PMC:13917 / 7913-8645 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/13917","sourcedb":"PMC","sourceid":"13917","source_url":"http://www.ncbi.nlm.nih.gov/pmc/13917","text":"Construction of the transgene\nA fusion gene, consisting of 4.2 kb of the 5'-flanking promoter sequences, including the transcriptional start site, of the ovine BLG gene [16] and the temperature-sensitive variant of SV40 T-Ag (tsA58) coding sequences [11], was constructed.\nThe 4.2 kb Sall/EcoRV fragment of the BLG promoter was isolated from pBJ39 (provided by Dr CBA Whitelaw) and subcloned into pBluescript vector (Stratagene Europe, Amsterdam, The Netherlands). A 3.9 kb Bgll fragment was isolated from pUC tsA58 [11], blunt ended using T4 DNA polymerase and digested with BamHI, and a 2.7 kb fragment containing sequences encoding large and small T-Ag was purified. This fragment was fused to the EcoRV site of the BLG promoter.","divisions":[{"label":"Title","span":{"begin":0,"end":29}}],"tracks":[{"project":"Colil","denotations":[{"id":"T18","span":{"begin":517,"end":519},"obj":"1711218"},{"id":"T16","span":{"begin":170,"end":172},"obj":"1695012"},{"id":"T17","span":{"begin":251,"end":253},"obj":"1711218"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/"}],"attributes":[{"subj":"T18","pred":"source","obj":"Colil"},{"subj":"T16","pred":"source","obj":"Colil"},{"subj":"T17","pred":"source","obj":"Colil"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Colil","color":"#93bbec","default":true}]}]}}