13708 11751-11752 DT denotes A
T13709 11761-11763 IN denotes of
T13710 11764-11772 NN denotes teratoma
T13711 11773-11780 VBN denotes derived
T13712 11781-11785 IN denotes from
T13713 11786-11790 NN denotes ESG1
T13714 11791-11795 JJ denotes null
T13715 11790-11791 HYPH denotes -
T13716 11799-11804 NNS denotes cells
T13717 11796-11798 NN denotes ES
T13718 11805-11806 -LRB- denotes (
T13719 11826-11834 NN denotes staining
T13720 11806-11817 NN denotes hematoxylin
T13721 11818-11819 CC denotes &
T13722 11820-11825 NN denotes eosin
T13723 11834-11835 -RRB- denotes )
T13724 11835-11836 . denotes .
T13961 12728-12732 NN denotes Gene
T13962 12733-12743 NN denotes expression
T13963 12744-12752 NNS denotes analyses
T13964 12753-12755 IN denotes of
T13965 12756-12760 NN denotes ESG1
T13966 12761-12765 JJ denotes null
T13967 12760-12761 HYPH denotes -
T13968 12769-12774 NNS denotes cells
T13969 12766-12768 NN denotes ES
T13970 12774-12775 . denotes .
T13971 12775-12803 sentence denotes A) DNA microarray analyses.
T13972 12776-12777 LS denotes A
T13973 12794-12802 NNS denotes analyses
T13974 12777-12778 -RRB- denotes )
T13975 12779-12782 NN denotes DNA
T13976 12783-12793 NN denotes microarray
T13977 12802-12803 . denotes .
T13978 12803-12905 sentence denotes Total RNA from wild-type ES cells and ESG1-null ES cells were labeled with Cy3 and Cy5, respectively.
T13979 12804-12809 JJ denotes Total
T13980 12810-12813 NN denotes RNA
T13981 12866-12873 VBN denotes labeled
T13982 12814-12818 IN denotes from
T13983 12819-12823 JJ denotes wild
T13984 12824-12828 NN denotes type
T13985 12823-12824 HYPH denotes -
T13986 12832-12837 NNS denotes cells
T13987 12829-12831 NN denotes ES
T13988 12838-12841 CC denotes and
T13989 12842-12846 NN denotes ESG1
T13990 12847-12851 JJ denotes null
T13991 12846-12847 HYPH denotes -
T13992 12855-12860 NNS denotes cells
T13993 12852-12854 NN denotes ES
T13994 12861-12865 VBD denotes were
T13995 12874-12878 IN denotes with
T13996 12879-12882 NN denotes Cy3
T13997 12883-12886 CC denotes and
T13998 12887-12890 NN denotes Cy5
T13999 12890-12892 , denotes ,
T14000 12892-12904 RB denotes respectively
T14001 12904-12905 . denotes .
T14002 12905-12973 sentence denotes Samples were hybridized to Agilent Mouse Developmental Microarrays.
T14003 12906-12913 NNS denotes Samples
T14004 12919-12929 VBN denotes hybridized
T14005 12914-12918 VBD denotes were
T14006 12930-12932 IN denotes to
T14007 12933-12940 NNP denotes Agilent
T14008 12961-12972 NNS denotes Microarrays
T14009 12941-12946 NN denotes Mouse
T14010 12947-12960 JJ denotes Developmental
T14011 12972-12973 . denotes .
T14012 12973-13023 sentence denotes The averages of two independent clones are shown.
T14013 12974-12977 DT denotes The
T14014 12978-12986 NNS denotes averages
T14015 13017-13022 VBN denotes shown
T14016 12987-12989 IN denotes of
T14017 12990-12993 CD denotes two
T14018 13006-13012 NNS denotes clones
T14019 12994-13005 JJ denotes independent
T14020 13013-13016 VBP denotes are
T14021 13022-13023 . denotes .
T14022 13023-13049 sentence denotes B) Western blot analyses.
T14023 13024-13025 LS denotes B
T14024 13040-13048 NNS denotes analyses
T14025 13025-13026 -RRB- denotes )
T14026 13027-13034 NNP denotes Western
T14027 13035-13039 NN denotes blot
T14028 13048-13049 . denotes .
T14029 13049-13168 sentence denotes Cell lysates from ESG1+/- and ESG1-/- cells were examined for expression of ESG1, Oct3/4 and CDK4 with immunoblotting.
T14030 13050-13054 NN denotes Cell
T14031 13055-13062 NNS denotes lysates
T14032 13099-13107 VBN denotes examined
T14033 13063-13067 IN denotes from
T14034 13068-13072 NN denotes ESG1
T14035 13088-13093 NNS denotes cells
T14036 13072-13073 SYM denotes +
T14037 13073-13074 HYPH denotes /
T14038 13074-13075 SYM denotes -
T14039 13076-13079 CC denotes and
T14040 13080-13084 NN denotes ESG1
T14041 13084-13085 SYM denotes -
T14042 13085-13086 HYPH denotes /
T14043 13086-13087 SYM denotes -
T14044 13094-13098 VBD denotes were
T14045 13108-13111 IN denotes for
T14046 13112-13122 NN denotes expression
T14047 13123-13125 IN denotes of
T14048 13126-13130 NN denotes ESG1
T14049 13130-13132 , denotes ,
T14050 13132-13136 NN denotes Oct3
T14051 13136-13137 HYPH denotes /
T14052 13137-13138 CD denotes 4
T14053 13139-13142 CC denotes and
T14054 13143-13147 NN denotes CDK4
T14055 13148-13152 IN denotes with
T14056 13153-13167 NN denotes immunoblotting
T14057 13167-13168 . denotes .
T4103 4676-4678 IN denotes in
T4104 4679-4682 NN denotes DNA
T4105 4683-4692 NNS denotes fragments
T4106 4693-4696 IN denotes for
T4107 4732-4740 VBD denotes remained
T4108 4697-4702 WDT denotes which
T4109 4703-4706 DT denotes the
T4110 4719-4731 NN denotes localization
T4111 4707-4718 JJ denotes chromosomal
T4112 4741-4749 JJ denotes unmapped
T4113 4749-4750 . denotes .
T4114 4750-4890 sentence denotes While these pseudodgenes have significant homology to ESG1 cDNA, they could not produce functional proteins, because of critical mutations.
T4115 4751-4756 IN denotes While
T4116 4776-4780 VBP denotes have
T4117 4757-4762 DT denotes these
T4118 4763-4775 NNS denotes pseudodgenes
T4119 4831-4838 VB denotes produce
T4120 4781-4792 JJ denotes significant
T4121 4793-4801 NN denotes homology
T4183 5381-5388 JJ denotes similar
T4184 5389-5391 IN denotes to
T4185 5392-5395 DT denotes the
T4186 5401-5405 NN denotes gene
T4386 6434-6435 . denotes .
T4387 6435-6620 sentence denotes The 5' flanking region of this DNA fragment exhibited strong promoter/enhancer activity by luciferase reporter assays in undifferentiated ES cells, but not in somatic cells (Figure 3).
T4388 6436-6439 DT denotes The
T4389 6452-6458 NN denotes region
T4390 6440-6441 CD denotes 5
T4391 6441-6442 SYM denotes '
T4392 6443-6451 NN denotes flanking
T4393 6480-6489 VBD denotes exhibited
T4394 6459-6461 IN denotes of
T4477 7741-7742 DT denotes a
T4478 7761-7769 NN denotes sequence
T4479 7743-7745 CD denotes 68
T4480 7746-7749 NN denotes kbp
T4481 7750-7760 JJ denotes intergenic
T4482 7769-7771 , denotes ,
T4483 7771-7775 VBD denotes were
T4484 7785-7787 IN denotes in
T4485 7788-7796 JJ denotes opposite
T4486 7797-7807 NNS denotes directions
T6502 9084-9086 NN denotes 4A
T6503 9077-9083 NN denotes Figure
T6504 9086-9087 -RRB- denotes )
T6505 9088-9098 VBN denotes introduced
T6506 9099-9103 IN denotes into
T6507 9104-9107 NN denotes RF8
T6508 9111-9116 NNS denotes cells
T6509 9108-9110 NN denotes ES
T6510 9117-9119 IN denotes by
T6511 9120-9135 NN denotes electroporation
T6512 9135-9136 . denotes .
T6513 9136-9297 sentence denotes We obtained eight ES cell clones with correct homologous recombination of the β-geo targeting vector, which was confirmed by Southern blot analysis (Figure 4B).
T6514 9137-9139 PRP denotes We
T6515 9140-9148 VBD denotes obtained
T6516 9149-9154 CD denotes eight
T6573 10280-10282 sentence denotes mo
T6574 10280-10281 VBG denotes m
T6575 10281-10282 JJ denotes o
T6576 10282-10289 sentence denotes zygous
T6607 10377-10381 NN denotes G418
T6608 10382-10391 JJ denotes resistant
T6609 10381-10382 HYPH denotes -
T6610 10401-10407 VBN denotes tested
T6729 10953-10958 IN denotes after
T6730 10959-10962 DT denotes the
T6731 10963-10970 NN denotes removal
T6732 10971-10973 IN denotes of
T6733 10974-10982 NN denotes leukemia
T6734 10994-11000 NN denotes factor
T6735 10983-10993 JJ denotes inhibitory
T6736 11001-11002 -LRB- denotes (
T6737 11009-11011 NN denotes 5B
T6798 11837-11841 sentence denotes We e
T6799 11840-11841 NNS denotes e
T6800 11837-11838 NN denotes W
T6801 11838-11839 JJ denotes e
T6802 11841-11843 sentence denotes xa
T6810 11858-11868 NN denotes expression
T6811 11878-11880 IN denotes of
T6812 11881-11885 NN denotes ESG1
T6813 11892-11897 NNS denotes cells
T6814 11885-11886 SYM denotes -
T7006 13175-13176 NN denotes e
T7007 13178-13181 sentence denotes te
T7008 13178-13179 CD denotes t
T7009 13179-13180 JJ denotes e
T7010 13181-13186 sentence denotes ESG1-
T7011 13181-13185 NN denotes ESG1
T7012 13185-13186 SYM denotes -
T7013 13186-13278 sentence denotes knockout mice, we injected β-geo-ESG1+/- ES cell clones into the blastocysts of C57BL6 mice.
T7014 13204-13212 VBD denotes injected
T7015 13186-13194 NN denotes knockout
T802 0-14 NN denotes Identification
T4095 4619-4621 IN denotes In
T4096 4665-4675 VBN denotes identified
T4097 4622-4630 NN denotes addition
T4098 4630-4632 , denotes ,
T4099 4632-4635 CD denotes two
T4100 4648-4659 NNS denotes pseudogenes
T4101 4636-4647 JJ denotes intron-less
T4102 4660-4664 VBD denotes were
T4173 5335-5441 sentence denotes On chromosome 9, we identified a DNA fragment similar to the ESG1 gene that included two putative introns.
T4174 5335-5337 IN denotes On
T4175 5355-5365 VBD denotes identified
T4176 5338-5348 NN denotes chromosome
T4177 5349-5350 CD denotes 9
T4178 5350-5352 , denotes ,
T4179 5352-5354 PRP denotes we
T4180 5366-5367 DT denotes a
T4181 5372-5380 NN denotes fragment
T4182 5368-5371 NN denotes DNA
T4370 6375-6377 NN denotes AG
T4371 6377-6379 , denotes ,
T4372 6379-6389 VBG denotes fulfilling
T4373 6390-6393 DT denotes the
T4374 6400-6404 NN denotes rule
T4375 6394-6396 NN denotes GT
T4376 6397-6399 NN denotes AG
T4377 6396-6397 HYPH denotes -
T4378 6405-6407 IN denotes of
T4379 6408-6412 NN denotes exon
T4380 6413-6419 NN denotes intron
T4381 6412-6413 HYPH denotes -
T4382 6420-6429 NNS denotes junctions
T4383 6430-6431 -LRB- denotes [
T4384 6431-6433 CD denotes 19
T4385 6433-6434 -RRB- denotes ]
T4449 6769-7708 sentence denotes Figure 2 BAC clones containing the ESG1 gene and a duplication pseudogene (PS). A) Localization of the gene and PS on chromosome 9. B) Sequence comparison of the gene and PS revealed that these are homologous from eight base pairs upstream of the first exon to 675 bp downstream of the third exon. Substitution mutations are indicated by black lines. The insertion is indicated by an open triangle. Figure 3 Promoter/enhancer activity of the ESG1 gene and pseudogene. DNA fragments of ~6 kbp isolated from the 5' flanking regions of the gene and PS were transferred into luciferase reporter plasmids. We introduced the reporter genes into undifferentiated ES cells (open columns), retinoic acid-treated ES cells (grey columns), and NIH3T3 cells (closed columns). Data represent the averages and standard deviations of three independent experiments. We also found that the two BAC clones contained another ESG1-like sequence (Figure 2A).
T4450 7621-7623 PRP denotes We
T4451 7629-7634 VBD denotes found
T4452 7624-7628 RB denotes also
T4453 7635-7639 IN denotes that
T4454 7659-7668 VBD denotes contained
T4455 7640-7643 DT denotes the
T4456 7652-7658 NNS denotes clones
T4457 7644-7647 CD denotes two
T4458 7648-7651 NN denotes BAC
T4459 7669-7676 DT denotes another
T4460 7687-7695 NN denotes sequence
T4461 7677-7681 NN denotes ESG1
T4462 7682-7686 JJ denotes like
T4463 7681-7682 HYPH denotes -
T4464 7696-7697 -LRB- denotes (
T4465 7704-7706 NN denotes 2A
T4466 7697-7703 NN denotes Figure
T4467 7706-7707 -RRB- denotes )
T4468 7707-7708 . denotes .
T4469 7708-7808 sentence denotes The two sequences, separated by a 68 kbp intergenic sequence, were oriented in opposite directions.
T4470 7709-7712 DT denotes The
T4471 7717-7726 NNS denotes sequences
T4472 7713-7716 CD denotes two
T4473 7776-7784 VBN denotes oriented
T4474 7726-7728 , denotes ,
T4475 7728-7737 VBN denotes separated
T4476 7738-7740 IN denotes by
T6492 9015-9025 NN denotes hygromycin
T6493 9026-9035 JJ denotes resistant
T6494 9041-9042 -LRB- denotes (
T6495 9042-9046 NN denotes HygR
T6496 9046-9047 -RRB- denotes )
T6497 9048-9053 VBG denotes using
T6498 9054-9057 CD denotes two
T6499 9068-9075 NNS denotes vectors
T6500 9058-9067 NN denotes targeting
T6501 9076-9077 -LRB- denotes (
T6571 10275-10276 WDT denotes i
T6572 10276-10277 NNP denotes n
T6577 10282-10288 JJ denotes zygous
T6578 10289-10291 sentence denotes mu
T6579 10289-10290 VBN denotes m
T6580 10290-10291 RB denotes u
T6581 10291-10296 sentence denotes tant
T6582 10291-10292 DT denotes t
T6583 10292-10294 NN denotes an
T6584 10294-10295 NNS denotes t
T6585 10296-10369 sentence denotes ES cells, we introduced the β-geo vector into HygR heterozygous ES cells.
T6586 10309-10319 VBD denotes introduced
T6587 10299-10304 NNS denotes cells
T6588 10296-10298 NN denotes ES
T6589 10304-10306 , denotes ,
T6590 10306-10308 PRP denotes we
T6591 10320-10323 DT denotes the
T6592 10330-10336 NN denotes vector
T6593 10324-10325 NN denotes β
T6594 10326-10329 NN denotes geo
T6595 10325-10326 HYPH denotes -
T6596 10337-10341 IN denotes into
T6597 10342-10346 NN denotes HygR
T6598 10347-10359 JJ denotes heterozygous
T6599 10363-10368 NNS denotes cells
T6600 10360-10362 NN denotes ES
T6601 10368-10369 . denotes .
T6602 10369-10446 sentence denotes Of 105 G418-resistant colonies tested, 49 were homozygous for ESG1 deletion.
T6603 10370-10372 IN denotes Of
T6604 10412-10416 VBD denotes were
T6605 10373-10376 CD denotes 105
T6606 10392-10400 NNS denotes colonies
T6716 10902-10903 -LRB- denotes (
T6717 10910-10912 NN denotes 5B
T6718 10903-10909 NN denotes Figure
T6719 10912-10913 -RRB- denotes )
T6720 10913-10914 . denotes .
T6721 10914-11052 sentence denotes ESG1-/- cells differentiated normally after the removal of leukemia inhibitory factor (Figure 5B) or retinoic acid treatment (not shown).
T6722 10915-10919 NN denotes ESG1
T6723 10923-10928 NNS denotes cells
T6724 10919-10920 SYM denotes -
T6725 10920-10921 HYPH denotes /
T6726 10921-10922 SYM denotes -
T6727 10929-10943 VBD denotes differentiated
T6728 10944-10952 RB denotes normally
T6803 11842-11843 NN denotes a
T6804 11841-11842 NN denotes x
T6805 11843-11969 sentence denotes mined the gene expression profiles of ESG1-/- ES cells using oligonucleotide-based DNA microarrays representing ~20,000 genes.
T6806 11843-11848 VBD denotes mined
T6807 11849-11852 DT denotes the
T6808 11869-11877 NNS denotes profiles
T6809 11853-11857 NN denotes gene
T6896 12240-12247 NN denotes protein
T7001 13169-13172 sentence denotes To
T7002 13169-13171 JJ denotes To
T7003 13172-13178 sentence denotes genera
T7004 13172-13175 NNP denotes gen
T7005 13176-13178 NNS denotes ra
T7016 13195-13199 NNS denotes mice
R27 T824 T825 amod Embryonic,cell
R11 T804 T802 cc and,Identification
R12 T805 T806 amod targeted,disruption
R13 T806 T802 conj disruption,Identification
R14 T807 T802 prep of,Identification
R15 T808 T809 det the,gene
R16 T809 T807 pobj gene,of
R17 T810 T809 compound mouse,gene
R18 T811 T809 acl encoding,gene
R19 T812 T811 dobj ESG1,encoding
R20 T813 T812 punct (,ESG1
R21 T814 T815 compound PH34,DPPA5
R22 T815 T812 appos DPPA5,ESG1
R23 T816 T815 punct /,DPPA5
R24 T817 T815 compound ECAT2,DPPA5
R25 T818 T815 punct /,DPPA5
R26 T819 T802 punct ),Identification
R28 T825 T827 npadvmod cell,specific
R29 T826 T825 compound stem,cell
R30 T827 T829 amod specific,gene
R31 T828 T827 punct -,specific
R32 T829 T830 nsubjpass gene,expressed
R33 T831 T832 punct (,ESG
R34 T832 T829 parataxis ESG,gene
R35 T833 T832 punct ),ESG
R36 T834 T829 nummod 1,gene
R37 T835 T829 punct ", ",gene
R38 T836 T837 dep which,encodes
R39 T837 T829 relcl encodes,gene
R40 T838 T839 det a,protein
R41 T839 T837 dobj protein,encodes
R42 T840 T841 compound KH,domain
R43 T841 T843 npadvmod domain,containing
R44 T842 T841 punct -,domain
R45 T843 T839 amod containing,protein
R46 T844 T830 punct ", ",expressed
R47 T845 T830 auxpass is,expressed
R48 T846 T830 advmod specifically,expressed
R49 T847 T830 prep in,expressed
R50 T848 T849 amod early,embryos
R51 T849 T847 pobj embryos,in
R52 T850 T849 punct ", ",embryos
R53 T851 T852 compound germ,cells
R54 T852 T849 conj cells,embryos
R55 T853 T852 punct ", ",cells
R56 T854 T852 cc and,cells
R57 T855 T856 amod embryonic,stem
R58 T856 T857 nmod stem,cells
R59 T857 T852 conj cells,cells
R60 T858 T856 punct (,stem
R61 T859 T856 appos ES,stem
R62 T860 T857 punct ),cells
R63 T861 T830 punct .,expressed
R64 T863 T864 amod Previous,studies
R65 T864 T865 nsubj studies,identified
R66 T866 T867 amod genomic,clones
R67 T867 T865 dobj clones,identified
R68 T868 T867 acl containing,clones
R69 T869 T870 det the,gene
R70 T870 T868 dobj gene,containing
R71 T871 T870 compound mouse,gene
R72 T872 T870 compound ESG1,gene
R73 T873 T870 cc and,gene
R74 T874 T875 nummod five,pseudogenes
R75 T875 T870 conj pseudogenes,gene
R76 T876 T865 punct .,identified
R77 T878 T879 advmod However,determined
R78 T880 T879 punct ", ",determined
R79 T881 T882 poss their,localizations
R80 T882 T879 nsubjpass localizations,determined
R81 T883 T882 amod chromosomal,localizations
R82 T884 T882 cc or,localizations
R83 T885 T886 amod physiological,functions
R84 T886 T882 conj functions,localizations
R85 T887 T879 aux have,determined
R86 T888 T879 neg not,determined
R87 T889 T879 auxpass been,determined
R88 T890 T879 punct .,determined
R89 T894 T895 det A,search
R90 T895 T897 nsubj search,failed
R91 T896 T895 compound Blast,search
R92 T898 T895 prep of,search
R93 T899 T900 nmod mouse,databases
R94 T900 T898 pobj databases,of
R95 T901 T900 amod genomic,databases
R96 T902 T903 aux to,locate
R97 T903 T897 xcomp locate,failed
R98 T904 T905 det the,gene
R99 T905 T903 dobj gene,locate
R100 T906 T905 compound ESG1,gene
R101 T907 T897 punct .,failed
R102 T909 T910 nsubj We,identified
R103 T911 T912 amod several,clones
R104 T912 T910 dobj clones,identified
R105 T913 T912 amod bacterial,clones
R106 T914 T912 amod artificial,clones
R107 T915 T912 acl containing,clones
R108 T916 T917 det the,gene
R109 T917 T915 dobj gene,containing
R110 T918 T917 compound mouse,gene
R111 T919 T917 compound ESG1,gene
R112 T920 T912 cc and,clones
R113 T921 T922 det an,sequence
R114 T922 T912 conj sequence,clones
R115 T923 T922 amod additional,sequence
R116 T924 T925 npadvmod ESG1,like
R117 T925 T922 amod like,sequence
R118 T926 T925 punct -,like
R119 T927 T922 prep with,sequence
R120 T928 T929 det a,structure
R121 T929 T927 pobj structure,with
R122 T930 T929 amod similar,structure
R123 T931 T929 compound gene,structure
R124 T932 T929 prep from,structure
R125 T933 T932 pobj chromosome,from
R126 T934 T933 nummod 9,chromosome
R127 T935 T910 punct .,identified
R128 T937 T938 det The,sequence
R129 T938 T942 nsubj sequence,contained
R130 T939 T940 npadvmod ESG1,like
R131 T940 T938 amod like,sequence
R132 T941 T940 punct -,like
R133 T943 T944 det a,mutations
R134 T944 T942 dobj mutations,contained
R135 T945 T944 amod multiple,mutations
R136 T946 T944 amod critical,mutations
R137 T947 T942 punct ", ",contained
R138 T948 T942 advcl indicating,contained
R139 T949 T950 mark that,was
R140 T950 T948 ccomp was,indicating
R141 T951 T950 nsubj it,was
R142 T952 T953 det a,pseudogene
R143 T953 T950 attr pseudogene,was
R144 T954 T953 amod duplicated,pseudogene
R145 T955 T942 punct .,contained
R146 T957 T958 det The,region
R147 T958 T962 nsubj region,exhibited
R148 T959 T958 nummod 5,region
R149 T960 T959 punct ',5
R150 T961 T958 compound flanking,region
R151 T963 T958 prep of,region
R152 T964 T965 det the,gene
R153 T965 T963 pobj gene,of
R154 T966 T965 compound ESG1,gene
R155 T967 T965 punct ", ",gene
R156 T968 T965 cc but,gene
R157 T969 T968 neg not,but
R158 T970 T965 conj that,gene
R159 T971 T970 prep of,that
R160 T972 T973 det the,pseudogene
R161 T973 T971 pobj pseudogene,of
R162 T974 T962 punct ", ",exhibited
R163 T975 T976 amod strong,activity
R164 T976 T962 dobj activity,exhibited
R165 T977 T976 nmod enhancer,activity
R166 T978 T977 cc and,enhancer
R167 T979 T977 conj promoter,enhancer
R168 T980 T962 prep in,exhibited
R169 T981 T982 amod undifferentiated,cells
R170 T982 T980 pobj cells,in
R171 T983 T982 compound ES,cells
R172 T984 T962 prep by,exhibited
R173 T985 T986 compound luciferase,reporter
R174 T986 T987 compound reporter,assay
R175 T987 T984 pobj assay,by
R176 T988 T962 punct .,exhibited
R177 T990 T991 aux To,study
R178 T991 T992 advcl study,replaced
R179 T993 T994 det the,functions
R180 T994 T991 dobj functions,study
R181 T995 T994 amod physiological,functions
R182 T996 T994 prep of,functions
R183 T997 T998 det the,gene
R184 T998 T996 pobj gene,of
R185 T999 T998 compound ESG1,gene
R186 T1000 T992 punct ", ",replaced
R187 T1001 T992 nsubj we,replaced
R188 T1002 T1003 det this,sequence
R189 T1003 T992 dobj sequence,replaced
R190 T1004 T1003 prep in,sequence
R191 T1005 T1006 compound ES,cells
R192 T1006 T1004 pobj cells,in
R193 T1007 T992 prep with,replaced
R194 T1008 T1009 det a,cassette
R195 T1009 T1007 pobj cassette,with
R196 T1010 T1011 compound β,geo
R197 T1011 T1009 compound geo,cassette
R198 T1012 T1011 punct -,geo
R199 T1013 T992 prep by,replaced
R200 T1014 T1015 amod homologous,recombination
R201 T1015 T1013 pobj recombination,by
R202 T1016 T992 punct .,replaced
R203 T1018 T1019 prep Despite,developed
R204 T1020 T1021 amod specific,expression
R205 T1021 T1018 pobj expression,Despite
R206 T1022 T1021 prep in,expression
R207 T1023 T1024 amod early,embryos
R208 T1024 T1022 pobj embryos,in
R209 T1025 T1024 cc and,embryos
R210 T1026 T1027 compound germ,cells
R211 T1027 T1024 conj cells,embryos
R212 T1028 T1019 punct ", ",developed
R213 T1029 T1030 nmod ESG1,mice
R214 T1030 T1019 nsubj mice,developed
R215 T1031 T1029 punct -,ESG1
R216 T1032 T1029 punct /,ESG1
R217 T1033 T1029 punct -,ESG1
R218 T1034 T1019 advmod normally,developed
R219 T1035 T1019 cc and,developed
R220 T1036 T1019 conj were,developed
R221 T1037 T1036 acomp fertile,were
R222 T1038 T1019 punct .,developed
R223 T1040 T1041 nsubj We,generated
R224 T1042 T1041 advmod also,generated
R225 T1043 T1044 nmod ESG1,cells
R226 T1044 T1041 dobj cells,generated
R227 T1045 T1043 punct -,ESG1
R228 T1046 T1043 punct /,ESG1
R229 T1047 T1043 punct -,ESG1
R230 T1048 T1044 compound ES,cells
R231 T1049 T1050 preconj both,by
R232 T1050 T1041 prep by,generated
R233 T1051 T1052 det a,recombination
R234 T1052 T1050 pobj recombination,by
R235 T1053 T1052 amod second,recombination
R236 T1054 T1052 amod independent,recombination
R237 T1055 T1052 amod homologous,recombination
R238 T1056 T1050 cc and,by
R239 T1057 T1058 advmod directly,from
R240 T1058 T1050 conj from,by
R241 T1059 T1058 pobj blastocysts,from
R242 T1060 T1059 acl derived,blastocysts
R243 T1061 T1060 prep from,derived
R244 T1062 T1063 amod heterozygous,intercrosses
R245 T1063 T1061 pobj intercrosses,from
R246 T1064 T1041 punct .,generated
R247 T1066 T1067 nmod Northern,blot
R248 T1067 T1068 nmod blot,analyses
R249 T1068 T1072 nsubj analyses,confirmed
R250 T1069 T1067 cc and,blot
R251 T1070 T1071 compound western,blot
R252 T1071 T1067 conj blot,blot
R253 T1073 T1074 det the,absence
R254 T1074 T1072 dobj absence,confirmed
R255 T1075 T1074 prep of,absence
R256 T1076 T1075 pobj ESG1,of
R257 T1077 T1074 prep in,absence
R258 T1078 T1079 det these,cells
R259 T1079 T1077 pobj cells,in
R260 T1080 T1072 punct .,confirmed
R261 T1082 T1083 det These,cells
R262 T1083 T1085 nsubj cells,demonstrated
R263 T1084 T1083 compound ES,cells
R264 T1086 T1087 amod normal,morphology
R265 T1087 T1085 dobj morphology,demonstrated
R266 T1088 T1087 punct ", ",morphology
R267 T1089 T1087 conj proliferation,morphology
R268 T1090 T1089 punct ", ",proliferation
R269 T1091 T1089 cc and,proliferation
R270 T1092 T1089 conj differentiation,proliferation
R271 T1093 T1085 punct .,demonstrated
R272 T1097 T1098 det The,gene
R273 T1098 T1101 nsubjpass gene,located
R274 T1099 T1098 compound mouse,gene
R275 T1100 T1098 compound ESG1,gene
R276 T1102 T1098 punct ", ",gene
R277 T1103 T1104 advmod together,with
R278 T1104 T1098 prep with,gene
R279 T1105 T1106 det a,pseudogene
R280 T1106 T1104 pobj pseudogene,with
R281 T1107 T1106 amod duplicated,pseudogene
R282 T1108 T1101 punct ", ",located
R283 T1109 T1101 auxpass is,located
R284 T1110 T1101 prep on,located
R285 T1111 T1110 pobj chromosome,on
R286 T1112 T1111 nummod 9,chromosome
R287 T1113 T1101 punct .,located
R288 T1115 T1116 prep Despite,is
R289 T1117 T1118 poss its,expression
R290 T1118 T1115 pobj expression,Despite
R291 T1119 T1118 amod specific,expression
R292 T1120 T1118 prep in,expression
R293 T1121 T1122 amod pluripotent,cells
R294 T1122 T1120 pobj cells,in
R295 T1123 T1122 cc and,cells
R296 T1124 T1125 compound germ,cells
R297 T1125 T1122 conj cells,cells
R298 T1126 T1116 punct ", ",is
R299 T1127 T1116 nsubj ESG1,is
R300 T1128 T1116 acomp dispensable,is
R301 T1129 T1128 prep for,dispensable
R302 T1130 T1131 compound self,renewal
R303 T1131 T1129 pobj renewal,for
R304 T1132 T1131 punct -,renewal
R305 T1133 T1131 prep of,renewal
R306 T1134 T1135 compound ES,cells
R307 T1135 T1133 pobj cells,of
R308 T1136 T1131 cc and,renewal
R309 T1137 T1131 conj establishment,renewal
R310 T1138 T1137 prep of,establishment
R311 T1139 T1138 pobj germcells,of
R312 T1140 T1116 punct .,is
R325 T2208 T2209 amod Embryonic,stem
R326 T2209 T2210 nmod stem,cells
R327 T2210 T2214 nsubjpass cells,derived
R328 T2211 T2209 punct (,stem
R329 T2212 T2209 appos ES,stem
R330 T2213 T2210 punct ),cells
R331 T2215 T2214 auxpass were,derived
R332 T2216 T2214 advmod first,derived
R333 T2217 T2214 prep from,derived
R334 T2218 T2219 det the,blastocysts
R335 T2219 T2217 pobj blastocysts,from
R336 T2220 T2219 prep of,blastocysts
R337 T2221 T2220 pobj mice,of
R338 T2222 T2214 prep in,derived
R339 T2223 T2222 pobj 1981,in
R340 T2224 T2225 punct [,2
R341 T2225 T2223 parataxis 2,1981
R342 T2226 T2225 nummod 1,2
R343 T2227 T2225 punct ",",2
R344 T2228 T2225 punct ],2
R345 T2229 T2214 cc and,derived
R346 T2230 T2214 conj humans,derived
R347 T2231 T2230 prep in,humans
R348 T2232 T2231 pobj 1998,in
R349 T2233 T2234 punct [,3
R350 T2234 T2232 parataxis 3,1998
R351 T2235 T2234 punct ],3
R352 T2236 T2214 punct .,derived
R353 T2238 T2239 compound ES,cells
R354 T2239 T2240 nsubj cells,have
R355 T2241 T2242 nummod two,properties
R356 T2242 T2240 dobj properties,have
R357 T2243 T2242 amod important,properties
R358 T2244 T2242 punct : ,properties
R359 T2245 T2242 appos theability,properties
R360 T2246 T2247 aux to,maintain
R361 T2247 T2245 acl maintain,theability
R362 T2248 T2247 dobj pluripotency,maintain
R363 T2249 T2248 punct ", ",pluripotency
R364 T2250 T2251 dep which,is
R365 T2251 T2248 relcl is,pluripotency
R366 T2252 T2253 det the,ability
R367 T2253 T2251 attr ability,is
R368 T2254 T2255 aux to,differentiate
R369 T2255 T2253 acl differentiate,ability
R370 T2256 T2255 prep into,differentiate
R371 T2257 T2258 det a,variety
R372 T2258 T2256 pobj variety,into
R373 T2259 T2258 amod wide,variety
R374 T2260 T2258 prep of,variety
R375 T2261 T2260 pobj cells,of
R376 T2262 T2245 punct ", ",theability
R377 T2263 T2245 cc and,theability
R378 T2264 T2265 amod rapid,proliferation
R379 T2265 T2245 conj proliferation,theability
R380 T2266 T2240 punct .,have
R381 T2268 T2269 det These,characteristics
R382 T2269 T2270 nsubj characteristics,make
R383 T2271 T2272 compound mouse,cells
R384 T2272 T2274 nsubj cells,component
R385 T2273 T2272 compound ES,cells
R386 T2274 T2270 ccomp component,make
R387 T2275 T2274 det an,component
R388 T2276 T2274 amod essential,component
R389 T2277 T2274 prep of,component
R390 T2278 T2279 compound gene,targeting
R391 T2279 T2280 compound targeting,technology
R392 T2280 T2277 pobj technology,of
R393 T2281 T2270 punct .,make
R394 T2283 T2284 det These,qualities
R395 T2284 T2285 nsubj qualities,make
R396 T2286 T2285 advmod also,make
R397 T2287 T2288 amod human,cells
R398 T2288 T2290 nsubj cells,sources
R399 T2289 T2288 compound ES,cells
R400 T2290 T2285 ccomp sources,make
R401 T2291 T2290 amod attractive,sources
R402 T2292 T2290 prep for,sources
R403 T2293 T2294 compound cell,transplantation
R404 T2294 T2295 compound transplantation,therapy
R405 T2295 T2292 pobj therapy,for
R406 T2296 T2297 aux to,treat
R407 T2297 T2295 advcl treat,therapy
R408 T2298 T2299 amod various,diseases
R409 T2299 T2297 dobj diseases,treat
R410 T2300 T2299 punct ", ",diseases
R411 T2301 T2299 prep including,diseases
R412 T2302 T2303 amod spinal,cord
R413 T2303 T2304 compound cord,injuries
R414 T2304 T2301 pobj injuries,including
R415 T2305 T2304 cc and,injuries
R416 T2306 T2307 amod juvenile,diabetes
R417 T2307 T2304 conj diabetes,injuries
R418 T2308 T2285 punct .,make
R419 T2310 T2311 det The,mechanisms
R420 T2311 T2313 nsubj mechanisms,are
R421 T2312 T2311 amod molecular,mechanisms
R422 T2314 T2311 acl underlying,mechanisms
R423 T2315 T2316 det the,pluripotency
R424 T2316 T2314 dobj pluripotency,underlying
R425 T2317 T2316 cc and,pluripotency
R426 T2318 T2319 amod rapid,proliferation
R427 T2319 T2316 conj proliferation,pluripotency
R428 T2320 T2316 prep of,pluripotency
R429 T2321 T2322 compound ES,cells
R430 T2322 T2320 pobj cells,of
R431 T2323 T2313 advmod currently,are
R432 T2324 T2325 det a,focus
R433 T2325 T2313 attr focus,are
R434 T2326 T2325 amod major,focus
R435 T2327 T2325 prep of,focus
R436 T2328 T2329 det the,field
R437 T2329 T2327 pobj field,of
R438 T2330 T2329 prep of,field
R439 T2331 T2332 compound stem,cell
R440 T2332 T2333 compound cell,biology
R441 T2333 T2330 pobj biology,of
R442 T2334 T2335 punct [,4
R443 T2335 T2313 parataxis 4,are
R444 T2336 T2337 punct -,6
R445 T2337 T2335 prep 6,4
R446 T2338 T2335 punct ],4
R447 T2339 T2313 punct .,are
R448 T2341 T2342 aux To,identify
R449 T2342 T2343 advcl identify,utilized
R450 T2344 T2342 dobj molecules,identify
R451 T2345 T2344 relcl essential,molecules
R452 T2346 T2345 prep in,essential
R453 T2347 T2348 compound ES,cells
R454 T2348 T2346 pobj cells,in
R455 T2349 T2345 prep for,essential
R456 T2350 T2351 det these,properties
R457 T2351 T2349 pobj properties,for
R458 T2352 T2343 punct ", ",utilized
R459 T2353 T2354 amod several,groups
R460 T2354 T2343 nsubj groups,utilized
R461 T2355 T2343 aux have,utilized
R462 T2356 T2357 compound transcriptome,analyses
R463 T2357 T2343 dobj analyses,utilized
R464 T2358 T2359 aux to,identify
R465 T2359 T2343 advcl identify,utilized
R466 T2360 T2359 dobj genes,identify
R467 T2361 T2362 advmod specifically,expressed
R468 T2362 T2360 acl expressed,genes
R469 T2363 T2362 prep in,expressed
R470 T2364 T2365 compound ES,cells
R471 T2365 T2363 pobj cells,in
R472 T2366 T2365 cc and,cells
R473 T2367 T2368 amod early,embryos
R474 T2368 T2365 conj embryos,cells
R475 T2369 T2343 punct .,utilized
R476 T2371 T2372 det These,analyses
R477 T2372 T2373 nsubj analyses,identified
R478 T2374 T2372 punct ", ",analyses
R479 T2375 T2372 prep including,analyses
R480 T2376 T2377 compound DNA,analyses
R481 T2377 T2375 pobj analyses,including
R482 T2378 T2377 compound microarray,analyses
R483 T2379 T2380 punct [,7
R484 T2380 T2377 parataxis 7,analyses
R485 T2381 T2380 punct ],7
R486 T2382 T2377 cc and,analyses
R487 T2383 T2384 amod expressed,sequence
R488 T2384 T2385 compound sequence,analyses
R489 T2385 T2377 conj analyses,analyses
R490 T2386 T2385 compound tag,analyses
R491 T2387 T2388 punct [,8
R492 T2388 T2385 parataxis 8,analyses
R493 T2389 T2390 punct -,12
R494 T2390 T2388 prep 12,8
R495 T2391 T2388 punct ],8
R496 T2392 T2373 punct ", ",identified
R497 T2393 T2394 amod several,transcripts
R498 T2394 T2373 dobj transcripts,identified
R499 T2395 T2394 amod common,transcripts
R500 T2396 T2394 punct ", ",transcripts
R501 T2397 T2394 prep including,transcripts
R502 T2398 T2397 pobj ESG1,including
R503 T2399 T2400 dep that,designated
R504 T2400 T2394 relcl designated,transcripts
R505 T2401 T2400 auxpass was,designated
R506 T2402 T2400 advmod also,designated
R507 T2403 T2400 oprd dppa5,designated
R508 T2404 T2403 cc or,dppa5
R509 T2405 T2403 conj ECAT2,dppa5
R510 T2406 T2373 punct .,identified
R511 T2408 T2409 nsubjpass ESG1,identified
R512 T2410 T2409 auxpass was,identified
R513 T2411 T2409 advmod originally,identified
R514 T2412 T2409 prep as,identified
R515 T2413 T2414 det a,Ph34
R516 T2414 T2412 pobj Ph34,as
R517 T2415 T2414 compound transcript,Ph34
R518 T2416 T2417 dep that,regulated
R519 T2417 T2414 relcl regulated,Ph34
R520 T2418 T2417 auxpass was,regulated
R521 T2419 T2417 advmod down,regulated
R522 T2420 T2417 punct -,regulated
R523 T2421 T2417 agent by,regulated
R524 T2422 T2423 amod retinoic,acid
R525 T2423 T2421 pobj acid,by
R526 T2424 T2417 prep in,regulated
R527 T2425 T2426 amod embryonic,carcinoma
R528 T2426 T2427 compound carcinoma,cells
R529 T2427 T2424 pobj cells,in
R530 T2428 T2429 punct [,13
R531 T2429 T2417 parataxis 13,regulated
R532 T2430 T2429 punct ],13
R533 T2431 T2409 punct .,identified
R534 T2433 T2434 det The,expression
R535 T2434 T2435 nsubjpass expression,confined
R536 T2436 T2434 prep of,expression
R537 T2437 T2438 det this,gene
R538 T2438 T2436 pobj gene,of
R539 T2439 T2435 auxpass was,confined
R540 T2440 T2435 prep in,confined
R541 T2441 T2440 pobj mice,in
R542 T2442 T2435 prep to,confined
R543 T2443 T2444 amod early,embryos
R544 T2444 T2442 pobj embryos,to
R545 T2445 T2444 cc and,embryos
R546 T2446 T2447 compound germ,cells
R547 T2447 T2444 conj cells,embryos
R548 T2448 T2449 punct [,14
R549 T2449 T2435 parataxis 14,confined
R550 T2450 T2449 punct ],14
R551 T2451 T2435 punct .,confined
R552 T2453 T2454 nsubjpass It,expressed
R553 T2455 T2454 auxpass is,expressed
R554 T2456 T2454 advmod also,expressed
R555 T2457 T2454 prep in,expressed
R556 T2458 T2459 amod pluripotent,cells
R557 T2459 T2457 pobj cells,in
R558 T2460 T2459 punct ", ",cells
R559 T2461 T2459 prep including,cells
R560 T2462 T2463 compound ES,cells
R561 T2463 T2461 pobj cells,including
R562 T2464 T2463 punct ", ",cells
R563 T2465 T2466 amod embryonic,cells
R564 T2466 T2463 conj cells,cells
R565 T2467 T2466 compound germ,cells
R566 T2468 T2466 punct ", ",cells
R567 T2469 T2466 cc and,cells
R568 T2470 T2471 amod multipotent,cells
R569 T2471 T2466 conj cells,cells
R570 T2472 T2471 compound germline,cells
R571 T2473 T2471 compound stem,cells
R572 T2474 T2475 punct [,15
R573 T2475 T2454 parataxis 15,expressed
R574 T2476 T2475 punct ],15
R575 T2477 T2454 punct .,expressed
R576 T2479 T2480 nsubj ESG1,encodes
R577 T2481 T2482 det a,polypeptide
R578 T2482 T2480 dobj polypeptide,encodes
R579 T2483 T2482 prep of,polypeptide
R580 T2484 T2485 nummod 118,acids
R581 T2485 T2483 pobj acids,of
R582 T2486 T2485 compound amino,acids
R583 T2487 T2488 dep that,contains
R584 T2488 T2482 relcl contains,polypeptide
R585 T2489 T2490 det a,domain
R586 T2490 T2488 dobj domain,contains
R587 T2491 T2490 amod single,domain
R588 T2492 T2490 compound KH,domain
R589 T2493 T2490 punct ", ",domain
R590 T2494 T2495 dep which,is
R591 T2495 T2490 relcl is,domain
R592 T2496 T2497 det an,domain
R593 T2497 T2495 attr domain,is
R594 T2498 T2499 npadvmod RNA,binding
R595 T2499 T2497 amod binding,domain
R596 T2500 T2499 punct -,binding
R597 T2501 T2502 punct [,16
R598 T2502 T2495 parataxis 16,is
R599 T2503 T2502 punct ],16
R600 T2504 T2480 punct .,encodes
R601 T2506 T2507 nsubj It,remains
R602 T2508 T2507 acomp unclear,remains
R603 T2509 T2507 punct ", ",remains
R604 T2510 T2507 advmod however,remains
R605 T2511 T2507 punct ", ",remains
R606 T2512 T2513 mark if,functions
R607 T2513 T2507 ccomp functions,remains
R608 T2514 T2513 nsubj ESG1,functions
R609 T2515 T2513 prep as,functions
R610 T2516 T2517 det an,protein
R611 T2517 T2515 pobj protein,as
R612 T2518 T2519 npadvmod RNA,binding
R613 T2519 T2517 amod binding,protein
R614 T2520 T2519 punct -,binding
R615 T2521 T2513 cc or,functions
R616 T2522 T2523 det the,roles
R617 T2523 T2513 conj roles,functions
R618 T2524 T2525 nsubj it,plays
R619 T2525 T2523 advcl plays,roles
R620 T2526 T2525 prep in,plays
R621 T2527 T2528 compound ES,cells
R622 T2528 T2526 pobj cells,in
R623 T2529 T2528 cc and,cells
R624 T2530 T2528 conj mice,cells
R625 T2531 T2507 punct .,remains
R626 T2533 T2534 amod Previous,screening
R627 T2535 T2536 amod genomic,library
R628 T2536 T2534 compound library,screening
R629 T2537 T2534 prep by,screening
R630 T2538 T2539 amod identified,clones
R631 T2539 T2537 pobj clones,by
R632 T2540 T2539 amod genomic,clones
R633 T2541 T2539 acl containing,clones
R634 T2542 T2543 det the,gene
R635 T2543 T2541 dobj gene,containing
R636 T2544 T2543 compound mouse,gene
R637 T2545 T2543 compound ESG1,gene
R638 T2546 T2543 cc and,gene
R639 T2547 T2548 nummod seven,pseudogenes
R640 T2548 T2543 conj pseudogenes,gene
R641 T2549 T2550 punct [,17
R642 T2550 T2541 parataxis 17,containing
R643 T2551 T2550 punct ],17
R644 T2552 T2534 punct .,screening
R645 T2554 T2555 nsubj Two,exhibit
R646 T2556 T2554 prep of,Two
R647 T2557 T2558 det these,pseudogenes
R648 T2558 T2556 pobj pseudogenes,of
R649 T2559 T2560 det a,structure
R650 T2560 T2555 dobj structure,exhibit
R651 T2561 T2560 amod similar,structure
R652 T2562 T2563 compound exon,intron
R653 T2563 T2560 compound intron,structure
R654 T2564 T2563 punct -,intron
R655 T2565 T2560 prep as,structure
R656 T2566 T2567 det the,gene
R657 T2567 T2565 pobj gene,as
R658 T2568 T2567 compound ESG1,gene
R659 T2569 T2555 punct ", ",exhibit
R660 T2570 T2555 advcl indicating,exhibit
R661 T2571 T2572 poss their,generation
R662 T2572 T2570 dobj generation,indicating
R663 T2573 T2570 prep by,indicating
R664 T2574 T2575 compound gene,duplication
R665 T2575 T2573 pobj duplication,by
R666 T2576 T2555 punct .,exhibit
R667 T2578 T2579 det The,pseudogenes
R668 T2579 T2582 nsubj pseudogenes,contain
R669 T2580 T2579 nummod five,pseudogenes
R670 T2581 T2579 amod remaining,pseudogenes
R671 T2583 T2582 aux did,contain
R672 T2584 T2582 neg not,contain
R673 T2585 T2586 det any,introns
R674 T2586 T2582 dobj introns,contain
R675 T2587 T2582 punct ", ",contain
R676 T2588 T2582 advcl indicating,contain
R677 T2589 T2590 mark that,generated
R678 T2590 T2588 ccomp generated,indicating
R679 T2591 T2590 nsubjpass these,generated
R680 T2592 T2590 auxpass were,generated
R681 T2593 T2590 agent by,generated
R682 T2594 T2593 pobj retrotransposition,by
R683 T2595 T2594 prep of,retrotransposition
R684 T2596 T2597 compound ESG1,transcripts
R685 T2597 T2595 pobj transcripts,of
R686 T2598 T2582 punct .,contain
R687 T2600 T2601 det The,localizations
R688 T2601 T2603 nsubjpass localizations,reported
R689 T2602 T2601 amod chromosomal,localizations
R690 T2604 T2601 prep of,localizations
R691 T2605 T2606 det the,gene
R692 T2606 T2604 pobj gene,of
R693 T2607 T2606 compound mouse,gene
R694 T2608 T2606 compound ESG1,gene
R695 T2609 T2606 cc and,gene
R696 T2610 T2606 conj pseudogenes,gene
R697 T2611 T2603 punct ", ",reported
R698 T2612 T2603 advmod however,reported
R699 T2613 T2603 punct ", ",reported
R700 T2614 T2603 aux have,reported
R701 T2615 T2603 neg not,reported
R702 T2616 T2603 auxpass been,reported
R703 T2617 T2603 punct .,reported
R704 T2619 T2620 prep In,determined
R705 T2621 T2622 det this,study
R706 T2622 T2619 pobj study,In
R707 T2623 T2620 punct ", ",determined
R708 T2624 T2620 nsubj we,determined
R709 T2625 T2626 det the,structure
R710 T2626 T2620 dobj structure,determined
R711 T2627 T2626 prep of,structure
R712 T2628 T2629 det the,gene
R713 T2629 T2627 pobj gene,of
R714 T2630 T2629 compound mouse,gene
R715 T2631 T2629 acl encoding,gene
R716 T2632 T2633 det this,protein
R717 T2633 T2631 dobj protein,encoding
R718 T2634 T2633 cc and,protein
R719 T2635 T2636 amod related,pseudogenes
R720 T2636 T2633 conj pseudogenes,protein
R721 T2637 T2620 punct .,determined
R722 T2639 T2640 nsubj We,performed
R723 T2641 T2640 advmod also,performed
R724 T2642 T2643 compound gene,targeting
R725 T2643 T2640 dobj targeting,performed
R726 T2644 T2645 aux to,determine
R727 T2645 T2640 advcl determine,performed
R728 T2646 T2647 det the,function
R729 T2647 T2645 dobj function,determine
R730 T2648 T2647 amod physiological,function
R731 T2649 T2647 prep of,function
R732 T2650 T2649 pobj ESG1,of
R733 T2651 T2640 punct .,performed
R734 T4020 T4021 amod Chromosomal,localization
R735 T4022 T4021 cc and,localization
R736 T4023 T4021 conj structures,localization
R737 T4024 T4021 prep of,localization
R738 T4025 T4026 compound mouse,gene
R739 T4026 T4024 pobj gene,of
R740 T4027 T4026 compound ESG1,gene
R741 T4028 T4026 cc and,gene
R742 T4029 T4026 conj psedogenes,gene
R743 T4031 T4032 aux To,determine
R744 T4032 T4033 advcl determine,performed
R745 T4034 T4035 det the,localizations
R746 T4035 T4032 dobj localizations,determine
R747 T4036 T4035 amod chromosomal,localizations
R748 T4037 T4035 prep of,localizations
R749 T4038 T4039 det the,gene
R750 T4039 T4037 pobj gene,of
R751 T4040 T4039 compound mouse,gene
R752 T4041 T4039 compound ESG1,gene
R753 T4042 T4039 cc and,gene
R754 T4043 T4039 conj pseudogenes,gene
R755 T4044 T4033 punct ", ",performed
R756 T4045 T4033 nsubj we,performed
R757 T4046 T4047 det a,analysis
R758 T4047 T4033 dobj analysis,performed
R759 T4048 T4047 compound Blast,analysis
R760 T4049 T4047 prep of,analysis
R761 T4050 T4051 det the,database
R762 T4051 T4049 pobj database,of
R763 T4052 T4051 nmod mouse,database
R764 T4053 T4051 amod genomic,database
R765 T4054 T4033 prep with,performed
R766 T4055 T4056 det the,sequence
R767 T4056 T4054 pobj sequence,with
R768 T4057 T4056 compound ESG1,sequence
R769 T4058 T4056 compound cDNA,sequence
R770 T4059 T4056 prep as,sequence
R771 T4060 T4061 det a,query
R772 T4061 T4059 pobj query,as
R773 T4062 T4033 punct .,performed
R774 T4064 T4065 nsubj We,identified
R775 T4066 T4067 amod several,pseudogenes
R776 T4067 T4065 dobj pseudogenes,identified
R777 T4068 T4067 amod putative,pseudogenes
R778 T4069 T4067 prep without,pseudogenes
R779 T4070 T4069 pobj introns,without
R780 T4071 T4065 prep on,identified
R781 T4072 T4073 nmod chromosomes,X
R782 T4073 T4071 pobj X,on
R783 T4074 T4073 nummod 1,X
R784 T4075 T4073 punct ", ",X
R785 T4076 T4073 nummod 5,X
R786 T4077 T4073 punct ", ",X
R787 T4078 T4073 nummod 11,X
R788 T4079 T4073 punct ", ",X
R789 T4080 T4073 nummod 12,X
R790 T4081 T4073 punct ", ",X
R791 T4082 T4073 nummod 14,X
R792 T4083 T4073 punct ", ",X
R793 T4084 T4073 nummod 16,X
R794 T4085 T4073 punct ", ",X
R795 T4086 T4073 nummod 17,X
R796 T4087 T4073 punct ", ",X
R797 T4088 T4073 cc and,X
R798 T4089 T4090 punct (,Figure
R799 T4090 T4065 parataxis Figure,identified
R800 T4091 T4090 nummod 1,Figure
R801 T4092 T4090 punct ),Figure
R802 T4093 T4065 punct .,identified
R803 T4095 T4096 prep In,identified
R804 T4097 T4095 pobj addition,In
R805 T4098 T4096 punct ", ",identified
R806 T4099 T4100 nummod two,pseudogenes
R807 T4100 T4096 nsubjpass pseudogenes,identified
R808 T4101 T4100 amod intron-less,pseudogenes
R809 T4102 T4096 auxpass were,identified
R810 T4103 T4096 prep in,identified
R811 T4104 T4105 compound DNA,fragments
R812 T4105 T4103 pobj fragments,in
R813 T4106 T4107 prep for,remained
R814 T4107 T4105 relcl remained,fragments
R815 T4108 T4106 pobj which,for
R816 T4109 T4110 det the,localization
R817 T4110 T4107 nsubj localization,remained
R818 T4111 T4110 amod chromosomal,localization
R819 T4112 T4107 acomp unmapped,remained
R820 T4113 T4096 punct .,identified
R821 T4115 T4116 mark While,have
R822 T4116 T4119 advcl have,produce
R823 T4117 T4118 det these,pseudodgenes
R824 T4118 T4116 nsubj pseudodgenes,have
R825 T4120 T4121 amod significant,homology
R826 T4121 T4116 dobj homology,have
R827 T4122 T4121 prep to,homology
R862 T4161 T4156 attr hallmark,is
R863 T4162 T4161 prep of,hallmark
R864 T4163 T4164 amod pluripotent,genes
R865 T4164 T4162 pobj genes,of
R866 T4165 T4166 npadvmod cell,specific
R867 T4166 T4164 amod specific,genes
R868 T4167 T4166 punct -,specific
R869 T4168 T4169 punct [,18
R870 T4169 T4161 parataxis 18,hallmark
R871 T4170 T4169 punct ],18
R872 T4171 T4156 punct .,is
R873 T4174 T4175 prep On,identified
R874 T4176 T4174 pobj chromosome,On
R875 T4177 T4176 nummod 9,chromosome
R876 T4178 T4175 punct ", ",identified
R877 T4179 T4175 nsubj we,identified
R878 T4180 T4181 det a,fragment
R879 T4181 T4175 dobj fragment,identified
R880 T4182 T4181 compound DNA,fragment
R881 T4183 T4181 amod similar,fragment
R882 T4184 T4183 prep to,similar
R883 T4185 T4186 det the,gene
R884 T4186 T4184 pobj gene,to
R885 T4187 T4186 compound ESG1,gene
R886 T4188 T4189 dep that,included
R887 T4189 T4186 relcl included,gene
R888 T4190 T4191 nummod two,introns
R889 T4191 T4189 dobj introns,included
R890 T4192 T4191 amod putative,introns
R891 T4193 T4175 punct .,identified
R892 T4195 T4196 det These,exons
R893 T4196 T4201 nsubj exons,contained
R894 T4197 T4196 amod putative,exons
R895 T4198 T4196 amod first,exons
R896 T4199 T4198 cc and,first
R897 T4200 T4198 conj second,first
R898 T4202 T4201 punct ", ",contained
R899 T4203 T4201 advmod however,contained
R900 T4204 T4201 punct ", ",contained
R901 T4205 T4206 punct (,4
R902 T4206 T4201 parataxis 4,contained
R903 T4207 T4206 punct ),4
R904 T4208 T4209 amod multiple,mutations
R905 T4209 T4201 dobj mutations,contained
R906 T4210 T4209 prep of,mutations
R907 T4211 T4212 det the,sequence
R908 T4212 T4210 pobj sequence,of
R909 T4213 T4212 compound ESG1,sequence
R910 T4214 T4212 compound cDNA,sequence
R911 T4215 T4201 punct .,contained
R912 T4217 T4218 det The,exon
R913 T4218 T4221 nsubj exon,was
R914 T4219 T4218 amod putative,exon
R915 T4220 T4218 amod third,exon
R916 T4222 T4221 acomp identical,was
R917 T4223 T4222 prep to,identical
R918 T4224 T4223 pobj that,to
R919 T4225 T4224 prep of,that
R920 T4226 T4227 det the,gene
R921 T4227 T4225 pobj gene,of
R922 T4228 T4229 advmod previously,reported
R923 T4229 T4227 amod reported,gene
R924 T4230 T4227 compound ESG1,gene
R925 T4231 T4221 punct .,was
R926 T4233 T4234 advmod Also,identified
R927 T4235 T4234 prep on,identified
R928 T4236 T4235 pobj chromosome,on
R929 T4237 T4236 nummod 9,chromosome
R930 T4238 T4234 punct ", ",identified
R931 T4239 T4234 nsubj we,identified
R932 T4240 T4241 det another,fragment
R933 T4241 T4234 dobj fragment,identified
R934 T4242 T4241 compound DNA,fragment
R935 T4243 T4244 dep that,was
R936 T4244 T4241 relcl was,fragment
R937 T4245 T4244 acomp similar,was
R938 T4246 T4245 punct ", ",similar
R939 T4247 T4245 cc but,similar
R940 T4248 T4247 neg not,but
R941 T4249 T4245 conj identical,similar
R942 T4250 T4249 punct ", ",identical
R943 T4251 T4249 conj to,identical
R944 T4252 T4253 det the,exon
R945 T4253 T4251 pobj exon,to
R946 T4254 T4253 amod third,exon
R947 T4255 T4253 prep of,exon
R948 T4256 T4257 det the,gene
R949 T4257 T4255 pobj gene,of
R950 T4258 T4257 compound ESG1,gene
R951 T4259 T4234 punct .,identified
R952 T4261 T4262 det These,findings
R953 T4262 T4263 nsubj findings,suggest
R954 T4264 T4265 mark that,assembled
R955 T4265 T4263 ccomp assembled,suggest
R956 T4266 T4267 det these,sequences
R957 T4267 T4265 nsubjpass sequences,assembled
R958 T4268 T4269 npadvmod ESG1,like
R959 T4269 T4267 amod like,sequences
R960 T4270 T4269 punct -,like
R961 T4271 T4267 prep on,sequences
R962 T4272 T4271 pobj chromosome,on
R963 T4273 T4272 nummod 9,chromosome
R964 T4274 T4265 aux have,assembled
R965 T4275 T4265 neg not,assembled
R966 T4276 T4265 auxpass been,assembled
R967 T4277 T4265 advmod correctly,assembled
R968 T4278 T4263 punct .,suggest
R969 T4280 T4281 aux To,obtain
R970 T4281 T4282 advcl obtain,screened
R971 T4283 T4284 compound DNA,fragments
R972 T4284 T4281 dobj fragments,obtain
R973 T4285 T4284 acl containing,fragments
R974 T4286 T4287 det the,gene
R975 T4287 T4285 dobj gene,containing
R976 T4288 T4287 compound ESG1,gene
R977 T4289 T4282 punct ", ",screened
R978 T4290 T4282 nsubj we,screened
R979 T4291 T4292 det the,pool
R980 T4292 T4282 dobj pool,screened
R981 T4293 T4294 amod bacterial,chromosome
R982 T4294 T4292 nmod chromosome,pool
R983 T4295 T4294 amod artificial,chromosome
R984 T4296 T4294 punct (,chromosome
R985 T4297 T4294 appos BAC,chromosome
R986 T4298 T4292 punct ),pool
R987 T4299 T4292 compound DNA,pool
R988 T4300 T4282 prep by,screened
R989 T4301 T4300 pobj PCR,by
R990 T4302 T4282 advcl using,screened
R991 T4303 T4304 compound primer,pairs
R992 T4304 T4302 dobj pairs,using
R993 T4305 T4306 dep that,amplify
R994 T4306 T4304 relcl amplify,pairs
R995 T4307 T4306 aux would,amplify
R996 T4308 T4306 advmod only,amplify
R997 T4309 T4310 det the,gene
R998 T4310 T4306 dobj gene,amplify
R999 T4311 T4310 amod real,gene
R1000 T4312 T4306 punct ", ",amplify
R1001 T4313 T4314 neg not,pseudogenes
R1002 T4314 T4306 dep pseudogenes,amplify
R1003 T4315 T4314 det the,pseudogenes
R1004 T4316 T4282 punct .,screened
R1005 T4318 T4319 nsubj We,obtained
R1006 T4320 T4321 nummod two,clones
R1007 T4321 T4319 dobj clones,obtained
R1008 T4322 T4321 amod independent,clones
R1009 T4323 T4322 punct ", ",independent
R1010 T4324 T4322 cc but,independent
R1011 T4325 T4322 conj overlapping,independent
R1012 T4326 T4321 compound BAC,clones
R1013 T4327 T4319 punct .,obtained
R1014 T4329 T4330 compound Southern,blot
R1015 T4330 T4331 nsubj blot,demonstrated
R1016 T4332 T4330 appos analyses,blot
R1017 T4333 T4332 cc and,analyses
R1018 T4334 T4332 conj sequencing,analyses
R1019 T4335 T4336 mark that,contained
R1020 T4336 T4331 ccomp contained,demonstrated
R1021 T4337 T4338 det these,clones
R1022 T4338 T4336 nsubj clones,contained
R1023 T4339 T4340 det a,sequence
R1024 T4340 T4336 dobj sequence,contained
R1025 T4341 T4340 acl exhibiting,sequence
R1026 T4342 T4343 amod complete,identity
R1027 T4343 T4341 dobj identity,exhibiting
R1028 T4344 T4343 prep with,identity
R1029 T4345 T4346 compound ESG1,cDNA
R1030 T4346 T4344 pobj cDNA,with
R1031 T4347 T4348 dep that,interrupted
R1032 T4348 T4340 relcl interrupted,sequence
R1033 T4349 T4348 auxpass was,interrupted
R1034 T4350 T4348 agent by,interrupted
R1035 T4351 T4352 nummod two,introns
R1036 T4352 T4350 pobj introns,by
R1037 T4353 T4352 amod putative,introns
R1038 T4354 T4355 punct (,2A
R1039 T4355 T4348 parataxis 2A,interrupted
R1040 T4356 T4355 compound Figure,2A
R1041 T4357 T4355 punct ),2A
R1042 T4358 T4331 punct .,demonstrated
R1043 T4360 T4361 det The,sequences
R1044 T4361 T4364 nsubj sequences,begin
R1045 T4362 T4361 nummod two,sequences
R1046 T4363 T4361 compound intron,sequences
R1047 T4365 T4364 prep with,begin
R1048 T4366 T4365 pobj GT,with
R1049 T4367 T4364 cc and,begin
R1050 T4368 T4364 conj terminate,begin
R1051 T4369 T4368 prep with,terminate
R1052 T4370 T4369 pobj AG,with
R1053 T4371 T4364 punct ", ",begin
R1054 T4372 T4364 advcl fulfilling,begin
R1055 T4373 T4374 det the,rule
R1056 T4374 T4372 dobj rule,fulfilling
R1057 T4375 T4376 compound GT,AG
R1058 T4376 T4374 compound AG,rule
R1059 T4377 T4376 punct -,AG
R1060 T4378 T4374 prep of,rule
R1061 T4379 T4380 compound exon,intron
R1062 T4380 T4382 compound intron,junctions
R1063 T4381 T4380 punct -,intron
R1064 T4382 T4378 pobj junctions,of
R1065 T4383 T4384 punct [,19
R1066 T4384 T4372 parataxis 19,fulfilling
R1067 T4385 T4384 punct ],19
R1068 T4386 T4364 punct .,begin
R1069 T4388 T4389 det The,region
R1070 T4389 T4393 nsubj region,exhibited
R1071 T4390 T4389 nummod 5,region
R1072 T4391 T4390 punct ',5
R1073 T4392 T4389 compound flanking,region
R1074 T4394 T4389 prep of,region
R1075 T4395 T4396 det this,fragment
R1076 T4396 T4394 pobj fragment,of
R1077 T4397 T4396 compound DNA,fragment
R1078 T4398 T4399 amod strong,activity
R1079 T4399 T4393 dobj activity,exhibited
R1080 T4400 T4401 compound promoter,enhancer
R1081 T4401 T4399 compound enhancer,activity
R1082 T4402 T4401 punct /,enhancer
R1083 T4403 T4393 prep by,exhibited
R1084 T4404 T4405 compound luciferase,assays
R1085 T4405 T4403 pobj assays,by
R1086 T4406 T4405 compound reporter,assays
R1087 T4407 T4393 prep in,exhibited
R1088 T4408 T4409 amod undifferentiated,cells
R1089 T4409 T4407 pobj cells,in
R1090 T4410 T4409 compound ES,cells
R1091 T4411 T4407 punct ", ",in
R1092 T4412 T4407 cc but,in
R1093 T4413 T4412 neg not,but
R1094 T4414 T4407 conj in,in
R1095 T4415 T4416 amod somatic,cells
R1096 T4416 T4414 pobj cells,in
R1097 T4417 T4418 punct (,Figure
R1098 T4418 T4393 parataxis Figure,exhibited
R1099 T4419 T4418 nummod 3,Figure
R1100 T4420 T4418 punct ),Figure
R1101 T4421 T4393 punct .,exhibited
R1102 T4423 T4424 det The,fragment
R1103 T4424 T4426 nsubj fragment,showed
R1104 T4425 T4424 amod same,fragment
R1105 T4427 T4428 advmod much,weaker
R1106 T4428 T4429 amod weaker,activity
R1107 T4429 T4426 dobj activity,showed
R1108 T4430 T4426 prep after,showed
R1109 T4431 T4430 pobj induction,after
R1110 T4432 T4431 prep of,induction
R1111 T4433 T4432 pobj differentiation,of
R1112 T4434 T4431 prep by,induction
R1113 T4435 T4436 amod retinoic,acid
R1114 T4436 T4434 pobj acid,by
R1115 T4437 T4426 punct .,showed
R1116 T4439 T4440 nsubj We,concluded
R1117 T4441 T4442 mark that,is
R1125 T4515 T4513 advmod however,contained
R1126 T4516 T4513 punct ", ",contained
R1127 T4517 T4518 amod critical,substitutions
R1128 T4518 T4513 dobj substitutions,contained
R1129 T4519 T4518 compound nucleotide,substitutions
R1130 T4520 T4513 prep in,contained
R1131 T4521 T4520 pobj all,in
R1132 T4522 T4521 prep of,all
R1133 T4523 T4524 det the,exons
R1134 T4524 T4522 pobj exons,of
R1135 T4525 T4513 cc and,contained
R1136 T4526 T4527 nummod one,insertion
R1137 T4527 T4513 conj insertion,contained
R1138 T4528 T4527 compound nucleotide,insertion
R1139 T4529 T4527 prep in,insertion
R1140 T4530 T4529 pobj exon,in
R1142 T4531 T4530 nummod 2,exon
R1143 T4532 T4533 punct (,2B
R1146 T4533 T4527 parataxis 2B,insertion
R1147 T4534 T4533 compound Figure,2B
R1148 T4454 T4451 ccomp contained,found
R1149 T4535 T4533 punct ),2B
R1151 T4456 T4454 nsubj clones,contained
R1152 T4536 T4513 punct .,contained
R1155 T4459 T4460 det another,sequence
R1156 T4460 T4454 dobj sequence,contained
R1157 T4461 T4462 npadvmod ESG1,like
R1158 T4462 T4460 amod like,sequence
R1159 T4538 T4539 mark Although,conserved
R1160 T4463 T4462 punct -,like
R1161 T4464 T4465 punct (,2A
R1162 T4465 T4454 parataxis 2A,contained
R1163 T4539 T4550 advcl conserved,were
R1164 T4466 T4465 compound Figure,2A
R1165 T4467 T4465 punct ),2A
R1166 T4468 T4451 punct .,found
R1167 T4540 T4541 nummod 675,pairs
R1168 T4470 T4471 det The,sequences
R1169 T4471 T4473 nsubjpass sequences,oriented
R1170 T4541 T4539 nsubjpass pairs,conserved
R1171 T4472 T4471 nummod two,sequences
R1172 T4474 T4471 punct ", ",sequences
R1173 T4542 T4541 compound base,pairs
R1174 T4475 T4471 acl separated,sequences
R1175 T4476 T4475 agent by,separated
R1176 T4543 T4541 prep of,pairs
R1177 T4477 T4478 det a,sequence
R1178 T4478 T4476 pobj sequence,by
R1179 T4479 T4480 nummod 68,kbp
R1180 T4544 T4545 det the,regions
R1181 T4480 T4478 nmod kbp,sequence
R1182 T4481 T4478 amod intergenic,sequence
R1183 T4545 T4543 pobj regions,of
R1184 T4482 T4473 punct ", ",oriented
R1185 T4546 T4545 nummod 3,regions
R1186 T4483 T4473 auxpass were,oriented
R1187 T4484 T4473 prep in,oriented
R1188 T4485 T4486 amod opposite,directions
R1189 T4486 T4484 pobj directions,in
R1190 T4487 T4473 punct .,oriented
R1191 T4547 T4546 punct ',3
R1192 T4489 T4490 det The,sequence
R1193 T4490 T4494 nsubj sequence,exhibited
R1194 T4548 T4545 compound flanking,regions
R1195 T4491 T4492 npadvmod ESG1,like
R1196 T4492 T4490 amod like,sequence
R1197 T4493 T4492 punct -,like
R1198 T4549 T4539 auxpass were,conserved
R1199 T4495 T4496 amod greater,95
R1200 T4496 T4498 nummod 95,%
R1201 T4551 T4539 prep between,conserved
R1202 T4497 T4496 quantmod than,95
R1203 T4498 T4499 compound %,identity
R1204 T4499 T4494 dobj identity,exhibited
R1205 T4500 T4499 prep to,identity
R1206 T4552 T4553 det the,gene
R1207 T4501 T4502 det the,exons
R1208 T4502 T4500 pobj exons,to
R1209 T4503 T4502 cc and,exons
R1210 T4504 T4502 conj introns,exons
R1211 T4505 T4502 prep of,exons
R1212 T4553 T4551 pobj gene,between
R1213 T4506 T4507 det the,gene
R1214 T4507 T4505 pobj gene,of
R1215 T4508 T4507 compound ESG1,gene
R1216 T4554 T4553 compound ESG1,gene
R1217 T4509 T4494 punct .,exhibited
R1218 T4511 T4512 det This,sequence
R1219 T4555 T4553 cc and,gene
R1220 T4512 T4513 nsubj sequence,contained
R1221 T4556 T4557 det the,pseudogene
R1222 T4514 T4513 punct ", ",contained
R1223 T4557 T4553 conj pseudogene,gene
R1224 T4558 T4550 punct ", ",were
R1225 T4559 T4560 advmod only,pairs
R1226 T4621 T4622 det a,pseudogene
R1227 T4560 T4550 nsubj pairs,were
R1228 T4622 T4619 dobj pseudogene,represents
R1229 T4623 T4622 compound duplication,pseudogene
R1230 T4624 T4619 punct .,represents
R1231 T4561 T4560 nummod five,pairs
R1232 T4626 T4627 nsubj Bierbaum,reported
R1233 T4562 T4560 compound base,pairs
R1234 T4628 T4627 advmod previously,reported
R1235 T4629 T4630 det the,existence
R1236 T4563 T4560 prep of,pairs
R1237 T4630 T4627 dobj existence,reported
R1238 T4631 T4630 prep of,existence
R1239 T4632 T4633 nummod two,pseudogenes
R1240 T4564 T4565 det the,region
R1241 T4633 T4631 pobj pseudogenes,of
R1242 T4565 T4563 pobj region,of
R1243 T4634 T4633 prep with,pseudogenes
R1244 T4566 T4565 nummod 5,region
R1245 T4635 T4636 amod similar,organization
R1246 T4567 T4566 punct ',5
R1247 T4636 T4634 pobj organization,with
R1248 T4637 T4638 compound exon,intron
R1249 T4568 T4565 compound flanking,region
R1250 T4638 T4636 compound intron,organization
R1251 T4639 T4638 punct -,intron
R1252 T4640 T4627 prep as,reported
R1253 T4569 T4550 acomp identical,were
R1254 T4641 T4642 det the,gene
R1255 T4642 T4640 pobj gene,as
R1256 T4643 T4642 compound ESG1,gene
R1257 T4570 T4550 punct .,were
R1258 T4644 T4645 punct [,17
R1259 T4645 T4627 parataxis 17,reported
R1260 T4646 T4645 punct ],17
R1261 T4647 T4627 punct .,reported
R1262 T4572 T4573 det This,region
R1263 T4649 T4650 nsubj We,determine
R1264 T4651 T4650 aux could,determine
R1265 T4573 T4577 nsubj region,possess
R1266 T4652 T4650 neg not,determine
R1267 T4653 T4654 dep which,corresponds
R1268 T4654 T4650 ccomp corresponds,determine
R1269 T4574 T4573 nummod 5,region
R1270 T4655 T4653 prep of,which
R1271 T4656 T4657 det these,pseudogenes
R1272 T4575 T4574 punct ',5
R1273 T4657 T4655 pobj pseudogenes,of
R1274 T4658 T4657 nummod two,pseudogenes
R1275 T4576 T4573 compound flanking,region
R1276 T4659 T4654 prep to,corresponds
R1277 T4660 T4661 det the,one
R1278 T4661 T4659 pobj one,to
R1279 T4578 T4579 punct (,kbp
R1280 T4662 T4663 nsubj we,identified
R1281 T4663 T4661 advcl identified,one
R1282 T4664 T4661 cc or,one
R1283 T4665 T4666 det the,location
R1284 T4579 T4573 parataxis kbp,region
R1285 T4666 T4661 conj location,one
R1286 T4667 T4666 prep of,location
R1287 T4668 T4669 det the,pseudogene
R1288 T4580 T4581 punct ~,6
R1289 T4669 T4667 pobj pseudogene,of
R1290 T4670 T4669 amod remaining,pseudogene
R1291 T4671 T4650 punct .,determine
R1292 T4581 T4579 nummod 6,kbp
R1293 T4582 T4579 punct ),kbp
R1294 T4583 T4577 aux did,possess
R1295 T4584 T4577 neg not,possess
R1296 T4585 T4586 det any,activity
R1297 T4586 T4577 dobj activity,possess
R1298 T4587 T4588 compound promoter,enhancer
R1299 T4588 T4586 compound enhancer,activity
R1300 T4589 T4588 punct /,enhancer
R1301 T4590 T4577 prep in,possess
R1302 T4591 T4592 compound luciferase,reporter
R1303 T4592 T4593 compound reporter,assays
R1304 T4593 T4590 pobj assays,in
R1305 T4594 T4595 punct (,Figure
R1306 T4595 T4577 parataxis Figure,possess
R1307 T4596 T4595 nummod 3,Figure
R1308 T4597 T4595 punct ),Figure
R1309 T4598 T4577 punct .,possess
R1310 T4600 T4601 nsubj It,is
R1311 T4602 T4601 advmod thus,is
R1312 T4603 T4601 acomp unlikely,is
R1313 T4604 T4605 mark that,transcribed
R1314 T4605 T4601 ccomp transcribed,is
R1315 T4606 T4607 det this,sequence
R1316 T4607 T4605 nsubjpass sequence,transcribed
R1317 T4608 T4605 auxpass is,transcribed
R1318 T4609 T4605 cc or,transcribed
R1319 T4610 T4605 conj translated,transcribed
R1320 T4611 T4610 prep into,translated
R1321 T4612 T4613 det a,protein
R1322 T4613 T4611 pobj protein,into
R1323 T4614 T4613 amod functional,protein
R1324 T4615 T4601 punct .,is
R1325 T4617 T4618 det This,sequence
R1326 T4618 T4619 nsubj sequence,represents
R1327 T4620 T4619 advmod likely,represents
R1329 T6437 T6438 amod Targeted,disruption
R1330 T6439 T6438 prep of,disruption
R1331 T6440 T6441 det the,gene
R1332 T6441 T6439 pobj gene,of
R1333 T6442 T6441 compound mouse,gene
R1334 T6443 T6441 compound ESG1,gene
R1335 T6445 T6446 aux To,study
R1336 T6446 T6447 advcl study,deleted
R1337 T6448 T6449 det the,function
R1338 T6449 T6446 dobj function,study
R1339 T6450 T6449 prep of,function
R1340 T6451 T6450 pobj ESG1,of
R1341 T6452 T6447 punct ", ",deleted
R1342 T6453 T6447 nsubj we,deleted
R1343 T6454 T6455 det the,gene
R1344 T6455 T6447 dobj gene,deleted
R1345 T6456 T6447 prep by,deleted
R1346 T6457 T6458 amod homologous,recombination
R1347 T6458 T6456 pobj recombination,by
R1348 T6459 T6458 prep in,recombination
R1349 T6460 T6461 compound mouse,cells
R1350 T6461 T6459 pobj cells,in
R1351 T6462 T6461 compound ES,cells
R1352 T6463 T6447 punct .,deleted
R1353 T6465 T6466 nsubj We,replaced
R1354 T6467 T6468 det the,exons
R1355 T6468 T6466 dobj exons,replaced
R1356 T6469 T6468 nummod three,exons
R1357 T6470 T6466 prep with,replaced
R1358 T6471 T6472 preconj either,fusion
R1359 T6472 T6470 pobj fusion,with
R1360 T6473 T6472 det a,fusion
R1361 T6474 T6472 prep of,fusion
R1362 T6475 T6476 det the,genes
R1363 T6476 T6474 pobj genes,of
R1364 T6477 T6478 nmod neomycin,resistance
R1365 T6478 T6476 nmod resistance,genes
R1366 T6479 T6478 punct -,resistance
R1367 T6480 T6478 cc and,resistance
R1368 T6481 T6482 compound β,galactosidase
R1369 T6482 T6478 conj galactosidase,resistance
R1370 T6483 T6482 punct -,galactosidase
R1371 T6484 T6485 punct (,geo
R1372 T6485 T6476 parataxis geo,genes
R1373 T6486 T6485 compound β,geo
R1374 T6487 T6485 punct -,geo
R1386 T6499 T6497 dobj vectors,using
R1387 T6500 T6499 compound targeting,vectors
R1388 T6501 T6502 punct (,4A
R1389 T6502 T6499 parataxis 4A,vectors
R1390 T6503 T6502 compound Figure,4A
R1391 T6504 T6502 punct ),4A
R1392 T6505 T6499 acl introduced,vectors
R1393 T6506 T6505 prep into,introduced
R1394 T6507 T6508 compound RF8,cells
R1395 T6508 T6506 pobj cells,into
R1396 T6509 T6508 compound ES,cells
R1397 T6510 T6505 prep by,introduced
R1398 T6511 T6510 pobj electroporation,by
R1399 T6512 T6466 punct .,replaced
R1400 T6514 T6515 nsubj We,obtained
R1401 T6516 T6517 nummod eight,clones
R1402 T6517 T6515 dobj clones,obtained
R1403 T6518 T6517 compound ES,clones
R1404 T6519 T6517 compound cell,clones
R1405 T6520 T6515 prep with,obtained
R1406 T6521 T6522 amod correct,recombination
R1407 T6522 T6520 pobj recombination,with
R1408 T6523 T6522 amod homologous,recombination
R1409 T6524 T6522 prep of,recombination
R1410 T6525 T6526 det the,vector
R1411 T6526 T6524 pobj vector,of
R1412 T6527 T6528 compound β,geo
R1413 T6528 T6526 compound geo,vector
R1414 T6529 T6528 punct -,geo
R1415 T6530 T6526 compound targeting,vector
R1416 T6531 T6522 punct ", ",recombination
R1417 T6532 T6533 dep which,confirmed
R1418 T6533 T6522 relcl confirmed,recombination
R1419 T6534 T6533 auxpass was,confirmed
R1420 T6535 T6533 agent by,confirmed
R1421 T6536 T6537 compound Southern,blot
R1422 T6537 T6538 compound blot,analysis
R1423 T6538 T6535 pobj analysis,by
R1424 T6539 T6540 punct (,4B
R1425 T6540 T6533 parataxis 4B,confirmed
R1426 T6541 T6540 compound Figure,4B
R1427 T6542 T6540 punct ),4B
R1428 T6543 T6515 punct .,obtained
R1429 T6545 T6546 nsubj We,obtained
R1430 T6547 T6548 advmod only,clone
R1431 T6548 T6546 dobj clone,obtained
R1432 T6549 T6548 nummod one,clone
R1433 T6550 T6548 prep with,clone
R1434 T6551 T6552 amod correct,recombination
R1435 T6552 T6550 pobj recombination,with
R1436 T6553 T6552 amod homologous,recombination
R1437 T6554 T6552 prep of,recombination
R1438 T6555 T6556 det the,vector
R1439 T6556 T6554 pobj vector,of
R1440 T6557 T6556 compound HygR,vector
R1441 T6558 T6556 compound targeting,vector
R1442 T6559 T6546 punct .,obtained
R1443 T6562 T6563 amod T,o
R1444 T6564 T6563 prep o,o
R1469 T6613 T6604 acomp homozygous,were
R1470 T6614 T6613 prep for,homozygous
R1471 T6615 T6616 compound ESG1,deletion
R1472 T6616 T6614 pobj deletion,for
R1473 T6617 T6604 punct .,were
R1474 T6619 T6620 nmod Northern,blot
R1475 T6620 T6621 nmod blot,analyses
R1476 T6621 T6625 nsubj analyses,confirmed
R1477 T6622 T6620 cc and,blot
R1478 T6623 T6624 compound western,blot
R1479 T6624 T6620 conj blot,blot
R1480 T6626 T6627 det the,absence
R1481 T6627 T6625 dobj absence,confirmed
R1482 T6628 T6627 prep of,absence
R1483 T6629 T6628 pobj ESG1,of
R1484 T6630 T6627 prep in,absence
R1485 T6631 T6632 det these,cells
R1486 T6632 T6630 pobj cells,in
R1487 T6633 T6634 punct (,4C
R1488 T6634 T6625 parataxis 4C,confirmed
R1489 T6635 T6634 compound Figure,4C
R1490 T6636 T6634 punct ),4C
R1491 T6637 T6625 punct .,confirmed
R1492 T6639 T6640 prep In,replaced
R1493 T6641 T6642 nummod 29,clones
R1494 T6642 T6639 pobj clones,In
R1495 T6643 T6640 punct ", ",replaced
R1496 T6644 T6645 det the,vector
R1497 T6645 T6640 nsubj vector,replaced
R1498 T6646 T6647 compound β,geo
R1499 T6647 T6645 compound geo,vector
R1500 T6648 T6647 punct -,geo
R1501 T6649 T6640 aux had,replaced
R1502 T6650 T6651 det the,vector
R1503 T6651 T6640 dobj vector,replaced
R1504 T6652 T6651 compound HygR,vector
R1505 T6653 T6640 punct ", ",replaced
R1506 T6654 T6655 amod such,remained
R1507 T6655 T6640 advcl remained,replaced
R1508 T6656 T6655 mark that,remained
R1509 T6657 T6658 det the,cells
R1510 T6658 T6655 nsubj cells,remained
R1511 T6659 T6655 acomp heterozygous,remained
R1512 T6660 T6640 punct .,replaced
R1513 T6662 T6663 prep In,integrated
R1514 T6664 T6665 det the,clones
R1515 T6665 T6662 pobj clones,In
R1516 T6666 T6665 amod remaining,clones
R1517 T6667 T6665 nummod 27,clones
R1518 T6668 T6663 punct ", ",integrated
R1519 T6669 T6670 det the,vector
R1520 T6670 T6663 nsubjpass vector,integrated
R1521 T6671 T6672 compound β,geo
R1522 T6672 T6670 compound geo,vector
R1523 T6673 T6672 punct -,geo
R1524 T6674 T6663 auxpass was,integrated
R1525 T6675 T6663 prep at,integrated
R1526 T6676 T6677 amod non-homologous,sites
R1527 T6677 T6675 pobj sites,at
R1528 T6678 T6663 punct .,integrated
R1529 T6680 T6681 nmod ESG1,cells
R1530 T6681 T6686 nsubj cells,exhibited
R1531 T6682 T6680 punct -,ESG1
R1532 T6683 T6680 punct /,ESG1
R1533 T6684 T6680 punct -,ESG1
R1534 T6685 T6681 compound ES,cells
R1535 T6687 T6688 amod normal,morphology
R1536 T6688 T6686 dobj morphology,exhibited
R1537 T6689 T6690 punct (,5A
R1538 T6690 T6686 parataxis 5A,exhibited
R1539 T6691 T6690 compound Figure,5A
R1540 T6692 T6690 punct ),5A
R1541 T6693 T6686 punct .,exhibited
R1542 T6695 T6696 det These,cells
R1543 T6696 T6697 nsubj cells,proliferated
R1544 T6698 T6697 advmod also,proliferated
R1545 T6699 T6697 prep at,proliferated
R1546 T6700 T6701 det a,speed
R1547 T6701 T6699 pobj speed,at
R1548 T6702 T6701 amod comparable,speed
R1549 T6703 T6702 prep to,comparable
R1550 T6704 T6703 pobj that,to
R1551 T6705 T6704 prep of,that
R1552 T6706 T6707 det the,cells
R1553 T6707 T6705 pobj cells,of
R1554 T6708 T6707 nmod control,cells
R1555 T6709 T6710 punct (,heterozygous
R1556 T6710 T6708 parataxis heterozygous,control
R1557 T6711 T6710 cc and,heterozygous
R1558 T6712 T6713 amod wild,type
R1559 T6713 T6710 conj type,heterozygous
R1560 T6714 T6713 punct -,type
R1561 T6715 T6710 punct ),heterozygous
R1562 T6716 T6717 punct (,5B
R1563 T6717 T6697 parataxis 5B,proliferated
R1564 T6718 T6717 compound Figure,5B
R1565 T6719 T6717 punct ),5B
R1566 T6720 T6697 punct .,proliferated
R1567 T6722 T6723 nmod ESG1,cells
R1568 T6723 T6727 nsubj cells,differentiated
R1569 T6724 T6722 punct -,ESG1
R1570 T6725 T6722 punct /,ESG1
R1571 T6726 T6722 punct -,ESG1
R1572 T6728 T6727 advmod normally,differentiated
R1573 T6729 T6727 prep after,differentiated
R1574 T6730 T6731 det the,removal
R1575 T6731 T6729 pobj removal,after
R1576 T6732 T6731 prep of,removal
R1577 T6733 T6734 nmod leukemia,factor
R1578 T6734 T6732 pobj factor,of
R1579 T6735 T6734 amod inhibitory,factor
R1580 T6736 T6737 punct (,5B
R1581 T6737 T6734 parataxis 5B,factor
R1582 T6738 T6737 compound Figure,5B
R1583 T6739 T6737 punct ),5B
R1584 T6740 T6731 cc or,removal
R1614 T6772 T6773 punct (,5C
R1615 T6773 T6752 parataxis 5C,produced
R1616 T6774 T6773 compound Figure,5C
R1617 T6775 T6773 punct ),5C
R1618 T6776 T6752 punct .,produced
R1619 T6778 T6779 det These,results
R1620 T6779 T6780 nsubj results,indicate
R1621 T6781 T6782 mark that,is
R1622 T6782 T6780 ccomp is,indicate
R1623 T6783 T6782 nsubj ESG1,is
R1624 T6784 T6782 acomp dispensable,is
R1625 T6785 T6784 prep for,dispensable
R1626 T6786 T6787 det the,properties
R1627 T6787 T6785 pobj properties,for
R1628 T6788 T6789 compound self,renewal
R1629 T6789 T6787 compound renewal,properties
R1630 T6790 T6789 punct -,renewal
R1631 T6791 T6787 cc and,properties
R1632 T6792 T6787 conj pluripotency,properties
R1633 T6793 T6792 prep of,pluripotency
R1634 T6794 T6795 compound ES,cells
R1635 T6795 T6793 pobj cells,of
R1636 T6796 T6780 punct .,indicate
R1653 T6822 T6818 dobj microarrays,using
R1654 T6823 T6822 compound DNA,microarrays
R1655 T6824 T6822 acl representing,microarrays
R1656 T6825 T6826 punct ~,"20,000"
R1657 T6826 T6827 nummod "20,000",genes
R1658 T6827 T6824 dobj genes,representing
R1659 T6828 T6806 punct .,mined
R1660 T6830 T6831 prep In,identified
R1661 T6832 T6830 pobj comparison,In
R1662 T6833 T6832 prep to,comparison
R1663 T6834 T6835 compound control,cells
R1664 T6835 T6833 pobj cells,to
R1665 T6836 T6835 compound ES,cells
R1666 T6837 T6831 punct ", ",identified
R1667 T6838 T6831 nsubjpass ESG1,identified
R1668 T6839 T6831 auxpass was,identified
R1669 T6840 T6831 prep as,identified
R1670 T6841 T6842 det the,gene
R1671 T6842 T6840 pobj gene,as
R1672 T6843 T6842 acl reduced,gene
R1673 T6844 T6843 prep to,reduced
R1674 T6845 T6846 det the,extent
R1675 T6846 T6844 pobj extent,to
R1676 T6847 T6846 amod greatest,extent
R1677 T6848 T6843 prep in,reduced
R1678 T6849 T6850 nmod ESG1,cells
R1679 T6850 T6848 pobj cells,in
R1680 T6851 T6849 punct -,ESG1
R1681 T6852 T6849 punct /,ESG1
R1682 T6853 T6849 punct -,ESG1
R1683 T6854 T6850 compound ES,cells
R1684 T6855 T6856 punct (,6A
R1685 T6856 T6842 parataxis 6A,gene
R1686 T6857 T6856 compound Figure,6A
R1687 T6858 T6856 punct ),6A
R1688 T6859 T6831 punct .,identified
R1689 T6861 T6862 det The,expression
R1690 T6862 T6863 nsubj expression,was
R1691 T6864 T6862 prep of,expression
R1692 T6865 T6866 compound ES,cell
R1693 T6866 T6867 compound cell,genes
R1694 T6867 T6864 pobj genes,of
R1695 T6868 T6867 compound marker,genes
R1696 T6869 T6867 punct ", ",genes
R1697 T6870 T6871 amod such,as
R1698 T6871 T6867 prep as,genes
R1699 T6872 T6871 pobj Nanog,as
R1700 T6873 T6872 cc and,Nanog
R1701 T6874 T6872 conj Oct3,Nanog
R1702 T6875 T6874 punct /,Oct3
R1703 T6876 T6874 nummod 4,Oct3
R1704 T6877 T6863 punct ", ",was
R1705 T6878 T6863 acomp normal,was
R1706 T6879 T6863 prep in,was
R1707 T6880 T6881 nmod ESG1,cells
R1708 T6881 T6879 pobj cells,in
R1709 T6882 T6880 punct -,ESG1
R1710 T6883 T6880 punct /,ESG1
R1711 T6884 T6880 punct -,ESG1
R1712 T6885 T6881 compound ES,cells
R1713 T6886 T6863 punct .,was
R1714 T6888 T6889 nsubj We,confirmed
R1715 T6890 T6891 amod normal,expression
R1716 T6891 T6889 dobj expression,confirmed
R1717 T6892 T6891 nmod Oct3,expression
R1718 T6893 T6892 punct /,Oct3
R1719 T6894 T6892 nummod 4,Oct3
R1720 T6895 T6891 prep at,expression
R1721 T6896 T6897 compound protein,levels
R1722 T6897 T6895 pobj levels,at
R1723 T6898 T6889 prep by,confirmed
R1724 T6899 T6900 compound Western,blot
R1725 T6900 T6898 pobj blot,by
R1726 T6901 T6902 punct (,6B
R1727 T6902 T6889 parataxis 6B,confirmed
R1728 T6903 T6902 compound Figure,6B
R1729 T6904 T6902 punct ),6B
R1730 T6905 T6889 punct .,confirmed
R1731 T6907 T6908 det The,profiles
R1732 T6908 T6912 nsubj profiles,were
R1733 T6909 T6908 amod overall,profiles
R1734 T6910 T6911 compound gene,expression
R1735 T6911 T6908 compound expression,profiles
R1736 T6913 T6912 acomp similar,were
R1737 T6914 T6912 prep between,were
R1738 T6915 T6916 compound control,cells
R1739 T6916 T6914 pobj cells,between
R1740 T6917 T6916 compound ES,cells
R1741 T6918 T6916 cc and,cells
R1742 T6919 T6920 nmod ESG1,cells
R1743 T6920 T6916 conj cells,cells
R1744 T6921 T6919 punct -,ESG1
R1745 T6922 T6919 punct /,ESG1
R1746 T6923 T6919 punct -,ESG1
R1747 T6924 T6920 compound ES,cells
R1748 T6925 T6912 punct .,were
R1749 T6927 T6928 amod Several,genes
R1750 T6928 T6929 nsubj genes,exhibited
R1751 T6930 T6931 det a,reduction
R1752 T6931 T6929 dobj reduction,exhibited
R1753 T6932 T6933 amod greater,two-fold
R1754 T6933 T6931 nummod two-fold,reduction
R1755 T6934 T6933 quantmod than,two-fold
R1756 T6935 T6929 prep in,exhibited
R1757 T6936 T6937 nmod ESG1,cells
R1758 T6937 T6935 pobj cells,in
R1759 T6938 T6936 punct -,ESG1
R1760 T6939 T6936 punct /,ESG1
R1761 T6940 T6936 punct -,ESG1
R1762 T6941 T6929 punct ", ",exhibited
R1763 T6942 T6929 prep including,exhibited
R1764 T6943 T6942 pobj Krt1,including
R1765 T6944 T6943 punct -,Krt1
R1766 T6945 T6943 nummod 8,Krt1
R1767 T6946 T6943 punct ", ",Krt1
R1768 T6947 T6943 conj Pem,Krt1
R1769 T6948 T6947 punct ", ",Pem
R1770 T6949 T6947 conj Ctgf,Pem
R1771 T6950 T6949 punct ", ",Ctgf
R1772 T6951 T6949 conj Ptgs2,Ctgf
R1773 T6952 T6951 punct ", ",Ptgs2
R1774 T6953 T6951 conj Igf2,Ptgs2
R1775 T6954 T6953 cc and,Igf2
R1776 T6955 T6953 conj Inhba,Igf2
R1777 T6956 T6929 punct .,exhibited
R1778 T6958 T6959 det These,genes
R1779 T6959 T6960 nsubjpass genes,regulated
R1780 T6961 T6960 aux might,regulated
R1781 T6962 T6960 auxpass be,regulated
R1782 T6963 T6960 advmod directly,regulated
R1857 T7059 T7060 nummod 36,type
R1858 T7060 T7056 appos type,ratios
R1859 T7061 T7060 amod wild,type
R1860 T7062 T7060 punct -,type
R1861 T7063 T7060 punct ", ",type
R1862 T7064 T7065 nummod 69,ESG1
R1863 T7065 T7060 conj ESG1,type
R1864 T7066 T7065 punct +,ESG1
R1865 T7067 T7065 punct /,ESG1
R1866 T7068 T7065 punct -,ESG1
R1867 T7069 T7065 punct ", ",ESG1
R1868 T7070 T7065 cc and,ESG1
R1869 T7071 T7072 nummod 45,ESG1
R1870 T7072 T7065 conj ESG1,ESG1
R1871 T7073 T7072 punct -,ESG1
R1872 T7074 T7072 punct /,ESG1
R1873 T7075 T7072 punct -,ESG1
R1874 T7076 T7056 punct ),ratios
R1875 T7077 T7048 prep from,obtained
R1876 T7078 T7077 pobj intercrosses,from
R1877 T7079 T7078 prep of,intercrosses
R1878 T7080 T7081 nmod ESG1,mice
R1879 T7081 T7079 pobj mice,of
R1880 T7082 T7080 punct +,ESG1
R1881 T7083 T7080 punct /,ESG1
R1882 T7084 T7080 punct -,ESG1
R1883 T7085 T7048 punct .,obtained
R1884 T7087 T7088 det These,animals
R1885 T7088 T7089 nsubj animals,exhibited
R1886 T7090 T7091 amod normal,development
R1887 T7091 T7089 dobj development,exhibited
R1888 T7092 T7091 punct ", ",development
R1889 T7093 T7094 amod gross,appearance
R1890 T7094 T7091 conj appearance,development
R1891 T7095 T7094 punct ", ",appearance
R1892 T7096 T7094 cc and,appearance
R1893 T7097 T7094 conj fertility,appearance
R1894 T7098 T7099 punct (,shown
R1895 T7099 T7089 parataxis shown,exhibited
R1896 T7100 T7099 neg not,shown
R1897 T7121 T7122 det These,data
R1898 T7101 T7099 punct ),shown
R1899 T7122 T7123 nsubj data,demonstrated
R1900 T7102 T7089 punct .,exhibited
R1901 T7124 T7125 mark that,is
R1902 T7125 T7123 ccomp is,demonstrated
R1903 T7126 T7125 nsubj ESG1,is
R1904 T7127 T7125 acomp dispensable,is
R1905 T7128 T7127 prep for,dispensable
R1906 T7129 T7130 preconj both,development
R1907 T7130 T7128 pobj development,for
R1908 T7131 T7130 compound mouse,development
R1909 T7104 T7105 amod Histological,examination
R1910 T7132 T7130 cc and,development
R1911 T7133 T7134 compound germ,cell
R1912 T7134 T7135 compound cell,formation
R1913 T7135 T7130 conj formation,development
R1914 T7136 T7123 punct .,demonstrated
R1915 T7138 T7139 nsubj We,generated
R1916 T7105 T7106 nsubj examination,identify
R1917 T7140 T7139 advmod also,generated
R1918 T7141 T7142 compound ES,cells
R1919 T7142 T7139 dobj cells,generated
R1920 T7107 T7105 prep of,examination
R1921 T7143 T7139 prep from,generated
R1922 T7144 T7143 pobj blastocysts,from
R1923 T7145 T7144 acl obtained,blastocysts
R1924 T7146 T7145 prep by,obtained
R1925 T7108 T7107 pobj testis,of
R1926 T7147 T7146 pobj intercrosses,by
R1927 T7148 T7147 prep of,intercrosses
R1928 T7109 T7108 cc and,testis
R1929 T7149 T7150 nmod ESG1,males
R1930 T7110 T7108 conj ovary,testis
R1931 T7150 T7148 pobj males,of
R1932 T7151 T7149 punct +,ESG1
R1933 T7152 T7149 punct /,ESG1
R1934 T7153 T7149 punct -,ESG1
R1935 T7111 T7106 aux could,identify
R1936 T7154 T7150 cc and,males
R1937 T7155 T7156 nmod ESG1,females
R1938 T7112 T7106 neg not,identify
R1939 T7156 T7150 conj females,males
R1940 T7157 T7155 punct -,ESG1
R1941 T7158 T7155 punct /,ESG1
R1942 T7159 T7155 punct -,ESG1
R1943 T7113 T7114 det any,abnormalities
R1944 T7160 T7139 punct .,generated
R1945 T7162 T7163 prep Of,were
R1946 T7114 T7106 dobj abnormalities,identify
R1947 T7164 T7165 det the,lines
R1948 T7165 T7162 pobj lines,Of
R1949 T7166 T7165 nummod eight,lines
R1950 T7115 T7116 punct (,shown
R1951 T7167 T7165 compound ES,lines
R1952 T7168 T7165 compound cell,lines
R1953 T7169 T7165 acl established,lines
R1954 T7116 T7106 parataxis shown,identify
R1955 T7170 T7163 punct ", ",were
R1956 T7171 T7172 nummod two,clones
R1957 T7172 T7163 nsubj clones,were
R1958 T7173 T7163 attr ESG1,were
R1959 T7174 T7173 punct -,ESG1
R1960 T7175 T7173 punct /,ESG1
R1961 T7176 T7173 punct -,ESG1
R1962 T7177 T7163 punct .,were
R1963 T7117 T7116 neg not,shown
R1964 T7179 T7180 det These,cells
R1965 T7180 T7185 nsubj cells,demonstrated
R1966 T7181 T7182 npadvmod ESG1,null
R1967 T7118 T7116 punct ),shown
R1968 T7182 T7180 amod null,cells
R1969 T7183 T7182 punct -,null
R1970 T7184 T7180 compound ES,cells
R1971 T7119 T7106 punct .,identify
R1972 T7186 T7187 amod normal,morphology
R1973 T7187 T7185 dobj morphology,demonstrated
R1974 T7188 T7187 punct ", ",morphology
R1975 T7189 T7187 conj proliferation,morphology
R1976 T7190 T7189 punct ", ",proliferation
R1977 T7191 T7189 cc and,proliferation
R1978 T7192 T7189 conj differentiation,proliferation
R1979 T7193 T7194 punct (,shown
R1980 T7194 T7192 parataxis shown,differentiation
R1981 T7195 T7194 neg not,shown
R1982 T7196 T7194 punct ),shown
R1983 T7197 T7185 punct ", ",demonstrated
R1984 T7198 T7185 advcl confirming,demonstrated
R1985 T7199 T7200 mark that,is
R1986 T7200 T7198 ccomp is,confirming
R1987 T7201 T7200 nsubj ESG1,is
R1988 T7202 T7200 acomp dispensable,is
R1989 T7203 T7200 prep in,is
R1990 T7204 T7205 compound ES,cells
R1991 T7205 T7203 pobj cells,in
R1992 T7206 T7185 punct .,demonstrated
R1993 T7369 T7370 aux To,analyze
R1994 T7370 T7371 advcl analyze,identified
R1995 T7372 T7373 det the,roles
R1996 T7373 T7370 dobj roles,analyze
R1997 T7374 T7373 amod physiological,roles
R1998 T7375 T7373 prep of,roles
R1999 T7376 T7375 pobj ESG1,of
R2000 T7377 T7371 punct ", ",identified
R2001 T7378 T7371 nsubj we,identified
R2002 T7379 T7380 det the,gene
R2003 T7380 T7371 dobj gene,identified
R2004 T7381 T7380 compound mouse,gene
R2005 T7382 T7371 prep on,identified
R2006 T7383 T7382 pobj chromosome,on
R2007 T7384 T7383 nummod 9,chromosome
R2008 T7385 T7371 cc and,identified
R2009 T7386 T7371 conj deleted,identified
R2010 T7387 T7386 dobj it,deleted
R2011 T7388 T7386 prep by,deleted
R2012 T7389 T7390 amod homologous,recombination
R2013 T7390 T7388 pobj recombination,by
R2014 T7391 T7386 prep in,deleted
R2015 T7392 T7393 compound ES,cells
R2016 T7393 T7391 pobj cells,in
R2017 T7394 T7371 punct .,identified
R2018 T7396 T7397 prep Despite,demonstrated
R2019 T7398 T7399 amod specific,expression
R2020 T7399 T7396 pobj expression,Despite
R2021 T7400 T7399 prep in,expression
R2022 T7401 T7402 amod early,embryos
R2023 T7402 T7400 pobj embryos,in
R2024 T7403 T7402 punct ", ",embryos
R2025 T7404 T7405 compound germ,cells
R2026 T7405 T7402 conj cells,embryos
R2027 T7406 T7405 punct ", ",cells
R2028 T7407 T7405 cc and,cells
R2029 T7408 T7409 amod pluripotent,cells
R2030 T7409 T7405 conj cells,cells
R2031 T7410 T7397 punct ", ",demonstrated
R2032 T7411 T7412 poss our,data
R2033 T7412 T7397 nsubj data,demonstrated
R2034 T7413 T7414 mark that,is
R2035 T7414 T7397 ccomp is,demonstrated
R2036 T7415 T7414 nsubj ESG1,is
R2037 T7416 T7414 acomp dispensable,is
R2038 T7417 T7416 prep for,dispensable
R2039 T7418 T7419 compound mouse,development
R2040 T7419 T7417 pobj development,for
R2041 T7420 T7419 punct ", ",development
R2042 T7421 T7422 compound germ,cell
R2043 T7422 T7423 compound cell,formation
R2044 T7423 T7419 conj formation,development
R2045 T7424 T7423 punct ", ",formation
R2046 T7425 T7423 cc and,formation
R2047 T7426 T7427 compound ES,cell
R2048 T7427 T7428 compound cell,renewal
R2049 T7428 T7423 conj renewal,formation
R2050 T7429 T7428 compound self,renewal
R2051 T7430 T7428 punct -,renewal
R2052 T7431 T7397 punct .,demonstrated
R2053 T8265 T8264 cc and,Identification
R2054 T8266 T8264 conj analyses,Identification
R2055 T8267 T8264 prep of,Identification
R2056 T8268 T8269 compound BAC,clones
R2057 T8269 T8267 pobj clones,of
R2058 T8270 T8269 acl containing,clones
R2059 T8271 T8272 det the,gene
R2060 T8272 T8270 dobj gene,containing
R2061 T8273 T8272 compound mouse,gene
R2062 T8274 T8272 compound ESG1,gene
R2063 T8276 T8277 aux To,identify
R2064 T8277 T8278 advcl identify,performed
R2065 T8279 T8280 amod bacterial,chromosome
R2066 T8280 T8282 nmod chromosome,clones
R2067 T8281 T8280 amod artificial,chromosome
R2068 T8282 T8277 dobj clones,identify
R2069 T8283 T8280 punct (,chromosome
R2070 T8284 T8280 appos BAC,chromosome
R2071 T8285 T8282 punct ),clones
R2072 T8286 T8282 acl containing,clones
R2073 T8287 T8288 compound mouse,gene
R2074 T8288 T8286 dobj gene,containing
R2075 T8289 T8288 compound ESG1,gene
R2076 T8290 T8278 punct ", ",performed
R2077 T8291 T8278 nsubj we,performed
R2078 T8292 T8293 npadvmod PCR,based
R2079 T8293 T8295 amod based,screening
R2080 T8294 T8293 punct -,based
R2081 T8295 T8278 dobj screening,performed
R2082 T8296 T8295 prep of,screening
R2083 T8297 T8298 compound mouse,pools
R2084 T8298 T8296 pobj pools,of
R2085 T8299 T8298 compound BAC,pools
R2086 T8300 T8298 compound library,pools
R2087 T8301 T8298 compound DNA,pools
R2088 T8302 T8303 punct (,Genetics
R2089 T8303 T8295 parataxis Genetics,screening
R2090 T8304 T8303 compound Research,Genetics
R2091 T8305 T8303 punct ),Genetics
R2092 T8306 T8278 advcl using,performed
R2093 T8307 T8308 det the,primers
R2094 T8308 T8306 dobj primers,using
R2095 T8309 T8310 nmod pH34,u38
R2096 T8310 T8308 nmod u38,primers
R2097 T8311 T8310 punct -,u38
R2098 T8312 T8313 punct (,3
R2099 T8313 T8310 parataxis 3,u38
R2100 T8314 T8313 dep 5,3
R2101 T8315 T8314 punct ',5
R2102 T8316 T8313 punct -,3
R2103 T8317 T8313 dep GAAGTCTGGTTCCTTGGCAGG,3
R2104 T8318 T8313 punct -,3
R2105 T8319 T8313 punct ',3
R2106 T8320 T8313 punct ),3
R2107 T8321 T8310 cc and,u38
R2108 T8322 T8323 compound pH34,L394
R2109 T8323 T8310 conj L394,u38
R2110 T8324 T8323 punct -,L394
R2111 T8325 T8326 punct (,3
R2112 T8326 T8323 parataxis 3,L394
R2113 T8327 T8326 dep 5,3
R2114 T8328 T8327 punct ',5
R2115 T8329 T8326 punct -,3
R2116 T8330 T8326 dep ACTCGATACACTGGCCTAGC,3
R2117 T8331 T8326 punct -,3
R2118 T8332 T8326 punct ',3
R2119 T8333 T8326 punct ),3
R2120 T8334 T8278 punct .,performed
R2121 T8336 T8337 prep Following,performed
R2122 T8338 T8339 compound restriction,enzyme
R2123 T8339 T8340 compound enzyme,digestion
R2124 T8340 T8336 pobj digestion,Following
R2125 T8341 T8337 punct ", ",performed
R2126 T8342 T8337 nsubj we,performed
R2127 T8343 T8344 compound Southern,blot
R2128 T8344 T8345 compound blot,analyses
R2129 T8345 T8337 dobj analyses,performed
R2130 T8346 T8345 prep of,analyses
R2131 T8347 T8348 compound BAC,clones
R2132 T8348 T8346 pobj clones,of
R2133 T8349 T8350 mark as,described
R2134 T8350 T8337 advcl described,performed
R2135 T8351 T8352 punct [,20
R2136 T8352 T8350 parataxis 20,described
R2137 T8353 T8352 punct ],20
R2138 T8354 T8350 advcl using,described
R2139 T8355 T8356 det the,probes
R2140 T8356 T8354 dobj probes,using
R2141 T8357 T8358 nmod pH34,U258
R2142 T8358 T8356 nmod U258,probes
R2143 T8359 T8358 punct -,U258
R2144 T8360 T8361 punct (,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2145 T8361 T8358 parataxis CTCGAGTGTACAGTCAAGTGGTTGCTGGGA,U258
R2146 T8362 T8361 nummod 5,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2147 T8363 T8362 punct ',5
R2148 T8364 T8361 punct -,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2149 T8365 T8361 punct -,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2150 T8366 T8361 nummod 3,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2151 T8367 T8361 punct ',CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2152 T8368 T8361 punct ),CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2153 T8369 T8358 punct ", ",U258
R2154 T8370 T8371 compound pH34,U65
R2155 T8371 T8358 conj U65,U258
R2156 T8372 T8371 punct -,U65
R2157 T8373 T8374 punct (,GTGACCCTCGTGACCCGTAA
R2158 T8374 T8371 parataxis GTGACCCTCGTGACCCGTAA,U65
R2159 T8375 T8374 nummod 5,GTGACCCTCGTGACCCGTAA
R2160 T8376 T8375 punct ',5
R2161 T8377 T8374 punct -,GTGACCCTCGTGACCCGTAA
R2162 T8378 T8374 punct -,GTGACCCTCGTGACCCGTAA
R2163 T8379 T8374 nummod 3,GTGACCCTCGTGACCCGTAA
R2164 T8380 T8374 punct ',GTGACCCTCGTGACCCGTAA
R2165 T8381 T8374 punct ),GTGACCCTCGTGACCCGTAA
R2166 T8382 T8371 punct ", ",U65
R2167 T8383 T8384 compound pH34,intron1L
R2168 T8384 T8371 conj intron1L,U65
R2169 T8385 T8384 punct -,intron1L
R2170 T8386 T8387 punct (,CTGCGTGAGAGAAACACCAAACAGGC
R2171 T8387 T8384 parataxis CTGCGTGAGAGAAACACCAAACAGGC,intron1L
R2172 T8388 T8387 nummod 5,CTGCGTGAGAGAAACACCAAACAGGC
R2173 T8389 T8388 punct ',5
R2174 T8390 T8387 punct -,CTGCGTGAGAGAAACACCAAACAGGC
R2175 T8391 T8387 punct -,CTGCGTGAGAGAAACACCAAACAGGC
R2176 T8392 T8387 nummod 3,CTGCGTGAGAGAAACACCAAACAGGC
R2177 T8393 T8387 punct ',CTGCGTGAGAGAAACACCAAACAGGC
R2178 T8394 T8387 punct ),CTGCGTGAGAGAAACACCAAACAGGC
R2179 T8395 T8384 punct ", ",intron1L
R2180 T8396 T8397 compound pH34,L545
R2181 T8397 T8384 conj L545,intron1L
R2182 T8398 T8397 punct -,L545
R2183 T8399 T8400 punct (,TGTGAATGGGAAGGTTACCACTCT
R2184 T8400 T8397 parataxis TGTGAATGGGAAGGTTACCACTCT,L545
R2185 T8401 T8400 nummod 5,TGTGAATGGGAAGGTTACCACTCT
R2186 T8402 T8401 punct ',5
R2187 T8403 T8400 punct -,TGTGAATGGGAAGGTTACCACTCT
R2188 T8404 T8400 punct -,TGTGAATGGGAAGGTTACCACTCT
R2189 T8405 T8400 nummod 3,TGTGAATGGGAAGGTTACCACTCT
R2190 T8406 T8400 punct ',TGTGAATGGGAAGGTTACCACTCT
R2191 T8407 T8400 punct ),TGTGAATGGGAAGGTTACCACTCT
R2192 T8408 T8397 cc and,L545
R2193 T8409 T8410 compound pH34,SCL1
R2194 T8410 T8397 conj SCL1,L545
R2195 T8411 T8410 punct -,SCL1
R2196 T8412 T8413 punct (,GCCCTCTTCTGGTTTGTCTCGAAAT
R2197 T8413 T8410 parataxis GCCCTCTTCTGGTTTGTCTCGAAAT,SCL1
R2198 T8414 T8413 nummod 5,GCCCTCTTCTGGTTTGTCTCGAAAT
R2199 T8415 T8414 punct ',5
R2200 T8416 T8413 punct -,GCCCTCTTCTGGTTTGTCTCGAAAT
R2201 T8417 T8413 punct -,GCCCTCTTCTGGTTTGTCTCGAAAT
R2202 T8418 T8413 nummod 3,GCCCTCTTCTGGTTTGTCTCGAAAT
R2203 T8419 T8413 punct ',GCCCTCTTCTGGTTTGTCTCGAAAT
R2204 T8420 T8413 punct ),GCCCTCTTCTGGTTTGTCTCGAAAT
R2205 T8421 T8337 punct .,performed
R2206 T8423 T8424 nsubj Hybridization,revealed
R2207 T8425 T8423 prep with,Hybridization
R2208 T8426 T8427 det these,probes
R2209 T8427 T8425 pobj probes,with
R2210 T8428 T8424 dobj bands,revealed
R2211 T8429 T8428 acl containing,bands
R2212 T8430 T8431 preconj either,gene
R2213 T8431 T8429 dobj gene,containing
R2214 T8432 T8431 det the,gene
R2215 T8433 T8431 compound ESG1,gene
R2216 T8434 T8431 cc or,gene
R2217 T8435 T8431 conj pseudogenes,gene
R2218 T8436 T8424 punct .,revealed
R2219 T8438 T8439 aux To,sequence
R2220 T8439 T8440 advcl sequence,subcloned
R2221 T8441 T8442 det the,region
R2222 T8442 T8439 dobj region,sequence
R2223 T8443 T8442 acl containing,region
R2224 T8444 T8445 det the,gene
R2225 T8445 T8443 dobj gene,containing
R2226 T8446 T8445 compound mouse,gene
R2227 T8447 T8445 compound ESG1,gene
R2228 T8448 T8445 cc and,gene
R2229 T8449 T8450 det the,region
R2230 T8450 T8445 conj region,gene
R2231 T8451 T8450 nummod 3,region
R2232 T8452 T8451 punct ',3
R2233 T8453 T8450 compound flanking,region
R2234 T8454 T8440 punct ", ",subcloned
R2235 T8455 T8440 nsubj we,subcloned
R2236 T8456 T8457 det a,fragment
R2237 T8457 T8440 dobj fragment,subcloned
R2238 T8458 T8459 punct ~,15
R2239 T8459 T8460 nummod 15,kbp
R2240 T8460 T8457 compound kbp,fragment
R2241 T8461 T8462 compound XhoI,SalI
R2242 T8462 T8457 compound SalI,fragment
R2243 T8463 T8462 punct -,SalI
R2244 T8464 T8440 prep into,subcloned
R2245 T8465 T8466 det the,vector
R2246 T8466 T8464 pobj vector,into
R2247 T8467 T8466 nmod pZERO,vector
R2248 T8468 T8467 punct -,pZERO
R2249 T8469 T8467 nummod 2,pZERO
R2250 T8470 T8471 punct (,Invitrogen
R2251 T8471 T8466 parataxis Invitrogen,vector
R2252 T8472 T8471 punct ),Invitrogen
R2253 T8473 T8440 punct .,subcloned
R2254 T8475 T8476 npadvmod HindIII,digested
R2255 T8476 T8481 amod digested,fragments
R2256 T8477 T8475 punct -,HindIII
R2257 T8478 T8475 cc or,HindIII
R2258 T8479 T8475 conj EcoRI,HindIII
R2259 T8480 T8476 punct -,digested
R2260 T8481 T8482 nsubjpass fragments,cloned
R2261 T8483 T8481 prep of,fragments
R2262 T8484 T8485 det this,vector
R2263 T8485 T8483 pobj vector,of
R2264 T8486 T8482 auxpass were,cloned
R2265 T8487 T8482 advmod then,cloned
R2266 T8488 T8482 prep into,cloned
R2267 T8489 T8490 compound pBluescript,KS
R2268 T8490 T8488 pobj KS,into
R2269 T8491 T8490 punct (,KS
R2270 T8492 T8490 punct -,KS
R2271 T8493 T8490 punct ),KS
R2272 T8494 T8482 prep for,cloned
R2273 T8495 T8494 pobj sequencing,for
R2274 T8496 T8482 punct .,cloned
R2275 T8498 T8499 aux To,sequence
R2276 T8499 T8500 advcl sequence,cloned
R2277 T8501 T8502 det the,pseudogene
R2278 T8502 T8499 dobj pseudogene,sequence
R2279 T8503 T8502 compound ESG1,pseudogene
R2280 T8504 T8502 cc and,pseudogene
R2281 T8505 T8506 det the,region
R2282 T8506 T8502 conj region,pseudogene
R2283 T8507 T8506 nummod 3,region
R2284 T8508 T8507 punct ',3
R2285 T8509 T8506 compound flanking,region
R2286 T8510 T8500 punct ", ",cloned
R2287 T8511 T8512 det an,fragment
R2288 T8512 T8500 nsubjpass fragment,cloned
R2289 T8513 T8514 nummod 8,kbp
R2290 T8514 T8512 compound kbp,fragment
R2291 T8515 T8516 compound NotI,XhoI
R2292 T8516 T8512 compound XhoI,fragment
R2293 T8517 T8516 punct /,XhoI
R2294 T8518 T8500 auxpass was,cloned
R2295 T8519 T8500 prep into,cloned
R2296 T8520 T8521 compound pBluescript,KS
R2297 T8521 T8519 pobj KS,into
R2298 T8522 T8521 punct (,KS
R2299 T8523 T8521 punct -,KS
R2300 T8524 T8521 punct ),KS
R2301 T8525 T8500 punct .,cloned
R2302 T8527 T8528 nmod BamHI,fragments
R2303 T8528 T8533 nsubjpass fragments,cloned
R2304 T8529 T8527 punct -,BamHI
R2305 T8530 T8527 cc or,BamHI
R2306 T8531 T8527 conj PstI,BamHI
R2307 T8532 T8528 punct -,fragments
R2308 T8534 T8528 prep of,fragments
R2309 T8535 T8536 det this,vector
R2310 T8536 T8534 pobj vector,of
R2311 T8537 T8533 auxpass were,cloned
R2312 T8538 T8533 advmod also,cloned
R2313 T8539 T8533 prep into,cloned
R2314 T8540 T8541 compound pBluescript,KS
R2315 T8541 T8539 pobj KS,into
R2316 T8542 T8541 punct (,KS
R2317 T8543 T8541 punct -,KS
R2318 T8544 T8541 punct ),KS
R2319 T8545 T8533 punct .,cloned
R2320 T8547 T8548 aux To,identify
R2321 T8548 T8549 advcl identify,used
R2322 T8550 T8551 det the,sequence
R2323 T8551 T8548 dobj sequence,identify
R2324 T8552 T8551 acl containing,sequence
R2325 T8553 T8554 det the,regions
R2326 T8554 T8552 dobj regions,containing
R2327 T8555 T8554 nummod 5,regions
R2328 T8556 T8555 punct ',5
R2329 T8557 T8554 compound flanking,regions
R2330 T8558 T8554 prep of,regions
R2331 T8559 T8560 det the,gene
R2332 T8560 T8558 pobj gene,of
R2333 T8561 T8560 compound ESG1,gene
R2334 T8562 T8560 cc and,gene
R2335 T8563 T8564 det the,pseudogenes
R2336 T8564 T8560 conj pseudogenes,gene
R2337 T8565 T8564 amod related,pseudogenes
R2338 T8566 T8549 punct ", ",used
R2339 T8567 T8549 nsubj we,used
R2340 T8568 T8569 det a,kit
R2341 T8569 T8549 dobj kit,used
R2342 T8570 T8569 compound TOPO,kit
R2343 T8571 T8569 compound walker,kit
R2344 T8572 T8573 punct (,Invitrogen
R2345 T8573 T8569 parataxis Invitrogen,kit
R2346 T8574 T8573 punct ),Invitrogen
R2347 T8575 T8549 prep with,used
R2348 T8576 T8577 det the,primers
R2349 T8577 T8575 pobj primers,with
R2350 T8578 T8579 nmod pH34,T2L
R2351 T8579 T8577 nmod T2L,primers
R2352 T8580 T8579 punct -,T2L
R2353 T8581 T8582 punct (,3
R2354 T8582 T8579 parataxis 3,T2L
R2355 T8583 T8582 dep 5,3
R2356 T8584 T8583 punct ',5
R2357 T8585 T8582 punct -,3
R2358 T8586 T8582 dep ACTAGTCGCAGCAGGGATCCAGGAATATCT,3
R2359 T8587 T8582 punct -,3
R2360 T8588 T8582 punct ',3
R2361 T8589 T8582 punct ),3
R2362 T8590 T8579 cc and,T2L
R2363 T8591 T8592 compound pH34,L394
R2364 T8592 T8579 conj L394,T2L
R2365 T8593 T8592 punct -,L394
R2366 T8594 T8549 punct .,used
R2367 T8596 T8597 det The,sequence
R2368 T8597 T8599 nsubjpass sequence,cloned
R2369 T8598 T8597 amod resulting,sequence
R2370 T8600 T8599 auxpass was,cloned
R2371 T8601 T8599 prep into,cloned
R2372 T8602 T8601 pobj pCR2.1,into
R2373 T8603 T8604 punct (,Invitrogen
R2374 T8604 T8602 parataxis Invitrogen,pCR2.1
R2375 T8605 T8604 punct ),Invitrogen
R2376 T8606 T8599 punct .,cloned
R2377 T8608 T8609 nsubj We,obtained
R2378 T8609 T8610 ccomp obtained,cloned
R2379 T8611 T8612 det a,band
R2380 T8612 T8609 dobj band,obtained
R2381 T8613 T8614 punct ~,6
R2382 T8614 T8615 nummod 6,kbp
R2383 T8615 T8612 compound kbp,band
R2384 T8616 T8609 prep from,obtained
R2385 T8617 T8618 det the,library
R2386 T8618 T8616 pobj library,from
R2387 T8619 T8620 npadvmod NsiI,digensted
R2388 T8620 T8618 amod digensted,library
R2389 T8621 T8620 punct -,digensted
R2390 T8622 T8610 punct ;,cloned
R2391 T8623 T8624 npadvmod XbaI,digested
R2392 T8624 T8636 amod digested,fragments
R2393 T8625 T8623 punct -,XbaI
R2394 T8626 T8623 punct ", ",XbaI
R2395 T8627 T8623 conj SpeI,XbaI
R2396 T8628 T8627 punct -,SpeI
R2397 T8629 T8627 punct ", ",SpeI
R2398 T8630 T8627 conj EcoRI,SpeI
R2399 T8631 T8630 punct -,EcoRI
R2400 T8632 T8630 punct ", ",EcoRI
R2401 T8633 T8630 cc and,EcoRI
R2402 T8634 T8630 conj PstI,EcoRI
R2403 T8635 T8624 punct -,digested
R2404 T8636 T8610 nsubjpass fragments,cloned
R2405 T8637 T8636 prep of,fragments
R2406 T8638 T8639 det this,band
R2407 T8639 T8637 pobj band,of
R2408 T8640 T8610 auxpass were,cloned
R2409 T8641 T8610 prep into,cloned
R2410 T8642 T8643 compound pBluescript,KS
R2411 T8643 T8641 pobj KS,into
R2412 T8644 T8643 punct (,KS
R2413 T8645 T8643 punct -,KS
R2414 T8646 T8643 punct ),KS
R2415 T8647 T8610 prep for,cloned
R2416 T8648 T8647 pobj sequencing,for
R2417 T8649 T8610 punct .,cloned
R2418 T8651 T8652 det This,fragment
R2419 T8652 T8653 nsubj fragment,contained
R2420 T8654 T8655 det the,region
R2421 T8655 T8653 dobj region,contained
R2422 T8656 T8655 nummod 5,region
R2423 T8657 T8656 punct ',5
R2424 T8658 T8655 compound flanking,region
R2425 T8659 T8655 prep of,region
R2426 T8660 T8661 det the,gene
R2427 T8661 T8659 pobj gene,of
R2428 T8662 T8661 compound ESG1,gene
R2429 T8663 T8653 punct .,contained
R2430 T8665 T8666 det A,fragment
R2431 T8666 T8670 nsubjpass fragment,cloned
R2432 T8667 T8668 punct ~,3
R2433 T8668 T8669 nummod 3,kbp
R2434 T8669 T8666 compound kbp,fragment
R2435 T8671 T8666 punct ", ",fragment
R2436 T8672 T8666 acl obtained,fragment
R2437 T8673 T8672 prep from,obtained
R2438 T8674 T8675 det the,library
R2439 T8675 T8673 pobj library,from
R2440 T8676 T8677 npadvmod SacI,digested
R2441 T8677 T8675 amod digested,library
R2442 T8678 T8677 punct -,digested
R2443 T8679 T8670 punct ", ",cloned
R2444 T8680 T8670 auxpass was,cloned
R2445 T8681 T8670 prep into,cloned
R2446 T8682 T8681 pobj pCR2.1,into
R2447 T8683 T8670 prep for,cloned
R2448 T8684 T8683 pobj sequencing,for
R2449 T8685 T8670 punct .,cloned
R2450 T8687 T8688 det This,fragment
R2451 T8688 T8689 nsubjpass fragment,contained
R2452 T8690 T8689 auxpass was,contained
R2453 T8691 T8692 det the,region
R2454 T8692 T8689 dobj region,contained
R2455 T8693 T8692 nummod 5,region
R2456 T8694 T8693 punct ',5
R2457 T8695 T8692 acl flanking,region
R2458 T8696 T8697 det the,pseudogene
R2459 T8697 T8695 dobj pseudogene,flanking
R2460 T8698 T8689 punct .,contained
R2461 T9172 T9171 prep of,Construction
R2462 T9173 T9174 compound ESG1,vectors
R2463 T9174 T9172 pobj vectors,of
R2464 T9175 T9174 compound targeting,vectors
R2465 T9177 T9178 nsubj We,replaced
R2466 T9179 T9178 dobj all,replaced
R2467 T9180 T9179 prep of,all
R2468 T9181 T9182 det the,exons
R2469 T9182 T9180 pobj exons,of
R2470 T9183 T9182 compound ESG1,exons
R2471 T9184 T9178 prep with,replaced
R2472 T9185 T9186 nummod two,vectors
R2473 T9186 T9184 pobj vectors,with
R2474 T9187 T9186 compound targeting,vectors
R2475 T9188 T9186 acl containing,vectors
R2476 T9189 T9190 preconj either,cassette
R2477 T9190 T9188 dobj cassette,containing
R2478 T9191 T9190 det an,cassette
R2479 T9192 T9193 compound IRES,geo
R2480 T9193 T9190 compound geo,cassette
R2481 T9194 T9193 punct -,geo
R2482 T9195 T9193 compound β,geo
R2483 T9196 T9193 punct -,geo
R2484 T9197 T9198 punct [,21
R2485 T9198 T9190 parataxis 21,cassette
R2486 T9199 T9198 punct ],21
R2487 T9200 T9190 cc or,cassette
R2488 T9201 T9202 det an,HygR
R2489 T9202 T9205 compound HygR,cassette
R2490 T9203 T9202 compound IRES,HygR
R2491 T9204 T9202 punct -,HygR
R2492 T9205 T9190 conj cassette,cassette
R2493 T9206 T9178 prep by,replaced
R2494 T9207 T9208 compound promoter,trap
R2495 T9208 T9209 compound trap,selection
R2496 T9209 T9206 pobj selection,by
R2497 T9210 T9178 punct .,replaced
R2498 T9212 T9213 nsubj We,amplified
R2499 T9214 T9215 det the,arm
R2500 T9215 T9213 dobj arm,amplified
R2501 T9216 T9215 nummod 5,arm
R2502 T9217 T9216 punct ',5
R2503 T9218 T9219 punct (,kbp
R2504 T9219 T9215 parataxis kbp,arm
R2505 T9220 T9219 nummod 1.8,kbp
R2506 T9221 T9219 punct ),kbp
R2507 T9222 T9213 advcl using,amplified
R2508 T9223 T9224 compound KOD,plus
R2509 T9224 T9222 dobj plus,using
R2510 T9225 T9226 punct (,TOYOBO
R2511 T9226 T9224 parataxis TOYOBO,plus
R2512 T9227 T9226 punct ),TOYOBO
R2513 T9228 T9224 prep with,plus
R2514 T9229 T9230 det the,primers
R2515 T9230 T9228 pobj primers,with
R2516 T9231 T9232 nmod pH34,U
R2517 T9232 T9230 nmod U,primers
R2518 T9233 T9232 punct -,U
R2519 T9234 T9232 nmod targetpair5,U
R2520 T9235 T9232 punct -,U
R2521 T9236 T9237 punct (,CCGCGGAAAGTCAAGAGATTGGGTGG
R2522 T9237 T9232 parataxis CCGCGGAAAGTCAAGAGATTGGGTGG,U
R2523 T9238 T9237 nummod 5,CCGCGGAAAGTCAAGAGATTGGGTGG
R2524 T9239 T9238 punct ',5
R2525 T9240 T9237 punct -,CCGCGGAAAGTCAAGAGATTGGGTGG
R2526 T9241 T9237 punct -,CCGCGGAAAGTCAAGAGATTGGGTGG
R2527 T9242 T9237 nummod 3,CCGCGGAAAGTCAAGAGATTGGGTGG
R2528 T9243 T9237 punct ',CCGCGGAAAGTCAAGAGATTGGGTGG
R2529 T9244 T9237 punct ),CCGCGGAAAGTCAAGAGATTGGGTGG
R2530 T9245 T9232 cc and,U
R2531 T9246 T9247 compound pH34,L
R2532 T9247 T9232 conj L,U
R2533 T9248 T9247 punct -,L
R2534 T9249 T9247 compound targetpair5,L
R2535 T9250 T9247 punct -,L
R2536 T9251 T9252 punct (,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2537 T9252 T9247 parataxis GCGGCCGCCTTTACGGGTCACGAGGGTCAC,L
R2538 T9253 T9252 nummod 5,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2539 T9254 T9253 punct ',5
R2540 T9255 T9252 punct -,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2541 T9256 T9252 punct -,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2542 T9257 T9252 nummod 3,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2543 T9258 T9252 punct ',GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2544 T9259 T9252 punct ),GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2545 T9260 T9213 punct .,amplified
R2546 T9262 T9263 det The,arm
R2547 T9263 T9266 nsubjpass arm,amplified
R2548 T9264 T9263 nummod 3,arm
R2549 T9265 T9264 punct ',3
R2550 T9267 T9268 punct (,kbp
R2551 T9268 T9263 parataxis kbp,arm
R2552 T9269 T9268 nummod 5.8,kbp
R2553 T9270 T9268 punct ),kbp
R2554 T9271 T9266 auxpass was,amplified
R2555 T9272 T9266 advcl using,amplified
R2556 T9273 T9274 det the,primers
R2557 T9274 T9272 dobj primers,using
R2558 T9275 T9276 nmod pH34,U
R2559 T9276 T9274 nmod U,primers
R2560 T9277 T9276 punct -,U
R2561 T9278 T9276 nmod targetpair3,U
R2562 T9279 T9276 punct -,U
R2563 T9280 T9281 punct (,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2564 T9281 T9276 parataxis TGTGGCCAGTGTTTGGTTCTGGCGGG,U
R2565 T9282 T9281 nummod 5,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2566 T9283 T9282 punct ',5
R2567 T9284 T9281 punct -,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2568 T9285 T9281 punct -,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2569 T9286 T9281 nummod 3,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2570 T9287 T9281 punct ',TGTGGCCAGTGTTTGGTTCTGGCGGG
R2571 T9288 T9281 punct ),TGTGGCCAGTGTTTGGTTCTGGCGGG
R2572 T9289 T9276 cc and,U
R2573 T9290 T9291 compound pH34,L
R2574 T9291 T9276 conj L,U
R2575 T9292 T9291 punct -,L
R2576 T9293 T9291 compound targetpair3,L
R2577 T9294 T9291 punct -,L
R2578 T9295 T9296 punct (,CTCGAGGACTCGCCATTCTAGCCAAG
R2579 T9296 T9291 parataxis CTCGAGGACTCGCCATTCTAGCCAAG,L
R2580 T9297 T9296 nummod 5,CTCGAGGACTCGCCATTCTAGCCAAG
R2581 T9298 T9297 punct ',5
R2582 T9299 T9296 punct -,CTCGAGGACTCGCCATTCTAGCCAAG
R2583 T9300 T9296 punct -,CTCGAGGACTCGCCATTCTAGCCAAG
R2584 T9301 T9296 nummod 3,CTCGAGGACTCGCCATTCTAGCCAAG
R2585 T9302 T9296 punct ',CTCGAGGACTCGCCATTCTAGCCAAG
R2586 T9303 T9296 punct ),CTCGAGGACTCGCCATTCTAGCCAAG
R2587 T9304 T9266 punct .,amplified
R2588 T9306 T9307 det The,cassettes
R2589 T9307 T9315 nsubjpass cassettes,ligated
R2590 T9308 T9309 nmod IRES,geo
R2591 T9309 T9307 nmod geo,cassettes
R2592 T9310 T9309 nmod β,geo
R2593 T9311 T9309 punct -,geo
R2594 T9312 T9309 cc or,geo
R2595 T9313 T9314 compound IRES,HygR
R2596 T9314 T9309 conj HygR,geo
R2597 T9316 T9315 auxpass were,ligated
R2598 T9317 T9315 prep in,ligated
R2599 T9318 T9317 prep between,in
R2600 T9319 T9320 det the,fragments
R2601 T9320 T9318 pobj fragments,between
R2602 T9321 T9320 nummod two,fragments
R2603 T9322 T9320 compound PCR,fragments
R2604 T9323 T9315 punct .,ligated
R2605 T9325 T9326 det The,cassette
R2606 T9326 T9330 nsubjpass cassette,placed
R2607 T9327 T9328 compound diphtheria,A
R2608 T9328 T9326 compound A,cassette
R2609 T9329 T9328 compound toxin,A
R2610 T9331 T9330 auxpass was,placed
R2611 T9332 T9330 advmod downstream,placed
R2612 T9333 T9332 prep of,downstream
R2613 T9334 T9335 det the,arm
R2614 T9335 T9333 pobj arm,of
R2615 T9336 T9335 nummod 3,arm
R2616 T9337 T9336 punct ',3
R2617 T9338 T9330 punct .,placed
R2618 T9340 T9341 prep After,electroporated
R2619 T9342 T9340 pobj linearization,After
R2620 T9343 T9342 prep with,linearization
R2621 T9344 T9343 pobj SacII,with
R2622 T9345 T9341 punct ", ",electroporated
R2623 T9346 T9347 det these,vectors
R2624 T9347 T9341 nsubjpass vectors,electroporated
R2625 T9348 T9347 compound targeting,vectors
R2626 T9349 T9341 auxpass were,electroporated
R2627 T9350 T9341 prep into,electroporated
R2628 T9351 T9352 quantmod 2.0,107
R2629 T9352 T9354 nummod 107,cells
R2630 T9353 T9352 punct ×,107
R2631 T9354 T9350 pobj cells,into
R2632 T9355 T9354 compound RF8,cells
R2633 T9356 T9354 compound ES,cells
R2634 T9357 T9358 punct [,22
R2635 T9358 T9354 parataxis 22,cells
R2636 T9359 T9358 punct ],22
R2637 T9360 T9341 advcl using,electroporated
R2638 T9361 T9362 det a,pulser
R2639 T9362 T9360 dobj pulser,using
R2640 T9363 T9362 compound Gene,pulser
R2641 T9364 T9365 punct (,BIORAD
R2642 T9365 T9362 parataxis BIORAD,pulser
R2643 T9366 T9365 punct ),BIORAD
R2644 T9367 T9341 punct .,electroporated
R2645 T9369 T9370 amod Transfected,cells
R2646 T9370 T9371 nsubjpass cells,selected
R2647 T9372 T9371 auxpass were,selected
R2648 T9373 T9371 prep with,selected
R2649 T9374 T9375 nummod 250,μg
R2650 T9375 T9376 nmod μg,G418
R2651 T9376 T9373 pobj G418,with
R2652 T9377 T9378 punct /,mL
R2653 T9378 T9375 prep mL,μg
R2654 T9379 T9376 cc or,G418
R2655 T9380 T9381 nummod 100,μg
R2656 T9381 T9382 nmod μg,B
R2657 T9382 T9376 conj B,G418
R2658 T9383 T9384 punct /,mL
R2659 T9384 T9381 prep mL,μg
R2660 T9385 T9382 compound hygromycin,B
R2661 T9386 T9371 punct ", ",selected
R2662 T9387 T9371 advmod respectively,selected
R2663 T9388 T9371 punct .,selected
R2664 T9390 T9391 amod Genomic,DNA
R2665 T9391 T9392 nsubjpass DNA,screened
R2666 T9393 T9391 prep from,DNA
R2667 T9394 T9395 npadvmod G418,resistant
R2668 T9395 T9401 amod resistant,colonies
R2669 T9396 T9394 punct -,G418
R2670 T9397 T9394 cc or,G418
R2671 T9398 T9399 compound hygromycin,B
R2672 T9399 T9394 conj B,G418
R2673 T9400 T9395 punct -,resistant
R2674 T9401 T9393 pobj colonies,from
R2675 T9402 T9392 auxpass was,screened
R2676 T9403 T9392 prep for,screened
R2677 T9404 T9405 amod homologous,recombination
R2678 T9405 T9403 pobj recombination,for
R2679 T9406 T9392 agent by,screened
R2680 T9407 T9408 compound Southern,blotting
R2681 T9408 T9406 pobj blotting,by
R2682 T9409 T9392 punct .,screened
R2683 T9832 T9833 compound Southern,blot
R2684 T9833 T9834 compound blot,screening
R2685 T9835 T9834 prep for,screening
R2686 T9836 T9837 amod homologous,recombination
R2687 T9837 T9835 pobj recombination,for
R2688 T9839 T9840 nmod ES,cells
R2689 T9840 T9841 nmod cells,DNA
R2690 T9841 T9843 nsubjpass DNA,extracted
R2691 T9842 T9841 amod genomic,DNA
R2692 T9844 T9843 auxpass was,extracted
R2693 T9845 T9843 prep with,extracted
R2694 T9846 T9847 nmod PUREGENE,Solution
R2695 T9847 T9845 pobj Solution,with
R2696 T9848 T9846 punct ™,PUREGENE
R2697 T9849 T9850 compound Cell,Lysis
R2698 T9850 T9847 compound Lysis,Solution
R2699 T9851 T9852 punct (,systems
R2700 T9852 T9847 parataxis systems,Solution
R2701 T9853 T9852 compound Gentra,systems
R2702 T9854 T9852 punct ),systems
R2703 T9855 T9843 punct .,extracted
R2704 T9857 T9858 prep For,digested
R2705 T9859 T9860 nummod 5,analysis
R2706 T9860 T9857 pobj analysis,For
R2707 T9861 T9859 punct ',5
R2708 T9862 T9863 compound Southern,blot
R2709 T9863 T9860 compound blot,analysis
R2710 T9864 T9858 punct ", ",digested
R2711 T9865 T9866 amod genomic,DNA
R2712 T9866 T9858 nsubjpass DNA,digested
R2713 T9867 T9858 auxpass was,digested
R2714 T9868 T9858 advmod first,digested
R2715 T9869 T9858 prep with,digested
R2716 T9870 T9869 pobj PstI,with
R2717 T9871 T9858 punct ", ",digested
R2718 T9872 T9873 advmod then,separated
R2719 T9873 T9858 conj separated,digested
R2720 T9874 T9873 prep on,separated
R2721 T9875 T9876 det an,gel
R2722 T9876 T9874 pobj gel,on
R2723 T9877 T9878 nummod 0.8,%
R2724 T9878 T9876 compound %,gel
R2725 T9879 T9876 compound agarose,gel
R2726 T9880 T9873 cc and,separated
R2727 T9881 T9873 conj transferred,separated
R2728 T9882 T9881 prep to,transferred
R2729 T9883 T9884 det a,membrane
R2730 T9884 T9882 pobj membrane,to
R2731 T9885 T9884 compound nylon,membrane
R2732 T9886 T9887 mark as,described
R2733 T9887 T9881 advcl described,transferred
R2734 T9888 T9889 punct [,20
R2735 T9889 T9887 parataxis 20,described
R2736 T9890 T9889 punct ],20
R2737 T9891 T9858 punct .,digested
R2738 T9893 T9894 det A,probe
R2739 T9894 T9899 nsubjpass probe,amplified
R2740 T9895 T9896 nummod 560,bp
R2741 T9896 T9894 nmod bp,probe
R2742 T9897 T9894 nummod 5,probe
R2743 T9898 T9897 punct ',5
R2744 T9900 T9899 auxpass was,amplified
R2745 T9901 T9899 advcl using,amplified
R2746 T9902 T9903 det the,ESG1S5
R2747 T9903 T9901 dobj ESG1S5,using
R2748 T9904 T9905 punct (,GATGGTGGTGGTGACTCAGAG
R2749 T9905 T9903 parataxis GATGGTGGTGGTGACTCAGAG,ESG1S5
R2750 T9906 T9905 nummod 5,GATGGTGGTGGTGACTCAGAG
R2751 T9907 T9906 punct ',5
R2752 T9908 T9905 punct -,GATGGTGGTGGTGACTCAGAG
R2753 T9909 T9905 punct -,GATGGTGGTGGTGACTCAGAG
R2754 T9910 T9905 nummod 3,GATGGTGGTGGTGACTCAGAG
R2755 T9911 T9905 punct ',GATGGTGGTGGTGACTCAGAG
R2756 T9912 T9905 punct ),GATGGTGGTGGTGACTCAGAG
R2757 T9913 T9903 cc and,ESG1S5
R2758 T9914 T9903 conj ESG1AS5,ESG1S5
R2759 T9915 T9901 prep as,using
R2760 T9916 T9917 punct (,primers
R2761 T9917 T9915 pobj primers,as
R2762 T9918 T9919 nummod 5,CCTCCATTGCCTCTATATCAG
R2763 T9919 T9917 nmod CCTCCATTGCCTCTATATCAG,primers
R2764 T9920 T9918 punct ',5
R2765 T9921 T9919 punct -,CCTCCATTGCCTCTATATCAG
R2766 T9922 T9919 punct -,CCTCCATTGCCTCTATATCAG
R2767 T9923 T9919 nummod 3,CCTCCATTGCCTCTATATCAG
R2768 T9924 T9919 punct ',CCTCCATTGCCTCTATATCAG
R2769 T9925 T9919 punct ),CCTCCATTGCCTCTATATCAG
R2770 T9926 T9899 punct .,amplified
R2771 T9928 T9929 det The,probe
R2772 T9929 T9930 nsubj probe,labeled
R2773 T9931 T9930 advmod specifically,labeled
R2774 T9932 T9933 det an,band
R2775 T9933 T9930 dobj band,labeled
R2776 T9934 T9935 nummod 18,kbp
R2777 T9935 T9933 compound kbp,band
R2778 T9936 T9933 prep from,band
R2779 T9937 T9938 det the,locus
R2780 T9938 T9936 pobj locus,from
R2781 T9939 T9938 nmod wild,locus
R2782 T9940 T9939 punct -,wild
R2783 T9941 T9938 compound type,locus
R2784 T9942 T9933 punct ", ",band
R2785 T9943 T9944 det a,band
R2786 T9944 T9933 conj band,band
R2787 T9945 T9946 nummod 15,kbp
R2788 T9946 T9944 compound kbp,band
R2789 T9947 T9944 prep from,band
R2790 T9948 T9949 det the,locus
R2791 T9949 T9947 pobj locus,from
R2792 T9950 T9951 compound β,geo
R2793 T9951 T9949 compound geo,locus
R2794 T9952 T9951 punct -,geo
R2795 T9953 T9944 punct ", ",band
R2796 T9954 T9944 cc and,band
R2797 T9955 T9956 det a,band
R2798 T9956 T9944 conj band,band
R2799 T9957 T9958 nummod 12,kbp
R2800 T9958 T9956 compound kbp,band
R2801 T9959 T9956 prep from,band
R2802 T9960 T9961 det the,locus
R2803 T9961 T9959 pobj locus,from
R2804 T9962 T9961 compound HygR,locus
R2805 T9963 T9930 punct .,labeled
R2806 T9965 T9966 amod Genomic,DNA
R2807 T9966 T9967 nsubjpass DNA,digested
R2808 T9968 T9967 auxpass was,digested
R2809 T9969 T9967 advmod also,digested
R2810 T9970 T9967 prep with,digested
R2811 T9971 T9970 pobj SpeI,with
R2812 T9972 T9967 prep for,digested
R2813 T9973 T9974 nummod 3,analysis
R2814 T9974 T9972 pobj analysis,for
R2815 T9975 T9973 punct ',3
R2816 T9976 T9977 compound Southern,blot
R2817 T9977 T9974 compound blot,analysis
R2818 T9978 T9967 punct .,digested
R2819 T9980 T9981 det A,probe
R2820 T9981 T9986 nsubjpass probe,amplified
R2821 T9982 T9983 nummod "1,010",bp
R2822 T9983 T9981 nmod bp,probe
R2823 T9984 T9981 nummod 3,probe
R2824 T9985 T9984 punct ',3
R2825 T9987 T9986 auxpass was,amplified
R2826 T9988 T9986 prep with,amplified
R2827 T9989 T9990 det the,primers
R2828 T9990 T9988 pobj primers,with
R2829 T9991 T9990 nmod pH34U,primers
R2830 T9992 T9991 punct -,pH34U
R2831 T9993 T9991 nummod 8000,pH34U
R2832 T9994 T9995 punct (,CCAACCAGCCAGAGTTTCAGTTAT
R2833 T9995 T9991 parataxis CCAACCAGCCAGAGTTTCAGTTAT,pH34U
R2834 T9996 T9995 nummod 5,CCAACCAGCCAGAGTTTCAGTTAT
R2835 T9997 T9996 punct ',5
R2836 T9998 T9995 punct -,CCAACCAGCCAGAGTTTCAGTTAT
R2837 T9999 T9995 punct -,CCAACCAGCCAGAGTTTCAGTTAT
R2838 T10000 T9995 nummod 3,CCAACCAGCCAGAGTTTCAGTTAT
R2839 T10001 T9995 punct ',CCAACCAGCCAGAGTTTCAGTTAT
R2840 T10002 T9995 punct ),CCAACCAGCCAGAGTTTCAGTTAT
R2841 T10003 T9991 cc and,pH34U
R2842 T10004 T9991 conj pH34L,pH34U
R2843 T10005 T10004 punct -,pH34L
R2844 T10006 T10004 nummod 9000,pH34L
R2845 T10007 T10008 punct (,GATAAGCTGCTGCCAAAAGACAAG
R2846 T10008 T10004 parataxis GATAAGCTGCTGCCAAAAGACAAG,pH34L
R2847 T10009 T10008 nummod 5,GATAAGCTGCTGCCAAAAGACAAG
R2848 T10010 T10009 punct ',5
R2849 T10011 T10008 punct -,GATAAGCTGCTGCCAAAAGACAAG
R2850 T10012 T10008 punct -,GATAAGCTGCTGCCAAAAGACAAG
R2851 T10013 T10008 nummod 3,GATAAGCTGCTGCCAAAAGACAAG
R2852 T10014 T10008 punct ',GATAAGCTGCTGCCAAAAGACAAG
R2853 T10015 T10008 punct ),GATAAGCTGCTGCCAAAAGACAAG
R2854 T10016 T9986 punct .,amplified
R2855 T10018 T10019 det The,probe
R2856 T10019 T10020 nsubj probe,hybridized
R2857 T10021 T10020 prep to,hybridized
R2858 T10022 T10023 det an,band
R2859 T10023 T10021 pobj band,to
R2860 T10024 T10025 nummod 11.5,kbp
R2861 T10025 T10023 compound kbp,band
R2862 T10026 T10023 prep from,band
R2863 T10027 T10028 det the,locus
R2864 T10028 T10026 pobj locus,from
R2865 T10029 T10030 amod wild,type
R2866 T10030 T10028 compound type,locus
R2867 T10031 T10030 punct -,type
R2868 T10032 T10023 punct ", ",band
R2869 T10033 T10034 det a,band
R2870 T10034 T10023 conj band,band
R2871 T10035 T10036 nummod 12.5,kbp
R2872 T10036 T10034 compound kbp,band
R2873 T10037 T10034 prep from,band
R2874 T10038 T10039 det the,locus
R2875 T10039 T10037 pobj locus,from
R2876 T10040 T10041 compound β,geo
R2877 T10041 T10039 compound geo,locus
R2878 T10042 T10041 punct -,geo
R2879 T10043 T10034 punct ", ",band
R2880 T10044 T10034 cc and,band
R2881 T10045 T10046 det a,band
R2882 T10046 T10034 conj band,band
R2883 T10047 T10048 nummod 9.5,kbp
R2884 T10048 T10046 compound kbp,band
R2885 T10049 T10046 prep from,band
R2886 T10050 T10051 det the,locus
R2887 T10051 T10049 pobj locus,from
R2888 T10052 T10051 compound HygR,locus
R2889 T10053 T10020 punct .,hybridized
R2891 T10415 T10414 prep of,Generation
R2892 T10416 T10417 amod anti-ESG1,antibodies
R2893 T10417 T10415 pobj antibodies,of
R2894 T10418 T10417 amod polyclonal,antibodies
R2895 T10420 T10421 det The,sequence
R2896 T10421 T10423 nsubjpass sequence,amplified
R2897 T10422 T10421 amod coding,sequence
R2898 T10424 T10421 prep of,sequence
R2899 T10425 T10424 pobj Esg1,of
R2900 T10426 T10423 auxpass was,amplified
R2901 T10427 T10423 agent by,amplified
R2902 T10428 T10427 pobj PCR,by
R2903 T10429 T10423 prep with,amplified
R2904 T10430 T10431 det the,primers
R2905 T10431 T10429 pobj primers,with
R2906 T10432 T10433 nmod pH34,s
R2907 T10433 T10431 nmod s,primers
R2908 T10434 T10433 punct -,s
R2909 T10435 T10433 nmod gw,s
R2910 T10436 T10433 punct -,s
R2911 T10437 T10438 punct (,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2912 T10438 T10433 parataxis AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA,s
R2913 T10439 T10438 nummod 5,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2914 T10440 T10439 punct ',5
R2915 T10441 T10438 punct -,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2916 T10442 T10438 punct -,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2917 T10443 T10438 nummod 3,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2918 T10444 T10438 punct ',AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2919 T10445 T10438 punct ),AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2920 T10446 T10433 cc and,s
R2921 T10447 T10448 compound pH34,as
R2922 T10448 T10433 conj as,s
R2923 T10449 T10448 punct -,as
R2924 T10450 T10448 compound gw,as
R2925 T10451 T10448 punct -,as
R2926 T10452 T10453 punct (,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2927 T10453 T10448 parataxis AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA,as
R2928 T10454 T10453 nummod 5,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2929 T10455 T10454 punct ',5
R2930 T10456 T10453 punct -,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2931 T10457 T10453 punct -,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2932 T10458 T10453 nummod 3,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2933 T10459 T10453 punct ',AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2934 T10460 T10453 punct ),AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2935 T10461 T10423 punct .,amplified
R2936 T10463 T10464 aux To,construct
R2937 T10464 T10465 advcl construct,subcloned
R2938 T10466 T10467 compound pDONR,pH34
R2939 T10467 T10464 dobj pH34,construct
R2940 T10468 T10467 punct -,pH34
R2941 T10469 T10465 punct ", ",subcloned
R2942 T10470 T10471 det the,product
R2943 T10471 T10465 nsubjpass product,subcloned
R2944 T10472 T10471 amod resulting,product
R2945 T10473 T10471 compound PCR,product
R2946 T10474 T10465 auxpass was,subcloned
R2947 T10475 T10465 prep into,subcloned
R2948 T10476 T10475 pobj pDONR201,into
R2949 T10477 T10478 punct (,Invitrogen
R2950 T10478 T10476 parataxis Invitrogen,pDONR201
R2951 T10479 T10478 punct ),Invitrogen
R2952 T10480 T10465 punct .,subcloned
R2953 T10482 T10483 compound pDONR,pH34
R2954 T10483 T10485 nsubjpass pH34,interacted
R2955 T10484 T10483 punct -,pH34
R2956 T10486 T10485 auxpass was,interacted
R2957 T10487 T10485 prep with,interacted
R2958 T10488 T10487 pobj pDEST17,with
R2959 T10489 T10490 punct (,Invitrogen
R2960 T10490 T10488 parataxis Invitrogen,pDEST17
R2961 T10491 T10490 punct ),Invitrogen
R2962 T10492 T10485 prep by,interacted
R2963 T10493 T10494 compound LR,recombination
R2964 T10494 T10492 pobj recombination,by
R2965 T10495 T10485 punct .,interacted
R2966 T10497 T10498 prep After,induced
R2967 T10499 T10497 pobj introduction,After
R2968 T10500 T10499 prep of,introduction
R2969 T10501 T10502 det the,vector
R2970 T10502 T10500 pobj vector,of
R2971 T10503 T10502 amod resulting,vector
R2972 T10504 T10502 compound expression,vector
R2973 T10505 T10506 compound pDEST17,pH34
R2974 T10506 T10502 appos pH34,vector
R2975 T10507 T10506 punct -,pH34
R2976 T10508 T10499 prep into,introduction
R2977 T10509 T10510 compound BL21,AI
R2978 T10510 T10508 pobj AI,into
R2979 T10511 T10510 punct -,AI
R2980 T10512 T10510 nmod E.,AI
R2981 T10513 T10510 nmod coli,AI
R2982 T10514 T10515 punct (,Invitrogen
R2983 T10515 T10499 parataxis Invitrogen,introduction
R2984 T10516 T10515 punct ),Invitrogen
R2985 T10517 T10498 punct ", ",induced
R2986 T10518 T10519 amod recombinant,protein
R2987 T10519 T10520 compound protein,production
R2988 T10520 T10498 nsubjpass production,induced
R2989 T10521 T10498 auxpass was,induced
R2990 T10522 T10498 prep according,induced
R2991 T10523 T10522 prep to,according
R2992 T10524 T10525 det the,manufacture
R2993 T10525 T10526 poss manufacture,protocol
R2994 T10526 T10523 pobj protocol,to
R2995 T10527 T10525 case 's,manufacture
R2996 T10528 T10498 punct .,induced
R2997 T10530 T10531 npadvmod Histidine,tagged
R2998 T10531 T10533 amod tagged,ESG1
R2999 T10532 T10531 punct -,tagged
R3000 T10533 T10534 nsubjpass ESG1,purified
R3001 T10535 T10534 auxpass was,purified
R3002 T10536 T10534 advcl using,purified
R3003 T10537 T10538 nmod Ni,agarose
R3004 T10538 T10536 dobj agarose,using
R3005 T10539 T10537 punct -,Ni
R3006 T10540 T10537 amod nitrilotriacetic,Ni
R3007 T10541 T10538 compound acid,agarose
R3008 T10542 T10543 punct (,Qiagen
R3009 T10543 T10538 parataxis Qiagen,agarose
R3010 T10544 T10543 punct ),Qiagen
R3011 T10545 T10536 prep under,using
R3012 T10546 T10547 compound denaturing,conditions
R3013 T10547 T10545 pobj conditions,under
R3014 T10548 T10536 prep in,using
R3015 T10549 T10550 det the,presence
R3016 T10550 T10548 pobj presence,in
R3017 T10551 T10550 prep of,presence
R3018 T10552 T10553 nummod 8,M
R3019 T10553 T10554 compound M,urea
R3020 T10554 T10551 pobj urea,of
R3021 T10555 T10534 punct .,purified
R3022 T10557 T10558 prep After,injected
R3023 T10559 T10557 pobj dialysis,After
R3024 T10560 T10559 prep against,dialysis
R3025 T10561 T10562 nummod 6,M
R3026 T10562 T10563 compound M,urea
R3027 T10563 T10560 pobj urea,against
R3028 T10564 T10558 punct ", ",injected
R3029 T10565 T10566 det the,proteins
R3030 T10566 T10558 nsubjpass proteins,injected
R3031 T10567 T10566 amod recombinant,proteins
R3032 T10568 T10558 auxpass were,injected
R3033 T10569 T10558 prep into,injected
R3034 T10570 T10571 nmod New,Zealand
R3035 T10571 T10572 nmod Zealand,rabbits
R3036 T10572 T10569 pobj rabbits,into
R3037 T10573 T10572 amod White,rabbits
R3038 T10574 T10575 aux to,generate
R3039 T10575 T10558 advcl generate,injected
R3040 T10576 T10577 amod anti-ESG1,antibodies
R3041 T10577 T10575 dobj antibodies,generate
R3042 T10578 T10577 amod polyclonal,antibodies
R3043 T10579 T10558 punct .,injected
R3044 T10856 T10857 compound Western,blot
R3045 T10859 T10860 prep After,separated
R3046 T10861 T10859 pobj preparation,After
R3047 T10862 T10861 prep of,preparation
R3048 T10863 T10864 compound ES,extracts
R3049 T10864 T10862 pobj extracts,of
R3050 T10865 T10864 compound cell,extracts
R3051 T10866 T10861 prep with,preparation
R3052 T10867 T10868 compound M,Per
R3053 T10868 T10866 pobj Per,with
R3054 T10869 T10868 punct -,Per
R3055 T10870 T10871 punct (,Pierce
R3056 T10871 T10868 parataxis Pierce,Per
R3057 T10872 T10871 punct ),Pierce
R3058 T10873 T10860 punct ", ",separated
R3059 T10874 T10875 amod cellular,proteins
R3060 T10875 T10860 nsubjpass proteins,separated
R3061 T10876 T10860 auxpass were,separated
R3062 T10877 T10860 prep on,separated
R3063 T10878 T10879 nmod sodium,sulfate
R3064 T10879 T10881 nmod sulfate,gels
R3065 T10880 T10879 nmod dodecyl,sulfate
R3066 T10881 T10877 pobj gels,on
R3067 T10882 T10879 punct (,sulfate
R3068 T10883 T10879 appos SDS,sulfate
R3069 T10884 T10881 punct ),gels
R3070 T10885 T10881 punct -,gels
R3071 T10886 T10887 nummod 14,%
R3072 T10887 T10881 compound %,gels
R3073 T10888 T10881 compound polyacrylamide,gels
R3074 T10889 T10860 cc and,separated
R3075 T10890 T10860 conj transferred,separated
R3076 T10891 T10890 prep to,transferred
R3077 T10892 T10893 compound nitrocellulose,membranes
R3078 T10893 T10891 pobj membranes,to
R3079 T10894 T10895 punct (,Millipore
R3080 T10895 T10890 parataxis Millipore,transferred
R3081 T10896 T10895 punct ),Millipore
R3082 T10897 T10860 punct .,separated
R3083 T10899 T10900 nsubjpass Membranes,incubated
R3084 T10901 T10900 auxpass were,incubated
R3085 T10902 T10900 prep with,incubated
R3086 T10903 T10904 nmod anti-ESG1,antibodies
R3087 T10904 T10902 pobj antibodies,with
R3088 T10905 T10906 punct (,dilution
R3089 T10906 T10903 parataxis dilution,anti-ESG1
R3090 T10907 T10908 quantmod 1,500
R3091 T10908 T10906 nummod 500,dilution
R3092 T10909 T10908 punct /,500
R3093 T10910 T10906 punct ),dilution
R3094 T10911 T10903 punct ", ",anti-ESG1
R3095 T10912 T10903 conj anti-Oct3,anti-ESG1
R3096 T10913 T10912 punct /,anti-Oct3
R3097 T10914 T10912 nummod 4,anti-Oct3
R3098 T10915 T10916 punct (,Biotechnology
R3099 T10916 T10912 parataxis Biotechnology,anti-Oct3
R3100 T10917 T10918 quantmod 1,500
R3101 T10918 T10916 dep 500,Biotechnology
R3102 T10919 T10918 punct /,500
R3103 T10920 T10916 punct ;,Biotechnology
R3104 T10921 T10916 compound Santa,Biotechnology
R3105 T10922 T10916 compound Cruz,Biotechnology
R3106 T10923 T10916 punct ),Biotechnology
R3107 T10924 T10912 punct ", ",anti-Oct3
R3108 T10925 T10912 conj anti-CDK4,anti-Oct3
R3109 T10926 T10927 punct (,Biotechnology
R3110 T10927 T10925 parataxis Biotechnology,anti-CDK4
R3111 T10928 T10929 quantmod 1,200
R3112 T10929 T10927 dep 200,Biotechnology
R3113 T10930 T10929 punct /,200
R3114 T10931 T10927 punct ;,Biotechnology
R3115 T10932 T10927 compound Santa,Biotechnology
R3116 T10933 T10927 compound Cruz,Biotechnology
R3117 T10934 T10927 punct ),Biotechnology
R3118 T10935 T10925 punct ", ",anti-CDK4
R3119 T10936 T10925 cc and,anti-CDK4
R3120 T10937 T10925 conj anti-GFP,anti-CDK4
R3121 T10938 T10939 punct (,MBL
R3122 T10939 T10937 parataxis MBL,anti-GFP
R3123 T10940 T10941 quantmod 1,1000
R3124 T10941 T10939 dep 1000,MBL
R3125 T10942 T10941 punct /,1000
R3126 T10943 T10939 punct ;,MBL
R3127 T10944 T10939 punct ),MBL
R3128 T10945 T10904 amod primary,antibodies
R3129 T10946 T10900 punct .,incubated
R3130 T10948 T10949 compound Horseradish,peroxidase
R3131 T10949 T10950 npadvmod peroxidase,conjugated
R3132 T10950 T10952 amod conjugated,immunoglobulins
R3133 T10951 T10950 punct -,conjugated
R3134 T10952 T10956 nsubjpass immunoglobulins,used
R3135 T10953 T10952 amod anti-rabbit,immunoglobulins
R3136 T10954 T10953 cc and,anti-rabbit
R3137 T10955 T10953 conj anti-mouse,anti-rabbit
R3138 T10957 T10958 punct (,Signaling
R3139 T10958 T10952 parataxis Signaling,immunoglobulins
R3140 T10959 T10960 quantmod 1,5000
R3141 T10960 T10958 dep 5000,Signaling
R3142 T10961 T10960 punct /,5000
R3143 T10962 T10958 punct ;,Signaling
R3144 T10963 T10958 compound Cell,Signaling
R3145 T10964 T10958 punct ),Signaling
R3146 T10965 T10956 auxpass were,used
R3147 T10966 T10967 aux to,detect
R3148 T10967 T10956 advcl detect,used
R3149 T10968 T10969 compound antibody,binding
R3150 T10969 T10967 dobj binding,detect
R3151 T10970 T10956 punct .,used
R3152 T10972 T10973 nsubj We,visualized
R3153 T10974 T10975 amod bound,antibody
R3154 T10975 T10973 dobj antibody,visualized
R3155 T10976 T10973 prep with,visualized
R3156 T10977 T10978 det an,System
R3157 T10978 T10976 pobj System,with
R3158 T10979 T10978 compound ECL,System
R3159 T10980 T10981 compound Western,Blotting
R3160 T10981 T10978 compound Blotting,System
R3161 T10982 T10978 compound Detection,System
R3162 T10983 T10984 punct (,Amersham
R3163 T10984 T10978 parataxis Amersham,System
R3164 T10985 T10984 punct ),Amersham
R3165 T10986 T10973 punct .,visualized
R3168 T11477 T11476 prep of,Derivation
R3169 T11478 T11479 npadvmod ESG1,deficient
R3170 T11479 T11481 amod deficient,cells
R3171 T11480 T11479 punct -,deficient
R3172 T11481 T11477 pobj cells,of
R3173 T11482 T11481 compound ES,cells
R3174 T11483 T11476 prep from,Derivation
R3175 T11484 T11483 pobj blastocysts,from
R3176 T11486 T11487 nmod Esg1,mice
R3177 T11487 T11498 nsubjpass mice,injected
R3178 T11488 T11486 punct +,Esg1
R3179 T11489 T11486 punct /,Esg1
R3180 T11490 T11486 punct -,Esg1
R3181 T11491 T11486 cc or,Esg1
R3182 T11492 T11486 conj ESG1,Esg1
R3183 T11493 T11492 punct -,ESG1
R3184 T11494 T11492 punct /,ESG1
R3185 T11495 T11492 punct -,ESG1
R3186 T11496 T11487 nmod mutant,mice
R3187 T11497 T11487 amod female,mice
R3188 T11499 T11498 auxpass were,injected
R3189 T11500 T11498 prep with,injected
R3190 T11501 T11500 pobj Tamoxifen,with
R3191 T11502 T11503 punct (,μg
R3192 T11503 T11501 parataxis μg,Tamoxifen
R3193 T11504 T11503 nummod 10,μg
R3194 T11505 T11503 punct ),μg
R3195 T11506 T11501 cc and,Tamoxifen
R3196 T11507 T11508 compound Depo,provera
R3197 T11508 T11501 conj provera,Tamoxifen
R3198 T11509 T11508 punct -,provera
R3199 T11510 T11511 punct (,mg
R3200 T11511 T11508 parataxis mg,provera
R3201 T11512 T11511 nummod 1,mg
R3202 T11513 T11511 punct ),mg
R3203 T11514 T11498 advmod subcutaneously,injected
R3204 T11515 T11498 prep on,injected
R3205 T11516 T11517 det the,day
R3206 T11517 T11515 pobj day,on
R3207 T11518 T11517 amod third,day
R3208 T11519 T11517 prep of,day
R3209 T11520 T11519 pobj pregnancy,of
R3210 T11521 T11498 punct .,injected
R3211 T11523 T11524 nummod Four,days
R3212 T11524 T11525 npadvmod days,later
R3213 T11525 T11526 advmod later,flushed
R3214 T11527 T11526 punct ", ",flushed
R3215 T11528 T11526 nsubjpass embryos,flushed
R3216 T11529 T11528 prep in,embryos
R3217 T11530 T11529 pobj diapause,in
R3218 T11531 T11526 auxpass were,flushed
R3219 T11532 T11526 prep out,flushed
R3220 T11533 T11532 prep of,out
R3221 T11534 T11535 det the,uterus
R3222 T11535 T11533 pobj uterus,of
R3223 T11536 T11526 cc and,flushed
R3224 T11537 T11526 conj cultured,flushed
R3225 T11538 T11537 prep on,cultured
R3226 T11539 T11540 compound STO,cells
R3227 T11540 T11538 pobj cells,on
R3228 T11541 T11540 compound feeder,cells
R3229 T11542 T11537 prep in,cultured
R3230 T11543 T11544 nummod four,well
R3231 T11544 T11546 compound well,plates
R3232 T11545 T11544 punct -,well
R3233 T11546 T11542 pobj plates,in
R3234 T11547 T11537 prep in,cultured
R3235 T11548 T11549 poss Dulbecco,Medium
R3236 T11549 T11547 pobj Medium,in
R3237 T11550 T11548 case 's,Dulbecco
R3238 T11551 T11549 amod Modified,Medium
R3239 T11552 T11549 compound Eagle,Medium
R3240 T11553 T11549 punct (,Medium
R3241 T11554 T11549 appos DMEM,Medium
R3242 T11555 T11549 punct ),Medium
R3243 T11556 T11549 acl supplemented,Medium
R3244 T11557 T11556 prep with,supplemented
R3245 T11558 T11559 nummod 20,%
R3246 T11559 T11560 nmod %,Serum
R3247 T11560 T11557 pobj Serum,with
R3248 T11561 T11560 amod Fetal,Serum
R3249 T11562 T11560 amod Bovine,Serum
R3250 T11563 T11564 punct (,Hyclone
R3251 T11564 T11560 parataxis Hyclone,Serum
R3252 T11565 T11564 punct ),Hyclone
R3253 T11566 T11560 punct ", ",Serum
R3254 T11567 T11568 nummod 0.1,mM
R3255 T11568 T11569 nmod mM,Acids
R3256 T11569 T11560 conj Acids,Serum
R3257 T11570 T11569 amod Non-Essential,Acids
R3258 T11571 T11569 compound Amino,Acids
R3259 T11572 T11573 punct (,Invitrogen
R3260 T11573 T11569 parataxis Invitrogen,Acids
R3261 T11574 T11573 punct ),Invitrogen
R3262 T11575 T11569 punct ", ",Acids
R3263 T11576 T11577 nummod 2,mM
R3264 T11577 T11578 compound mM,glutamine
R3265 T11578 T11569 conj glutamine,Acids
R3266 T11579 T11578 compound L,glutamine
R3267 T11580 T11578 punct -,glutamine
R3268 T11581 T11582 punct (,Invitrogen
R3269 T11582 T11578 parataxis Invitrogen,glutamine
R3270 T11583 T11582 punct ),Invitrogen
R3271 T11584 T11578 punct ", ",glutamine
R3272 T11585 T11586 nummod 50,U
R3273 T11586 T11587 nmod U,Streptomysin
R3274 T11587 T11578 conj Streptomysin,glutamine
R3275 T11588 T11589 punct /,ml
R3276 T11589 T11586 prep ml,U
R3277 T11590 T11587 compound Penicillin,Streptomysin
R3278 T11591 T11587 punct -,Streptomysin
R3279 T11592 T11593 punct (,Invitrogen
R3280 T11593 T11587 parataxis Invitrogen,Streptomysin
R3281 T11594 T11593 punct ),Invitrogen
R3282 T11595 T11587 punct ", ",Streptomysin
R3283 T11596 T11587 cc and,Streptomysin
R3284 T11597 T11598 nummod 0.11,mM
R3285 T11598 T11599 nmod mM,mercaptoethanol
R3286 T11599 T11587 conj mercaptoethanol,Streptomysin
R3287 T11600 T11599 nummod 2,mercaptoethanol
R3288 T11601 T11599 punct -,mercaptoethanol
R3289 T11602 T11603 punct (,Invitrogen
R3290 T11603 T11599 parataxis Invitrogen,mercaptoethanol
R3291 T11604 T11603 punct ),Invitrogen
R3292 T11605 T11526 punct .,flushed
R3293 T11607 T11608 prep After,harvested
R3294 T11609 T11610 nummod six,days
R3295 T11610 T11607 pobj days,After
R3296 T11611 T11608 punct ", ",harvested
R3297 T11612 T11613 det the,mass
R3298 T11613 T11608 nsubjpass mass,harvested
R3299 T11614 T11613 amod central,mass
R3300 T11615 T11613 prep of,mass
R3301 T11616 T11617 det each,explant
R3302 T11617 T11615 pobj explant,of
R3303 T11618 T11608 auxpass was,harvested
R3304 T11619 T11608 punct ", ",harvested
R3305 T11620 T11608 conj rinsed,harvested
R3306 T11621 T11620 prep in,rinsed
R3307 T11622 T11621 pobj PBS,in
R3308 T11623 T11620 punct ", ",rinsed
R3309 T11624 T11620 cc and,rinsed
R3310 T11625 T11620 conj placed,rinsed
R3311 T11626 T11625 prep in,placed
R3312 T11627 T11628 det a,drop
R3313 T11628 T11626 pobj drop,in
R3314 T11629 T11628 prep of,drop
R3315 T11630 T11629 pobj trypsin,of
R3316 T11631 T11625 prep for,placed
R3317 T11632 T11633 det a,minutes
R3318 T11633 T11631 pobj minutes,for
R3319 T11634 T11633 amod few,minutes
R3320 T11635 T11608 punct .,harvested
R3321 T11637 T11638 det The,mass
R3322 T11638 T11640 nsubjpass mass,collected
R3323 T11639 T11638 compound cell,mass
R3324 T11641 T11640 auxpass was,collected
R3325 T11642 T11640 prep with,collected
R3326 T11643 T11644 det a,pipette
R3327 T11644 T11642 pobj pipette,with
R3328 T11645 T11646 advmod finely,drawn
R3329 T11646 T11644 amod drawn,pipette
R3330 T11647 T11646 punct -,drawn
R3331 T11648 T11646 prt out,drawn
R3332 T11649 T11644 compound Pasteur,pipette
R3333 T11650 T11644 acl preloaded,pipette
R3334 T11651 T11650 prep with,preloaded
R3335 T11652 T11651 pobj medium,with
R3336 T11653 T11640 punct ", ",collected
R3337 T11654 T11640 advcl ensuring,collected
R3338 T11655 T11656 amod minimal,carryover
R3339 T11656 T11654 dobj carryover,ensuring
R3340 T11657 T11656 prep of,carryover
R3341 T11658 T11659 det the,trypsin
R3342 T11659 T11657 pobj trypsin,of
R3343 T11660 T11640 punct .,collected
R3344 T11662 T11663 det The,cells
R3345 T11663 T11664 nsubjpass cells,transfered
R3346 T11665 T11664 auxpass were,transfered
R3347 T11666 T11664 advmod gently,transfered
R3348 T11667 T11664 prep into,transfered
R3349 T11668 T11669 det a,well
R3350 T11669 T11667 pobj well,into
R3351 T11670 T11669 amod fresh,well
R3352 T11671 T11664 prep with,transfered
R3353 T11672 T11673 nummod 20,%
R3354 T11673 T11674 nmod %,medium
R3355 T11674 T11671 pobj medium,with
R3356 T11675 T11676 npadvmod FBS,containing
R3357 T11676 T11674 amod containing,medium
R3358 T11677 T11676 punct -,containing
R3359 T11678 T11664 punct .,transfered
R3360 T11680 T11681 det The,colonies
R3361 T11681 T11686 nsubjpass colonies,passaged
R3362 T11682 T11681 amod resulting,colonies
R3363 T11683 T11681 amod primary,colonies
R3364 T11684 T11681 compound ES,colonies
R3365 T11685 T11681 compound cell,colonies
R3366 T11687 T11686 auxpass were,passaged
R3367 T11688 T11686 advmod individually,passaged
R3368 T11689 T11686 prep into,passaged
R3369 T11690 T11689 pobj wells,into
R3370 T11691 T11690 prep of,wells
R3371 T11692 T11693 nummod four,well
R3372 T11693 T11695 compound well,plates
R3373 T11694 T11693 punct -,well
R3374 T11695 T11691 pobj plates,of
R3375 T11696 T11690 acl containing,wells
R3376 T11697 T11698 compound STO,layers
R3377 T11698 T11696 dobj layers,containing
R3378 T11699 T11700 compound feeder,cell
R3379 T11700 T11698 compound cell,layers
R3380 T11701 T11686 punct .,passaged
R3381 T11703 T11704 advmod Thereafter,expanded
R3382 T11705 T11704 punct ", ",expanded
R3383 T11706 T11704 nsubjpass cells,expanded
R3384 T11707 T11704 auxpass were,expanded
R3385 T11708 T11704 prep by,expanded
R3386 T11709 T11708 pobj trypsinization,by
R3387 T11710 T11709 prep of,trypsinization
R3388 T11711 T11712 det the,culture
R3389 T11712 T11710 pobj culture,of
R3390 T11713 T11712 amod entire,culture
R3391 T11714 T11704 punct .,expanded
R3392 T11885 T11886 amod Total,RNA
R3393 T11886 T11887 nsubjpass RNA,labeled
R3394 T11888 T11886 prep from,RNA
R3395 T11889 T11890 amod wild,type
R3396 T11890 T11892 compound type,cells
R3397 T11891 T11890 punct -,type
R3398 T11892 T11888 pobj cells,from
R3399 T11893 T11892 compound ES,cells
R3400 T11894 T11892 cc and,cells
R3401 T11895 T11896 nmod ESG1,cells
R3402 T11896 T11892 conj cells,cells
R3403 T11897 T11895 punct -,ESG1
R3404 T11898 T11895 punct /,ESG1
R3405 T11899 T11895 punct -,ESG1
R3406 T11900 T11896 compound ES,cells
R3407 T11901 T11887 auxpass was,labeled
R3408 T11902 T11887 prep with,labeled
R3409 T11903 T11902 pobj Cy3,with
R3410 T11904 T11903 cc and,Cy3
R3411 T11905 T11903 conj Cy5,Cy3
R3412 T11906 T11887 punct ", ",labeled
R3413 T11907 T11887 advmod respectively,labeled
R3414 T11908 T11887 punct .,labeled
R3415 T11910 T11911 det The,samples
R3416 T11911 T11912 nsubjpass samples,hybridized
R3417 T11913 T11912 auxpass were,hybridized
R3418 T11914 T11912 prep to,hybridized
R3419 T11915 T11916 det a,Microarray
R3420 T11916 T11914 pobj Microarray,to
R3421 T11917 T11916 compound Mouse,Microarray
R3422 T11918 T11916 compound Development,Microarray
R3423 T11919 T11920 punct (,Algilent
R3424 T11920 T11916 parataxis Algilent,Microarray
R3425 T11921 T11920 punct ),Algilent
R3426 T11922 T11912 prep according,hybridized
R3427 T11923 T11922 prep to,according
R3428 T11924 T11925 det the,manufacturer
R3429 T11925 T11926 poss manufacturer,protocol
R3430 T11926 T11923 pobj protocol,to
R3431 T11927 T11925 case 's,manufacturer
R3432 T11928 T11912 punct .,hybridized
R3433 T11930 T11931 nsubjpass Arrays,scanned
R3434 T11932 T11931 auxpass were,scanned
R3435 T11933 T11931 prep with,scanned
R3436 T11934 T11935 det a,System
R3437 T11935 T11933 pobj System,with
R3438 T11936 T11935 compound G2565BA,System
R3439 T11937 T11935 compound Microarray,System
R3440 T11938 T11935 compound Scanner,System
R3441 T11939 T11940 punct (,Agilent
R3442 T11940 T11935 parataxis Agilent,System
R3443 T11941 T11940 punct ),Agilent
R3444 T11942 T11931 punct .,scanned
R3445 T11944 T11945 nsubjpass Hybridization,repeated
R3446 T11946 T11945 auxpass was,repeated
R3447 T11947 T11945 prep with,repeated
R3448 T11948 T11949 nummod two,clones
R3449 T11949 T11947 pobj clones,with
R3450 T11950 T11949 amod independent,clones
R3451 T11951 T11945 punct .,repeated
R3452 T11953 T11954 nsubjpass Data,analyzed
R3453 T11955 T11954 auxpass were,analyzed
R3454 T11956 T11954 prep with,analyzed
R3455 T11957 T11958 compound GeneSprings,software
R3456 T11958 T11956 pobj software,with
R3457 T11959 T11960 punct (,Genetics
R3458 T11960 T11958 parataxis Genetics,software
R3459 T11961 T11960 compound Silico,Genetics
R3460 T11962 T11960 punct ),Genetics
R3461 T11963 T11954 punct .,analyzed
R3462 T12056 T12055 acl identified,ESG1pseudogenes
R3463 T12057 T12056 agent by,identified
R3464 T12058 T12059 det a,search
R3465 T12059 T12057 pobj search,by
R3466 T12060 T12059 compound Blast,search
R3467 T12061 T12059 prep of,search
R3468 T12062 T12063 nmod mouse,databases
R3469 T12063 T12061 pobj databases,of
R3470 T12064 T12063 amod genomic,databases
R3471 T12065 T12055 punct .,ESG1pseudogenes
R3472 T12067 T12068 compound Substitution,mutations
R3473 T12068 T12069 nsubjpass mutations,indicated
R3474 T12070 T12069 auxpass are,indicated
R3475 T12071 T12069 agent by,indicated
R3476 T12072 T12073 amod black,lines
R3477 T12073 T12071 pobj lines,by
R3478 T12074 T12069 punct .,indicated
R3479 T12076 T12077 npadvmod Intron,like
R3480 T12077 T12079 amod like,sequences
R3481 T12078 T12077 punct -,like
R3482 T12079 T12081 nsubjpass sequences,indicated
R3483 T12080 T12079 compound gap,sequences
R3484 T12082 T12081 auxpass are,indicated
R3485 T12083 T12081 prep with,indicated
R3486 T12084 T12083 pobj triangles,with
R3487 T12085 T12081 punct .,indicated
R3488 T12087 T12088 amod Chromosomal,localizations
R3489 T12088 T12089 nsubjpass localizations,shown
R3490 T12090 T12089 auxpass are,shown
R3491 T12091 T12089 prep on,shown
R3492 T12092 T12093 det the,right
R3493 T12093 T12091 pobj right,on
R3494 T12094 T12089 punct .,shown
R3495 T12275 T12276 compound BAC,clones
R3496 T12276 T12277 nsubj clones,containing
R3497 T12278 T12279 det the,gene
R3498 T12279 T12277 dobj gene,containing
R3499 T12280 T12279 compound ESG1,gene
R3500 T12281 T12279 cc and,gene
R3501 T12282 T12283 det a,pseudogene
R3502 T12283 T12279 conj pseudogene,gene
R3503 T12284 T12283 compound duplication,pseudogene
R3504 T12285 T12286 punct (,PS
R3505 T12286 T12283 parataxis PS,pseudogene
R3506 T12287 T12286 punct ),PS
R3507 T12288 T12277 punct .,containing
R3508 T12290 T12291 meta A,Localization
R3509 T12292 T12290 punct ),A
R3510 T12293 T12291 prep of,Localization
R3511 T12294 T12295 det the,gene
R3512 T12295 T12293 pobj gene,of
R3513 T12296 T12295 cc and,gene
R3514 T12297 T12295 conj PS,gene
R3515 T12298 T12291 prep on,Localization
R3516 T12299 T12298 pobj chromosome,on
R3517 T12300 T12299 nummod 9,chromosome
R3518 T12301 T12291 punct .,Localization
R3519 T12303 T12304 meta B,revealed
R3520 T12305 T12303 punct ),B
R3521 T12306 T12307 compound Sequence,comparison
R3522 T12307 T12304 nsubj comparison,revealed
R3523 T12308 T12307 prep of,comparison
R3524 T12309 T12310 det the,gene
R3525 T12310 T12308 pobj gene,of
R3526 T12311 T12310 cc and,gene
R3527 T12312 T12310 conj PS,gene
R3528 T12313 T12314 mark that,are
R3529 T12314 T12304 ccomp are,revealed
R3530 T12315 T12314 nsubj these,are
R3531 T12316 T12314 acomp homologous,are
R3532 T12317 T12316 prep from,homologous
R3533 T12318 T12319 nummod eight,pairs
R3534 T12319 T12317 pobj pairs,from
R3535 T12320 T12319 compound base,pairs
R3536 T12321 T12319 advmod upstream,pairs
R3537 T12322 T12321 prep of,upstream
R3538 T12323 T12324 det the,exon
R3539 T12324 T12322 pobj exon,of
R3540 T12325 T12324 amod first,exon
R3541 T12326 T12317 prep to,from
R3542 T12327 T12328 nummod 675,bp
R3543 T12328 T12326 pobj bp,to
R3544 T12329 T12328 advmod downstream,bp
R3545 T12330 T12329 prep of,downstream
R3546 T12331 T12332 det the,exon
R3547 T12332 T12330 pobj exon,of
R3548 T12333 T12332 amod third,exon
R3549 T12334 T12304 punct .,revealed
R3550 T12336 T12337 compound Substitution,mutations
R3551 T12337 T12338 nsubjpass mutations,indicated
R3552 T12339 T12338 auxpass are,indicated
R3553 T12340 T12338 prep by,indicated
R3554 T12341 T12342 amod black,lines
R3555 T12342 T12340 pobj lines,by
R3556 T12343 T12338 punct .,indicated
R3557 T12345 T12346 det The,insertion
R3558 T12346 T12347 nsubjpass insertion,indicated
R3559 T12348 T12347 auxpass is,indicated
R3560 T12349 T12347 prep by,indicated
R3561 T12350 T12351 det an,triangle
R3562 T12351 T12349 pobj triangle,by
R3563 T12352 T12351 amod open,triangle
R3564 T12353 T12347 punct .,indicated
R3565 T12543 T12544 compound Promoter,enhancer
R3566 T12544 T12546 compound enhancer,activity
R3567 T12545 T12544 punct /,enhancer
R3568 T12547 T12546 prep of,activity
R3569 T12548 T12549 det the,gene
R3570 T12549 T12547 pobj gene,of
R3571 T12550 T12549 compound ESG1,gene
R3572 T12551 T12549 cc and,gene
R3573 T12552 T12549 conj pseudogene,gene
R3574 T12553 T12546 punct .,activity
R3575 T12555 T12556 compound DNA,fragments
R3576 T12556 T12557 nsubjpass fragments,transferred
R3577 T12558 T12556 prep of,fragments
R3578 T12559 T12560 punct ~,6
R3579 T12560 T12561 nummod 6,kbp
R3580 T12561 T12558 pobj kbp,of
R3581 T12562 T12561 acl isolated,kbp
R3582 T12563 T12562 prep from,isolated
R3583 T12564 T12565 det the,regions
R3584 T12565 T12563 pobj regions,from
R3585 T12566 T12565 nummod 5,regions
R3586 T12567 T12566 punct ',5
R3587 T12568 T12565 compound flanking,regions
R3588 T12569 T12565 prep of,regions
R3589 T12570 T12571 det the,gene
R3590 T12571 T12569 pobj gene,of
R3591 T12572 T12571 cc and,gene
R3592 T12573 T12571 conj PS,gene
R3593 T12574 T12557 auxpass were,transferred
R3594 T12575 T12557 prep into,transferred
R3595 T12576 T12577 compound luciferase,reporter
R3596 T12577 T12578 compound reporter,plasmids
R3597 T12578 T12575 pobj plasmids,into
R3598 T12579 T12557 punct .,transferred
R3599 T12581 T12582 nsubj We,introduced
R3600 T12583 T12584 det the,genes
R3601 T12584 T12582 dobj genes,introduced
R3602 T12585 T12584 compound reporter,genes
R3603 T12586 T12582 prep into,introduced
R3604 T12587 T12588 amod undifferentiated,cells
R3605 T12588 T12586 pobj cells,into
R3606 T12589 T12588 compound ES,cells
R3607 T12590 T12591 punct (,columns
R3608 T12591 T12588 parataxis columns,cells
R3609 T12592 T12591 amod open,columns
R3610 T12593 T12591 punct ),columns
R3611 T12594 T12588 punct ", ",cells
R3612 T12595 T12596 amod retinoic,acid
R3613 T12596 T12597 npadvmod acid,treated
R3614 T12597 T12599 amod treated,cells
R3615 T12598 T12597 punct -,treated
R3616 T12599 T12588 conj cells,cells
R3617 T12600 T12599 compound ES,cells
R3618 T12601 T12602 punct (,columns
R3619 T12602 T12599 parataxis columns,cells
R3620 T12603 T12602 amod grey,columns
R3621 T12604 T12602 punct ),columns
R3622 T12605 T12599 punct ", ",cells
R3623 T12606 T12599 cc and,cells
R3624 T12607 T12608 compound NIH3T3,cells
R3625 T12608 T12599 conj cells,cells
R3626 T12609 T12610 punct (,columns
R3627 T12610 T12608 parataxis columns,cells
R3628 T12611 T12610 amod closed,columns
R3629 T12612 T12610 punct ),columns
R3630 T12613 T12582 punct .,introduced
R3631 T12615 T12616 nsubj Data,represent
R3632 T12617 T12618 det the,averages
R3633 T12618 T12616 dobj averages,represent
R3634 T12619 T12618 cc and,averages
R3635 T12620 T12621 amod standard,deviations
R3636 T12621 T12618 conj deviations,averages
R3637 T12622 T12618 prep of,averages
R3638 T12623 T12624 nummod three,experiments
R3639 T12624 T12622 pobj experiments,of
R3640 T12625 T12624 amod independent,experiments
R3641 T12626 T12616 punct .,represent
R3642 T13078 T13079 amod Targeted,disruption
R3643 T13080 T13079 prep of,disruption
R3644 T13081 T13082 det the,gene
R3645 T13082 T13080 pobj gene,of
R3646 T13083 T13082 compound mouse,gene
R3647 T13084 T13082 compound ESG1,gene
R3648 T13085 T13079 punct .,disruption
R3649 T13087 T13088 meta A,strategy
R3650 T13089 T13087 punct ),A
R3651 T13090 T13088 compound Targeting,strategy
R3652 T13091 T13088 punct .,strategy
R3653 T13093 T13094 amod Homologous,regions
R3654 T13094 T13095 nsubjpass regions,indicated
R3655 T13096 T13095 auxpass are,indicated
R3656 T13097 T13095 agent by,indicated
R3657 T13098 T13099 amod thick,lines
R3658 T13099 T13097 pobj lines,by
R3659 T13100 T13095 punct .,indicated
R3660 T13102 T13103 compound Recognition,sites
R3661 T13103 T13104 nsubjpass sites,shown
R3662 T13105 T13103 prep of,sites
R3663 T13106 T13105 pobj PstI,of
R3664 T13107 T13108 punct (,P
R3665 T13108 T13106 parataxis P,PstI
R3666 T13109 T13108 punct ),P
R3667 T13110 T13106 cc and,PstI
R3668 T13111 T13106 conj SpeI,PstI
R3669 T13112 T13113 punct (,S
R3670 T13113 T13111 parataxis S,SpeI
R3671 T13114 T13113 punct ),S
R3672 T13115 T13103 punct ", ",sites
R3673 T13116 T13117 dep which,used
R3674 T13117 T13103 relcl used,sites
R3675 T13118 T13117 auxpass were,used
R3676 T13119 T13117 prep for,used
R3677 T13120 T13121 compound Southern,blot
R3678 T13121 T13122 compound blot,analyses
R3679 T13122 T13119 pobj analyses,for
R3680 T13123 T13104 punct ", ",shown
R3681 T13124 T13104 auxpass are,shown
R3682 T13125 T13104 punct .,shown
R3683 T13127 T13128 det The,A
R3684 T13128 T13133 nsubjpass A,inserted
R3685 T13129 T13128 nmod gene,A
R3686 T13130 T13128 amod encoding,A
R3687 T13131 T13128 compound diphtheria,A
R3688 T13132 T13128 compound toxin,A
R3689 T13134 T13128 punct (,A
R3690 T13135 T13128 appos DTA,A
R3691 T13136 T13133 punct ),inserted
R3692 T13137 T13133 auxpass was,inserted
R3693 T13138 T13133 prep at,inserted
R3694 T13139 T13140 det the,end
R3695 T13140 T13138 pobj end,at
R3696 T13141 T13140 nummod 3,end
R3697 T13142 T13141 punct ',3
R3698 T13143 T13140 prep of,end
R3699 T13144 T13145 det the,vectors
R3700 T13145 T13143 pobj vectors,of
R3701 T13146 T13145 compound targeting,vectors
R3702 T13147 T13148 aux to,facilitate
R3703 T13148 T13133 advcl facilitate,inserted
R3704 T13149 T13150 amod negative,selection
R3705 T13150 T13148 dobj selection,facilitate
R3706 T13151 T13133 punct .,inserted
R3707 T13153 T13154 meta B,analyses
R3708 T13155 T13153 punct ),B
R3709 T13156 T13157 compound Southern,blot
R3710 T13157 T13154 compound blot,analyses
R3711 T13158 T13154 acl confirming,analyses
R3712 T13159 T13160 amod homologous,recombination
R3713 T13160 T13158 dobj recombination,confirming
R3714 T13161 T13154 punct .,analyses
R3715 T13164 T13163 punct ", ",WT
R3716 T13165 T13166 amod wild,type
R3717 T13166 T13168 compound type,cells
R3718 T13167 T13166 punct -,type
R3719 T13168 T13163 appos cells,WT
R3720 T13169 T13168 compound ES,cells
R3721 T13170 T13163 punct ;,WT
R3722 T13171 T13163 appos β,WT
R3723 T13172 T13171 punct ", ",β
R3724 T13173 T13174 nmod β,geo
R3725 T13174 T13176 nmod geo,cells
R3726 T13175 T13174 punct -,geo
R3727 T13176 T13171 appos cells,β
R3728 T13177 T13174 punct +,geo
R3729 T13178 T13174 punct /,geo
R3730 T13179 T13174 punct -,geo
R3731 T13180 T13176 compound ES,cells
R3732 T13181 T13163 punct ;,WT
R3733 T13182 T13163 appos H,WT
R3734 T13183 T13182 punct ", ",H
R3735 T13184 T13185 nmod HygR,cells
R3736 T13185 T13182 appos cells,H
R3737 T13186 T13184 punct +,HygR
R3738 T13187 T13184 punct /,HygR
R3739 T13188 T13184 punct -,HygR
R3740 T13189 T13185 compound ES,cells
R3741 T13190 T13163 punct ;,WT
R3742 T13191 T13192 punct -,-
R3743 T13192 T13163 appos -,WT
R3744 T13193 T13192 punct /,-
R3745 T13194 T13192 punct ", ",-
R3746 T13195 T13196 npadvmod ESG1,null
R3747 T13196 T13198 amod null,cells
R3748 T13197 T13196 punct -,null
R3749 T13198 T13192 appos cells,-
R3750 T13199 T13198 compound ES,cells
R3751 T13200 T13163 punct .,WT
R3752 T13202 T13203 nsubj Numbers,indicate
R3753 T13204 T13205 compound clone,numbers
R3754 T13205 T13203 dobj numbers,indicate
R3755 T13206 T13203 punct .,indicate
R3756 T13208 T13209 meta C,analyses
R3757 T13210 T13208 punct ),C
R3758 T13211 T13212 nmod Northern,blot
R3759 T13212 T13209 nmod blot,analyses
R3760 T13213 T13214 punct (,upper
R3761 T13214 T13212 parataxis upper,blot
R3762 T13215 T13214 punct ),upper
R3763 T13216 T13212 cc and,blot
R3764 T13217 T13218 compound western,blot
R3765 T13218 T13212 conj blot,blot
R3766 T13219 T13220 punct (,lower
R3767 T13220 T13218 parataxis lower,blot
R3768 T13221 T13220 punct ),lower
R3769 T13222 T13209 prep of,analyses
R3770 T13223 T13224 amod wild,type
R3771 T13224 T13226 compound type,cells
R3772 T13225 T13224 punct -,type
R3773 T13226 T13222 pobj cells,of
R3774 T13227 T13226 compound ES,cells
R3775 T13228 T13229 punct (,WT
R3776 T13229 T13226 parataxis WT,cells
R3777 T13230 T13229 punct ),WT
R3778 T13231 T13226 punct ", ",cells
R3779 T13232 T13233 npadvmod ESG1,null
R3780 T13233 T13235 amod null,cells
R3781 T13234 T13233 punct -,null
R3782 T13235 T13226 conj cells,cells
R3783 T13236 T13235 compound ES,cells
R3784 T13237 T13238 punct (,clones
R3785 T13238 T13235 parataxis clones,cells
R3786 T13239 T13240 punct -,-
R3787 T13240 T13238 punct -,clones
R3788 T13241 T13240 punct /,-
R3789 T13242 T13238 punct ", ",clones
R3790 T13243 T13238 nummod three,clones
R3791 T13244 T13238 punct ),clones
R3792 T13245 T13235 cc and,cells
R3793 T13246 T13247 amod heterozygous,cells
R3794 T13247 T13235 conj cells,cells
R3795 T13248 T13247 compound ES,cells
R3796 T13249 T13250 punct (,-
R3797 T13250 T13247 punct -,cells
R3798 T13251 T13250 punct +,-
R3799 T13252 T13250 punct /,-
R3800 T13253 T13250 punct ),-
R3801 T13254 T13209 punct .,analyses
R3802 T13256 T13257 compound Northern,blot
R3803 T13257 T13258 nsubjpass blot,performed
R3804 T13259 T13258 auxpass was,performed
R3805 T13260 T13261 mark as,described
R3806 T13261 T13258 advcl described,performed
R3807 T13262 T13261 advmod previously,described
R3808 T13263 T13264 punct [,20
R3809 T13264 T13261 parataxis 20,described
R3810 T13265 T13264 punct ],20
R3811 T13266 T13258 punct .,performed
R3812 T13268 T13269 aux To,confirm
R3813 T13269 T13270 advcl confirm,shown
R3814 T13271 T13272 det the,loading
R3815 T13272 T13269 dobj loading,confirm
R3816 T13273 T13272 prep of,loading
R3817 T13274 T13275 amod equal,amounts
R3818 T13275 T13273 pobj amounts,of
R3819 T13276 T13275 prep of,amounts
R3820 T13277 T13276 pobj RNA,of
R3821 T13278 T13270 punct ", ",shown
R3822 T13279 T13280 compound ethidium,bromide
R3823 T13280 T13281 compound bromide,staining
R3824 T13281 T13270 nsubjpass staining,shown
R3825 T13282 T13281 prep of,staining
R3826 T13283 T13284 amod ribosomal,RNA
R3827 T13284 T13282 pobj RNA,of
R3828 T13285 T13270 auxpass is,shown
R3829 T13286 T13270 advmod also,shown
R3830 T13287 T13288 punct (,middle
R3831 T13288 T13270 parataxis middle,shown
R3832 T13289 T13288 punct ),middle
R3833 T13290 T13270 punct .,shown
R3834 T13588 T13587 prep of,Analyses
R3835 T13589 T13590 npadvmod ESG1,null
R3836 T13590 T13592 amod null,cells
R3837 T13591 T13590 punct -,null
R3838 T13592 T13588 pobj cells,of
R3839 T13593 T13592 compound ES,cells
R3840 T13594 T13587 punct .,Analyses
R3841 T13596 T13597 meta A,morphology
R3842 T13598 T13596 punct ),A
R3843 T13599 T13597 det The,morphology
R3844 T13600 T13597 prep of,morphology
R3845 T13601 T13602 npadvmod ESG1,null
R3846 T13602 T13604 amod null,colonies
R3847 T13603 T13602 punct -,null
R3848 T13604 T13600 pobj colonies,of
R3849 T13605 T13604 compound ES,colonies
R3850 T13606 T13604 compound cell,colonies
R3851 T13607 T13597 acl grown,morphology
R3852 T13608 T13607 prep on,grown
R3853 T13609 T13610 compound STO,cells
R3854 T13610 T13608 pobj cells,on
R3855 T13611 T13610 compound feeder,cells
R3856 T13612 T13597 punct .,morphology
R3857 T13614 T13615 meta B,curve
R3858 T13616 T13614 punct ),B
R3859 T13617 T13615 compound Growth,curve
R3860 T13618 T13615 prep of,curve
R3861 T13619 T13620 amod wild,type
R3862 T13620 T13622 nmod type,cells
R3863 T13621 T13620 punct -,type
R3864 T13622 T13618 pobj cells,of
R3865 T13623 T13620 punct (,type
R3866 T13624 T13620 appos WT,type
R3867 T13625 T13620 punct ),type
R3868 T13626 T13620 punct ", ",type
R3869 T13627 T13620 conj ESG1,type
R3870 T13628 T13627 punct -,ESG1
R3871 T13629 T13627 amod null,ESG1
R3872 T13630 T13627 punct (,ESG1
R3873 T13631 T13632 punct -,-
R3874 T13632 T13627 appos -,ESG1
R3875 T13633 T13632 punct /,-
R3876 T13634 T13627 punct ),ESG1
R3877 T13635 T13627 cc and,ESG1
R3878 T13636 T13627 conj heterozygous,ESG1
R3879 T13637 T13636 punct (,heterozygous
R3880 T13638 T13639 punct +,-
R3881 T13639 T13636 appos -,heterozygous
R3882 T13640 T13639 punct /,-
R3883 T13641 T13622 punct ),cells
R3884 T13642 T13622 compound ES,cells
R3885 T13643 T13615 punct .,curve
R3886 T13645 T13646 det Each,clone
R3887 T13646 T13647 nsubjpass clone,plated
R3888 T13648 T13649 punct (,cells
R3889 T13649 T13646 parataxis cells,clone
R3890 T13650 T13651 quantmod 1,104
R3891 T13651 T13649 nummod 104,cells
R3892 T13652 T13651 punct ×,104
R3893 T13653 T13654 punct /,well
R3894 T13654 T13649 prep well,cells
R3895 T13655 T13649 punct ),cells
R3896 T13656 T13647 auxpass was,plated
R3897 T13657 T13647 prep in,plated
R3898 T13658 T13659 nummod 24,well
R3899 T13659 T13661 compound well,plates
R3900 T13660 T13659 punct -,well
R3901 T13661 T13657 pobj plates,in
R3902 T13662 T13647 punct .,plated
R3903 T13664 T13665 compound Cell,numbers
R3904 T13665 T13666 nsubjpass numbers,determined
R3905 T13667 T13666 auxpass were,determined
R3906 T13668 T13666 prep with,determined
R3907 T13669 T13670 det a,counter
R3908 T13670 T13668 pobj counter,with
R3909 T13671 T13670 compound Coulter,counter
R3910 T13672 T13666 prep at,determined
R3911 T13673 T13674 nummod 2,days
R3912 T13674 T13672 pobj days,at
R3913 T13675 T13673 punct ", ",2
R3914 T13676 T13673 conj 4,2
R3915 T13677 T13676 punct ", ",4
R3916 T13678 T13676 cc and,4
R3917 T13679 T13676 conj 6,4
R3918 T13680 T13666 punct .,determined
R3919 T13682 T13683 nsubjpass Data,shown
R3920 T13684 T13682 prep of,Data
R3921 T13685 T13686 punct +,-
R3922 T13686 T13688 punct -,cells
R3923 T13687 T13686 punct /,-
R3924 T13688 T13684 pobj cells,of
R3925 T13689 T13686 cc and,-
R3926 T13690 T13691 punct -,-
R3927 T13691 T13686 conj -,-
R3928 T13692 T13691 punct /,-
R3929 T13693 T13683 auxpass are,shown
R3930 T13694 T13683 prep as,shown
R3931 T13695 T13694 pobj averages,as
R3932 T13696 T13695 cc and,averages
R3933 T13697 T13698 amod standard,deviations
R3934 T13698 T13695 conj deviations,averages
R3935 T13699 T13695 prep of,averages
R3936 T13700 T13701 nummod three,clones
R3937 T13701 T13699 pobj clones,of
R3938 T13702 T13701 amod independent,clones
R3939 T13703 T13683 punct .,shown
R3940 T13705 T13706 meta C,section
R3941 T13707 T13705 punct ),C
R3942 T13708 T13706 det A,section
R3943 T13709 T13706 prep of,section
R3944 T13710 T13709 pobj teratoma,of
R3945 T13711 T13710 acl derived,teratoma
R3946 T13712 T13711 prep from,derived
R3947 T13713 T13714 npadvmod ESG1,null
R3948 T13714 T13716 amod null,cells
R3949 T13715 T13714 punct -,null
R3950 T13716 T13712 pobj cells,from
R3951 T13717 T13716 compound ES,cells
R3952 T13718 T13719 punct (,staining
R3953 T13719 T13711 parataxis staining,derived
R3954 T13720 T13719 nmod hematoxylin,staining
R3955 T13721 T13720 cc &,hematoxylin
R3956 T13722 T13720 conj eosin,hematoxylin
R3957 T13723 T13719 punct ),staining
R3958 T13724 T13706 punct .,section
R3959 T13961 T13962 compound Gene,expression
R3960 T13962 T13963 compound expression,analyses
R3961 T13964 T13963 prep of,analyses
R3962 T13965 T13966 npadvmod ESG1,null
R3963 T13966 T13968 amod null,cells
R3964 T13967 T13966 punct -,null
R3965 T13968 T13964 pobj cells,of
R3966 T13969 T13968 compound ES,cells
R3967 T13970 T13963 punct .,analyses
R3968 T13972 T13973 meta A,analyses
R3969 T13974 T13972 punct ),A
R3970 T13975 T13973 compound DNA,analyses
R3971 T13976 T13973 compound microarray,analyses
R3972 T13977 T13973 punct .,analyses
R3973 T13979 T13980 amod Total,RNA
R3974 T13980 T13981 nsubjpass RNA,labeled
R3975 T13982 T13980 prep from,RNA
R3976 T13983 T13984 amod wild,type
R3977 T13984 T13986 compound type,cells
R3978 T13985 T13984 punct -,type
R3979 T13986 T13982 pobj cells,from
R3980 T13987 T13986 compound ES,cells
R3981 T13988 T13986 cc and,cells
R3982 T13989 T13990 npadvmod ESG1,null
R3983 T13990 T13992 amod null,cells
R3984 T13991 T13990 punct -,null
R3985 T13992 T13986 conj cells,cells
R3986 T13993 T13992 compound ES,cells
R3987 T13994 T13981 auxpass were,labeled
R3988 T13995 T13981 prep with,labeled
R3989 T13996 T13995 pobj Cy3,with
R3990 T13997 T13996 cc and,Cy3
R3991 T13998 T13996 conj Cy5,Cy3
R3992 T13999 T13981 punct ", ",labeled
R3993 T14000 T13981 advmod respectively,labeled
R3994 T14001 T13981 punct .,labeled
R3995 T14003 T14004 nsubjpass Samples,hybridized
R3996 T14005 T14004 auxpass were,hybridized
R3997 T14006 T14004 prep to,hybridized
R3998 T14007 T14008 nmod Agilent,Microarrays
R3999 T14008 T14006 pobj Microarrays,to
R4000 T14009 T14008 nmod Mouse,Microarrays
R4001 T14010 T14008 amod Developmental,Microarrays
R4002 T14011 T14004 punct .,hybridized
R4003 T14013 T14014 det The,averages
R4004 T14014 T14015 nsubjpass averages,shown
R4005 T14016 T14014 prep of,averages
R4006 T14017 T14018 nummod two,clones
R4007 T14018 T14016 pobj clones,of
R4008 T14019 T14018 amod independent,clones
R4009 T14020 T14015 auxpass are,shown
R4010 T14021 T14015 punct .,shown
R4011 T14023 T14024 meta B,analyses
R4012 T14025 T14023 punct ),B
R4013 T14026 T14027 compound Western,blot
R4014 T14027 T14024 compound blot,analyses
R4015 T14028 T14024 punct .,analyses
R4016 T14030 T14031 compound Cell,lysates
R4017 T14031 T14032 nsubjpass lysates,examined
R4018 T14033 T14031 prep from,lysates
R4019 T14034 T14035 nmod ESG1,cells
R4020 T14035 T14033 pobj cells,from
R4021 T14036 T14034 punct +,ESG1
R4022 T14037 T14034 punct /,ESG1
R4023 T14038 T14034 punct -,ESG1
R4024 T14039 T14034 cc and,ESG1
R4025 T14040 T14034 conj ESG1,ESG1
R4026 T14041 T14040 punct -,ESG1
R4027 T14042 T14040 punct /,ESG1
R4028 T14043 T14040 punct -,ESG1
R4029 T14044 T14032 auxpass were,examined
R4030 T14045 T14032 prep for,examined
R4031 T14046 T14045 pobj expression,for
R4032 T14047 T14046 prep of,expression
R4033 T14048 T14047 pobj ESG1,of
R4034 T14049 T14048 punct ", ",ESG1
R4035 T14050 T14048 conj Oct3,ESG1
R4036 T14051 T14050 punct /,Oct3
R4037 T14052 T14050 nummod 4,Oct3
R4038 T14053 T14050 cc and,Oct3
R4039 T14054 T14050 conj CDK4,Oct3
R4040 T14055 T14032 prep with,examined
R4041 T14056 T14055 pobj immunoblotting,with
R4042 T14057 T14032 punct .,examined
R828 T4123 T4124 compound ESG1,cDNA
R829 T4124 T4122 pobj cDNA,to
R830 T4125 T4119 punct ", ",produce
R831 T4126 T4119 nsubj they,produce
R832 T4127 T4119 aux could,produce
R833 T4128 T4119 neg not,produce
R834 T4129 T4130 amod functional,proteins
R835 T4130 T4119 dobj proteins,produce
R836 T4131 T4119 punct ", ",produce
R837 T4132 T4119 prep because,produce
R838 T4133 T4132 pcomp of,because
R839 T4134 T4135 amod critical,mutations
R840 T4135 T4132 pobj mutations,because
R841 T4136 T4119 punct .,produce
R842 T4138 T4139 det This,result
R843 T4139 T4140 nsubj result,suggests
R844 T4141 T4142 mark that,are
R845 T4142 T4140 ccomp are,suggests
R846 T4143 T4142 expl there,are
R847 T4144 T4145 det a,number
R848 T4145 T4142 attr number,are
R849 T4146 T4145 amod larger,number
R850 T4147 T4145 prep of,number
R851 T4148 T4149 amod intron-less,pseudogenes
R852 T4149 T4147 pobj pseudogenes,of
R853 T4150 T4151 mark than,anticipated
R854 T4151 T4145 advcl anticipated,number
R855 T4152 T4151 advmod previously,anticipated
R856 T4153 T4140 punct .,suggests
R857 T4155 T4156 nsubj Existence,is
R858 T4157 T4155 prep of,Existence
R859 T4158 T4159 amod multiple,retropseudogenes
R860 T4159 T4157 pobj retropseudogenes,of
R861 T4160 T4161 det a,hallmark
R1118 T4442 T4440 ccomp is,concluded
R1119 T4443 T4444 det this,sequence
R1120 T4444 T4442 nsubj sequence,is
R1121 T4445 T4446 det the,gene
R1122 T4446 T4442 attr gene,is
R1123 T4447 T4446 compound ESG1,gene
R1124 T4448 T4440 punct .,concluded
R1141 T4450 T4451 nsubj We,found
R1144 T4452 T4451 advmod also,found
R1145 T4453 T4454 mark that,contained
R1150 T4455 T4456 det the,clones
R1153 T4457 T4456 nummod two,clones
R1154 T4458 T4456 compound BAC,clones
R1375 T6488 T6485 punct ),geo
R1376 T6489 T6472 cc or,fusion
R1377 T6490 T6491 det the,gene
R1378 T6491 T6472 conj gene,fusion
R1379 T6492 T6493 npadvmod hygromycin,resistant
R1380 T6493 T6491 amod resistant,gene
R1381 T6494 T6495 punct (,HygR
R1382 T6495 T6491 parataxis HygR,gene
R1383 T6496 T6495 punct ),HygR
R1384 T6497 T6466 advcl using,replaced
R1385 T6498 T6499 nummod two,vectors
R1445 T6582 T6583 det t,an
R1446 T6588 T6587 compound ES,cells
R1447 T6589 T6586 punct ", ",introduced
R1448 T6590 T6586 nsubj we,introduced
R1449 T6591 T6592 det the,vector
R1450 T6592 T6586 dobj vector,introduced
R1451 T6593 T6594 compound β,geo
R1452 T6594 T6592 compound geo,vector
R1453 T6595 T6594 punct -,geo
R1454 T6596 T6586 prep into,introduced
R1455 T6597 T6598 npadvmod HygR,heterozygous
R1456 T6598 T6599 amod heterozygous,cells
R1457 T6599 T6596 pobj cells,into
R1458 T6600 T6599 compound ES,cells
R1459 T6601 T6586 punct .,introduced
R1460 T6603 T6604 prep Of,were
R1461 T6605 T6606 nummod 105,colonies
R1462 T6606 T6603 pobj colonies,Of
R1463 T6607 T6608 npadvmod G418,resistant
R1464 T6608 T6606 amod resistant,colonies
R1465 T6609 T6608 punct -,resistant
R1466 T6610 T6606 acl tested,colonies
R1467 T6611 T6604 punct ", ",were
R1468 T6612 T6604 nsubj 49,were
R1585 T6741 T6742 amod retinoic,treatment
R1586 T6742 T6731 conj treatment,removal
R1587 T6743 T6742 compound acid,treatment
R1588 T6744 T6745 punct (,shown
R1589 T6745 T6742 parataxis shown,treatment
R1590 T6746 T6745 neg not,shown
R1591 T6747 T6745 punct ),shown
R1592 T6748 T6727 punct .,differentiated
R1593 T6750 T6751 advmod When,transplanted
R1594 T6751 T6752 advcl transplanted,produced
R1595 T6753 T6751 prep into,transplanted
R1596 T6754 T6755 compound hind,flanks
R1597 T6755 T6753 pobj flanks,into
R1598 T6756 T6755 prep of,flanks
R1599 T6757 T6758 amod nude,mice
R1600 T6758 T6756 pobj mice,of
R1601 T6759 T6752 punct ", ",produced
R1602 T6760 T6761 det these,cells
R1603 T6761 T6752 nsubj cells,produced
R1604 T6762 T6752 dobj teratomas,produced
R1605 T6763 T6762 punct ", ",teratomas
R1606 T6764 T6762 appos tumors,teratomas
R1607 T6765 T6764 acl containing,tumors
R1608 T6766 T6765 dobj components,containing
R1609 T6767 T6766 prep of,components
R1610 T6768 T6769 det all,layers
R1611 T6769 T6767 pobj layers,of
R1612 T6770 T6769 nummod three,layers
R1613 T6771 T6769 compound germ,layers
R1637 T6804 T6803 nmod x,a
R1638 T6807 T6808 det the,profiles
R1639 T6808 T6806 dobj profiles,mined
R1640 T6809 T6808 compound gene,profiles
R1641 T6810 T6808 compound expression,profiles
R1642 T6811 T6808 prep of,profiles
R1643 T6812 T6813 nmod ESG1,cells
R1644 T6813 T6811 pobj cells,of
R1645 T6814 T6812 punct -,ESG1
R1646 T6815 T6812 punct /,ESG1
R1647 T6816 T6812 punct -,ESG1
R1648 T6817 T6813 compound ES,cells
R1649 T6818 T6806 advcl using,mined
R1650 T6819 T6820 npadvmod oligonucleotide,based
R1651 T6820 T6822 amod based,microarrays
R1652 T6821 T6820 punct -,based
R1783 T6964 T6963 cc or,directly
R1784 T6965 T6963 conj indirectly,directly
R1785 T6966 T6960 agent by,regulated
R1786 T6967 T6966 pobj ESG1,by
R1787 T6968 T6960 punct .,regulated
R1788 T6970 T6971 mark Since,contains
R1789 T6971 T6973 advcl contains,stabilize
R1790 T6972 T6971 nsubj ESG1,contains
R1791 T6974 T6975 det a,domain
R1792 T6975 T6971 dobj domain,contains
R1793 T6976 T6977 nmod KH,type
R1794 T6977 T6975 nmod type,domain
R1795 T6978 T6977 punct -,type
R1796 T6979 T6980 npadvmod RNA,binding
R1797 T6980 T6975 amod binding,domain
R1798 T6981 T6980 punct -,binding
R1799 T6982 T6973 punct ", ",stabilize
R1800 T6983 T6973 nsubj it,stabilize
R1801 T6984 T6973 aux may,stabilize
R1802 T6985 T6973 dobj mRNA,stabilize
R1803 T6986 T6985 prep of,mRNA
R1804 T6987 T6988 det these,genes
R1805 T6988 T6986 pobj genes,of
R1806 T6989 T6973 punct .,stabilize
R1807 T6991 T6992 advmod Further,studied
R1808 T6992 T6993 nsubjpass studied,required
R1809 T6994 T6993 auxpass are,required
R1810 T6995 T6996 aux to,clarify
R1811 T6996 T6993 advcl clarify,required
R1812 T6997 T6998 det this,possibility
R1813 T6998 T6996 dobj possibility,clarify
R1814 T6999 T6993 punct .,required
R1815 T7004 T7005 nmod gen,ra
R1816 T7006 T7005 nmod e,ra
R1817 T7012 T7011 punct -,ESG1
R1818 T7015 T7016 compound knockout,mice
R1819 T7017 T7014 punct ", ",injected
R1820 T7018 T7014 nsubj we,injected
R1821 T7019 T7020 nmod β,ESG1
R1822 T7020 T7024 nmod ESG1,clones
R1823 T7021 T7020 punct -,ESG1
R1824 T7022 T7020 nmod geo,ESG1
R1825 T7023 T7020 punct -,ESG1
R1826 T7024 T7014 dobj clones,injected
R1827 T7025 T7020 punct +,ESG1
R1828 T7026 T7020 punct /,ESG1
R1829 T7027 T7020 punct -,ESG1
R1830 T7028 T7024 compound ES,clones
R1831 T7029 T7024 compound cell,clones
R1832 T7030 T7014 prep into,injected
R1833 T7031 T7032 det the,blastocysts
R1834 T7032 T7030 pobj blastocysts,into
R1835 T7033 T7032 prep of,blastocysts
R1836 T7034 T7035 compound C57BL6,mice
R1837 T7035 T7033 pobj mice,of
R1838 T7036 T7014 punct .,injected
R1839 T7038 T7039 nsubj We,obtained
R1840 T7040 T7041 compound germline,transmission
R1841 T7041 T7039 dobj transmission,obtained
R1842 T7042 T7039 prep from,obtained
R1843 T7043 T7044 nummod three,clones
R1844 T7044 T7042 pobj clones,from
R1845 T7045 T7039 punct .,obtained
R1846 T7047 T7048 nsubj We,obtained
R1847 T7049 T7050 nmod ESG1,mice
R1848 T7050 T7048 dobj mice,obtained
R1849 T7051 T7049 punct -,ESG1
R1850 T7052 T7049 punct /,ESG1
R1851 T7053 T7049 punct -,ESG1
R1852 T7054 T7048 prep at,obtained
R1853 T7055 T7056 det the,ratios
R1854 T7056 T7054 pobj ratios,at
R1855 T7057 T7056 amod Mendelian,ratios
R1856 T7058 T7056 punct (,ratios