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PMC:1382200 / 34640-35224 JSONTXT

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Id Subject Object Predicate Lexical cue
T19647 583-584 . denotes .
T19646 577-583 NNS denotes clones
T19645 568-576 VBN denotes targeted
T19644 565-567 IN denotes in
T19643 547-558 NN denotes integration
T19642 540-546 JJ denotes single
T19641 559-564 NN denotes event
T19640 538-539 DT denotes a
T19639 531-537 VB denotes verify
T19638 528-530 TO denotes to
T19637 522-527 NN denotes order
T19636 519-521 IN denotes in
T19635 513-518 NNS denotes blots
T19634 504-512 NNP denotes Southern
T19633 498-503 VB denotes probe
T19632 495-497 TO denotes to
T19631 485-489 RB denotes also
T19630 481-484 VBD denotes was
T19629 467-468 HYPH denotes -
T19628 468-471 NN denotes Neo
T19627 464-467 NN denotes PGK
T19626 472-480 NN denotes cassette
T19625 460-463 DT denotes the
T19624 455-459 IN denotes from
T19623 490-494 VBN denotes used
T19622 440-441 HYPH denotes -
T19621 441-445 NN denotes XbaI
T19620 436-440 NN denotes PstI
T19619 433-435 NN denotes bp
T19618 429-432 CD denotes 681
T19617 446-454 NN denotes fragment
T19616 427-428 DT denotes A
T19615 426-584 sentence denotes A 681 bp PstI-XbaI fragment from the PGK-Neo cassette was also used to probe Southern blots in order to verify a single integration event in targeted clones.
T19614 425-426 . denotes .
T19613 424-425 -RRB- denotes )
T19612 423-424 CD denotes 1
T19611 416-422 NN denotes Figure
T19610 415-416 -LRB- denotes (
T19609 406-414 NN denotes homology
T19608 403-405 IN denotes of
T19607 393-398 JJ denotes short
T19606 399-402 NN denotes arm
T19605 389-392 DT denotes the
T19604 386-388 IN denotes of
T19603 377-385 RB denotes upstream
T19602 365-376 RB denotes immediately
T19601 357-364 VBN denotes located
T19600 339-340 HYPH denotes -
T19599 340-347 NN denotes HindIII
T19598 334-339 NN denotes BamHI
T19597 322-323 HYPH denotes -
T19596 321-322 CD denotes 4
T19595 323-333 VBG denotes containing
T19594 316-320 NN denotes exon
T19593 313-315 NN denotes bp
T19592 309-312 CD denotes 823
T19591 300-301 HYPH denotes -
T19590 301-308 VBN denotes labeled
T19589 297-300 NN denotes DIG
T19588 348-356 NN denotes fragment
T19587 295-296 DT denotes a
T19586 290-294 IN denotes with
T19585 279-289 VBD denotes hybridized
T19584 275-278 CC denotes and
T19583 263-270 JJ denotes genomic
T19582 271-274 NN denotes DNA
T19581 253-254 HYPH denotes -
T19580 254-262 VBN denotes digested
T19579 248-253 NN denotes BamHI
T19578 243-247 IN denotes with
T19577 230-234 VBD denotes were
T19576 220-229 NNS denotes membranes
T19575 218-220 , denotes ,
T19574 213-218 NNS denotes cases
T19573 208-212 JJS denotes most
T19572 235-242 VBN denotes blotted
T19571 205-207 IN denotes In
T19570 204-426 sentence denotes In most cases, membranes were blotted with BamHI-digested genomic DNA and hybridized with a DIG-labeled 823 bp exon 4-containing BamHI-HindIII fragment located immediately upstream of the short arm of homology (Figure 1).
T19569 203-204 . denotes .
T19568 202-203 -RRB- denotes )
T19567 197-202 NNP denotes Roche
T19566 196-197 -LRB- denotes (
T19565 173-188 JJ denotes non-radioactive
T19564 171-172 -RRB- denotes )
T19563 168-171 NN denotes DIG
T19562 167-168 -LRB- denotes (
T19561 155-166 NN denotes digoxigenin
T19560 189-195 NN denotes system
T19559 151-154 DT denotes the
T19558 145-150 VBG denotes using
T19557 141-144 RP denotes out
T19556 129-132 VBD denotes was
T19555 133-140 VBN denotes carried
T19554 120-128 NN denotes blotting
T19553 111-119 NNP denotes Southern
T19552 110-204 sentence denotes Southern blotting was carried out using the digoxigenin (DIG) non-radioactive system (Roche).
T19551 109-110 . denotes .
T19550 98-103 NN denotes Capn2
T19549 104-109 NN denotes locus
T19548 94-97 DT denotes the
T19547 91-93 IN denotes of
T19546 82-90 NN denotes genotype
T19545 78-81 DT denotes the
T19544 68-77 VB denotes determine
T19543 65-67 TO denotes to
T19542 59-64 NN denotes order
T19541 56-58 IN denotes in
T19540 41-45 VBD denotes were
T19539 30-40 NNS denotes strategies
T19538 26-29 NN denotes PCR
T19537 22-25 CC denotes and
T19536 46-55 VBN denotes exploited
T19535 8-16 NNP denotes Southern
T19534 17-21 NN denotes blot
T19533 0-7 JJ denotes Several
R5470 T19533 T19534 amod Several,blot
R5471 T19534 T19536 nsubjpass blot,exploited
R5472 T19535 T19534 compound Southern,blot
R5473 T19537 T19534 cc and,blot
R5474 T19538 T19539 compound PCR,strategies
R5475 T19539 T19534 conj strategies,blot
R5476 T19540 T19536 auxpass were,exploited
R5477 T19541 T19536 prep in,exploited
R5478 T19542 T19541 pobj order,in
R5479 T19543 T19544 aux to,determine
R5480 T19544 T19542 acl determine,order
R5481 T19545 T19546 det the,genotype
R5482 T19546 T19544 dobj genotype,determine
R5483 T19547 T19546 prep of,genotype
R5484 T19548 T19549 det the,locus
R5485 T19549 T19547 pobj locus,of
R5486 T19550 T19549 compound Capn2,locus
R5487 T19551 T19536 punct .,exploited
R5488 T19553 T19554 compound Southern,blotting
R5489 T19554 T19555 nsubjpass blotting,carried
R5490 T19556 T19555 auxpass was,carried
R5491 T19557 T19555 prt out,carried
R5492 T19558 T19555 advcl using,carried
R5493 T19559 T19560 det the,system
R5494 T19560 T19558 dobj system,using
R5495 T19561 T19560 nmod digoxigenin,system
R5496 T19562 T19561 punct (,digoxigenin
R5497 T19563 T19561 appos DIG,digoxigenin
R5498 T19564 T19560 punct ),system
R5499 T19565 T19560 amod non-radioactive,system
R5500 T19566 T19567 punct (,Roche
R5501 T19567 T19560 parataxis Roche,system
R5502 T19568 T19567 punct ),Roche
R5503 T19569 T19555 punct .,carried
R5504 T19571 T19572 prep In,blotted
R5505 T19573 T19574 amod most,cases
R5506 T19574 T19571 pobj cases,In
R5507 T19575 T19572 punct ", ",blotted
R5508 T19576 T19572 nsubjpass membranes,blotted
R5509 T19577 T19572 auxpass were,blotted
R5510 T19578 T19572 prep with,blotted
R5511 T19579 T19580 npadvmod BamHI,digested
R5512 T19580 T19582 amod digested,DNA
R5513 T19581 T19580 punct -,digested
R5514 T19582 T19578 pobj DNA,with
R5515 T19583 T19582 amod genomic,DNA
R5516 T19584 T19572 cc and,blotted
R5517 T19585 T19572 conj hybridized,blotted
R5518 T19586 T19585 prep with,hybridized
R5519 T19587 T19588 det a,fragment
R5520 T19588 T19586 pobj fragment,with
R5521 T19589 T19590 npadvmod DIG,labeled
R5522 T19590 T19588 amod labeled,fragment
R5523 T19591 T19590 punct -,labeled
R5524 T19592 T19593 nummod 823,bp
R5525 T19593 T19588 nmod bp,fragment
R5526 T19594 T19595 npadvmod exon,containing
R5527 T19595 T19588 amod containing,fragment
R5528 T19596 T19594 nummod 4,exon
R5529 T19597 T19595 punct -,containing
R5530 T19598 T19599 compound BamHI,HindIII
R5531 T19599 T19588 compound HindIII,fragment
R5532 T19600 T19599 punct -,HindIII
R5533 T19601 T19588 acl located,fragment
R5534 T19602 T19603 advmod immediately,upstream
R5535 T19603 T19601 advmod upstream,located
R5536 T19604 T19603 prep of,upstream
R5537 T19605 T19606 det the,arm
R5538 T19606 T19604 pobj arm,of
R5539 T19607 T19606 amod short,arm
R5540 T19608 T19606 prep of,arm
R5541 T19609 T19608 pobj homology,of
R5542 T19610 T19611 punct (,Figure
R5543 T19611 T19585 parataxis Figure,hybridized
R5544 T19612 T19611 nummod 1,Figure
R5545 T19613 T19611 punct ),Figure
R5546 T19614 T19572 punct .,blotted
R5547 T19616 T19617 det A,fragment
R5548 T19617 T19623 nsubjpass fragment,used
R5549 T19618 T19619 nummod 681,bp
R5550 T19619 T19617 compound bp,fragment
R5551 T19620 T19621 compound PstI,XbaI
R5552 T19621 T19617 compound XbaI,fragment
R5553 T19622 T19621 punct -,XbaI
R5554 T19624 T19617 prep from,fragment
R5555 T19625 T19626 det the,cassette
R5556 T19626 T19624 pobj cassette,from
R5557 T19627 T19628 compound PGK,Neo
R5558 T19628 T19626 compound Neo,cassette
R5559 T19629 T19628 punct -,Neo
R5560 T19630 T19623 auxpass was,used
R5561 T19631 T19623 advmod also,used
R5562 T19632 T19633 aux to,probe
R5563 T19633 T19623 advcl probe,used
R5564 T19634 T19635 compound Southern,blots
R5565 T19635 T19633 dobj blots,probe
R5566 T19636 T19623 prep in,used
R5567 T19637 T19636 pobj order,in
R5568 T19638 T19639 aux to,verify
R5569 T19639 T19637 acl verify,order
R5570 T19640 T19641 det a,event
R5571 T19641 T19639 dobj event,verify
R5572 T19642 T19641 amod single,event
R5573 T19643 T19641 compound integration,event
R5574 T19644 T19623 prep in,used
R5575 T19645 T19646 amod targeted,clones
R5576 T19646 T19644 pobj clones,in
R5577 T19647 T19623 punct .,used

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T19348 472-480 SO:0005853 denotes cassette
T19347 433-435 SO:0000028 denotes bp
T19346 340-347 PR:P43870 denotes HindIII
T19345 334-339 PR:P23940 denotes BamHI
T19344 316-320 SO:0000147 denotes exon
T19343 313-315 SO:0000028 denotes bp
T19342 297-300 CHEBI:42098 denotes DIG
T19341 279-289 GO:0097617 denotes hybridized
T19340 263-270 SO:0001026 denotes genomic
T19339 248-253 PR:P23940 denotes BamHI
T19338 168-171 CHEBI:42098 denotes DIG
T19337 155-166 CHEBI:42098 denotes digoxigenin
T19336 98-103 PR:000005015 denotes Capn2

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T19415 98-103 PR_EXT:000005015 denotes Capn2
T19433 577-583 SO_EXT:sequence_cloned_entity denotes clones
T19432 472-480 SO_EXT:0005853 denotes cassette
T19431 468-471 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T19430 464-467 GO_EXT:phosphoglycerate_kinase denotes PGK
T19429 433-435 SO_EXT:0000028 denotes bp
T19428 377-385 SO_EXT:sequence_upstreamness denotes upstream
T19427 340-347 PR_EXT:P43870 denotes HindIII
T19426 334-339 PR_EXT:P23940 denotes BamHI
T19425 316-320 SO_EXT:0000147 denotes exon
T19424 313-315 SO_EXT:0000028 denotes bp
T19423 301-308 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T19422 297-300 CHEBI:42098 denotes DIG
T19421 279-289 GO:0097617 denotes hybridized
T19420 271-274 CHEBI_SO_EXT:DNA denotes DNA
T19419 263-274 SO_EXT:genomic_DNA denotes genomic DNA
T19418 248-253 PR_EXT:P23940 denotes BamHI
T19417 168-171 CHEBI:42098 denotes DIG
T19416 155-166 CHEBI:42098 denotes digoxigenin
T19414 82-90 SO_EXT:genotype_or_entity_with_genotype denotes genotype