PMC:1382200 / 7070-16444 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T22839	6909-6910	.	denotes	.
T22894	7079-7083	JJ	denotes	wild
T22893	7076-7078	NN	denotes	bp
T22892	7072-7075	CD	denotes	429
T22891	7089-7095	NN	denotes	signal
T22890	7068-7071	DT	denotes	the
T22889	7065-7067	IN	denotes	of
T22888	7057-7064	NN	denotes	absence
T22887	7053-7056	DT	denotes	the
T22886	7050-7052	IN	denotes	by
T22885	7043-7049	VBN	denotes	marked
T22884	7041-7043	,	denotes	,
T22883	7040-7041	SYM	denotes	-
T22882	7039-7040	HYPH	denotes	/
T22881	7038-7039	SYM	denotes	-
T22880	7033-7038	NN	denotes	Capn2
T22879	7027-7028	CD	denotes	4
T22878	7026-7027	SYM	denotes	#
T22877	7019-7025	NN	denotes	embryo
T22876	7029-7032	VBD	denotes	was
T22875	7013-7018	IN	denotes	while
T22874	7011-7012	SYM	denotes	-
T22873	7010-7011	HYPH	denotes	/
T22872	7009-7010	SYM	denotes	+
T22871	7004-7009	NN	denotes	Capn2
T22870	6997-6998	CD	denotes	6
T22869	6993-6996	CC	denotes	and
T22868	6991-6993	,	denotes	,
T22867	6990-6991	CD	denotes	5
T22866	6988-6990	,	denotes	,
T22865	6987-6988	CD	denotes	3
T22864	6985-6987	,	denotes	,
T22863	6984-6985	CD	denotes	2
T22862	6982-6984	,	denotes	,
T22861	6999-7003	VBD	denotes	were
T22860	6980-6981	SYM	denotes	#
T22859	6981-6982	CD	denotes	1
T22858	6972-6979	NNS	denotes	Embryos
T22857	6971-7096	sentence	denotes	Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal.
T22856	6970-6971	.	denotes	.
T22855	6962-6964	VBZ	denotes	is
T22854	6954-6961	NNS	denotes	embryos
T22853	6948-6949	HYPH	denotes	-
T22852	6949-6953	NN	denotes	cell
T22851	6947-6948	CD	denotes	8
T22850	6944-6946	IN	denotes	of
T22849	6933-6943	NN	denotes	genotyping
T22848	6929-6932	DT	denotes	the
T22847	6926-6928	IN	denotes	of
T22846	6918-6925	NN	denotes	example
T22845	6915-6917	DT	denotes	An
T22844	6913-6914	-RRB-	denotes	)
T22843	6965-6970	VBN	denotes	shown
T22842	6912-6913	LS	denotes	B
T22841	6911-6912	-LRB-	denotes	(
T22840	6910-6971	sentence	denotes	(B) An example of the genotyping of 8-cell embryos is shown.
T22838	6896-6902	NN	denotes	mutant
T22837	6893-6895	NN	denotes	bp
T22836	6889-6892	CD	denotes	389
T22835	6903-6909	NN	denotes	signal
T22834	6885-6888	DT	denotes	the
T22833	6882-6884	IN	denotes	of
T22832	6874-6881	NN	denotes	absence
T22831	6870-6873	DT	denotes	the
T22830	6867-6869	IN	denotes	by
T22829	6859-6866	VBN	denotes	denoted
T22828	6857-6859	,	denotes	,
T22827	6856-6857	SYM	denotes	+
T22826	6855-6856	HYPH	denotes	/
T22825	6854-6855	SYM	denotes	+
T22824	6849-6854	NN	denotes	Capn2
T22823	6842-6843	CD	denotes	7
T22822	6841-6842	SYM	denotes	#
T22821	6837-6840	CC	denotes	and
T22820	6834-6835	SYM	denotes	#
T22819	6835-6836	CD	denotes	3
T22818	6826-6833	NNS	denotes	embryos
T22817	6844-6848	VBD	denotes	were
T22816	6818-6825	IN	denotes	whereas
T22815	6816-6817	SYM	denotes	-
T22814	6815-6816	HYPH	denotes	/
T22813	6814-6815	SYM	denotes	+
T22812	6809-6814	NN	denotes	Capn2
T22811	6802-6803	CD	denotes	6
T22810	6798-6801	CC	denotes	and
T22809	6796-6798	,	denotes	,
T22808	6795-6796	CD	denotes	5
T22807	6793-6795	,	denotes	,
T22806	6792-6793	CD	denotes	4
T22805	6790-6792	,	denotes	,
T22804	6789-6790	CD	denotes	2
T22803	6787-6789	,	denotes	,
T22802	6804-6808	VBD	denotes	were
T22801	6785-6786	SYM	denotes	#
T22800	6786-6787	CD	denotes	1
T22799	6777-6784	NNS	denotes	Embryos
T22798	6776-6910	sentence	denotes	Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal.
T22797	6775-6776	.	denotes	.
T22796	6762-6767	NN	denotes	stage
T22795	6768-6775	NNS	denotes	embryos
T22794	6751-6761	NN	denotes	blastocyst
T22793	6748-6750	IN	denotes	of
T22792	6737-6747	NN	denotes	genotyping
T22791	6733-6736	DT	denotes	the
T22790	6730-6732	IN	denotes	of
T22789	6707-6721	JJ	denotes	representative
T22788	6705-6706	DT	denotes	A
T22787	6703-6704	-RRB-	denotes	)
T22786	6722-6729	NN	denotes	example
T22785	6702-6703	LS	denotes	A
T22784	6701-6702	-LRB-	denotes	(
T22783	6700-6776	sentence	denotes	(A) A representative example of the genotyping of blastocyst stage embryos.
T22782	6699-6700	.	denotes	.
T22781	6683-6691	JJ	denotes	internal
T22780	6692-6699	NN	denotes	control
T22779	6679-6682	DT	denotes	the
T22778	6675-6678	IN	denotes	for
T22777	6672-6674	NN	denotes	bp
T22776	6668-6671	CD	denotes	213
T22775	6664-6667	CC	denotes	and
T22774	6662-6664	,	denotes	,
T22773	6649-6655	NN	denotes	mutant
T22772	6656-6662	NN	denotes	allele
T22771	6645-6648	DT	denotes	the
T22770	6641-6644	IN	denotes	for
T22769	6638-6640	NN	denotes	bp
T22768	6634-6637	CD	denotes	389
T22767	6632-6634	,	denotes	,
T22766	6620-6621	HYPH	denotes	-
T22765	6621-6625	NN	denotes	type
T22764	6616-6620	JJ	denotes	wild
T22763	6626-6632	NN	denotes	allele
T22762	6612-6615	DT	denotes	the
T22761	6608-6611	IN	denotes	for
T22760	6605-6607	NN	denotes	bp
T22759	6601-6604	CD	denotes	429
T22758	6596-6600	VBD	denotes	were
T22757	6581-6586	JJ	denotes	final
T22756	6587-6595	NNS	denotes	products
T22755	6577-6580	DT	denotes	The
T22754	6576-6700	sentence	denotes	The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control.
T22753	6575-6576	.	denotes	.
T22752	6568-6575	NNS	denotes	alleles
T22751	6563-6567	DT	denotes	both
T22750	6560-6562	IN	denotes	in
T22749	6551-6553	VBZ	denotes	is
T22748	6554-6559	VBN	denotes	found
T22747	6545-6550	WDT	denotes	which
T22746	6543-6545	,	denotes	,
T22745	6527-6536	NN	denotes	targeting
T22744	6537-6543	NN	denotes	vector
T22743	6523-6526	DT	denotes	the
T22742	6520-6522	IN	denotes	of
T22741	6514-6515	-RRB-	denotes	)
T22740	6506-6514	JJ	denotes	upstream
T22739	6505-6506	-LRB-	denotes	(
T22738	6499-6504	JJ	denotes	short
T22737	6516-6519	NN	denotes	arm
T22736	6495-6498	DT	denotes	the
T22735	6492-6494	IN	denotes	in
T22734	6484-6491	VBN	denotes	located
T22733	6482-6484	,	denotes	,
T22732	6466-6473	NN	denotes	control
T22731	6457-6465	JJ	denotes	internal
T22730	6474-6482	NN	denotes	sequence
T22729	6454-6456	DT	denotes	an
T22728	6449-6453	IN	denotes	with
T22727	6431-6435	VBD	denotes	were
T22726	6423-6430	NNS	denotes	alleles
T22725	6416-6422	NN	denotes	mutant
T22724	6413-6415	CC	denotes	or
T22723	6407-6408	HYPH	denotes	-
T22722	6403-6407	JJ	denotes	wild
T22721	6399-6402	DT	denotes	the
T22720	6408-6412	NN	denotes	type
T22719	6392-6398	CC	denotes	either
T22718	6389-6391	IN	denotes	in
T22717	6377-6388	RB	denotes	exclusively
T22716	6371-6376	VBN	denotes	found
T22715	6352-6361	NNS	denotes	reactions
T22714	6362-6370	NNS	denotes	segments
T22713	6343-6351	JJ	denotes	separate
T22712	6436-6448	VBN	denotes	co-amplified
T22711	6340-6342	IN	denotes	In
T22710	6339-6576	sentence	denotes	In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles.
T22709	6338-6339	.	denotes	.
T22708	6337-6338	NN	denotes	K
T22707	6326-6336	NN	denotes	proteinase
T22706	6321-6325	IN	denotes	with
T22705	6312-6320	VBN	denotes	digested
T22704	6307-6311	RB	denotes	then
T22703	6303-6306	CC	denotes	and
T22702	6294-6302	NNS	denotes	oviducts
T22701	6290-6293	DT	denotes	the
T22700	6285-6289	IN	denotes	from
T22699	6272-6276	VBD	denotes	were
T22698	6270-6271	-RRB-	denotes	)
T22697	6266-6270	NN	denotes	E2.5
T22696	6265-6266	-LRB-	denotes	(
T22695	6277-6284	VBN	denotes	flushed
T22694	6251-6252	HYPH	denotes	-
T22693	6252-6256	NN	denotes	cell
T22692	6250-6251	CD	denotes	8
T22691	6247-6249	CC	denotes	or
T22690	6245-6246	-RRB-	denotes	)
T22689	6241-6245	NN	denotes	E3.5
T22688	6240-6241	-LRB-	denotes	(
T22687	6257-6264	NNS	denotes	embryos
T22686	6229-6239	NN	denotes	Blastocyst
T22685	6228-6339	sentence	denotes	Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K.
T22684	6227-6228	.	denotes	.
T22683	6222-6227	NNS	denotes	plugs
T22665	6126-6131	NN	denotes	Capn2
T22664	6125-6228	sentence	denotes	Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs.
T22663	6124-6125	.	denotes	.
T22662	6123-6124	CD	denotes	4
T22661	6116-6122	NN	denotes	Figure
T22660	6113-6115	IN	denotes	in
T22659	6103-6112	VBN	denotes	described
T22658	6100-6102	IN	denotes	as
T22657	6087-6099	NN	denotes	implantation
T22656	6084-6086	IN	denotes	to
T22655	6078-6083	JJ	denotes	prior
T22654	6070-6077	NNS	denotes	embryos
T22653	6061-6069	VB	denotes	genotype
T22652	6058-6060	TO	denotes	to
T22651	6049-6052	VBD	denotes	was
T22650	6053-6057	VBN	denotes	used
T22649	6036-6039	NN	denotes	PCR
T22648	6029-6035	VBN	denotes	nested
T22647	6040-6048	NN	denotes	strategy
T22646	6027-6028	DT	denotes	A
T22645	6026-6125	sentence	denotes	A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4.
T22644	6025-6026	.	denotes	.
T22643	6018-6025	NNS	denotes	embryos
T22642	6001-6017	JJ	denotes	pre-implantation
T22641	5998-6000	IN	denotes	of
T22640	5987-5997	NN	denotes	Genotyping
T22907	7136-7137	.	denotes	.
T22906	7123-7129	NN	denotes	weight
T22905	7113-7122	JJ	denotes	molecular
T22904	7130-7136	NN	denotes	marker
T22903	7109-7112	DT	denotes	the
T22902	7099-7100	-RRB-	denotes	)
T22901	7101-7108	VBZ	denotes	denotes
T22900	7098-7099	NN	denotes	M
T22899	7097-7098	-LRB-	denotes	(
T22682	6214-6221	JJ	denotes	vaginal
T22681	6211-6213	IN	denotes	of
T22680	6200-6210	NN	denotes	appearance
T22679	6196-6199	DT	denotes	the
T22678	6193-6195	IN	denotes	by
T22677	6167-6180	NN	denotes	fertilization
T22676	6164-6166	IN	denotes	of
T22675	6181-6192	VBN	denotes	established
T22674	6159-6163	NN	denotes	date
T22673	6155-6158	DT	denotes	the
T22672	6151-6154	CC	denotes	and
T22671	6140-6144	VBD	denotes	were
T22670	6145-6150	VBN	denotes	mated
T22669	6133-6134	SYM	denotes	-
T22668	6132-6133	HYPH	denotes	/
T22667	6131-6132	SYM	denotes	+
T22666	6135-6139	NNS	denotes	mice
T22084	5975-5976	.	denotes	.
T22083	5962-5968	NN	denotes	mutant
T22082	5969-5975	NN	denotes	allele
T22081	5958-5961	DT	denotes	the
T22080	5955-5957	IN	denotes	in
T22079	5941-5942	HYPH	denotes	-
T22078	5942-5945	NN	denotes	Neo
T22077	5938-5941	NN	denotes	PGK
T22076	5946-5954	NN	denotes	cassette
T22075	5934-5937	DT	denotes	the
T22074	5931-5933	IN	denotes	by
T22073	5919-5921	VBZ	denotes	is
T22072	5922-5930	VBN	denotes	replaced
T22071	5914-5918	WDT	denotes	that
T22070	5901-5902	HYPH	denotes	-
T22069	5902-5906	NN	denotes	type
T22068	5897-5901	JJ	denotes	wild
T22067	5907-5913	NN	denotes	allele
T22066	5893-5896	DT	denotes	the
T22065	5890-5892	IN	denotes	of
T22064	5882-5889	NN	denotes	segment
T22063	5878-5881	DT	denotes	the
T22062	5867-5877	VBZ	denotes	delineates
T22061	5846-5856	JJ	denotes	horizontal
T22060	5844-5846	,	denotes	,
T22059	5840-5844	JJ	denotes	grey
T22058	5857-5866	NN	denotes	rectangle
T22057	5838-5839	DT	denotes	A
T22056	5837-5976	sentence	denotes	A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele.
T22055	5836-5837	.	denotes	.
T22054	5831-5832	-RRB-	denotes	)
T22053	5821-5831	JJ	denotes	downstream
T22052	5820-5821	-LRB-	denotes	(
T22051	5815-5819	JJ	denotes	long
T22050	5833-5836	NN	denotes	arm
T22049	5811-5814	DT	denotes	the
T22048	5808-5810	IN	denotes	of
T22047	5802-5803	SYM	denotes	'
T22046	5801-5802	CD	denotes	5
T22045	5804-5807	NN	denotes	end
T22044	5797-5800	DT	denotes	the
T22043	5793-5796	CC	denotes	and
T22042	5783-5788	JJ	denotes	short
T22041	5789-5792	NN	denotes	arm
T22040	5779-5782	DT	denotes	the
T22039	5776-5778	IN	denotes	of
T22038	5765-5775	NNS	denotes	boundaries
T22037	5761-5764	DT	denotes	the
T22036	5739-5747	JJ	denotes	vertical
T22035	5748-5753	NNS	denotes	lines
T22034	5734-5738	JJ	denotes	thin
T22033	5754-5760	VBP	denotes	denote
T22032	5728-5733	IN	denotes	while
T22031	5726-5728	,	denotes	,
T22030	5706-5716	JJ	denotes	horizontal
T22029	5704-5706	,	denotes	,
T22028	5700-5704	JJ	denotes	open
T22027	5717-5726	NN	denotes	rectangle
T22026	5697-5699	DT	denotes	an
T22025	5694-5696	IN	denotes	by
T22024	5680-5681	HYPH	denotes	-
T22023	5681-5684	NN	denotes	Neo
T22022	5677-5680	NN	denotes	PGK
T22021	5673-5676	DT	denotes	the
T22020	5671-5673	,	denotes	,
T22019	5652-5660	JJ	denotes	vertical
T22018	5650-5652	,	denotes	,
T22017	5661-5671	NNS	denotes	rectangles
T22016	5646-5650	JJ	denotes	open
T22015	5643-5645	IN	denotes	by
T22014	5685-5693	NN	denotes	cassette
T22013	5627-5630	VBP	denotes	are
T22012	5631-5642	VBN	denotes	represented
T22011	5621-5626	NNS	denotes	Exons
T22010	5620-5837	sentence	denotes	Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm.
T22009	5619-5620	.	denotes	.
T22008	5604-5609	CD	denotes	three
T22007	5610-5619	NNS	denotes	reactions
T22006	5600-5603	DT	denotes	the
T22005	5597-5599	IN	denotes	of
T22004	5592-5596	DT	denotes	each
T22003	5588-5591	IN	denotes	for
T22002	5581-5582	-RRB-	denotes	)
T22001	5575-5581	VBN	denotes	nested
T22000	5574-5575	-LRB-	denotes	(
T21999	5583-5587	NNS	denotes	sets
T21998	5570-5573	CD	denotes	two
T21997	5567-5569	IN	denotes	in
T21996	5554-5557	VBP	denotes	are
T21995	5552-5554	,	denotes	,
T21994	5543-5552	NNS	denotes	triangles
T21993	5540-5542	IN	denotes	by
T21992	5528-5539	VBN	denotes	represented
T21991	5526-5528	,	denotes	,
T21990	5558-5566	VBN	denotes	depicted
T21989	5519-5526	NNS	denotes	primers
T21988	5515-5518	DT	denotes	The
T21987	5514-5620	sentence	denotes	The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions.
T21986	5513-5514	.	denotes	.
T21985	5504-5505	HYPH	denotes	-
T21984	5505-5513	JJ	denotes	specific
T21983	5498-5504	NN	denotes	allele
T21982	5475-5484	NN	denotes	antisense
T21981	5485-5492	NNS	denotes	primers
T21980	5471-5474	DT	denotes	the
T21979	5493-5497	VBD	denotes	were
T21978	5463-5470	IN	denotes	whereas
T21977	5461-5463	,	denotes	,
T21976	5452-5461	NNS	denotes	reactions
T21975	5447-5451	DT	denotes	both
T21974	5444-5446	IN	denotes	in
T21973	5432-5433	''	denotes	'
T21972	5434-5443	NNS	denotes	sequences
T21971	5422-5432	JJ	denotes	diagnostic
T21970	5421-5422	``	denotes	'
T21969	5413-5420	VB	denotes	amplify
T21968	5410-5412	TO	denotes	to
T21967	5400-5404	VBD	denotes	were
T21966	5405-5409	VBN	denotes	used
T21965	5386-5391	NN	denotes	sense
T21964	5381-5385	JJ	denotes	same
T21963	5392-5399	NNS	denotes	primers
T21962	5377-5380	DT	denotes	The
T21961	5376-5514	sentence	denotes	The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific.
T21960	5375-5376	.	denotes	.
T21959	5359-5367	JJ	denotes	internal
T21958	5368-5375	NN	denotes	control
T21957	5356-5358	DT	denotes	an
T21956	5353-5355	IN	denotes	as
T21955	5342-5343	''	denotes	'
T21954	5332-5342	JJ	denotes	diagnostic
T21953	5331-5332	``	denotes	'
T21952	5344-5352	NNS	denotes	products
T21951	5327-5330	DT	denotes	the
T21950	5322-5326	IN	denotes	with
T21949	5305-5308	VBD	denotes	was
T21948	5295-5300	JJ	denotes	short
T21947	5301-5304	NN	denotes	arm
T21946	5291-5294	DT	denotes	the
T21945	5284-5290	IN	denotes	within
T21944	5276-5283	VBN	denotes	located
T21943	5264-5266	NN	denotes	bp
T21942	5260-5263	CD	denotes	213
T21941	5267-5275	NN	denotes	sequence
T21940	5258-5259	DT	denotes	a
T21939	5256-5258	,	denotes	,
T21938	5247-5256	NNS	denotes	reactions
T21937	5242-5246	DT	denotes	both
T21936	5309-5321	VBN	denotes	co-amplified
T21935	5239-5241	IN	denotes	In
T21934	5238-5376	sentence	denotes	In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control.
T21933	5237-5238	.	denotes	.
T21932	5221-5230	NN	denotes	targeting
T21931	5231-5237	NN	denotes	vector
T21930	5217-5220	DT	denotes	the
T21929	5214-5216	IN	denotes	of
T21928	5208-5209	-RRB-	denotes	)
T21927	5200-5208	JJ	denotes	upstream
T21926	5199-5200	-LRB-	denotes	(
T21925	5193-5198	JJ	denotes	short
T21924	5210-5213	NN	denotes	arm
T21923	5189-5192	DT	denotes	the
T21922	5186-5188	IN	denotes	of
T21921	5180-5181	SYM	denotes	'
T21920	5179-5180	CD	denotes	3
T21919	5182-5185	NN	denotes	end
T21918	5175-5178	DT	denotes	the
T21917	5166-5174	VBG	denotes	spanning
T21916	5161-5165	DT	denotes	both
T21915	5159-5161	,	denotes	,
T21914	5146-5152	NN	denotes	mutant
T21913	5153-5159	NN	denotes	allele
T21912	5142-5145	DT	denotes	the
T21911	5137-5141	IN	denotes	from
T21910	5126-5128	NN	denotes	bp
T21909	5122-5125	CD	denotes	389
T21908	5129-5136	NN	denotes	segment
T21907	5120-5121	DT	denotes	a
T21906	5116-5119	CC	denotes	and
T21905	5103-5104	HYPH	denotes	-
T21904	5104-5108	NN	denotes	type
T21903	5099-5103	JJ	denotes	wild
T21902	5109-5115	NN	denotes	allele
T21901	5095-5098	DT	denotes	the
T21900	5090-5094	IN	denotes	from
T21899	5078-5080	NN	denotes	bp
T21898	5074-5077	CD	denotes	429
T21897	5081-5089	NN	denotes	fragment
T21896	5072-5073	DT	denotes	a
T21895	5064-5071	VB	denotes	amplify
T21894	5061-5063	TO	denotes	to
T21893	5051-5055	VBD	denotes	were
T21892	5056-5060	VBN	denotes	used
T21891	5041-5050	NNS	denotes	reactions
T21890	5032-5040	JJ	denotes	Separate
T21889	5031-5238	sentence	denotes	Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector.
T21888	5030-5031	.	denotes	.
T21887	5023-5030	NNS	denotes	embryos
T21886	5006-5022	JJ	denotes	pre-implantation
T21885	4997-5005	VB	denotes	genotype
T21884	4994-4996	TO	denotes	to
T21883	4988-4993	NN	denotes	order
T21882	4985-4987	IN	denotes	in
T21881	4971-4974	VBD	denotes	was
T21880	4958-4961	NN	denotes	PCR
T21879	4951-4957	VBN	denotes	nested
T21878	4962-4970	NN	denotes	strategy
T21877	4949-4950	DT	denotes	a
T21876	4947-4949	,	denotes	,
T21875	4931-4938	JJ	denotes	genetic
T21874	4939-4947	NN	denotes	material
T21873	4919-4930	JJ	denotes	extractable
T21872	4916-4918	IN	denotes	of
T21871	4907-4915	NN	denotes	scarcity
T21870	4903-4906	DT	denotes	the
T21869	4900-4902	IN	denotes	to
T21868	4975-4984	VBN	denotes	developed
T21867	4896-4899	IN	denotes	Due
T21866	4895-5031	sentence	denotes	Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos.
T21865	4894-4895	.	denotes	.
T21864	4887-4894	NNS	denotes	embryos
T21863	4870-4886	JJ	denotes	pre-implantation
T21862	4867-4869	IN	denotes	of
T21861	4856-4866	NN	denotes	genotyping
T21860	4852-4855	IN	denotes	for
T21859	4839-4842	NN	denotes	PCR
T21858	4843-4851	NN	denotes	strategy
T21857	4832-4838	VBN	denotes	Nested
T7801	9143-9152	NN	denotes	inability
T7800	9139-9142	DT	denotes	The
T7799	9138-9374	sentence	denotes	The inability to isolate Capn2-/- ES cells, in concert with the absence of Capn2-/- embryos beyond the 8-cell stage, suggested that m-calpain activity might be essential for cell viability or the establishment of viable ES cell clones.
T7798	9137-9138	.	denotes	.
T7797	9120-9121	HYPH	denotes	-
T7796	9121-9130	JJ	denotes	resistant
T7795	9116-9120	NN	denotes	drug
T7794	9131-9137	NNS	denotes	clones
T7793	9112-9115	CD	denotes	126
T7792	9109-9111	IN	denotes	of
T7791	9101-9108	NNS	denotes	screens
T7790	9098-9100	IN	denotes	in
T7789	9084-9088	VBD	denotes	were
T7788	9075-9077	NN	denotes	ES
T7787	9073-9074	SYM	denotes	-
T7786	9072-9073	HYPH	denotes	/
T7785	9071-9072	SYM	denotes	-
T7784	9066-9071	NN	denotes	Capn2
T7783	9078-9083	NNS	denotes	cells
T7782	9063-9065	DT	denotes	no
T7781	9061-9063	,	denotes	,
T7780	9054-9061	RB	denotes	however
T7779	9052-9054	,	denotes	,
T7778	9048-9052	NN	denotes	case
T7777	9043-9047	DT	denotes	this
T7776	9089-9097	VBN	denotes	isolated
T7775	9040-9042	IN	denotes	In
T7774	9039-9138	sentence	denotes	In this case, however, no Capn2-/- ES cells were isolated in screens of 126 drug-resistant clones.
T7773	9038-9039	.	denotes	.
T7772	9037-9038	-RRB-	denotes	]
T7771	9035-9037	CD	denotes	31
T7770	9034-9035	-LRB-	denotes	[
T7769	9025-9027	NN	denotes	ES
T7768	9023-9024	SYM	denotes	-
T7767	9022-9023	HYPH	denotes	/
T7766	9021-9022	SYM	denotes	+
T7765	9028-9033	NNS	denotes	cells
T7764	9016-9021	NN	denotes	Capn4
T7763	9013-9015	IN	denotes	of
T7762	9008-9012	NN	denotes	case
T7761	9004-9007	DT	denotes	the
T7760	9001-9003	IN	denotes	in
T7759	8984-8991	RB	denotes	readily
T7758	8992-9000	VBN	denotes	achieved
T7757	8980-8983	VBD	denotes	was
T7756	8976-8979	CC	denotes	and
T7755	8967-8969	NN	denotes	ES
T7754	8970-8975	NNS	denotes	cells
T7753	8958-8966	VBN	denotes	targeted
T7752	8955-8957	IN	denotes	to
T7751	8935-8946	RB	denotes	extensively
T7750	8930-8934	VBN	denotes	been
T7749	8926-8929	VBZ	denotes	has
T7748	8947-8954	VBN	denotes	applied
T7747	8916-8925	NN	denotes	procedure
T7746	8911-8915	DT	denotes	This
T7745	8910-9039	sentence	denotes	This procedure has been extensively applied to targeted ES cells and was readily achieved in the case of Capn4+/- ES cells [31].
T7744	8909-8910	.	denotes	.
T7743	8899-8909	NN	denotes	conversion
T7742	8894-8898	NN	denotes	gene
T7741	8891-8893	IN	denotes	by
T7740	8878-8884	NN	denotes	mutant
T7739	8885-8890	NNS	denotes	cells
T7738	8867-8877	JJ	denotes	homozygous
T7737	8858-8866	VB	denotes	generate
T7736	8855-8857	TO	denotes	to
T7735	8846-8854	NNS	denotes	attempts
T7734	8843-8845	IN	denotes	in
T7733	8835-8837	NN	denotes	mL
T7732	8834-8835	SYM	denotes	/
T7731	8838-8842	NN	denotes	G418
T7730	8832-8834	NN	denotes	mg
T7729	8830-8831	CD	denotes	2
T7728	8827-8829	IN	denotes	of
T7727	8818-8826	NN	denotes	presence
T7726	8814-8817	DT	denotes	the
T7725	8811-8813	IN	denotes	in
T7724	8801-8810	NN	denotes	selection
T7723	8794-8800	JJ	denotes	clonal
T7722	8791-8793	IN	denotes	to
T7721	8776-8780	VBD	denotes	were
T7720	8781-8790	VBN	denotes	subjected
T7719	8767-8769	NN	denotes	ES
T7718	8765-8766	SYM	denotes	-
T7717	8764-8765	HYPH	denotes	/
T7716	8763-8764	SYM	denotes	+
T7715	8770-8775	NNS	denotes	cells
T7714	8758-8763	NN	denotes	Capn2
T7713	8757-8910	sentence	denotes	Capn2+/- ES cells were subjected to clonal selection in the presence of 2 mg/mL G418 in attempts to generate homozygous mutant cells by gene conversion.
T7712	8749-8751	NN	denotes	ES
T7711	8747-8748	SYM	denotes	-
T7710	8746-8747	HYPH	denotes	/
T7709	8745-8746	SYM	denotes	-
T7708	8752-8757	NNS	denotes	cells
T7707	8740-8745	NN	denotes	Capn2
T7706	8737-8739	IN	denotes	of
T7705	8726-8736	NN	denotes	generation
T7704	8716-8725	VBN	denotes	Attempted
T7358	8707-8713	NN	denotes	father
T7357	8703-8706	DT	denotes	the
T7356	8698-8702	IN	denotes	from
T7355	8679-8685	NN	denotes	mutant
T7354	8686-8692	NN	denotes	allele
T7353	8675-8678	DT	denotes	the
T7849	9373-9374	.	denotes	.
T7848	9362-9366	NN	denotes	cell
T7847	9359-9361	NN	denotes	ES
T7846	9367-9373	NNS	denotes	clones
T7845	9352-9358	JJ	denotes	viable
T7844	9349-9351	IN	denotes	of
T7843	9335-9348	NN	denotes	establishment
T7842	9331-9334	DT	denotes	the
T7841	9328-9330	CC	denotes	or
T7840	9318-9327	NN	denotes	viability
T7839	9313-9317	NN	denotes	cell
T7838	9309-9312	IN	denotes	for
T7837	9299-9308	JJ	denotes	essential
T7836	9290-9295	MD	denotes	might
T7835	9281-9289	NN	denotes	activity
T7834	9272-9273	HYPH	denotes	-
T7833	9273-9280	NN	denotes	calpain
T7832	9271-9272	NN	denotes	m
T7831	9296-9298	VB	denotes	be
T7830	9266-9270	IN	denotes	that
T7829	9254-9256	,	denotes	,
T7828	9243-9244	HYPH	denotes	-
T7827	9244-9248	NN	denotes	cell
T7826	9242-9243	CD	denotes	8
T7825	9249-9254	NN	denotes	stage
T7824	9238-9241	DT	denotes	the
T7823	9231-9237	IN	denotes	beyond
T7822	9221-9222	SYM	denotes	-
T7821	9220-9221	HYPH	denotes	/
T7820	9219-9220	SYM	denotes	-
T7819	9223-9230	NNS	denotes	embryos
T7818	9214-9219	NN	denotes	Capn2
T7817	9211-9213	IN	denotes	of
T7816	9203-9210	NN	denotes	absence
T7815	9199-9202	DT	denotes	the
T7814	9194-9198	IN	denotes	with
T7813	9186-9193	NN	denotes	concert
T7812	9183-9185	IN	denotes	in
T7811	9181-9183	,	denotes	,
T7810	9173-9175	NN	denotes	ES
T7809	9171-9172	SYM	denotes	-
T7808	9170-9171	HYPH	denotes	/
T7807	9169-9170	SYM	denotes	-
T7806	9176-9181	NNS	denotes	cells
T7805	9164-9169	NN	denotes	Capn2
T7804	9156-9163	VB	denotes	isolate
T7803	9153-9155	TO	denotes	to
T7802	9256-9265	VBD	denotes	suggested
T7352	8693-8697	VBD	denotes	came
T7351	8670-8674	WRB	denotes	when
T7350	8668-8669	SYM	denotes	-
T7349	8667-8668	HYPH	denotes	/
T7348	8666-8667	SYM	denotes	+
T7347	8661-8666	NN	denotes	Capn2
T7346	8648-8655	NNS	denotes	females
T7345	8645-8647	IN	denotes	of
T7344	8643-8644	NN	denotes	%
T7343	8641-8643	CD	denotes	55
T7342	8637-8640	CC	denotes	and
T7341	8631-8636	NNS	denotes	males
T7340	8628-8630	IN	denotes	of
T7339	8656-8660	VBD	denotes	were
T7338	8626-8627	NN	denotes	%
T7337	8624-8626	CD	denotes	62
T7336	8620-8623	CC	denotes	and
T7335	8618-8620	,	denotes	,
T7334	8612-8618	NN	denotes	mother
T7333	8608-8611	DT	denotes	the
T7332	8603-8607	IN	denotes	from
T7331	8584-8590	NN	denotes	mutant
T7330	8591-8597	NN	denotes	allele
T7329	8580-8583	DT	denotes	the
T7328	8598-8602	VBD	denotes	came
T7327	8575-8579	WRB	denotes	when
T7326	8573-8574	SYM	denotes	-
T7325	8572-8573	HYPH	denotes	/
T7324	8571-8572	SYM	denotes	+
T7323	8566-8571	NN	denotes	Capn2
T7322	8553-8560	NNS	denotes	females
T7321	8550-8552	IN	denotes	of
T7320	8548-8549	NN	denotes	%
T7319	8546-8548	CD	denotes	69
T7318	8542-8545	CC	denotes	and
T7299	8438-8439	SYM	denotes	-
T7298	8437-8438	HYPH	denotes	/
T7297	8436-8437	SYM	denotes	+
T7296	8440-8447	NNS	denotes	animals
T7295	8431-8436	NN	denotes	Capn2
T7294	8428-8430	IN	denotes	to
T7293	8426-8427	SYM	denotes	+
T7292	8425-8426	HYPH	denotes	/
T7291	8424-8425	SYM	denotes	+
T7290	8419-8424	NN	denotes	Capn2
T7289	8416-8418	IN	denotes	of
T7288	8409-8415	NNS	denotes	ratios
T7287	8405-8408	DT	denotes	the
T7286	8397-8404	NNS	denotes	crosses
T7285	8391-8396	DT	denotes	these
T7284	8471-8479	VBN	denotes	compared
T7283	8388-8390	IN	denotes	In
T7282	8387-8714	sentence	denotes	In these crosses the ratios of Capn2+/+ to Capn2+/- animals among males or females compared well with the ratio in the combined populations; 77% of males and 69% of females were Capn2+/- when the mutant allele came from the mother, and 62% of males and 55% of females were Capn2+/- when the mutant allele came from the father.
T7281	8386-8387	.	denotes	.
T7280	8385-8386	-RRB-	denotes	)
T7279	8382-8384	CD	denotes	59
T7278	8384-8385	NN	denotes	%
T7277	8381-8382	-LRB-	denotes	(
T7276	8374-8380	NN	denotes	father
T7275	8370-8373	DT	denotes	the
T7274	8365-8369	IN	denotes	from
T7273	8357-8359	PRP	denotes	it
T7272	8360-8364	VBD	denotes	came
T7271	8352-8356	WRB	denotes	when
T7270	8349-8351	IN	denotes	to
T7269	8340-8348	VBN	denotes	compared
T7268	8338-8339	-RRB-	denotes	)
T7267	8335-8337	CD	denotes	73
T7266	8337-8338	NN	denotes	%
T7265	8334-8335	-LRB-	denotes	(
T7264	8327-8333	NN	denotes	mother
T7263	8323-8326	DT	denotes	the
T7262	8304-8310	NN	denotes	mutant
T7261	8311-8317	NN	denotes	allele
T7260	8300-8303	DT	denotes	the
T7259	8318-8322	VBD	denotes	came
T7258	8295-8299	WRB	denotes	when
T7257	8286-8289	VBD	denotes	was
T7256	8264-8265	SYM	denotes	-
T7255	8263-8264	HYPH	denotes	/
T7254	8262-8263	SYM	denotes	+
T7253	8266-8285	NN	denotes	over-representation
T7252	8257-8262	NN	denotes	Capn2
T7251	8254-8256	IN	denotes	of
T7250	8290-8294	VBN	denotes	seen
T7249	8240-8246	JJR	denotes	higher
T7248	8235-8239	RB	denotes	even
T7247	8247-8253	NN	denotes	degree
T7246	8232-8234	DT	denotes	An
T7245	8231-8387	sentence	denotes	An even higher degree of Capn2+/- over-representation was seen when the mutant allele came the mother (73%) compared to when it came from the father (59%).
T7244	8230-8231	.	denotes	.
T7243	8229-8230	-RRB-	denotes	)
T7242	8228-8229	CD	denotes	2
T7241	8222-8227	NN	denotes	Table
T7240	8221-8222	-LRB-	denotes	(
T7239	8211-8212	SYM	denotes	-
T7238	8210-8211	HYPH	denotes	/
T7237	8209-8210	SYM	denotes	+
T7236	8213-8220	NNS	denotes	animals
T7235	8204-8209	NN	denotes	Capn2
T7234	8201-8203	IN	denotes	of
T7233	8181-8189	VBN	denotes	expected
T7232	8176-8180	IN	denotes	than
T7231	8168-8175	JJR	denotes	greater
T7230	8190-8200	NN	denotes	proportion
T7229	8166-8167	DT	denotes	a
T7228	8163-8165	IN	denotes	in
T7227	8149-8153	RB	denotes	also
T7226	8139-8140	SYM	denotes	-
T7225	8138-8139	HYPH	denotes	/
T7224	8137-8138	SYM	denotes	+
T7223	8132-8137	NN	denotes	Capn2
T7222	8128-8131	CC	denotes	and
T7221	8126-8127	SYM	denotes	+
T7220	8125-8126	HYPH	denotes	/
T7219	8124-8125	SYM	denotes	+
T7218	8141-8148	NNS	denotes	animals
T7217	8119-8124	NN	denotes	Capn2
T7216	8111-8118	IN	denotes	between
T7215	8154-8162	VBD	denotes	resulted
T7214	8103-8110	NNS	denotes	Crosses
T7213	8102-8231	sentence	denotes	Crosses between Capn2+/+ and Capn2+/- animals also resulted in a greater than expected proportion of Capn2+/- animals (Table 2).
T7212	8101-8102	.	denotes	.
T7211	8100-8101	-RRB-	denotes	]
T7210	8098-8100	CD	denotes	32
T7209	8097-8098	-LRB-	denotes	[
T7208	8089-8096	JJ	denotes	extreme
T7207	8086-8088	IN	denotes	as
T7206	8082-8085	RB	denotes	not
T7205	8061-8069	NN	denotes	genotype
T7204	8070-8077	NN	denotes	skewing
T7203	8057-8060	DT	denotes	the
T7202	8078-8081	VBD	denotes	was
T7201	8050-8056	IN	denotes	though
T7200	8048-8050	,	denotes	,
T7199	8032-8042	JJ	denotes	transgenic
T7198	8026-8031	NN	denotes	Capn4
T7197	8043-8048	NNS	denotes	lines
T7196	8022-8025	DT	denotes	the
T7195	8019-8021	IN	denotes	of
T7194	8015-8018	CD	denotes	one
T7193	8012-8014	IN	denotes	in
T7192	7998-8002	RB	denotes	also
T7191	7994-7997	VBD	denotes	was
T7190	7986-7993	NNS	denotes	animals
T7189	7973-7985	JJ	denotes	heterozygous
T7188	7970-7972	IN	denotes	of
T7187	7942-7949	JJ	denotes	similar
T7186	7950-7969	NN	denotes	over-representation
T7185	7940-7941	DT	denotes	a
T7184	7938-7940	,	denotes	,
T7183	8003-8011	VBN	denotes	reported
T7182	7925-7938	RB	denotes	Interestingly
T7181	7924-8102	sentence	denotes	Interestingly, a similar over-representation of heterozygous animals was also reported in one of the Capn4 transgenic lines, though the genotype skewing was not as extreme [32].
T7180	7923-7924	.	denotes	.
T7179	7922-7923	-RRB-	denotes	)
T7178	7921-7922	CD	denotes	2
T7177	7915-7920	NN	denotes	Table
T7176	7914-7915	-LRB-	denotes	(
T7175	7889-7905	JJ	denotes	pre-implantation
T7174	7885-7888	CC	denotes	and
T7173	7867-7884	JJ	denotes	post-implantation
T7172	7906-7913	NNS	denotes	embryos
T7171	7862-7866	CC	denotes	both
T7170	7859-7861	IN	denotes	in
T7169	7851-7858	JJ	denotes	evident
T7168	7846-7850	RB	denotes	also
T7167	7832-7833	SYM	denotes	-
T7166	7831-7832	HYPH	denotes	/
T7165	7830-7831	SYM	denotes	+
T7164	7834-7841	NNS	denotes	animals
T7163	7825-7830	NN	denotes	Capn2
T7162	7842-7845	VBD	denotes	was
T7161	7805-7813	VBN	denotes	expected
T7160	7800-7804	IN	denotes	than
T7159	7793-7799	JJR	denotes	larger
T7158	7814-7824	NN	denotes	proportion
T7157	7791-7792	DT	denotes	A
T7156	7790-7924	sentence	denotes	A larger than expected proportion Capn2+/- animals was also evident in both post-implantation and pre-implantation embryos (Table 2).
T7155	7789-7790	.	denotes	.
T7154	7772-7782	NN	denotes	background
T7153	7783-7789	NN	denotes	strain
T7152	7767-7771	DT	denotes	this
T7151	7763-7766	IN	denotes	for
T7150	7756-7762	JJ	denotes	normal
T7149	7753-7755	VBZ	denotes	is
T7148	7747-7752	WDT	denotes	which
T7147	7745-7747	,	denotes	,
T7146	7739-7745	NN	denotes	litter
T7145	7735-7738	IN	denotes	per
T7144	7730-7734	NNS	denotes	pups
T7143	7726-7729	CD	denotes	six
T7142	7723-7725	IN	denotes	of
T7141	7715-7722	NN	denotes	average
T7140	7712-7714	DT	denotes	an
T7139	7707-7711	VBD	denotes	were
T7138	7701-7706	EX	denotes	there
T7137	7697-7700	CC	denotes	and
T7136	7695-7697	,	denotes	,
T7135	7676-7684	VBN	denotes	combined
T7134	7685-7695	NN	denotes	population
T7133	7672-7675	DT	denotes	the
T7132	7668-7671	IN	denotes	for
T7131	7661-7663	PRP	denotes	it
T7130	7664-7667	VBD	denotes	was
T7129	7658-7660	IN	denotes	is
T7128	7653-7657	JJ	denotes	same
T7127	7649-7652	DT	denotes	the
T7126	7637-7648	RB	denotes	essentially
T7125	7631-7632	-RRB-	denotes	)
T7124	7630-7631	NN	denotes	%
T7123	7628-7630	CD	denotes	90
T7122	7625-7627	IN	denotes	to
T7121	7621-7623	CD	denotes	13
T7120	7623-7624	NN	denotes	%
T7119	7620-7621	-LRB-	denotes	(
T7118	7612-7619	NNS	denotes	females
T7117	7609-7611	CC	denotes	or
T7116	7607-7608	-RRB-	denotes	)
T7115	7606-7607	NN	denotes	%
T7114	7604-7606	CD	denotes	82
T7113	7601-7603	IN	denotes	to
T7112	7597-7599	CD	denotes	14
T7111	7599-7600	NN	denotes	%
T7110	7596-7597	-LRB-	denotes	(
T7109	7590-7595	NNS	denotes	males
T7108	7584-7589	IN	denotes	among
T7107	7574-7575	SYM	denotes	-
T7106	7573-7574	HYPH	denotes	/
T7105	7572-7573	SYM	denotes	+
T7104	7576-7583	NNS	denotes	animals
T7103	7567-7572	NN	denotes	Capn2
T7102	7564-7566	IN	denotes	to
T7101	7562-7563	SYM	denotes	+
T7100	7561-7562	HYPH	denotes	/
T7099	7560-7561	SYM	denotes	+
T7098	7555-7560	NN	denotes	Capn2
T7097	7552-7554	IN	denotes	of
T7096	7633-7636	VBD	denotes	was
T7095	7546-7551	NN	denotes	ratio
T7094	7542-7545	DT	denotes	The
T7093	7541-7790	sentence	denotes	The ratio of Capn2+/+ to Capn2+/- animals among males (14% to 82%) or females (13% to 90%) was essentially the same is it was for the combined population, and there were an average of six pups per litter, which is normal for this background strain.
T7092	7540-7541	.	denotes	.
T7091	7527-7531	VBD	denotes	were
T7090	7517-7518	SYM	denotes	-
T7089	7516-7517	HYPH	denotes	/
T7088	7515-7516	SYM	denotes	+
T7087	7519-7526	NNS	denotes	animals
T7086	7508-7509	-RRB-	denotes	)
T7085	7503-7507	CD	denotes	88.4
T7084	7507-7508	NN	denotes	%
T7317	8536-8541	NNS	denotes	males
T7316	8533-8535	IN	denotes	of
T7315	8561-8565	VBD	denotes	were
T7314	8531-8532	NN	denotes	%
T7313	8529-8531	CD	denotes	77
T7312	8527-8528	:	denotes	;
T7311	8507-8515	VBN	denotes	combined
T7310	8516-8527	NNS	denotes	populations
T7309	8503-8506	DT	denotes	the
T7308	8500-8502	IN	denotes	in
T7307	8494-8499	NN	denotes	ratio
T7306	8490-8493	DT	denotes	the
T7305	8485-8489	IN	denotes	with
T7304	8480-8484	RB	denotes	well
T7303	8463-8470	NNS	denotes	females
T7302	8460-8462	CC	denotes	or
T7301	8454-8459	NNS	denotes	males
T7300	8448-8453	IN	denotes	among
T7083	7502-7503	-LRB-	denotes	(
T7082	7510-7515	NN	denotes	Capn2
T7081	7498-7501	CD	denotes	176
T7080	7494-7497	CC	denotes	and
T7079	7492-7493	-RRB-	denotes	)
T7078	7487-7491	CD	denotes	11.6
T7077	7491-7492	NN	denotes	%
T7076	7486-7487	-LRB-	denotes	(
T7075	7484-7485	SYM	denotes	+
T7074	7483-7484	HYPH	denotes	/
T7073	7482-7483	SYM	denotes	+
T7072	7477-7482	NN	denotes	Capn2
T7071	7474-7476	CD	denotes	23
T7070	7472-7474	,	denotes	,
T7069	7471-7472	-RRB-	denotes	)
T7068	7461-7463	CD	denotes	33
T7067	7464-7471	NNS	denotes	litters
T7066	7460-7461	-LRB-	denotes	(
T7065	7451-7459	NN	denotes	breeding
T7064	7438-7450	NN	denotes	heterozygote
T7063	7433-7437	IN	denotes	from
T7062	7425-7432	VBN	denotes	derived
T7061	7417-7424	NNS	denotes	animals
T7060	7413-7416	CD	denotes	199
T7059	7410-7412	IN	denotes	of
T7058	7404-7409	NN	denotes	group
T7057	7402-7403	DT	denotes	a
T7056	7532-7540	VBN	denotes	observed
T7055	7399-7401	IN	denotes	In
T7054	7398-7541	sentence	denotes	In a group of 199 animals derived from heterozygote breeding (33 litters), 23 Capn2+/+ (11.6%) and 176 (88.4%) Capn2+/- animals were observed.
T7053	7397-7398	.	denotes	.
T7052	7382-7391	JJ	denotes	Mendelian
T7051	7380-7381	CD	denotes	2
T7050	7379-7380	SYM	denotes	:
T7049	7378-7379	CD	denotes	1
T7048	7368-7377	VBN	denotes	predicted
T7047	7392-7397	NN	denotes	ratio
T7046	7364-7367	DT	denotes	the
T7045	7359-7363	IN	denotes	than
T7044	7354-7358	JJR	denotes	less
T7043	7340-7353	RB	denotes	substantially
T7042	7310-7322	JJ	denotes	heterozygous
T7041	7323-7335	NNS	denotes	intercrosses
T7040	7303-7309	JJ	denotes	inbred
T7039	7298-7302	IN	denotes	from
T7038	7289-7290	SYM	denotes	-
T7037	7288-7289	HYPH	denotes	/
T7036	7287-7288	SYM	denotes	+
T7035	7290-7297	NNS	denotes	animals
T7034	7282-7287	NN	denotes	Capn2
T7033	7279-7281	IN	denotes	to
T7032	7277-7278	SYM	denotes	+
T7031	7276-7277	HYPH	denotes	/
T7030	7275-7276	SYM	denotes	+
T7029	7270-7275	NN	denotes	Capn2
T7028	7267-7269	IN	denotes	of
T7027	7261-7266	NN	denotes	ratio
T7026	7257-7260	DT	denotes	the
T7025	7255-7257	,	denotes	,
T7024	7336-7339	VBD	denotes	was
T7023	7246-7255	RB	denotes	Curiously
T7022	7245-7398	sentence	denotes	Curiously, the ratio of Capn2+/+ to Capn2+/-animals from inbred heterozygous intercrosses was substantially less than the predicted 1:2 Mendelian ratio.
T7021	7244-7245	.	denotes	.
T7020	7243-7244	CD	denotes	2
T7019	7237-7242	NN	denotes	Table
T7018	7234-7236	IN	denotes	in
T7017	7224-7227	VBP	denotes	are
T4832	9-18	NN	denotes	Isolation
T4833	19-22	CC	denotes	and
T4834	23-39	NN	denotes	characterization
T4835	40-42	IN	denotes	of
T4836	43-48	NN	denotes	Capn2
T4837	49-57	VBN	denotes	targeted
T4838	66-72	NNS	denotes	clones
T4839	58-60	NN	denotes	ES
T4840	61-65	NN	denotes	cell
T4841	72-196	sentence	denotes	Two independent Capn2+/- ES cell lines, designated ES27 and ES36, were isolated from a screen of 305 drug-resistant clones.
T4842	73-76	CD	denotes	Two
T4843	106-111	NNS	denotes	lines
T4844	77-88	JJ	denotes	independent
T4845	89-94	NN	denotes	Capn2
T4846	94-95	SYM	denotes	+
T4847	95-96	HYPH	denotes	/
T4848	96-97	SYM	denotes	-
T4849	98-100	NN	denotes	ES
T4850	101-105	NN	denotes	cell
T4851	144-152	VBN	denotes	isolated
T4852	111-113	,	denotes	,
T4853	113-123	VBN	denotes	designated
T4854	124-128	NN	denotes	ES27
T4855	129-132	CC	denotes	and
T4856	133-137	NN	denotes	ES36
T4857	137-139	,	denotes	,
T4858	139-143	VBD	denotes	were
T4859	153-157	IN	denotes	from
T4860	158-159	DT	denotes	a
T4861	160-166	NN	denotes	screen
T4862	167-169	IN	denotes	of
T4863	170-173	CD	denotes	305
T4864	189-195	NNS	denotes	clones
T4865	174-178	NN	denotes	drug
T4866	179-188	JJ	denotes	resistant
T4867	178-179	HYPH	denotes	-
T4868	195-196	.	denotes	.
T4869	196-303	sentence	denotes	Correct targeting of the Capn2 locus was established both by Southern blot hybridization and PCR analysis.
T4870	197-204	JJ	denotes	Correct
T4871	205-214	NN	denotes	targeting
T4872	238-249	VBN	denotes	established
T4873	215-217	IN	denotes	of
T4874	218-221	DT	denotes	the
T4875	228-233	NN	denotes	locus
T4876	222-227	NN	denotes	Capn2
T4877	234-237	VBD	denotes	was
T4878	250-254	CC	denotes	both
T4879	255-257	IN	denotes	by
T4880	258-266	NNP	denotes	Southern
T4881	267-271	NN	denotes	blot
T4882	272-285	NN	denotes	hybridization
T4883	286-289	CC	denotes	and
T4884	290-293	NN	denotes	PCR
T4885	294-302	NN	denotes	analysis
T4886	302-303	.	denotes	.
T4887	303-517	sentence	denotes	A probe located outside the short (upstream) arm of homology hybridized to a 3.5-kb BamHI fragment of the wild-type allele and 5.3-kb BamHI fragment of the mutant allele as predicted from genomic maps (Figure 2A).
T4888	304-305	DT	denotes	A
T4889	306-311	NN	denotes	probe
T4890	365-375	VBN	denotes	hybridized
T4891	312-319	VBN	denotes	located
T4892	320-327	RB	denotes	outside
T4893	328-331	DT	denotes	the
T4894	349-352	NN	denotes	arm
T4895	332-337	JJ	denotes	short
T4896	338-339	-LRB-	denotes	(
T4897	339-347	JJ	denotes	upstream
T4898	347-348	-RRB-	denotes	)
T4899	353-355	IN	denotes	of
T4900	356-364	NN	denotes	homology
T4901	376-378	IN	denotes	to
T4902	379-380	DT	denotes	a
T4903	394-402	NN	denotes	fragment
T4904	381-384	CD	denotes	3.5
T4905	385-387	NN	denotes	kb
T4906	384-385	HYPH	denotes	-
T4907	388-393	NN	denotes	BamHI
T4908	403-405	IN	denotes	of
T4909	406-409	DT	denotes	the
T4910	420-426	NN	denotes	allele
T4911	410-414	JJ	denotes	wild
T4912	415-419	NN	denotes	type
T4913	414-415	HYPH	denotes	-
T4914	427-430	CC	denotes	and
T4915	431-434	CD	denotes	5.3
T4916	435-437	NN	denotes	kb
T4917	434-435	HYPH	denotes	-
T4918	444-452	NN	denotes	fragment
T4919	438-443	NN	denotes	BamHI
T4920	453-455	IN	denotes	of
T4921	456-459	DT	denotes	the
T4922	467-473	NN	denotes	allele
T4923	460-466	NN	denotes	mutant
T4924	474-476	IN	denotes	as
T4925	477-486	VBN	denotes	predicted
T4926	487-491	IN	denotes	from
T4927	492-499	JJ	denotes	genomic
T4928	500-504	NNS	denotes	maps
T4929	505-506	-LRB-	denotes	(
T4930	513-515	NN	denotes	2A
T4931	506-512	NN	denotes	Figure
T4932	515-516	-RRB-	denotes	)
T4933	516-517	.	denotes	.
T4934	517-742	sentence	denotes	The same probe also detected the expected 7.2-kb wild-type and 6.4-kb mutant BglII fragments, 4.9-kb wild-type and 5.7-kb mutant NcoI fragments, as well as 7.2-kb wild-type and 4.9-kb mutant BglII/AgeI fragments (not shown).
T4935	518-521	DT	denotes	The
T4936	527-532	NN	denotes	probe
T4937	522-526	JJ	denotes	same
T4938	538-546	VBD	denotes	detected
T4939	533-537	RB	denotes	also
T4940	547-550	DT	denotes	the
T4941	601-610	NNS	denotes	fragments
T4942	551-559	VBN	denotes	expected
T4943	560-563	CD	denotes	7.2
T4944	564-566	NN	denotes	kb
T4945	563-564	HYPH	denotes	-
T4946	567-571	JJ	denotes	wild
T4947	572-576	NN	denotes	type
T4948	571-572	HYPH	denotes	-
T4949	577-580	CC	denotes	and
T4950	581-584	CD	denotes	6.4
T4951	585-587	NN	denotes	kb
T4952	584-585	HYPH	denotes	-
T4953	588-594	NN	denotes	mutant
T4954	595-600	NN	denotes	BglII
T4955	610-612	,	denotes	,
T4956	612-615	CD	denotes	4.9
T4957	616-618	NN	denotes	kb
T4958	615-616	HYPH	denotes	-
T4959	652-661	NNS	denotes	fragments
T4960	619-623	JJ	denotes	wild
T4961	624-628	NN	denotes	type
T4962	623-624	HYPH	denotes	-
T4963	629-632	CC	denotes	and
T4964	633-636	CD	denotes	5.7
T4965	637-639	NN	denotes	kb
T4966	636-637	HYPH	denotes	-
T4967	640-646	NN	denotes	mutant
T4968	647-651	NN	denotes	NcoI
T4969	661-663	,	denotes	,
T4970	663-665	RB	denotes	as
T4971	671-673	IN	denotes	as
T4972	666-670	RB	denotes	well
T4973	674-677	CD	denotes	7.2
T4974	678-680	NN	denotes	kb
T4975	677-678	HYPH	denotes	-
T4976	720-729	NNS	denotes	fragments
T4977	681-685	JJ	denotes	wild
T4978	686-690	NN	denotes	type
T4979	685-686	HYPH	denotes	-
T4980	691-694	CC	denotes	and
T4981	695-698	CD	denotes	4.9
T4982	699-701	NN	denotes	kb
T4983	698-699	HYPH	denotes	-
T4984	702-708	NN	denotes	mutant
T4985	709-714	NN	denotes	BglII
T4986	715-719	NN	denotes	AgeI
T4987	714-715	HYPH	denotes	/
T4988	730-731	-LRB-	denotes	(
T4989	735-740	VBN	denotes	shown
T4990	731-734	RB	denotes	not
T4991	740-741	-RRB-	denotes	)
T4992	741-742	.	denotes	.
T4993	742-939	sentence	denotes	A probe derived from the PGK-Neo cassette recognized only the 5.3-kb BamHI fragment in Capn2+/- ES cells, suggesting that the targeting vector had integrated solely at the Capn2 locus (not shown).
T4994	743-744	DT	denotes	A
T4995	745-750	NN	denotes	probe
T4996	785-795	VBD	denotes	recognized
T4997	751-758	VBN	denotes	derived
T4998	759-763	IN	denotes	from
T4999	764-767	DT	denotes	the
T5000	776-784	NN	denotes	cassette
T5001	768-771	NN	denotes	PGK
T5002	772-775	NN	denotes	Neo
T5003	771-772	HYPH	denotes	-
T5004	796-800	RB	denotes	only
T5005	818-826	NN	denotes	fragment
T5006	801-804	DT	denotes	the
T5007	805-808	CD	denotes	5.3
T5008	809-811	NN	denotes	kb
T5009	808-809	HYPH	denotes	-
T5010	812-817	NN	denotes	BamHI
T5011	827-829	IN	denotes	in
T5012	830-835	NN	denotes	Capn2
T5013	839-841	NN	denotes	ES
T5014	835-836	SYM	denotes	+
T5015	836-837	HYPH	denotes	/
T5016	837-838	SYM	denotes	-
T5017	842-847	NNS	denotes	cells
T5018	847-849	,	denotes	,
T5019	849-859	VBG	denotes	suggesting
T5020	860-864	IN	denotes	that
T5021	890-900	VBN	denotes	integrated
T5022	865-868	DT	denotes	the
T5023	879-885	NN	denotes	vector
T5024	869-878	NN	denotes	targeting
T5025	886-889	VBD	denotes	had
T5026	901-907	RB	denotes	solely
T5027	908-910	IN	denotes	at
T5028	911-914	DT	denotes	the
T5029	921-926	NN	denotes	locus
T5030	915-920	NN	denotes	Capn2
T5031	927-928	-LRB-	denotes	(
T5032	932-937	VBN	denotes	shown
T5033	928-931	RB	denotes	not
T5034	937-938	-RRB-	denotes	)
T5035	938-939	.	denotes	.
T5036	939-1077	sentence	denotes	A PCR screening method was also established that generated a wild-type product of 2,749 bp and a 2,711 bp product from the mutant allele.
T5037	940-941	DT	denotes	A
T5038	956-962	NN	denotes	method
T5039	942-945	NN	denotes	PCR
T5040	946-955	NN	denotes	screening
T5041	972-983	VBN	denotes	established
T5042	963-966	VBD	denotes	was
T5043	967-971	RB	denotes	also
T5044	984-988	WDT	denotes	that
T5045	989-998	VBD	denotes	generated
T5046	999-1000	DT	denotes	a
T5047	1011-1018	NN	denotes	product
T5048	1001-1005	JJ	denotes	wild
T5049	1006-1010	NN	denotes	type
T5050	1005-1006	HYPH	denotes	-
T5051	1019-1021	IN	denotes	of
T5052	1022-1027	CD	denotes	2,749
T5053	1028-1030	NN	denotes	bp
T5054	1031-1034	CC	denotes	and
T5055	1035-1036	DT	denotes	a
T5056	1046-1053	NN	denotes	product
T5057	1037-1042	CD	denotes	2,711
T5058	1043-1045	NN	denotes	bp
T5059	1054-1058	IN	denotes	from
T5060	1059-1062	DT	denotes	the
T5061	1070-1076	NN	denotes	allele
T5062	1063-1069	NN	denotes	mutant
T5063	1076-1077	.	denotes	.
T5064	1077-1159	sentence	denotes	The 2,711 bp product was only evident in the two targeted cell lines (Figure 2B).
T5065	1078-1081	DT	denotes	The
T5066	1091-1098	NN	denotes	product
T5067	1082-1087	CD	denotes	2,711
T5068	1088-1090	NN	denotes	bp
T5069	1099-1102	VBD	denotes	was
T5070	1103-1107	RB	denotes	only
T5071	1108-1115	JJ	denotes	evident
T5072	1116-1118	IN	denotes	in
T5073	1119-1122	DT	denotes	the
T5074	1141-1146	NNS	denotes	lines
T5075	1123-1126	CD	denotes	two
T5076	1127-1135	VBN	denotes	targeted
T5077	1136-1140	NN	denotes	cell
T5078	1147-1148	-LRB-	denotes	(
T5079	1155-1157	NN	denotes	2B
T5080	1148-1154	NN	denotes	Figure
T5081	1157-1158	-RRB-	denotes	)
T5082	1158-1159	.	denotes	.
T6635	2455-2465	NN	denotes	Generation
T6636	2466-2468	IN	denotes	of
T6637	2469-2477	JJ	denotes	chimeric
T6638	2478-2482	NNS	denotes	mice
T6639	2483-2486	CC	denotes	and
T6640	2487-2495	NN	denotes	germline
T6641	2496-2508	NN	denotes	transmission
T6642	2509-2511	IN	denotes	of
T6643	2512-2515	DT	denotes	the
T6644	2529-2535	NN	denotes	allele
T6645	2516-2522	NN	denotes	mutant
T6646	2523-2528	NN	denotes	Capn2
T6647	2535-2643	sentence	denotes	Eight chimeric male mice were produced in morula aggregation experiments using the Capn2+/- ES27 cell line.
T6648	2536-2541	CD	denotes	Eight
T6649	2556-2560	NNS	denotes	mice
T6650	2542-2550	JJ	denotes	chimeric
T6651	2551-2555	JJ	denotes	male
T6652	2566-2574	VBN	denotes	produced
T6653	2561-2565	VBD	denotes	were
T6654	2575-2577	IN	denotes	in
T6655	2578-2584	NN	denotes	morula
T6656	2585-2596	NN	denotes	aggregation
T6657	2597-2608	NNS	denotes	experiments
T6658	2609-2614	VBG	denotes	using
T6659	2615-2618	DT	denotes	the
T6660	2638-2642	NN	denotes	line
T6661	2619-2624	NN	denotes	Capn2
T6662	2624-2625	SYM	denotes	+
T6663	2625-2626	HYPH	denotes	/
T6664	2626-2627	SYM	denotes	-
T6665	2628-2632	NN	denotes	ES27
T6666	2633-2637	NN	denotes	cell
T6667	2642-2643	.	denotes	.
T6668	2643-2746	sentence	denotes	Two of these males transmitted the Capn2+/- ES27 genotype through the germline into the F1 generation.
T6669	2644-2647	CD	denotes	Two
T6670	2663-2674	VBD	denotes	transmitted
T6671	2648-2650	IN	denotes	of
T6672	2651-2656	DT	denotes	these
T6673	2657-2662	NNS	denotes	males
T6674	2675-2678	DT	denotes	the
T6675	2693-2701	NN	denotes	genotype
T6676	2679-2684	NN	denotes	Capn2
T6677	2684-2685	SYM	denotes	+
T6678	2685-2686	HYPH	denotes	/
T6679	2686-2687	SYM	denotes	-
T6680	2688-2692	NN	denotes	ES27
T6681	2702-2709	IN	denotes	through
T6682	2710-2713	DT	denotes	the
T6683	2714-2722	NN	denotes	germline
T6684	2723-2727	IN	denotes	into
T6685	2728-2731	DT	denotes	the
T6686	2735-2745	NN	denotes	generation
T6687	2732-2734	NN	denotes	F1
T6688	2745-2746	.	denotes	.
T6689	2746-2863	sentence	denotes	Heterozygous Capn2+/- animals appeared normal, with no obvious defects in gross anatomy, reproduction, or life span.
T6690	2747-2759	JJ	denotes	Heterozygous
T6691	2769-2776	NNS	denotes	animals
T6692	2760-2765	NN	denotes	Capn2
T6693	2765-2766	SYM	denotes	+
T6694	2766-2767	HYPH	denotes	/
T6695	2767-2768	SYM	denotes	-
T6696	2777-2785	VBD	denotes	appeared
T6697	2786-2792	JJ	denotes	normal
T6698	2792-2794	,	denotes	,
T6699	2794-2798	IN	denotes	with
T6700	2799-2801	DT	denotes	no
T6701	2810-2817	NNS	denotes	defects
T6702	2802-2809	JJ	denotes	obvious
T6703	2818-2820	IN	denotes	in
T6704	2821-2826	JJ	denotes	gross
T6705	2827-2834	NN	denotes	anatomy
T6706	2834-2836	,	denotes	,
T6707	2836-2848	NN	denotes	reproduction
T6708	2848-2850	,	denotes	,
T6709	2850-2852	CC	denotes	or
T6710	2853-2857	NN	denotes	life
T6711	2858-2862	NN	denotes	span
T6712	2862-2863	.	denotes	.
T6713	2863-2961	sentence	denotes	Out of 199 weanlings from heterozygous intercrosses, no Capn2-/- progeny were detected (Table 2).
T6714	2864-2867	IN	denotes	Out
T6715	2942-2950	VBN	denotes	detected
T6716	2868-2870	IN	denotes	of
T6717	2871-2874	CD	denotes	199
T6718	2875-2884	NNS	denotes	weanlings
T6719	2885-2889	IN	denotes	from
T6720	2890-2902	JJ	denotes	heterozygous
T6721	2903-2915	NNS	denotes	intercrosses
T6722	2915-2917	,	denotes	,
T6723	2917-2919	DT	denotes	no
T6724	2929-2936	NN	denotes	progeny
T6725	2920-2925	NN	denotes	Capn2
T6726	2925-2926	SYM	denotes	-
T6727	2926-2927	HYPH	denotes	/
T6728	2927-2928	SYM	denotes	-
T6729	2937-2941	VBD	denotes	were
T6730	2951-2952	-LRB-	denotes	(
T6731	2952-2957	NN	denotes	Table
T6732	2958-2959	CD	denotes	2
T6733	2959-2960	-RRB-	denotes	)
T6734	2960-2961	.	denotes	.
T6735	2961-3053	sentence	denotes	We did not observe high rates of perinatal death, and no Capn2-/- stillborns were observed.
T6736	2962-2964	PRP	denotes	We
T6737	2973-2980	VB	denotes	observe
T6738	2965-2968	VBD	denotes	did
T6739	2969-2972	RB	denotes	not
T6740	2981-2985	JJ	denotes	high
T6741	2986-2991	NNS	denotes	rates
T6742	2992-2994	IN	denotes	of
T6743	2995-3004	JJ	denotes	perinatal
T6744	3005-3010	NN	denotes	death
T6745	3010-3012	,	denotes	,
T6746	3012-3015	CC	denotes	and
T6747	3016-3018	DT	denotes	no
T6748	3028-3038	NNS	denotes	stillborns
T6749	3019-3024	NN	denotes	Capn2
T6750	3024-3025	SYM	denotes	-
T6751	3025-3026	HYPH	denotes	/
T6752	3026-3027	SYM	denotes	-
T6753	3044-3052	VBN	denotes	observed
T6754	3039-3043	VBD	denotes	were
T6755	3052-3053	.	denotes	.
T6756	3053-3143	sentence	denotes	This suggested that Capn2-/- animals perished at some stage during embryonic development.
T6757	3054-3058	DT	denotes	This
T6758	3059-3068	VBD	denotes	suggested
T6759	3069-3073	IN	denotes	that
T6760	3091-3099	VBN	denotes	perished
T6761	3074-3079	NN	denotes	Capn2
T6762	3083-3090	NNS	denotes	animals
T6763	3079-3080	SYM	denotes	-
T6764	3080-3081	HYPH	denotes	/
T6765	3081-3082	SYM	denotes	-
T6766	3100-3102	IN	denotes	at
T6767	3103-3107	DT	denotes	some
T6768	3108-3113	NN	denotes	stage
T6769	3114-3120	IN	denotes	during
T6770	3121-3130	JJ	denotes	embryonic
T6771	3131-3142	NN	denotes	development
T6772	3142-3143	.	denotes	.
T6773	3143-3310	sentence	denotes	In an attempt to determine if embryonic death occurred at a post-implantation stage, embryos were harvested for genotyping at different times between E10.5 and E18.5.
T6774	3144-3146	IN	denotes	In
T6775	3242-3251	VBN	denotes	harvested
T6776	3147-3149	DT	denotes	an
T6777	3150-3157	NN	denotes	attempt
T6778	3158-3160	TO	denotes	to
T6779	3161-3170	VB	denotes	determine
T6780	3171-3173	IN	denotes	if
T6781	3190-3198	VBD	denotes	occurred
T6782	3174-3183	JJ	denotes	embryonic
T6783	3184-3189	NN	denotes	death
T6784	3199-3201	IN	denotes	at
T6785	3202-3203	DT	denotes	a
T6786	3222-3227	NN	denotes	stage
T6787	3204-3221	JJ	denotes	post-implantation
T6788	3227-3229	,	denotes	,
T6789	3229-3236	NNS	denotes	embryos
T6790	3237-3241	VBD	denotes	were
T6791	3252-3255	IN	denotes	for
T6792	3256-3266	NN	denotes	genotyping
T6793	3267-3269	IN	denotes	at
T6794	3270-3279	JJ	denotes	different
T6795	3280-3285	NNS	denotes	times
T6796	3286-3293	IN	denotes	between
T6797	3294-3299	NN	denotes	E10.5
T6798	3300-3303	CC	denotes	and
T6799	3304-3309	NN	denotes	E18.5
T6800	3309-3310	.	denotes	.
T6801	3310-3403	sentence	denotes	No Capn2-/- embryos were observed and no signs of embryo resorption were detected (Table 2).
T6802	3311-3313	DT	denotes	No
T6803	3323-3330	NNS	denotes	embryos
T6804	3314-3319	NN	denotes	Capn2
T6805	3319-3320	SYM	denotes	-
T6806	3320-3321	HYPH	denotes	/
T6807	3321-3322	SYM	denotes	-
T6808	3336-3344	VBN	denotes	observed
T6809	3331-3335	VBD	denotes	were
T6810	3345-3348	CC	denotes	and
T6811	3349-3351	DT	denotes	no
T6812	3352-3357	NNS	denotes	signs
T6813	3384-3392	VBN	denotes	detected
T6814	3358-3360	IN	denotes	of
T6815	3361-3367	NN	denotes	embryo
T6816	3368-3378	NN	denotes	resorption
T6817	3379-3383	VBD	denotes	were
T6818	3393-3394	-LRB-	denotes	(
T6819	3394-3399	NN	denotes	Table
T6820	3400-3401	CD	denotes	2
T6821	3401-3402	-RRB-	denotes	)
T6822	3402-3403	.	denotes	.
T6823	3403-3482	sentence	denotes	This indicated that the Capn2-/- embryos might be dying prior to implantation.
T6824	3404-3408	DT	denotes	This
T6825	3409-3418	VBD	denotes	indicated
T6826	3419-3423	IN	denotes	that
T6827	3454-3459	VBG	denotes	dying
T6828	3424-3427	DT	denotes	the
T6829	3437-3444	NNS	denotes	embryos
T6830	3428-3433	NN	denotes	Capn2
T6831	3433-3434	SYM	denotes	-
T6832	3434-3435	HYPH	denotes	/
T6833	3435-3436	SYM	denotes	-
T6834	3445-3450	MD	denotes	might
T6835	3451-3453	VB	denotes	be
T6836	3460-3465	JJ	denotes	prior
T6837	3466-3468	IN	denotes	to
T6838	3469-3481	NN	denotes	implantation
T6839	3481-3482	.	denotes	.
T6840	3482-3623	sentence	denotes	Embryos were then flushed from the oviducts of pregnant females at E2.5 or E3.5, and genotyped by means of a nested PCR strategy (Figure 4).
T6841	3483-3490	NNS	denotes	Embryos
T6842	3501-3508	VBN	denotes	flushed
T6843	3491-3495	VBD	denotes	were
T6844	3496-3500	RB	denotes	then
T6845	3509-3513	IN	denotes	from
T6846	3514-3517	DT	denotes	the
T6847	3518-3526	NNS	denotes	oviducts
T6848	3527-3529	IN	denotes	of
T6849	3530-3538	JJ	denotes	pregnant
T6850	3539-3546	NNS	denotes	females
T6851	3547-3549	IN	denotes	at
T6852	3550-3554	NN	denotes	E2.5
T6853	3555-3557	CC	denotes	or
T6854	3558-3562	NN	denotes	E3.5
T6855	3562-3564	,	denotes	,
T6856	3564-3567	CC	denotes	and
T6857	3568-3577	VBN	denotes	genotyped
T6858	3578-3580	IN	denotes	by
T6859	3581-3586	NNS	denotes	means
T6860	3587-3589	IN	denotes	of
T6861	3590-3591	DT	denotes	a
T6862	3603-3611	NN	denotes	strategy
T6863	3592-3598	VBN	denotes	nested
T6864	3599-3602	NN	denotes	PCR
T6865	3612-3613	-LRB-	denotes	(
T6866	3613-3619	NN	denotes	Figure
T6867	3620-3621	CD	denotes	4
T6868	3621-3622	-RRB-	denotes	)
T6869	3622-3623	.	denotes	.
T6870	3623-3717	sentence	denotes	Two of 90 successfully genotyped pre-implantation embryos were Capn2-/-, (Table 2; Figure 5).
T6871	3624-3627	CD	denotes	Two
T6872	3682-3686	VBD	denotes	were
T6873	3628-3630	IN	denotes	of
T6874	3631-3633	CD	denotes	90
T6875	3674-3681	NNS	denotes	embryos
T6876	3634-3646	RB	denotes	successfully
T6877	3647-3656	VBN	denotes	genotyped
T6878	3657-3673	JJ	denotes	pre-implantation
T6879	3687-3692	NN	denotes	Capn2
T6880	3692-3693	SYM	denotes	-
T6881	3693-3694	HYPH	denotes	/
T6882	3694-3695	SYM	denotes	-
T6883	3695-3697	,	denotes	,
T6884	3697-3698	-LRB-	denotes	(
T6885	3707-3713	NN	denotes	Figure
T6886	3698-3703	NN	denotes	Table
T6887	3704-3705	CD	denotes	2
T6888	3705-3706	:	denotes	;
T6889	3714-3715	CD	denotes	5
T6890	3715-3716	-RRB-	denotes	)
T6891	3716-3717	.	denotes	.
T6892	3717-3837	sentence	denotes	Both of these Capn2-/- embryos were isolated at the 8-cell stage and did not display any obvious morphological defects.
T6893	3718-3722	DT	denotes	Both
T6894	3754-3762	VBN	denotes	isolated
T6895	3723-3725	IN	denotes	of
T6896	3726-3731	DT	denotes	these
T6897	3741-3748	NNS	denotes	embryos
T6898	3732-3737	NN	denotes	Capn2
T6899	3737-3738	SYM	denotes	-
T6900	3738-3739	HYPH	denotes	/
T6901	3739-3740	SYM	denotes	-
T6902	3749-3753	VBD	denotes	were
T6903	3763-3765	IN	denotes	at
T6904	3766-3769	DT	denotes	the
T6905	3777-3782	NN	denotes	stage
T6906	3770-3771	CD	denotes	8
T6907	3772-3776	NN	denotes	cell
T6908	3771-3772	HYPH	denotes	-
T6909	3783-3786	CC	denotes	and
T6910	3787-3790	VBD	denotes	did
T6911	3795-3802	VB	denotes	display
T6912	3791-3794	RB	denotes	not
T6913	3803-3806	DT	denotes	any
T6914	3829-3836	NNS	denotes	defects
T6915	3807-3814	JJ	denotes	obvious
T6916	3815-3828	JJ	denotes	morphological
T6917	3836-3837	.	denotes	.
T6918	3837-3916	sentence	denotes	None of the 46 successfully genotyped blastocyst-staged embryos were Capn2-/-.
T6919	3838-3842	NN	denotes	None
T6920	3902-3906	VBD	denotes	were
T6921	3843-3845	IN	denotes	of
T6922	3846-3849	DT	denotes	the
T6923	3894-3901	NNS	denotes	embryos
T6924	3850-3852	CD	denotes	46
T6925	3853-3865	RB	denotes	successfully
T6926	3866-3875	VBN	denotes	genotyped
T6927	3876-3886	NN	denotes	blastocyst
T6928	3887-3893	VBN	denotes	staged
T6929	3886-3887	HYPH	denotes	-
T6930	3907-3912	NN	denotes	Capn2
T6931	3912-3913	SYM	denotes	-
T6932	3913-3914	HYPH	denotes	/
T6933	3914-3915	SYM	denotes	-
T6934	3915-3916	.	denotes	.
T6935	3916-4088	sentence	denotes	The scarcity of Capn2-deficient embryos surviving to the 8-cell stage suggested that the loss of m-calpain activity must fatally compromise the viability of early embryos.
T6936	3917-3920	DT	denotes	The
T6937	3921-3929	NN	denotes	scarcity
T6938	3987-3996	VBD	denotes	suggested
T6939	3930-3932	IN	denotes	of
T6940	3933-3938	NN	denotes	Capn2
T6941	3939-3948	JJ	denotes	deficient
T6942	3938-3939	HYPH	denotes	-
T6943	3949-3956	NNS	denotes	embryos
T6944	3957-3966	VBG	denotes	surviving
T6945	3967-3969	IN	denotes	to
T6946	3970-3973	DT	denotes	the
T6947	3981-3986	NN	denotes	stage
T6948	3974-3975	CD	denotes	8
T6949	3976-3980	NN	denotes	cell
T6950	3975-3976	HYPH	denotes	-
T6951	3997-4001	IN	denotes	that
T6952	4046-4056	VB	denotes	compromise
T6953	4002-4005	DT	denotes	the
T6954	4006-4010	NN	denotes	loss
T6955	4011-4013	IN	denotes	of
T6956	4014-4015	NN	denotes	m
T6957	4016-4023	NN	denotes	calpain
T6958	4015-4016	HYPH	denotes	-
T6959	4024-4032	NN	denotes	activity
T6960	4033-4037	MD	denotes	must
T6961	4038-4045	RB	denotes	fatally
T6962	4057-4060	DT	denotes	the
T6963	4061-4070	NN	denotes	viability
T6964	4071-4073	IN	denotes	of
T6965	4074-4079	JJ	denotes	early
T6966	4080-4087	NNS	denotes	embryos
T6967	4087-4088	.	denotes	.
T6968	4088-4273	sentence	denotes	Furthermore, it is possible that persistence of some maternally derived mRNA transcript or protein might have allowed a small number of Capn2-/- embryos to survive to the morula-stage.
T6969	4089-4100	RB	denotes	Furthermore
T6970	4105-4107	VBZ	denotes	is
T6971	4100-4102	,	denotes	,
T6972	4102-4104	PRP	denotes	it
T6973	4108-4116	JJ	denotes	possible
T6974	4117-4121	IN	denotes	that
T6975	4199-4206	VBN	denotes	allowed
T6976	4122-4133	NN	denotes	persistence
T6977	4134-4136	IN	denotes	of
T6978	4137-4141	DT	denotes	some
T6979	4166-4176	NN	denotes	transcript
T6980	4142-4152	RB	denotes	maternally
T6981	4153-4160	VBN	denotes	derived
T6982	4161-4165	NN	denotes	mRNA
T6983	4177-4179	CC	denotes	or
T6984	4180-4187	NN	denotes	protein
T6985	4188-4193	MD	denotes	might
T7359	8713-8714	.	denotes	.
T21151	1170-1186	NN	denotes	Characterization
T21152	1187-1189	IN	denotes	of
T21153	1190-1198	VBN	denotes	targeted
T21154	1207-1212	NNS	denotes	lines
T21155	1199-1201	NN	denotes	ES
T21156	1202-1206	NN	denotes	cell
T21157	1212-1213	.	denotes	.
T21158	1213-1301	sentence	denotes	(A) Targeted disruption of the Capn2 locus was detected initially by Southern blotting.
T21159	1214-1215	-LRB-	denotes	(
T21160	1215-1216	LS	denotes	A
T21161	1261-1269	VBN	denotes	detected
T21162	1216-1217	-RRB-	denotes	)
T21163	1218-1226	VBN	denotes	Targeted
T21164	1227-1237	NN	denotes	disruption
T21165	1238-1240	IN	denotes	of
T21166	1241-1244	DT	denotes	the
T21167	1251-1256	NN	denotes	locus
T21168	1245-1250	NN	denotes	Capn2
T21169	1257-1260	VBD	denotes	was
T21170	1270-1279	RB	denotes	initially
T21171	1280-1282	IN	denotes	by
T21172	1283-1291	NNP	denotes	Southern
T21173	1292-1300	NN	denotes	blotting
T21174	1300-1301	.	denotes	.
T21175	1301-1526	sentence	denotes	Membranes were blotted with BamHI-digested genomic DNA extracted from ES cells and hybridized with a DIG-labeled 823 bp BamHI/HindIII fragment located immediately upstream of the short arm of the targeting vector (Figure 1).
T21176	1302-1311	NNS	denotes	Membranes
T21177	1317-1324	VBN	denotes	blotted
T21178	1312-1316	VBD	denotes	were
T21179	1325-1329	IN	denotes	with
T21180	1330-1335	NN	denotes	BamHI
T21181	1336-1344	VBN	denotes	digested
T21182	1335-1336	HYPH	denotes	-
T21183	1353-1356	NN	denotes	DNA
T21184	1345-1352	JJ	denotes	genomic
T21185	1357-1366	VBN	denotes	extracted
T21186	1367-1371	IN	denotes	from
T21187	1372-1374	NN	denotes	ES
T21188	1375-1380	NNS	denotes	cells
T21189	1381-1384	CC	denotes	and
T21190	1385-1395	VBD	denotes	hybridized
T21191	1396-1400	IN	denotes	with
T21192	1401-1402	DT	denotes	a
T21193	1436-1444	NN	denotes	fragment
T21194	1403-1406	NN	denotes	DIG
T21195	1407-1414	VBN	denotes	labeled
T21196	1406-1407	HYPH	denotes	-
T21197	1415-1418	CD	denotes	823
T21198	1419-1421	NN	denotes	bp
T21199	1422-1427	NN	denotes	BamHI
T21200	1428-1435	NN	denotes	HindIII
T21201	1427-1428	HYPH	denotes	/
T21202	1445-1452	VBN	denotes	located
T21203	1453-1464	RB	denotes	immediately
T21204	1465-1473	IN	denotes	upstream
T21205	1474-1476	IN	denotes	of
T21206	1477-1480	DT	denotes	the
T21207	1487-1490	NN	denotes	arm
T21208	1481-1486	JJ	denotes	short
T21209	1491-1493	IN	denotes	of
T21210	1494-1497	DT	denotes	the
T21211	1508-1514	NN	denotes	vector
T21212	1498-1507	NN	denotes	targeting
T21213	1515-1516	-LRB-	denotes	(
T21214	1516-1522	NN	denotes	Figure
T21215	1523-1524	CD	denotes	1
T21216	1524-1525	-RRB-	denotes	)
T21217	1525-1526	.	denotes	.
T21218	1526-1730	sentence	denotes	A 3.5-kb BamHI fragment corresponding to the wild-type allele was present in all cells, whereas a 5.3-kb fragment from the mutant allele was detected in two targeted cell lines, designated ES27 and ES36.
T21219	1527-1528	DT	denotes	A
T21220	1542-1550	NN	denotes	fragment
T21221	1529-1532	CD	denotes	3.5
T21222	1533-1535	NN	denotes	kb
T21223	1532-1533	HYPH	denotes	-
T21224	1536-1541	NN	denotes	BamHI
T21225	1589-1592	VBD	denotes	was
T21226	1551-1564	VBG	denotes	corresponding
T21227	1565-1567	IN	denotes	to
T21228	1568-1571	DT	denotes	the
T21229	1582-1588	NN	denotes	allele
T21230	1572-1576	JJ	denotes	wild
T21231	1577-1581	NN	denotes	type
T21232	1576-1577	HYPH	denotes	-
T21233	1593-1600	JJ	denotes	present
T21234	1601-1603	IN	denotes	in
T21235	1604-1607	DT	denotes	all
T21236	1608-1613	NNS	denotes	cells
T21237	1613-1615	,	denotes	,
T21238	1615-1622	IN	denotes	whereas
T21239	1668-1676	VBN	denotes	detected
T21240	1623-1624	DT	denotes	a
T21241	1632-1640	NN	denotes	fragment
T21242	1625-1628	CD	denotes	5.3
T21243	1629-1631	NN	denotes	kb
T21244	1628-1629	HYPH	denotes	-
T21245	1641-1645	IN	denotes	from
T21246	1646-1649	DT	denotes	the
T21247	1657-1663	NN	denotes	allele
T21248	1650-1656	NN	denotes	mutant
T21249	1664-1667	VBD	denotes	was
T21250	1677-1679	IN	denotes	in
T21251	1680-1683	CD	denotes	two
T21252	1698-1703	NNS	denotes	lines
T21253	1684-1692	VBN	denotes	targeted
T21254	1693-1697	NN	denotes	cell
T21255	1703-1705	,	denotes	,
T21256	1705-1715	VBN	denotes	designated
T21257	1716-1720	NN	denotes	ES27
T21258	1721-1724	CC	denotes	and
T21259	1725-1729	NN	denotes	ES36
T21260	1729-1730	.	denotes	.
T21261	1730-1911	sentence	denotes	(B) PCR genotyping was carried out with two separate reactions designed to amplify either a 2,748 bp segment from the wild-type allele or a 2,711 bp segment from the mutant allele.
T21262	1731-1732	-LRB-	denotes	(
T21263	1732-1733	LS	denotes	B
T21264	1754-1761	VBN	denotes	carried
T21265	1733-1734	-RRB-	denotes	)
T21266	1735-1738	NN	denotes	PCR
T21267	1739-1749	NN	denotes	genotyping
T21268	1750-1753	VBD	denotes	was
T21269	1762-1765	RP	denotes	out
T21270	1766-1770	IN	denotes	with
T21271	1771-1774	CD	denotes	two
T21272	1784-1793	NNS	denotes	reactions
T21273	1775-1783	JJ	denotes	separate
T21274	1794-1802	VBN	denotes	designed
T21275	1803-1805	TO	denotes	to
T21276	1806-1813	VB	denotes	amplify
T21277	1814-1820	CC	denotes	either
T21278	1832-1839	NN	denotes	segment
T21279	1821-1822	DT	denotes	a
T21280	1823-1828	CD	denotes	2,748
T21281	1829-1831	NN	denotes	bp
T21282	1840-1844	IN	denotes	from
T21283	1845-1848	DT	denotes	the
T21284	1859-1865	NN	denotes	allele
T21285	1849-1853	JJ	denotes	wild
T21286	1854-1858	NN	denotes	type
T21287	1853-1854	HYPH	denotes	-
T21288	1866-1868	CC	denotes	or
T21289	1869-1870	DT	denotes	a
T21290	1880-1887	NN	denotes	segment
T21291	1871-1876	CD	denotes	2,711
T21292	1877-1879	NN	denotes	bp
T21293	1888-1892	IN	denotes	from
T21294	1893-1896	DT	denotes	the
T21295	1904-1910	NN	denotes	allele
T21296	1897-1903	NN	denotes	mutant
T21297	1910-1911	.	denotes	.
T21298	1911-2069	sentence	denotes	Both reactions used a common sense primer located in intron 4, outside the short arm of the targeting vector, and distinct allele-specific antisense primers.
T21299	1912-1916	DT	denotes	Both
T21300	1917-1926	NNS	denotes	reactions
T21301	1927-1931	VBD	denotes	used
T21302	1932-1933	DT	denotes	a
T21303	1947-1953	NN	denotes	primer
T21304	1934-1940	JJ	denotes	common
T21305	1941-1946	NN	denotes	sense
T21306	1954-1961	VBN	denotes	located
T21307	1962-1964	IN	denotes	in
T21308	1965-1971	NN	denotes	intron
T21309	1972-1973	CD	denotes	4
T21310	1973-1975	,	denotes	,
T21311	1975-1982	IN	denotes	outside
T21312	1983-1986	DT	denotes	the
T21313	1993-1996	NN	denotes	arm
T21314	1987-1992	JJ	denotes	short
T21315	1997-1999	IN	denotes	of
T21316	2000-2003	DT	denotes	the
T21317	2014-2020	NN	denotes	vector
T21318	2004-2013	NN	denotes	targeting
T21319	2020-2022	,	denotes	,
T21320	2022-2025	CC	denotes	and
T21321	2026-2034	JJ	denotes	distinct
T21322	2061-2068	NNS	denotes	primers
T21323	2035-2041	NN	denotes	allele
T21324	2042-2050	JJ	denotes	specific
T21325	2041-2042	HYPH	denotes	-
T21326	2051-2060	NN	denotes	antisense
T21327	2068-2069	.	denotes	.
T21328	2069-2262	sentence	denotes	The reaction to detect the wild-type allele used an antisense primer located in exon 7 while the amplification of the mutant sequence was done with an antisense primer in the PGK-Neo cassette.
T21329	2070-2073	DT	denotes	The
T21330	2074-2082	NN	denotes	reaction
T21331	2114-2118	VBD	denotes	used
T21332	2083-2085	TO	denotes	to
T21333	2086-2092	VB	denotes	detect
T21334	2093-2096	DT	denotes	the
T21335	2107-2113	NN	denotes	allele
T21336	2097-2101	JJ	denotes	wild
T21337	2102-2106	NN	denotes	type
T21338	2101-2102	HYPH	denotes	-
T21339	2119-2121	DT	denotes	an
T21340	2132-2138	NN	denotes	primer
T21341	2122-2131	NN	denotes	antisense
T21342	2139-2146	VBN	denotes	located
T21343	2147-2149	IN	denotes	in
T21344	2150-2154	NN	denotes	exon
T21345	2155-2156	CD	denotes	7
T21346	2157-2162	IN	denotes	while
T21347	2208-2212	VBN	denotes	done
T21348	2163-2166	DT	denotes	the
T21349	2167-2180	NN	denotes	amplification
T21350	2181-2183	IN	denotes	of
T21351	2184-2187	DT	denotes	the
T21352	2195-2203	NN	denotes	sequence
T21353	2188-2194	NN	denotes	mutant
T21354	2204-2207	VBD	denotes	was
T21355	2213-2217	IN	denotes	with
T21356	2218-2220	DT	denotes	an
T21357	2231-2237	NN	denotes	primer
T21358	2221-2230	NN	denotes	antisense
T21359	2238-2240	IN	denotes	in
T21360	2241-2244	DT	denotes	the
T21361	2253-2261	NN	denotes	cassette
T21362	2245-2248	NN	denotes	PGK
T21363	2249-2252	NN	denotes	Neo
T21364	2248-2249	HYPH	denotes	-
T21365	2261-2262	.	denotes	.
T21366	2262-2412	sentence	denotes	The results confirm the presence of the wild-type allele in all cells, whereas the mutant allele signal was observed only in the two targeted clones.
T21367	2263-2266	DT	denotes	The
T21368	2267-2274	NNS	denotes	results
T21369	2275-2282	VBP	denotes	confirm
T21370	2283-2286	DT	denotes	the
T21371	2287-2295	NN	denotes	presence
T21372	2296-2298	IN	denotes	of
T21373	2299-2302	DT	denotes	the
T21374	2313-2319	NN	denotes	allele
T21375	2303-2307	JJ	denotes	wild
T21376	2308-2312	NN	denotes	type
T21377	2307-2308	HYPH	denotes	-
T21378	2320-2322	IN	denotes	in
T21379	2323-2326	DT	denotes	all
T21380	2327-2332	NNS	denotes	cells
T21381	2332-2334	,	denotes	,
T21382	2334-2341	IN	denotes	whereas
T21383	2371-2379	VBN	denotes	observed
T21384	2342-2345	DT	denotes	the
T21385	2360-2366	NN	denotes	signal
T21386	2346-2352	NN	denotes	mutant
T21387	2353-2359	NN	denotes	allele
T21388	2367-2370	VBD	denotes	was
T21389	2380-2384	RB	denotes	only
T21390	2385-2387	IN	denotes	in
T21391	2388-2391	DT	denotes	the
T21392	2405-2411	NNS	denotes	clones
T21393	2392-2395	CD	denotes	two
T21394	2396-2404	VBN	denotes	targeted
T21395	2411-2412	.	denotes	.
T21396	2412-2453	sentence	denotes	(M) denotes the molecular weight marker.
T21397	2413-2414	-LRB-	denotes	(
T21398	2417-2424	VBZ	denotes	denotes
T21399	2414-2415	NN	denotes	M
T21400	2415-2416	-RRB-	denotes	)
T21401	2425-2428	DT	denotes	the
T21402	2446-2452	NN	denotes	marker
T21403	2429-2438	JJ	denotes	molecular
T21404	2439-2445	NN	denotes	weight
T21405	2452-2453	.	denotes	.
T22898	7096-7137	sentence	denotes	(M) denotes the molecular weight marker.
T22897	7095-7096	.	denotes	.
T22896	7083-7084	HYPH	denotes	-
T22895	7084-7088	NN	denotes	type
T22936	4283-4291	NN	denotes	Genotype
T22937	4292-4304	NN	denotes	distribution
T22938	4305-4307	IN	denotes	of
T22939	4308-4317	NN	denotes	offspring
T22940	4318-4325	VBN	denotes	derived
T22941	4326-4330	IN	denotes	from
T22942	4331-4336	NN	denotes	Capn2
T22943	4348-4352	NNS	denotes	mice
T22944	4337-4347	JJ	denotes	transgenic
T22945	4352-4353	.	denotes	.
T6986	4194-4198	VB	denotes	have
T6987	4207-4208	DT	denotes	a
T6988	4215-4221	NN	denotes	number
T6989	4209-4214	JJ	denotes	small
T6990	4245-4252	VB	denotes	survive
T6991	4222-4224	IN	denotes	of
T6992	4225-4230	NN	denotes	Capn2
T6993	4234-4241	NNS	denotes	embryos
T6994	4230-4231	SYM	denotes	-
T6995	4231-4232	HYPH	denotes	/
T6996	4232-4233	SYM	denotes	-
T6997	4242-4244	TO	denotes	to
T6998	4253-4255	IN	denotes	to
T6999	4256-4259	DT	denotes	the
T7000	4267-4272	NN	denotes	stage
T7001	4260-4266	NN	denotes	morula
T7002	4266-4267	HYPH	denotes	-
T7003	4272-4273	.	denotes	.
T7016	7199-7215	JJ	denotes	pre-implantation
T7015	7195-7198	CC	denotes	and
T7014	7193-7195	,	denotes	,
T7013	7216-7223	NNS	denotes	embryos
T7012	7176-7193	JJ	denotes	post-implantation
T7011	7174-7176	,	denotes	,
T7010	7165-7174	NNS	denotes	weanlings
T7009	7161-7164	IN	denotes	for
T7008	7153-7160	VBZ	denotes	results
T7007	7228-7233	VBN	denotes	shown
T7006	7142-7152	NN	denotes	genotyping
T7005	4274-7141	DT	denotes	Table 2 Genotype distribution of offspring derived from Capn2 transgenic mice. Cross (N) Age Genotype +/+ +/- -/- ND* (+/-)♀ × (+/-)♂ 199 3 weeks 23 176 0 0 (+/+)♀ × (+/-)♂ 122 3 weeks 50 72 0 0 (+/-)♀ × (+/+)♂ 103 3 weeks 28 75 0 0 (+/-)♀ × (+/-)♂ 9 E18.5 0 7 0 2 (+/-)♀ × (+/-)♂ 5 E17.5 0 5 0 0 (+/-)♀ × (+/-)♂ 9 E14.5 2 7 0 0 (+/-)♀ × (+/-)♂ 10 E11.5 3 7 0 0 (+/-)♀ × (+/-)♂ 7 E10.5 2 5 0 0 (+/-)♀ × (+/-)♂ 46 E3.5 7 39 0 0 (+/-)♀ × (+/-)♂ 48 E2.5 8 34 2 4 * ND, not determined Figure 4 Nested PCR strategy for genotyping of pre-implantation embryos. Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos. Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector. In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control. The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific. The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions. Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm. A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele. Figure 5 Genotyping of pre-implantation embryos. A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4. Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs. Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K. In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles. The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control. (A) A representative example of the genotyping of blastocyst stage embryos. Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal. (B) An example of the genotyping of 8-cell embryos is shown. Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal. (M) denotes the molecular weight marker. The
T7004	4273-7245	sentence	denotes	Table 2 Genotype distribution of offspring derived from Capn2 transgenic mice. Cross (N) Age Genotype +/+ +/- -/- ND* (+/-)♀ × (+/-)♂ 199 3 weeks 23 176 0 0 (+/+)♀ × (+/-)♂ 122 3 weeks 50 72 0 0 (+/-)♀ × (+/+)♂ 103 3 weeks 28 75 0 0 (+/-)♀ × (+/-)♂ 9 E18.5 0 7 0 2 (+/-)♀ × (+/-)♂ 5 E17.5 0 5 0 0 (+/-)♀ × (+/-)♂ 9 E14.5 2 7 0 0 (+/-)♀ × (+/-)♂ 10 E11.5 3 7 0 0 (+/-)♀ × (+/-)♂ 7 E10.5 2 5 0 0 (+/-)♀ × (+/-)♂ 46 E3.5 7 39 0 0 (+/-)♀ × (+/-)♂ 48 E2.5 8 34 2 4 * ND, not determined Figure 4 Nested PCR strategy for genotyping of pre-implantation embryos. Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos. Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector. In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control. The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific. The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions. Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm. A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele. Figure 5 Genotyping of pre-implantation embryos. A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4. Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs. Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K. In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles. The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control. (A) A representative example of the genotyping of blastocyst stage embryos. Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal. (B) An example of the genotyping of 8-cell embryos is shown. Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal. (M) denotes the molecular weight marker. The genotyping results for weanlings, post-implantation, and pre-implantation embryos are shown in Table 2.
R1307	T4833	T4832	cc	and,Isolation
R1308	T4834	T4832	conj	characterization,Isolation
R1309	T4835	T4832	prep	of,Isolation
R1310	T4836	T4837	npadvmod	Capn2,targeted
R1311	T4837	T4838	amod	targeted,clones
R1312	T4838	T4835	pobj	clones,of
R1313	T4839	T4840	compound	ES,cell
R1314	T4840	T4838	compound	cell,clones
R1315	T4842	T4843	nummod	Two,lines
R1316	T4843	T4851	nsubjpass	lines,isolated
R1317	T4844	T4843	amod	independent,lines
R1318	T4845	T4843	nmod	Capn2,lines
R1319	T4846	T4845	punct	+,Capn2
R1320	T4847	T4845	punct	/,Capn2
R1321	T4848	T4845	punct	-,Capn2
R1322	T4849	T4843	compound	ES,lines
R1323	T4850	T4843	compound	cell,lines
R1324	T4852	T4843	punct	", ",lines
R1325	T4853	T4843	acl	designated,lines
R1326	T4854	T4853	oprd	ES27,designated
R1327	T4855	T4854	cc	and,ES27
R1328	T4856	T4854	conj	ES36,ES27
R1329	T4857	T4851	punct	", ",isolated
R1330	T4858	T4851	auxpass	were,isolated
R1331	T4859	T4851	prep	from,isolated
R1332	T4860	T4861	det	a,screen
R1333	T4861	T4859	pobj	screen,from
R1334	T4862	T4861	prep	of,screen
R1335	T4863	T4864	nummod	305,clones
R1336	T4864	T4862	pobj	clones,of
R1337	T4865	T4866	npadvmod	drug,resistant
R1338	T4866	T4864	amod	resistant,clones
R1339	T4867	T4866	punct	-,resistant
R1340	T4868	T4851	punct	.,isolated
R1341	T4870	T4871	amod	Correct,targeting
R1342	T4871	T4872	nsubjpass	targeting,established
R1343	T4873	T4871	prep	of,targeting
R1344	T4874	T4875	det	the,locus
R1345	T4875	T4873	pobj	locus,of
R1346	T4876	T4875	compound	Capn2,locus
R1347	T4877	T4872	auxpass	was,established
R1348	T4878	T4879	preconj	both,by
R1349	T4879	T4872	agent	by,established
R1350	T4880	T4881	compound	Southern,blot
R1351	T4881	T4882	compound	blot,hybridization
R1352	T4882	T4879	pobj	hybridization,by
R1353	T4883	T4882	cc	and,hybridization
R1354	T4884	T4885	compound	PCR,analysis
R1355	T4885	T4882	conj	analysis,hybridization
R1356	T4886	T4872	punct	.,established
R1357	T4888	T4889	det	A,probe
R1358	T4889	T4890	nsubj	probe,hybridized
R1359	T4891	T4889	acl	located,probe
R1360	T4892	T4891	advmod	outside,located
R1361	T4893	T4894	det	the,arm
R1362	T4894	T4892	npadvmod	arm,outside
R1363	T4895	T4894	amod	short,arm
R1364	T4896	T4897	punct	(,upstream
R1365	T4897	T4895	parataxis	upstream,short
R1366	T4898	T4897	punct	),upstream
R1367	T4899	T4894	prep	of,arm
R1368	T4900	T4899	pobj	homology,of
R1369	T4901	T4890	prep	to,hybridized
R1370	T4902	T4903	det	a,fragment
R1371	T4903	T4901	pobj	fragment,to
R1372	T4904	T4905	nummod	3.5,kb
R1373	T4905	T4903	compound	kb,fragment
R1374	T4906	T4905	punct	-,kb
R1375	T4907	T4903	compound	BamHI,fragment
R1376	T4908	T4903	prep	of,fragment
R1377	T4909	T4910	det	the,allele
R1378	T4910	T4908	pobj	allele,of
R1379	T4911	T4912	amod	wild,type
R1380	T4912	T4910	compound	type,allele
R1381	T4913	T4912	punct	-,type
R1382	T4914	T4903	cc	and,fragment
R1383	T4915	T4916	nummod	5.3,kb
R1384	T4916	T4918	compound	kb,fragment
R1385	T4917	T4916	punct	-,kb
R1386	T4918	T4903	conj	fragment,fragment
R1387	T4919	T4918	compound	BamHI,fragment
R1388	T4920	T4918	prep	of,fragment
R1389	T4921	T4922	det	the,allele
R1390	T4922	T4920	pobj	allele,of
R1391	T4923	T4922	compound	mutant,allele
R1392	T4924	T4925	mark	as,predicted
R1393	T4925	T4890	advcl	predicted,hybridized
R1394	T4926	T4925	prep	from,predicted
R1395	T4927	T4928	amod	genomic,maps
R1396	T4928	T4926	pobj	maps,from
R1397	T4929	T4930	punct	(,2A
R1398	T4930	T4925	parataxis	2A,predicted
R1399	T4931	T4930	compound	Figure,2A
R1400	T4932	T4930	punct	),2A
R1401	T4933	T4890	punct	.,hybridized
R1402	T4935	T4936	det	The,probe
R1403	T4936	T4938	nsubj	probe,detected
R1404	T4937	T4936	amod	same,probe
R1405	T4939	T4938	advmod	also,detected
R1406	T4940	T4941	det	the,fragments
R1407	T4941	T4938	dobj	fragments,detected
R1408	T4942	T4941	amod	expected,fragments
R1409	T4943	T4944	nummod	7.2,kb
R1410	T4944	T4941	nmod	kb,fragments
R1411	T4945	T4944	punct	-,kb
R1412	T4946	T4947	amod	wild,type
R1413	T4947	T4944	appos	type,kb
R1414	T4948	T4947	punct	-,type
R1415	T4949	T4944	cc	and,kb
R1416	T4950	T4951	nummod	6.4,kb
R1417	T4951	T4953	compound	kb,mutant
R1418	T4952	T4951	punct	-,kb
R1419	T4953	T4944	conj	mutant,kb
R1420	T4954	T4941	compound	BglII,fragments
R1421	T4955	T4941	punct	", ",fragments
R1422	T4956	T4957	nummod	4.9,kb
R1423	T4957	T4959	nmod	kb,fragments
R1424	T4958	T4957	punct	-,kb
R1425	T4959	T4941	conj	fragments,fragments
R1426	T4960	T4961	amod	wild,type
R1427	T4961	T4957	appos	type,kb
R1428	T4962	T4961	punct	-,type
R1429	T4963	T4957	cc	and,kb
R1430	T4964	T4965	nummod	5.7,kb
R1431	T4965	T4967	compound	kb,mutant
R1432	T4966	T4965	punct	-,kb
R1433	T4967	T4957	conj	mutant,kb
R1434	T4968	T4959	compound	NcoI,fragments
R1435	T4969	T4959	punct	", ",fragments
R1436	T4970	T4971	advmod	as,as
R1437	T4971	T4959	cc	as,fragments
R1438	T4972	T4971	advmod	well,as
R1439	T4973	T4974	nummod	7.2,kb
R1440	T4974	T4976	nmod	kb,fragments
R1441	T4975	T4974	punct	-,kb
R1442	T4976	T4959	conj	fragments,fragments
R1443	T4977	T4978	amod	wild,type
R1444	T4978	T4974	appos	type,kb
R1445	T4979	T4978	punct	-,type
R1446	T4980	T4974	cc	and,kb
R1447	T4981	T4982	nummod	4.9,kb
R1448	T4982	T4984	compound	kb,mutant
R1449	T4983	T4982	punct	-,kb
R1450	T4984	T4974	conj	mutant,kb
R1451	T4985	T4986	compound	BglII,AgeI
R1452	T4986	T4976	compound	AgeI,fragments
R1453	T4987	T4986	punct	/,AgeI
R1454	T4988	T4989	punct	(,shown
R1455	T4989	T4938	parataxis	shown,detected
R1456	T4990	T4989	neg	not,shown
R1457	T4991	T4989	punct	),shown
R1458	T4992	T4938	punct	.,detected
R1459	T4994	T4995	det	A,probe
R1460	T4995	T4996	nsubj	probe,recognized
R1461	T4997	T4995	acl	derived,probe
R1462	T4998	T4997	prep	from,derived
R1463	T4999	T5000	det	the,cassette
R1464	T5000	T4998	pobj	cassette,from
R1465	T5001	T5002	compound	PGK,Neo
R1466	T5002	T5000	compound	Neo,cassette
R1467	T5003	T5002	punct	-,Neo
R1468	T5004	T5005	advmod	only,fragment
R1469	T5005	T4996	dobj	fragment,recognized
R1470	T5006	T5005	det	the,fragment
R1471	T5007	T5008	nummod	5.3,kb
R1472	T5008	T5005	compound	kb,fragment
R1473	T5009	T5008	punct	-,kb
R1474	T5010	T5005	compound	BamHI,fragment
R1475	T5011	T4996	prep	in,recognized
R1476	T5012	T5013	nmod	Capn2,ES
R1477	T5013	T5017	compound	ES,cells
R1478	T5014	T5013	punct	+,ES
R1479	T5015	T5013	punct	/,ES
R1480	T5016	T5013	punct	-,ES
R1481	T5017	T5011	pobj	cells,in
R1482	T5018	T4996	punct	", ",recognized
R1483	T5019	T4996	advcl	suggesting,recognized
R1484	T5020	T5021	mark	that,integrated
R1485	T5021	T5019	ccomp	integrated,suggesting
R1486	T5022	T5023	det	the,vector
R1487	T5023	T5021	nsubj	vector,integrated
R1488	T5024	T5023	compound	targeting,vector
R1489	T5025	T5021	aux	had,integrated
R1490	T5026	T5027	advmod	solely,at
R1491	T5027	T5021	prep	at,integrated
R1492	T5028	T5029	det	the,locus
R1493	T5029	T5027	pobj	locus,at
R1494	T5030	T5029	compound	Capn2,locus
R1495	T5031	T5032	punct	(,shown
R1496	T5032	T5019	parataxis	shown,suggesting
R1497	T5033	T5032	neg	not,shown
R1498	T5034	T5032	punct	),shown
R1499	T5035	T4996	punct	.,recognized
R1500	T5037	T5038	det	A,method
R1501	T5038	T5041	nsubjpass	method,established
R1502	T5039	T5038	compound	PCR,method
R1503	T5040	T5038	compound	screening,method
R1504	T5042	T5041	auxpass	was,established
R1505	T5043	T5041	advmod	also,established
R1506	T5044	T5045	dep	that,generated
R1507	T5045	T5041	ccomp	generated,established
R1508	T5046	T5047	det	a,product
R1509	T5047	T5045	dobj	product,generated
R1510	T5048	T5049	amod	wild,type
R1511	T5049	T5047	compound	type,product
R1512	T5050	T5049	punct	-,type
R1513	T5051	T5047	prep	of,product
R1514	T5052	T5053	nummod	"2,749",bp
R1515	T5053	T5051	pobj	bp,of
R1516	T5054	T5047	cc	and,product
R1517	T5055	T5056	det	a,product
R1518	T5056	T5047	conj	product,product
R1519	T5057	T5058	nummod	"2,711",bp
R1520	T5058	T5056	compound	bp,product
R1521	T5059	T5056	prep	from,product
R1522	T5060	T5061	det	the,allele
R1523	T5061	T5059	pobj	allele,from
R1524	T5062	T5061	compound	mutant,allele
R1525	T5063	T5041	punct	.,established
R1526	T5065	T5066	det	The,product
R1527	T5066	T5069	nsubj	product,was
R1528	T5067	T5068	nummod	"2,711",bp
R1529	T5068	T5066	compound	bp,product
R1530	T5070	T5069	advmod	only,was
R1531	T5071	T5069	acomp	evident,was
R1532	T5072	T5069	prep	in,was
R1533	T5073	T5074	det	the,lines
R1534	T5074	T5072	pobj	lines,in
R1535	T5075	T5074	nummod	two,lines
R1536	T5076	T5074	amod	targeted,lines
R1537	T5077	T5074	compound	cell,lines
R1538	T5078	T5079	punct	(,2B
R1539	T5079	T5069	parataxis	2B,was
R1540	T5080	T5079	compound	Figure,2B
R1541	T5081	T5079	punct	),2B
R1542	T5082	T5069	punct	.,was
R1543	T6636	T6635	prep	of,Generation
R1544	T6637	T6638	amod	chimeric,mice
R1545	T6638	T6636	pobj	mice,of
R1546	T6639	T6635	cc	and,Generation
R1547	T6640	T6641	compound	germline,transmission
R1548	T6641	T6635	conj	transmission,Generation
R1549	T6642	T6641	prep	of,transmission
R1550	T6643	T6644	det	the,allele
R1551	T6644	T6642	pobj	allele,of
R1552	T6645	T6644	compound	mutant,allele
R1553	T6646	T6644	compound	Capn2,allele
R1554	T6648	T6649	nummod	Eight,mice
R1555	T6649	T6652	nsubjpass	mice,produced
R1556	T6650	T6649	amod	chimeric,mice
R1557	T6651	T6649	amod	male,mice
R1558	T6653	T6652	auxpass	were,produced
R1559	T6654	T6652	prep	in,produced
R1560	T6655	T6656	compound	morula,aggregation
R1561	T6656	T6657	compound	aggregation,experiments
R1562	T6657	T6654	pobj	experiments,in
R1563	T6658	T6652	advcl	using,produced
R1564	T6659	T6660	det	the,line
R1565	T6660	T6658	dobj	line,using
R1566	T6661	T6660	nmod	Capn2,line
R1567	T6662	T6661	punct	+,Capn2
R1568	T6663	T6661	punct	/,Capn2
R1569	T6664	T6661	punct	-,Capn2
R1570	T6665	T6660	compound	ES27,line
R1571	T6666	T6660	compound	cell,line
R1572	T6667	T6652	punct	.,produced
R1573	T6669	T6670	nsubj	Two,transmitted
R1574	T6671	T6669	prep	of,Two
R1575	T6672	T6673	det	these,males
R1576	T6673	T6671	pobj	males,of
R1577	T6674	T6675	det	the,genotype
R1578	T6675	T6670	dobj	genotype,transmitted
R1579	T6676	T6675	nmod	Capn2,genotype
R1580	T6677	T6676	punct	+,Capn2
R1581	T6678	T6676	punct	/,Capn2
R1582	T6679	T6676	punct	-,Capn2
R1583	T6680	T6675	compound	ES27,genotype
R1584	T6681	T6670	prep	through,transmitted
R1585	T6682	T6683	det	the,germline
R1586	T6683	T6681	pobj	germline,through
R1587	T6684	T6670	prep	into,transmitted
R1588	T6685	T6686	det	the,generation
R1589	T6686	T6684	pobj	generation,into
R1590	T6687	T6686	compound	F1,generation
R1591	T6688	T6670	punct	.,transmitted
R1592	T6690	T6691	amod	Heterozygous,animals
R1593	T6691	T6696	nsubj	animals,appeared
R1594	T6692	T6691	nmod	Capn2,animals
R1595	T6693	T6692	punct	+,Capn2
R1596	T6694	T6692	punct	/,Capn2
R1597	T6695	T6692	punct	-,Capn2
R1598	T6697	T6696	oprd	normal,appeared
R1599	T6698	T6696	punct	", ",appeared
R1600	T6699	T6696	prep	with,appeared
R1601	T6700	T6701	det	no,defects
R1602	T6701	T6699	pobj	defects,with
R1603	T6702	T6701	amod	obvious,defects
R1604	T6703	T6701	prep	in,defects
R1605	T6704	T6705	amod	gross,anatomy
R1606	T6705	T6703	pobj	anatomy,in
R1607	T6706	T6705	punct	", ",anatomy
R1608	T6707	T6705	conj	reproduction,anatomy
R1609	T6708	T6707	punct	", ",reproduction
R1610	T6709	T6707	cc	or,reproduction
R1611	T6710	T6711	compound	life,span
R1612	T6711	T6707	conj	span,reproduction
R1613	T6712	T6696	punct	.,appeared
R1614	T6714	T6715	prep	Out,detected
R1615	T6716	T6714	prep	of,Out
R1616	T6717	T6718	nummod	199,weanlings
R1617	T6718	T6716	pobj	weanlings,of
R1618	T6719	T6718	prep	from,weanlings
R1619	T6720	T6721	amod	heterozygous,intercrosses
R1620	T6721	T6719	pobj	intercrosses,from
R1621	T6722	T6715	punct	", ",detected
R1622	T6723	T6724	det	no,progeny
R1623	T6724	T6715	nsubjpass	progeny,detected
R1624	T6725	T6724	nmod	Capn2,progeny
R1625	T6726	T6725	punct	-,Capn2
R1626	T6727	T6725	punct	/,Capn2
R1627	T6728	T6725	punct	-,Capn2
R1628	T6729	T6715	auxpass	were,detected
R1629	T6730	T6731	punct	(,Table
R1630	T6731	T6715	parataxis	Table,detected
R1631	T6732	T6731	nummod	2,Table
R1632	T6733	T6731	punct	),Table
R1633	T6734	T6715	punct	.,detected
R1634	T6736	T6737	nsubj	We,observe
R1635	T6738	T6737	aux	did,observe
R1636	T6739	T6737	neg	not,observe
R1637	T6740	T6741	amod	high,rates
R1638	T6741	T6737	dobj	rates,observe
R1639	T6742	T6741	prep	of,rates
R1640	T6743	T6744	amod	perinatal,death
R1641	T6744	T6742	pobj	death,of
R1642	T6745	T6737	punct	", ",observe
R1643	T6746	T6737	cc	and,observe
R1644	T6747	T6748	det	no,stillborns
R1645	T6748	T6753	nsubjpass	stillborns,observed
R1646	T6749	T6748	nmod	Capn2,stillborns
R1647	T6750	T6749	punct	-,Capn2
R1648	T6751	T6749	punct	/,Capn2
R1649	T6752	T6749	punct	-,Capn2
R1650	T6753	T6737	conj	observed,observe
R1651	T6754	T6753	auxpass	were,observed
R1652	T6755	T6753	punct	.,observed
R1653	T6757	T6758	nsubj	This,suggested
R1654	T6759	T6760	mark	that,perished
R1655	T6760	T6758	ccomp	perished,suggested
R1656	T6761	T6762	nmod	Capn2,animals
R1657	T6762	T6760	nsubj	animals,perished
R1658	T6763	T6761	punct	-,Capn2
R1659	T6764	T6761	punct	/,Capn2
R1660	T6765	T6761	punct	-,Capn2
R1661	T6766	T6760	prep	at,perished
R1662	T6767	T6768	det	some,stage
R1663	T6768	T6766	pobj	stage,at
R1664	T6769	T6768	prep	during,stage
R1665	T6770	T6771	amod	embryonic,development
R1666	T6771	T6769	pobj	development,during
R1667	T6772	T6758	punct	.,suggested
R1668	T6774	T6775	prep	In,harvested
R1669	T6776	T6777	det	an,attempt
R1670	T6777	T6774	pobj	attempt,In
R1671	T6778	T6779	aux	to,determine
R1672	T6779	T6777	acl	determine,attempt
R1673	T6780	T6781	mark	if,occurred
R1674	T6781	T6779	ccomp	occurred,determine
R1675	T6782	T6783	amod	embryonic,death
R1676	T6783	T6781	nsubj	death,occurred
R1677	T6784	T6781	prep	at,occurred
R1678	T6785	T6786	det	a,stage
R1679	T6786	T6784	pobj	stage,at
R1680	T6787	T6786	amod	post-implantation,stage
R1681	T6788	T6775	punct	", ",harvested
R1682	T6789	T6775	nsubjpass	embryos,harvested
R1683	T6790	T6775	auxpass	were,harvested
R1684	T6791	T6775	prep	for,harvested
R1685	T6792	T6791	pobj	genotyping,for
R1686	T6793	T6775	prep	at,harvested
R1687	T6794	T6795	amod	different,times
R1688	T6795	T6793	pobj	times,at
R1689	T6796	T6795	prep	between,times
R1690	T6797	T6796	pobj	E10.5,between
R1691	T6798	T6797	cc	and,E10.5
R1692	T6799	T6797	conj	E18.5,E10.5
R1693	T6800	T6775	punct	.,harvested
R1694	T6802	T6803	det	No,embryos
R1695	T6803	T6808	nsubjpass	embryos,observed
R1696	T6804	T6803	nmod	Capn2,embryos
R1697	T6805	T6804	punct	-,Capn2
R1698	T6806	T6804	punct	/,Capn2
R1699	T6807	T6804	punct	-,Capn2
R1700	T6809	T6808	auxpass	were,observed
R1701	T6810	T6808	cc	and,observed
R1702	T6811	T6812	det	no,signs
R1703	T6812	T6813	nsubjpass	signs,detected
R1704	T6813	T6808	conj	detected,observed
R1705	T6814	T6812	prep	of,signs
R1706	T6815	T6816	compound	embryo,resorption
R1707	T6816	T6814	pobj	resorption,of
R1708	T6817	T6813	auxpass	were,detected
R1709	T6818	T6819	punct	(,Table
R1710	T6819	T6813	parataxis	Table,detected
R1711	T6820	T6819	nummod	2,Table
R1712	T6821	T6819	punct	),Table
R1713	T6822	T6813	punct	.,detected
R1714	T6824	T6825	nsubj	This,indicated
R1715	T6826	T6827	mark	that,dying
R1716	T6827	T6825	ccomp	dying,indicated
R1717	T6828	T6829	det	the,embryos
R1718	T6829	T6827	nsubj	embryos,dying
R1719	T6830	T6829	nmod	Capn2,embryos
R1720	T6831	T6830	punct	-,Capn2
R1721	T6832	T6830	punct	/,Capn2
R1722	T6833	T6830	punct	-,Capn2
R1723	T6834	T6827	aux	might,dying
R1724	T6835	T6827	aux	be,dying
R1725	T6836	T6837	amod	prior,to
R1726	T6837	T6827	prep	to,dying
R1727	T6838	T6837	pobj	implantation,to
R1728	T6839	T6825	punct	.,indicated
R1729	T6841	T6842	nsubjpass	Embryos,flushed
R1730	T6843	T6842	auxpass	were,flushed
R1731	T6844	T6842	advmod	then,flushed
R1732	T6845	T6842	prep	from,flushed
R1733	T6846	T6847	det	the,oviducts
R1734	T6847	T6845	pobj	oviducts,from
R1735	T6848	T6847	prep	of,oviducts
R1736	T6849	T6850	amod	pregnant,females
R1737	T6850	T6848	pobj	females,of
R1738	T6851	T6842	prep	at,flushed
R1739	T6852	T6851	pobj	E2.5,at
R1740	T6853	T6852	cc	or,E2.5
R1741	T6854	T6852	conj	E3.5,E2.5
R1742	T6855	T6842	punct	", ",flushed
R1743	T6856	T6842	cc	and,flushed
R1744	T6857	T6842	conj	genotyped,flushed
R1745	T6858	T6857	prep	by,genotyped
R1746	T6859	T6858	pobj	means,by
R1747	T6860	T6859	prep	of,means
R1748	T6861	T6862	det	a,strategy
R1749	T6862	T6860	pobj	strategy,of
R1750	T6863	T6862	amod	nested,strategy
R1751	T6864	T6862	compound	PCR,strategy
R1752	T6865	T6866	punct	(,Figure
R1753	T6866	T6857	parataxis	Figure,genotyped
R1754	T6867	T6866	nummod	4,Figure
R1755	T6868	T6866	punct	),Figure
R1756	T6869	T6842	punct	.,flushed
R1757	T6871	T6872	nsubj	Two,were
R1758	T6873	T6871	prep	of,Two
R1759	T6874	T6875	nummod	90,embryos
R1760	T6875	T6873	pobj	embryos,of
R1761	T6876	T6877	advmod	successfully,genotyped
R1762	T6877	T6875	amod	genotyped,embryos
R1763	T6878	T6875	amod	pre-implantation,embryos
R1764	T6879	T6872	attr	Capn2,were
R1765	T6880	T6879	punct	-,Capn2
R1766	T6881	T6879	punct	/,Capn2
R1767	T6882	T6879	punct	-,Capn2
R1768	T6883	T6872	punct	", ",were
R1769	T6884	T6885	punct	(,Figure
R1770	T6885	T6872	parataxis	Figure,were
R1771	T6886	T6885	dep	Table,Figure
R1772	T6887	T6886	nummod	2,Table
R1773	T6888	T6885	punct	;,Figure
R1774	T6889	T6885	nummod	5,Figure
R1775	T6890	T6885	punct	),Figure
R1776	T6891	T6872	punct	.,were
R1777	T6893	T6894	nsubjpass	Both,isolated
R1778	T6895	T6893	prep	of,Both
R1779	T6896	T6897	det	these,embryos
R1780	T6897	T6895	pobj	embryos,of
R1781	T6898	T6897	nmod	Capn2,embryos
R1782	T6899	T6898	punct	-,Capn2
R1783	T6900	T6898	punct	/,Capn2
R1784	T6901	T6898	punct	-,Capn2
R1785	T6902	T6894	auxpass	were,isolated
R1786	T6903	T6894	prep	at,isolated
R1787	T6904	T6905	det	the,stage
R1788	T6905	T6903	pobj	stage,at
R1789	T6906	T6907	nummod	8,cell
R1790	T6907	T6905	compound	cell,stage
R1791	T6908	T6907	punct	-,cell
R1792	T6909	T6894	cc	and,isolated
R1793	T6910	T6911	aux	did,display
R1794	T6911	T6894	conj	display,isolated
R1795	T6912	T6911	neg	not,display
R1796	T6913	T6914	det	any,defects
R1797	T6914	T6911	dobj	defects,display
R1798	T6915	T6914	amod	obvious,defects
R1799	T6916	T6914	amod	morphological,defects
R1800	T6917	T6894	punct	.,isolated
R1802	T6921	T6919	prep	of,None
R1803	T6922	T6923	det	the,embryos
R1804	T6923	T6921	pobj	embryos,of
R1805	T6924	T6923	nummod	46,embryos
R1806	T6925	T6926	advmod	successfully,genotyped
R1807	T6926	T6923	amod	genotyped,embryos
R1808	T6927	T6928	npadvmod	blastocyst,staged
R1809	T6928	T6923	amod	staged,embryos
R1810	T6929	T6928	punct	-,staged
R1811	T6930	T6920	attr	Capn2,were
R1812	T6931	T6930	punct	-,Capn2
R1813	T6932	T6930	punct	/,Capn2
R1814	T6933	T6930	punct	-,Capn2
R1815	T6934	T6920	punct	.,were
R1816	T6936	T6937	det	The,scarcity
R1817	T6937	T6938	nsubj	scarcity,suggested
R1818	T6939	T6937	prep	of,scarcity
R1819	T6940	T6941	npadvmod	Capn2,deficient
R1820	T6941	T6943	amod	deficient,embryos
R1821	T6942	T6941	punct	-,deficient
R1822	T6943	T6939	pobj	embryos,of
R1823	T6944	T6943	acl	surviving,embryos
R1824	T6945	T6944	prep	to,surviving
R1825	T6946	T6947	det	the,stage
R1826	T6947	T6945	pobj	stage,to
R1827	T6948	T6949	nummod	8,cell
R1828	T6949	T6947	compound	cell,stage
R1829	T6950	T6949	punct	-,cell
R1830	T6951	T6952	mark	that,compromise
R1831	T6952	T6938	ccomp	compromise,suggested
R1832	T6953	T6954	det	the,loss
R1833	T6954	T6952	nsubj	loss,compromise
R1834	T6955	T6954	prep	of,loss
R1835	T6956	T6957	compound	m,calpain
R1836	T6957	T6959	compound	calpain,activity
R1837	T6958	T6957	punct	-,calpain
R1838	T6959	T6955	pobj	activity,of
R1839	T6960	T6952	aux	must,compromise
R1840	T6961	T6952	advmod	fatally,compromise
R1841	T6962	T6963	det	the,viability
R1842	T6963	T6952	dobj	viability,compromise
R1843	T6964	T6963	prep	of,viability
R1844	T6965	T6966	amod	early,embryos
R1845	T6966	T6964	pobj	embryos,of
R1846	T6967	T6938	punct	.,suggested
R1847	T6969	T6970	advmod	Furthermore,is
R1848	T6971	T6970	punct	", ",is
R1849	T6972	T6970	nsubj	it,is
R1850	T6973	T6970	acomp	possible,is
R1851	T6974	T6975	mark	that,allowed
R1852	T6975	T6970	ccomp	allowed,is
R1853	T6976	T6975	nsubj	persistence,allowed
R1854	T6977	T6976	prep	of,persistence
R1855	T6978	T6979	det	some,transcript
R1856	T6979	T6977	pobj	transcript,of
R1857	T6980	T6981	advmod	maternally,derived
R1858	T6981	T6979	amod	derived,transcript
R1859	T6982	T6979	compound	mRNA,transcript
R1860	T6983	T6979	cc	or,transcript
R1861	T6984	T6979	conj	protein,transcript
R1862	T6985	T6975	aux	might,allowed
R1863	T6986	T6975	aux	have,allowed
R1864	T6987	T6988	det	a,number
R1865	T6988	T6990	nsubj	number,survive
R1866	T6989	T6988	amod	small,number
R1867	T6990	T6975	ccomp	survive,allowed
R1868	T6991	T6988	prep	of,number
R1869	T6992	T6993	nmod	Capn2,embryos
R1870	T6993	T6991	pobj	embryos,of
R1871	T6994	T6992	punct	-,Capn2
R1872	T6995	T6992	punct	/,Capn2
R1873	T6996	T6992	punct	-,Capn2
R1874	T6997	T6990	aux	to,survive
R1875	T6998	T6990	prep	to,survive
R1876	T6999	T7000	det	the,stage
R1877	T7000	T6998	pobj	stage,to
R1878	T7001	T7000	compound	morula,stage
R1879	T7002	T7000	punct	-,stage
R1880	T7003	T6970	punct	.,is
R1892	T7017	T7007	auxpass	are,shown
R1893	T7018	T7007	prep	in,shown
R1894	T7019	T7018	pobj	Table,in
R1895	T7020	T7019	nummod	2,Table
R1896	T7021	T7007	punct	.,shown
R1897	T7023	T7024	advmod	Curiously,was
R1898	T7025	T7024	punct	", ",was
R1899	T7026	T7027	det	the,ratio
R1900	T7027	T7024	nsubj	ratio,was
R1901	T7028	T7027	prep	of,ratio
R1902	T7029	T7028	pobj	Capn2,of
R1903	T7030	T7029	punct	+,Capn2
R1904	T7031	T7029	punct	/,Capn2
R1905	T7032	T7029	punct	+,Capn2
R1906	T7033	T7027	prep	to,ratio
R1907	T7034	T7035	nmod	Capn2,animals
R1908	T7035	T7033	pobj	animals,to
R1909	T7036	T7034	punct	+,Capn2
R1910	T7037	T7034	punct	/,Capn2
R1911	T7038	T7034	punct	-,Capn2
R1912	T7039	T7027	prep	from,ratio
R1913	T7040	T7041	amod	inbred,intercrosses
R1914	T7041	T7039	pobj	intercrosses,from
R1915	T7042	T7041	amod	heterozygous,intercrosses
R1916	T7043	T7044	advmod	substantially,less
R1917	T7044	T7024	acomp	less,was
R1918	T7045	T7044	prep	than,less
R1919	T7046	T7047	det	the,ratio
R1920	T7047	T7045	pobj	ratio,than
R1921	T7048	T7047	amod	predicted,ratio
R1922	T7049	T7047	nummod	1,ratio
R1923	T7050	T7051	punct	:,2
R1924	T7051	T7049	prep	2,1
R1925	T7052	T7047	amod	Mendelian,ratio
R1926	T7053	T7024	punct	.,was
R1927	T7055	T7056	prep	In,observed
R1928	T7057	T7058	det	a,group
R1929	T7058	T7055	pobj	group,In
R1930	T7059	T7058	prep	of,group
R1931	T7060	T7061	nummod	199,animals
R1932	T7061	T7059	pobj	animals,of
R1933	T7062	T7061	acl	derived,animals
R1934	T7063	T7062	prep	from,derived
R1935	T7064	T7065	compound	heterozygote,breeding
R1936	T7065	T7063	pobj	breeding,from
R1937	T7066	T7067	punct	(,litters
R1938	T7067	T7065	parataxis	litters,breeding
R1939	T7068	T7067	nummod	33,litters
R1940	T7069	T7067	punct	),litters
R1941	T7070	T7056	punct	", ",observed
R1942	T7071	T7072	nummod	23,Capn2
R1943	T7072	T7056	nsubjpass	Capn2,observed
R1944	T7073	T7072	punct	+,Capn2
R1945	T7074	T7072	punct	/,Capn2
R1946	T7075	T7072	punct	+,Capn2
R1947	T7076	T7077	punct	(,%
R1948	T7077	T7072	parataxis	%,Capn2
R1949	T7078	T7077	nummod	11.6,%
R1950	T7079	T7077	punct	),%
R1951	T7080	T7072	cc	and,Capn2
R1952	T7081	T7082	nummod	176,Capn2
R1953	T7082	T7087	nmod	Capn2,animals
R1954	T7083	T7084	punct	(,%
R1955	T7084	T7082	parataxis	%,Capn2
R1956	T7085	T7084	nummod	88.4,%
R1957	T7086	T7084	punct	),%
R1958	T7087	T7072	conj	animals,Capn2
R1959	T7088	T7082	punct	+,Capn2
R1960	T7089	T7082	punct	/,Capn2
R1961	T7090	T7082	punct	-,Capn2
R1962	T7091	T7056	auxpass	were,observed
R1963	T7092	T7056	punct	.,observed
R1964	T7094	T7095	det	The,ratio
R1965	T7095	T7096	nsubj	ratio,was
R1966	T7097	T7095	prep	of,ratio
R1967	T7098	T7097	pobj	Capn2,of
R1968	T7099	T7098	punct	+,Capn2
R1969	T7100	T7098	punct	/,Capn2
R1970	T7101	T7098	punct	+,Capn2
R1971	T7102	T7095	prep	to,ratio
R1972	T7103	T7104	nmod	Capn2,animals
R1973	T7104	T7102	pobj	animals,to
R1974	T7105	T7103	punct	+,Capn2
R1975	T7106	T7103	punct	/,Capn2
R1976	T7107	T7103	punct	-,Capn2
R1977	T7108	T7095	prep	among,ratio
R1978	T7109	T7108	pobj	males,among
R1979	T7110	T7111	punct	(,%
R1980	T7111	T7109	parataxis	%,males
R1981	T7112	T7111	nummod	14,%
R1982	T7113	T7111	prep	to,%
R1983	T7114	T7115	nummod	82,%
R1984	T7115	T7113	pobj	%,to
R1985	T7116	T7111	punct	),%
R1986	T7117	T7109	cc	or,males
R1987	T7118	T7109	conj	females,males
R1988	T7202	T7183	advcl	was,reported
R1989	T7119	T7120	punct	(,%
R1990	T7203	T7204	det	the,skewing
R1991	T7120	T7118	parataxis	%,females
R1992	T7204	T7202	nsubj	skewing,was
R1993	T7121	T7120	nummod	13,%
R1994	T7205	T7204	compound	genotype,skewing
R1995	T7206	T7202	neg	not,was
R1996	T7207	T7202	prep	as,was
R1997	T7122	T7120	prep	to,%
R1998	T7208	T7207	amod	extreme,as
R1999	T7209	T7210	punct	[,32
R2000	T7123	T7124	nummod	90,%
R2001	T7210	T7202	parataxis	32,was
R2002	T7211	T7210	punct	],32
R2003	T7212	T7183	punct	.,reported
R2004	T7124	T7122	pobj	%,to
R2005	T7214	T7215	nsubj	Crosses,resulted
R2006	T7125	T7120	punct	),%
R2007	T7216	T7214	prep	between,Crosses
R2008	T7217	T7218	nmod	Capn2,animals
R2009	T7218	T7216	pobj	animals,between
R2010	T7219	T7217	punct	+,Capn2
R2011	T7220	T7217	punct	/,Capn2
R2012	T7126	T7096	advmod	essentially,was
R2013	T7221	T7217	punct	+,Capn2
R2014	T7222	T7217	cc	and,Capn2
R2015	T7223	T7217	conj	Capn2,Capn2
R2016	T7224	T7223	punct	+,Capn2
R2017	T7127	T7128	advmod	the,same
R2018	T7128	T7096	acomp	same,was
R2019	T7129	T7130	mark	is,was
R2020	T7225	T7223	punct	/,Capn2
R2021	T7130	T7128	advcl	was,same
R2022	T7226	T7223	punct	-,Capn2
R2023	T7227	T7215	advmod	also,resulted
R2024	T7228	T7215	prep	in,resulted
R2025	T7131	T7130	nsubj	it,was
R2026	T7229	T7230	det	a,proportion
R2027	T7230	T7228	pobj	proportion,in
R2028	T7132	T7130	prep	for,was
R2029	T7231	T7230	amod	greater,proportion
R2030	T7232	T7233	mark	than,expected
R2031	T7233	T7231	advcl	expected,greater
R2032	T7133	T7134	det	the,population
R2033	T7234	T7230	prep	of,proportion
R2034	T7235	T7236	nmod	Capn2,animals
R2035	T7134	T7132	pobj	population,for
R2036	T7236	T7234	pobj	animals,of
R2037	T7237	T7235	punct	+,Capn2
R2038	T7238	T7235	punct	/,Capn2
R2039	T7135	T7134	amod	combined,population
R2040	T7239	T7235	punct	-,Capn2
R2041	T7240	T7241	punct	(,Table
R2042	T7241	T7215	parataxis	Table,resulted
R2043	T7136	T7096	punct	", ",was
R2044	T7242	T7241	nummod	2,Table
R2045	T7243	T7241	punct	),Table
R2046	T7244	T7215	punct	.,resulted
R2047	T7137	T7096	cc	and,was
R2048	T7246	T7247	det	An,degree
R2049	T7247	T7250	nsubjpass	degree,seen
R2050	T7138	T7139	expl	there,were
R2051	T7248	T7249	advmod	even,higher
R2052	T7249	T7247	amod	higher,degree
R2053	T7139	T7096	conj	were,was
R2054	T7251	T7247	prep	of,degree
R2055	T7252	T7253	nmod	Capn2,over-representation
R2056	T7140	T7141	det	an,average
R2057	T7253	T7251	pobj	over-representation,of
R2058	T7254	T7252	punct	+,Capn2
R2059	T7255	T7252	punct	/,Capn2
R2060	T7141	T7139	attr	average,were
R2061	T7256	T7252	punct	-,Capn2
R2062	T7257	T7250	auxpass	was,seen
R2063	T7258	T7259	advmod	when,came
R2064	T7259	T7250	advcl	came,seen
R2065	T7260	T7261	det	the,allele
R2066	T7261	T7259	nsubj	allele,came
R2067	T7142	T7141	prep	of,average
R2068	T7262	T7261	compound	mutant,allele
R2069	T7263	T7264	det	the,mother
R2070	T7264	T7259	dobj	mother,came
R2071	T7143	T7144	nummod	six,pups
R2072	T7265	T7266	punct	(,%
R2073	T7266	T7259	parataxis	%,came
R2074	T7144	T7142	pobj	pups,of
R2075	T7267	T7266	nummod	73,%
R2076	T7268	T7266	punct	),%
R2077	T7269	T7250	prep	compared,seen
R2078	T7145	T7144	prep	per,pups
R2079	T7270	T7269	prep	to,compared
R2080	T7271	T7272	advmod	when,came
R2081	T7146	T7145	pobj	litter,per
R2082	T7272	T7270	pcomp	came,to
R2083	T7273	T7272	nsubj	it,came
R2084	T7147	T7141	punct	", ",average
R2085	T7148	T7149	dep	which,is
R2086	T7274	T7272	prep	from,came
R2087	T7149	T7141	relcl	is,average
R2088	T7275	T7276	det	the,father
R2089	T7276	T7274	pobj	father,from
R2090	T7150	T7149	acomp	normal,is
R2091	T7277	T7278	punct	(,%
R2092	T7278	T7272	parataxis	%,came
R2093	T7279	T7278	nummod	59,%
R2094	T7151	T7149	prep	for,is
R2095	T7280	T7278	punct	),%
R2096	T7281	T7250	punct	.,seen
R2097	T7152	T7153	det	this,strain
R2098	T7283	T7284	prep	In,compared
R2099	T7153	T7151	pobj	strain,for
R2100	T7285	T7286	det	these,crosses
R2101	T7286	T7283	pobj	crosses,In
R2102	T7154	T7153	compound	background,strain
R2103	T7287	T7288	det	the,ratios
R2104	T7288	T7284	nsubj	ratios,compared
R2105	T7289	T7288	prep	of,ratios
R2106	T7155	T7139	punct	.,were
R2107	T7290	T7289	pobj	Capn2,of
R2108	T7291	T7290	punct	+,Capn2
R2109	T7157	T7158	det	A,proportion
R2110	T7292	T7290	punct	/,Capn2
R2111	T7293	T7290	punct	+,Capn2
R2112	T7294	T7288	prep	to,ratios
R2113	T7295	T7296	nmod	Capn2,animals
R2114	T7296	T7294	pobj	animals,to
R2115	T7297	T7295	punct	+,Capn2
R2116	T7298	T7295	punct	/,Capn2
R2117	T7158	T7162	nsubj	proportion,was
R2118	T7299	T7295	punct	-,Capn2
R2119	T7300	T7288	prep	among,ratios
R2120	T7301	T7300	pobj	males,among
R2121	T7159	T7158	amod	larger,proportion
R2122	T7302	T7301	cc	or,males
R2123	T7303	T7301	conj	females,males
R2124	T7304	T7284	advmod	well,compared
R2125	T7160	T7161	mark	than,expected
R2126	T7305	T7284	prep	with,compared
R2127	T7306	T7307	det	the,ratio
R2128	T7161	T7159	advcl	expected,larger
R2129	T7163	T7164	nmod	Capn2,animals
R2130	T7164	T7158	npadvmod	animals,proportion
R2131	T7307	T7305	pobj	ratio,with
R2132	T7165	T7163	punct	+,Capn2
R2133	T7308	T7284	prep	in,compared
R2134	T7309	T7310	det	the,populations
R2135	T7310	T7308	pobj	populations,in
R2136	T7166	T7163	punct	/,Capn2
R2137	T7311	T7310	amod	combined,populations
R2138	T7312	T7284	punct	;,compared
R2139	T7167	T7163	punct	-,Capn2
R2140	T7313	T7314	nummod	77,%
R2141	T7314	T7315	nsubj	%,were
R2142	T7315	T7284	conj	were,compared
R2143	T7168	T7162	advmod	also,was
R2144	T7316	T7314	prep	of,%
R2145	T7317	T7316	pobj	males,of
R2146	T7318	T7314	cc	and,%
R2147	T7169	T7162	acomp	evident,was
R2148	T7319	T7320	nummod	69,%
R2149	T7320	T7314	conj	%,%
R2150	T7170	T7162	prep	in,was
R2151	T7321	T7320	prep	of,%
R2152	T7322	T7321	pobj	females,of
R2153	T7323	T7315	attr	Capn2,were
R2154	T7171	T7172	preconj	both,embryos
R2155	T7324	T7323	punct	+,Capn2
R2156	T7325	T7323	punct	/,Capn2
R2157	T7326	T7323	punct	-,Capn2
R2158	T7172	T7170	pobj	embryos,in
R2159	T7327	T7328	advmod	when,came
R2160	T7328	T7315	advcl	came,were
R2161	T7329	T7330	det	the,allele
R2162	T7173	T7172	nmod	post-implantation,embryos
R2163	T7330	T7328	nsubj	allele,came
R2164	T7331	T7330	compound	mutant,allele
R2165	T7332	T7328	prep	from,came
R2166	T7333	T7334	det	the,mother
R2167	T7334	T7332	pobj	mother,from
R2168	T7335	T7315	punct	", ",were
R2169	T7174	T7173	cc	and,post-implantation
R2170	T7336	T7315	cc	and,were
R2171	T7337	T7338	nummod	62,%
R2172	T7338	T7339	nsubj	%,were
R2173	T7175	T7173	conj	pre-implantation,post-implantation
R2174	T7339	T7315	conj	were,were
R2175	T7340	T7338	prep	of,%
R2176	T7341	T7340	pobj	males,of
R2177	T7176	T7177	punct	(,Table
R2178	T7342	T7338	cc	and,%
R2179	T7343	T7344	nummod	55,%
R2180	T7344	T7338	conj	%,%
R2181	T7177	T7162	parataxis	Table,was
R2182	T7345	T7344	prep	of,%
R2183	T7346	T7345	pobj	females,of
R2184	T7347	T7339	attr	Capn2,were
R2185	T7178	T7177	nummod	2,Table
R2186	T7348	T7347	punct	+,Capn2
R2187	T7349	T7347	punct	/,Capn2
R2188	T7350	T7347	punct	-,Capn2
R2189	T7179	T7177	punct	),Table
R2190	T7351	T7352	advmod	when,came
R2191	T7352	T7339	advcl	came,were
R2192	T7180	T7162	punct	.,was
R2193	T7353	T7354	det	the,allele
R2194	T7354	T7352	nsubj	allele,came
R2195	T7355	T7354	compound	mutant,allele
R2196	T7182	T7183	advmod	Interestingly,reported
R2197	T7356	T7352	prep	from,came
R2198	T7184	T7183	punct	", ",reported
R2199	T7357	T7358	det	the,father
R2200	T7358	T7356	pobj	father,from
R2201	T7185	T7186	det	a,over-representation
R2202	T7359	T7339	punct	.,were
R2203	T7186	T7183	nsubjpass	over-representation,reported
R2204	T7187	T7186	amod	similar,over-representation
R2205	T7188	T7186	prep	of,over-representation
R2206	T7189	T7190	amod	heterozygous,animals
R2207	T7190	T7188	pobj	animals,of
R2208	T7191	T7183	auxpass	was,reported
R2209	T7192	T7183	advmod	also,reported
R2210	T7193	T7183	prep	in,reported
R2211	T7194	T7193	pobj	one,in
R2212	T7195	T7194	prep	of,one
R2213	T7196	T7197	det	the,lines
R2214	T7197	T7195	pobj	lines,of
R2215	T7198	T7197	nmod	Capn4,lines
R2216	T7199	T7197	amod	transgenic,lines
R2217	T7200	T7183	punct	", ",reported
R2218	T7201	T7202	mark	though,was
R2221	T7704	T7705	amod	Attempted,generation
R2222	T7706	T7705	prep	of,generation
R2223	T7707	T7708	nmod	Capn2,cells
R2224	T7708	T7706	pobj	cells,of
R2225	T7709	T7707	punct	-,Capn2
R2226	T7710	T7707	punct	/,Capn2
R2227	T7711	T7707	punct	-,Capn2
R2228	T7712	T7708	compound	ES,cells
R2229	T7714	T7715	nmod	Capn2,cells
R2230	T7715	T7720	nsubjpass	cells,subjected
R2231	T7716	T7714	punct	+,Capn2
R2232	T7717	T7714	punct	/,Capn2
R2233	T7718	T7714	punct	-,Capn2
R2234	T7719	T7715	compound	ES,cells
R2235	T7721	T7720	auxpass	were,subjected
R2236	T7722	T7720	prep	to,subjected
R2237	T7723	T7724	amod	clonal,selection
R2238	T7724	T7722	pobj	selection,to
R2239	T7725	T7720	prep	in,subjected
R2240	T7726	T7727	det	the,presence
R2241	T7727	T7725	pobj	presence,in
R2242	T7728	T7727	prep	of,presence
R2243	T7729	T7730	nummod	2,mg
R2244	T7730	T7731	nmod	mg,G418
R2245	T7731	T7728	pobj	G418,of
R2246	T7732	T7733	punct	/,mL
R2247	T7733	T7730	prep	mL,mg
R2248	T7734	T7727	prep	in,presence
R2249	T7735	T7734	pobj	attempts,in
R2250	T7736	T7737	aux	to,generate
R2251	T7737	T7735	acl	generate,attempts
R2252	T7738	T7739	amod	homozygous,cells
R2253	T7739	T7737	dobj	cells,generate
R2254	T7740	T7739	compound	mutant,cells
R2255	T7741	T7737	prep	by,generate
R2256	T7742	T7743	compound	gene,conversion
R2257	T7743	T7741	pobj	conversion,by
R2258	T7744	T7720	punct	.,subjected
R2259	T7746	T7747	det	This,procedure
R2260	T7747	T7748	nsubjpass	procedure,applied
R2261	T7749	T7748	aux	has,applied
R2262	T7750	T7748	auxpass	been,applied
R2263	T7751	T7748	advmod	extensively,applied
R2264	T7752	T7748	prep	to,applied
R2265	T7753	T7754	amod	targeted,cells
R2266	T7754	T7752	pobj	cells,to
R2267	T7755	T7754	compound	ES,cells
R2268	T7756	T7748	cc	and,applied
R2269	T7757	T7758	auxpass	was,achieved
R2270	T7758	T7748	conj	achieved,applied
R2271	T7759	T7758	advmod	readily,achieved
R2272	T7760	T7758	prep	in,achieved
R2273	T7761	T7762	det	the,case
R2274	T7762	T7760	pobj	case,in
R2275	T7763	T7762	prep	of,case
R2276	T7764	T7765	nmod	Capn4,cells
R2277	T7765	T7763	pobj	cells,of
R2278	T7766	T7764	punct	+,Capn4
R2279	T7767	T7764	punct	/,Capn4
R2280	T7768	T7764	punct	-,Capn4
R2281	T7769	T7765	compound	ES,cells
R2282	T7770	T7771	punct	[,31
R2283	T7771	T7758	parataxis	31,achieved
R2284	T7772	T7771	punct	],31
R2285	T7773	T7748	punct	.,applied
R2286	T7775	T7776	prep	In,isolated
R2287	T7777	T7778	det	this,case
R2288	T7778	T7775	pobj	case,In
R2289	T7779	T7776	punct	", ",isolated
R2290	T7780	T7776	advmod	however,isolated
R2291	T7781	T7776	punct	", ",isolated
R2292	T7782	T7783	det	no,cells
R2293	T7783	T7776	nsubjpass	cells,isolated
R2294	T7784	T7783	nmod	Capn2,cells
R2295	T7785	T7784	punct	-,Capn2
R2296	T7786	T7784	punct	/,Capn2
R2297	T7787	T7784	punct	-,Capn2
R2298	T7788	T7783	compound	ES,cells
R2299	T7789	T7776	auxpass	were,isolated
R2300	T7790	T7776	prep	in,isolated
R2301	T7791	T7790	pobj	screens,in
R2302	T7792	T7791	prep	of,screens
R2303	T7793	T7794	nummod	126,clones
R2304	T7794	T7792	pobj	clones,of
R2305	T7795	T7796	npadvmod	drug,resistant
R2306	T7796	T7794	amod	resistant,clones
R2307	T7797	T7796	punct	-,resistant
R2308	T7798	T7776	punct	.,isolated
R2309	T7800	T7801	det	The,inability
R2310	T7801	T7802	nsubj	inability,suggested
R2311	T7803	T7804	aux	to,isolate
R2312	T7804	T7801	acl	isolate,inability
R2313	T7805	T7806	nmod	Capn2,cells
R2314	T7806	T7804	dobj	cells,isolate
R2315	T7807	T7805	punct	-,Capn2
R2316	T7808	T7805	punct	/,Capn2
R2317	T7809	T7805	punct	-,Capn2
R2318	T7810	T7806	compound	ES,cells
R2319	T7811	T7804	punct	", ",isolate
R2320	T7812	T7804	prep	in,isolate
R2321	T7813	T7812	pobj	concert,in
R2322	T7814	T7813	prep	with,concert
R2323	T7815	T7816	det	the,absence
R2324	T7816	T7814	pobj	absence,with
R2325	T7817	T7816	prep	of,absence
R2326	T7818	T7819	nmod	Capn2,embryos
R2327	T7819	T7817	pobj	embryos,of
R2328	T7820	T7818	punct	-,Capn2
R2329	T7821	T7818	punct	/,Capn2
R2330	T7822	T7818	punct	-,Capn2
R2331	T7823	T7816	prep	beyond,absence
R2332	T7824	T7825	det	the,stage
R2333	T7825	T7823	pobj	stage,beyond
R2334	T7826	T7827	nummod	8,cell
R2335	T7827	T7825	compound	cell,stage
R2336	T7828	T7827	punct	-,cell
R2337	T7829	T7802	punct	", ",suggested
R2338	T7830	T7831	mark	that,be
R2339	T7831	T7802	ccomp	be,suggested
R2340	T7832	T7833	compound	m,calpain
R2341	T7833	T7835	compound	calpain,activity
R2342	T7834	T7833	punct	-,calpain
R2343	T7835	T7831	nsubj	activity,be
R2344	T7836	T7831	aux	might,be
R2345	T7837	T7831	acomp	essential,be
R2346	T7838	T7837	prep	for,essential
R2347	T7839	T7840	compound	cell,viability
R2348	T7840	T7838	pobj	viability,for
R2349	T7841	T7840	cc	or,viability
R2350	T7842	T7843	det	the,establishment
R2351	T7843	T7840	conj	establishment,viability
R2352	T7844	T7843	prep	of,establishment
R2353	T7845	T7846	amod	viable,clones
R2354	T7846	T7844	pobj	clones,of
R2355	T7847	T7846	compound	ES,clones
R2356	T7848	T7846	compound	cell,clones
R2357	T7849	T7802	punct	.,suggested
R6232	T21152	T21151	prep	of,Characterization
R6233	T21153	T21154	amod	targeted,lines
R6234	T21154	T21152	pobj	lines,of
R6235	T21155	T21156	compound	ES,cell
R6236	T21156	T21154	compound	cell,lines
R6237	T21157	T21151	punct	.,Characterization
R6238	T21159	T21160	punct	(,A
R6239	T21160	T21161	meta	A,detected
R6240	T21162	T21160	punct	),A
R6241	T21163	T21164	amod	Targeted,disruption
R6242	T21164	T21161	nsubjpass	disruption,detected
R6243	T21165	T21164	prep	of,disruption
R6244	T21166	T21167	det	the,locus
R6245	T21167	T21165	pobj	locus,of
R6246	T21168	T21167	compound	Capn2,locus
R6247	T21169	T21161	auxpass	was,detected
R6248	T21170	T21161	advmod	initially,detected
R6249	T21171	T21161	agent	by,detected
R6250	T21172	T21173	compound	Southern,blotting
R6251	T21173	T21171	pobj	blotting,by
R6252	T21174	T21161	punct	.,detected
R6253	T21176	T21177	nsubjpass	Membranes,blotted
R6254	T21178	T21177	auxpass	were,blotted
R6255	T21179	T21177	prep	with,blotted
R6256	T21180	T21181	npadvmod	BamHI,digested
R6257	T21181	T21183	amod	digested,DNA
R6258	T21182	T21181	punct	-,digested
R6259	T21183	T21179	pobj	DNA,with
R6260	T21184	T21183	amod	genomic,DNA
R6261	T21185	T21183	acl	extracted,DNA
R6262	T21186	T21185	prep	from,extracted
R6263	T21187	T21188	compound	ES,cells
R6264	T21188	T21186	pobj	cells,from
R6265	T21189	T21177	cc	and,blotted
R6266	T21190	T21177	conj	hybridized,blotted
R6267	T21191	T21190	prep	with,hybridized
R6268	T21192	T21193	det	a,fragment
R6269	T21193	T21191	pobj	fragment,with
R6270	T21194	T21195	npadvmod	DIG,labeled
R6271	T21195	T21193	amod	labeled,fragment
R6272	T21196	T21195	punct	-,labeled
R6273	T21197	T21198	nummod	823,bp
R6274	T21198	T21193	compound	bp,fragment
R6275	T21199	T21200	compound	BamHI,HindIII
R6276	T21200	T21193	compound	HindIII,fragment
R6277	T21201	T21200	punct	/,HindIII
R6278	T21202	T21193	acl	located,fragment
R6279	T21203	T21202	advmod	immediately,located
R6280	T21204	T21203	advmod	upstream,immediately
R6281	T21205	T21203	prep	of,immediately
R6282	T21206	T21207	det	the,arm
R6283	T21207	T21205	pobj	arm,of
R6284	T21208	T21207	amod	short,arm
R6285	T21209	T21207	prep	of,arm
R6286	T21210	T21211	det	the,vector
R6287	T21211	T21209	pobj	vector,of
R6288	T21212	T21211	compound	targeting,vector
R6289	T21213	T21214	punct	(,Figure
R6290	T21214	T21190	parataxis	Figure,hybridized
R6291	T21215	T21214	nummod	1,Figure
R6292	T21216	T21214	punct	),Figure
R6293	T21217	T21177	punct	.,blotted
R6294	T21219	T21220	det	A,fragment
R6295	T21220	T21225	nsubj	fragment,was
R6296	T21221	T21222	nummod	3.5,kb
R6297	T21222	T21220	compound	kb,fragment
R6298	T21223	T21222	punct	-,kb
R6299	T21224	T21220	compound	BamHI,fragment
R6300	T21226	T21220	acl	corresponding,fragment
R6301	T21227	T21226	prep	to,corresponding
R6302	T21228	T21229	det	the,allele
R6303	T21229	T21227	pobj	allele,to
R6304	T21230	T21231	amod	wild,type
R6305	T21231	T21229	compound	type,allele
R6306	T21232	T21231	punct	-,type
R6307	T21233	T21225	acomp	present,was
R6308	T21234	T21225	prep	in,was
R6309	T21235	T21236	det	all,cells
R6310	T21236	T21234	pobj	cells,in
R6311	T21237	T21225	punct	", ",was
R6312	T21238	T21239	mark	whereas,detected
R6313	T21239	T21225	advcl	detected,was
R6314	T21240	T21241	det	a,fragment
R6315	T21241	T21239	nsubjpass	fragment,detected
R6316	T21242	T21243	nummod	5.3,kb
R6317	T21243	T21241	compound	kb,fragment
R6318	T21244	T21243	punct	-,kb
R6319	T21245	T21241	prep	from,fragment
R6320	T21246	T21247	det	the,allele
R6321	T21247	T21245	pobj	allele,from
R6322	T21248	T21247	compound	mutant,allele
R6323	T21249	T21239	auxpass	was,detected
R6324	T21250	T21239	prep	in,detected
R6325	T21251	T21252	nummod	two,lines
R6326	T21252	T21250	pobj	lines,in
R6327	T21253	T21252	amod	targeted,lines
R6328	T21254	T21252	compound	cell,lines
R6329	T21255	T21239	punct	", ",detected
R6330	T21256	T21239	dep	designated,detected
R6331	T21257	T21256	oprd	ES27,designated
R6332	T21258	T21257	cc	and,ES27
R6333	T21259	T21257	conj	ES36,ES27
R6334	T21260	T21225	punct	.,was
R6335	T21262	T21263	punct	(,B
R6336	T21263	T21264	meta	B,carried
R6337	T21265	T21263	punct	),B
R6338	T21266	T21267	compound	PCR,genotyping
R6339	T21267	T21264	nsubjpass	genotyping,carried
R6340	T21268	T21264	auxpass	was,carried
R6341	T21269	T21264	prt	out,carried
R6342	T21270	T21264	prep	with,carried
R6343	T21271	T21272	nummod	two,reactions
R6344	T21272	T21270	pobj	reactions,with
R6345	T21273	T21272	amod	separate,reactions
R6346	T21274	T21272	acl	designed,reactions
R6347	T21275	T21276	aux	to,amplify
R6348	T21276	T21274	advcl	amplify,designed
R6349	T21277	T21278	preconj	either,segment
R6350	T21278	T21276	dobj	segment,amplify
R6351	T21279	T21278	det	a,segment
R6352	T21280	T21281	nummod	"2,748",bp
R6353	T21281	T21278	compound	bp,segment
R6354	T21282	T21278	prep	from,segment
R6355	T21283	T21284	det	the,allele
R6356	T21284	T21282	pobj	allele,from
R6357	T21285	T21286	amod	wild,type
R6358	T21286	T21284	compound	type,allele
R6359	T21287	T21286	punct	-,type
R6360	T21288	T21278	cc	or,segment
R6361	T21289	T21290	det	a,segment
R6362	T21290	T21278	conj	segment,segment
R6363	T21291	T21292	nummod	"2,711",bp
R6364	T21292	T21290	compound	bp,segment
R6365	T21293	T21290	prep	from,segment
R6366	T21294	T21295	det	the,allele
R6367	T21295	T21293	pobj	allele,from
R6368	T21296	T21295	compound	mutant,allele
R6369	T21297	T21264	punct	.,carried
R6370	T21299	T21300	det	Both,reactions
R6371	T21300	T21301	nsubj	reactions,used
R6372	T21302	T21303	det	a,primer
R6373	T21303	T21301	dobj	primer,used
R6374	T21304	T21305	amod	common,sense
R6375	T21305	T21303	compound	sense,primer
R6376	T21306	T21303	acl	located,primer
R6377	T21307	T21306	prep	in,located
R6378	T21308	T21307	pobj	intron,in
R6379	T21309	T21308	nummod	4,intron
R6380	T21310	T21306	punct	", ",located
R6381	T21311	T21306	prep	outside,located
R6382	T21312	T21313	det	the,arm
R6383	T21313	T21311	pobj	arm,outside
R6384	T21314	T21313	amod	short,arm
R6385	T21315	T21313	prep	of,arm
R6386	T21316	T21317	det	the,vector
R6387	T21317	T21315	pobj	vector,of
R6388	T21318	T21317	compound	targeting,vector
R6389	T21319	T21313	punct	", ",arm
R6390	T21320	T21313	cc	and,arm
R6391	T21321	T21322	amod	distinct,primers
R6392	T21322	T21313	conj	primers,arm
R6393	T21323	T21324	npadvmod	allele,specific
R6394	T21324	T21322	amod	specific,primers
R6395	T21325	T21324	punct	-,specific
R6396	T21326	T21322	compound	antisense,primers
R6397	T21327	T21301	punct	.,used
R6398	T21329	T21330	det	The,reaction
R6399	T21330	T21331	nsubj	reaction,used
R6400	T21332	T21333	aux	to,detect
R6401	T21333	T21330	advcl	detect,reaction
R6402	T21334	T21335	det	the,allele
R6403	T21335	T21333	dobj	allele,detect
R6404	T21336	T21337	amod	wild,type
R6405	T21337	T21335	compound	type,allele
R6406	T21338	T21337	punct	-,type
R6407	T21339	T21340	det	an,primer
R6408	T21340	T21331	dobj	primer,used
R6409	T21341	T21340	compound	antisense,primer
R6410	T21342	T21340	acl	located,primer
R6411	T21343	T21342	prep	in,located
R6412	T21344	T21343	pobj	exon,in
R6413	T21345	T21344	nummod	7,exon
R6414	T21346	T21347	mark	while,done
R6415	T21347	T21331	advcl	done,used
R6416	T21348	T21349	det	the,amplification
R6417	T21349	T21347	nsubjpass	amplification,done
R6418	T21350	T21349	prep	of,amplification
R6419	T21351	T21352	det	the,sequence
R6420	T21352	T21350	pobj	sequence,of
R6421	T21353	T21352	compound	mutant,sequence
R6422	T21354	T21347	auxpass	was,done
R6423	T21355	T21347	prep	with,done
R6424	T21356	T21357	det	an,primer
R6425	T21357	T21355	pobj	primer,with
R6426	T21358	T21357	compound	antisense,primer
R6427	T21359	T21357	prep	in,primer
R6428	T21360	T21361	det	the,cassette
R6429	T21361	T21359	pobj	cassette,in
R6430	T21362	T21363	compound	PGK,Neo
R6431	T21363	T21361	compound	Neo,cassette
R6432	T21364	T21363	punct	-,Neo
R6433	T21365	T21331	punct	.,used
R6434	T21367	T21368	det	The,results
R6435	T21368	T21369	nsubj	results,confirm
R6436	T21370	T21371	det	the,presence
R6437	T21371	T21369	dobj	presence,confirm
R6438	T21372	T21371	prep	of,presence
R6439	T21373	T21374	det	the,allele
R6440	T21374	T21372	pobj	allele,of
R6441	T21375	T21376	amod	wild,type
R6442	T21376	T21374	compound	type,allele
R6443	T21377	T21376	punct	-,type
R6444	T21378	T21371	prep	in,presence
R6445	T21379	T21380	det	all,cells
R6446	T21380	T21378	pobj	cells,in
R6447	T21381	T21369	punct	", ",confirm
R6448	T21382	T21383	mark	whereas,observed
R6449	T21383	T21369	advcl	observed,confirm
R6450	T21384	T21385	det	the,signal
R6451	T21385	T21383	nsubjpass	signal,observed
R6452	T21386	T21385	compound	mutant,signal
R6453	T21387	T21385	compound	allele,signal
R6454	T21388	T21383	auxpass	was,observed
R6455	T21389	T21390	advmod	only,in
R6456	T21390	T21383	prep	in,observed
R6457	T21391	T21392	det	the,clones
R6458	T21392	T21390	pobj	clones,in
R6459	T21393	T21392	nummod	two,clones
R6460	T21394	T21392	amod	targeted,clones
R6461	T21395	T21369	punct	.,confirm
R6462	T21397	T21398	punct	(,denotes
R6463	T21399	T21398	nsubj	M,denotes
R6464	T21400	T21398	punct	),denotes
R6465	T21401	T21402	det	the,marker
R6466	T21402	T21398	dobj	marker,denotes
R6467	T21403	T21404	amod	molecular,weight
R6468	T21404	T21402	compound	weight,marker
R6469	T21405	T21398	punct	.,denotes
R6470	T21857	T21858	amod	Nested,strategy
R6471	T21859	T21858	compound	PCR,strategy
R6472	T21860	T21858	prep	for,strategy
R6473	T21861	T21860	pobj	genotyping,for
R6474	T21862	T21861	prep	of,genotyping
R6475	T21863	T21864	amod	pre-implantation,embryos
R6476	T21864	T21862	pobj	embryos,of
R6477	T21865	T21858	punct	.,strategy
R6478	T21867	T21868	prep	Due,developed
R6479	T21869	T21867	pcomp	to,Due
R6480	T21870	T21871	det	the,scarcity
R6481	T21871	T21867	pobj	scarcity,Due
R6482	T21872	T21871	prep	of,scarcity
R6483	T21873	T21874	amod	extractable,material
R6484	T21874	T21872	pobj	material,of
R6485	T21875	T21874	amod	genetic,material
R6486	T21876	T21868	punct	", ",developed
R6487	T21877	T21878	det	a,strategy
R6488	T21878	T21868	nsubjpass	strategy,developed
R6489	T21879	T21878	amod	nested,strategy
R6490	T21880	T21878	compound	PCR,strategy
R6491	T21881	T21868	auxpass	was,developed
R6492	T21882	T21868	prep	in,developed
R6493	T21883	T21882	pobj	order,in
R6494	T21884	T21885	aux	to,genotype
R6495	T21885	T21883	acl	genotype,order
R6496	T21886	T21887	amod	pre-implantation,embryos
R6497	T21887	T21885	dobj	embryos,genotype
R6498	T21888	T21868	punct	.,developed
R6499	T21890	T21891	amod	Separate,reactions
R6500	T21891	T21892	nsubjpass	reactions,used
R6501	T21893	T21892	auxpass	were,used
R6502	T21894	T21895	aux	to,amplify
R6503	T21895	T21892	advcl	amplify,used
R6504	T21896	T21897	det	a,fragment
R6505	T21897	T21895	dobj	fragment,amplify
R6506	T21898	T21899	nummod	429,bp
R6507	T21899	T21897	compound	bp,fragment
R6508	T21900	T21897	prep	from,fragment
R6509	T21901	T21902	det	the,allele
R6510	T21902	T21900	pobj	allele,from
R6511	T21903	T21904	amod	wild,type
R6512	T21904	T21902	compound	type,allele
R6513	T21905	T21904	punct	-,type
R6514	T21906	T21897	cc	and,fragment
R6515	T21907	T21908	det	a,segment
R6516	T21908	T21897	conj	segment,fragment
R6517	T21909	T21910	nummod	389,bp
R6518	T21910	T21908	compound	bp,segment
R6519	T21911	T21908	prep	from,segment
R6520	T21912	T21913	det	the,allele
R6521	T21913	T21911	pobj	allele,from
R6522	T21914	T21913	compound	mutant,allele
R6523	T21915	T21897	punct	", ",fragment
R6524	T21916	T21897	appos	both,fragment
R6525	T21917	T21916	acl	spanning,both
R6526	T21918	T21919	det	the,end
R6527	T21919	T21917	dobj	end,spanning
R6528	T21920	T21919	nummod	3,end
R6529	T21921	T21920	punct	',3
R6530	T21922	T21919	prep	of,end
R6531	T21923	T21924	det	the,arm
R6532	T21924	T21922	pobj	arm,of
R6533	T21925	T21924	amod	short,arm
R6534	T21926	T21927	punct	(,upstream
R6535	T21927	T21924	parataxis	upstream,arm
R6536	T21928	T21927	punct	),upstream
R6537	T21929	T21924	prep	of,arm
R6538	T21930	T21931	det	the,vector
R6539	T21931	T21929	pobj	vector,of
R6540	T21932	T21931	compound	targeting,vector
R6541	T21933	T21892	punct	.,used
R6542	T21935	T21936	prep	In,co-amplified
R6543	T21937	T21938	det	both,reactions
R6544	T21938	T21935	pobj	reactions,In
R6545	T21939	T21936	punct	", ",co-amplified
R6546	T21940	T21941	det	a,sequence
R6547	T21941	T21936	nsubjpass	sequence,co-amplified
R6548	T21942	T21943	nummod	213,bp
R6549	T21943	T21941	compound	bp,sequence
R6550	T21944	T21941	acl	located,sequence
R6551	T21945	T21944	prep	within,located
R6552	T21946	T21947	det	the,arm
R6553	T21947	T21945	pobj	arm,within
R6554	T21948	T21947	amod	short,arm
R6555	T21949	T21936	auxpass	was,co-amplified
R6556	T21950	T21936	prep	with,co-amplified
R6557	T21951	T21952	det	the,products
R6558	T21952	T21950	pobj	products,with
R6559	T21953	T21952	punct	',products
R6560	T21954	T21952	amod	diagnostic,products
R6561	T21955	T21952	punct	',products
R6562	T21956	T21936	prep	as,co-amplified
R6563	T21957	T21958	det	an,control
R6564	T21958	T21956	pobj	control,as
R6565	T21959	T21958	amod	internal,control
R6566	T21960	T21936	punct	.,co-amplified
R6567	T21962	T21963	det	The,primers
R6568	T21963	T21966	nsubjpass	primers,used
R6569	T21964	T21963	amod	same,primers
R6570	T21965	T21963	compound	sense,primers
R6571	T21967	T21966	auxpass	were,used
R6572	T21968	T21969	aux	to,amplify
R6573	T21969	T21966	advcl	amplify,used
R6574	T21970	T21969	punct	',amplify
R6575	T21971	T21972	amod	diagnostic,sequences
R6576	T21972	T21969	dobj	sequences,amplify
R6577	T21973	T21972	punct	',sequences
R6578	T21974	T21972	prep	in,sequences
R6579	T21975	T21976	det	both,reactions
R6580	T21976	T21974	pobj	reactions,in
R6581	T21977	T21969	punct	", ",amplify
R6582	T21978	T21979	mark	whereas,were
R6583	T21979	T21969	advcl	were,amplify
R6584	T21980	T21981	det	the,primers
R6585	T21981	T21979	nsubj	primers,were
R6586	T21982	T21981	compound	antisense,primers
R6587	T21983	T21984	npadvmod	allele,specific
R6588	T21984	T21979	acomp	specific,were
R6589	T21985	T21984	punct	-,specific
R6590	T21986	T21966	punct	.,used
R6591	T21988	T21989	det	The,primers
R6592	T21989	T21990	nsubjpass	primers,depicted
R6593	T21991	T21989	punct	", ",primers
R6594	T21992	T21989	acl	represented,primers
R6595	T21993	T21992	agent	by,represented
R6596	T21994	T21993	pobj	triangles,by
R6597	T21995	T21990	punct	", ",depicted
R6598	T21996	T21990	auxpass	are,depicted
R6599	T21997	T21990	prep	in,depicted
R6600	T21998	T21999	nummod	two,sets
R6601	T21999	T21997	pobj	sets,in
R6602	T22000	T21999	punct	(,sets
R6603	T22001	T21999	amod	nested,sets
R6604	T22002	T21999	punct	),sets
R6605	T22003	T21999	prep	for,sets
R6606	T22004	T22003	pobj	each,for
R6607	T22005	T22004	prep	of,each
R6608	T22006	T22007	det	the,reactions
R6609	T22007	T22005	pobj	reactions,of
R6610	T22008	T22007	nummod	three,reactions
R6611	T22009	T21990	punct	.,depicted
R6612	T22011	T22012	nsubjpass	Exons,represented
R6613	T22012	T22014	ccomp	represented,cassette
R6614	T22013	T22012	auxpass	are,represented
R6615	T22015	T22012	agent	by,represented
R6616	T22016	T22017	amod	open,rectangles
R6617	T22017	T22015	pobj	rectangles,by
R6618	T22018	T22017	punct	", ",rectangles
R6619	T22019	T22017	amod	vertical,rectangles
R6620	T22020	T22014	punct	", ",cassette
R6621	T22021	T22014	det	the,cassette
R6622	T22022	T22023	compound	PGK,Neo
R6623	T22023	T22014	compound	Neo,cassette
R6624	T22024	T22023	punct	-,Neo
R6625	T22025	T22014	agent	by,cassette
R6626	T22026	T22027	det	an,rectangle
R6627	T22027	T22025	pobj	rectangle,by
R6628	T22028	T22027	amod	open,rectangle
R6629	T22029	T22027	punct	", ",rectangle
R6630	T22030	T22027	amod	horizontal,rectangle
R6631	T22031	T22014	punct	", ",cassette
R6632	T22032	T22033	mark	while,denote
R6633	T22033	T22014	advcl	denote,cassette
R6634	T22034	T22035	amod	thin,lines
R6635	T22035	T22033	nsubj	lines,denote
R6636	T22036	T22035	amod	vertical,lines
R6637	T22037	T22038	det	the,boundaries
R6638	T22038	T22033	dobj	boundaries,denote
R6639	T22039	T22038	prep	of,boundaries
R6640	T22040	T22041	det	the,arm
R6641	T22041	T22039	pobj	arm,of
R6642	T22042	T22041	amod	short,arm
R6643	T22043	T22038	cc	and,boundaries
R6644	T22044	T22045	det	the,end
R6645	T22045	T22038	conj	end,boundaries
R6646	T22046	T22045	nummod	5,end
R6647	T22047	T22046	punct	',5
R6648	T22048	T22045	prep	of,end
R6649	T22049	T22050	det	the,arm
R6650	T22050	T22048	pobj	arm,of
R6651	T22051	T22050	amod	long,arm
R6652	T22052	T22050	punct	(,arm
R6653	T22053	T22050	amod	downstream,arm
R6654	T22054	T22050	punct	),arm
R6655	T22055	T22014	punct	.,cassette
R6656	T22057	T22058	det	A,rectangle
R6657	T22058	T22062	nsubj	rectangle,delineates
R6658	T22059	T22058	amod	grey,rectangle
R6659	T22060	T22058	punct	", ",rectangle
R6660	T22061	T22058	amod	horizontal,rectangle
R6661	T22063	T22064	det	the,segment
R6662	T22064	T22062	dobj	segment,delineates
R6663	T22065	T22064	prep	of,segment
R6664	T22066	T22067	det	the,allele
R6665	T22067	T22065	pobj	allele,of
R6666	T22068	T22069	amod	wild,type
R6667	T22069	T22067	compound	type,allele
R6668	T22070	T22069	punct	-,type
R6669	T22071	T22072	dep	that,replaced
R6670	T22072	T22064	relcl	replaced,segment
R6671	T22073	T22072	auxpass	is,replaced
R6672	T22074	T22072	agent	by,replaced
R6673	T22075	T22076	det	the,cassette
R6674	T22076	T22074	pobj	cassette,by
R6675	T22077	T22078	compound	PGK,Neo
R6676	T22078	T22076	compound	Neo,cassette
R6677	T22079	T22078	punct	-,Neo
R6678	T22080	T22072	prep	in,replaced
R6679	T22081	T22082	det	the,allele
R6680	T22082	T22080	pobj	allele,in
R6681	T22083	T22082	compound	mutant,allele
R6682	T22084	T22062	punct	.,delineates
R6683	T22641	T22640	prep	of,Genotyping
R6684	T22642	T22643	amod	pre-implantation,embryos
R6685	T22643	T22641	pobj	embryos,of
R6686	T22644	T22640	punct	.,Genotyping
R6687	T22646	T22647	det	A,strategy
R6688	T22647	T22650	nsubjpass	strategy,used
R6689	T22648	T22647	amod	nested,strategy
R6690	T22649	T22647	compound	PCR,strategy
R6691	T22651	T22650	auxpass	was,used
R6692	T22652	T22653	aux	to,genotype
R6693	T22653	T22650	advcl	genotype,used
R6694	T22654	T22653	dobj	embryos,genotype
R6695	T22655	T22656	amod	prior,to
R6696	T22656	T22653	prep	to,genotype
R6697	T22657	T22656	pobj	implantation,to
R6698	T22658	T22659	mark	as,described
R6699	T22659	T22653	advcl	described,genotype
R6700	T22660	T22659	prep	in,described
R6701	T22661	T22660	pobj	Figure,in
R6702	T22662	T22661	nummod	4,Figure
R6703	T22663	T22650	punct	.,used
R6704	T22665	T22666	nmod	Capn2,mice
R6705	T22666	T22670	nsubjpass	mice,mated
R6706	T22667	T22665	punct	+,Capn2
R6707	T22668	T22665	punct	/,Capn2
R6708	T22669	T22665	punct	-,Capn2
R6709	T22671	T22670	auxpass	were,mated
R6710	T22672	T22670	cc	and,mated
R6711	T22673	T22674	det	the,date
R6712	T22674	T22675	nsubj	date,established
R6713	T22675	T22670	conj	established,mated
R6714	T22676	T22674	prep	of,date
R6715	T22677	T22676	pobj	fertilization,of
R6716	T22678	T22675	agent	by,established
R6717	T22679	T22680	det	the,appearance
R6718	T22680	T22678	pobj	appearance,by
R6719	T22681	T22680	prep	of,appearance
R6720	T22682	T22683	amod	vaginal,plugs
R6721	T22683	T22681	pobj	plugs,of
R6722	T22684	T22675	punct	.,established
R6723	T22686	T22687	nmod	Blastocyst,embryos
R6724	T22687	T22695	nsubjpass	embryos,flushed
R6725	T22688	T22689	punct	(,E3.5
R6726	T22689	T22686	parataxis	E3.5,Blastocyst
R6727	T22690	T22689	punct	),E3.5
R6728	T22691	T22686	cc	or,Blastocyst
R6729	T22692	T22693	nummod	8,cell
R6730	T22693	T22686	conj	cell,Blastocyst
R6731	T22694	T22693	punct	-,cell
R6732	T22696	T22697	punct	(,E2.5
R6733	T22697	T22687	parataxis	E2.5,embryos
R6734	T22698	T22697	punct	),E2.5
R6735	T22699	T22695	auxpass	were,flushed
R6736	T22700	T22695	prep	from,flushed
R6737	T22701	T22702	det	the,oviducts
R6738	T22702	T22700	pobj	oviducts,from
R6739	T22703	T22695	cc	and,flushed
R6740	T22704	T22705	advmod	then,digested
R6741	T22705	T22695	conj	digested,flushed
R6742	T22706	T22705	prep	with,digested
R6743	T22707	T22708	compound	proteinase,K
R6744	T22708	T22706	pobj	K,with
R6745	T22709	T22695	punct	.,flushed
R6746	T22711	T22712	prep	In,co-amplified
R6747	T22713	T22714	amod	separate,segments
R6748	T22714	T22711	pobj	segments,In
R6749	T22715	T22714	compound	reactions,segments
R6750	T22716	T22714	acl	found,segments
R6751	T22717	T22716	advmod	exclusively,found
R6752	T22718	T22716	prep	in,found
R6753	T22719	T22720	preconj	either,type
R6754	T22720	T22718	pobj	type,in
R6755	T22721	T22720	det	the,type
R6756	T22722	T22720	amod	wild,type
R6757	T22723	T22720	punct	-,type
R6758	T22724	T22720	cc	or,type
R6759	T22725	T22720	conj	mutant,type
R6760	T22726	T22712	nsubjpass	alleles,co-amplified
R6761	T22727	T22712	auxpass	were,co-amplified
R6762	T22728	T22712	prep	with,co-amplified
R6763	T22729	T22730	det	an,sequence
R6764	T22730	T22728	pobj	sequence,with
R6765	T22731	T22730	amod	internal,sequence
R6766	T22732	T22730	compound	control,sequence
R6767	T22733	T22730	punct	", ",sequence
R6768	T22734	T22730	acl	located,sequence
R6769	T22735	T22734	prep	in,located
R6770	T22736	T22737	det	the,arm
R6771	T22737	T22735	pobj	arm,in
R6772	T22738	T22737	amod	short,arm
R6773	T22739	T22737	punct	(,arm
R6774	T22740	T22737	amod	upstream,arm
R6775	T22741	T22737	punct	),arm
R6776	T22742	T22737	prep	of,arm
R6777	T22743	T22744	det	the,vector
R6778	T22744	T22742	pobj	vector,of
R6779	T22745	T22744	compound	targeting,vector
R6780	T22746	T22744	punct	", ",vector
R6781	T22747	T22748	dep	which,found
R6782	T22748	T22744	relcl	found,vector
R6783	T22749	T22748	auxpass	is,found
R6784	T22750	T22748	prep	in,found
R6785	T22751	T22752	det	both,alleles
R6786	T22752	T22750	pobj	alleles,in
R6787	T22753	T22712	punct	.,co-amplified
R6788	T22755	T22756	det	The,products
R6789	T22756	T22758	nsubj	products,were
R6790	T22757	T22756	amod	final,products
R6791	T22759	T22760	nummod	429,bp
R6792	T22760	T22758	attr	bp,were
R6793	T22761	T22758	prep	for,were
R6794	T22762	T22763	det	the,allele
R6795	T22763	T22761	pobj	allele,for
R6796	T22764	T22765	amod	wild,type
R6797	T22765	T22763	compound	type,allele
R6798	T22766	T22765	punct	-,type
R6799	T22767	T22758	punct	", ",were
R6800	T22768	T22769	nummod	389,bp
R6801	T22769	T22758	conj	bp,were
R6802	T22770	T22769	prep	for,bp
R6803	T22771	T22772	det	the,allele
R6804	T22772	T22770	pobj	allele,for
R6805	T22773	T22772	compound	mutant,allele
R6806	T22774	T22769	punct	", ",bp
R6807	T22775	T22769	cc	and,bp
R6808	T22776	T22777	nummod	213,bp
R6809	T22777	T22769	conj	bp,bp
R6810	T22778	T22777	prep	for,bp
R6811	T22779	T22780	det	the,control
R6812	T22780	T22778	pobj	control,for
R6813	T22781	T22780	amod	internal,control
R6814	T22782	T22758	punct	.,were
R6815	T22784	T22785	punct	(,A
R6816	T22785	T22786	meta	A,example
R6817	T22787	T22785	punct	),A
R6818	T22788	T22786	det	A,example
R6819	T22789	T22786	amod	representative,example
R6820	T22790	T22786	prep	of,example
R6821	T22791	T22792	det	the,genotyping
R6822	T22792	T22790	pobj	genotyping,of
R6823	T22793	T22792	prep	of,genotyping
R6824	T22794	T22795	compound	blastocyst,embryos
R6825	T22795	T22793	pobj	embryos,of
R6826	T22796	T22795	compound	stage,embryos
R6827	T22797	T22786	punct	.,example
R6828	T22799	T22800	nmod	Embryos,1
R6829	T22800	T22802	nsubj	1,were
R6830	T22801	T22800	punct	#,1
R6831	T22803	T22800	punct	", ",1
R6832	T22804	T22800	conj	2,1
R6833	T22805	T22804	punct	", ",2
R6834	T22806	T22804	conj	4,2
R6835	T22807	T22806	punct	", ",4
R6836	T22808	T22806	conj	5,4
R6837	T22809	T22808	punct	", ",5
R6838	T22810	T22808	cc	and,5
R6839	T22811	T22808	conj	6,5
R6840	T22812	T22802	attr	Capn2,were
R6841	T22813	T22812	punct	+,Capn2
R6842	T22814	T22812	punct	/,Capn2
R6843	T22815	T22812	punct	-,Capn2
R6844	T22816	T22817	mark	whereas,were
R6845	T22817	T22802	advcl	were,were
R6846	T22818	T22819	nmod	embryos,3
R6847	T22819	T22817	nsubj	3,were
R6848	T22820	T22819	punct	#,3
R6849	T22821	T22819	cc	and,3
R6850	T22822	T22823	punct	#,7
R6851	T22823	T22819	conj	7,3
R6852	T22824	T22817	attr	Capn2,were
R6853	T22825	T22824	punct	+,Capn2
R6854	T22826	T22824	punct	/,Capn2
R6855	T22827	T22824	punct	+,Capn2
R6856	T22828	T22817	punct	", ",were
R6857	T22829	T22817	advcl	denoted,were
R6858	T22830	T22829	agent	by,denoted
R6859	T22831	T22832	det	the,absence
R6860	T22832	T22830	pobj	absence,by
R6861	T22833	T22832	prep	of,absence
R6862	T22834	T22835	det	the,signal
R6863	T22835	T22833	pobj	signal,of
R6864	T22836	T22837	nummod	389,bp
R6865	T22837	T22835	compound	bp,signal
R6866	T22838	T22835	compound	mutant,signal
R6867	T22839	T22802	punct	.,were
R6868	T22841	T22842	punct	(,B
R6869	T22842	T22843	meta	B,shown
R6870	T22844	T22842	punct	),B
R6871	T22845	T22846	det	An,example
R6872	T22846	T22843	nsubjpass	example,shown
R6873	T22847	T22846	prep	of,example
R6874	T22848	T22849	det	the,genotyping
R6875	T22849	T22847	pobj	genotyping,of
R6876	T22850	T22849	prep	of,genotyping
R6877	T22851	T22852	nummod	8,cell
R6878	T22852	T22854	compound	cell,embryos
R6879	T22853	T22852	punct	-,cell
R6880	T22854	T22850	pobj	embryos,of
R6881	T22855	T22843	auxpass	is,shown
R6882	T22856	T22843	punct	.,shown
R6883	T22858	T22859	nmod	Embryos,1
R6884	T22859	T22861	nsubj	1,were
R6885	T22860	T22859	punct	#,1
R6886	T22862	T22859	punct	", ",1
R6887	T22863	T22859	conj	2,1
R6888	T22864	T22863	punct	", ",2
R6889	T22865	T22863	conj	3,2
R6890	T22866	T22865	punct	", ",3
R6891	T22867	T22865	conj	5,3
R6892	T22868	T22867	punct	", ",5
R6893	T22869	T22867	cc	and,5
R6894	T22870	T22867	conj	6,5
R6895	T22871	T22861	attr	Capn2,were
R6896	T22872	T22871	punct	+,Capn2
R6897	T22873	T22871	punct	/,Capn2
R6898	T22874	T22871	punct	-,Capn2
R6899	T22875	T22876	mark	while,was
R6900	T22876	T22861	advcl	was,were
R6901	T22877	T22876	nsubj	embryo,was
R6902	T22878	T22877	punct	#,embryo
R6903	T22879	T22877	nummod	4,embryo
R6904	T22880	T22876	attr	Capn2,was
R6905	T22881	T22880	punct	-,Capn2
R6906	T22882	T22880	punct	/,Capn2
R6907	T22883	T22880	punct	-,Capn2
R6908	T22884	T22880	punct	", ",Capn2
R6909	T22885	T22880	acl	marked,Capn2
R6910	T22886	T22885	agent	by,marked
R6911	T22887	T22888	det	the,absence
R6912	T22888	T22886	pobj	absence,by
R6913	T22889	T22888	prep	of,absence
R6914	T22890	T22891	det	the,signal
R6915	T22891	T22889	pobj	signal,of
R6916	T22892	T22893	nummod	429,bp
R6917	T22893	T22891	nmod	bp,signal
R6918	T22894	T22895	amod	wild,type
R6919	T22895	T22891	compound	type,signal
R6920	T22896	T22895	punct	-,type
R6921	T22897	T22861	punct	.,were
R6922	T22899	T22900	punct	(,M
R6923	T22900	T22901	nsubj	M,denotes
R6924	T22902	T22900	punct	),M
R6925	T22903	T22904	det	the,marker
R6926	T22904	T22901	dobj	marker,denotes
R6927	T22905	T22906	amod	molecular,weight
R6928	T22906	T22904	compound	weight,marker
R6929	T22907	T22901	punct	.,denotes
R6930	T22936	T22937	compound	Genotype,distribution
R6931	T22938	T22937	prep	of,distribution
R6932	T22939	T22938	pobj	offspring,of
R6933	T22940	T22939	acl	derived,offspring
R6934	T22941	T22940	prep	from,derived
R6935	T22942	T22943	nmod	Capn2,mice
R6936	T22943	T22941	pobj	mice,from
R6937	T22944	T22943	amod	transgenic,mice
R6938	T22945	T22937	punct	.,distribution
R1801	T6919	T6920	nsubj	None,were
R1881	T7005	T7006	det	"Table 2 Genotype distribution of offspring derived from Capn2 transgenic mice. Cross (N) Age Genotype +/+ +/- -/- ND* (+/-)♀ × (+/-)♂ 199 3 weeks 23 176 0 0 (+/+)♀ × (+/-)♂ 122 3 weeks 50 72 0 0 (+/-)♀ × (+/+)♂ 103 3 weeks 28 75 0 0 (+/-)♀ × (+/-)♂ 9 E18.5 0 7 0 2 (+/-)♀ × (+/-)♂ 5 E17.5 0 5 0 0 (+/-)♀ × (+/-)♂ 9 E14.5 2 7 0 0 (+/-)♀ × (+/-)♂ 10 E11.5 3 7 0 0 (+/-)♀ × (+/-)♂ 7 E10.5 2 5 0 0 (+/-)♀ × (+/-)♂ 46 E3.5 7 39 0 0 (+/-)♀ × (+/-)♂ 48 E2.5 8 34 2 4 * ND, not determined Figure 4 Nested PCR strategy for genotyping of pre-implantation embryos. Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos. Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector. In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control. The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific. The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions. Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm. A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele. Figure 5 Genotyping of pre-implantation embryos. A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4. Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs. Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K. In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles. The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control. (A) A representative example of the genotyping of blastocyst stage embryos. Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal. (B) An example of the genotyping of 8-cell embryos is shown. Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal. (M) denotes the molecular weight marker. The",genotyping
R1882	T7006	T7007	nsubjpass	genotyping,shown
R1883	T7008	T7006	nmod	results,genotyping
R1884	T7009	T7006	prep	for,genotyping
R1885	T7010	T7009	pobj	weanlings,for
R1886	T7011	T7010	punct	", ",weanlings
R1887	T7012	T7013	nmod	post-implantation,embryos
R1888	T7013	T7010	appos	embryos,weanlings
R1889	T7014	T7012	punct	", ",post-implantation
R1890	T7015	T7012	cc	and,post-implantation
R1891	T7016	T7012	conj	pre-implantation,post-implantation

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T22582	7076-7078	SO:0000028	denotes	bp
T22581	7033-7038	PR:000005015	denotes	Capn2
T22580	7019-7025	UBERON:0000922	denotes	embryo
T22579	7004-7009	PR:000005015	denotes	Capn2
T22578	6972-6979	UBERON:0000922	denotes	Embryos
T22577	6947-6961	UBERON:0019252	denotes	8-cell embryos
T22576	6893-6895	SO:0000028	denotes	bp
T22575	6849-6854	PR:000005015	denotes	Capn2
T22574	6826-6833	UBERON:0000922	denotes	embryos
T22573	6809-6814	PR:000005015	denotes	Capn2
T22572	6777-6784	UBERON:0000922	denotes	Embryos
T22571	6768-6775	UBERON:0000922	denotes	embryos
T22570	6751-6761	UBERON:0000358	denotes	blastocyst
T22569	6672-6674	SO:0000028	denotes	bp
T22568	6656-6662	SO:0001023	denotes	allele
T22567	6638-6640	SO:0000028	denotes	bp
T22566	6626-6632	SO:0001023	denotes	allele
T22565	6605-6607	SO:0000028	denotes	bp
T22564	6568-6575	SO:0001023	denotes	alleles
T22563	6527-6543	SO:0001644	denotes	targeting vector
T22562	6423-6430	SO:0001023	denotes	alleles
T22561	6294-6302	UBERON:0000993	denotes	oviducts
T22560	6250-6264	UBERON:0019252	denotes	8-cell embryos
T22559	6229-6239	UBERON:0000358	denotes	Blastocyst
T22558	6214-6227	UBERON:0010148	denotes	vaginal plugs
T22557	6167-6180	GO:0009566	denotes	fertilization
T22556	6145-6150	GO:0007618	denotes	mated
T22555	6135-6139	NCBITaxon:10088	denotes	mice
T22554	6126-6131	PR:000005015	denotes	Capn2
T22553	6087-6099	GO:0007566	denotes	implantation
T22552	6070-6077	UBERON:0000922	denotes	embryos
T22551	6018-6025	UBERON:0000922	denotes	embryos
T22550	6005-6017	GO:0007566	denotes	implantation
T21822	5969-5975	SO:0001023	denotes	allele
T21821	5946-5954	SO:0005853	denotes	cassette
T21820	5907-5913	SO:0001023	denotes	allele
T21819	5685-5693	SO:0005853	denotes	cassette
T21818	5621-5626	SO:0000147	denotes	Exons
T21817	5519-5526	SO:0000112	denotes	primers
T21816	5498-5504	SO:0001023	denotes	allele
T21815	5485-5492	SO:0000112	denotes	primers
T21814	5475-5484	SO:0000077	denotes	antisense
T21813	5392-5399	SO:0000112	denotes	primers
T21812	5264-5266	SO:0000028	denotes	bp
T21811	5221-5237	SO:0001644	denotes	targeting vector
T21810	5153-5159	SO:0001023	denotes	allele
T21809	5126-5128	SO:0000028	denotes	bp
T21808	5109-5115	SO:0001023	denotes	allele
T21807	5078-5080	SO:0000028	denotes	bp
T21806	5023-5030	UBERON:0000922	denotes	embryos
T21805	5010-5022	GO:0007566	denotes	implantation
T4751	43-48	PR:000005015	denotes	Capn2
T4752	58-65	CL:0002322	denotes	ES cell
T4753	89-94	PR:000005015	denotes	Capn2
T4754	98-105	CL:0002322	denotes	ES cell
T4755	174-178	CHEBI:23888	denotes	drug
T4756	222-227	PR:000005015	denotes	Capn2
T4757	272-285	GO:0097617	denotes	hybridization
T4758	365-375	GO:0097617	denotes	hybridized
T4759	388-393	PR:P23940	denotes	BamHI
T4760	420-426	SO:0001023	denotes	allele
T4761	438-443	PR:P23940	denotes	BamHI
T4762	467-473	SO:0001023	denotes	allele
T4763	492-499	SO:0001026	denotes	genomic
T4764	776-784	SO:0005853	denotes	cassette
T4765	812-817	PR:P23940	denotes	BamHI
T4766	830-835	PR:000005015	denotes	Capn2
T4767	839-847	CL:0002322	denotes	ES cells
T4768	869-885	SO:0001644	denotes	targeting vector
T4769	915-920	PR:000005015	denotes	Capn2
T4770	1028-1030	SO:0000028	denotes	bp
T4771	1043-1045	SO:0000028	denotes	bp
T4772	1070-1076	SO:0001023	denotes	allele
T4773	1088-1090	SO:0000028	denotes	bp
T6380	2478-2482	NCBITaxon:10088	denotes	mice
T6381	2523-2528	PR:000005015	denotes	Capn2
T6382	2529-2535	SO:0001023	denotes	allele
T6383	2556-2560	NCBITaxon:10088	denotes	mice
T6384	2578-2584	UBERON:0000085	denotes	morula
T6385	2619-2624	PR:000005015	denotes	Capn2
T6386	2679-2684	PR:000005015	denotes	Capn2
T6387	2760-2765	PR:000005015	denotes	Capn2
T6388	2769-2776	NCBITaxon:33208	denotes	animals
T6389	2827-2834	UBERON:0001062	denotes	anatomy
T6390	2836-2848	GO:0000003	denotes	reproduction
T6391	2853-2862	UBERON:0000104	denotes	life span
T6392	2920-2925	PR:000005015	denotes	Capn2
T6393	2995-3004	UBERON:0012101	denotes	perinatal
T6394	2999-3004	GO:0007567	denotes	natal
T6395	3005-3010	GO:0016265	denotes	death
T6396	3019-3024	PR:000005015	denotes	Capn2
T6397	3074-3079	PR:000005015	denotes	Capn2
T6398	3083-3090	NCBITaxon:33208	denotes	animals
T6399	3091-3099	GO:0016265	denotes	perished
T6400	3121-3130	UBERON:0000922	denotes	embryonic
T6401	3121-3142	GO:0009790	denotes	embryonic development
T6402	3174-3183	UBERON:0000922	denotes	embryonic
T6403	3184-3189	GO:0016265	denotes	death
T6404	3209-3221	GO:0007566	denotes	implantation
T6405	3229-3236	UBERON:0000922	denotes	embryos
T6406	3314-3319	PR:000005015	denotes	Capn2
T6407	3323-3330	UBERON:0000922	denotes	embryos
T6408	3361-3367	UBERON:0000922	denotes	embryo
T6409	3428-3433	PR:000005015	denotes	Capn2
T6410	3437-3444	UBERON:0000922	denotes	embryos
T6411	3454-3459	GO:0016265	denotes	dying
T6412	3469-3481	GO:0007566	denotes	implantation
T6413	3483-3490	UBERON:0000922	denotes	Embryos
T6414	3518-3526	UBERON:0000993	denotes	oviducts
T6415	3530-3538	GO:0007565	denotes	pregnant
T6416	3661-3673	GO:0007566	denotes	implantation
T6417	3674-3681	UBERON:0000922	denotes	embryos
T6418	3687-3692	PR:000005015	denotes	Capn2
T6419	3732-3737	PR:000005015	denotes	Capn2
T6420	3741-3748	UBERON:0000922	denotes	embryos
T6421	3770-3782	UBERON:0019252	denotes	8-cell stage
T6422	3876-3886	UBERON:0000358	denotes	blastocyst
T6423	3894-3901	UBERON:0000922	denotes	embryos
T6424	3907-3912	PR:000005015	denotes	Capn2
T6425	3933-3938	PR:000005015	denotes	Capn2
T6426	3949-3956	UBERON:0000922	denotes	embryos
T6427	3974-3986	UBERON:0019252	denotes	8-cell stage
T6428	4074-4087	UBERON:0019248	denotes	early embryos
T6429	4166-4176	SO:0000673	denotes	transcript
T6430	4225-4230	PR:000005015	denotes	Capn2
T6431	4234-4241	UBERON:0000922	denotes	embryos
T7655	9359-9366	CL:0002322	denotes	ES cell
T7654	9242-9254	UBERON:0019252	denotes	8-cell stage
T7653	9223-9230	UBERON:0000922	denotes	embryos
T7652	9214-9219	PR:000005015	denotes	Capn2
T7651	9173-9181	CL:0002322	denotes	ES cells
T7650	9164-9169	PR:000005015	denotes	Capn2
T7649	9116-9120	CHEBI:23888	denotes	drug
T7648	9075-9083	CL:0002322	denotes	ES cells
T7647	9066-9071	PR:000005015	denotes	Capn2
T7646	9025-9033	CL:0002322	denotes	ES cells
T7645	9016-9021	PR:000005022	denotes	Capn4
T7644	8967-8975	CL:0002322	denotes	ES cells
T7643	8894-8909	GO:0035822	denotes	gene conversion
T7642	8894-8898	SO:0000704	denotes	gene
T7641	8838-8842	CHEBI:42768	denotes	G418
T7640	8767-8775	CL:0002322	denotes	ES cells
T7639	8758-8763	PR:000005015	denotes	Capn2
T7638	8749-8757	CL:0002322	denotes	ES cells
T7637	8740-8745	PR:000005015	denotes	Capn2
T7636	8752-8757	GO:0048468	denotes	cells
T21060	1199-1206	CL:0002322	denotes	ES cell
T21061	1245-1250	PR:000005015	denotes	Capn2
T21062	1330-1335	PR:P23940	denotes	BamHI
T21063	1345-1352	SO:0001026	denotes	genomic
T21064	1372-1380	CL:0002322	denotes	ES cells
T21065	1385-1395	GO:0097617	denotes	hybridized
T21066	1403-1406	CHEBI:42098	denotes	DIG
T21067	1419-1421	SO:0000028	denotes	bp
T21068	1422-1427	PR:P23940	denotes	BamHI
T21069	1428-1435	PR:P43870	denotes	HindIII
T21070	1498-1514	SO:0001644	denotes	targeting vector
T21071	1536-1541	PR:P23940	denotes	BamHI
T21072	1582-1588	SO:0001023	denotes	allele
T21073	1657-1663	SO:0001023	denotes	allele
T21074	1829-1831	SO:0000028	denotes	bp
T21075	1859-1865	SO:0001023	denotes	allele
T21076	1877-1879	SO:0000028	denotes	bp
T21077	1904-1910	SO:0001023	denotes	allele
T21078	1947-1953	SO:0000112	denotes	primer
T21079	1965-1971	SO:0000188	denotes	intron
T21080	2004-2020	SO:0001644	denotes	targeting vector
T21081	2035-2041	SO:0001023	denotes	allele
T21082	2051-2060	SO:0000077	denotes	antisense
T21083	2061-2068	SO:0000112	denotes	primers
T21084	2107-2113	SO:0001023	denotes	allele
T21085	2122-2131	SO:0000077	denotes	antisense
T21086	2132-2138	SO:0000112	denotes	primer
T21087	2150-2154	SO:0000147	denotes	exon
T21088	2221-2230	SO:0000077	denotes	antisense
T21089	2231-2237	SO:0000112	denotes	primer
T21090	2253-2261	SO:0005853	denotes	cassette
T21091	2313-2319	SO:0001023	denotes	allele
T21092	2353-2359	SO:0001023	denotes	allele
T21804	4931-4938	SO:0000704	denotes	genetic
T21803	4887-4894	UBERON:0000922	denotes	embryos
T21802	4874-4886	GO:0007566	denotes	implantation
T22930	4331-4336	PR:000005015	denotes	Capn2
T22931	4348-4352	NCBITaxon:10088	denotes	mice
T6447	7834-7841	NCBITaxon:33208	denotes	animals
T6446	7825-7830	PR:000005015	denotes	Capn2
T6445	7576-7583	NCBITaxon:33208	denotes	animals
T6444	7567-7572	PR:000005015	denotes	Capn2
T6443	7555-7560	PR:000005015	denotes	Capn2
T6442	7519-7526	NCBITaxon:33208	denotes	animals
T6441	7510-7515	PR:000005015	denotes	Capn2
T6440	7477-7482	PR:000005015	denotes	Capn2
T6439	7417-7424	NCBITaxon:33208	denotes	animals
T6438	7290-7297	NCBITaxon:33208	denotes	animals
T6437	7282-7287	PR:000005015	denotes	Capn2
T6436	7270-7275	PR:000005015	denotes	Capn2
T6435	7216-7223	UBERON:0000922	denotes	embryos
T6434	7203-7215	GO:0007566	denotes	implantation
T6433	7181-7193	GO:0007566	denotes	implantation
T7635	8726-8739	_FRAGMENT	denotes	generation of
T6466	8686-8692	SO:0001023	denotes	allele
T6465	8661-8666	PR:000005015	denotes	Capn2
T6464	8591-8597	SO:0001023	denotes	allele
T6463	8566-8571	PR:000005015	denotes	Capn2
T6462	8440-8447	NCBITaxon:33208	denotes	animals
T6461	8431-8436	PR:000005015	denotes	Capn2
T6460	8419-8424	PR:000005015	denotes	Capn2
T6459	8311-8317	SO:0001023	denotes	allele
T6458	8257-8262	PR:000005015	denotes	Capn2
T6457	8213-8220	NCBITaxon:33208	denotes	animals
T6456	8204-8209	PR:000005015	denotes	Capn2
T6455	8141-8148	NCBITaxon:33208	denotes	animals
T6454	8132-8137	PR:000005015	denotes	Capn2
T6453	8119-8124	PR:000005015	denotes	Capn2
T6452	8026-8031	PR:000005022	denotes	Capn4
T6451	7986-7993	NCBITaxon:33208	denotes	animals
T6450	7906-7913	UBERON:0000922	denotes	embryos
T6449	7893-7905	GO:0007566	denotes	implantation
T6448	7872-7884	GO:0007566	denotes	implantation
T6432	4260-4266	UBERON:0000085	denotes	morula
R2219	T7636	T7635	_lexicallyChainedTo	cells,generation of

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T22639	7130-7136	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	marker
T22638	7113-7122	CHEBI_EXT:polyatomic_entity_or_group	denotes	molecular
T22637	7098-7099	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	M
T22636	7079-7088	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T22635	7076-7078	SO_EXT:0000028	denotes	bp
T22634	7040-7041	SO_EXT:sequence_nullness_or_absence	denotes	-
T22633	7038-7039	SO_EXT:sequence_nullness_or_absence	denotes	-
T22632	7033-7038	PR_EXT:000005015	denotes	Capn2
T22631	7019-7025	UBERON:0000922	denotes	embryo
T22630	7011-7012	SO_EXT:sequence_nullness_or_absence	denotes	-
T22629	7009-7010	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22628	7004-7009	PR_EXT:000005015	denotes	Capn2
T22627	6972-6979	UBERON:0000922	denotes	Embryos
T22626	6949-6953	CL_GO_EXT:cell	denotes	cell
T22625	6947-6961	UBERON:0019252	denotes	8-cell embryos
T22624	6896-6902	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T22623	6893-6895	SO_EXT:0000028	denotes	bp
T22622	6856-6857	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22621	6854-6855	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22620	6849-6854	PR_EXT:000005015	denotes	Capn2
T22603	6506-6514	SO_EXT:sequence_upstreamness	denotes	upstream
T22602	6474-6482	SO_EXT:biological_sequence	denotes	sequence
T22601	6423-6430	SO_EXT:0001023	denotes	alleles
T22600	6416-6422	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T22599	6403-6412	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T22598	6326-6336	GO_EXT:0008233	denotes	proteinase
T22597	6294-6302	UBERON:0000993	denotes	oviducts
T22596	6252-6256	CL_GO_EXT:cell	denotes	cell
T22595	6250-6264	UBERON:0019252	denotes	8-cell embryos
T22594	6229-6239	UBERON:0000358	denotes	Blastocyst
T22593	6214-6227	UBERON:0010148	denotes	vaginal plugs
T22592	6167-6180	GO:0009566	denotes	fertilization
T22591	6145-6150	GO:0007618	denotes	mated
T22590	6135-6139	NCBITaxon:10088	denotes	mice
T22589	6133-6134	SO_EXT:sequence_nullness_or_absence	denotes	-
T22588	6131-6132	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22587	6126-6131	PR_EXT:000005015	denotes	Capn2
T22586	6087-6099	GO:0007566	denotes	implantation
T22585	6070-6077	UBERON:0000922	denotes	embryos
T22584	6018-6025	UBERON:0000922	denotes	embryos
T22583	6005-6017	GO:0007566	denotes	implantation
T21834	5200-5208	SO_EXT:sequence_upstreamness	denotes	upstream
T21833	5153-5159	SO_EXT:0001023	denotes	allele
T21832	5146-5152	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T21831	5126-5128	SO_EXT:0000028	denotes	bp
T21830	5109-5115	SO_EXT:0001023	denotes	allele
T21829	5099-5108	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T21828	5078-5080	SO_EXT:0000028	denotes	bp
T21827	5023-5030	UBERON:0000922	denotes	embryos
T21826	5010-5022	GO:0007566	denotes	implantation
T21825	4931-4938	SO_EXT:0000704	denotes	genetic
T21824	4887-4894	UBERON:0000922	denotes	embryos
T21823	4874-4886	GO:0007566	denotes	implantation
T21856	5969-5975	SO_EXT:0001023	denotes	allele
T21855	5962-5968	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T21854	5946-5954	SO_EXT:0005853	denotes	cassette
T21853	5942-5945	CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase	denotes	Neo
T21852	5938-5941	GO_EXT:phosphoglycerate_kinase	denotes	PGK
T21851	5922-5930	SO_EXT:sequence_substitution_process	denotes	replaced
T21850	5907-5913	SO_EXT:0001023	denotes	allele
T21849	5897-5906	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T21848	5821-5831	SO_EXT:sequence_downstreamness	denotes	downstream
T21847	5685-5693	SO_EXT:0005853	denotes	cassette
T21846	5681-5684	CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase	denotes	Neo
T21845	5677-5680	GO_EXT:phosphoglycerate_kinase	denotes	PGK
T21844	5621-5626	SO_EXT:0000147	denotes	Exons
T21843	5519-5526	SO_EXT:0000112	denotes	primers
T21842	5498-5504	SO_EXT:0001023	denotes	allele
T21841	5485-5492	SO_EXT:0000112	denotes	primers
T21840	5475-5484	SO:0000077	denotes	antisense
T21839	5434-5443	SO_EXT:biological_sequence	denotes	sequences
T21838	5392-5399	SO_EXT:0000112	denotes	primers
T21837	5267-5275	SO_EXT:biological_sequence	denotes	sequence
T21836	5264-5266	SO_EXT:0000028	denotes	bp
T21835	5221-5237	SO_EXT:0001644	denotes	targeting vector
T7703	9367-9373	SO_EXT:sequence_cloned_entity	denotes	clones
T7702	9362-9366	CL_GO_EXT:cell	denotes	cell
T7701	9359-9366	CL:0002322	denotes	ES cell
T7700	9313-9317	CL_GO_EXT:cell	denotes	cell
T7699	9271-9280	GO_PR_EXT:calpain_2_complex	denotes	m-calpain
T7698	9244-9248	CL_GO_EXT:cell	denotes	cell
T7697	9242-9254	UBERON:0019252	denotes	8-cell stage
T7696	9223-9230	UBERON:0000922	denotes	embryos
T7695	9221-9222	SO_EXT:sequence_nullness_or_absence	denotes	-
T7694	9219-9220	SO_EXT:sequence_nullness_or_absence	denotes	-
T7693	9214-9219	PR_EXT:000005015	denotes	Capn2
T7692	9176-9181	CL_GO_EXT:cell	denotes	cells
T7691	9173-9181	CL:0002322	denotes	ES cells
T7690	9171-9172	SO_EXT:sequence_nullness_or_absence	denotes	-
T7689	9169-9170	SO_EXT:sequence_nullness_or_absence	denotes	-
T7688	9164-9169	PR_EXT:000005015	denotes	Capn2
T7687	9131-9137	SO_EXT:sequence_cloned_entity	denotes	clones
T7686	9116-9120	CHEBI_EXT:23888	denotes	drug
T7685	9078-9083	CL_GO_EXT:cell	denotes	cells
T7684	9075-9083	CL:0002322	denotes	ES cells
T7683	9073-9074	SO_EXT:sequence_nullness_or_absence	denotes	-
T7682	9071-9072	SO_EXT:sequence_nullness_or_absence	denotes	-
T7681	9066-9071	PR_EXT:000005015	denotes	Capn2
T6634	8686-8692	SO_EXT:0001023	denotes	allele
T6633	8679-8685	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6632	8668-8669	SO_EXT:sequence_nullness_or_absence	denotes	-
T6631	8666-8667	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6630	8661-8666	PR_EXT:000005015	denotes	Capn2
T6629	8648-8655	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	females
T6628	8631-8636	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	males
T6627	8591-8597	SO_EXT:0001023	denotes	allele
T6626	8584-8590	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6625	8573-8574	SO_EXT:sequence_nullness_or_absence	denotes	-
T6624	8571-8572	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6623	8566-8571	PR_EXT:000005015	denotes	Capn2
T6622	8553-8560	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	females
T6621	8536-8541	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	males
T6620	8463-8470	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	females
T6619	8454-8459	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	males
T6618	8440-8447	NCBITaxon:33208	denotes	animals
T6617	8438-8439	SO_EXT:sequence_nullness_or_absence	denotes	-
T6616	8436-8437	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6615	8431-8436	PR_EXT:000005015	denotes	Capn2
T6614	8426-8427	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6613	8424-8425	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6612	8419-8424	PR_EXT:000005015	denotes	Capn2
T6611	8311-8317	SO_EXT:0001023	denotes	allele
T6607	8257-8262	PR_EXT:000005015	denotes	Capn2
T6606	8213-8220	NCBITaxon:33208	denotes	animals
T6605	8211-8212	SO_EXT:sequence_nullness_or_absence	denotes	-
T6604	8209-8210	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6603	8204-8209	PR_EXT:000005015	denotes	Capn2
T6602	8141-8148	NCBITaxon:33208	denotes	animals
T6601	8139-8140	SO_EXT:sequence_nullness_or_absence	denotes	-
T6600	8137-8138	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6599	8132-8137	PR_EXT:000005015	denotes	Capn2
T6598	8126-8127	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6597	8124-8125	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6596	8119-8124	PR_EXT:000005015	denotes	Capn2
T6595	8061-8069	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T6594	8032-8042	SO_EXT:transgenic_entity	denotes	transgenic
T6593	8026-8031	PR_EXT:000005022	denotes	Capn4
T6592	7986-7993	NCBITaxon:33208	denotes	animals
T6591	7906-7913	UBERON:0000922	denotes	embryos
T6589	7872-7884	GO:0007566	denotes	implantation
T6588	7834-7841	NCBITaxon:33208	denotes	animals
T6587	7832-7833	SO_EXT:sequence_nullness_or_absence	denotes	-
T6586	7830-7831	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6585	7825-7830	PR_EXT:000005015	denotes	Capn2
T6584	7730-7734	NCBITaxon_UBERON_EXT:pup	denotes	pups
T6583	7612-7619	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	females
T4774	43-48	PR_EXT:000005015	denotes	Capn2
T4775	58-65	CL:0002322	denotes	ES cell
T4776	61-65	CL_GO_EXT:cell	denotes	cell
T4777	66-72	SO_EXT:sequence_cloned_entity	denotes	clones
T4778	89-94	PR_EXT:000005015	denotes	Capn2
T4779	94-95	SO_EXT:normal_or_wild_type_or_present	denotes	+
T4780	96-97	SO_EXT:sequence_nullness_or_absence	denotes	-
T4781	98-105	CL:0002322	denotes	ES cell
T4782	101-105	CL_GO_EXT:cell	denotes	cell
T4783	174-178	CHEBI_EXT:23888	denotes	drug
T4784	189-195	SO_EXT:sequence_cloned_entity	denotes	clones
T4785	222-227	PR_EXT:000005015	denotes	Capn2
T4786	272-285	GO:0097617	denotes	hybridization
T4787	306-311	CHEBI_SO_EXT:molecular_probe	denotes	probe
T4788	339-347	SO_EXT:sequence_upstreamness	denotes	upstream
T4789	365-375	GO:0097617	denotes	hybridized
T4790	386-387	CHEBI_SO_EXT:base	denotes	b
T4791	388-393	PR_EXT:P23940	denotes	BamHI
T4792	410-419	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T4793	420-426	SO_EXT:0001023	denotes	allele
T4794	436-437	CHEBI_SO_EXT:base	denotes	b
T4795	438-443	PR_EXT:P23940	denotes	BamHI
T4796	460-466	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T4797	467-473	SO_EXT:0001023	denotes	allele
T4798	492-499	SO_EXT:0001026	denotes	genomic
T4799	527-532	CHEBI_SO_EXT:molecular_probe	denotes	probe
T4800	565-566	CHEBI_SO_EXT:base	denotes	b
T4801	567-576	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T4802	586-587	CHEBI_SO_EXT:base	denotes	b
T4803	588-594	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T4804	617-618	CHEBI_SO_EXT:base	denotes	b
T4805	619-628	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T4806	638-639	CHEBI_SO_EXT:base	denotes	b
T4807	640-646	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T4808	679-680	CHEBI_SO_EXT:base	denotes	b
T4809	681-690	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T4810	700-701	CHEBI_SO_EXT:base	denotes	b
T4811	702-708	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T4812	745-750	CHEBI_SO_EXT:molecular_probe	denotes	probe
T4813	768-771	GO_EXT:phosphoglycerate_kinase	denotes	PGK
T4814	772-775	CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase	denotes	Neo
T4815	776-784	SO_EXT:0005853	denotes	cassette
T4816	810-811	CHEBI_SO_EXT:base	denotes	b
T4817	812-817	PR_EXT:P23940	denotes	BamHI
T4818	830-835	PR_EXT:000005015	denotes	Capn2
T4819	835-836	SO_EXT:normal_or_wild_type_or_present	denotes	+
T4820	837-838	SO_EXT:sequence_nullness_or_absence	denotes	-
T4821	839-847	CL:0002322	denotes	ES cells
T4822	842-847	CL_GO_EXT:cell	denotes	cells
T4823	869-885	SO_EXT:0001644	denotes	targeting vector
T4824	915-920	PR_EXT:000005015	denotes	Capn2
T4825	1001-1010	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T4826	1028-1030	SO_EXT:0000028	denotes	bp
T4827	1043-1045	SO_EXT:0000028	denotes	bp
T4828	1063-1069	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T4829	1070-1076	SO_EXT:0001023	denotes	allele
T4830	1088-1090	SO_EXT:0000028	denotes	bp
T4831	1136-1140	CL_GO_EXT:cell	denotes	cell
T6467	2478-2482	NCBITaxon:10088	denotes	mice
T6468	2516-2522	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6469	2523-2528	PR_EXT:000005015	denotes	Capn2
T6470	2529-2535	SO_EXT:0001023	denotes	allele
T6471	2551-2555	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	male
T6472	2556-2560	NCBITaxon:10088	denotes	mice
T6473	2578-2584	UBERON:0000085	denotes	morula
T6474	2619-2624	PR_EXT:000005015	denotes	Capn2
T6475	2624-2625	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6476	2626-2627	SO_EXT:sequence_nullness_or_absence	denotes	-
T6477	2633-2637	CL_GO_EXT:cell	denotes	cell
T6478	2657-2662	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	males
T6479	2679-2684	PR_EXT:000005015	denotes	Capn2
T6480	2686-2687	SO_EXT:sequence_nullness_or_absence	denotes	-
T6481	2693-2701	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T6482	2760-2765	PR_EXT:000005015	denotes	Capn2
T6483	2765-2766	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6484	2767-2768	SO_EXT:sequence_nullness_or_absence	denotes	-
T6485	2769-2776	NCBITaxon:33208	denotes	animals
T6486	2827-2834	UBERON:0001062	denotes	anatomy
T6487	2836-2848	GO:0000003	denotes	reproduction
T6488	2853-2862	UBERON:0000104	denotes	life span
T6489	2920-2925	PR_EXT:000005015	denotes	Capn2
T6490	2925-2926	SO_EXT:sequence_nullness_or_absence	denotes	-
T6491	2927-2928	SO_EXT:sequence_nullness_or_absence	denotes	-
T6492	2995-3004	UBERON:0012101	denotes	perinatal
T6493	2999-3004	GO:0007567	denotes	natal
T6494	3005-3010	GO:0016265	denotes	death
T6495	3019-3024	PR_EXT:000005015	denotes	Capn2
T6496	3024-3025	SO_EXT:sequence_nullness_or_absence	denotes	-
T6497	3026-3027	SO_EXT:sequence_nullness_or_absence	denotes	-
T6498	3074-3079	PR_EXT:000005015	denotes	Capn2
T6499	3079-3080	SO_EXT:sequence_nullness_or_absence	denotes	-
T6500	3081-3082	SO_EXT:sequence_nullness_or_absence	denotes	-
T6501	3083-3090	NCBITaxon:33208	denotes	animals
T6502	3091-3099	GO:0016265	denotes	perished
T6503	3121-3130	UBERON:0000922	denotes	embryonic
T6504	3121-3142	GO:0009790	denotes	embryonic development
T6505	3174-3183	UBERON:0000922	denotes	embryonic
T6506	3184-3189	GO:0016265	denotes	death
T6507	3209-3221	GO:0007566	denotes	implantation
T6508	3229-3236	UBERON:0000922	denotes	embryos
T6509	3314-3319	PR_EXT:000005015	denotes	Capn2
T6510	3319-3320	SO_EXT:sequence_nullness_or_absence	denotes	-
T6511	3321-3322	SO_EXT:sequence_nullness_or_absence	denotes	-
T6512	3323-3330	UBERON:0000922	denotes	embryos
T6513	3361-3367	UBERON:0000922	denotes	embryo
T6514	3428-3433	PR_EXT:000005015	denotes	Capn2
T6515	3433-3434	SO_EXT:sequence_nullness_or_absence	denotes	-
T6516	3435-3436	SO_EXT:sequence_nullness_or_absence	denotes	-
T6517	3437-3444	UBERON:0000922	denotes	embryos
T6518	3454-3459	GO:0016265	denotes	dying
T6519	3469-3481	GO:0007566	denotes	implantation
T6520	3483-3490	UBERON:0000922	denotes	Embryos
T6521	3518-3526	UBERON:0000993	denotes	oviducts
T6522	3530-3538	GO:0007565	denotes	pregnant
T6523	3539-3546	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	females
T6524	3661-3673	GO:0007566	denotes	implantation
T6525	3674-3681	UBERON:0000922	denotes	embryos
T6526	3687-3692	PR_EXT:000005015	denotes	Capn2
T6527	3692-3693	SO_EXT:sequence_nullness_or_absence	denotes	-
T6528	3694-3695	SO_EXT:sequence_nullness_or_absence	denotes	-
T6529	3732-3737	PR_EXT:000005015	denotes	Capn2
T6530	3737-3738	SO_EXT:sequence_nullness_or_absence	denotes	-
T6531	3739-3740	SO_EXT:sequence_nullness_or_absence	denotes	-
T6532	3741-3748	UBERON:0000922	denotes	embryos
T6533	3770-3782	UBERON:0019252	denotes	8-cell stage
T6534	3772-3776	CL_GO_EXT:cell	denotes	cell
T6535	3876-3886	UBERON:0000358	denotes	blastocyst
T6536	3894-3901	UBERON:0000922	denotes	embryos
T6537	3907-3912	PR_EXT:000005015	denotes	Capn2
T6538	3912-3913	SO_EXT:sequence_nullness_or_absence	denotes	-
T6539	3914-3915	SO_EXT:sequence_nullness_or_absence	denotes	-
T6540	3933-3938	PR_EXT:000005015	denotes	Capn2
T6590	7893-7905	GO:0007566	denotes	implantation
T7680	9028-9033	CL_GO_EXT:cell	denotes	cells
T7679	9025-9033	CL:0002322	denotes	ES cells
T7678	9023-9024	SO_EXT:sequence_nullness_or_absence	denotes	-
T7677	9021-9022	SO_EXT:normal_or_wild_type_or_present	denotes	+
T7676	9016-9021	PR_EXT:000005022	denotes	Capn4
T7675	8970-8975	CL_GO_EXT:cell	denotes	cells
T7674	8967-8975	CL:0002322	denotes	ES cells
T7673	8894-8909	GO:0035822	denotes	gene conversion
T7672	8894-8898	SO_EXT:0000704	denotes	gene
T7671	8885-8890	CL_GO_EXT:cell	denotes	cells
T7670	8878-8884	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T7669	8838-8842	CHEBI:42768	denotes	G418
T7668	8794-8800	SO_EXT:sequence_cloned_entity	denotes	clonal
T7667	8770-8775	CL_GO_EXT:cell	denotes	cells
T7666	8767-8775	CL:0002322	denotes	ES cells
T7665	8765-8766	SO_EXT:sequence_nullness_or_absence	denotes	-
T7664	8763-8764	SO_EXT:normal_or_wild_type_or_present	denotes	+
T7663	8758-8763	PR_EXT:000005015	denotes	Capn2
T7662	8752-8757	CL_GO_EXT:cell	denotes	cells
T7661	8749-8757	CL:0002322	denotes	ES cells
T7660	8747-8748	SO_EXT:sequence_nullness_or_absence	denotes	-
T7659	8745-8746	SO_EXT:sequence_nullness_or_absence	denotes	-
T7658	8740-8745	PR_EXT:000005015	denotes	Capn2
T7657	8752-8757	GO:0048468	denotes	cells
T21093	1199-1206	CL:0002322	denotes	ES cell
T21094	1202-1206	CL_GO_EXT:cell	denotes	cell
T21095	1245-1250	PR_EXT:000005015	denotes	Capn2
T21096	1330-1335	PR_EXT:P23940	denotes	BamHI
T21097	1345-1356	SO_EXT:genomic_DNA	denotes	genomic DNA
T21098	1353-1356	CHEBI_SO_EXT:DNA	denotes	DNA
T21099	1372-1380	CL:0002322	denotes	ES cells
T21100	1375-1380	CL_GO_EXT:cell	denotes	cells
T21101	1385-1395	GO:0097617	denotes	hybridized
T21102	1403-1406	CHEBI:42098	denotes	DIG
T21103	1407-1414	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T21104	1419-1421	SO_EXT:0000028	denotes	bp
T21105	1422-1427	PR_EXT:P23940	denotes	BamHI
T21106	1428-1435	PR_EXT:P43870	denotes	HindIII
T21107	1465-1473	SO_EXT:sequence_upstreamness	denotes	upstream
T21108	1498-1514	SO_EXT:0001644	denotes	targeting vector
T21109	1534-1535	CHEBI_SO_EXT:base	denotes	b
T21110	1536-1541	PR_EXT:P23940	denotes	BamHI
T21111	1572-1581	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T21112	1582-1588	SO_EXT:0001023	denotes	allele
T21113	1608-1613	CL_GO_EXT:cell	denotes	cells
T21114	1630-1631	CHEBI_SO_EXT:base	denotes	b
T21115	1650-1656	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T21116	1657-1663	SO_EXT:0001023	denotes	allele
T21117	1693-1697	CL_GO_EXT:cell	denotes	cell
T21118	1829-1831	SO_EXT:0000028	denotes	bp
T21119	1849-1858	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T21120	1859-1865	SO_EXT:0001023	denotes	allele
T21121	1877-1879	SO_EXT:0000028	denotes	bp
T21122	1897-1903	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T21123	1904-1910	SO_EXT:0001023	denotes	allele
T21124	1947-1953	SO_EXT:0000112	denotes	primer
T21125	1965-1971	SO_EXT:0000188	denotes	intron
T21126	2004-2020	SO_EXT:0001644	denotes	targeting vector
T21127	2035-2041	SO_EXT:0001023	denotes	allele
T21128	2051-2060	SO:0000077	denotes	antisense
T21129	2061-2068	SO_EXT:0000112	denotes	primers
T21130	2097-2106	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T21131	2107-2113	SO_EXT:0001023	denotes	allele
T21132	2122-2131	SO:0000077	denotes	antisense
T21133	2132-2138	SO_EXT:0000112	denotes	primer
T21134	2150-2154	SO_EXT:0000147	denotes	exon
T21135	2188-2194	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T21136	2195-2203	SO_EXT:biological_sequence	denotes	sequence
T21137	2221-2230	SO:0000077	denotes	antisense
T21138	2231-2237	SO_EXT:0000112	denotes	primer
T21139	2245-2248	GO_EXT:phosphoglycerate_kinase	denotes	PGK
T21140	2249-2252	CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase	denotes	Neo
T21141	2253-2261	SO_EXT:0005853	denotes	cassette
T21142	2303-2312	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T21143	2313-2319	SO_EXT:0001023	denotes	allele
T21144	2327-2332	CL_GO_EXT:cell	denotes	cells
T21145	2346-2352	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T21146	2353-2359	SO_EXT:0001023	denotes	allele
T21147	2405-2411	SO_EXT:sequence_cloned_entity	denotes	clones
T21148	2414-2415	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	M
T21149	2429-2438	CHEBI_EXT:polyatomic_entity_or_group	denotes	molecular
T21150	2446-2452	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	marker
T22619	6826-6833	UBERON:0000922	denotes	embryos
T22618	6816-6817	SO_EXT:sequence_nullness_or_absence	denotes	-
T22617	6814-6815	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22616	6809-6814	PR_EXT:000005015	denotes	Capn2
T22615	6777-6784	UBERON:0000922	denotes	Embryos
T22614	6768-6775	UBERON:0000922	denotes	embryos
T22613	6751-6761	UBERON:0000358	denotes	blastocyst
T22612	6672-6674	SO_EXT:0000028	denotes	bp
T22611	6656-6662	SO_EXT:0001023	denotes	allele
T22610	6649-6655	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T22609	6638-6640	SO_EXT:0000028	denotes	bp
T22608	6626-6632	SO_EXT:0001023	denotes	allele
T22607	6616-6625	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T22606	6605-6607	SO_EXT:0000028	denotes	bp
T22605	6568-6575	SO_EXT:0001023	denotes	alleles
T22604	6527-6543	SO_EXT:0001644	denotes	targeting vector
T22932	4283-4291	SO_EXT:genotype_or_entity_with_genotype	denotes	Genotype
T22933	4331-4336	PR_EXT:000005015	denotes	Capn2
T22934	4337-4347	SO_EXT:transgenic_entity	denotes	transgenic
T22935	4348-4352	NCBITaxon:10088	denotes	mice
T6551	4225-4230	PR_EXT:000005015	denotes	Capn2
T7656	8726-8739	_FRAGMENT	denotes	generation of
T6541	3949-3956	UBERON:0000922	denotes	embryos
T6542	3974-3986	UBERON:0019252	denotes	8-cell stage
T6543	3976-3980	CL_GO_EXT:cell	denotes	cell
T6544	4014-4023	GO_PR_EXT:calpain_2_complex	denotes	m-calpain
T6545	4038-4045	GO_EXT:fatality_or_lethality	denotes	fatally
T6546	4074-4087	UBERON:0019248	denotes	early embryos
T6547	4161-4165	CHEBI_SO_EXT:mRNA	denotes	mRNA
T6548	4161-4176	CHEBI_SO_EXT:mRNA	denotes	mRNA transcript
T6549	4166-4176	SO_EXT:0000673	denotes	transcript
T6550	4180-4187	CHEBI_PR_EXT:protein	denotes	protein
T6552	4230-4231	SO_EXT:sequence_nullness_or_absence	denotes	-
T6553	4232-4233	SO_EXT:sequence_nullness_or_absence	denotes	-
T6554	4234-4241	UBERON:0000922	denotes	embryos
T6555	4260-4266	UBERON:0000085	denotes	morula
T6582	7590-7595	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	males
T6581	7576-7583	NCBITaxon:33208	denotes	animals
T6580	7574-7575	SO_EXT:sequence_nullness_or_absence	denotes	-
T6579	7572-7573	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6578	7567-7572	PR_EXT:000005015	denotes	Capn2
T6577	7562-7563	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6576	7560-7561	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6575	7555-7560	PR_EXT:000005015	denotes	Capn2
T6574	7519-7526	NCBITaxon:33208	denotes	animals
T6573	7517-7518	SO_EXT:sequence_nullness_or_absence	denotes	-
T6572	7515-7516	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6571	7510-7515	PR_EXT:000005015	denotes	Capn2
T6570	7484-7485	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6569	7482-7483	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6568	7477-7482	PR_EXT:000005015	denotes	Capn2
T6567	7451-7459	GO_EXT:breeding	denotes	breeding
T6566	7417-7424	NCBITaxon:33208	denotes	animals
T6565	7290-7297	NCBITaxon:33208	denotes	animals
T6564	7289-7290	SO_EXT:sequence_nullness_or_absence	denotes	-
T6563	7287-7288	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6562	7282-7287	PR_EXT:000005015	denotes	Capn2
T6561	7277-7278	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6560	7275-7276	SO_EXT:normal_or_wild_type_or_present	denotes	+
T6559	7270-7275	PR_EXT:000005015	denotes	Capn2
T6558	7216-7223	UBERON:0000922	denotes	embryos
T6557	7203-7215	GO:0007566	denotes	implantation
T6556	7181-7193	GO:0007566	denotes	implantation
T6610	8304-8310	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6609	8264-8265	SO_EXT:sequence_nullness_or_absence	denotes	-
T6608	8262-8263	SO_EXT:normal_or_wild_type_or_present	denotes	+
R2220	T7657	T7656	_lexicallyChainedTo	cells,generation of

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