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Id Subject Object Predicate Lexical cue
T3244 11-14 DT denotes The
T3245 55-64 NNS denotes proteases
T3246 15-18 CD denotes two
T3247 19-29 JJ denotes ubiquitous
T3248 30-33 NN denotes Ca2
T3249 35-44 JJ denotes dependent
T3250 33-34 SYM denotes +
T3251 34-35 HYPH denotes -
T3252 44-46 , denotes ,
T3253 46-54 NN denotes cysteine
T3254 122-125 VBP denotes are
T3255 65-70 VBN denotes known
T3256 71-73 IN denotes as
T3257 74-75 NN denotes μ
T3258 76-83 NN denotes calpain
T3259 75-76 HYPH denotes -
T3260 84-85 -LRB- denotes (
T3261 85-92 NN denotes calpain
T3262 92-93 HYPH denotes -
T3263 93-94 CD denotes 1
T3264 94-95 -RRB- denotes )
T3265 96-99 CC denotes and
T3266 100-101 NN denotes m
T3267 102-109 NN denotes calpain
T3268 101-102 HYPH denotes -
T3269 110-111 -LRB- denotes (
T3270 111-117 NN denotes capain
T3271 117-118 HYPH denotes -
T3272 118-119 CD denotes 2
T3273 119-120 -RRB- denotes )
T3274 120-122 , denotes ,
T3275 126-129 DT denotes the
T3276 139-146 NNS denotes members
T3277 130-138 NN denotes founding
T3278 147-149 IN denotes of
T3279 150-151 DT denotes a
T3280 157-163 NN denotes family
T3281 152-156 NN denotes gene
T3282 164-174 VBG denotes comprising
T3283 175-177 CD denotes 13
T3284 178-183 NNS denotes genes
T3285 184-186 IN denotes in
T3286 187-194 NNS denotes mammals
T3287 195-196 -LRB- denotes [
T3288 196-197 CD denotes 1
T3289 197-198 SYM denotes -
T3290 198-199 CD denotes 3
T3291 199-200 -RRB- denotes ]
T3292 200-201 . denotes .
T3293 201-434 sentence denotes Both are heterodimeric enzymes consisting of distinct 80 kDa catalytic subunits, encoded by the Capn1 (μ-80 k) and Capn2 (m-80 k) genes, respectively, that associate with a common 28 kDa regulatory subunit encoded by the Capn4 gene.
T3294 202-206 DT denotes Both
T3295 207-210 VBP denotes are
T3296 211-224 JJ denotes heterodimeric
T3297 225-232 NNS denotes enzymes
T3298 233-243 VBG denotes consisting
T3299 244-246 IN denotes of
T3300 247-255 JJ denotes distinct
T3301 273-281 NNS denotes subunits
T3302 256-258 CD denotes 80
T3303 259-262 NN denotes kDa
T3304 263-272 JJ denotes catalytic
T3305 281-283 , denotes ,
T3306 283-290 VBN denotes encoded
T3307 291-293 IN denotes by
T3308 294-297 DT denotes the
T3309 332-337 NNS denotes genes
T3310 298-303 NN denotes Capn1
T3311 304-305 -LRB- denotes (
T3312 310-311 NN denotes k
T3313 305-306 NN denotes μ
T3314 306-307 HYPH denotes -
T3315 307-309 CD denotes 80
T3316 311-312 -RRB- denotes )
T3317 313-316 CC denotes and
T3318 317-322 NN denotes Capn2
T3319 323-324 -LRB- denotes (
T3320 329-330 NN denotes k
T3321 324-325 NN denotes m
T3322 325-326 HYPH denotes -
T3323 326-328 CD denotes 80
T3324 330-331 -RRB- denotes )
T3325 337-339 , denotes ,
T3326 339-351 RB denotes respectively
T3327 351-353 , denotes ,
T3328 353-357 WDT denotes that
T3329 358-367 VBP denotes associate
T3330 368-372 IN denotes with
T3331 373-374 DT denotes a
T3332 400-407 NN denotes subunit
T3333 375-381 JJ denotes common
T3334 382-384 CD denotes 28
T3335 385-388 NN denotes kDa
T3336 389-399 JJ denotes regulatory
T3337 408-415 VBN denotes encoded
T3338 416-418 IN denotes by
T3339 419-422 DT denotes the
T3340 429-433 NN denotes gene
T3341 423-428 NN denotes Capn4
T3342 433-434 . denotes .
T3343 434-604 sentence denotes The μ-80 k and m-80 k subunits share 62% amino acid sequence identity, and are very similar in terms of structure, protein chemistry, and in vitro substrate specificity.
T3344 435-438 DT denotes The
T3345 457-465 NNS denotes subunits
T3346 439-440 NN denotes μ
T3347 444-445 NN denotes k
T3348 440-441 HYPH denotes -
T3349 441-443 CD denotes 80
T3350 446-449 CC denotes and
T3351 450-451 NN denotes m
T3352 455-456 NN denotes k
T3353 451-452 HYPH denotes -
T3354 452-454 CD denotes 80
T3355 466-471 VBP denotes share
T3356 472-474 CD denotes 62
T3357 474-475 NN denotes %
T3358 496-504 NN denotes identity
T3359 476-481 NN denotes amino
T3360 482-486 NN denotes acid
T3361 487-495 NN denotes sequence
T3362 504-506 , denotes ,
T3363 506-509 CC denotes and
T3364 510-513 VBP denotes are
T3365 514-518 RB denotes very
T3366 519-526 JJ denotes similar
T3367 527-529 IN denotes in
T3368 530-535 NNS denotes terms
T3369 536-538 IN denotes of
T3370 539-548 NN denotes structure
T3371 548-550 , denotes ,
T3372 550-557 NN denotes protein
T3373 558-567 NN denotes chemistry
T3374 567-569 , denotes ,
T3375 569-572 CC denotes and
T3376 573-575 FW denotes in
T3377 576-581 FW denotes vitro
T3378 592-603 NN denotes specificity
T3379 582-591 NN denotes substrate
T3380 603-604 . denotes .
T3381 604-774 sentence denotes Despite these similarities, the differential expression patterns of μ- and m-calpain in mammalian tissues suggest they have some isoform specific and distinct functions.
T3382 605-612 IN denotes Despite
T3383 711-718 VBP denotes suggest
T3384 613-618 DT denotes these
T3385 619-631 NNS denotes similarities
T3386 631-633 , denotes ,
T3387 633-636 DT denotes the
T3388 661-669 NNS denotes patterns
T3389 637-649 JJ denotes differential
T3390 650-660 NN denotes expression
T3391 670-672 IN denotes of
T3392 673-674 NN denotes μ
T3393 682-689 NN denotes calpain
T3394 674-675 HYPH denotes -
T3395 676-679 CC denotes and
T3396 680-681 NN denotes m
T3397 681-682 HYPH denotes -
T3398 690-692 IN denotes in
T3399 693-702 JJ denotes mammalian
T3400 703-710 NNS denotes tissues
T3401 719-723 PRP denotes they
T3402 724-728 VBP denotes have
T3403 729-733 DT denotes some
T3404 764-773 NNS denotes functions
T3405 734-741 NN denotes isoform
T3406 742-750 JJ denotes specific
T3407 751-754 CC denotes and
T3408 755-763 JJ denotes distinct
T3409 773-774 . denotes .
T3410 774-936 sentence denotes The μ and m designations derive from the levels of Ca2+ required in vitro for optimal activation; 10–50 μM Ca2+ for μ-calpain and 0.3–0.35 mM Ca2+ for m-calpain.
T3411 775-778 DT denotes The
T3412 787-799 NNS denotes designations
T3413 779-780 NN denotes μ
T3414 781-784 CC denotes and
T3415 785-786 NN denotes m
T3416 800-806 VBP denotes derive
T3417 807-811 IN denotes from
T3418 812-815 DT denotes the
T3419 816-822 NNS denotes levels
T3420 823-825 IN denotes of
T3421 826-829 NN denotes Ca2
T3422 829-830 SYM denotes +
T3423 831-839 VBN denotes required
T3424 840-842 FW denotes in
T3425 843-848 FW denotes vitro
T3426 849-852 IN denotes for
T3427 853-860 JJ denotes optimal
T3428 861-871 NN denotes activation
T3429 871-872 : denotes ;
T3430 873-875 CD denotes 10
T3431 876-878 CD denotes 50
T3432 875-876 SYM denotes –
T3433 879-881 NN denotes μM
T3434 882-885 NN denotes Ca2
T3435 885-886 SYM denotes +
T3436 887-890 IN denotes for
T3437 891-892 NN denotes μ
T3438 893-900 NN denotes calpain
T3439 892-893 HYPH denotes -
T3440 901-904 CC denotes and
T3441 905-908 CD denotes 0.3
T3442 909-913 CD denotes 0.35
T3443 908-909 SYM denotes –
T3444 914-916 NN denotes mM
T3445 917-920 NN denotes Ca2
T3446 920-921 SYM denotes +
T3447 922-925 IN denotes for
T3448 926-927 NN denotes m
T3449 928-935 NN denotes calpain
T3450 927-928 HYPH denotes -
T3451 935-936 . denotes .
T3452 936-1098 sentence denotes It is generally assumed that μ- and m-calpain maintain their differential sensitivities to calcium in vivo, although this has not yet been strictly demonstrated.
T3453 937-939 PRP denotes It
T3454 953-960 VBN denotes assumed
T3455 940-942 VBZ denotes is
T3456 943-952 RB denotes generally
T3457 961-965 IN denotes that
T3458 983-991 VBP denotes maintain
T3459 966-967 NN denotes μ
T3460 975-982 NN denotes calpain
T3461 967-968 HYPH denotes -
T3462 969-972 CC denotes and
T3463 973-974 NN denotes m
T3464 974-975 HYPH denotes -
T3465 992-997 PRP$ denotes their
T3466 1011-1024 NNS denotes sensitivities
T3467 998-1010 JJ denotes differential
T3468 1025-1027 IN denotes to
T3469 1028-1035 NN denotes calcium
T3470 1036-1038 FW denotes in
T3471 1039-1043 FW denotes vivo
T3472 1043-1045 , denotes ,
T3473 1045-1053 IN denotes although
T3474 1085-1097 VBN denotes demonstrated
T3475 1054-1058 DT denotes this
T3476 1059-1062 VBZ denotes has
T3477 1063-1066 RB denotes not
T3478 1067-1070 RB denotes yet
T3479 1071-1075 VBN denotes been
T3480 1076-1084 RB denotes strictly
T3481 1097-1098 . denotes .
T3482 1098-1284 sentence denotes Furthermore, since the cytoplasmic free Ca2+ concentration is typically less than 1 μM, it is also assumed that other in vivo factors must contribute to regulation of these enzymes [3].
T3483 1099-1110 RB denotes Furthermore
T3484 1198-1205 VBN denotes assumed
T3485 1110-1112 , denotes ,
T3486 1112-1117 IN denotes since
T3487 1158-1160 VBZ denotes is
T3488 1118-1121 DT denotes the
T3489 1144-1157 NN denotes concentration
T3490 1122-1133 JJ denotes cytoplasmic
T3491 1134-1138 JJ denotes free
T3492 1139-1142 NN denotes Ca2
T3493 1142-1143 SYM denotes +
T3494 1161-1170 RB denotes typically
T3495 1171-1175 JJR denotes less
T3496 1181-1182 CD denotes 1
T3497 1176-1180 IN denotes than
T3498 1183-1185 NN denotes μM
T3499 1185-1187 , denotes ,
T3500 1187-1189 PRP denotes it
T3501 1190-1192 VBZ denotes is
T3502 1193-1197 RB denotes also
T3503 1206-1210 IN denotes that
T3504 1238-1248 VB denotes contribute
T3505 1211-1216 JJ denotes other
T3506 1225-1232 NNS denotes factors
T3507 1217-1219 FW denotes in
T3508 1220-1224 FW denotes vivo
T3509 1233-1237 MD denotes must
T3510 1249-1251 IN denotes to
T3511 1252-1262 NN denotes regulation
T3512 1263-1265 IN denotes of
T3513 1266-1271 DT denotes these
T3514 1272-1279 NNS denotes enzymes
T3515 1280-1281 -LRB- denotes [
T3516 1281-1282 CD denotes 3
T3517 1282-1283 -RRB- denotes ]
T3518 1283-1284 . denotes .
T3519 1284-1454 sentence denotes Without apriori knowledge of the factors regulating calpain activity or their relevant substrates, elucidation of biological functions for calpains presents a challenge.
T3520 1285-1292 IN denotes Without
T3521 1433-1441 VBZ denotes presents
T3522 1293-1300 FW denotes apriori
T3523 1301-1310 NN denotes knowledge
T3524 1311-1313 IN denotes of
T3525 1314-1317 DT denotes the
T3526 1318-1325 NNS denotes factors
T3527 1326-1336 VBG denotes regulating
T3528 1337-1344 NN denotes calpain
T3529 1345-1353 NN denotes activity
T3530 1354-1356 CC denotes or
T3531 1357-1362 PRP$ denotes their
T3532 1372-1382 NNS denotes substrates
T3533 1363-1371 JJ denotes relevant
T3534 1382-1384 , denotes ,
T3535 1384-1395 NN denotes elucidation
T3536 1396-1398 IN denotes of
T3537 1399-1409 JJ denotes biological
T3538 1410-1419 NNS denotes functions
T3539 1420-1423 IN denotes for
T3540 1424-1432 NNS denotes calpains
T3541 1442-1443 DT denotes a
T3542 1444-1453 NN denotes challenge
T3543 1453-1454 . denotes .
T3544 1454-1869 sentence denotes Research on calpains has linked them with a wide variety of functions including muscle growth, development, degeneration (3), neuronal growth and neurodegeneration [4], cell cycle progression [5,6], signal cascades triggered by integrins and growth factors [7], membrane protrusion [8], remodeling of the cytoskeleton and cell migration [9-15], and regulation of cell death via both necrosis and apoptosis [16-22].
T3545 1455-1463 NN denotes Research
T3546 1480-1486 VBN denotes linked
T3547 1464-1466 IN denotes on
T3548 1467-1475 NNS denotes calpains
T3549 1476-1479 VBZ denotes has
T3550 1487-1491 PRP denotes them
T3551 1492-1496 IN denotes with
T3552 1497-1498 DT denotes a
T3553 1504-1511 NN denotes variety
T3554 1499-1503 JJ denotes wide
T3555 1512-1514 IN denotes of
T3556 1515-1524 NNS denotes functions
T3557 1525-1534 VBG denotes including
T3558 1535-1541 NN denotes muscle
T3559 1542-1548 NN denotes growth
T3560 1548-1550 , denotes ,
T3561 1550-1561 NN denotes development
T3562 1561-1563 , denotes ,
T3563 1563-1575 NN denotes degeneration
T3564 1576-1577 -LRB- denotes (
T3565 1577-1578 CD denotes 3
T3566 1578-1579 -RRB- denotes )
T3567 1579-1581 , denotes ,
T3568 1581-1589 JJ denotes neuronal
T3569 1590-1596 NN denotes growth
T3570 1597-1600 CC denotes and
T3571 1601-1618 NN denotes neurodegeneration
T3572 1619-1620 -LRB- denotes [
T3573 1620-1621 CD denotes 4
T3574 1621-1622 -RRB- denotes ]
T3575 1622-1624 , denotes ,
T3576 1624-1628 NN denotes cell
T3577 1629-1634 NN denotes cycle
T3578 1635-1646 NN denotes progression
T3579 1647-1648 -LRB- denotes [
T3580 1650-1651 CD denotes 6
T3581 1648-1649 CD denotes 5
T3582 1649-1650 , denotes ,
T3583 1651-1652 -RRB- denotes ]
T3584 1652-1654 , denotes ,
T3585 1654-1660 NN denotes signal
T3586 1661-1669 NNS denotes cascades
T3587 1670-1679 VBN denotes triggered
T3588 1680-1682 IN denotes by
T3589 1683-1692 NNS denotes integrins
T3590 1693-1696 CC denotes and
T3591 1697-1703 NN denotes growth
T3592 1704-1711 NNS denotes factors
T3593 1712-1713 -LRB- denotes [
T3594 1713-1714 CD denotes 7
T3595 1714-1715 -RRB- denotes ]
T3596 1715-1717 , denotes ,
T3597 1717-1725 NN denotes membrane
T3598 1726-1736 NN denotes protrusion
T3599 1737-1738 -LRB- denotes [
T3600 1738-1739 CD denotes 8
T3601 1739-1740 -RRB- denotes ]
T3602 1740-1742 , denotes ,
T3603 1742-1752 VBG denotes remodeling
T3604 1753-1755 IN denotes of
T3605 1756-1759 DT denotes the
T3606 1760-1772 NN denotes cytoskeleton
T3607 1773-1776 CC denotes and
T3608 1777-1781 NN denotes cell
T3609 1782-1791 NN denotes migration
T3610 1792-1793 -LRB- denotes [
T3611 1793-1794 CD denotes 9
T3612 1794-1795 SYM denotes -
T3613 1795-1797 CD denotes 15
T3614 1797-1798 -RRB- denotes ]
T3615 1798-1800 , denotes ,
T3616 1800-1803 CC denotes and
T3617 1804-1814 NN denotes regulation
T3618 1815-1817 IN denotes of
T3619 1818-1822 NN denotes cell
T3620 1823-1828 NN denotes death
T3621 1829-1832 IN denotes via
T3622 1833-1837 CC denotes both
T3623 1838-1846 NN denotes necrosis
T3624 1847-1850 CC denotes and
T3625 1851-1860 NN denotes apoptosis
T3626 1861-1862 -LRB- denotes [
T3627 1862-1864 CD denotes 16
T3628 1864-1865 SYM denotes -
T3629 1865-1867 CD denotes 22
T3630 1867-1868 -RRB- denotes ]
T3631 1868-1869 . denotes .
T3632 1869-2021 sentence denotes To date, the literature suggests a complex interplay between caspases and calpains [23,24] and impact of calpain on cell death pathway components [25].
T3633 1870-1872 IN denotes To
T3634 1894-1902 VBZ denotes suggests
T3635 1873-1877 NN denotes date
T3636 1877-1879 , denotes ,
T3637 1879-1882 DT denotes the
T3638 1883-1893 NN denotes literature
T3639 1903-1904 DT denotes a
T3640 1913-1922 NN denotes interplay
T3641 1905-1912 JJ denotes complex
T3642 1923-1930 IN denotes between
T3643 1931-1939 NNS denotes caspases
T3644 1940-1943 CC denotes and
T3645 1944-1952 NNS denotes calpains
T3646 1953-1954 -LRB- denotes [
T3647 1957-1959 CD denotes 24
T3648 1954-1956 CD denotes 23
T3649 1956-1957 , denotes ,
T3650 1959-1960 -RRB- denotes ]
T3651 1961-1964 CC denotes and
T3652 1965-1971 NN denotes impact
T3653 1972-1974 IN denotes of
T3654 1975-1982 NN denotes calpain
T3655 1983-1985 IN denotes on
T3656 1986-1990 NN denotes cell
T3657 1991-1996 NN denotes death
T3658 2005-2015 NNS denotes components
T3659 1997-2004 NN denotes pathway
T3660 2016-2017 -LRB- denotes [
T3661 2017-2019 CD denotes 25
T3662 2019-2020 -RRB- denotes ]
T3663 2020-2021 . denotes .
T3664 2021-2184 sentence denotes The lack of highly specific cell-permeable inhibitors of calpains contributes to the challenge of investigating and defining calpain functions in these processes.
T3665 2022-2025 DT denotes The
T3666 2026-2030 NN denotes lack
T3667 2088-2099 VBZ denotes contributes
T3668 2031-2033 IN denotes of
T3669 2034-2040 RB denotes highly
T3670 2041-2049 JJ denotes specific
T3671 2065-2075 NNS denotes inhibitors
T3672 2050-2054 NN denotes cell
T3673 2055-2064 JJ denotes permeable
T3674 2054-2055 HYPH denotes -
T3675 2076-2078 IN denotes of
T3676 2079-2087 NNS denotes calpains
T3677 2100-2102 IN denotes to
T3678 2103-2106 DT denotes the
T3679 2107-2116 NN denotes challenge
T3680 2117-2119 IN denotes of
T3681 2120-2133 VBG denotes investigating
T3682 2134-2137 CC denotes and
T3683 2138-2146 VBG denotes defining
T3684 2147-2154 NN denotes calpain
T3685 2155-2164 NNS denotes functions
T3686 2165-2167 IN denotes in
T3687 2168-2173 DT denotes these
T3688 2174-2183 NNS denotes processes
T3689 2183-2184 . denotes .
T3690 2184-2391 sentence denotes Although over-expression of calpastatin, the endogenous protein inhibitor of μ- and m-calpain provides an important approach for these efforts, it will not distinguish isoform specific functions [24,26,27].
T3691 2185-2193 IN denotes Although
T3692 2279-2287 VBZ denotes provides
T3693 2194-2209 NN denotes over-expression
T3694 2210-2212 IN denotes of
T3695 2213-2224 NN denotes calpastatin
T3696 2224-2226 , denotes ,
T3697 2226-2229 DT denotes the
T3698 2249-2258 NN denotes inhibitor
T3699 2230-2240 JJ denotes endogenous
T3700 2241-2248 NN denotes protein
T3701 2259-2261 IN denotes of
T3702 2262-2263 NN denotes μ
T3703 2271-2278 NN denotes calpain
T3704 2263-2264 HYPH denotes -
T3705 2265-2268 CC denotes and
T3706 2269-2270 NN denotes m
T3707 2270-2271 HYPH denotes -
T3708 2341-2352 VB denotes distinguish
T3709 2288-2290 DT denotes an
T3710 2301-2309 NN denotes approach
T3711 2291-2300 JJ denotes important
T3712 2310-2313 IN denotes for
T3713 2314-2319 DT denotes these
T3714 2320-2327 NNS denotes efforts
T3715 2327-2329 , denotes ,
T3716 2329-2331 PRP denotes it
T3717 2332-2336 MD denotes will
T3718 2337-2340 RB denotes not
T3719 2353-2360 NN denotes isoform
T3720 2361-2369 JJ denotes specific
T3721 2370-2379 NNS denotes functions
T3722 2380-2381 -LRB- denotes [
T3723 2387-2389 CD denotes 27
T3724 2381-2383 CD denotes 24
T3725 2383-2384 , denotes ,
T3726 2384-2386 CD denotes 26
T3727 2386-2387 , denotes ,
T3728 2389-2390 -RRB- denotes ]
T3729 2390-2391 . denotes .
T3730 2391-2631 sentence denotes Some work has suggested isoform specific roles, such as a role for m-calpain in epidermal growth factor (EGF)-induced cell motility [28,29] and a role for μ-calpain in interferon-inducible protein 9-induced migration of keratinocytes [28].
T3731 2392-2396 DT denotes Some
T3732 2397-2401 NN denotes work
T3733 2406-2415 VBN denotes suggested
T3734 2402-2405 VBZ denotes has
T3735 2416-2423 NN denotes isoform
T3736 2424-2432 JJ denotes specific
T3737 2433-2438 NNS denotes roles
T3738 2438-2440 , denotes ,
T3739 2440-2444 JJ denotes such
T3740 2445-2447 IN denotes as
T3741 2448-2449 DT denotes a
T3742 2450-2454 NN denotes role
T3743 2455-2458 IN denotes for
T3744 2459-2460 NN denotes m
T3745 2461-2468 NN denotes calpain
T3746 2460-2461 HYPH denotes -
T3747 2469-2471 IN denotes in
T3748 2472-2481 JJ denotes epidermal
T3749 2489-2495 NN denotes factor
T3750 2482-2488 NN denotes growth
T3751 2502-2509 VBN denotes induced
T3752 2496-2497 -LRB- denotes (
T3753 2497-2500 NN denotes EGF
T3754 2500-2501 -RRB- denotes )
T3755 2501-2502 HYPH denotes -
T3756 2515-2523 NN denotes motility
T3757 2510-2514 NN denotes cell
T3758 2524-2525 -LRB- denotes [
T3759 2528-2530 CD denotes 29
T3760 2525-2527 CD denotes 28
T3761 2527-2528 , denotes ,
T3762 2530-2531 -RRB- denotes ]
T3763 2532-2535 CC denotes and
T3764 2536-2537 DT denotes a
T3765 2538-2542 NN denotes role
T3766 2543-2546 IN denotes for
T3767 2547-2548 NN denotes μ
T3768 2549-2556 NN denotes calpain
T3769 2548-2549 HYPH denotes -
T3770 2557-2559 IN denotes in
T3771 2560-2570 NN denotes interferon
T3772 2571-2580 JJ denotes inducible
T3773 2570-2571 HYPH denotes -
T3774 2599-2608 NN denotes migration
T3775 2581-2588 NN denotes protein
T3776 2591-2598 VBN denotes induced
T3777 2589-2590 CD denotes 9
T3778 2590-2591 HYPH denotes -
T3779 2609-2611 IN denotes of
T3780 2612-2625 NNS denotes keratinocytes
T3781 2626-2627 -LRB- denotes [
T3782 2627-2629 CD denotes 28
T3783 2629-2630 -RRB- denotes ]
T3784 2630-2631 . denotes .
T3785 2631-2816 sentence denotes A cell permeable calpain inhibitor (which likely inhibits other thiol-proteases as well) has been used to select cells lacking μ-calpain which display reduced proliferation rates [30].
T3786 2632-2633 DT denotes A
T3787 2657-2666 NN denotes inhibitor
T3788 2634-2638 NN denotes cell
T3789 2639-2648 JJ denotes permeable
T3790 2649-2656 NN denotes calpain
T3791 2730-2734 VBN denotes used
T3792 2667-2668 -LRB- denotes (
T3793 2668-2673 WDT denotes which
T3794 2681-2689 VBZ denotes inhibits
T3795 2674-2680 RB denotes likely
T3796 2690-2695 JJ denotes other
T3797 2702-2711 NNS denotes proteases
T3798 2696-2701 NN denotes thiol
T3799 2701-2702 HYPH denotes -
T3800 2712-2714 RB denotes as
T3801 2715-2719 RB denotes well
T3802 2719-2720 -RRB- denotes )
T3803 2721-2724 VBZ denotes has
T3804 2725-2729 VBN denotes been
T3805 2735-2737 TO denotes to
T3806 2738-2744 VB denotes select
T3807 2745-2750 NNS denotes cells
T3808 2751-2758 VBG denotes lacking
T3809 2759-2760 NN denotes μ
T3810 2761-2768 NN denotes calpain
T3811 2760-2761 HYPH denotes -
T3812 2769-2774 WDT denotes which
T3813 2775-2782 VBP denotes display
T3814 2783-2790 VBN denotes reduced
T3815 2805-2810 NNS denotes rates
T3816 2791-2804 NN denotes proliferation
T3817 2811-2812 -LRB- denotes [
T3818 2812-2814 CD denotes 30
T3819 2814-2815 -RRB- denotes ]
T3820 2815-2816 . denotes .
T3821 2816-2949 sentence denotes Interestingly, m-calpain expression persisted in these cells, suggesting a possible requirement of m-calpain for cell survival [30].
T3822 2817-2830 RB denotes Interestingly
T3823 2853-2862 VBD denotes persisted
T3824 2830-2832 , denotes ,
T3825 2832-2833 NN denotes m
T3826 2834-2841 NN denotes calpain
T3827 2833-2834 HYPH denotes -
T3828 2842-2852 NN denotes expression
T3829 2863-2865 IN denotes in
T3830 2866-2871 DT denotes these
T3831 2872-2877 NNS denotes cells
T3832 2877-2879 , denotes ,
T3833 2879-2889 VBG denotes suggesting
T3834 2890-2891 DT denotes a
T3835 2901-2912 NN denotes requirement
T3836 2892-2900 JJ denotes possible
T3837 2913-2915 IN denotes of
T3838 2916-2917 NN denotes m
T3839 2918-2925 NN denotes calpain
T3840 2917-2918 HYPH denotes -
T3841 2926-2929 IN denotes for
T3842 2930-2934 NN denotes cell
T3843 2935-2943 NN denotes survival
T3844 2944-2945 -LRB- denotes [
T3845 2945-2947 CD denotes 30
T3846 2947-2948 -RRB- denotes ]
T3847 2948-2949 . denotes .
T3848 2949-3134 sentence denotes Targeted gene deletion in mice provides a powerful approach to determining the physiological roles of μ- and m-calpain and the opportunity to approach their isoform specific functions.
T3849 2950-2958 VBN denotes Targeted
T3850 2964-2972 NN denotes deletion
T3851 2959-2963 NN denotes gene
T3852 2981-2989 VBZ denotes provides
T3853 2973-2975 IN denotes in
T3854 2976-2980 NNS denotes mice
T3855 2990-2991 DT denotes a
T3856 3001-3009 NN denotes approach
T3857 2992-3000 JJ denotes powerful
T3858 3010-3012 IN denotes to
T3859 3013-3024 VBG denotes determining
T3860 3025-3028 DT denotes the
T3861 3043-3048 NNS denotes roles
T3862 3029-3042 JJ denotes physiological
T3863 3049-3051 IN denotes of
T3864 3052-3053 NN denotes μ
T3865 3061-3068 NN denotes calpain
T3866 3053-3054 HYPH denotes -
T3867 3055-3058 CC denotes and
T3868 3059-3060 NN denotes m
T3869 3060-3061 HYPH denotes -
T3870 3069-3072 CC denotes and
T3871 3073-3076 DT denotes the
T3872 3077-3088 NN denotes opportunity
T3873 3089-3091 TO denotes to
T3874 3092-3100 VB denotes approach
T3875 3101-3106 PRP$ denotes their
T3876 3124-3133 NNS denotes functions
T3877 3107-3114 NN denotes isoform
T3878 3115-3123 JJ denotes specific
T3879 3133-3134 . denotes .
T3880 3134-3272 sentence denotes Initial studies targeted Capn4 based on the prediction that loss of this calpain subunit would abolish activity of both μ- and m-calpain.
T3881 3135-3142 JJ denotes Initial
T3882 3143-3150 NNS denotes studies
T3883 3151-3159 VBD denotes targeted
T3884 3160-3165 NN denotes Capn4
T3885 3166-3171 VBN denotes based
T3886 3172-3174 IN denotes on
T3887 3175-3178 DT denotes the
T3888 3179-3189 NN denotes prediction
T3889 3190-3194 IN denotes that
T3890 3230-3237 VB denotes abolish
T3891 3195-3199 NN denotes loss
T3892 3200-3202 IN denotes of
T3893 3203-3207 DT denotes this
T3894 3216-3223 NN denotes subunit
T3895 3208-3215 NN denotes calpain
T3896 3224-3229 MD denotes would
T3897 3238-3246 NN denotes activity
T3898 3247-3249 IN denotes of
T3899 3250-3254 CC denotes both
T3900 3255-3256 NN denotes μ
T3901 3264-3271 NN denotes calpain
T3902 3256-3257 HYPH denotes -
T3903 3258-3261 CC denotes and
T3904 3262-3263 NN denotes m
T3905 3263-3264 HYPH denotes -
T3906 3271-3272 . denotes .
T3907 3272-3425 sentence denotes Capn4-/- murine embryos died between days 10 and 11 of gestation, and there was no detectable μ- or m-calpain activity in these or younger embryos [31].
T3908 3273-3278 NN denotes Capn4
T3909 3289-3296 NNS denotes embryos
T3910 3278-3279 SYM denotes -
T3911 3279-3280 HYPH denotes /
T3912 3280-3281 SYM denotes -
T3913 3282-3288 JJ denotes murine
T3914 3297-3301 VBD denotes died
T3915 3302-3309 IN denotes between
T3916 3310-3314 NNS denotes days
T3917 3315-3317 CD denotes 10
T3918 3318-3321 CC denotes and
T3919 3322-3324 CD denotes 11
T3920 3325-3327 IN denotes of
T3921 3328-3337 NN denotes gestation
T3922 3337-3339 , denotes ,
T3923 3339-3342 CC denotes and
T3924 3343-3348 EX denotes there
T3925 3349-3352 VBD denotes was
T3926 3353-3355 DT denotes no
T3927 3383-3391 NN denotes activity
T3928 3356-3366 JJ denotes detectable
T3929 3367-3368 NN denotes μ
T3930 3375-3382 NN denotes calpain
T3931 3368-3369 HYPH denotes -
T3932 3370-3372 CC denotes or
T3933 3373-3374 NN denotes m
T3934 3374-3375 HYPH denotes -
T3935 3392-3394 IN denotes in
T3936 3395-3400 DT denotes these
T3937 3401-3403 CC denotes or
T3938 3404-3411 JJR denotes younger
T3939 3412-3419 NNS denotes embryos
T3940 3420-3421 -LRB- denotes [
T3941 3421-3423 CD denotes 31
T3942 3423-3424 -RRB- denotes ]
T3943 3424-3425 . denotes .
T3944 3425-3758 sentence denotes Capn4-/- murine embryonic fibroblasts (MEFs) could be cultured from these embryos, although they also lacked calpain activity as assessed by casein zymography or by the formation of characteristic spectrin breakdown products, and they displayed migration defects consistent with a role for calpain in release of focal adhesions [9].
T3945 3426-3431 NN denotes Capn4
T3946 3480-3488 VBN denotes cultured
T3947 3431-3432 SYM denotes -
T3948 3432-3433 HYPH denotes /
T3949 3433-3434 SYM denotes -
T3950 3435-3441 JJ denotes murine
T3951 3452-3463 NNS denotes fibroblasts
T3952 3442-3451 JJ denotes embryonic
T3953 3464-3465 -LRB- denotes (
T3954 3465-3469 NNS denotes MEFs
T3955 3469-3470 -RRB- denotes )
T3956 3471-3476 MD denotes could
T3957 3477-3479 VB denotes be
T3958 3489-3493 IN denotes from
T3959 3494-3499 DT denotes these
T3960 3500-3507 NNS denotes embryos
T3961 3507-3509 , denotes ,
T3962 3509-3517 IN denotes although
T3963 3528-3534 VBD denotes lacked
T3964 3518-3522 PRP denotes they
T3965 3523-3527 RB denotes also
T3966 3535-3542 NN denotes calpain
T3967 3543-3551 NN denotes activity
T3968 3552-3554 IN denotes as
T3969 3555-3563 VBN denotes assessed
T3970 3564-3566 IN denotes by
T3971 3567-3573 NN denotes casein
T3972 3574-3584 NN denotes zymography
T3973 3585-3587 CC denotes or
T3974 3588-3590 IN denotes by
T3975 3591-3594 DT denotes the
T3976 3595-3604 NN denotes formation
T3977 3605-3607 IN denotes of
T3978 3608-3622 JJ denotes characteristic
T3979 3642-3650 NNS denotes products
T3980 3623-3631 NN denotes spectrin
T3981 3632-3641 NN denotes breakdown
T3982 3650-3652 , denotes ,
T3983 3652-3655 CC denotes and
T3984 3656-3660 PRP denotes they
T3985 3661-3670 VBD denotes displayed
T3986 3671-3680 NN denotes migration
T3987 3681-3688 NNS denotes defects
T3988 3689-3699 JJ denotes consistent
T3989 3700-3704 IN denotes with
T3990 3705-3706 DT denotes a
T3991 3707-3711 NN denotes role
T3992 3712-3715 IN denotes for
T3993 3716-3723 NN denotes calpain
T3994 3724-3726 IN denotes in
T3995 3727-3734 NN denotes release
T3996 3735-3737 IN denotes of
T3997 3738-3743 JJ denotes focal
T3998 3744-3753 NNS denotes adhesions
T3999 3754-3755 -LRB- denotes [
T4000 3755-3756 CD denotes 9
T4001 3756-3757 -RRB- denotes ]
T4002 3757-3758 . denotes .
T4003 3758-3937 sentence denotes An independently derived Capn4 knockout, involving a more extensive deletion of the gene, resulted in an earlier embryonic lethality, apparently at a pre-implantation stage [32].
T4004 3759-3761 DT denotes An
T4005 3790-3798 NN denotes knockout
T4006 3762-3775 RB denotes independently
T4007 3776-3783 VBN denotes derived
T4008 3784-3789 NN denotes Capn4
T4009 3849-3857 VBD denotes resulted
T4010 3798-3800 , denotes ,
T4011 3800-3809 VBG denotes involving
T4012 3810-3811 DT denotes a
T4013 3827-3835 NN denotes deletion
T4014 3812-3816 RBR denotes more
T4015 3817-3826 JJ denotes extensive
T4016 3836-3838 IN denotes of
T4017 3839-3842 DT denotes the
T4018 3843-3847 NN denotes gene
T4019 3847-3849 , denotes ,
T4020 3858-3860 IN denotes in
T4021 3861-3863 DT denotes an
T4022 3882-3891 NN denotes lethality
T4023 3864-3871 JJR denotes earlier
T4024 3872-3881 JJ denotes embryonic
T4025 3891-3893 , denotes ,
T4026 3893-3903 RB denotes apparently
T4027 3904-3906 IN denotes at
T4028 3907-3908 DT denotes a
T4029 3926-3931 NN denotes stage
T4030 3909-3925 JJ denotes pre-implantation
T4031 3932-3933 -LRB- denotes [
T4032 3933-3935 CD denotes 32
T4033 3935-3936 -RRB- denotes ]
T4034 3936-3937 . denotes .
T4035 3937-4254 sentence denotes The different times of embryonic lethality suggested that the first reported Capn4-/- mice [31] were targeted with a hypomorphic mutation, which retained some small level of calpain activity, allowing for their survival to mid-gestation, while the second reported Capn4-/- mice [32] represented a true null mutation.
T4036 3938-3941 DT denotes The
T4037 3952-3957 NNS denotes times
T4038 3942-3951 JJ denotes different
T4039 3981-3990 VBD denotes suggested
T4040 3958-3960 IN denotes of
T4041 3961-3970 JJ denotes embryonic
T4042 3971-3980 NN denotes lethality
T4043 3991-3995 IN denotes that
T4044 4039-4047 VBN denotes targeted
T4045 3996-3999 DT denotes the
T4046 4024-4028 NNS denotes mice
T4047 4000-4005 JJ denotes first
T4048 4006-4014 VBN denotes reported
T4049 4015-4020 NN denotes Capn4
T4050 4020-4021 SYM denotes -
T4051 4021-4022 HYPH denotes /
T4052 4022-4023 SYM denotes -
T4053 4029-4030 -LRB- denotes [
T4054 4030-4032 CD denotes 31
T4055 4032-4033 -RRB- denotes ]
T4056 4034-4038 VBD denotes were
T4057 4048-4052 IN denotes with
T4058 4053-4054 DT denotes a
T4059 4067-4075 NN denotes mutation
T4060 4055-4066 JJ denotes hypomorphic
T4061 4075-4077 , denotes ,
T4062 4077-4082 WDT denotes which
T4063 4083-4091 VBD denotes retained
T4064 4092-4096 DT denotes some
T4065 4103-4108 NN denotes level
T4066 4097-4102 JJ denotes small
T4067 4109-4111 IN denotes of
T4068 4112-4119 NN denotes calpain
T4069 4120-4128 NN denotes activity
T4070 4128-4130 , denotes ,
T4071 4130-4138 VBG denotes allowing
T4072 4139-4142 IN denotes for
T4073 4143-4148 PRP$ denotes their
T4074 4149-4157 NN denotes survival
T4075 4158-4160 IN denotes to
T4076 4161-4174 NN denotes mid-gestation
T4077 4174-4176 , denotes ,
T4078 4176-4181 IN denotes while
T4079 4221-4232 VBD denotes represented
T4080 4182-4185 DT denotes the
T4081 4211-4215 NNS denotes mice
T4082 4186-4192 JJ denotes second
T4083 4193-4201 VBN denotes reported
T4084 4202-4207 NN denotes Capn4
T4085 4207-4208 SYM denotes -
T4086 4208-4209 HYPH denotes /
T4087 4209-4210 SYM denotes -
T4088 4216-4217 -LRB- denotes [
T4089 4217-4219 CD denotes 32
T4090 4219-4220 -RRB- denotes ]
T4091 4233-4234 DT denotes a
T4092 4245-4253 NN denotes mutation
T4093 4235-4239 JJ denotes true
T4094 4240-4244 JJ denotes null
T4095 4253-4254 . denotes .
T4096 4254-4488 sentence denotes Disruption of Capn1, encoding the μ-calpain catalytic subunit, was subsequently reported to result in fertile, viable mice with some mild defects in the μ-calpain rich platelets relating to their aggregation and clot retraction [33].
T4097 4255-4265 NN denotes Disruption
T4098 4335-4343 VBN denotes reported
T4099 4266-4268 IN denotes of
T4100 4269-4274 NN denotes Capn1
T4101 4274-4276 , denotes ,
T4102 4276-4284 VBG denotes encoding
T4103 4285-4288 DT denotes the
T4104 4309-4316 NN denotes subunit
T4105 4289-4290 NN denotes μ
T4106 4291-4298 NN denotes calpain
T4107 4290-4291 HYPH denotes -
T4108 4299-4308 JJ denotes catalytic
T4109 4316-4318 , denotes ,
T4110 4318-4321 VBD denotes was
T4111 4322-4334 RB denotes subsequently
T4112 4344-4346 TO denotes to
T4113 4347-4353 VB denotes result
T4114 4354-4356 IN denotes in
T4115 4357-4364 JJ denotes fertile
T4116 4373-4377 NNS denotes mice
T4117 4364-4366 , denotes ,
T4118 4366-4372 JJ denotes viable
T4119 4378-4382 IN denotes with
T4120 4383-4387 DT denotes some
T4121 4393-4400 NNS denotes defects
T4122 4388-4392 JJ denotes mild
T4123 4401-4403 IN denotes in
T4124 4404-4407 DT denotes the
T4125 4423-4432 NNS denotes platelets
T4126 4408-4409 NN denotes μ
T4127 4410-4417 NN denotes calpain
T4128 4409-4410 HYPH denotes -
T4129 4418-4422 JJ denotes rich
T4130 4433-4441 VBG denotes relating
T4131 4442-4444 IN denotes to
T4132 4445-4450 PRP$ denotes their
T4133 4451-4462 NN denotes aggregation
T4134 4463-4466 CC denotes and
T4135 4467-4471 NN denotes clot
T4136 4472-4482 NN denotes retraction
T4137 4483-4484 -LRB- denotes [
T4138 4484-4486 CD denotes 33
T4139 4486-4487 -RRB- denotes ]
T4140 4487-4488 . denotes .
T4141 4488-4636 sentence denotes The fact that Capn4 null mice die during embryogenesis indicates that at least one of the ubiquitous calpains is essential for development to term.
T4142 4489-4492 DT denotes The
T4143 4493-4497 NN denotes fact
T4144 4544-4553 VBZ denotes indicates
T4145 4498-4502 IN denotes that
T4146 4519-4522 VBP denotes die
T4147 4503-4508 NN denotes Capn4
T4148 4509-4513 JJ denotes null
T4149 4514-4518 NNS denotes mice
T4150 4523-4529 IN denotes during
T4151 4530-4543 NN denotes embryogenesis
T4152 4554-4558 IN denotes that
T4153 4599-4601 VBZ denotes is
T4154 4559-4561 RB denotes at
T4155 4568-4571 CD denotes one
T4156 4562-4567 RBS denotes least
T4157 4572-4574 IN denotes of
T4158 4575-4578 DT denotes the
T4159 4590-4598 NNS denotes calpains
T4160 4579-4589 JJ denotes ubiquitous
T4161 4602-4611 JJ denotes essential
T4162 4612-4615 IN denotes for
T4163 4616-4627 NN denotes development
T4164 4628-4630 IN denotes to
T4165 4631-4635 NN denotes term
T4166 4635-4636 . denotes .
T4167 4636-4899 sentence denotes The viability of Capn1-deficient mice does not however distinguish between two possibilities: either that m-calpain is specifically required during embryogenesis, or that either form of calpain alone is sufficient and can compensate for the absence of the other.
T4168 4637-4640 DT denotes The
T4169 4641-4650 NN denotes viability
T4170 4692-4703 VB denotes distinguish
T4171 4651-4653 IN denotes of
T4172 4654-4659 NN denotes Capn1
T4173 4660-4669 JJ denotes deficient
T4174 4659-4660 HYPH denotes -
T4175 4670-4674 NNS denotes mice
T4176 4675-4679 VBZ denotes does
T4177 4680-4683 RB denotes not
T4178 4684-4691 RB denotes however
T4179 4704-4711 IN denotes between
T4180 4712-4715 CD denotes two
T4181 4716-4729 NNS denotes possibilities
T4182 4729-4731 : denotes :
T4183 4731-4737 CC denotes either
T4184 4769-4777 VBN denotes required
T4185 4738-4742 IN denotes that
T4186 4743-4744 NN denotes m
T4187 4745-4752 NN denotes calpain
T4188 4744-4745 HYPH denotes -
T4189 4753-4755 VBZ denotes is
T4190 4756-4768 RB denotes specifically
T4191 4778-4784 IN denotes during
T4192 4785-4798 NN denotes embryogenesis
T4193 4798-4800 , denotes ,
T4194 4800-4802 CC denotes or
T4195 4803-4807 IN denotes that
T4196 4837-4839 VBZ denotes is
T4197 4808-4814 CC denotes either
T4198 4815-4819 NN denotes form
T4199 4820-4822 IN denotes of
T4200 4823-4830 NN denotes calpain
T4201 4831-4836 RB denotes alone
T4202 4840-4850 JJ denotes sufficient
T4203 4851-4854 CC denotes and
T4204 4855-4858 MD denotes can
T4205 4859-4869 VB denotes compensate
T4206 4870-4873 IN denotes for
T4207 4874-4877 DT denotes the
T4208 4878-4885 NN denotes absence
T4209 4886-4888 IN denotes of
T4210 4889-4892 DT denotes the
T4211 4893-4898 JJ denotes other
T4212 4898-4899 . denotes .
T4213 4899-5001 sentence denotes To resolve this question, we have now knocked out the Capn2 gene encoding the m-80 k subunit in mice.
T4214 4900-4902 TO denotes To
T4215 4903-4910 VB denotes resolve
T4216 4938-4945 VBN denotes knocked
T4217 4911-4915 DT denotes this
T4218 4916-4924 NN denotes question
T4219 4924-4926 , denotes ,
T4220 4926-4928 PRP denotes we
T4221 4929-4933 VBP denotes have
T4222 4934-4937 RB denotes now
T4223 4946-4949 RP denotes out
T4224 4950-4953 DT denotes the
T4225 4960-4964 NN denotes gene
T4226 4954-4959 NN denotes Capn2
T4227 4965-4973 VBG denotes encoding
T4228 4974-4977 DT denotes the
T4229 4985-4992 NN denotes subunit
T4230 4978-4979 NN denotes m
T4231 4983-4984 NN denotes k
T4232 4979-4980 HYPH denotes -
T4233 4980-4982 CD denotes 80
T4234 4993-4995 IN denotes in
T4235 4996-5000 NNS denotes mice
T4236 5000-5001 . denotes .
T4237 5001-5146 sentence denotes We report here that Capn2 null embryos died prior to the implantation stage, indicating that m-calpain is indispensable for early embryogenesis.
T4238 5002-5004 PRP denotes We
T4239 5005-5011 VBP denotes report
T4240 5012-5016 RB denotes here
T4241 5017-5021 IN denotes that
T4242 5041-5045 VBD denotes died
T4243 5022-5027 NN denotes Capn2
T4244 5028-5032 JJ denotes null
T4245 5033-5040 NNS denotes embryos
T4246 5046-5051 JJ denotes prior
T4247 5052-5054 IN denotes to
T4248 5055-5058 DT denotes the
T4249 5072-5077 NN denotes stage
T4250 5059-5071 NN denotes implantation
T4251 5077-5079 , denotes ,
T4252 5079-5089 VBG denotes indicating
T4253 5090-5094 IN denotes that
T4254 5105-5107 VBZ denotes is
T4255 5095-5096 NN denotes m
T4256 5097-5104 NN denotes calpain
T4257 5096-5097 HYPH denotes -
T4258 5108-5121 JJ denotes indispensable
T4259 5122-5125 IN denotes for
T4260 5126-5131 JJ denotes early
T4261 5132-5145 NN denotes embryogenesis
T4262 5145-5146 . denotes .
T4263 5146-5300 sentence denotes This role cannot be fulfilled by μ-calpain, which is expressed in embryonic stem (ES) cells [31] and is assumed to be present at this stage of gestation.
T4264 5147-5151 DT denotes This
T4265 5152-5156 NN denotes role
T4266 5167-5176 VBN denotes fulfilled
T4267 5157-5160 MD denotes can
T4268 5160-5163 RB denotes not
T4269 5164-5166 VB denotes be
T4270 5177-5179 IN denotes by
T4271 5180-5181 NN denotes μ
T4272 5182-5189 NN denotes calpain
T4273 5181-5182 HYPH denotes -
T4274 5189-5191 , denotes ,
T4275 5191-5196 WDT denotes which
T4276 5200-5209 VBN denotes expressed
T4277 5197-5199 VBZ denotes is
T4278 5210-5212 IN denotes in
T4279 5213-5222 JJ denotes embryonic
T4280 5223-5227 NN denotes stem
T4281 5233-5238 NNS denotes cells
T4282 5228-5229 -LRB- denotes (
T4283 5229-5231 NN denotes ES
T4284 5231-5232 -RRB- denotes )
T4285 5239-5240 -LRB- denotes [
T4286 5240-5242 CD denotes 31
T4287 5242-5243 -RRB- denotes ]
T4288 5244-5247 CC denotes and
T4289 5248-5250 VBZ denotes is
T4290 5251-5258 VBN denotes assumed
T4291 5259-5261 TO denotes to
T4292 5262-5264 VB denotes be
T4293 5265-5272 JJ denotes present
T4294 5273-5275 IN denotes at
T4295 5276-5280 DT denotes this
T4296 5281-5286 NN denotes stage
T4297 5287-5289 IN denotes of
T4298 5290-5299 NN denotes gestation
T4299 5299-5300 . denotes .
T4300 5300-5423 sentence denotes This demonstrates unequivocally that m-calpain and μ-calpain have distinct physiological roles during early embryogenesis.
T4301 5301-5305 DT denotes This
T4302 5306-5318 VBZ denotes demonstrates
T4303 5319-5332 RB denotes unequivocally
T4304 5333-5337 IN denotes that
T4305 5362-5366 VBP denotes have
T4306 5338-5339 NN denotes m
T4307 5340-5347 NN denotes calpain
T4308 5339-5340 HYPH denotes -
T4309 5348-5351 CC denotes and
T4310 5352-5353 NN denotes μ
T4311 5354-5361 NN denotes calpain
T4312 5353-5354 HYPH denotes -
T4313 5367-5375 JJ denotes distinct
T4314 5390-5395 NNS denotes roles
T4315 5376-5389 JJ denotes physiological
T4316 5396-5402 IN denotes during
T4317 5403-5408 JJ denotes early
T4318 5409-5422 NN denotes embryogenesis
T4319 5422-5423 . denotes .
R282 T3323 T3320 nummod 80,k
R287 T3324 T3320 punct ),k
R288 T3325 T3306 punct ", ",encoded
R289 T3326 T3306 advmod respectively,encoded
R290 T3244 T3245 det The,proteases
R291 T3327 T3301 punct ", ",subunits
R292 T3245 T3254 nsubj proteases,are
R293 T3246 T3245 nummod two,proteases
R294 T3328 T3329 dep that,associate
R295 T3247 T3245 amod ubiquitous,proteases
R296 T3248 T3249 npadvmod Ca2,dependent
R297 T3329 T3301 relcl associate,subunits
R298 T3249 T3245 amod dependent,proteases
R299 T3250 T3248 punct +,Ca2
R300 T3251 T3249 punct -,dependent
R301 T3330 T3329 prep with,associate
R302 T3252 T3245 punct ", ",proteases
R303 T3253 T3245 compound cysteine,proteases
R304 T3255 T3245 acl known,proteases
R305 T3331 T3332 det a,subunit
R306 T3256 T3255 prep as,known
R307 T3257 T3258 compound μ,calpain
R308 T3258 T3256 pobj calpain,as
R309 T3332 T3330 pobj subunit,with
R310 T3259 T3258 punct -,calpain
R311 T3260 T3258 punct (,calpain
R312 T3261 T3258 appos calpain,calpain
R313 T3333 T3332 amod common,subunit
R314 T3262 T3261 punct -,calpain
R315 T3263 T3261 nummod 1,calpain
R316 T3264 T3258 punct ),calpain
R317 T3334 T3335 nummod 28,kDa
R318 T3265 T3258 cc and,calpain
R319 T3335 T3332 nmod kDa,subunit
R320 T3266 T3267 compound m,calpain
R321 T3267 T3258 conj calpain,calpain
R322 T3268 T3267 punct -,calpain
R323 T3269 T3267 punct (,calpain
R324 T3270 T3267 appos capain,calpain
R325 T3271 T3270 punct -,capain
R326 T3272 T3270 nummod 2,capain
R327 T3273 T3254 punct ),are
R328 T3274 T3254 punct ", ",are
R329 T3275 T3276 det the,members
R330 T3276 T3254 attr members,are
R331 T3277 T3276 compound founding,members
R332 T3278 T3276 prep of,members
R333 T3336 T3332 amod regulatory,subunit
R334 T3279 T3280 det a,family
R335 T3280 T3278 pobj family,of
R336 T3281 T3280 compound gene,family
R337 T3282 T3280 acl comprising,family
R338 T3337 T3332 acl encoded,subunit
R339 T3283 T3284 nummod 13,genes
R340 T3284 T3282 dobj genes,comprising
R341 T3285 T3282 prep in,comprising
R342 T3338 T3337 agent by,encoded
R343 T3286 T3285 pobj mammals,in
R344 T3287 T3288 punct [,1
R345 T3288 T3254 parataxis 1,are
R346 T3339 T3340 det the,gene
R347 T3289 T3290 punct -,3
R348 T3290 T3288 prep 3,1
R349 T3291 T3288 punct ],1
R350 T3340 T3338 pobj gene,by
R351 T3292 T3254 punct .,are
R352 T3341 T3340 compound Capn4,gene
R353 T3294 T3295 nsubj Both,are
R354 T3296 T3297 amod heterodimeric,enzymes
R355 T3342 T3295 punct .,are
R356 T3297 T3295 attr enzymes,are
R357 T3298 T3297 acl consisting,enzymes
R358 T3299 T3298 prep of,consisting
R359 T3344 T3345 det The,subunits
R360 T3300 T3301 amod distinct,subunits
R361 T3301 T3299 pobj subunits,of
R362 T3302 T3303 nummod 80,kDa
R363 T3303 T3301 nmod kDa,subunits
R364 T3345 T3355 nsubj subunits,share
R365 T3304 T3301 amod catalytic,subunits
R366 T3305 T3301 punct ", ",subunits
R367 T3346 T3347 nmod μ,k
R368 T3347 T3345 nmod k,subunits
R369 T3306 T3301 acl encoded,subunits
R370 T3307 T3306 agent by,encoded
R371 T3348 T3346 punct -,μ
R372 T3308 T3309 det the,genes
R373 T3309 T3307 pobj genes,by
R374 T3310 T3309 nmod Capn1,genes
R375 T3311 T3312 punct (,k
R376 T3349 T3346 nummod 80,μ
R377 T3312 T3310 parataxis k,Capn1
R378 T3313 T3312 nmod μ,k
R379 T3314 T3312 punct -,k
R380 T3315 T3312 nummod 80,k
R381 T3350 T3347 cc and,k
R382 T3316 T3312 punct ),k
R383 T3317 T3310 cc and,Capn1
R384 T3318 T3310 conj Capn2,Capn1
R385 T3351 T3352 nmod m,k
R386 T3319 T3320 punct (,k
R387 T3320 T3318 parataxis k,Capn2
R388 T3321 T3320 nmod m,k
R389 T3352 T3347 conj k,k
R390 T3322 T3320 punct -,k
R391 T3353 T3351 punct -,m
R392 T3354 T3351 nummod 80,m
R393 T3356 T3357 nummod 62,%
R394 T3429 T3419 punct ;,levels
R395 T3357 T3358 compound %,identity
R396 T3430 T3431 quantmod 10,50
R397 T3431 T3433 nummod 50,μM
R398 T3358 T3355 dobj identity,share
R399 T3432 T3431 punct –,50
R400 T3433 T3434 compound μM,Ca2
R401 T3434 T3419 appos Ca2,levels
R402 T3359 T3360 compound amino,acid
R403 T3435 T3434 punct +,Ca2
R404 T3436 T3434 prep for,Ca2
R405 T3437 T3438 compound μ,calpain
R406 T3360 T3358 compound acid,identity
R407 T3438 T3436 pobj calpain,for
R408 T3439 T3438 punct -,calpain
R409 T3440 T3434 cc and,Ca2
R410 T3361 T3358 compound sequence,identity
R411 T3441 T3442 quantmod 0.3,0.35
R412 T3442 T3444 nummod 0.35,mM
R413 T3443 T3442 punct –,0.35
R414 T3362 T3355 punct ", ",share
R415 T3444 T3445 compound mM,Ca2
R416 T3445 T3434 conj Ca2,Ca2
R417 T3363 T3355 cc and,share
R418 T3446 T3445 punct +,Ca2
R419 T3447 T3445 prep for,Ca2
R420 T3448 T3449 compound m,calpain
R421 T3449 T3447 pobj calpain,for
R422 T3364 T3355 conj are,share
R423 T3450 T3449 punct -,calpain
R424 T3451 T3416 punct .,derive
R425 T3453 T3454 nsubjpass It,assumed
R426 T3365 T3366 advmod very,similar
R427 T3455 T3454 auxpass is,assumed
R428 T3456 T3454 advmod generally,assumed
R429 T3457 T3458 mark that,maintain
R430 T3366 T3364 acomp similar,are
R431 T3458 T3454 ccomp maintain,assumed
R432 T3459 T3460 nmod μ,calpain
R433 T3367 T3364 prep in,are
R434 T3460 T3458 nsubj calpain,maintain
R435 T3461 T3459 punct -,μ
R436 T3462 T3459 cc and,μ
R437 T3463 T3459 conj m,μ
R438 T3368 T3367 pobj terms,in
R439 T3464 T3460 punct -,calpain
R440 T3465 T3466 poss their,sensitivities
R441 T3466 T3458 dobj sensitivities,maintain
R442 T3369 T3368 prep of,terms
R443 T3467 T3466 amod differential,sensitivities
R444 T3468 T3466 prep to,sensitivities
R445 T3370 T3369 pobj structure,of
R446 T3469 T3468 pobj calcium,to
R447 T3470 T3471 advmod in,vivo
R448 T3471 T3458 advmod vivo,maintain
R449 T3371 T3370 punct ", ",structure
R450 T3472 T3458 punct ", ",maintain
R451 T3473 T3474 mark although,demonstrated
R452 T3372 T3373 compound protein,chemistry
R453 T3373 T3370 conj chemistry,structure
R454 T3474 T3458 advcl demonstrated,maintain
R455 T3475 T3474 nsubjpass this,demonstrated
R456 T3476 T3474 aux has,demonstrated
R457 T3374 T3373 punct ", ",chemistry
R458 T3477 T3474 neg not,demonstrated
R459 T3478 T3474 advmod yet,demonstrated
R460 T3375 T3373 cc and,chemistry
R461 T3479 T3474 auxpass been,demonstrated
R462 T3480 T3474 advmod strictly,demonstrated
R463 T3481 T3454 punct .,assumed
R464 T3376 T3377 advmod in,vitro
R465 T3483 T3484 advmod Furthermore,assumed
R466 T3377 T3378 amod vitro,specificity
R467 T3485 T3484 punct ", ",assumed
R468 T3486 T3487 mark since,is
R469 T3378 T3373 conj specificity,chemistry
R470 T3487 T3484 advcl is,assumed
R471 T3488 T3489 det the,concentration
R472 T3379 T3378 compound substrate,specificity
R473 T3489 T3487 nsubj concentration,is
R474 T3490 T3491 amod cytoplasmic,free
R475 T3491 T3489 amod free,concentration
R476 T3380 T3355 punct .,share
R477 T3492 T3489 nmod Ca2,concentration
R478 T3493 T3492 punct +,Ca2
R479 T3494 T3487 advmod typically,is
R480 T3495 T3496 amod less,1
R481 T3496 T3498 nummod 1,μM
R482 T3497 T3496 quantmod than,1
R483 T3382 T3383 prep Despite,suggest
R484 T3498 T3487 attr μM,is
R485 T3499 T3484 punct ", ",assumed
R486 T3500 T3484 nsubjpass it,assumed
R487 T3501 T3484 auxpass is,assumed
R488 T3384 T3385 det these,similarities
R489 T3502 T3484 advmod also,assumed
R490 T3503 T3504 mark that,contribute
R491 T3504 T3484 ccomp contribute,assumed
R492 T3505 T3506 amod other,factors
R493 T3506 T3504 nsubj factors,contribute
R494 T3385 T3382 pobj similarities,Despite
R495 T3507 T3508 advmod in,vivo
R496 T3508 T3506 amod vivo,factors
R497 T3509 T3504 aux must,contribute
R498 T3510 T3504 prep to,contribute
R499 T3386 T3383 punct ", ",suggest
R500 T3511 T3510 pobj regulation,to
R501 T3512 T3511 prep of,regulation
R502 T3387 T3388 det the,patterns
R503 T3513 T3514 det these,enzymes
R504 T3514 T3512 pobj enzymes,of
R505 T3515 T3516 punct [,3
R506 T3388 T3383 nsubj patterns,suggest
R507 T3516 T3504 parataxis 3,contribute
R508 T3517 T3516 punct ],3
R509 T3518 T3484 punct .,assumed
R510 T3520 T3521 prep Without,presents
R511 T3389 T3388 amod differential,patterns
R512 T3522 T3523 nmod apriori,knowledge
R513 T3523 T3520 pobj knowledge,Without
R514 T3390 T3388 compound expression,patterns
R515 T3524 T3523 prep of,knowledge
R516 T3525 T3526 det the,factors
R517 T3526 T3524 pobj factors,of
R518 T3391 T3388 prep of,patterns
R519 T3527 T3526 acl regulating,factors
R520 T3528 T3529 compound calpain,activity
R521 T3529 T3527 dobj activity,regulating
R522 T3392 T3393 nmod μ,calpain
R523 T3530 T3526 cc or,factors
R524 T3531 T3532 poss their,substrates
R525 T3532 T3526 conj substrates,factors
R526 T3393 T3391 pobj calpain,of
R527 T3533 T3532 amod relevant,substrates
R528 T3534 T3521 punct ", ",presents
R529 T3394 T3392 punct -,μ
R530 T3535 T3521 nsubj elucidation,presents
R531 T3395 T3392 cc and,μ
R532 T3536 T3535 prep of,elucidation
R533 T3537 T3538 amod biological,functions
R534 T3538 T3536 pobj functions,of
R535 T3539 T3535 prep for,elucidation
R536 T3396 T3392 conj m,μ
R537 T3540 T3539 pobj calpains,for
R538 T3541 T3542 det a,challenge
R539 T3542 T3521 dobj challenge,presents
R540 T3397 T3393 punct -,calpain
R541 T3543 T3521 punct .,presents
R542 T3398 T3388 prep in,patterns
R543 T3545 T3546 nsubj Research,linked
R544 T3547 T3545 prep on,Research
R545 T3399 T3400 amod mammalian,tissues
R546 T3548 T3547 pobj calpains,on
R547 T3549 T3546 aux has,linked
R548 T3550 T3546 dobj them,linked
R549 T3400 T3398 pobj tissues,in
R550 T3551 T3546 prep with,linked
R551 T3552 T3553 det a,variety
R552 T3401 T3402 nsubj they,have
R553 T3553 T3551 pobj variety,with
R554 T3554 T3553 amod wide,variety
R555 T3555 T3553 prep of,variety
R556 T3402 T3383 advcl have,suggest
R557 T3556 T3555 pobj functions,of
R558 T3557 T3553 prep including,variety
R559 T3558 T3559 compound muscle,growth
R560 T3403 T3404 det some,functions
R561 T3559 T3557 pobj growth,including
R562 T3404 T3402 dobj functions,have
R563 T3560 T3559 punct ", ",growth
R564 T3405 T3406 npadvmod isoform,specific
R565 T3561 T3559 conj development,growth
R566 T3562 T3561 punct ", ",development
R567 T3563 T3561 conj degeneration,development
R568 T3406 T3404 amod specific,functions
R569 T3564 T3565 punct (,3
R570 T3407 T3406 cc and,specific
R571 T3565 T3563 parataxis 3,degeneration
R572 T3408 T3406 conj distinct,specific
R573 T3566 T3565 punct ),3
R574 T3567 T3563 punct ", ",degeneration
R575 T3409 T3383 punct .,suggest
R578 T3411 T3412 det The,designations
R579 T3570 T3569 cc and,growth
R580 T3571 T3569 conj neurodegeneration,growth
R581 T3572 T3573 punct [,4
R582 T3573 T3571 parataxis 4,neurodegeneration
R583 T3574 T3573 punct ],4
R584 T3575 T3569 punct ", ",growth
R585 T3576 T3577 compound cell,cycle
R586 T3412 T3416 nsubj designations,derive
R587 T3577 T3578 compound cycle,progression
R588 T3578 T3569 conj progression,growth
R589 T3413 T3412 nmod μ,designations
R590 T3579 T3580 punct [,6
R591 T3580 T3578 parataxis 6,progression
R592 T3581 T3580 nummod 5,6
R593 T3414 T3413 cc and,μ
R594 T3582 T3580 punct ",",6
R595 T3583 T3580 punct ],6
R596 T3584 T3578 punct ", ",progression
R597 T3415 T3413 conj m,μ
R598 T3585 T3586 compound signal,cascades
R599 T3586 T3578 conj cascades,progression
R600 T3587 T3586 acl triggered,cascades
R601 T3417 T3416 prep from,derive
R602 T3588 T3587 agent by,triggered
R603 T3589 T3588 pobj integrins,by
R604 T3590 T3589 cc and,integrins
R605 T3591 T3592 compound growth,factors
R606 T3418 T3419 det the,levels
R607 T3592 T3589 conj factors,integrins
R608 T3593 T3594 punct [,7
R609 T3594 T3587 parataxis 7,triggered
R610 T3419 T3417 pobj levels,from
R611 T3595 T3594 punct ],7
R612 T3596 T3586 punct ", ",cascades
R613 T3597 T3598 compound membrane,protrusion
R614 T3598 T3586 conj protrusion,cascades
R615 T3420 T3419 prep of,levels
R616 T3599 T3600 punct [,8
R617 T3600 T3598 parataxis 8,protrusion
R618 T3421 T3420 pobj Ca2,of
R619 T3601 T3600 punct ],8
R620 T3602 T3598 punct ", ",protrusion
R621 T3603 T3598 conj remodeling,protrusion
R622 T3422 T3421 punct +,Ca2
R623 T3604 T3603 prep of,remodeling
R624 T3605 T3606 det the,cytoskeleton
R625 T3606 T3604 pobj cytoskeleton,of
R626 T3423 T3419 acl required,levels
R627 T3607 T3603 cc and,remodeling
R628 T3608 T3609 compound cell,migration
R629 T3609 T3603 conj migration,remodeling
R630 T3424 T3425 advmod in,vitro
R631 T3610 T3611 punct [,9
R632 T3611 T3609 parataxis 9,migration
R633 T3612 T3613 punct -,15
R634 T3613 T3611 appos 15,9
R635 T3614 T3611 punct ],9
R636 T3615 T3603 punct ", ",remodeling
R637 T3425 T3423 advmod vitro,required
R638 T3616 T3603 cc and,remodeling
R639 T3617 T3603 conj regulation,remodeling
R640 T3426 T3423 prep for,required
R641 T3618 T3617 prep of,regulation
R642 T3619 T3620 compound cell,death
R643 T3620 T3618 pobj death,of
R644 T3621 T3617 prep via,regulation
R645 T3427 T3428 amod optimal,activation
R646 T3622 T3623 preconj both,necrosis
R647 T3623 T3621 pobj necrosis,via
R648 T3624 T3623 cc and,necrosis
R649 T3428 T3426 pobj activation,for
R650 T3625 T3623 conj apoptosis,necrosis
R651 T3626 T3627 punct [,16
R652 T3627 T3617 parataxis 16,regulation
R653 T3640 T3634 dobj interplay,suggests
R654 T3628 T3629 punct -,22
R655 T3629 T3627 appos 22,16
R656 T3630 T3627 punct ],16
R657 T3631 T3546 punct .,linked
R658 T3641 T3640 amod complex,interplay
R659 T3633 T3634 prep To,suggests
R660 T3642 T3640 prep between,interplay
R661 T3635 T3633 pobj date,To
R662 T3636 T3634 punct ", ",suggests
R663 T3643 T3642 pobj caspases,between
R664 T3637 T3638 det the,literature
R665 T3638 T3634 nsubj literature,suggests
R666 T3639 T3640 det a,interplay
R667 T3644 T3643 cc and,caspases
R668 T3645 T3643 conj calpains,caspases
R669 T3646 T3647 punct [,24
R670 T3647 T3640 parataxis 24,interplay
R671 T3746 T3745 punct -,calpain
R672 T3747 T3742 prep in,role
R673 T3648 T3647 nummod 23,24
R674 T3748 T3749 amod epidermal,factor
R675 T3749 T3751 npadvmod factor,induced
R676 T3750 T3749 compound growth,factor
R677 T3649 T3647 punct ",",24
R678 T3751 T3756 amod induced,motility
R679 T3752 T3749 punct (,factor
R680 T3650 T3647 punct ],24
R681 T3753 T3749 appos EGF,factor
R682 T3754 T3751 punct ),induced
R683 T3755 T3751 punct -,induced
R684 T3651 T3640 cc and,interplay
R685 T3756 T3747 pobj motility,in
R686 T3757 T3756 compound cell,motility
R687 T3758 T3759 punct [,29
R688 T3759 T3742 parataxis 29,role
R689 T3760 T3759 nummod 28,29
R690 T3761 T3759 punct ",",29
R691 T3652 T3640 conj impact,interplay
R692 T3762 T3759 punct ],29
R693 T3763 T3742 cc and,role
R694 T3764 T3765 det a,role
R695 T3653 T3652 prep of,impact
R696 T3765 T3742 conj role,role
R697 T3766 T3765 prep for,role
R698 T3654 T3653 pobj calpain,of
R699 T3767 T3768 compound μ,calpain
R700 T3768 T3766 pobj calpain,for
R701 T3769 T3768 punct -,calpain
R702 T3655 T3652 prep on,impact
R703 T3770 T3765 prep in,role
R704 T3771 T3772 npadvmod interferon,inducible
R705 T3772 T3774 amod inducible,migration
R706 T3656 T3657 compound cell,death
R707 T3773 T3772 punct -,inducible
R708 T3774 T3770 pobj migration,in
R709 T3775 T3776 npadvmod protein,induced
R710 T3657 T3658 compound death,components
R711 T3776 T3774 amod induced,migration
R712 T3777 T3775 nummod 9,protein
R713 T3778 T3776 punct -,induced
R714 T3658 T3655 pobj components,on
R715 T3779 T3774 prep of,migration
R716 T3780 T3779 pobj keratinocytes,of
R717 T3781 T3782 punct [,28
R718 T3659 T3658 compound pathway,components
R719 T3782 T3765 parataxis 28,role
R720 T3783 T3782 punct ],28
R721 T3784 T3733 punct .,suggested
R722 T3660 T3661 punct [,25
R723 T3786 T3787 det A,inhibitor
R724 T3661 T3652 parataxis 25,impact
R725 T3787 T3791 nsubjpass inhibitor,used
R726 T3788 T3789 npadvmod cell,permeable
R727 T3789 T3787 amod permeable,inhibitor
R728 T3790 T3787 compound calpain,inhibitor
R729 T3662 T3661 punct ],25
R730 T3792 T3787 punct (,inhibitor
R731 T3663 T3634 punct .,suggests
R732 T3793 T3794 dep which,inhibits
R733 T3794 T3787 relcl inhibits,inhibitor
R734 T3795 T3794 advmod likely,inhibits
R735 T3665 T3666 det The,lack
R736 T3796 T3797 amod other,proteases
R737 T3797 T3794 dobj proteases,inhibits
R738 T3798 T3797 compound thiol,proteases
R739 T3799 T3797 punct -,proteases
R740 T3800 T3801 advmod as,well
R741 T3801 T3794 advmod well,inhibits
R742 T3802 T3791 punct ),used
R743 T3666 T3667 nsubj lack,contributes
R744 T3803 T3791 aux has,used
R745 T3804 T3791 auxpass been,used
R746 T3805 T3806 aux to,select
R747 T3668 T3666 prep of,lack
R748 T3806 T3791 advcl select,used
R749 T3807 T3806 dobj cells,select
R750 T3808 T3807 acl lacking,cells
R751 T3809 T3810 compound μ,calpain
R752 T3669 T3670 advmod highly,specific
R753 T3670 T3671 amod specific,inhibitors
R754 T3810 T3808 dobj calpain,lacking
R755 T3671 T3668 pobj inhibitors,of
R756 T3811 T3810 punct -,calpain
R757 T3812 T3813 dep which,display
R758 T3813 T3807 relcl display,cells
R759 T3672 T3673 npadvmod cell,permeable
R760 T3814 T3815 amod reduced,rates
R761 T3815 T3813 dobj rates,display
R762 T3816 T3815 compound proliferation,rates
R763 T3673 T3671 amod permeable,inhibitors
R764 T3817 T3818 punct [,30
R765 T3818 T3813 parataxis 30,display
R766 T3819 T3818 punct ],30
R767 T3674 T3673 punct -,permeable
R768 T3820 T3791 punct .,used
R769 T3675 T3671 prep of,inhibitors
R770 T3822 T3823 advmod Interestingly,persisted
R771 T3676 T3675 pobj calpains,of
R772 T3824 T3823 punct ", ",persisted
R773 T3825 T3826 compound m,calpain
R774 T3826 T3828 compound calpain,expression
R775 T3677 T3667 prep to,contributes
R776 T3827 T3826 punct -,calpain
R777 T3828 T3823 nsubj expression,persisted
R778 T3829 T3823 prep in,persisted
R779 T3678 T3679 det the,challenge
R780 T3830 T3831 det these,cells
R781 T3831 T3829 pobj cells,in
R782 T3832 T3823 punct ", ",persisted
R783 T3679 T3677 pobj challenge,to
R784 T3833 T3823 advcl suggesting,persisted
R785 T3834 T3835 det a,requirement
R786 T3835 T3833 dobj requirement,suggesting
R787 T3680 T3679 prep of,challenge
R788 T3836 T3835 amod possible,requirement
R789 T3837 T3835 prep of,requirement
R790 T3681 T3680 pcomp investigating,of
R791 T3838 T3839 compound m,calpain
R792 T3839 T3837 pobj calpain,of
R793 T3840 T3839 punct -,calpain
R794 T3841 T3835 prep for,requirement
R795 T3842 T3843 compound cell,survival
R796 T3843 T3841 pobj survival,for
R797 T3682 T3681 cc and,investigating
R798 T3844 T3845 punct [,30
R799 T3845 T3823 parataxis 30,persisted
R800 T3846 T3845 punct ],30
R801 T3683 T3681 conj defining,investigating
R802 T3847 T3823 punct .,persisted
R803 T3684 T3685 compound calpain,functions
R804 T3849 T3850 amod Targeted,deletion
R805 T3685 T3683 dobj functions,defining
R806 T3686 T3683 prep in,defining
R807 T3850 T3852 nsubj deletion,provides
R808 T3687 T3688 det these,processes
R809 T3851 T3850 compound gene,deletion
R810 T3853 T3850 prep in,deletion
R811 T3688 T3686 pobj processes,in
R812 T3854 T3853 pobj mice,in
R813 T3855 T3856 det a,approach
R814 T3689 T3667 punct .,contributes
R815 T3856 T3852 dobj approach,provides
R816 T3691 T3692 mark Although,provides
R817 T3857 T3856 amod powerful,approach
R818 T3858 T3856 prep to,approach
R819 T3859 T3858 pcomp determining,to
R820 T3860 T3861 det the,roles
R821 T3692 T3708 advcl provides,distinguish
R822 T3861 T3859 dobj roles,determining
R823 T3862 T3861 amod physiological,roles
R824 T3863 T3861 prep of,roles
R825 T3693 T3692 nsubj over-expression,provides
R826 T3864 T3865 nmod μ,calpain
R827 T3865 T3863 pobj calpain,of
R828 T3694 T3693 prep of,over-expression
R829 T3866 T3864 punct -,μ
R830 T3867 T3864 cc and,μ
R831 T3868 T3864 conj m,μ
R832 T3695 T3694 pobj calpastatin,of
R833 T3869 T3865 punct -,calpain
R834 T3870 T3856 cc and,approach
R835 T3871 T3872 det the,opportunity
R836 T3696 T3695 punct ", ",calpastatin
R837 T3872 T3856 conj opportunity,approach
R838 T3873 T3874 aux to,approach
R839 T3697 T3698 det the,inhibitor
R840 T3874 T3872 acl approach,opportunity
R841 T3875 T3876 poss their,functions
R842 T3876 T3874 dobj functions,approach
R843 T3877 T3878 npadvmod isoform,specific
R844 T3878 T3876 amod specific,functions
R845 T3879 T3852 punct .,provides
R846 T3698 T3695 appos inhibitor,calpastatin
R847 T3881 T3882 amod Initial,studies
R848 T3882 T3883 nsubj studies,targeted
R849 T3699 T3698 amod endogenous,inhibitor
R850 T3884 T3883 dobj Capn4,targeted
R851 T3885 T3883 prep based,targeted
R852 T3700 T3698 compound protein,inhibitor
R853 T3886 T3885 prep on,based
R854 T3887 T3888 det the,prediction
R855 T3888 T3886 pobj prediction,on
R856 T3889 T3890 mark that,abolish
R857 T3701 T3698 prep of,inhibitor
R858 T3890 T3888 acl abolish,prediction
R859 T3702 T3703 nmod μ,calpain
R860 T3891 T3890 nsubj loss,abolish
R861 T3892 T3891 prep of,loss
R862 T3893 T3894 det this,subunit
R863 T3894 T3892 pobj subunit,of
R864 T3703 T3701 pobj calpain,of
R865 T3895 T3894 compound calpain,subunit
R866 T3896 T3890 aux would,abolish
R867 T3704 T3702 punct -,μ
R868 T3897 T3890 dobj activity,abolish
R869 T3705 T3702 cc and,μ
R870 T3898 T3897 prep of,activity
R871 T3899 T3900 preconj both,μ
R872 T3706 T3702 conj m,μ
R873 T3900 T3901 nmod μ,calpain
R874 T3901 T3898 pobj calpain,of
R875 T3707 T3703 punct -,calpain
R876 T3902 T3900 punct -,μ
R877 T3903 T3900 cc and,μ
R878 T3904 T3900 conj m,μ
R879 T3709 T3710 det an,approach
R880 T3905 T3901 punct -,calpain
R881 T3906 T3883 punct .,targeted
R882 T3908 T3909 nmod Capn4,embryos
R883 T3710 T3692 dobj approach,provides
R884 T3909 T3914 nsubj embryos,died
R885 T3910 T3908 punct -,Capn4
R886 T3911 T3908 punct /,Capn4
R887 T3711 T3710 amod important,approach
R888 T3912 T3908 punct -,Capn4
R889 T3913 T3909 amod murine,embryos
R890 T3712 T3710 prep for,approach
R891 T3915 T3914 prep between,died
R892 T3916 T3917 nmod days,10
R893 T3713 T3714 det these,efforts
R894 T3917 T3915 pobj 10,between
R895 T3918 T3917 cc and,10
R896 T3919 T3917 conj 11,10
R897 T3920 T3917 prep of,10
R898 T3921 T3920 pobj gestation,of
R899 T3922 T3914 punct ", ",died
R900 T3714 T3712 pobj efforts,for
R901 T3923 T3914 cc and,died
R902 T3924 T3925 expl there,was
R903 T3925 T3914 conj was,died
R904 T3715 T3708 punct ", ",distinguish
R905 T3926 T3927 det no,activity
R906 T3927 T3925 attr activity,was
R907 T3928 T3927 amod detectable,activity
R908 T3716 T3708 nsubj it,distinguish
R909 T3929 T3930 nmod μ,calpain
R910 T3930 T3927 compound calpain,activity
R911 T3931 T3929 punct -,μ
R912 T3717 T3708 aux will,distinguish
R913 T3932 T3929 cc or,μ
R914 T3933 T3929 conj m,μ
R915 T3718 T3708 neg not,distinguish
R916 T3934 T3930 punct -,calpain
R917 T3935 T3925 prep in,was
R918 T3936 T3935 pobj these,in
R919 T3937 T3936 cc or,these
R920 T3719 T3720 npadvmod isoform,specific
R921 T3938 T3939 amod younger,embryos
R922 T3939 T3936 conj embryos,these
R923 T3720 T3721 amod specific,functions
R924 T3940 T3941 punct [,31
R925 T3941 T3925 parataxis 31,was
R926 T3942 T3941 punct ],31
R927 T3721 T3708 dobj functions,distinguish
R928 T3943 T3925 punct .,was
R929 T3945 T3946 nsubjpass Capn4,cultured
R930 T3722 T3723 punct [,27
R931 T3947 T3945 punct -,Capn4
R932 T3948 T3945 punct /,Capn4
R933 T3723 T3708 parataxis 27,distinguish
R934 T3949 T3945 punct -,Capn4
R935 T3950 T3951 amod murine,fibroblasts
R936 T3724 T3723 nummod 24,27
R937 T3725 T3723 punct ",",27
R938 T3951 T3945 appos fibroblasts,Capn4
R939 T3726 T3723 nummod 26,27
R940 T3952 T3951 amod embryonic,fibroblasts
R941 T3727 T3723 punct ",",27
R942 T3953 T3951 punct (,fibroblasts
R943 T3954 T3951 appos MEFs,fibroblasts
R944 T3728 T3723 punct ],27
R945 T3729 T3708 punct .,distinguish
R946 T3955 T3946 punct ),cultured
R947 T3731 T3732 det Some,work
R948 T3956 T3946 aux could,cultured
R949 T3957 T3946 auxpass be,cultured
R950 T3958 T3946 prep from,cultured
R951 T3959 T3960 det these,embryos
R952 T3960 T3958 pobj embryos,from
R953 T3961 T3946 punct ", ",cultured
R954 T3962 T3963 mark although,lacked
R955 T3732 T3733 nsubj work,suggested
R956 T3963 T3946 advcl lacked,cultured
R957 T3964 T3963 nsubj they,lacked
R958 T3965 T3963 advmod also,lacked
R959 T3734 T3733 aux has,suggested
R960 T3966 T3967 compound calpain,activity
R961 T3967 T3963 dobj activity,lacked
R962 T3968 T3969 mark as,assessed
R963 T3969 T3963 advcl assessed,lacked
R964 T3735 T3736 npadvmod isoform,specific
R965 T3970 T3969 prep by,assessed
R966 T3971 T3972 compound casein,zymography
R967 T3972 T3970 pobj zymography,by
R968 T3736 T3737 amod specific,roles
R969 T3973 T3970 cc or,by
R970 T3974 T3970 conj by,by
R971 T3737 T3733 dobj roles,suggested
R972 T3975 T3976 det the,formation
R973 T3976 T3974 pobj formation,by
R974 T3738 T3737 punct ", ",roles
R975 T3977 T3976 prep of,formation
R976 T3978 T3979 amod characteristic,products
R977 T3979 T3977 pobj products,of
R978 T3739 T3740 amod such,as
R979 T3980 T3981 compound spectrin,breakdown
R980 T3981 T3979 compound breakdown,products
R981 T3740 T3737 prep as,roles
R982 T3741 T3742 det a,role
R983 T3982 T3963 punct ", ",lacked
R984 T3983 T3963 cc and,lacked
R985 T3742 T3740 pobj role,as
R986 T3984 T3985 nsubj they,displayed
R987 T3985 T3963 conj displayed,lacked
R988 T3986 T3987 compound migration,defects
R989 T3743 T3742 prep for,role
R990 T3987 T3985 dobj defects,displayed
R991 T3988 T3987 amod consistent,defects
R992 T3989 T3988 prep with,consistent
R993 T3744 T3745 compound m,calpain
R994 T3990 T3991 det a,role
R995 T3991 T3989 pobj role,with
R996 T3745 T3743 pobj calpain,for
R997 T3992 T3991 prep for,role
R998 T3993 T3992 pobj calpain,for
R999 T3994 T3991 prep in,role
R1000 T3995 T3994 pobj release,in
R1001 T3996 T3995 prep of,release
R1002 T3997 T3998 amod focal,adhesions
R1003 T4061 T4059 punct ", ",mutation
R1004 T3998 T3996 pobj adhesions,of
R1005 T3999 T4000 punct [,9
R1006 T4000 T3985 parataxis 9,displayed
R1007 T4001 T4000 punct ],9
R1008 T4002 T3946 punct .,cultured
R1009 T4062 T4063 dep which,retained
R1010 T4004 T4005 det An,knockout
R1011 T4005 T4009 nsubj knockout,resulted
R1012 T4063 T4059 relcl retained,mutation
R1013 T4006 T4007 advmod independently,derived
R1014 T4007 T4005 amod derived,knockout
R1015 T4008 T4005 compound Capn4,knockout
R1016 T4064 T4065 det some,level
R1017 T4010 T4005 punct ", ",knockout
R1018 T4011 T4005 prep involving,knockout
R1019 T4065 T4063 dobj level,retained
R1020 T4012 T4013 det a,deletion
R1021 T4013 T4011 pobj deletion,involving
R1022 T4014 T4015 advmod more,extensive
R1023 T4066 T4065 amod small,level
R1024 T4015 T4013 amod extensive,deletion
R1025 T4016 T4013 prep of,deletion
R1026 T4067 T4065 prep of,level
R1027 T4017 T4018 det the,gene
R1028 T4018 T4016 pobj gene,of
R1029 T4019 T4009 punct ", ",resulted
R1030 T4020 T4009 prep in,resulted
R1031 T4068 T4069 compound calpain,activity
R1032 T4021 T4022 det an,lethality
R1033 T4022 T4020 pobj lethality,in
R1034 T4069 T4067 pobj activity,of
R1035 T4023 T4022 amod earlier,lethality
R1036 T4024 T4022 amod embryonic,lethality
R1037 T4025 T4009 punct ", ",resulted
R1038 T4070 T4044 punct ", ",targeted
R1039 T4026 T4027 advmod apparently,at
R1040 T4027 T4009 prep at,resulted
R1041 T4028 T4029 det a,stage
R1042 T4029 T4027 pobj stage,at
R1043 T4071 T4044 advcl allowing,targeted
R1044 T4030 T4029 amod pre-implantation,stage
R1045 T4031 T4032 punct [,32
R1046 T4032 T4009 parataxis 32,resulted
R1047 T4072 T4071 prep for,allowing
R1048 T4033 T4032 punct ],32
R1049 T4034 T4009 punct .,resulted
R1050 T4073 T4074 poss their,survival
R1051 T4036 T4037 det The,times
R1052 T4037 T4039 nsubj times,suggested
R1053 T4038 T4037 amod different,times
R1054 T4040 T4037 prep of,times
R1055 T4074 T4072 pobj survival,for
R1056 T4041 T4042 amod embryonic,lethality
R1057 T4042 T4040 pobj lethality,of
R1058 T4043 T4044 mark that,targeted
R1060 T4044 T4039 ccomp targeted,suggested
R1061 T4045 T4046 det the,mice
R1062 T4046 T4044 nsubjpass mice,targeted
R1063 T4076 T4075 pobj mid-gestation,to
R1064 T4047 T4046 amod first,mice
R1065 T4048 T4046 amod reported,mice
R1066 T4077 T4044 punct ", ",targeted
R1067 T4078 T4079 mark while,represented
R1068 T4079 T4044 advcl represented,targeted
R1069 T4080 T4081 det the,mice
R1071 T4082 T4081 amod second,mice
R1072 T4083 T4081 amod reported,mice
R1073 T4084 T4081 nmod Capn4,mice
R1074 T4085 T4084 punct -,Capn4
R1075 T4086 T4084 punct /,Capn4
R1076 T4087 T4084 punct -,Capn4
R1077 T4088 T4089 punct [,32
R1078 T4049 T4046 nmod Capn4,mice
R1079 T4089 T4081 parataxis 32,mice
R1080 T4090 T4089 punct ],32
R1081 T4050 T4049 punct -,Capn4
R1082 T4091 T4092 det a,mutation
R1083 T4051 T4049 punct /,Capn4
R1084 T4052 T4049 punct -,Capn4
R1085 T4092 T4079 dobj mutation,represented
R1086 T4053 T4054 punct [,31
R1087 T4054 T4046 parataxis 31,mice
R1088 T4093 T4092 amod true,mutation
R1089 T4055 T4054 punct ],31
R1090 T4094 T4092 amod null,mutation
R1091 T4056 T4044 auxpass were,targeted
R1092 T4057 T4044 prep with,targeted
R1093 T4058 T4059 det a,mutation
R1094 T4095 T4039 punct .,suggested
R1095 T4059 T4057 pobj mutation,with
R1096 T4060 T4059 amod hypomorphic,mutation
R1097 T4097 T4098 nsubjpass Disruption,reported
R1098 T4099 T4097 prep of,Disruption
R1099 T4100 T4099 pobj Capn1,of
R1100 T4101 T4097 punct ", ",Disruption
R1101 T4102 T4097 acl encoding,Disruption
R1102 T4168 T4169 det The,viability
R1103 T4169 T4170 nsubj viability,distinguish
R1104 T4171 T4169 prep of,viability
R1105 T4172 T4173 npadvmod Capn1,deficient
R1106 T4173 T4175 amod deficient,mice
R1107 T4174 T4173 punct -,deficient
R1108 T4103 T4104 det the,subunit
R1109 T4175 T4171 pobj mice,of
R1110 T4176 T4170 aux does,distinguish
R1111 T4177 T4170 neg not,distinguish
R1112 T4104 T4102 dobj subunit,encoding
R1113 T4178 T4170 advmod however,distinguish
R1114 T4179 T4170 prep between,distinguish
R1115 T4180 T4181 nummod two,possibilities
R1116 T4105 T4106 nmod μ,calpain
R1117 T4181 T4179 pobj possibilities,between
R1118 T4182 T4181 punct : ,possibilities
R1119 T4106 T4104 nmod calpain,subunit
R1120 T4183 T4184 preconj either,required
R1121 T4184 T4181 advcl required,possibilities
R1122 T4185 T4184 mark that,required
R1123 T4107 T4106 punct -,calpain
R1124 T4186 T4187 compound m,calpain
R1125 T4187 T4184 nsubjpass calpain,required
R1126 T4188 T4187 punct -,calpain
R1127 T4108 T4104 amod catalytic,subunit
R1128 T4189 T4184 auxpass is,required
R1129 T4190 T4184 advmod specifically,required
R1130 T4109 T4098 punct ", ",reported
R1131 T4191 T4184 prep during,required
R1132 T4192 T4191 pobj embryogenesis,during
R1133 T4193 T4184 punct ", ",required
R1134 T4110 T4098 auxpass was,reported
R1135 T4194 T4184 cc or,required
R1136 T4195 T4196 mark that,is
R1137 T4196 T4184 conj is,required
R1138 T4111 T4098 advmod subsequently,reported
R1139 T4197 T4198 preconj either,form
R1140 T4198 T4196 nsubj form,is
R1141 T4199 T4198 prep of,form
R1142 T4112 T4113 aux to,result
R1143 T4200 T4199 pobj calpain,of
R1144 T4201 T4198 advmod alone,form
R1145 T4202 T4196 acomp sufficient,is
R1146 T4113 T4098 xcomp result,reported
R1147 T4203 T4196 cc and,is
R1148 T4204 T4205 aux can,compensate
R1149 T4114 T4113 prep in,result
R1150 T4205 T4196 conj compensate,is
R1151 T4206 T4205 prep for,compensate
R1152 T4207 T4208 det the,absence
R1153 T4115 T4116 amod fertile,mice
R1154 T4208 T4206 pobj absence,for
R1155 T4209 T4208 prep of,absence
R1156 T4210 T4211 det the,other
R1157 T4211 T4209 pobj other,of
R1158 T4212 T4170 punct .,distinguish
R1159 T4116 T4114 pobj mice,in
R1160 T4214 T4215 aux To,resolve
R1161 T4215 T4216 advcl resolve,knocked
R1162 T4117 T4116 punct ", ",mice
R1163 T4217 T4218 det this,question
R1164 T4218 T4215 dobj question,resolve
R1165 T4118 T4116 amod viable,mice
R1166 T4219 T4216 punct ", ",knocked
R1167 T4220 T4216 nsubj we,knocked
R1168 T4221 T4216 aux have,knocked
R1169 T4119 T4116 prep with,mice
R1170 T4222 T4216 advmod now,knocked
R1171 T4223 T4216 prt out,knocked
R1172 T4224 T4225 det the,gene
R1173 T4120 T4121 det some,defects
R1174 T4225 T4216 dobj gene,knocked
R1175 T4226 T4225 compound Capn2,gene
R1176 T4227 T4225 acl encoding,gene
R1177 T4121 T4119 pobj defects,with
R1178 T4228 T4229 det the,subunit
R1179 T4229 T4227 dobj subunit,encoding
R1180 T4230 T4231 nmod m,k
R1181 T4122 T4121 amod mild,defects
R1182 T4231 T4229 compound k,subunit
R1183 T4232 T4231 punct -,k
R1184 T4233 T4231 nummod 80,k
R1185 T4123 T4121 prep in,defects
R1186 T4234 T4227 prep in,encoding
R1187 T4124 T4125 det the,platelets
R1188 T4125 T4123 pobj platelets,in
R1189 T4126 T4127 compound μ,calpain
R1190 T4235 T4234 pobj mice,in
R1191 T4236 T4216 punct .,knocked
R1192 T4127 T4129 npadvmod calpain,rich
R1193 T4238 T4239 nsubj We,report
R1194 T4128 T4127 punct -,calpain
R1195 T4240 T4239 advmod here,report
R1196 T4241 T4242 mark that,died
R1197 T4242 T4239 ccomp died,report
R1198 T4129 T4125 amod rich,platelets
R1199 T4243 T4244 npadvmod Capn2,null
R1200 T4244 T4245 amod null,embryos
R1201 T4130 T4121 acl relating,defects
R1202 T4245 T4242 nsubj embryos,died
R1203 T4246 T4247 amod prior,to
R1204 T4131 T4130 prep to,relating
R1205 T4247 T4242 prep to,died
R1206 T4248 T4249 det the,stage
R1207 T4249 T4247 pobj stage,to
R1208 T4250 T4249 compound implantation,stage
R1209 T4251 T4242 punct ", ",died
R1210 T4252 T4242 advcl indicating,died
R1211 T4132 T4133 poss their,aggregation
R1212 T4253 T4254 mark that,is
R1213 T4254 T4252 ccomp is,indicating
R1214 T4255 T4256 compound m,calpain
R1215 T4133 T4131 pobj aggregation,to
R1216 T4256 T4254 nsubj calpain,is
R1217 T4257 T4256 punct -,calpain
R1218 T4258 T4254 acomp indispensable,is
R1219 T4134 T4133 cc and,aggregation
R1220 T4259 T4258 prep for,indispensable
R1221 T4260 T4261 amod early,embryogenesis
R1222 T4261 T4259 pobj embryogenesis,for
R1223 T4135 T4136 compound clot,retraction
R1224 T4262 T4239 punct .,report
R1225 T4264 T4265 det This,role
R1226 T4136 T4133 conj retraction,aggregation
R1227 T4265 T4266 nsubjpass role,fulfilled
R1228 T4267 T4266 aux can,fulfilled
R1229 T4137 T4138 punct [,33
R1230 T4268 T4266 neg not,fulfilled
R1231 T4269 T4266 auxpass be,fulfilled
R1232 T4270 T4266 agent by,fulfilled
R1233 T4138 T4098 parataxis 33,reported
R1234 T4271 T4272 compound μ,calpain
R1235 T4272 T4270 pobj calpain,by
R1236 T4139 T4138 punct ],33
R1237 T4140 T4098 punct .,reported
R1238 T4142 T4143 det The,fact
R1239 T4273 T4272 punct -,calpain
R1240 T4274 T4272 punct ", ",calpain
R1241 T4275 T4276 dep which,expressed
R1242 T4143 T4144 nsubj fact,indicates
R1243 T4276 T4272 relcl expressed,calpain
R1244 T4277 T4276 auxpass is,expressed
R1245 T4278 T4276 prep in,expressed
R1246 T4145 T4146 mark that,die
R1247 T4279 T4280 amod embryonic,stem
R1248 T4280 T4281 nmod stem,cells
R1249 T4281 T4278 pobj cells,in
R1250 T4282 T4280 punct (,stem
R1251 T4146 T4143 acl die,fact
R1252 T4283 T4280 appos ES,stem
R1253 T4284 T4281 punct ),cells
R1254 T4285 T4286 punct [,31
R1255 T4147 T4148 npadvmod Capn4,null
R1256 T4286 T4276 parataxis 31,expressed
R1257 T4287 T4286 punct ],31
R1258 T4288 T4276 cc and,expressed
R1259 T4148 T4149 amod null,mice
R1260 T4289 T4290 auxpass is,assumed
R1261 T4290 T4276 conj assumed,expressed
R1262 T4291 T4292 aux to,be
R1263 T4292 T4290 xcomp be,assumed
R1264 T4293 T4292 acomp present,be
R1265 T4294 T4293 prep at,present
R1266 T4149 T4146 nsubj mice,die
R1270 T4150 T4146 prep during,die
R1271 T4298 T4297 pobj gestation,of
R1272 T4299 T4266 punct .,fulfilled
R1273 T4151 T4150 pobj embryogenesis,during
R1274 T4301 T4302 nsubj This,demonstrates
R1275 T4152 T4153 mark that,is
R1276 T4303 T4302 advmod unequivocally,demonstrates
R1277 T4304 T4305 mark that,have
R1278 T4305 T4302 ccomp have,demonstrates
R1279 T4153 T4144 ccomp is,indicates
R1280 T4306 T4307 compound m,calpain
R1281 T4307 T4305 nsubj calpain,have
R1282 T4308 T4307 punct -,calpain
R1283 T4154 T4155 advmod at,one
R1284 T4309 T4307 cc and,calpain
R1285 T4310 T4311 compound μ,calpain
R1286 T4311 T4307 conj calpain,calpain
R1287 T4155 T4153 nsubj one,is
R1288 T4312 T4311 punct -,calpain
R1289 T4313 T4314 amod distinct,roles
R1290 T4314 T4305 dobj roles,have
R1291 T4156 T4155 advmod least,one
R1292 T4315 T4314 amod physiological,roles
R1293 T4316 T4305 prep during,have
R1294 T4317 T4318 amod early,embryogenesis
R1295 T4157 T4155 prep of,one
R1296 T4318 T4316 pobj embryogenesis,during
R1297 T4319 T4302 punct .,demonstrates
R1298 T4158 T4159 det the,calpains
R1299 T4159 T4157 pobj calpains,of
R1300 T4160 T4159 amod ubiquitous,calpains
R1301 T4161 T4153 acomp essential,is
R1302 T4162 T4161 prep for,essential
R1303 T4163 T4162 pobj development,for
R1304 T4164 T4163 prep to,development
R1305 T4165 T4164 pobj term,to
R1306 T4166 T4144 punct .,indicates
R576 T3568 T3569 amod neuronal,growth
R577 T3569 T3563 conj growth,degeneration
R1059 T4075 T4074 prep to,survival
R1070 T4081 T4079 nsubj mice,represented
R1267 T4295 T4296 det this,stage
R1268 T4296 T4294 pobj stage,at
R1269 T4297 T4296 prep of,stage

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2933 152-156 SO:0000704 denotes gene
T2934 178-183 SO:0000704 denotes genes
T2935 187-194 NCBITaxon:40674 denotes mammals
T2936 298-303 PR:000005009 denotes Capn1
T2937 305-311 PR:000005009 denotes μ-80 k
T2938 317-322 PR:000005015 denotes Capn2
T2939 324-330 PR:000005015 denotes m-80 k
T2940 332-337 SO:0000704 denotes genes
T2941 389-399 GO:0065007 denotes regulatory
T2942 423-428 PR:000005022 denotes Capn4
T2943 429-433 SO:0000704 denotes gene
T2944 439-445 _FRAGMENT denotes μ-80 k
T2945 457-465 PR:000005009 denotes subunits
T2946 450-465 PR:000005015 denotes m-80 k subunits
T2947 650-660 GO:0010467 denotes expression
T2948 693-702 NCBITaxon:40674 denotes mammalian
T2949 703-710 UBERON:0000479 denotes tissues
T2950 734-741 SO:0001060 denotes isoform
T2951 826-830 CHEBI:29108 denotes Ca2+
T2952 882-886 CHEBI:29108 denotes Ca2+
T2953 917-921 CHEBI:29108 denotes Ca2+
T2954 1122-1133 GO:0005737 denotes cytoplasmic
T2955 1139-1143 CHEBI:29108 denotes Ca2+
T2956 1252-1262 GO:0065007 denotes regulation
T2957 1326-1336 GO:0065007 denotes regulating
T2958 1581-1589 CL:0000540 denotes neuronal
T2959 1624-1634 GO:0007049 denotes cell cycle
T2960 1717-1725 GO:0016020 denotes membrane
T2961 1760-1772 GO:0005856 denotes cytoskeleton
T2962 1777-1791 GO:0016477 denotes cell migration
T2963 1804-1828 GO:0010941 denotes regulation of cell death
T2964 1851-1860 GO:0006915 denotes apoptosis
T2965 1986-1996 GO:0008219 denotes cell death
T2966 2065-2087 CHEBI:82824 denotes inhibitors of calpains
T2967 2199-2209 GO:0010467 denotes expression
T2968 2213-2224 PR:000005060 denotes calpastatin
T2969 2249-2261 _FRAGMENT denotes inhibitor of
T2970 2271-2278 CHEBI:82824 denotes calpain
T2971 2353-2360 SO:0001060 denotes isoform
T2972 2416-2423 SO:0001060 denotes isoform
T2973 2472-2481 UBERON:0007376 denotes epidermal
T2974 2472-2495 PR:000006928 denotes epidermal growth factor
T2975 2497-2500 PR:000006928 denotes EGF
T2976 2510-2523 GO:0048870 denotes cell motility
T2977 2560-2590 PR:000006065 denotes interferon-inducible protein 9
T2978 2599-2625 GO:0051546 denotes migration of keratinocytes
T2979 2612-2625 CL:0000312 denotes keratinocytes
T2980 2649-2666 CHEBI:82824 denotes calpain inhibitor
T2981 2696-2701 CHEBI:29917 denotes thiol
T2982 2791-2804 GO:0008283 denotes proliferation
T2983 2842-2852 GO:0010467 denotes expression
T2984 2959-2963 SO:0000704 denotes gene
T2985 2976-2980 NCBITaxon:10088 denotes mice
T2986 3107-3114 SO:0001060 denotes isoform
T2987 3160-3165 PR:000005022 denotes Capn4
T2988 3273-3278 PR:000005022 denotes Capn4
T2989 3282-3288 NCBITaxon:39107 denotes murine
T2990 3289-3296 UBERON:0000922 denotes embryos
T2991 3297-3301 GO:0016265 denotes died
T2992 3328-3337 GO:0007565 denotes gestation
T2993 3412-3419 UBERON:0000922 denotes embryos
T2994 3426-3431 PR:000005022 denotes Capn4
T2995 3435-3441 NCBITaxon:39107 denotes murine
T2996 3442-3451 UBERON:0000922 denotes embryonic
T2997 3452-3463 CL:0000057 denotes fibroblasts
T2998 3500-3507 UBERON:0000922 denotes embryos
T2999 3738-3753 GO:0005925 denotes focal adhesions
T3000 3784-3789 PR:000005022 denotes Capn4
T3001 3843-3847 SO:0000704 denotes gene
T3002 3872-3881 UBERON:0000922 denotes embryonic
T3003 3913-3925 GO:0007566 denotes implantation
T3004 3961-3970 UBERON:0000922 denotes embryonic
T3005 4015-4020 PR:000005022 denotes Capn4
T3006 4024-4028 NCBITaxon:10088 denotes mice
T3007 4165-4174 GO:0007565 denotes gestation
T3008 4202-4207 PR:000005022 denotes Capn4
T3009 4211-4215 NCBITaxon:10088 denotes mice
T3010 4269-4274 PR:000005009 denotes Capn1
T3011 4289-4316 PR:000005009 denotes μ-calpain catalytic subunit
T3012 4373-4377 NCBITaxon:10088 denotes mice
T3013 4423-4432 CL:0000233 denotes platelets
T3014 4503-4508 PR:000005022 denotes Capn4
T3015 4514-4518 NCBITaxon:10088 denotes mice
T3016 4519-4522 GO:0016265 denotes die
T3017 4530-4543 GO:0009790 denotes embryogenesis
T3018 4654-4659 PR:000005009 denotes Capn1
T3019 4670-4674 NCBITaxon:10088 denotes mice
T3020 4785-4798 GO:0009790 denotes embryogenesis
T3021 4954-4959 PR:000005015 denotes Capn2
T3022 4960-4964 SO:0000704 denotes gene
T3023 4978-4992 PR:000005015 denotes m-80 k subunit
T3024 4996-5000 NCBITaxon:10088 denotes mice
T3025 5022-5027 PR:000005015 denotes Capn2
T3026 5033-5040 UBERON:0000922 denotes embryos
T3027 5041-5045 GO:0016265 denotes died
T3028 5059-5071 GO:0007566 denotes implantation
T3029 5132-5145 GO:0009790 denotes embryogenesis
T3030 5200-5209 GO:0010467 denotes expressed
T3031 5213-5222 UBERON:0000922 denotes embryonic
T3032 5213-5227 _FRAGMENT denotes embryonic stem
T3033 5233-5238 CL:0002322 denotes cells
T3034 5229-5231 _FRAGMENT denotes ES
T3035 5290-5299 GO:0007565 denotes gestation
T3036 5409-5422 GO:0009790 denotes embryogenesis
T2931 30-34 CHEBI:29108 denotes Ca2+
T2932 111-119 PR:000005015 denotes capain-2
R271 T2945 T2944 _lexicallyChainedTo subunits,μ-80 k
R272 T2970 T2969 _lexicallyChainedTo calpain,inhibitor of
R273 T3033 T3032 _lexicallyChainedTo cells,embryonic stem
R276 T3033 T3034 _lexicallyChainedTo cells,ES

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T3038 46-54 CHEBI_SO_EXT:cysteine denotes cysteine
T3039 46-64 GO_EXT:0008234 denotes cysteine proteases
T3040 74-83 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3041 85-94 GO_PR_EXT:calpain_1_complex denotes calpain-1
T3042 100-109 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3043 111-119 PR_EXT:000005015 denotes capain-2
T3044 111-119 GO_PR_EXT:calpain_2_complex denotes capain-2
T3045 152-156 SO_EXT:0000704 denotes gene
T3046 178-183 SO_EXT:0000704 denotes genes
T3047 187-194 NCBITaxon:40674 denotes mammals
T3048 217-224 GO_EXT:dimer_macromolecular_complex denotes dimeric
T3049 225-232 CHEBI_GO_SO_EXT:enzyme denotes enzymes
T3050 263-272 GO_MOP_EXT:catalysis denotes catalytic
T3051 283-290 SO_EXT:sequence_coding_function denotes encoded
T3052 298-303 PR_EXT:000005009 denotes Capn1
T3053 305-311 PR_EXT:000005009 denotes μ-80 k
T3054 317-322 PR_EXT:000005015 denotes Capn2
T3055 324-330 PR_EXT:000005015 denotes m-80 k
T3056 332-337 SO_EXT:0000704 denotes genes
T3057 389-399 GO:0065007 denotes regulatory
T3058 408-415 SO_EXT:sequence_coding_function denotes encoded
T3059 423-428 PR_EXT:000005022 denotes Capn4
T3060 429-433 SO_EXT:0000704 denotes gene
T3061 439-445 _FRAGMENT denotes μ-80 k
T3062 457-465 PR_EXT:000005009 denotes subunits
T3063 450-465 PR_EXT:000005015 denotes m-80 k subunits
T3064 476-486 CHEBI_SO_EXT:amino_acid denotes amino acid
T3065 487-495 SO_EXT:biological_sequence denotes sequence
T3066 550-557 CHEBI_PR_EXT:protein denotes protein
T3067 650-660 GO:0010467 denotes expression
T3068 673-675 _FRAGMENT denotes μ-
T3069 682-689 GO_PR_EXT:calpain_1_complex denotes calpain
T3070 680-689 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3071 693-702 NCBITaxon:40674 denotes mammalian
T3072 703-710 UBERON:0000479 denotes tissues
T3073 734-741 SO_EXT:0001060 denotes isoform
T3074 826-830 CHEBI:29108 denotes Ca2+
T3075 882-886 CHEBI:29108 denotes Ca2+
T3076 891-900 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3077 917-921 CHEBI:29108 denotes Ca2+
T3078 926-935 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3079 966-968 _FRAGMENT denotes μ-
T3080 975-982 GO_PR_EXT:calpain_1_complex denotes calpain
T3081 973-982 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3082 1028-1035 CHEBI_EXT:calcium denotes calcium
T3083 1122-1133 GO:0005737 denotes cytoplasmic
T3084 1139-1143 CHEBI:29108 denotes Ca2+
T3085 1252-1262 GO:0065007 denotes regulation
T3086 1272-1279 CHEBI_GO_SO_EXT:enzyme denotes enzymes
T3087 1326-1336 GO:0065007 denotes regulating
T3088 1399-1409 CHEBI_GO_EXT:biological_process_or_quality_or_role denotes biological
T3089 1535-1541 UBERON_EXT:muscle_structure_or_tissue denotes muscle
T3090 1535-1541 _FRAGMENT denotes muscle
T3091 1550-1561 GO_EXT:muscle_structure_or_tissue_development denotes development
T3092 1542-1548 GO_EXT:biological_growth_entity_or_process denotes growth
T3093 1581-1589 CL:0000540 denotes neuronal
T3094 1590-1596 GO_EXT:biological_growth_entity_or_process denotes growth
T3095 1624-1628 CL_GO_EXT:cell denotes cell
T3096 1624-1634 GO:0007049 denotes cell cycle
T3097 1654-1669 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signal cascades
T3098 1683-1692 GO_PR_EXT:integrin denotes integrins
T3099 1697-1703 GO_EXT:biological_growth_entity_or_process denotes growth
T3100 1697-1711 GO_EXT:0008083 denotes growth factors
T3101 1717-1725 GO:0016020 denotes membrane
T3102 1760-1772 GO:0005856 denotes cytoskeleton
T3103 1777-1781 CL_GO_EXT:cell denotes cell
T3104 1777-1791 GO:0016477 denotes cell migration
T3105 1804-1828 GO:0010941 denotes regulation of cell death
T3106 1818-1822 CL_GO_EXT:cell denotes cell
T3107 1838-1846 GO_PATO_EXT:necrosis denotes necrosis
T3108 1851-1860 GO:0006915 denotes apoptosis
T3109 1986-1990 CL_GO_EXT:cell denotes cell
T3110 1986-1996 GO:0008219 denotes cell death
T3111 2050-2054 CL_GO_EXT:cell denotes cell
T3112 2065-2087 CHEBI_EXT:82824 denotes inhibitors of calpains
T3113 2199-2209 GO:0010467 denotes expression
T3114 2213-2224 PR_EXT:000005060 denotes calpastatin
T3115 2241-2248 CHEBI_PR_EXT:protein denotes protein
T3116 2249-2261 _FRAGMENT denotes inhibitor of
T3117 2271-2278 CHEBI_EXT:82824 denotes calpain
T3118 2262-2264 _FRAGMENT denotes μ-
T3119 2271-2278 GO_PR_EXT:calpain_1_complex denotes calpain
T3120 2269-2278 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3121 2353-2360 SO_EXT:0001060 denotes isoform
T3122 2416-2423 SO_EXT:0001060 denotes isoform
T3123 2459-2468 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3124 2472-2481 UBERON:0007376 denotes epidermal
T3125 2472-2495 PR_EXT:000006928 denotes epidermal growth factor
T3126 2482-2488 GO_EXT:biological_growth_entity_or_process denotes growth
T3127 2482-2495 GO_EXT:0008083 denotes growth factor
T3128 2497-2500 PR_EXT:000006928 denotes EGF
T3129 2510-2514 CL_GO_EXT:cell denotes cell
T3130 2510-2523 GO:0048870 denotes cell motility
T3131 2547-2556 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3132 2560-2590 PR_EXT:000006065 denotes interferon-inducible protein 9
T3133 2581-2588 CHEBI_PR_EXT:protein denotes protein
T3134 2599-2625 GO:0051546 denotes migration of keratinocytes
T3135 2612-2625 CL:0000312 denotes keratinocytes
T3136 2634-2638 CL_GO_EXT:cell denotes cell
T3137 2649-2666 CHEBI_EXT:82824 denotes calpain inhibitor
T3138 2696-2701 CHEBI:29917 denotes thiol
T3139 2696-2711 GO_EXT:0008234 denotes thiol-proteases
T3140 2745-2750 CL_GO_EXT:cell denotes cells
T3141 2759-2768 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3142 2791-2804 GO:0008283 denotes proliferation
T3143 2832-2841 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3144 2842-2852 GO:0010467 denotes expression
T3145 2872-2877 CL_GO_EXT:cell denotes cells
T3146 2916-2925 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3147 2930-2934 CL_GO_EXT:cell denotes cell
T3148 2959-2963 SO_EXT:0000704 denotes gene
T3149 2964-2972 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T3150 2976-2980 NCBITaxon:10088 denotes mice
T3151 3029-3042 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T3152 3052-3054 _FRAGMENT denotes μ-
T3153 3061-3068 GO_PR_EXT:calpain_1_complex denotes calpain
T3154 3059-3068 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3155 3107-3114 SO_EXT:0001060 denotes isoform
T3156 3160-3165 PR_EXT:000005022 denotes Capn4
T3157 3255-3257 _FRAGMENT denotes μ-
T3158 3264-3271 GO_PR_EXT:calpain_1_complex denotes calpain
T3159 3262-3271 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3160 3273-3278 PR_EXT:000005022 denotes Capn4
T3161 3278-3279 SO_EXT:sequence_nullness_or_absence denotes -
T3162 3280-3281 SO_EXT:sequence_nullness_or_absence denotes -
T3163 3282-3288 NCBITaxon:39107 denotes murine
T3164 3289-3296 UBERON:0000922 denotes embryos
T3165 3297-3301 GO:0016265 denotes died
T3166 3328-3337 GO:0007565 denotes gestation
T3167 3367-3369 _FRAGMENT denotes μ-
T3168 3375-3382 GO_PR_EXT:calpain_1_complex denotes calpain
T3169 3373-3382 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3170 3412-3419 UBERON:0000922 denotes embryos
T3171 3426-3431 PR_EXT:000005022 denotes Capn4
T3172 3431-3432 SO_EXT:sequence_nullness_or_absence denotes -
T3173 3433-3434 SO_EXT:sequence_nullness_or_absence denotes -
T3174 3435-3441 NCBITaxon:39107 denotes murine
T3175 3442-3451 UBERON:0000922 denotes embryonic
T3176 3452-3463 CL:0000057 denotes fibroblasts
T3177 3500-3507 UBERON:0000922 denotes embryos
T3178 3623-3631 GO_PR_EXT:spectrin denotes spectrin
T3179 3671-3680 GO_EXT:biological_movement_or_translocation_process denotes migration
T3180 3727-3734 GO_EXT:biological_release denotes release
T3181 3738-3753 GO:0005925 denotes focal adhesions
T3182 3744-3753 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesions
T3183 3784-3789 PR_EXT:000005022 denotes Capn4
T3184 3827-3835 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T3185 3843-3847 SO_EXT:0000704 denotes gene
T3186 3872-3881 UBERON:0000922 denotes embryonic
T3187 3882-3891 GO_EXT:fatality_or_lethality denotes lethality
T3188 3913-3925 GO:0007566 denotes implantation
T3189 3961-3970 UBERON:0000922 denotes embryonic
T3190 3971-3980 GO_EXT:fatality_or_lethality denotes lethality
T3191 4015-4020 PR_EXT:000005022 denotes Capn4
T3192 4020-4021 SO_EXT:sequence_nullness_or_absence denotes -
T3193 4022-4023 SO_EXT:sequence_nullness_or_absence denotes -
T3194 4024-4028 NCBITaxon:10088 denotes mice
T3195 4067-4075 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T3196 4165-4174 GO:0007565 denotes gestation
T3197 4202-4207 PR_EXT:000005022 denotes Capn4
T3198 4207-4208 SO_EXT:sequence_nullness_or_absence denotes -
T3199 4209-4210 SO_EXT:sequence_nullness_or_absence denotes -
T3200 4211-4215 NCBITaxon:10088 denotes mice
T3201 4240-4244 SO_EXT:sequence_nullness denotes null
T3202 4245-4253 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T3203 4269-4274 PR_EXT:000005009 denotes Capn1
T3204 4276-4284 SO_EXT:sequence_coding_function denotes encoding
T3205 4289-4298 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3206 4289-4316 PR_EXT:000005009 denotes μ-calpain catalytic subunit
T3207 4299-4308 GO_MOP_EXT:catalysis denotes catalytic
T3208 4373-4377 NCBITaxon:10088 denotes mice
T3209 4408-4417 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3210 4423-4432 CL:0000233 denotes platelets
T3211 4503-4508 PR_EXT:000005022 denotes Capn4
T3212 4509-4513 SO_EXT:sequence_nullness denotes null
T3213 4514-4518 NCBITaxon:10088 denotes mice
T3214 4519-4522 GO:0016265 denotes die
T3215 4530-4543 GO:0009790 denotes embryogenesis
T3216 4654-4659 PR_EXT:000005009 denotes Capn1
T3217 4670-4674 NCBITaxon:10088 denotes mice
T3218 4743-4752 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3219 4785-4798 GO:0009790 denotes embryogenesis
T3220 4954-4959 PR_EXT:000005015 denotes Capn2
T3221 4960-4964 SO_EXT:0000704 denotes gene
T3222 4965-4973 SO_EXT:sequence_coding_function denotes encoding
T3223 4978-4992 PR_EXT:000005015 denotes m-80 k subunit
T3224 4996-5000 NCBITaxon:10088 denotes mice
T3225 5022-5027 PR_EXT:000005015 denotes Capn2
T3226 5028-5032 SO_EXT:sequence_nullness denotes null
T3227 5033-5040 UBERON:0000922 denotes embryos
T3228 5041-5045 GO:0016265 denotes died
T3229 5059-5071 GO:0007566 denotes implantation
T3230 5095-5104 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3231 5132-5145 GO:0009790 denotes embryogenesis
T3232 5180-5189 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3233 5200-5209 GO:0010467 denotes expressed
T3234 5213-5222 UBERON:0000922 denotes embryonic
T3235 5213-5227 _FRAGMENT denotes embryonic stem
T3236 5233-5238 CL:0002322 denotes cells
T3237 5229-5231 _FRAGMENT denotes ES
T3238 5233-5238 CL_GO_EXT:cell denotes cells
T3239 5290-5299 GO:0007565 denotes gestation
T3240 5338-5347 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3241 5352-5361 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3242 5376-5389 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T3243 5409-5422 GO:0009790 denotes embryogenesis
T3037 30-34 CHEBI:29108 denotes Ca2+
R274 T3062 T3061 _lexicallyChainedTo subunits,μ-80 k
R275 T3069 T3068 _lexicallyChainedTo calpain,μ-
R277 T3153 T3152 _lexicallyChainedTo calpain,μ-
R278 T3080 T3079 _lexicallyChainedTo calpain,μ-
R279 T3091 T3090 _lexicallyChainedTo development,muscle
R280 T3158 T3157 _lexicallyChainedTo calpain,μ-
R281 T3168 T3167 _lexicallyChainedTo calpain,μ-
R283 T3117 T3116 _lexicallyChainedTo calpain,inhibitor of
R284 T3119 T3118 _lexicallyChainedTo calpain,μ-
R285 T3236 T3235 _lexicallyChainedTo cells,embryonic stem
R286 T3236 T3237 _lexicallyChainedTo cells,ES