> top > docs > PMC:138691 > spans > 765-14273 > annotations

PMC:138691 / 765-14273 JSONTXT

Annnotations TAB JSON ListView MergeView

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T333 6-11 PR_EXT:000004804 denotes BRCA2
T334 27-33 UBERON:0000310 denotes breast
T335 56-60 SO_EXT:0000704 denotes gene
T336 115-122 SO_EXT:sequence_cloning_process denotes cloning
T337 163-169 UBERON:0000310 denotes breast
T338 182-187 CL_GO_EXT:cell denotes Cells
T339 193-199 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T340 200-205 PR_EXT:000004804 denotes BRCA2
T341 206-213 CHEBI_PR_EXT:protein denotes protein
T342 236-241 CL_GO_EXT:cell denotes cells
T343 243-254 SO_EXT:0000704 denotes genetically
T344 285-296 GO_SO_EXT:chromosome denotes chromosomal
T345 297-311 GO_SO_EXT:sequence_rearrangement_entity_or_process denotes rearrangements
T346 323-331 SO_EXT:biological_sequence denotes sequence
T347 346-353 CHEBI_PR_EXT:protein denotes protein
T348 360-371 CHEBI_SO_EXT:amino_acid denotes amino acids
T349 439-456 SO_EXT:0000657 denotes repeated segments
T350 469-476 SO_EXT:sequence_repeat_unit_or_region denotes repeats
T351 499-506 CHEBI_PR_EXT:protein denotes protein
T352 523-532 SO_EXT:biological_conservation_process_or_quality denotes conserved
T353 539-548 NCBITaxon:40674 denotes mammalian
T354 549-558 SO_EXT:0000855 denotes orthologs
T775 675-697 GO_EXT:0000150 denotes homologous recombinase
T776 698-703 PR_EXT:000013672 denotes RAD51
T777 706-715 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T778 722-729 GO:0005634 denotes nuclear
T779 741-746 PR_EXT:000004803 denotes BRCA1
T780 816-821 PR_EXT:000004803 denotes BRCA1
T781 826-831 PR_EXT:000004804 denotes BRCA2
T782 848-863 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T783 848-884 GO:0000725 denotes recombinational repair of DNA damage
T784 874-877 CHEBI_SO_EXT:DNA denotes DNA
T785 886-891 PR_EXT:000004803 denotes BRCA1
T786 896-901 PR_EXT:000004804 denotes BRCA2
T787 916-923 GO:0005634 denotes nuclear
T788 940-947 GO:0051320 denotes S phase
T789 970-973 CHEBI_SO_EXT:DNA denotes DNA
T790 970-989 CHEBI_EXT:50902 denotes DNA damaging agents
T791 1034-1043 _FRAGMENT denotes repair of
T792 1068-1078 GO:0006281 denotes DNA damage
T793 1068-1071 CHEBI_SO_EXT:DNA denotes DNA
T794 1091-1095 CL_GO_EXT:cell denotes Cell
T795 1122-1127 PR_EXT:000004803 denotes BRCA1
T796 1131-1136 PR_EXT:000004804 denotes BRCA2
T797 1222-1226 CL_GO_EXT:cell denotes cell
T798 1246-1261 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T799 1246-1268 GO:0000725 denotes recombinational repair
T800 1289-1294 PR_EXT:000004804 denotes BRCA2
T801 1314-1319 PR_EXT:000013672 denotes RAD51
T802 1320-1331 GO_EXT:recombinase denotes recombinase
T803 1343-1350 CHEBI_PR_EXT:protein denotes protein
T804 1351-1358 CHEBI_PR_EXT:protein denotes protein
T805 1436-1441 PR_EXT:000004803 denotes BRCA1
T806 1446-1451 PR_EXT:000013672 denotes RAD51
T807 1526-1533 SO_EXT:sequence_repeat_unit_or_region denotes repeats
T808 1537-1542 PR_EXT:000004804 denotes BRCA2
T809 1570-1575 PR_EXT:000013672 denotes RAD51
T810 1589-1594 CL_GO_EXT:cell denotes Cells
T811 1603-1608 PR_EXT:000004803 denotes BRCA1
T812 1642-1649 GO:0005634 denotes nuclear
T813 1650-1655 PR_EXT:000013672 denotes RAD51
T814 1707-1715 CHEBI_PR_EXT:protein denotes proteins
T815 1737-1749 _FRAGMENT denotes formation of
T816 1762-1771 GO:0065003 denotes complexes
T817 1750-1761 GO_EXT:recombinase denotes recombinase
T818 1762-1771 GO:0032991 denotes complexes
T819 1788-1791 CHEBI_SO_EXT:DNA denotes DNA
T820 1817-1824 SO_EXT:0000704 denotes genetic
T821 1841-1854 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T822 1881-1886 PR_EXT:000004803 denotes Brca1
T823 1886-1887 SO_EXT:sequence_nullness_or_absence denotes -
T824 1888-1889 SO_EXT:sequence_nullness_or_absence denotes -
T825 1890-1899 UBERON:0000922 denotes embryonic
T826 1890-1904 _FRAGMENT denotes embryonic stem
T827 1910-1915 CL:0002322 denotes cells
T828 1906-1908 _FRAGMENT denotes ES
T829 1910-1915 CL_GO_EXT:cell denotes cells
T830 1933-1948 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T831 1933-1948 _FRAGMENT denotes recombinational
T832 1953-1959 GO:0000725 denotes repair
T833 1949-1959 GO:0006302 denotes DSB repair
T834 2019-2024 PR_EXT:000004803 denotes BRCA1
T835 2029-2034 PR_EXT:000004804 denotes BRCA2
T836 2094-2097 CHEBI_SO_EXT:DNA denotes DNA
T837 2124-2129 PR_EXT:000004803 denotes BRCA1
T838 2134-2139 PR_EXT:000004804 denotes BRCA2
T839 2143-2149 GO:0006281 denotes repair
T840 2161-2166 CL_GO_EXT:cell denotes cells
T841 2195-2200 PR_EXT:000004804 denotes BRCA2
T842 2207-2212 PR_EXT:000004803 denotes BRCA1
T843 2240-2255 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T844 2240-2262 GO:0000725 denotes recombinational repair
T1740 2453-2466 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1741 2516-2529 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1742 2557-2560 CHEBI_SO_EXT:DNA denotes DNA
T1743 2590-2597 SO_EXT:sequence_alteration_process denotes mutated
T1744 2602-2607 SO_EXT:0000704 denotes genes
T1745 2613-2620 SO_EXT:sequence_coding_function denotes encodes
T1746 2659-2666 CHEBI_PR_EXT:protein denotes protein
T1747 2693-2709 SO_EXT:0000061 denotes restriction site
T1748 2714-2720 PR_EXT:P03882 denotes I-SceI
T1749 2724-2729 NCBITaxon_EXT:yeast denotes yeast
T1750 2730-2736 SO_EXT:0000188 denotes intron
T1751 2737-2744 SO_EXT:sequence_coding_function denotes encoded
T1752 2745-2757 GO_EXT:0004519 denotes endonuclease
T1753 2769-2773 CHEBI_SO_EXT:base denotes base
T1754 2769-2778 SO_EXT:0000028 denotes base pair
T1755 2779-2795 SO_EXT:0000409 denotes recognition site
T1756 2807-2819 GO:0009294 denotes transfection
T1757 2826-2832 PR_EXT:P03882 denotes I-SceI
T1758 2833-2843 GO:0010467 denotes expression
T1759 2844-2850 SO_EXT:0000440 denotes vector
T1760 2899-2906 SO_EXT:sequence_alteration_process denotes mutated
T1761 2907-2911 SO_EXT:sequence_copy_entity denotes copy
T1762 2932-2935 CHEBI_SO_EXT:DNA denotes DNA
T1763 2961-2970 GO:0042148 denotes invade(s)
T1764 2986-2994 SO_EXT:biological_sequence denotes sequence
T1765 3009-3015 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1766 3020-3024 SO_EXT:sequence_copy_entity denotes copy
T1767 3039-3048 _FRAGMENT denotes repair of
T1768 3053-3056 GO:0006302 denotes DSB
T1769 3081-3085 SO_EXT:0000704 denotes gene
T1770 3081-3096 GO:0035822 denotes gene conversion
T1771 3133-3142 SO_EXT:engineered_biological_sequence denotes construct
T1772 3174-3180 GO:0006281 denotes repair
T1773 3228-3232 SO_EXT:sequence_copy_entity denotes copy
T1774 3295-3299 CL_GO_EXT:cell denotes cell
T1775 3336-3346 GO:0010467 denotes expression
T1776 3847-3860 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1777 3891-3896 CL_GO_EXT:cell denotes cells
T1778 3912-3917 PR_EXT:000004804 denotes BRCA2
T1779 3918-3925 CHEBI_PR_EXT:protein denotes protein
T1780 3960-3966 GO:0006281 denotes repair
T1781 3971-3977 PR_EXT:P03882 denotes I-SceI
T1782 4009-4022 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1783 4024-4034 GO:0010467 denotes Expression
T1784 4038-4044 PR_EXT:P03882 denotes I-SceI
T1785 4071-4076 CL_GO_EXT:cell denotes cells
T1786 4106-4119 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1787 4127-4132 NCBITaxon:9606 denotes human
T1788 4133-4143 UBERON:0001264 denotes pancreatic
T1789 4150-4154 CL_GO_EXT:cell denotes cell
T1790 4177-4182 CL_GO_EXT:cell denotes cells
T1791 4191-4199 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T1792 4203-4208 PR_EXT:000004804 denotes BRCA2
T1793 4228-4233 SO_EXT:sequence_coding_function denotes codes
T1794 4240-4247 CHEBI_PR_EXT:protein denotes protein
T1795 4248-4257 SO_EXT:sequence_truncation_process denotes truncated
T1796 4261-4271 CHEBI_SO_EXT:amino_acid denotes amino acid
T1797 4338-4344 PR_EXT:P03882 denotes I-SceI
T1798 4353-4366 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1799 4428-4433 PR_EXT:000004804 denotes BRCA2
T1800 4433-4434 SO_EXT:normal_or_wild_type_or_present denotes +
T1801 4436-4441 NCBITaxon:9606 denotes human
T1802 4448-4452 CL_GO_EXT:cell denotes cell
T1803 4525-4536 SO_EXT:0000704 denotes genetically
T1804 4550-4555 CL_GO_EXT:cell denotes cells
T1805 4572-4577 PR_EXT:000004804 denotes BRCA2
T1806 4694-4709 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T1807 4694-4716 GO:0000725 denotes recombinational repair
T1808 4756-4765 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T1809 4773-4778 PR_EXT:000004804 denotes BRCA2
T1810 4905-4920 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T1811 4905-4927 GO:0000725 denotes recombinational repair
T1812 4950-4955 CL_GO_EXT:cell denotes cells
T1813 4999-5004 PR_EXT:000004804 denotes BRCA2
T1814 5037-5050 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1815 5056-5061 NCBITaxon:10088 denotes mouse
T1816 5062-5069 CL:0002322 denotes ES cell
T1817 5065-5069 CL_GO_EXT:cell denotes cell
T1818 5080-5089 GO:0010467 denotes expresses
T1819 5095-5104 SO_EXT:sequence_truncation_process denotes truncated
T1820 5105-5110 PR_EXT:000004804 denotes BRCA2
T1821 5111-5118 CHEBI_PR_EXT:protein denotes protein
T1822 5167-5180 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1823 5206-5211 CL_GO_EXT:cell denotes cells
T1824 5212-5222 GO:0010467 denotes expressing
T1825 5235-5240 PR_EXT:000004804 denotes BRCA2
T1826 5416-5423 GO:0010467 denotes express
T1827 5436-5441 PR_EXT:000004804 denotes BRCA2
T1828 5442-5449 CHEBI_PR_EXT:protein denotes protein
T1829 5530-5540 GO:0010467 denotes expressing
T1830 5553-5558 PR_EXT:000004804 denotes BRCA2
T1831 5580-5592 SO_EXT:sequence_recombinant_entity denotes recombinants
T1832 5607-5617 GO:0010467 denotes expressing
T1833 5623-5632 SO_EXT:sequence_truncation_process denotes truncated
T1834 5633-5638 PR_EXT:000004804 denotes BRCA2
T1835 5838-5843 CL_GO_EXT:cell denotes cells
T1836 5863-5871 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T1837 5902-5917 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T1838 5902-5924 GO:0000725 denotes recombinational repair
T1839 5960-5965 NCBITaxon:9606 denotes human
T1840 5966-5970 CL_GO_EXT:cell denotes cell
T1841 5994-5999 PR_EXT:000004804 denotes BRCA2
T1842 6072-6085 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1843 6102-6107 PR_EXT:000004804 denotes BRCA2
T1844 6250-6255 NCBITaxon:9606 denotes human
T1845 6326-6331 PR_EXT:000004804 denotes Brca2
T1846 6340-6345 CL_GO_EXT:cell denotes cells
T1847 6350-6355 PR_EXT:000004804 denotes Brca2
T1848 6355-6356 SO_EXT:normal_or_wild_type_or_present denotes +
T1849 6357-6358 SO_EXT:normal_or_wild_type_or_present denotes +
T1850 6359-6367 CL:0002322 denotes ES cells
T1851 6362-6367 CL_GO_EXT:cell denotes cells
T1852 6415-6420 PR_EXT:000004804 denotes Brca2
T1853 6439-6443 SO_EXT:sequence_nullness denotes null
T1854 6444-6450 SO_EXT:0001023 denotes allele
T1855 6517-6522 PR_EXT:000004804 denotes BRCA2
T1856 6535-6548 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1857 6601-6606 PR_EXT:000004804 denotes BRCA2
T1858 6645-6660 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T1859 6645-6667 GO:0000725 denotes recombinational repair
T1860 6676-6681 NCBITaxon:9606 denotes human
T1861 6686-6691 NCBITaxon:10088 denotes mouse
T3190 6847-6869 GO_EXT:0000150 denotes homologous recombinase
T3191 6870-6875 PR_EXT:000013672 denotes RAD51
T3192 6882-6889 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T3193 6925-6932 SO_EXT:sequence_repeat_unit_or_region denotes repeats
T3194 6938-6943 NCBITaxon:9606 denotes human
T3195 6944-6949 PR_EXT:000004804 denotes BRCA2
T3196 6951-6961 GO:0010467 denotes Expression
T3197 6978-6984 SO_EXT:sequence_repeat_unit_or_region denotes repeat
T3198 7034-7043 CHEBI_EXT:35222 denotes inhibitor
T3199 7047-7050 CHEBI_SO_EXT:DNA denotes DNA
T3200 7047-7057 GO:0006281 denotes DNA repair
T3201 7110-7120 GO:0010467 denotes expression
T3202 7124-7134 SO_EXT:engineered_biological_sequence denotes constructs
T3203 7155-7161 SO_EXT:sequence_repeat_unit_or_region denotes repeat
T3204 7171-7176 CL_GO_EXT:cell denotes cells
T3205 7210-7215 CL_GO_EXT:cell denotes cells
T3206 7236-7240 GO:0000086 denotes G2/M
T3207 7293-7304 GO:0009294 denotes transfected
T3208 7305-7310 CL_GO_EXT:cell denotes cells
T3209 7326-7333 GO:0005634 denotes nuclear
T3210 7334-7339 PR_EXT:000013672 denotes RAD51
T3211 7472-7482 GO:0010467 denotes expression
T3212 7499-7505 SO_EXT:sequence_repeat_unit_or_region denotes repeat
T3213 7507-7510 CHEBI_SO_EXT:DNA denotes DNA
T3214 7511-7518 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T3215 7530-7538 CHEBI:10545 denotes electron
T3216 7588-7595 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T3217 7627-7632 PR_EXT:000013672 denotes RAD51
T3218 7650-7660 GO_EXT:oligomeric_macromolecular_complex denotes oligomeric
T3219 7674-7677 CHEBI_SO_EXT:DNA denotes DNA
T3220 7686-7693 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T3221 7727-7732 PR_EXT:000013672 denotes RAD51
T3222 7733-7736 CHEBI_SO_EXT:DNA denotes DNA
T3223 7811-7816 PR_EXT:000004804 denotes BRCA2
T3224 7817-7824 CHEBI_PR_EXT:protein denotes protein
T3225 7840-7858 GO_EXT:negative_regulator denotes negative regulator
T3226 7876-7881 PR_EXT:000013672 denotes RAD51
T3227 7882-7885 CHEBI_SO_EXT:DNA denotes DNA
T3228 8037-8042 PR_EXT:000004804 denotes BRCA2
T3229 8052-8057 PR_EXT:000013672 denotes RAD51
T3230 8068-8081 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T3231 8087-8099 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3232 8103-8108 PR_EXT:000013672 denotes RAD51
T3233 8133-8138 CL_GO_EXT:cell denotes cells
T3234 8149-8154 NCBITaxon:9606 denotes human
T3235 8253-8262 SO_EXT:sequence_truncation_process denotes truncated
T3236 8263-8268 PR_EXT:000004804 denotes BRCA2
T3237 8269-8276 CHEBI_PR_EXT:protein denotes protein
T3238 8277-8286 GO:0010467 denotes expressed
T3239 8298-8303 CL_GO_EXT:cell denotes cells
T3240 8311-8320 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T3241 8328-8337 GO:0005737 denotes cytoplasm
T3242 8373-8383 SO_EXT:sequence_truncation_entity_or_process denotes truncation
T3243 8384-8391 CHEBI_PR_EXT:protein denotes protein
T3244 8400-8407 GO:0005634 denotes nuclear
T3245 8400-8427 SO_EXT:0001528 denotes nuclear localization signal
T3246 8408-8420 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3247 8462-8467 CL_GO_EXT:cell denotes cells
T3248 8487-8494 GO:0005634 denotes nuclear
T3249 8495-8507 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3250 8511-8516 PR_EXT:000013672 denotes RAD51
T3251 8547-8552 PR_EXT:000013672 denotes RAD51
T3252 8565-8572 GO_EXT:biological_movement_or_translocation_process denotes carried
T3253 8580-8587 GO:0005634 denotes nucleus
T3254 8591-8598 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T3255 8599-8604 PR_EXT:000004804 denotes BRCA2
T3256 8626-8631 PR_EXT:000013672 denotes RAD51
T3257 8664-8669 PR_EXT:000004804 denotes BRCA2
T3258 8682-8691 GO_EXT:biological_movement_or_translocation_process denotes transport
T3259 8700-8712 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3260 8716-8721 PR_EXT:000004804 denotes BRCA2
T3261 8729-8738 GO:0005737 denotes cytoplasm
T3262 8743-8752 GO:0051235 denotes sequester
T3263 8753-8758 PR_EXT:000013672 denotes RAD51
T3264 8788-8797 GO_EXT:biological_movement_or_translocation_process denotes transport
T3265 8847-8859 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3266 8863-8868 PR_EXT:000013672 denotes RAD51
T3267 8903-8908 PR_EXT:000004804 denotes BRCA2
T3268 8954-8959 PR_EXT:000004804 denotes BRCA2
T3269 9008-9013 PR_EXT:000013672 denotes RAD51
T3270 9014-9019 PR_EXT:000004804 denotes BRCA2
T3271 9082-9087 PR_EXT:000004804 denotes BRCA2
T3272 9101-9114 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T3273 9141-9148 GO:0005634 denotes nuclear
T3274 9149-9160 GO_EXT:biological_mover_or_transporter denotes transporter
T3275 9164-9169 PR_EXT:000013672 denotes RAD51
T3276 9255-9260 PR_EXT:000013672 denotes RAD51
T3277 9261-9266 PR_EXT:000004804 denotes BRCA2
T3278 9291-9296 PR_EXT:000013672 denotes RAD51
T3279 9392-9397 PR_EXT:000004804 denotes BRCA2
T3280 9399-9404 PR_EXT:000013672 denotes RAD51
T3281 9456-9465 GO_EXT:biological_recruitment denotes recruited
T3282 9484-9490 GO:0006281 denotes repair
T3283 9484-9498 GO:1990391 denotes repair complex
T3284 9564-9569 PR_EXT:000013672 denotes RAD51
T3285 9583-9590 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T3286 9606-9623 CHEBI_SO_EXT:single_stranded_DNA denotes single-strand DNA
T3287 9625-9630 CHEBI_SO_EXT:single_stranded_DNA denotes ssDNA
T3288 9637-9654 CHEBI_SO_EXT:double_stranded_DNA denotes double-strand DNA
T3289 9656-9661 CHEBI_SO_EXT:double_stranded_DNA denotes dsDNA
T3290 9699-9709 GO:0065007 denotes regulatory
T3291 9727-9732 PR_EXT:000004804 denotes BRCA2
T3292 9759-9764 PR_EXT:000013672 denotes RAD51
T3293 9765-9770 CHEBI_SO_EXT:double_stranded_DNA denotes dsDNA
T3294 9795-9808 GO:0051235 denotes sequestration
T3295 9812-9817 PR_EXT:000013672 denotes RAD51
T3296 9889-9894 PR_EXT:000013672 denotes RAD51
T3297 9895-9900 CHEBI_SO_EXT:single_stranded_DNA denotes ssDNA
T3298 9947-9952 PR_EXT:000013672 denotes RAD51
T3299 9958-9965 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T3300 9984-9989 CHEBI_SO_EXT:single_stranded_DNA denotes ssDNA
T3301 10014-10017 CHEBI_SO_EXT:DNA denotes DNA
T3302 10014-10029 GO:0006260 denotes DNA replication
T3303 10031-10036 PR_EXT:000004804 denotes BRCA2
T3304 10054-10059 PR_EXT:000013672 denotes RAD51
T3305 10060-10073 _FRAGMENT denotes assembly into
T3306 10097-10106 GO:0065003 denotes complexes
T3307 10074-10089 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T3308 10074-10096 GO:0000725 denotes recombinational repair
T3309 10090-10106 GO:1990391 denotes repair complexes
T3310 10113-10132 GO_EXT:negative_regulation denotes negative regulatory
T3311 10161-10166 PR_EXT:000013672 denotes RAD51
T3312 10167-10172 PR_EXT:000004804 denotes BRCA2
T3313 10261-10266 PR_EXT:000013672 denotes RAD51
T3314 10345-10352 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T3315 10368-10373 PR_EXT:000013672 denotes RAD51
T3316 10374-10379 PR_EXT:000004804 denotes BRCA2
T3317 10428-10435 CHEBI_PR_EXT:protein denotes protein
T3318 10460-10465 PR_EXT:000013672 denotes RAD51
T3319 10466-10471 PR_EXT:000004804 denotes BRCA2
T3320 11552-11557 PR_EXT:000004804 denotes BRCA2
T3321 11571-11576 PR_EXT:000013672 denotes RAD51
T3322 11587-11602 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T3323 11587-11609 GO:0000725 denotes recombinational repair
T3324 11644-11649 PR_EXT:000013672 denotes RAD51
T3325 11686-11694 CHEBI_PR_EXT:protein denotes proteins
T3326 11714-11719 PR_EXT:000013672 denotes RAD51
T3327 11757-11762 PR_EXT:000013672 denotes RAD51
T3328 11775-11778 CHEBI_SO_EXT:DNA denotes DNA
T3329 11806-11811 PR_EXT:000013672 denotes RAD51
T3330 11812-11820 SO_EXT:0000854 denotes paralogs
T3331 11846-11851 PR_EXT:000017505 denotes XRCC2
T3332 11853-11858 PR_EXT:000017506 denotes XRCC3
T3333 11860-11866 PR_EXT:000013676 denotes RAD51B
T3334 11868-11874 PR_EXT:000013675 denotes RAD51C
T3372 12986-12994 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T3374 13098-13103 PR_EXT:000013672 denotes RAD51
T3375 13104-13109 PR_EXT:000004804 denotes BRCA2
T3376 13179-13184 PR_EXT:000004804 denotes BRCA2
T3377 13188-13203 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T3378 13188-13210 GO:0000725 denotes recombinational repair
T3379 13278-13283 PR_EXT:000004804 denotes BRCA2
T3380 13333-13338 PR_EXT:000004804 denotes BRCA2
T3335 11879-11885 PR_EXT:000013677 denotes RAD51D
T3336 11905-11912 GO:0032991 denotes complex
T3337 11936-11941 PR_EXT:000013672 denotes RAD51
T3338 11957-11962 PR_EXT:000004804 denotes BRCA2
T3339 11984-11989 PR_EXT:000013672 denotes RAD51
T3340 11990-11998 SO_EXT:0000854 denotes paralogs
T3341 12012-12017 PR_EXT:000013672 denotes RAD51
T3342 12098-12103 PR_EXT:000004804 denotes BRCA2
T3343 12104-12113 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T3344 12135-12142 GO:0005634 denotes nuclear
T3345 12153-12158 PR_EXT:000013672 denotes RAD51
T3346 12202-12207 PR_EXT:000004804 denotes BRCA2
T3347 12233-12244 _FRAGMENT denotes assembly of
T3348 12263-12270 GO:0065003 denotes complex
T3349 12249-12262 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T3350 12263-12270 GO:0032991 denotes complex
T3351 12354-12359 PR_EXT:000004804 denotes BRCA2
T3352 12360-12365 PR_EXT:000013672 denotes RAD51
T3353 12424-12429 PR_EXT:000004804 denotes BRCA2
T3354 12458-12463 PR_EXT:000013672 denotes RAD51
T3355 12521-12526 PR_EXT:000013672 denotes RAD51
T3356 12539-12542 CHEBI_SO_EXT:DNA denotes DNA
T3357 12569-12577 GO_EXT:reaction_or_response denotes response
T3358 12667-12672 PR_EXT:000004804 denotes BRCA2
T3359 12677-12682 PR_EXT:000013672 denotes RAD51
T3360 12690-12705 GO_SO_EXT:sequence_rearrangement_process denotes recombinational
T3361 12690-12712 GO:0000725 denotes recombinational repair
T3362 12787-12792 PR_EXT:000004804 denotes BRCA2
T3363 12807-12819 GO_EXT:biological_movement_or_translocation_process denotes transporting
T3364 12820-12825 PR_EXT:000013672 denotes RAD51
T3365 12833-12840 GO:0005634 denotes nucleus
T3366 12879-12886 GO:0005634 denotes nuclear
T3367 12879-12906 SO_EXT:0001528 denotes nuclear localization signal
T3368 12887-12899 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3369 12910-12915 PR_EXT:000013672 denotes RAD51
T3370 12936-12941 PR_EXT:000013672 denotes RAD51
T3371 12980-12985 PR_EXT:000004804 denotes BRCA2
T3373 13073-13078 PR_EXT:000004804 denotes BRCA2
R558 T792 T791 _lexicallyChainedTo DNA damage,repair of
R559 T816 T815 _lexicallyChainedTo complexes,formation of
R560 T827 T826 _lexicallyChainedTo cells,embryonic stem
R561 T827 T828 _lexicallyChainedTo cells,ES
R562 T832 T831 _lexicallyChainedTo repair,recombinational
R1259 T1768 T1767 _lexicallyChainedTo DSB,repair of
R2230 T3348 T3347 _lexicallyChainedTo complex,assembly of
R2232 T3306 T3305 _lexicallyChainedTo complexes,assembly into

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T218 6-11 PR:000004804 denotes BRCA2
T219 27-33 UBERON:0000310 denotes breast
T220 56-60 SO:0000704 denotes gene
T221 163-169 UBERON:0000310 denotes breast
T222 200-205 PR:000004804 denotes BRCA2
T223 243-254 SO:0000704 denotes genetically
T224 439-456 SO:0000657 denotes repeated segments
T225 539-548 NCBITaxon:40674 denotes mammalian
T226 549-558 SO:0000855 denotes orthologs
T392 698-703 PR:000013672 denotes RAD51
T393 722-729 GO:0005634 denotes nuclear
T394 741-746 PR:000004803 denotes BRCA1
T395 816-821 PR:000004803 denotes BRCA1
T396 826-831 PR:000004804 denotes BRCA2
T397 848-884 GO:0000725 denotes recombinational repair of DNA damage
T398 886-891 PR:000004803 denotes BRCA1
T399 896-901 PR:000004804 denotes BRCA2
T400 916-923 GO:0005634 denotes nuclear
T401 940-947 GO:0051320 denotes S phase
T402 970-989 CHEBI:50902 denotes DNA damaging agents
T403 1034-1043 _FRAGMENT denotes repair of
T404 1068-1078 GO:0006281 denotes DNA damage
T405 1122-1127 PR:000004803 denotes BRCA1
T406 1131-1136 PR:000004804 denotes BRCA2
T407 1246-1268 GO:0000725 denotes recombinational repair
T408 1289-1294 PR:000004804 denotes BRCA2
T409 1314-1319 PR:000013672 denotes RAD51
T410 1436-1441 PR:000004803 denotes BRCA1
T411 1446-1451 PR:000013672 denotes RAD51
T412 1537-1542 PR:000004804 denotes BRCA2
T413 1570-1575 PR:000013672 denotes RAD51
T414 1603-1608 PR:000004803 denotes BRCA1
T415 1642-1649 GO:0005634 denotes nuclear
T416 1650-1655 PR:000013672 denotes RAD51
T417 1737-1749 _FRAGMENT denotes formation of
T418 1762-1771 GO:0065003 denotes complexes
T419 1762-1771 GO:0032991 denotes complexes
T420 1817-1824 SO:0000704 denotes genetic
T421 1881-1886 PR:000004803 denotes Brca1
T422 1890-1899 UBERON:0000922 denotes embryonic
T423 1890-1904 _FRAGMENT denotes embryonic stem
T424 1910-1915 CL:0002322 denotes cells
T425 1906-1908 _FRAGMENT denotes ES
T426 1933-1948 _FRAGMENT denotes recombinational
T427 1953-1959 GO:0000725 denotes repair
T428 1949-1959 GO:0006302 denotes DSB repair
T429 2019-2024 PR:000004803 denotes BRCA1
T430 2029-2034 PR:000004804 denotes BRCA2
T431 2124-2129 PR:000004803 denotes BRCA1
T432 2134-2139 PR:000004804 denotes BRCA2
T433 2143-2149 GO:0006281 denotes repair
T434 2195-2200 PR:000004804 denotes BRCA2
T435 2207-2212 PR:000004803 denotes BRCA1
T436 2240-2262 GO:0000725 denotes recombinational repair
T941 2602-2607 SO:0000704 denotes genes
T942 2693-2709 SO:0000061 denotes restriction site
T943 2714-2720 PR:P03882 denotes I-SceI
T944 2730-2736 SO:0000188 denotes intron
T945 2769-2778 SO:0000028 denotes base pair
T946 2779-2795 SO:0000409 denotes recognition site
T947 2807-2819 GO:0009294 denotes transfection
T948 2826-2832 PR:P03882 denotes I-SceI
T949 2833-2843 GO:0010467 denotes expression
T950 2844-2850 SO:0000440 denotes vector
T951 2961-2970 GO:0042148 denotes invade(s)
T952 3039-3048 _FRAGMENT denotes repair of
T953 3053-3056 GO:0006302 denotes DSB
T954 3081-3085 SO:0000704 denotes gene
T955 3081-3096 GO:0035822 denotes gene conversion
T956 3174-3180 GO:0006281 denotes repair
T957 3336-3346 GO:0010467 denotes expression
T958 3912-3917 PR:000004804 denotes BRCA2
T959 3960-3966 GO:0006281 denotes repair
T960 3971-3977 PR:P03882 denotes I-SceI
T961 4024-4034 GO:0010467 denotes Expression
T962 4038-4044 PR:P03882 denotes I-SceI
T963 4127-4132 NCBITaxon:9606 denotes human
T964 4133-4143 UBERON:0001264 denotes pancreatic
T965 4203-4208 PR:000004804 denotes BRCA2
T966 4338-4344 PR:P03882 denotes I-SceI
T967 4428-4433 PR:000004804 denotes BRCA2
T968 4436-4441 NCBITaxon:9606 denotes human
T969 4525-4536 SO:0000704 denotes genetically
T970 4572-4577 PR:000004804 denotes BRCA2
T971 4694-4716 GO:0000725 denotes recombinational repair
T972 4773-4778 PR:000004804 denotes BRCA2
T973 4905-4927 GO:0000725 denotes recombinational repair
T974 4999-5004 PR:000004804 denotes BRCA2
T975 5056-5061 NCBITaxon:10088 denotes mouse
T976 5062-5069 CL:0002322 denotes ES cell
T977 5080-5089 GO:0010467 denotes expresses
T978 5105-5110 PR:000004804 denotes BRCA2
T979 5212-5222 GO:0010467 denotes expressing
T980 5235-5240 PR:000004804 denotes BRCA2
T981 5416-5423 GO:0010467 denotes express
T982 5436-5441 PR:000004804 denotes BRCA2
T983 5530-5540 GO:0010467 denotes expressing
T984 5553-5558 PR:000004804 denotes BRCA2
T985 5607-5617 GO:0010467 denotes expressing
T986 5633-5638 PR:000004804 denotes BRCA2
T987 5902-5924 GO:0000725 denotes recombinational repair
T988 5960-5965 NCBITaxon:9606 denotes human
T989 5994-5999 PR:000004804 denotes BRCA2
T990 6102-6107 PR:000004804 denotes BRCA2
T991 6250-6255 NCBITaxon:9606 denotes human
T992 6326-6331 PR:000004804 denotes Brca2
T993 6350-6355 PR:000004804 denotes Brca2
T994 6359-6367 CL:0002322 denotes ES cells
T995 6415-6420 PR:000004804 denotes Brca2
T996 6444-6450 SO:0001023 denotes allele
T997 6517-6522 PR:000004804 denotes BRCA2
T998 6601-6606 PR:000004804 denotes BRCA2
T999 6645-6667 GO:0000725 denotes recombinational repair
T1000 6676-6681 NCBITaxon:9606 denotes human
T1001 6686-6691 NCBITaxon:10088 denotes mouse
T2031 6870-6875 PR:000013672 denotes RAD51
T2032 6938-6943 NCBITaxon:9606 denotes human
T2033 6944-6949 PR:000004804 denotes BRCA2
T2034 6951-6961 GO:0010467 denotes Expression
T2035 7034-7043 CHEBI:35222 denotes inhibitor
T2036 7047-7057 GO:0006281 denotes DNA repair
T2037 7110-7120 GO:0010467 denotes expression
T2038 7236-7240 GO:0000086 denotes G2/M
T2039 7293-7304 GO:0009294 denotes transfected
T2040 7326-7333 GO:0005634 denotes nuclear
T2041 7334-7339 PR:000013672 denotes RAD51
T2042 7472-7482 GO:0010467 denotes expression
T2043 7530-7538 CHEBI:10545 denotes electron
T2044 7627-7632 PR:000013672 denotes RAD51
T2045 7727-7732 PR:000013672 denotes RAD51
T2046 7811-7816 PR:000004804 denotes BRCA2
T2047 7876-7881 PR:000013672 denotes RAD51
T2048 8037-8042 PR:000004804 denotes BRCA2
T2049 8052-8057 PR:000013672 denotes RAD51
T2050 8103-8108 PR:000013672 denotes RAD51
T2051 8149-8154 NCBITaxon:9606 denotes human
T2052 8263-8268 PR:000004804 denotes BRCA2
T2053 8277-8286 GO:0010467 denotes expressed
T2054 8328-8337 GO:0005737 denotes cytoplasm
T2055 8400-8407 GO:0005634 denotes nuclear
T2056 8400-8427 SO:0001528 denotes nuclear localization signal
T2057 8487-8494 GO:0005634 denotes nuclear
T2058 8511-8516 PR:000013672 denotes RAD51
T2059 8547-8552 PR:000013672 denotes RAD51
T2060 8580-8587 GO:0005634 denotes nucleus
T2061 8599-8604 PR:000004804 denotes BRCA2
T2062 8626-8631 PR:000013672 denotes RAD51
T2063 8664-8669 PR:000004804 denotes BRCA2
T2064 8716-8721 PR:000004804 denotes BRCA2
T2065 8729-8738 GO:0005737 denotes cytoplasm
T2066 8743-8752 GO:0051235 denotes sequester
T2067 8753-8758 PR:000013672 denotes RAD51
T2068 8863-8868 PR:000013672 denotes RAD51
T2069 8903-8908 PR:000004804 denotes BRCA2
T2070 8954-8959 PR:000004804 denotes BRCA2
T2071 9008-9013 PR:000013672 denotes RAD51
T2072 9014-9019 PR:000004804 denotes BRCA2
T2073 9082-9087 PR:000004804 denotes BRCA2
T2074 9141-9148 GO:0005634 denotes nuclear
T2075 9164-9169 PR:000013672 denotes RAD51
T2076 9255-9260 PR:000013672 denotes RAD51
T2077 9261-9266 PR:000004804 denotes BRCA2
T2078 9291-9296 PR:000013672 denotes RAD51
T2079 9392-9397 PR:000004804 denotes BRCA2
T2080 9399-9404 PR:000013672 denotes RAD51
T2081 9484-9490 GO:0006281 denotes repair
T2082 9484-9498 GO:1990391 denotes repair complex
T2083 9564-9569 PR:000013672 denotes RAD51
T2084 9699-9709 GO:0065007 denotes regulatory
T2085 9727-9732 PR:000004804 denotes BRCA2
T2086 9759-9764 PR:000013672 denotes RAD51
T2087 9795-9808 GO:0051235 denotes sequestration
T2088 9812-9817 PR:000013672 denotes RAD51
T2089 9889-9894 PR:000013672 denotes RAD51
T2090 9947-9952 PR:000013672 denotes RAD51
T2091 10014-10029 GO:0006260 denotes DNA replication
T2092 10031-10036 PR:000004804 denotes BRCA2
T2093 10054-10059 PR:000013672 denotes RAD51
T2094 10060-10073 _FRAGMENT denotes assembly into
T2095 10097-10106 GO:0065003 denotes complexes
T2096 10074-10096 GO:0000725 denotes recombinational repair
T2097 10090-10106 GO:1990391 denotes repair complexes
T2098 10161-10166 PR:000013672 denotes RAD51
T2099 10167-10172 PR:000004804 denotes BRCA2
T2100 10261-10266 PR:000013672 denotes RAD51
T2101 10368-10373 PR:000013672 denotes RAD51
T2102 10374-10379 PR:000004804 denotes BRCA2
T2103 10460-10465 PR:000013672 denotes RAD51
T2104 10466-10471 PR:000004804 denotes BRCA2
T2105 11552-11557 PR:000004804 denotes BRCA2
T2106 11571-11576 PR:000013672 denotes RAD51
T2107 11587-11609 GO:0000725 denotes recombinational repair
T2108 11644-11649 PR:000013672 denotes RAD51
T2109 11714-11719 PR:000013672 denotes RAD51
T2110 11757-11762 PR:000013672 denotes RAD51
T2111 11806-11811 PR:000013672 denotes RAD51
T2112 11812-11820 SO:0000854 denotes paralogs
T2113 11846-11851 PR:000017505 denotes XRCC2
T2114 11853-11858 PR:000017506 denotes XRCC3
T2115 11860-11866 PR:000013676 denotes RAD51B
T2116 11868-11874 PR:000013675 denotes RAD51C
T2117 11879-11885 PR:000013677 denotes RAD51D
T2118 11905-11912 GO:0032991 denotes complex
T2119 11936-11941 PR:000013672 denotes RAD51
T2120 11957-11962 PR:000004804 denotes BRCA2
T2121 11984-11989 PR:000013672 denotes RAD51
T2122 11990-11998 SO:0000854 denotes paralogs
T2123 12012-12017 PR:000013672 denotes RAD51
T2124 12098-12103 PR:000004804 denotes BRCA2
T2125 12135-12142 GO:0005634 denotes nuclear
T2126 12153-12158 PR:000013672 denotes RAD51
T2127 12202-12207 PR:000004804 denotes BRCA2
T2128 12233-12244 _FRAGMENT denotes assembly of
T2129 12263-12270 GO:0065003 denotes complex
T2130 12263-12270 GO:0032991 denotes complex
T2131 12354-12359 PR:000004804 denotes BRCA2
T2132 12360-12365 PR:000013672 denotes RAD51
T2133 12424-12429 PR:000004804 denotes BRCA2
T2134 12458-12463 PR:000013672 denotes RAD51
T2135 12521-12526 PR:000013672 denotes RAD51
T2136 12667-12672 PR:000004804 denotes BRCA2
T2137 12677-12682 PR:000013672 denotes RAD51
T2138 12690-12712 GO:0000725 denotes recombinational repair
T2139 12787-12792 PR:000004804 denotes BRCA2
T2140 12820-12825 PR:000013672 denotes RAD51
T2141 12833-12840 GO:0005634 denotes nucleus
T2142 12879-12886 GO:0005634 denotes nuclear
T2143 12879-12906 SO:0001528 denotes nuclear localization signal
T2144 12910-12915 PR:000013672 denotes RAD51
T2145 12936-12941 PR:000013672 denotes RAD51
T2146 12980-12985 PR:000004804 denotes BRCA2
T2147 13073-13078 PR:000004804 denotes BRCA2
T2148 13098-13103 PR:000013672 denotes RAD51
T2149 13104-13109 PR:000004804 denotes BRCA2
T2150 13179-13184 PR:000004804 denotes BRCA2
T2151 13188-13210 GO:0000725 denotes recombinational repair
T2152 13278-13283 PR:000004804 denotes BRCA2
T2153 13333-13338 PR:000004804 denotes BRCA2
R236 T404 T403 _lexicallyChainedTo DNA damage,repair of
R237 T418 T417 _lexicallyChainedTo complexes,formation of
R238 T424 T423 _lexicallyChainedTo cells,embryonic stem
R239 T424 T425 _lexicallyChainedTo cells,ES
R240 T427 T426 _lexicallyChainedTo repair,recombinational
R563 T953 T952 _lexicallyChainedTo DSB,repair of
R1260 T2095 T2094 _lexicallyChainedTo complexes,assembly into
R1261 T2129 T2128 _lexicallyChainedTo complex,assembly of

craft-sa-dev

Id Subject Object Predicate Lexical cue
T227 6-11 NN denotes BRCA2
T228 12-15 VBD denotes was
T229 16-19 DT denotes the
T230 56-60 NN denotes gene
T231 20-26 JJ denotes second
T232 27-33 NN denotes breast
T233 34-40 NN denotes cancer
T234 41-55 NN denotes susceptibility
T235 61-63 TO denotes to
T236 67-77 VBN denotes discovered
T237 64-66 VB denotes be
T238 77-79 , denotes ,
T239 79-82 CC denotes and
T240 83-86 VBD denotes was
T241 87-95 VBN denotes isolated
T242 96-103 IN denotes through
T243 104-114 JJ denotes positional
T244 115-122 NN denotes cloning
T245 123-128 VBG denotes using
T246 129-133 NNS denotes data
T247 134-138 IN denotes from
T248 139-147 NNS denotes families
T249 148-152 IN denotes with
T250 153-162 JJ denotes inherited
T251 170-176 NN denotes cancer
T252 163-169 NN denotes breast
T253 177-178 -LRB- denotes [
T254 178-179 CD denotes 4
T255 179-180 -RRB- denotes ]
T256 180-181 . denotes .
T257 181-318 sentence denotes Cells with mutant BRCA2 protein are, like many cancer cells, genetically unstable and accumulate gross chromosomal rearrangements [5,6].
T258 182-187 NNS denotes Cells
T259 214-217 VBP denotes are
T260 188-192 IN denotes with
T261 193-199 JJ denotes mutant
T262 206-213 NN denotes protein
T263 200-205 NN denotes BRCA2
T264 217-219 , denotes ,
T265 219-223 IN denotes like
T266 224-228 JJ denotes many
T267 236-241 NNS denotes cells
T268 229-235 NN denotes cancer
T269 241-243 , denotes ,
T270 243-254 RB denotes genetically
T271 255-263 JJ denotes unstable
T272 264-267 CC denotes and
T273 268-278 VBP denotes accumulate
T274 279-284 JJ denotes gross
T275 297-311 NNS denotes rearrangements
T276 285-296 JJ denotes chromosomal
T277 312-313 -LRB- denotes [
T278 315-316 CD denotes 6
T279 313-314 CD denotes 5
T280 314-315 , denotes ,
T281 316-317 -RRB- denotes ]
T282 317-318 . denotes .
T283 318-565 sentence denotes The sequence of this large protein (3418 amino acids) offers very little clue to its function, although there are eight repeated segments (termed BRC repeats) in the middle of the protein that are highly conserved among mammalian orthologs [7,8].
T284 319-322 DT denotes The
T285 323-331 NN denotes sequence
T286 373-379 VBZ denotes offers
T287 332-334 IN denotes of
T288 335-339 DT denotes this
T289 346-353 NN denotes protein
T290 340-345 JJ denotes large
T291 354-355 -LRB- denotes (
T292 355-359 CD denotes 3418
T293 366-371 NNS denotes acids
T294 360-365 NN denotes amino
T295 371-372 -RRB- denotes )
T296 380-384 RB denotes very
T297 392-396 NN denotes clue
T298 385-391 JJ denotes little
T299 397-399 IN denotes to
T300 400-403 PRP$ denotes its
T301 404-412 NN denotes function
T302 412-414 , denotes ,
T303 414-422 IN denotes although
T304 429-432 VBP denotes are
T305 423-428 EX denotes there
T306 433-438 CD denotes eight
T307 448-456 NNS denotes segments
T308 439-447 JJ denotes repeated
T309 457-458 -LRB- denotes (
T310 458-464 VBN denotes termed
T311 465-468 NN denotes BRC
T312 469-476 NNS denotes repeats
T313 476-477 -RRB- denotes )
T314 478-480 IN denotes in
T315 481-484 DT denotes the
T316 485-491 NN denotes middle
T317 492-494 IN denotes of
T318 495-498 DT denotes the
T319 499-506 NN denotes protein
T320 507-511 WDT denotes that
T321 512-515 VBP denotes are
T322 516-522 RB denotes highly
T323 523-532 VBN denotes conserved
T324 533-538 IN denotes among
T325 539-548 JJ denotes mammalian
T326 549-558 NNS denotes orthologs
T327 559-560 -LRB- denotes [
T328 562-563 CD denotes 8
T329 560-561 CD denotes 7
T330 561-562 , denotes ,
T331 563-564 -RRB- denotes ]
T332 564-565 . denotes .
T437 617-626 VBG denotes Following
T438 788-796 VBN denotes provided
T439 627-630 DT denotes the
T440 640-649 NN denotes discovery
T441 631-639 NN denotes landmark
T442 650-652 IN denotes by
T443 653-659 NNP denotes Scully
T444 660-662 FW denotes et
T445 663-665 FW denotes al
T446 666-670 IN denotes that
T447 704-715 VBZ denotes colocalizes
T448 671-674 DT denotes the
T449 698-703 NN denotes RAD51
T450 675-685 JJ denotes homologous
T451 686-697 NN denotes recombinase
T452 716-718 IN denotes at
T453 719-729 JJ denotes subnuclear
T454 730-735 NNS denotes sites
T455 736-740 IN denotes with
T456 741-746 NN denotes BRCA1
T457 747-748 -LRB- denotes [
T458 748-749 CD denotes 9
T459 749-750 -RRB- denotes ]
T460 750-752 , denotes ,
T461 752-753 DT denotes a
T462 754-760 NN denotes number
T463 761-763 IN denotes of
T464 764-774 JJ denotes additional
T465 775-782 NNS denotes results
T466 783-787 VBP denotes have
T467 797-805 NN denotes evidence
T468 806-810 IN denotes that
T469 836-844 VBN denotes involved
T470 811-815 CC denotes both
T471 816-821 NN denotes BRCA1
T472 822-825 CC denotes and
T473 826-831 NN denotes BRCA2
T474 832-835 VBP denotes are
T475 845-847 IN denotes in
T476 848-863 JJ denotes recombinational
T477 864-870 NN denotes repair
T478 871-873 IN denotes of
T479 874-877 NN denotes DNA
T480 878-884 NN denotes damage
T481 884-885 . denotes .
T482 885-1000 sentence denotes BRCA1 and BRCA2 form discrete nuclear foci during the S phase and after exposure to DNA damaging agents [9,10,11].
T483 886-891 NN denotes BRCA1
T484 902-906 VBP denotes form
T485 892-895 CC denotes and
T486 896-901 NN denotes BRCA2
T487 907-915 JJ denotes discrete
T488 924-928 NNS denotes foci
T489 916-923 JJ denotes nuclear
T490 929-935 IN denotes during
T491 936-939 DT denotes the
T492 942-947 NN denotes phase
T493 940-941 NN denotes S
T494 948-951 CC denotes and
T495 952-957 IN denotes after
T496 958-966 NN denotes exposure
T497 967-969 IN denotes to
T498 970-973 NN denotes DNA
T499 974-982 VBG denotes damaging
T500 983-989 NNS denotes agents
T501 990-991 -LRB- denotes [
T502 996-998 CD denotes 11
T503 991-992 CD denotes 9
T504 992-993 , denotes ,
T505 993-995 CD denotes 10
T506 995-996 , denotes ,
T507 998-999 -RRB- denotes ]
T508 999-1000 . denotes .
T509 1000-1090 sentence denotes These foci are probably sites of repair of spontaneous and induced DNA damage [12,13,14].
T510 1001-1006 DT denotes These
T511 1007-1011 NNS denotes foci
T512 1012-1015 VBP denotes are
T513 1016-1024 RB denotes probably
T514 1025-1030 NNS denotes sites
T515 1031-1033 IN denotes of
T516 1034-1040 NN denotes repair
T517 1041-1043 IN denotes of
T518 1044-1055 JJ denotes spontaneous
T519 1072-1078 NN denotes damage
T520 1056-1059 CC denotes and
T521 1060-1067 JJ denotes induced
T522 1068-1071 NN denotes DNA
T523 1079-1080 -LRB- denotes [
T524 1086-1088 CD denotes 14
T525 1080-1082 CD denotes 12
T526 1082-1083 , denotes ,
T527 1083-1085 CD denotes 13
T528 1085-1086 , denotes ,
T529 1088-1089 -RRB- denotes ]
T530 1089-1090 . denotes .
T531 1090-1288 sentence denotes Cell lines defective in either BRCA1 or BRCA2 are sensitive to damaging agents that form double-strand breaks (DSBs), as are other cell lines defective in recombinational repair (reviewed in [15]).
T532 1091-1095 NN denotes Cell
T533 1096-1101 NNS denotes lines
T534 1137-1140 VBP denotes are
T535 1102-1111 JJ denotes defective
T536 1112-1114 IN denotes in
T537 1115-1121 CC denotes either
T538 1122-1127 NN denotes BRCA1
T539 1128-1130 CC denotes or
T540 1131-1136 NN denotes BRCA2
T541 1141-1150 JJ denotes sensitive
T542 1151-1153 IN denotes to
T543 1154-1162 VBG denotes damaging
T544 1163-1169 NNS denotes agents
T545 1170-1174 WDT denotes that
T546 1175-1179 VBP denotes form
T547 1180-1186 JJ denotes double
T548 1187-1193 JJ denotes strand
T549 1186-1187 HYPH denotes -
T550 1194-1200 NNS denotes breaks
T551 1201-1202 -LRB- denotes (
T552 1202-1206 NNS denotes DSBs
T553 1206-1207 -RRB- denotes )
T554 1207-1209 , denotes ,
T555 1209-1211 IN denotes as
T556 1212-1215 VBP denotes are
T557 1216-1221 JJ denotes other
T558 1227-1232 NNS denotes lines
T559 1222-1226 NN denotes cell
T560 1233-1242 JJ denotes defective
T561 1243-1245 IN denotes in
T562 1246-1261 JJ denotes recombinational
T563 1262-1268 NN denotes repair
T564 1269-1270 -LRB- denotes (
T565 1270-1278 VBN denotes reviewed
T566 1279-1281 IN denotes in
T567 1282-1283 -LRB- denotes [
T568 1283-1285 CD denotes 15
T569 1285-1286 -RRB- denotes ]
T570 1286-1287 -RRB- denotes )
T571 1287-1288 . denotes .
T572 1288-1382 sentence denotes BRCA2 interacts with the RAD51 recombinase via direct protein-protein contacts [16,17,18,19].
T573 1289-1294 NN denotes BRCA2
T574 1295-1304 VBZ denotes interacts
T575 1305-1309 IN denotes with
T576 1310-1313 DT denotes the
T577 1320-1331 NN denotes recombinase
T578 1314-1319 NN denotes RAD51
T579 1332-1335 IN denotes via
T580 1336-1342 JJ denotes direct
T581 1359-1367 NNS denotes contacts
T582 1343-1350 NN denotes protein
T583 1351-1358 NN denotes protein
T584 1350-1351 HYPH denotes -
T585 1368-1369 -LRB- denotes [
T586 1378-1380 CD denotes 19
T587 1369-1371 CD denotes 16
T588 1371-1372 , denotes ,
T589 1372-1374 CD denotes 17
T590 1374-1375 , denotes ,
T591 1375-1377 CD denotes 18
T592 1377-1378 , denotes ,
T593 1380-1381 -RRB- denotes ]
T594 1381-1382 . denotes .
T595 1382-1517 sentence denotes Biochemical analysis also showed interaction between BRCA1 and RAD51, although these detected interactions may have been indirect [9].
T596 1383-1394 JJ denotes Biochemical
T597 1395-1403 NN denotes analysis
T598 1409-1415 VBD denotes showed
T599 1404-1408 RB denotes also
T600 1416-1427 NN denotes interaction
T601 1428-1435 IN denotes between
T602 1436-1441 NN denotes BRCA1
T603 1442-1445 CC denotes and
T604 1446-1451 NN denotes RAD51
T605 1451-1453 , denotes ,
T606 1453-1461 IN denotes although
T607 1499-1503 VBN denotes been
T608 1462-1467 DT denotes these
T609 1477-1489 NNS denotes interactions
T610 1468-1476 VBN denotes detected
T611 1490-1493 MD denotes may
T612 1494-1498 VB denotes have
T613 1504-1512 JJ denotes indirect
T614 1513-1514 -LRB- denotes [
T615 1514-1515 CD denotes 9
T616 1515-1516 -RRB- denotes ]
T617 1516-1517 . denotes .
T618 1517-1588 sentence denotes The BRC repeats of BRCA2 are responsible for direct RAD51 interaction.
T619 1518-1521 DT denotes The
T620 1526-1533 NNS denotes repeats
T621 1522-1525 NN denotes BRC
T622 1543-1546 VBP denotes are
T623 1534-1536 IN denotes of
T624 1537-1542 NN denotes BRCA2
T625 1547-1558 JJ denotes responsible
T626 1559-1562 IN denotes for
T627 1563-1569 JJ denotes direct
T628 1576-1587 NN denotes interaction
T629 1570-1575 NN denotes RAD51
T630 1587-1588 . denotes .
T631 1588-1807 sentence denotes Cells lacking BRCA1/2 fail to form damage-induced subnuclear RAD51 foci with normal efficiency, suggesting that these proteins are required for the formation of recombinase complexes at the sites of DNA damage [20,21].
T632 1589-1594 NNS denotes Cells
T633 1611-1615 VBP denotes fail
T634 1595-1602 VBG denotes lacking
T635 1603-1608 NN denotes BRCA1
T636 1609-1610 NN denotes 2
T637 1608-1609 HYPH denotes /
T638 1616-1618 TO denotes to
T639 1619-1623 VB denotes form
T640 1624-1630 NN denotes damage
T641 1631-1638 VBN denotes induced
T642 1630-1631 HYPH denotes -
T643 1650-1655 NN denotes RAD51
T644 1639-1649 JJ denotes subnuclear
T645 1656-1660 NNS denotes foci
T646 1661-1665 IN denotes with
T647 1666-1672 JJ denotes normal
T648 1673-1683 NN denotes efficiency
T649 1683-1685 , denotes ,
T650 1685-1695 VBG denotes suggesting
T651 1696-1700 IN denotes that
T652 1720-1728 VBN denotes required
T653 1701-1706 DT denotes these
T654 1707-1715 NN denotes proteins
T655 1716-1719 VBP denotes are
T656 1729-1732 IN denotes for
T657 1733-1736 DT denotes the
T658 1737-1746 NN denotes formation
T659 1747-1749 IN denotes of
T660 1750-1761 NN denotes recombinase
T661 1762-1771 NNS denotes complexes
T662 1772-1774 IN denotes at
T663 1775-1778 DT denotes the
T664 1779-1784 NNS denotes sites
T665 1785-1787 IN denotes of
T666 1788-1791 NN denotes DNA
T667 1792-1798 NN denotes damage
T668 1799-1800 -LRB- denotes [
T669 1803-1805 CD denotes 21
T670 1800-1802 CD denotes 20
T671 1802-1803 , denotes ,
T672 1805-1806 -RRB- denotes ]
T673 1806-1807 . denotes .
T674 1807-1968 sentence denotes Finally, genetic measurements of recombination frequency have shown that Brca1-/- embryonic stem (ES) cells are deficient in recombinational DSB repair [22,23].
T675 1808-1815 RB denotes Finally
T676 1870-1875 VBN denotes shown
T677 1815-1817 , denotes ,
T678 1817-1824 JJ denotes genetic
T679 1825-1837 NNS denotes measurements
T680 1838-1840 IN denotes of
T681 1841-1854 NN denotes recombination
T682 1855-1864 NN denotes frequency
T683 1865-1869 VBP denotes have
T684 1876-1880 IN denotes that
T685 1916-1919 VBP denotes are
T686 1881-1886 NN denotes Brca1
T687 1910-1915 NNS denotes cells
T688 1886-1887 SYM denotes -
T689 1887-1888 HYPH denotes /
T690 1888-1889 SYM denotes -
T691 1890-1899 JJ denotes embryonic
T692 1900-1904 NN denotes stem
T693 1905-1906 -LRB- denotes (
T694 1906-1908 NN denotes ES
T695 1908-1909 -RRB- denotes )
T696 1920-1929 JJ denotes deficient
T697 1930-1932 IN denotes in
T698 1933-1948 JJ denotes recombinational
T699 1953-1959 NN denotes repair
T700 1949-1952 NN denotes DSB
T701 1960-1961 -LRB- denotes [
T702 1964-1966 CD denotes 23
T703 1961-1963 CD denotes 22
T704 1963-1964 , denotes ,
T705 1966-1967 -RRB- denotes ]
T706 1967-1968 . denotes .
T707 1968-2263 sentence denotes The similarity between phenotypes associated with BRCA1 and BRCA2 deficiency, together with data showing a similar effect of DNA damage on distribution of BRCA1 and BRCA2 in repair-proficient cells, led to the hypothesis that BRCA2, like BRCA1, is required for efficient recombinational repair.
T708 1969-1972 DT denotes The
T709 1973-1983 NN denotes similarity
T710 2168-2171 VBD denotes led
T711 1984-1991 IN denotes between
T712 1992-2002 NNS denotes phenotypes
T713 2003-2013 VBN denotes associated
T714 2014-2018 IN denotes with
T715 2019-2024 NN denotes BRCA1
T716 2035-2045 NN denotes deficiency
T717 2025-2028 CC denotes and
T718 2029-2034 NN denotes BRCA2
T719 2045-2047 , denotes ,
T720 2047-2055 RB denotes together
T721 2056-2060 IN denotes with
T722 2061-2065 NNS denotes data
T723 2066-2073 VBG denotes showing
T724 2074-2075 DT denotes a
T725 2084-2090 NN denotes effect
T726 2076-2083 JJ denotes similar
T727 2091-2093 IN denotes of
T728 2094-2097 NN denotes DNA
T729 2098-2104 NN denotes damage
T730 2105-2107 IN denotes on
T731 2108-2120 NN denotes distribution
T732 2121-2123 IN denotes of
T733 2124-2129 NN denotes BRCA1
T734 2130-2133 CC denotes and
T735 2134-2139 NN denotes BRCA2
T736 2140-2142 IN denotes in
T737 2143-2149 NN denotes repair
T738 2150-2160 JJ denotes proficient
T739 2149-2150 HYPH denotes -
T740 2161-2166 NNS denotes cells
T741 2166-2168 , denotes ,
T742 2172-2174 IN denotes to
T743 2175-2178 DT denotes the
T744 2179-2189 NN denotes hypothesis
T745 2190-2194 IN denotes that
T746 2217-2225 VBN denotes required
T747 2195-2200 NN denotes BRCA2
T748 2200-2202 , denotes ,
T749 2202-2206 IN denotes like
T750 2207-2212 NN denotes BRCA1
T751 2212-2214 , denotes ,
T752 2214-2216 VBZ denotes is
T753 2226-2229 IN denotes for
T754 2230-2239 JJ denotes efficient
T755 2256-2262 NN denotes repair
T756 2240-2255 JJ denotes recombinational
T757 2262-2263 . denotes .
T758 2263-2344 sentence denotes The paper by Moynahan et al [24] provides important support for this hypothesis.
T759 2264-2267 DT denotes The
T760 2268-2273 NN denotes paper
T761 2297-2305 VBZ denotes provides
T762 2274-2276 IN denotes by
T763 2277-2285 NNP denotes Moynahan
T764 2286-2288 FW denotes et
T765 2289-2291 FW denotes al
T766 2292-2293 -LRB- denotes [
T767 2293-2295 CD denotes 24
T768 2295-2296 -RRB- denotes ]
T769 2306-2315 JJ denotes important
T770 2316-2323 NN denotes support
T771 2324-2327 IN denotes for
T772 2328-2332 DT denotes this
T773 2333-2343 NN denotes hypothesis
T774 2343-2344 . denotes .
T1002 2417-2423 NNP denotes Pierce
T1003 2435-2443 VBN denotes designed
T1004 2424-2426 FW denotes et
T1005 2427-2429 FW denotes al
T1006 2430-2434 VBP denotes have
T1007 2444-2445 DT denotes a
T1008 2446-2449 NN denotes set
T1009 2450-2452 IN denotes of
T1010 2453-2466 NN denotes recombination
T1011 2467-2477 NNS denotes substrates
T1012 2478-2481 IN denotes for
T1013 2482-2491 VBG denotes measuring
T1014 2492-2495 DT denotes the
T1015 2496-2501 NN denotes level
T1016 2502-2504 IN denotes of
T1017 2505-2515 JJ denotes homologous
T1018 2516-2529 NN denotes recombination
T1019 2530-2532 FW denotes in
T1020 2533-2537 FW denotes vivo
T1021 2538-2539 -LRB- denotes (
T1022 2539-2543 NN denotes Fig.
T1023 2544-2545 CD denotes 1
T1024 2545-2546 -RRB- denotes )
T1025 2547-2548 -LRB- denotes [
T1026 2548-2550 CD denotes 25
T1027 2550-2551 -RRB- denotes ]
T1028 2551-2552 . denotes .
T1029 2552-2796 sentence denotes The DNA substrate contains a pair of mutated GFP genes (GFP encodes the easily detected green fluorescent protein), one of which contains a restriction site for I-SceI, a yeast intron encoded endonuclease with an 18 base pair recognition site.
T1030 2553-2556 DT denotes The
T1031 2561-2570 NN denotes substrate
T1032 2557-2560 NN denotes DNA
T1033 2571-2579 VBZ denotes contains
T1034 2580-2581 DT denotes a
T1035 2582-2586 NN denotes pair
T1036 2587-2589 IN denotes of
T1037 2590-2597 VBN denotes mutated
T1038 2602-2607 NNS denotes genes
T1039 2598-2601 NN denotes GFP
T1040 2608-2609 -LRB- denotes (
T1041 2613-2620 VBZ denotes encodes
T1042 2609-2612 NN denotes GFP
T1043 2621-2624 DT denotes the
T1044 2659-2666 NN denotes protein
T1045 2625-2631 RB denotes easily
T1046 2632-2640 VBN denotes detected
T1047 2641-2646 JJ denotes green
T1048 2647-2658 JJ denotes fluorescent
T1049 2666-2667 -RRB- denotes )
T1050 2667-2669 , denotes ,
T1051 2669-2672 CD denotes one
T1052 2682-2690 VBZ denotes contains
T1053 2673-2675 IN denotes of
T1054 2676-2681 WDT denotes which
T1055 2691-2692 DT denotes a
T1056 2705-2709 NN denotes site
T1057 2693-2704 NN denotes restriction
T1058 2710-2713 IN denotes for
T1059 2714-2715 NN denotes I
T1060 2716-2720 NN denotes SceI
T1061 2715-2716 HYPH denotes -
T1062 2720-2722 , denotes ,
T1063 2722-2723 DT denotes a
T1064 2745-2757 NN denotes endonuclease
T1065 2724-2729 NN denotes yeast
T1066 2730-2736 NN denotes intron
T1067 2737-2744 VBN denotes encoded
T1068 2758-2762 IN denotes with
T1069 2763-2765 DT denotes an
T1070 2791-2795 NN denotes site
T1071 2766-2768 CD denotes 18
T1072 2774-2778 NN denotes pair
T1073 2769-2773 NN denotes base
T1074 2779-2790 NN denotes recognition
T1075 2795-2796 . denotes .
T1076 2796-2919 sentence denotes Transient transfection of an I-SceI expression vector results in the production of a DSB in the first mutated copy of GFP.
T1077 2797-2806 JJ denotes Transient
T1078 2807-2819 NN denotes transfection
T1079 2851-2858 VBZ denotes results
T1080 2820-2822 IN denotes of
T1081 2823-2825 DT denotes an
T1082 2844-2850 NN denotes vector
T1083 2826-2827 NN denotes I
T1084 2828-2832 NN denotes SceI
T1085 2827-2828 HYPH denotes -
T1086 2833-2843 NN denotes expression
T1087 2859-2861 IN denotes in
T1088 2862-2865 DT denotes the
T1089 2866-2876 NN denotes production
T1090 2877-2879 IN denotes of
T1091 2880-2881 DT denotes a
T1092 2882-2885 NN denotes DSB
T1093 2886-2888 IN denotes in
T1094 2889-2892 DT denotes the
T1095 2907-2911 NN denotes copy
T1096 2893-2898 JJ denotes first
T1097 2899-2906 VBN denotes mutated
T1098 2912-2914 IN denotes of
T1099 2915-2918 NN denotes GFP
T1100 2918-2919 . denotes .
T1101 2919-3103 sentence denotes One or both DNA ends formed by the break invade(s) the homologous sequence in the second mutant GFP copy, resulting in repair of the DSB via a homology-mediated gene conversion event.
T1102 2920-2923 CD denotes One
T1103 2961-2967 VB denotes invade
T1104 2924-2926 CC denotes or
T1105 2927-2931 CC denotes both
T1106 2936-2940 NNS denotes ends
T1107 2932-2935 NN denotes DNA
T1108 2941-2947 VBN denotes formed
T1109 2948-2950 IN denotes by
T1110 2951-2954 DT denotes the
T1111 2955-2960 NN denotes break
T1112 2967-2968 -LRB- denotes (
T1113 2968-2969 VBZ denotes s
T1114 2969-2970 -RRB- denotes )
T1115 2971-2974 DT denotes the
T1116 2986-2994 NN denotes sequence
T1117 2975-2985 JJ denotes homologous
T1118 2995-2997 IN denotes in
T1119 2998-3001 DT denotes the
T1120 3020-3024 NN denotes copy
T1121 3002-3008 JJ denotes second
T1122 3009-3015 JJ denotes mutant
T1123 3016-3019 NN denotes GFP
T1124 3024-3026 , denotes ,
T1125 3026-3035 VBG denotes resulting
T1126 3036-3038 IN denotes in
T1127 3039-3045 NN denotes repair
T1128 3046-3048 IN denotes of
T1129 3049-3052 DT denotes the
T1130 3053-3056 NN denotes DSB
T1131 3057-3060 IN denotes via
T1132 3061-3062 DT denotes a
T1133 3097-3102 NN denotes event
T1134 3063-3071 NN denotes homology
T1135 3072-3080 VBN denotes mediated
T1136 3071-3072 HYPH denotes -
T1137 3081-3085 NN denotes gene
T1138 3086-3096 NN denotes conversion
T1139 3102-3103 . denotes .
T1140 3103-3240 sentence denotes The configuration of the GFP construct is such that homology-mediated repair often results in the formation of a functional copy of GFP.
T1141 3104-3107 DT denotes The
T1142 3108-3121 NN denotes configuration
T1143 3143-3145 VBZ denotes is
T1144 3122-3124 IN denotes of
T1145 3125-3128 DT denotes the
T1146 3133-3142 NN denotes construct
T1147 3129-3132 NN denotes GFP
T1148 3146-3150 JJ denotes such
T1149 3151-3155 IN denotes that
T1150 3187-3194 VBZ denotes results
T1151 3156-3164 NN denotes homology
T1152 3165-3173 VBN denotes mediated
T1153 3164-3165 HYPH denotes -
T1154 3174-3180 NN denotes repair
T1155 3181-3186 RB denotes often
T1156 3195-3197 IN denotes in
T1157 3198-3201 DT denotes the
T1158 3202-3211 NN denotes formation
T1159 3212-3214 IN denotes of
T1160 3215-3216 DT denotes a
T1161 3228-3232 NN denotes copy
T1162 3217-3227 JJ denotes functional
T1163 3233-3235 IN denotes of
T1164 3236-3239 NN denotes GFP
T1165 3239-3240 . denotes .
T1166 3240-3354 sentence denotes Such events can be detected by fluorescence-activated cell sorting analysis by virtue of their expression of GFP.
T1167 3241-3245 JJ denotes Such
T1168 3246-3252 NNS denotes events
T1169 3260-3268 VBN denotes detected
T1170 3253-3256 MD denotes can
T1171 3257-3259 VB denotes be
T1172 3269-3271 IN denotes by
T1173 3272-3284 NN denotes fluorescence
T1174 3285-3294 VBN denotes activated
T1175 3284-3285 HYPH denotes -
T1176 3300-3307 NN denotes sorting
T1177 3295-3299 NN denotes cell
T1178 3308-3316 NN denotes analysis
T1179 3317-3319 IN denotes by
T1180 3320-3326 NN denotes virtue
T1181 3327-3329 IN denotes of
T1182 3330-3335 PRP$ denotes their
T1183 3336-3346 NN denotes expression
T1184 3347-3349 IN denotes of
T1185 3350-3353 NN denotes GFP
T1186 3353-3354 . denotes .
T1187 3354-4023 sentence denotes Figure 1 Recombination substrates used for assaying homology-directed repair. Cutting at the I-SceI site within the mutant GFP (SceGFP) results in a double-strand break that can be repaired through homologous gene conversion using a 3'-truncated copy of GFP as sequence donor. The mechanism results in the formation of a functional copy of the GFP gene. The model shown assumes gene conversion occurs via the synthesis-dependent annealing mechanism. Moynahan et al [24] have used such a GFP recombination substrate to demonstrate that cells with defective BRCA2 protein are deficient in their ability to repair the I-SceI-induced DSB through homologous recombination.
T1188 3806-3814 NNP denotes Moynahan
T1189 3831-3835 VBN denotes used
T1190 3815-3817 FW denotes et
T1191 3818-3820 FW denotes al
T1192 3821-3822 -LRB- denotes [
T1193 3822-3824 CD denotes 24
T1194 3824-3825 -RRB- denotes ]
T1195 3826-3830 VBP denotes have
T1196 3836-3840 PDT denotes such
T1197 3861-3870 NN denotes substrate
T1198 3841-3842 DT denotes a
T1199 3843-3846 NN denotes GFP
T1200 3847-3860 NN denotes recombination
T1201 3871-3873 TO denotes to
T1202 3874-3885 VB denotes demonstrate
T1203 3886-3890 IN denotes that
T1204 3926-3929 VBP denotes are
T1205 3891-3896 NNS denotes cells
T1206 3897-3901 IN denotes with
T1207 3902-3911 JJ denotes defective
T1208 3918-3925 NN denotes protein
T1209 3912-3917 NN denotes BRCA2
T1210 3930-3939 JJ denotes deficient
T1211 3940-3942 IN denotes in
T1212 3943-3948 PRP$ denotes their
T1213 3949-3956 NN denotes ability
T1214 3957-3959 TO denotes to
T1215 3960-3966 VB denotes repair
T1216 3967-3970 DT denotes the
T1217 3986-3989 NN denotes DSB
T1218 3971-3972 NN denotes I
T1219 3973-3977 NN denotes SceI
T1220 3972-3973 HYPH denotes -
T1221 3978-3985 VBN denotes induced
T1222 3977-3978 HYPH denotes -
T1223 3990-3997 IN denotes through
T1224 3998-4008 JJ denotes homologous
T1225 4009-4022 NN denotes recombination
T1226 4022-4023 . denotes .
T1227 4023-4168 sentence denotes Expression of I-SceI resulted in 1 out of 1400 cells producing GFP via homologous recombination in the human pancreatic tumor cell line CAPAN-1.
T1228 4024-4034 NN denotes Expression
T1229 4045-4053 VBD denotes resulted
T1230 4035-4037 IN denotes of
T1231 4038-4039 NN denotes I
T1232 4040-4044 NN denotes SceI
T1233 4039-4040 HYPH denotes -
T1234 4054-4056 IN denotes in
T1235 4057-4058 CD denotes 1
T1236 4066-4070 CD denotes 1400
T1237 4059-4062 IN denotes out
T1238 4063-4065 IN denotes of
T1239 4071-4076 NNS denotes cells
T1240 4077-4086 VBG denotes producing
T1241 4087-4090 NN denotes GFP
T1242 4091-4094 IN denotes via
T1243 4095-4105 JJ denotes homologous
T1244 4106-4119 NN denotes recombination
T1245 4120-4122 IN denotes in
T1246 4123-4126 DT denotes the
T1247 4155-4159 NN denotes line
T1248 4127-4132 JJ denotes human
T1249 4150-4154 NN denotes cell
T1250 4133-4143 JJ denotes pancreatic
T1251 4144-4149 NN denotes tumor
T1252 4160-4165 NN denotes CAPAN
T1253 4165-4166 HYPH denotes -
T1254 4166-4167 CD denotes 1
T1255 4167-4168 . denotes .
T1256 4168-4298 sentence denotes CAPAN-1 cells carry a deletion of BRCA2 on one homolog and codes for a protein truncated at amino acid 1981 on the other homolog.
T1257 4169-4174 NN denotes CAPAN
T1258 4177-4182 NNS denotes cells
T1259 4174-4175 HYPH denotes -
T1260 4175-4176 CD denotes 1
T1261 4183-4188 VBP denotes carry
T1262 4189-4190 DT denotes a
T1263 4191-4199 NN denotes deletion
T1264 4200-4202 IN denotes of
T1265 4203-4208 NN denotes BRCA2
T1266 4209-4211 IN denotes on
T1267 4212-4215 CD denotes one
T1268 4216-4223 NN denotes homolog
T1269 4224-4227 CC denotes and
T1270 4228-4233 NNS denotes codes
T1271 4234-4237 IN denotes for
T1272 4238-4239 DT denotes a
T1273 4240-4247 NN denotes protein
T1274 4248-4257 VBN denotes truncated
T1275 4258-4260 IN denotes at
T1276 4261-4266 JJ denotes amino
T1277 4267-4271 NN denotes acid
T1278 4272-4276 CD denotes 1981
T1279 4277-4279 IN denotes on
T1280 4280-4283 DT denotes the
T1281 4290-4297 NN denotes homolog
T1282 4284-4289 JJ denotes other
T1283 4297-4298 . denotes .
T1284 4298-4459 sentence denotes The authors indicate that the level of I-SceI-induced recombination in CAPAN-1 is over 100-fold less than that seen using other (BRCA2+) human tumor cell lines.
T1285 4299-4302 DT denotes The
T1286 4303-4310 NNS denotes authors
T1287 4311-4319 VBP denotes indicate
T1288 4320-4324 IN denotes that
T1289 4378-4380 VBZ denotes is
T1290 4325-4328 DT denotes the
T1291 4329-4334 NN denotes level
T1292 4335-4337 IN denotes of
T1293 4338-4339 NN denotes I
T1294 4340-4344 NN denotes SceI
T1295 4339-4340 HYPH denotes -
T1296 4345-4352 VBN denotes induced
T1297 4344-4345 HYPH denotes -
T1298 4353-4366 NN denotes recombination
T1299 4367-4369 IN denotes in
T1300 4370-4375 NN denotes CAPAN
T1301 4375-4376 HYPH denotes -
T1302 4376-4377 CD denotes 1
T1303 4381-4385 IN denotes over
T1304 4386-4394 RB denotes 100-fold
T1305 4395-4399 JJR denotes less
T1306 4400-4404 IN denotes than
T1307 4405-4409 DT denotes that
T1308 4410-4414 VBN denotes seen
T1309 4415-4420 VBG denotes using
T1310 4421-4426 JJ denotes other
T1311 4428-4434 JJ denotes BRCA2+
T1312 4427-4428 -LRB- denotes (
T1313 4453-4458 NNS denotes lines
T1314 4434-4435 -RRB- denotes )
T1315 4436-4441 JJ denotes human
T1316 4442-4447 NN denotes tumor
T1317 4448-4452 NN denotes cell
T1318 4458-4459 . denotes .
T1319 4459-4636 sentence denotes The different lines examined, however, are very likely to differ genetically from CAPAN-1 cells not only at the BRCA2 locus, but also at a very large number of additional loci.
T1320 4460-4463 DT denotes The
T1321 4474-4479 NNS denotes lines
T1322 4464-4473 JJ denotes different
T1323 4499-4502 VBP denotes are
T1324 4480-4488 VBN denotes examined
T1325 4488-4490 , denotes ,
T1326 4490-4497 RB denotes however
T1327 4497-4499 , denotes ,
T1328 4503-4507 RB denotes very
T1329 4508-4514 JJ denotes likely
T1330 4515-4517 TO denotes to
T1331 4518-4524 VB denotes differ
T1332 4525-4536 RB denotes genetically
T1333 4537-4541 IN denotes from
T1334 4542-4547 NN denotes CAPAN
T1335 4550-4555 NNS denotes cells
T1336 4547-4548 HYPH denotes -
T1337 4548-4549 CD denotes 1
T1338 4556-4559 RB denotes not
T1339 4565-4567 IN denotes at
T1340 4560-4564 RB denotes only
T1341 4568-4571 DT denotes the
T1342 4578-4583 NN denotes locus
T1343 4572-4577 NN denotes BRCA2
T1344 4583-4585 , denotes ,
T1345 4585-4588 CC denotes but
T1346 4589-4593 RB denotes also
T1347 4594-4596 IN denotes at
T1348 4597-4598 DT denotes a
T1349 4610-4616 NN denotes number
T1350 4599-4603 RB denotes very
T1351 4604-4609 JJ denotes large
T1352 4617-4619 IN denotes of
T1353 4620-4630 JJ denotes additional
T1354 4631-4635 NNS denotes loci
T1355 4635-4636 . denotes .
T1356 4636-4784 sentence denotes This raises the possibility that some or even all of the recombinational repair defect seen in CAPAN-1 could be due to mutations at non-BRCA2 loci.
T1357 4637-4641 DT denotes This
T1358 4642-4648 VBZ denotes raises
T1359 4649-4652 DT denotes the
T1360 4653-4664 NN denotes possibility
T1361 4665-4669 IN denotes that
T1362 4746-4748 VB denotes be
T1363 4670-4674 DT denotes some
T1364 4675-4677 CC denotes or
T1365 4678-4682 RB denotes even
T1366 4683-4686 DT denotes all
T1367 4687-4689 IN denotes of
T1368 4690-4693 DT denotes the
T1369 4717-4723 NN denotes defect
T1370 4694-4709 JJ denotes recombinational
T1371 4710-4716 NN denotes repair
T1372 4724-4728 VBN denotes seen
T1373 4729-4731 IN denotes in
T1374 4732-4737 NN denotes CAPAN
T1375 4737-4738 HYPH denotes -
T1376 4738-4739 CD denotes 1
T1377 4740-4745 MD denotes could
T1378 4749-4752 IN denotes due
T1379 4753-4755 IN denotes to
T1380 4756-4765 NNS denotes mutations
T1381 4766-4768 IN denotes at
T1382 4769-4778 JJ denotes non-BRCA2
T1383 4779-4783 NNS denotes loci
T1384 4783-4784 . denotes .
T1385 4784-5005 sentence denotes While Moynahan et al were careful to point out this problem, two sets of results argue against the possibility that the recombinational repair deficiency of CAPAN-1 cells is completely independent of its defect in BRCA2.
T1386 4785-4790 IN denotes While
T1387 4806-4810 VBD denotes were
T1388 4791-4799 NNP denotes Moynahan
T1389 4800-4802 FW denotes et
T1390 4803-4805 FW denotes al
T1391 4866-4871 VBP denotes argue
T1392 4811-4818 JJ denotes careful
T1393 4819-4821 TO denotes to
T1394 4822-4827 VB denotes point
T1395 4828-4831 RP denotes out
T1396 4832-4836 DT denotes this
T1397 4837-4844 NN denotes problem
T1398 4844-4846 , denotes ,
T1399 4846-4849 CD denotes two
T1400 4850-4854 NNS denotes sets
T1401 4855-4857 IN denotes of
T1402 4858-4865 NNS denotes results
T1403 4872-4879 IN denotes against
T1404 4880-4883 DT denotes the
T1405 4884-4895 NN denotes possibility
T1406 4896-4900 IN denotes that
T1407 4956-4958 VBZ denotes is
T1408 4901-4904 DT denotes the
T1409 4928-4938 NN denotes deficiency
T1410 4905-4920 JJ denotes recombinational
T1411 4921-4927 NN denotes repair
T1412 4939-4941 IN denotes of
T1413 4942-4947 NN denotes CAPAN
T1414 4950-4955 NNS denotes cells
T1415 4947-4948 HYPH denotes -
T1416 4948-4949 CD denotes 1
T1417 4959-4969 RB denotes completely
T1418 4970-4981 JJ denotes independent
T1419 4982-4984 IN denotes of
T1420 4985-4988 PRP$ denotes its
T1421 4989-4995 NN denotes defect
T1422 4996-4998 IN denotes in
T1423 4999-5004 NN denotes BRCA2
T1424 5004-5005 . denotes .
T1425 5005-5132 sentence denotes First, in the same study [24], recombination in a mouse ES cell line that expresses only truncated BRCA2 protein was measured.
T1426 5006-5011 RB denotes First
T1427 5123-5131 VBN denotes measured
T1428 5011-5013 , denotes ,
T1429 5013-5015 IN denotes in
T1430 5016-5019 DT denotes the
T1431 5025-5030 NN denotes study
T1432 5020-5024 JJ denotes same
T1433 5031-5032 -LRB- denotes [
T1434 5032-5034 CD denotes 24
T1435 5034-5035 -RRB- denotes ]
T1436 5035-5037 , denotes ,
T1437 5037-5050 NN denotes recombination
T1438 5051-5053 IN denotes in
T1439 5054-5055 DT denotes a
T1440 5070-5074 NN denotes line
T1441 5056-5061 NN denotes mouse
T1442 5062-5064 NN denotes ES
T1443 5065-5069 NN denotes cell
T1444 5075-5079 WDT denotes that
T1445 5080-5089 VBZ denotes expresses
T1446 5090-5094 RB denotes only
T1447 5111-5118 NN denotes protein
T1448 5095-5104 VBN denotes truncated
T1449 5105-5110 NN denotes BRCA2
T1450 5119-5122 VBD denotes was
T1451 5131-5132 . denotes .
T1452 5132-5293 sentence denotes This line was found to have lower recombination efficiency than isogenic cells expressing full-length BRCA2 (the defect observed was about fivefold to sixfold).
T1453 5133-5137 DT denotes This
T1454 5138-5142 NN denotes line
T1455 5147-5152 VBN denotes found
T1456 5143-5146 VBD denotes was
T1457 5153-5155 TO denotes to
T1458 5156-5160 VB denotes have
T1459 5161-5166 JJR denotes lower
T1460 5181-5191 NN denotes efficiency
T1461 5167-5180 NN denotes recombination
T1462 5192-5196 IN denotes than
T1463 5197-5205 JJ denotes isogenic
T1464 5206-5211 NNS denotes cells
T1465 5212-5222 VBG denotes expressing
T1466 5223-5227 JJ denotes full
T1467 5228-5234 NN denotes length
T1468 5227-5228 HYPH denotes -
T1469 5235-5240 NN denotes BRCA2
T1470 5241-5242 -LRB- denotes (
T1471 5262-5265 VBD denotes was
T1472 5242-5245 DT denotes the
T1473 5246-5252 NN denotes defect
T1474 5253-5261 VBN denotes observed
T1475 5266-5271 RB denotes about
T1476 5284-5291 RB denotes sixfold
T1477 5272-5280 RB denotes fivefold
T1478 5281-5283 IN denotes to
T1479 5291-5292 -RRB- denotes )
T1480 5292-5293 . denotes .
T1481 5293-5492 sentence denotes Second, in an independent study, Powell was able to compare derivatives of CAPAN-1 that differed only in their ability to express full-length BRCA2 protein (S. Powell, personal communication, 2001).
T1482 5294-5300 RB denotes Second
T1483 5334-5337 VBD denotes was
T1484 5300-5302 , denotes ,
T1485 5302-5304 IN denotes in
T1486 5305-5307 DT denotes an
T1487 5320-5325 NN denotes study
T1488 5308-5319 JJ denotes independent
T1489 5325-5327 , denotes ,
T1490 5327-5333 NNP denotes Powell
T1491 5338-5342 JJ denotes able
T1492 5343-5345 TO denotes to
T1493 5346-5353 VB denotes compare
T1494 5354-5365 NNS denotes derivatives
T1495 5366-5368 IN denotes of
T1496 5369-5374 NN denotes CAPAN
T1497 5374-5375 HYPH denotes -
T1498 5375-5376 CD denotes 1
T1499 5377-5381 WDT denotes that
T1500 5382-5390 VBD denotes differed
T1501 5391-5395 RB denotes only
T1502 5396-5398 IN denotes in
T1503 5399-5404 PRP$ denotes their
T1504 5405-5412 NN denotes ability
T1505 5413-5415 TO denotes to
T1506 5416-5423 VB denotes express
T1507 5424-5428 JJ denotes full
T1508 5429-5435 NN denotes length
T1509 5428-5429 HYPH denotes -
T1510 5442-5449 NN denotes protein
T1511 5436-5441 NN denotes BRCA2
T1512 5450-5451 -LRB- denotes (
T1513 5454-5460 NNP denotes Powell
T1514 5451-5453 NNP denotes S.
T1515 5460-5462 , denotes ,
T1516 5462-5470 JJ denotes personal
T1517 5471-5484 NN denotes communication
T1518 5484-5486 , denotes ,
T1519 5486-5490 CD denotes 2001
T1520 5490-5491 -RRB- denotes )
T1521 5491-5492 . denotes .
T1522 5492-5681 sentence denotes In these experiments, the derivative expressing full-length BRCA2 yielded 10-fold more recombinants than controls expressing only truncated BRCA2 (S. Powell, personal communication, 2001).
T1523 5493-5495 IN denotes In
T1524 5559-5566 VBD denotes yielded
T1525 5496-5501 DT denotes these
T1526 5502-5513 NNS denotes experiments
T1527 5513-5515 , denotes ,
T1528 5515-5518 DT denotes the
T1529 5519-5529 NN denotes derivative
T1530 5530-5540 VBG denotes expressing
T1531 5541-5545 JJ denotes full
T1532 5546-5552 NN denotes length
T1533 5545-5546 HYPH denotes -
T1534 5553-5558 NN denotes BRCA2
T1535 5567-5574 RB denotes 10-fold
T1536 5575-5579 JJR denotes more
T1537 5580-5592 NNS denotes recombinants
T1538 5593-5597 IN denotes than
T1539 5598-5606 NNS denotes controls
T1540 5607-5617 VBG denotes expressing
T1541 5618-5622 RB denotes only
T1542 5633-5638 NN denotes BRCA2
T1543 5623-5632 VBN denotes truncated
T1544 5639-5640 -LRB- denotes (
T1545 5643-5649 NNP denotes Powell
T1546 5640-5642 NNP denotes S.
T1547 5649-5651 , denotes ,
T1548 5651-5659 JJ denotes personal
T1549 5660-5673 NN denotes communication
T1550 5673-5675 , denotes ,
T1551 5675-5679 CD denotes 2001
T1552 5679-5680 -RRB- denotes )
T1553 5680-5681 . denotes .
T1554 5681-5977 sentence denotes While the experimental design of these experiments was somewhat different from that of Moyna-han et al [24], the results raise the possibility that CAPAN-1 cells have more than one mutation that lowers the efficiency of recombinational repair relative to that observed in other human cell lines.
T1555 5682-5687 IN denotes While
T1556 5733-5736 VBD denotes was
T1557 5688-5691 DT denotes the
T1558 5705-5711 NN denotes design
T1559 5692-5704 JJ denotes experimental
T1560 5712-5714 IN denotes of
T1561 5715-5720 DT denotes these
T1562 5721-5732 NNS denotes experiments
T1563 5803-5808 VBP denotes raise
T1564 5737-5745 RB denotes somewhat
T1565 5746-5755 JJ denotes different
T1566 5756-5760 IN denotes from
T1567 5761-5765 DT denotes that
T1568 5766-5768 IN denotes of
T1569 5769-5774 NNP denotes Moyna
T1570 5775-5778 NNP denotes han
T1571 5774-5775 HYPH denotes -
T1572 5779-5781 FW denotes et
T1573 5782-5784 FW denotes al
T1574 5785-5786 -LRB- denotes [
T1575 5786-5788 CD denotes 24
T1576 5788-5789 -RRB- denotes ]
T1577 5789-5791 , denotes ,
T1578 5791-5794 DT denotes the
T1579 5795-5802 NNS denotes results
T1580 5809-5812 DT denotes the
T1581 5813-5824 NN denotes possibility
T1582 5825-5829 IN denotes that
T1583 5844-5848 VBP denotes have
T1584 5830-5835 NN denotes CAPAN
T1585 5838-5843 NNS denotes cells
T1586 5835-5836 HYPH denotes -
T1587 5836-5837 CD denotes 1
T1588 5849-5853 JJR denotes more
T1589 5859-5862 CD denotes one
T1590 5854-5858 IN denotes than
T1591 5863-5871 NN denotes mutation
T1592 5872-5876 WDT denotes that
T1593 5877-5883 VBZ denotes lowers
T1594 5884-5887 DT denotes the
T1595 5888-5898 NN denotes efficiency
T1596 5899-5901 IN denotes of
T1597 5902-5917 JJ denotes recombinational
T1598 5918-5924 NN denotes repair
T1599 5925-5933 JJ denotes relative
T1600 5934-5936 IN denotes to
T1601 5937-5941 DT denotes that
T1602 5942-5950 VBN denotes observed
T1603 5951-5953 IN denotes in
T1604 5954-5959 JJ denotes other
T1605 5971-5976 NNS denotes lines
T1606 5960-5965 JJ denotes human
T1607 5966-5970 NN denotes cell
T1608 5976-5977 . denotes .
T1609 5977-6158 sentence denotes Conversely, the BRCA2 defect in CAPAN-1 could be fully responsible for the 100-fold defect in recombination if the level of BRCA2 complementation observed by Powell was incomplete.
T1610 5978-5988 RB denotes Conversely
T1611 6024-6026 VB denotes be
T1612 5988-5990 , denotes ,
T1613 5990-5993 DT denotes the
T1614 6000-6006 NN denotes defect
T1615 5994-5999 NN denotes BRCA2
T1616 6007-6009 IN denotes in
T1617 6010-6015 NN denotes CAPAN
T1618 6015-6016 HYPH denotes -
T1619 6016-6017 CD denotes 1
T1620 6018-6023 MD denotes could
T1621 6027-6032 RB denotes fully
T1622 6033-6044 JJ denotes responsible
T1623 6045-6048 IN denotes for
T1624 6049-6052 DT denotes the
T1625 6062-6068 NN denotes defect
T1626 6053-6061 JJ denotes 100-fold
T1627 6069-6071 IN denotes in
T1628 6072-6085 NN denotes recombination
T1629 6086-6088 IN denotes if
T1630 6143-6146 VBD denotes was
T1631 6089-6092 DT denotes the
T1632 6093-6098 NN denotes level
T1633 6099-6101 IN denotes of
T1634 6102-6107 NN denotes BRCA2
T1635 6108-6123 NN denotes complementation
T1636 6124-6132 VBN denotes observed
T1637 6133-6135 IN denotes by
T1638 6136-6142 NNP denotes Powell
T1639 6147-6157 JJ denotes incomplete
T1640 6157-6158 . denotes .
T1641 6158-6451 sentence denotes Furthermore, the discrepancy between the 100-fold difference between CAPAN-1 and the other human lines as compared with the fivefold to sixfold difference between the Brca2lex1/lex2cells and Brca2+/+ ES cells might simply be accounted for by the fact that Brca2lex1/lex2 is not a null allele.
T1642 6159-6170 RB denotes Furthermore
T1643 6384-6393 VBN denotes accounted
T1644 6170-6172 , denotes ,
T1645 6172-6175 DT denotes the
T1646 6176-6187 NN denotes discrepancy
T1647 6188-6195 IN denotes between
T1648 6196-6199 DT denotes the
T1649 6209-6219 NN denotes difference
T1650 6200-6208 JJ denotes 100-fold
T1651 6220-6227 IN denotes between
T1652 6228-6233 NN denotes CAPAN
T1653 6233-6234 HYPH denotes -
T1654 6234-6235 CD denotes 1
T1655 6236-6239 CC denotes and
T1656 6240-6243 DT denotes the
T1657 6256-6261 NNS denotes lines
T1658 6244-6249 JJ denotes other
T1659 6250-6255 JJ denotes human
T1660 6262-6264 IN denotes as
T1661 6265-6273 VBN denotes compared
T1662 6274-6278 IN denotes with
T1663 6279-6282 DT denotes the
T1664 6303-6313 NN denotes difference
T1665 6283-6291 JJ denotes fivefold
T1666 6295-6302 JJ denotes sixfold
T1667 6292-6294 IN denotes to
T1668 6314-6321 IN denotes between
T1669 6322-6325 DT denotes the
T1670 6340-6345 NNS denotes cells
T1671 6326-6335 NN denotes Brca2lex1
T1672 6336-6340 NN denotes lex2
T1673 6335-6336 HYPH denotes /
T1674 6346-6349 CC denotes and
T1675 6350-6355 NN denotes Brca2
T1676 6362-6367 NNS denotes cells
T1677 6355-6356 SYM denotes +
T1678 6356-6357 HYPH denotes /
T1679 6357-6358 SYM denotes +
T1680 6359-6361 NN denotes ES
T1681 6368-6373 MD denotes might
T1682 6374-6380 RB denotes simply
T1683 6381-6383 VB denotes be
T1684 6394-6397 IN denotes for
T1685 6398-6400 IN denotes by
T1686 6401-6404 DT denotes the
T1687 6405-6409 NN denotes fact
T1688 6410-6414 IN denotes that
T1689 6430-6432 VBZ denotes is
T1690 6415-6424 NN denotes Brca2lex1
T1691 6425-6429 NN denotes lex2
T1692 6424-6425 HYPH denotes /
T1693 6433-6436 RB denotes not
T1694 6437-6438 DT denotes a
T1695 6444-6450 NN denotes allele
T1696 6439-6443 JJ denotes null
T1697 6450-6451 . denotes .
T1698 6451-6558 sentence denotes Additional studies are likely to shed light on the efficiency of BRCA2-independent recombination pathways.
T1699 6452-6462 JJ denotes Additional
T1700 6463-6470 NNS denotes studies
T1701 6471-6474 VBP denotes are
T1702 6475-6481 JJ denotes likely
T1703 6482-6484 TO denotes to
T1704 6485-6489 VB denotes shed
T1705 6490-6495 NN denotes light
T1706 6496-6498 IN denotes on
T1707 6499-6502 DT denotes the
T1708 6503-6513 NN denotes efficiency
T1709 6514-6516 IN denotes of
T1710 6517-6522 NN denotes BRCA2
T1711 6523-6534 JJ denotes independent
T1712 6522-6523 HYPH denotes -
T1713 6549-6557 NNS denotes pathways
T1714 6535-6548 NN denotes recombination
T1715 6557-6558 . denotes .
T1716 6558-6692 sentence denotes Taken together, the results indicate that BRCA2 is indeed required for high levels of recombinational repair in both human and mouse.
T1717 6559-6564 VBN denotes Taken
T1718 6587-6595 VBP denotes indicate
T1719 6565-6573 RB denotes together
T1720 6573-6575 , denotes ,
T1721 6575-6578 DT denotes the
T1722 6579-6586 NNS denotes results
T1723 6596-6600 IN denotes that
T1724 6617-6625 VBN denotes required
T1725 6601-6606 NN denotes BRCA2
T1726 6607-6609 VBZ denotes is
T1727 6610-6616 RB denotes indeed
T1728 6626-6629 IN denotes for
T1729 6630-6634 JJ denotes high
T1730 6635-6641 NNS denotes levels
T1731 6642-6644 IN denotes of
T1732 6645-6660 JJ denotes recombinational
T1733 6661-6667 NN denotes repair
T1734 6668-6670 IN denotes in
T1735 6671-6675 CC denotes both
T1736 6686-6691 NN denotes mouse
T1737 6676-6681 JJ denotes human
T1738 6682-6685 CC denotes and
T1739 6691-6692 . denotes .
T2154 6734-6737 DT denotes The
T2155 6745-6750 NN denotes paper
T2156 6738-6744 JJ denotes second
T2157 6793-6795 VBZ denotes is
T2158 6751-6760 VBG denotes providing
T2159 6761-6769 NN denotes evidence
T2160 6769-6771 , denotes ,
T2161 6771-6773 IN denotes by
T2162 6774-6780 NNP denotes Davies
T2163 6781-6783 FW denotes et
T2164 6784-6786 FW denotes al
T2165 6787-6788 -LRB- denotes [
T2166 6788-6790 CD denotes 26
T2167 6790-6791 -RRB- denotes ]
T2168 6791-6793 , denotes ,
T2169 6796-6797 DT denotes a
T2170 6810-6815 NN denotes study
T2171 6798-6809 JJ denotes biochemical
T2172 6816-6818 IN denotes of
T2173 6819-6822 DT denotes the
T2174 6823-6834 NN denotes interaction
T2175 6835-6842 IN denotes between
T2176 6843-6846 DT denotes the
T2177 6870-6875 NN denotes RAD51
T2178 6847-6857 JJ denotes homologous
T2179 6858-6869 NN denotes recombinase
T2180 6876-6879 CC denotes and
T2181 6880-6881 DT denotes a
T2182 6882-6889 NN denotes peptide
T2183 6890-6900 VBG denotes consisting
T2184 6901-6903 IN denotes of
T2185 6904-6907 CD denotes one
T2186 6908-6910 IN denotes of
T2187 6911-6914 DT denotes the
T2188 6925-6932 NNS denotes repeats
T2189 6915-6920 CD denotes eight
T2190 6921-6924 NN denotes BRC
T2191 6933-6937 IN denotes from
T2192 6938-6943 JJ denotes human
T2193 6944-6949 NN denotes BRCA2
T2194 6949-6950 . denotes .
T2195 6950-7077 sentence denotes Expression of a single BRC repeat (BRC4) had previously been reported to act as an inhibitor of DNA repair by Chen et al [27].
T2196 6951-6961 NN denotes Expression
T2197 7012-7020 VBN denotes reported
T2198 6962-6964 IN denotes of
T2199 6965-6966 DT denotes a
T2200 6978-6984 NN denotes repeat
T2201 6967-6973 JJ denotes single
T2202 6974-6977 NN denotes BRC
T2203 6985-6986 -LRB- denotes (
T2204 6986-6990 NN denotes BRC4
T2205 6990-6991 -RRB- denotes )
T2206 6992-6995 VBD denotes had
T2207 6996-7006 RB denotes previously
T2208 7007-7011 VBN denotes been
T2209 7021-7023 TO denotes to
T2210 7024-7027 VB denotes act
T2211 7028-7030 IN denotes as
T2212 7031-7033 DT denotes an
T2213 7034-7043 NN denotes inhibitor
T2214 7044-7046 IN denotes of
T2215 7047-7050 NN denotes DNA
T2216 7051-7057 NN denotes repair
T2217 7058-7060 IN denotes by
T2218 7061-7065 NNP denotes Chen
T2219 7066-7068 FW denotes et
T2220 7069-7071 FW denotes al
T2221 7072-7073 -LRB- denotes [
T2222 7073-7075 CD denotes 27
T2223 7075-7076 -RRB- denotes ]
T2224 7076-7077 . denotes .
T2225 7077-7345 sentence denotes These investigators showed that expression of constructs containing the BRC4 repeat in MCF-7 cells enhances the radiosensitivity of cells and blocks both the G2/M delay associated with damage and the ability of the transfected cells to assembly subnuclear RAD51 foci.
T2226 7078-7083 DT denotes These
T2227 7084-7097 NNS denotes investigators
T2228 7098-7104 VBD denotes showed
T2229 7105-7109 IN denotes that
T2230 7177-7185 VBZ denotes enhances
T2231 7110-7120 NN denotes expression
T2232 7121-7123 IN denotes of
T2233 7124-7134 NNS denotes constructs
T2234 7135-7145 VBG denotes containing
T2235 7146-7149 DT denotes the
T2236 7155-7161 NN denotes repeat
T2237 7150-7154 NN denotes BRC4
T2238 7162-7164 IN denotes in
T2239 7165-7168 NN denotes MCF
T2240 7171-7176 NNS denotes cells
T2241 7168-7169 HYPH denotes -
T2242 7169-7170 CD denotes 7
T2243 7186-7189 DT denotes the
T2244 7190-7206 NN denotes radiosensitivity
T2245 7207-7209 IN denotes of
T2246 7210-7215 NNS denotes cells
T2247 7216-7219 CC denotes and
T2248 7220-7226 VBZ denotes blocks
T2249 7227-7231 CC denotes both
T2250 7241-7246 NN denotes delay
T2251 7232-7235 DT denotes the
T2252 7236-7238 NN denotes G2
T2253 7239-7240 NN denotes M
T2254 7238-7239 HYPH denotes /
T2255 7247-7257 VBN denotes associated
T2256 7258-7262 IN denotes with
T2257 7263-7269 NN denotes damage
T2258 7270-7273 CC denotes and
T2259 7274-7277 DT denotes the
T2260 7278-7285 NN denotes ability
T2261 7286-7288 IN denotes of
T2262 7289-7292 DT denotes the
T2263 7305-7310 NNS denotes cells
T2264 7293-7304 VBN denotes transfected
T2265 7311-7313 TO denotes to
T2266 7314-7322 VB denotes assembly
T2267 7323-7333 JJ denotes subnuclear
T2268 7340-7344 NNS denotes foci
T2269 7334-7339 NN denotes RAD51
T2270 7344-7345 . denotes .
T2271 7345-7506 sentence denotes In the paper by Davies et al, data provide evidence for a plausible mechanism of the dominant negative effect associated with expression of a single BRC repeat.
T2272 7346-7348 IN denotes In
T2273 7381-7388 VBP denotes provide
T2274 7349-7352 DT denotes the
T2275 7353-7358 NN denotes paper
T2276 7359-7361 IN denotes by
T2277 7362-7368 NNP denotes Davies
T2278 7369-7371 FW denotes et
T2279 7372-7374 FW denotes al
T2280 7374-7376 , denotes ,
T2281 7376-7380 NNS denotes data
T2282 7389-7397 NN denotes evidence
T2283 7398-7401 IN denotes for
T2284 7402-7403 DT denotes a
T2285 7414-7423 NN denotes mechanism
T2286 7404-7413 JJ denotes plausible
T2287 7424-7426 IN denotes of
T2288 7427-7430 DT denotes the
T2289 7449-7455 NN denotes effect
T2290 7431-7439 JJ denotes dominant
T2291 7440-7448 JJ denotes negative
T2292 7456-7466 VBN denotes associated
T2293 7467-7471 IN denotes with
T2294 7472-7482 NN denotes expression
T2295 7483-7485 IN denotes of
T2296 7486-7487 DT denotes a
T2297 7499-7505 NN denotes repeat
T2298 7488-7494 JJ denotes single
T2299 7495-7498 NN denotes BRC
T2300 7505-7506 . denotes .
T2301 7506-7678 sentence denotes DNA binding assays and electron microscopy methods were used to show that a BRC3 peptide interferes with the ability of RAD51 to assemble into oligomeric filaments on DNA.
T2302 7507-7510 NN denotes DNA
T2303 7519-7525 NNS denotes assays
T2304 7511-7518 NN denotes binding
T2305 7563-7567 VBN denotes used
T2306 7526-7529 CC denotes and
T2307 7530-7538 NN denotes electron
T2308 7539-7549 NN denotes microscopy
T2309 7550-7557 NNS denotes methods
T2310 7558-7562 VBD denotes were
T2311 7568-7570 TO denotes to
T2312 7571-7575 VB denotes show
T2313 7576-7580 IN denotes that
T2314 7596-7606 VBZ denotes interferes
T2315 7581-7582 DT denotes a
T2316 7588-7595 NN denotes peptide
T2317 7583-7587 NN denotes BRC3
T2318 7607-7611 IN denotes with
T2319 7612-7615 DT denotes the
T2320 7616-7623 NN denotes ability
T2321 7624-7626 IN denotes of
T2322 7627-7632 NN denotes RAD51
T2323 7633-7635 TO denotes to
T2324 7636-7644 VB denotes assemble
T2325 7645-7649 IN denotes into
T2326 7650-7660 JJ denotes oligomeric
T2327 7661-7670 NNS denotes filaments
T2328 7671-7673 IN denotes on
T2329 7674-7677 NN denotes DNA
T2330 7677-7678 . denotes .
T2331 7678-7749 sentence denotes A BRC4 peptide was also reported to inhibit the RAD51–DNA interaction.
T2332 7679-7680 DT denotes A
T2333 7686-7693 NN denotes peptide
T2334 7681-7685 NN denotes BRC4
T2335 7703-7711 VBN denotes reported
T2336 7694-7697 VBD denotes was
T2337 7698-7702 RB denotes also
T2338 7712-7714 TO denotes to
T2339 7715-7722 VB denotes inhibit
T2340 7723-7726 DT denotes the
T2341 7737-7748 NN denotes interaction
T2342 7727-7732 NN denotes RAD51
T2343 7733-7736 NN denotes DNA
T2344 7732-7733 HYPH denotes –
T2345 7748-7749 . denotes .
T2346 7749-7898 sentence denotes These experiments raise the possibility that the full-length BRCA2 protein could act as a negative regulator of inappropriate RAD51–DNA interaction.
T2347 7750-7755 DT denotes These
T2348 7756-7767 NNS denotes experiments
T2349 7768-7773 VBP denotes raise
T2350 7774-7777 DT denotes the
T2351 7778-7789 NN denotes possibility
T2352 7790-7794 IN denotes that
T2353 7831-7834 VB denotes act
T2354 7795-7798 DT denotes the
T2355 7817-7824 NN denotes protein
T2356 7799-7803 JJ denotes full
T2357 7804-7810 NN denotes length
T2358 7803-7804 HYPH denotes -
T2359 7811-7816 NN denotes BRCA2
T2360 7825-7830 MD denotes could
T2361 7835-7837 IN denotes as
T2362 7838-7839 DT denotes a
T2363 7849-7858 NN denotes regulator
T2364 7840-7848 JJ denotes negative
T2365 7859-7861 IN denotes of
T2366 7862-7875 JJ denotes inappropriate
T2367 7886-7897 NN denotes interaction
T2368 7876-7881 NN denotes RAD51
T2369 7882-7885 NN denotes DNA
T2370 7881-7882 HYPH denotes –
T2371 7897-7898 . denotes .
T2372 7898-8082 sentence denotes The final experiment reported by Davies et al [26] is particularly important with respect to understanding the mechanism(s) through which BRCA2 promotes RAD51-dependent recombination.
T2373 7899-7902 DT denotes The
T2374 7909-7919 NN denotes experiment
T2375 7903-7908 JJ denotes final
T2376 7950-7952 VBZ denotes is
T2377 7920-7928 VBN denotes reported
T2378 7929-7931 IN denotes by
T2379 7932-7938 NNP denotes Davies
T2380 7939-7941 FW denotes et
T2381 7942-7944 FW denotes al
T2382 7945-7946 -LRB- denotes [
T2383 7946-7948 CD denotes 26
T2384 7948-7949 -RRB- denotes ]
T2385 7953-7965 RB denotes particularly
T2386 7966-7975 JJ denotes important
T2387 7976-7980 IN denotes with
T2388 7981-7988 NN denotes respect
T2389 7989-7991 IN denotes to
T2390 7992-8005 VBG denotes understanding
T2391 8006-8009 DT denotes the
T2392 8020-8021 NNS denotes s
T2393 8010-8019 NN denotes mechanism
T2394 8019-8020 -LRB- denotes (
T2395 8021-8022 -RRB- denotes )
T2396 8023-8030 IN denotes through
T2397 8043-8051 VBZ denotes promotes
T2398 8031-8036 WDT denotes which
T2399 8037-8042 NN denotes BRCA2
T2400 8052-8057 NN denotes RAD51
T2401 8058-8067 JJ denotes dependent
T2402 8057-8058 HYPH denotes -
T2403 8068-8081 NN denotes recombination
T2404 8081-8082 . denotes .
T2405 8082-8198 sentence denotes The localization of RAD51 was examined in CAPAN-1 cells, the same human tumor line examined by Moynahan et al [24].
T2406 8083-8086 DT denotes The
T2407 8087-8099 NN denotes localization
T2408 8113-8121 VBN denotes examined
T2409 8100-8102 IN denotes of
T2410 8103-8108 NN denotes RAD51
T2411 8109-8112 VBD denotes was
T2412 8122-8124 IN denotes in
T2413 8125-8130 NN denotes CAPAN
T2414 8133-8138 NNS denotes cells
T2415 8130-8131 HYPH denotes -
T2416 8131-8132 CD denotes 1
T2417 8138-8140 , denotes ,
T2418 8140-8143 DT denotes the
T2419 8161-8165 NN denotes line
T2420 8144-8148 JJ denotes same
T2421 8149-8154 JJ denotes human
T2422 8155-8160 NN denotes tumor
T2423 8166-8174 VBN denotes examined
T2424 8175-8177 IN denotes by
T2425 8178-8186 NNP denotes Moynahan
T2426 8187-8189 FW denotes et
T2427 8190-8192 FW denotes al
T2428 8193-8194 -LRB- denotes [
T2429 8194-8196 CD denotes 24
T2430 8196-8197 -RRB- denotes ]
T2431 8197-8198 . denotes .
T2432 8198-8433 sentence denotes It had been shown previously that the small amount of truncated BRCA2 protein expressed by CAPAN-1 cells was mislocalized to the cytoplasm, consistent with the fact that the truncation protein lacks a nuclear localization signal [28].
T2433 8199-8201 PRP denotes It
T2434 8211-8216 VBN denotes shown
T2435 8202-8205 VBD denotes had
T2436 8206-8210 VBN denotes been
T2437 8217-8227 RB denotes previously
T2438 8228-8232 IN denotes that
T2439 8308-8320 VBN denotes mislocalized
T2440 8233-8236 DT denotes the
T2441 8243-8249 NN denotes amount
T2442 8237-8242 JJ denotes small
T2443 8250-8252 IN denotes of
T2444 8253-8262 VBN denotes truncated
T2445 8269-8276 NN denotes protein
T2446 8263-8268 NN denotes BRCA2
T2447 8277-8286 VBN denotes expressed
T2448 8287-8289 IN denotes by
T2449 8290-8295 NN denotes CAPAN
T2450 8298-8303 NNS denotes cells
T2451 8295-8296 HYPH denotes -
T2452 8296-8297 CD denotes 1
T2453 8304-8307 VBD denotes was
T2454 8321-8323 IN denotes to
T2455 8324-8327 DT denotes the
T2456 8328-8337 NN denotes cytoplasm
T2457 8337-8339 , denotes ,
T2458 8339-8349 JJ denotes consistent
T2459 8350-8354 IN denotes with
T2460 8355-8358 DT denotes the
T2461 8359-8363 NN denotes fact
T2462 8364-8368 IN denotes that
T2463 8392-8397 VBZ denotes lacks
T2464 8369-8372 DT denotes the
T2465 8384-8391 NN denotes protein
T2466 8373-8383 NN denotes truncation
T2467 8398-8399 DT denotes a
T2468 8421-8427 NN denotes signal
T2469 8400-8407 JJ denotes nuclear
T2470 8408-8420 NN denotes localization
T2471 8428-8429 -LRB- denotes [
T2472 8429-8431 CD denotes 28
T2473 8431-8432 -RRB- denotes ]
T2474 8432-8433 . denotes .
T2475 8433-8605 sentence denotes Davies et al showed CAPAN-1 cells to be defective in nuclear localization of RAD51, raising the possibility that RAD51 is normally carried to the nucleus by binding BRCA2.
T2476 8434-8440 NNP denotes Davies
T2477 8447-8453 VBD denotes showed
T2478 8441-8443 FW denotes et
T2479 8444-8446 FW denotes al
T2480 8454-8459 NN denotes CAPAN
T2481 8462-8467 NNS denotes cells
T2482 8459-8460 HYPH denotes -
T2483 8460-8461 CD denotes 1
T2484 8471-8473 VB denotes be
T2485 8468-8470 TO denotes to
T2486 8474-8483 JJ denotes defective
T2487 8484-8486 IN denotes in
T2488 8487-8494 JJ denotes nuclear
T2489 8495-8507 NN denotes localization
T2490 8508-8510 IN denotes of
T2491 8511-8516 NN denotes RAD51
T2492 8516-8518 , denotes ,
T2493 8518-8525 VBG denotes raising
T2494 8526-8529 DT denotes the
T2495 8530-8541 NN denotes possibility
T2496 8542-8546 IN denotes that
T2497 8565-8572 VBN denotes carried
T2498 8547-8552 NN denotes RAD51
T2499 8553-8555 VBZ denotes is
T2500 8556-8564 RB denotes normally
T2501 8573-8575 IN denotes to
T2502 8576-8579 DT denotes the
T2503 8580-8587 NN denotes nucleus
T2504 8588-8590 IN denotes by
T2505 8591-8598 VBG denotes binding
T2506 8599-8604 NN denotes BRCA2
T2507 8604-8605 . denotes .
T2508 8605-8798 sentence denotes It is possible that RAD51 may alternatively be capable of BRCA2-independent transport, but mislocalization of BRCA2 to the cytoplasm may sequester RAD51 and block its normal mode of transport.
T2509 8606-8608 PRP denotes It
T2510 8609-8611 VBZ denotes is
T2511 8612-8620 JJ denotes possible
T2512 8621-8625 IN denotes that
T2513 8650-8652 VB denotes be
T2514 8626-8631 NN denotes RAD51
T2515 8632-8635 MD denotes may
T2516 8636-8649 RB denotes alternatively
T2517 8653-8660 JJ denotes capable
T2518 8661-8663 IN denotes of
T2519 8664-8669 NN denotes BRCA2
T2520 8670-8681 JJ denotes independent
T2521 8669-8670 HYPH denotes -
T2522 8682-8691 NN denotes transport
T2523 8691-8693 , denotes ,
T2524 8693-8696 CC denotes but
T2525 8697-8712 NN denotes mislocalization
T2526 8743-8752 VB denotes sequester
T2527 8713-8715 IN denotes of
T2528 8716-8721 NN denotes BRCA2
T2529 8722-8724 IN denotes to
T2530 8725-8728 DT denotes the
T2531 8729-8738 NN denotes cytoplasm
T2532 8739-8742 MD denotes may
T2533 8753-8758 NN denotes RAD51
T2534 8759-8762 CC denotes and
T2535 8763-8768 VB denotes block
T2536 8769-8772 PRP$ denotes its
T2537 8780-8784 NN denotes mode
T2538 8773-8779 JJ denotes normal
T2539 8785-8787 IN denotes of
T2540 8788-8797 NN denotes transport
T2541 8797-8798 . denotes .
T2542 8798-8973 sentence denotes Support for the hypothesis that the observed mislocalization of RAD51 is an indirect consequence of the BRCA2 defect requires reintroduction of functional BRCA2 into CAPAN-1.
T2543 8799-8806 NN denotes Support
T2544 8916-8924 VBZ denotes requires
T2545 8807-8810 IN denotes for
T2546 8811-8814 DT denotes the
T2547 8815-8825 NN denotes hypothesis
T2548 8826-8830 IN denotes that
T2549 8869-8871 VBZ denotes is
T2550 8831-8834 DT denotes the
T2551 8844-8859 NN denotes mislocalization
T2552 8835-8843 VBN denotes observed
T2553 8860-8862 IN denotes of
T2554 8863-8868 NN denotes RAD51
T2555 8872-8874 DT denotes an
T2556 8884-8895 NN denotes consequence
T2557 8875-8883 JJ denotes indirect
T2558 8896-8898 IN denotes of
T2559 8899-8902 DT denotes the
T2560 8909-8915 NN denotes defect
T2561 8903-8908 NN denotes BRCA2
T2562 8925-8939 NN denotes reintroduction
T2563 8940-8942 IN denotes of
T2564 8943-8953 JJ denotes functional
T2565 8954-8959 NN denotes BRCA2
T2566 8960-8964 IN denotes into
T2567 8965-8970 NN denotes CAPAN
T2568 8970-8971 HYPH denotes -
T2569 8971-8972 CD denotes 1
T2570 8972-8973 . denotes .
T2571 8973-9170 sentence denotes Given the elaborate nature of the RAD51–BRCA2 interaction, it seems somewhat unlikely that the only role of BRCA2 in promoting recombination is to serve as a specific nuclear transporter of RAD51.
T2572 8974-8979 VBN denotes Given
T2573 9036-9041 VBZ denotes seems
T2574 8980-8983 DT denotes the
T2575 8994-9000 NN denotes nature
T2576 8984-8993 JJ denotes elaborate
T2577 9001-9003 IN denotes of
T2578 9004-9007 DT denotes the
T2579 9020-9031 NN denotes interaction
T2580 9008-9013 NN denotes RAD51
T2581 9014-9019 NN denotes BRCA2
T2582 9013-9014 HYPH denotes –
T2583 9031-9033 , denotes ,
T2584 9033-9035 PRP denotes it
T2585 9042-9050 RB denotes somewhat
T2586 9051-9059 JJ denotes unlikely
T2587 9060-9064 IN denotes that
T2588 9115-9117 VBZ denotes is
T2589 9065-9068 DT denotes the
T2590 9074-9078 NN denotes role
T2591 9069-9073 JJ denotes only
T2592 9079-9081 IN denotes of
T2593 9082-9087 NN denotes BRCA2
T2594 9088-9090 IN denotes in
T2595 9091-9100 VBG denotes promoting
T2596 9101-9114 NN denotes recombination
T2597 9118-9120 TO denotes to
T2598 9121-9126 VB denotes serve
T2599 9127-9129 IN denotes as
T2600 9130-9131 DT denotes a
T2601 9149-9160 NN denotes transporter
T2602 9132-9140 JJ denotes specific
T2603 9141-9148 JJ denotes nuclear
T2604 9161-9163 IN denotes of
T2605 9164-9169 NN denotes RAD51
T2606 9169-9170 . denotes .
T2607 9170-9351 sentence denotes An attractive alternative, favored by Davies et al [26], is that the association of RAD51–BRCA2 is required to maintain RAD51 in a form that is in a state of 'readiness' (Fig. 2a).
T2608 9171-9173 DT denotes An
T2609 9185-9196 NN denotes alternative
T2610 9174-9184 JJ denotes attractive
T2611 9228-9230 VBZ denotes is
T2612 9196-9198 , denotes ,
T2613 9198-9205 VBN denotes favored
T2614 9206-9208 IN denotes by
T2615 9209-9215 NNP denotes Davies
T2616 9216-9218 FW denotes et
T2617 9219-9221 FW denotes al
T2618 9222-9223 -LRB- denotes [
T2619 9223-9225 CD denotes 26
T2620 9225-9226 -RRB- denotes ]
T2621 9226-9228 , denotes ,
T2622 9231-9235 IN denotes that
T2623 9270-9278 VBN denotes required
T2624 9236-9239 DT denotes the
T2625 9240-9251 NN denotes association
T2626 9252-9254 IN denotes of
T2627 9255-9260 NN denotes RAD51
T2628 9261-9266 NN denotes BRCA2
T2629 9260-9261 HYPH denotes –
T2630 9267-9269 VBZ denotes is
T2631 9279-9281 TO denotes to
T2632 9282-9290 VB denotes maintain
T2633 9291-9296 NN denotes RAD51
T2634 9297-9299 IN denotes in
T2635 9300-9301 DT denotes a
T2636 9302-9306 NN denotes form
T2637 9307-9311 WDT denotes that
T2638 9312-9314 VBZ denotes is
T2639 9315-9317 IN denotes in
T2640 9318-9319 DT denotes a
T2641 9320-9325 NN denotes state
T2642 9326-9328 IN denotes of
T2643 9329-9330 `` denotes '
T2644 9330-9339 NN denotes readiness
T2645 9339-9340 '' denotes '
T2646 9341-9342 -LRB- denotes (
T2647 9347-9349 NNP denotes 2a
T2648 9342-9346 NNP denotes Fig.
T2649 9349-9350 -RRB- denotes )
T2650 9350-9351 . denotes .
T2651 9351-9518 sentence denotes In the absence of this interaction with BRCA2, RAD51 might exist in a form that is not capable of being recruited into a functional repair complex when damage occurs.
T2652 9352-9354 IN denotes In
T2653 9411-9416 VB denotes exist
T2654 9355-9358 DT denotes the
T2655 9359-9366 NN denotes absence
T2656 9367-9369 IN denotes of
T2657 9370-9374 DT denotes this
T2658 9375-9386 NN denotes interaction
T2659 9387-9391 IN denotes with
T2660 9392-9397 NN denotes BRCA2
T2661 9397-9399 , denotes ,
T2662 9399-9404 NN denotes RAD51
T2663 9405-9410 MD denotes might
T2664 9417-9419 IN denotes in
T2665 9420-9421 DT denotes a
T2666 9422-9426 NN denotes form
T2667 9427-9431 WDT denotes that
T2668 9432-9434 VBZ denotes is
T2669 9435-9438 RB denotes not
T2670 9439-9446 JJ denotes capable
T2671 9447-9449 IN denotes of
T2672 9450-9455 VBG denotes being
T2673 9456-9465 VBN denotes recruited
T2674 9466-9470 IN denotes into
T2675 9471-9472 DT denotes a
T2676 9491-9498 NN denotes complex
T2677 9473-9483 JJ denotes functional
T2678 9484-9490 NN denotes repair
T2679 9499-9503 WRB denotes when
T2680 9511-9517 VBZ denotes occurs
T2681 9504-9510 NN denotes damage
T2682 9517-9518 . denotes .
T2683 9518-9670 sentence denotes For example, previous studies indicated that RAD51 shows little binding preference for single-strand DNA (ssDNA) over double-strand DNA (dsDNA) [3,29].
T2684 9519-9522 IN denotes For
T2685 9549-9558 VBD denotes indicated
T2686 9523-9530 NN denotes example
T2687 9530-9532 , denotes ,
T2688 9532-9540 JJ denotes previous
T2689 9541-9548 NNS denotes studies
T2690 9559-9563 IN denotes that
T2691 9570-9575 VBZ denotes shows
T2692 9564-9569 NN denotes RAD51
T2693 9576-9582 JJ denotes little
T2694 9591-9601 NN denotes preference
T2695 9583-9590 NN denotes binding
T2696 9602-9605 IN denotes for
T2697 9606-9612 JJ denotes single
T2698 9613-9619 NN denotes strand
T2699 9612-9613 HYPH denotes -
T2700 9620-9623 NN denotes DNA
T2701 9624-9625 -LRB- denotes (
T2702 9625-9630 NN denotes ssDNA
T2703 9630-9631 -RRB- denotes )
T2704 9632-9636 IN denotes over
T2705 9637-9643 JJ denotes double
T2706 9644-9650 NN denotes strand
T2707 9643-9644 HYPH denotes -
T2708 9651-9654 NN denotes DNA
T2709 9655-9656 -LRB- denotes (
T2710 9656-9661 NN denotes dsDNA
T2711 9661-9662 -RRB- denotes )
T2712 9663-9664 -LRB- denotes [
T2713 9666-9668 CD denotes 29
T2714 9664-9665 CD denotes 3
T2715 9665-9666 , denotes ,
T2716 9668-9669 -RRB- denotes ]
T2717 9669-9670 . denotes .
T2718 9670-9842 sentence denotes This result could mean that regulatory factors, such as BRCA2, are required to suppress RAD51–dsDNA interactions to prevent sequestration of RAD51 in a nonfunctional form.
T2719 9671-9675 DT denotes This
T2720 9676-9682 NN denotes result
T2721 9689-9693 VB denotes mean
T2722 9683-9688 MD denotes could
T2723 9694-9698 IN denotes that
T2724 9738-9746 VBN denotes required
T2725 9699-9709 JJ denotes regulatory
T2726 9710-9717 NNS denotes factors
T2727 9717-9719 , denotes ,
T2728 9719-9723 JJ denotes such
T2729 9724-9726 IN denotes as
T2730 9727-9732 NN denotes BRCA2
T2731 9732-9734 , denotes ,
T2732 9734-9737 VBP denotes are
T2733 9747-9749 TO denotes to
T2734 9750-9758 VB denotes suppress
T2735 9759-9764 NN denotes RAD51
T2736 9765-9770 NN denotes dsDNA
T2737 9764-9765 HYPH denotes –
T2738 9771-9783 NNS denotes interactions
T2739 9784-9786 TO denotes to
T2740 9787-9794 VB denotes prevent
T2741 9795-9808 NN denotes sequestration
T2742 9809-9811 IN denotes of
T2743 9812-9817 NN denotes RAD51
T2744 9818-9820 IN denotes in
T2745 9821-9822 DT denotes a
T2746 9837-9841 NN denotes form
T2747 9823-9836 JJ denotes nonfunctional
T2748 9841-9842 . denotes .
T2749 9842-10030 sentence denotes Alternatively, or in addition, suppression of RAD51–ssDNA interaction might be important for preventing RAD51 from binding to the regions of ssDNA that form during normal DNA replication.
T2750 9843-9856 RB denotes Alternatively
T2751 9861-9863 IN denotes in
T2752 9856-9858 , denotes ,
T2753 9858-9860 CC denotes or
T2754 9919-9921 VB denotes be
T2755 9864-9872 NN denotes addition
T2756 9872-9874 , denotes ,
T2757 9874-9885 NN denotes suppression
T2758 9886-9888 IN denotes of
T2759 9889-9894 NN denotes RAD51
T2760 9895-9900 NN denotes ssDNA
T2761 9894-9895 HYPH denotes –
T2762 9901-9912 NN denotes interaction
T2763 9913-9918 MD denotes might
T2764 9922-9931 JJ denotes important
T2765 9932-9935 IN denotes for
T2766 9936-9946 VBG denotes preventing
T2767 9947-9952 NN denotes RAD51
T2768 9953-9957 IN denotes from
T2769 9958-9965 VBG denotes binding
T2770 9966-9968 IN denotes to
T2771 9969-9972 DT denotes the
T2772 9973-9980 NNS denotes regions
T2773 9981-9983 IN denotes of
T2774 9984-9989 NN denotes ssDNA
T2775 9990-9994 WDT denotes that
T2776 9995-9999 VBP denotes form
T2777 10000-10006 IN denotes during
T2778 10007-10013 JJ denotes normal
T2779 10018-10029 NN denotes replication
T2780 10014-10017 NN denotes DNA
T2781 10029-10030 . denotes .
T2782 10030-10298 sentence denotes BRCA2 may thus promote RAD51 assembly into recombinational repair complexes via a negative regulatory mechanism (i.e. by blocking RAD51–BRCA2 interaction until damage has occurred and factors required for 'productive' assembly of RAD51 at damaged sites are in place).
T2783 10031-10036 NN denotes BRCA2
T2784 10046-10053 VB denotes promote
T2785 10037-10040 MD denotes may
T2786 10041-10045 RB denotes thus
T2787 10054-10059 NN denotes RAD51
T2788 10060-10068 NN denotes assembly
T2789 10069-10073 IN denotes into
T2790 10074-10089 JJ denotes recombinational
T2791 10097-10106 NNS denotes complexes
T2792 10090-10096 NN denotes repair
T2793 10107-10110 IN denotes via
T2794 10111-10112 DT denotes a
T2795 10133-10142 NN denotes mechanism
T2796 10113-10121 JJ denotes negative
T2797 10122-10132 JJ denotes regulatory
T2798 10143-10144 -LRB- denotes (
T2799 10144-10148 FW denotes i.e.
T2800 10149-10151 IN denotes by
T2801 10152-10160 VBG denotes blocking
T2802 10161-10172 NN denotes RAD51–BRCA2
T2803 10173-10184 NN denotes interaction
T2804 10185-10190 IN denotes until
T2805 10202-10210 VBN denotes occurred
T2806 10191-10197 NN denotes damage
T2807 10198-10201 VBZ denotes has
T2808 10211-10214 CC denotes and
T2809 10215-10222 NNS denotes factors
T2810 10284-10287 VBP denotes are
T2811 10223-10231 VBN denotes required
T2812 10232-10235 IN denotes for
T2813 10236-10237 `` denotes '
T2814 10237-10247 JJ denotes productive
T2815 10249-10257 NN denotes assembly
T2816 10247-10248 '' denotes '
T2817 10258-10260 IN denotes of
T2818 10261-10266 NN denotes RAD51
T2819 10267-10269 IN denotes at
T2820 10270-10277 JJ denotes damaged
T2821 10278-10283 NNS denotes sites
T2822 10288-10290 IN denotes in
T2823 10291-10296 NN denotes place
T2824 10296-10297 -RRB- denotes )
T2825 10297-10298 . denotes .
T2826 10298-10485 sentence denotes As mentioned previously, the ability of a BRC peptide to repress the RAD51–BRCA2 interaction could reflect a role of full-length protein in suppressing unwanted RAD51–BRCA2 interactions.
T2827 10299-10301 IN denotes As
T2828 10302-10311 VBN denotes mentioned
T2829 10398-10405 VB denotes reflect
T2830 10312-10322 RB denotes previously
T2831 10322-10324 , denotes ,
T2832 10324-10327 DT denotes the
T2833 10328-10335 NN denotes ability
T2834 10336-10338 IN denotes of
T2835 10339-10340 DT denotes a
T2836 10345-10352 NN denotes peptide
T2837 10341-10344 NN denotes BRC
T2838 10353-10355 TO denotes to
T2839 10356-10363 VB denotes repress
T2840 10364-10367 DT denotes the
T2841 10380-10391 NN denotes interaction
T2842 10368-10373 NN denotes RAD51
T2843 10373-10374 HYPH denotes –
T2844 10374-10379 NN denotes BRCA2
T2845 10392-10397 MD denotes could
T2846 10406-10407 DT denotes a
T2847 10408-10412 NN denotes role
T2848 10413-10415 IN denotes of
T2849 10416-10420 JJ denotes full
T2850 10421-10427 NN denotes length
T2851 10420-10421 HYPH denotes -
T2852 10428-10435 NN denotes protein
T2853 10436-10438 IN denotes in
T2854 10439-10450 VBG denotes suppressing
T2855 10451-10459 JJ denotes unwanted
T2856 10472-10484 NNS denotes interactions
T2857 10460-10465 NN denotes RAD51
T2858 10466-10471 NN denotes BRCA2
T2859 10465-10466 HYPH denotes –
T2860 10484-10485 . denotes .
T2861 10485-11677 sentence denotes Figure 2 Potential roles of BRCA2 in promoting assembly of Rad51 at sites of DNA damage. Chromosomal DNA is shown as pairs of straight lines, Rad51 as open circles, and BRCA2 as grey bars. (a) Prevention of nonproductive DNA interactions. BRCA2–Rad51 interaction is proposed to suppress RAD51–BRCA2 interactions until DNA damage is present. When damage occurs, Rad51 is recruited to damaged sites where is polymerizes into nucleoprotein filaments. In this model, BRCA2 is not required for assembly of functional complexes at damaged sites, only to prevent a substantial fraction of Rad51 from being sequestered in a nonfunctional form. In a BRCA2-defective cell, mutant Rad51 becomes associated with DNA at random sites and is therefore not readily recruited to sites of damage. (b) Positive regulation. BRCA2 is proposed to be required for Rad51 to assemble into functional recombinational repair complexes at sites of damage. In BRCA-defective cells, Rad51 fails to associate with sites of damage due to lack of an assembly factor. Yet another potential role for BRCA2 in promoting RAD51-dependent recombinational repair is a positive role in assembly of RAD51 at damaged sites (Fig. 2b).
T2862 11521-11524 RB denotes Yet
T2863 11543-11547 NN denotes role
T2864 11525-11532 DT denotes another
T2865 11533-11542 JJ denotes potential
T2866 11610-11612 VBZ denotes is
T2867 11548-11551 IN denotes for
T2868 11552-11557 NN denotes BRCA2
T2869 11558-11560 IN denotes in
T2870 11561-11570 VBG denotes promoting
T2871 11571-11576 NN denotes RAD51
T2872 11577-11586 JJ denotes dependent
T2873 11576-11577 HYPH denotes -
T2874 11603-11609 NN denotes repair
T2875 11587-11602 JJ denotes recombinational
T2876 11613-11614 DT denotes a
T2877 11624-11628 NN denotes role
T2878 11615-11623 JJ denotes positive
T2879 11629-11631 IN denotes in
T2880 11632-11640 NN denotes assembly
T2881 11641-11643 IN denotes of
T2882 11644-11649 NN denotes RAD51
T2883 11650-11652 IN denotes at
T2884 11653-11660 VBN denotes damaged
T2885 11661-11666 NNS denotes sites
T2886 11667-11668 -LRB- denotes (
T2887 11673-11675 NN denotes 2b
T2888 11668-11672 NN denotes Fig.
T2889 11675-11676 -RRB- denotes )
T2890 11676-11677 . denotes .
T2891 11677-11805 sentence denotes Several proteins that interact with RAD51 are thought to 'mediate' assembly of RAD51 at sites of DNA damage (reviewed in [30]).
T2892 11678-11685 JJ denotes Several
T2893 11686-11694 NN denotes proteins
T2894 11724-11731 VBN denotes thought
T2895 11695-11699 WDT denotes that
T2896 11700-11708 VBP denotes interact
T2897 11709-11713 IN denotes with
T2898 11714-11719 NN denotes RAD51
T2899 11720-11723 VBP denotes are
T2900 11732-11734 TO denotes to
T2901 11736-11743 VB denotes mediate
T2902 11735-11736 `` denotes '
T2903 11743-11744 '' denotes '
T2904 11745-11753 NN denotes assembly
T2905 11754-11756 IN denotes of
T2906 11757-11762 NN denotes RAD51
T2907 11763-11765 IN denotes at
T2908 11766-11771 NNS denotes sites
T2909 11772-11774 IN denotes of
T2910 11775-11778 NN denotes DNA
T2911 11779-11785 NN denotes damage
T2912 11786-11787 -LRB- denotes (
T2913 11787-11795 VBN denotes reviewed
T2914 11796-11798 IN denotes in
T2915 11799-11800 -LRB- denotes [
T2916 11800-11802 CD denotes 30
T2917 11802-11803 -RRB- denotes ]
T2918 11803-11804 -RRB- denotes )
T2919 11804-11805 . denotes .
T2920 11805-11956 sentence denotes RAD51 paralogs in particular, including XRCC2, XRCC3, RAD51B, RAD51C and RAD51D, may function as a complex that actively promotes RAD51 assembly [31].
T2921 11806-11811 NN denotes RAD51
T2922 11812-11820 NNS denotes paralogs
T2923 11891-11899 VB denotes function
T2924 11821-11823 IN denotes in
T2925 11824-11834 JJ denotes particular
T2926 11834-11836 , denotes ,
T2927 11836-11845 VBG denotes including
T2928 11846-11851 NN denotes XRCC2
T2929 11851-11853 , denotes ,
T2930 11853-11858 NN denotes XRCC3
T2931 11858-11860 , denotes ,
T2932 11860-11866 NN denotes RAD51B
T2933 11866-11868 , denotes ,
T2934 11868-11874 NN denotes RAD51C
T2935 11875-11878 CC denotes and
T2936 11879-11885 NN denotes RAD51D
T2937 11885-11887 , denotes ,
T2938 11887-11890 MD denotes may
T2939 11900-11902 IN denotes as
T2940 11903-11904 DT denotes a
T2941 11905-11912 NN denotes complex
T2942 11913-11917 WDT denotes that
T2943 11927-11935 VBZ denotes promotes
T2944 11918-11926 RB denotes actively
T2945 11936-11941 NN denotes RAD51
T2946 11942-11950 NN denotes assembly
T2947 11951-11952 -LRB- denotes [
T2948 11952-11954 CD denotes 31
T2949 11954-11955 -RRB- denotes ]
T2950 11955-11956 . denotes .
T2951 11956-12076 sentence denotes BRCA2 could cooperate with RAD51 paralogs in promoting RAD51 assembly, or even provide an alternative assembly pathway.
T2952 11957-11962 NN denotes BRCA2
T2953 11969-11978 VB denotes cooperate
T2954 11963-11968 MD denotes could
T2955 11979-11983 IN denotes with
T2956 11984-11989 NN denotes RAD51
T2957 11990-11998 NNS denotes paralogs
T2958 11999-12001 IN denotes in
T2959 12002-12011 VBG denotes promoting
T2960 12012-12017 NN denotes RAD51
T2961 12018-12026 NN denotes assembly
T2962 12026-12028 , denotes ,
T2963 12028-12030 CC denotes or
T2964 12031-12035 RB denotes even
T2965 12036-12043 VB denotes provide
T2966 12044-12046 DT denotes an
T2967 12068-12075 NN denotes pathway
T2968 12047-12058 JJ denotes alternative
T2969 12059-12067 NN denotes assembly
T2970 12075-12076 . denotes .
T2971 12076-12276 sentence denotes The observation that BRCA2 localizes to damage-induced subnuclear foci with RAD51 seems consistent with the possibility that BRCA2 plays a positive role in assembly of the recombination complex [11].
T2972 12077-12080 DT denotes The
T2973 12081-12092 NN denotes observation
T2974 12159-12164 VBZ denotes seems
T2975 12093-12097 IN denotes that
T2976 12104-12113 VBZ denotes localizes
T2977 12098-12103 NN denotes BRCA2
T2978 12114-12116 IN denotes to
T2979 12117-12123 NN denotes damage
T2980 12124-12131 VBN denotes induced
T2981 12123-12124 HYPH denotes -
T2982 12143-12147 NNS denotes foci
T2983 12132-12142 JJ denotes subnuclear
T2984 12148-12152 IN denotes with
T2985 12153-12158 NN denotes RAD51
T2986 12165-12175 JJ denotes consistent
T2987 12176-12180 IN denotes with
T2988 12181-12184 DT denotes the
T2989 12185-12196 NN denotes possibility
T2990 12197-12201 IN denotes that
T2991 12208-12213 VBZ denotes plays
T2992 12202-12207 NN denotes BRCA2
T2993 12214-12215 DT denotes a
T2994 12225-12229 NN denotes role
T2995 12216-12224 JJ denotes positive
T2996 12230-12232 IN denotes in
T2997 12233-12241 NN denotes assembly
T2998 12242-12244 IN denotes of
T2999 12245-12248 DT denotes the
T3000 12263-12270 NN denotes complex
T3001 12249-12262 NN denotes recombination
T3002 12271-12272 -LRB- denotes [
T3003 12272-12274 CD denotes 11
T3004 12274-12275 -RRB- denotes ]
T3005 12275-12276 . denotes .
T3006 12276-12423 sentence denotes Finally, it is important to note that the two models for the function of the BRCA2–RAD51 interaction shown in Figure 2 are not mutually exclusive.
T3007 12277-12284 RB denotes Finally
T3008 12289-12291 VBZ denotes is
T3009 12284-12286 , denotes ,
T3010 12286-12288 PRP denotes it
T3011 12292-12301 JJ denotes important
T3012 12302-12304 TO denotes to
T3013 12305-12309 VB denotes note
T3014 12310-12314 IN denotes that
T3015 12396-12399 VBP denotes are
T3016 12315-12318 DT denotes the
T3017 12323-12329 NNS denotes models
T3018 12319-12322 CD denotes two
T3019 12330-12333 IN denotes for
T3020 12334-12337 DT denotes the
T3021 12338-12346 NN denotes function
T3022 12347-12349 IN denotes of
T3023 12350-12353 DT denotes the
T3024 12366-12377 NN denotes interaction
T3025 12354-12359 NN denotes BRCA2
T3026 12360-12365 NN denotes RAD51
T3027 12359-12360 HYPH denotes –
T3028 12378-12383 VBN denotes shown
T3029 12384-12386 IN denotes in
T3030 12387-12393 NN denotes Figure
T3031 12394-12395 CD denotes 2
T3032 12400-12403 RB denotes not
T3033 12404-12412 RB denotes mutually
T3034 12413-12422 JJ denotes exclusive
T3035 12422-12423 . denotes .
T3036 12423-12578 sentence denotes BRCA2 could prevent inappropriate RAD51 assembly in the absence of damage and promote functional RAD51 assembly at DNA lesions during the damage response.
T3037 12424-12429 NN denotes BRCA2
T3038 12436-12443 VB denotes prevent
T3039 12430-12435 MD denotes could
T3040 12444-12457 JJ denotes inappropriate
T3041 12464-12472 NN denotes assembly
T3042 12458-12463 NN denotes RAD51
T3043 12473-12475 IN denotes in
T3044 12476-12479 DT denotes the
T3045 12480-12487 NN denotes absence
T3046 12488-12490 IN denotes of
T3047 12491-12497 NN denotes damage
T3048 12498-12501 CC denotes and
T3049 12502-12509 VB denotes promote
T3050 12510-12520 JJ denotes functional
T3051 12527-12535 NN denotes assembly
T3052 12521-12526 NN denotes RAD51
T3053 12536-12538 IN denotes at
T3054 12539-12542 NN denotes DNA
T3055 12543-12550 NNS denotes lesions
T3056 12551-12557 IN denotes during
T3057 12558-12561 DT denotes the
T3058 12569-12577 NN denotes response
T3059 12562-12568 NN denotes damage
T3060 12577-12578 . denotes .
T3061 12578-12713 sentence denotes Further experiments are clearly required to clarify the functional interactions between BRCA2 and RAD51 during recombinational repair.
T3062 12579-12586 JJ denotes Further
T3063 12587-12598 NNS denotes experiments
T3064 12611-12619 VBN denotes required
T3065 12599-12602 VBP denotes are
T3066 12603-12610 RB denotes clearly
T3067 12620-12622 TO denotes to
T3068 12623-12630 VB denotes clarify
T3069 12631-12634 DT denotes the
T3070 12646-12658 NNS denotes interactions
T3071 12635-12645 JJ denotes functional
T3072 12659-12666 IN denotes between
T3073 12667-12672 NN denotes BRCA2
T3074 12673-12676 CC denotes and
T3075 12677-12682 NN denotes RAD51
T3076 12683-12689 IN denotes during
T3077 12690-12705 JJ denotes recombinational
T3078 12706-12712 NN denotes repair
T3079 12712-12713 . denotes .
T3080 12713-12841 sentence denotes It should be relatively straightforward to determine whether the role of BRCA2 is limited to transporting RAD51 to the nucleus.
T3081 12714-12716 PRP denotes It
T3082 12724-12726 VB denotes be
T3083 12717-12723 MD denotes should
T3084 12727-12737 RB denotes relatively
T3085 12738-12753 JJ denotes straightforward
T3086 12754-12756 TO denotes to
T3087 12757-12766 VB denotes determine
T3088 12767-12774 IN denotes whether
T3089 12796-12803 VBN denotes limited
T3090 12775-12778 DT denotes the
T3091 12779-12783 NN denotes role
T3092 12784-12786 IN denotes of
T3093 12787-12792 NN denotes BRCA2
T3094 12793-12795 VBZ denotes is
T3095 12804-12806 IN denotes to
T3096 12807-12819 VBG denotes transporting
T3097 12820-12825 NN denotes RAD51
T3098 12826-12828 IN denotes to
T3099 12829-12832 DT denotes the
T3100 12833-12840 NN denotes nucleus
T3101 12840-12841 . denotes .
T3102 12841-12995 sentence denotes If this were the case, addition of a nuclear localization signal to RAD51 should suppress the RAD51-mediated phenotypes associated with a BRCA2 mutation.
T3103 12842-12844 IN denotes If
T3104 12850-12854 VBD denotes were
T3105 12845-12849 DT denotes this
T3106 12923-12931 VB denotes suppress
T3107 12855-12858 DT denotes the
T3108 12859-12863 NN denotes case
T3109 12863-12865 , denotes ,
T3110 12865-12873 NN denotes addition
T3111 12874-12876 IN denotes of
T3112 12877-12878 DT denotes a
T3113 12900-12906 NN denotes signal
T3114 12879-12886 JJ denotes nuclear
T3115 12887-12899 NN denotes localization
T3116 12907-12909 IN denotes to
T3117 12910-12915 NN denotes RAD51
T3118 12916-12922 MD denotes should
T3119 12932-12935 DT denotes the
T3120 12951-12961 NNS denotes phenotypes
T3121 12936-12941 NN denotes RAD51
T3122 12942-12950 VBN denotes mediated
T3123 12941-12942 HYPH denotes -
T3124 12962-12972 VBN denotes associated
T3125 12973-12977 IN denotes with
T3126 12978-12979 DT denotes a
T3127 12986-12994 NN denotes mutation
T3128 12980-12985 NN denotes BRCA2
T3129 12994-12995 . denotes .
T3130 12995-13122 sentence denotes However, biochemical experiments are needed to determine whether full-length BRCA2 blocks or promotes RAD51–BRCA2 interaction.
T3131 12996-13003 RB denotes However
T3132 13033-13039 VBN denotes needed
T3133 13003-13005 , denotes ,
T3134 13005-13016 JJ denotes biochemical
T3135 13017-13028 NNS denotes experiments
T3136 13029-13032 VBP denotes are
T3137 13040-13042 TO denotes to
T3138 13043-13052 VB denotes determine
T3139 13053-13060 IN denotes whether
T3140 13079-13085 VBZ denotes blocks
T3141 13061-13065 JJ denotes full
T3142 13066-13072 NN denotes length
T3143 13065-13066 HYPH denotes -
T3144 13073-13078 NN denotes BRCA2
T3145 13086-13088 CC denotes or
T3146 13089-13097 VBZ denotes promotes
T3147 13098-13103 NN denotes RAD51
T3148 13104-13109 NN denotes BRCA2
T3149 13103-13104 HYPH denotes –
T3150 13110-13121 NN denotes interaction
T3151 13121-13122 . denotes .
T3152 13122-13211 sentence denotes Such experiments could also reveal additional roles for BRCA2 in recombinational repair.
T3153 13123-13127 JJ denotes Such
T3154 13128-13139 NNS denotes experiments
T3155 13151-13157 VB denotes reveal
T3156 13140-13145 MD denotes could
T3157 13146-13150 RB denotes also
T3158 13158-13168 JJ denotes additional
T3159 13169-13174 NNS denotes roles
T3160 13175-13178 IN denotes for
T3161 13179-13184 NN denotes BRCA2
T3162 13185-13187 IN denotes in
T3163 13188-13203 JJ denotes recombinational
T3164 13204-13210 NN denotes repair
T3165 13210-13211 . denotes .
T3166 13211-13358 sentence denotes However, several technical obstacles, including the large size of BRCA2, promise to make biochemical characterization of BRCA2 function difficult.
T3167 13212-13219 RB denotes However
T3168 13285-13292 VBP denotes promise
T3169 13219-13221 , denotes ,
T3170 13221-13228 JJ denotes several
T3171 13239-13248 NNS denotes obstacles
T3172 13229-13238 JJ denotes technical
T3173 13248-13250 , denotes ,
T3174 13250-13259 VBG denotes including
T3175 13260-13263 DT denotes the
T3176 13270-13274 NN denotes size
T3177 13264-13269 JJ denotes large
T3178 13275-13277 IN denotes of
T3179 13278-13283 NN denotes BRCA2
T3180 13283-13285 , denotes ,
T3181 13293-13295 TO denotes to
T3182 13296-13300 VB denotes make
T3183 13301-13312 JJ denotes biochemical
T3184 13313-13329 NN denotes characterization
T3185 13348-13357 JJ denotes difficult
T3186 13330-13332 IN denotes of
T3187 13333-13338 NN denotes BRCA2
T3188 13339-13347 NN denotes function
T3189 13357-13358 . denotes .
R154 T247 T246 prep from,data
R155 T248 T247 pobj families,from
R156 T249 T248 prep with,families
R157 T250 T251 amod inherited,cancer
R158 T251 T249 pobj cancer,with
R159 T252 T251 compound breast,cancer
R160 T253 T254 punct [,4
R161 T254 T241 parataxis 4,isolated
R162 T255 T254 punct ],4
R163 T256 T228 punct .,was
R164 T258 T259 nsubj Cells,are
R165 T260 T258 prep with,Cells
R166 T261 T262 amod mutant,protein
R167 T262 T260 pobj protein,with
R168 T263 T262 compound BRCA2,protein
R169 T264 T259 punct ", ",are
R170 T265 T259 prep like,are
R171 T266 T267 amod many,cells
R172 T267 T265 pobj cells,like
R173 T268 T267 compound cancer,cells
R174 T269 T259 punct ", ",are
R175 T270 T271 advmod genetically,unstable
R176 T271 T259 acomp unstable,are
R177 T272 T259 cc and,are
R178 T273 T259 conj accumulate,are
R179 T274 T275 amod gross,rearrangements
R180 T275 T273 dobj rearrangements,accumulate
R181 T276 T275 amod chromosomal,rearrangements
R182 T277 T278 punct [,6
R183 T278 T273 parataxis 6,accumulate
R184 T279 T278 nummod 5,6
R185 T280 T278 punct ",",6
R186 T281 T278 punct ],6
R187 T282 T259 punct .,are
R188 T284 T285 det The,sequence
R189 T285 T286 nsubj sequence,offers
R190 T287 T285 prep of,sequence
R191 T288 T289 det this,protein
R192 T289 T287 pobj protein,of
R193 T290 T289 amod large,protein
R194 T291 T285 punct (,sequence
R195 T292 T293 nummod 3418,acids
R207 T304 T286 advcl are,offers
R208 T305 T304 expl there,are
R209 T306 T307 nummod eight,segments
R210 T307 T304 attr segments,are
R211 T308 T307 amod repeated,segments
R212 T309 T310 punct (,termed
R213 T310 T307 parataxis termed,segments
R214 T311 T312 compound BRC,repeats
R215 T312 T310 oprd repeats,termed
R216 T313 T310 punct ),termed
R217 T314 T307 prep in,segments
R218 T315 T316 det the,middle
R219 T316 T314 pobj middle,in
R220 T317 T316 prep of,middle
R221 T318 T319 det the,protein
R222 T319 T317 pobj protein,of
R223 T320 T321 dep that,are
R224 T321 T307 relcl are,segments
R225 T322 T323 advmod highly,conserved
R226 T323 T321 acomp conserved,are
R227 T324 T321 prep among,are
R228 T325 T326 amod mammalian,orthologs
R229 T326 T324 pobj orthologs,among
R230 T327 T328 punct [,8
R231 T328 T304 parataxis 8,are
R232 T329 T328 nummod 7,8
R233 T330 T328 punct ",",8
R234 T331 T328 punct ],8
R235 T332 T286 punct .,offers
R241 T437 T438 prep Following,provided
R242 T439 T440 det the,discovery
R243 T440 T437 pobj discovery,Following
R244 T441 T440 compound landmark,discovery
R245 T442 T440 prep by,discovery
R246 T443 T442 pobj Scully,by
R247 T444 T445 advmod et,al
R248 T445 T443 advmod al,Scully
R249 T446 T447 mark that,colocalizes
R250 T447 T440 acl colocalizes,discovery
R251 T448 T449 det the,RAD51
R252 T449 T447 nsubj RAD51,colocalizes
R253 T450 T449 amod homologous,RAD51
R254 T451 T449 compound recombinase,RAD51
R255 T452 T447 prep at,colocalizes
R256 T453 T454 amod subnuclear,sites
R257 T454 T452 pobj sites,at
R258 T455 T447 prep with,colocalizes
R259 T456 T455 pobj BRCA1,with
R260 T457 T458 punct [,9
R261 T458 T447 parataxis 9,colocalizes
R262 T459 T458 punct ],9
R263 T460 T438 punct ", ",provided
R264 T461 T462 det a,number
R265 T462 T438 nsubj number,provided
R266 T463 T462 prep of,number
R267 T464 T465 amod additional,results
R268 T465 T463 pobj results,of
R269 T466 T438 aux have,provided
R270 T467 T438 dobj evidence,provided
R271 T468 T469 mark that,involved
R272 T469 T467 acl involved,evidence
R273 T470 T471 preconj both,BRCA1
R274 T471 T469 nsubjpass BRCA1,involved
R275 T472 T471 cc and,BRCA1
R276 T473 T471 conj BRCA2,BRCA1
R277 T474 T469 auxpass are,involved
R278 T475 T469 prep in,involved
R279 T476 T477 amod recombinational,repair
R280 T477 T475 pobj repair,in
R281 T478 T477 prep of,repair
R282 T479 T480 compound DNA,damage
R283 T480 T478 pobj damage,of
R284 T481 T438 punct .,provided
R285 T483 T484 nsubj BRCA1,form
R286 T485 T483 cc and,BRCA1
R287 T486 T483 conj BRCA2,BRCA1
R288 T487 T488 amod discrete,foci
R289 T488 T484 dobj foci,form
R290 T489 T488 amod nuclear,foci
R291 T490 T484 prep during,form
R292 T491 T492 det the,phase
R293 T492 T490 pobj phase,during
R294 T493 T492 compound S,phase
R295 T494 T490 cc and,during
R296 T495 T490 conj after,during
R297 T496 T495 pobj exposure,after
R298 T497 T496 prep to,exposure
R299 T498 T499 npadvmod DNA,damaging
R300 T499 T500 amod damaging,agents
R301 T500 T497 pobj agents,to
R302 T501 T502 punct [,11
R303 T502 T484 parataxis 11,form
R304 T503 T502 nummod 9,11
R305 T504 T502 punct ",",11
R306 T505 T502 nummod 10,11
R307 T506 T502 punct ",",11
R308 T507 T502 punct ],11
R309 T508 T484 punct .,form
R310 T510 T511 det These,foci
R311 T511 T512 nsubj foci,are
R312 T513 T512 advmod probably,are
R313 T514 T512 attr sites,are
R314 T515 T514 prep of,sites
R315 T516 T515 pobj repair,of
R316 T517 T516 prep of,repair
R317 T518 T519 amod spontaneous,damage
R318 T519 T517 pobj damage,of
R319 T520 T518 cc and,spontaneous
R320 T521 T518 conj induced,spontaneous
R321 T522 T519 compound DNA,damage
R322 T523 T524 punct [,14
R323 T524 T512 parataxis 14,are
R324 T525 T524 nummod 12,14
R325 T526 T524 punct ",",14
R326 T527 T524 nummod 13,14
R327 T528 T524 punct ",",14
R328 T529 T524 punct ],14
R329 T530 T512 punct .,are
R330 T532 T533 compound Cell,lines
R331 T533 T534 nsubj lines,are
R332 T535 T533 amod defective,lines
R333 T536 T535 prep in,defective
R334 T537 T538 preconj either,BRCA1
R335 T538 T536 pobj BRCA1,in
R336 T539 T538 cc or,BRCA1
R337 T540 T538 conj BRCA2,BRCA1
R338 T541 T534 acomp sensitive,are
R339 T542 T541 prep to,sensitive
R340 T543 T544 amod damaging,agents
R341 T544 T542 pobj agents,to
R342 T545 T546 dep that,form
R343 T546 T544 relcl form,agents
R344 T547 T548 amod double,strand
R345 T548 T550 amod strand,breaks
R346 T549 T548 punct -,strand
R347 T550 T546 dobj breaks,form
R348 T551 T550 punct (,breaks
R349 T552 T550 appos DSBs,breaks
R350 T553 T534 punct ),are
R351 T554 T534 punct ", ",are
R352 T555 T556 mark as,are
R353 T556 T534 advcl are,are
R354 T557 T558 amod other,lines
R355 T558 T556 nsubj lines,are
R356 T559 T558 compound cell,lines
R357 T560 T558 amod defective,lines
R358 T561 T560 prep in,defective
R359 T562 T563 amod recombinational,repair
R360 T563 T561 pobj repair,in
R361 T564 T565 punct (,reviewed
R362 T565 T534 parataxis reviewed,are
R363 T566 T565 prep in,reviewed
R364 T567 T566 punct [,in
R365 T568 T566 pobj 15,in
R366 T569 T566 punct ],in
R367 T570 T565 punct ),reviewed
R368 T571 T534 punct .,are
R369 T573 T574 nsubj BRCA2,interacts
R370 T575 T574 prep with,interacts
R371 T576 T577 det the,recombinase
R372 T577 T575 pobj recombinase,with
R373 T578 T577 compound RAD51,recombinase
R374 T579 T574 prep via,interacts
R375 T580 T581 amod direct,contacts
R376 T581 T579 pobj contacts,via
R377 T582 T583 compound protein,protein
R378 T583 T581 compound protein,contacts
R379 T584 T583 punct -,protein
R380 T585 T586 punct [,19
R381 T586 T574 parataxis 19,interacts
R382 T587 T586 nummod 16,19
R383 T588 T586 punct ",",19
R384 T589 T586 nummod 17,19
R385 T590 T586 punct ",",19
R386 T591 T586 nummod 18,19
R387 T592 T586 punct ",",19
R388 T593 T586 punct ],19
R389 T594 T574 punct .,interacts
R390 T596 T597 amod Biochemical,analysis
R391 T597 T598 nsubj analysis,showed
R392 T599 T598 advmod also,showed
R393 T600 T598 dobj interaction,showed
R394 T601 T600 prep between,interaction
R395 T602 T601 pobj BRCA1,between
R396 T603 T602 cc and,BRCA1
R397 T604 T602 conj RAD51,BRCA1
R398 T605 T598 punct ", ",showed
R399 T606 T607 mark although,been
R400 T607 T598 advcl been,showed
R401 T608 T609 det these,interactions
R402 T609 T607 nsubj interactions,been
R403 T610 T609 amod detected,interactions
R404 T611 T607 aux may,been
R405 T612 T607 aux have,been
R406 T613 T607 acomp indirect,been
R407 T614 T615 punct [,9
R408 T615 T598 parataxis 9,showed
R409 T616 T615 punct ],9
R410 T617 T598 punct .,showed
R411 T619 T620 det The,repeats
R412 T620 T622 nsubj repeats,are
R413 T621 T620 compound BRC,repeats
R414 T623 T620 prep of,repeats
R415 T624 T623 pobj BRCA2,of
R416 T625 T622 acomp responsible,are
R417 T626 T625 prep for,responsible
R418 T627 T628 amod direct,interaction
R419 T628 T626 pobj interaction,for
R420 T629 T628 compound RAD51,interaction
R421 T630 T622 punct .,are
R422 T632 T633 nsubj Cells,fail
R423 T634 T632 acl lacking,Cells
R424 T635 T636 compound BRCA1,2
R425 T636 T634 dobj 2,lacking
R426 T637 T636 punct /,2
R427 T638 T639 aux to,form
R428 T639 T633 xcomp form,fail
R429 T640 T641 npadvmod damage,induced
R430 T641 T643 amod induced,RAD51
R431 T642 T641 punct -,induced
R432 T643 T645 compound RAD51,foci
R433 T644 T643 amod subnuclear,RAD51
R434 T645 T639 dobj foci,form
R435 T646 T639 prep with,form
R436 T647 T648 amod normal,efficiency
R437 T648 T646 pobj efficiency,with
R438 T649 T633 punct ", ",fail
R439 T650 T633 advcl suggesting,fail
R440 T651 T652 mark that,required
R441 T652 T650 ccomp required,suggesting
R442 T653 T654 det these,proteins
R443 T654 T652 nsubjpass proteins,required
R444 T655 T652 auxpass are,required
R445 T656 T652 prep for,required
R446 T657 T658 det the,formation
R447 T658 T656 pobj formation,for
R448 T659 T658 prep of,formation
R449 T660 T661 compound recombinase,complexes
R450 T661 T659 pobj complexes,of
R451 T662 T658 prep at,formation
R452 T663 T664 det the,sites
R453 T664 T662 pobj sites,at
R454 T665 T664 prep of,sites
R455 T666 T667 compound DNA,damage
R456 T667 T665 pobj damage,of
R457 T668 T669 punct [,21
R458 T669 T633 parataxis 21,fail
R459 T670 T669 nummod 20,21
R460 T671 T669 punct ",",21
R461 T672 T669 punct ],21
R462 T673 T633 punct .,fail
R463 T675 T676 advmod Finally,shown
R464 T677 T676 punct ", ",shown
R465 T678 T679 amod genetic,measurements
R466 T679 T676 nsubj measurements,shown
R467 T680 T679 prep of,measurements
R468 T681 T682 compound recombination,frequency
R469 T682 T680 pobj frequency,of
R470 T683 T676 aux have,shown
R471 T684 T685 mark that,are
R472 T685 T676 ccomp are,shown
R473 T686 T687 nmod Brca1,cells
R474 T687 T685 nsubj cells,are
R475 T688 T686 punct -,Brca1
R476 T689 T686 punct /,Brca1
R477 T690 T686 punct -,Brca1
R478 T691 T692 amod embryonic,stem
R479 T692 T687 nmod stem,cells
R480 T693 T692 punct (,stem
R481 T694 T692 appos ES,stem
R482 T695 T687 punct ),cells
R483 T696 T685 acomp deficient,are
R484 T697 T696 prep in,deficient
R485 T698 T699 amod recombinational,repair
R486 T699 T697 pobj repair,in
R487 T700 T699 compound DSB,repair
R488 T701 T702 punct [,23
R489 T702 T676 parataxis 23,shown
R490 T703 T702 nummod 22,23
R491 T704 T702 punct ",",23
R492 T705 T702 punct ],23
R493 T706 T676 punct .,shown
R494 T708 T709 det The,similarity
R495 T709 T710 nsubj similarity,led
R496 T711 T709 prep between,similarity
R497 T712 T711 pobj phenotypes,between
R498 T713 T712 acl associated,phenotypes
R499 T714 T713 prep with,associated
R500 T715 T716 nmod BRCA1,deficiency
R501 T716 T714 pobj deficiency,with
R502 T717 T715 cc and,BRCA1
R503 T718 T715 conj BRCA2,BRCA1
R504 T719 T709 punct ", ",similarity
R505 T720 T721 advmod together,with
R506 T721 T709 prep with,similarity
R507 T722 T721 pobj data,with
R508 T723 T722 acl showing,data
R509 T724 T725 det a,effect
R510 T725 T723 dobj effect,showing
R511 T726 T725 amod similar,effect
R512 T727 T725 prep of,effect
R513 T728 T729 compound DNA,damage
R514 T729 T727 pobj damage,of
R515 T730 T729 prep on,damage
R516 T731 T730 pobj distribution,on
R517 T732 T731 prep of,distribution
R518 T733 T732 pobj BRCA1,of
R519 T734 T733 cc and,BRCA1
R520 T735 T733 conj BRCA2,BRCA1
R521 T736 T731 prep in,distribution
R522 T737 T738 npadvmod repair,proficient
R523 T738 T740 amod proficient,cells
R524 T739 T738 punct -,proficient
R525 T740 T736 pobj cells,in
R526 T741 T710 punct ", ",led
R527 T742 T710 prep to,led
R528 T743 T744 det the,hypothesis
R529 T744 T742 pobj hypothesis,to
R530 T745 T746 mark that,required
R531 T746 T744 acl required,hypothesis
R532 T747 T746 nsubjpass BRCA2,required
R533 T748 T747 punct ", ",BRCA2
R534 T749 T747 prep like,BRCA2
R535 T750 T749 pobj BRCA1,like
R536 T751 T746 punct ", ",required
R537 T752 T746 auxpass is,required
R538 T753 T746 prep for,required
R539 T754 T755 amod efficient,repair
R540 T755 T753 pobj repair,for
R541 T756 T755 amod recombinational,repair
R542 T757 T710 punct .,led
R543 T759 T760 det The,paper
R544 T760 T761 nsubj paper,provides
R545 T762 T760 prep by,paper
R546 T763 T762 pobj Moynahan,by
R547 T764 T765 advmod et,al
R548 T765 T763 advmod al,Moynahan
R549 T766 T767 punct [,24
R550 T767 T760 parataxis 24,paper
R551 T768 T767 punct ],24
R552 T769 T770 amod important,support
R553 T770 T761 dobj support,provides
R554 T771 T761 dative for,provides
R555 T772 T773 det this,hypothesis
R556 T773 T771 pobj hypothesis,for
R557 T774 T761 punct .,provides
R564 T1002 T1003 nsubj Pierce,designed
R565 T1004 T1005 advmod et,al
R566 T1005 T1002 advmod al,Pierce
R567 T1006 T1003 aux have,designed
R568 T1007 T1008 det a,set
R569 T1008 T1003 dobj set,designed
R570 T1009 T1008 prep of,set
R571 T1010 T1011 compound recombination,substrates
R572 T1011 T1009 pobj substrates,of
R573 T1012 T1003 prep for,designed
R574 T1013 T1012 pcomp measuring,for
R575 T1014 T1015 det the,level
R576 T1015 T1013 dobj level,measuring
R577 T1016 T1015 prep of,level
R578 T1017 T1018 amod homologous,recombination
R579 T1018 T1016 pobj recombination,of
R580 T1019 T1020 advmod in,vivo
R581 T1020 T1013 advmod vivo,measuring
R582 T1021 T1022 punct (,Fig.
R583 T1022 T1003 parataxis Fig.,designed
R584 T1023 T1022 nummod 1,Fig.
R585 T1024 T1022 punct ),Fig.
R586 T1025 T1026 punct [,25
R587 T1026 T1003 parataxis 25,designed
R588 T1027 T1026 punct ],25
R589 T1028 T1003 punct .,designed
R590 T1030 T1031 det The,substrate
R591 T1031 T1033 nsubj substrate,contains
R592 T1032 T1031 compound DNA,substrate
R593 T1034 T1035 det a,pair
R594 T1035 T1033 dobj pair,contains
R595 T1036 T1035 prep of,pair
R596 T1037 T1038 amod mutated,genes
R597 T1038 T1036 pobj genes,of
R598 T1039 T1038 compound GFP,genes
R599 T1040 T1041 punct (,encodes
R600 T1041 T1035 parataxis encodes,pair
R601 T1042 T1041 nsubj GFP,encodes
R602 T1043 T1044 det the,protein
R603 T1044 T1041 dobj protein,encodes
R604 T1045 T1046 advmod easily,detected
R605 T1046 T1044 amod detected,protein
R606 T1047 T1044 amod green,protein
R607 T1048 T1044 amod fluorescent,protein
R608 T1049 T1041 punct ),encodes
R609 T1050 T1035 punct ", ",pair
R610 T1051 T1052 dep one,contains
R611 T1052 T1035 relcl contains,pair
R612 T1053 T1051 prep of,one
R613 T1054 T1053 pobj which,of
R614 T1055 T1056 det a,site
R615 T1056 T1052 dobj site,contains
R616 T1057 T1056 compound restriction,site
R617 T1058 T1056 prep for,site
R618 T1059 T1060 compound I,SceI
R619 T1060 T1058 pobj SceI,for
R620 T1061 T1060 punct -,SceI
R621 T1062 T1060 punct ", ",SceI
R622 T1063 T1064 det a,endonuclease
R623 T1064 T1060 appos endonuclease,SceI
R624 T1065 T1066 compound yeast,intron
R625 T1066 T1067 npadvmod intron,encoded
R626 T1067 T1064 amod encoded,endonuclease
R627 T1068 T1064 prep with,endonuclease
R628 T1069 T1070 det an,site
R629 T1070 T1068 pobj site,with
R630 T1071 T1072 nummod 18,pair
R631 T1072 T1070 compound pair,site
R632 T1073 T1072 compound base,pair
R633 T1074 T1070 compound recognition,site
R634 T1075 T1033 punct .,contains
R635 T1077 T1078 amod Transient,transfection
R636 T1078 T1079 nsubj transfection,results
R637 T1080 T1078 prep of,transfection
R638 T1081 T1082 det an,vector
R639 T1082 T1080 pobj vector,of
R640 T1083 T1084 compound I,SceI
R641 T1084 T1082 compound SceI,vector
R642 T1085 T1084 punct -,SceI
R643 T1086 T1082 compound expression,vector
R644 T1087 T1079 prep in,results
R645 T1088 T1089 det the,production
R646 T1089 T1087 pobj production,in
R647 T1090 T1089 prep of,production
R648 T1091 T1092 det a,DSB
R649 T1092 T1090 pobj DSB,of
R650 T1093 T1089 prep in,production
R651 T1094 T1095 det the,copy
R652 T1095 T1093 pobj copy,in
R653 T1096 T1095 amod first,copy
R654 T1097 T1095 amod mutated,copy
R655 T1098 T1095 prep of,copy
R656 T1099 T1098 pobj GFP,of
R657 T1100 T1079 punct .,results
R658 T1102 T1103 nsubj One,invade
R659 T1104 T1102 cc or,One
R660 T1105 T1106 nmod both,ends
R661 T1106 T1102 conj ends,One
R662 T1107 T1106 compound DNA,ends
R663 T1108 T1102 acl formed,One
R664 T1109 T1108 agent by,formed
R665 T1110 T1111 det the,break
R666 T1111 T1109 pobj break,by
R667 T1112 T1103 punct (,invade
R668 T1113 T1103 dep s,invade
R669 T1114 T1103 punct ),invade
R670 T1115 T1116 det the,sequence
R671 T1116 T1103 dobj sequence,invade
R672 T1117 T1116 amod homologous,sequence
R673 T1118 T1116 prep in,sequence
R674 T1119 T1120 det the,copy
R675 T1120 T1118 pobj copy,in
R676 T1121 T1120 amod second,copy
R677 T1122 T1123 amod mutant,GFP
R678 T1123 T1120 compound GFP,copy
R679 T1124 T1103 punct ", ",invade
R680 T1125 T1103 advcl resulting,invade
R681 T1126 T1125 prep in,resulting
R682 T1127 T1126 pobj repair,in
R683 T1128 T1127 prep of,repair
R684 T1129 T1130 det the,DSB
R685 T1130 T1128 pobj DSB,of
R686 T1131 T1127 prep via,repair
R687 T1132 T1133 det a,event
R688 T1133 T1131 pobj event,via
R689 T1134 T1135 npadvmod homology,mediated
R690 T1135 T1133 amod mediated,event
R691 T1136 T1135 punct -,mediated
R692 T1137 T1138 compound gene,conversion
R693 T1138 T1133 compound conversion,event
R694 T1139 T1103 punct .,invade
R695 T1141 T1142 det The,configuration
R696 T1142 T1143 nsubj configuration,is
R697 T1144 T1142 prep of,configuration
R698 T1145 T1146 det the,construct
R699 T1146 T1144 pobj construct,of
R700 T1147 T1146 compound GFP,construct
R701 T1148 T1143 acomp such,is
R702 T1149 T1150 mark that,results
R703 T1150 T1148 ccomp results,such
R704 T1151 T1152 npadvmod homology,mediated
R705 T1152 T1154 amod mediated,repair
R706 T1153 T1152 punct -,mediated
R707 T1154 T1150 nsubj repair,results
R708 T1155 T1150 advmod often,results
R709 T1156 T1150 prep in,results
R710 T1157 T1158 det the,formation
R711 T1158 T1156 pobj formation,in
R712 T1159 T1158 prep of,formation
R713 T1160 T1161 det a,copy
R714 T1161 T1159 pobj copy,of
R715 T1162 T1161 amod functional,copy
R716 T1163 T1161 prep of,copy
R717 T1164 T1163 pobj GFP,of
R718 T1165 T1143 punct .,is
R719 T1167 T1168 amod Such,events
R720 T1168 T1169 nsubjpass events,detected
R721 T1170 T1169 aux can,detected
R722 T1171 T1169 auxpass be,detected
R723 T1172 T1169 prep by,detected
R724 T1173 T1174 npadvmod fluorescence,activated
R725 T1174 T1176 amod activated,sorting
R726 T1175 T1174 punct -,activated
R727 T1176 T1178 compound sorting,analysis
R728 T1177 T1176 compound cell,sorting
R729 T1178 T1172 pobj analysis,by
R730 T1179 T1169 prep by,detected
R731 T1180 T1179 pobj virtue,by
R732 T1181 T1180 prep of,virtue
R733 T1182 T1183 poss their,expression
R734 T1183 T1181 pobj expression,of
R735 T1184 T1183 prep of,expression
R736 T1185 T1184 pobj GFP,of
R737 T1186 T1169 punct .,detected
R738 T1188 T1189 nsubj Moynahan,used
R739 T1190 T1191 advmod et,al
R740 T1191 T1188 advmod al,Moynahan
R741 T1192 T1193 punct [,24
R742 T1193 T1188 parataxis 24,Moynahan
R743 T1194 T1193 punct ],24
R744 T1195 T1189 aux have,used
R745 T1196 T1197 predet such,substrate
R746 T1197 T1189 dobj substrate,used
R747 T1198 T1197 det a,substrate
R748 T1199 T1200 compound GFP,recombination
R749 T1200 T1197 compound recombination,substrate
R750 T1201 T1202 aux to,demonstrate
R751 T1202 T1189 advcl demonstrate,used
R752 T1203 T1204 mark that,are
R753 T1204 T1202 ccomp are,demonstrate
R754 T1205 T1204 nsubj cells,are
R755 T1206 T1205 prep with,cells
R756 T1207 T1208 amod defective,protein
R757 T1208 T1206 pobj protein,with
R758 T1209 T1208 compound BRCA2,protein
R759 T1210 T1204 acomp deficient,are
R760 T1211 T1210 prep in,deficient
R761 T1212 T1213 poss their,ability
R762 T1213 T1211 pobj ability,in
R763 T1214 T1215 aux to,repair
R764 T1215 T1213 acl repair,ability
R765 T1216 T1217 det the,DSB
R766 T1217 T1215 dobj DSB,repair
R767 T1218 T1219 compound I,SceI
R768 T1219 T1221 npadvmod SceI,induced
R769 T1220 T1219 punct -,SceI
R770 T1221 T1217 amod induced,DSB
R771 T1222 T1221 punct -,induced
R772 T1223 T1215 prep through,repair
R773 T1224 T1225 amod homologous,recombination
R774 T1225 T1223 pobj recombination,through
R775 T1226 T1189 punct .,used
R776 T1228 T1229 nsubj Expression,resulted
R777 T1230 T1228 prep of,Expression
R778 T1231 T1232 compound I,SceI
R779 T1232 T1230 pobj SceI,of
R780 T1233 T1232 punct -,SceI
R781 T1234 T1229 prep in,resulted
R782 T1235 T1236 quantmod 1,1400
R783 T1236 T1239 nummod 1400,cells
R784 T1237 T1236 quantmod out,1400
R785 T1238 T1236 quantmod of,1400
R786 T1239 T1240 nsubj cells,producing
R787 T1240 T1234 pcomp producing,in
R788 T1241 T1240 dobj GFP,producing
R789 T1242 T1240 prep via,producing
R790 T1243 T1244 amod homologous,recombination
R791 T1244 T1242 pobj recombination,via
R792 T1245 T1244 prep in,recombination
R793 T1246 T1247 det the,line
R794 T1247 T1245 pobj line,in
R795 T1248 T1249 amod human,cell
R796 T1249 T1247 compound cell,line
R797 T1250 T1249 amod pancreatic,cell
R798 T1251 T1249 compound tumor,cell
R799 T1252 T1247 appos CAPAN,line
R800 T1253 T1252 punct -,CAPAN
R801 T1254 T1252 nummod 1,CAPAN
R802 T1255 T1229 punct .,resulted
R803 T1257 T1258 nmod CAPAN,cells
R804 T1258 T1261 nsubj cells,carry
R805 T1259 T1257 punct -,CAPAN
R806 T1260 T1257 nummod 1,CAPAN
R807 T1262 T1263 det a,deletion
R808 T1263 T1261 dobj deletion,carry
R809 T1264 T1263 prep of,deletion
R810 T1265 T1264 pobj BRCA2,of
R811 T1266 T1261 prep on,carry
R812 T1267 T1268 nummod one,homolog
R813 T1268 T1266 pobj homolog,on
R814 T1269 T1261 cc and,carry
R815 T1270 T1261 conj codes,carry
R816 T1271 T1270 prep for,codes
R817 T1272 T1273 det a,protein
R818 T1273 T1271 pobj protein,for
R819 T1274 T1273 acl truncated,protein
R820 T1275 T1274 prep at,truncated
R821 T1276 T1277 amod amino,acid
R822 T1277 T1275 pobj acid,at
R823 T1278 T1277 appos 1981,acid
R824 T1279 T1270 prep on,codes
R825 T1280 T1281 det the,homolog
R826 T1281 T1279 pobj homolog,on
R827 T1282 T1281 amod other,homolog
R828 T1283 T1261 punct .,carry
R829 T1285 T1286 det The,authors
R830 T1286 T1287 nsubj authors,indicate
R831 T1288 T1289 mark that,is
R832 T1289 T1287 ccomp is,indicate
R833 T1290 T1291 det the,level
R834 T1291 T1289 nsubj level,is
R835 T1292 T1291 prep of,level
R836 T1293 T1294 compound I,SceI
R837 T1294 T1296 npadvmod SceI,induced
R838 T1295 T1294 punct -,SceI
R839 T1296 T1298 amod induced,recombination
R840 T1297 T1296 punct -,induced
R841 T1298 T1292 pobj recombination,of
R842 T1299 T1291 prep in,level
R843 T1300 T1299 pobj CAPAN,in
R844 T1301 T1300 punct -,CAPAN
R845 T1302 T1300 nummod 1,CAPAN
R846 T1303 T1304 quantmod over,100-fold
R847 T1304 T1305 advmod 100-fold,less
R848 T1305 T1289 acomp less,is
R849 T1306 T1305 prep than,less
R850 T1307 T1306 pobj that,than
R851 T1308 T1307 acl seen,that
R852 T1309 T1308 advcl using,seen
R853 T1310 T1311 amod other,BRCA2+
R854 T1311 T1313 amod BRCA2+,lines
R855 T1312 T1311 punct (,BRCA2+
R856 T1313 T1309 dobj lines,using
R857 T1314 T1313 punct ),lines
R858 T1315 T1316 amod human,tumor
R859 T1316 T1317 compound tumor,cell
R860 T1317 T1313 compound cell,lines
R861 T1318 T1287 punct .,indicate
R862 T1320 T1321 det The,lines
R863 T1321 T1323 nsubj lines,are
R864 T1322 T1321 amod different,lines
R865 T1324 T1321 acl examined,lines
R866 T1325 T1323 punct ", ",are
R867 T1326 T1323 advmod however,are
R868 T1327 T1323 punct ", ",are
R869 T1328 T1329 advmod very,likely
R870 T1329 T1323 acomp likely,are
R871 T1330 T1331 aux to,differ
R872 T1331 T1329 xcomp differ,likely
R873 T1332 T1331 advmod genetically,differ
R874 T1333 T1331 prep from,differ
R875 T1334 T1335 nmod CAPAN,cells
R876 T1335 T1333 pobj cells,from
R877 T1336 T1334 punct -,CAPAN
R878 T1337 T1334 nummod 1,CAPAN
R879 T1338 T1339 preconj not,at
R880 T1339 T1331 prep at,differ
R881 T1340 T1338 advmod only,not
R882 T1341 T1342 det the,locus
R883 T1342 T1339 pobj locus,at
R884 T1343 T1342 compound BRCA2,locus
R885 T1344 T1339 punct ", ",at
R886 T1345 T1339 cc but,at
R887 T1346 T1345 advmod also,but
R888 T1347 T1339 conj at,at
R889 T1348 T1349 det a,number
R890 T1349 T1347 pobj number,at
R891 T1350 T1351 advmod very,large
R892 T1351 T1349 amod large,number
R893 T1352 T1349 prep of,number
R894 T1353 T1354 amod additional,loci
R895 T1354 T1352 pobj loci,of
R896 T1355 T1323 punct .,are
R897 T1357 T1358 nsubj This,raises
R898 T1359 T1360 det the,possibility
R899 T1360 T1358 dobj possibility,raises
R900 T1361 T1362 mark that,be
R901 T1362 T1360 acl be,possibility
R902 T1363 T1362 nsubj some,be
R903 T1364 T1363 cc or,some
R904 T1365 T1366 advmod even,all
R905 T1366 T1363 conj all,some
R906 T1367 T1363 prep of,some
R907 T1368 T1369 det the,defect
R908 T1369 T1367 pobj defect,of
R909 T1370 T1369 amod recombinational,defect
R910 T1371 T1369 compound repair,defect
R911 T1372 T1369 acl seen,defect
R912 T1373 T1372 prep in,seen
R913 T1374 T1373 pobj CAPAN,in
R914 T1375 T1374 punct -,CAPAN
R915 T1376 T1374 nummod 1,CAPAN
R916 T1377 T1362 aux could,be
R917 T1378 T1362 prep due,be
R918 T1379 T1378 pcomp to,due
R919 T1380 T1378 pobj mutations,due
R920 T1381 T1380 prep at,mutations
R921 T1382 T1383 amod non-BRCA2,loci
R922 T1383 T1381 pobj loci,at
R923 T1384 T1358 punct .,raises
R924 T1386 T1387 mark While,were
R925 T1387 T1391 advcl were,argue
R926 T1388 T1387 nsubj Moynahan,were
R927 T1389 T1390 advmod et,al
R928 T1390 T1388 advmod al,Moynahan
R929 T1392 T1387 acomp careful,were
R930 T1393 T1394 aux to,point
R931 T1394 T1392 xcomp point,careful
R932 T1395 T1394 prt out,point
R933 T1396 T1397 det this,problem
R934 T1397 T1394 dobj problem,point
R935 T1398 T1391 punct ", ",argue
R936 T1399 T1400 nummod two,sets
R937 T1400 T1391 nsubj sets,argue
R938 T1401 T1400 prep of,sets
R939 T1402 T1401 pobj results,of
R940 T1403 T1391 prep against,argue
R941 T1404 T1405 det the,possibility
R942 T1405 T1403 pobj possibility,against
R943 T1406 T1407 mark that,is
R944 T1407 T1405 acl is,possibility
R945 T1408 T1409 det the,deficiency
R946 T1409 T1407 nsubj deficiency,is
R947 T1410 T1409 amod recombinational,deficiency
R948 T1411 T1409 compound repair,deficiency
R949 T1412 T1409 prep of,deficiency
R950 T1413 T1414 nmod CAPAN,cells
R951 T1414 T1412 pobj cells,of
R952 T1415 T1413 punct -,CAPAN
R953 T1416 T1413 nummod 1,CAPAN
R954 T1417 T1418 advmod completely,independent
R955 T1418 T1407 acomp independent,is
R956 T1419 T1418 prep of,independent
R957 T1420 T1421 poss its,defect
R958 T1421 T1419 pobj defect,of
R959 T1422 T1421 prep in,defect
R960 T1423 T1422 pobj BRCA2,in
R961 T1424 T1391 punct .,argue
R962 T1426 T1427 advmod First,measured
R963 T1428 T1427 punct ", ",measured
R964 T1429 T1427 prep in,measured
R965 T1430 T1431 det the,study
R966 T1431 T1429 pobj study,in
R967 T1432 T1431 amod same,study
R968 T1433 T1434 punct [,24
R969 T1434 T1431 parataxis 24,study
R970 T1435 T1434 punct ],24
R971 T1436 T1427 punct ", ",measured
R972 T1437 T1427 nsubjpass recombination,measured
R973 T1438 T1437 prep in,recombination
R974 T1439 T1440 det a,line
R975 T1440 T1438 pobj line,in
R976 T1441 T1440 compound mouse,line
R977 T1442 T1440 compound ES,line
R978 T1443 T1440 compound cell,line
R979 T1444 T1445 dep that,expresses
R980 T1445 T1440 relcl expresses,line
R981 T1446 T1447 advmod only,protein
R982 T1447 T1445 dobj protein,expresses
R983 T1448 T1447 amod truncated,protein
R984 T1449 T1447 compound BRCA2,protein
R985 T1450 T1427 auxpass was,measured
R986 T1451 T1427 punct .,measured
R987 T1453 T1454 det This,line
R988 T1454 T1455 nsubjpass line,found
R989 T1456 T1455 auxpass was,found
R990 T1457 T1458 aux to,have
R991 T1458 T1455 xcomp have,found
R992 T1459 T1460 amod lower,efficiency
R993 T1460 T1458 dobj efficiency,have
R994 T1461 T1460 compound recombination,efficiency
R995 T1462 T1460 prep than,efficiency
R996 T1463 T1464 amod isogenic,cells
R997 T1464 T1462 pobj cells,than
R998 T1465 T1464 acl expressing,cells
R999 T1466 T1467 amod full,length
R1000 T1467 T1469 compound length,BRCA2
R1001 T1468 T1467 punct -,length
R1002 T1469 T1465 dobj BRCA2,expressing
R1003 T1470 T1471 punct (,was
R1004 T1471 T1469 parataxis was,BRCA2
R1005 T1472 T1473 det the,defect
R1006 T1473 T1471 nsubj defect,was
R1007 T1474 T1473 acl observed,defect
R1008 T1475 T1476 advmod about,sixfold
R1009 T1476 T1471 advmod sixfold,was
R1010 T1477 T1476 advmod fivefold,sixfold
R1011 T1478 T1476 quantmod to,sixfold
R1012 T1479 T1471 punct ),was
R1013 T1480 T1455 punct .,found
R1014 T1482 T1483 advmod Second,was
R1015 T1484 T1483 punct ", ",was
R1016 T1485 T1483 prep in,was
R1017 T1486 T1487 det an,study
R1018 T1487 T1485 pobj study,in
R1019 T1488 T1487 amod independent,study
R1020 T1489 T1483 punct ", ",was
R1021 T1490 T1483 nsubj Powell,was
R1022 T1491 T1483 acomp able,was
R1023 T1492 T1493 aux to,compare
R1024 T1493 T1491 xcomp compare,able
R1025 T1494 T1493 dobj derivatives,compare
R1026 T1495 T1494 prep of,derivatives
R1027 T1496 T1495 pobj CAPAN,of
R1028 T1497 T1496 punct -,CAPAN
R1029 T1498 T1496 nummod 1,CAPAN
R1030 T1499 T1500 dep that,differed
R1031 T1500 T1494 relcl differed,derivatives
R1032 T1501 T1500 advmod only,differed
R1033 T1502 T1500 prep in,differed
R1034 T1503 T1504 poss their,ability
R1035 T1504 T1502 pobj ability,in
R1036 T1505 T1506 aux to,express
R1037 T1506 T1504 acl express,ability
R1038 T1507 T1508 amod full,length
R1039 T1508 T1510 compound length,protein
R1040 T1509 T1508 punct -,length
R1041 T1510 T1506 dobj protein,express
R1042 T1511 T1510 compound BRCA2,protein
R1043 T1512 T1513 punct (,Powell
R1044 T1513 T1493 meta Powell,compare
R1045 T1514 T1513 compound S.,Powell
R1046 T1515 T1513 punct ", ",Powell
R1047 T1516 T1517 amod personal,communication
R1048 T1517 T1513 dep communication,Powell
R1049 T1518 T1513 punct ", ",Powell
R1050 T1519 T1513 npadvmod 2001,Powell
R1051 T1520 T1513 punct ),Powell
R1052 T1521 T1483 punct .,was
R1053 T1523 T1524 prep In,yielded
R1054 T1525 T1526 det these,experiments
R1055 T1526 T1523 pobj experiments,In
R1056 T1527 T1524 punct ", ",yielded
R1057 T1528 T1529 det the,derivative
R1058 T1529 T1524 nsubj derivative,yielded
R1059 T1530 T1529 acl expressing,derivative
R1060 T1531 T1532 amod full,length
R1061 T1532 T1534 compound length,BRCA2
R1062 T1533 T1532 punct -,length
R1063 T1534 T1530 dobj BRCA2,expressing
R1064 T1535 T1536 advmod 10-fold,more
R1065 T1536 T1537 amod more,recombinants
R1066 T1537 T1524 dobj recombinants,yielded
R1067 T1538 T1537 prep than,recombinants
R1068 T1539 T1538 pobj controls,than
R1069 T1540 T1539 acl expressing,controls
R1070 T1541 T1542 advmod only,BRCA2
R1071 T1542 T1540 dobj BRCA2,expressing
R1072 T1543 T1542 amod truncated,BRCA2
R1073 T1544 T1545 punct (,Powell
R1074 T1545 T1524 meta Powell,yielded
R1075 T1546 T1545 compound S.,Powell
R1076 T1547 T1545 punct ", ",Powell
R1077 T1548 T1549 amod personal,communication
R1078 T1549 T1545 dep communication,Powell
R1079 T1550 T1545 punct ", ",Powell
R1080 T1551 T1545 dep 2001,Powell
R1081 T1552 T1545 punct ),Powell
R1082 T1553 T1524 punct .,yielded
R1083 T1555 T1556 mark While,was
R1084 T1556 T1563 advcl was,raise
R1085 T1557 T1558 det the,design
R1086 T1558 T1556 nsubj design,was
R1087 T1559 T1558 amod experimental,design
R1088 T1560 T1558 prep of,design
R1089 T1561 T1562 det these,experiments
R1090 T1562 T1560 pobj experiments,of
R1091 T1564 T1565 advmod somewhat,different
R1092 T1565 T1556 acomp different,was
R1093 T1566 T1565 prep from,different
R1094 T1567 T1566 pobj that,from
R1095 T1568 T1567 prep of,that
R1096 T1569 T1570 compound Moyna,han
R1097 T1570 T1568 pobj han,of
R1098 T1571 T1570 punct -,han
R1099 T1572 T1573 advmod et,al
R1100 T1573 T1570 advmod al,han
R1101 T1574 T1575 punct [,24
R1102 T1575 T1570 parataxis 24,han
R1103 T1576 T1575 punct ],24
R1104 T1577 T1563 punct ", ",raise
R1105 T1578 T1579 det the,results
R1106 T1579 T1563 nsubj results,raise
R1107 T1580 T1581 det the,possibility
R1108 T1581 T1563 dobj possibility,raise
R1109 T1582 T1583 mark that,have
R1110 T1583 T1581 acl have,possibility
R1111 T1584 T1585 nmod CAPAN,cells
R1112 T1585 T1583 nsubj cells,have
R1113 T1586 T1584 punct -,CAPAN
R1114 T1587 T1584 nummod 1,CAPAN
R1115 T1588 T1589 amod more,one
R1116 T1589 T1591 nummod one,mutation
R1117 T1590 T1589 quantmod than,one
R1118 T1591 T1583 dobj mutation,have
R1119 T1592 T1593 dep that,lowers
R1120 T1593 T1591 relcl lowers,mutation
R1121 T1594 T1595 det the,efficiency
R1122 T1595 T1593 dobj efficiency,lowers
R1123 T1596 T1595 prep of,efficiency
R1124 T1597 T1598 amod recombinational,repair
R1125 T1598 T1596 pobj repair,of
R1126 T1599 T1598 amod relative,repair
R1127 T1600 T1599 prep to,relative
R1128 T1601 T1600 pobj that,to
R1129 T1602 T1601 acl observed,that
R1130 T1603 T1602 prep in,observed
R1131 T1604 T1605 amod other,lines
R1132 T1605 T1603 pobj lines,in
R1133 T1606 T1605 amod human,lines
R1134 T1607 T1605 compound cell,lines
R1135 T1608 T1563 punct .,raise
R1136 T1610 T1611 advmod Conversely,be
R1137 T1612 T1611 punct ", ",be
R1138 T1613 T1614 det the,defect
R1139 T1614 T1611 nsubj defect,be
R1140 T1615 T1614 compound BRCA2,defect
R1141 T1616 T1614 prep in,defect
R1142 T1617 T1616 pobj CAPAN,in
R1143 T1618 T1617 punct -,CAPAN
R1144 T1619 T1617 nummod 1,CAPAN
R1145 T1621 T1622 advmod fully,responsible
R1146 T1622 T1611 acomp responsible,be
R1147 T1623 T1622 prep for,responsible
R1148 T1624 T1625 det the,defect
R1149 T1625 T1623 pobj defect,for
R1150 T1620 T1611 aux could,be
R1151 T1627 T1625 prep in,defect
R1152 T1628 T1627 pobj recombination,in
R1153 T1626 T1625 amod 100-fold,defect
R1154 T1630 T1611 advcl was,be
R1155 T1631 T1632 det the,level
R1156 T1629 T1630 mark if,was
R1157 T1633 T1632 prep of,level
R1158 T1634 T1635 compound BRCA2,complementation
R1159 T1632 T1630 nsubj level,was
R1160 T1636 T1632 acl observed,level
R1161 T1637 T1636 agent by,observed
R1162 T1638 T1637 pobj Powell,by
R1163 T1639 T1630 acomp incomplete,was
R1164 T1635 T1633 pobj complementation,of
R1165 T1642 T1643 advmod Furthermore,accounted
R1166 T1640 T1611 punct .,be
R1167 T1644 T1643 punct ", ",accounted
R1168 T1645 T1646 det the,discrepancy
R1169 T1647 T1646 prep between,discrepancy
R1170 T1648 T1649 det the,difference
R1171 T1646 T1643 nsubjpass discrepancy,accounted
R1172 T1650 T1649 amod 100-fold,difference
R1173 T1651 T1649 prep between,difference
R1174 T1649 T1647 pobj difference,between
R1175 T1653 T1652 punct -,CAPAN
R1176 T1654 T1652 nummod 1,CAPAN
R1177 T1652 T1651 pobj CAPAN,between
R1178 T1656 T1657 det the,lines
R1179 T1657 T1652 conj lines,CAPAN
R1180 T1655 T1652 cc and,CAPAN
R1181 T1659 T1657 amod human,lines
R1182 T1660 T1646 prep as,discrepancy
R1183 T1658 T1657 amod other,lines
R1184 T1662 T1661 prep with,compared
R1185 T1661 T1660 prep compared,as
R1186 T1664 T1662 pobj difference,with
R1187 T1663 T1664 det the,difference
R1188 T1666 T1664 nummod sixfold,difference
R1189 T1665 T1666 amod fivefold,sixfold
R1190 T1668 T1664 prep between,difference
R1191 T1669 T1670 det the,cells
R1192 T1667 T1666 quantmod to,sixfold
R1193 T1671 T1672 compound Brca2lex1,lex2
R1194 T1672 T1670 compound lex2,cells
R1195 T1670 T1668 pobj cells,between
R1196 T1674 T1670 cc and,cells
R1197 T1675 T1676 nmod Brca2,cells
R1198 T1673 T1672 punct /,lex2
R1199 T1677 T1675 punct +,Brca2
R1200 T1678 T1675 punct /,Brca2
R1201 T1679 T1675 punct +,Brca2
R1202 T1676 T1670 conj cells,cells
R1203 T1681 T1643 aux might,accounted
R1204 T1682 T1643 advmod simply,accounted
R1205 T1680 T1676 compound ES,cells
R1206 T1684 T1643 prep for,accounted
R1207 T1685 T1643 prep by,accounted
R1208 T1683 T1643 auxpass be,accounted
R1209 T1687 T1685 pobj fact,by
R1210 T1688 T1689 mark that,is
R1211 T1686 T1687 det the,fact
R1212 T1690 T1691 compound Brca2lex1,lex2
R1213 T1691 T1689 nsubj lex2,is
R1214 T1689 T1687 acl is,fact
R1215 T1693 T1689 neg not,is
R1216 T1694 T1695 det a,allele
R1217 T1692 T1691 punct /,lex2
R1218 T1696 T1695 amod null,allele
R1219 T1697 T1643 punct .,accounted
R1220 T1695 T1689 attr allele,is
R1221 T1699 T1700 amod Additional,studies
R1222 T1700 T1701 nsubj studies,are
R1223 T1702 T1701 acomp likely,are
R1224 T1704 T1702 xcomp shed,likely
R1225 T1705 T1704 dobj light,shed
R1226 T1703 T1704 aux to,shed
R1227 T1707 T1708 det the,efficiency
R1228 T1708 T1706 pobj efficiency,on
R1229 T1706 T1704 prep on,shed
R1230 T1710 T1711 npadvmod BRCA2,independent
R1231 T1711 T1713 amod independent,pathways
R1232 T1709 T1708 prep of,efficiency
R1233 T1713 T1709 pobj pathways,of
R1234 T1714 T1713 compound recombination,pathways
R1235 T1712 T1711 punct -,independent
R1236 T1719 T1717 advmod together,Taken
R1237 T1715 T1701 punct .,are
R1238 T1720 T1718 punct ", ",indicate
R1239 T1717 T1718 advcl Taken,indicate
R1240 T1722 T1718 nsubj results,indicate
R1241 T1721 T1722 det the,results
R1242 T1724 T1718 ccomp required,indicate
R1243 T1725 T1724 nsubjpass BRCA2,required
R1244 T1723 T1724 mark that,required
R1245 T1727 T1724 advmod indeed,required
R1246 T1728 T1724 prep for,required
R1247 T1726 T1724 auxpass is,required
R1248 T1730 T1728 pobj levels,for
R1249 T1729 T1730 amod high,levels
R1250 T1732 T1733 amod recombinational,repair
R1251 T1733 T1731 pobj repair,of
R1252 T1731 T1730 prep of,levels
R1253 T1735 T1736 preconj both,mouse
R1254 T1734 T1724 prep in,required
R1255 T1737 T1736 amod human,mouse
R1256 T1738 T1736 cc and,mouse
R1257 T1736 T1734 pobj mouse,in
R1258 T1739 T1718 punct .,indicate
R1262 T2154 T2155 det The,paper
R1263 T2155 T2157 nsubj paper,is
R1264 T2156 T2155 amod second,paper
R1265 T2158 T2155 acl providing,paper
R1266 T2159 T2158 dobj evidence,providing
R1267 T2160 T2155 punct ", ",paper
R1268 T2161 T2155 prep by,paper
R1269 T2162 T2161 pobj Davies,by
R1270 T2163 T2164 advmod et,al
R1271 T2164 T2162 advmod al,Davies
R1272 T2165 T2166 punct [,26
R1273 T2166 T2155 parataxis 26,paper
R1274 T2167 T2166 punct ],26
R1275 T2168 T2157 punct ", ",is
R1276 T2169 T2170 det a,study
R1277 T2170 T2157 attr study,is
R1278 T2171 T2170 amod biochemical,study
R1279 T2172 T2170 prep of,study
R1280 T2173 T2174 det the,interaction
R1281 T2174 T2172 pobj interaction,of
R1282 T2175 T2174 prep between,interaction
R1283 T2176 T2177 det the,RAD51
R1284 T2177 T2175 pobj RAD51,between
R1285 T2178 T2177 amod homologous,RAD51
R1286 T2179 T2177 compound recombinase,RAD51
R1287 T2180 T2177 cc and,RAD51
R1288 T2181 T2182 det a,peptide
R1289 T2182 T2177 conj peptide,RAD51
R1290 T2183 T2182 acl consisting,peptide
R1291 T2184 T2183 prep of,consisting
R1292 T2185 T2184 pobj one,of
R1293 T2186 T2185 prep of,one
R1294 T2187 T2188 det the,repeats
R1295 T2188 T2186 pobj repeats,of
R1296 T2189 T2188 nummod eight,repeats
R1297 T2190 T2188 compound BRC,repeats
R1298 T2191 T2188 prep from,repeats
R1299 T2192 T2193 amod human,BRCA2
R1300 T2193 T2191 pobj BRCA2,from
R1301 T2194 T2157 punct .,is
R1302 T2196 T2197 nsubjpass Expression,reported
R1303 T2198 T2196 prep of,Expression
R1304 T2199 T2200 det a,repeat
R1305 T2200 T2198 pobj repeat,of
R1306 T2201 T2200 amod single,repeat
R1307 T2202 T2200 compound BRC,repeat
R1308 T2203 T2200 punct (,repeat
R1309 T2204 T2200 appos BRC4,repeat
R1310 T2205 T2197 punct ),reported
R1311 T2206 T2197 aux had,reported
R1312 T2207 T2197 advmod previously,reported
R1313 T2208 T2197 auxpass been,reported
R1314 T2209 T2210 aux to,act
R1315 T2210 T2197 xcomp act,reported
R1316 T2211 T2210 prep as,act
R1317 T2212 T2213 det an,inhibitor
R1318 T2213 T2211 pobj inhibitor,as
R1319 T2214 T2213 prep of,inhibitor
R1320 T2215 T2216 compound DNA,repair
R1321 T2216 T2214 pobj repair,of
R1322 T2217 T2197 agent by,reported
R1323 T2218 T2217 pobj Chen,by
R1324 T2219 T2220 advmod et,al
R1325 T2220 T2218 advmod al,Chen
R1326 T2221 T2222 punct [,27
R1327 T2222 T2197 parataxis 27,reported
R1328 T2223 T2222 punct ],27
R1329 T2224 T2197 punct .,reported
R1330 T2226 T2227 det These,investigators
R1331 T2227 T2228 nsubj investigators,showed
R1332 T2229 T2230 mark that,enhances
R1333 T2230 T2228 ccomp enhances,showed
R1334 T2231 T2230 nsubj expression,enhances
R1335 T2232 T2231 prep of,expression
R1336 T2233 T2232 pobj constructs,of
R1337 T2234 T2233 acl containing,constructs
R1338 T2235 T2236 det the,repeat
R1339 T2236 T2234 dobj repeat,containing
R1340 T2237 T2236 compound BRC4,repeat
R1341 T2238 T2236 prep in,repeat
R1342 T2239 T2240 nmod MCF,cells
R1343 T2240 T2238 pobj cells,in
R1344 T2241 T2239 punct -,MCF
R1345 T2242 T2239 nummod 7,MCF
R1346 T2243 T2244 det the,radiosensitivity
R1347 T2244 T2230 dobj radiosensitivity,enhances
R1348 T2245 T2244 prep of,radiosensitivity
R1349 T2246 T2245 pobj cells,of
R1350 T2247 T2230 cc and,enhances
R1351 T2248 T2230 conj blocks,enhances
R1352 T2249 T2250 preconj both,delay
R1353 T2250 T2248 dobj delay,blocks
R1354 T2251 T2250 det the,delay
R1355 T2252 T2253 compound G2,M
R1356 T2253 T2250 compound M,delay
R1357 T2254 T2253 punct /,M
R1358 T2255 T2250 acl associated,delay
R1359 T2256 T2255 prep with,associated
R1360 T2257 T2256 pobj damage,with
R1361 T2258 T2250 cc and,delay
R1362 T2259 T2260 det the,ability
R1363 T2260 T2250 conj ability,delay
R1364 T2261 T2260 prep of,ability
R1365 T2262 T2263 det the,cells
R1366 T2263 T2261 pobj cells,of
R1367 T2264 T2263 amod transfected,cells
R1368 T2265 T2266 aux to,assembly
R1369 T2266 T2260 acl assembly,ability
R1370 T2267 T2268 amod subnuclear,foci
R1371 T2268 T2266 dobj foci,assembly
R1372 T2269 T2268 compound RAD51,foci
R1373 T2270 T2228 punct .,showed
R1374 T2272 T2273 prep In,provide
R1375 T2274 T2275 det the,paper
R1376 T2275 T2272 pobj paper,In
R1377 T2276 T2275 prep by,paper
R1378 T2277 T2276 pobj Davies,by
R1379 T2278 T2279 advmod et,al
R1380 T2279 T2277 advmod al,Davies
R1381 T2280 T2273 punct ", ",provide
R1382 T2281 T2273 nsubj data,provide
R1383 T2282 T2273 dobj evidence,provide
R1384 T2283 T2282 prep for,evidence
R1385 T2284 T2285 det a,mechanism
R1386 T2285 T2283 pobj mechanism,for
R1387 T2286 T2285 amod plausible,mechanism
R1388 T2287 T2285 prep of,mechanism
R1389 T2288 T2289 det the,effect
R1390 T2289 T2287 pobj effect,of
R1391 T2290 T2289 amod dominant,effect
R1392 T2291 T2289 amod negative,effect
R1393 T2292 T2289 acl associated,effect
R1394 T2293 T2292 prep with,associated
R1395 T2294 T2293 pobj expression,with
R1396 T2295 T2294 prep of,expression
R1397 T2296 T2297 det a,repeat
R1398 T2297 T2295 pobj repeat,of
R1399 T2298 T2297 amod single,repeat
R1400 T2299 T2297 compound BRC,repeat
R1401 T2300 T2273 punct .,provide
R1402 T2302 T2303 compound DNA,assays
R1403 T2303 T2305 nsubjpass assays,used
R1404 T2304 T2303 compound binding,assays
R1405 T2306 T2303 cc and,assays
R1406 T2307 T2308 compound electron,microscopy
R1407 T2308 T2309 compound microscopy,methods
R1408 T2309 T2303 conj methods,assays
R1409 T2310 T2305 auxpass were,used
R1410 T2311 T2312 aux to,show
R1411 T2312 T2305 advcl show,used
R1412 T2313 T2314 mark that,interferes
R1413 T2314 T2312 ccomp interferes,show
R1414 T2315 T2316 det a,peptide
R1415 T2316 T2314 nsubj peptide,interferes
R1416 T2317 T2316 compound BRC3,peptide
R1417 T2318 T2314 prep with,interferes
R1418 T2319 T2320 det the,ability
R1419 T2320 T2318 pobj ability,with
R1420 T2321 T2320 prep of,ability
R1421 T2322 T2321 pobj RAD51,of
R1422 T2323 T2324 aux to,assemble
R1423 T2324 T2320 acl assemble,ability
R1424 T2325 T2324 prep into,assemble
R1425 T2326 T2327 amod oligomeric,filaments
R1426 T2327 T2325 pobj filaments,into
R1427 T2328 T2324 prep on,assemble
R1428 T2329 T2328 pobj DNA,on
R1429 T2330 T2305 punct .,used
R1430 T2332 T2333 det A,peptide
R1431 T2333 T2335 nsubjpass peptide,reported
R1432 T2334 T2333 compound BRC4,peptide
R1433 T2336 T2335 auxpass was,reported
R1434 T2337 T2335 advmod also,reported
R1435 T2338 T2339 aux to,inhibit
R1436 T2339 T2335 xcomp inhibit,reported
R1437 T2340 T2341 det the,interaction
R1438 T2341 T2339 dobj interaction,inhibit
R1439 T2342 T2343 compound RAD51,DNA
R1440 T2343 T2341 compound DNA,interaction
R1441 T2344 T2343 punct –,DNA
R1442 T2345 T2335 punct .,reported
R1443 T2347 T2348 det These,experiments
R1444 T2348 T2349 nsubj experiments,raise
R1445 T2350 T2351 det the,possibility
R1446 T2351 T2349 dobj possibility,raise
R1447 T2352 T2353 mark that,act
R1448 T2353 T2351 acl act,possibility
R1449 T2354 T2355 det the,protein
R1450 T2355 T2353 nsubj protein,act
R1451 T2356 T2357 amod full,length
R1452 T2357 T2355 compound length,protein
R1453 T2358 T2357 punct -,length
R1454 T2359 T2355 compound BRCA2,protein
R1455 T2360 T2353 aux could,act
R1456 T2361 T2353 prep as,act
R1457 T2362 T2363 det a,regulator
R1458 T2363 T2361 pobj regulator,as
R1459 T2364 T2363 amod negative,regulator
R1460 T2365 T2363 prep of,regulator
R1461 T2366 T2367 amod inappropriate,interaction
R1462 T2367 T2365 pobj interaction,of
R1463 T2368 T2369 compound RAD51,DNA
R1464 T2369 T2367 compound DNA,interaction
R1465 T2370 T2369 punct –,DNA
R1466 T2371 T2349 punct .,raise
R1467 T2373 T2374 det The,experiment
R1468 T2374 T2376 nsubj experiment,is
R1469 T2375 T2374 amod final,experiment
R1470 T2377 T2374 acl reported,experiment
R1471 T2378 T2377 agent by,reported
R1472 T2379 T2378 pobj Davies,by
R1473 T2380 T2381 advmod et,al
R1474 T2381 T2379 advmod al,Davies
R1475 T2382 T2383 punct [,26
R1476 T2383 T2379 parataxis 26,Davies
R1477 T2384 T2383 punct ],26
R1478 T2385 T2386 advmod particularly,important
R1479 T2386 T2376 acomp important,is
R1480 T2387 T2386 prep with,important
R1481 T2388 T2387 pobj respect,with
R1482 T2389 T2388 prep to,respect
R1483 T2390 T2389 pcomp understanding,to
R1484 T2391 T2392 det the,s
R1485 T2392 T2390 dobj s,understanding
R1486 T2393 T2392 nmod mechanism,s
R1487 T2394 T2392 punct (,s
R1488 T2395 T2392 punct ),s
R1489 T2396 T2397 prep through,promotes
R1490 T2397 T2392 relcl promotes,s
R1491 T2398 T2396 pobj which,through
R1492 T2399 T2397 nsubj BRCA2,promotes
R1493 T2400 T2401 npadvmod RAD51,dependent
R1494 T2401 T2403 amod dependent,recombination
R1495 T2402 T2401 punct -,dependent
R1496 T2403 T2397 dobj recombination,promotes
R1497 T2404 T2376 punct .,is
R1498 T2406 T2407 det The,localization
R1499 T2407 T2408 nsubjpass localization,examined
R1500 T2409 T2407 prep of,localization
R1501 T2410 T2409 pobj RAD51,of
R1502 T2411 T2408 auxpass was,examined
R1503 T2412 T2408 prep in,examined
R1504 T2413 T2414 nmod CAPAN,cells
R1505 T2414 T2412 pobj cells,in
R1506 T2415 T2413 punct -,CAPAN
R1507 T2416 T2413 nummod 1,CAPAN
R1508 T2417 T2414 punct ", ",cells
R1509 T2418 T2419 det the,line
R1510 T2419 T2414 appos line,cells
R1511 T2420 T2419 amod same,line
R1512 T2421 T2422 amod human,tumor
R1513 T2422 T2419 compound tumor,line
R1514 T2423 T2419 acl examined,line
R1515 T2424 T2423 agent by,examined
R1516 T2425 T2424 pobj Moynahan,by
R1517 T2426 T2427 advmod et,al
R1518 T2427 T2425 advmod al,Moynahan
R1519 T2428 T2429 punct [,24
R1520 T2429 T2425 parataxis 24,Moynahan
R1521 T2430 T2429 punct ],24
R1522 T2431 T2408 punct .,examined
R1523 T2433 T2434 nsubjpass It,shown
R1524 T2435 T2434 aux had,shown
R1525 T2436 T2434 auxpass been,shown
R1526 T2437 T2434 advmod previously,shown
R1527 T2438 T2439 mark that,mislocalized
R1528 T2439 T2434 ccomp mislocalized,shown
R1529 T2440 T2441 det the,amount
R1530 T2441 T2439 nsubjpass amount,mislocalized
R1531 T2442 T2441 amod small,amount
R1532 T2443 T2441 prep of,amount
R1533 T2444 T2445 amod truncated,protein
R1534 T2445 T2443 pobj protein,of
R1535 T2446 T2445 compound BRCA2,protein
R1536 T2447 T2441 acl expressed,amount
R1537 T2448 T2447 prep by,expressed
R1538 T2449 T2450 nmod CAPAN,cells
R1539 T2450 T2448 pobj cells,by
R1540 T2451 T2449 punct -,CAPAN
R1541 T2452 T2449 nummod 1,CAPAN
R1542 T2453 T2439 auxpass was,mislocalized
R1543 T2454 T2439 prep to,mislocalized
R1544 T2455 T2456 det the,cytoplasm
R1545 T2456 T2454 pobj cytoplasm,to
R1546 T2457 T2439 punct ", ",mislocalized
R1547 T2458 T2439 advcl consistent,mislocalized
R1548 T2459 T2458 prep with,consistent
R1549 T2460 T2461 det the,fact
R1550 T2461 T2459 pobj fact,with
R1551 T2462 T2463 mark that,lacks
R1552 T2463 T2461 acl lacks,fact
R1553 T2464 T2465 det the,protein
R1554 T2465 T2463 nsubj protein,lacks
R1555 T2466 T2465 compound truncation,protein
R1556 T2467 T2468 det a,signal
R1557 T2468 T2463 dobj signal,lacks
R1558 T2469 T2468 amod nuclear,signal
R1559 T2470 T2468 compound localization,signal
R1560 T2471 T2472 punct [,28
R1561 T2472 T2434 parataxis 28,shown
R1562 T2473 T2472 punct ],28
R1563 T2474 T2434 punct .,shown
R1564 T2476 T2477 nsubj Davies,showed
R1565 T2478 T2479 advmod et,al
R1566 T2479 T2476 advmod al,Davies
R1567 T2480 T2481 nmod CAPAN,cells
R1568 T2481 T2484 nsubj cells,be
R1569 T2482 T2480 punct -,CAPAN
R1570 T2483 T2480 nummod 1,CAPAN
R1571 T2484 T2477 ccomp be,showed
R1572 T2485 T2484 aux to,be
R1573 T2486 T2484 acomp defective,be
R1574 T2487 T2484 prep in,be
R1575 T2488 T2489 amod nuclear,localization
R1576 T2489 T2487 pobj localization,in
R1577 T2490 T2489 prep of,localization
R1578 T2491 T2490 pobj RAD51,of
R1579 T2492 T2477 punct ", ",showed
R1580 T2493 T2477 advcl raising,showed
R1581 T2494 T2495 det the,possibility
R1582 T2495 T2493 dobj possibility,raising
R1583 T2496 T2497 mark that,carried
R1584 T2497 T2495 acl carried,possibility
R1585 T2498 T2497 nsubjpass RAD51,carried
R1586 T2499 T2497 auxpass is,carried
R1587 T2500 T2497 advmod normally,carried
R1588 T2501 T2497 prep to,carried
R1589 T2502 T2503 det the,nucleus
R1590 T2503 T2501 pobj nucleus,to
R1591 T2504 T2497 prep by,carried
R1592 T2505 T2504 pcomp binding,by
R1593 T2506 T2505 dobj BRCA2,binding
R1594 T2507 T2477 punct .,showed
R1595 T2509 T2510 nsubj It,is
R1596 T2511 T2510 acomp possible,is
R1597 T2512 T2513 mark that,be
R1598 T2513 T2510 ccomp be,is
R1599 T2514 T2513 nsubj RAD51,be
R1600 T2515 T2513 aux may,be
R1601 T2516 T2513 advmod alternatively,be
R1602 T2517 T2513 acomp capable,be
R1603 T2518 T2517 prep of,capable
R1604 T2519 T2520 npadvmod BRCA2,independent
R1605 T2520 T2522 amod independent,transport
R1606 T2521 T2520 punct -,independent
R1607 T2522 T2518 pobj transport,of
R1608 T2523 T2510 punct ", ",is
R1609 T2524 T2510 cc but,is
R1610 T2525 T2526 nsubj mislocalization,sequester
R1611 T2526 T2510 conj sequester,is
R1612 T2527 T2525 prep of,mislocalization
R1613 T2528 T2527 pobj BRCA2,of
R1614 T2529 T2525 prep to,mislocalization
R1615 T2530 T2531 det the,cytoplasm
R1616 T2531 T2529 pobj cytoplasm,to
R1617 T2532 T2526 aux may,sequester
R1618 T2533 T2526 dobj RAD51,sequester
R1619 T2534 T2526 cc and,sequester
R1620 T2535 T2526 conj block,sequester
R1621 T2536 T2537 poss its,mode
R1622 T2537 T2535 dobj mode,block
R1623 T2538 T2537 amod normal,mode
R1624 T2539 T2537 prep of,mode
R1625 T2540 T2539 pobj transport,of
R1626 T2541 T2526 punct .,sequester
R1627 T2543 T2544 nsubj Support,requires
R1628 T2545 T2543 prep for,Support
R1629 T2546 T2547 det the,hypothesis
R1630 T2547 T2545 pobj hypothesis,for
R1631 T2548 T2549 mark that,is
R1632 T2549 T2547 acl is,hypothesis
R1633 T2550 T2551 det the,mislocalization
R1634 T2551 T2549 nsubj mislocalization,is
R1635 T2552 T2551 amod observed,mislocalization
R1636 T2553 T2551 prep of,mislocalization
R1637 T2554 T2553 pobj RAD51,of
R1638 T2555 T2556 det an,consequence
R1639 T2556 T2549 attr consequence,is
R1640 T2557 T2556 amod indirect,consequence
R1641 T2558 T2556 prep of,consequence
R1642 T2559 T2560 det the,defect
R1643 T2560 T2558 pobj defect,of
R1644 T2561 T2560 compound BRCA2,defect
R1645 T2562 T2544 dobj reintroduction,requires
R1646 T2563 T2562 prep of,reintroduction
R1647 T2564 T2565 amod functional,BRCA2
R1648 T2565 T2563 pobj BRCA2,of
R1649 T2566 T2562 prep into,reintroduction
R1650 T2567 T2566 pobj CAPAN,into
R1651 T2568 T2567 punct -,CAPAN
R1652 T2569 T2567 nummod 1,CAPAN
R1653 T2570 T2544 punct .,requires
R1654 T2572 T2573 prep Given,seems
R1655 T2574 T2575 det the,nature
R1656 T2575 T2572 pobj nature,Given
R1657 T2576 T2575 amod elaborate,nature
R1658 T2577 T2575 prep of,nature
R1659 T2578 T2579 det the,interaction
R1660 T2579 T2577 pobj interaction,of
R1661 T2580 T2581 compound RAD51,BRCA2
R1662 T2581 T2579 compound BRCA2,interaction
R1663 T2582 T2581 punct –,BRCA2
R1664 T2583 T2573 punct ", ",seems
R1665 T2584 T2573 nsubj it,seems
R1666 T2585 T2586 advmod somewhat,unlikely
R1667 T2586 T2573 oprd unlikely,seems
R1668 T2587 T2588 mark that,is
R1669 T2588 T2573 ccomp is,seems
R1670 T2589 T2590 det the,role
R1671 T2590 T2588 nsubj role,is
R1672 T2591 T2590 amod only,role
R1673 T2592 T2590 prep of,role
R1674 T2593 T2592 pobj BRCA2,of
R1675 T2594 T2590 prep in,role
R1676 T2595 T2594 pcomp promoting,in
R1677 T2596 T2595 dobj recombination,promoting
R1678 T2597 T2598 aux to,serve
R1679 T2598 T2588 xcomp serve,is
R1680 T2599 T2598 prep as,serve
R1681 T2600 T2601 det a,transporter
R1682 T2601 T2599 pobj transporter,as
R1683 T2602 T2601 amod specific,transporter
R1684 T2603 T2601 amod nuclear,transporter
R1685 T2604 T2601 prep of,transporter
R1686 T2605 T2604 pobj RAD51,of
R1687 T2606 T2573 punct .,seems
R1688 T2608 T2609 det An,alternative
R1689 T2609 T2611 nsubj alternative,is
R1690 T2610 T2609 amod attractive,alternative
R1691 T2612 T2609 punct ", ",alternative
R1692 T2613 T2609 acl favored,alternative
R1693 T2614 T2613 agent by,favored
R1694 T2615 T2614 pobj Davies,by
R1695 T2616 T2617 advmod et,al
R1696 T2617 T2615 advmod al,Davies
R1697 T2618 T2619 punct [,26
R1698 T2619 T2615 parataxis 26,Davies
R1699 T2620 T2619 punct ],26
R1700 T2621 T2611 punct ", ",is
R1701 T2622 T2623 mark that,required
R1702 T2623 T2611 ccomp required,is
R1703 T2624 T2625 det the,association
R1704 T2625 T2623 nsubjpass association,required
R1705 T2626 T2625 prep of,association
R1706 T2627 T2628 compound RAD51,BRCA2
R1707 T2628 T2626 pobj BRCA2,of
R1708 T2629 T2628 punct –,BRCA2
R1709 T2630 T2623 auxpass is,required
R1710 T2631 T2632 aux to,maintain
R1711 T2632 T2623 advcl maintain,required
R1712 T2633 T2632 dobj RAD51,maintain
R1713 T2634 T2632 prep in,maintain
R1714 T2635 T2636 det a,form
R1715 T2636 T2634 pobj form,in
R1716 T2637 T2638 dep that,is
R1717 T2638 T2636 relcl is,form
R1718 T2639 T2638 prep in,is
R1719 T2640 T2641 det a,state
R1720 T2641 T2639 pobj state,in
R1721 T2642 T2641 prep of,state
R1722 T2643 T2642 punct ',of
R1723 T2644 T2642 pobj readiness,of
R1724 T2645 T2611 punct ',is
R1725 T2646 T2647 punct (,2a
R1726 T2647 T2611 parataxis 2a,is
R1727 T2648 T2647 compound Fig.,2a
R1728 T2649 T2647 punct ),2a
R1729 T2650 T2611 punct .,is
R1730 T2652 T2653 prep In,exist
R1731 T2654 T2655 det the,absence
R1732 T2655 T2652 pobj absence,In
R1733 T2656 T2655 prep of,absence
R1734 T2657 T2658 det this,interaction
R1735 T2658 T2656 pobj interaction,of
R1736 T2659 T2658 prep with,interaction
R1737 T2660 T2659 pobj BRCA2,with
R1738 T2661 T2653 punct ", ",exist
R1739 T2662 T2653 nsubj RAD51,exist
R1740 T2663 T2653 aux might,exist
R1741 T2664 T2653 prep in,exist
R1742 T2665 T2666 det a,form
R1743 T2666 T2664 pobj form,in
R1744 T2667 T2668 dep that,is
R1745 T2668 T2666 relcl is,form
R1746 T2669 T2668 neg not,is
R1747 T2670 T2668 acomp capable,is
R1748 T2671 T2670 prep of,capable
R1749 T2672 T2673 auxpass being,recruited
R1750 T2673 T2671 pcomp recruited,of
R1751 T2674 T2673 prep into,recruited
R1752 T2675 T2676 det a,complex
R1753 T2676 T2674 pobj complex,into
R1754 T2677 T2676 amod functional,complex
R1755 T2678 T2676 compound repair,complex
R1756 T2679 T2680 advmod when,occurs
R1757 T2680 T2673 advcl occurs,recruited
R1758 T2681 T2680 nsubj damage,occurs
R1759 T2682 T2653 punct .,exist
R1760 T2684 T2685 prep For,indicated
R1761 T2686 T2684 pobj example,For
R1762 T2687 T2685 punct ", ",indicated
R1763 T2688 T2689 amod previous,studies
R1764 T2689 T2685 nsubj studies,indicated
R1765 T2690 T2691 mark that,shows
R1766 T2691 T2685 ccomp shows,indicated
R1767 T2692 T2691 nsubj RAD51,shows
R1768 T2693 T2694 amod little,preference
R1769 T2694 T2691 dobj preference,shows
R1770 T2695 T2694 compound binding,preference
R1771 T2696 T2694 prep for,preference
R1772 T2697 T2698 amod single,strand
R1773 T2698 T2700 compound strand,DNA
R1774 T2699 T2698 punct -,strand
R1775 T2700 T2696 pobj DNA,for
R1776 T2701 T2700 punct (,DNA
R1777 T2702 T2700 appos ssDNA,DNA
R1778 T2703 T2694 punct ),preference
R1779 T2704 T2694 prep over,preference
R1780 T2705 T2706 amod double,strand
R1781 T2706 T2708 compound strand,DNA
R1782 T2707 T2706 punct -,strand
R1783 T2708 T2704 pobj DNA,over
R1784 T2709 T2708 punct (,DNA
R1785 T2710 T2708 appos dsDNA,DNA
R1786 T2711 T2685 punct ),indicated
R1787 T2712 T2713 punct [,29
R1788 T2713 T2685 parataxis 29,indicated
R1789 T2714 T2713 nummod 3,29
R1790 T2715 T2713 punct ",",29
R1791 T2716 T2713 punct ],29
R1792 T2717 T2685 punct .,indicated
R1793 T2719 T2720 det This,result
R1794 T2720 T2721 nsubj result,mean
R1795 T2722 T2721 aux could,mean
R1796 T2723 T2724 mark that,required
R1797 T2724 T2721 ccomp required,mean
R1798 T2725 T2726 amod regulatory,factors
R1799 T2726 T2724 nsubjpass factors,required
R1800 T2727 T2726 punct ", ",factors
R1801 T2728 T2729 amod such,as
R1802 T2729 T2726 prep as,factors
R1803 T2730 T2729 pobj BRCA2,as
R1804 T2731 T2724 punct ", ",required
R1805 T2732 T2724 auxpass are,required
R1806 T2733 T2734 aux to,suppress
R1807 T2734 T2724 advcl suppress,required
R1808 T2735 T2736 compound RAD51,dsDNA
R1809 T2736 T2738 compound dsDNA,interactions
R1810 T2737 T2736 punct –,dsDNA
R1811 T2738 T2734 dobj interactions,suppress
R1812 T2740 T2724 advcl prevent,required
R1813 T2741 T2740 dobj sequestration,prevent
R1814 T2742 T2741 prep of,sequestration
R1815 T2743 T2742 pobj RAD51,of
R1816 T2744 T2741 prep in,sequestration
R1817 T2745 T2746 det a,form
R1818 T2746 T2744 pobj form,in
R1819 T2747 T2746 amod nonfunctional,form
R1820 T2748 T2721 punct .,mean
R1821 T2750 T2751 advmod Alternatively,in
R1822 T2751 T2754 prep in,be
R1823 T2752 T2751 punct ", ",in
R1824 T2739 T2740 aux to,prevent
R1825 T2755 T2751 pobj addition,in
R1826 T2756 T2754 punct ", ",be
R1827 T2753 T2751 cc or,in
R1828 T2758 T2757 prep of,suppression
R1829 T2759 T2760 compound RAD51,ssDNA
R1830 T2757 T2754 nsubj suppression,be
R1831 T2761 T2760 punct –,ssDNA
R1832 T2762 T2758 pobj interaction,of
R1833 T2760 T2762 compound ssDNA,interaction
R1834 T2764 T2754 acomp important,be
R1835 T2765 T2754 prep for,be
R1836 T2763 T2754 aux might,be
R1837 T2767 T2766 dobj RAD51,preventing
R1838 T2768 T2766 prep from,preventing
R1839 T2766 T2765 pcomp preventing,for
R1840 T2770 T2769 prep to,binding
R1841 T2771 T2772 det the,regions
R1842 T2769 T2768 pcomp binding,from
R1843 T2773 T2772 prep of,regions
R1844 T2774 T2773 pobj ssDNA,of
R1845 T2772 T2770 pobj regions,to
R1846 T2776 T2772 relcl form,regions
R1847 T2777 T2776 prep during,form
R1848 T2775 T2776 dep that,form
R1849 T2779 T2777 pobj replication,during
R1850 T2780 T2779 compound DNA,replication
R1851 T2781 T2754 punct .,be
R1852 T2778 T2779 amod normal,replication
R1853 T2783 T2784 nsubj BRCA2,promote
R1854 T2785 T2784 aux may,promote
R1855 T2786 T2784 advmod thus,promote
R1856 T2788 T2784 dobj assembly,promote
R1857 T2789 T2788 prep into,assembly
R1858 T2787 T2788 compound RAD51,assembly
R1859 T2791 T2789 pobj complexes,into
R1860 T2792 T2791 compound repair,complexes
R1861 T2790 T2791 amod recombinational,complexes
R1862 T2794 T2795 det a,mechanism
R1863 T2795 T2793 pobj mechanism,via
R1864 T2796 T2795 amod negative,mechanism
R1865 T2793 T2784 prep via,promote
R1866 T2798 T2793 punct (,via
R1867 T2799 T2800 advmod i.e.,by
R1868 T2797 T2795 amod regulatory,mechanism
R1869 T2801 T2800 pcomp blocking,by
R1870 T2802 T2803 compound RAD51–BRCA2,interaction
R1871 T2800 T2793 prep by,via
R1872 T2804 T2805 mark until,occurred
R1873 T2805 T2801 advcl occurred,blocking
R1874 T2803 T2801 dobj interaction,blocking
R1875 T2807 T2805 aux has,occurred
R1876 T2808 T2805 cc and,occurred
R1877 T2806 T2805 nsubj damage,occurred
R1878 T2810 T2805 conj are,occurred
R1879 T2811 T2809 acl required,factors
R1880 T2809 T2810 nsubj factors,are
R1881 T2813 T2812 punct ',for
R1882 T2812 T2811 prep for,required
R1883 T2815 T2812 pobj assembly,for
R1884 T2814 T2815 amod productive,assembly
R1885 T2817 T2815 prep of,assembly
R1886 T2816 T2815 punct ',assembly
R1887 T2819 T2815 prep at,assembly
R1888 T2818 T2817 pobj RAD51,of
R1889 T2821 T2819 pobj sites,at
R1890 T2822 T2810 prep in,are
R1891 T2820 T2821 amod damaged,sites
R1892 T2824 T2784 punct ),promote
R1893 T2825 T2784 punct .,promote
R1894 T2823 T2822 pobj place,in
R1895 T2827 T2828 mark As,mentioned
R1896 T2828 T2829 advcl mentioned,reflect
R1897 T2830 T2828 advmod previously,mentioned
R1898 T2831 T2829 punct ", ",reflect
R1899 T2833 T2829 nsubj ability,reflect
R1900 T2832 T2833 det the,ability
R1901 T2835 T2836 det a,peptide
R1902 T2836 T2834 pobj peptide,of
R1903 T2834 T2833 prep of,ability
R1904 T2838 T2839 aux to,repress
R1905 T2839 T2833 acl repress,ability
R1906 T2837 T2836 compound BRC,peptide
R1907 T2841 T2839 dobj interaction,repress
R1908 T2842 T2841 compound RAD51,interaction
R1909 T2840 T2841 det the,interaction
R1910 T2844 T2841 compound BRCA2,interaction
R1911 T2843 T2841 punct –,interaction
R1912 T2846 T2847 det a,role
R1913 T2847 T2829 dobj role,reflect
R1914 T2845 T2829 aux could,reflect
R1915 T2849 T2850 amod full,length
R1916 T2850 T2852 compound length,protein
R1917 T2851 T2850 punct -,length
R1918 T2848 T2847 prep of,role
R1919 T2853 T2847 prep in,role
R1920 T2852 T2848 pobj protein,of
R1921 T2855 T2856 amod unwanted,interactions
R1922 T2854 T2853 pcomp suppressing,in
R1923 T2857 T2858 compound RAD51,BRCA2
R1924 T2858 T2856 compound BRCA2,interactions
R1925 T2856 T2854 dobj interactions,suppressing
R1926 T2860 T2829 punct .,reflect
R1927 T2862 T2863 advmod Yet,role
R1928 T2859 T2858 punct –,BRCA2
R1929 T2864 T2863 det another,role
R1930 T2867 T2863 prep for,role
R1931 T2863 T2866 nsubj role,is
R1932 T2865 T2863 amod potential,role
R1933 T2869 T2863 prep in,role
R1934 T2870 T2869 pcomp promoting,in
R1935 T2868 T2867 pobj BRCA2,for
R1936 T2872 T2874 amod dependent,repair
R1937 T2873 T2872 punct -,dependent
R1938 T2871 T2872 npadvmod RAD51,dependent
R1939 T2875 T2874 amod recombinational,repair
R1940 T2876 T2877 det a,role
R1941 T2874 T2870 dobj repair,promoting
R1942 T2878 T2877 amod positive,role
R1943 T2877 T2866 attr role,is
R1944 T2880 T2879 pobj assembly,in
R1945 T2881 T2880 prep of,assembly
R1946 T2879 T2877 prep in,role
R1947 T2883 T2880 prep at,assembly
R1948 T2884 T2885 amod damaged,sites
R1949 T2882 T2881 pobj RAD51,of
R1950 T2886 T2887 punct (,2b
R1951 T2885 T2883 pobj sites,at
R1952 T2888 T2887 compound Fig.,2b
R1953 T2889 T2887 punct ),2b
R1954 T2887 T2866 parataxis 2b,is
R1955 T2892 T2893 amod Several,proteins
R1956 T2890 T2866 punct .,is
R1957 T2895 T2896 dep that,interact
R1958 T2893 T2894 nsubjpass proteins,thought
R1959 T2897 T2896 prep with,interact
R1960 T2898 T2897 pobj RAD51,with
R1961 T2896 T2893 relcl interact,proteins
R1962 T2900 T2901 aux to,mediate
R1963 T2901 T2894 xcomp mediate,thought
R1964 T2899 T2894 auxpass are,thought
R1965 T2903 T2901 punct ',mediate
R1966 T2904 T2901 dobj assembly,mediate
R1967 T2902 T2901 punct ',mediate
R1968 T2906 T2905 pobj RAD51,of
R1969 T2907 T2901 prep at,mediate
R1970 T2905 T2904 prep of,assembly
R1971 T2909 T2908 prep of,sites
R1972 T2910 T2911 compound DNA,damage
R1973 T2908 T2907 pobj sites,at
R1974 T2912 T2913 punct (,reviewed
R1975 T2913 T2894 parataxis reviewed,thought
R1976 T2911 T2909 pobj damage,of
R1977 T2914 T2913 prep in,reviewed
R1978 T2916 T2914 pobj 30,in
R1979 T2917 T2913 punct ],reviewed
R1980 T2915 T2914 punct [,in
R1981 T2919 T2894 punct .,thought
R1982 T2918 T2913 punct ),reviewed
R1983 T2921 T2922 compound RAD51,paralogs
R1984 T2924 T2922 prep in,paralogs
R1985 T2922 T2923 nsubj paralogs,function
R1986 T2926 T2922 punct ", ",paralogs
R1987 T2925 T2924 amod particular,in
R1988 T2928 T2927 pobj XRCC2,including
R1989 T2929 T2928 punct ", ",XRCC2
R1990 T2927 T2922 prep including,paralogs
R1991 T2931 T2930 punct ", ",XRCC3
R1992 T2932 T2930 conj RAD51B,XRCC3
R1993 T2930 T2928 conj XRCC3,XRCC2
R1994 T2934 T2932 conj RAD51C,RAD51B
R1995 T2935 T2934 cc and,RAD51C
R1996 T2933 T2932 punct ", ",RAD51B
R1997 T2937 T2923 punct ", ",function
R1998 T2938 T2923 aux may,function
R1999 T2936 T2934 conj RAD51D,RAD51C
R2000 T2940 T2941 det a,complex
R2001 T2941 T2939 pobj complex,as
R2002 T2939 T2923 prep as,function
R2003 T2943 T2941 relcl promotes,complex
R2004 T2944 T2943 advmod actively,promotes
R2005 T2942 T2943 dep that,promotes
R2006 T2946 T2943 dobj assembly,promotes
R2007 T2947 T2948 punct [,31
R2008 T2945 T2946 compound RAD51,assembly
R2009 T2949 T2948 punct ],31
R2010 T2950 T2923 punct .,function
R2011 T2948 T2923 parataxis 31,function
R2012 T2952 T2953 nsubj BRCA2,cooperate
R2013 T2954 T2953 aux could,cooperate
R2014 T2956 T2957 compound RAD51,paralogs
R2015 T2957 T2955 pobj paralogs,with
R2016 T2955 T2953 prep with,cooperate
R2017 T2959 T2958 pcomp promoting,in
R2018 T2960 T2961 compound RAD51,assembly
R2019 T2958 T2953 prep in,cooperate
R2020 T2962 T2953 punct ", ",cooperate
R2021 T2963 T2953 cc or,cooperate
R2022 T2961 T2959 dobj assembly,promoting
R2023 T2965 T2953 conj provide,cooperate
R2024 T2966 T2967 det an,pathway
R2025 T2964 T2965 advmod even,provide
R2026 T2968 T2967 amod alternative,pathway
R2027 T2969 T2967 compound assembly,pathway
R2028 T2967 T2965 dobj pathway,provide
R2029 T2972 T2973 det The,observation
R2030 T2970 T2953 punct .,cooperate
R2031 T2975 T2976 mark that,localizes
R2032 T2976 T2973 acl localizes,observation
R2033 T2973 T2974 nsubj observation,seems
R2034 T2978 T2976 prep to,localizes
R2035 T2979 T2980 npadvmod damage,induced
R2036 T2977 T2976 nsubj BRCA2,localizes
R2037 T2981 T2980 punct -,induced
R2038 T2980 T2982 amod induced,foci
R2039 T2983 T2982 amod subnuclear,foci
R2040 T2982 T2978 pobj foci,to
R2041 T2985 T2984 pobj RAD51,with
R2042 T2986 T2974 oprd consistent,seems
R2043 T2984 T2976 prep with,localizes
R2044 T2988 T2989 det the,possibility
R2045 T2987 T2986 prep with,consistent
R2046 T2990 T2991 mark that,plays
R2047 T2989 T2987 pobj possibility,with
R2048 T2992 T2991 nsubj BRCA2,plays
R2049 T2991 T2989 acl plays,possibility
R2050 T2994 T2991 dobj role,plays
R2051 T2995 T2994 amod positive,role
R2052 T2993 T2994 det a,role
R2053 T2997 T2996 pobj assembly,in
R2054 T2996 T2991 prep in,plays
R2055 T2999 T3000 det the,complex
R2056 T3000 T2998 pobj complex,of
R2057 T2998 T2997 prep of,assembly
R2058 T3002 T3003 punct [,11
R2059 T3001 T3000 compound recombination,complex
R2060 T3004 T3003 punct ],11
R2061 T3003 T2974 parataxis 11,seems
R2062 T3007 T3008 advmod Finally,is
R2063 T3005 T2974 punct .,seems
R2064 T3009 T3008 punct ", ",is
R2065 T3011 T3008 acomp important,is
R2066 T3012 T3013 aux to,note
R2067 T3010 T3008 nsubj it,is
R2068 T3014 T3015 mark that,are
R2069 T3015 T3013 ccomp are,note
R2070 T3013 T3008 xcomp note,is
R2071 T3017 T3015 nsubj models,are
R2072 T3018 T3017 nummod two,models
R2073 T3016 T3017 det the,models
R2074 T3020 T3021 det the,function
R2075 T3021 T3019 pobj function,for
R2076 T3022 T3021 prep of,function
R2077 T3019 T3017 prep for,models
R2078 T3024 T3022 pobj interaction,of
R2079 T3025 T3026 compound BRCA2,RAD51
R2080 T3023 T3024 det the,interaction
R2081 T3026 T3024 compound RAD51,interaction
R2082 T3028 T3017 acl shown,models
R2083 T3029 T3028 prep in,shown
R2084 T3027 T3026 punct –,RAD51
R2085 T3031 T3030 nummod 2,Figure
R2086 T3030 T3029 pobj Figure,in
R2087 T3033 T3034 advmod mutually,exclusive
R2088 T3034 T3015 acomp exclusive,are
R2089 T3032 T3015 neg not,are
R2090 T3039 T3038 aux could,prevent
R2091 T3035 T3008 punct .,is
R2092 T3041 T3038 dobj assembly,prevent
R2093 T3037 T3038 nsubj BRCA2,prevent
R2094 T3042 T3041 compound RAD51,assembly
R2095 T3040 T3041 amod inappropriate,assembly
R2096 T3044 T3045 det the,absence
R2097 T3043 T3038 prep in,prevent
R2098 T3046 T3045 prep of,absence
R2099 T3045 T3043 pobj absence,in
R2100 T3048 T3038 cc and,prevent
R2101 T3049 T3038 conj promote,prevent
R2102 T3047 T3046 pobj damage,of
R2103 T3051 T3049 dobj assembly,promote
R2104 T3052 T3051 compound RAD51,assembly
R2105 T3050 T3051 amod functional,assembly
R2106 T3054 T3055 compound DNA,lesions
R2107 T3055 T3053 pobj lesions,at
R2108 T3053 T3051 prep at,assembly
R2109 T3057 T3058 det the,response
R2110 T3056 T3049 prep during,promote
R2111 T3059 T3058 compound damage,response
R2112 T3060 T3038 punct .,prevent
R2113 T3058 T3056 pobj response,during
R2114 T3062 T3063 amod Further,experiments
R2115 T3065 T3064 auxpass are,required
R2116 T3066 T3064 advmod clearly,required
R2117 T3063 T3064 nsubjpass experiments,required
R2118 T3068 T3064 advcl clarify,required
R2119 T3069 T3070 det the,interactions
R2120 T3067 T3068 aux to,clarify
R2121 T3071 T3070 amod functional,interactions
R2122 T3070 T3068 dobj interactions,clarify
R2123 T3073 T3072 pobj BRCA2,between
R2124 T3074 T3073 cc and,BRCA2
R2125 T3072 T3070 prep between,interactions
R2126 T3076 T3068 prep during,clarify
R2127 T3077 T3078 amod recombinational,repair
R2128 T3075 T3073 conj RAD51,BRCA2
R2129 T3079 T3064 punct .,required
R2130 T3078 T3076 pobj repair,during
R2131 T3083 T3082 aux should,be
R2132 T3084 T3085 advmod relatively,straightforward
R2133 T3085 T3082 acomp straightforward,be
R2134 T3081 T3082 nsubj It,be
R2135 T3087 T3082 xcomp determine,be
R2136 T3089 T3087 ccomp limited,determine
R2137 T3086 T3087 aux to,determine
R2138 T3090 T3091 det the,role
R2139 T3088 T3089 mark whether,limited
R2140 T3092 T3091 prep of,role
R2141 T3091 T3089 nsubjpass role,limited
R2142 T3094 T3089 auxpass is,limited
R2143 T3095 T3089 prep to,limited
R2144 T3093 T3092 pobj BRCA2,of
R2145 T3097 T3096 dobj RAD51,transporting
R2146 T3096 T3095 pcomp transporting,to
R2147 T3099 T3100 det the,nucleus
R2148 T3100 T3098 pobj nucleus,to
R2149 T3098 T3096 prep to,transporting
R2150 T3103 T3104 mark If,were
R2151 T3101 T3082 punct .,be
R2152 T3105 T3104 nsubj this,were
R2153 T3108 T3104 attr case,were
R2154 T3104 T3106 advcl were,suppress
R2155 T3109 T3106 punct ", ",suppress
R2156 T3107 T3108 det the,case
R2157 T3111 T3110 prep of,addition
R2158 T3112 T3113 det a,signal
R2159 T3113 T3111 pobj signal,of
R2160 T3110 T3106 nsubj addition,suppress
R2161 T3115 T3113 compound localization,signal
R2162 T3116 T3110 prep to,addition
R2163 T3114 T3113 amod nuclear,signal
R2164 T3118 T3106 aux should,suppress
R2165 T3119 T3120 det the,phenotypes
R2166 T3117 T3116 pobj RAD51,to
R2167 T3121 T3122 npadvmod RAD51,mediated
R2168 T3122 T3120 amod mediated,phenotypes
R2169 T3120 T3106 dobj phenotypes,suppress
R2170 T3124 T3120 acl associated,phenotypes
R2171 T3125 T3124 prep with,associated
R2172 T3123 T3122 punct -,mediated
R2173 T3127 T3125 pobj mutation,with
R2174 T3128 T3127 compound BRCA2,mutation
R2175 T3126 T3127 det a,mutation
R2176 T3133 T3132 punct ", ",needed
R2177 T3129 T3106 punct .,suppress
R2178 T3131 T3132 advmod However,needed
R2179 T3135 T3132 nsubjpass experiments,needed
R2180 T3136 T3132 auxpass are,needed
R2181 T3134 T3135 amod biochemical,experiments
R2182 T3138 T3132 advcl determine,needed
R2183 T3137 T3138 aux to,determine
R2184 T3140 T3138 ccomp blocks,determine
R2185 T3141 T3142 amod full,length
R2186 T3139 T3140 mark whether,blocks
R2187 T3143 T3142 punct -,length
R2188 T3144 T3140 nsubj BRCA2,blocks
R2189 T3142 T3144 compound length,BRCA2
R2192 T3145 T3140 cc or,blocks
R135 T227 T228 nsubj BRCA2,was
R136 T229 T230 det the,gene
R137 T230 T228 attr gene,was
R138 T231 T230 amod second,gene
R139 T232 T233 compound breast,cancer
R140 T233 T234 compound cancer,susceptibility
R141 T234 T230 compound susceptibility,gene
R142 T235 T236 aux to,discovered
R143 T236 T230 advcl discovered,gene
R144 T237 T236 auxpass be,discovered
R145 T238 T228 punct ", ",was
R146 T239 T228 cc and,was
R147 T240 T241 auxpass was,isolated
R148 T241 T228 conj isolated,was
R149 T242 T241 prep through,isolated
R150 T243 T244 amod positional,cloning
R151 T244 T242 pobj cloning,through
R152 T245 T241 advcl using,isolated
R153 T246 T245 dobj data,using
R196 T293 T285 appos acids,sequence
R197 T294 T293 compound amino,acids
R198 T295 T286 punct ),offers
R199 T296 T297 advmod very,clue
R200 T297 T286 dobj clue,offers
R201 T298 T297 amod little,clue
R202 T299 T297 prep to,clue
R203 T300 T301 poss its,function
R204 T301 T299 pobj function,to
R205 T302 T286 punct ", ",offers
R206 T303 T304 mark although,are
R2190 T3146 T3140 conj promotes,blocks
R2191 T3147 T3148 compound RAD51,BRCA2
R2193 T3149 T3148 punct –,BRCA2
R2194 T3148 T3150 compound BRCA2,interaction
R2195 T3151 T3132 punct .,needed
R2196 T3154 T3155 nsubj experiments,reveal
R2197 T3150 T3146 dobj interaction,promotes
R2198 T3153 T3154 amod Such,experiments
R2199 T3156 T3155 aux could,reveal
R2200 T3158 T3159 amod additional,roles
R2201 T3159 T3155 dobj roles,reveal
R2202 T3157 T3155 advmod also,reveal
R2203 T3161 T3160 pobj BRCA2,for
R2204 T3162 T3159 prep in,roles
R2205 T3160 T3159 prep for,roles
R2206 T3164 T3162 pobj repair,in
R2207 T3163 T3164 amod recombinational,repair
R2208 T3169 T3168 punct ", ",promise
R2209 T3165 T3155 punct .,reveal
R2210 T3167 T3168 advmod However,promise
R2211 T3171 T3168 nsubj obstacles,promise
R2212 T3172 T3171 amod technical,obstacles
R2213 T3170 T3171 amod several,obstacles
R2214 T3174 T3171 prep including,obstacles
R2215 T3175 T3176 det the,size
R2216 T3173 T3171 punct ", ",obstacles
R2217 T3177 T3176 amod large,size
R2218 T3178 T3176 prep of,size
R2219 T3176 T3174 pobj size,including
R2220 T3180 T3168 punct ", ",promise
R2221 T3181 T3182 aux to,make
R2222 T3182 T3168 xcomp make,promise
R2223 T3179 T3178 pobj BRCA2,of
R2224 T3184 T3185 nsubj characterization,difficult
R2225 T3185 T3182 ccomp difficult,make
R2226 T3183 T3184 amod biochemical,characterization
R2227 T3187 T3188 compound BRCA2,function
R2228 T3186 T3184 prep of,characterization
R2229 T3189 T3168 punct .,promise
R2231 T3188 T3186 pobj function,of