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PMC:1382200 / 39096-39658 JSONTXT

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Id Subject Object Predicate Lexical cue
T20576 421-423 , denotes ,
T20575 418-421 CD denotes 100
T20574 417-418 HYPH denotes -
T20573 409-415 NNP denotes Triton
T20572 416-417 NN denotes X
T20571 407-408 NN denotes %
T20570 404-407 CD denotes 0.5
T20569 402-404 , denotes ,
T20568 398-399 HYPH denotes -
T20567 394-398 NN denotes Tris
T20566 399-402 NN denotes HCl
T20565 391-393 NN denotes mM
T20564 388-390 CD denotes 50
T20563 447-449 NN denotes pH
T20562 387-388 -LRB- denotes (
T20561 380-386 NN denotes buffer
T20560 374-379 NN denotes lysis
T20559 371-373 IN denotes of
T20558 368-370 NN denotes μL
T20557 365-367 CD denotes 20
T20556 362-364 IN denotes in
T20555 359-361 NN denotes °C
T20554 357-359 CD denotes 55
T20553 354-356 IN denotes at
T20552 348-353 NNS denotes hours
T20551 343-347 CD denotes five
T20550 339-342 IN denotes for
T20549 325-329 RB denotes then
T20548 320-324 VBD denotes were
T20547 330-338 VBN denotes digested
T20546 312-319 NNS denotes embryos
T20545 303-311 VBN denotes Isolated
T20544 302-519 sentence denotes Isolated embryos were then digested for five hours at 55°C in 20 μL of lysis buffer (50 mM Tris-HCl, 0.5% Triton X-100, 200 μL/mL proteinase K, pH 8.0), followed by ten minutes at 95°C to inactivate the proteinase K.
T20543 301-302 . denotes .
T20542 290-294 NN denotes cell
T20541 295-301 NN denotes medium
T20540 287-289 NN denotes ES
T20539 282-286 IN denotes with
T20538 273-281 NNS denotes oviducts
T20537 269-272 DT denotes the
T20536 260-268 VBG denotes flushing
T20535 256-259 CC denotes and
T20534 251-255 NN denotes E3.5
T20533 248-250 CC denotes or
T20532 243-247 NN denotes E2.5
T20531 240-242 IN denotes at
T20530 232-239 NNS denotes females
T20529 223-231 JJ denotes pregnant
T20528 220-222 IN denotes of
T20527 216-219 IN denotes out
T20526 210-215 NNS denotes uteri
T20525 206-209 DT denotes the
T20524 195-205 VBG denotes dissecting
T20523 192-194 IN denotes by
T20522 178-182 VBD denotes were
T20521 183-191 VBN denotes obtained
T20520 170-177 NNS denotes embryos
T20519 153-169 JJ denotes Pre-implantation
T20518 152-302 sentence denotes Pre-implantation embryos were obtained by dissecting the uteri out of pregnant females at E2.5 or E3.5 and flushing the oviducts with ES cell medium.
T20517 151-152 . denotes .
T20516 147-151 NN denotes E0.5
T20515 144-146 IN denotes as
T20514 132-135 VBD denotes was
T20513 128-131 NN denotes day
T20512 123-127 DT denotes that
T20511 120-122 IN denotes of
T20510 136-143 VBN denotes defined
T20509 115-119 NN denotes noon
T20508 111-114 CC denotes and
T20507 109-111 , denotes ,
T20506 104-109 NNS denotes plugs
T20505 93-103 NN denotes copulation
T20504 90-92 IN denotes of
T20503 78-89 NN denotes observation
T20502 75-77 IN denotes by
T20501 60-63 VBD denotes was
T20500 46-59 NN denotes fertilization
T20499 43-45 IN denotes of
T20498 64-74 VBN denotes determined
T20497 38-42 NN denotes Time
T20496 37-152 sentence denotes Time of fertilization was determined by observation of copulation plugs, and noon of that day was defined as E0.5.
T20495 30-37 NNS denotes embryos
T20494 13-29 JJ denotes pre-implantation
T20493 10-12 IN denotes of
T20492 0-9 NN denotes Isolation
T20608 561-562 . denotes .
T20607 551-561 NN denotes genotyping
T20606 547-550 NN denotes PCR
T20605 543-546 IN denotes for
T20604 533-537 RB denotes then
T20603 528-532 VBD denotes were
T20602 538-542 VBN denotes used
T20601 520-527 NNS denotes Lysates
T20600 519-562 sentence denotes Lysates were then used for PCR genotyping.
T20599 518-519 . denotes .
T20598 506-516 NN denotes proteinase
T20597 517-518 NN denotes K
T20596 502-505 DT denotes the
T20595 491-501 VB denotes inactivate
T20594 488-490 TO denotes to
T20593 485-487 NN denotes °C
T20592 483-485 CD denotes 95
T20591 480-482 IN denotes at
T20590 472-479 NNS denotes minutes
T20589 468-471 CD denotes ten
T20588 465-467 IN denotes by
T20587 456-464 VBN denotes followed
T20586 454-456 , denotes ,
T20585 453-454 -RRB- denotes )
T20584 450-453 CD denotes 8.0
T20583 445-447 , denotes ,
T20582 433-443 NN denotes proteinase
T20581 430-432 NN denotes mL
T20580 429-430 SYM denotes /
T20579 444-445 NN denotes K
T20578 427-429 NN denotes μL
T20577 423-426 CD denotes 200
R6124 T20493 T20492 prep of,Isolation
R6125 T20494 T20495 amod pre-implantation,embryos
R6126 T20495 T20493 pobj embryos,of
R6127 T20497 T20498 nsubjpass Time,determined
R6128 T20499 T20497 prep of,Time
R6129 T20500 T20499 pobj fertilization,of
R6130 T20501 T20498 auxpass was,determined
R6131 T20502 T20498 agent by,determined
R6132 T20503 T20502 pobj observation,by
R6133 T20504 T20503 prep of,observation
R6134 T20505 T20506 compound copulation,plugs
R6135 T20506 T20504 pobj plugs,of
R6136 T20507 T20498 punct ", ",determined
R6137 T20508 T20498 cc and,determined
R6138 T20509 T20510 nsubjpass noon,defined
R6139 T20510 T20498 conj defined,determined
R6140 T20511 T20509 prep of,noon
R6141 T20512 T20513 det that,day
R6142 T20513 T20511 pobj day,of
R6143 T20514 T20510 auxpass was,defined
R6144 T20515 T20510 prep as,defined
R6145 T20516 T20515 pobj E0.5,as
R6146 T20517 T20510 punct .,defined
R6147 T20519 T20520 amod Pre-implantation,embryos
R6148 T20520 T20521 nsubjpass embryos,obtained
R6149 T20522 T20521 auxpass were,obtained
R6150 T20523 T20521 prep by,obtained
R6151 T20524 T20523 pcomp dissecting,by
R6152 T20525 T20526 det the,uteri
R6153 T20526 T20524 dobj uteri,dissecting
R6154 T20527 T20524 prep out,dissecting
R6155 T20528 T20527 prep of,out
R6156 T20529 T20530 amod pregnant,females
R6157 T20530 T20528 pobj females,of
R6158 T20531 T20524 prep at,dissecting
R6159 T20532 T20531 pobj E2.5,at
R6160 T20533 T20532 cc or,E2.5
R6161 T20534 T20532 conj E3.5,E2.5
R6162 T20535 T20524 cc and,dissecting
R6163 T20536 T20524 conj flushing,dissecting
R6164 T20537 T20538 det the,oviducts
R6165 T20538 T20536 dobj oviducts,flushing
R6166 T20539 T20536 prep with,flushing
R6167 T20540 T20541 compound ES,medium
R6168 T20541 T20539 pobj medium,with
R6169 T20542 T20541 compound cell,medium
R6170 T20543 T20521 punct .,obtained
R6171 T20545 T20546 amod Isolated,embryos
R6172 T20546 T20547 nsubjpass embryos,digested
R6173 T20548 T20547 auxpass were,digested
R6174 T20549 T20547 advmod then,digested
R6175 T20550 T20547 prep for,digested
R6176 T20551 T20552 nummod five,hours
R6177 T20552 T20550 pobj hours,for
R6178 T20553 T20547 prep at,digested
R6179 T20554 T20555 nummod 55,°C
R6180 T20555 T20553 pobj °C,at
R6181 T20556 T20547 prep in,digested
R6182 T20557 T20558 nummod 20,μL
R6183 T20558 T20556 pobj μL,in
R6184 T20559 T20558 prep of,μL
R6185 T20560 T20561 compound lysis,buffer
R6186 T20561 T20559 pobj buffer,of
R6187 T20562 T20563 punct (,pH
R6188 T20563 T20561 parataxis pH,buffer
R6189 T20564 T20565 nummod 50,mM
R6190 T20565 T20566 compound mM,HCl
R6191 T20566 T20563 dep HCl,pH
R6192 T20567 T20566 compound Tris,HCl
R6193 T20568 T20566 punct -,HCl
R6194 T20569 T20563 punct ", ",pH
R6195 T20570 T20571 nummod 0.5,%
R6196 T20571 T20572 compound %,X
R6197 T20572 T20563 dep X,pH
R6198 T20573 T20572 compound Triton,X
R6199 T20574 T20572 punct -,X
R6200 T20575 T20572 nummod 100,X
R6201 T20576 T20563 punct ", ",pH
R6202 T20577 T20578 nummod 200,μL
R6203 T20578 T20579 nmod μL,K
R6204 T20579 T20563 dep K,pH
R6205 T20580 T20581 punct /,mL
R6206 T20581 T20578 prep mL,μL
R6207 T20582 T20579 compound proteinase,K
R6208 T20583 T20563 punct ", ",pH
R6209 T20584 T20563 nummod 8.0,pH
R6210 T20585 T20563 punct ),pH
R6211 T20586 T20561 punct ", ",buffer
R6212 T20587 T20561 acl followed,buffer
R6213 T20588 T20587 agent by,followed
R6214 T20589 T20590 nummod ten,minutes
R6215 T20590 T20588 pobj minutes,by
R6216 T20591 T20590 prep at,minutes
R6217 T20592 T20593 nummod 95,°C
R6218 T20593 T20591 pobj °C,at
R6219 T20594 T20595 aux to,inactivate
R6220 T20595 T20587 advcl inactivate,followed
R6221 T20596 T20597 det the,K
R6222 T20597 T20595 dobj K,inactivate
R6223 T20598 T20597 compound proteinase,K
R6224 T20599 T20547 punct .,digested
R6225 T20601 T20602 nsubjpass Lysates,used
R6226 T20603 T20602 auxpass were,used
R6227 T20604 T20602 advmod then,used
R6228 T20605 T20602 prep for,used
R6229 T20606 T20607 compound PCR,genotyping
R6230 T20607 T20605 pobj genotyping,for
R6231 T20608 T20602 punct .,used

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T20470 409-421 CHEBI:9750 denotes Triton X-100
T20469 399-402 CHEBI:17883 denotes HCl
T20468 394-398 CHEBI:9754 denotes Tris
T20467 374-379 GO:0019835 denotes lysis
T20466 312-319 UBERON:0000922 denotes embryos
T20465 287-294 CL:0002322 denotes ES cell
T20464 273-281 UBERON:0000993 denotes oviducts
T20463 223-231 GO:0007565 denotes pregnant
T20462 210-215 UBERON:0000995 denotes uteri
T20461 170-177 UBERON:0000922 denotes embryos
T20460 157-169 GO:0007566 denotes implantation
T20459 93-109 UBERON:0010148 denotes copulation plugs
T20458 93-103 GO:0007620 denotes copulation
T20457 46-59 GO:0009566 denotes fertilization
T20456 30-37 UBERON:0000922 denotes embryos
T20455 17-29 GO:0007566 denotes implantation

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T20491 506-516 GO_EXT:0008233 denotes proteinase
T20490 433-443 GO_EXT:0008233 denotes proteinase
T20489 409-421 CHEBI:9750 denotes Triton X-100
T20488 399-402 CHEBI:17883 denotes HCl
T20487 394-398 CHEBI:9754 denotes Tris
T20486 380-386 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T20485 374-379 GO:0019835 denotes lysis
T20484 312-319 UBERON:0000922 denotes embryos
T20483 290-294 CL_GO_EXT:cell denotes cell
T20482 287-294 CL:0002322 denotes ES cell
T20481 273-281 UBERON:0000993 denotes oviducts
T20480 232-239 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T20479 223-231 GO:0007565 denotes pregnant
T20478 210-215 UBERON:0000995 denotes uteri
T20477 170-177 UBERON:0000922 denotes embryos
T20476 157-169 GO:0007566 denotes implantation
T20475 93-109 UBERON:0010148 denotes copulation plugs
T20474 93-103 GO:0007620 denotes copulation
T20473 46-59 GO:0009566 denotes fertilization
T20472 30-37 UBERON:0000922 denotes embryos
T20471 17-29 GO:0007566 denotes implantation