PMC:13902 / 17512-18098 JSONTXT

{"project":"Colil","denotations":[{"id":"T49","span":{"begin":91,"end":92},"obj":"421218"},{"id":"T50","span":{"begin":93,"end":95},"obj":"8137276"},{"id":"T51","span":{"begin":297,"end":299},"obj":"8137276"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/"}],"text":"A standard protocol for induction of breast cancer in female Sprague-Dawley rats was used [4,18], with initiation induced by a single intravenous dose of NMU (50 mg/kg body weight) at 8 weeks of age. Retinoids and tamoxifen were incorporated into powdered laboratory chow as described previously [18] and fed ad libitum, beginning 1 week after injection with NMU. Rats were fed 9cRA (Kuraray Company, Osaka, Japan) at 120 mg/kg of diet, tamoxifen (Sigma Chemical Co, St Louis, MO, USA) at 1.0 mg/kg of diet, and 4-HPR (RW Johnson Pharmaceutical Research Institute) at 782 mg/kg of diet."}