Id |
Subject |
Object |
Predicate |
Lexical cue |
T17710 |
427-428 |
-RRB- |
denotes |
) |
T17709 |
422-427 |
RB |
denotes |
below |
T17708 |
418-421 |
VB |
denotes |
see |
T17707 |
417-418 |
-LRB- |
denotes |
( |
T17706 |
408-416 |
NN |
denotes |
analysis |
T17705 |
404-407 |
NN |
denotes |
PCR |
T17704 |
400-403 |
CC |
denotes |
and |
T17703 |
391-399 |
NN |
denotes |
blotting |
T17702 |
382-390 |
NNP |
denotes |
Southern |
T17701 |
379-381 |
IN |
denotes |
by |
T17700 |
369-378 |
VBN |
denotes |
genotyped |
T17699 |
365-368 |
CC |
denotes |
and |
T17698 |
363-365 |
, |
denotes |
, |
T17697 |
357-363 |
NNS |
denotes |
plates |
T17696 |
349-350 |
HYPH |
denotes |
- |
T17695 |
350-356 |
VBN |
denotes |
coated |
T17694 |
342-349 |
NN |
denotes |
gelatin |
T17693 |
339-341 |
IN |
denotes |
on |
T17692 |
330-338 |
VBN |
denotes |
expanded |
T17691 |
328-330 |
, |
denotes |
, |
T17690 |
317-321 |
VBD |
denotes |
were |
T17689 |
322-328 |
VBN |
denotes |
picked |
T17688 |
310-316 |
NNS |
denotes |
clones |
T17687 |
299-300 |
HYPH |
denotes |
- |
T17686 |
300-309 |
JJ |
denotes |
resistant |
T17685 |
295-299 |
NN |
denotes |
Drug |
T17684 |
294-429 |
sentence |
denotes |
Drug-resistant clones were picked, expanded on gelatin-coated plates, and genotyped by Southern blotting and PCR analysis (see below). |
T17683 |
293-294 |
. |
denotes |
. |
T17682 |
289-293 |
NNS |
denotes |
days |
T17681 |
283-288 |
CD |
denotes |
eight |
T17680 |
279-282 |
IN |
denotes |
for |
T17679 |
277-278 |
-RRB- |
denotes |
) |
T17678 |
273-277 |
NNP |
denotes |
Inc. |
T17677 |
271-273 |
, |
denotes |
, |
T17676 |
265-271 |
NNP |
denotes |
Syntex |
T17675 |
264-265 |
-LRB- |
denotes |
( |
T17674 |
252-263 |
NN |
denotes |
ganciclovir |
T17673 |
249-251 |
NN |
denotes |
μM |
T17672 |
247-248 |
CD |
denotes |
2 |
T17671 |
243-246 |
CC |
denotes |
and |
T17670 |
241-242 |
-RRB- |
denotes |
) |
T17669 |
236-237 |
HYPH |
denotes |
- |
T17668 |
231-236 |
NNP |
denotes |
Gibco |
T17667 |
238-241 |
NNP |
denotes |
BRL |
T17666 |
230-231 |
-LRB- |
denotes |
( |
T17665 |
222-224 |
NN |
denotes |
ml |
T17664 |
221-222 |
SYM |
denotes |
/ |
T17663 |
225-229 |
NN |
denotes |
G418 |
T17662 |
219-221 |
NN |
denotes |
μg |
T17661 |
215-218 |
CD |
denotes |
200 |
T17660 |
212-214 |
IN |
denotes |
of |
T17659 |
203-211 |
NN |
denotes |
presence |
T17658 |
199-202 |
DT |
denotes |
the |
T17657 |
196-198 |
IN |
denotes |
in |
T17656 |
182-186 |
VBD |
denotes |
were |
T17655 |
187-195 |
VBN |
denotes |
selected |
T17654 |
175-181 |
NNS |
denotes |
clones |
T17653 |
163-174 |
VBN |
denotes |
transformed |
T17652 |
159-162 |
CC |
denotes |
and |
T17651 |
152-158 |
NNS |
denotes |
plates |
T17650 |
144-145 |
HYPH |
denotes |
- |
T17649 |
145-151 |
VBN |
denotes |
coated |
T17648 |
137-144 |
NN |
denotes |
gelatin |
T17647 |
134-136 |
IN |
denotes |
on |
T17646 |
127-133 |
NNS |
denotes |
layers |
T17645 |
120-126 |
NN |
denotes |
feeder |
T17644 |
112-119 |
IN |
denotes |
without |
T17643 |
100-104 |
VBD |
denotes |
were |
T17642 |
105-111 |
VBN |
denotes |
plated |
T17641 |
94-99 |
NNS |
denotes |
Cells |
T17640 |
93-294 |
sentence |
denotes |
Cells were plated without feeder layers on gelatin-coated plates and transformed clones were selected in the presence of 200 μg/ml G418 (Gibco- BRL) and 2 μM ganciclovir (Syntex, Inc.) for eight days. |
T17639 |
92-93 |
. |
denotes |
. |
T17638 |
84-86 |
NN |
denotes |
ES |
T17637 |
87-92 |
NNS |
denotes |
cells |
T17636 |
81-83 |
NN |
denotes |
R1 |
T17635 |
76-80 |
IN |
denotes |
into |
T17634 |
61-75 |
VBN |
denotes |
electroporated |
T17633 |
57-60 |
CC |
denotes |
and |
T17632 |
47-56 |
NN |
denotes |
digestion |
T17631 |
42-46 |
NN |
denotes |
NotI |
T17630 |
39-41 |
IN |
denotes |
by |
T17629 |
24-27 |
VBD |
denotes |
was |
T17628 |
28-38 |
VBN |
denotes |
linearized |
T17627 |
4-13 |
NN |
denotes |
targeting |
T17626 |
14-23 |
NN |
denotes |
construct |
T17625 |
0-3 |
DT |
denotes |
The |
T17711 |
428-429 |
. |
denotes |
. |
R5275 |
T17625 |
T17626 |
det |
The,construct |
R5276 |
T17626 |
T17628 |
nsubjpass |
construct,linearized |
R5277 |
T17627 |
T17626 |
compound |
targeting,construct |
R5278 |
T17629 |
T17628 |
auxpass |
was,linearized |
R5279 |
T17630 |
T17628 |
agent |
by,linearized |
R5280 |
T17631 |
T17632 |
compound |
NotI,digestion |
R5281 |
T17632 |
T17630 |
pobj |
digestion,by |
R5282 |
T17633 |
T17628 |
cc |
and,linearized |
R5283 |
T17634 |
T17628 |
conj |
electroporated,linearized |
R5284 |
T17635 |
T17634 |
prep |
into,electroporated |
R5285 |
T17636 |
T17637 |
compound |
R1,cells |
R5286 |
T17637 |
T17635 |
pobj |
cells,into |
R5287 |
T17638 |
T17637 |
compound |
ES,cells |
R5288 |
T17639 |
T17628 |
punct |
.,linearized |
R5289 |
T17641 |
T17642 |
nsubjpass |
Cells,plated |
R5290 |
T17643 |
T17642 |
auxpass |
were,plated |
R5291 |
T17644 |
T17642 |
prep |
without,plated |
R5292 |
T17645 |
T17646 |
compound |
feeder,layers |
R5293 |
T17646 |
T17644 |
pobj |
layers,without |
R5294 |
T17647 |
T17642 |
prep |
on,plated |
R5295 |
T17648 |
T17649 |
npadvmod |
gelatin,coated |
R5296 |
T17649 |
T17651 |
amod |
coated,plates |
R5297 |
T17650 |
T17649 |
punct |
-,coated |
R5298 |
T17651 |
T17647 |
pobj |
plates,on |
R5299 |
T17652 |
T17642 |
cc |
and,plated |
R5300 |
T17653 |
T17654 |
amod |
transformed,clones |
R5301 |
T17654 |
T17655 |
nsubjpass |
clones,selected |
R5302 |
T17655 |
T17642 |
conj |
selected,plated |
R5303 |
T17656 |
T17655 |
auxpass |
were,selected |
R5304 |
T17657 |
T17655 |
prep |
in,selected |
R5305 |
T17658 |
T17659 |
det |
the,presence |
R5306 |
T17659 |
T17657 |
pobj |
presence,in |
R5307 |
T17660 |
T17659 |
prep |
of,presence |
R5308 |
T17661 |
T17662 |
nummod |
200,μg |
R5309 |
T17662 |
T17663 |
nmod |
μg,G418 |
R5310 |
T17663 |
T17660 |
pobj |
G418,of |
R5311 |
T17664 |
T17665 |
punct |
/,ml |
R5312 |
T17665 |
T17662 |
prep |
ml,μg |
R5313 |
T17666 |
T17667 |
punct |
(,BRL |
R5314 |
T17667 |
T17663 |
parataxis |
BRL,G418 |
R5315 |
T17668 |
T17667 |
compound |
Gibco,BRL |
R5316 |
T17669 |
T17667 |
punct |
-,BRL |
R5317 |
T17670 |
T17667 |
punct |
),BRL |
R5318 |
T17671 |
T17663 |
cc |
and,G418 |
R5319 |
T17672 |
T17673 |
nummod |
2,μM |
R5320 |
T17673 |
T17674 |
compound |
μM,ganciclovir |
R5321 |
T17674 |
T17663 |
conj |
ganciclovir,G418 |
R5322 |
T17675 |
T17676 |
punct |
(,Syntex |
R5323 |
T17676 |
T17674 |
parataxis |
Syntex,ganciclovir |
R5324 |
T17677 |
T17676 |
punct |
", ",Syntex |
R5325 |
T17678 |
T17676 |
amod |
Inc.,Syntex |
R5326 |
T17679 |
T17676 |
punct |
),Syntex |
R5327 |
T17680 |
T17655 |
prep |
for,selected |
R5328 |
T17681 |
T17682 |
nummod |
eight,days |
R5329 |
T17682 |
T17680 |
pobj |
days,for |
R5330 |
T17683 |
T17655 |
punct |
.,selected |
R5331 |
T17685 |
T17686 |
npadvmod |
Drug,resistant |
R5332 |
T17686 |
T17688 |
amod |
resistant,clones |
R5333 |
T17687 |
T17686 |
punct |
-,resistant |
R5334 |
T17688 |
T17689 |
nsubjpass |
clones,picked |
R5335 |
T17690 |
T17689 |
auxpass |
were,picked |
R5336 |
T17691 |
T17689 |
punct |
", ",picked |
R5337 |
T17692 |
T17689 |
conj |
expanded,picked |
R5338 |
T17693 |
T17692 |
prep |
on,expanded |
R5339 |
T17694 |
T17695 |
npadvmod |
gelatin,coated |
R5340 |
T17695 |
T17697 |
amod |
coated,plates |
R5341 |
T17696 |
T17695 |
punct |
-,coated |
R5342 |
T17697 |
T17693 |
pobj |
plates,on |
R5343 |
T17698 |
T17692 |
punct |
", ",expanded |
R5344 |
T17699 |
T17692 |
cc |
and,expanded |
R5345 |
T17700 |
T17692 |
conj |
genotyped,expanded |
R5346 |
T17701 |
T17700 |
prep |
by,genotyped |
R5347 |
T17702 |
T17703 |
compound |
Southern,blotting |
R5348 |
T17703 |
T17701 |
pobj |
blotting,by |
R5349 |
T17704 |
T17703 |
cc |
and,blotting |
R5350 |
T17705 |
T17706 |
compound |
PCR,analysis |
R5351 |
T17706 |
T17703 |
conj |
analysis,blotting |
R5352 |
T17707 |
T17708 |
punct |
(,see |
R5353 |
T17708 |
T17700 |
parataxis |
see,genotyped |
R5354 |
T17709 |
T17708 |
advmod |
below,see |
R5355 |
T17710 |
T17708 |
punct |
),see |
R5356 |
T17711 |
T17689 |
punct |
.,picked |