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Id Subject Object Predicate Lexical cue
T23649 31106-31107 . denotes .
T23648 31098-31106 NNS denotes purposes
T23647 31087-31097 NN denotes genotyping
T23646 31083-31086 IN denotes for
T23645 31078-31082 VBN denotes used
T23644 31073-31077 RB denotes also
T23643 31065-31072 NNS denotes primers
T23642 31061-31064 NN denotes PCR
T23641 31058-31060 IN denotes of
T23640 31048-31057 NNS denotes positions
T23639 31044-31047 DT denotes the
T23638 31029-31038 NNS denotes triangles
T23637 31039-31043 VBP denotes mark
T23636 31023-31028 IN denotes while
T23635 31021-31023 , denotes ,
T23634 31001-31011 JJ denotes horizontal
T23633 30999-31001 , denotes ,
T23632 30994-30999 JJ denotes solid
T23631 31012-31021 NN denotes rectangle
T23630 30992-30993 DT denotes a
T23629 30989-30991 IN denotes as
T23628 30980-30982 VBZ denotes is
T23627 30966-30970 NN denotes blot
T23626 30957-30965 NNP denotes Southern
T23625 30971-30979 NNS denotes analyses
T23624 30952-30956 JJS denotes most
T23623 30949-30951 IN denotes in
T23622 30944-30948 VBN denotes used
T23621 30983-30988 VBN denotes shown
T23620 30938-30943 NN denotes probe
T23619 30934-30937 DT denotes The
T23618 30933-31107 sentence denotes The probe used in most Southern blot analyses is shown as a solid, horizontal rectangle, while triangles mark the positions of PCR primers also used for genotyping purposes.
T23617 30932-30933 . denotes .
T23616 30914-30922 JJ denotes vertical
T23615 30908-30913 JJ denotes solid
T23614 30923-30932 NN denotes rectangle
T23613 30906-30907 DT denotes a
T23612 30903-30905 IN denotes by
T23611 30888-30890 VBZ denotes is
T23610 30891-30902 VBN denotes represented
T23609 30882-30887 WDT denotes which
T23608 30880-30881 CD denotes 7
T23607 30875-30879 NN denotes exon
T23606 30871-30874 IN denotes for
T23605 30864-30870 IN denotes except
T23604 30844-30852 JJ denotes vertical
T23603 30853-30863 NNS denotes rectangles
T23602 30839-30843 JJ denotes open
T23601 30836-30838 IN denotes as
T22839 12334-12335 . denotes .
T22894 12504-12508 JJ denotes wild
T22893 12501-12503 NN denotes bp
T22892 12497-12500 CD denotes 429
T22891 12514-12520 NN denotes signal
T22890 12493-12496 DT denotes the
T22889 12490-12492 IN denotes of
T22888 12482-12489 NN denotes absence
T22887 12478-12481 DT denotes the
T22886 12475-12477 IN denotes by
T22885 12468-12474 VBN denotes marked
T22884 12466-12468 , denotes ,
T22883 12465-12466 SYM denotes -
T22882 12464-12465 HYPH denotes /
T22881 12463-12464 SYM denotes -
T22880 12458-12463 NN denotes Capn2
T22879 12452-12453 CD denotes 4
T22878 12451-12452 SYM denotes #
T22877 12444-12450 NN denotes embryo
T22876 12454-12457 VBD denotes was
T22875 12438-12443 IN denotes while
T22874 12436-12437 SYM denotes -
T22873 12435-12436 HYPH denotes /
T22872 12434-12435 SYM denotes +
T22871 12429-12434 NN denotes Capn2
T22870 12422-12423 CD denotes 6
T22869 12418-12421 CC denotes and
T22868 12416-12418 , denotes ,
T22867 12415-12416 CD denotes 5
T22866 12413-12415 , denotes ,
T22865 12412-12413 CD denotes 3
T22864 12410-12412 , denotes ,
T22863 12409-12410 CD denotes 2
T22862 12407-12409 , denotes ,
T22861 12424-12428 VBD denotes were
T22860 12405-12406 SYM denotes #
T22859 12406-12407 CD denotes 1
T22858 12397-12404 NNS denotes Embryos
T22857 12396-12521 sentence denotes Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal.
T22856 12395-12396 . denotes .
T22855 12387-12389 VBZ denotes is
T22854 12379-12386 NNS denotes embryos
T22853 12373-12374 HYPH denotes -
T22852 12374-12378 NN denotes cell
T22851 12372-12373 CD denotes 8
T22850 12369-12371 IN denotes of
T22849 12358-12368 NN denotes genotyping
T22848 12354-12357 DT denotes the
T22847 12351-12353 IN denotes of
T22846 12343-12350 NN denotes example
T22845 12340-12342 DT denotes An
T22844 12338-12339 -RRB- denotes )
T22843 12390-12395 VBN denotes shown
T22842 12337-12338 LS denotes B
T22841 12336-12337 -LRB- denotes (
T22840 12335-12396 sentence denotes (B) An example of the genotyping of 8-cell embryos is shown.
T22838 12321-12327 NN denotes mutant
T22837 12318-12320 NN denotes bp
T22836 12314-12317 CD denotes 389
T22835 12328-12334 NN denotes signal
T22834 12310-12313 DT denotes the
T22833 12307-12309 IN denotes of
T22832 12299-12306 NN denotes absence
T22831 12295-12298 DT denotes the
T22830 12292-12294 IN denotes by
T22829 12284-12291 VBN denotes denoted
T22828 12282-12284 , denotes ,
T22827 12281-12282 SYM denotes +
T22826 12280-12281 HYPH denotes /
T22825 12279-12280 SYM denotes +
T22824 12274-12279 NN denotes Capn2
T22823 12267-12268 CD denotes 7
T22822 12266-12267 SYM denotes #
T22821 12262-12265 CC denotes and
T22820 12259-12260 SYM denotes #
T22819 12260-12261 CD denotes 3
T22818 12251-12258 NNS denotes embryos
T22817 12269-12273 VBD denotes were
T22816 12243-12250 IN denotes whereas
T22815 12241-12242 SYM denotes -
T22814 12240-12241 HYPH denotes /
T22813 12239-12240 SYM denotes +
T22812 12234-12239 NN denotes Capn2
T22811 12227-12228 CD denotes 6
T22810 12223-12226 CC denotes and
T22809 12221-12223 , denotes ,
T22808 12220-12221 CD denotes 5
T22807 12218-12220 , denotes ,
T22806 12217-12218 CD denotes 4
T22805 12215-12217 , denotes ,
T22804 12214-12215 CD denotes 2
T22803 12212-12214 , denotes ,
T22802 12229-12233 VBD denotes were
T22801 12210-12211 SYM denotes #
T22800 12211-12212 CD denotes 1
T22799 12202-12209 NNS denotes Embryos
T22798 12201-12335 sentence denotes Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal.
T22797 12200-12201 . denotes .
T22796 12187-12192 NN denotes stage
T22795 12193-12200 NNS denotes embryos
T22794 12176-12186 NN denotes blastocyst
T22793 12173-12175 IN denotes of
T22792 12162-12172 NN denotes genotyping
T22791 12158-12161 DT denotes the
T22790 12155-12157 IN denotes of
T22789 12132-12146 JJ denotes representative
T22788 12130-12131 DT denotes A
T22787 12128-12129 -RRB- denotes )
T22786 12147-12154 NN denotes example
T22785 12127-12128 LS denotes A
T22784 12126-12127 -LRB- denotes (
T22783 12125-12201 sentence denotes (A) A representative example of the genotyping of blastocyst stage embryos.
T22782 12124-12125 . denotes .
T22781 12108-12116 JJ denotes internal
T22780 12117-12124 NN denotes control
T22779 12104-12107 DT denotes the
T22778 12100-12103 IN denotes for
T22777 12097-12099 NN denotes bp
T22776 12093-12096 CD denotes 213
T22775 12089-12092 CC denotes and
T22774 12087-12089 , denotes ,
T22773 12074-12080 NN denotes mutant
T22772 12081-12087 NN denotes allele
T22771 12070-12073 DT denotes the
T22770 12066-12069 IN denotes for
T22769 12063-12065 NN denotes bp
T22768 12059-12062 CD denotes 389
T22767 12057-12059 , denotes ,
T22766 12045-12046 HYPH denotes -
T22765 12046-12050 NN denotes type
T22764 12041-12045 JJ denotes wild
T22763 12051-12057 NN denotes allele
T22762 12037-12040 DT denotes the
T22761 12033-12036 IN denotes for
T22760 12030-12032 NN denotes bp
T22759 12026-12029 CD denotes 429
T22758 12021-12025 VBD denotes were
T22757 12006-12011 JJ denotes final
T22756 12012-12020 NNS denotes products
T22755 12002-12005 DT denotes The
T22754 12001-12125 sentence denotes The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control.
T22753 12000-12001 . denotes .
T22752 11993-12000 NNS denotes alleles
T22751 11988-11992 DT denotes both
T22750 11985-11987 IN denotes in
T22749 11976-11978 VBZ denotes is
T22748 11979-11984 VBN denotes found
T22747 11970-11975 WDT denotes which
T22746 11968-11970 , denotes ,
T22745 11952-11961 NN denotes targeting
T22744 11962-11968 NN denotes vector
T22743 11948-11951 DT denotes the
T22742 11945-11947 IN denotes of
T22741 11939-11940 -RRB- denotes )
T22740 11931-11939 JJ denotes upstream
T22739 11930-11931 -LRB- denotes (
T22738 11924-11929 JJ denotes short
T22737 11941-11944 NN denotes arm
T22736 11920-11923 DT denotes the
T22735 11917-11919 IN denotes in
T22734 11909-11916 VBN denotes located
T22733 11907-11909 , denotes ,
T22732 11891-11898 NN denotes control
T22731 11882-11890 JJ denotes internal
T22730 11899-11907 NN denotes sequence
T22729 11879-11881 DT denotes an
T22728 11874-11878 IN denotes with
T22727 11856-11860 VBD denotes were
T22726 11848-11855 NNS denotes alleles
T22725 11841-11847 NN denotes mutant
T22724 11838-11840 CC denotes or
T22723 11832-11833 HYPH denotes -
T22722 11828-11832 JJ denotes wild
T22721 11824-11827 DT denotes the
T22720 11833-11837 NN denotes type
T22719 11817-11823 CC denotes either
T22718 11814-11816 IN denotes in
T23585 30765-30768 CC denotes and
T23584 30752-30753 HYPH denotes -
T23583 30753-30757 NN denotes type
T23582 30748-30752 JJ denotes wild
T23581 30758-30764 NN denotes allele
T23580 30744-30747 DT denotes the
T23579 30739-30743 IN denotes from
T23578 30724-30729 NN denotes BamHI
T23577 30720-30721 HYPH denotes -
T23576 30721-30723 NN denotes kb
T23575 30717-30720 CD denotes 3.5
T23574 30730-30738 NN denotes fragment
T23573 30715-30716 DT denotes a
T23572 30697-30702 JJ denotes short
T23571 30703-30706 NN denotes arm
T23570 30693-30696 DT denotes the
T23569 30690-30692 IN denotes of
T23568 30682-30689 JJ denotes outside
T23567 30670-30681 RB denotes immediately
T23566 30662-30669 VBN denotes located
T23565 30707-30714 VBZ denotes detects
T23564 30656-30661 NN denotes probe
T23563 30654-30655 DT denotes A
T23562 30653-30816 sentence denotes A probe located immediately outside of the short arm detects a 3.5-kb BamHI fragment from the wild-type allele and a 5.3-kb BamHI fragment from the mutant allele.
T23561 30652-30653 . denotes .
T23560 30647-30648 -RRB- denotes )
T23559 30643-30647 JJ denotes long
T23558 30642-30643 -LRB- denotes (
T23557 30631-30641 JJ denotes downstream
T23556 30649-30652 NN denotes arm
T23555 30627-30630 DT denotes the
T23554 30624-30626 IN denotes in
T23553 30615-30623 NN denotes homology
T23552 30612-30614 IN denotes of
T23551 30608-30609 HYPH denotes -
T23550 30609-30611 NN denotes kb
T23549 30605-30608 CD denotes 7.9
T23548 30601-30604 CC denotes and
T23547 30595-30596 -RRB- denotes )
T23546 30590-30595 JJ denotes short
T23545 30589-30590 -LRB- denotes (
T23544 30580-30588 JJ denotes upstream
T23543 30597-30600 NN denotes arm
T23542 30576-30579 DT denotes the
T23541 30573-30575 IN denotes in
T23540 30553-30563 JJ denotes homologous
T23539 30564-30572 NN denotes sequence
T23538 30547-30552 NN denotes Capn2
T23537 30544-30546 IN denotes of
T23536 30540-30541 HYPH denotes -
T23535 30541-30543 NN denotes kb
T23534 30537-30540 CD denotes 2.7
T23533 30534-30536 IN denotes by
T23532 30523-30525 VBZ denotes is
T23531 30509-30510 HYPH denotes -
T23530 30510-30513 NN denotes Neo
T23529 30506-30509 NN denotes PGK
T23528 30514-30522 NN denotes cassette
T23527 30502-30505 DT denotes the
T23526 30500-30502 , denotes ,
T23525 30484-30493 NN denotes targeting
T23524 30494-30500 NN denotes vector
T23523 30480-30483 DT denotes the
T23522 30477-30479 IN denotes In
T23521 30475-30476 -RRB- denotes )
T23520 30469-30475 NN denotes Asn286
T23519 30468-30469 -LRB- denotes (
T23518 30449-30459 NN denotes asparagine
T23517 30444-30448 NN denotes site
T23516 30437-30443 JJ denotes active
T23515 30460-30467 NN denotes residue
T23514 30433-30436 DT denotes the
T23513 30425-30432 VBZ denotes encodes
T23512 30419-30424 WDT denotes which
T23511 30417-30418 -RRB- denotes )
T23510 30403-30407 JJ denotes grey
T23509 30408-30417 NN denotes rectangle
T23508 30402-30403 -LRB- denotes (
T23507 30400-30401 CD denotes 7
T23506 30395-30399 NN denotes exon
T23505 30384-30394 VBG denotes containing
T23504 30367-30374 JJ denotes genomic
T23503 30363-30364 HYPH denotes -
T23502 30364-30366 NN denotes kb
T23501 30360-30363 CD denotes 0.8
T23500 30375-30383 NN denotes fragment
T23499 30358-30359 DT denotes a
T23498 30526-30533 VBN denotes flanked
T23497 30335-30336 HYPH denotes -
T23496 30336-30339 NN denotes Neo
T23495 30332-30335 NN denotes PGK
T23494 30340-30348 NN denotes cassette
T23493 30330-30331 DT denotes a
T23492 30328-30330 , denotes ,
T23491 30315-30321 NN denotes mutant
T23490 30322-30328 NN denotes allele
T23489 30311-30314 DT denotes the
T23488 30349-30357 VBZ denotes replaces
T23487 30308-30310 IN denotes In
T23486 30307-30653 sentence denotes In the mutant allele, a PGK-Neo cassette replaces a 0.8-kb genomic fragment containing exon 7 (grey rectangle) which encodes the active site asparagine residue (Asn286) In the targeting vector, the PGK-Neo cassette is flanked by 2.7-kb of Capn2 homologous sequence in the upstream (short) arm and 7.9-kb of homology in the downstream (long) arm.
T23485 30306-30307 . denotes .
T23484 30294-30297 VBP denotes are
T23483 30292-30293 -RRB- denotes )
T23482 30286-30292 JJ denotes bottom
T23481 30285-30286 -LRB- denotes (
T23480 30271-30277 NN denotes mutant
T23479 30278-30284 NN denotes allele
T23478 30267-30270 DT denotes the
T23477 30263-30266 CC denotes and
T23476 30261-30263 , denotes ,
T23475 30260-30261 -RRB- denotes )
T23474 30254-30260 JJ denotes middle
T23473 30253-30254 -LRB- denotes (
T23472 30236-30245 NN denotes targeting
T23471 30246-30252 NN denotes vector
T23470 30232-30235 DT denotes the
T23469 30230-30232 , denotes ,
T23468 30229-30230 -RRB- denotes )
T23467 30226-30229 JJ denotes top
T23466 30225-30226 -LRB- denotes (
T23465 30212-30213 HYPH denotes -
T23464 30213-30217 NN denotes type
T23463 30208-30212 JJ denotes wild
T23462 30218-30224 NN denotes allele
T23461 30204-30207 DT denotes the
T23460 30201-30203 IN denotes of
T23459 30298-30306 VBN denotes depicted
T23458 30190-30200 NNS denotes structures
T23457 30186-30189 DT denotes The
T23456 30185-30307 sentence denotes The structures of the wild-type allele (top), the targeting vector (middle), and the mutant allele (bottom) are depicted.
T23455 30184-30185 . denotes .
T23454 30171-30184 NN denotes recombination
T23453 30160-30170 JJ denotes homologous
T23452 30157-30159 IN denotes by
T23451 30151-30156 NNS denotes cells
T23450 30148-30150 NN denotes ES
T23449 30145-30147 IN denotes in
T23448 30131-30134 VBD denotes was
T23447 30129-30131 , denotes ,
T23446 30117-30119 CD denotes 80
T23445 30116-30117 HYPH denotes -
T23444 30120-30121 NN denotes k
T23443 30115-30116 NN denotes m
T23442 30122-30129 NN denotes subunit
T23441 30111-30114 DT denotes the
T23440 30102-30110 VBG denotes encoding
T23439 30100-30102 , denotes ,
T23438 30135-30144 VBN denotes disrupted
T23437 30090-30095 NN denotes Capn2
T23436 30083-30089 JJ denotes murine
T23435 30096-30100 NN denotes gene
T23434 30079-30082 DT denotes The
T23433 30078-30185 sentence denotes The murine Capn2 gene, encoding the m-80 k subunit, was disrupted in ES cells by homologous recombination.
T23432 30077-30078 . denotes .
T23431 30067-30072 NN denotes Capn2
T23430 30060-30066 JJ denotes murine
T23429 30073-30077 NN denotes gene
T23428 30056-30059 DT denotes the
T23427 30053-30055 IN denotes of
T23426 30042-30052 NN denotes disruption
T23425 30038-30041 IN denotes for
T23424 30029-30037 NN denotes strategy
T23423 30019-30028 NN denotes Targeting
T22717 11802-11813 RB denotes exclusively
T22716 11796-11801 VBN denotes found
T22715 11777-11786 NNS denotes reactions
T22714 11787-11795 NNS denotes segments
T22713 11768-11776 JJ denotes separate
T22712 11861-11873 VBN denotes co-amplified
T22711 11765-11767 IN denotes In
T22710 11764-12001 sentence denotes In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles.
T22709 11763-11764 . denotes .
T22708 11762-11763 NN denotes K
T22707 11751-11761 NN denotes proteinase
T22706 11746-11750 IN denotes with
T22705 11737-11745 VBN denotes digested
T22704 11732-11736 RB denotes then
T22703 11728-11731 CC denotes and
T22702 11719-11727 NNS denotes oviducts
T22701 11715-11718 DT denotes the
T22700 11710-11714 IN denotes from
T22699 11697-11701 VBD denotes were
T22698 11695-11696 -RRB- denotes )
T22697 11691-11695 NN denotes E2.5
T22696 11690-11691 -LRB- denotes (
T22695 11702-11709 VBN denotes flushed
T22694 11676-11677 HYPH denotes -
T22693 11677-11681 NN denotes cell
T22692 11675-11676 CD denotes 8
T22691 11672-11674 CC denotes or
T22690 11670-11671 -RRB- denotes )
T22689 11666-11670 NN denotes E3.5
T22688 11665-11666 -LRB- denotes (
T22687 11682-11689 NNS denotes embryos
T22686 11654-11664 NN denotes Blastocyst
T22685 11653-11764 sentence denotes Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K.
T22684 11652-11653 . denotes .
T22683 11647-11652 NNS denotes plugs
T22665 11551-11556 NN denotes Capn2
T22664 11550-11653 sentence denotes Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs.
T22663 11549-11550 . denotes .
T22662 11548-11549 CD denotes 4
T22661 11541-11547 NN denotes Figure
T22660 11538-11540 IN denotes in
T22659 11528-11537 VBN denotes described
T22658 11525-11527 IN denotes as
T22657 11512-11524 NN denotes implantation
T22656 11509-11511 IN denotes to
T22655 11503-11508 JJ denotes prior
T22654 11495-11502 NNS denotes embryos
T22653 11486-11494 VB denotes genotype
T22652 11483-11485 TO denotes to
T22651 11474-11477 VBD denotes was
T22650 11478-11482 VBN denotes used
T22649 11461-11464 NN denotes PCR
T22648 11454-11460 VBN denotes nested
T22647 11465-11473 NN denotes strategy
T22646 11452-11453 DT denotes A
T22645 11451-11550 sentence denotes A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4.
T22644 11450-11451 . denotes .
T22643 11443-11450 NNS denotes embryos
T22642 11426-11442 JJ denotes pre-implantation
T22641 11423-11425 IN denotes of
T22640 11412-11422 NN denotes Genotyping
T23600 30823-30826 VBP denotes are
T23599 30827-30835 VBN denotes depicted
T23598 30817-30822 NNS denotes Exons
T23597 30816-30933 sentence denotes Exons are depicted as open vertical rectangles except for exon 7 which is represented by a solid vertical rectangle.
T23596 30815-30816 . denotes .
T23595 30802-30808 NN denotes mutant
T23594 30809-30815 NN denotes allele
T23593 30798-30801 DT denotes the
T23592 30793-30797 IN denotes from
T23591 30778-30783 NN denotes BamHI
T23590 30774-30775 HYPH denotes -
T23589 30775-30777 NN denotes kb
T23588 30771-30774 CD denotes 5.3
T23587 30784-30792 NN denotes fragment
T23586 30769-30770 DT denotes a
T22907 12561-12562 . denotes .
T22906 12548-12554 NN denotes weight
T22905 12538-12547 JJ denotes molecular
T22904 12555-12561 NN denotes marker
T22903 12534-12537 DT denotes the
T22902 12524-12525 -RRB- denotes )
T22901 12526-12533 VBZ denotes denotes
T22900 12523-12524 NN denotes M
T22899 12522-12523 -LRB- denotes (
T22682 11639-11646 JJ denotes vaginal
T22681 11636-11638 IN denotes of
T22680 11625-11635 NN denotes appearance
T22679 11621-11624 DT denotes the
T22678 11618-11620 IN denotes by
T22677 11592-11605 NN denotes fertilization
T22676 11589-11591 IN denotes of
T22675 11606-11617 VBN denotes established
T22674 11584-11588 NN denotes date
T22673 11580-11583 DT denotes the
T22672 11576-11579 CC denotes and
T22671 11565-11569 VBD denotes were
T22670 11570-11575 VBN denotes mated
T22669 11558-11559 SYM denotes -
T22668 11557-11558 HYPH denotes /
T22667 11556-11557 SYM denotes +
T22666 11560-11564 NNS denotes mice
T22084 11400-11401 . denotes .
T22083 11387-11393 NN denotes mutant
T22082 11394-11400 NN denotes allele
T22081 11383-11386 DT denotes the
T22080 11380-11382 IN denotes in
T22079 11366-11367 HYPH denotes -
T22078 11367-11370 NN denotes Neo
T22077 11363-11366 NN denotes PGK
T22076 11371-11379 NN denotes cassette
T22075 11359-11362 DT denotes the
T22074 11356-11358 IN denotes by
T22073 11344-11346 VBZ denotes is
T22072 11347-11355 VBN denotes replaced
T22071 11339-11343 WDT denotes that
T22070 11326-11327 HYPH denotes -
T22069 11327-11331 NN denotes type
T22068 11322-11326 JJ denotes wild
T22067 11332-11338 NN denotes allele
T22066 11318-11321 DT denotes the
T22065 11315-11317 IN denotes of
T22064 11307-11314 NN denotes segment
T22063 11303-11306 DT denotes the
T22062 11292-11302 VBZ denotes delineates
T22061 11271-11281 JJ denotes horizontal
T22060 11269-11271 , denotes ,
T22059 11265-11269 JJ denotes grey
T22058 11282-11291 NN denotes rectangle
T22057 11263-11264 DT denotes A
T22056 11262-11401 sentence denotes A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele.
T22055 11261-11262 . denotes .
T22054 11256-11257 -RRB- denotes )
T22053 11246-11256 JJ denotes downstream
T22052 11245-11246 -LRB- denotes (
T22051 11240-11244 JJ denotes long
T22050 11258-11261 NN denotes arm
T22049 11236-11239 DT denotes the
T22048 11233-11235 IN denotes of
T22047 11227-11228 SYM denotes '
T22046 11226-11227 CD denotes 5
T22045 11229-11232 NN denotes end
T22044 11222-11225 DT denotes the
T22043 11218-11221 CC denotes and
T22042 11208-11213 JJ denotes short
T22041 11214-11217 NN denotes arm
T22040 11204-11207 DT denotes the
T22039 11201-11203 IN denotes of
T22038 11190-11200 NNS denotes boundaries
T22037 11186-11189 DT denotes the
T22036 11164-11172 JJ denotes vertical
T22035 11173-11178 NNS denotes lines
T22034 11159-11163 JJ denotes thin
T22033 11179-11185 VBP denotes denote
T22032 11153-11158 IN denotes while
T22031 11151-11153 , denotes ,
T22030 11131-11141 JJ denotes horizontal
T22029 11129-11131 , denotes ,
T22028 11125-11129 JJ denotes open
T22027 11142-11151 NN denotes rectangle
T22026 11122-11124 DT denotes an
T22025 11119-11121 IN denotes by
T22024 11105-11106 HYPH denotes -
T22023 11106-11109 NN denotes Neo
T22022 11102-11105 NN denotes PGK
T22021 11098-11101 DT denotes the
T22020 11096-11098 , denotes ,
T22019 11077-11085 JJ denotes vertical
T22018 11075-11077 , denotes ,
T22017 11086-11096 NNS denotes rectangles
T22016 11071-11075 JJ denotes open
T22015 11068-11070 IN denotes by
T22014 11110-11118 NN denotes cassette
T22013 11052-11055 VBP denotes are
T22012 11056-11067 VBN denotes represented
T22011 11046-11051 NNS denotes Exons
T22010 11045-11262 sentence denotes Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm.
T22009 11044-11045 . denotes .
T22008 11029-11034 CD denotes three
T22007 11035-11044 NNS denotes reactions
T22006 11025-11028 DT denotes the
T22005 11022-11024 IN denotes of
T22004 11017-11021 DT denotes each
T22003 11013-11016 IN denotes for
T22002 11006-11007 -RRB- denotes )
T22001 11000-11006 VBN denotes nested
T22000 10999-11000 -LRB- denotes (
T21999 11008-11012 NNS denotes sets
T21998 10995-10998 CD denotes two
T21997 10992-10994 IN denotes in
T21996 10979-10982 VBP denotes are
T21995 10977-10979 , denotes ,
T21994 10968-10977 NNS denotes triangles
T21993 10965-10967 IN denotes by
T21992 10953-10964 VBN denotes represented
T21991 10951-10953 , denotes ,
T21990 10983-10991 VBN denotes depicted
T21989 10944-10951 NNS denotes primers
T21988 10940-10943 DT denotes The
T21987 10939-11045 sentence denotes The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions.
T21986 10938-10939 . denotes .
T21985 10929-10930 HYPH denotes -
T21984 10930-10938 JJ denotes specific
T21983 10923-10929 NN denotes allele
T21982 10900-10909 NN denotes antisense
T21981 10910-10917 NNS denotes primers
T21980 10896-10899 DT denotes the
T21979 10918-10922 VBD denotes were
T21978 10888-10895 IN denotes whereas
T21977 10886-10888 , denotes ,
T21976 10877-10886 NNS denotes reactions
T21975 10872-10876 DT denotes both
T21974 10869-10871 IN denotes in
T21973 10857-10858 '' denotes '
T21972 10859-10868 NNS denotes sequences
T21971 10847-10857 JJ denotes diagnostic
T21970 10846-10847 `` denotes '
T21969 10838-10845 VB denotes amplify
T21968 10835-10837 TO denotes to
T21967 10825-10829 VBD denotes were
T21966 10830-10834 VBN denotes used
T21965 10811-10816 NN denotes sense
T21964 10806-10810 JJ denotes same
T21963 10817-10824 NNS denotes primers
T21962 10802-10805 DT denotes The
T21961 10801-10939 sentence denotes The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific.
T21960 10800-10801 . denotes .
T21959 10784-10792 JJ denotes internal
T21958 10793-10800 NN denotes control
T21957 10781-10783 DT denotes an
T21956 10778-10780 IN denotes as
T21955 10767-10768 '' denotes '
T21954 10757-10767 JJ denotes diagnostic
T21953 10756-10757 `` denotes '
T21952 10769-10777 NNS denotes products
T21951 10752-10755 DT denotes the
T21950 10747-10751 IN denotes with
T21949 10730-10733 VBD denotes was
T21948 10720-10725 JJ denotes short
T21947 10726-10729 NN denotes arm
T21946 10716-10719 DT denotes the
T21945 10709-10715 IN denotes within
T21944 10701-10708 VBN denotes located
T21943 10689-10691 NN denotes bp
T21942 10685-10688 CD denotes 213
T21941 10692-10700 NN denotes sequence
T21940 10683-10684 DT denotes a
T21939 10681-10683 , denotes ,
T21938 10672-10681 NNS denotes reactions
T21937 10667-10671 DT denotes both
T21936 10734-10746 VBN denotes co-amplified
T21935 10664-10666 IN denotes In
T21934 10663-10801 sentence denotes In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control.
T21933 10662-10663 . denotes .
T21932 10646-10655 NN denotes targeting
T21931 10656-10662 NN denotes vector
T21930 10642-10645 DT denotes the
T21929 10639-10641 IN denotes of
T21928 10633-10634 -RRB- denotes )
T21927 10625-10633 JJ denotes upstream
T21926 10624-10625 -LRB- denotes (
T21925 10618-10623 JJ denotes short
T21924 10635-10638 NN denotes arm
T21923 10614-10617 DT denotes the
T21922 10611-10613 IN denotes of
T21921 10605-10606 SYM denotes '
T21920 10604-10605 CD denotes 3
T21919 10607-10610 NN denotes end
T21918 10600-10603 DT denotes the
T21917 10591-10599 VBG denotes spanning
T21916 10586-10590 DT denotes both
T21915 10584-10586 , denotes ,
T21914 10571-10577 NN denotes mutant
T21913 10578-10584 NN denotes allele
T21912 10567-10570 DT denotes the
T21911 10562-10566 IN denotes from
T21910 10551-10553 NN denotes bp
T21909 10547-10550 CD denotes 389
T21908 10554-10561 NN denotes segment
T21907 10545-10546 DT denotes a
T21906 10541-10544 CC denotes and
T21905 10528-10529 HYPH denotes -
T21904 10529-10533 NN denotes type
T21903 10524-10528 JJ denotes wild
T21902 10534-10540 NN denotes allele
T21901 10520-10523 DT denotes the
T21900 10515-10519 IN denotes from
T21899 10503-10505 NN denotes bp
T21898 10499-10502 CD denotes 429
T21897 10506-10514 NN denotes fragment
T21896 10497-10498 DT denotes a
T21895 10489-10496 VB denotes amplify
T21894 10486-10488 TO denotes to
T21893 10476-10480 VBD denotes were
T21892 10481-10485 VBN denotes used
T21891 10466-10475 NNS denotes reactions
T21890 10457-10465 JJ denotes Separate
T21889 10456-10663 sentence denotes Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector.
T21888 10455-10456 . denotes .
T21887 10448-10455 NNS denotes embryos
T21886 10431-10447 JJ denotes pre-implantation
T21885 10422-10430 VB denotes genotype
T21884 10419-10421 TO denotes to
T21883 10413-10418 NN denotes order
T21882 10410-10412 IN denotes in
T21881 10396-10399 VBD denotes was
T21880 10383-10386 NN denotes PCR
T21879 10376-10382 VBN denotes nested
T21878 10387-10395 NN denotes strategy
T21877 10374-10375 DT denotes a
T21876 10372-10374 , denotes ,
T21875 10356-10363 JJ denotes genetic
T21874 10364-10372 NN denotes material
T21873 10344-10355 JJ denotes extractable
T21872 10341-10343 IN denotes of
T21871 10332-10340 NN denotes scarcity
T21870 10328-10331 DT denotes the
T21869 10325-10327 IN denotes to
T21868 10400-10409 VBN denotes developed
T21867 10321-10324 IN denotes Due
T21866 10320-10456 sentence denotes Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos.
T21865 10319-10320 . denotes .
T21864 10312-10319 NNS denotes embryos
T21863 10295-10311 JJ denotes pre-implantation
T21862 10292-10294 IN denotes of
T21861 10281-10291 NN denotes genotyping
T21860 10277-10280 IN denotes for
T21859 10264-10267 NN denotes PCR
T21858 10268-10276 NN denotes strategy
T21857 10257-10263 VBN denotes Nested
T20576 37872-37874 , denotes ,
T20575 37869-37872 CD denotes 100
T20574 37868-37869 HYPH denotes -
T20573 37860-37866 NNP denotes Triton
T20572 37867-37868 NN denotes X
T20571 37858-37859 NN denotes %
T20570 37855-37858 CD denotes 0.5
T20569 37853-37855 , denotes ,
T20568 37849-37850 HYPH denotes -
T20567 37845-37849 NN denotes Tris
T20566 37850-37853 NN denotes HCl
T20565 37842-37844 NN denotes mM
T20564 37839-37841 CD denotes 50
T20563 37898-37900 NN denotes pH
T20562 37838-37839 -LRB- denotes (
T20561 37831-37837 NN denotes buffer
T20560 37825-37830 NN denotes lysis
T20559 37822-37824 IN denotes of
T20558 37819-37821 NN denotes μL
T20557 37816-37818 CD denotes 20
T20556 37813-37815 IN denotes in
T20555 37810-37812 NN denotes °C
T20554 37808-37810 CD denotes 55
T20553 37805-37807 IN denotes at
T20552 37799-37804 NNS denotes hours
T20551 37794-37798 CD denotes five
T20550 37790-37793 IN denotes for
T20549 37776-37780 RB denotes then
T20548 37771-37775 VBD denotes were
T20547 37781-37789 VBN denotes digested
T20546 37763-37770 NNS denotes embryos
T20545 37754-37762 VBN denotes Isolated
T20544 37753-37970 sentence denotes Isolated embryos were then digested for five hours at 55°C in 20 μL of lysis buffer (50 mM Tris-HCl, 0.5% Triton X-100, 200 μL/mL proteinase K, pH 8.0), followed by ten minutes at 95°C to inactivate the proteinase K.
T20543 37752-37753 . denotes .
T20542 37741-37745 NN denotes cell
T20541 37746-37752 NN denotes medium
T20540 37738-37740 NN denotes ES
T20539 37733-37737 IN denotes with
T20538 37724-37732 NNS denotes oviducts
T20537 37720-37723 DT denotes the
T20536 37711-37719 VBG denotes flushing
T20535 37707-37710 CC denotes and
T20534 37702-37706 NN denotes E3.5
T20533 37699-37701 CC denotes or
T20532 37694-37698 NN denotes E2.5
T20531 37691-37693 IN denotes at
T20530 37683-37690 NNS denotes females
T20529 37674-37682 JJ denotes pregnant
T20528 37671-37673 IN denotes of
T20527 37667-37670 IN denotes out
T20526 37661-37666 NNS denotes uteri
T20525 37657-37660 DT denotes the
T20524 37646-37656 VBG denotes dissecting
T20523 37643-37645 IN denotes by
T20522 37629-37633 VBD denotes were
T20521 37634-37642 VBN denotes obtained
T20520 37621-37628 NNS denotes embryos
T20519 37604-37620 JJ denotes Pre-implantation
T20518 37603-37753 sentence denotes Pre-implantation embryos were obtained by dissecting the uteri out of pregnant females at E2.5 or E3.5 and flushing the oviducts with ES cell medium.
T20517 37602-37603 . denotes .
T20516 37598-37602 NN denotes E0.5
T20232 37448-37449 . denotes .
T20231 37441-37448 NNS denotes minutes
T20230 37437-37440 CD denotes ten
T20229 37434-37436 IN denotes of
T20228 37419-37428 NN denotes extension
T20227 37413-37418 JJ denotes final
T20226 37429-37433 NN denotes step
T20225 37411-37412 DT denotes a
T20224 37406-37410 IN denotes with
T20223 37404-37406 , denotes ,
T20222 37402-37404 NN denotes °C
T20221 37400-37402 CD denotes 72
T20220 37397-37399 IN denotes at
T20219 37387-37396 NN denotes extension
T20218 37380-37386 NN denotes minute
T20217 37376-37379 CD denotes one
T20216 37372-37375 CC denotes and
T20215 37370-37372 , denotes ,
T20214 37368-37370 NN denotes °C
T20213 37366-37368 CD denotes 56
T20212 37363-37365 IN denotes at
T20211 37353-37362 VBG denotes annealing
T20210 37346-37352 NN denotes minute
T20209 37342-37345 CD denotes one
T20208 37340-37342 , denotes ,
T20066 36639-36641 IN denotes In
T20065 36638-36854 sentence denotes In addition, the two sets of reactions included a common internal control designed to amplify a genomic region within the short arm of the targeting vector that is preserved in both the wild-type and mutant alleles.
T20064 36637-36638 . denotes .
T20063 36625-36628 NN denotes PCR
T20062 36618-36624 VBN denotes nested
T20061 36629-36637 NN denotes strategy
T20060 36614-36617 DT denotes the
T20059 36611-36613 IN denotes of
T20058 36605-36610 NNS denotes steps
T20057 36600-36604 DT denotes both
T20056 36597-36599 IN denotes in
T20055 36583-36588 NN denotes sense
T20054 36578-36582 JJ denotes same
T20053 36589-36596 NNS denotes primers
T20052 36574-36577 DT denotes the
T20051 36555-36564 NNS denotes sequences
T20050 36550-36554 DT denotes both
T20049 36547-36549 IN denotes of
T20048 36533-36546 NN denotes amplification
T20047 36565-36573 VBD denotes involved
T20046 36528-36532 IN denotes that
T20045 36519-36521 VB denotes be
T20044 36512-36518 MD denotes should
T20043 36522-36527 VBN denotes noted
T20042 36509-36511 PRP denotes It
T20041 36508-36638 sentence denotes It should be noted that amplification of both sequences involved the same sense primers in both steps of the nested PCR strategy.
T20040 36507-36508 . denotes .
T20039 36494-36495 HYPH denotes -
T20038 36495-36498 NN denotes Neo
T20037 36491-36494 NN denotes PGK
T20036 36499-36507 NN denotes sequence
T20035 36487-36490 DT denotes the
T20034 36480-36486 IN denotes within
T20033 36472-36479 VBN denotes located
T20032 36467-36471 RB denotes also
T20031 36450-36459 NN denotes antisense
T20030 36460-36466 NN denotes primer
T20029 36447-36449 DT denotes an
T20028 36443-36446 CC denotes and
T20027 36430-36435 NN denotes sense
T20026 36428-36429 CD denotes 6
T20025 36421-36427 NN denotes intron
T20024 36414-36420 JJ denotes second
T20023 36436-36442 NN denotes primer
T20022 36410-36413 DT denotes the
T20021 36407-36409 IN denotes of
T20020 36393-36396 VBD denotes was
T20019 36397-36406 VBN denotes comprised
T20018 36381-36387 NN denotes primer
T20017 36374-36380 VBN denotes nested
T20016 36388-36392 NN denotes pair
T20015 36370-36373 DT denotes The
T20014 36369-36508 sentence denotes The nested primer pair was comprised of the second intron 6 sense primer and an antisense primer also located within the PGK-Neo sequence.
T20013 36368-36369 . denotes .
T20012 36355-36356 HYPH denotes -
T20011 36356-36359 NN denotes Neo
T20010 36352-36355 NN denotes PGK
T20009 36360-36368 NN denotes cassette
T20008 36348-36351 DT denotes the
T20007 36345-36347 IN denotes in
T20006 36328-36337 NN denotes antisense
T20005 36338-36344 NN denotes primer
T20004 36325-36327 DT denotes an
T20003 36321-36324 CC denotes and
T20002 36308-36313 NN denotes sense
T20001 36306-36307 CD denotes 6
T20000 36299-36305 NN denotes intron
T19999 36314-36320 NN denotes primer
T19998 36295-36298 DT denotes the
T19997 36292-36294 IN denotes of
T19996 36282-36291 VBD denotes consisted
T19995 36270-36276 NN denotes primer
T19994 36264-36269 JJ denotes first
T19993 36277-36281 NN denotes pair
T19992 36260-36263 DT denotes The
T19991 36259-36369 sentence denotes The first primer pair consisted of the intron 6 sense primer and an antisense primer in the PGK-Neo cassette.
T19990 36258-36259 . denotes .
T19989 36249-36258 RB denotes similarly
T19988 36245-36248 RP denotes out
T19987 36233-36236 VBD denotes was
T19986 36219-36225 NN denotes mutant
T19985 36226-36232 NN denotes allele
T19984 36215-36218 DT denotes the
T19983 36212-36214 IN denotes of
T19982 36237-36244 VBN denotes carried
T19981 36191-36197 VBN denotes nested
T19980 36198-36211 NN denotes amplification
T19979 36187-36190 DT denotes The
T19978 36186-36259 sentence denotes The nested amplification of the mutant allele was carried out similarly.
T19977 36185-36186 . denotes .
T19976 36184-36185 CD denotes 7
T19975 36179-36183 NN denotes exon
T19974 36176-36178 IN denotes in
T19973 36159-36168 NN denotes antisense
T19972 36169-36175 NN denotes primer
T19971 36156-36158 DT denotes an
T19970 36152-36155 CC denotes and
T19969 36139-36144 NN denotes sense
T19968 36137-36138 CD denotes 6
T19967 36130-36136 NN denotes intron
T19966 36145-36151 NN denotes primer
T19965 36122-36129 DT denotes another
T19964 36116-36121 VBG denotes using
T19963 36098-36101 NN denotes PCR
T19962 36091-36097 JJ denotes second
T19961 36102-36115 NN denotes amplification
T19960 36087-36090 DT denotes the
T19959 36084-36086 IN denotes in
T19958 36075-36083 NN denotes template
T19957 36072-36074 IN denotes as
T19956 36062-36066 VBD denotes were
T19955 36047-36052 JJ denotes first
T19954 36053-36061 NN denotes reaction
T19953 36043-36046 DT denotes the
T19952 36040-36042 IN denotes of
T19951 36067-36071 VBN denotes used
T19950 36037-36039 NN denotes μL
T19949 36033-36036 CD denotes Two
T19948 36032-36186 sentence denotes Two μL of the first reaction were used as template in the second PCR amplification using another intron 6 sense primer and an antisense primer in exon 7.
T19947 36031-36032 . denotes .
T19946 36030-36031 CD denotes 7
T19945 36023-36029 NN denotes intron
T19944 36020-36022 IN denotes in
T19943 36012-36019 VBN denotes located
T19942 35995-36004 NN denotes antisense
T19941 36005-36011 NN denotes primer
T19940 35992-35994 DT denotes an
T19939 35988-35991 CC denotes and
T19938 35975-35980 NN denotes sense
T19937 35973-35974 CD denotes 6
T19936 35966-35972 NN denotes intron
T19935 35981-35987 NN denotes primer
T19934 35963-35965 DT denotes an
T19933 35957-35962 VBG denotes using
T19932 35955-35957 , denotes ,
T19931 35953-35955 NN denotes μL
T19930 35950-35952 CD denotes 50
T19929 35947-35949 IN denotes of
T19928 35931-35939 NN denotes reaction
T19927 35925-35930 JJ denotes final
T19926 35940-35946 NN denotes volume
T19925 35923-35924 DT denotes a
T19924 35920-35922 IN denotes in
T19923 35916-35919 RP denotes out
T19922 35904-35907 VBD denotes was
T19921 35891-35892 HYPH denotes -
T19920 35892-35896 NN denotes type
T19919 35887-35891 JJ denotes wild
T19918 35897-35903 NN denotes allele
T19917 35883-35886 DT denotes the
T19916 35880-35882 IN denotes of
T19915 35862-35865 NN denotes PCR
T19914 35855-35861 VBN denotes nested
T19913 35866-35879 NN denotes amplification
T19912 35851-35854 DT denotes the
T19911 35848-35850 IN denotes in
T19910 35908-35915 VBN denotes carried
T19909 35833-35838 JJ denotes first
T19908 35839-35847 NN denotes reaction
T19907 35829-35832 DT denotes The
T19906 35828-36032 sentence denotes The first reaction in the nested PCR amplification of the wild-type allele was carried out in a final reaction volume of 50 μL, using an intron 6 sense primer and an antisense primer located in intron 7.
T19905 35827-35828 . denotes .
T19904 35814-35820 NN denotes mutant
T19903 35821-35827 NN denotes allele
T19902 35810-35813 DT denotes the
T19901 35807-35809 IN denotes of
T19900 35793-35806 NN denotes amplification
T19899 35789-35792 DT denotes the
T19898 35786-35788 IN denotes in
T19897 35780-35782 CD denotes 10
T19896 35770-35779 VBG denotes remaining
T19895 35783-35785 NN denotes μL
T19894 35766-35769 DT denotes the
T19893 35762-35765 CC denotes and
T19892 35749-35750 HYPH denotes -
T19891 35750-35754 NN denotes type
T19890 35745-35749 JJ denotes wild
T19889 35755-35761 NN denotes allele
T19888 35741-35744 DT denotes the
T19887 35738-35740 IN denotes of
T19886 35724-35737 NN denotes amplification
T20515 37595-37597 IN denotes as
T20514 37583-37586 VBD denotes was
T20513 37579-37582 NN denotes day
T20512 37574-37578 DT denotes that
T20511 37571-37573 IN denotes of
T20510 37587-37594 VBN denotes defined
T20509 37566-37570 NN denotes noon
T20508 37562-37565 CC denotes and
T20507 37560-37562 , denotes ,
T20506 37555-37560 NNS denotes plugs
T20505 37544-37554 NN denotes copulation
T20504 37541-37543 IN denotes of
T20503 37529-37540 NN denotes observation
T20502 37526-37528 IN denotes by
T20501 37511-37514 VBD denotes was
T20500 37497-37510 NN denotes fertilization
T20499 37494-37496 IN denotes of
T20498 37515-37525 VBN denotes determined
T20497 37489-37493 NN denotes Time
T20496 37488-37603 sentence denotes Time of fertilization was determined by observation of copulation plugs, and noon of that day was defined as E0.5.
T20495 37481-37488 NNS denotes embryos
T20494 37464-37480 JJ denotes pre-implantation
T20493 37461-37463 IN denotes of
T20492 37451-37460 NN denotes Isolation
T19885 35720-35723 DT denotes the
T19884 35717-35719 IN denotes in
T19883 35706-35711 VBG denotes being
T19882 35704-35705 -RRB- denotes )
T19881 35699-35701 CD denotes 10
T19880 35702-35704 NN denotes μL
T19879 35698-35699 -LRB- denotes (
T19878 35712-35716 VBN denotes used
T19877 35693-35697 NN denotes half
T19876 35688-35692 IN denotes with
T19875 35686-35688 , denotes ,
T19874 35683-35686 CD denotes two
T19873 35680-35682 IN denotes in
T19872 35667-35671 RB denotes then
T19871 35663-35666 VBD denotes was
T19870 35672-35679 VBN denotes divided
T19869 35656-35662 NN denotes lysate
T19868 35652-35655 DT denotes The
T19867 35651-35828 sentence denotes The lysate was then divided in two, with half (10 μL) being used in the amplification of the wild-type allele and the remaining 10 μL in the amplification of the mutant allele.
T19866 35650-35651 . denotes .
T19865 35649-35650 -RRB- denotes )
T19864 35644-35649 RB denotes below
T19863 35640-35643 VB denotes see
T19862 35639-35640 -LRB- denotes (
T19861 35632-35638 NN denotes buffer
T19860 35630-35631 NN denotes K
T19859 35619-35629 NN denotes proteinase
T19858 35616-35618 IN denotes of
T19857 35613-35615 NN denotes μL
T19856 35610-35612 CD denotes 20
T19855 35607-35609 IN denotes in
T19854 35592-35597 RB denotes first
T19853 35587-35591 VBD denotes were
T19852 35598-35606 VBN denotes digested
T19851 35579-35586 NNS denotes embryos
T19768 34168-34169 -RRB- denotes )
T19767 34161-34166 NN denotes Table
T19766 34167-34168 NN denotes I
T19765 34160-34161 -LRB- denotes (
T19764 34149-34150 -RRB- denotes )
T19763 34145-34146 HYPH denotes -
T19762 34142-34145 NN denotes PGK
T19761 34146-34149 NN denotes Neo
T19760 34141-34142 -LRB- denotes (
T19759 34134-34140 NN denotes mutant
T19758 34131-34133 CC denotes or
T19757 34129-34130 -RRB- denotes )
T19756 34128-34129 CD denotes 7
T19755 34123-34127 NN denotes exon
T19754 34122-34123 -LRB- denotes (
T19753 34151-34159 NN denotes sequence
T19752 34116-34117 HYPH denotes -
T19751 34112-34116 JJ denotes wild
T19750 34117-34121 NN denotes type
T19749 34105-34111 CC denotes either
T19748 34102-34104 IN denotes to
T19747 34091-34101 VBD denotes hybridized
T19746 34085-34090 WDT denotes which
T19745 34067-34076 NN denotes antisense
T19744 34077-34084 NNS denotes primers
T19743 34058-34066 JJ denotes distinct
T19742 34054-34057 CC denotes and
T19741 34052-34054 , denotes ,
T19740 34044-34052 NN denotes homology
T19739 34041-34043 IN denotes of
T19738 34031-34036 JJ denotes short
T19737 34037-34040 NN denotes arm
T19736 34027-34030 DT denotes the
T19735 34019-34026 IN denotes outside
T19734 34011-34018 VBN denotes located
T19733 34009-34011 , denotes ,
T19732 33995-33996 -RRB- denotes )
T19731 33994-33995 CD denotes 4
T19730 33987-33993 NN denotes intron
T19729 33986-33987 -LRB- denotes (
T19728 33997-34002 NN denotes sense
T19727 33979-33985 JJ denotes common
T19726 34003-34009 NN denotes primer
T19725 33977-33978 DT denotes a
T19724 33971-33976 VBG denotes using
T19723 33961-33970 NNS denotes reactions
T19722 33952-33960 JJ denotes separate
T19721 33949-33951 IN denotes in
T19720 33934-33938 VBD denotes were
T19719 33920-33926 NN denotes mutant
T19718 33927-33933 NN denotes allele
T19717 33916-33919 DT denotes the
T19716 33913-33915 IN denotes of
T19715 33902-33904 NN denotes bp
T19714 33896-33901 CD denotes 2,711
T19713 33905-33912 NN denotes segment
T19712 33894-33895 DT denotes a
T19711 33890-33893 CC denotes and
T19710 33877-33878 HYPH denotes -
T19709 33878-33882 NN denotes type
T19708 33873-33877 JJ denotes wild
T19707 33883-33889 NN denotes allele
T19706 33869-33872 DT denotes the
T19705 33866-33868 IN denotes of
T19704 33939-33948 VBN denotes amplified
T19703 33855-33857 NN denotes bp
T19702 33849-33854 CD denotes 2,748
T19701 33858-33865 NN denotes segment
T19700 33847-33848 DT denotes A
T19699 33846-34170 sentence denotes A 2,748 bp segment of the wild-type allele and a 2,711 bp segment of the mutant allele were amplified in separate reactions using a common (intron 4) sense primer, located outside the short arm of homology, and distinct antisense primers which hybridized to either wild-type (exon 7) or mutant (PGK-Neo) sequence (Table I).
T19698 33845-33846 . denotes .
T19697 33844-33845 -RRB- denotes )
T19696 33843-33844 CD denotes 1
T19695 33836-33842 NN denotes Figure
T19694 33835-33836 -LRB- denotes (
T19693 33825-33834 NNS denotes weanlings
T19692 33821-33824 CC denotes and
T19691 33813-33820 NNS denotes embryos
T19690 33795-33812 JJ denotes post-implantation
T19689 33790-33794 IN denotes from
T19688 33781-33789 NNS denotes biopsies
T19687 33778-33780 CC denotes or
T19686 33772-33777 NNS denotes cells
T19685 33769-33771 NN denotes ES
T19684 33758-33768 VBG denotes genotyping
T19683 33754-33757 IN denotes for
T19682 33743-33753 JJ denotes sufficient
T19681 33739-33742 VBD denotes was
T19680 33726-33729 NN denotes PCR
T19679 33720-33721 HYPH denotes -
T19678 33721-33725 NN denotes step
T19677 33714-33720 JJ denotes single
T19676 33730-33738 NN denotes strategy
T19675 33712-33713 DT denotes A
T19674 33711-33846 sentence denotes A single-step PCR strategy was sufficient for genotyping ES cells or biopsies from post-implantation embryos and weanlings (Figure 1).
T19673 33710-33711 . denotes .
T19672 33709-33710 CD denotes 1
T19671 33703-33708 NN denotes Table
T19670 33700-33702 IN denotes in
T19669 33689-33692 VBP denotes are
T19668 33665-33680 NN denotes oligonucleotide
T19667 33681-33688 NNS denotes primers
T19666 33661-33664 DT denotes all
T19665 33658-33660 IN denotes of
T19664 33693-33699 VBN denotes listed
T19663 33648-33657 NNS denotes sequences
T19662 33644-33647 DT denotes The
T19661 33643-33711 sentence denotes The sequences of all oligonucleotide primers are listed in Table 1.
T19660 33642-33643 . denotes .
T19659 33639-33642 NN denotes DNA
T19658 33631-33638 JJ denotes genomic
T19657 33628-33630 IN denotes of
T19656 33619-33627 NN denotes analysis
T19655 33615-33618 NN denotes PCR
T19654 33612-33614 IN denotes by
T19653 33608-33611 RP denotes out
T19652 33595-33599 RB denotes also
T19651 33591-33594 VBD denotes was
T19650 33600-33607 VBN denotes carried
T19649 33580-33590 NN denotes Genotyping
T19648 33579-33643 sentence denotes Genotyping was also carried out by PCR analysis of genomic DNA.
T19647 33578-33579 . denotes .
T19646 33572-33578 NNS denotes clones
T19645 33563-33571 VBN denotes targeted
T19644 33560-33562 IN denotes in
T19643 33542-33553 NN denotes integration
T19642 33535-33541 JJ denotes single
T19641 33554-33559 NN denotes event
T19640 33533-33534 DT denotes a
T19639 33526-33532 VB denotes verify
T19638 33523-33525 TO denotes to
T19637 33517-33522 NN denotes order
T19636 33514-33516 IN denotes in
T19635 33508-33513 NNS denotes blots
T19634 33499-33507 NNP denotes Southern
T19633 33493-33498 VB denotes probe
T19632 33490-33492 TO denotes to
T19631 33480-33484 RB denotes also
T19630 33476-33479 VBD denotes was
T19629 33462-33463 HYPH denotes -
T19628 33463-33466 NN denotes Neo
T19627 33459-33462 NN denotes PGK
T19626 33467-33475 NN denotes cassette
T19625 33455-33458 DT denotes the
T19624 33450-33454 IN denotes from
T19623 33485-33489 VBN denotes used
T19622 33435-33436 HYPH denotes -
T19621 33436-33440 NN denotes XbaI
T19620 33431-33435 NN denotes PstI
T19619 33428-33430 NN denotes bp
T19618 33424-33427 CD denotes 681
T19617 33441-33449 NN denotes fragment
T19616 33422-33423 DT denotes A
T19615 33421-33579 sentence denotes A 681 bp PstI-XbaI fragment from the PGK-Neo cassette was also used to probe Southern blots in order to verify a single integration event in targeted clones.
T19614 33420-33421 . denotes .
T19613 33419-33420 -RRB- denotes )
T19612 33418-33419 CD denotes 1
T19611 33411-33417 NN denotes Figure
T19610 33410-33411 -LRB- denotes (
T19609 33401-33409 NN denotes homology
T19608 33398-33400 IN denotes of
T19607 33388-33393 JJ denotes short
T19606 33394-33397 NN denotes arm
T19605 33384-33387 DT denotes the
T19604 33381-33383 IN denotes of
T19603 33372-33380 RB denotes upstream
T19602 33360-33371 RB denotes immediately
T19601 33352-33359 VBN denotes located
T19600 33334-33335 HYPH denotes -
T19599 33335-33342 NN denotes HindIII
T19598 33329-33334 NN denotes BamHI
T19597 33317-33318 HYPH denotes -
T19596 33316-33317 CD denotes 4
T19595 33318-33328 VBG denotes containing
T19594 33311-33315 NN denotes exon
T19593 33308-33310 NN denotes bp
T19592 33304-33307 CD denotes 823
T19591 33295-33296 HYPH denotes -
T19590 33296-33303 VBN denotes labeled
T19589 33292-33295 NN denotes DIG
T19588 33343-33351 NN denotes fragment
T20207 37338-37340 NN denotes °C
T20206 37336-37338 CD denotes 95
T20205 37333-37335 IN denotes at
T20204 37313-37319 NN denotes minute
T20203 37320-37332 NN denotes denaturation
T20202 37309-37312 CD denotes one
T20201 37306-37308 IN denotes of
T20200 37299-37305 NNS denotes cycles
T20199 37296-37298 CD denotes 35
T20198 37294-37296 , denotes ,
T20197 37292-37294 NN denotes °C
T20196 37290-37292 CD denotes 95
T20195 37287-37289 IN denotes at
T20194 37267-37273 NN denotes minute
T20193 37262-37266 CD denotes five
T20192 37254-37261 JJ denotes initial
T20191 37274-37286 NN denotes denaturation
T20190 37251-37253 DT denotes an
T20189 37241-37250 VBG denotes entailing
T20188 37226-37229 NN denotes PCR
T20187 37230-37240 NNS denotes conditions
T20186 37216-37225 JJ denotes identical
T20185 37210-37215 VBG denotes using
T20184 37206-37209 RP denotes out
T20183 37193-37197 VBD denotes were
T20182 37198-37205 VBN denotes carried
T20181 37183-37192 NNS denotes reactions
T20180 37179-37182 DT denotes All
T20179 37178-37449 sentence denotes All reactions were carried out using identical PCR conditions entailing an initial five minute denaturation at 95°C, 35 cycles of one minute denaturation at 95°C, one minute annealing at 56°C, and one minute extension at 72°C, with a final extension step of ten minutes.
T20178 37177-37178 . denotes .
T20177 37167-37173 NN denotes mutant
T20176 37174-37177 NN denotes PCR
T20175 37163-37166 DT denotes the
T20174 37159-37162 IN denotes for
T20173 37156-37158 NN denotes bp
T20172 37152-37155 CD denotes 389
T20171 37148-37151 CC denotes and
T20170 37146-37148 , denotes ,
T20169 37137-37138 HYPH denotes -
T20168 37138-37142 NN denotes type
T20167 37133-37137 JJ denotes wild
T20166 37143-37146 NN denotes PCR
T20165 37129-37132 DT denotes the
T20164 37125-37128 IN denotes for
T20163 37122-37124 NN denotes bp
T20162 37118-37121 CD denotes 429
T20161 37116-37118 , denotes ,
T20160 37105-37112 NN denotes control
T20159 37113-37116 NN denotes PCR
T20158 37101-37104 DT denotes the
T20157 37097-37100 IN denotes for
T20156 37094-37096 NN denotes bp
T20155 37090-37093 CD denotes 213
T20154 37085-37089 VBD denotes were
T20153 37070-37075 JJ denotes final
T20152 37076-37084 NNS denotes products
T20151 37066-37069 DT denotes The
T20150 37065-37178 sentence denotes The final products were 213 bp for the control PCR, 429 bp for the wild-type PCR, and 389 bp for the mutant PCR.
T20149 37064-37065 . denotes .
T20148 37048-37057 NN denotes antisense
T20147 37046-37047 CD denotes 5
T20146 37039-37045 NN denotes intron
T20145 37032-37038 VBN denotes nested
T20144 37058-37064 NN denotes primer
T20143 37030-37031 DT denotes a
T20142 37026-37029 CC denotes and
T20141 37013-37018 NN denotes sense
T20140 37011-37012 CD denotes 5
T20139 37006-37010 NN denotes exon
T20138 37019-37025 NN denotes primer
T20137 37003-37005 DT denotes an
T20136 36993-37002 VBD denotes comprised
T20135 36981-36987 NN denotes primer
T20134 36974-36980 JJ denotes second
T20133 36988-36992 NN denotes pair
T20132 36970-36973 DT denotes The
T20131 36969-37065 sentence denotes The second primer pair comprised an exon 5 sense primer and a nested intron 5 antisense primer.
T20130 36968-36969 . denotes .
T20129 36952-36961 NN denotes antisense
T20128 36950-36951 CD denotes 5
T20127 36943-36949 NN denotes intron
T20126 36962-36968 NN denotes primer
T20125 36940-36942 DT denotes an
T20124 36936-36939 CC denotes and
T20123 36923-36928 NN denotes sense
T20122 36921-36922 CD denotes 4
T20121 36914-36920 NN denotes intron
T20120 36929-36935 NN denotes primer
T20119 36911-36913 DT denotes an
T20118 36908-36910 IN denotes of
T20117 36905-36907 RP denotes up
T20116 36896-36899 VBD denotes was
T20115 36884-36891 NN denotes control
T20114 36892-36895 NN denotes PCR
T20113 36880-36883 DT denotes the
T20112 36877-36879 IN denotes of
T20111 36900-36904 VBN denotes made
T20110 36865-36871 NN denotes primer
T20109 36859-36864 JJ denotes first
T20108 36872-36876 NN denotes pair
T20107 36855-36858 DT denotes The
T20106 36854-36969 sentence denotes The first primer pair of the control PCR was made up of an intron 4 sense primer and an intron 5 antisense primer.
T20105 36853-36854 . denotes .
T20104 36839-36845 NN denotes mutant
T20103 36835-36838 CC denotes and
T20102 36829-36830 HYPH denotes -
T20101 36830-36834 NN denotes type
T20100 36825-36829 JJ denotes wild
T20099 36821-36824 DT denotes the
T20098 36846-36853 NNS denotes alleles
T20097 36816-36820 CC denotes both
T20096 36813-36815 IN denotes in
T20095 36800-36802 VBZ denotes is
T20094 36803-36812 VBN denotes preserved
T20093 36795-36799 WDT denotes that
T20092 36778-36787 NN denotes targeting
T20091 36788-36794 NN denotes vector
T20090 36774-36777 DT denotes the
T20089 36771-36773 IN denotes of
T20088 36761-36766 JJ denotes short
T20087 36767-36770 NN denotes arm
T20086 36757-36760 DT denotes the
T20085 36750-36756 IN denotes within
T20084 36735-36742 JJ denotes genomic
T20083 36743-36749 NN denotes region
T20082 36733-36734 DT denotes a
T20081 36725-36732 VB denotes amplify
T20080 36722-36724 TO denotes to
T20079 36713-36721 VBN denotes designed
T20078 36696-36704 JJ denotes internal
T20077 36689-36695 JJ denotes common
T20076 36705-36712 NN denotes control
T20075 36687-36688 DT denotes a
T20074 36668-36677 NNS denotes reactions
T20073 36665-36667 IN denotes of
T20072 36656-36659 CD denotes two
T20071 36660-36664 NNS denotes sets
T20070 36652-36655 DT denotes the
T20069 36650-36652 , denotes ,
T20068 36642-36650 NN denotes addition
T20067 36678-36686 VBD denotes included
T19587 33290-33291 DT denotes a
T19586 33285-33289 IN denotes with
T19585 33274-33284 VBD denotes hybridized
T19584 33270-33273 CC denotes and
T19583 33258-33265 JJ denotes genomic
T19582 33266-33269 NN denotes DNA
T19581 33248-33249 HYPH denotes -
T19580 33249-33257 VBN denotes digested
T19579 33243-33248 NN denotes BamHI
T19578 33238-33242 IN denotes with
T19577 33225-33229 VBD denotes were
T19576 33215-33224 NNS denotes membranes
T19575 33213-33215 , denotes ,
T19574 33208-33213 NNS denotes cases
T19573 33203-33207 JJS denotes most
T19572 33230-33237 VBN denotes blotted
T19571 33200-33202 IN denotes In
T19570 33199-33421 sentence denotes In most cases, membranes were blotted with BamHI-digested genomic DNA and hybridized with a DIG-labeled 823 bp exon 4-containing BamHI-HindIII fragment located immediately upstream of the short arm of homology (Figure 1).
T19569 33198-33199 . denotes .
T19568 33197-33198 -RRB- denotes )
T19567 33192-33197 NNP denotes Roche
T19566 33191-33192 -LRB- denotes (
T19565 33168-33183 JJ denotes non-radioactive
T19564 33166-33167 -RRB- denotes )
T19563 33163-33166 NN denotes DIG
T19562 33162-33163 -LRB- denotes (
T19561 33150-33161 NN denotes digoxigenin
T19560 33184-33190 NN denotes system
T19559 33146-33149 DT denotes the
T19558 33140-33145 VBG denotes using
T19557 33136-33139 RP denotes out
T19556 33124-33127 VBD denotes was
T19555 33128-33135 VBN denotes carried
T19554 33115-33123 NN denotes blotting
T19553 33106-33114 NNP denotes Southern
T19850 35573-35578 JJ denotes Whole
T19849 35572-35651 sentence denotes Whole embryos were first digested in 20 μL of proteinase K buffer (see below).
T19848 35571-35572 . denotes .
T19847 35570-35571 -RRB- denotes )
T19846 35569-35570 CD denotes 4
T19845 35562-35568 NN denotes Figure
T19844 35561-35562 -LRB- denotes (
T19843 35538-35548 NN denotes genotyping
T19842 35549-35560 NN denotes information
T19841 35529-35537 JJ denotes reliable
T19840 35523-35528 VB denotes yield
T19839 35520-35522 TO denotes to
T19838 35506-35509 VBD denotes was
T19837 35493-35496 NN denotes PCR
T19836 35486-35492 VBN denotes nested
T19835 35497-35505 NN denotes strategy
T19834 35484-35485 DT denotes a
T19833 35482-35484 , denotes ,
T19832 35475-35482 NNS denotes embryos
T19831 35458-35474 JJ denotes pre-implantation
T19830 35455-35457 IN denotes in
T19829 35445-35454 JJ denotes available
T19828 35436-35444 NN denotes material
T19827 35428-35435 JJ denotes genetic
T19826 35425-35427 IN denotes of
T19825 35410-35417 VBN denotes limited
T19824 35418-35424 NN denotes amount
T19823 35406-35409 DT denotes the
T19822 35403-35405 IN denotes to
T19821 35510-35519 VBN denotes developed
T19820 35399-35402 IN denotes Due
T19819 35399-35572 sentence denotes Due to the limited amount of genetic material available in pre-implantation embryos, a nested PCR strategy was developed to yield reliable genotyping information (Figure 4).
T19818 34407-34408 sentence denotes
T19817 34406-34407 . denotes .
T19816 34392-34401 NN denotes extension
T19815 34386-34391 JJ denotes final
T19814 34379-34385 NN denotes minute
T19813 34375-34378 CD denotes ten
T19812 34402-34406 NN denotes step
T19811 34373-34374 DT denotes a
T19810 34368-34372 IN denotes with
T19809 34366-34368 , denotes ,
T19808 34364-34366 NN denotes °C
T19807 34362-34364 CD denotes 72
T19806 34359-34361 IN denotes at
T19805 34349-34358 NN denotes extension
T19804 34342-34348 NN denotes minute
T19793 34300-34302 NN denotes °C
T19792 34298-34300 CD denotes 95
T19791 34295-34297 IN denotes at
T19790 34282-34294 NN denotes denaturation
T19789 34275-34281 NN denotes minute
T19788 34271-34274 CD denotes one
T19787 34268-34270 IN denotes of
T19786 34261-34267 NNS denotes cycles
T19785 34258-34260 CD denotes 30
T19784 34256-34258 , denotes ,
T19783 34254-34256 NN denotes °C
T19782 34252-34254 CD denotes 95
T19781 34249-34251 IN denotes at
T19780 34231-34243 NN denotes denaturation
T19779 34223-34230 JJ denotes initial
T19778 34216-34222 NN denotes minute
T19777 34211-34215 CD denotes five
T19776 34244-34248 NN denotes step
T19775 34209-34210 DT denotes a
T19774 34200-34208 VBD denotes included
T19773 34175-34188 VBG denotes thermocycling
T19772 34189-34199 NNS denotes parameters
T19771 34171-34174 DT denotes The
T19770 34170-34407 sentence denotes The thermocycling parameters included a five minute initial denaturation step at 95°C, 30 cycles of one minute denaturation at 95°C, one minute annealing at 56°C, and one minute extension at 72°C, with a ten minute final extension step.
T19769 34169-34170 . denotes .
T19552 33105-33199 sentence denotes Southern blotting was carried out using the digoxigenin (DIG) non-radioactive system (Roche).
T19551 33104-33105 . denotes .
T19550 33093-33098 NN denotes Capn2
T19549 33099-33104 NN denotes locus
T19548 33089-33092 DT denotes the
T19547 33086-33088 IN denotes of
T19546 33077-33085 NN denotes genotype
T19545 33073-33076 DT denotes the
T19544 33063-33072 VB denotes determine
T19543 33060-33062 TO denotes to
T19542 33054-33059 NN denotes order
T19541 33051-33053 IN denotes in
T19540 33036-33040 VBD denotes were
T19539 33025-33035 NNS denotes strategies
T19538 33021-33024 NN denotes PCR
T19537 33017-33020 CC denotes and
T19536 33041-33050 VBN denotes exploited
T19535 33003-33011 NNP denotes Southern
T19534 33012-33016 NN denotes blot
T19533 32995-33002 JJ denotes Several
T19532 32994-33105 sentence denotes Several Southern blot and PCR strategies were exploited in order to determine the genotype of the Capn2 locus.
T19531 32987-32994 NNS denotes methods
T19530 32976-32986 NN denotes Genotyping
T18112 32973-32974 . denotes .
T18111 32969-32973 NNP denotes Care
T18110 32962-32968 NNP denotes Animal
T18109 32959-32961 IN denotes on
T18108 32942-32950 NNP denotes Canadian
T18107 32951-32958 NNP denotes Council
T18106 32938-32941 DT denotes the
T18105 32935-32937 IN denotes of
T18104 32924-32934 NNS denotes guidelines
T18103 32920-32923 DT denotes the
T18102 32917-32919 IN denotes to
T18101 32907-32916 VBG denotes according
T18100 32892-32896 NNP denotes Care
T18099 32885-32891 NNP denotes Animal
T18098 32897-32906 NNP denotes Committee
T18097 32871-32873 POS denotes 's
T18096 32874-32884 NNP denotes University
T18095 32866-32871 NNP denotes Queen
T18094 32862-32865 DT denotes the
T18093 32859-32861 IN denotes by
T18092 32845-32849 VBD denotes were
T18091 32850-32858 VBN denotes approved
T18090 32835-32844 NNS denotes protocols
T18089 32829-32834 NN denotes Mouse
T18088 32828-32974 sentence denotes Mouse protocols were approved by the Queen's University Animal Care Committee according to the guidelines of the Canadian Council on Animal Care.
T17710 32277-32278 -RRB- denotes )
T17709 32272-32277 RB denotes below
T17708 32268-32271 VB denotes see
T17707 32267-32268 -LRB- denotes (
T17706 32258-32266 NN denotes analysis
T17705 32254-32257 NN denotes PCR
T17704 32250-32253 CC denotes and
T17703 32241-32249 NN denotes blotting
T17702 32232-32240 NNP denotes Southern
T17701 32229-32231 IN denotes by
T17700 32219-32228 VBN denotes genotyped
T17699 32215-32218 CC denotes and
T17698 32213-32215 , denotes ,
T17697 32207-32213 NNS denotes plates
T17696 32199-32200 HYPH denotes -
T17695 32200-32206 VBN denotes coated
T17694 32192-32199 NN denotes gelatin
T17693 32189-32191 IN denotes on
T17692 32180-32188 VBN denotes expanded
T17691 32178-32180 , denotes ,
T17690 32167-32171 VBD denotes were
T17689 32172-32178 VBN denotes picked
T17688 32160-32166 NNS denotes clones
T17687 32149-32150 HYPH denotes -
T17686 32150-32159 JJ denotes resistant
T17685 32145-32149 NN denotes Drug
T17684 32144-32279 sentence denotes Drug-resistant clones were picked, expanded on gelatin-coated plates, and genotyped by Southern blotting and PCR analysis (see below).
T17614 31795-31798 DT denotes all
T17613 31793-31795 , denotes ,
T17612 31829-31833 VBD denotes were
T17611 31774-31783 RB denotes otherwise
T17610 31784-31793 VBN denotes specified
T17609 31767-31773 IN denotes Unless
T17608 31766-31849 sentence denotes Unless otherwise specified, all other tissue culture reagents were from Gibco-BRL.
T17607 31765-31766 . denotes .
T17606 31764-31765 -RRB- denotes )
T17605 31757-31764 NNP denotes Ontario
T17604 31755-31757 , denotes ,
T17603 31747-31755 NNP denotes Oakville
T17602 31746-31747 -LRB- denotes (
T17601 31739-31745 NNP denotes Canada
T17600 31730-31731 HYPH denotes -
T17599 31731-31738 NNP denotes Aldrich
T17598 31725-31730 NNP denotes Sigma
T17597 31720-31724 IN denotes from
T17596 31716-31719 VBD denotes was
T17595 31708-31715 NN denotes Gelatin
T17594 31707-31766 sentence denotes Gelatin was from Sigma-Aldrich Canada (Oakville, Ontario).
T17593 31706-31707 . denotes .
T17592 31700-31706 NN denotes growth
T17591 31695-31699 NN denotes cell
T17590 31692-31694 NN denotes ES
T17589 31684-31691 VB denotes support
T17588 31681-31683 TO denotes to
T17587 31673-31680 NN denotes ability
T17586 31669-31672 PRP$ denotes its
T17585 31665-31668 IN denotes for
T17584 31654-31657 VBD denotes was
T17583 31652-31653 -RRB- denotes )
T17582 31648-31652 NNP denotes Utah
T17581 31646-31648 , denotes ,
T17580 31641-31646 NNP denotes Logan
T17579 31640-31641 -LRB- denotes (
T17578 31623-31635 NNP denotes Laboratories
T17577 31636-31639 NNP denotes Inc
T17576 31615-31622 NNP denotes HyClone
T17575 31610-31614 IN denotes from
T17574 31658-31664 VBN denotes tested
T17573 31597-31603 JJ denotes bovine
T17572 31604-31609 NN denotes serum
T17571 31591-31596 JJ denotes Fetal
T17570 31590-31707 sentence denotes Fetal bovine serum from HyClone Laboratories Inc (Logan, Utah) was tested for its ability to support ES cell growth.
T17569 31589-31590 . denotes .
T17568 31588-31589 -RRB- denotes )
T17567 31587-31588 -RRB- denotes ]
T17566 31579-31587 NNP denotes Chemicon
T17565 31578-31579 -LRB- denotes [
T17564 31572-31577 NN denotes ESGro
T17563 31569-31571 IN denotes of
T17562 31566-31568 NN denotes ml
T17561 31565-31566 SYM denotes /
T17560 31564-31565 NN denotes U
T17559 31558-31563 CD denotes 1,000
T17558 31554-31557 CC denotes and
T17557 31552-31554 , denotes ,
T17556 31537-31539 NN denotes ml
T17555 31536-31537 SYM denotes /
T17554 31540-31552 NN denotes amphotericin
T17553 31534-31536 NN denotes μg
T17552 31531-31533 CD denotes 25
T17551 31529-31531 , denotes ,
T17550 31514-31516 NN denotes ml
T17549 31513-31514 SYM denotes /
T17548 31517-31529 NN denotes streptomycin
T17547 31511-31513 NN denotes μg
T17546 31507-31510 CD denotes 100
T17545 31505-31507 , denotes ,
T17544 31492-31494 NN denotes ml
T17543 31491-31492 SYM denotes /
T17542 31495-31505 NN denotes penicillin
T17541 31490-31491 NN denotes U
T17540 31486-31489 CD denotes 100
T17539 31484-31486 , denotes ,
T17538 31468-31469 HYPH denotes -
T17537 31467-31468 CD denotes 2
T17536 31469-31484 NN denotes mercaptoethanol
T17535 31464-31466 NN denotes mM
T17534 31460-31463 CD denotes 0.1
T17533 31458-31460 , denotes ,
T17532 31443-31449 NN denotes sodium
T17531 31450-31458 NN denotes pyruvate
T17530 31440-31442 NN denotes mM
T17529 31438-31439 CD denotes 1
T17528 31436-31438 , denotes ,
T17527 31427-31436 NN denotes glutamine
T17526 31424-31426 NN denotes mM
T17525 31422-31423 CD denotes 2
T17524 31420-31422 , denotes ,
T17523 31409-31414 NN denotes amino
T17522 31395-31408 JJ denotes non-essential
T17521 31415-31420 NNS denotes acids
T17520 31392-31394 NN denotes mM
T17519 31388-31391 CD denotes 0.1
T17518 31386-31388 , denotes ,
T17517 31374-31380 JJ denotes bovine
T17516 31368-31373 JJ denotes fetal
T17515 31381-31386 NN denotes serum
T17514 31366-31367 NN denotes %
T17513 31364-31366 CD denotes 15
T17512 31359-31363 IN denotes with
T17511 31346-31358 VBN denotes supplemented
T17510 31344-31345 -RRB- denotes ]
T17509 31332-31336 JJ denotes high
T17508 31337-31344 NN denotes glucose
T17507 31331-31332 -LRB- denotes [
T17506 31326-31330 NN denotes DMEM
T17505 31325-31326 -LRB- denotes (
T17504 31313-31317 NN denotes cell
T17503 31318-31324 NN denotes medium
T17502 31310-31312 NN denotes ES
T17501 31307-31309 IN denotes in
T17500 31303-31306 NN denotes CO2
T17499 31301-31302 NN denotes %
T17498 31300-31301 CD denotes 5
T17497 31294-31299 IN denotes under
T17496 31291-31293 NN denotes °C
T17495 31289-31291 CD denotes 37
T17494 31286-31288 IN denotes at
T17493 31264-31273 JJ denotes embryonic
T17492 31274-31285 NNS denotes fibroblasts
T17491 31258-31263 NN denotes mouse
T17490 31255-31257 IN denotes of
T17489 31248-31254 NNS denotes layers
T17488 31241-31247 NN denotes feeder
T17487 31236-31240 IN denotes with
T17486 31229-31235 NNS denotes plates
T17485 31221-31222 HYPH denotes -
T17484 31222-31228 VBN denotes coated
T17483 31214-31221 NN denotes gelatin
T17482 31211-31213 IN denotes on
T17481 31195-31199 VBD denotes were
T17480 31193-31194 -RRB- denotes ]
T17479 31191-31193 CD denotes 49
T17478 31190-31191 -LRB- denotes [
T17477 31200-31210 VBN denotes maintained
T17476 31181-31183 NN denotes ES
T17475 31178-31180 NN denotes R1
T17474 31184-31189 NNS denotes cells
T17473 31172-31177 NN denotes Mouse
T17472 31171-31590 sentence denotes Mouse R1 ES cells [49] were maintained on gelatin-coated plates with feeder layers of mouse embryonic fibroblasts at 37°C under 5% CO2 in ES cell medium (DMEM [high glucose] supplemented with 15% fetal bovine serum, 0.1 mM non-essential amino acids, 2 mM glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 100 U/ml penicillin, 100 μg/ml streptomycin, 25 μg/ml amphotericin, and 1,000 U/ml of ESGro [Chemicon]).
T17471 31165-31171 NNS denotes clones
T17470 31156-31164 VBN denotes targeted
T17469 31153-31155 IN denotes of
T17468 31143-31152 NN denotes selection
T17467 31139-31142 CC denotes and
T17466 31126-31138 NN denotes transfection
T17465 31124-31126 , denotes ,
T17464 31112-31116 NN denotes cell
T17463 31117-31124 NN denotes culture
T17462 31109-31111 NN denotes ES
T18087 32827-32828 . denotes .
T18086 32809-32816 JJ denotes genetic
T18085 32802-32808 JJ denotes inbred
T18084 32817-32827 NN denotes background
T18083 32799-32801 DT denotes an
T18082 32796-32798 IN denotes in
T18081 32787-32795 NN denotes mutation
T18080 32783-32786 DT denotes the
T18079 32773-32782 VB denotes establish
T18078 32770-32772 TO denotes to
T18077 32762-32769 NNS denotes females
T18076 32755-32761 NN denotes 129SvJ
T18075 32750-32754 IN denotes with
T18074 32740-32744 RB denotes then
T18073 32735-32739 VBD denotes were
T18072 32745-32749 VBN denotes bred
T18071 32729-32734 DT denotes These
T18070 32728-32828 sentence denotes These were then bred with 129SvJ females to establish the mutation in an inbred genetic background.
T18069 32727-32728 . denotes .
T18068 32715-32727 NN denotes transmission
T18067 32706-32714 NN denotes germline
T18066 32703-32705 IN denotes of
T18065 32695-32702 JJ denotes capable
T18064 32689-32694 NNS denotes males
T18063 32683-32688 DT denotes those
T18062 32674-32682 VB denotes identify
T18061 32671-32673 TO denotes to
T18060 32663-32670 NNS denotes females
T18059 32659-32662 NN denotes CD1
T18058 32654-32658 IN denotes with
T18057 32644-32648 VBD denotes were
T18056 32649-32653 VBN denotes bred
T18055 32638-32643 NNS denotes males
T18054 32629-32637 JJ denotes Chimeric
T18053 32628-32728 sentence denotes Chimeric males were bred with CD1 females to identify those males capable of germline transmission.
T18052 32627-32628 . denotes .
T18051 32616-32620 NN denotes coat
T18050 32621-32627 NN denotes colour
T18049 32609-32615 NN denotes agouti
T18048 32606-32608 IN denotes of
T18047 32598-32605 NNS denotes patches
T18046 32595-32597 IN denotes by
T18045 32582-32594 RB denotes subsequently
T18044 32578-32581 CC denotes and
T18043 32561-32564 NN denotes eye
T18042 32565-32577 NN denotes pigmentation
T18041 32555-32560 JJ denotes black
T18040 32552-32554 IN denotes by
T18039 32546-32551 NN denotes birth
T18038 32543-32545 IN denotes at
T18037 32530-32542 JJ denotes identifiable
T18036 32525-32529 VBD denotes were
T18035 32517-32524 NNS denotes animals
T18034 32508-32516 JJ denotes Chimeric
T18033 32507-32628 sentence denotes Chimeric animals were identifiable at birth by black eye pigmentation and subsequently by patches of agouti coat colour.
T18032 32506-32507 . denotes .
T18031 32495-32498 NN denotes CD1
T18030 32499-32506 NNS denotes females
T18029 32480-32494 JJ denotes pseudopregnant
T18028 32477-32479 IN denotes to
T18027 32460-32464 VBD denotes were
T18026 32448-32459 NNS denotes blastocysts
T18025 32446-32448 , denotes ,
T18024 32438-32442 JJ denotes next
T18023 32443-32446 NN denotes day
T18022 32434-32437 DT denotes the
T18021 32465-32476 VBN denotes transferred
T18020 32431-32433 IN denotes On
T18019 32430-32507 sentence denotes On the next day, blastocysts were transferred to pseudopregnant CD1 females.
T18018 32429-32430 . denotes .
T18017 32428-32429 -RRB- denotes ]
T18016 32426-32428 CD denotes 31
T18015 32425-32426 -LRB- denotes [
T18014 32404-32414 RB denotes previously
T18013 32415-32424 VBN denotes described
T18012 32401-32403 IN denotes as
T18011 32399-32401 , denotes ,
T18010 32392-32399 NNS denotes matings
T18009 32388-32391 NN denotes CD1
T18008 32383-32387 IN denotes from
T18007 32373-32382 VBN denotes recovered
T18006 32365-32372 NNS denotes embryos
T18005 32359-32360 HYPH denotes -
T18004 32360-32364 NN denotes cell
T18003 32358-32359 CD denotes 8
T18002 32353-32357 IN denotes with
T18001 32343-32352 RB denotes overnight
T18000 32327-32331 VBD denotes were
T17999 32332-32342 VBN denotes aggregated
T17998 32318-32320 NN denotes ES
T17997 32316-32317 SYM denotes -
T17996 32315-32316 HYPH denotes /
T17995 32314-32315 SYM denotes +
T17994 32321-32326 NNS denotes cells
T17993 32309-32314 NN denotes Capn2
T17992 32308-32430 sentence denotes Capn2+/- ES cells were aggregated overnight with 8-cell embryos recovered from CD1 matings, as previously described [31].
T17991 32304-32308 NNS denotes mice
T17990 32295-32303 VBN denotes targeted
T17989 32292-32294 IN denotes of
T17988 32281-32291 NN denotes Generation
T17683 32143-32144 . denotes .
T17682 32139-32143 NNS denotes days
T17681 32133-32138 CD denotes eight
T17680 32129-32132 IN denotes for
T17679 32127-32128 -RRB- denotes )
T17678 32123-32127 NNP denotes Inc.
T17677 32121-32123 , denotes ,
T17676 32115-32121 NNP denotes Syntex
T17675 32114-32115 -LRB- denotes (
T17674 32102-32113 NN denotes ganciclovir
T17673 32099-32101 NN denotes μM
T17672 32097-32098 CD denotes 2
T17671 32093-32096 CC denotes and
T17670 32091-32092 -RRB- denotes )
T17669 32086-32087 HYPH denotes -
T17668 32081-32086 NNP denotes Gibco
T17667 32088-32091 NNP denotes BRL
T17666 32080-32081 -LRB- denotes (
T17665 32072-32074 NN denotes ml
T17664 32071-32072 SYM denotes /
T17663 32075-32079 NN denotes G418
T17662 32069-32071 NN denotes μg
T17661 32065-32068 CD denotes 200
T17660 32062-32064 IN denotes of
T17659 32053-32061 NN denotes presence
T17658 32049-32052 DT denotes the
T17657 32046-32048 IN denotes in
T17656 32032-32036 VBD denotes were
T17655 32037-32045 VBN denotes selected
T17654 32025-32031 NNS denotes clones
T17653 32013-32024 VBN denotes transformed
T17652 32009-32012 CC denotes and
T17651 32002-32008 NNS denotes plates
T17650 31994-31995 HYPH denotes -
T17649 31995-32001 VBN denotes coated
T17648 31987-31994 NN denotes gelatin
T17647 31984-31986 IN denotes on
T17646 31977-31983 NNS denotes layers
T17645 31970-31976 NN denotes feeder
T17644 31962-31969 IN denotes without
T17643 31950-31954 VBD denotes were
T17642 31955-31961 VBN denotes plated
T17641 31944-31949 NNS denotes Cells
T17640 31943-32144 sentence denotes Cells were plated without feeder layers on gelatin-coated plates and transformed clones were selected in the presence of 200 μg/ml G418 (Gibco- BRL) and 2 μM ganciclovir (Syntex, Inc.) for eight days.
T17639 31942-31943 . denotes .
T17638 31934-31936 NN denotes ES
T17637 31937-31942 NNS denotes cells
T17636 31931-31933 NN denotes R1
T17635 31926-31930 IN denotes into
T17634 31911-31925 VBN denotes electroporated
T17633 31907-31910 CC denotes and
T17632 31897-31906 NN denotes digestion
T17631 31892-31896 NN denotes NotI
T17630 31889-31891 IN denotes by
T17629 31874-31877 VBD denotes was
T17628 31878-31888 VBN denotes linearized
T17627 31854-31863 NN denotes targeting
T17626 31864-31873 NN denotes construct
T17625 31850-31853 DT denotes The
T17624 31849-31943 sentence denotes The targeting construct was linearized by NotI digestion and electroporated into R1 ES cells.
T17623 31848-31849 . denotes .
T17622 31844-31845 HYPH denotes -
T17621 31845-31848 NNP denotes BRL
T17620 31839-31844 NNP denotes Gibco
T17619 31834-31838 IN denotes from
T17618 31812-31819 NN denotes culture
T17617 31805-31811 NN denotes tissue
T17616 31799-31804 JJ denotes other
T17615 31820-31828 NNS denotes reagents
T16892 29825-29829 JJ denotes long
T16891 29843-29846 NN denotes arm
T16890 29821-29824 DT denotes The
T16889 29820-30008 sentence denotes The long (downstream) arm of homology was provided by a 7-kb HindIII-KpnI fragment, extending from intron 7 to intron 12, inserted between the PGK-Neo and thymidine kinase (tk) cassettes.
T16888 29819-29820 . denotes .
T16887 29811-29819 NN denotes blotting
T16886 29802-29810 NNP denotes Southern
T16885 29799-29801 IN denotes by
T16884 29784-29790 NN denotes mutant
T16883 29780-29783 CC denotes and
T16882 29774-29775 HYPH denotes -
T16881 29775-29779 NN denotes type
T16880 29770-29774 JJ denotes wild
T16879 29791-29798 NNS denotes alleles
T16878 29766-29769 DT denotes the
T16877 29751-29765 VBG denotes distinguishing
T16876 29747-29750 IN denotes for
T16875 29741-29746 NN denotes basis
T16874 29739-29740 DT denotes a
T16873 29728-29730 , denotes ,
T16872 29715-29716 HYPH denotes -
T16871 29716-29719 NN denotes Neo
T16870 29712-29715 NN denotes PGK
T16869 29720-29728 NN denotes cassette
T16868 29708-29711 DT denotes the
T16867 29705-29707 IN denotes of
T16866 29699-29700 SYM denotes '
T16865 29698-29699 CD denotes 3
T16864 29701-29704 NN denotes end
T16863 29694-29697 DT denotes the
T16862 29691-29693 IN denotes at
T16861 29680-29685 NN denotes BamHI
T16860 29676-29679 JJ denotes new
T16859 29686-29690 NN denotes site
T16858 29674-29675 DT denotes a
T16857 29671-29673 IN denotes of
T16856 29658-29670 NN denotes introduction
T16855 29654-29657 DT denotes the
T16854 29649-29653 IN denotes with
T16853 29641-29648 VBN denotes coupled
T16852 29639-29641 , denotes ,
T16851 29626-29632 NN denotes mutant
T16850 29633-29639 NN denotes allele
T16849 29622-29625 DT denotes the
T16848 29619-29621 IN denotes in
T16847 29608-29613 NN denotes BamHI
T16846 29614-29618 NN denotes site
T16845 29603-29607 DT denotes this
T16844 29600-29602 IN denotes of
T16843 29730-29738 VBN denotes provided
T16842 29595-29599 NN denotes loss
T16841 29591-29594 DT denotes The
T16840 29590-29820 sentence denotes The loss of this BamHI site in the mutant allele, coupled with the introduction of a new BamHI site at the 3' end of the PGK-Neo cassette, provided a basis for distinguishing the wild-type and mutant alleles by Southern blotting.
T16839 29589-29590 . denotes .
T16838 29576-29579 VBD denotes was
T16837 29566-29571 JJ denotes short
T16836 29572-29575 NN denotes arm
T16835 29562-29565 DT denotes the
T16834 29559-29561 IN denotes of
T16833 29553-29554 SYM denotes '
T16832 29552-29553 CD denotes 3
T16831 29555-29558 NN denotes end
T16830 29548-29551 DT denotes the
T16829 29545-29547 IN denotes at
T16828 29534-29539 NN denotes BamHI
T16827 29540-29544 NN denotes site
T16826 29530-29533 DT denotes the
T16825 29528-29530 , denotes ,
T16824 29521-29528 NN denotes cloning
T16823 29580-29589 VBN denotes abolished
T16822 29514-29520 IN denotes During
T16821 29513-29590 sentence denotes During cloning, the BamHI site at the 3' end of the short arm was abolished.
T16820 29512-29513 . denotes .
T16819 29511-29512 -RRB- denotes ]
T16818 29509-29511 CD denotes 48
T16817 29508-29509 -LRB- denotes [
T16816 29494-29495 HYPH denotes -
T16815 29495-29498 NN denotes Neo
T16814 29491-29494 NN denotes PGK
T16813 29499-29507 NN denotes cassette
T16812 29487-29490 DT denotes the
T16811 29484-29486 IN denotes of
T16810 29475-29483 RB denotes upstream
T16809 29463-29467 NN denotes pPNT
T16808 29468-29474 NN denotes vector
T16807 29459-29462 DT denotes the
T16806 29454-29458 IN denotes into
T16805 29441-29444 VBD denotes was
T16804 29445-29453 VBN denotes inserted
T16803 29435-29440 WDT denotes which
T16802 29433-29435 , denotes ,
T16801 29432-29433 CD denotes 6
T16800 29428-29431 CC denotes and
T16799 29426-29427 CD denotes 5
T16798 29420-29425 NNS denotes exons
T16797 29409-29419 VBG denotes containing
T16796 29407-29409 , denotes ,
T16795 29392-29393 HYPH denotes -
T16794 29393-29398 NN denotes BamHI
T16793 29385-29392 NN denotes HindIII
T16792 29381-29382 HYPH denotes -
T16791 29382-29384 NN denotes kb
T16790 29378-29381 CD denotes 2.7
T16789 29399-29407 NN denotes fragment
T16788 29376-29377 DT denotes a
T16787 29373-29375 IN denotes by
T16786 29360-29363 VBD denotes was
T16785 29340-29349 NN denotes targeting
T16784 29350-29359 NN denotes construct
T16783 29336-29339 DT denotes the
T16782 29333-29335 IN denotes of
T16781 29364-29372 VBN denotes provided
T16780 29327-29328 -RRB- denotes )
T16779 29319-29327 JJ denotes upstream
T16778 29318-29319 -LRB- denotes (
T16777 29312-29317 JJ denotes short
T16776 29329-29332 NN denotes arm
T16775 29308-29311 DT denotes The
T16774 29307-29513 sentence denotes The short (upstream) arm of the targeting construct was provided by a 2.7-kb HindIII-BamHI fragment, containing exons 5 and 6, which was inserted into the pPNT vector upstream of the PGK-Neo cassette [48].
T16773 29306-29307 . denotes .
T16772 29292-29297 NN denotes triad
T16771 29282-29291 JJ denotes catalytic
T16770 29298-29306 NNS denotes residues
T16769 29278-29281 DT denotes the
T16768 29275-29277 IN denotes of
T16767 29271-29274 CD denotes one
T16766 29269-29271 , denotes ,
T16765 29263-29269 NN denotes Asn286
T16764 29253-29262 VBG denotes including
T16763 29251-29253 , denotes ,
T16762 29239-29240 HYPH denotes -
T16761 29240-29244 NN denotes site
T16760 29233-29239 JJ denotes active
T16759 29245-29251 NN denotes region
T16758 29229-29232 DT denotes the
T16757 29226-29228 IN denotes in
T16756 29214-29219 NN denotes amino
T16755 29220-29225 NNS denotes acids
T16754 29211-29213 CD denotes 24
T16753 29201-29202 CD denotes 7
T16752 29203-29210 VBZ denotes encodes
T16751 29196-29200 NN denotes Exon
T16750 29195-29307 sentence denotes Exon 7 encodes 24 amino acids in the active-site region, including Asn286, one of the catalytic triad residues.
T16749 29194-29195 . denotes .
T16748 29193-29194 -RRB- denotes )
T16747 29192-29193 CD denotes 1
T16746 29185-29191 NN denotes Figure
T16745 29184-29185 -LRB- denotes (
T16744 29170-29171 HYPH denotes -
T16743 29171-29174 NN denotes Neo
T16742 29167-29170 NN denotes PGK
T16741 29175-29183 NN denotes cassette
T16740 29163-29166 DT denotes the
T16739 29158-29162 IN denotes with
T16738 29156-29158 , denotes ,
T16737 29146-29151 NN denotes Capn2
T16736 29152-29156 NN denotes gene
T16735 29142-29145 DT denotes the
T16734 29139-29141 IN denotes of
T16733 29137-29138 CD denotes 7
T16732 29132-29136 NN denotes exon
T16731 29121-29131 VBG denotes containing
T16730 29119-29121 , denotes ,
T16729 29102-29103 HYPH denotes -
T16728 29103-29110 NN denotes HindIII
T16727 29097-29102 NN denotes BamHI
T16726 29094-29096 NN denotes bp
T16725 29090-29093 CD denotes 785
T16724 29111-29119 NN denotes fragment
T16723 29088-29089 DT denotes a
T16722 29080-29087 VB denotes replace
T16721 29077-29079 TO denotes to
T16720 29064-29067 VBD denotes was
T16719 29068-29076 VBN denotes designed
T16718 29044-29053 NN denotes targeting
T16717 29054-29063 NN denotes construct
T16716 29042-29043 DT denotes A
T16715 29041-29195 sentence denotes A targeting construct was designed to replace a 785 bp BamHI-HindIII fragment, containing exon 7 of the Capn2 gene, with the PGK-Neo cassette (Figure 1).
T16714 29025-29034 NN denotes targeting
T16713 29019-29024 NN denotes Capn2
T16712 29035-29041 NN denotes vector
T16711 29015-29018 DT denotes the
T16710 29012-29014 IN denotes of
T16709 28999-29011 NN denotes Construction
T16903 29877-29878 CD denotes 7
T16902 29895-29903 NN denotes fragment
T16901 29875-29876 DT denotes a
T16900 29872-29874 IN denotes by
T16899 29859-29862 VBD denotes was
T16898 29850-29858 NN denotes homology
T16897 29847-29849 IN denotes of
T16896 29863-29871 VBN denotes provided
T16895 29841-29842 -RRB- denotes )
T16894 29831-29841 JJ denotes downstream
T16893 29830-29831 -LRB- denotes (
T16229 28996-28997 . denotes .
T16228 28986-28996 NNS denotes conditions
T16227 28973-28985 JJ denotes non-standard
T16226 28970-28972 IN denotes in
T16225 28959-28969 NN denotes sequencing
T16224 28950-28958 VBN denotes repeated
T16223 28947-28949 IN denotes by
T16222 28928-28934 RB denotes firmly
T16221 28925-28927 VB denotes be
T16220 28920-28924 RB denotes only
T16219 28914-28919 MD denotes could
T16218 28935-28946 VBN denotes established
T16217 28908-28913 WDT denotes which
T16216 28887-28897 JJ denotes repetitive
T16215 28898-28907 NNS denotes sequences
T16214 28881-28886 JJ denotes short
T16213 28878-28880 IN denotes to
T16212 28864-28877 VBG denotes corresponding
T16211 28853-28858 JJ denotes small
T16210 28859-28863 NNS denotes gaps
T16209 28845-28852 JJ denotes several
T16208 28842-28844 RP denotes in
T16207 28835-28841 VBN denotes filled
T16206 28830-28834 RB denotes also
T16205 28826-28829 CC denotes and
T16204 28824-28826 , denotes ,
T16203 28808-28814 JJ denotes public
T16202 28815-28824 NNS denotes databases
T16201 28804-28807 DT denotes the
T16200 28799-28803 IN denotes with
T16199 28789-28798 RB denotes precisely
T16198 28782-28788 VBD denotes agreed
T16197 28762-28771 VBN denotes submitted
T16196 28772-28781 NNS denotes sequences
T16195 28758-28761 DT denotes The
T16194 28757-28997 sentence denotes The submitted sequences agreed precisely with the public databases, and also filled in several small gaps corresponding to short repetitive sequences which could only be firmly established by repeated sequencing in non-standard conditions.
T16193 28756-28757 . denotes .
T16192 28755-28756 -RRB- denotes )
T16191 28743-28746 NN denotes no.
T16190 28733-28742 NN denotes accession
T16189 28747-28755 NN denotes AF497625
T16188 28732-28733 -LRB- denotes (
T16187 28724-28731 NNP denotes GenBank
T16186 28721-28723 IN denotes to
T16185 28706-28710 VBD denotes were
T16184 28711-28720 VBN denotes submitted
T16183 28701-28705 NNS denotes data
T16182 28697-28700 DT denotes the
T16181 28693-28696 CC denotes and
T16180 28685-28692 NNS denotes strands
T16179 28680-28684 DT denotes both
T16178 28677-28679 IN denotes on
T16177 28666-28676 RB denotes completely
T16176 28651-28655 VBD denotes were
T16175 28656-28665 VBN denotes sequenced
T16174 28644-28650 NNS denotes clones
T16173 28638-28643 DT denotes These
T16172 28637-28757 sentence denotes These clones were sequenced completely on both strands and the data were submitted to GenBank (accession no. AF497625).
T16171 28636-28637 . denotes .
T16170 28634-28636 CD denotes 15
T16169 28627-28633 NN denotes intron
T16168 28624-28626 IN denotes in
T16167 28613-28618 NN denotes BamHI
T16166 28619-28623 NN denotes site
T16165 28611-28612 DT denotes a
T16164 28608-28610 IN denotes to
T16163 28606-28607 CD denotes 3
T16162 28599-28605 NN denotes intron
T16161 28596-28598 IN denotes in
T16160 28585-28590 NN denotes BamHI
T16159 28591-28595 NN denotes site
T16158 28583-28584 DT denotes a
T16157 28578-28582 IN denotes from
T16156 28568-28577 VBG denotes extending
T16155 28566-28568 , denotes ,
T16154 28564-28566 NN denotes bp
T16153 28557-28563 CD denotes 15,354
T16152 28548-28556 VBG denotes covering
T16151 28534-28538 VBD denotes were
T16150 28539-28547 VBN denotes obtained
T16149 28519-28526 JJ denotes genomic
T16148 28527-28533 NNS denotes clones
T16147 28507-28518 VBG denotes Overlapping
T16146 28506-28637 sentence denotes Overlapping genomic clones were obtained covering 15,354 bp, extending from a BamHI site in intron 3 to a BamHI site in intron 15.
T16145 28505-28506 . denotes .
T16144 28503-28505 CD denotes II
T16143 28497-28498 HYPH denotes -
T16142 28498-28502 NN denotes Dash
T16141 28496-28497 NN denotes λ
T16140 28493-28495 IN denotes in
T16139 28477-28484 JJ denotes genomic
T16138 28471-28476 NN denotes mouse
T16137 28465-28470 CD denotes 129Sv
T16136 28485-28492 NN denotes library
T16135 28463-28464 DT denotes a
T16134 28456-28462 VB denotes screen
T16133 28453-28455 TO denotes to
T16132 28448-28452 VBN denotes used
T16131 28444-28447 CC denotes and
T16130 28430-28433 VBD denotes was
T16129 28425-28429 NN denotes cDNA
T16128 28420-28424 DT denotes this
T16127 28417-28419 IN denotes of
T16126 28434-28443 VBN denotes subcloned
T16125 28408-28416 NN denotes fragment
T16124 28406-28407 DT denotes A
T16123 28405-28506 sentence denotes A fragment of this cDNA was subcloned and used to screen a 129Sv mouse genomic library in λ-Dash II.
T16122 28404-28405 . denotes .
T16121 28392-28397 NN denotes polyA
T16120 28398-28404 NN denotes signal
T16119 28388-28391 DT denotes the
T16118 28385-28387 IN denotes to
T16117 28380-28381 HYPH denotes -
T16116 28379-28380 SYM denotes '
T16115 28378-28379 CD denotes 3
T16114 28381-28384 NN denotes UTR
T16113 28374-28377 DT denotes the
T16112 28364-28373 IN denotes including
T16111 28362-28364 , denotes ,
T16110 28357-28362 CD denotes 2,101
T16109 28348-28356 NN denotes position
T16108 28345-28347 IN denotes at
T16107 28334-28338 NN denotes stop
T16106 28339-28344 NN denotes codon
T16105 28330-28333 DT denotes the
T16104 28327-28329 IN denotes to
T16103 28311-28317 NN denotes coding
T16102 28318-28326 NN denotes sequence
T16101 28307-28310 DT denotes the
T16100 28304-28306 IN denotes of
T16099 28300-28303 CD denotes 247
T16098 28291-28299 NN denotes position
T16097 28286-28290 IN denotes from
T16096 28277-28285 NN denotes sequence
T16095 28274-28276 IN denotes of
T16094 28270-28271 HYPH denotes -
T16093 28271-28273 NN denotes kb
T16092 28267-28270 CD denotes 2.8
T16091 28259-28266 VB denotes contain
T16090 28256-28258 TO denotes to
T16089 28246-28249 VBD denotes was
T16088 28250-28255 VBN denotes found
T15137 26948-26949 NN denotes %
T15136 26944-26948 CD denotes 22.5
T15135 26942-26944 , denotes ,
T15134 26925-26934 VBN denotes genotyped
T15133 26935-26942 NNS denotes animals
T15132 26922-26924 CD denotes 80
T15131 26919-26921 IN denotes of
T15130 26913-26918 NN denotes total
T15129 26911-26912 DT denotes a
T15128 26908-26910 IN denotes of
T15127 26950-26954 VBD denotes were
T15126 26904-26907 IN denotes Out
T15125 26903-27040 sentence denotes Out of a total of 80 genotyped animals, 22.5% were wild-type and 77.5% were heterozygous, with no homozygous null progeny observed [32].
T15124 26902-26903 . denotes .
T15123 26881-26893 JJ denotes heterozygous
T15122 26894-26902 NN denotes genotype
T15121 26877-26880 DT denotes the
T15120 26874-26876 IN denotes of
T15119 26868-26873 NN denotes favor
T15118 26865-26867 IN denotes in
T15117 26855-26857 , denotes ,
T15116 26843-26847 RBR denotes less
T15115 26840-26842 IN denotes if
T15114 26848-26855 JJ denotes extreme
T15113 26838-26840 , denotes ,
T15112 26831-26838 JJ denotes similar
T15111 26857-26864 VBG denotes skewing
T15110 26829-26830 DT denotes a
T15109 26824-26828 IN denotes with
T15108 26816-26823 NN denotes progeny
T15107 26797-26807 NNS denotes colleagues
T15106 26793-26796 CC denotes and
T15105 26783-26792 NNP denotes Zimmerman
T15104 26780-26782 IN denotes by
T15103 26770-26779 VBN denotes generated
T15102 26754-26764 JJ denotes transgenic
T15101 26748-26753 NN denotes Capn4
T15100 26765-26769 NN denotes line
T15099 26744-26747 DT denotes the
T15098 26734-26743 VBG denotes involving
T15097 26726-26733 NNS denotes crosses
T15096 26713-26725 JJ denotes heterozygous
T15095 26711-26713 , denotes ,
T15094 26808-26815 VBD denotes yielded
T15093 26698-26711 RB denotes Interestingly
T15092 26697-26903 sentence denotes Interestingly, heterozygous crosses involving the Capn4 transgenic line generated by Zimmerman and colleagues yielded progeny with a similar, if less extreme, skewing in favor of the heterozygous genotype.
T15091 26696-26697 . denotes .
T15090 26687-26696 NN denotes offspring
T15089 26674-26686 JJ denotes heterozygous
T15088 26671-26673 IN denotes of
T15087 26651-26659 VBN denotes expected
T15086 26646-26650 IN denotes than
T15085 26638-26645 JJR denotes greater
T15084 26660-26670 NN denotes proportion
T15083 26636-26637 DT denotes a
T15082 26631-26635 IN denotes with
T15081 26623-26630 NN denotes progeny
T15080 26609-26613 RB denotes also
T15079 26591-26603 JJ denotes heterozygous
T15078 26587-26590 CC denotes and
T15077 26604-26608 NNS denotes mice
T15076 26581-26582 HYPH denotes -
T15075 26582-26586 NN denotes type
T15074 26577-26581 JJ denotes wild
T15073 26569-26576 IN denotes between
T15072 26614-26622 VBD denotes produced
T15071 26561-26568 NNS denotes Crosses
T15070 26560-26697 sentence denotes Crosses between wild-type and heterozygous mice also produced progeny with a greater than expected proportion of heterozygous offspring.
T15069 26559-26560 . denotes .
T15068 26553-26559 NN denotes result
T15067 26548-26552 DT denotes this
T15066 26544-26547 IN denotes for
T15065 26524-26531 JJ denotes obvious
T15064 26532-26543 NN denotes explanation
T15063 26521-26523 DT denotes no
T15062 26512-26517 EX denotes there
T15061 26510-26512 , denotes ,
T15060 26503-26510 JJ denotes present
T15059 26518-26520 VBZ denotes is
T15058 26500-26502 IN denotes At
T15057 26499-26560 sentence denotes At present, there is no obvious explanation for this result.
T15056 26498-26499 . denotes .
T15055 26489-26498 NNS denotes weanlings
T15054 26486-26488 IN denotes of
T15053 26484-26485 -RRB- denotes )
T15052 26479-26483 CD denotes 88.4
T15051 26483-26484 NN denotes %
T15050 26478-26479 -LRB- denotes (
T15049 26476-26477 SYM denotes -
T15048 26475-26476 HYPH denotes /
T15047 26474-26475 SYM denotes +
T15046 26469-26474 NN denotes Capn2
T15045 26466-26468 IN denotes to
T15044 26464-26465 -RRB- denotes )
T15043 26459-26463 CD denotes 11.6
T15042 26463-26464 NN denotes %
T15041 26458-26459 -LRB- denotes (
T15040 26456-26457 SYM denotes +
T15039 26455-26456 HYPH denotes /
T15038 26454-26455 SYM denotes +
T15037 26449-26454 NN denotes Capn2
T15036 26446-26448 IN denotes of
T15035 26426-26439 JJ denotes non-Mendelian
T15034 26419-26425 RB denotes highly
T15033 26440-26445 NN denotes ratio
T15032 26415-26418 DT denotes the
T15031 26397-26410 NN denotes interbreeding
T15030 26384-26396 JJ denotes heterozygous
T15029 26379-26383 IN denotes from
T15028 26371-26378 NN denotes progeny
T15027 26368-26370 IN denotes of
T15026 26357-26367 NN denotes genotyping
T15025 26353-26356 DT denotes the
T15024 26346-26352 IN denotes during
T15023 26340-26345 VBD denotes arose
T15022 26334-26339 WDT denotes which
T15021 26411-26414 VBD denotes was
T15020 26324-26333 NN denotes curiosity
T15019 26320-26323 CD denotes One
T15018 26319-26499 sentence denotes One curiosity which arose during the genotyping of progeny from heterozygous interbreeding was the highly non-Mendelian ratio of Capn2+/+ (11.6%) to Capn2+/- (88.4%) of weanlings.
T15017 26318-26319 . denotes .
T15016 26310-26318 NNS denotes attempts
T15015 26304-26309 DT denotes these
T15014 26291-26294 RB denotes far
T15013 26286-26290 RB denotes thus
T15012 26282-26285 VBZ denotes has
T15011 26265-26271 NN denotes rescue
T15010 26272-26281 NN denotes transgene
T15009 26261-26264 DT denotes the
T15008 26258-26260 IN denotes of
T15007 26247-26257 NN denotes expression
T15006 26240-26246 JJ denotes stable
T15005 26230-26239 VBG denotes achieving
T15004 26227-26229 IN denotes in
T15003 26216-26226 NN denotes difficulty
T15002 26214-26216 , denotes ,
T15001 26295-26303 VBN denotes hampered
T15000 26207-26214 RB denotes However
T14999 26206-26319 sentence denotes However, difficulty in achieving stable expression of the rescue transgene has thus far hampered these attempts.
T14998 26205-26206 . denotes .
T14997 26198-26200 VBZ denotes is
T14996 26185-26186 HYPH denotes -
T14995 26186-26190 NN denotes type
T14994 26181-26185 JJ denotes wild
T14993 26170-26180 JJ denotes endogenous
T14992 26160-26169 VBG denotes remaining
T14991 26191-26197 NN denotes allele
T14990 26156-26159 DT denotes the
T14989 26201-26205 VBN denotes lost
T14988 26149-26155 IN denotes before
T14987 26140-26148 NN denotes activity
T14986 26131-26132 HYPH denotes -
T14985 26132-26139 NN denotes calpain
T14984 26130-26131 NN denotes m
T14983 26110-26122 RB denotes preemptively
T14982 26123-26129 VB denotes rescue
T14981 26107-26109 TO denotes to
T14980 26101-26106 NN denotes order
T15551 27881-27890 NN denotes targeting
T15550 27876-27880 NN denotes gene
T15549 27864-27875 JJ denotes conditional
T15548 27891-27899 NN denotes strategy
T15547 27862-27863 DT denotes a
T15546 27856-27861 VBG denotes using
T15545 27843-27845 VB denotes be
T15544 27846-27855 VBN denotes addressed
T15543 27840-27842 TO denotes to
T15542 27835-27839 VB denotes have
T15541 27830-27834 MD denotes will
T15540 27826-27829 CC denotes and
T15539 27812-27814 VB denotes be
T15538 27815-27825 VBN denotes elucidated
T15537 27809-27811 TO denotes to
T15536 27797-27801 NNS denotes mice
T15535 27791-27796 JJ denotes adult
T15534 27788-27790 IN denotes in
T15533 27784-27787 CC denotes and
T15532 27770-27783 NN denotes embryogenesis
T15531 27752-27769 JJ denotes post-implantation
T15530 27745-27751 IN denotes during
T15529 27736-27737 HYPH denotes -
T15528 27737-27744 NN denotes calpain
T15527 27735-27736 NN denotes m
T15526 27732-27734 IN denotes of
T15525 27802-27808 VBP denotes remain
T15524 27722-27731 NNS denotes functions
T15523 27718-27721 DT denotes The
T15522 27717-27900 sentence denotes The functions of m-calpain during post-implantation embryogenesis and in adult mice remain to be elucidated and will have to be addressed using a conditional gene targeting strategy.
T15521 27716-27717 . denotes .
T15520 27712-27716 FW denotes vivo
T15519 27709-27711 FW denotes in
T15518 27694-27702 JJ denotes distinct
T15517 27703-27708 NNS denotes roles
T15516 27689-27693 DT denotes some
T15515 27676-27683 RB denotes clearly
T15514 27663-27666 CD denotes two
T15513 27667-27675 NNS denotes isoforms
T15512 27659-27662 DT denotes the
T15511 27684-27688 VBP denotes have
T15510 27654-27658 IN denotes that
T15509 27640-27653 VBG denotes demonstrating
T15508 27638-27640 , denotes ,
T15507 27630-27631 HYPH denotes -
T15506 27629-27630 NN denotes m
T15505 27625-27628 CC denotes and
T15504 27623-27624 HYPH denotes -
T15503 27631-27638 NN denotes calpain
T15502 27622-27623 NN denotes μ
T15501 27619-27621 IN denotes of
T15500 27600-27605 FW denotes vitro
T15499 27597-27599 FW denotes in
T15498 27588-27596 JJ denotes apparent
T15497 27606-27618 NNS denotes similarities
T15496 27584-27587 DT denotes the
T15495 27576-27583 IN denotes despite
T15494 27567-27568 HYPH denotes -
T15493 27568-27575 NN denotes calpain
T15492 27566-27567 NN denotes μ
T15491 27563-27565 IN denotes by
T15490 27559-27562 RP denotes out
T15489 27548-27550 VB denotes be
T15488 27544-27547 RB denotes not
T15487 27541-27544 MD denotes can
T15486 27532-27540 NN denotes function
T15485 27527-27531 DT denotes this
T15484 27525-27527 , denotes ,
T15483 27551-27558 VBN denotes carried
T15482 27514-27525 RB denotes Furthermore
T15481 27513-27717 sentence denotes Furthermore, this function cannot be carried out by μ-calpain despite the apparent in vitro similarities of μ- and m-calpain, demonstrating that the two isoforms clearly have some distinct roles in vivo.
T15480 27512-27513 . denotes .
T15479 27501-27512 NN denotes development
T15478 27484-27500 JJ denotes pre-implantation
T15477 27481-27483 IN denotes to
T15476 27473-27480 VBN denotes related
T15475 27464-27472 RB denotes possibly
T15474 27462-27464 , denotes ,
T15473 27449-27462 NN denotes embryogenesis
T15472 27442-27448 JJ denotes murine
T15471 27439-27441 IN denotes in
T15470 27420-27433 JJ denotes indispensable
T15469 27434-27438 NN denotes role
T15468 27417-27419 DT denotes an
T15467 27402-27403 HYPH denotes -
T15466 27403-27410 NN denotes calpain
T15465 27401-27402 NN denotes m
T15464 27411-27416 VBZ denotes plays
T15463 27396-27400 IN denotes that
T15462 27381-27384 VBD denotes was
T15461 27378-27380 PRP denotes it
T15460 27376-27378 , denotes ,
T15459 27385-27395 VBN denotes determined
T15458 27369-27376 RB denotes Firstly
T15457 27368-27513 sentence denotes Firstly, it was determined that m-calpain plays an indispensable role in murine embryogenesis, possibly related to pre-implantation development.
T15456 27367-27368 . denotes .
T15455 27359-27360 HYPH denotes -
T15454 27358-27359 NN denotes m
T15453 27354-27357 CC denotes and
T15452 27352-27353 HYPH denotes -
T15451 27360-27367 NN denotes calpain
T15450 27351-27352 NN denotes μ
T15449 27349-27351 , denotes ,
T14979 26098-26100 IN denotes in
T14978 26087-26097 NN denotes conversion
T14977 26082-26086 NN denotes gene
T14976 26079-26081 IN denotes to
T14975 26073-26078 JJ denotes prior
T14974 26056-26062 NN denotes rescue
T14973 26051-26055 NN denotes cDNA
T14972 26045-26050 NN denotes Capn2
T14971 26063-26072 NN denotes transgene
T14970 26043-26044 DT denotes a
T14969 26038-26042 IN denotes from
T14968 26028-26029 NN denotes k
T14967 26025-26027 CD denotes 80
T14966 26024-26025 HYPH denotes -
T14965 26023-26024 NN denotes m
T14964 26030-26037 NN denotes subunit
T14963 26019-26022 DT denotes the
T14962 26011-26018 VB denotes express
T14961 26008-26010 TO denotes to
T14960 26004-26007 RB denotes way
T14959 25998-26003 RB denotes under
T14958 25994-25997 VBP denotes are
T14957 25986-25993 NNS denotes Efforts
T14956 25985-26206 sentence denotes Efforts are under way to express the m-80 k subunit from a Capn2 cDNA rescue transgene prior to gene conversion in order to preemptively rescue m-calpain activity before the remaining endogenous wild-type allele is lost.
T14955 25984-25985 . denotes .
T14954 25979-25984 NNS denotes cells
T14953 25976-25978 NN denotes ES
T14952 25973-25975 IN denotes of
T14951 25963-25972 NN denotes selection
T14950 25956-25962 JJ denotes clonal
T14949 25953-25955 CC denotes or
T14948 25943-25952 NN denotes viability
T14947 25938-25942 NN denotes cell
T14946 25918-25925 RB denotes somehow
T14945 25905-25911 NN denotes mutant
T14885 25592-25740 sentence denotes Furthermore, calpain-independent mechanisms for protecting cell viability might exist in these cell lines that are absent in early embryonic cells.
T14884 25591-25592 . denotes .
T14883 25586-25591 NNS denotes cells
T14882 25582-25585 DT denotes the
T14881 25579-25581 IN denotes of
T14880 25569-25578 NN denotes viability
T14879 25565-25568 DT denotes the
T14878 25553-25564 VBG denotes maintaining
T14877 25549-25552 IN denotes for
T14876 25538-25548 JJ denotes sufficient
T14875 25535-25537 VBZ denotes is
T14874 25532-25534 PRP denotes it
T14873 25528-25531 CC denotes and
T14872 25526-25528 , denotes ,
T14871 25514-25519 NN denotes Capn4
T14870 25507-25513 NN denotes mutant
T14869 25520-25526 NN denotes allele
T14868 25502-25506 DT denotes this
T14867 25497-25501 IN denotes from
T14866 25485-25487 VBZ denotes is
T14865 25483-25485 , denotes ,
T14864 25476-25483 NNS denotes methods
T14863 25470-25475 DT denotes these
T14862 25467-25469 IN denotes by
T14861 25456-25466 JJ denotes detectable
T14860 25449-25455 NNS denotes levels
T14859 25441-25448 IN denotes beneath
T14858 25439-25441 , denotes ,
T14857 25431-25439 NN denotes activity
T14856 25423-25430 NN denotes calpain
T14855 25420-25422 IN denotes of
T14854 25407-25412 NN denotes trace
T14853 25413-25419 NN denotes amount
T14852 25405-25406 DT denotes a
T14851 25488-25496 VBN denotes retained
T14850 25400-25404 IN denotes that
T14849 25398-25400 , denotes ,
T14848 25391-25398 RBR denotes earlier
T14847 25381-25390 VBN denotes discussed
T14846 25378-25380 IN denotes as
T14845 25376-25378 , denotes ,
T14844 25365-25376 JJ denotes conceivable
T14843 25362-25364 VBZ denotes is
T14842 25359-25361 PRP denotes It
T14841 25358-25592 sentence denotes It is conceivable, as discussed earlier, that a trace amount of calpain activity, beneath levels detectable by these methods, is retained from this mutant Capn4 allele, and it is sufficient for maintaining the viability of the cells.
T14840 25357-25358 . denotes .
T14839 25339-25348 NN denotes breakdown
T14838 25330-25338 NN denotes spectrin
T14837 25349-25357 NNS denotes products
T14836 25315-25329 JJ denotes characteristic
T14835 25312-25314 IN denotes of
T14834 25301-25311 NN denotes appearance
T14833 25297-25300 DT denotes the
T14832 25294-25296 IN denotes by
T14831 25291-25293 CC denotes or
T14830 25280-25290 NN denotes zymography
T14829 25273-25279 NN denotes casein
T14828 25270-25272 IN denotes by
T14827 25261-25269 VBN denotes assessed
T14826 25258-25260 IN denotes as
T14825 25256-25258 , denotes ,
T14824 25248-25256 NN denotes activity
T14823 25240-25247 NN denotes calpain
T14822 25237-25239 IN denotes of
T14821 25223-25231 JJ denotes apparent
T14820 25232-25236 NN denotes lack
T14819 25220-25222 DT denotes an
T14818 25212-25219 IN denotes despite
T14817 25204-25211 NN denotes culture
T14816 25201-25203 IN denotes in
T14815 25187-25189 VB denotes be
T14814 25183-25186 MD denotes can
T14813 25164-25175 JJ denotes hypomorphic
T14812 25152-25163 JJ denotes presumptive
T14811 25176-25182 NN denotes allele
T14810 25148-25151 DT denotes the
T14809 25143-25147 IN denotes from
T14808 25133-25136 NN denotes MEF
T14807 25129-25132 CC denotes and
T14806 25126-25128 NN denotes ES
T14805 25124-25125 SYM denotes -
T14804 25123-25124 HYPH denotes /
T14803 25122-25123 SYM denotes -
T14802 25137-25142 NNS denotes cells
T14801 25117-25122 NN denotes Capn4
T14800 25190-25200 VBN denotes maintained
T14799 25112-25116 IN denotes that
T14798 25100-25102 VB denotes be
T14797 25095-25099 RB denotes also
T14796 25088-25094 MD denotes should
T14795 25103-25111 VBN denotes stressed
T14794 25085-25087 PRP denotes It
T14793 25084-25358 sentence denotes It should also be stressed that Capn4-/- ES and MEF cells from the presumptive hypomorphic allele can be maintained in culture despite an apparent lack of calpain activity, as assessed by casein zymography or by the appearance of characteristic spectrin breakdown products.
T14792 25083-25084 . denotes .
T14791 25062-25071 JJ denotes embryonic
T14790 25072-25083 NN denotes development
T14789 25056-25061 JJ denotes early
T14788 25053-25055 IN denotes on
T14787 25045-25052 NNS denotes effects
T14786 25038-25044 JJ denotes lethal
T14785 25028-25032 RB denotes both
T14784 25022-25027 MD denotes would
T14783 24999-25009 NN denotes chromosome
T14782 25010-25021 NN denotes segregation
T14781 24989-24998 JJ denotes defective
T14780 24986-24988 CC denotes or
T14779 25033-25037 VB denotes have
T14778 24975-24985 NN denotes Polyspermy
T14777 24974-25084 sentence denotes Polyspermy or defective chromosome segregation would both have lethal effects on early embryonic development.
T14776 24973-24974 . denotes .
T14775 24972-24973 -RRB- denotes ]
T14774 24970-24972 CD denotes 45
T14773 24969-24970 -LRB- denotes [
T14772 24957-24968 NN denotes segregation
T14771 24946-24956 NN denotes chromosome
T14770 24943-24945 IN denotes in
T14769 24931-24933 VB denotes be
T14768 24925-24930 MD denotes could
T14767 24922-24924 PRP denotes it
T14766 24934-24942 VBN denotes involved
T14765 24917-24921 VBD denotes were
T14764 24915-24917 , denotes ,
T14763 24902-24915 NN denotes fertilization
T14762 24896-24901 IN denotes after
T14761 24880-24887 JJ denotes meiotic
T14760 24873-24879 NN denotes oocyte
T14759 24888-24895 NN denotes spindle
T14758 24869-24872 DT denotes the
T14757 24866-24868 IN denotes to
T14756 24849-24853 RB denotes also
T14755 24854-24865 VBD denotes relocalized
T14754 24840-24841 HYPH denotes -
T14753 24841-24848 NN denotes calpain
T14752 24839-24840 NN denotes m
T14751 24838-24974 sentence denotes m-calpain also relocalized to the oocyte meiotic spindle after fertilization, were it could be involved in chromosome segregation [45].
T14750 24837-24838 . denotes .
T14749 24836-24837 -RRB- denotes ]
T14748 24834-24836 CD denotes 45
T14747 24833-24834 -LRB- denotes [
T14746 24822-24832 NN denotes polyspermy
T14745 24814-24821 VB denotes prevent
T14744 24811-24813 TO denotes to
T14743 24802-24810 VBN denotes required
T14742 24785-24792 NN denotes granule
T14741 24793-24801 NNS denotes contents
T14740 24776-24784 JJ denotes cortical
T14739 24773-24775 IN denotes of
T14738 24765-24772 NN denotes release
T14737 24761-24764 DT denotes the
T14736 24758-24760 IN denotes in
T14735 24740-24745 MD denotes might
T14734 24737-24739 PRP denotes it
T14733 24746-24757 VB denotes participate
T14732 24731-24736 WRB denotes where
T14731 24723-24730 NNS denotes oocytes
T14730 24720-24722 IN denotes in
T14729 24702-24710 JJ denotes cortical
T14728 24711-24719 NN denotes membrane
T14727 24698-24701 DT denotes the
T14726 24695-24697 IN denotes to
T14725 24686-24687 HYPH denotes -
T14724 24687-24694 NN denotes calpain
T14723 24685-24686 NN denotes m
T14722 24682-24684 IN denotes of
T14721 24668-24681 NN denotes translocation
T14720 24666-24667 DT denotes a
T14719 24661-24665 IN denotes with
T14718 24650-24660 VBD denotes correlated
T14717 24644-24649 WDT denotes which
T14716 24642-24643 -RRB- denotes ]
T14715 24640-24642 CD denotes 47
T14714 24639-24640 -LRB- denotes [
T14713 24621-24629 NN denotes acrosome
T14712 24630-24638 NN denotes reaction
T14711 24617-24620 DT denotes the
T14710 24614-24616 IN denotes in
T14709 24599-24602 VBD denotes was
T14708 24590-24591 HYPH denotes -
T14707 24591-24598 NN denotes calpain
T14706 24589-24590 NN denotes m
T14705 24587-24588 . denotes .
T16087 28243-28245 PRP denotes It
T16086 28242-28405 sentence denotes It was found to contain 2.8-kb of sequence from position 247 of the coding sequence to the stop codon at position 2,101, including the 3'-UTR to the polyA signal.
T16085 28241-28242 . denotes .
T16084 28240-28241 -RRB- denotes )
T16083 28234-28236 , denotes ,
T16082 28224-28234 NNP denotes Consortium
T16081 28218-28223 NNP denotes Image
T16080 28216-28218 , denotes ,
T16079 28210-28216 CD denotes 606689
T16078 28209-28210 : denotes :
T16077 28204-28209 NNP denotes Image
T16076 28202-28204 , denotes ,
T16075 28196-28202 CD denotes 807416
T16074 28194-28196 , denotes ,
T16073 28192-28194 NN denotes Id
T16072 28186-28191 NN denotes dbEST
T16071 28236-28240 NNP denotes LLNL
T16070 28185-28186 -LRB- denotes (
T16069 28171-28174 VBD denotes was
T16068 28158-28160 CD denotes 80
T16067 28157-28158 HYPH denotes -
T16066 28161-28162 NN denotes k
T16065 28156-28157 NN denotes m
T16064 28150-28155 NN denotes mouse
T16063 28163-28170 NN denotes subunit
T16062 28146-28149 DT denotes the
T16061 28143-28145 IN denotes of
T16060 28135-28142 NN denotes portion
T16059 28133-28134 DT denotes a
T16058 28124-28132 VBG denotes encoding
T16057 28175-28184 VBN denotes purchased
T16056 28113-28117 NN denotes cDNA
T16055 28118-28123 NN denotes clone
T16054 28111-28112 DT denotes A
T16053 28110-28242 sentence denotes A cDNA clone encoding a portion of the mouse m-80 k subunit was purchased (dbEST Id, 807416, Image:606689, Image Consortium, LLNL).
T16052 28109-28110 . denotes .
T16051 28108-28109 CD denotes 1
T16050 28097-28107 NN denotes chromosome
T16049 28094-28096 IN denotes on
T16048 28090-28091 HYPH denotes -
T16047 28091-28093 NN denotes kb
T16046 28088-28090 CD denotes 50
T16045 28083-28087 IN denotes over
T16044 28073-28082 VBG denotes extending
T16043 28067-28072 NNS denotes exons
T16042 28064-28066 CD denotes 21
T16041 28061-28063 IN denotes of
T16040 28052-28060 VBZ denotes consists
T16039 28048-28051 CC denotes and
T16038 28046-28047 -RRB- denotes )
T16037 28042-28044 CD denotes 80
T16036 28041-28042 HYPH denotes -
T16035 28040-28041 NN denotes m
T16034 28045-28046 NN denotes k
T16033 28039-28040 -LRB- denotes (
T16032 28025-28030 JJ denotes large
T16031 28016-28017 HYPH denotes -
T16030 28017-28024 NN denotes calpain
T16029 28015-28016 NN denotes m
T16028 28031-28038 NN denotes subunit
T16027 28011-28014 DT denotes the
T16026 28008-28010 IN denotes of
T16025 27996-28001 NN denotes amino
T16024 27992-27995 CD denotes 700
T16023 28002-28007 NNS denotes acids
T16022 27988-27991 DT denotes the
T16021 27980-27987 VBZ denotes encodes
T16020 27969-27974 NN denotes Capn2
T16019 27963-27968 NN denotes mouse
T16018 27975-27979 NN denotes gene
T16017 27959-27962 DT denotes The
T16016 27958-28110 sentence denotes The mouse Capn2 gene encodes the 700 amino acids of the m-calpain large subunit (m-80 k) and consists of 21 exons extending over 50-kb on chromosome 1.
T16015 27947-27952 NN denotes Capn2
T16014 27941-27946 NN denotes mouse
T16013 27953-27958 NN denotes locus
T16012 27937-27940 DT denotes the
T16011 27934-27936 IN denotes of
T16010 27923-27933 NN denotes sequencing
T16009 27919-27922 CC denotes and
T16008 27911-27918 NN denotes Cloning
T15161 27068-27069 DT denotes a
T14704 24586-24587 -RRB- denotes ]
T14703 24584-24586 CD denotes 45
T14702 24583-24584 -LRB- denotes [
T14701 24575-24582 NNS denotes oocytes
T14700 24571-24574 CC denotes and
T14699 24569-24570 -RRB- denotes ]
T14698 24567-24569 CD denotes 47
T14697 24566-24567 -LRB- denotes [
T14696 24560-24565 NN denotes sperm
T14695 24553-24559 NN denotes rodent
T14694 24550-24552 IN denotes in
T14693 24603-24613 VBN denotes implicated
T14692 24536-24540 VBN denotes been
T14691 24527-24535 RB denotes recently
T14690 24522-24526 VBP denotes have
T14689 24541-24549 VBN denotes detected
T14688 24513-24514 HYPH denotes -
T14687 24512-24513 NN denotes m
T14686 24508-24511 CC denotes and
T14685 24506-24507 HYPH denotes -
T14684 24514-24521 NN denotes calpain
T14683 24505-24506 NN denotes μ
T14682 24500-24504 CC denotes Both
T14681 24499-24838 sentence denotes Both μ- and m-calpain have recently been detected in rodent sperm [47] and oocytes [45]. m-calpain was implicated in the acrosome reaction [47] which correlated with a translocation of m-calpain to the cortical membrane in oocytes where it might participate in the release of cortical granule contents required to prevent polyspermy [45].
T14680 24498-24499 . denotes .
T14679 24497-24498 -RRB- denotes ]
T14678 24495-24497 CD denotes 46
T14677 24494-24495 -LRB- denotes [
T14676 24476-24484 NN denotes acrosome
T14675 24485-24493 NN denotes reaction
T14674 24472-24475 DT denotes the
T14673 24463-24471 VBZ denotes triggers
T14672 24457-24462 WDT denotes which
T14671 24437-24448 JJ denotes cytoplasmic
T14670 24449-24456 NN denotes calcium
T14609 24147-24151 VBN denotes been
T14608 24142-24146 RB denotes also
T14607 24138-24141 VBZ denotes has
T14606 24152-24162 VBN denotes associated
T14605 24130-24137 NN denotes Calpain
T14604 24129-24223 sentence denotes Calpain has also been associated with progression through the G1 stage of the cell cycle [6].
T14603 24128-24129 . denotes .
T14602 24127-24128 -RRB- denotes ]
T14601 24126-24127 CD denotes 5
T14600 24125-24126 -LRB- denotes [
T14599 24110-24114 NN denotes cell
T14598 24115-24124 NNS denotes densities
T14597 24106-24109 JJ denotes low
T14596 24101-24105 RB denotes very
T14595 24093-24097 RB denotes only
T14594 24098-24100 IN denotes at
T14593 24089-24092 CC denotes but
T14592 24087-24089 , denotes ,
T14591 24074-24087 NN denotes proliferation
T14590 24069-24073 NN denotes cell
T14589 24059-24068 VB denotes influence
T14588 24056-24058 TO denotes to
T14587 24046-24049 VBD denotes was
T14586 24038-24045 NN denotes calpain
T14585 24036-24038 , denotes ,
T14584 24030-24031 SYM denotes -
T14583 24029-24030 HYPH denotes /
T14582 24028-24029 SYM denotes -
T14581 24032-24036 NNS denotes MEFs
T14580 24023-24028 NN denotes Capn4
T14579 24019-24022 CC denotes and
T14578 24009-24012 NN denotes CHO
T14577 24013-24018 NNS denotes cells
T14576 24004-24008 CC denotes both
T14575 24050-24055 VBN denotes shown
T14574 24001-24003 IN denotes In
T14573 24000-24129 sentence denotes In both CHO cells and Capn4-/- MEFs, calpain was shown to influence cell proliferation, but only at very low cell densities [5].
T14572 23999-24000 . denotes .
T14571 23998-23999 -RRB- denotes ]
T14570 23996-23997 , denotes ,
T14569 23995-23996 CD denotes 5
T14568 23997-23998 CD denotes 6
T14567 23994-23995 -LRB- denotes [
T14566 23984-23993 JJ denotes essential
T14565 23975-23983 RB denotes strictly
T14564 23963-23970 NN denotes calpain
T14563 23971-23974 VBD denotes was
T14562 23958-23962 IN denotes that
T14561 23949-23952 RB denotes not
T14560 23953-23957 VB denotes show
T14559 23945-23948 VBD denotes did
T14558 23941-23944 CC denotes and
T14557 23939-23941 , denotes ,
T14556 23931-23932 HYPH denotes -
T14555 23930-23931 NN denotes m
T14554 23926-23929 CC denotes and
T14553 23924-23925 HYPH denotes -
T14552 23932-23939 NN denotes calpain
T14551 23923-23924 NN denotes μ
T14550 23915-23922 IN denotes between
T14549 23899-23902 RB denotes not
T14548 23895-23898 VBD denotes did
T14547 23884-23894 RB denotes frequently
T14546 23870-23878 VBN denotes reported
T14545 23879-23883 NN denotes work
T14544 23866-23869 DT denotes the
T14543 23903-23914 VB denotes distinguish
T14542 23857-23865 IN denotes although
T14541 23855-23857 , denotes ,
T14540 23845-23849 NN denotes cell
T14539 23850-23855 NNS denotes lines
T14538 23839-23844 JJ denotes other
T14537 23835-23838 CC denotes and
T14536 23828-23829 SYM denotes -
T14535 23827-23828 HYPH denotes /
T14534 23826-23827 SYM denotes -
T14533 23830-23834 NNS denotes MEFs
T14532 23821-23826 NN denotes Capn4
T14531 23818-23820 IN denotes in
T14530 23807-23811 VBN denotes been
T14529 23803-23806 VBZ denotes has
T14528 23793-23802 NN denotes viability
T14527 23788-23792 NN denotes cell
T14526 23784-23787 CC denotes and
T14525 23776-23783 NN denotes calpain
T14524 23768-23775 IN denotes between
T14523 23812-23817 VBN denotes noted
T14522 23756-23767 NN denotes association
T14521 23752-23755 DT denotes The
T14520 23751-24000 sentence denotes The association between calpain and cell viability has been noted in Capn4-/- MEFs and other cell lines, although the reported work frequently did not distinguish between μ- and m-calpain, and did not show that calpain was strictly essential [5,6].
T14519 23750-23751 . denotes .
T14518 23729-23738 JJ denotes embryonic
T14517 23739-23750 NN denotes development
T14516 23722-23728 VBN denotes failed
T14515 23719-23721 IN denotes to
T14514 23702-23707 MD denotes could
T14513 23700-23702 , denotes ,
T14512 23699-23700 -RRB- denotes ]
T14511 23698-23699 CD denotes 9
T14510 23697-23698 -LRB- denotes [
T14509 23689-23690 SYM denotes -
T14508 23688-23689 HYPH denotes /
T14507 23687-23688 SYM denotes -
T14506 23691-23696 NNS denotes cells
T14505 23682-23687 NN denotes Capn4
T14504 23679-23681 IN denotes in
T14503 23670-23678 VBN denotes reported
T14502 23668-23670 , denotes ,
T14501 23659-23668 NN denotes migration
T14500 23656-23658 IN denotes in
T14499 23708-23718 VB denotes contribute
T14498 23648-23655 NNS denotes Defects
T14497 23647-23751 sentence denotes Defects in migration, reported in Capn4-/- cells [9], could contribute to failed embryonic development.
T14496 23646-23647 . denotes .
T14495 23645-23646 -RRB- denotes ]
T14494 23643-23645 CD denotes 45
T14493 23642-23643 -LRB- denotes [
T14492 23634-23641 NN denotes meiosis
T14491 23627-23633 IN denotes during
T14490 23619-23623 RB denotes well
T14489 23624-23626 IN denotes as
T14488 23616-23618 RB denotes as
T14487 23614-23615 -RRB- denotes ]
T14486 23612-23614 CD denotes 44
T14485 23611-23612 -LRB- denotes [
T14484 23603-23610 NN denotes mitosis
T14483 23596-23602 IN denotes during
T14482 23584-23595 NN denotes segregation
T14481 23573-23583 NN denotes chromosome
T14480 23570-23572 IN denotes in
T14479 23558-23569 NN denotes involvement
T14478 23554-23557 PRP$ denotes its
T14477 23551-23553 IN denotes of
T14476 23543-23550 NNS denotes reports
T14475 23533-23542 VBG denotes including
T14474 23531-23533 , denotes ,
T14473 23518-23531 NNS denotes circumstances
T14472 23510-23517 JJ denotes certain
T14471 23507-23509 IN denotes in
T14470 23493-23506 NN denotes proliferation
T14469 23488-23492 NN denotes cell
T14468 23485-23487 IN denotes in
T14467 23473-23484 NN denotes involvement
T14466 23464-23465 HYPH denotes -
T14465 23465-23472 NN denotes calpain
T14464 23463-23464 NN denotes m
T14463 23460-23462 IN denotes of
T14462 23452-23459 NNS denotes reports
T14461 23444-23451 JJ denotes several
T14460 23434-23438 VBP denotes have
T14459 23439-23443 VBN denotes been
T14458 23428-23433 EX denotes There
T14457 23427-23647 sentence denotes There have been several reports of m-calpain involvement in cell proliferation in certain circumstances, including reports of its involvement in chromosome segregation during mitosis [44] as well as during meiosis [45].
T14456 23426-23427 . denotes .
T14455 23419-23426 JJ denotes unclear
T14454 23413-23418 RB denotes still
T14453 23400-23401 HYPH denotes -
T14452 23401-23408 NN denotes calpain
T14451 23399-23400 NN denotes m
T14450 23396-23398 IN denotes of
T14449 23388-23395 NN denotes absence
T14448 23384-23387 DT denotes the
T14447 23381-23383 IN denotes in
T14446 23371-23380 NN denotes lethality
T14445 23367-23370 IN denotes for
T14444 23409-23412 VBP denotes are
T14443 23347-23358 JJ denotes mechanistic
T14442 23359-23366 NNS denotes reasons
T14441 23343-23346 DT denotes The
T14440 23342-23427 sentence denotes The mechanistic reasons for lethality in the absence of m-calpain are still unclear.
T14439 23341-23342 . denotes .
T14438 23327-23331 NN denotes cell
T14437 23332-23341 NNS denotes divisions
T14436 23324-23325 SYM denotes –
T14435 23325-23326 CD denotes 3
T14434 23323-23324 CD denotes 2
T14433 23315-23322 IN denotes through
T14432 23307-23314 NN denotes protein
T14431 23304-23306 CC denotes or
T14430 23303-23304 HYPH denotes /
T14429 23300-23303 CC denotes and
T14944 25894-25904 JJ denotes homozygous
T14943 25912-25917 NN denotes state
T14942 25890-25893 DT denotes the
T14941 25926-25937 VBZ denotes compromises
T14940 25885-25889 IN denotes that
T14939 25871-25875 NN denotes G418
T14938 25868-25870 IN denotes of
T14937 25853-25867 NNS denotes concentrations
T14936 25848-25852 JJ denotes high
T14935 25845-25847 IN denotes in
T14934 25838-25844 NNS denotes clones
T14933 25835-25837 IN denotes of
T14932 25825-25834 NN denotes selection
T14931 25822-25824 IN denotes by
T14930 25816-25821 NNS denotes cells
T14929 25813-25815 NN denotes ES
T14928 25811-25812 SYM denotes -
T14927 25810-25811 HYPH denotes /
T14926 25809-25810 SYM denotes -
T14925 25804-25809 NN denotes Capn2
T14924 25801-25803 IN denotes to
T14923 25799-25800 SYM denotes -
T14922 25798-25799 HYPH denotes /
T14921 25797-25798 SYM denotes +
T14920 25792-25797 NN denotes Capn2
T14919 25789-25791 IN denotes of
T14918 25778-25788 NN denotes conversion
T14917 25773-25777 NN denotes gene
T14916 25765-25772 VB denotes achieve
T14915 25762-25764 TO denotes to
T14914 25876-25884 VBZ denotes suggests
T14913 25745-25753 VBN denotes repeated
T14912 25754-25761 NN denotes failure
T14911 25741-25744 DT denotes The
T14910 25740-25985 sentence denotes The repeated failure to achieve gene conversion of Capn2+/- to Capn2-/- ES cells by selection of clones in high concentrations of G418 suggests that the homozygous mutant state somehow compromises cell viability or clonal selection of ES cells.
T14909 25739-25740 . denotes .
T14908 25724-25733 JJ denotes embryonic
T14907 25734-25739 NNS denotes cells
T14906 25718-25723 JJ denotes early
T14905 25715-25717 IN denotes in
T14904 25708-25714 JJ denotes absent
T14903 25704-25707 VBP denotes are
T14902 25699-25703 WDT denotes that
T14901 25688-25692 NN denotes cell
T14900 25693-25698 NNS denotes lines
T14899 25682-25687 DT denotes these
T14898 25679-25681 IN denotes in
T14897 25667-25672 MD denotes might
T14896 25657-25666 NN denotes viability
T14895 25652-25656 NN denotes cell
T14894 25641-25651 VBG denotes protecting
T14893 25637-25640 IN denotes for
T14892 25626-25636 NNS denotes mechanisms
T14891 25613-25614 HYPH denotes -
T14890 25614-25625 JJ denotes independent
T14889 25606-25613 NN denotes calpain
T14888 25604-25606 , denotes ,
T14887 25673-25678 VB denotes exist
T14886 25593-25604 RB denotes Furthermore
T14428 23295-23299 NN denotes mRNA
T14427 23286-23287 HYPH denotes -
T14426 23285-23286 NN denotes m
T14425 23276-23284 JJ denotes maternal
T14424 23287-23294 NN denotes calpain
T14423 23271-23275 DT denotes some
T14422 23268-23270 IN denotes of
T14421 23256-23267 NN denotes persistence
T14420 23252-23255 DT denotes the
T14419 23249-23251 IN denotes to
T14418 23245-23248 IN denotes due
T14417 23238-23244 RB denotes likely
T14416 23233-23237 RBS denotes most
T14415 23218-23219 HYPH denotes -
T14414 23219-23223 NN denotes cell
T14413 23217-23218 CD denotes 8
T14412 23224-23229 NN denotes stage
T14411 23213-23216 DT denotes the
T14410 23210-23212 IN denotes to
T14409 23200-23201 SYM denotes -
T14408 23199-23200 HYPH denotes /
T14407 23198-23199 SYM denotes -
T14406 23193-23198 NN denotes Capn2
T14405 23189-23192 JJ denotes few
T14404 23202-23209 NNS denotes embryos
T14403 23187-23188 DT denotes a
T14402 23184-23186 IN denotes of
T14401 23175-23183 NN denotes survival
T14400 23171-23174 DT denotes the
T14399 23169-23171 , denotes ,
T14398 23160-23169 NN denotes viability
T14397 23155-23159 NN denotes cell
T14396 23152-23154 IN denotes of
T14395 23145-23151 NN denotes aspect
T14394 23140-23144 DT denotes some
T14376 23068-23072 IN denotes into
T14375 23063-23067 VBZ denotes fits
T14374 23048-23053 NN denotes Capn2
T14373 23054-23062 NN denotes knockout
T14372 23044-23047 DT denotes The
T14371 23043-23093 sentence denotes The Capn2 knockout fits into the latter category.
T14370 23042-23043 . denotes .
T14369 23031-23033 VB denotes be
T14368 23027-23030 RB denotes not
T14367 23021-23026 MD denotes could
T14366 23034-23042 VBN denotes isolated
T14365 23012-23014 NN denotes ES
T14364 23005-23011 NN denotes mutant
T14363 23015-23020 NNS denotes cells
T14362 22994-23004 JJ denotes homozygous
T14361 22990-22993 CC denotes and
T14360 22976-22980 RB denotes ever
T14359 22972-22975 VBP denotes are
T14358 22959-22963 JJ denotes null
T14357 22955-22958 JJ denotes few
T14356 22953-22954 DT denotes a
T14355 22964-22971 NNS denotes embryos
T14354 22948-22952 RB denotes only
T14353 22946-22948 , denotes ,
T14352 22937-22946 NNS denotes instances
T14351 22931-22936 DT denotes these
T14350 22981-22989 VBN denotes observed
T14349 22928-22930 IN denotes In
T14348 22927-23043 sentence denotes In these instances, only a few null embryos are ever observed and homozygous mutant ES cells could not be isolated.
T14347 22926-22927 . denotes .
T14346 22925-22926 -RRB- denotes ]
T14345 22923-22925 CD denotes 43
T14344 22922-22923 SYM denotes -
T14343 22919-22920 , denotes ,
T14342 22918-22919 CD denotes 3
T14341 22920-22922 CD denotes 39
T14340 22917-22918 -LRB- denotes [
T14339 22904-22909 JJ denotes early
T14338 22910-22916 NN denotes embryo
T14337 22900-22903 DT denotes the
T14336 22897-22899 IN denotes in
T14335 22891-22896 NNS denotes cells
T14334 22888-22890 IN denotes of
T14333 22878-22887 NN denotes viability
T14332 22874-22877 DT denotes the
T14331 22866-22873 RB denotes fatally
T14330 22855-22865 VB denotes compromise
T14329 22852-22854 TO denotes to
T14328 22841-22843 VBZ denotes is
T14327 22825-22829 NN denotes gene
T14326 22830-22840 NN denotes disruption
T14325 22821-22824 DT denotes the
T14324 22819-22821 , denotes ,
T14323 22812-22819 RB denotes however
T14322 22810-22812 , denotes ,
T14321 22805-22810 NNS denotes cases
T14320 22799-22804 JJ denotes other
T14319 22844-22851 VBN denotes thought
T14318 22796-22798 IN denotes In
T14317 22795-22927 sentence denotes In other cases, however, the gene disruption is thought to compromise fatally the viability of cells in the early embryo [3,39-43].
T14316 22794-22795 . denotes .
T14315 22781-22788 RB denotes usually
T14314 22778-22780 VBZ denotes is
T14313 22768-22777 NNS denotes genotypes
T14312 22758-22767 JJ denotes embryonic
T14311 22755-22757 IN denotes of
T14310 22789-22794 VBN denotes noted
T14309 22732-22741 JJ denotes Mendelian
T14308 22742-22754 NN denotes distribution
T14307 22730-22731 DT denotes a
T14306 22726-22729 CC denotes and
T14305 22724-22726 , denotes ,
T14304 22712-22718 NN denotes morula
T14303 22719-22724 NN denotes stage
T14302 22708-22711 DT denotes the
T14301 22701-22707 IN denotes beyond
T14300 22687-22692 RB denotes often
T14299 22682-22686 NN denotes type
T14298 22677-22681 DT denotes this
T14297 22674-22676 IN denotes of
T14296 22693-22700 VBP denotes survive
T14295 22666-22673 NNS denotes embryos
T14294 22661-22665 JJ denotes Null
T14293 22660-22795 sentence denotes Null embryos of this type often survive beyond the morula stage, and a Mendelian distribution of embryonic genotypes is usually noted.
T14292 22659-22660 . denotes .
T14291 22658-22659 -RRB- denotes ]
T14290 22656-22658 CD denotes 38
T14289 22655-22656 -LRB- denotes [
T14288 22639-22654 RB denotes morphologically
T14287 22627-22629 VB denotes be
T14286 22621-22626 RB denotes often
T14285 22617-22620 MD denotes can
T14284 22630-22638 VBN denotes observed
T14283 22611-22616 WDT denotes which
T14282 22587-22602 NN denotes differentiation
T14281 22577-22586 JJ denotes embryonic
T14280 22603-22610 NN denotes program
T14279 22573-22576 DT denotes the
T14278 22570-22572 IN denotes in
T14277 22557-22560 VBP denotes are
T14276 22549-22556 NNS denotes defects
T14275 22547-22549 , denotes ,
T14274 22542-22547 NNS denotes cases
T14273 22537-22541 DT denotes some
T14272 22561-22569 VBN denotes incurred
T14271 22534-22536 IN denotes In
T14270 22533-22660 sentence denotes In some cases, defects are incurred in the embryonic differentiation program which can often be observed morphologically [38].
T14269 22532-22533 . denotes .
T14268 22518-22525 JJ denotes general
T14267 22526-22532 NNS denotes causes
T14266 22514-22517 CD denotes two
T14265 22511-22513 IN denotes to
T14264 22497-22499 VB denotes be
T14263 22487-22496 RB denotes typically
T14262 22483-22486 MD denotes can
T14261 22473-22482 NNS denotes knockouts
T14260 22468-22472 NN denotes gene
T14259 22463-22467 IN denotes from
T14258 22453-22462 VBG denotes resulting
T14257 22500-22510 VBN denotes attributed
T14256 22443-22452 NN denotes lethality
T14255 22427-22442 NN denotes Preimplantation
T14254 22426-22533 sentence denotes Preimplantation lethality resulting from gene knockouts can typically be attributed to two general causes.
T14253 22425-22426 . denotes .
T14252 22416-22425 VBG denotes revealing
T14251 22409-22415 PRP denotes itself
T14250 22406-22408 IN denotes in
T14249 22401-22402 SYM denotes -
T14248 22400-22401 HYPH denotes /
T14247 22399-22400 SYM denotes -
T14246 22394-22399 NN denotes Capn2
T14245 22391-22393 VB denotes be
T14244 22388-22390 TO denotes to
T14243 22356-22372 JJ denotes pre-implantation
T14242 22346-22355 VBN denotes genotyped
T14241 22333-22345 RB denotes successfully
T14240 22327-22329 IN denotes of
T14239 22323-22326 IN denotes out
T14238 22330-22332 CD denotes 90
T14237 22319-22322 CD denotes two
T14236 22373-22380 NNS denotes embryos
T14235 22314-22318 RB denotes only
T14234 22381-22387 VBD denotes proved
T14233 22309-22313 IN denotes that
T14232 22304-22308 NN denotes fact
T14231 22300-22303 DT denotes the
T14230 22298-22300 , denotes ,
T14229 22403-22405 VBZ denotes is
T14228 22291-22298 RB denotes However
T14227 22290-22426 sentence denotes However, the fact that only two out of 90 successfully genotyped pre-implantation embryos proved to be Capn2-/- is in itself revealing.
T14226 22289-22290 . denotes .
T14225 22268-22281 JJ denotes morphological
T14224 22260-22267 JJ denotes obvious
T14223 22282-22289 NNS denotes defects
T14222 22256-22259 DT denotes any
T14221 22244-22247 RB denotes not
T14220 22240-22243 VBD denotes did
T14219 22228-22229 HYPH denotes -
T14218 22229-22233 NN denotes cell
T14217 22227-22228 CD denotes 8
T14216 22234-22239 NN denotes stage
T14215 22223-22226 DT denotes the
T14214 22220-22222 IN denotes at
T14213 22209-22219 VBN denotes identified
T14212 22248-22255 VB denotes present
T14211 22196-22200 JJ denotes null
T14210 22185-22195 JJ denotes homozygous
T14209 22181-22184 CD denotes two
T14208 22201-22208 NNS denotes embryos
T14207 22177-22180 DT denotes The
T14206 22176-22290 sentence denotes The two homozygous null embryos identified at the 8-cell stage did not present any obvious morphological defects.
T14205 22175-22176 . denotes .
T14204 22161-22165 VBN denotes been
T14203 22157-22160 RB denotes yet
T14202 22153-22156 RB denotes not
T14201 22149-22152 VBZ denotes has
T14200 22139-22140 SYM denotes -
T14199 22138-22139 HYPH denotes /
T14198 22137-22138 SYM denotes -
T14197 22141-22148 NNS denotes embryos
T14196 22132-22137 NN denotes Capn2
T14669 24427-24436 VBN denotes increased
T14668 24424-24426 IN denotes to
T14667 24411-24417 NN denotes oocyte
T14666 24407-24410 DT denotes the
T14665 24404-24406 IN denotes to
T14664 24418-24423 VBZ denotes leads
T14663 24396-24403 NN denotes binding
T14662 24390-24395 NN denotes Sperm
T14661 24389-24499 sentence denotes Sperm binding to the oocyte leads to increased cytoplasmic calcium which triggers the acrosome reaction [46].
T14660 24388-24389 . denotes .
T14659 24387-24388 -RRB- denotes ]
T14658 24386-24387 CD denotes 3
T14657 24385-24386 -LRB- denotes [
T14656 24374-24384 NNS denotes substrates
T14655 24366-24373 NN denotes calpain
T14654 24363-24365 VB denotes be
T14653 24360-24362 TO denotes to
T14652 24348-24351 VBP denotes are
T14651 24340-24347 NN denotes p27kip1
T14650 24336-24339 CC denotes and
T14649 24334-24336 , denotes ,
T14648 24332-24334 NN denotes D1
T14647 24325-24331 NN denotes cyclin
T14646 24323-24325 , denotes ,
T14645 24319-24323 NN denotes p107
T14644 24317-24319 , denotes ,
T14643 24314-24317 NN denotes p53
T14642 24311-24313 IN denotes as
T14641 24306-24310 JJ denotes such
T14640 24304-24306 , denotes ,
T14639 24293-24304 NN denotes progression
T14638 24287-24292 NN denotes cycle
T14637 24282-24286 NN denotes cell
T14636 24279-24281 IN denotes in
T14635 24267-24269 VB denotes be
T14634 24270-24278 VBN denotes involved
T14633 24264-24266 TO denotes to
T14632 24258-24263 VBN denotes known
T14631 24249-24257 NN denotes proteins
T14630 24245-24248 DT denotes the
T14629 24242-24244 IN denotes of
T14628 24237-24241 DT denotes some
T14627 24235-24237 , denotes ,
T14626 24352-24359 VBN denotes reputed
T14625 24224-24235 RB denotes Furthermore
T14624 24223-24389 sentence denotes Furthermore, some of the proteins known to be involved in cell cycle progression, such as p53, p107, cyclin D1, and p27kip1 are reputed to be calpain substrates [3].
T14623 24222-24223 . denotes .
T14622 24221-24222 -RRB- denotes ]
T14621 24220-24221 CD denotes 6
T14620 24219-24220 -LRB- denotes [
T14619 24208-24212 NN denotes cell
T14618 24213-24218 NN denotes cycle
T14617 24204-24207 DT denotes the
T14616 24201-24203 IN denotes of
T14615 24192-24194 NN denotes G1
T14614 24195-24200 NN denotes stage
T14613 24188-24191 DT denotes the
T14612 24180-24187 IN denotes through
T14611 24168-24179 NN denotes progression
T14610 24163-24167 IN denotes with
T14195 22129-22131 IN denotes in
T14194 22103-22118 NN denotes preimplantation
T14193 22119-22128 NN denotes lethality
T14192 22099-22102 DT denotes the
T14191 22096-22098 IN denotes of
T14190 22166-22175 VBN denotes clarified
T14189 22079-22089 VBG denotes underlying
T14188 22090-22095 NN denotes cause
T14187 22075-22078 DT denotes The
T14186 22074-22176 sentence denotes The underlying cause of the preimplantation lethality in Capn2-/- embryos has not yet been clarified.
T14185 22073-22074 . denotes .
T14184 22065-22073 JJ denotes distinct
T14183 22052-22053 HYPH denotes -
T14182 22053-22060 NN denotes calpain
T14181 22051-22052 NN denotes m
T14180 22047-22050 CC denotes and
T14179 22038-22039 HYPH denotes -
T14178 22039-22046 NN denotes calpain
T14177 22037-22038 NN denotes μ
T14176 22034-22036 IN denotes of
T14175 22019-22023 DT denotes some
T14174 22024-22033 NNS denotes functions
T14173 22013-22018 RBS denotes least
T14172 22010-22012 RB denotes at
T14171 22061-22064 VBP denotes are
T14170 22005-22009 IN denotes that
T14169 21997-22004 VBZ denotes follows
T14168 21994-21996 PRP denotes It
T14167 21993-22074 sentence denotes It follows that at least some functions of μ-calpain and m-calpain are distinct.
T14166 21992-21993 . denotes .
T14165 21981-21982 HYPH denotes -
T14164 21982-21986 NN denotes cell
T14163 21980-21981 CD denotes 8
T14162 21987-21992 NN denotes stage
T14161 21976-21979 DT denotes the
T14040 21360-21367 NNS denotes amounts
T14039 21345-21350 PRP$ denotes their
T14038 21342-21344 IN denotes of
T14037 21332-21341 NNS denotes estimates
T14036 21323-21331 JJ denotes reliable
T14035 21311-21314 RB denotes not
T14034 21308-21310 VBP denotes do
T14033 21306-21308 , denotes ,
T14032 21285-21294 JJ denotes available
T14031 21295-21306 NN denotes methodology
T14030 21281-21284 DT denotes the
T14029 21278-21280 IN denotes in
T14028 21267-21277 NNS denotes weaknesses
T14027 21264-21266 IN denotes to
T14026 21258-21263 VBG denotes owing
T14025 21256-21258 , denotes ,
T14024 21242-21251 VBN denotes published
T14023 21252-21256 NNS denotes data
T14022 21238-21241 DT denotes the
T14021 21315-21322 VB denotes provide
T14020 21229-21237 IN denotes although
T14019 21227-21229 , denotes ,
T14018 21212-21221 JJ denotes mammalian
T14017 21222-21227 NNS denotes cells
T14016 21207-21211 JJS denotes most
T14015 21204-21206 IN denotes in
T14014 21196-21203 JJ denotes present
T14013 21192-21195 VBP denotes are
T14012 21172-21173 HYPH denotes -
T14011 21171-21172 NN denotes m
T14010 21167-21170 CC denotes and
T14009 21165-21166 HYPH denotes -
T14008 21173-21180 NN denotes calpain
T14007 21164-21165 NN denotes μ
T14006 21181-21191 NNS denotes activities
T14005 21159-21163 CC denotes Both
T14004 21158-21368 sentence denotes Both μ- and m-calpain activities are present in most mammalian cells, although the published data, owing to weaknesses in the available methodology, do not provide reliable estimates of their relative amounts.
T14003 21157-21158 . denotes .
T14002 21147-21157 NN denotes activation
T14001 21138-21146 NN denotes platelet
T14000 21135-21137 IN denotes in
T13999 21126-21134 VBN denotes involved
T13998 21117-21125 NN denotes proteins
T13997 21109-21116 JJ denotes certain
T13996 21106-21108 IN denotes of
T13995 21081-21089 NN denotes tyrosine
T13994 21090-21105 NN denotes phosphorylation
T13993 21077-21080 DT denotes the
T13992 21074-21076 IN denotes of
T13991 21068-21073 NN denotes level
T13990 21064-21067 DT denotes the
T13989 21061-21063 IN denotes at
T13988 21052-21060 RB denotes possibly
T13987 21050-21052 , denotes ,
T13986 21042-21050 NN denotes function
T13985 21033-21041 NN denotes platelet
T13984 21030-21032 IN denotes in
T13983 21018-21029 NN denotes disturbance
T13982 21016-21017 DT denotes a
T13981 20996-21006 NN denotes deficiency
T13980 20990-20995 NN denotes Capn1
T13979 20987-20989 IN denotes of
T13978 20980-20986 NN denotes result
T13977 20978-20979 DT denotes a
T13976 20975-20977 IN denotes as
T13975 20966-20974 VBN denotes observed
T13974 21007-21015 VBD denotes involved
T13973 20946-20955 JJ denotes principal
T13972 20956-20965 NN denotes phenotype
T13971 20942-20945 DT denotes The
T13970 20941-21158 sentence denotes The principal phenotype observed as a result of Capn1 deficiency involved a disturbance in platelet function, possibly at the level of the tyrosine phosphorylation of certain proteins involved in platelet activation.
T13969 20940-20941 . denotes .
T13968 20939-20940 -RRB- denotes ]
T13967 20937-20939 CD denotes 33
T13966 20936-20937 -LRB- denotes [
T13965 20931-20935 NNS denotes mice
T13964 20928-20930 IN denotes of
T13963 20918-20927 NN denotes viability
T13962 20914-20917 DT denotes the
T13961 20903-20906 RB denotes not
T13960 20899-20902 VBD denotes did
T13959 20897-20899 , denotes ,
T13958 20892-20897 NN denotes Capn1
T13957 20890-20892 , denotes ,
T13956 20878-20885 NN denotes subunit
T13955 20872-20877 JJ denotes large
T13954 20863-20864 HYPH denotes -
T13953 20864-20871 NN denotes calpain
T13952 20862-20863 NN denotes μ
T13951 20886-20890 NN denotes gene
T13950 20858-20861 DT denotes the
T13949 20855-20857 IN denotes of
T13948 20844-20854 NN denotes disruption
T13947 20833-20843 JJ denotes homozygous
T13946 20831-20833 , denotes ,
T13945 20819-20831 NN denotes implantation
T13944 20816-20818 IN denotes to
T13943 20810-20815 JJ denotes prior
T13942 20791-20797 JJ denotes murine
T13941 20798-20805 NNS denotes embryos
T13940 20786-20790 JJ denotes null
T13939 20780-20785 NN denotes Capn2
T13938 20806-20809 VBP denotes die
T13937 20772-20779 IN denotes whereas
T13936 20770-20772 , denotes ,
T13935 20907-20913 VB denotes affect
T13934 20765-20770 VBN denotes noted
T13933 20762-20764 IN denotes As
T13932 20761-20941 sentence denotes As noted, whereas Capn2 null murine embryos die prior to implantation, homozygous disruption of the μ-calpain large subunit gene, Capn1, did not affect the viability of mice [33].
T13931 20760-20761 . denotes .
T13930 20747-20760 NN denotes embryogenesis
T13929 20740-20746 IN denotes during
T13928 20734-20739 RBS denotes least
T13927 20731-20733 RB denotes at
T13926 20729-20731 , denotes ,
T13925 20710-20723 JJ denotes physiological
T13924 20701-20709 JJ denotes distinct
T13923 20724-20729 NNS denotes roles
T13922 20696-20700 DT denotes some
T13921 20682-20683 HYPH denotes -
T13920 20681-20682 NN denotes m
T13919 20677-20680 CC denotes and
T13918 20675-20676 HYPH denotes -
T13917 20683-20690 NN denotes calpain
T13916 20674-20675 NN denotes μ
T13915 20691-20695 VBP denotes have
T13914 20669-20673 IN denotes that
T13913 20667-20669 , denotes ,
T13912 20663-20667 NNS denotes mice
T13911 20660-20662 IN denotes in
T13910 20655-20659 VBN denotes done
T13909 20640-20649 NN denotes targeting
T13908 20635-20639 NN denotes gene
T13907 20650-20654 NN denotes work
T13906 20631-20634 DT denotes the
T13905 20626-20630 IN denotes from
T13904 20624-20626 , denotes ,
T13903 20619-20624 JJ denotes clear
T13902 20615-20618 RB denotes now
T13901 20612-20614 VBZ denotes is
T13900 20609-20611 PRP denotes It
T13899 20608-20761 sentence denotes It is now clear, from the gene targeting work done in mice, that μ- and m-calpain have some distinct physiological roles, at least during embryogenesis.
T13898 20607-20608 . denotes .
T13897 20602-20607 NNS denotes cells
T13896 20595-20601 IN denotes inside
T13895 20570-20584 RB denotes differentially
T13894 20566-20569 VBP denotes are
T13893 20553-20556 CD denotes two
T13892 20557-20565 NNS denotes isoforms
T13891 20549-20552 DT denotes the
T13890 20585-20594 VBN denotes regulated
T13889 20544-20548 IN denotes that
T13888 20535-20543 JJ denotes possible
T13887 20529-20531 PRP denotes it
T13886 20527-20529 , denotes ,
T13885 20517-20527 NN denotes activation
T13884 20511-20516 FW denotes vitro
T13883 20508-20510 FW denotes in
T13882 20504-20507 IN denotes for
T13881 20502-20503 SYM denotes +
T13880 20499-20502 NN denotes Ca2
T13879 20496-20498 IN denotes of
T13878 20488-20495 NNS denotes amounts
T13877 20478-20487 JJ denotes different
T13876 20465-20469 PRP denotes they
T13875 20470-20477 VBP denotes require
T13874 20459-20464 IN denotes since
T13873 20457-20459 , denotes ,
T13872 20447-20452 JJ denotes other
T13871 20453-20457 NN denotes hand
T13870 20443-20446 DT denotes the
T13869 20532-20534 VBZ denotes is
T13868 20440-20442 IN denotes On
T13867 20439-20608 sentence denotes On the other hand, since they require different amounts of Ca2+ for in vitro activation, it is possible that the two isoforms are differentially regulated inside cells.
T13866 20438-20439 . denotes .
T13865 20434-20438 FW denotes vivo
T13864 20431-20433 FW denotes in
T13863 20416-20420 JJ denotes same
T13862 20421-20430 NNS denotes functions
T13861 20412-20415 DT denotes the
T13860 20408-20411 RP denotes out
T14393 23136-23139 IN denotes for
T14392 23126-23135 JJ denotes essential
T14391 23114-23115 HYPH denotes -
T14390 23115-23122 NN denotes calpain
T14389 23113-23114 NN denotes m
T14388 23123-23125 VBZ denotes is
T14387 23108-23112 IN denotes that
T14386 23103-23107 JJ denotes true
T14385 23230-23232 VBZ denotes is
T14384 23097-23099 PRP denotes it
T14383 23100-23102 VBZ denotes is
T14382 23094-23096 IN denotes If
T14381 23093-23342 sentence denotes If it is true that m-calpain is essential for some aspect of cell viability, the survival of a few Capn2-/- embryos to the 8-cell stage is most likely due to the persistence of some maternal m-calpain mRNA and/or protein through 2–3 cell divisions.
T14380 23092-23093 . denotes .
T14379 23077-23083 JJ denotes latter
T14378 23084-23092 NN denotes category
T14377 23073-23076 DT denotes the
T13859 20402-20407 VB denotes carry
T13858 20399-20401 TO denotes to
T13857 20389-20398 NN denotes potential
T13856 20385-20388 DT denotes the
T13855 20375-20379 PRP denotes they
T13854 20380-20384 VBP denotes have
T13853 20370-20374 IN denotes that
T13852 20359-20369 VBG denotes suggesting
T13851 20357-20359 , denotes ,
T13850 20352-20357 FW denotes vitro
T13849 20349-20351 FW denotes in
T13848 20338-20348 NNS denotes substrates
T13847 20335-20337 IN denotes of
T13846 20326-20330 JJ denotes same
T13845 20331-20334 NN denotes set
T13844 20322-20325 DT denotes the
T13843 20310-20321 RB denotes essentially
T13842 20298-20302 PRP denotes they
T13841 20296-20298 , denotes ,
T13840 20303-20309 VBP denotes cleave
T13839 20289-20296 RB denotes Notably
T13838 20288-20439 sentence denotes Notably, they cleave essentially the same set of substrates in vitro, suggesting that they have the potential to carry out the same functions in vivo.
T13837 20287-20288 . denotes .
T13836 20275-20287 NN denotes biochemistry
T13835 20271-20274 CC denotes and
T13834 20261-20270 NN denotes structure
T13833 20255-20260 PRP$ denotes their
T13832 20252-20254 IN denotes in
T13831 20244-20251 JJ denotes similar
T13830 20239-20243 RB denotes very
T13829 20235-20238 VBP denotes are
T13828 20231-20234 CC denotes and
T13827 20213-20221 NN denotes sequence
T13826 20222-20230 NN denotes identity
T13825 20211-20212 NN denotes %
T13745 19803-19809 NN denotes timing
T13744 19789-19792 DT denotes The
T13743 19788-20040 sentence denotes The different timing of lethality in the Capn4 knockouts might also be a consequence of the different genetic backgrounds of the two transgenic lines, which has been observed to influence the phenotype of transgenic mice on a number of occasions [37].
T13742 19787-19788 . denotes .
T13741 19786-19787 -RRB- denotes ]
T13740 19784-19786 CD denotes 36
T13739 19783-19784 -LRB- denotes [
T13738 19777-19782 NNS denotes cells
T13737 19767-19776 JJ denotes mammalian
T13736 19764-19766 IN denotes in
T13735 19761-19763 CC denotes or
T13734 19756-19760 NNP denotes coli
T13733 19753-19755 NNP denotes E.
T13732 19750-19752 IN denotes in
T13731 19743-19749 CC denotes either
T13730 19737-19742 RB denotes alone
T13729 19722-19726 VBD denotes were
T13728 19707-19712 JJ denotes large
T13727 19713-19721 NNS denotes subunits
T13726 19699-19706 NN denotes calpain
T13725 19727-19736 VBN denotes expressed
T13724 19694-19698 WRB denotes when
T13723 19688-19693 NNS denotes hands
T13722 19684-19687 PRP$ denotes our
T13721 19681-19683 IN denotes in
T13720 19668-19671 VBD denotes was
T13719 19672-19680 VBN denotes observed
T13718 19651-19658 NN denotes calpain
T13717 19659-19667 NN denotes activity
T13716 19648-19650 DT denotes no
T13715 19644-19647 CC denotes and
T13714 19642-19644 , denotes ,
T13713 19631-19642 NN denotes possibility
T13712 19626-19630 DT denotes that
T13711 19618-19625 VB denotes exclude
T13710 19615-19617 TO denotes to
T13709 19594-19597 FW denotes al.
T13708 19591-19593 FW denotes et
T13707 19581-19590 NNP denotes Zimmerman
T13706 19598-19606 NN denotes knockout
T13705 19577-19580 DT denotes the
T13704 19607-19614 VBZ denotes appears
T13703 19568-19576 IN denotes although
T13702 19566-19568 , denotes ,
T13701 19561-19566 NNS denotes cells
T13700 19550-19560 JJ denotes eukaryotic
T13699 19547-19549 IN denotes in
T13698 19538-19546 NN denotes activity
T13697 19533-19537 DT denotes some
T13696 19519-19524 MD denotes might
T13695 19513-19518 RB denotes alone
T13694 19498-19503 JJ denotes large
T13693 19504-19512 NNS denotes subunits
T13692 19490-19497 NN denotes calpain
T13691 19525-19532 VB denotes provide
T13690 19485-19489 IN denotes that
T13689 19470-19474 VBN denotes been
T13688 19465-19469 RB denotes also
T13687 19461-19464 VBZ denotes has
T13686 19458-19460 PRP denotes It
T13685 19456-19457 -RRB- denotes )
T13684 19452-19456 NN denotes work
T13683 19440-19451 JJ denotes unpublished
T13682 19438-19440 , denotes ,
T13681 19434-19438 NNP denotes Elce
T13680 19429-19433 NNP denotes J.S.
T13679 19428-19429 -LRB- denotes (
T13678 19414-19416 VB denotes be
T13677 19417-19427 VBN denotes determined
T13676 19411-19413 TO denotes to
T13675 19407-19410 RB denotes yet
T13674 19397-19402 NNS denotes cells
T13673 19387-19396 JJ denotes mammalian
T13672 19384-19386 IN denotes in
T13671 19370-19383 NN denotes functionality
T13670 19364-19369 PRP$ denotes their
T13669 19403-19406 VBZ denotes has
T13668 19355-19363 IN denotes although
T13667 19353-19355 , denotes ,
T13666 19349-19353 NNP denotes coli
T13665 19346-19348 NNP denotes E.
T13664 19343-19345 IN denotes in
T13663 19333-19342 VBN denotes expressed
T13662 19328-19332 WRB denotes when
T13661 19311-19318 NN denotes calpain
T13660 19300-19310 JJ denotes detectable
T13659 19319-19327 NN denotes activity
T13658 19296-19299 DT denotes any
T13657 19293-19295 IN denotes to
T13656 19288-19292 NN denotes rise
T13655 19475-19484 VBN denotes suggested
T13654 19279-19282 RB denotes not
T13653 19275-19278 VBD denotes did
T13652 19260-19265 JJ denotes small
T13651 19251-19259 VBN denotes modified
T13650 19266-19274 NNS denotes subunits
T13649 19245-19250 DT denotes these
T13648 19240-19244 IN denotes with
T13647 19231-19239 NNS denotes calpains
T13646 19228-19230 IN denotes of
T13645 19217-19227 NN denotes expression
T13644 19215-19217 , denotes ,
T13643 19283-19287 VB denotes give
T13642 19208-19215 RB denotes However
T13641 19207-19788 sentence denotes However, expression of calpains with these modified small subunits did not give rise to any detectable calpain activity when expressed in E. coli, although their functionality in mammalian cells has yet to be determined (J.S. Elce, unpublished work) It has also been suggested that calpain large subunits alone might provide some activity in eukaryotic cells, although the Zimmerman et al. knockout appears to exclude that possibility, and no calpain activity was observed in our hands when calpain large subunits were expressed alone either in E. coli or in mammalian cells [36].
T13640 19206-19207 . denotes .
T13639 19198-19206 NN denotes activity
T13638 19190-19197 NN denotes calpain
T13637 19187-19189 IN denotes of
T13636 19177-19180 JJ denotes low
T13635 19181-19186 NN denotes level
T13634 19175-19176 DT denotes a
T13633 19167-19174 VB denotes support
T13632 19164-19166 TO denotes to
T13631 19153-19163 JJ denotes sufficient
T13630 19144-19149 MD denotes might
T13629 19150-19152 VB denotes be
T13628 19138-19143 WDT denotes which
T13627 19136-19138 , denotes ,
T13626 19121-19122 HYPH denotes -
T13625 19122-19130 JJ denotes terminal
T13624 19120-19121 NN denotes C
T13623 19106-19119 JJ denotes inappropriate
T13622 19131-19136 NNS denotes acids
T13621 19102-19103 SYM denotes –
T13620 19103-19105 CD denotes 30
T13619 19100-19102 CD denotes 10
T13618 19095-19099 IN denotes with
T13617 19080-19085 JJ denotes small
T13616 19072-19079 NN denotes calpain
T13615 19086-19094 NNS denotes subunits
T13614 19062-19071 JJ denotes defective
T13613 19059-19061 IN denotes to
T13612 19054-19058 NN denotes rise
T13611 19043-19048 MD denotes could
T13610 19049-19053 VB denotes give
T13609 19031-19042 NNS denotes transcripts
T13608 19025-19030 DT denotes These
T13607 19024-19207 sentence denotes These transcripts could give rise to defective calpain small subunits with 10–30 inappropriate C-terminal acids, which might be sufficient to support a low level of calpain activity.
T13606 19023-19024 . denotes .
T13605 19022-19023 -RRB- denotes ]
T13604 19020-19022 CD denotes 31
T13603 19019-19020 -LRB- denotes [
T13602 19001-19009 NN denotes promoter
T13601 18997-19000 NN denotes PGK
T13600 19010-19018 NN denotes sequence
T13599 18993-18996 DT denotes the
T13598 18990-18992 IN denotes in
T13597 18977-18983 NN denotes splice
T13596 18969-18976 JJ denotes cryptic
T13595 18959-18968 JJ denotes different
T13594 18984-18989 NNS denotes sites
T13593 18949-18954 RBS denotes least
T13592 18955-18958 CD denotes two
T13591 18946-18948 RB denotes at
T13590 18943-18945 IN denotes to
T13589 18941-18942 CD denotes 9
T13588 18936-18940 NN denotes exon
T13587 18933-18935 IN denotes of
T13586 18922-18927 JJ denotes first
T13585 18928-18932 NN denotes half
T13584 18918-18921 DT denotes the
T13583 18913-18917 IN denotes from
T13582 18905-18912 RB denotes through
T13581 18897-18904 VBG denotes reading
T13580 18895-18897 , denotes ,
T13579 18891-18892 HYPH denotes -
T13578 18892-18895 NN denotes PCR
T13577 18889-18891 NN denotes RT
T13576 18886-18888 IN denotes by
T13575 18875-18885 JJ denotes detectable
T13574 18873-18875 , denotes ,
T13573 18861-18865 NN denotes mRNA
T13572 18866-18873 NNS denotes species
T13571 18852-18860 JJ denotes multiple
T13570 18849-18851 IN denotes to
T13569 18844-18848 NN denotes rise
T13568 18839-18843 VBD denotes gave
T13567 18832-18838 NN denotes allele
T13566 18827-18831 DT denotes This
T13565 18826-19024 sentence denotes This allele gave rise to multiple mRNA species, detectable by RT-PCR, reading through from the first half of exon 9 to at least two different cryptic splice sites in the PGK promoter sequence [31].
T14160 21969-21975 IN denotes beyond
T14159 21957-21968 NN denotes development
T14158 21947-21956 JJ denotes embryonic
T14157 21943-21946 IN denotes for
T14156 21933-21942 JJ denotes essential
T14155 21921-21929 NN denotes activity
T14154 21912-21913 HYPH denotes -
T14153 21913-21920 NN denotes calpain
T14152 21911-21912 NN denotes m
T14151 21930-21932 VBZ denotes is
T14150 21906-21910 IN denotes that
T14149 21883-21884 SYM denotes -
T14148 21882-21883 HYPH denotes /
T14147 21881-21882 SYM denotes -
T14146 21885-21892 NNS denotes embryos
T14145 21876-21881 NN denotes Capn2
T14144 21873-21875 IN denotes in
T14143 21863-21872 NN denotes lethality
T14142 21861-21863 , denotes ,
T14141 21853-21861 NN denotes contrast
T14140 21893-21905 VBZ denotes demonstrates
T14139 21850-21852 IN denotes In
T14138 21849-21993 sentence denotes In contrast, lethality in Capn2-/- embryos demonstrates that m-calpain activity is essential for embryonic development beyond the 8-cell stage.
T14137 21848-21849 . denotes .
T14136 21839-21848 JJ denotes essential
T14135 21835-21838 RB denotes not
T14134 21828-21834 RB denotes simply
T14133 21815-21816 HYPH denotes -
T14132 21816-21823 NN denotes calpain
T14131 21814-21815 NN denotes μ
T14130 21811-21813 IN denotes of
T14129 21801-21810 NNS denotes functions
T14128 21797-21800 DT denotes the
T14127 21824-21827 VBP denotes are
T14126 21789-21796 IN denotes whether
T14125 21786-21788 CC denotes or
T14124 21784-21786 , denotes ,
T14123 21767-21768 HYPH denotes -
T14122 21768-21775 NN denotes calpain
T14121 21766-21767 NN denotes m
T14120 21756-21765 VBG denotes remaining
T14119 21776-21784 NN denotes activity
T14118 21752-21755 DT denotes the
T14117 21749-21751 IN denotes by
T14116 21729-21741 JJ denotes compensatory
T14115 21742-21748 NN denotes affect
T14114 21727-21728 DT denotes a
T14113 21724-21726 IN denotes to
T14112 21720-21723 IN denotes due
T14111 21707-21708 SYM denotes -
T14110 21706-21707 HYPH denotes /
T14109 21705-21706 SYM denotes -
T14108 21709-21716 NNS denotes animals
T14107 21700-21705 NN denotes Capn1
T14106 21697-21699 IN denotes in
T14105 21687-21696 NN denotes phenotype
T14104 21680-21686 JJ denotes marked
T14103 21677-21679 IN denotes of
T14102 21669-21676 NN denotes absence
T14101 21665-21668 DT denotes the
T14100 21717-21719 VBZ denotes is
T14099 21657-21664 IN denotes whether
T14098 21647-21656 VB denotes determine
T14097 21644-21646 TO denotes to
T14096 21635-21643 JJ denotes possible
T14095 21631-21634 RB denotes not
T14094 21625-21627 PRP denotes it
T14093 21623-21625 , denotes ,
T14092 21617-21623 NN denotes result
T14091 21615-21616 DT denotes a
T14090 21628-21630 VBZ denotes is
T14089 21612-21614 IN denotes As
T14088 21611-21849 sentence denotes As a result, it is not possible to determine whether the absence of marked phenotype in Capn1-/- animals is due to a compensatory affect by the remaining m-calpain activity, or whether the functions of μ-calpain are simply not essential.
T14087 21610-21611 . denotes .
T14086 21600-21604 NN denotes cell
T14085 21605-21610 NNS denotes types
T14084 21594-21599 JJ denotes other
T14083 21591-21593 IN denotes in
T14082 21586-21590 IN denotes than
T14081 21573-21585 NNS denotes erythrocytes
T14080 21569-21572 CC denotes and
T14079 21559-21568 NNS denotes platelets
T14078 21556-21558 IN denotes in
T14077 21549-21555 JJ denotes likely
T14076 21544-21548 RBR denotes less
T14075 21534-21543 RB denotes therefore
T14074 21522-21523 HYPH denotes -
T14073 21523-21530 NN denotes calpain
T14072 21521-21522 NN denotes m
T14071 21518-21520 IN denotes by
T14070 21507-21517 NN denotes deficiency
T14069 21498-21499 HYPH denotes -
T14068 21499-21506 NN denotes calpain
T14067 21497-21498 NN denotes μ
T14066 21493-21496 IN denotes for
T14065 21531-21533 VBZ denotes is
T14064 21480-21492 NN denotes Compensation
T14063 21479-21611 sentence denotes Compensation for μ-calpain deficiency by m-calpain is therefore less likely in platelets and erythrocytes than in other cell types.
T14062 21478-21479 . denotes .
T14061 21468-21478 JJ denotes detectable
T14060 21461-21467 RB denotes barely
T14059 21449-21457 NN denotes activity
T14058 21440-21441 HYPH denotes -
T14057 21441-21448 NN denotes calpain
T14056 21439-21440 NN denotes m
T14055 21458-21460 VBZ denotes is
T14054 21433-21438 IN denotes while
T14053 21431-21433 , denotes ,
T14052 21414-21415 HYPH denotes -
T14051 21415-21422 NN denotes calpain
T14050 21413-21414 NN denotes μ
T14049 21423-21431 NN denotes activity
T14048 21404-21412 JJ denotes abundant
T14047 21383-21395 NNS denotes erythrocytes
T14046 21379-21382 CC denotes and
T14045 21396-21403 VBP denotes contain
T14044 21369-21378 NNS denotes Platelets
T14043 21368-21479 sentence denotes Platelets and erythrocytes contain abundant μ-calpain activity, while m-calpain activity is barely detectable.
T14042 21367-21368 . denotes .
T14041 21351-21359 JJ denotes relative
T13564 18825-18826 . denotes .
T13563 18813-18818 NN denotes Capn4
T13562 18806-18812 NNP denotes Arthur
T13561 18819-18825 NN denotes allele
T13560 18802-18805 DT denotes the
T13559 18797-18801 IN denotes from
T13558 18789-18796 VBN denotes derived
T13557 18778-18788 NNS denotes activities
T13556 18770-18777 NN denotes calpain
T13555 18767-18769 CC denotes or
T13554 18755-18766 NNS denotes transcripts
T13553 18748-18754 VB denotes detect
T13552 18745-18747 TO denotes to
T13551 18735-18739 VBD denotes were
T13550 18740-18744 VBN denotes made
T13549 18727-18734 NNS denotes Efforts
T13548 18726-18826 sentence denotes Efforts were made to detect transcripts or calpain activities derived from the Arthur Capn4 allele.
T13547 18725-18726 . denotes .
T13546 18709-18719 JJ denotes transgenic
T13545 18703-18708 NN denotes Capn4
T13544 18699-18702 CD denotes two
T13543 18720-18725 NNS denotes lines
T13542 18695-18698 DT denotes the
T13541 18692-18694 IN denotes in
T13540 18687-18691 NN denotes work
T13539 18684-18686 IN denotes at
T13538 18675-18680 MD denotes might
T13537 18681-18683 VB denotes be
T13536 18660-18667 JJ denotes similar
T13535 18668-18674 NN denotes effect
T13534 18658-18659 DT denotes A
T13533 18657-18726 sentence denotes A similar effect might be at work in the two Capn4 transgenic lines.
T13532 18656-18657 . denotes .
T13531 18642-18648 NN denotes mutant
T13530 18649-18656 NNS denotes alleles
T13523 18598-18600 IN denotes of
T13522 18589-18597 NN denotes function
T13521 18586-18588 IN denotes of
T13520 18579-18585 NN denotes degree
T13519 18576-18578 IN denotes in
T13518 18564-18575 NNS denotes differences
T13517 18560-18563 DT denotes the
T13516 18557-18559 IN denotes to
T13515 18542-18545 VBD denotes was
T13514 18532-18541 NN denotes phenotype
T13513 18529-18531 IN denotes in
T13512 18546-18556 VBN denotes attributed
T13511 18519-18528 NN denotes variation
T13510 18515-18518 DT denotes The
T13509 18514-18657 sentence denotes The variation in phenotype was attributed to the differences in degree of function of the truncated proteins produced from the mutant alleles.
T13508 18513-18514 . denotes .
T13507 18512-18513 -RRB- denotes ]
T13506 18510-18512 CD denotes 35
T13505 18509-18510 -LRB- denotes [
T13504 18507-18508 -RRB- denotes )
T13503 18500-18502 , denotes ,
T13502 18495-18500 NN denotes E10.5
T13501 18493-18495 , denotes ,
T13500 18489-18493 NN denotes E0.5
T13499 18502-18507 NN denotes E14.5
T13498 18488-18489 -LRB- denotes (
T13497 18481-18487 NNS denotes stages
T13496 18471-18480 JJ denotes different
T13495 18468-18470 IN denotes at
T13494 18464-18467 CC denotes but
T13493 18462-18464 , denotes ,
T13492 18449-18462 NN denotes embryogenesis
T13491 18442-18448 IN denotes during
T13490 18425-18432 NNS denotes embryos
T13489 18420-18424 JJ denotes null
T13488 18409-18419 JJ denotes homozygous
T13487 18407-18409 , denotes ,
T13486 18394-18399 CD denotes three
T13485 18400-18407 NNS denotes studies
T13484 18390-18393 DT denotes all
T13483 18433-18441 VBN denotes perished
T13482 18387-18389 IN denotes In
T13481 18386-18514 sentence denotes In all three studies, homozygous null embryos perished during embryogenesis, but at different stages (E0.5, E10.5, E14.5) [35].
T13480 18385-18386 . denotes .
T13479 18379-18380 -RRB- denotes )
T13478 18376-18379 NN denotes Mll
T13477 18375-18376 -LRB- denotes (
T13476 18358-18365 NN denotes lineage
T13475 18366-18374 NN denotes leukemia
T13474 18352-18357 JJ denotes mixed
T13473 18381-18385 NN denotes gene
T13472 18348-18351 DT denotes the
T13471 18340-18347 VB denotes disrupt
T13470 18337-18339 TO denotes to
T13469 18318-18331 RB denotes independently
T13468 18313-18317 VBD denotes were
T13467 18292-18301 NN denotes targeting
T13466 18282-18291 JJ denotes different
T13465 18302-18312 NNS denotes strategies
T13464 18276-18281 CD denotes three
T13463 18274-18276 , denotes ,
T13462 18267-18274 NN denotes example
T13461 18332-18336 VBN denotes used
T13460 18263-18266 IN denotes For
T13459 18262-18386 sentence denotes For example, three different targeting strategies were independently used to disrupt the mixed lineage leukemia (Mll) gene.
T13458 18261-18262 . denotes .
T13457 18254-18261 NNS denotes studies
T13456 18243-18253 NN denotes disruption
T13455 18238-18242 NN denotes gene
T13454 18235-18237 IN denotes in
T13453 18224-18234 NNS denotes phenotypes
T13452 18214-18223 JJ denotes different
T13451 18208-18213 VB denotes yield
T13450 18205-18207 TO denotes to
T13449 18194-18198 VBN denotes been
T13448 18189-18193 VBP denotes have
T13447 18199-18204 VBN denotes shown
T13446 18168-18177 NN denotes targeting
T13445 18178-18188 NNS denotes strategies
T13444 18158-18167 JJ denotes Alternate
T13443 18157-18262 sentence denotes Alternate targeting strategies have been shown to yield different phenotypes in gene disruption studies.
T13442 18156-18157 . denotes .
T13441 18136-18147 JJ denotes hypomorphic
T13440 18148-18156 NN denotes mutation
T13439 18134-18135 DT denotes a
T13438 18127-18133 RB denotes likely
T13437 18110-18116 NNP denotes Arthur
T13436 18117-18123 NN denotes allele
T13435 18106-18109 DT denotes the
T13434 18124-18126 VBZ denotes is
T13433 18100-18105 IN denotes while
T13432 18086-18090 JJ denotes null
T13431 18081-18085 JJ denotes true
T13430 18091-18099 NN denotes genotype
T13429 18079-18080 DT denotes a
T13428 18059-18067 RB denotes probably
T13427 18068-18078 VBZ denotes represents
T13426 18042-18051 NNP denotes Zimmerman
T13425 18052-18058 NN denotes allele
T13424 18038-18041 DT denotes The
T13423 18037-18157 sentence denotes The Zimmerman allele probably represents a true null genotype while the Arthur allele is likely a hypomorphic mutation.
T13422 18036-18037 . denotes .
T13421 18026-18036 NN denotes disruption
T13420 18023-18025 IN denotes of
T13419 18015-18022 NNS denotes extents
T13418 18005-18014 JJ denotes different
T13417 18002-18004 IN denotes by
T13416 17989-17991 VB denotes be
T13415 17985-17988 MD denotes can
T13414 17973-17978 NN denotes Capn4
T13413 17964-17972 VBN denotes targeted
T13412 17960-17963 CD denotes two
T13411 17979-17984 NNS denotes lines
T13410 17954-17959 DT denotes these
T13409 17951-17953 IN denotes of
T13408 17941-17950 NN denotes lethality
T13407 17938-17940 IN denotes of
T13406 17933-17937 NN denotes time
T13405 17929-17932 DT denotes the
T13404 17926-17928 IN denotes in
T13403 17915-17925 NN denotes difference
T13402 17911-17914 DT denotes the
T13401 17992-18001 VBN denotes explained
T13400 17906-17910 IN denotes that
T13399 17897-17905 JJ denotes probable
T13398 17887-17890 RB denotes now
T13397 17891-17896 VBZ denotes seems
T13396 17884-17886 PRP denotes It
T13395 17883-18037 sentence denotes It now seems probable that the difference in the time of lethality of these two targeted Capn4 lines can be explained by different extents of disruption.
T13394 17882-17883 . denotes .
T13393 17881-17882 -RRB- denotes ]
T13392 17879-17881 CD denotes 32
T13391 17878-17879 -LRB- denotes [
T13390 17876-17877 CD denotes 8
T13389 17868-17875 IN denotes through
T13388 17866-17867 CD denotes 4
T13387 17860-17865 NNS denotes exons
T13386 17857-17859 IN denotes of
T13385 17838-17847 JJ denotes extensive
T13384 17833-17837 RBR denotes more
T13383 17828-17832 RB denotes much
T13382 17848-17856 NN denotes deletion
T13381 17826-17827 DT denotes a
T13380 17806-17816 NNS denotes colleagues
T13379 17802-17805 CC denotes and
T13378 17792-17801 NNP denotes Zimmerman
T13377 17789-17791 IN denotes by
T13376 17780-17788 VBN denotes employed
T13375 17761-17770 VBG denotes targeting
T13374 17755-17760 NN denotes Capn4
T13373 17771-17779 NN denotes strategy
T13372 17751-17754 DT denotes the
T13371 17749-17751 , denotes ,
T13370 17741-17749 NN denotes contrast
T13369 17817-17825 VBD denotes involved
T13368 17738-17740 IN denotes In
T13367 17737-17883 sentence denotes In contrast, the Capn4 targeting strategy employed by Zimmerman and colleagues involved a much more extensive deletion of exons 4 through 8 [32].
T13366 17736-17737 . denotes .
T13365 17735-17736 -RRB- denotes ]
T13364 17733-17735 CD denotes 34
T13363 17732-17733 -LRB- denotes [
T13362 17727-17731 NNP denotes coli
T13361 17724-17726 NNP denotes E.
T13360 17721-17723 IN denotes in
T13359 17707-17712 JJ denotes large
T13358 17698-17699 HYPH denotes -
T13357 17699-17706 NN denotes calpain
T13356 17697-17698 NN denotes m
T13355 17713-17720 NN denotes subunit
T13354 17693-17696 DT denotes the
T13353 17688-17692 IN denotes with
T13352 17675-17687 VBN denotes co-expressed
T13351 17670-17674 WRB denotes when
T13350 17661-17669 NN denotes activity
T13349 17653-17660 NN denotes calpain
T13348 17629-17634 JJ denotes small
T13347 17635-17642 NN denotes subunit
T13346 17625-17628 DT denotes the
T13345 17622-17624 IN denotes of
T13344 17613-17621 VBZ denotes residues
T13343 17602-17607 NN denotes amino
T13824 20209-20211 CD denotes 62
T13823 20203-20208 VBP denotes share
T13822 20191-20194 CD denotes two
T13821 20195-20202 NNS denotes enzymes
T13820 20187-20190 DT denotes The
T13819 20186-20288 sentence denotes The two enzymes share 62% sequence identity and are very similar in their structure and biochemistry.
T13818 20185-20186 . denotes .
T13817 20175-20179 FW denotes vivo
T13816 20172-20174 FW denotes in
T13815 20180-20185 NNS denotes roles
T13814 20163-20171 JJ denotes distinct
T13813 20153-20155 , denotes ,
T13812 20145-20146 HYPH denotes -
T13811 20144-20145 NN denotes m
T13810 20140-20143 CC denotes and
T13809 20138-20139 HYPH denotes -
T13808 20146-20153 NN denotes calpain
T13807 20137-20138 NN denotes μ
T13806 20135-20137 , denotes ,
T13805 20116-20126 JJ denotes ubiquitous
T13804 20112-20115 CD denotes two
T13803 20127-20135 NNS denotes isoforms
T13802 20108-20111 DT denotes the
T13801 20155-20162 VBP denotes possess
T13800 20100-20107 IN denotes whether
T13799 20091-20094 VBZ denotes has
T13798 20082-20090 NN denotes research
T13797 20074-20081 NN denotes calpain
T13796 20071-20073 IN denotes in
T13795 20052-20060 VBG denotes enduring
T13794 20061-20070 NNS denotes questions
T13793 20048-20051 DT denotes the
T13792 20045-20047 IN denotes of
T13791 20095-20099 VBN denotes been
T13790 20041-20044 CD denotes One
T13789 20040-20186 sentence denotes One of the enduring questions in calpain research has been whether the two ubiquitous isoforms, μ- and m-calpain, possess distinct in vivo roles.
T13788 20039-20040 . denotes .
T13787 20038-20039 -RRB- denotes ]
T13786 20036-20038 CD denotes 37
T13785 20035-20036 -LRB- denotes [
T13784 20025-20034 NNS denotes occasions
T13783 20022-20024 IN denotes of
T13782 20015-20021 NN denotes number
T13781 20013-20014 DT denotes a
T13780 20010-20012 IN denotes on
T13779 20005-20009 NNS denotes mice
T13778 19994-20004 JJ denotes transgenic
T13777 19991-19993 IN denotes of
T13776 19981-19990 NN denotes phenotype
T13775 19977-19980 DT denotes the
T13774 19967-19976 VB denotes influence
T13773 19964-19966 TO denotes to
T13772 19950-19954 VBN denotes been
T13771 19946-19949 VBZ denotes has
T13770 19955-19963 VBN denotes observed
T13769 19940-19945 WDT denotes which
T13768 19938-19940 , denotes ,
T13767 19922-19932 JJ denotes transgenic
T13766 19918-19921 CD denotes two
T13765 19933-19938 NNS denotes lines
T13764 19914-19917 DT denotes the
T13763 19911-19913 IN denotes of
T13762 19891-19898 JJ denotes genetic
T13761 19881-19890 JJ denotes different
T13760 19899-19910 NNS denotes backgrounds
T13759 19877-19880 DT denotes the
T13758 19874-19876 IN denotes of
T13757 19862-19873 NN denotes consequence
T13756 19860-19861 DT denotes a
T13755 19852-19856 RB denotes also
T13754 19846-19851 MD denotes might
T13753 19830-19835 NN denotes Capn4
T13752 19836-19845 NNS denotes knockouts
T13751 19826-19829 DT denotes the
T13750 19823-19825 IN denotes in
T13749 19813-19822 NN denotes lethality
T13748 19810-19812 IN denotes of
T13747 19857-19859 VB denotes be
T13746 19793-19802 JJ denotes different
T13342 17599-17601 CD denotes 25
T13341 17589-17590 HYPH denotes -
T13340 17590-17598 JJ denotes terminal
T13339 17588-17589 NN denotes C
T13338 17608-17612 NN denotes acid
T13337 17584-17587 DT denotes the
T13336 17581-17583 IN denotes of
T13335 17572-17580 NN denotes excision
T13334 17643-17652 VBD denotes abolished
T13333 17567-17571 IN denotes that
T13332 17559-17566 VBG denotes showing
T13331 17541-17542 HYPH denotes /
T13330 17542-17550 NN denotes function
T13329 17532-17541 NN denotes structure
T13328 17551-17558 NNS denotes studies
T13327 17523-17531 JJ denotes previous
T13326 17518-17522 IN denotes upon
T13325 17508-17511 VBD denotes was
T13324 17512-17517 VBN denotes based
T13323 17499-17507 NN denotes strategy
T13322 17494-17498 DT denotes This
T13321 17493-17737 sentence denotes This strategy was based upon previous structure/function studies showing that excision of the C-terminal 25 amino acid residues of the small subunit abolished calpain activity when co-expressed with the m-calpain large subunit in E. coli [34].
T13320 17492-17493 . denotes .
T13319 17491-17492 -RRB- denotes ]
T13318 17489-17491 CD denotes 31
T13317 17488-17489 -LRB- denotes [
T13316 17480-17487 NN denotes protein
T13315 17476-17479 DT denotes the
T13314 17473-17475 IN denotes of
T13313 17462-17472 NN denotes truncation
T13312 17455-17461 VBD denotes caused
T13311 17449-17454 WDT denotes which
T13310 17447-17449 , denotes ,
T13309 17446-17447 CD denotes 9
T13308 17441-17445 NN denotes exon
T13282 17296-17298 IN denotes by
T13281 17287-17295 VBN denotes employed
T13280 17321-17329 VBN denotes involved
T13279 17268-17277 NN denotes targeting
T13278 17262-17267 NN denotes Capn4
T13277 17278-17286 NN denotes strategy
T13276 17258-17261 DT denotes The
T13275 17257-17493 sentence denotes The Capn4 targeting strategy employed by Arthur and colleagues involved disrupting the C-terminus of the calpain small subunit by insertion of the PGK-Neo cassette into the middle of exon 9, which caused truncation of the protein [31].
T13274 17256-17257 . denotes .
T13273 17247-17251 JJ denotes true
T13272 17252-17256 NN denotes null
T13271 17245-17246 DT denotes a
T13270 17240-17244 IN denotes than
T13269 17233-17239 RB denotes rather
T13268 17231-17233 , denotes ,
T13267 17214-17225 JJ denotes hypomorphic
T13266 17226-17231 NN denotes state
T13265 17212-17213 DT denotes a
T13264 17189-17195 JJ denotes latter
T13263 17196-17200 NN denotes line
T13262 17185-17188 DT denotes the
T13261 17201-17211 VBZ denotes represents
T13260 17180-17184 IN denotes that
T13259 17173-17179 JJ denotes likely
T13258 17164-17166 PRP denotes it
T13257 17162-17164 , denotes ,
T13256 17152-17162 NN denotes retrospect
T13255 17167-17172 VBZ denotes seems
T13254 17149-17151 IN denotes In
T13253 17148-17257 sentence denotes In retrospect, it seems likely that the latter line represents a hypomorphic state, rather than a true null.
T13252 17147-17148 . denotes .
T13251 17146-17147 -RRB- denotes ]
T13250 17144-17146 CD denotes 31
T13249 17143-17144 -LRB- denotes [
T13248 17133-17142 VB denotes reconcile
T13247 17130-17132 TO denotes to
T13246 17120-17129 JJ denotes difficult
T13245 17115-17119 RBR denotes more
T13244 17098-17111 NN denotes mid-gestation
T13243 17095-17097 IN denotes to
T13242 17084-17094 NNS denotes colleagues
T13241 17080-17083 CC denotes and
T13240 17073-17079 NNP denotes Arthur
T13239 17070-17072 IN denotes by
T13238 17061-17069 VBN denotes reported
T13237 17047-17055 VBN denotes targeted
T13236 17038-17046 JJ denotes original
T13235 17056-17060 NN denotes line
T13234 17034-17037 DT denotes the
T13233 17029-17033 IN denotes from
T13232 17019-17020 SYM denotes -
T13231 17018-17019 HYPH denotes /
T13230 17017-17018 SYM denotes -
T13229 17021-17028 NNS denotes embryos
T13228 17012-17017 NN denotes Capn4
T13227 17009-17011 IN denotes of
T13226 17112-17114 VBZ denotes is
T13225 17000-17008 NN denotes survival
T13224 16996-16999 DT denotes The
T13223 16995-17148 sentence denotes The survival of Capn4-/- embryos from the original targeted line reported by Arthur and colleagues to mid-gestation is more difficult to reconcile [31].
T13222 16994-16995 . denotes .
T13221 16993-16994 -RRB- denotes ]
T13220 16991-16993 CD denotes 32
T13219 16990-16991 -LRB- denotes [
T13218 16976-16980 RB denotes also
T13217 16972-16975 VBD denotes was
T13216 16964-16971 NNS denotes embryos
T13215 16959-16963 JJ denotes null
T13214 16956-16958 IN denotes of
T13213 16946-16955 NN denotes lethality
T13212 16930-16945 NN denotes preimplantation
T13211 16924-16929 WDT denotes which
T13210 16981-16989 VBN denotes observed
T13209 16921-16923 IN denotes in
T13208 16906-16914 VBN denotes targeted
T13207 16900-16905 NN denotes Capn4
T13206 16896-16899 CD denotes two
T13205 16915-16920 NNS denotes lines
T13204 16892-16895 DT denotes the
T13203 16889-16891 IN denotes of
T13202 16885-16888 CD denotes one
T13201 16882-16884 IN denotes by
T13200 16872-16881 VBN denotes presented
T13199 16862-16871 NN denotes phenotype
T13198 16858-16861 DT denotes the
T13197 16853-16857 IN denotes with
T13196 16843-16852 NN denotes agreement
T13195 16840-16842 IN denotes in
T13194 16830-16836 NN denotes result
T13193 16825-16829 DT denotes this
T13192 16823-16825 , denotes ,
T13191 16815-16823 NN denotes activity
T13190 16806-16807 HYPH denotes -
T13189 16807-16814 NN denotes calpain
T13188 16805-16806 NN denotes m
T13187 16797-16804 VB denotes abolish
T13186 16794-16796 TO denotes to
T13185 16837-16839 VBZ denotes is
T13184 16780-16784 RB denotes also
T13183 16776-16779 VBD denotes was
T13182 16765-16770 NN denotes Capn4
T13181 16771-16775 NN denotes gene
T13180 16761-16764 DT denotes the
T13179 16758-16760 IN denotes of
T13178 16747-16757 NN denotes disruption
T13177 16736-16746 JJ denotes homozygous
T13176 16785-16793 VBN denotes expected
T13175 16730-16735 IN denotes Since
T13174 16729-16995 sentence denotes Since homozygous disruption of the Capn4 gene was also expected to abolish m-calpain activity, this result is in agreement with the phenotype presented by one of the two Capn4 targeted lines in which preimplantation lethality of null embryos was also observed [32].
T13173 16728-16729 . denotes .
T13172 16715-16728 NN denotes embryogenesis
T13171 16708-16714 IN denotes during
T13170 16686-16698 RB denotes specifically
T13169 16683-16685 VBZ denotes is
T13168 16674-16675 HYPH denotes -
T13167 16675-16682 NN denotes calpain
T13166 16673-16674 NN denotes m
T13165 16699-16707 VBN denotes required
T13164 16668-16672 IN denotes that
T13163 16655-16658 RB denotes now
T13162 16652-16654 TO denotes to
T13161 16659-16667 VB denotes conclude
T13160 16649-16651 PRP denotes us
T13159 16642-16648 VBZ denotes allows
T13158 16630-16641 NN denotes observation
T13157 16625-16629 DT denotes This
T13156 16624-16729 sentence denotes This observation allows us to now conclude that m-calpain is specifically required during embryogenesis.
T13155 16623-16624 . denotes .
T13154 16612-16623 NN denotes development
T13153 16609-16611 IN denotes of
T13152 16587-16602 NN denotes preimplantation
T13151 16603-16608 NN denotes stage
T13150 16583-16586 DT denotes the
T13149 16580-16582 IN denotes at
T13148 16574-16576 , denotes ,
T13147 16557-16566 JJ denotes catalytic
T13146 16548-16549 HYPH denotes -
T13145 16549-16556 NN denotes calpain
T13144 16547-16548 NN denotes m
T13143 16567-16574 NN denotes subunit
T13142 16543-16546 DT denotes the
T13141 16538-16542 VBP denotes lack
T13140 16532-16537 WDT denotes which
T13139 16530-16532 , denotes ,
T13138 16524-16525 SYM denotes -
T13137 16523-16524 HYPH denotes /
T13136 16522-16523 SYM denotes -
T13135 16526-16530 NNS denotes mice
T13134 16517-16522 NN denotes Capn2
T13133 16576-16579 VBP denotes die
T13132 16512-16516 IN denotes that
T13131 16507-16511 RB denotes here
T13130 16500-16506 VBP denotes report
T13129 16497-16499 PRP denotes We
T13128 16496-16624 sentence denotes We report here that Capn2-/- mice, which lack the m-calpain catalytic subunit, die at the preimplantation stage of development.
T13127 16495-16496 . denotes .
T13126 16486-16495 NN denotes viability
T13125 16476-16485 JJ denotes embryonic
T13124 16465-16475 VBG denotes sustaining
T13123 16461-16464 IN denotes for
T13122 16450-16460 JJ denotes sufficient
T13121 16440-16445 RB denotes alone
T13120 16432-16439 NN denotes isoform
T13119 16425-16431 DT denotes either
T13118 16446-16449 VBD denotes was
T13117 16420-16424 IN denotes that
T13116 16417-16419 CC denotes or
T13115 16415-16417 , denotes ,
T13114 16402-16415 NN denotes embryogenesis
T13113 16395-16401 IN denotes during
T13112 16373-16385 RB denotes specifically
T13111 16369-16372 VBD denotes was
T13110 16360-16361 HYPH denotes -
T13109 16359-16360 NN denotes m
T13108 16361-16368 NN denotes calpain
T13107 16352-16358 DT denotes either
T13106 16386-16394 VBN denotes required
T13105 16347-16351 IN denotes that
T13104 16329-16332 CD denotes two
T13103 16324-16328 JJ denotes last
T13102 16333-16346 NNS denotes possibilities
T13529 18638-18641 DT denotes the
T13528 18633-18637 IN denotes from
T13527 18624-18632 VBN denotes produced
T13526 18605-18614 VBN denotes truncated
T13525 18615-18623 NN denotes proteins
T13524 18601-18604 DT denotes the
T13101 16320-16323 DT denotes the
T13100 16315-16319 IN denotes with
T13099 16305-16309 VBD denotes were
T13098 16302-16304 PRP denotes we
T13097 16300-16302 , denotes ,
T13096 16295-16300 NN denotes point
T13095 16290-16294 DT denotes that
T13094 16310-16314 VBN denotes left
T13093 16287-16289 IN denotes At
T13092 16286-16496 sentence denotes At that point, we were left with the last two possibilities that either m-calpain was specifically required during embryogenesis, or that either isoform alone was sufficient for sustaining embryonic viability.
T13091 16285-16286 . denotes .
T13090 16284-16285 -RRB- denotes ]
T13089 16282-16284 CD denotes 33
T13088 16281-16282 -LRB- denotes [
T13087 16267-16271 VBD denotes were
T13086 16248-16258 NN denotes retraction
T13085 16243-16247 NN denotes clot
T13084 16239-16242 CC denotes and
T13083 16259-16266 NNS denotes defects
T13082 16227-16238 NN denotes aggregation
T13081 16218-16226 NN denotes platelet
T13080 16272-16280 VBN denotes observed
T13079 16209-16217 IN denotes although
T13078 16207-16209 , denotes ,
T13077 16200-16207 JJ denotes fertile
T13076 16196-16199 CC denotes and
T13075 16188-16195 JJ denotes healthy
T13074 16181-16183 , denotes ,
T13073 16164-16173 JJ denotes catalytic
T13072 16155-16156 HYPH denotes -
T13071 16156-16163 NN denotes calpain
T13070 16154-16155 NN denotes μ
T13069 16174-16181 NN denotes subunit
T13068 16150-16153 DT denotes the
T13067 16145-16149 VBP denotes lack
T12957 15617-15625 NN denotes knockout
T12956 15626-15630 NNS denotes mice
T12955 15611-15616 NN denotes Capn4
T12954 15608-15610 IN denotes in
T12953 15598-15607 NN denotes lethality
T12952 15588-15597 JJ denotes embryonic
T12951 15579-15587 VBD denotes observed
T12950 15554-15565 JJ denotes independent
T12949 15566-15578 NNS denotes laboratories
T12948 15550-15553 CD denotes Two
T12947 15549-15682 sentence denotes Two independent laboratories observed embryonic lethality in Capn4 knockout mice, albeit at different stages of development [31,32].
T12946 15548-15549 . denotes .
T12945 15540-15541 HYPH denotes -
T12944 15539-15540 NN denotes m
T12943 15535-15538 CC denotes and
T12942 15533-15534 HYPH denotes -
T12941 15532-15533 NN denotes μ
T12940 15541-15548 NN denotes calpain
T12939 15527-15531 CC denotes both
T12938 15524-15526 IN denotes to
T12937 15517-15523 JJ denotes common
T12936 15498-15508 JJ denotes regulatory
T12935 15492-15497 JJ denotes small
T12934 15509-15516 NN denotes subunit
T12933 15488-15491 DT denotes the
T12932 15479-15487 VBG denotes encoding
T12931 15477-15479 , denotes ,
T12930 15467-15472 NN denotes Capn4
T12929 15473-15477 NN denotes gene
T12928 15463-15466 DT denotes the
T12927 15455-15462 VB denotes disrupt
T12926 15452-15454 TO denotes to
T12925 15441-15446 RB denotes first
T12924 15437-15440 VBD denotes was
T12923 15447-15451 VBN denotes used
T12922 15432-15436 DT denotes This
T12921 15431-15549 sentence denotes This was first used to disrupt the Capn4 gene, encoding the small regulatory subunit common to both μ- and m-calpain.
T12920 15430-15431 . denotes .
T12919 15414-15421 NN denotes calpain
T12918 15422-15430 NNS denotes isoforms
T12917 15403-15413 JJ denotes individual
T12916 15400-15402 IN denotes of
T12915 15382-15393 JJ denotes physiologic
T12914 15394-15399 NNS denotes roles
T12913 15378-15381 DT denotes the
T12912 15370-15377 VB denotes examine
T12911 15367-15369 TO denotes to
T12910 15349-15357 JJ denotes powerful
T12909 15358-15366 NN denotes approach
T12908 15347-15348 DT denotes a
T12907 15334-15337 VBZ denotes has
T12906 15329-15333 NNS denotes mice
T12905 15326-15328 IN denotes in
T12904 15338-15346 VBN denotes provided
T12903 15316-15325 NN denotes targeting
T12902 15311-15315 NN denotes Gene
T12901 15310-15431 sentence denotes Gene targeting in mice has provided a powerful approach to examine the physiologic roles of individual calpain isoforms.
T12900 15309-15310 . denotes .
T12899 15288-15297 NN denotes substrate
T12898 15298-15309 NN denotes specificity
T12897 15278-15287 JJ denotes different
T12896 15273-15277 VBP denotes have
T12895 15269-15272 CC denotes and
T12894 15247-15258 RB denotes differently
T12893 15244-15246 VB denotes be
T12892 15240-15243 MD denotes may
T12891 15259-15268 VBN denotes regulated
T12890 15234-15239 WDT denotes which
T12889 15225-15233 NNS denotes isoforms
T12888 15217-15224 JJ denotes unknown
T12887 15206-15216 RB denotes previously
T12886 15203-15205 IN denotes of
T12885 15196-15202 NN denotes number
T12884 15194-15195 DT denotes a
T12883 15191-15193 IN denotes of
T12882 15181-15190 NN denotes discovery
T12881 15177-15180 DT denotes the
T12880 15174-15176 IN denotes by
T12879 15154-15161 RB denotes further
T12878 15149-15153 VBN denotes been
T12877 15145-15148 VBZ denotes has
T12876 15139-15144 NN denotes story
T12875 15135-15138 DT denotes the
T12874 15133-15135 , denotes ,
T12873 15122-15126 JJ denotes past
T12872 15127-15133 NN denotes decade
T12871 15118-15121 DT denotes the
T12870 15162-15173 VBN denotes complicated
T12869 15115-15117 IN denotes In
T12868 15114-15310 sentence denotes In the past decade, the story has been further complicated by the discovery of a number of previously unknown isoforms which may be differently regulated and have different substrate specificity.
T12867 15113-15114 . denotes .
T12866 15104-15113 NNS denotes proteases
T12865 15098-15103 JJ denotes other
T12864 15088-15097 VBG denotes affecting
T12863 15080-15087 IN denotes without
T12862 15071-15079 NNS denotes calpains
T12861 15067-15070 DT denotes the
T12860 15055-15056 HYPH denotes -
T12859 15051-15055 RB denotes down
T12858 15056-15066 VBG denotes regulating
T12857 15038-15050 RB denotes specifically
T12856 15035-15037 IN denotes of
T12855 15027-15034 JJ denotes capable
T12854 15016-15026 NNS denotes inhibitors
T12853 15013-15015 IN denotes of
T12852 15008-15012 NN denotes lack
T12851 15004-15007 DT denotes the
T12850 15001-15003 IN denotes to
T12849 14997-15000 IN denotes due
T12848 14988-14991 VBZ denotes has
T12847 14983-14987 DT denotes this
T12846 14981-14983 , denotes ,
T12845 14977-14981 NN denotes part
T12844 14971-14976 JJ denotes large
T12843 14992-14996 VBN denotes been
T12842 14968-14970 IN denotes In
T12841 14967-15114 sentence denotes In large part, this has been due to the lack of inhibitors capable of specifically down-regulating the calpains without affecting other proteases.
T12840 14966-14967 . denotes .
T12839 14959-14966 JJ denotes elusive
T12838 14946-14949 VBZ denotes has
T12837 14938-14945 NNS denotes enzymes
T12836 14934-14937 DT denotes the
T12835 14931-14933 IN denotes of
T12834 14921-14930 NNS denotes functions
T12833 14917-14920 CC denotes and
T12832 14906-14916 NNS denotes substrates
T12831 14902-14905 DT denotes the
T12830 14899-14901 IN denotes of
T12829 14879-14884 JJ denotes clear
T12828 14885-14898 NN denotes understanding
T12827 14877-14878 DT denotes a
T12826 14875-14877 , denotes ,
T12825 14872-14875 RB denotes ago
T12824 14864-14871 NNS denotes decades
T12823 14859-14863 CD denotes four
T12822 14950-14958 VBN denotes remained
T12821 14842-14847 RB denotes first
T12820 14838-14841 VBD denotes was
T12819 14829-14837 NN denotes activity
T12818 14821-14828 NN denotes calpain
T12817 14848-14858 VBN denotes identified
T12816 14812-14820 IN denotes Although
T13307 17438-17440 IN denotes of
T13306 17431-17437 NN denotes middle
T13305 17427-17430 DT denotes the
T13304 17422-17426 IN denotes into
T13303 17408-17409 HYPH denotes -
T13302 17409-17412 NN denotes Neo
T13301 17405-17408 NN denotes PGK
T13300 17413-17421 NN denotes cassette
T13299 17401-17404 DT denotes the
T13298 17398-17400 IN denotes of
T13297 17388-17397 NN denotes insertion
T13296 17385-17387 IN denotes by
T13295 17371-17376 JJ denotes small
T13294 17363-17370 NN denotes calpain
T13293 17377-17384 NN denotes subunit
T13292 17359-17362 DT denotes the
T13291 17356-17358 IN denotes of
T13290 17346-17347 HYPH denotes -
T13289 17345-17346 NN denotes C
T13288 17347-17355 NN denotes terminus
T13287 17341-17344 DT denotes the
T13286 17330-17340 VBG denotes disrupting
T13285 17310-17320 NNS denotes colleagues
T13284 17306-17309 CC denotes and
T13283 17299-17305 NNP denotes Arthur
T13066 16139-16144 WDT denotes which
T13065 16137-16139 , denotes ,
T13064 16131-16132 SYM denotes -
T13063 16130-16131 HYPH denotes /
T13062 16129-16130 SYM denotes -
T13061 16133-16137 NNS denotes mice
T13060 16124-16129 NN denotes Capn1
T13059 16183-16187 VBD denotes were
T13058 16119-16123 IN denotes that
T13057 16096-16106 JJ denotes subsequent
T13056 16107-16118 NN denotes observation
T13055 16092-16095 DT denotes the
T13054 16089-16091 IN denotes by
T13053 16084-16088 RP denotes down
T13052 16070-16074 VBD denotes were
T13051 16075-16083 VBN denotes narrowed
T13050 16062-16069 NNS denotes options
T13049 16056-16061 DT denotes These
T13048 16055-16286 sentence denotes These options were narrowed down by the subsequent observation that Capn1-/- mice, which lack the μ-calpain catalytic subunit, were healthy and fertile, although platelet aggregation and clot retraction defects were observed [33].
T13047 16054-16055 . denotes .
T13046 16042-16045 VBD denotes was
T13045 16034-16041 NN denotes isoform
T13044 16028-16033 JJ denotes other
T13043 16024-16027 DT denotes the
T13042 16021-16023 CC denotes or
T13041 16046-16054 VBN denotes required
T13040 16017-16020 CD denotes one
T13039 16013-16016 CC denotes and
T13038 16011-16013 , denotes ,
T13037 16002-16011 JJ denotes redundant
T13036 15989-15990 HYPH denotes -
T13035 15988-15989 NN denotes m
T13034 15984-15987 CC denotes and
T13033 15982-15983 HYPH denotes -
T13032 15990-15997 NN denotes calpain
T13031 15981-15982 NN denotes μ
T13030 15979-15980 -RRB- denotes )
T13029 15998-16001 VBP denotes are
T13028 15978-15979 LS denotes 4
T13027 15975-15977 CC denotes or
T13026 15973-15975 , denotes ,
T13025 15961-15964 VBD denotes was
T13024 15952-15953 HYPH denotes -
T13023 15953-15960 NN denotes calpain
T13022 15951-15952 NN denotes m
T13021 15949-15950 -RRB- denotes )
T13020 15965-15973 VBN denotes required
T13019 15948-15949 LS denotes 3
T13018 15946-15947 : denotes ;
T13017 15934-15937 VBD denotes was
T13016 15925-15926 HYPH denotes -
T13015 15926-15933 NN denotes calpain
T13014 15924-15925 NN denotes μ
T13013 15922-15923 -RRB- denotes )
T13012 15938-15946 VBN denotes required
T13011 15921-15922 LS denotes 2
T13010 15919-15920 : denotes ;
T13009 15906-15910 VBD denotes were
T13008 15897-15905 NNS denotes isoforms
T13007 15892-15896 DT denotes both
T13006 15890-15891 -RRB- denotes )
T13005 15889-15890 LS denotes 1
T13004 15887-15889 : denotes :
T13003 15876-15887 NN denotes development
T13002 15866-15875 JJ denotes embryonic
T13001 15862-15865 IN denotes for
T13000 15850-15861 NN denotes requirement
T12999 15844-15849 PRP$ denotes their
T12998 15834-15843 VBG denotes regarding
T12997 15820-15833 NNS denotes possibilities
T12996 15815-15819 CD denotes four
T12995 15805-15814 VBD denotes suggested
T12994 15793-15804 RB denotes furthermore
T12993 15789-15792 CC denotes and
T12992 15787-15789 , denotes ,
T12991 15779-15780 HYPH denotes -
T12990 15778-15779 NN denotes m
T12989 15774-15777 CC denotes and
T12988 15772-15773 HYPH denotes -
T12987 15771-15772 NN denotes μ
T12986 15780-15787 NN denotes calpain
T12985 15766-15770 CC denotes both
T12984 15762-15765 IN denotes for
T12983 15750-15752 VBZ denotes is
T12982 15736-15741 JJ denotes small
T12981 15742-15749 NN denotes subunit
T12980 15732-15735 DT denotes the
T12979 15753-15761 VBN denotes required
T12978 15727-15731 IN denotes that
T12977 15716-15726 NN denotes hypothesis
T12976 15712-15715 DT denotes the
T12975 15911-15919 VBN denotes required
T12974 15702-15711 VBD denotes supported
T12973 15689-15701 NNS denotes observations
T12972 15683-15688 DT denotes These
T12971 15682-16055 sentence denotes These observations supported the hypothesis that the small subunit is required for both μ- and m-calpain, and furthermore suggested four possibilities regarding their requirement for embryonic development: 1) both isoforms were required; 2) μ-calpain was required; 3) m-calpain was required, or 4) μ- and m-calpain are redundant, and one or the other isoform was required.
T12970 15681-15682 . denotes .
T12969 15680-15681 -RRB- denotes ]
T12968 15677-15678 , denotes ,
T12967 15675-15677 CD denotes 31
T12966 15678-15680 CD denotes 32
T12965 15674-15675 -LRB- denotes [
T12964 15662-15673 NN denotes development
T12963 15659-15661 IN denotes of
T12962 15652-15658 NNS denotes stages
T12961 15642-15651 JJ denotes different
T12960 15639-15641 IN denotes at
T12959 15632-15638 IN denotes albeit
T12958 15630-15632 , denotes ,
T7801 14568-14577 NN denotes inability
T7800 14564-14567 DT denotes The
T7799 14563-14799 sentence denotes The inability to isolate Capn2-/- ES cells, in concert with the absence of Capn2-/- embryos beyond the 8-cell stage, suggested that m-calpain activity might be essential for cell viability or the establishment of viable ES cell clones.
T7798 14562-14563 . denotes .
T7797 14545-14546 HYPH denotes -
T7796 14546-14555 JJ denotes resistant
T7795 14541-14545 NN denotes drug
T7794 14556-14562 NNS denotes clones
T7793 14537-14540 CD denotes 126
T7792 14534-14536 IN denotes of
T7791 14526-14533 NNS denotes screens
T7790 14523-14525 IN denotes in
T7789 14509-14513 VBD denotes were
T7788 14500-14502 NN denotes ES
T7787 14498-14499 SYM denotes -
T7786 14497-14498 HYPH denotes /
T7785 14496-14497 SYM denotes -
T7784 14491-14496 NN denotes Capn2
T7783 14503-14508 NNS denotes cells
T7782 14488-14490 DT denotes no
T7781 14486-14488 , denotes ,
T7780 14479-14486 RB denotes however
T7779 14477-14479 , denotes ,
T7778 14473-14477 NN denotes case
T7777 14468-14472 DT denotes this
T7776 14514-14522 VBN denotes isolated
T7775 14465-14467 IN denotes In
T7774 14464-14563 sentence denotes In this case, however, no Capn2-/- ES cells were isolated in screens of 126 drug-resistant clones.
T7773 14463-14464 . denotes .
T7772 14462-14463 -RRB- denotes ]
T7771 14460-14462 CD denotes 31
T7770 14459-14460 -LRB- denotes [
T7769 14450-14452 NN denotes ES
T7768 14448-14449 SYM denotes -
T7767 14447-14448 HYPH denotes /
T7766 14446-14447 SYM denotes +
T7765 14453-14458 NNS denotes cells
T7764 14441-14446 NN denotes Capn4
T7763 14438-14440 IN denotes of
T7762 14433-14437 NN denotes case
T7761 14429-14432 DT denotes the
T7760 14426-14428 IN denotes in
T7759 14409-14416 RB denotes readily
T7758 14417-14425 VBN denotes achieved
T7757 14405-14408 VBD denotes was
T7756 14401-14404 CC denotes and
T7755 14392-14394 NN denotes ES
T7754 14395-14400 NNS denotes cells
T7753 14383-14391 VBN denotes targeted
T7752 14380-14382 IN denotes to
T7751 14360-14371 RB denotes extensively
T7750 14355-14359 VBN denotes been
T7749 14351-14354 VBZ denotes has
T7748 14372-14379 VBN denotes applied
T7747 14341-14350 NN denotes procedure
T7746 14336-14340 DT denotes This
T7745 14335-14464 sentence denotes This procedure has been extensively applied to targeted ES cells and was readily achieved in the case of Capn4+/- ES cells [31].
T7744 14334-14335 . denotes .
T7743 14324-14334 NN denotes conversion
T7742 14319-14323 NN denotes gene
T7741 14316-14318 IN denotes by
T7740 14303-14309 NN denotes mutant
T7739 14310-14315 NNS denotes cells
T7738 14292-14302 JJ denotes homozygous
T7737 14283-14291 VB denotes generate
T7736 14280-14282 TO denotes to
T7735 14271-14279 NNS denotes attempts
T7734 14268-14270 IN denotes in
T7733 14260-14262 NN denotes mL
T7732 14259-14260 SYM denotes /
T7731 14263-14267 NN denotes G418
T7730 14257-14259 NN denotes mg
T7729 14255-14256 CD denotes 2
T7728 14252-14254 IN denotes of
T7727 14243-14251 NN denotes presence
T7726 14239-14242 DT denotes the
T7725 14236-14238 IN denotes in
T7724 14226-14235 NN denotes selection
T7723 14219-14225 JJ denotes clonal
T7722 14216-14218 IN denotes to
T7721 14201-14205 VBD denotes were
T7720 14206-14215 VBN denotes subjected
T7719 14192-14194 NN denotes ES
T7718 14190-14191 SYM denotes -
T7717 14189-14190 HYPH denotes /
T7716 14188-14189 SYM denotes +
T7715 14195-14200 NNS denotes cells
T7714 14183-14188 NN denotes Capn2
T7713 14182-14335 sentence denotes Capn2+/- ES cells were subjected to clonal selection in the presence of 2 mg/mL G418 in attempts to generate homozygous mutant cells by gene conversion.
T7712 14174-14176 NN denotes ES
T7711 14172-14173 SYM denotes -
T7710 14171-14172 HYPH denotes /
T7709 14170-14171 SYM denotes -
T7708 14177-14182 NNS denotes cells
T7707 14165-14170 NN denotes Capn2
T7706 14162-14164 IN denotes of
T7705 14151-14161 NN denotes generation
T7704 14141-14150 VBN denotes Attempted
T7358 14132-14138 NN denotes father
T7357 14128-14131 DT denotes the
T7356 14123-14127 IN denotes from
T7355 14104-14110 NN denotes mutant
T7354 14111-14117 NN denotes allele
T7353 14100-14103 DT denotes the
T7849 14798-14799 . denotes .
T7848 14787-14791 NN denotes cell
T7847 14784-14786 NN denotes ES
T7846 14792-14798 NNS denotes clones
T7845 14777-14783 JJ denotes viable
T7844 14774-14776 IN denotes of
T7843 14760-14773 NN denotes establishment
T7842 14756-14759 DT denotes the
T7841 14753-14755 CC denotes or
T7840 14743-14752 NN denotes viability
T7839 14738-14742 NN denotes cell
T7838 14734-14737 IN denotes for
T7837 14724-14733 JJ denotes essential
T7836 14715-14720 MD denotes might
T7835 14706-14714 NN denotes activity
T7834 14697-14698 HYPH denotes -
T7833 14698-14705 NN denotes calpain
T7832 14696-14697 NN denotes m
T7831 14721-14723 VB denotes be
T7830 14691-14695 IN denotes that
T7829 14679-14681 , denotes ,
T7828 14668-14669 HYPH denotes -
T7827 14669-14673 NN denotes cell
T7826 14667-14668 CD denotes 8
T7825 14674-14679 NN denotes stage
T7824 14663-14666 DT denotes the
T7823 14656-14662 IN denotes beyond
T7822 14646-14647 SYM denotes -
T7821 14645-14646 HYPH denotes /
T7820 14644-14645 SYM denotes -
T7819 14648-14655 NNS denotes embryos
T7818 14639-14644 NN denotes Capn2
T7817 14636-14638 IN denotes of
T7816 14628-14635 NN denotes absence
T7815 14624-14627 DT denotes the
T7814 14619-14623 IN denotes with
T7813 14611-14618 NN denotes concert
T7812 14608-14610 IN denotes in
T7811 14606-14608 , denotes ,
T7810 14598-14600 NN denotes ES
T7809 14596-14597 SYM denotes -
T7808 14595-14596 HYPH denotes /
T7807 14594-14595 SYM denotes -
T7806 14601-14606 NNS denotes cells
T7805 14589-14594 NN denotes Capn2
T7804 14581-14588 VB denotes isolate
T7803 14578-14580 TO denotes to
T7802 14681-14690 VBD denotes suggested
T7352 14118-14122 VBD denotes came
T7351 14095-14099 WRB denotes when
T7350 14093-14094 SYM denotes -
T7349 14092-14093 HYPH denotes /
T7348 14091-14092 SYM denotes +
T7347 14086-14091 NN denotes Capn2
T7346 14073-14080 NNS denotes females
T7345 14070-14072 IN denotes of
T7344 14068-14069 NN denotes %
T7343 14066-14068 CD denotes 55
T7342 14062-14065 CC denotes and
T7341 14056-14061 NNS denotes males
T7340 14053-14055 IN denotes of
T7339 14081-14085 VBD denotes were
T7338 14051-14052 NN denotes %
T7337 14049-14051 CD denotes 62
T7336 14045-14048 CC denotes and
T7335 14043-14045 , denotes ,
T7334 14037-14043 NN denotes mother
T7333 14033-14036 DT denotes the
T7332 14028-14032 IN denotes from
T7331 14009-14015 NN denotes mutant
T7330 14016-14022 NN denotes allele
T7329 14005-14008 DT denotes the
T7328 14023-14027 VBD denotes came
T7327 14000-14004 WRB denotes when
T7326 13998-13999 SYM denotes -
T7325 13997-13998 HYPH denotes /
T7324 13996-13997 SYM denotes +
T7323 13991-13996 NN denotes Capn2
T7322 13978-13985 NNS denotes females
T7321 13975-13977 IN denotes of
T7320 13973-13974 NN denotes %
T7319 13971-13973 CD denotes 69
T7318 13967-13970 CC denotes and
T7299 13863-13864 SYM denotes -
T7298 13862-13863 HYPH denotes /
T7297 13861-13862 SYM denotes +
T7296 13865-13872 NNS denotes animals
T7295 13856-13861 NN denotes Capn2
T7294 13853-13855 IN denotes to
T7293 13851-13852 SYM denotes +
T7292 13850-13851 HYPH denotes /
T7291 13849-13850 SYM denotes +
T7290 13844-13849 NN denotes Capn2
T7289 13841-13843 IN denotes of
T7288 13834-13840 NNS denotes ratios
T7287 13830-13833 DT denotes the
T7286 13822-13829 NNS denotes crosses
T7285 13816-13821 DT denotes these
T7284 13896-13904 VBN denotes compared
T7283 13813-13815 IN denotes In
T7282 13812-14139 sentence denotes In these crosses the ratios of Capn2+/+ to Capn2+/- animals among males or females compared well with the ratio in the combined populations; 77% of males and 69% of females were Capn2+/- when the mutant allele came from the mother, and 62% of males and 55% of females were Capn2+/- when the mutant allele came from the father.
T7281 13811-13812 . denotes .
T7280 13810-13811 -RRB- denotes )
T7279 13807-13809 CD denotes 59
T7278 13809-13810 NN denotes %
T7277 13806-13807 -LRB- denotes (
T7276 13799-13805 NN denotes father
T7275 13795-13798 DT denotes the
T7274 13790-13794 IN denotes from
T7273 13782-13784 PRP denotes it
T7272 13785-13789 VBD denotes came
T7271 13777-13781 WRB denotes when
T7270 13774-13776 IN denotes to
T7269 13765-13773 VBN denotes compared
T7268 13763-13764 -RRB- denotes )
T7267 13760-13762 CD denotes 73
T7266 13762-13763 NN denotes %
T7265 13759-13760 -LRB- denotes (
T7264 13752-13758 NN denotes mother
T7263 13748-13751 DT denotes the
T7262 13729-13735 NN denotes mutant
T7261 13736-13742 NN denotes allele
T7260 13725-13728 DT denotes the
T7259 13743-13747 VBD denotes came
T7258 13720-13724 WRB denotes when
T7257 13711-13714 VBD denotes was
T7256 13689-13690 SYM denotes -
T7255 13688-13689 HYPH denotes /
T7254 13687-13688 SYM denotes +
T7253 13691-13710 NN denotes over-representation
T7252 13682-13687 NN denotes Capn2
T7251 13679-13681 IN denotes of
T7250 13715-13719 VBN denotes seen
T7249 13665-13671 JJR denotes higher
T7248 13660-13664 RB denotes even
T7247 13672-13678 NN denotes degree
T7246 13657-13659 DT denotes An
T7245 13656-13812 sentence denotes An even higher degree of Capn2+/- over-representation was seen when the mutant allele came the mother (73%) compared to when it came from the father (59%).
T7244 13655-13656 . denotes .
T7243 13654-13655 -RRB- denotes )
T7242 13653-13654 CD denotes 2
T7241 13647-13652 NN denotes Table
T7240 13646-13647 -LRB- denotes (
T7239 13636-13637 SYM denotes -
T7238 13635-13636 HYPH denotes /
T7237 13634-13635 SYM denotes +
T7236 13638-13645 NNS denotes animals
T7235 13629-13634 NN denotes Capn2
T7234 13626-13628 IN denotes of
T7233 13606-13614 VBN denotes expected
T7232 13601-13605 IN denotes than
T7231 13593-13600 JJR denotes greater
T7230 13615-13625 NN denotes proportion
T7229 13591-13592 DT denotes a
T7228 13588-13590 IN denotes in
T7227 13574-13578 RB denotes also
T7226 13564-13565 SYM denotes -
T7225 13563-13564 HYPH denotes /
T7224 13562-13563 SYM denotes +
T7223 13557-13562 NN denotes Capn2
T7222 13553-13556 CC denotes and
T7221 13551-13552 SYM denotes +
T7220 13550-13551 HYPH denotes /
T7219 13549-13550 SYM denotes +
T7218 13566-13573 NNS denotes animals
T7217 13544-13549 NN denotes Capn2
T7216 13536-13543 IN denotes between
T7215 13579-13587 VBD denotes resulted
T7214 13528-13535 NNS denotes Crosses
T7213 13527-13656 sentence denotes Crosses between Capn2+/+ and Capn2+/- animals also resulted in a greater than expected proportion of Capn2+/- animals (Table 2).
T7212 13526-13527 . denotes .
T7211 13525-13526 -RRB- denotes ]
T7210 13523-13525 CD denotes 32
T7209 13522-13523 -LRB- denotes [
T7208 13514-13521 JJ denotes extreme
T7207 13511-13513 IN denotes as
T7206 13507-13510 RB denotes not
T7205 13486-13494 NN denotes genotype
T7204 13495-13502 NN denotes skewing
T7203 13482-13485 DT denotes the
T7202 13503-13506 VBD denotes was
T7201 13475-13481 IN denotes though
T7200 13473-13475 , denotes ,
T7199 13457-13467 JJ denotes transgenic
T7198 13451-13456 NN denotes Capn4
T7197 13468-13473 NNS denotes lines
T7196 13447-13450 DT denotes the
T7195 13444-13446 IN denotes of
T7194 13440-13443 CD denotes one
T7193 13437-13439 IN denotes in
T7192 13423-13427 RB denotes also
T7191 13419-13422 VBD denotes was
T7190 13411-13418 NNS denotes animals
T7189 13398-13410 JJ denotes heterozygous
T7188 13395-13397 IN denotes of
T7187 13367-13374 JJ denotes similar
T7186 13375-13394 NN denotes over-representation
T7185 13365-13366 DT denotes a
T7184 13363-13365 , denotes ,
T7183 13428-13436 VBN denotes reported
T7182 13350-13363 RB denotes Interestingly
T7181 13349-13527 sentence denotes Interestingly, a similar over-representation of heterozygous animals was also reported in one of the Capn4 transgenic lines, though the genotype skewing was not as extreme [32].
T7180 13348-13349 . denotes .
T7179 13347-13348 -RRB- denotes )
T7178 13346-13347 CD denotes 2
T7177 13340-13345 NN denotes Table
T7176 13339-13340 -LRB- denotes (
T7175 13314-13330 JJ denotes pre-implantation
T7174 13310-13313 CC denotes and
T7173 13292-13309 JJ denotes post-implantation
T7172 13331-13338 NNS denotes embryos
T7171 13287-13291 CC denotes both
T7170 13284-13286 IN denotes in
T7169 13276-13283 JJ denotes evident
T7168 13271-13275 RB denotes also
T7167 13257-13258 SYM denotes -
T7166 13256-13257 HYPH denotes /
T7165 13255-13256 SYM denotes +
T7164 13259-13266 NNS denotes animals
T7163 13250-13255 NN denotes Capn2
T7162 13267-13270 VBD denotes was
T7161 13230-13238 VBN denotes expected
T7160 13225-13229 IN denotes than
T7159 13218-13224 JJR denotes larger
T7158 13239-13249 NN denotes proportion
T7157 13216-13217 DT denotes A
T7156 13215-13349 sentence denotes A larger than expected proportion Capn2+/- animals was also evident in both post-implantation and pre-implantation embryos (Table 2).
T7155 13214-13215 . denotes .
T7154 13197-13207 NN denotes background
T7153 13208-13214 NN denotes strain
T7152 13192-13196 DT denotes this
T7151 13188-13191 IN denotes for
T7150 13181-13187 JJ denotes normal
T7149 13178-13180 VBZ denotes is
T7148 13172-13177 WDT denotes which
T7147 13170-13172 , denotes ,
T7146 13164-13170 NN denotes litter
T7145 13160-13163 IN denotes per
T7144 13155-13159 NNS denotes pups
T7143 13151-13154 CD denotes six
T7142 13148-13150 IN denotes of
T7141 13140-13147 NN denotes average
T7140 13137-13139 DT denotes an
T7139 13132-13136 VBD denotes were
T7138 13126-13131 EX denotes there
T7137 13122-13125 CC denotes and
T7136 13120-13122 , denotes ,
T7135 13101-13109 VBN denotes combined
T7134 13110-13120 NN denotes population
T7133 13097-13100 DT denotes the
T7132 13093-13096 IN denotes for
T7131 13086-13088 PRP denotes it
T7130 13089-13092 VBD denotes was
T7129 13083-13085 IN denotes is
T7128 13078-13082 JJ denotes same
T7127 13074-13077 DT denotes the
T7126 13062-13073 RB denotes essentially
T7125 13056-13057 -RRB- denotes )
T7124 13055-13056 NN denotes %
T7123 13053-13055 CD denotes 90
T7122 13050-13052 IN denotes to
T7121 13046-13048 CD denotes 13
T7120 13048-13049 NN denotes %
T7119 13045-13046 -LRB- denotes (
T7118 13037-13044 NNS denotes females
T7117 13034-13036 CC denotes or
T7116 13032-13033 -RRB- denotes )
T7115 13031-13032 NN denotes %
T7114 13029-13031 CD denotes 82
T7113 13026-13028 IN denotes to
T7112 13022-13024 CD denotes 14
T7111 13024-13025 NN denotes %
T7110 13021-13022 -LRB- denotes (
T7109 13015-13020 NNS denotes males
T7108 13009-13014 IN denotes among
T7107 12999-13000 SYM denotes -
T7106 12998-12999 HYPH denotes /
T7105 12997-12998 SYM denotes +
T7104 13001-13008 NNS denotes animals
T7103 12992-12997 NN denotes Capn2
T7102 12989-12991 IN denotes to
T7101 12987-12988 SYM denotes +
T7100 12986-12987 HYPH denotes /
T7099 12985-12986 SYM denotes +
T7098 12980-12985 NN denotes Capn2
T7097 12977-12979 IN denotes of
T7096 13058-13061 VBD denotes was
T7095 12971-12976 NN denotes ratio
T7094 12967-12970 DT denotes The
T7093 12966-13215 sentence denotes The ratio of Capn2+/+ to Capn2+/- animals among males (14% to 82%) or females (13% to 90%) was essentially the same is it was for the combined population, and there were an average of six pups per litter, which is normal for this background strain.
T7092 12965-12966 . denotes .
T7091 12952-12956 VBD denotes were
T7090 12942-12943 SYM denotes -
T7089 12941-12942 HYPH denotes /
T7088 12940-12941 SYM denotes +
T7087 12944-12951 NNS denotes animals
T7086 12933-12934 -RRB- denotes )
T7085 12928-12932 CD denotes 88.4
T7084 12932-12933 NN denotes %
T7317 13961-13966 NNS denotes males
T7316 13958-13960 IN denotes of
T7315 13986-13990 VBD denotes were
T7314 13956-13957 NN denotes %
T7313 13954-13956 CD denotes 77
T7312 13952-13953 : denotes ;
T7311 13932-13940 VBN denotes combined
T7310 13941-13952 NNS denotes populations
T7309 13928-13931 DT denotes the
T7308 13925-13927 IN denotes in
T7307 13919-13924 NN denotes ratio
T7306 13915-13918 DT denotes the
T7305 13910-13914 IN denotes with
T7304 13905-13909 RB denotes well
T7303 13888-13895 NNS denotes females
T7302 13885-13887 CC denotes or
T7301 13879-13884 NNS denotes males
T7300 13873-13878 IN denotes among
T7083 12927-12928 -LRB- denotes (
T7082 12935-12940 NN denotes Capn2
T7081 12923-12926 CD denotes 176
T7080 12919-12922 CC denotes and
T7079 12917-12918 -RRB- denotes )
T7078 12912-12916 CD denotes 11.6
T7077 12916-12917 NN denotes %
T7076 12911-12912 -LRB- denotes (
T7075 12909-12910 SYM denotes +
T7074 12908-12909 HYPH denotes /
T7073 12907-12908 SYM denotes +
T7072 12902-12907 NN denotes Capn2
T7071 12899-12901 CD denotes 23
T7070 12897-12899 , denotes ,
T7069 12896-12897 -RRB- denotes )
T7068 12886-12888 CD denotes 33
T7067 12889-12896 NNS denotes litters
T7066 12885-12886 -LRB- denotes (
T7065 12876-12884 NN denotes breeding
T7064 12863-12875 NN denotes heterozygote
T7063 12858-12862 IN denotes from
T7062 12850-12857 VBN denotes derived
T7061 12842-12849 NNS denotes animals
T7060 12838-12841 CD denotes 199
T7059 12835-12837 IN denotes of
T7058 12829-12834 NN denotes group
T7057 12827-12828 DT denotes a
T7056 12957-12965 VBN denotes observed
T7055 12824-12826 IN denotes In
T7054 12823-12966 sentence denotes In a group of 199 animals derived from heterozygote breeding (33 litters), 23 Capn2+/+ (11.6%) and 176 (88.4%) Capn2+/- animals were observed.
T7053 12822-12823 . denotes .
T7052 12807-12816 JJ denotes Mendelian
T7051 12805-12806 CD denotes 2
T7050 12804-12805 SYM denotes :
T7049 12803-12804 CD denotes 1
T7048 12793-12802 VBN denotes predicted
T7047 12817-12822 NN denotes ratio
T7046 12789-12792 DT denotes the
T7045 12784-12788 IN denotes than
T7044 12779-12783 JJR denotes less
T7043 12765-12778 RB denotes substantially
T7042 12735-12747 JJ denotes heterozygous
T7041 12748-12760 NNS denotes intercrosses
T7040 12728-12734 JJ denotes inbred
T7039 12723-12727 IN denotes from
T7038 12714-12715 SYM denotes -
T7037 12713-12714 HYPH denotes /
T7036 12712-12713 SYM denotes +
T7035 12715-12722 NNS denotes animals
T7034 12707-12712 NN denotes Capn2
T7033 12704-12706 IN denotes to
T7032 12702-12703 SYM denotes +
T7031 12701-12702 HYPH denotes /
T7030 12700-12701 SYM denotes +
T7029 12695-12700 NN denotes Capn2
T7028 12692-12694 IN denotes of
T7027 12686-12691 NN denotes ratio
T7026 12682-12685 DT denotes the
T7025 12680-12682 , denotes ,
T7024 12761-12764 VBD denotes was
T7023 12671-12680 RB denotes Curiously
T7022 12670-12823 sentence denotes Curiously, the ratio of Capn2+/+ to Capn2+/-animals from inbred heterozygous intercrosses was substantially less than the predicted 1:2 Mendelian ratio.
T7021 12669-12670 . denotes .
T7020 12668-12669 CD denotes 2
T7019 12662-12667 NN denotes Table
T7018 12659-12661 IN denotes in
T7017 12649-12652 VBP denotes are
T3244 11-14 DT denotes The
T3245 55-64 NNS denotes proteases
T3246 15-18 CD denotes two
T3247 19-29 JJ denotes ubiquitous
T3248 30-33 NN denotes Ca2
T3249 35-44 JJ denotes dependent
T3250 33-34 SYM denotes +
T3251 34-35 HYPH denotes -
T3252 44-46 , denotes ,
T3253 46-54 NN denotes cysteine
T3254 122-125 VBP denotes are
T3255 65-70 VBN denotes known
T3256 71-73 IN denotes as
T3257 74-75 NN denotes μ
T3258 76-83 NN denotes calpain
T3259 75-76 HYPH denotes -
T3260 84-85 -LRB- denotes (
T3261 85-92 NN denotes calpain
T3262 92-93 HYPH denotes -
T3263 93-94 CD denotes 1
T3264 94-95 -RRB- denotes )
T3265 96-99 CC denotes and
T3266 100-101 NN denotes m
T3267 102-109 NN denotes calpain
T3268 101-102 HYPH denotes -
T3269 110-111 -LRB- denotes (
T3270 111-117 NN denotes capain
T3271 117-118 HYPH denotes -
T3272 118-119 CD denotes 2
T3273 119-120 -RRB- denotes )
T3274 120-122 , denotes ,
T3275 126-129 DT denotes the
T3276 139-146 NNS denotes members
T3277 130-138 NN denotes founding
T3278 147-149 IN denotes of
T3279 150-151 DT denotes a
T3280 157-163 NN denotes family
T3281 152-156 NN denotes gene
T3282 164-174 VBG denotes comprising
T3283 175-177 CD denotes 13
T3284 178-183 NNS denotes genes
T3285 184-186 IN denotes in
T3286 187-194 NNS denotes mammals
T3287 195-196 -LRB- denotes [
T3288 196-197 CD denotes 1
T3289 197-198 SYM denotes -
T3290 198-199 CD denotes 3
T3291 199-200 -RRB- denotes ]
T3292 200-201 . denotes .
T3293 201-434 sentence denotes Both are heterodimeric enzymes consisting of distinct 80 kDa catalytic subunits, encoded by the Capn1 (μ-80 k) and Capn2 (m-80 k) genes, respectively, that associate with a common 28 kDa regulatory subunit encoded by the Capn4 gene.
T3294 202-206 DT denotes Both
T3295 207-210 VBP denotes are
T3296 211-224 JJ denotes heterodimeric
T3297 225-232 NNS denotes enzymes
T3298 233-243 VBG denotes consisting
T3299 244-246 IN denotes of
T3300 247-255 JJ denotes distinct
T3301 273-281 NNS denotes subunits
T3302 256-258 CD denotes 80
T3303 259-262 NN denotes kDa
T3304 263-272 JJ denotes catalytic
T3305 281-283 , denotes ,
T3306 283-290 VBN denotes encoded
T3307 291-293 IN denotes by
T3308 294-297 DT denotes the
T3309 332-337 NNS denotes genes
T3310 298-303 NN denotes Capn1
T3311 304-305 -LRB- denotes (
T3312 310-311 NN denotes k
T3313 305-306 NN denotes μ
T3314 306-307 HYPH denotes -
T3315 307-309 CD denotes 80
T3316 311-312 -RRB- denotes )
T3317 313-316 CC denotes and
T3318 317-322 NN denotes Capn2
T3319 323-324 -LRB- denotes (
T3320 329-330 NN denotes k
T3321 324-325 NN denotes m
T3322 325-326 HYPH denotes -
T3323 326-328 CD denotes 80
T3324 330-331 -RRB- denotes )
T3325 337-339 , denotes ,
T3326 339-351 RB denotes respectively
T3327 351-353 , denotes ,
T3328 353-357 WDT denotes that
T3329 358-367 VBP denotes associate
T3330 368-372 IN denotes with
T3331 373-374 DT denotes a
T3332 400-407 NN denotes subunit
T3333 375-381 JJ denotes common
T3334 382-384 CD denotes 28
T3335 385-388 NN denotes kDa
T3336 389-399 JJ denotes regulatory
T3337 408-415 VBN denotes encoded
T3338 416-418 IN denotes by
T3339 419-422 DT denotes the
T3340 429-433 NN denotes gene
T3341 423-428 NN denotes Capn4
T3342 433-434 . denotes .
T3343 434-604 sentence denotes The μ-80 k and m-80 k subunits share 62% amino acid sequence identity, and are very similar in terms of structure, protein chemistry, and in vitro substrate specificity.
T3344 435-438 DT denotes The
T3345 457-465 NNS denotes subunits
T3346 439-440 NN denotes μ
T3347 444-445 NN denotes k
T3348 440-441 HYPH denotes -
T3349 441-443 CD denotes 80
T3350 446-449 CC denotes and
T3351 450-451 NN denotes m
T3352 455-456 NN denotes k
T3353 451-452 HYPH denotes -
T3354 452-454 CD denotes 80
T3355 466-471 VBP denotes share
T3356 472-474 CD denotes 62
T3357 474-475 NN denotes %
T3358 496-504 NN denotes identity
T3359 476-481 NN denotes amino
T3360 482-486 NN denotes acid
T3361 487-495 NN denotes sequence
T3362 504-506 , denotes ,
T3363 506-509 CC denotes and
T3364 510-513 VBP denotes are
T3365 514-518 RB denotes very
T3366 519-526 JJ denotes similar
T3367 527-529 IN denotes in
T3368 530-535 NNS denotes terms
T3369 536-538 IN denotes of
T3370 539-548 NN denotes structure
T3371 548-550 , denotes ,
T3372 550-557 NN denotes protein
T3373 558-567 NN denotes chemistry
T3374 567-569 , denotes ,
T3375 569-572 CC denotes and
T3376 573-575 FW denotes in
T3377 576-581 FW denotes vitro
T3378 592-603 NN denotes specificity
T3379 582-591 NN denotes substrate
T3380 603-604 . denotes .
T3381 604-774 sentence denotes Despite these similarities, the differential expression patterns of μ- and m-calpain in mammalian tissues suggest they have some isoform specific and distinct functions.
T3382 605-612 IN denotes Despite
T3383 711-718 VBP denotes suggest
T3384 613-618 DT denotes these
T3385 619-631 NNS denotes similarities
T3386 631-633 , denotes ,
T3387 633-636 DT denotes the
T3388 661-669 NNS denotes patterns
T3389 637-649 JJ denotes differential
T3390 650-660 NN denotes expression
T3391 670-672 IN denotes of
T3392 673-674 NN denotes μ
T3393 682-689 NN denotes calpain
T3394 674-675 HYPH denotes -
T3395 676-679 CC denotes and
T3396 680-681 NN denotes m
T3397 681-682 HYPH denotes -
T3398 690-692 IN denotes in
T3399 693-702 JJ denotes mammalian
T3400 703-710 NNS denotes tissues
T3401 719-723 PRP denotes they
T3402 724-728 VBP denotes have
T3403 729-733 DT denotes some
T3404 764-773 NNS denotes functions
T3405 734-741 NN denotes isoform
T3406 742-750 JJ denotes specific
T3407 751-754 CC denotes and
T3408 755-763 JJ denotes distinct
T3409 773-774 . denotes .
T3410 774-936 sentence denotes The μ and m designations derive from the levels of Ca2+ required in vitro for optimal activation; 10–50 μM Ca2+ for μ-calpain and 0.3–0.35 mM Ca2+ for m-calpain.
T3411 775-778 DT denotes The
T3412 787-799 NNS denotes designations
T3413 779-780 NN denotes μ
T3414 781-784 CC denotes and
T3415 785-786 NN denotes m
T3416 800-806 VBP denotes derive
T3417 807-811 IN denotes from
T3418 812-815 DT denotes the
T3419 816-822 NNS denotes levels
T3420 823-825 IN denotes of
T3421 826-829 NN denotes Ca2
T3422 829-830 SYM denotes +
T3423 831-839 VBN denotes required
T3424 840-842 FW denotes in
T3425 843-848 FW denotes vitro
T3426 849-852 IN denotes for
T3427 853-860 JJ denotes optimal
T3428 861-871 NN denotes activation
T3429 871-872 : denotes ;
T3430 873-875 CD denotes 10
T3431 876-878 CD denotes 50
T3432 875-876 SYM denotes –
T3433 879-881 NN denotes μM
T3434 882-885 NN denotes Ca2
T3435 885-886 SYM denotes +
T3436 887-890 IN denotes for
T3437 891-892 NN denotes μ
T3438 893-900 NN denotes calpain
T3439 892-893 HYPH denotes -
T3440 901-904 CC denotes and
T3441 905-908 CD denotes 0.3
T3442 909-913 CD denotes 0.35
T3443 908-909 SYM denotes –
T3444 914-916 NN denotes mM
T3445 917-920 NN denotes Ca2
T3446 920-921 SYM denotes +
T3447 922-925 IN denotes for
T3448 926-927 NN denotes m
T3449 928-935 NN denotes calpain
T3450 927-928 HYPH denotes -
T3451 935-936 . denotes .
T3452 936-1098 sentence denotes It is generally assumed that μ- and m-calpain maintain their differential sensitivities to calcium in vivo, although this has not yet been strictly demonstrated.
T3453 937-939 PRP denotes It
T3454 953-960 VBN denotes assumed
T3455 940-942 VBZ denotes is
T3456 943-952 RB denotes generally
T3457 961-965 IN denotes that
T3458 983-991 VBP denotes maintain
T3459 966-967 NN denotes μ
T3460 975-982 NN denotes calpain
T3461 967-968 HYPH denotes -
T3462 969-972 CC denotes and
T3463 973-974 NN denotes m
T3464 974-975 HYPH denotes -
T3465 992-997 PRP$ denotes their
T3466 1011-1024 NNS denotes sensitivities
T3467 998-1010 JJ denotes differential
T3468 1025-1027 IN denotes to
T3469 1028-1035 NN denotes calcium
T3470 1036-1038 FW denotes in
T3471 1039-1043 FW denotes vivo
T3472 1043-1045 , denotes ,
T3473 1045-1053 IN denotes although
T3474 1085-1097 VBN denotes demonstrated
T3475 1054-1058 DT denotes this
T3476 1059-1062 VBZ denotes has
T3477 1063-1066 RB denotes not
T3478 1067-1070 RB denotes yet
T3479 1071-1075 VBN denotes been
T3480 1076-1084 RB denotes strictly
T3481 1097-1098 . denotes .
T3482 1098-1284 sentence denotes Furthermore, since the cytoplasmic free Ca2+ concentration is typically less than 1 μM, it is also assumed that other in vivo factors must contribute to regulation of these enzymes [3].
T3483 1099-1110 RB denotes Furthermore
T3484 1198-1205 VBN denotes assumed
T3485 1110-1112 , denotes ,
T3486 1112-1117 IN denotes since
T3487 1158-1160 VBZ denotes is
T3488 1118-1121 DT denotes the
T3489 1144-1157 NN denotes concentration
T3490 1122-1133 JJ denotes cytoplasmic
T3491 1134-1138 JJ denotes free
T3492 1139-1142 NN denotes Ca2
T3493 1142-1143 SYM denotes +
T3494 1161-1170 RB denotes typically
T3495 1171-1175 JJR denotes less
T3496 1181-1182 CD denotes 1
T3497 1176-1180 IN denotes than
T3498 1183-1185 NN denotes μM
T3499 1185-1187 , denotes ,
T3500 1187-1189 PRP denotes it
T3501 1190-1192 VBZ denotes is
T3502 1193-1197 RB denotes also
T3503 1206-1210 IN denotes that
T3504 1238-1248 VB denotes contribute
T3505 1211-1216 JJ denotes other
T3506 1225-1232 NNS denotes factors
T3507 1217-1219 FW denotes in
T3508 1220-1224 FW denotes vivo
T3509 1233-1237 MD denotes must
T3510 1249-1251 IN denotes to
T3511 1252-1262 NN denotes regulation
T3512 1263-1265 IN denotes of
T3513 1266-1271 DT denotes these
T3514 1272-1279 NNS denotes enzymes
T3515 1280-1281 -LRB- denotes [
T3516 1281-1282 CD denotes 3
T3517 1282-1283 -RRB- denotes ]
T3518 1283-1284 . denotes .
T3519 1284-1454 sentence denotes Without apriori knowledge of the factors regulating calpain activity or their relevant substrates, elucidation of biological functions for calpains presents a challenge.
T3520 1285-1292 IN denotes Without
T3521 1433-1441 VBZ denotes presents
T3522 1293-1300 FW denotes apriori
T3523 1301-1310 NN denotes knowledge
T3524 1311-1313 IN denotes of
T3525 1314-1317 DT denotes the
T3526 1318-1325 NNS denotes factors
T3527 1326-1336 VBG denotes regulating
T3528 1337-1344 NN denotes calpain
T3529 1345-1353 NN denotes activity
T3530 1354-1356 CC denotes or
T3531 1357-1362 PRP$ denotes their
T3532 1372-1382 NNS denotes substrates
T3533 1363-1371 JJ denotes relevant
T3534 1382-1384 , denotes ,
T3535 1384-1395 NN denotes elucidation
T3536 1396-1398 IN denotes of
T3537 1399-1409 JJ denotes biological
T3538 1410-1419 NNS denotes functions
T3539 1420-1423 IN denotes for
T3540 1424-1432 NNS denotes calpains
T3541 1442-1443 DT denotes a
T3542 1444-1453 NN denotes challenge
T3543 1453-1454 . denotes .
T3544 1454-1869 sentence denotes Research on calpains has linked them with a wide variety of functions including muscle growth, development, degeneration (3), neuronal growth and neurodegeneration [4], cell cycle progression [5,6], signal cascades triggered by integrins and growth factors [7], membrane protrusion [8], remodeling of the cytoskeleton and cell migration [9-15], and regulation of cell death via both necrosis and apoptosis [16-22].
T3545 1455-1463 NN denotes Research
T3546 1480-1486 VBN denotes linked
T3547 1464-1466 IN denotes on
T3548 1467-1475 NNS denotes calpains
T3549 1476-1479 VBZ denotes has
T3550 1487-1491 PRP denotes them
T3551 1492-1496 IN denotes with
T3552 1497-1498 DT denotes a
T3553 1504-1511 NN denotes variety
T3554 1499-1503 JJ denotes wide
T3555 1512-1514 IN denotes of
T3556 1515-1524 NNS denotes functions
T3557 1525-1534 VBG denotes including
T3558 1535-1541 NN denotes muscle
T3559 1542-1548 NN denotes growth
T3560 1548-1550 , denotes ,
T3561 1550-1561 NN denotes development
T3562 1561-1563 , denotes ,
T3563 1563-1575 NN denotes degeneration
T3564 1576-1577 -LRB- denotes (
T3565 1577-1578 CD denotes 3
T3566 1578-1579 -RRB- denotes )
T3567 1579-1581 , denotes ,
T3568 1581-1589 JJ denotes neuronal
T3569 1590-1596 NN denotes growth
T3570 1597-1600 CC denotes and
T3571 1601-1618 NN denotes neurodegeneration
T3572 1619-1620 -LRB- denotes [
T3573 1620-1621 CD denotes 4
T3574 1621-1622 -RRB- denotes ]
T3575 1622-1624 , denotes ,
T3576 1624-1628 NN denotes cell
T3577 1629-1634 NN denotes cycle
T3578 1635-1646 NN denotes progression
T3579 1647-1648 -LRB- denotes [
T3580 1650-1651 CD denotes 6
T3581 1648-1649 CD denotes 5
T3582 1649-1650 , denotes ,
T3583 1651-1652 -RRB- denotes ]
T3584 1652-1654 , denotes ,
T3585 1654-1660 NN denotes signal
T3586 1661-1669 NNS denotes cascades
T3587 1670-1679 VBN denotes triggered
T3588 1680-1682 IN denotes by
T3589 1683-1692 NNS denotes integrins
T3590 1693-1696 CC denotes and
T3591 1697-1703 NN denotes growth
T3592 1704-1711 NNS denotes factors
T3593 1712-1713 -LRB- denotes [
T3594 1713-1714 CD denotes 7
T3595 1714-1715 -RRB- denotes ]
T3596 1715-1717 , denotes ,
T3597 1717-1725 NN denotes membrane
T3598 1726-1736 NN denotes protrusion
T3599 1737-1738 -LRB- denotes [
T3600 1738-1739 CD denotes 8
T3601 1739-1740 -RRB- denotes ]
T3602 1740-1742 , denotes ,
T3603 1742-1752 VBG denotes remodeling
T3604 1753-1755 IN denotes of
T3605 1756-1759 DT denotes the
T3606 1760-1772 NN denotes cytoskeleton
T3607 1773-1776 CC denotes and
T3608 1777-1781 NN denotes cell
T3609 1782-1791 NN denotes migration
T3610 1792-1793 -LRB- denotes [
T3611 1793-1794 CD denotes 9
T3612 1794-1795 SYM denotes -
T3613 1795-1797 CD denotes 15
T3614 1797-1798 -RRB- denotes ]
T3615 1798-1800 , denotes ,
T3616 1800-1803 CC denotes and
T3617 1804-1814 NN denotes regulation
T3618 1815-1817 IN denotes of
T3619 1818-1822 NN denotes cell
T3620 1823-1828 NN denotes death
T3621 1829-1832 IN denotes via
T3622 1833-1837 CC denotes both
T3623 1838-1846 NN denotes necrosis
T3624 1847-1850 CC denotes and
T3625 1851-1860 NN denotes apoptosis
T3626 1861-1862 -LRB- denotes [
T3627 1862-1864 CD denotes 16
T3628 1864-1865 SYM denotes -
T3629 1865-1867 CD denotes 22
T3630 1867-1868 -RRB- denotes ]
T3631 1868-1869 . denotes .
T3632 1869-2021 sentence denotes To date, the literature suggests a complex interplay between caspases and calpains [23,24] and impact of calpain on cell death pathway components [25].
T3633 1870-1872 IN denotes To
T3634 1894-1902 VBZ denotes suggests
T3635 1873-1877 NN denotes date
T3636 1877-1879 , denotes ,
T3637 1879-1882 DT denotes the
T3638 1883-1893 NN denotes literature
T3639 1903-1904 DT denotes a
T3640 1913-1922 NN denotes interplay
T3641 1905-1912 JJ denotes complex
T3642 1923-1930 IN denotes between
T3643 1931-1939 NNS denotes caspases
T3644 1940-1943 CC denotes and
T3645 1944-1952 NNS denotes calpains
T3646 1953-1954 -LRB- denotes [
T3647 1957-1959 CD denotes 24
T3648 1954-1956 CD denotes 23
T3649 1956-1957 , denotes ,
T3650 1959-1960 -RRB- denotes ]
T3651 1961-1964 CC denotes and
T3652 1965-1971 NN denotes impact
T3653 1972-1974 IN denotes of
T3654 1975-1982 NN denotes calpain
T3655 1983-1985 IN denotes on
T3656 1986-1990 NN denotes cell
T3657 1991-1996 NN denotes death
T3658 2005-2015 NNS denotes components
T3659 1997-2004 NN denotes pathway
T3660 2016-2017 -LRB- denotes [
T3661 2017-2019 CD denotes 25
T3662 2019-2020 -RRB- denotes ]
T3663 2020-2021 . denotes .
T3664 2021-2184 sentence denotes The lack of highly specific cell-permeable inhibitors of calpains contributes to the challenge of investigating and defining calpain functions in these processes.
T3665 2022-2025 DT denotes The
T3666 2026-2030 NN denotes lack
T3667 2088-2099 VBZ denotes contributes
T3668 2031-2033 IN denotes of
T3669 2034-2040 RB denotes highly
T3670 2041-2049 JJ denotes specific
T3671 2065-2075 NNS denotes inhibitors
T3672 2050-2054 NN denotes cell
T3673 2055-2064 JJ denotes permeable
T3674 2054-2055 HYPH denotes -
T3675 2076-2078 IN denotes of
T3676 2079-2087 NNS denotes calpains
T3677 2100-2102 IN denotes to
T3678 2103-2106 DT denotes the
T3679 2107-2116 NN denotes challenge
T3680 2117-2119 IN denotes of
T3681 2120-2133 VBG denotes investigating
T3682 2134-2137 CC denotes and
T3683 2138-2146 VBG denotes defining
T3684 2147-2154 NN denotes calpain
T3685 2155-2164 NNS denotes functions
T3686 2165-2167 IN denotes in
T3687 2168-2173 DT denotes these
T3688 2174-2183 NNS denotes processes
T3689 2183-2184 . denotes .
T3690 2184-2391 sentence denotes Although over-expression of calpastatin, the endogenous protein inhibitor of μ- and m-calpain provides an important approach for these efforts, it will not distinguish isoform specific functions [24,26,27].
T3691 2185-2193 IN denotes Although
T3692 2279-2287 VBZ denotes provides
T3693 2194-2209 NN denotes over-expression
T3694 2210-2212 IN denotes of
T3695 2213-2224 NN denotes calpastatin
T3696 2224-2226 , denotes ,
T3697 2226-2229 DT denotes the
T3698 2249-2258 NN denotes inhibitor
T3699 2230-2240 JJ denotes endogenous
T3700 2241-2248 NN denotes protein
T3701 2259-2261 IN denotes of
T3702 2262-2263 NN denotes μ
T3703 2271-2278 NN denotes calpain
T3704 2263-2264 HYPH denotes -
T3705 2265-2268 CC denotes and
T3706 2269-2270 NN denotes m
T3707 2270-2271 HYPH denotes -
T3708 2341-2352 VB denotes distinguish
T3709 2288-2290 DT denotes an
T3710 2301-2309 NN denotes approach
T3711 2291-2300 JJ denotes important
T3712 2310-2313 IN denotes for
T3713 2314-2319 DT denotes these
T3714 2320-2327 NNS denotes efforts
T3715 2327-2329 , denotes ,
T3716 2329-2331 PRP denotes it
T3717 2332-2336 MD denotes will
T3718 2337-2340 RB denotes not
T3719 2353-2360 NN denotes isoform
T3720 2361-2369 JJ denotes specific
T3721 2370-2379 NNS denotes functions
T3722 2380-2381 -LRB- denotes [
T3723 2387-2389 CD denotes 27
T3724 2381-2383 CD denotes 24
T3725 2383-2384 , denotes ,
T3726 2384-2386 CD denotes 26
T3727 2386-2387 , denotes ,
T3728 2389-2390 -RRB- denotes ]
T3729 2390-2391 . denotes .
T3730 2391-2631 sentence denotes Some work has suggested isoform specific roles, such as a role for m-calpain in epidermal growth factor (EGF)-induced cell motility [28,29] and a role for μ-calpain in interferon-inducible protein 9-induced migration of keratinocytes [28].
T3731 2392-2396 DT denotes Some
T3732 2397-2401 NN denotes work
T3733 2406-2415 VBN denotes suggested
T3734 2402-2405 VBZ denotes has
T3735 2416-2423 NN denotes isoform
T3736 2424-2432 JJ denotes specific
T3737 2433-2438 NNS denotes roles
T3738 2438-2440 , denotes ,
T3739 2440-2444 JJ denotes such
T3740 2445-2447 IN denotes as
T3741 2448-2449 DT denotes a
T3742 2450-2454 NN denotes role
T3743 2455-2458 IN denotes for
T3744 2459-2460 NN denotes m
T3745 2461-2468 NN denotes calpain
T3746 2460-2461 HYPH denotes -
T3747 2469-2471 IN denotes in
T3748 2472-2481 JJ denotes epidermal
T3749 2489-2495 NN denotes factor
T3750 2482-2488 NN denotes growth
T3751 2502-2509 VBN denotes induced
T3752 2496-2497 -LRB- denotes (
T3753 2497-2500 NN denotes EGF
T3754 2500-2501 -RRB- denotes )
T3755 2501-2502 HYPH denotes -
T3756 2515-2523 NN denotes motility
T3757 2510-2514 NN denotes cell
T3758 2524-2525 -LRB- denotes [
T3759 2528-2530 CD denotes 29
T3760 2525-2527 CD denotes 28
T3761 2527-2528 , denotes ,
T3762 2530-2531 -RRB- denotes ]
T3763 2532-2535 CC denotes and
T3764 2536-2537 DT denotes a
T3765 2538-2542 NN denotes role
T3766 2543-2546 IN denotes for
T3767 2547-2548 NN denotes μ
T3768 2549-2556 NN denotes calpain
T3769 2548-2549 HYPH denotes -
T3770 2557-2559 IN denotes in
T3771 2560-2570 NN denotes interferon
T3772 2571-2580 JJ denotes inducible
T3773 2570-2571 HYPH denotes -
T3774 2599-2608 NN denotes migration
T3775 2581-2588 NN denotes protein
T3776 2591-2598 VBN denotes induced
T3777 2589-2590 CD denotes 9
T3778 2590-2591 HYPH denotes -
T3779 2609-2611 IN denotes of
T3780 2612-2625 NNS denotes keratinocytes
T3781 2626-2627 -LRB- denotes [
T3782 2627-2629 CD denotes 28
T3783 2629-2630 -RRB- denotes ]
T3784 2630-2631 . denotes .
T3785 2631-2816 sentence denotes A cell permeable calpain inhibitor (which likely inhibits other thiol-proteases as well) has been used to select cells lacking μ-calpain which display reduced proliferation rates [30].
T3786 2632-2633 DT denotes A
T3787 2657-2666 NN denotes inhibitor
T3788 2634-2638 NN denotes cell
T3789 2639-2648 JJ denotes permeable
T3790 2649-2656 NN denotes calpain
T3791 2730-2734 VBN denotes used
T3792 2667-2668 -LRB- denotes (
T3793 2668-2673 WDT denotes which
T3794 2681-2689 VBZ denotes inhibits
T3795 2674-2680 RB denotes likely
T3796 2690-2695 JJ denotes other
T3797 2702-2711 NNS denotes proteases
T3798 2696-2701 NN denotes thiol
T3799 2701-2702 HYPH denotes -
T3800 2712-2714 RB denotes as
T3801 2715-2719 RB denotes well
T3802 2719-2720 -RRB- denotes )
T3803 2721-2724 VBZ denotes has
T3804 2725-2729 VBN denotes been
T3805 2735-2737 TO denotes to
T3806 2738-2744 VB denotes select
T3807 2745-2750 NNS denotes cells
T3808 2751-2758 VBG denotes lacking
T3809 2759-2760 NN denotes μ
T3810 2761-2768 NN denotes calpain
T3811 2760-2761 HYPH denotes -
T3812 2769-2774 WDT denotes which
T3813 2775-2782 VBP denotes display
T3814 2783-2790 VBN denotes reduced
T3815 2805-2810 NNS denotes rates
T3816 2791-2804 NN denotes proliferation
T3817 2811-2812 -LRB- denotes [
T3818 2812-2814 CD denotes 30
T3819 2814-2815 -RRB- denotes ]
T3820 2815-2816 . denotes .
T3821 2816-2949 sentence denotes Interestingly, m-calpain expression persisted in these cells, suggesting a possible requirement of m-calpain for cell survival [30].
T3822 2817-2830 RB denotes Interestingly
T3823 2853-2862 VBD denotes persisted
T3824 2830-2832 , denotes ,
T3825 2832-2833 NN denotes m
T3826 2834-2841 NN denotes calpain
T3827 2833-2834 HYPH denotes -
T3828 2842-2852 NN denotes expression
T3829 2863-2865 IN denotes in
T3830 2866-2871 DT denotes these
T3831 2872-2877 NNS denotes cells
T3832 2877-2879 , denotes ,
T3833 2879-2889 VBG denotes suggesting
T3834 2890-2891 DT denotes a
T3835 2901-2912 NN denotes requirement
T3836 2892-2900 JJ denotes possible
T3837 2913-2915 IN denotes of
T3838 2916-2917 NN denotes m
T3839 2918-2925 NN denotes calpain
T3840 2917-2918 HYPH denotes -
T3841 2926-2929 IN denotes for
T3842 2930-2934 NN denotes cell
T3843 2935-2943 NN denotes survival
T3844 2944-2945 -LRB- denotes [
T3845 2945-2947 CD denotes 30
T3846 2947-2948 -RRB- denotes ]
T3847 2948-2949 . denotes .
T3848 2949-3134 sentence denotes Targeted gene deletion in mice provides a powerful approach to determining the physiological roles of μ- and m-calpain and the opportunity to approach their isoform specific functions.
T3849 2950-2958 VBN denotes Targeted
T3850 2964-2972 NN denotes deletion
T3851 2959-2963 NN denotes gene
T3852 2981-2989 VBZ denotes provides
T3853 2973-2975 IN denotes in
T3854 2976-2980 NNS denotes mice
T3855 2990-2991 DT denotes a
T3856 3001-3009 NN denotes approach
T3857 2992-3000 JJ denotes powerful
T3858 3010-3012 IN denotes to
T3859 3013-3024 VBG denotes determining
T3860 3025-3028 DT denotes the
T3861 3043-3048 NNS denotes roles
T3862 3029-3042 JJ denotes physiological
T3863 3049-3051 IN denotes of
T3864 3052-3053 NN denotes μ
T3865 3061-3068 NN denotes calpain
T3866 3053-3054 HYPH denotes -
T3867 3055-3058 CC denotes and
T3868 3059-3060 NN denotes m
T3869 3060-3061 HYPH denotes -
T3870 3069-3072 CC denotes and
T3871 3073-3076 DT denotes the
T3872 3077-3088 NN denotes opportunity
T3873 3089-3091 TO denotes to
T3874 3092-3100 VB denotes approach
T3875 3101-3106 PRP$ denotes their
T3876 3124-3133 NNS denotes functions
T3877 3107-3114 NN denotes isoform
T3878 3115-3123 JJ denotes specific
T3879 3133-3134 . denotes .
T3880 3134-3272 sentence denotes Initial studies targeted Capn4 based on the prediction that loss of this calpain subunit would abolish activity of both μ- and m-calpain.
T3881 3135-3142 JJ denotes Initial
T3882 3143-3150 NNS denotes studies
T3883 3151-3159 VBD denotes targeted
T3884 3160-3165 NN denotes Capn4
T3885 3166-3171 VBN denotes based
T3886 3172-3174 IN denotes on
T3887 3175-3178 DT denotes the
T3888 3179-3189 NN denotes prediction
T3889 3190-3194 IN denotes that
T3890 3230-3237 VB denotes abolish
T3891 3195-3199 NN denotes loss
T3892 3200-3202 IN denotes of
T3893 3203-3207 DT denotes this
T3894 3216-3223 NN denotes subunit
T3895 3208-3215 NN denotes calpain
T3896 3224-3229 MD denotes would
T3897 3238-3246 NN denotes activity
T3898 3247-3249 IN denotes of
T3899 3250-3254 CC denotes both
T3900 3255-3256 NN denotes μ
T3901 3264-3271 NN denotes calpain
T3902 3256-3257 HYPH denotes -
T3903 3258-3261 CC denotes and
T3904 3262-3263 NN denotes m
T3905 3263-3264 HYPH denotes -
T3906 3271-3272 . denotes .
T3907 3272-3425 sentence denotes Capn4-/- murine embryos died between days 10 and 11 of gestation, and there was no detectable μ- or m-calpain activity in these or younger embryos [31].
T3908 3273-3278 NN denotes Capn4
T3909 3289-3296 NNS denotes embryos
T3910 3278-3279 SYM denotes -
T3911 3279-3280 HYPH denotes /
T3912 3280-3281 SYM denotes -
T3913 3282-3288 JJ denotes murine
T3914 3297-3301 VBD denotes died
T3915 3302-3309 IN denotes between
T3916 3310-3314 NNS denotes days
T3917 3315-3317 CD denotes 10
T3918 3318-3321 CC denotes and
T3919 3322-3324 CD denotes 11
T3920 3325-3327 IN denotes of
T3921 3328-3337 NN denotes gestation
T3922 3337-3339 , denotes ,
T3923 3339-3342 CC denotes and
T3924 3343-3348 EX denotes there
T3925 3349-3352 VBD denotes was
T3926 3353-3355 DT denotes no
T3927 3383-3391 NN denotes activity
T3928 3356-3366 JJ denotes detectable
T3929 3367-3368 NN denotes μ
T3930 3375-3382 NN denotes calpain
T3931 3368-3369 HYPH denotes -
T3932 3370-3372 CC denotes or
T3933 3373-3374 NN denotes m
T3934 3374-3375 HYPH denotes -
T3935 3392-3394 IN denotes in
T3936 3395-3400 DT denotes these
T3937 3401-3403 CC denotes or
T3938 3404-3411 JJR denotes younger
T3939 3412-3419 NNS denotes embryos
T3940 3420-3421 -LRB- denotes [
T3941 3421-3423 CD denotes 31
T3942 3423-3424 -RRB- denotes ]
T3943 3424-3425 . denotes .
T3944 3425-3758 sentence denotes Capn4-/- murine embryonic fibroblasts (MEFs) could be cultured from these embryos, although they also lacked calpain activity as assessed by casein zymography or by the formation of characteristic spectrin breakdown products, and they displayed migration defects consistent with a role for calpain in release of focal adhesions [9].
T3945 3426-3431 NN denotes Capn4
T3946 3480-3488 VBN denotes cultured
T3947 3431-3432 SYM denotes -
T3948 3432-3433 HYPH denotes /
T3949 3433-3434 SYM denotes -
T3950 3435-3441 JJ denotes murine
T3951 3452-3463 NNS denotes fibroblasts
T3952 3442-3451 JJ denotes embryonic
T3953 3464-3465 -LRB- denotes (
T3954 3465-3469 NNS denotes MEFs
T3955 3469-3470 -RRB- denotes )
T3956 3471-3476 MD denotes could
T3957 3477-3479 VB denotes be
T3958 3489-3493 IN denotes from
T3959 3494-3499 DT denotes these
T3960 3500-3507 NNS denotes embryos
T3961 3507-3509 , denotes ,
T3962 3509-3517 IN denotes although
T3963 3528-3534 VBD denotes lacked
T3964 3518-3522 PRP denotes they
T3965 3523-3527 RB denotes also
T3966 3535-3542 NN denotes calpain
T3967 3543-3551 NN denotes activity
T3968 3552-3554 IN denotes as
T3969 3555-3563 VBN denotes assessed
T3970 3564-3566 IN denotes by
T3971 3567-3573 NN denotes casein
T3972 3574-3584 NN denotes zymography
T3973 3585-3587 CC denotes or
T3974 3588-3590 IN denotes by
T3975 3591-3594 DT denotes the
T3976 3595-3604 NN denotes formation
T3977 3605-3607 IN denotes of
T3978 3608-3622 JJ denotes characteristic
T3979 3642-3650 NNS denotes products
T3980 3623-3631 NN denotes spectrin
T3981 3632-3641 NN denotes breakdown
T3982 3650-3652 , denotes ,
T3983 3652-3655 CC denotes and
T3984 3656-3660 PRP denotes they
T3985 3661-3670 VBD denotes displayed
T3986 3671-3680 NN denotes migration
T3987 3681-3688 NNS denotes defects
T3988 3689-3699 JJ denotes consistent
T3989 3700-3704 IN denotes with
T3990 3705-3706 DT denotes a
T3991 3707-3711 NN denotes role
T3992 3712-3715 IN denotes for
T3993 3716-3723 NN denotes calpain
T3994 3724-3726 IN denotes in
T3995 3727-3734 NN denotes release
T3996 3735-3737 IN denotes of
T3997 3738-3743 JJ denotes focal
T3998 3744-3753 NNS denotes adhesions
T3999 3754-3755 -LRB- denotes [
T4000 3755-3756 CD denotes 9
T4001 3756-3757 -RRB- denotes ]
T4002 3757-3758 . denotes .
T4003 3758-3937 sentence denotes An independently derived Capn4 knockout, involving a more extensive deletion of the gene, resulted in an earlier embryonic lethality, apparently at a pre-implantation stage [32].
T4004 3759-3761 DT denotes An
T4005 3790-3798 NN denotes knockout
T4006 3762-3775 RB denotes independently
T4007 3776-3783 VBN denotes derived
T4008 3784-3789 NN denotes Capn4
T4009 3849-3857 VBD denotes resulted
T4010 3798-3800 , denotes ,
T4011 3800-3809 VBG denotes involving
T4012 3810-3811 DT denotes a
T4013 3827-3835 NN denotes deletion
T4014 3812-3816 RBR denotes more
T4015 3817-3826 JJ denotes extensive
T4016 3836-3838 IN denotes of
T4017 3839-3842 DT denotes the
T4018 3843-3847 NN denotes gene
T4019 3847-3849 , denotes ,
T4020 3858-3860 IN denotes in
T4021 3861-3863 DT denotes an
T4022 3882-3891 NN denotes lethality
T4023 3864-3871 JJR denotes earlier
T4024 3872-3881 JJ denotes embryonic
T4025 3891-3893 , denotes ,
T4026 3893-3903 RB denotes apparently
T4027 3904-3906 IN denotes at
T4028 3907-3908 DT denotes a
T4029 3926-3931 NN denotes stage
T4030 3909-3925 JJ denotes pre-implantation
T4031 3932-3933 -LRB- denotes [
T4032 3933-3935 CD denotes 32
T4033 3935-3936 -RRB- denotes ]
T4034 3936-3937 . denotes .
T4035 3937-4254 sentence denotes The different times of embryonic lethality suggested that the first reported Capn4-/- mice [31] were targeted with a hypomorphic mutation, which retained some small level of calpain activity, allowing for their survival to mid-gestation, while the second reported Capn4-/- mice [32] represented a true null mutation.
T4036 3938-3941 DT denotes The
T4037 3952-3957 NNS denotes times
T4038 3942-3951 JJ denotes different
T4039 3981-3990 VBD denotes suggested
T4040 3958-3960 IN denotes of
T4041 3961-3970 JJ denotes embryonic
T4042 3971-3980 NN denotes lethality
T4043 3991-3995 IN denotes that
T4044 4039-4047 VBN denotes targeted
T4045 3996-3999 DT denotes the
T4046 4024-4028 NNS denotes mice
T4047 4000-4005 JJ denotes first
T4048 4006-4014 VBN denotes reported
T4049 4015-4020 NN denotes Capn4
T4050 4020-4021 SYM denotes -
T4051 4021-4022 HYPH denotes /
T4052 4022-4023 SYM denotes -
T4053 4029-4030 -LRB- denotes [
T4054 4030-4032 CD denotes 31
T4055 4032-4033 -RRB- denotes ]
T4056 4034-4038 VBD denotes were
T4057 4048-4052 IN denotes with
T4058 4053-4054 DT denotes a
T4059 4067-4075 NN denotes mutation
T4060 4055-4066 JJ denotes hypomorphic
T4061 4075-4077 , denotes ,
T4062 4077-4082 WDT denotes which
T4063 4083-4091 VBD denotes retained
T4064 4092-4096 DT denotes some
T4065 4103-4108 NN denotes level
T4066 4097-4102 JJ denotes small
T4067 4109-4111 IN denotes of
T4068 4112-4119 NN denotes calpain
T4069 4120-4128 NN denotes activity
T4070 4128-4130 , denotes ,
T4071 4130-4138 VBG denotes allowing
T4072 4139-4142 IN denotes for
T4073 4143-4148 PRP$ denotes their
T4074 4149-4157 NN denotes survival
T4075 4158-4160 IN denotes to
T4076 4161-4174 NN denotes mid-gestation
T4077 4174-4176 , denotes ,
T4078 4176-4181 IN denotes while
T4079 4221-4232 VBD denotes represented
T4080 4182-4185 DT denotes the
T4081 4211-4215 NNS denotes mice
T4082 4186-4192 JJ denotes second
T4083 4193-4201 VBN denotes reported
T4084 4202-4207 NN denotes Capn4
T4085 4207-4208 SYM denotes -
T4086 4208-4209 HYPH denotes /
T4087 4209-4210 SYM denotes -
T4088 4216-4217 -LRB- denotes [
T4089 4217-4219 CD denotes 32
T4090 4219-4220 -RRB- denotes ]
T4091 4233-4234 DT denotes a
T4092 4245-4253 NN denotes mutation
T4093 4235-4239 JJ denotes true
T4094 4240-4244 JJ denotes null
T4095 4253-4254 . denotes .
T4096 4254-4488 sentence denotes Disruption of Capn1, encoding the μ-calpain catalytic subunit, was subsequently reported to result in fertile, viable mice with some mild defects in the μ-calpain rich platelets relating to their aggregation and clot retraction [33].
T4097 4255-4265 NN denotes Disruption
T4098 4335-4343 VBN denotes reported
T4099 4266-4268 IN denotes of
T4100 4269-4274 NN denotes Capn1
T4101 4274-4276 , denotes ,
T4102 4276-4284 VBG denotes encoding
T4103 4285-4288 DT denotes the
T4104 4309-4316 NN denotes subunit
T4105 4289-4290 NN denotes μ
T4106 4291-4298 NN denotes calpain
T4107 4290-4291 HYPH denotes -
T4108 4299-4308 JJ denotes catalytic
T4109 4316-4318 , denotes ,
T4110 4318-4321 VBD denotes was
T4111 4322-4334 RB denotes subsequently
T4112 4344-4346 TO denotes to
T4113 4347-4353 VB denotes result
T4114 4354-4356 IN denotes in
T4115 4357-4364 JJ denotes fertile
T4116 4373-4377 NNS denotes mice
T4117 4364-4366 , denotes ,
T4118 4366-4372 JJ denotes viable
T4119 4378-4382 IN denotes with
T4120 4383-4387 DT denotes some
T4121 4393-4400 NNS denotes defects
T4122 4388-4392 JJ denotes mild
T4123 4401-4403 IN denotes in
T4124 4404-4407 DT denotes the
T4125 4423-4432 NNS denotes platelets
T4126 4408-4409 NN denotes μ
T4127 4410-4417 NN denotes calpain
T4128 4409-4410 HYPH denotes -
T4129 4418-4422 JJ denotes rich
T4130 4433-4441 VBG denotes relating
T4131 4442-4444 IN denotes to
T4132 4445-4450 PRP$ denotes their
T4133 4451-4462 NN denotes aggregation
T4134 4463-4466 CC denotes and
T4135 4467-4471 NN denotes clot
T4136 4472-4482 NN denotes retraction
T4137 4483-4484 -LRB- denotes [
T4138 4484-4486 CD denotes 33
T4139 4486-4487 -RRB- denotes ]
T4140 4487-4488 . denotes .
T4141 4488-4636 sentence denotes The fact that Capn4 null mice die during embryogenesis indicates that at least one of the ubiquitous calpains is essential for development to term.
T4142 4489-4492 DT denotes The
T4143 4493-4497 NN denotes fact
T4144 4544-4553 VBZ denotes indicates
T4145 4498-4502 IN denotes that
T4146 4519-4522 VBP denotes die
T4147 4503-4508 NN denotes Capn4
T4148 4509-4513 JJ denotes null
T4149 4514-4518 NNS denotes mice
T4150 4523-4529 IN denotes during
T4151 4530-4543 NN denotes embryogenesis
T4152 4554-4558 IN denotes that
T4153 4599-4601 VBZ denotes is
T4154 4559-4561 RB denotes at
T4155 4568-4571 CD denotes one
T4156 4562-4567 RBS denotes least
T4157 4572-4574 IN denotes of
T4158 4575-4578 DT denotes the
T4159 4590-4598 NNS denotes calpains
T4160 4579-4589 JJ denotes ubiquitous
T4161 4602-4611 JJ denotes essential
T4162 4612-4615 IN denotes for
T4163 4616-4627 NN denotes development
T4164 4628-4630 IN denotes to
T4165 4631-4635 NN denotes term
T4166 4635-4636 . denotes .
T4167 4636-4899 sentence denotes The viability of Capn1-deficient mice does not however distinguish between two possibilities: either that m-calpain is specifically required during embryogenesis, or that either form of calpain alone is sufficient and can compensate for the absence of the other.
T4168 4637-4640 DT denotes The
T4169 4641-4650 NN denotes viability
T4170 4692-4703 VB denotes distinguish
T4171 4651-4653 IN denotes of
T4172 4654-4659 NN denotes Capn1
T4173 4660-4669 JJ denotes deficient
T4174 4659-4660 HYPH denotes -
T4175 4670-4674 NNS denotes mice
T4176 4675-4679 VBZ denotes does
T4177 4680-4683 RB denotes not
T4178 4684-4691 RB denotes however
T4179 4704-4711 IN denotes between
T4180 4712-4715 CD denotes two
T4181 4716-4729 NNS denotes possibilities
T4182 4729-4731 : denotes :
T4183 4731-4737 CC denotes either
T4184 4769-4777 VBN denotes required
T4185 4738-4742 IN denotes that
T4186 4743-4744 NN denotes m
T4187 4745-4752 NN denotes calpain
T4188 4744-4745 HYPH denotes -
T4189 4753-4755 VBZ denotes is
T4190 4756-4768 RB denotes specifically
T4191 4778-4784 IN denotes during
T4192 4785-4798 NN denotes embryogenesis
T4193 4798-4800 , denotes ,
T4194 4800-4802 CC denotes or
T4195 4803-4807 IN denotes that
T4196 4837-4839 VBZ denotes is
T4197 4808-4814 CC denotes either
T4198 4815-4819 NN denotes form
T4199 4820-4822 IN denotes of
T4200 4823-4830 NN denotes calpain
T4201 4831-4836 RB denotes alone
T4202 4840-4850 JJ denotes sufficient
T4203 4851-4854 CC denotes and
T4204 4855-4858 MD denotes can
T4205 4859-4869 VB denotes compensate
T4206 4870-4873 IN denotes for
T4207 4874-4877 DT denotes the
T4208 4878-4885 NN denotes absence
T4209 4886-4888 IN denotes of
T4210 4889-4892 DT denotes the
T4211 4893-4898 JJ denotes other
T4212 4898-4899 . denotes .
T4213 4899-5001 sentence denotes To resolve this question, we have now knocked out the Capn2 gene encoding the m-80 k subunit in mice.
T4214 4900-4902 TO denotes To
T4215 4903-4910 VB denotes resolve
T4216 4938-4945 VBN denotes knocked
T4217 4911-4915 DT denotes this
T4218 4916-4924 NN denotes question
T4219 4924-4926 , denotes ,
T4220 4926-4928 PRP denotes we
T4221 4929-4933 VBP denotes have
T4222 4934-4937 RB denotes now
T4223 4946-4949 RP denotes out
T4224 4950-4953 DT denotes the
T4225 4960-4964 NN denotes gene
T4226 4954-4959 NN denotes Capn2
T4227 4965-4973 VBG denotes encoding
T4228 4974-4977 DT denotes the
T4229 4985-4992 NN denotes subunit
T4230 4978-4979 NN denotes m
T4231 4983-4984 NN denotes k
T4232 4979-4980 HYPH denotes -
T4233 4980-4982 CD denotes 80
T4234 4993-4995 IN denotes in
T4235 4996-5000 NNS denotes mice
T4236 5000-5001 . denotes .
T4237 5001-5146 sentence denotes We report here that Capn2 null embryos died prior to the implantation stage, indicating that m-calpain is indispensable for early embryogenesis.
T4238 5002-5004 PRP denotes We
T4239 5005-5011 VBP denotes report
T4240 5012-5016 RB denotes here
T4241 5017-5021 IN denotes that
T4242 5041-5045 VBD denotes died
T4243 5022-5027 NN denotes Capn2
T4244 5028-5032 JJ denotes null
T4245 5033-5040 NNS denotes embryos
T4246 5046-5051 JJ denotes prior
T4247 5052-5054 IN denotes to
T4248 5055-5058 DT denotes the
T4249 5072-5077 NN denotes stage
T4250 5059-5071 NN denotes implantation
T4251 5077-5079 , denotes ,
T4252 5079-5089 VBG denotes indicating
T4253 5090-5094 IN denotes that
T4254 5105-5107 VBZ denotes is
T4255 5095-5096 NN denotes m
T4256 5097-5104 NN denotes calpain
T4257 5096-5097 HYPH denotes -
T4258 5108-5121 JJ denotes indispensable
T4259 5122-5125 IN denotes for
T4260 5126-5131 JJ denotes early
T4261 5132-5145 NN denotes embryogenesis
T4262 5145-5146 . denotes .
T4263 5146-5300 sentence denotes This role cannot be fulfilled by μ-calpain, which is expressed in embryonic stem (ES) cells [31] and is assumed to be present at this stage of gestation.
T4264 5147-5151 DT denotes This
T4265 5152-5156 NN denotes role
T4266 5167-5176 VBN denotes fulfilled
T4267 5157-5160 MD denotes can
T4268 5160-5163 RB denotes not
T4269 5164-5166 VB denotes be
T4270 5177-5179 IN denotes by
T4271 5180-5181 NN denotes μ
T4272 5182-5189 NN denotes calpain
T4273 5181-5182 HYPH denotes -
T4274 5189-5191 , denotes ,
T4275 5191-5196 WDT denotes which
T4276 5200-5209 VBN denotes expressed
T4277 5197-5199 VBZ denotes is
T4278 5210-5212 IN denotes in
T4279 5213-5222 JJ denotes embryonic
T4280 5223-5227 NN denotes stem
T4281 5233-5238 NNS denotes cells
T4282 5228-5229 -LRB- denotes (
T4283 5229-5231 NN denotes ES
T4284 5231-5232 -RRB- denotes )
T4285 5239-5240 -LRB- denotes [
T4286 5240-5242 CD denotes 31
T4287 5242-5243 -RRB- denotes ]
T4288 5244-5247 CC denotes and
T4289 5248-5250 VBZ denotes is
T4290 5251-5258 VBN denotes assumed
T4291 5259-5261 TO denotes to
T4292 5262-5264 VB denotes be
T4293 5265-5272 JJ denotes present
T4294 5273-5275 IN denotes at
T4295 5276-5280 DT denotes this
T4296 5281-5286 NN denotes stage
T4297 5287-5289 IN denotes of
T4298 5290-5299 NN denotes gestation
T4299 5299-5300 . denotes .
T4300 5300-5423 sentence denotes This demonstrates unequivocally that m-calpain and μ-calpain have distinct physiological roles during early embryogenesis.
T4301 5301-5305 DT denotes This
T4302 5306-5318 VBZ denotes demonstrates
T4303 5319-5332 RB denotes unequivocally
T4304 5333-5337 IN denotes that
T4305 5362-5366 VBP denotes have
T4306 5338-5339 NN denotes m
T4307 5340-5347 NN denotes calpain
T4308 5339-5340 HYPH denotes -
T4309 5348-5351 CC denotes and
T4310 5352-5353 NN denotes μ
T4311 5354-5361 NN denotes calpain
T4312 5353-5354 HYPH denotes -
T4313 5367-5375 JJ denotes distinct
T4314 5390-5395 NNS denotes roles
T4315 5376-5389 JJ denotes physiological
T4316 5396-5402 IN denotes during
T4317 5403-5408 JJ denotes early
T4318 5409-5422 NN denotes embryogenesis
T4319 5422-5423 . denotes .
T4832 5434-5443 NN denotes Isolation
T4833 5444-5447 CC denotes and
T4834 5448-5464 NN denotes characterization
T4835 5465-5467 IN denotes of
T4836 5468-5473 NN denotes Capn2
T4837 5474-5482 VBN denotes targeted
T4838 5491-5497 NNS denotes clones
T4839 5483-5485 NN denotes ES
T4840 5486-5490 NN denotes cell
T4841 5497-5621 sentence denotes Two independent Capn2+/- ES cell lines, designated ES27 and ES36, were isolated from a screen of 305 drug-resistant clones.
T4842 5498-5501 CD denotes Two
T4843 5531-5536 NNS denotes lines
T4844 5502-5513 JJ denotes independent
T4845 5514-5519 NN denotes Capn2
T4846 5519-5520 SYM denotes +
T4847 5520-5521 HYPH denotes /
T4848 5521-5522 SYM denotes -
T4849 5523-5525 NN denotes ES
T4850 5526-5530 NN denotes cell
T4851 5569-5577 VBN denotes isolated
T4852 5536-5538 , denotes ,
T4853 5538-5548 VBN denotes designated
T4854 5549-5553 NN denotes ES27
T4855 5554-5557 CC denotes and
T4856 5558-5562 NN denotes ES36
T4857 5562-5564 , denotes ,
T4858 5564-5568 VBD denotes were
T4859 5578-5582 IN denotes from
T4860 5583-5584 DT denotes a
T4861 5585-5591 NN denotes screen
T4862 5592-5594 IN denotes of
T4863 5595-5598 CD denotes 305
T4864 5614-5620 NNS denotes clones
T4865 5599-5603 NN denotes drug
T4866 5604-5613 JJ denotes resistant
T4867 5603-5604 HYPH denotes -
T4868 5620-5621 . denotes .
T4869 5621-5728 sentence denotes Correct targeting of the Capn2 locus was established both by Southern blot hybridization and PCR analysis.
T4870 5622-5629 JJ denotes Correct
T4871 5630-5639 NN denotes targeting
T4872 5663-5674 VBN denotes established
T4873 5640-5642 IN denotes of
T4874 5643-5646 DT denotes the
T4875 5653-5658 NN denotes locus
T4876 5647-5652 NN denotes Capn2
T4877 5659-5662 VBD denotes was
T4878 5675-5679 CC denotes both
T4879 5680-5682 IN denotes by
T4880 5683-5691 NNP denotes Southern
T4881 5692-5696 NN denotes blot
T4882 5697-5710 NN denotes hybridization
T4883 5711-5714 CC denotes and
T4884 5715-5718 NN denotes PCR
T4885 5719-5727 NN denotes analysis
T4886 5727-5728 . denotes .
T4887 5728-5942 sentence denotes A probe located outside the short (upstream) arm of homology hybridized to a 3.5-kb BamHI fragment of the wild-type allele and 5.3-kb BamHI fragment of the mutant allele as predicted from genomic maps (Figure 2A).
T4888 5729-5730 DT denotes A
T4889 5731-5736 NN denotes probe
T4890 5790-5800 VBN denotes hybridized
T4891 5737-5744 VBN denotes located
T4892 5745-5752 RB denotes outside
T4893 5753-5756 DT denotes the
T4894 5774-5777 NN denotes arm
T4895 5757-5762 JJ denotes short
T4896 5763-5764 -LRB- denotes (
T4897 5764-5772 JJ denotes upstream
T4898 5772-5773 -RRB- denotes )
T4899 5778-5780 IN denotes of
T4900 5781-5789 NN denotes homology
T4901 5801-5803 IN denotes to
T4902 5804-5805 DT denotes a
T4903 5819-5827 NN denotes fragment
T4904 5806-5809 CD denotes 3.5
T4905 5810-5812 NN denotes kb
T4906 5809-5810 HYPH denotes -
T4907 5813-5818 NN denotes BamHI
T4908 5828-5830 IN denotes of
T4909 5831-5834 DT denotes the
T4910 5845-5851 NN denotes allele
T4911 5835-5839 JJ denotes wild
T4912 5840-5844 NN denotes type
T4913 5839-5840 HYPH denotes -
T4914 5852-5855 CC denotes and
T4915 5856-5859 CD denotes 5.3
T4916 5860-5862 NN denotes kb
T4917 5859-5860 HYPH denotes -
T4918 5869-5877 NN denotes fragment
T4919 5863-5868 NN denotes BamHI
T4920 5878-5880 IN denotes of
T4921 5881-5884 DT denotes the
T4922 5892-5898 NN denotes allele
T4923 5885-5891 NN denotes mutant
T4924 5899-5901 IN denotes as
T4925 5902-5911 VBN denotes predicted
T4926 5912-5916 IN denotes from
T4927 5917-5924 JJ denotes genomic
T4928 5925-5929 NNS denotes maps
T4929 5930-5931 -LRB- denotes (
T4930 5938-5940 NN denotes 2A
T4931 5931-5937 NN denotes Figure
T4932 5940-5941 -RRB- denotes )
T4933 5941-5942 . denotes .
T4934 5942-6167 sentence denotes The same probe also detected the expected 7.2-kb wild-type and 6.4-kb mutant BglII fragments, 4.9-kb wild-type and 5.7-kb mutant NcoI fragments, as well as 7.2-kb wild-type and 4.9-kb mutant BglII/AgeI fragments (not shown).
T4935 5943-5946 DT denotes The
T4936 5952-5957 NN denotes probe
T4937 5947-5951 JJ denotes same
T4938 5963-5971 VBD denotes detected
T4939 5958-5962 RB denotes also
T4940 5972-5975 DT denotes the
T4941 6026-6035 NNS denotes fragments
T4942 5976-5984 VBN denotes expected
T4943 5985-5988 CD denotes 7.2
T4944 5989-5991 NN denotes kb
T4945 5988-5989 HYPH denotes -
T4946 5992-5996 JJ denotes wild
T4947 5997-6001 NN denotes type
T4948 5996-5997 HYPH denotes -
T4949 6002-6005 CC denotes and
T4950 6006-6009 CD denotes 6.4
T4951 6010-6012 NN denotes kb
T4952 6009-6010 HYPH denotes -
T4953 6013-6019 NN denotes mutant
T4954 6020-6025 NN denotes BglII
T4955 6035-6037 , denotes ,
T4956 6037-6040 CD denotes 4.9
T4957 6041-6043 NN denotes kb
T4958 6040-6041 HYPH denotes -
T4959 6077-6086 NNS denotes fragments
T4960 6044-6048 JJ denotes wild
T4961 6049-6053 NN denotes type
T4962 6048-6049 HYPH denotes -
T4963 6054-6057 CC denotes and
T4964 6058-6061 CD denotes 5.7
T4965 6062-6064 NN denotes kb
T4966 6061-6062 HYPH denotes -
T4967 6065-6071 NN denotes mutant
T4968 6072-6076 NN denotes NcoI
T4969 6086-6088 , denotes ,
T4970 6088-6090 RB denotes as
T4971 6096-6098 IN denotes as
T4972 6091-6095 RB denotes well
T4973 6099-6102 CD denotes 7.2
T4974 6103-6105 NN denotes kb
T4975 6102-6103 HYPH denotes -
T4976 6145-6154 NNS denotes fragments
T4977 6106-6110 JJ denotes wild
T4978 6111-6115 NN denotes type
T4979 6110-6111 HYPH denotes -
T4980 6116-6119 CC denotes and
T4981 6120-6123 CD denotes 4.9
T4982 6124-6126 NN denotes kb
T4983 6123-6124 HYPH denotes -
T4984 6127-6133 NN denotes mutant
T4985 6134-6139 NN denotes BglII
T4986 6140-6144 NN denotes AgeI
T4987 6139-6140 HYPH denotes /
T4988 6155-6156 -LRB- denotes (
T4989 6160-6165 VBN denotes shown
T4990 6156-6159 RB denotes not
T4991 6165-6166 -RRB- denotes )
T4992 6166-6167 . denotes .
T4993 6167-6364 sentence denotes A probe derived from the PGK-Neo cassette recognized only the 5.3-kb BamHI fragment in Capn2+/- ES cells, suggesting that the targeting vector had integrated solely at the Capn2 locus (not shown).
T4994 6168-6169 DT denotes A
T4995 6170-6175 NN denotes probe
T4996 6210-6220 VBD denotes recognized
T4997 6176-6183 VBN denotes derived
T4998 6184-6188 IN denotes from
T4999 6189-6192 DT denotes the
T5000 6201-6209 NN denotes cassette
T5001 6193-6196 NN denotes PGK
T5002 6197-6200 NN denotes Neo
T5003 6196-6197 HYPH denotes -
T5004 6221-6225 RB denotes only
T5005 6243-6251 NN denotes fragment
T5006 6226-6229 DT denotes the
T5007 6230-6233 CD denotes 5.3
T5008 6234-6236 NN denotes kb
T5009 6233-6234 HYPH denotes -
T5010 6237-6242 NN denotes BamHI
T5011 6252-6254 IN denotes in
T5012 6255-6260 NN denotes Capn2
T5013 6264-6266 NN denotes ES
T5014 6260-6261 SYM denotes +
T5015 6261-6262 HYPH denotes /
T5016 6262-6263 SYM denotes -
T5017 6267-6272 NNS denotes cells
T5018 6272-6274 , denotes ,
T5019 6274-6284 VBG denotes suggesting
T5020 6285-6289 IN denotes that
T5021 6315-6325 VBN denotes integrated
T5022 6290-6293 DT denotes the
T5023 6304-6310 NN denotes vector
T5024 6294-6303 NN denotes targeting
T5025 6311-6314 VBD denotes had
T5026 6326-6332 RB denotes solely
T5027 6333-6335 IN denotes at
T5028 6336-6339 DT denotes the
T5029 6346-6351 NN denotes locus
T5030 6340-6345 NN denotes Capn2
T5031 6352-6353 -LRB- denotes (
T5032 6357-6362 VBN denotes shown
T5033 6353-6356 RB denotes not
T5034 6362-6363 -RRB- denotes )
T5035 6363-6364 . denotes .
T5036 6364-6502 sentence denotes A PCR screening method was also established that generated a wild-type product of 2,749 bp and a 2,711 bp product from the mutant allele.
T5037 6365-6366 DT denotes A
T5038 6381-6387 NN denotes method
T5039 6367-6370 NN denotes PCR
T5040 6371-6380 NN denotes screening
T5041 6397-6408 VBN denotes established
T5042 6388-6391 VBD denotes was
T5043 6392-6396 RB denotes also
T5044 6409-6413 WDT denotes that
T5045 6414-6423 VBD denotes generated
T5046 6424-6425 DT denotes a
T5047 6436-6443 NN denotes product
T5048 6426-6430 JJ denotes wild
T5049 6431-6435 NN denotes type
T5050 6430-6431 HYPH denotes -
T5051 6444-6446 IN denotes of
T5052 6447-6452 CD denotes 2,749
T5053 6453-6455 NN denotes bp
T5054 6456-6459 CC denotes and
T5055 6460-6461 DT denotes a
T5056 6471-6478 NN denotes product
T5057 6462-6467 CD denotes 2,711
T5058 6468-6470 NN denotes bp
T5059 6479-6483 IN denotes from
T5060 6484-6487 DT denotes the
T5061 6495-6501 NN denotes allele
T5062 6488-6494 NN denotes mutant
T5063 6501-6502 . denotes .
T5064 6502-6584 sentence denotes The 2,711 bp product was only evident in the two targeted cell lines (Figure 2B).
T5065 6503-6506 DT denotes The
T5066 6516-6523 NN denotes product
T5067 6507-6512 CD denotes 2,711
T5068 6513-6515 NN denotes bp
T5069 6524-6527 VBD denotes was
T5070 6528-6532 RB denotes only
T5071 6533-6540 JJ denotes evident
T5072 6541-6543 IN denotes in
T5073 6544-6547 DT denotes the
T5074 6566-6571 NNS denotes lines
T5075 6548-6551 CD denotes two
T5076 6552-6560 VBN denotes targeted
T5077 6561-6565 NN denotes cell
T5078 6572-6573 -LRB- denotes (
T5079 6580-6582 NN denotes 2B
T5080 6573-6579 NN denotes Figure
T5081 6582-6583 -RRB- denotes )
T5082 6583-6584 . denotes .
T6635 7880-7890 NN denotes Generation
T6636 7891-7893 IN denotes of
T6637 7894-7902 JJ denotes chimeric
T6638 7903-7907 NNS denotes mice
T6639 7908-7911 CC denotes and
T6640 7912-7920 NN denotes germline
T6641 7921-7933 NN denotes transmission
T6642 7934-7936 IN denotes of
T6643 7937-7940 DT denotes the
T6644 7954-7960 NN denotes allele
T6645 7941-7947 NN denotes mutant
T6646 7948-7953 NN denotes Capn2
T6647 7960-8068 sentence denotes Eight chimeric male mice were produced in morula aggregation experiments using the Capn2+/- ES27 cell line.
T6648 7961-7966 CD denotes Eight
T6649 7981-7985 NNS denotes mice
T6650 7967-7975 JJ denotes chimeric
T6651 7976-7980 JJ denotes male
T6652 7991-7999 VBN denotes produced
T6653 7986-7990 VBD denotes were
T6654 8000-8002 IN denotes in
T6655 8003-8009 NN denotes morula
T6656 8010-8021 NN denotes aggregation
T6657 8022-8033 NNS denotes experiments
T6658 8034-8039 VBG denotes using
T6659 8040-8043 DT denotes the
T6660 8063-8067 NN denotes line
T6661 8044-8049 NN denotes Capn2
T6662 8049-8050 SYM denotes +
T6663 8050-8051 HYPH denotes /
T6664 8051-8052 SYM denotes -
T6665 8053-8057 NN denotes ES27
T6666 8058-8062 NN denotes cell
T6667 8067-8068 . denotes .
T6668 8068-8171 sentence denotes Two of these males transmitted the Capn2+/- ES27 genotype through the germline into the F1 generation.
T6669 8069-8072 CD denotes Two
T6670 8088-8099 VBD denotes transmitted
T6671 8073-8075 IN denotes of
T6672 8076-8081 DT denotes these
T6673 8082-8087 NNS denotes males
T6674 8100-8103 DT denotes the
T6675 8118-8126 NN denotes genotype
T6676 8104-8109 NN denotes Capn2
T6677 8109-8110 SYM denotes +
T6678 8110-8111 HYPH denotes /
T6679 8111-8112 SYM denotes -
T6680 8113-8117 NN denotes ES27
T6681 8127-8134 IN denotes through
T6682 8135-8138 DT denotes the
T6683 8139-8147 NN denotes germline
T6684 8148-8152 IN denotes into
T6685 8153-8156 DT denotes the
T6686 8160-8170 NN denotes generation
T6687 8157-8159 NN denotes F1
T6688 8170-8171 . denotes .
T6689 8171-8288 sentence denotes Heterozygous Capn2+/- animals appeared normal, with no obvious defects in gross anatomy, reproduction, or life span.
T6690 8172-8184 JJ denotes Heterozygous
T6691 8194-8201 NNS denotes animals
T6692 8185-8190 NN denotes Capn2
T6693 8190-8191 SYM denotes +
T6694 8191-8192 HYPH denotes /
T6695 8192-8193 SYM denotes -
T6696 8202-8210 VBD denotes appeared
T6697 8211-8217 JJ denotes normal
T6698 8217-8219 , denotes ,
T6699 8219-8223 IN denotes with
T6700 8224-8226 DT denotes no
T6701 8235-8242 NNS denotes defects
T6702 8227-8234 JJ denotes obvious
T6703 8243-8245 IN denotes in
T6704 8246-8251 JJ denotes gross
T6705 8252-8259 NN denotes anatomy
T6706 8259-8261 , denotes ,
T6707 8261-8273 NN denotes reproduction
T6708 8273-8275 , denotes ,
T6709 8275-8277 CC denotes or
T6710 8278-8282 NN denotes life
T6711 8283-8287 NN denotes span
T6712 8287-8288 . denotes .
T6713 8288-8386 sentence denotes Out of 199 weanlings from heterozygous intercrosses, no Capn2-/- progeny were detected (Table 2).
T6714 8289-8292 IN denotes Out
T6715 8367-8375 VBN denotes detected
T6716 8293-8295 IN denotes of
T6717 8296-8299 CD denotes 199
T6718 8300-8309 NNS denotes weanlings
T6719 8310-8314 IN denotes from
T6720 8315-8327 JJ denotes heterozygous
T6721 8328-8340 NNS denotes intercrosses
T6722 8340-8342 , denotes ,
T6723 8342-8344 DT denotes no
T6724 8354-8361 NN denotes progeny
T6725 8345-8350 NN denotes Capn2
T6726 8350-8351 SYM denotes -
T6727 8351-8352 HYPH denotes /
T6728 8352-8353 SYM denotes -
T6729 8362-8366 VBD denotes were
T6730 8376-8377 -LRB- denotes (
T6731 8377-8382 NN denotes Table
T6732 8383-8384 CD denotes 2
T6733 8384-8385 -RRB- denotes )
T6734 8385-8386 . denotes .
T6735 8386-8478 sentence denotes We did not observe high rates of perinatal death, and no Capn2-/- stillborns were observed.
T6736 8387-8389 PRP denotes We
T6737 8398-8405 VB denotes observe
T6738 8390-8393 VBD denotes did
T6739 8394-8397 RB denotes not
T6740 8406-8410 JJ denotes high
T6741 8411-8416 NNS denotes rates
T6742 8417-8419 IN denotes of
T6743 8420-8429 JJ denotes perinatal
T6744 8430-8435 NN denotes death
T6745 8435-8437 , denotes ,
T6746 8437-8440 CC denotes and
T6747 8441-8443 DT denotes no
T6748 8453-8463 NNS denotes stillborns
T6749 8444-8449 NN denotes Capn2
T6750 8449-8450 SYM denotes -
T6751 8450-8451 HYPH denotes /
T6752 8451-8452 SYM denotes -
T6753 8469-8477 VBN denotes observed
T6754 8464-8468 VBD denotes were
T6755 8477-8478 . denotes .
T6756 8478-8568 sentence denotes This suggested that Capn2-/- animals perished at some stage during embryonic development.
T6757 8479-8483 DT denotes This
T6758 8484-8493 VBD denotes suggested
T6759 8494-8498 IN denotes that
T6760 8516-8524 VBN denotes perished
T6761 8499-8504 NN denotes Capn2
T6762 8508-8515 NNS denotes animals
T6763 8504-8505 SYM denotes -
T6764 8505-8506 HYPH denotes /
T6765 8506-8507 SYM denotes -
T6766 8525-8527 IN denotes at
T6767 8528-8532 DT denotes some
T6768 8533-8538 NN denotes stage
T6769 8539-8545 IN denotes during
T6770 8546-8555 JJ denotes embryonic
T6771 8556-8567 NN denotes development
T6772 8567-8568 . denotes .
T6773 8568-8735 sentence denotes In an attempt to determine if embryonic death occurred at a post-implantation stage, embryos were harvested for genotyping at different times between E10.5 and E18.5.
T6774 8569-8571 IN denotes In
T6775 8667-8676 VBN denotes harvested
T6776 8572-8574 DT denotes an
T6777 8575-8582 NN denotes attempt
T6778 8583-8585 TO denotes to
T6779 8586-8595 VB denotes determine
T6780 8596-8598 IN denotes if
T6781 8615-8623 VBD denotes occurred
T6782 8599-8608 JJ denotes embryonic
T6783 8609-8614 NN denotes death
T6784 8624-8626 IN denotes at
T6785 8627-8628 DT denotes a
T6786 8647-8652 NN denotes stage
T6787 8629-8646 JJ denotes post-implantation
T6788 8652-8654 , denotes ,
T6789 8654-8661 NNS denotes embryos
T6790 8662-8666 VBD denotes were
T6791 8677-8680 IN denotes for
T6792 8681-8691 NN denotes genotyping
T6793 8692-8694 IN denotes at
T6794 8695-8704 JJ denotes different
T6795 8705-8710 NNS denotes times
T6796 8711-8718 IN denotes between
T6797 8719-8724 NN denotes E10.5
T6798 8725-8728 CC denotes and
T6799 8729-8734 NN denotes E18.5
T6800 8734-8735 . denotes .
T6801 8735-8828 sentence denotes No Capn2-/- embryos were observed and no signs of embryo resorption were detected (Table 2).
T6802 8736-8738 DT denotes No
T6803 8748-8755 NNS denotes embryos
T6804 8739-8744 NN denotes Capn2
T6805 8744-8745 SYM denotes -
T6806 8745-8746 HYPH denotes /
T6807 8746-8747 SYM denotes -
T6808 8761-8769 VBN denotes observed
T6809 8756-8760 VBD denotes were
T6810 8770-8773 CC denotes and
T6811 8774-8776 DT denotes no
T6812 8777-8782 NNS denotes signs
T6813 8809-8817 VBN denotes detected
T6814 8783-8785 IN denotes of
T6815 8786-8792 NN denotes embryo
T6816 8793-8803 NN denotes resorption
T6817 8804-8808 VBD denotes were
T6818 8818-8819 -LRB- denotes (
T6819 8819-8824 NN denotes Table
T6820 8825-8826 CD denotes 2
T6821 8826-8827 -RRB- denotes )
T6822 8827-8828 . denotes .
T6823 8828-8907 sentence denotes This indicated that the Capn2-/- embryos might be dying prior to implantation.
T6824 8829-8833 DT denotes This
T6825 8834-8843 VBD denotes indicated
T6826 8844-8848 IN denotes that
T6827 8879-8884 VBG denotes dying
T6828 8849-8852 DT denotes the
T6829 8862-8869 NNS denotes embryos
T6830 8853-8858 NN denotes Capn2
T6831 8858-8859 SYM denotes -
T6832 8859-8860 HYPH denotes /
T6833 8860-8861 SYM denotes -
T6834 8870-8875 MD denotes might
T6835 8876-8878 VB denotes be
T6836 8885-8890 JJ denotes prior
T6837 8891-8893 IN denotes to
T6838 8894-8906 NN denotes implantation
T6839 8906-8907 . denotes .
T6840 8907-9048 sentence denotes Embryos were then flushed from the oviducts of pregnant females at E2.5 or E3.5, and genotyped by means of a nested PCR strategy (Figure 4).
T6841 8908-8915 NNS denotes Embryos
T6842 8926-8933 VBN denotes flushed
T6843 8916-8920 VBD denotes were
T6844 8921-8925 RB denotes then
T6845 8934-8938 IN denotes from
T6846 8939-8942 DT denotes the
T6847 8943-8951 NNS denotes oviducts
T6848 8952-8954 IN denotes of
T6849 8955-8963 JJ denotes pregnant
T6850 8964-8971 NNS denotes females
T6851 8972-8974 IN denotes at
T6852 8975-8979 NN denotes E2.5
T6853 8980-8982 CC denotes or
T6854 8983-8987 NN denotes E3.5
T6855 8987-8989 , denotes ,
T6856 8989-8992 CC denotes and
T6857 8993-9002 VBN denotes genotyped
T6858 9003-9005 IN denotes by
T6859 9006-9011 NNS denotes means
T6860 9012-9014 IN denotes of
T6861 9015-9016 DT denotes a
T6862 9028-9036 NN denotes strategy
T6863 9017-9023 VBN denotes nested
T6864 9024-9027 NN denotes PCR
T6865 9037-9038 -LRB- denotes (
T6866 9038-9044 NN denotes Figure
T6867 9045-9046 CD denotes 4
T6868 9046-9047 -RRB- denotes )
T6869 9047-9048 . denotes .
T6870 9048-9142 sentence denotes Two of 90 successfully genotyped pre-implantation embryos were Capn2-/-, (Table 2; Figure 5).
T6871 9049-9052 CD denotes Two
T6872 9107-9111 VBD denotes were
T6873 9053-9055 IN denotes of
T6874 9056-9058 CD denotes 90
T6875 9099-9106 NNS denotes embryos
T6876 9059-9071 RB denotes successfully
T6877 9072-9081 VBN denotes genotyped
T6878 9082-9098 JJ denotes pre-implantation
T6879 9112-9117 NN denotes Capn2
T6880 9117-9118 SYM denotes -
T6881 9118-9119 HYPH denotes /
T6882 9119-9120 SYM denotes -
T6883 9120-9122 , denotes ,
T6884 9122-9123 -LRB- denotes (
T6885 9132-9138 NN denotes Figure
T6886 9123-9128 NN denotes Table
T6887 9129-9130 CD denotes 2
T6888 9130-9131 : denotes ;
T6889 9139-9140 CD denotes 5
T6890 9140-9141 -RRB- denotes )
T6891 9141-9142 . denotes .
T6892 9142-9262 sentence denotes Both of these Capn2-/- embryos were isolated at the 8-cell stage and did not display any obvious morphological defects.
T6893 9143-9147 DT denotes Both
T6894 9179-9187 VBN denotes isolated
T6895 9148-9150 IN denotes of
T6896 9151-9156 DT denotes these
T6897 9166-9173 NNS denotes embryos
T6898 9157-9162 NN denotes Capn2
T6899 9162-9163 SYM denotes -
T6900 9163-9164 HYPH denotes /
T6901 9164-9165 SYM denotes -
T6902 9174-9178 VBD denotes were
T6903 9188-9190 IN denotes at
T6904 9191-9194 DT denotes the
T6905 9202-9207 NN denotes stage
T6906 9195-9196 CD denotes 8
T6907 9197-9201 NN denotes cell
T6908 9196-9197 HYPH denotes -
T6909 9208-9211 CC denotes and
T6910 9212-9215 VBD denotes did
T6911 9220-9227 VB denotes display
T6912 9216-9219 RB denotes not
T6913 9228-9231 DT denotes any
T6914 9254-9261 NNS denotes defects
T6915 9232-9239 JJ denotes obvious
T6916 9240-9253 JJ denotes morphological
T6917 9261-9262 . denotes .
T6918 9262-9341 sentence denotes None of the 46 successfully genotyped blastocyst-staged embryos were Capn2-/-.
T6919 9263-9267 NN denotes None
T6920 9327-9331 VBD denotes were
T6921 9268-9270 IN denotes of
T6922 9271-9274 DT denotes the
T6923 9319-9326 NNS denotes embryos
T6924 9275-9277 CD denotes 46
T6925 9278-9290 RB denotes successfully
T6926 9291-9300 VBN denotes genotyped
T6927 9301-9311 NN denotes blastocyst
T6928 9312-9318 VBN denotes staged
T6929 9311-9312 HYPH denotes -
T6930 9332-9337 NN denotes Capn2
T6931 9337-9338 SYM denotes -
T6932 9338-9339 HYPH denotes /
T6933 9339-9340 SYM denotes -
T6934 9340-9341 . denotes .
T6935 9341-9513 sentence denotes The scarcity of Capn2-deficient embryos surviving to the 8-cell stage suggested that the loss of m-calpain activity must fatally compromise the viability of early embryos.
T6936 9342-9345 DT denotes The
T6937 9346-9354 NN denotes scarcity
T6938 9412-9421 VBD denotes suggested
T6939 9355-9357 IN denotes of
T6940 9358-9363 NN denotes Capn2
T6941 9364-9373 JJ denotes deficient
T6942 9363-9364 HYPH denotes -
T6943 9374-9381 NNS denotes embryos
T6944 9382-9391 VBG denotes surviving
T6945 9392-9394 IN denotes to
T6946 9395-9398 DT denotes the
T6947 9406-9411 NN denotes stage
T6948 9399-9400 CD denotes 8
T6949 9401-9405 NN denotes cell
T6950 9400-9401 HYPH denotes -
T6951 9422-9426 IN denotes that
T6952 9471-9481 VB denotes compromise
T6953 9427-9430 DT denotes the
T6954 9431-9435 NN denotes loss
T6955 9436-9438 IN denotes of
T6956 9439-9440 NN denotes m
T6957 9441-9448 NN denotes calpain
T6958 9440-9441 HYPH denotes -
T6959 9449-9457 NN denotes activity
T6960 9458-9462 MD denotes must
T6961 9463-9470 RB denotes fatally
T6962 9482-9485 DT denotes the
T6963 9486-9495 NN denotes viability
T6964 9496-9498 IN denotes of
T6965 9499-9504 JJ denotes early
T6966 9505-9512 NNS denotes embryos
T6967 9512-9513 . denotes .
T6968 9513-9698 sentence denotes Furthermore, it is possible that persistence of some maternally derived mRNA transcript or protein might have allowed a small number of Capn2-/- embryos to survive to the morula-stage.
T6969 9514-9525 RB denotes Furthermore
T6970 9530-9532 VBZ denotes is
T6971 9525-9527 , denotes ,
T6972 9527-9529 PRP denotes it
T6973 9533-9541 JJ denotes possible
T6974 9542-9546 IN denotes that
T6975 9624-9631 VBN denotes allowed
T6976 9547-9558 NN denotes persistence
T6977 9559-9561 IN denotes of
T6978 9562-9566 DT denotes some
T6979 9591-9601 NN denotes transcript
T6980 9567-9577 RB denotes maternally
T6981 9578-9585 VBN denotes derived
T6982 9586-9590 NN denotes mRNA
T6983 9602-9604 CC denotes or
T6984 9605-9612 NN denotes protein
T6985 9613-9618 MD denotes might
T7359 14138-14139 . denotes .
T15181 27201-27202 . denotes .
T15180 27197-27201 DT denotes this
T15179 27193-27196 IN denotes for
T15178 27175-27186 JJ denotes mechanistic
T15177 27187-27192 NN denotes basis
T15176 27173-27174 DT denotes a
T15175 27161-27165 MD denotes will
T15174 27153-27160 NNS denotes studies
T15173 27146-27152 JJ denotes future
T15172 27166-27172 VB denotes reveal
T15171 27138-27145 RB denotes Perhaps
T15170 27137-27202 sentence denotes Perhaps future studies will reveal a mechanistic basis for this.
T15169 27136-27137 . denotes .
T15160 27060-27067 VBP denotes suggest
T15159 27047-27059 NNS denotes observations
T15158 27041-27046 DT denotes These
T15157 27040-27137 sentence denotes These observations suggest a developmental advantage associated with reduced calpain expression.
T15156 27039-27040 . denotes .
T15155 27038-27039 -RRB- denotes ]
T15154 27036-27038 CD denotes 32
T15153 27035-27036 -LRB- denotes [
T15152 27013-27017 JJ denotes null
T15151 27002-27012 JJ denotes homozygous
T15150 27018-27025 NN denotes progeny
T15149 26999-27001 DT denotes no
T15148 27026-27034 VBN denotes observed
T15147 26994-26998 IN denotes with
T15146 26992-26994 , denotes ,
T15145 26980-26992 JJ denotes heterozygous
T15144 26975-26979 VBD denotes were
T15143 26973-26974 NN denotes %
T15142 26969-26973 CD denotes 77.5
T15141 26965-26968 CC denotes and
T15140 26959-26960 HYPH denotes -
T15139 26960-26964 NN denotes type
T15138 26955-26959 JJ denotes wild
T15168 27118-27125 NN denotes calpain
T15167 27126-27136 NN denotes expression
T15166 27110-27117 VBN denotes reduced
T15165 27105-27109 IN denotes with
T15164 27094-27104 VBN denotes associated
T15163 27070-27083 JJ denotes developmental
T15162 27084-27093 NN denotes advantage
T15448 27333-27340 NN denotes calpain
T15447 27322-27332 JJ denotes ubiquitous
T15446 27318-27321 CD denotes two
T15445 27341-27349 NNS denotes isoforms
T15444 27314-27317 DT denotes the
T15443 27311-27313 IN denotes of
T15442 27291-27304 JJ denotes physiological
T15441 27305-27310 NNS denotes roles
T15440 27287-27290 DT denotes the
T15439 27277-27286 VBG denotes regarding
T15438 27257-27266 JJ denotes important
T15437 27267-27276 NNS denotes questions
T15436 27253-27256 CD denotes two
T15435 27239-27242 VBZ denotes has
T15434 27234-27238 RB denotes here
T15433 27224-27233 VBN denotes presented
T15432 27243-27252 VBN denotes clarified
T15431 27219-27223 NN denotes work
T15430 27215-27218 DT denotes The
T15552 27899-27900 . denotes .
T16931 30007-30008 . denotes .
T16930 29996-29997 -RRB- denotes )
T16929 29994-29996 NN denotes tk
T16928 29993-29994 -LRB- denotes (
T16927 29986-29992 NN denotes kinase
T16926 29976-29985 NN denotes thymidine
T16925 29972-29975 CC denotes and
T16924 29967-29968 HYPH denotes -
T16923 29968-29971 NN denotes Neo
T16922 29964-29967 NN denotes PGK
T16921 29998-30007 NNS denotes cassettes
T16920 29960-29963 DT denotes the
T16919 29952-29959 IN denotes between
T16918 29943-29951 VBN denotes inserted
T16917 29941-29943 , denotes ,
T16916 29939-29941 CD denotes 12
T16915 29932-29938 NN denotes intron
T16914 29929-29931 IN denotes to
T16913 29927-29928 CD denotes 7
T16912 29920-29926 NN denotes intron
T16911 29915-29919 IN denotes from
T16910 29905-29914 VBG denotes extending
T16909 29903-29905 , denotes ,
T16908 29889-29890 HYPH denotes -
T16907 29890-29894 NN denotes KpnI
T16906 29882-29889 NN denotes HindIII
T16905 29878-29879 HYPH denotes -
T16904 29879-29881 NN denotes kb
T17711 32278-32279 . denotes .
T20608 38012-38013 . denotes .
T20607 38002-38012 NN denotes genotyping
T20606 37998-38001 NN denotes PCR
T20605 37994-37997 IN denotes for
T20604 37984-37988 RB denotes then
T20603 37979-37983 VBD denotes were
T20602 37989-37993 VBN denotes used
T20601 37971-37978 NNS denotes Lysates
T20600 37970-38013 sentence denotes Lysates were then used for PCR genotyping.
T20599 37969-37970 . denotes .
T20598 37957-37967 NN denotes proteinase
T20597 37968-37969 NN denotes K
T20596 37953-37956 DT denotes the
T20595 37942-37952 VB denotes inactivate
T20594 37939-37941 TO denotes to
T20593 37936-37938 NN denotes °C
T20592 37934-37936 CD denotes 95
T20591 37931-37933 IN denotes at
T20590 37923-37930 NNS denotes minutes
T20589 37919-37922 CD denotes ten
T20588 37916-37918 IN denotes by
T20587 37907-37915 VBN denotes followed
T20586 37905-37907 , denotes ,
T20585 37904-37905 -RRB- denotes )
T20584 37901-37904 CD denotes 8.0
T20583 37896-37898 , denotes ,
T20582 37884-37894 NN denotes proteinase
T20581 37881-37883 NN denotes mL
T20580 37880-37881 SYM denotes /
T20579 37895-37896 NN denotes K
T20578 37878-37880 NN denotes μL
T20577 37874-37877 CD denotes 200
T21151 6595-6611 NN denotes Characterization
T21152 6612-6614 IN denotes of
T21153 6615-6623 VBN denotes targeted
T21154 6632-6637 NNS denotes lines
T21155 6624-6626 NN denotes ES
T21156 6627-6631 NN denotes cell
T21157 6637-6638 . denotes .
T21158 6638-6726 sentence denotes (A) Targeted disruption of the Capn2 locus was detected initially by Southern blotting.
T21159 6639-6640 -LRB- denotes (
T21160 6640-6641 LS denotes A
T21161 6686-6694 VBN denotes detected
T21162 6641-6642 -RRB- denotes )
T21163 6643-6651 VBN denotes Targeted
T21164 6652-6662 NN denotes disruption
T21165 6663-6665 IN denotes of
T21166 6666-6669 DT denotes the
T21167 6676-6681 NN denotes locus
T21168 6670-6675 NN denotes Capn2
T21169 6682-6685 VBD denotes was
T21170 6695-6704 RB denotes initially
T21171 6705-6707 IN denotes by
T21172 6708-6716 NNP denotes Southern
T21173 6717-6725 NN denotes blotting
T21174 6725-6726 . denotes .
T21175 6726-6951 sentence denotes Membranes were blotted with BamHI-digested genomic DNA extracted from ES cells and hybridized with a DIG-labeled 823 bp BamHI/HindIII fragment located immediately upstream of the short arm of the targeting vector (Figure 1).
T21176 6727-6736 NNS denotes Membranes
T21177 6742-6749 VBN denotes blotted
T21178 6737-6741 VBD denotes were
T21179 6750-6754 IN denotes with
T21180 6755-6760 NN denotes BamHI
T21181 6761-6769 VBN denotes digested
T21182 6760-6761 HYPH denotes -
T21183 6778-6781 NN denotes DNA
T21184 6770-6777 JJ denotes genomic
T21185 6782-6791 VBN denotes extracted
T21186 6792-6796 IN denotes from
T21187 6797-6799 NN denotes ES
T21188 6800-6805 NNS denotes cells
T21189 6806-6809 CC denotes and
T21190 6810-6820 VBD denotes hybridized
T21191 6821-6825 IN denotes with
T21192 6826-6827 DT denotes a
T21193 6861-6869 NN denotes fragment
T21194 6828-6831 NN denotes DIG
T21195 6832-6839 VBN denotes labeled
T21196 6831-6832 HYPH denotes -
T21197 6840-6843 CD denotes 823
T21198 6844-6846 NN denotes bp
T21199 6847-6852 NN denotes BamHI
T21200 6853-6860 NN denotes HindIII
T21201 6852-6853 HYPH denotes /
T21202 6870-6877 VBN denotes located
T21203 6878-6889 RB denotes immediately
T21204 6890-6898 IN denotes upstream
T21205 6899-6901 IN denotes of
T21206 6902-6905 DT denotes the
T21207 6912-6915 NN denotes arm
T21208 6906-6911 JJ denotes short
T21209 6916-6918 IN denotes of
T21210 6919-6922 DT denotes the
T21211 6933-6939 NN denotes vector
T21212 6923-6932 NN denotes targeting
T21213 6940-6941 -LRB- denotes (
T21214 6941-6947 NN denotes Figure
T21215 6948-6949 CD denotes 1
T21216 6949-6950 -RRB- denotes )
T21217 6950-6951 . denotes .
T21218 6951-7155 sentence denotes A 3.5-kb BamHI fragment corresponding to the wild-type allele was present in all cells, whereas a 5.3-kb fragment from the mutant allele was detected in two targeted cell lines, designated ES27 and ES36.
T21219 6952-6953 DT denotes A
T21220 6967-6975 NN denotes fragment
T21221 6954-6957 CD denotes 3.5
T21222 6958-6960 NN denotes kb
T21223 6957-6958 HYPH denotes -
T21224 6961-6966 NN denotes BamHI
T21225 7014-7017 VBD denotes was
T21226 6976-6989 VBG denotes corresponding
T21227 6990-6992 IN denotes to
T21228 6993-6996 DT denotes the
T21229 7007-7013 NN denotes allele
T21230 6997-7001 JJ denotes wild
T21231 7002-7006 NN denotes type
T21232 7001-7002 HYPH denotes -
T21233 7018-7025 JJ denotes present
T21234 7026-7028 IN denotes in
T21235 7029-7032 DT denotes all
T21236 7033-7038 NNS denotes cells
T21237 7038-7040 , denotes ,
T21238 7040-7047 IN denotes whereas
T21239 7093-7101 VBN denotes detected
T21240 7048-7049 DT denotes a
T21241 7057-7065 NN denotes fragment
T21242 7050-7053 CD denotes 5.3
T21243 7054-7056 NN denotes kb
T21244 7053-7054 HYPH denotes -
T21245 7066-7070 IN denotes from
T21246 7071-7074 DT denotes the
T21247 7082-7088 NN denotes allele
T21248 7075-7081 NN denotes mutant
T21249 7089-7092 VBD denotes was
T21250 7102-7104 IN denotes in
T21251 7105-7108 CD denotes two
T21252 7123-7128 NNS denotes lines
T21253 7109-7117 VBN denotes targeted
T21254 7118-7122 NN denotes cell
T21255 7128-7130 , denotes ,
T21256 7130-7140 VBN denotes designated
T21257 7141-7145 NN denotes ES27
T21258 7146-7149 CC denotes and
T21259 7150-7154 NN denotes ES36
T21260 7154-7155 . denotes .
T21261 7155-7336 sentence denotes (B) PCR genotyping was carried out with two separate reactions designed to amplify either a 2,748 bp segment from the wild-type allele or a 2,711 bp segment from the mutant allele.
T21262 7156-7157 -LRB- denotes (
T21263 7157-7158 LS denotes B
T21264 7179-7186 VBN denotes carried
T21265 7158-7159 -RRB- denotes )
T21266 7160-7163 NN denotes PCR
T21267 7164-7174 NN denotes genotyping
T21268 7175-7178 VBD denotes was
T21269 7187-7190 RP denotes out
T21270 7191-7195 IN denotes with
T21271 7196-7199 CD denotes two
T21272 7209-7218 NNS denotes reactions
T21273 7200-7208 JJ denotes separate
T21274 7219-7227 VBN denotes designed
T21275 7228-7230 TO denotes to
T21276 7231-7238 VB denotes amplify
T21277 7239-7245 CC denotes either
T21278 7257-7264 NN denotes segment
T21279 7246-7247 DT denotes a
T21280 7248-7253 CD denotes 2,748
T21281 7254-7256 NN denotes bp
T21282 7265-7269 IN denotes from
T21283 7270-7273 DT denotes the
T21284 7284-7290 NN denotes allele
T21285 7274-7278 JJ denotes wild
T21286 7279-7283 NN denotes type
T21287 7278-7279 HYPH denotes -
T21288 7291-7293 CC denotes or
T21289 7294-7295 DT denotes a
T21290 7305-7312 NN denotes segment
T21291 7296-7301 CD denotes 2,711
T21292 7302-7304 NN denotes bp
T21293 7313-7317 IN denotes from
T21294 7318-7321 DT denotes the
T21295 7329-7335 NN denotes allele
T21296 7322-7328 NN denotes mutant
T21297 7335-7336 . denotes .
T21298 7336-7494 sentence denotes Both reactions used a common sense primer located in intron 4, outside the short arm of the targeting vector, and distinct allele-specific antisense primers.
T21299 7337-7341 DT denotes Both
T21300 7342-7351 NNS denotes reactions
T21301 7352-7356 VBD denotes used
T21302 7357-7358 DT denotes a
T21303 7372-7378 NN denotes primer
T21304 7359-7365 JJ denotes common
T21305 7366-7371 NN denotes sense
T21306 7379-7386 VBN denotes located
T21307 7387-7389 IN denotes in
T21308 7390-7396 NN denotes intron
T21309 7397-7398 CD denotes 4
T21310 7398-7400 , denotes ,
T21311 7400-7407 IN denotes outside
T21312 7408-7411 DT denotes the
T21313 7418-7421 NN denotes arm
T21314 7412-7417 JJ denotes short
T21315 7422-7424 IN denotes of
T21316 7425-7428 DT denotes the
T21317 7439-7445 NN denotes vector
T21318 7429-7438 NN denotes targeting
T21319 7445-7447 , denotes ,
T21320 7447-7450 CC denotes and
T21321 7451-7459 JJ denotes distinct
T21322 7486-7493 NNS denotes primers
T21323 7460-7466 NN denotes allele
T21324 7467-7475 JJ denotes specific
T21325 7466-7467 HYPH denotes -
T21326 7476-7485 NN denotes antisense
T21327 7493-7494 . denotes .
T21328 7494-7687 sentence denotes The reaction to detect the wild-type allele used an antisense primer located in exon 7 while the amplification of the mutant sequence was done with an antisense primer in the PGK-Neo cassette.
T21329 7495-7498 DT denotes The
T21330 7499-7507 NN denotes reaction
T21331 7539-7543 VBD denotes used
T21332 7508-7510 TO denotes to
T21333 7511-7517 VB denotes detect
T21334 7518-7521 DT denotes the
T21335 7532-7538 NN denotes allele
T21336 7522-7526 JJ denotes wild
T21337 7527-7531 NN denotes type
T21338 7526-7527 HYPH denotes -
T21339 7544-7546 DT denotes an
T21340 7557-7563 NN denotes primer
T21341 7547-7556 NN denotes antisense
T21342 7564-7571 VBN denotes located
T21343 7572-7574 IN denotes in
T21344 7575-7579 NN denotes exon
T21345 7580-7581 CD denotes 7
T21346 7582-7587 IN denotes while
T21347 7633-7637 VBN denotes done
T21348 7588-7591 DT denotes the
T21349 7592-7605 NN denotes amplification
T21350 7606-7608 IN denotes of
T21351 7609-7612 DT denotes the
T21352 7620-7628 NN denotes sequence
T21353 7613-7619 NN denotes mutant
T21354 7629-7632 VBD denotes was
T21355 7638-7642 IN denotes with
T21356 7643-7645 DT denotes an
T21357 7656-7662 NN denotes primer
T21358 7646-7655 NN denotes antisense
T21359 7663-7665 IN denotes in
T21360 7666-7669 DT denotes the
T21361 7678-7686 NN denotes cassette
T21362 7670-7673 NN denotes PGK
T21363 7674-7677 NN denotes Neo
T21364 7673-7674 HYPH denotes -
T21365 7686-7687 . denotes .
T21366 7687-7837 sentence denotes The results confirm the presence of the wild-type allele in all cells, whereas the mutant allele signal was observed only in the two targeted clones.
T21367 7688-7691 DT denotes The
T21368 7692-7699 NNS denotes results
T21369 7700-7707 VBP denotes confirm
T21370 7708-7711 DT denotes the
T21371 7712-7720 NN denotes presence
T21372 7721-7723 IN denotes of
T21373 7724-7727 DT denotes the
T21374 7738-7744 NN denotes allele
T21375 7728-7732 JJ denotes wild
T21376 7733-7737 NN denotes type
T21377 7732-7733 HYPH denotes -
T21378 7745-7747 IN denotes in
T21379 7748-7751 DT denotes all
T21380 7752-7757 NNS denotes cells
T21381 7757-7759 , denotes ,
T21382 7759-7766 IN denotes whereas
T21383 7796-7804 VBN denotes observed
T21384 7767-7770 DT denotes the
T21385 7785-7791 NN denotes signal
T21386 7771-7777 NN denotes mutant
T21387 7778-7784 NN denotes allele
T21388 7792-7795 VBD denotes was
T21389 7805-7809 RB denotes only
T21390 7810-7812 IN denotes in
T21391 7813-7816 DT denotes the
T21392 7830-7836 NNS denotes clones
T21393 7817-7820 CD denotes two
T21394 7821-7829 VBN denotes targeted
T21395 7836-7837 . denotes .
T21396 7837-7878 sentence denotes (M) denotes the molecular weight marker.
T21397 7838-7839 -LRB- denotes (
T21398 7842-7849 VBZ denotes denotes
T21399 7839-7840 NN denotes M
T21400 7840-7841 -RRB- denotes )
T21401 7850-7853 DT denotes the
T21402 7871-7877 NN denotes marker
T21403 7854-7863 JJ denotes molecular
T21404 7864-7870 NN denotes weight
T21405 7877-7878 . denotes .
T22898 12521-12562 sentence denotes (M) denotes the molecular weight marker.
T22897 12520-12521 . denotes .
T22896 12508-12509 HYPH denotes -
T22895 12509-12513 NN denotes type
T22936 9708-9716 NN denotes Genotype
T22937 9717-9729 NN denotes distribution
T22938 9730-9732 IN denotes of
T22939 9733-9742 NN denotes offspring
T22940 9743-9750 VBN denotes derived
T22941 9751-9755 IN denotes from
T22942 9756-9761 NN denotes Capn2
T22943 9773-9777 NNS denotes mice
T22944 9762-9772 JJ denotes transgenic
T22945 9777-9778 . denotes .
T23680 34462-34467 NN denotes Capn2
T23679 34468-34473 NN denotes locus
T23678 34458-34461 DT denotes the
T23677 34449-34457 VB denotes genotype
T23676 34446-34448 TO denotes to
T23675 34441-34445 VBN denotes used
T23674 34433-34440 NNS denotes primers
T23673 34417-34432 NN denotes Oligonucleotide
T6986 9619-9623 VB denotes have
T6987 9632-9633 DT denotes a
T6988 9640-9646 NN denotes number
T6989 9634-9639 JJ denotes small
T6990 9670-9677 VB denotes survive
T6991 9647-9649 IN denotes of
T6992 9650-9655 NN denotes Capn2
T6993 9659-9666 NNS denotes embryos
T6994 9655-9656 SYM denotes -
T6995 9656-9657 HYPH denotes /
T6996 9657-9658 SYM denotes -
T6997 9667-9669 TO denotes to
T6998 9678-9680 IN denotes to
T6999 9681-9684 DT denotes the
T7000 9692-9697 NN denotes stage
T7001 9685-9691 NN denotes morula
T7002 9691-9692 HYPH denotes -
T7003 9697-9698 . denotes .
T7016 12624-12640 JJ denotes pre-implantation
T7015 12620-12623 CC denotes and
T7014 12618-12620 , denotes ,
T7013 12641-12648 NNS denotes embryos
T7012 12601-12618 JJ denotes post-implantation
T7011 12599-12601 , denotes ,
T7010 12590-12599 NNS denotes weanlings
T7009 12586-12589 IN denotes for
T7008 12578-12585 VBZ denotes results
T7007 12653-12658 VBN denotes shown
T7006 12567-12577 NN denotes genotyping
T7005 9699-12566 DT denotes Table 2 Genotype distribution of offspring derived from Capn2 transgenic mice. Cross (N) Age Genotype +/+ +/- -/- ND* (+/-)♀ × (+/-)♂ 199 3 weeks 23 176 0 0 (+/+)♀ × (+/-)♂ 122 3 weeks 50 72 0 0 (+/-)♀ × (+/+)♂ 103 3 weeks 28 75 0 0 (+/-)♀ × (+/-)♂ 9 E18.5 0 7 0 2 (+/-)♀ × (+/-)♂ 5 E17.5 0 5 0 0 (+/-)♀ × (+/-)♂ 9 E14.5 2 7 0 0 (+/-)♀ × (+/-)♂ 10 E11.5 3 7 0 0 (+/-)♀ × (+/-)♂ 7 E10.5 2 5 0 0 (+/-)♀ × (+/-)♂ 46 E3.5 7 39 0 0 (+/-)♀ × (+/-)♂ 48 E2.5 8 34 2 4 * ND, not determined Figure 4 Nested PCR strategy for genotyping of pre-implantation embryos. Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos. Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector. In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control. The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific. The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions. Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm. A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele. Figure 5 Genotyping of pre-implantation embryos. A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4. Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs. Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K. In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles. The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control. (A) A representative example of the genotyping of blastocyst stage embryos. Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal. (B) An example of the genotyping of 8-cell embryos is shown. Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal. (M) denotes the molecular weight marker. The
T7004 9698-12670 sentence denotes Table 2 Genotype distribution of offspring derived from Capn2 transgenic mice. Cross (N) Age Genotype +/+ +/- -/- ND* (+/-)♀ × (+/-)♂ 199 3 weeks 23 176 0 0 (+/+)♀ × (+/-)♂ 122 3 weeks 50 72 0 0 (+/-)♀ × (+/+)♂ 103 3 weeks 28 75 0 0 (+/-)♀ × (+/-)♂ 9 E18.5 0 7 0 2 (+/-)♀ × (+/-)♂ 5 E17.5 0 5 0 0 (+/-)♀ × (+/-)♂ 9 E14.5 2 7 0 0 (+/-)♀ × (+/-)♂ 10 E11.5 3 7 0 0 (+/-)♀ × (+/-)♂ 7 E10.5 2 5 0 0 (+/-)♀ × (+/-)♂ 46 E3.5 7 39 0 0 (+/-)♀ × (+/-)♂ 48 E2.5 8 34 2 4 * ND, not determined Figure 4 Nested PCR strategy for genotyping of pre-implantation embryos. Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos. Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector. In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control. The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific. The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions. Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm. A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele. Figure 5 Genotyping of pre-implantation embryos. A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4. Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs. Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K. In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles. The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control. (A) A representative example of the genotyping of blastocyst stage embryos. Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal. (B) An example of the genotyping of 8-cell embryos is shown. Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal. (M) denotes the molecular weight marker. The genotyping results for weanlings, post-implantation, and pre-implantation embryos are shown in Table 2.
T19803 34338-34341 CD denotes one
T19802 34334-34337 CC denotes and
T19801 34332-34334 , denotes ,
T19800 34330-34332 NN denotes °C
T19799 34328-34330 CD denotes 56
T19798 34325-34327 IN denotes at
T19797 34315-34324 VBG denotes annealing
T19796 34308-34314 NN denotes minute
T19795 34304-34307 CD denotes one
T19794 34302-34304 , denotes ,
R282 T3323 T3320 nummod 80,k
R287 T3324 T3320 punct ),k
R288 T3325 T3306 punct ", ",encoded
R289 T3326 T3306 advmod respectively,encoded
R290 T3244 T3245 det The,proteases
R291 T3327 T3301 punct ", ",subunits
R292 T3245 T3254 nsubj proteases,are
R293 T3246 T3245 nummod two,proteases
R294 T3328 T3329 dep that,associate
R295 T3247 T3245 amod ubiquitous,proteases
R296 T3248 T3249 npadvmod Ca2,dependent
R297 T3329 T3301 relcl associate,subunits
R298 T3249 T3245 amod dependent,proteases
R299 T3250 T3248 punct +,Ca2
R300 T3251 T3249 punct -,dependent
R301 T3330 T3329 prep with,associate
R302 T3252 T3245 punct ", ",proteases
R303 T3253 T3245 compound cysteine,proteases
R304 T3255 T3245 acl known,proteases
R305 T3331 T3332 det a,subunit
R306 T3256 T3255 prep as,known
R307 T3257 T3258 compound μ,calpain
R308 T3258 T3256 pobj calpain,as
R309 T3332 T3330 pobj subunit,with
R310 T3259 T3258 punct -,calpain
R311 T3260 T3258 punct (,calpain
R312 T3261 T3258 appos calpain,calpain
R313 T3333 T3332 amod common,subunit
R314 T3262 T3261 punct -,calpain
R315 T3263 T3261 nummod 1,calpain
R316 T3264 T3258 punct ),calpain
R317 T3334 T3335 nummod 28,kDa
R318 T3265 T3258 cc and,calpain
R319 T3335 T3332 nmod kDa,subunit
R320 T3266 T3267 compound m,calpain
R321 T3267 T3258 conj calpain,calpain
R322 T3268 T3267 punct -,calpain
R323 T3269 T3267 punct (,calpain
R324 T3270 T3267 appos capain,calpain
R325 T3271 T3270 punct -,capain
R326 T3272 T3270 nummod 2,capain
R327 T3273 T3254 punct ),are
R328 T3274 T3254 punct ", ",are
R329 T3275 T3276 det the,members
R330 T3276 T3254 attr members,are
R331 T3277 T3276 compound founding,members
R332 T3278 T3276 prep of,members
R333 T3336 T3332 amod regulatory,subunit
R334 T3279 T3280 det a,family
R335 T3280 T3278 pobj family,of
R336 T3281 T3280 compound gene,family
R337 T3282 T3280 acl comprising,family
R338 T3337 T3332 acl encoded,subunit
R339 T3283 T3284 nummod 13,genes
R340 T3284 T3282 dobj genes,comprising
R341 T3285 T3282 prep in,comprising
R342 T3338 T3337 agent by,encoded
R343 T3286 T3285 pobj mammals,in
R344 T3287 T3288 punct [,1
R345 T3288 T3254 parataxis 1,are
R346 T3339 T3340 det the,gene
R347 T3289 T3290 punct -,3
R348 T3290 T3288 prep 3,1
R349 T3291 T3288 punct ],1
R350 T3340 T3338 pobj gene,by
R351 T3292 T3254 punct .,are
R352 T3341 T3340 compound Capn4,gene
R353 T3294 T3295 nsubj Both,are
R354 T3296 T3297 amod heterodimeric,enzymes
R355 T3342 T3295 punct .,are
R356 T3297 T3295 attr enzymes,are
R357 T3298 T3297 acl consisting,enzymes
R358 T3299 T3298 prep of,consisting
R359 T3344 T3345 det The,subunits
R360 T3300 T3301 amod distinct,subunits
R361 T3301 T3299 pobj subunits,of
R362 T3302 T3303 nummod 80,kDa
R363 T3303 T3301 nmod kDa,subunits
R364 T3345 T3355 nsubj subunits,share
R365 T3304 T3301 amod catalytic,subunits
R366 T3305 T3301 punct ", ",subunits
R367 T3346 T3347 nmod μ,k
R368 T3347 T3345 nmod k,subunits
R369 T3306 T3301 acl encoded,subunits
R370 T3307 T3306 agent by,encoded
R371 T3348 T3346 punct -,μ
R372 T3308 T3309 det the,genes
R373 T3309 T3307 pobj genes,by
R374 T3310 T3309 nmod Capn1,genes
R375 T3311 T3312 punct (,k
R376 T3349 T3346 nummod 80,μ
R377 T3312 T3310 parataxis k,Capn1
R378 T3313 T3312 nmod μ,k
R379 T3314 T3312 punct -,k
R380 T3315 T3312 nummod 80,k
R381 T3350 T3347 cc and,k
R382 T3316 T3312 punct ),k
R383 T3317 T3310 cc and,Capn1
R384 T3318 T3310 conj Capn2,Capn1
R385 T3351 T3352 nmod m,k
R386 T3319 T3320 punct (,k
R387 T3320 T3318 parataxis k,Capn2
R388 T3321 T3320 nmod m,k
R389 T3352 T3347 conj k,k
R390 T3322 T3320 punct -,k
R391 T3353 T3351 punct -,m
R392 T3354 T3351 nummod 80,m
R393 T3356 T3357 nummod 62,%
R394 T3429 T3419 punct ;,levels
R395 T3357 T3358 compound %,identity
R396 T3430 T3431 quantmod 10,50
R397 T3431 T3433 nummod 50,μM
R398 T3358 T3355 dobj identity,share
R399 T3432 T3431 punct –,50
R400 T3433 T3434 compound μM,Ca2
R401 T3434 T3419 appos Ca2,levels
R402 T3359 T3360 compound amino,acid
R403 T3435 T3434 punct +,Ca2
R404 T3436 T3434 prep for,Ca2
R405 T3437 T3438 compound μ,calpain
R406 T3360 T3358 compound acid,identity
R407 T3438 T3436 pobj calpain,for
R408 T3439 T3438 punct -,calpain
R409 T3440 T3434 cc and,Ca2
R410 T3361 T3358 compound sequence,identity
R411 T3441 T3442 quantmod 0.3,0.35
R412 T3442 T3444 nummod 0.35,mM
R413 T3443 T3442 punct –,0.35
R414 T3362 T3355 punct ", ",share
R415 T3444 T3445 compound mM,Ca2
R416 T3445 T3434 conj Ca2,Ca2
R417 T3363 T3355 cc and,share
R418 T3446 T3445 punct +,Ca2
R419 T3447 T3445 prep for,Ca2
R420 T3448 T3449 compound m,calpain
R421 T3449 T3447 pobj calpain,for
R422 T3364 T3355 conj are,share
R423 T3450 T3449 punct -,calpain
R424 T3451 T3416 punct .,derive
R425 T3453 T3454 nsubjpass It,assumed
R426 T3365 T3366 advmod very,similar
R427 T3455 T3454 auxpass is,assumed
R428 T3456 T3454 advmod generally,assumed
R429 T3457 T3458 mark that,maintain
R430 T3366 T3364 acomp similar,are
R431 T3458 T3454 ccomp maintain,assumed
R432 T3459 T3460 nmod μ,calpain
R433 T3367 T3364 prep in,are
R434 T3460 T3458 nsubj calpain,maintain
R435 T3461 T3459 punct -,μ
R436 T3462 T3459 cc and,μ
R437 T3463 T3459 conj m,μ
R438 T3368 T3367 pobj terms,in
R439 T3464 T3460 punct -,calpain
R440 T3465 T3466 poss their,sensitivities
R441 T3466 T3458 dobj sensitivities,maintain
R442 T3369 T3368 prep of,terms
R443 T3467 T3466 amod differential,sensitivities
R444 T3468 T3466 prep to,sensitivities
R445 T3370 T3369 pobj structure,of
R446 T3469 T3468 pobj calcium,to
R447 T3470 T3471 advmod in,vivo
R448 T3471 T3458 advmod vivo,maintain
R449 T3371 T3370 punct ", ",structure
R450 T3472 T3458 punct ", ",maintain
R451 T3473 T3474 mark although,demonstrated
R452 T3372 T3373 compound protein,chemistry
R453 T3373 T3370 conj chemistry,structure
R454 T3474 T3458 advcl demonstrated,maintain
R455 T3475 T3474 nsubjpass this,demonstrated
R456 T3476 T3474 aux has,demonstrated
R457 T3374 T3373 punct ", ",chemistry
R458 T3477 T3474 neg not,demonstrated
R459 T3478 T3474 advmod yet,demonstrated
R460 T3375 T3373 cc and,chemistry
R461 T3479 T3474 auxpass been,demonstrated
R462 T3480 T3474 advmod strictly,demonstrated
R463 T3481 T3454 punct .,assumed
R464 T3376 T3377 advmod in,vitro
R465 T3483 T3484 advmod Furthermore,assumed
R466 T3377 T3378 amod vitro,specificity
R467 T3485 T3484 punct ", ",assumed
R468 T3486 T3487 mark since,is
R469 T3378 T3373 conj specificity,chemistry
R470 T3487 T3484 advcl is,assumed
R471 T3488 T3489 det the,concentration
R472 T3379 T3378 compound substrate,specificity
R473 T3489 T3487 nsubj concentration,is
R474 T3490 T3491 amod cytoplasmic,free
R475 T3491 T3489 amod free,concentration
R476 T3380 T3355 punct .,share
R477 T3492 T3489 nmod Ca2,concentration
R478 T3493 T3492 punct +,Ca2
R479 T3494 T3487 advmod typically,is
R480 T3495 T3496 amod less,1
R481 T3496 T3498 nummod 1,μM
R482 T3497 T3496 quantmod than,1
R483 T3382 T3383 prep Despite,suggest
R484 T3498 T3487 attr μM,is
R485 T3499 T3484 punct ", ",assumed
R486 T3500 T3484 nsubjpass it,assumed
R487 T3501 T3484 auxpass is,assumed
R488 T3384 T3385 det these,similarities
R489 T3502 T3484 advmod also,assumed
R490 T3503 T3504 mark that,contribute
R491 T3504 T3484 ccomp contribute,assumed
R492 T3505 T3506 amod other,factors
R493 T3506 T3504 nsubj factors,contribute
R494 T3385 T3382 pobj similarities,Despite
R495 T3507 T3508 advmod in,vivo
R496 T3508 T3506 amod vivo,factors
R497 T3509 T3504 aux must,contribute
R498 T3510 T3504 prep to,contribute
R499 T3386 T3383 punct ", ",suggest
R500 T3511 T3510 pobj regulation,to
R501 T3512 T3511 prep of,regulation
R502 T3387 T3388 det the,patterns
R503 T3513 T3514 det these,enzymes
R504 T3514 T3512 pobj enzymes,of
R505 T3515 T3516 punct [,3
R506 T3388 T3383 nsubj patterns,suggest
R507 T3516 T3504 parataxis 3,contribute
R508 T3517 T3516 punct ],3
R509 T3518 T3484 punct .,assumed
R510 T3520 T3521 prep Without,presents
R511 T3389 T3388 amod differential,patterns
R512 T3522 T3523 nmod apriori,knowledge
R513 T3523 T3520 pobj knowledge,Without
R514 T3390 T3388 compound expression,patterns
R515 T3524 T3523 prep of,knowledge
R516 T3525 T3526 det the,factors
R517 T3526 T3524 pobj factors,of
R518 T3391 T3388 prep of,patterns
R519 T3527 T3526 acl regulating,factors
R520 T3528 T3529 compound calpain,activity
R521 T3529 T3527 dobj activity,regulating
R522 T3392 T3393 nmod μ,calpain
R523 T3530 T3526 cc or,factors
R524 T3531 T3532 poss their,substrates
R525 T3532 T3526 conj substrates,factors
R526 T3393 T3391 pobj calpain,of
R527 T3533 T3532 amod relevant,substrates
R528 T3534 T3521 punct ", ",presents
R529 T3394 T3392 punct -,μ
R530 T3535 T3521 nsubj elucidation,presents
R531 T3395 T3392 cc and,μ
R532 T3536 T3535 prep of,elucidation
R533 T3537 T3538 amod biological,functions
R534 T3538 T3536 pobj functions,of
R535 T3539 T3535 prep for,elucidation
R536 T3396 T3392 conj m,μ
R537 T3540 T3539 pobj calpains,for
R538 T3541 T3542 det a,challenge
R539 T3542 T3521 dobj challenge,presents
R540 T3397 T3393 punct -,calpain
R541 T3543 T3521 punct .,presents
R542 T3398 T3388 prep in,patterns
R543 T3545 T3546 nsubj Research,linked
R544 T3547 T3545 prep on,Research
R545 T3399 T3400 amod mammalian,tissues
R546 T3548 T3547 pobj calpains,on
R547 T3549 T3546 aux has,linked
R548 T3550 T3546 dobj them,linked
R549 T3400 T3398 pobj tissues,in
R550 T3551 T3546 prep with,linked
R551 T3552 T3553 det a,variety
R552 T3401 T3402 nsubj they,have
R553 T3553 T3551 pobj variety,with
R554 T3554 T3553 amod wide,variety
R555 T3555 T3553 prep of,variety
R556 T3402 T3383 advcl have,suggest
R557 T3556 T3555 pobj functions,of
R558 T3557 T3553 prep including,variety
R559 T3558 T3559 compound muscle,growth
R560 T3403 T3404 det some,functions
R561 T3559 T3557 pobj growth,including
R562 T3404 T3402 dobj functions,have
R563 T3560 T3559 punct ", ",growth
R564 T3405 T3406 npadvmod isoform,specific
R565 T3561 T3559 conj development,growth
R566 T3562 T3561 punct ", ",development
R567 T3563 T3561 conj degeneration,development
R568 T3406 T3404 amod specific,functions
R569 T3564 T3565 punct (,3
R570 T3407 T3406 cc and,specific
R571 T3565 T3563 parataxis 3,degeneration
R572 T3408 T3406 conj distinct,specific
R573 T3566 T3565 punct ),3
R574 T3567 T3563 punct ", ",degeneration
R575 T3409 T3383 punct .,suggest
R578 T3411 T3412 det The,designations
R579 T3570 T3569 cc and,growth
R580 T3571 T3569 conj neurodegeneration,growth
R581 T3572 T3573 punct [,4
R582 T3573 T3571 parataxis 4,neurodegeneration
R583 T3574 T3573 punct ],4
R584 T3575 T3569 punct ", ",growth
R585 T3576 T3577 compound cell,cycle
R586 T3412 T3416 nsubj designations,derive
R587 T3577 T3578 compound cycle,progression
R588 T3578 T3569 conj progression,growth
R589 T3413 T3412 nmod μ,designations
R590 T3579 T3580 punct [,6
R591 T3580 T3578 parataxis 6,progression
R592 T3581 T3580 nummod 5,6
R593 T3414 T3413 cc and,μ
R594 T3582 T3580 punct ",",6
R595 T3583 T3580 punct ],6
R596 T3584 T3578 punct ", ",progression
R597 T3415 T3413 conj m,μ
R598 T3585 T3586 compound signal,cascades
R599 T3586 T3578 conj cascades,progression
R600 T3587 T3586 acl triggered,cascades
R601 T3417 T3416 prep from,derive
R602 T3588 T3587 agent by,triggered
R603 T3589 T3588 pobj integrins,by
R604 T3590 T3589 cc and,integrins
R605 T3591 T3592 compound growth,factors
R606 T3418 T3419 det the,levels
R607 T3592 T3589 conj factors,integrins
R608 T3593 T3594 punct [,7
R609 T3594 T3587 parataxis 7,triggered
R610 T3419 T3417 pobj levels,from
R611 T3595 T3594 punct ],7
R612 T3596 T3586 punct ", ",cascades
R613 T3597 T3598 compound membrane,protrusion
R614 T3598 T3586 conj protrusion,cascades
R615 T3420 T3419 prep of,levels
R616 T3599 T3600 punct [,8
R617 T3600 T3598 parataxis 8,protrusion
R618 T3421 T3420 pobj Ca2,of
R619 T3601 T3600 punct ],8
R620 T3602 T3598 punct ", ",protrusion
R621 T3603 T3598 conj remodeling,protrusion
R622 T3422 T3421 punct +,Ca2
R623 T3604 T3603 prep of,remodeling
R624 T3605 T3606 det the,cytoskeleton
R625 T3606 T3604 pobj cytoskeleton,of
R626 T3423 T3419 acl required,levels
R627 T3607 T3603 cc and,remodeling
R628 T3608 T3609 compound cell,migration
R629 T3609 T3603 conj migration,remodeling
R630 T3424 T3425 advmod in,vitro
R631 T3610 T3611 punct [,9
R632 T3611 T3609 parataxis 9,migration
R633 T3612 T3613 punct -,15
R634 T3613 T3611 appos 15,9
R635 T3614 T3611 punct ],9
R636 T3615 T3603 punct ", ",remodeling
R637 T3425 T3423 advmod vitro,required
R638 T3616 T3603 cc and,remodeling
R639 T3617 T3603 conj regulation,remodeling
R640 T3426 T3423 prep for,required
R641 T3618 T3617 prep of,regulation
R642 T3619 T3620 compound cell,death
R643 T3620 T3618 pobj death,of
R644 T3621 T3617 prep via,regulation
R645 T3427 T3428 amod optimal,activation
R646 T3622 T3623 preconj both,necrosis
R647 T3623 T3621 pobj necrosis,via
R648 T3624 T3623 cc and,necrosis
R649 T3428 T3426 pobj activation,for
R650 T3625 T3623 conj apoptosis,necrosis
R651 T3626 T3627 punct [,16
R652 T3627 T3617 parataxis 16,regulation
R653 T3640 T3634 dobj interplay,suggests
R654 T3628 T3629 punct -,22
R655 T3629 T3627 appos 22,16
R656 T3630 T3627 punct ],16
R657 T3631 T3546 punct .,linked
R658 T3641 T3640 amod complex,interplay
R659 T3633 T3634 prep To,suggests
R660 T3642 T3640 prep between,interplay
R661 T3635 T3633 pobj date,To
R662 T3636 T3634 punct ", ",suggests
R663 T3643 T3642 pobj caspases,between
R664 T3637 T3638 det the,literature
R665 T3638 T3634 nsubj literature,suggests
R666 T3639 T3640 det a,interplay
R667 T3644 T3643 cc and,caspases
R668 T3645 T3643 conj calpains,caspases
R669 T3646 T3647 punct [,24
R670 T3647 T3640 parataxis 24,interplay
R671 T3746 T3745 punct -,calpain
R672 T3747 T3742 prep in,role
R673 T3648 T3647 nummod 23,24
R674 T3748 T3749 amod epidermal,factor
R675 T3749 T3751 npadvmod factor,induced
R676 T3750 T3749 compound growth,factor
R677 T3649 T3647 punct ",",24
R678 T3751 T3756 amod induced,motility
R679 T3752 T3749 punct (,factor
R680 T3650 T3647 punct ],24
R681 T3753 T3749 appos EGF,factor
R682 T3754 T3751 punct ),induced
R683 T3755 T3751 punct -,induced
R684 T3651 T3640 cc and,interplay
R685 T3756 T3747 pobj motility,in
R686 T3757 T3756 compound cell,motility
R687 T3758 T3759 punct [,29
R688 T3759 T3742 parataxis 29,role
R689 T3760 T3759 nummod 28,29
R690 T3761 T3759 punct ",",29
R691 T3652 T3640 conj impact,interplay
R692 T3762 T3759 punct ],29
R693 T3763 T3742 cc and,role
R694 T3764 T3765 det a,role
R695 T3653 T3652 prep of,impact
R696 T3765 T3742 conj role,role
R697 T3766 T3765 prep for,role
R698 T3654 T3653 pobj calpain,of
R699 T3767 T3768 compound μ,calpain
R700 T3768 T3766 pobj calpain,for
R701 T3769 T3768 punct -,calpain
R702 T3655 T3652 prep on,impact
R703 T3770 T3765 prep in,role
R704 T3771 T3772 npadvmod interferon,inducible
R705 T3772 T3774 amod inducible,migration
R706 T3656 T3657 compound cell,death
R707 T3773 T3772 punct -,inducible
R708 T3774 T3770 pobj migration,in
R709 T3775 T3776 npadvmod protein,induced
R710 T3657 T3658 compound death,components
R711 T3776 T3774 amod induced,migration
R712 T3777 T3775 nummod 9,protein
R713 T3778 T3776 punct -,induced
R714 T3658 T3655 pobj components,on
R715 T3779 T3774 prep of,migration
R716 T3780 T3779 pobj keratinocytes,of
R717 T3781 T3782 punct [,28
R718 T3659 T3658 compound pathway,components
R719 T3782 T3765 parataxis 28,role
R720 T3783 T3782 punct ],28
R721 T3784 T3733 punct .,suggested
R722 T3660 T3661 punct [,25
R723 T3786 T3787 det A,inhibitor
R724 T3661 T3652 parataxis 25,impact
R725 T3787 T3791 nsubjpass inhibitor,used
R726 T3788 T3789 npadvmod cell,permeable
R727 T3789 T3787 amod permeable,inhibitor
R728 T3790 T3787 compound calpain,inhibitor
R729 T3662 T3661 punct ],25
R730 T3792 T3787 punct (,inhibitor
R731 T3663 T3634 punct .,suggests
R732 T3793 T3794 dep which,inhibits
R733 T3794 T3787 relcl inhibits,inhibitor
R734 T3795 T3794 advmod likely,inhibits
R735 T3665 T3666 det The,lack
R736 T3796 T3797 amod other,proteases
R737 T3797 T3794 dobj proteases,inhibits
R738 T3798 T3797 compound thiol,proteases
R739 T3799 T3797 punct -,proteases
R740 T3800 T3801 advmod as,well
R741 T3801 T3794 advmod well,inhibits
R742 T3802 T3791 punct ),used
R743 T3666 T3667 nsubj lack,contributes
R744 T3803 T3791 aux has,used
R745 T3804 T3791 auxpass been,used
R746 T3805 T3806 aux to,select
R747 T3668 T3666 prep of,lack
R748 T3806 T3791 advcl select,used
R749 T3807 T3806 dobj cells,select
R750 T3808 T3807 acl lacking,cells
R751 T3809 T3810 compound μ,calpain
R752 T3669 T3670 advmod highly,specific
R753 T3670 T3671 amod specific,inhibitors
R754 T3810 T3808 dobj calpain,lacking
R755 T3671 T3668 pobj inhibitors,of
R756 T3811 T3810 punct -,calpain
R757 T3812 T3813 dep which,display
R758 T3813 T3807 relcl display,cells
R759 T3672 T3673 npadvmod cell,permeable
R760 T3814 T3815 amod reduced,rates
R761 T3815 T3813 dobj rates,display
R762 T3816 T3815 compound proliferation,rates
R763 T3673 T3671 amod permeable,inhibitors
R764 T3817 T3818 punct [,30
R765 T3818 T3813 parataxis 30,display
R766 T3819 T3818 punct ],30
R767 T3674 T3673 punct -,permeable
R768 T3820 T3791 punct .,used
R769 T3675 T3671 prep of,inhibitors
R770 T3822 T3823 advmod Interestingly,persisted
R771 T3676 T3675 pobj calpains,of
R772 T3824 T3823 punct ", ",persisted
R773 T3825 T3826 compound m,calpain
R774 T3826 T3828 compound calpain,expression
R775 T3677 T3667 prep to,contributes
R776 T3827 T3826 punct -,calpain
R777 T3828 T3823 nsubj expression,persisted
R778 T3829 T3823 prep in,persisted
R779 T3678 T3679 det the,challenge
R780 T3830 T3831 det these,cells
R781 T3831 T3829 pobj cells,in
R782 T3832 T3823 punct ", ",persisted
R783 T3679 T3677 pobj challenge,to
R784 T3833 T3823 advcl suggesting,persisted
R785 T3834 T3835 det a,requirement
R786 T3835 T3833 dobj requirement,suggesting
R787 T3680 T3679 prep of,challenge
R788 T3836 T3835 amod possible,requirement
R789 T3837 T3835 prep of,requirement
R790 T3681 T3680 pcomp investigating,of
R791 T3838 T3839 compound m,calpain
R792 T3839 T3837 pobj calpain,of
R793 T3840 T3839 punct -,calpain
R794 T3841 T3835 prep for,requirement
R795 T3842 T3843 compound cell,survival
R796 T3843 T3841 pobj survival,for
R797 T3682 T3681 cc and,investigating
R798 T3844 T3845 punct [,30
R799 T3845 T3823 parataxis 30,persisted
R800 T3846 T3845 punct ],30
R801 T3683 T3681 conj defining,investigating
R802 T3847 T3823 punct .,persisted
R803 T3684 T3685 compound calpain,functions
R804 T3849 T3850 amod Targeted,deletion
R805 T3685 T3683 dobj functions,defining
R806 T3686 T3683 prep in,defining
R807 T3850 T3852 nsubj deletion,provides
R808 T3687 T3688 det these,processes
R809 T3851 T3850 compound gene,deletion
R810 T3853 T3850 prep in,deletion
R811 T3688 T3686 pobj processes,in
R812 T3854 T3853 pobj mice,in
R813 T3855 T3856 det a,approach
R814 T3689 T3667 punct .,contributes
R815 T3856 T3852 dobj approach,provides
R816 T3691 T3692 mark Although,provides
R817 T3857 T3856 amod powerful,approach
R818 T3858 T3856 prep to,approach
R819 T3859 T3858 pcomp determining,to
R820 T3860 T3861 det the,roles
R821 T3692 T3708 advcl provides,distinguish
R822 T3861 T3859 dobj roles,determining
R823 T3862 T3861 amod physiological,roles
R824 T3863 T3861 prep of,roles
R825 T3693 T3692 nsubj over-expression,provides
R826 T3864 T3865 nmod μ,calpain
R827 T3865 T3863 pobj calpain,of
R828 T3694 T3693 prep of,over-expression
R829 T3866 T3864 punct -,μ
R830 T3867 T3864 cc and,μ
R831 T3868 T3864 conj m,μ
R832 T3695 T3694 pobj calpastatin,of
R833 T3869 T3865 punct -,calpain
R834 T3870 T3856 cc and,approach
R835 T3871 T3872 det the,opportunity
R836 T3696 T3695 punct ", ",calpastatin
R837 T3872 T3856 conj opportunity,approach
R838 T3873 T3874 aux to,approach
R839 T3697 T3698 det the,inhibitor
R840 T3874 T3872 acl approach,opportunity
R841 T3875 T3876 poss their,functions
R842 T3876 T3874 dobj functions,approach
R843 T3877 T3878 npadvmod isoform,specific
R844 T3878 T3876 amod specific,functions
R845 T3879 T3852 punct .,provides
R846 T3698 T3695 appos inhibitor,calpastatin
R847 T3881 T3882 amod Initial,studies
R848 T3882 T3883 nsubj studies,targeted
R849 T3699 T3698 amod endogenous,inhibitor
R850 T3884 T3883 dobj Capn4,targeted
R851 T3885 T3883 prep based,targeted
R852 T3700 T3698 compound protein,inhibitor
R853 T3886 T3885 prep on,based
R854 T3887 T3888 det the,prediction
R855 T3888 T3886 pobj prediction,on
R856 T3889 T3890 mark that,abolish
R857 T3701 T3698 prep of,inhibitor
R858 T3890 T3888 acl abolish,prediction
R859 T3702 T3703 nmod μ,calpain
R860 T3891 T3890 nsubj loss,abolish
R861 T3892 T3891 prep of,loss
R862 T3893 T3894 det this,subunit
R863 T3894 T3892 pobj subunit,of
R864 T3703 T3701 pobj calpain,of
R865 T3895 T3894 compound calpain,subunit
R866 T3896 T3890 aux would,abolish
R867 T3704 T3702 punct -,μ
R868 T3897 T3890 dobj activity,abolish
R869 T3705 T3702 cc and,μ
R870 T3898 T3897 prep of,activity
R871 T3899 T3900 preconj both,μ
R872 T3706 T3702 conj m,μ
R873 T3900 T3901 nmod μ,calpain
R874 T3901 T3898 pobj calpain,of
R875 T3707 T3703 punct -,calpain
R876 T3902 T3900 punct -,μ
R877 T3903 T3900 cc and,μ
R878 T3904 T3900 conj m,μ
R879 T3709 T3710 det an,approach
R880 T3905 T3901 punct -,calpain
R881 T3906 T3883 punct .,targeted
R882 T3908 T3909 nmod Capn4,embryos
R883 T3710 T3692 dobj approach,provides
R884 T3909 T3914 nsubj embryos,died
R885 T3910 T3908 punct -,Capn4
R886 T3911 T3908 punct /,Capn4
R887 T3711 T3710 amod important,approach
R888 T3912 T3908 punct -,Capn4
R889 T3913 T3909 amod murine,embryos
R890 T3712 T3710 prep for,approach
R891 T3915 T3914 prep between,died
R892 T3916 T3917 nmod days,10
R893 T3713 T3714 det these,efforts
R894 T3917 T3915 pobj 10,between
R895 T3918 T3917 cc and,10
R896 T3919 T3917 conj 11,10
R897 T3920 T3917 prep of,10
R898 T3921 T3920 pobj gestation,of
R899 T3922 T3914 punct ", ",died
R900 T3714 T3712 pobj efforts,for
R901 T3923 T3914 cc and,died
R902 T3924 T3925 expl there,was
R903 T3925 T3914 conj was,died
R904 T3715 T3708 punct ", ",distinguish
R905 T3926 T3927 det no,activity
R906 T3927 T3925 attr activity,was
R907 T3928 T3927 amod detectable,activity
R908 T3716 T3708 nsubj it,distinguish
R909 T3929 T3930 nmod μ,calpain
R910 T3930 T3927 compound calpain,activity
R911 T3931 T3929 punct -,μ
R912 T3717 T3708 aux will,distinguish
R913 T3932 T3929 cc or,μ
R914 T3933 T3929 conj m,μ
R915 T3718 T3708 neg not,distinguish
R916 T3934 T3930 punct -,calpain
R917 T3935 T3925 prep in,was
R918 T3936 T3935 pobj these,in
R919 T3937 T3936 cc or,these
R920 T3719 T3720 npadvmod isoform,specific
R921 T3938 T3939 amod younger,embryos
R922 T3939 T3936 conj embryos,these
R923 T3720 T3721 amod specific,functions
R924 T3940 T3941 punct [,31
R925 T3941 T3925 parataxis 31,was
R926 T3942 T3941 punct ],31
R927 T3721 T3708 dobj functions,distinguish
R928 T3943 T3925 punct .,was
R929 T3945 T3946 nsubjpass Capn4,cultured
R930 T3722 T3723 punct [,27
R931 T3947 T3945 punct -,Capn4
R932 T3948 T3945 punct /,Capn4
R933 T3723 T3708 parataxis 27,distinguish
R934 T3949 T3945 punct -,Capn4
R935 T3950 T3951 amod murine,fibroblasts
R936 T3724 T3723 nummod 24,27
R937 T3725 T3723 punct ",",27
R938 T3951 T3945 appos fibroblasts,Capn4
R939 T3726 T3723 nummod 26,27
R940 T3952 T3951 amod embryonic,fibroblasts
R941 T3727 T3723 punct ",",27
R942 T3953 T3951 punct (,fibroblasts
R943 T3954 T3951 appos MEFs,fibroblasts
R944 T3728 T3723 punct ],27
R945 T3729 T3708 punct .,distinguish
R946 T3955 T3946 punct ),cultured
R947 T3731 T3732 det Some,work
R948 T3956 T3946 aux could,cultured
R949 T3957 T3946 auxpass be,cultured
R950 T3958 T3946 prep from,cultured
R951 T3959 T3960 det these,embryos
R952 T3960 T3958 pobj embryos,from
R953 T3961 T3946 punct ", ",cultured
R954 T3962 T3963 mark although,lacked
R955 T3732 T3733 nsubj work,suggested
R956 T3963 T3946 advcl lacked,cultured
R957 T3964 T3963 nsubj they,lacked
R958 T3965 T3963 advmod also,lacked
R959 T3734 T3733 aux has,suggested
R960 T3966 T3967 compound calpain,activity
R961 T3967 T3963 dobj activity,lacked
R962 T3968 T3969 mark as,assessed
R963 T3969 T3963 advcl assessed,lacked
R964 T3735 T3736 npadvmod isoform,specific
R965 T3970 T3969 prep by,assessed
R966 T3971 T3972 compound casein,zymography
R967 T3972 T3970 pobj zymography,by
R968 T3736 T3737 amod specific,roles
R969 T3973 T3970 cc or,by
R970 T3974 T3970 conj by,by
R971 T3737 T3733 dobj roles,suggested
R972 T3975 T3976 det the,formation
R973 T3976 T3974 pobj formation,by
R974 T3738 T3737 punct ", ",roles
R975 T3977 T3976 prep of,formation
R976 T3978 T3979 amod characteristic,products
R977 T3979 T3977 pobj products,of
R978 T3739 T3740 amod such,as
R979 T3980 T3981 compound spectrin,breakdown
R980 T3981 T3979 compound breakdown,products
R981 T3740 T3737 prep as,roles
R982 T3741 T3742 det a,role
R983 T3982 T3963 punct ", ",lacked
R984 T3983 T3963 cc and,lacked
R985 T3742 T3740 pobj role,as
R986 T3984 T3985 nsubj they,displayed
R987 T3985 T3963 conj displayed,lacked
R988 T3986 T3987 compound migration,defects
R989 T3743 T3742 prep for,role
R990 T3987 T3985 dobj defects,displayed
R991 T3988 T3987 amod consistent,defects
R992 T3989 T3988 prep with,consistent
R993 T3744 T3745 compound m,calpain
R994 T3990 T3991 det a,role
R995 T3991 T3989 pobj role,with
R996 T3745 T3743 pobj calpain,for
R997 T3992 T3991 prep for,role
R998 T3993 T3992 pobj calpain,for
R999 T3994 T3991 prep in,role
R1000 T3995 T3994 pobj release,in
R1001 T3996 T3995 prep of,release
R1002 T3997 T3998 amod focal,adhesions
R1003 T4061 T4059 punct ", ",mutation
R1004 T3998 T3996 pobj adhesions,of
R1005 T3999 T4000 punct [,9
R1006 T4000 T3985 parataxis 9,displayed
R1007 T4001 T4000 punct ],9
R1008 T4002 T3946 punct .,cultured
R1009 T4062 T4063 dep which,retained
R1010 T4004 T4005 det An,knockout
R1011 T4005 T4009 nsubj knockout,resulted
R1012 T4063 T4059 relcl retained,mutation
R1013 T4006 T4007 advmod independently,derived
R1014 T4007 T4005 amod derived,knockout
R1015 T4008 T4005 compound Capn4,knockout
R1016 T4064 T4065 det some,level
R1017 T4010 T4005 punct ", ",knockout
R1018 T4011 T4005 prep involving,knockout
R1019 T4065 T4063 dobj level,retained
R1020 T4012 T4013 det a,deletion
R1021 T4013 T4011 pobj deletion,involving
R1022 T4014 T4015 advmod more,extensive
R1023 T4066 T4065 amod small,level
R1024 T4015 T4013 amod extensive,deletion
R1025 T4016 T4013 prep of,deletion
R1026 T4067 T4065 prep of,level
R1027 T4017 T4018 det the,gene
R1028 T4018 T4016 pobj gene,of
R1029 T4019 T4009 punct ", ",resulted
R1030 T4020 T4009 prep in,resulted
R1031 T4068 T4069 compound calpain,activity
R1032 T4021 T4022 det an,lethality
R1033 T4022 T4020 pobj lethality,in
R1034 T4069 T4067 pobj activity,of
R1035 T4023 T4022 amod earlier,lethality
R1036 T4024 T4022 amod embryonic,lethality
R1037 T4025 T4009 punct ", ",resulted
R1038 T4070 T4044 punct ", ",targeted
R1039 T4026 T4027 advmod apparently,at
R1040 T4027 T4009 prep at,resulted
R1041 T4028 T4029 det a,stage
R1042 T4029 T4027 pobj stage,at
R1043 T4071 T4044 advcl allowing,targeted
R1044 T4030 T4029 amod pre-implantation,stage
R1045 T4031 T4032 punct [,32
R1046 T4032 T4009 parataxis 32,resulted
R1047 T4072 T4071 prep for,allowing
R1048 T4033 T4032 punct ],32
R1049 T4034 T4009 punct .,resulted
R1050 T4073 T4074 poss their,survival
R1051 T4036 T4037 det The,times
R1052 T4037 T4039 nsubj times,suggested
R1053 T4038 T4037 amod different,times
R1054 T4040 T4037 prep of,times
R1055 T4074 T4072 pobj survival,for
R1056 T4041 T4042 amod embryonic,lethality
R1057 T4042 T4040 pobj lethality,of
R1058 T4043 T4044 mark that,targeted
R1060 T4044 T4039 ccomp targeted,suggested
R1061 T4045 T4046 det the,mice
R1062 T4046 T4044 nsubjpass mice,targeted
R1063 T4076 T4075 pobj mid-gestation,to
R1064 T4047 T4046 amod first,mice
R1065 T4048 T4046 amod reported,mice
R1066 T4077 T4044 punct ", ",targeted
R1067 T4078 T4079 mark while,represented
R1068 T4079 T4044 advcl represented,targeted
R1069 T4080 T4081 det the,mice
R1071 T4082 T4081 amod second,mice
R1072 T4083 T4081 amod reported,mice
R1073 T4084 T4081 nmod Capn4,mice
R1074 T4085 T4084 punct -,Capn4
R1075 T4086 T4084 punct /,Capn4
R1076 T4087 T4084 punct -,Capn4
R1077 T4088 T4089 punct [,32
R1078 T4049 T4046 nmod Capn4,mice
R1079 T4089 T4081 parataxis 32,mice
R1080 T4090 T4089 punct ],32
R1081 T4050 T4049 punct -,Capn4
R1082 T4091 T4092 det a,mutation
R1083 T4051 T4049 punct /,Capn4
R1084 T4052 T4049 punct -,Capn4
R1085 T4092 T4079 dobj mutation,represented
R1086 T4053 T4054 punct [,31
R1087 T4054 T4046 parataxis 31,mice
R1088 T4093 T4092 amod true,mutation
R1089 T4055 T4054 punct ],31
R1090 T4094 T4092 amod null,mutation
R1091 T4056 T4044 auxpass were,targeted
R1092 T4057 T4044 prep with,targeted
R1093 T4058 T4059 det a,mutation
R1094 T4095 T4039 punct .,suggested
R1095 T4059 T4057 pobj mutation,with
R1096 T4060 T4059 amod hypomorphic,mutation
R1097 T4097 T4098 nsubjpass Disruption,reported
R1098 T4099 T4097 prep of,Disruption
R1099 T4100 T4099 pobj Capn1,of
R1100 T4101 T4097 punct ", ",Disruption
R1101 T4102 T4097 acl encoding,Disruption
R1102 T4168 T4169 det The,viability
R1103 T4169 T4170 nsubj viability,distinguish
R1104 T4171 T4169 prep of,viability
R1105 T4172 T4173 npadvmod Capn1,deficient
R1106 T4173 T4175 amod deficient,mice
R1107 T4174 T4173 punct -,deficient
R1108 T4103 T4104 det the,subunit
R1109 T4175 T4171 pobj mice,of
R1110 T4176 T4170 aux does,distinguish
R1111 T4177 T4170 neg not,distinguish
R1112 T4104 T4102 dobj subunit,encoding
R1113 T4178 T4170 advmod however,distinguish
R1114 T4179 T4170 prep between,distinguish
R1115 T4180 T4181 nummod two,possibilities
R1116 T4105 T4106 nmod μ,calpain
R1117 T4181 T4179 pobj possibilities,between
R1118 T4182 T4181 punct : ,possibilities
R1119 T4106 T4104 nmod calpain,subunit
R1120 T4183 T4184 preconj either,required
R1121 T4184 T4181 advcl required,possibilities
R1122 T4185 T4184 mark that,required
R1123 T4107 T4106 punct -,calpain
R1124 T4186 T4187 compound m,calpain
R1125 T4187 T4184 nsubjpass calpain,required
R1126 T4188 T4187 punct -,calpain
R1127 T4108 T4104 amod catalytic,subunit
R1128 T4189 T4184 auxpass is,required
R1129 T4190 T4184 advmod specifically,required
R1130 T4109 T4098 punct ", ",reported
R1131 T4191 T4184 prep during,required
R1132 T4192 T4191 pobj embryogenesis,during
R1133 T4193 T4184 punct ", ",required
R1134 T4110 T4098 auxpass was,reported
R1135 T4194 T4184 cc or,required
R1136 T4195 T4196 mark that,is
R1137 T4196 T4184 conj is,required
R1138 T4111 T4098 advmod subsequently,reported
R1139 T4197 T4198 preconj either,form
R1140 T4198 T4196 nsubj form,is
R1141 T4199 T4198 prep of,form
R1142 T4112 T4113 aux to,result
R1143 T4200 T4199 pobj calpain,of
R1144 T4201 T4198 advmod alone,form
R1145 T4202 T4196 acomp sufficient,is
R1146 T4113 T4098 xcomp result,reported
R1147 T4203 T4196 cc and,is
R1148 T4204 T4205 aux can,compensate
R1149 T4114 T4113 prep in,result
R1150 T4205 T4196 conj compensate,is
R1151 T4206 T4205 prep for,compensate
R1152 T4207 T4208 det the,absence
R1153 T4115 T4116 amod fertile,mice
R1154 T4208 T4206 pobj absence,for
R1155 T4209 T4208 prep of,absence
R1156 T4210 T4211 det the,other
R1157 T4211 T4209 pobj other,of
R1158 T4212 T4170 punct .,distinguish
R1159 T4116 T4114 pobj mice,in
R1160 T4214 T4215 aux To,resolve
R1161 T4215 T4216 advcl resolve,knocked
R1162 T4117 T4116 punct ", ",mice
R1163 T4217 T4218 det this,question
R1164 T4218 T4215 dobj question,resolve
R1165 T4118 T4116 amod viable,mice
R1166 T4219 T4216 punct ", ",knocked
R1167 T4220 T4216 nsubj we,knocked
R1168 T4221 T4216 aux have,knocked
R1169 T4119 T4116 prep with,mice
R1170 T4222 T4216 advmod now,knocked
R1171 T4223 T4216 prt out,knocked
R1172 T4224 T4225 det the,gene
R1173 T4120 T4121 det some,defects
R1174 T4225 T4216 dobj gene,knocked
R1175 T4226 T4225 compound Capn2,gene
R1176 T4227 T4225 acl encoding,gene
R1177 T4121 T4119 pobj defects,with
R1178 T4228 T4229 det the,subunit
R1179 T4229 T4227 dobj subunit,encoding
R1180 T4230 T4231 nmod m,k
R1181 T4122 T4121 amod mild,defects
R1182 T4231 T4229 compound k,subunit
R1183 T4232 T4231 punct -,k
R1184 T4233 T4231 nummod 80,k
R1185 T4123 T4121 prep in,defects
R1186 T4234 T4227 prep in,encoding
R1187 T4124 T4125 det the,platelets
R1188 T4125 T4123 pobj platelets,in
R1189 T4126 T4127 compound μ,calpain
R1190 T4235 T4234 pobj mice,in
R1191 T4236 T4216 punct .,knocked
R1192 T4127 T4129 npadvmod calpain,rich
R1193 T4238 T4239 nsubj We,report
R1194 T4128 T4127 punct -,calpain
R1195 T4240 T4239 advmod here,report
R1196 T4241 T4242 mark that,died
R1197 T4242 T4239 ccomp died,report
R1198 T4129 T4125 amod rich,platelets
R1199 T4243 T4244 npadvmod Capn2,null
R1200 T4244 T4245 amod null,embryos
R1201 T4130 T4121 acl relating,defects
R1202 T4245 T4242 nsubj embryos,died
R1203 T4246 T4247 amod prior,to
R1204 T4131 T4130 prep to,relating
R1205 T4247 T4242 prep to,died
R1206 T4248 T4249 det the,stage
R1207 T4249 T4247 pobj stage,to
R1208 T4250 T4249 compound implantation,stage
R1209 T4251 T4242 punct ", ",died
R1210 T4252 T4242 advcl indicating,died
R1211 T4132 T4133 poss their,aggregation
R1212 T4253 T4254 mark that,is
R1213 T4254 T4252 ccomp is,indicating
R1214 T4255 T4256 compound m,calpain
R1215 T4133 T4131 pobj aggregation,to
R1216 T4256 T4254 nsubj calpain,is
R1217 T4257 T4256 punct -,calpain
R1218 T4258 T4254 acomp indispensable,is
R1219 T4134 T4133 cc and,aggregation
R1220 T4259 T4258 prep for,indispensable
R1221 T4260 T4261 amod early,embryogenesis
R1222 T4261 T4259 pobj embryogenesis,for
R1223 T4135 T4136 compound clot,retraction
R1224 T4262 T4239 punct .,report
R1225 T4264 T4265 det This,role
R1226 T4136 T4133 conj retraction,aggregation
R1227 T4265 T4266 nsubjpass role,fulfilled
R1228 T4267 T4266 aux can,fulfilled
R1229 T4137 T4138 punct [,33
R1230 T4268 T4266 neg not,fulfilled
R1231 T4269 T4266 auxpass be,fulfilled
R1232 T4270 T4266 agent by,fulfilled
R1233 T4138 T4098 parataxis 33,reported
R1234 T4271 T4272 compound μ,calpain
R1235 T4272 T4270 pobj calpain,by
R1236 T4139 T4138 punct ],33
R1237 T4140 T4098 punct .,reported
R1238 T4142 T4143 det The,fact
R1239 T4273 T4272 punct -,calpain
R1240 T4274 T4272 punct ", ",calpain
R1241 T4275 T4276 dep which,expressed
R1242 T4143 T4144 nsubj fact,indicates
R1243 T4276 T4272 relcl expressed,calpain
R1244 T4277 T4276 auxpass is,expressed
R1245 T4278 T4276 prep in,expressed
R1246 T4145 T4146 mark that,die
R1247 T4279 T4280 amod embryonic,stem
R1248 T4280 T4281 nmod stem,cells
R1249 T4281 T4278 pobj cells,in
R1250 T4282 T4280 punct (,stem
R1251 T4146 T4143 acl die,fact
R1252 T4283 T4280 appos ES,stem
R1253 T4284 T4281 punct ),cells
R1254 T4285 T4286 punct [,31
R1255 T4147 T4148 npadvmod Capn4,null
R1256 T4286 T4276 parataxis 31,expressed
R1257 T4287 T4286 punct ],31
R1258 T4288 T4276 cc and,expressed
R1259 T4148 T4149 amod null,mice
R1260 T4289 T4290 auxpass is,assumed
R1261 T4290 T4276 conj assumed,expressed
R1262 T4291 T4292 aux to,be
R1263 T4292 T4290 xcomp be,assumed
R1264 T4293 T4292 acomp present,be
R1265 T4294 T4293 prep at,present
R1266 T4149 T4146 nsubj mice,die
R1270 T4150 T4146 prep during,die
R1271 T4298 T4297 pobj gestation,of
R1272 T4299 T4266 punct .,fulfilled
R1273 T4151 T4150 pobj embryogenesis,during
R1274 T4301 T4302 nsubj This,demonstrates
R1275 T4152 T4153 mark that,is
R1276 T4303 T4302 advmod unequivocally,demonstrates
R1277 T4304 T4305 mark that,have
R1278 T4305 T4302 ccomp have,demonstrates
R1279 T4153 T4144 ccomp is,indicates
R1280 T4306 T4307 compound m,calpain
R1281 T4307 T4305 nsubj calpain,have
R1282 T4308 T4307 punct -,calpain
R1283 T4154 T4155 advmod at,one
R1284 T4309 T4307 cc and,calpain
R1285 T4310 T4311 compound μ,calpain
R1286 T4311 T4307 conj calpain,calpain
R1287 T4155 T4153 nsubj one,is
R1288 T4312 T4311 punct -,calpain
R1289 T4313 T4314 amod distinct,roles
R1290 T4314 T4305 dobj roles,have
R1291 T4156 T4155 advmod least,one
R1292 T4315 T4314 amod physiological,roles
R1293 T4316 T4305 prep during,have
R1294 T4317 T4318 amod early,embryogenesis
R1295 T4157 T4155 prep of,one
R1296 T4318 T4316 pobj embryogenesis,during
R1297 T4319 T4302 punct .,demonstrates
R1298 T4158 T4159 det the,calpains
R1299 T4159 T4157 pobj calpains,of
R1300 T4160 T4159 amod ubiquitous,calpains
R1301 T4161 T4153 acomp essential,is
R1302 T4162 T4161 prep for,essential
R1303 T4163 T4162 pobj development,for
R1304 T4164 T4163 prep to,development
R1305 T4165 T4164 pobj term,to
R1306 T4166 T4144 punct .,indicates
R1307 T4833 T4832 cc and,Isolation
R1308 T4834 T4832 conj characterization,Isolation
R1309 T4835 T4832 prep of,Isolation
R1310 T4836 T4837 npadvmod Capn2,targeted
R1311 T4837 T4838 amod targeted,clones
R1312 T4838 T4835 pobj clones,of
R1313 T4839 T4840 compound ES,cell
R1314 T4840 T4838 compound cell,clones
R1315 T4842 T4843 nummod Two,lines
R1316 T4843 T4851 nsubjpass lines,isolated
R1317 T4844 T4843 amod independent,lines
R1318 T4845 T4843 nmod Capn2,lines
R1319 T4846 T4845 punct +,Capn2
R1320 T4847 T4845 punct /,Capn2
R1321 T4848 T4845 punct -,Capn2
R1322 T4849 T4843 compound ES,lines
R1323 T4850 T4843 compound cell,lines
R1324 T4852 T4843 punct ", ",lines
R1325 T4853 T4843 acl designated,lines
R1326 T4854 T4853 oprd ES27,designated
R1327 T4855 T4854 cc and,ES27
R1328 T4856 T4854 conj ES36,ES27
R1329 T4857 T4851 punct ", ",isolated
R1330 T4858 T4851 auxpass were,isolated
R1331 T4859 T4851 prep from,isolated
R1332 T4860 T4861 det a,screen
R1333 T4861 T4859 pobj screen,from
R1334 T4862 T4861 prep of,screen
R1335 T4863 T4864 nummod 305,clones
R1336 T4864 T4862 pobj clones,of
R1337 T4865 T4866 npadvmod drug,resistant
R1338 T4866 T4864 amod resistant,clones
R1339 T4867 T4866 punct -,resistant
R1340 T4868 T4851 punct .,isolated
R1341 T4870 T4871 amod Correct,targeting
R1342 T4871 T4872 nsubjpass targeting,established
R1343 T4873 T4871 prep of,targeting
R1344 T4874 T4875 det the,locus
R1345 T4875 T4873 pobj locus,of
R1346 T4876 T4875 compound Capn2,locus
R1347 T4877 T4872 auxpass was,established
R1348 T4878 T4879 preconj both,by
R1349 T4879 T4872 agent by,established
R1350 T4880 T4881 compound Southern,blot
R1351 T4881 T4882 compound blot,hybridization
R1352 T4882 T4879 pobj hybridization,by
R1353 T4883 T4882 cc and,hybridization
R1354 T4884 T4885 compound PCR,analysis
R1355 T4885 T4882 conj analysis,hybridization
R1356 T4886 T4872 punct .,established
R1357 T4888 T4889 det A,probe
R1358 T4889 T4890 nsubj probe,hybridized
R1359 T4891 T4889 acl located,probe
R1360 T4892 T4891 advmod outside,located
R1361 T4893 T4894 det the,arm
R1362 T4894 T4892 npadvmod arm,outside
R1363 T4895 T4894 amod short,arm
R1364 T4896 T4897 punct (,upstream
R1365 T4897 T4895 parataxis upstream,short
R1366 T4898 T4897 punct ),upstream
R1367 T4899 T4894 prep of,arm
R1368 T4900 T4899 pobj homology,of
R1369 T4901 T4890 prep to,hybridized
R1370 T4902 T4903 det a,fragment
R1371 T4903 T4901 pobj fragment,to
R1372 T4904 T4905 nummod 3.5,kb
R1373 T4905 T4903 compound kb,fragment
R1374 T4906 T4905 punct -,kb
R1375 T4907 T4903 compound BamHI,fragment
R1376 T4908 T4903 prep of,fragment
R1377 T4909 T4910 det the,allele
R1378 T4910 T4908 pobj allele,of
R1379 T4911 T4912 amod wild,type
R1380 T4912 T4910 compound type,allele
R1381 T4913 T4912 punct -,type
R1382 T4914 T4903 cc and,fragment
R1383 T4915 T4916 nummod 5.3,kb
R1384 T4916 T4918 compound kb,fragment
R1385 T4917 T4916 punct -,kb
R1386 T4918 T4903 conj fragment,fragment
R1387 T4919 T4918 compound BamHI,fragment
R1388 T4920 T4918 prep of,fragment
R1389 T4921 T4922 det the,allele
R1390 T4922 T4920 pobj allele,of
R1391 T4923 T4922 compound mutant,allele
R1392 T4924 T4925 mark as,predicted
R1393 T4925 T4890 advcl predicted,hybridized
R1394 T4926 T4925 prep from,predicted
R1395 T4927 T4928 amod genomic,maps
R1396 T4928 T4926 pobj maps,from
R1397 T4929 T4930 punct (,2A
R1398 T4930 T4925 parataxis 2A,predicted
R1399 T4931 T4930 compound Figure,2A
R1400 T4932 T4930 punct ),2A
R1401 T4933 T4890 punct .,hybridized
R1402 T4935 T4936 det The,probe
R1403 T4936 T4938 nsubj probe,detected
R1404 T4937 T4936 amod same,probe
R1405 T4939 T4938 advmod also,detected
R1406 T4940 T4941 det the,fragments
R1407 T4941 T4938 dobj fragments,detected
R1408 T4942 T4941 amod expected,fragments
R1409 T4943 T4944 nummod 7.2,kb
R1410 T4944 T4941 nmod kb,fragments
R1411 T4945 T4944 punct -,kb
R1412 T4946 T4947 amod wild,type
R1413 T4947 T4944 appos type,kb
R1414 T4948 T4947 punct -,type
R1415 T4949 T4944 cc and,kb
R1416 T4950 T4951 nummod 6.4,kb
R1417 T4951 T4953 compound kb,mutant
R1418 T4952 T4951 punct -,kb
R1419 T4953 T4944 conj mutant,kb
R1420 T4954 T4941 compound BglII,fragments
R1421 T4955 T4941 punct ", ",fragments
R1422 T4956 T4957 nummod 4.9,kb
R1423 T4957 T4959 nmod kb,fragments
R1424 T4958 T4957 punct -,kb
R1425 T4959 T4941 conj fragments,fragments
R1426 T4960 T4961 amod wild,type
R1427 T4961 T4957 appos type,kb
R1428 T4962 T4961 punct -,type
R1429 T4963 T4957 cc and,kb
R1430 T4964 T4965 nummod 5.7,kb
R1431 T4965 T4967 compound kb,mutant
R1432 T4966 T4965 punct -,kb
R1433 T4967 T4957 conj mutant,kb
R1434 T4968 T4959 compound NcoI,fragments
R1435 T4969 T4959 punct ", ",fragments
R1436 T4970 T4971 advmod as,as
R1437 T4971 T4959 cc as,fragments
R1438 T4972 T4971 advmod well,as
R1439 T4973 T4974 nummod 7.2,kb
R1440 T4974 T4976 nmod kb,fragments
R1441 T4975 T4974 punct -,kb
R1442 T4976 T4959 conj fragments,fragments
R1443 T4977 T4978 amod wild,type
R1444 T4978 T4974 appos type,kb
R1445 T4979 T4978 punct -,type
R1446 T4980 T4974 cc and,kb
R1447 T4981 T4982 nummod 4.9,kb
R1448 T4982 T4984 compound kb,mutant
R1449 T4983 T4982 punct -,kb
R1450 T4984 T4974 conj mutant,kb
R1451 T4985 T4986 compound BglII,AgeI
R1452 T4986 T4976 compound AgeI,fragments
R1453 T4987 T4986 punct /,AgeI
R1454 T4988 T4989 punct (,shown
R1455 T4989 T4938 parataxis shown,detected
R1456 T4990 T4989 neg not,shown
R1457 T4991 T4989 punct ),shown
R1458 T4992 T4938 punct .,detected
R1459 T4994 T4995 det A,probe
R1460 T4995 T4996 nsubj probe,recognized
R1461 T4997 T4995 acl derived,probe
R1462 T4998 T4997 prep from,derived
R1463 T4999 T5000 det the,cassette
R1464 T5000 T4998 pobj cassette,from
R1465 T5001 T5002 compound PGK,Neo
R1466 T5002 T5000 compound Neo,cassette
R1467 T5003 T5002 punct -,Neo
R1468 T5004 T5005 advmod only,fragment
R1469 T5005 T4996 dobj fragment,recognized
R1470 T5006 T5005 det the,fragment
R1471 T5007 T5008 nummod 5.3,kb
R1472 T5008 T5005 compound kb,fragment
R1473 T5009 T5008 punct -,kb
R1474 T5010 T5005 compound BamHI,fragment
R1475 T5011 T4996 prep in,recognized
R1476 T5012 T5013 nmod Capn2,ES
R1477 T5013 T5017 compound ES,cells
R1478 T5014 T5013 punct +,ES
R1479 T5015 T5013 punct /,ES
R1480 T5016 T5013 punct -,ES
R1481 T5017 T5011 pobj cells,in
R1482 T5018 T4996 punct ", ",recognized
R1483 T5019 T4996 advcl suggesting,recognized
R1484 T5020 T5021 mark that,integrated
R1485 T5021 T5019 ccomp integrated,suggesting
R1486 T5022 T5023 det the,vector
R1487 T5023 T5021 nsubj vector,integrated
R1488 T5024 T5023 compound targeting,vector
R1489 T5025 T5021 aux had,integrated
R1490 T5026 T5027 advmod solely,at
R1491 T5027 T5021 prep at,integrated
R1492 T5028 T5029 det the,locus
R1493 T5029 T5027 pobj locus,at
R1494 T5030 T5029 compound Capn2,locus
R1495 T5031 T5032 punct (,shown
R1496 T5032 T5019 parataxis shown,suggesting
R1497 T5033 T5032 neg not,shown
R1498 T5034 T5032 punct ),shown
R1499 T5035 T4996 punct .,recognized
R1500 T5037 T5038 det A,method
R1501 T5038 T5041 nsubjpass method,established
R1502 T5039 T5038 compound PCR,method
R1503 T5040 T5038 compound screening,method
R1504 T5042 T5041 auxpass was,established
R1505 T5043 T5041 advmod also,established
R1506 T5044 T5045 dep that,generated
R1507 T5045 T5041 ccomp generated,established
R1508 T5046 T5047 det a,product
R1509 T5047 T5045 dobj product,generated
R1510 T5048 T5049 amod wild,type
R1511 T5049 T5047 compound type,product
R1512 T5050 T5049 punct -,type
R1513 T5051 T5047 prep of,product
R1514 T5052 T5053 nummod "2,749",bp
R1515 T5053 T5051 pobj bp,of
R1516 T5054 T5047 cc and,product
R1517 T5055 T5056 det a,product
R1518 T5056 T5047 conj product,product
R1519 T5057 T5058 nummod "2,711",bp
R1520 T5058 T5056 compound bp,product
R1521 T5059 T5056 prep from,product
R1522 T5060 T5061 det the,allele
R1523 T5061 T5059 pobj allele,from
R1524 T5062 T5061 compound mutant,allele
R1525 T5063 T5041 punct .,established
R1526 T5065 T5066 det The,product
R1527 T5066 T5069 nsubj product,was
R1528 T5067 T5068 nummod "2,711",bp
R1529 T5068 T5066 compound bp,product
R1530 T5070 T5069 advmod only,was
R1531 T5071 T5069 acomp evident,was
R1532 T5072 T5069 prep in,was
R1533 T5073 T5074 det the,lines
R1534 T5074 T5072 pobj lines,in
R1535 T5075 T5074 nummod two,lines
R1536 T5076 T5074 amod targeted,lines
R1537 T5077 T5074 compound cell,lines
R1538 T5078 T5079 punct (,2B
R1539 T5079 T5069 parataxis 2B,was
R1540 T5080 T5079 compound Figure,2B
R1541 T5081 T5079 punct ),2B
R1542 T5082 T5069 punct .,was
R1543 T6636 T6635 prep of,Generation
R1544 T6637 T6638 amod chimeric,mice
R1545 T6638 T6636 pobj mice,of
R1546 T6639 T6635 cc and,Generation
R1547 T6640 T6641 compound germline,transmission
R1548 T6641 T6635 conj transmission,Generation
R1549 T6642 T6641 prep of,transmission
R1550 T6643 T6644 det the,allele
R1551 T6644 T6642 pobj allele,of
R1552 T6645 T6644 compound mutant,allele
R1553 T6646 T6644 compound Capn2,allele
R1554 T6648 T6649 nummod Eight,mice
R1555 T6649 T6652 nsubjpass mice,produced
R1556 T6650 T6649 amod chimeric,mice
R1557 T6651 T6649 amod male,mice
R1558 T6653 T6652 auxpass were,produced
R1559 T6654 T6652 prep in,produced
R1560 T6655 T6656 compound morula,aggregation
R1561 T6656 T6657 compound aggregation,experiments
R1562 T6657 T6654 pobj experiments,in
R1563 T6658 T6652 advcl using,produced
R1564 T6659 T6660 det the,line
R1565 T6660 T6658 dobj line,using
R1566 T6661 T6660 nmod Capn2,line
R1567 T6662 T6661 punct +,Capn2
R1568 T6663 T6661 punct /,Capn2
R1569 T6664 T6661 punct -,Capn2
R1570 T6665 T6660 compound ES27,line
R1571 T6666 T6660 compound cell,line
R1572 T6667 T6652 punct .,produced
R1573 T6669 T6670 nsubj Two,transmitted
R1574 T6671 T6669 prep of,Two
R1575 T6672 T6673 det these,males
R1576 T6673 T6671 pobj males,of
R1577 T6674 T6675 det the,genotype
R1578 T6675 T6670 dobj genotype,transmitted
R1579 T6676 T6675 nmod Capn2,genotype
R1580 T6677 T6676 punct +,Capn2
R1581 T6678 T6676 punct /,Capn2
R1582 T6679 T6676 punct -,Capn2
R1583 T6680 T6675 compound ES27,genotype
R1584 T6681 T6670 prep through,transmitted
R1585 T6682 T6683 det the,germline
R1586 T6683 T6681 pobj germline,through
R1587 T6684 T6670 prep into,transmitted
R1588 T6685 T6686 det the,generation
R1589 T6686 T6684 pobj generation,into
R1590 T6687 T6686 compound F1,generation
R1591 T6688 T6670 punct .,transmitted
R1592 T6690 T6691 amod Heterozygous,animals
R1593 T6691 T6696 nsubj animals,appeared
R1594 T6692 T6691 nmod Capn2,animals
R1595 T6693 T6692 punct +,Capn2
R1596 T6694 T6692 punct /,Capn2
R1597 T6695 T6692 punct -,Capn2
R1598 T6697 T6696 oprd normal,appeared
R1599 T6698 T6696 punct ", ",appeared
R1600 T6699 T6696 prep with,appeared
R1601 T6700 T6701 det no,defects
R1602 T6701 T6699 pobj defects,with
R1603 T6702 T6701 amod obvious,defects
R1604 T6703 T6701 prep in,defects
R1605 T6704 T6705 amod gross,anatomy
R1606 T6705 T6703 pobj anatomy,in
R1607 T6706 T6705 punct ", ",anatomy
R1608 T6707 T6705 conj reproduction,anatomy
R1609 T6708 T6707 punct ", ",reproduction
R1610 T6709 T6707 cc or,reproduction
R1611 T6710 T6711 compound life,span
R1612 T6711 T6707 conj span,reproduction
R1613 T6712 T6696 punct .,appeared
R1614 T6714 T6715 prep Out,detected
R1615 T6716 T6714 prep of,Out
R1616 T6717 T6718 nummod 199,weanlings
R1617 T6718 T6716 pobj weanlings,of
R1618 T6719 T6718 prep from,weanlings
R1619 T6720 T6721 amod heterozygous,intercrosses
R1620 T6721 T6719 pobj intercrosses,from
R1621 T6722 T6715 punct ", ",detected
R1622 T6723 T6724 det no,progeny
R1623 T6724 T6715 nsubjpass progeny,detected
R1624 T6725 T6724 nmod Capn2,progeny
R1625 T6726 T6725 punct -,Capn2
R1626 T6727 T6725 punct /,Capn2
R1627 T6728 T6725 punct -,Capn2
R1628 T6729 T6715 auxpass were,detected
R1629 T6730 T6731 punct (,Table
R1630 T6731 T6715 parataxis Table,detected
R1631 T6732 T6731 nummod 2,Table
R1632 T6733 T6731 punct ),Table
R1633 T6734 T6715 punct .,detected
R1634 T6736 T6737 nsubj We,observe
R1635 T6738 T6737 aux did,observe
R1636 T6739 T6737 neg not,observe
R1637 T6740 T6741 amod high,rates
R1638 T6741 T6737 dobj rates,observe
R1639 T6742 T6741 prep of,rates
R1640 T6743 T6744 amod perinatal,death
R1641 T6744 T6742 pobj death,of
R1642 T6745 T6737 punct ", ",observe
R1643 T6746 T6737 cc and,observe
R1644 T6747 T6748 det no,stillborns
R1645 T6748 T6753 nsubjpass stillborns,observed
R1646 T6749 T6748 nmod Capn2,stillborns
R1647 T6750 T6749 punct -,Capn2
R1648 T6751 T6749 punct /,Capn2
R1649 T6752 T6749 punct -,Capn2
R1650 T6753 T6737 conj observed,observe
R1651 T6754 T6753 auxpass were,observed
R1652 T6755 T6753 punct .,observed
R1653 T6757 T6758 nsubj This,suggested
R1654 T6759 T6760 mark that,perished
R1655 T6760 T6758 ccomp perished,suggested
R1656 T6761 T6762 nmod Capn2,animals
R1657 T6762 T6760 nsubj animals,perished
R1658 T6763 T6761 punct -,Capn2
R1659 T6764 T6761 punct /,Capn2
R1660 T6765 T6761 punct -,Capn2
R1661 T6766 T6760 prep at,perished
R1662 T6767 T6768 det some,stage
R1663 T6768 T6766 pobj stage,at
R1664 T6769 T6768 prep during,stage
R1665 T6770 T6771 amod embryonic,development
R1666 T6771 T6769 pobj development,during
R1667 T6772 T6758 punct .,suggested
R1668 T6774 T6775 prep In,harvested
R1669 T6776 T6777 det an,attempt
R1670 T6777 T6774 pobj attempt,In
R1671 T6778 T6779 aux to,determine
R1672 T6779 T6777 acl determine,attempt
R1673 T6780 T6781 mark if,occurred
R1674 T6781 T6779 ccomp occurred,determine
R1675 T6782 T6783 amod embryonic,death
R1676 T6783 T6781 nsubj death,occurred
R1677 T6784 T6781 prep at,occurred
R1678 T6785 T6786 det a,stage
R1679 T6786 T6784 pobj stage,at
R1680 T6787 T6786 amod post-implantation,stage
R1681 T6788 T6775 punct ", ",harvested
R1682 T6789 T6775 nsubjpass embryos,harvested
R1683 T6790 T6775 auxpass were,harvested
R1684 T6791 T6775 prep for,harvested
R1685 T6792 T6791 pobj genotyping,for
R1686 T6793 T6775 prep at,harvested
R1687 T6794 T6795 amod different,times
R1688 T6795 T6793 pobj times,at
R1689 T6796 T6795 prep between,times
R1690 T6797 T6796 pobj E10.5,between
R1691 T6798 T6797 cc and,E10.5
R1692 T6799 T6797 conj E18.5,E10.5
R1693 T6800 T6775 punct .,harvested
R1694 T6802 T6803 det No,embryos
R1695 T6803 T6808 nsubjpass embryos,observed
R1696 T6804 T6803 nmod Capn2,embryos
R1697 T6805 T6804 punct -,Capn2
R1698 T6806 T6804 punct /,Capn2
R1699 T6807 T6804 punct -,Capn2
R1700 T6809 T6808 auxpass were,observed
R1701 T6810 T6808 cc and,observed
R1702 T6811 T6812 det no,signs
R1703 T6812 T6813 nsubjpass signs,detected
R1704 T6813 T6808 conj detected,observed
R1705 T6814 T6812 prep of,signs
R1706 T6815 T6816 compound embryo,resorption
R1707 T6816 T6814 pobj resorption,of
R1708 T6817 T6813 auxpass were,detected
R1709 T6818 T6819 punct (,Table
R1710 T6819 T6813 parataxis Table,detected
R1711 T6820 T6819 nummod 2,Table
R1712 T6821 T6819 punct ),Table
R1713 T6822 T6813 punct .,detected
R1714 T6824 T6825 nsubj This,indicated
R1715 T6826 T6827 mark that,dying
R1716 T6827 T6825 ccomp dying,indicated
R1717 T6828 T6829 det the,embryos
R1718 T6829 T6827 nsubj embryos,dying
R1719 T6830 T6829 nmod Capn2,embryos
R1720 T6831 T6830 punct -,Capn2
R1721 T6832 T6830 punct /,Capn2
R1722 T6833 T6830 punct -,Capn2
R1723 T6834 T6827 aux might,dying
R1724 T6835 T6827 aux be,dying
R1725 T6836 T6837 amod prior,to
R1726 T6837 T6827 prep to,dying
R1727 T6838 T6837 pobj implantation,to
R1728 T6839 T6825 punct .,indicated
R1729 T6841 T6842 nsubjpass Embryos,flushed
R1730 T6843 T6842 auxpass were,flushed
R1731 T6844 T6842 advmod then,flushed
R1732 T6845 T6842 prep from,flushed
R1733 T6846 T6847 det the,oviducts
R1734 T6847 T6845 pobj oviducts,from
R1735 T6848 T6847 prep of,oviducts
R1736 T6849 T6850 amod pregnant,females
R1737 T6850 T6848 pobj females,of
R1738 T6851 T6842 prep at,flushed
R1739 T6852 T6851 pobj E2.5,at
R1740 T6853 T6852 cc or,E2.5
R1741 T6854 T6852 conj E3.5,E2.5
R1742 T6855 T6842 punct ", ",flushed
R1743 T6856 T6842 cc and,flushed
R1744 T6857 T6842 conj genotyped,flushed
R1745 T6858 T6857 prep by,genotyped
R1746 T6859 T6858 pobj means,by
R1747 T6860 T6859 prep of,means
R1748 T6861 T6862 det a,strategy
R1749 T6862 T6860 pobj strategy,of
R1750 T6863 T6862 amod nested,strategy
R1751 T6864 T6862 compound PCR,strategy
R1752 T6865 T6866 punct (,Figure
R1753 T6866 T6857 parataxis Figure,genotyped
R1754 T6867 T6866 nummod 4,Figure
R1755 T6868 T6866 punct ),Figure
R1756 T6869 T6842 punct .,flushed
R1757 T6871 T6872 nsubj Two,were
R1758 T6873 T6871 prep of,Two
R1759 T6874 T6875 nummod 90,embryos
R1760 T6875 T6873 pobj embryos,of
R1761 T6876 T6877 advmod successfully,genotyped
R1762 T6877 T6875 amod genotyped,embryos
R1763 T6878 T6875 amod pre-implantation,embryos
R1764 T6879 T6872 attr Capn2,were
R1765 T6880 T6879 punct -,Capn2
R1766 T6881 T6879 punct /,Capn2
R1767 T6882 T6879 punct -,Capn2
R1768 T6883 T6872 punct ", ",were
R1769 T6884 T6885 punct (,Figure
R1770 T6885 T6872 parataxis Figure,were
R1771 T6886 T6885 dep Table,Figure
R1772 T6887 T6886 nummod 2,Table
R1773 T6888 T6885 punct ;,Figure
R1774 T6889 T6885 nummod 5,Figure
R1775 T6890 T6885 punct ),Figure
R1776 T6891 T6872 punct .,were
R1777 T6893 T6894 nsubjpass Both,isolated
R1778 T6895 T6893 prep of,Both
R1779 T6896 T6897 det these,embryos
R1780 T6897 T6895 pobj embryos,of
R1781 T6898 T6897 nmod Capn2,embryos
R1782 T6899 T6898 punct -,Capn2
R1783 T6900 T6898 punct /,Capn2
R1784 T6901 T6898 punct -,Capn2
R1785 T6902 T6894 auxpass were,isolated
R1786 T6903 T6894 prep at,isolated
R1787 T6904 T6905 det the,stage
R1788 T6905 T6903 pobj stage,at
R1789 T6906 T6907 nummod 8,cell
R1790 T6907 T6905 compound cell,stage
R1791 T6908 T6907 punct -,cell
R1792 T6909 T6894 cc and,isolated
R1793 T6910 T6911 aux did,display
R1794 T6911 T6894 conj display,isolated
R1795 T6912 T6911 neg not,display
R1796 T6913 T6914 det any,defects
R1797 T6914 T6911 dobj defects,display
R1798 T6915 T6914 amod obvious,defects
R1799 T6916 T6914 amod morphological,defects
R1800 T6917 T6894 punct .,isolated
R1802 T6921 T6919 prep of,None
R1803 T6922 T6923 det the,embryos
R1804 T6923 T6921 pobj embryos,of
R1805 T6924 T6923 nummod 46,embryos
R1806 T6925 T6926 advmod successfully,genotyped
R1807 T6926 T6923 amod genotyped,embryos
R1808 T6927 T6928 npadvmod blastocyst,staged
R1809 T6928 T6923 amod staged,embryos
R1810 T6929 T6928 punct -,staged
R1811 T6930 T6920 attr Capn2,were
R1812 T6931 T6930 punct -,Capn2
R1813 T6932 T6930 punct /,Capn2
R1814 T6933 T6930 punct -,Capn2
R1815 T6934 T6920 punct .,were
R1816 T6936 T6937 det The,scarcity
R1817 T6937 T6938 nsubj scarcity,suggested
R1818 T6939 T6937 prep of,scarcity
R1819 T6940 T6941 npadvmod Capn2,deficient
R1820 T6941 T6943 amod deficient,embryos
R1821 T6942 T6941 punct -,deficient
R1822 T6943 T6939 pobj embryos,of
R1823 T6944 T6943 acl surviving,embryos
R1824 T6945 T6944 prep to,surviving
R1825 T6946 T6947 det the,stage
R1826 T6947 T6945 pobj stage,to
R1827 T6948 T6949 nummod 8,cell
R1828 T6949 T6947 compound cell,stage
R1829 T6950 T6949 punct -,cell
R1830 T6951 T6952 mark that,compromise
R1831 T6952 T6938 ccomp compromise,suggested
R1832 T6953 T6954 det the,loss
R1833 T6954 T6952 nsubj loss,compromise
R1834 T6955 T6954 prep of,loss
R1835 T6956 T6957 compound m,calpain
R1836 T6957 T6959 compound calpain,activity
R1837 T6958 T6957 punct -,calpain
R1838 T6959 T6955 pobj activity,of
R1839 T6960 T6952 aux must,compromise
R1840 T6961 T6952 advmod fatally,compromise
R1841 T6962 T6963 det the,viability
R1842 T6963 T6952 dobj viability,compromise
R1843 T6964 T6963 prep of,viability
R1844 T6965 T6966 amod early,embryos
R1845 T6966 T6964 pobj embryos,of
R1846 T6967 T6938 punct .,suggested
R1847 T6969 T6970 advmod Furthermore,is
R1848 T6971 T6970 punct ", ",is
R1849 T6972 T6970 nsubj it,is
R1850 T6973 T6970 acomp possible,is
R1851 T6974 T6975 mark that,allowed
R1852 T6975 T6970 ccomp allowed,is
R1853 T6976 T6975 nsubj persistence,allowed
R1854 T6977 T6976 prep of,persistence
R1855 T6978 T6979 det some,transcript
R1856 T6979 T6977 pobj transcript,of
R1857 T6980 T6981 advmod maternally,derived
R1858 T6981 T6979 amod derived,transcript
R1859 T6982 T6979 compound mRNA,transcript
R1860 T6983 T6979 cc or,transcript
R1861 T6984 T6979 conj protein,transcript
R1862 T6985 T6975 aux might,allowed
R1863 T6986 T6975 aux have,allowed
R1864 T6987 T6988 det a,number
R1865 T6988 T6990 nsubj number,survive
R1866 T6989 T6988 amod small,number
R1867 T6990 T6975 ccomp survive,allowed
R1868 T6991 T6988 prep of,number
R1869 T6992 T6993 nmod Capn2,embryos
R1870 T6993 T6991 pobj embryos,of
R1871 T6994 T6992 punct -,Capn2
R1872 T6995 T6992 punct /,Capn2
R1873 T6996 T6992 punct -,Capn2
R1874 T6997 T6990 aux to,survive
R1875 T6998 T6990 prep to,survive
R1876 T6999 T7000 det the,stage
R1877 T7000 T6998 pobj stage,to
R1878 T7001 T7000 compound morula,stage
R1879 T7002 T7000 punct -,stage
R1880 T7003 T6970 punct .,is
R1892 T7017 T7007 auxpass are,shown
R1893 T7018 T7007 prep in,shown
R1894 T7019 T7018 pobj Table,in
R1895 T7020 T7019 nummod 2,Table
R1896 T7021 T7007 punct .,shown
R1897 T7023 T7024 advmod Curiously,was
R1898 T7025 T7024 punct ", ",was
R1899 T7026 T7027 det the,ratio
R1900 T7027 T7024 nsubj ratio,was
R1901 T7028 T7027 prep of,ratio
R1902 T7029 T7028 pobj Capn2,of
R1903 T7030 T7029 punct +,Capn2
R1904 T7031 T7029 punct /,Capn2
R1905 T7032 T7029 punct +,Capn2
R1906 T7033 T7027 prep to,ratio
R1907 T7034 T7035 nmod Capn2,animals
R1908 T7035 T7033 pobj animals,to
R1909 T7036 T7034 punct +,Capn2
R1910 T7037 T7034 punct /,Capn2
R1911 T7038 T7034 punct -,Capn2
R1912 T7039 T7027 prep from,ratio
R1913 T7040 T7041 amod inbred,intercrosses
R1914 T7041 T7039 pobj intercrosses,from
R1915 T7042 T7041 amod heterozygous,intercrosses
R1916 T7043 T7044 advmod substantially,less
R1917 T7044 T7024 acomp less,was
R1918 T7045 T7044 prep than,less
R1919 T7046 T7047 det the,ratio
R1920 T7047 T7045 pobj ratio,than
R1921 T7048 T7047 amod predicted,ratio
R1922 T7049 T7047 nummod 1,ratio
R1923 T7050 T7051 punct :,2
R1924 T7051 T7049 prep 2,1
R1925 T7052 T7047 amod Mendelian,ratio
R1926 T7053 T7024 punct .,was
R1927 T7055 T7056 prep In,observed
R1928 T7057 T7058 det a,group
R1929 T7058 T7055 pobj group,In
R1930 T7059 T7058 prep of,group
R1931 T7060 T7061 nummod 199,animals
R1932 T7061 T7059 pobj animals,of
R1933 T7062 T7061 acl derived,animals
R1934 T7063 T7062 prep from,derived
R1935 T7064 T7065 compound heterozygote,breeding
R1936 T7065 T7063 pobj breeding,from
R1937 T7066 T7067 punct (,litters
R1938 T7067 T7065 parataxis litters,breeding
R1939 T7068 T7067 nummod 33,litters
R1940 T7069 T7067 punct ),litters
R1941 T7070 T7056 punct ", ",observed
R1942 T7071 T7072 nummod 23,Capn2
R1943 T7072 T7056 nsubjpass Capn2,observed
R1944 T7073 T7072 punct +,Capn2
R1945 T7074 T7072 punct /,Capn2
R1946 T7075 T7072 punct +,Capn2
R1947 T7076 T7077 punct (,%
R1948 T7077 T7072 parataxis %,Capn2
R1949 T7078 T7077 nummod 11.6,%
R1950 T7079 T7077 punct ),%
R1951 T7080 T7072 cc and,Capn2
R1952 T7081 T7082 nummod 176,Capn2
R1953 T7082 T7087 nmod Capn2,animals
R1954 T7083 T7084 punct (,%
R1955 T7084 T7082 parataxis %,Capn2
R1956 T7085 T7084 nummod 88.4,%
R1957 T7086 T7084 punct ),%
R1958 T7087 T7072 conj animals,Capn2
R1959 T7088 T7082 punct +,Capn2
R1960 T7089 T7082 punct /,Capn2
R1961 T7090 T7082 punct -,Capn2
R1962 T7091 T7056 auxpass were,observed
R1963 T7092 T7056 punct .,observed
R1964 T7094 T7095 det The,ratio
R1965 T7095 T7096 nsubj ratio,was
R1966 T7097 T7095 prep of,ratio
R1967 T7098 T7097 pobj Capn2,of
R1968 T7099 T7098 punct +,Capn2
R1969 T7100 T7098 punct /,Capn2
R1970 T7101 T7098 punct +,Capn2
R1971 T7102 T7095 prep to,ratio
R1972 T7103 T7104 nmod Capn2,animals
R1973 T7104 T7102 pobj animals,to
R1974 T7105 T7103 punct +,Capn2
R1975 T7106 T7103 punct /,Capn2
R1976 T7107 T7103 punct -,Capn2
R1977 T7108 T7095 prep among,ratio
R1978 T7109 T7108 pobj males,among
R1979 T7110 T7111 punct (,%
R1980 T7111 T7109 parataxis %,males
R1981 T7112 T7111 nummod 14,%
R1982 T7113 T7111 prep to,%
R1983 T7114 T7115 nummod 82,%
R1984 T7115 T7113 pobj %,to
R1985 T7116 T7111 punct ),%
R1986 T7117 T7109 cc or,males
R1987 T7118 T7109 conj females,males
R1988 T7202 T7183 advcl was,reported
R1989 T7119 T7120 punct (,%
R1990 T7203 T7204 det the,skewing
R1991 T7120 T7118 parataxis %,females
R1992 T7204 T7202 nsubj skewing,was
R1993 T7121 T7120 nummod 13,%
R1994 T7205 T7204 compound genotype,skewing
R1995 T7206 T7202 neg not,was
R1996 T7207 T7202 prep as,was
R1997 T7122 T7120 prep to,%
R1998 T7208 T7207 amod extreme,as
R1999 T7209 T7210 punct [,32
R2000 T7123 T7124 nummod 90,%
R2001 T7210 T7202 parataxis 32,was
R2002 T7211 T7210 punct ],32
R2003 T7212 T7183 punct .,reported
R2004 T7124 T7122 pobj %,to
R2005 T7214 T7215 nsubj Crosses,resulted
R2006 T7125 T7120 punct ),%
R2007 T7216 T7214 prep between,Crosses
R2008 T7217 T7218 nmod Capn2,animals
R2009 T7218 T7216 pobj animals,between
R2010 T7219 T7217 punct +,Capn2
R2011 T7220 T7217 punct /,Capn2
R2012 T7126 T7096 advmod essentially,was
R2013 T7221 T7217 punct +,Capn2
R2014 T7222 T7217 cc and,Capn2
R2015 T7223 T7217 conj Capn2,Capn2
R2016 T7224 T7223 punct +,Capn2
R2017 T7127 T7128 advmod the,same
R2018 T7128 T7096 acomp same,was
R2019 T7129 T7130 mark is,was
R2020 T7225 T7223 punct /,Capn2
R2021 T7130 T7128 advcl was,same
R2022 T7226 T7223 punct -,Capn2
R2023 T7227 T7215 advmod also,resulted
R2024 T7228 T7215 prep in,resulted
R2025 T7131 T7130 nsubj it,was
R2026 T7229 T7230 det a,proportion
R2027 T7230 T7228 pobj proportion,in
R2028 T7132 T7130 prep for,was
R2029 T7231 T7230 amod greater,proportion
R2030 T7232 T7233 mark than,expected
R2031 T7233 T7231 advcl expected,greater
R2032 T7133 T7134 det the,population
R2033 T7234 T7230 prep of,proportion
R2034 T7235 T7236 nmod Capn2,animals
R2035 T7134 T7132 pobj population,for
R2036 T7236 T7234 pobj animals,of
R2037 T7237 T7235 punct +,Capn2
R2038 T7238 T7235 punct /,Capn2
R2039 T7135 T7134 amod combined,population
R2040 T7239 T7235 punct -,Capn2
R2041 T7240 T7241 punct (,Table
R2042 T7241 T7215 parataxis Table,resulted
R2043 T7136 T7096 punct ", ",was
R2044 T7242 T7241 nummod 2,Table
R2045 T7243 T7241 punct ),Table
R2046 T7244 T7215 punct .,resulted
R2047 T7137 T7096 cc and,was
R2048 T7246 T7247 det An,degree
R2049 T7247 T7250 nsubjpass degree,seen
R2050 T7138 T7139 expl there,were
R2051 T7248 T7249 advmod even,higher
R2052 T7249 T7247 amod higher,degree
R2053 T7139 T7096 conj were,was
R2054 T7251 T7247 prep of,degree
R2055 T7252 T7253 nmod Capn2,over-representation
R2056 T7140 T7141 det an,average
R2057 T7253 T7251 pobj over-representation,of
R2058 T7254 T7252 punct +,Capn2
R2059 T7255 T7252 punct /,Capn2
R2060 T7141 T7139 attr average,were
R2061 T7256 T7252 punct -,Capn2
R2062 T7257 T7250 auxpass was,seen
R2063 T7258 T7259 advmod when,came
R2064 T7259 T7250 advcl came,seen
R2065 T7260 T7261 det the,allele
R2066 T7261 T7259 nsubj allele,came
R2067 T7142 T7141 prep of,average
R2068 T7262 T7261 compound mutant,allele
R2069 T7263 T7264 det the,mother
R2070 T7264 T7259 dobj mother,came
R2071 T7143 T7144 nummod six,pups
R2072 T7265 T7266 punct (,%
R2073 T7266 T7259 parataxis %,came
R2074 T7144 T7142 pobj pups,of
R2075 T7267 T7266 nummod 73,%
R2076 T7268 T7266 punct ),%
R2077 T7269 T7250 prep compared,seen
R2078 T7145 T7144 prep per,pups
R2079 T7270 T7269 prep to,compared
R2080 T7271 T7272 advmod when,came
R2081 T7146 T7145 pobj litter,per
R2082 T7272 T7270 pcomp came,to
R2083 T7273 T7272 nsubj it,came
R2084 T7147 T7141 punct ", ",average
R2085 T7148 T7149 dep which,is
R2086 T7274 T7272 prep from,came
R2087 T7149 T7141 relcl is,average
R2088 T7275 T7276 det the,father
R2089 T7276 T7274 pobj father,from
R2090 T7150 T7149 acomp normal,is
R2091 T7277 T7278 punct (,%
R2092 T7278 T7272 parataxis %,came
R2093 T7279 T7278 nummod 59,%
R2094 T7151 T7149 prep for,is
R2095 T7280 T7278 punct ),%
R2096 T7281 T7250 punct .,seen
R2097 T7152 T7153 det this,strain
R2098 T7283 T7284 prep In,compared
R2099 T7153 T7151 pobj strain,for
R2100 T7285 T7286 det these,crosses
R2101 T7286 T7283 pobj crosses,In
R2102 T7154 T7153 compound background,strain
R2103 T7287 T7288 det the,ratios
R2104 T7288 T7284 nsubj ratios,compared
R2105 T7289 T7288 prep of,ratios
R2106 T7155 T7139 punct .,were
R2107 T7290 T7289 pobj Capn2,of
R2108 T7291 T7290 punct +,Capn2
R2109 T7157 T7158 det A,proportion
R2110 T7292 T7290 punct /,Capn2
R2111 T7293 T7290 punct +,Capn2
R2112 T7294 T7288 prep to,ratios
R2113 T7295 T7296 nmod Capn2,animals
R2114 T7296 T7294 pobj animals,to
R2115 T7297 T7295 punct +,Capn2
R2116 T7298 T7295 punct /,Capn2
R2117 T7158 T7162 nsubj proportion,was
R2118 T7299 T7295 punct -,Capn2
R2119 T7300 T7288 prep among,ratios
R2120 T7301 T7300 pobj males,among
R2121 T7159 T7158 amod larger,proportion
R2122 T7302 T7301 cc or,males
R2123 T7303 T7301 conj females,males
R2124 T7304 T7284 advmod well,compared
R2125 T7160 T7161 mark than,expected
R2126 T7305 T7284 prep with,compared
R2127 T7306 T7307 det the,ratio
R2128 T7161 T7159 advcl expected,larger
R2129 T7163 T7164 nmod Capn2,animals
R2130 T7164 T7158 npadvmod animals,proportion
R2131 T7307 T7305 pobj ratio,with
R2132 T7165 T7163 punct +,Capn2
R2133 T7308 T7284 prep in,compared
R2134 T7309 T7310 det the,populations
R2135 T7310 T7308 pobj populations,in
R2136 T7166 T7163 punct /,Capn2
R2137 T7311 T7310 amod combined,populations
R2138 T7312 T7284 punct ;,compared
R2139 T7167 T7163 punct -,Capn2
R2140 T7313 T7314 nummod 77,%
R2141 T7314 T7315 nsubj %,were
R2142 T7315 T7284 conj were,compared
R2143 T7168 T7162 advmod also,was
R2144 T7316 T7314 prep of,%
R2145 T7317 T7316 pobj males,of
R2146 T7318 T7314 cc and,%
R2147 T7169 T7162 acomp evident,was
R2148 T7319 T7320 nummod 69,%
R2149 T7320 T7314 conj %,%
R2150 T7170 T7162 prep in,was
R2151 T7321 T7320 prep of,%
R2152 T7322 T7321 pobj females,of
R2153 T7323 T7315 attr Capn2,were
R2154 T7171 T7172 preconj both,embryos
R2155 T7324 T7323 punct +,Capn2
R2156 T7325 T7323 punct /,Capn2
R2157 T7326 T7323 punct -,Capn2
R2158 T7172 T7170 pobj embryos,in
R2159 T7327 T7328 advmod when,came
R2160 T7328 T7315 advcl came,were
R2161 T7329 T7330 det the,allele
R2162 T7173 T7172 nmod post-implantation,embryos
R2163 T7330 T7328 nsubj allele,came
R2164 T7331 T7330 compound mutant,allele
R2165 T7332 T7328 prep from,came
R2166 T7333 T7334 det the,mother
R2167 T7334 T7332 pobj mother,from
R2168 T7335 T7315 punct ", ",were
R2169 T7174 T7173 cc and,post-implantation
R2170 T7336 T7315 cc and,were
R2171 T7337 T7338 nummod 62,%
R2172 T7338 T7339 nsubj %,were
R2173 T7175 T7173 conj pre-implantation,post-implantation
R2174 T7339 T7315 conj were,were
R2175 T7340 T7338 prep of,%
R2176 T7341 T7340 pobj males,of
R2177 T7176 T7177 punct (,Table
R2178 T7342 T7338 cc and,%
R2179 T7343 T7344 nummod 55,%
R2180 T7344 T7338 conj %,%
R2181 T7177 T7162 parataxis Table,was
R2182 T7345 T7344 prep of,%
R2183 T7346 T7345 pobj females,of
R2184 T7347 T7339 attr Capn2,were
R2185 T7178 T7177 nummod 2,Table
R2186 T7348 T7347 punct +,Capn2
R2187 T7349 T7347 punct /,Capn2
R2188 T7350 T7347 punct -,Capn2
R2189 T7179 T7177 punct ),Table
R2190 T7351 T7352 advmod when,came
R2191 T7352 T7339 advcl came,were
R2192 T7180 T7162 punct .,was
R2193 T7353 T7354 det the,allele
R2194 T7354 T7352 nsubj allele,came
R2195 T7355 T7354 compound mutant,allele
R2196 T7182 T7183 advmod Interestingly,reported
R2197 T7356 T7352 prep from,came
R2198 T7184 T7183 punct ", ",reported
R2199 T7357 T7358 det the,father
R2200 T7358 T7356 pobj father,from
R2201 T7185 T7186 det a,over-representation
R2202 T7359 T7339 punct .,were
R2203 T7186 T7183 nsubjpass over-representation,reported
R2204 T7187 T7186 amod similar,over-representation
R2205 T7188 T7186 prep of,over-representation
R2206 T7189 T7190 amod heterozygous,animals
R2207 T7190 T7188 pobj animals,of
R2208 T7191 T7183 auxpass was,reported
R2209 T7192 T7183 advmod also,reported
R2210 T7193 T7183 prep in,reported
R2211 T7194 T7193 pobj one,in
R2212 T7195 T7194 prep of,one
R2213 T7196 T7197 det the,lines
R2214 T7197 T7195 pobj lines,of
R2215 T7198 T7197 nmod Capn4,lines
R2216 T7199 T7197 amod transgenic,lines
R2217 T7200 T7183 punct ", ",reported
R2218 T7201 T7202 mark though,was
R2221 T7704 T7705 amod Attempted,generation
R2222 T7706 T7705 prep of,generation
R2223 T7707 T7708 nmod Capn2,cells
R2224 T7708 T7706 pobj cells,of
R2225 T7709 T7707 punct -,Capn2
R2226 T7710 T7707 punct /,Capn2
R2227 T7711 T7707 punct -,Capn2
R2228 T7712 T7708 compound ES,cells
R2229 T7714 T7715 nmod Capn2,cells
R2230 T7715 T7720 nsubjpass cells,subjected
R2231 T7716 T7714 punct +,Capn2
R2232 T7717 T7714 punct /,Capn2
R2233 T7718 T7714 punct -,Capn2
R2234 T7719 T7715 compound ES,cells
R2235 T7721 T7720 auxpass were,subjected
R2236 T7722 T7720 prep to,subjected
R2237 T7723 T7724 amod clonal,selection
R2238 T7724 T7722 pobj selection,to
R2239 T7725 T7720 prep in,subjected
R2240 T7726 T7727 det the,presence
R2241 T7727 T7725 pobj presence,in
R2242 T7728 T7727 prep of,presence
R2243 T7729 T7730 nummod 2,mg
R2244 T7730 T7731 nmod mg,G418
R2245 T7731 T7728 pobj G418,of
R2246 T7732 T7733 punct /,mL
R2247 T7733 T7730 prep mL,mg
R2248 T7734 T7727 prep in,presence
R2249 T7735 T7734 pobj attempts,in
R2250 T7736 T7737 aux to,generate
R2251 T7737 T7735 acl generate,attempts
R2252 T7738 T7739 amod homozygous,cells
R2253 T7739 T7737 dobj cells,generate
R2254 T7740 T7739 compound mutant,cells
R2255 T7741 T7737 prep by,generate
R2256 T7742 T7743 compound gene,conversion
R2257 T7743 T7741 pobj conversion,by
R2258 T7744 T7720 punct .,subjected
R2259 T7746 T7747 det This,procedure
R2260 T7747 T7748 nsubjpass procedure,applied
R2261 T7749 T7748 aux has,applied
R2262 T7750 T7748 auxpass been,applied
R2263 T7751 T7748 advmod extensively,applied
R2264 T7752 T7748 prep to,applied
R2265 T7753 T7754 amod targeted,cells
R2266 T7754 T7752 pobj cells,to
R2267 T7755 T7754 compound ES,cells
R2268 T7756 T7748 cc and,applied
R2269 T7757 T7758 auxpass was,achieved
R2270 T7758 T7748 conj achieved,applied
R2271 T7759 T7758 advmod readily,achieved
R2272 T7760 T7758 prep in,achieved
R2273 T7761 T7762 det the,case
R2274 T7762 T7760 pobj case,in
R2275 T7763 T7762 prep of,case
R2276 T7764 T7765 nmod Capn4,cells
R2277 T7765 T7763 pobj cells,of
R2278 T7766 T7764 punct +,Capn4
R2279 T7767 T7764 punct /,Capn4
R2280 T7768 T7764 punct -,Capn4
R2281 T7769 T7765 compound ES,cells
R2282 T7770 T7771 punct [,31
R2283 T7771 T7758 parataxis 31,achieved
R2284 T7772 T7771 punct ],31
R2285 T7773 T7748 punct .,applied
R2286 T7775 T7776 prep In,isolated
R2287 T7777 T7778 det this,case
R2288 T7778 T7775 pobj case,In
R2289 T7779 T7776 punct ", ",isolated
R2290 T7780 T7776 advmod however,isolated
R2291 T7781 T7776 punct ", ",isolated
R2292 T7782 T7783 det no,cells
R2293 T7783 T7776 nsubjpass cells,isolated
R2294 T7784 T7783 nmod Capn2,cells
R2295 T7785 T7784 punct -,Capn2
R2296 T7786 T7784 punct /,Capn2
R2297 T7787 T7784 punct -,Capn2
R2298 T7788 T7783 compound ES,cells
R2299 T7789 T7776 auxpass were,isolated
R2300 T7790 T7776 prep in,isolated
R2301 T7791 T7790 pobj screens,in
R2302 T7792 T7791 prep of,screens
R2303 T7793 T7794 nummod 126,clones
R2304 T7794 T7792 pobj clones,of
R2305 T7795 T7796 npadvmod drug,resistant
R2306 T7796 T7794 amod resistant,clones
R2307 T7797 T7796 punct -,resistant
R2308 T7798 T7776 punct .,isolated
R2309 T7800 T7801 det The,inability
R2310 T7801 T7802 nsubj inability,suggested
R2311 T7803 T7804 aux to,isolate
R2312 T7804 T7801 acl isolate,inability
R2313 T7805 T7806 nmod Capn2,cells
R2314 T7806 T7804 dobj cells,isolate
R2315 T7807 T7805 punct -,Capn2
R2316 T7808 T7805 punct /,Capn2
R2317 T7809 T7805 punct -,Capn2
R2318 T7810 T7806 compound ES,cells
R2319 T7811 T7804 punct ", ",isolate
R2320 T7812 T7804 prep in,isolate
R2321 T7813 T7812 pobj concert,in
R2322 T7814 T7813 prep with,concert
R2323 T7815 T7816 det the,absence
R2324 T7816 T7814 pobj absence,with
R2325 T7817 T7816 prep of,absence
R2326 T7818 T7819 nmod Capn2,embryos
R2327 T7819 T7817 pobj embryos,of
R2328 T7820 T7818 punct -,Capn2
R2329 T7821 T7818 punct /,Capn2
R2330 T7822 T7818 punct -,Capn2
R2331 T7823 T7816 prep beyond,absence
R2332 T7824 T7825 det the,stage
R2333 T7825 T7823 pobj stage,beyond
R2334 T7826 T7827 nummod 8,cell
R2335 T7827 T7825 compound cell,stage
R2336 T7828 T7827 punct -,cell
R2337 T7829 T7802 punct ", ",suggested
R2338 T7830 T7831 mark that,be
R2339 T7831 T7802 ccomp be,suggested
R2340 T7832 T7833 compound m,calpain
R2341 T7833 T7835 compound calpain,activity
R2342 T7834 T7833 punct -,calpain
R2343 T7835 T7831 nsubj activity,be
R2344 T7836 T7831 aux might,be
R2345 T7837 T7831 acomp essential,be
R2346 T7838 T7837 prep for,essential
R2347 T7839 T7840 compound cell,viability
R2348 T7840 T7838 pobj viability,for
R2349 T7841 T7840 cc or,viability
R2350 T7842 T7843 det the,establishment
R2351 T7843 T7840 conj establishment,viability
R2352 T7844 T7843 prep of,establishment
R2353 T7845 T7846 amod viable,clones
R2354 T7846 T7844 pobj clones,of
R2355 T7847 T7846 compound ES,clones
R2356 T7848 T7846 compound cell,clones
R2357 T7849 T7802 punct .,suggested
R2370 T12875 T12876 det the,story
R2373 T12816 T12817 mark Although,identified
R2374 T12876 T12870 nsubjpass story,complicated
R2375 T12877 T12870 aux has,complicated
R2376 T12817 T12822 advcl identified,remained
R2377 T12818 T12819 compound calpain,activity
R2378 T12878 T12870 auxpass been,complicated
R2379 T12819 T12817 nsubjpass activity,identified
R2380 T12820 T12817 auxpass was,identified
R2381 T12821 T12817 advmod first,identified
R2382 T12879 T12870 advmod further,complicated
R2383 T12823 T12824 nummod four,decades
R2384 T12880 T12870 agent by,complicated
R2385 T12824 T12825 npadvmod decades,ago
R2386 T12825 T12817 advmod ago,identified
R2387 T12826 T12822 punct ", ",remained
R2388 T12881 T12882 det the,discovery
R2389 T12827 T12828 det a,understanding
R2390 T12828 T12822 nsubj understanding,remained
R2391 T12882 T12880 pobj discovery,by
R2392 T12829 T12828 amod clear,understanding
R2393 T12830 T12828 prep of,understanding
R2394 T12831 T12832 det the,substrates
R2395 T12883 T12882 prep of,discovery
R2396 T12884 T12885 det a,number
R2397 T12832 T12830 pobj substrates,of
R2398 T12885 T12883 pobj number,of
R2399 T12833 T12832 cc and,substrates
R2400 T12834 T12832 conj functions,substrates
R2401 T12886 T12885 prep of,number
R2402 T12835 T12832 prep of,substrates
R2403 T12836 T12837 det the,enzymes
R2404 T12887 T12888 advmod previously,unknown
R2405 T12837 T12835 pobj enzymes,of
R2406 T12838 T12822 aux has,remained
R2407 T12839 T12822 acomp elusive,remained
R2408 T12840 T12822 punct .,remained
R2409 T12842 T12843 prep In,been
R2410 T12844 T12845 amod large,part
R2411 T12845 T12842 pobj part,In
R2412 T12846 T12843 punct ", ",been
R2413 T12847 T12843 nsubj this,been
R2414 T12848 T12843 aux has,been
R2415 T12849 T12843 prep due,been
R2416 T12850 T12849 pcomp to,due
R2417 T12888 T12889 amod unknown,isoforms
R2418 T12851 T12852 det the,lack
R2419 T12852 T12849 pobj lack,due
R2420 T12853 T12852 prep of,lack
R2421 T12854 T12853 pobj inhibitors,of
R2422 T12855 T12854 amod capable,inhibitors
R2423 T12856 T12855 prep of,capable
R2424 T12889 T12886 pobj isoforms,of
R2425 T12857 T12858 advmod specifically,regulating
R2426 T12858 T12856 pcomp regulating,of
R2427 T12859 T12858 advmod down,regulating
R2428 T12890 T12891 dep which,regulated
R2429 T12860 T12858 punct -,regulating
R2430 T12861 T12862 det the,calpains
R2431 T12862 T12858 dobj calpains,regulating
R2432 T12891 T12889 relcl regulated,isoforms
R2433 T12863 T12858 prep without,regulating
R2434 T12864 T12863 pcomp affecting,without
R2435 T12865 T12866 amod other,proteases
R2436 T12892 T12891 aux may,regulated
R2437 T12866 T12864 dobj proteases,affecting
R2438 T12867 T12843 punct .,been
R2439 T12893 T12891 auxpass be,regulated
R2440 T12869 T12870 prep In,complicated
R2441 T12894 T12891 advmod differently,regulated
R2442 T12871 T12872 det the,decade
R2443 T12872 T12869 pobj decade,In
R2444 T12873 T12872 amod past,decade
R2445 T12895 T12891 cc and,regulated
R2446 T12874 T12870 punct ", ",complicated
R2447 T12896 T12891 conj have,regulated
R2448 T12897 T12898 amod different,specificity
R2449 T12898 T12896 dobj specificity,have
R2450 T12899 T12898 compound substrate,specificity
R2451 T12981 T12979 nsubjpass subunit,required
R2452 T12900 T12870 punct .,complicated
R2453 T12982 T12981 amod small,subunit
R2454 T12983 T12979 auxpass is,required
R2455 T12902 T12903 compound Gene,targeting
R2456 T12984 T12979 prep for,required
R2457 T12985 T12986 preconj both,calpain
R2458 T12903 T12904 nsubj targeting,provided
R2459 T12905 T12903 prep in,targeting
R2460 T12986 T12984 pobj calpain,for
R2461 T12987 T12986 nmod μ,calpain
R2462 T12988 T12987 punct -,μ
R2463 T12989 T12987 cc and,μ
R2464 T12990 T12987 conj m,μ
R2465 T12991 T12986 punct -,calpain
R2466 T12992 T12974 punct ", ",supported
R2467 T12993 T12974 cc and,supported
R2468 T12994 T12995 advmod furthermore,suggested
R2469 T12906 T12905 pobj mice,in
R2470 T12995 T12974 conj suggested,supported
R2471 T12996 T12997 nummod four,possibilities
R2472 T12997 T12995 dobj possibilities,suggested
R2473 T12907 T12904 aux has,provided
R2474 T12998 T12997 prep regarding,possibilities
R2475 T12999 T13000 poss their,requirement
R2476 T13000 T12998 pobj requirement,regarding
R2477 T12908 T12909 det a,approach
R2478 T13001 T13000 prep for,requirement
R2479 T13002 T13003 amod embryonic,development
R2480 T13003 T13001 pobj development,for
R2481 T12909 T12904 dobj approach,provided
R2482 T13004 T12975 punct : ,required
R2483 T13005 T12975 meta 1,required
R2484 T13006 T13005 punct ),1
R2485 T12910 T12909 amod powerful,approach
R2486 T13007 T13008 det both,isoforms
R2487 T13008 T12975 nsubjpass isoforms,required
R2488 T12911 T12912 aux to,examine
R2489 T13009 T12975 auxpass were,required
R2490 T13010 T12975 punct ;,required
R2491 T13011 T13012 meta 2,required
R2492 T13012 T12975 conj required,required
R2493 T13013 T13011 punct ),2
R2494 T12912 T12909 advcl examine,approach
R2495 T13014 T13015 compound μ,calpain
R2496 T13015 T13012 nsubjpass calpain,required
R2497 T13016 T13015 punct -,calpain
R2498 T12913 T12914 det the,roles
R2499 T13017 T13012 auxpass was,required
R2500 T13018 T13012 punct ;,required
R2501 T13019 T13020 meta 3,required
R2502 T12914 T12912 dobj roles,examine
R2503 T13020 T13012 conj required,required
R2504 T13021 T13019 punct ),3
R2505 T13022 T13023 compound m,calpain
R2506 T12915 T12914 amod physiologic,roles
R2507 T13023 T13020 nsubjpass calpain,required
R2508 T13024 T13023 punct -,calpain
R2509 T13025 T13020 auxpass was,required
R2510 T12916 T12914 prep of,roles
R2511 T13026 T13020 punct ", ",required
R2512 T13027 T13020 cc or,required
R2513 T13028 T13029 meta 4,are
R2514 T13029 T13020 conj are,required
R2515 T13030 T13028 punct ),4
R2516 T13031 T13032 nmod μ,calpain
R2517 T13032 T13029 nsubj calpain,are
R2518 T13033 T13031 punct -,μ
R2519 T12917 T12918 amod individual,isoforms
R2520 T13034 T13031 cc and,μ
R2521 T13035 T13031 conj m,μ
R2522 T13036 T13032 punct -,calpain
R2523 T12918 T12916 pobj isoforms,of
R2524 T13037 T13029 acomp redundant,are
R2525 T13038 T13029 punct ", ",are
R2526 T13039 T13029 cc and,are
R2527 T12919 T12918 compound calpain,isoforms
R2528 T13040 T13041 nsubjpass one,required
R2529 T13041 T13029 conj required,are
R2530 T13042 T13040 cc or,one
R2531 T12920 T12904 punct .,provided
R2532 T13043 T13044 det the,other
R2533 T13044 T13045 nmod other,isoform
R2534 T13045 T13040 conj isoform,one
R2535 T12922 T12923 nsubjpass This,used
R2536 T13046 T13041 auxpass was,required
R2537 T13047 T12975 punct .,required
R2538 T13049 T13050 det These,options
R2539 T12924 T12923 auxpass was,used
R2540 T13050 T13051 nsubjpass options,narrowed
R2541 T13052 T13051 auxpass were,narrowed
R2542 T13053 T13051 prt down,narrowed
R2543 T13054 T13051 agent by,narrowed
R2544 T12925 T12923 advmod first,used
R2545 T13055 T13056 det the,observation
R2546 T13056 T13054 pobj observation,by
R2547 T13057 T13056 amod subsequent,observation
R2548 T13058 T13059 mark that,were
R2549 T12926 T12927 aux to,disrupt
R2550 T12927 T12923 advcl disrupt,used
R2551 T13059 T13056 acl were,observation
R2552 T12928 T12929 det the,gene
R2553 T13060 T13061 nmod Capn1,mice
R2554 T13061 T13059 nsubj mice,were
R2555 T13062 T13060 punct -,Capn1
R2556 T13063 T13060 punct /,Capn1
R2557 T12929 T12927 dobj gene,disrupt
R2558 T13064 T13060 punct -,Capn1
R2559 T13065 T13061 punct ", ",mice
R2560 T12930 T12929 compound Capn4,gene
R2561 T13066 T13067 dep which,lack
R2562 T13067 T13061 relcl lack,mice
R2563 T13068 T13069 det the,subunit
R2564 T13069 T13067 dobj subunit,lack
R2565 T13070 T13071 nmod μ,calpain
R2566 T13071 T13069 nmod calpain,subunit
R2567 T13072 T13071 punct -,calpain
R2568 T13073 T13069 amod catalytic,subunit
R2569 T13074 T13059 punct ", ",were
R2570 T13075 T13059 acomp healthy,were
R2571 T13076 T13075 cc and,healthy
R2572 T12931 T12923 punct ", ",used
R2573 T13077 T13075 conj fertile,healthy
R2574 T13078 T13059 punct ", ",were
R2575 T13079 T13080 mark although,observed
R2576 T12932 T12923 advcl encoding,used
R2577 T13080 T13059 advcl observed,were
R2578 T13081 T13082 nmod platelet,aggregation
R2579 T12933 T12934 det the,subunit
R2580 T13082 T13083 nmod aggregation,defects
R2581 T13083 T13080 nsubjpass defects,observed
R2582 T13084 T13082 cc and,aggregation
R2583 T13085 T13086 compound clot,retraction
R2584 T12934 T12932 dobj subunit,encoding
R2585 T13086 T13082 conj retraction,aggregation
R2586 T12935 T12934 amod small,subunit
R2587 T12936 T12934 amod regulatory,subunit
R2588 T12937 T12934 amod common,subunit
R2589 T13087 T13080 auxpass were,observed
R2590 T12938 T12937 prep to,common
R2591 T13088 T13089 punct [,33
R2592 T13089 T13059 parataxis 33,were
R2593 T13090 T13089 punct ],33
R2594 T12939 T12940 preconj both,calpain
R2595 T13091 T13051 punct .,narrowed
R2596 T12940 T12938 pobj calpain,to
R2597 T13093 T13094 prep At,left
R2598 T13095 T13096 det that,point
R2599 T12941 T12940 nmod μ,calpain
R2600 T13096 T13093 pobj point,At
R2601 T13097 T13094 punct ", ",left
R2602 T13098 T13094 nsubjpass we,left
R2603 T12942 T12941 punct -,μ
R2604 T13099 T13094 auxpass were,left
R2605 T13100 T13094 prep with,left
R2606 T13101 T13102 det the,possibilities
R2607 T12943 T12941 cc and,μ
R2608 T13102 T13100 pobj possibilities,with
R2609 T13103 T13102 amod last,possibilities
R2610 T13104 T13102 nummod two,possibilities
R2611 T12944 T12941 conj m,μ
R2612 T13105 T13106 mark that,required
R2613 T13106 T13102 advcl required,possibilities
R2614 T13107 T13108 det either,calpain
R2615 T13108 T13106 nsubjpass calpain,required
R2616 T13109 T13108 compound m,calpain
R2617 T13110 T13108 punct -,calpain
R2618 T13111 T13106 auxpass was,required
R2619 T13112 T13106 advmod specifically,required
R2620 T13113 T13106 prep during,required
R2621 T12945 T12940 punct -,calpain
R2622 T13114 T13113 pobj embryogenesis,during
R2623 T13115 T13106 punct ", ",required
R2624 T13116 T13106 cc or,required
R2625 T13117 T13118 mark that,was
R2626 T12946 T12923 punct .,used
R2627 T13118 T13106 conj was,required
R2628 T13119 T13120 det either,isoform
R2629 T13120 T13118 nsubj isoform,was
R2630 T13121 T13120 advmod alone,isoform
R2631 T13122 T13118 acomp sufficient,was
R2632 T12948 T12949 nummod Two,laboratories
R2633 T13123 T13122 prep for,sufficient
R2634 T13124 T13123 pcomp sustaining,for
R2635 T13125 T13126 amod embryonic,viability
R2636 T12949 T12951 nsubj laboratories,observed
R2637 T13126 T13124 dobj viability,sustaining
R2638 T13127 T13094 punct .,left
R2639 T13129 T13130 nsubj We,report
R2640 T12950 T12949 amod independent,laboratories
R2641 T13131 T13130 advmod here,report
R2642 T13132 T13133 mark that,die
R2643 T12952 T12953 amod embryonic,lethality
R2644 T13133 T13130 ccomp die,report
R2645 T13134 T13135 nmod Capn2,mice
R2646 T13135 T13133 nsubj mice,die
R2647 T13136 T13134 punct -,Capn2
R2648 T13137 T13134 punct /,Capn2
R2649 T13138 T13134 punct -,Capn2
R2650 T12953 T12951 dobj lethality,observed
R2651 T13139 T13135 punct ", ",mice
R2652 T13140 T13141 dep which,lack
R2653 T13141 T13135 relcl lack,mice
R2654 T12954 T12951 prep in,observed
R2655 T13142 T13143 det the,subunit
R2656 T13143 T13141 dobj subunit,lack
R2657 T13144 T13145 nmod m,calpain
R2658 T12955 T12956 compound Capn4,mice
R2659 T13145 T13143 nmod calpain,subunit
R2660 T13146 T13145 punct -,calpain
R2661 T13147 T13143 amod catalytic,subunit
R2662 T12956 T12954 pobj mice,in
R2663 T13148 T13133 punct ", ",die
R2664 T13149 T13133 prep at,die
R2665 T13150 T13151 det the,stage
R2666 T12957 T12956 compound knockout,mice
R2667 T13151 T13149 pobj stage,at
R2668 T13152 T13151 compound preimplantation,stage
R2669 T13153 T13151 prep of,stage
R2670 T13154 T13153 pobj development,of
R2671 T13155 T13130 punct .,report
R2672 T12958 T12951 punct ", ",observed
R2673 T13157 T13158 det This,observation
R2674 T12959 T12960 dep albeit,at
R2675 T12960 T12951 prep at,observed
R2676 T13158 T13159 nsubj observation,allows
R2677 T12961 T12962 amod different,stages
R2678 T13160 T13161 nsubj us,conclude
R2679 T13161 T13159 ccomp conclude,allows
R2680 T12962 T12960 pobj stages,at
R2681 T13162 T13161 aux to,conclude
R2682 T13163 T13161 advmod now,conclude
R2683 T13164 T13165 mark that,required
R2684 T12963 T12962 prep of,stages
R2685 T13165 T13161 ccomp required,conclude
R2686 T13166 T13167 compound m,calpain
R2687 T12964 T12963 pobj development,of
R2688 T13167 T13165 nsubjpass calpain,required
R2689 T13168 T13167 punct -,calpain
R2690 T13169 T13165 auxpass is,required
R2691 T12965 T12966 punct [,32
R2692 T13170 T13165 advmod specifically,required
R2693 T13171 T13165 prep during,required
R2694 T13172 T13171 pobj embryogenesis,during
R2695 T12966 T12951 parataxis 32,observed
R2696 T13173 T13159 punct .,allows
R2697 T12967 T12966 nummod 31,32
R2698 T13175 T13176 mark Since,expected
R2699 T13176 T13185 advcl expected,is
R2700 T13177 T13178 amod homozygous,disruption
R2701 T12968 T12966 punct ",",32
R2702 T13178 T13176 nsubjpass disruption,expected
R2703 T12969 T12966 punct ],32
R2704 T13179 T13178 prep of,disruption
R2705 T13180 T13181 det the,gene
R2706 T13181 T13179 pobj gene,of
R2707 T12970 T12951 punct .,observed
R2708 T13182 T13181 compound Capn4,gene
R2709 T13183 T13176 auxpass was,expected
R2710 T12972 T12973 det These,observations
R2711 T13184 T13176 advmod also,expected
R2712 T13186 T13187 aux to,abolish
R2713 T13187 T13176 xcomp abolish,expected
R2714 T12973 T12974 nsubj observations,supported
R2715 T13188 T13189 compound m,calpain
R2716 T13189 T13191 compound calpain,activity
R2717 T13190 T13189 punct -,calpain
R2718 T12974 T12975 ccomp supported,required
R2719 T13191 T13187 dobj activity,abolish
R2720 T13192 T13185 punct ", ",is
R2721 T12976 T12977 det the,hypothesis
R2722 T13193 T13194 det this,result
R2723 T13194 T13185 nsubj result,is
R2724 T12977 T12974 dobj hypothesis,supported
R2725 T13195 T13185 prep in,is
R2726 T12978 T12979 mark that,required
R2727 T13196 T13195 pobj agreement,in
R2728 T13197 T13196 prep with,agreement
R2729 T13198 T13199 det the,phenotype
R2730 T13199 T13197 pobj phenotype,with
R2731 T13200 T13199 acl presented,phenotype
R2732 T12979 T12977 acl required,hypothesis
R2733 T13201 T13200 agent by,presented
R2734 T13202 T13201 pobj one,by
R2735 T13203 T13202 prep of,one
R2736 T12980 T12981 det the,subunit
R2737 T13204 T13205 det the,lines
R2738 T13205 T13203 pobj lines,of
R2739 T13299 T13300 det the,cassette
R2740 T13206 T13205 nummod two,lines
R2741 T13207 T13208 npadvmod Capn4,targeted
R2742 T13208 T13205 amod targeted,lines
R2743 T13209 T13210 prep in,observed
R2744 T13300 T13298 pobj cassette,of
R2745 T13210 T13205 relcl observed,lines
R2746 T13211 T13209 pobj which,in
R2747 T13212 T13213 compound preimplantation,lethality
R2748 T13301 T13302 compound PGK,Neo
R2749 T13213 T13210 nsubjpass lethality,observed
R2750 T13214 T13213 prep of,lethality
R2751 T13215 T13216 amod null,embryos
R2752 T13216 T13214 pobj embryos,of
R2753 T13302 T13300 compound Neo,cassette
R2754 T13217 T13210 auxpass was,observed
R2755 T13218 T13210 advmod also,observed
R2756 T13219 T13220 punct [,32
R2757 T13303 T13302 punct -,Neo
R2758 T13304 T13297 prep into,insertion
R2759 T13220 T13210 parataxis 32,observed
R2760 T13305 T13306 det the,middle
R2761 T13221 T13220 punct ],32
R2762 T13222 T13185 punct .,is
R2763 T13306 T13304 pobj middle,into
R2764 T13224 T13225 det The,survival
R2765 T13307 T13306 prep of,middle
R2766 T13225 T13226 nsubj survival,is
R2767 T13308 T13307 pobj exon,of
R2768 T13227 T13225 prep of,survival
R2769 T13228 T13229 nmod Capn4,embryos
R2770 T13229 T13227 pobj embryos,of
R2771 T13230 T13228 punct -,Capn4
R2772 T13231 T13228 punct /,Capn4
R2773 T13232 T13228 punct -,Capn4
R2774 T13233 T13225 prep from,survival
R2775 T13309 T13308 nummod 9,exon
R2776 T13234 T13235 det the,line
R2777 T13235 T13233 pobj line,from
R2778 T13236 T13235 amod original,line
R2779 T13310 T13297 punct ", ",insertion
R2780 T13237 T13235 amod targeted,line
R2781 T13238 T13235 acl reported,line
R2782 T13311 T13312 dep which,caused
R2783 T13239 T13238 agent by,reported
R2784 T13240 T13239 pobj Arthur,by
R2785 T13241 T13240 cc and,Arthur
R2786 T13312 T13297 relcl caused,insertion
R2787 T13242 T13240 conj colleagues,Arthur
R2788 T13313 T13312 dobj truncation,caused
R2789 T13314 T13313 prep of,truncation
R2790 T13243 T13233 prep to,from
R2791 T13244 T13243 pobj mid-gestation,to
R2792 T13315 T13316 det the,protein
R2793 T13245 T13246 advmod more,difficult
R2794 T13246 T13226 acomp difficult,is
R2795 T13316 T13314 pobj protein,of
R2796 T13247 T13248 aux to,reconcile
R2797 T13248 T13246 advcl reconcile,difficult
R2798 T13249 T13250 punct [,31
R2799 T13317 T13318 punct [,31
R2800 T13250 T13226 parataxis 31,is
R2801 T13251 T13250 punct ],31
R2802 T13318 T13312 parataxis 31,caused
R2803 T13252 T13226 punct .,is
R2804 T13254 T13255 prep In,seems
R2805 T13319 T13318 punct ],31
R2806 T13256 T13254 pobj retrospect,In
R2807 T13320 T13280 punct .,involved
R2808 T13257 T13255 punct ", ",seems
R2809 T13258 T13255 nsubj it,seems
R2810 T13259 T13255 oprd likely,seems
R2811 T13322 T13323 det This,strategy
R2812 T13260 T13261 mark that,represents
R2813 T13261 T13255 ccomp represents,seems
R2814 T13262 T13263 det the,line
R2815 T13263 T13261 nsubj line,represents
R2816 T13323 T13324 nsubjpass strategy,based
R2817 T13264 T13263 amod latter,line
R2818 T13265 T13266 det a,state
R2819 T13325 T13324 auxpass was,based
R2820 T13266 T13261 dobj state,represents
R2821 T13267 T13266 amod hypomorphic,state
R2822 T13268 T13266 punct ", ",state
R2823 T13269 T13270 advmod rather,than
R2824 T13270 T13266 cc than,state
R2825 T13271 T13272 det a,null
R2826 T13272 T13266 conj null,state
R2827 T13273 T13272 amod true,null
R2828 T13274 T13255 punct .,seems
R2829 T13326 T13324 prep upon,based
R2830 T13276 T13277 det The,strategy
R2831 T13277 T13280 nsubj strategy,involved
R2832 T13327 T13328 amod previous,studies
R2833 T13278 T13277 compound Capn4,strategy
R2834 T13279 T13277 compound targeting,strategy
R2835 T13328 T13326 pobj studies,upon
R2836 T13281 T13277 acl employed,strategy
R2837 T13282 T13281 agent by,employed
R2838 T13283 T13282 pobj Arthur,by
R2839 T13284 T13283 cc and,Arthur
R2840 T13285 T13283 conj colleagues,Arthur
R2841 T13329 T13330 compound structure,function
R2842 T13286 T13280 xcomp disrupting,involved
R2843 T13287 T13288 det the,terminus
R2844 T13288 T13286 dobj terminus,disrupting
R2845 T13330 T13328 compound function,studies
R2846 T13289 T13288 compound C,terminus
R2847 T13290 T13288 punct -,terminus
R2848 T13291 T13288 prep of,terminus
R2849 T13292 T13293 det the,subunit
R2850 T13331 T13330 punct /,function
R2851 T13293 T13291 pobj subunit,of
R2852 T13294 T13293 nmod calpain,subunit
R2853 T13332 T13328 acl showing,studies
R2854 T13295 T13293 amod small,subunit
R2855 T13296 T13286 agent by,disrupting
R2856 T13297 T13296 pobj insertion,by
R2857 T13333 T13334 mark that,abolished
R2858 T13298 T13297 prep of,insertion
R2859 T13334 T13332 ccomp abolished,showing
R2860 T13335 T13334 nsubj excision,abolished
R2861 T13336 T13335 prep of,excision
R2862 T13405 T13406 det the,time
R2863 T13406 T13404 pobj time,in
R2864 T13337 T13338 det the,acid
R2865 T13407 T13406 prep of,time
R2866 T13408 T13407 pobj lethality,of
R2867 T13409 T13406 prep of,time
R2868 T13338 T13336 pobj acid,of
R2869 T13410 T13411 det these,lines
R2870 T13339 T13340 npadvmod C,terminal
R2871 T13340 T13338 amod terminal,acid
R2872 T13411 T13409 pobj lines,of
R2873 T13341 T13340 punct -,terminal
R2874 T13412 T13411 nummod two,lines
R2875 T13413 T13411 amod targeted,lines
R2876 T13414 T13411 compound Capn4,lines
R2877 T13415 T13401 aux can,explained
R2878 T13416 T13401 auxpass be,explained
R2879 T13417 T13401 agent by,explained
R2880 T13418 T13419 amod different,extents
R2881 T13342 T13338 nummod 25,acid
R2882 T13419 T13417 pobj extents,by
R2883 T13420 T13419 prep of,extents
R2884 T13421 T13420 pobj disruption,of
R2885 T13343 T13338 compound amino,acid
R2886 T13422 T13397 punct .,seems
R2887 T13344 T13338 nmod residues,acid
R2888 T13424 T13425 det The,allele
R2889 T13425 T13427 nsubj allele,represents
R2890 T13345 T13338 prep of,acid
R2891 T13426 T13425 compound Zimmerman,allele
R2892 T13346 T13347 det the,subunit
R2893 T13428 T13427 advmod probably,represents
R2894 T13429 T13430 det a,genotype
R2895 T13430 T13427 dobj genotype,represents
R2896 T13347 T13345 pobj subunit,of
R2897 T13431 T13430 amod true,genotype
R2898 T13432 T13430 amod null,genotype
R2899 T13433 T13434 mark while,is
R2900 T13348 T13347 amod small,subunit
R2901 T13434 T13427 advcl is,represents
R2902 T13435 T13436 det the,allele
R2903 T13436 T13434 nsubj allele,is
R2904 T13349 T13350 compound calpain,activity
R2905 T13437 T13436 compound Arthur,allele
R2906 T13438 T13434 advmod likely,is
R2907 T13439 T13440 det a,mutation
R2908 T13350 T13334 dobj activity,abolished
R2909 T13440 T13434 attr mutation,is
R2910 T13441 T13440 amod hypomorphic,mutation
R2911 T13442 T13427 punct .,represents
R2912 T13351 T13352 advmod when,co-expressed
R2913 T13444 T13445 amod Alternate,strategies
R2914 T13352 T13334 advcl co-expressed,abolished
R2915 T13445 T13447 nsubjpass strategies,shown
R2916 T13446 T13445 compound targeting,strategies
R2917 T13353 T13352 prep with,co-expressed
R2918 T13448 T13447 aux have,shown
R2919 T13449 T13447 auxpass been,shown
R2920 T13450 T13451 aux to,yield
R2921 T13354 T13355 det the,subunit
R2922 T13451 T13447 advcl yield,shown
R2923 T13452 T13453 amod different,phenotypes
R2924 T13453 T13451 dobj phenotypes,yield
R2925 T13355 T13353 pobj subunit,with
R2926 T13454 T13451 prep in,yield
R2927 T13455 T13456 compound gene,disruption
R2928 T13456 T13457 compound disruption,studies
R2929 T13457 T13454 pobj studies,in
R2930 T13458 T13447 punct .,shown
R2931 T13460 T13461 prep For,used
R2932 T13356 T13357 nmod m,calpain
R2933 T13462 T13460 pobj example,For
R2934 T13463 T13461 punct ", ",used
R2935 T13464 T13465 nummod three,strategies
R2936 T13465 T13461 nsubjpass strategies,used
R2937 T13466 T13465 amod different,strategies
R2938 T13357 T13355 nmod calpain,subunit
R2939 T13467 T13465 compound targeting,strategies
R2940 T13468 T13461 auxpass were,used
R2941 T13469 T13461 advmod independently,used
R2942 T13358 T13357 punct -,calpain
R2943 T13470 T13471 aux to,disrupt
R2944 T13471 T13461 advcl disrupt,used
R2945 T13359 T13355 amod large,subunit
R2946 T13472 T13473 det the,gene
R2947 T13473 T13471 dobj gene,disrupt
R2948 T13474 T13475 amod mixed,leukemia
R2949 T13475 T13473 nmod leukemia,gene
R2950 T13360 T13352 prep in,co-expressed
R2951 T13476 T13475 nmod lineage,leukemia
R2952 T13477 T13475 punct (,leukemia
R2953 T13361 T13362 compound E.,coli
R2954 T13478 T13475 appos Mll,leukemia
R2955 T13479 T13473 punct ),gene
R2956 T13480 T13461 punct .,used
R2957 T13362 T13360 pobj coli,in
R2958 T13482 T13483 prep In,perished
R2959 T13484 T13485 det all,studies
R2960 T13363 T13364 punct [,34
R2961 T13485 T13482 pobj studies,In
R2962 T13486 T13485 nummod three,studies
R2963 T13487 T13483 punct ", ",perished
R2964 T13364 T13334 parataxis 34,abolished
R2965 T13488 T13489 amod homozygous,null
R2966 T13489 T13490 amod null,embryos
R2967 T13490 T13483 nsubj embryos,perished
R2968 T13365 T13364 punct ],34
R2969 T13491 T13483 prep during,perished
R2970 T13492 T13491 pobj embryogenesis,during
R2971 T13366 T13324 punct .,based
R2972 T13493 T13491 punct ", ",during
R2973 T13494 T13491 cc but,during
R2974 T13495 T13491 conj at,during
R2975 T13496 T13497 amod different,stages
R2976 T13368 T13369 prep In,involved
R2977 T13497 T13495 pobj stages,at
R2978 T13498 T13499 punct (,E14.5
R2979 T13499 T13497 parataxis E14.5,stages
R2980 T13500 T13499 nmod E0.5,E14.5
R2981 T13501 T13499 punct ", ",E14.5
R2982 T13370 T13368 pobj contrast,In
R2983 T13502 T13499 nmod E10.5,E14.5
R2984 T13503 T13499 punct ", ",E14.5
R2985 T13504 T13499 punct ),E14.5
R2986 T13505 T13506 punct [,35
R2987 T13506 T13483 parataxis 35,perished
R2988 T13371 T13369 punct ", ",involved
R2989 T13507 T13506 punct ],35
R2990 T13508 T13483 punct .,perished
R2991 T13372 T13373 det the,strategy
R2992 T13510 T13511 det The,variation
R2993 T13373 T13369 nsubj strategy,involved
R2994 T13374 T13373 nmod Capn4,strategy
R2995 T13375 T13373 amod targeting,strategy
R2996 T13511 T13512 nsubjpass variation,attributed
R2997 T13376 T13373 acl employed,strategy
R2998 T13513 T13511 prep in,variation
R2999 T13377 T13376 agent by,employed
R3000 T13514 T13513 pobj phenotype,in
R3001 T13515 T13512 auxpass was,attributed
R3002 T13516 T13512 prep to,attributed
R3003 T13517 T13518 det the,differences
R3004 T13378 T13377 pobj Zimmerman,by
R3005 T13518 T13516 pobj differences,to
R3006 T13519 T13518 prep in,differences
R3007 T13520 T13519 pobj degree,in
R3008 T13379 T13378 cc and,Zimmerman
R3009 T13521 T13520 prep of,degree
R3010 T13522 T13521 pobj function,of
R3011 T13523 T13520 prep of,degree
R3012 T13380 T13378 conj colleagues,Zimmerman
R3013 T13524 T13525 det the,proteins
R3014 T13525 T13523 pobj proteins,of
R3015 T13526 T13525 amod truncated,proteins
R3016 T13381 T13382 det a,deletion
R3017 T13527 T13525 acl produced,proteins
R3018 T13528 T13527 prep from,produced
R3019 T13529 T13530 det the,alleles
R3020 T13382 T13369 dobj deletion,involved
R3021 T13530 T13528 pobj alleles,from
R3022 T13531 T13530 compound mutant,alleles
R3023 T13532 T13512 punct .,attributed
R3024 T13383 T13384 advmod much,more
R3025 T13534 T13535 det A,effect
R3026 T13535 T13537 nsubj effect,be
R3027 T13384 T13385 advmod more,extensive
R3028 T13536 T13535 amod similar,effect
R3029 T13538 T13537 aux might,be
R3030 T13385 T13382 amod extensive,deletion
R3031 T13539 T13537 prep at,be
R3032 T13540 T13539 pobj work,at
R3033 T13541 T13537 prep in,be
R3034 T13542 T13543 det the,lines
R3035 T13543 T13541 pobj lines,in
R3036 T13544 T13543 nummod two,lines
R3037 T13386 T13382 prep of,deletion
R3038 T13545 T13546 npadvmod Capn4,transgenic
R3039 T13546 T13543 amod transgenic,lines
R3040 T13547 T13537 punct .,be
R3041 T13387 T13388 nmod exons,4
R3042 T13549 T13550 nsubjpass Efforts,made
R3043 T13388 T13386 pobj 4,of
R3044 T13551 T13550 auxpass were,made
R3045 T13552 T13553 aux to,detect
R3046 T13553 T13550 advcl detect,made
R3047 T13389 T13388 prep through,4
R3048 T13554 T13553 dobj transcripts,detect
R3049 T13555 T13554 cc or,transcripts
R3050 T13390 T13389 pobj 8,through
R3051 T13556 T13557 compound calpain,activities
R3052 T13557 T13554 conj activities,transcripts
R3053 T13391 T13392 punct [,32
R3054 T13558 T13554 acl derived,transcripts
R3055 T13559 T13558 prep from,derived
R3056 T13560 T13561 det the,allele
R3057 T13561 T13559 pobj allele,from
R3058 T13392 T13369 parataxis 32,involved
R3059 T13562 T13563 compound Arthur,Capn4
R3060 T13563 T13561 compound Capn4,allele
R3061 T13564 T13550 punct .,made
R3062 T13393 T13392 punct ],32
R3063 T13566 T13567 det This,allele
R3064 T13567 T13568 nsubj allele,gave
R3065 T13394 T13369 punct .,involved
R3066 T13569 T13568 dobj rise,gave
R3067 T13396 T13397 nsubj It,seems
R3068 T13570 T13568 prep to,gave
R3069 T13571 T13572 amod multiple,species
R3070 T13572 T13570 pobj species,to
R3071 T13573 T13572 compound mRNA,species
R3072 T13398 T13397 advmod now,seems
R3073 T13574 T13572 punct ", ",species
R3074 T13575 T13572 amod detectable,species
R3075 T13576 T13575 prep by,detectable
R3076 T13577 T13578 compound RT,PCR
R3077 T13399 T13397 oprd probable,seems
R3078 T13578 T13576 pobj PCR,by
R3079 T13579 T13578 punct -,PCR
R3080 T13580 T13568 punct ", ",gave
R3081 T13400 T13401 mark that,explained
R3082 T13581 T13568 advcl reading,gave
R3083 T13582 T13581 advmod through,reading
R3084 T13583 T13581 prep from,reading
R3085 T13584 T13585 det the,half
R3086 T13585 T13583 pobj half,from
R3087 T13401 T13397 ccomp explained,seems
R3088 T13586 T13585 amod first,half
R3089 T13587 T13585 prep of,half
R3090 T13588 T13587 pobj exon,of
R3091 T13402 T13403 det the,difference
R3092 T13403 T13401 nsubjpass difference,explained
R3093 T13589 T13588 nummod 9,exon
R3094 T13590 T13583 prep to,from
R3095 T13404 T13403 prep in,difference
R3096 T13591 T13592 advmod at,two
R3097 T13592 T13594 nummod two,sites
R3098 T13593 T13592 advmod least,two
R3099 T13617 T13615 amod small,subunits
R3100 T13594 T13590 pobj sites,to
R3101 T13595 T13594 amod different,sites
R3102 T13596 T13594 amod cryptic,sites
R3103 T13597 T13594 compound splice,sites
R3104 T13618 T13610 prep with,give
R3105 T13598 T13594 prep in,sites
R3106 T13599 T13600 det the,sequence
R3107 T13619 T13620 quantmod 10,30
R3108 T13600 T13598 pobj sequence,in
R3109 T13601 T13600 compound PGK,sequence
R3110 T13602 T13600 compound promoter,sequence
R3111 T13620 T13622 nummod 30,acids
R3112 T13603 T13604 punct [,31
R3113 T13604 T13581 parataxis 31,reading
R3114 T13605 T13604 punct ],31
R3115 T13621 T13620 punct –,30
R3116 T13606 T13568 punct .,gave
R3117 T13608 T13609 det These,transcripts
R3118 T13622 T13618 pobj acids,with
R3119 T13609 T13610 nsubj transcripts,give
R3120 T13611 T13610 aux could,give
R3121 T13623 T13622 amod inappropriate,acids
R3122 T13612 T13610 dobj rise,give
R3123 T13613 T13610 prep to,give
R3124 T13614 T13615 amod defective,subunits
R3125 T13624 T13625 npadvmod C,terminal
R3126 T13615 T13613 pobj subunits,to
R3127 T13625 T13622 amod terminal,acids
R3128 T13626 T13625 punct -,terminal
R3129 T13616 T13615 nmod calpain,subunits
R3130 T13627 T13622 punct ", ",acids
R3131 T13628 T13629 dep which,be
R3132 T13629 T13622 relcl be,acids
R3133 T13723 T13721 pobj hands,in
R3134 T13630 T13629 aux might,be
R3135 T13724 T13725 advmod when,expressed
R3136 T13725 T13719 advcl expressed,observed
R3137 T13726 T13727 nmod calpain,subunits
R3138 T13727 T13725 nsubjpass subunits,expressed
R3139 T13728 T13727 amod large,subunits
R3140 T13729 T13725 auxpass were,expressed
R3141 T13631 T13629 acomp sufficient,be
R3142 T13730 T13725 advmod alone,expressed
R3143 T13731 T13732 preconj either,in
R3144 T13732 T13725 prep in,expressed
R3145 T13632 T13633 aux to,support
R3146 T13733 T13734 compound E.,coli
R3147 T13734 T13732 pobj coli,in
R3148 T13735 T13732 cc or,in
R3149 T13633 T13631 xcomp support,sufficient
R3150 T13736 T13732 conj in,in
R3151 T13737 T13738 amod mammalian,cells
R3152 T13634 T13635 det a,level
R3153 T13738 T13736 pobj cells,in
R3154 T13739 T13740 punct [,36
R3155 T13740 T13725 parataxis 36,expressed
R3156 T13635 T13633 dobj level,support
R3157 T13741 T13740 punct ],36
R3158 T13742 T13655 punct .,suggested
R3159 T13636 T13635 amod low,level
R3160 T13744 T13745 det The,timing
R3161 T13745 T13747 nsubj timing,be
R3162 T13637 T13635 prep of,level
R3163 T13746 T13745 amod different,timing
R3164 T13638 T13639 compound calpain,activity
R3165 T13748 T13745 prep of,timing
R3166 T13749 T13748 pobj lethality,of
R3167 T13750 T13745 prep in,timing
R3168 T13639 T13637 pobj activity,of
R3169 T13751 T13752 det the,knockouts
R3170 T13752 T13750 pobj knockouts,in
R3171 T13753 T13752 compound Capn4,knockouts
R3172 T13640 T13610 punct .,give
R3173 T13754 T13747 aux might,be
R3174 T13755 T13747 advmod also,be
R3175 T13756 T13757 det a,consequence
R3176 T13642 T13643 advmod However,give
R3177 T13643 T13655 ccomp give,suggested
R3178 T13757 T13747 attr consequence,be
R3179 T13758 T13757 prep of,consequence
R3180 T13644 T13643 punct ", ",give
R3181 T13759 T13760 det the,backgrounds
R3182 T13760 T13758 pobj backgrounds,of
R3183 T13761 T13760 amod different,backgrounds
R3184 T13645 T13643 nsubj expression,give
R3185 T13762 T13760 amod genetic,backgrounds
R3186 T13763 T13760 prep of,backgrounds
R3187 T13764 T13765 det the,lines
R3188 T13765 T13763 pobj lines,of
R3189 T13766 T13765 nummod two,lines
R3190 T13646 T13645 prep of,expression
R3191 T13767 T13765 amod transgenic,lines
R3192 T13768 T13757 punct ", ",consequence
R3193 T13769 T13770 dep which,observed
R3194 T13647 T13646 pobj calpains,of
R3195 T13770 T13757 relcl observed,consequence
R3196 T13771 T13770 aux has,observed
R3197 T13772 T13770 auxpass been,observed
R3198 T13648 T13647 prep with,calpains
R3199 T13773 T13774 aux to,influence
R3200 T13774 T13770 advcl influence,observed
R3201 T13649 T13650 det these,subunits
R3202 T13775 T13776 det the,phenotype
R3203 T13776 T13774 dobj phenotype,influence
R3204 T13777 T13776 prep of,phenotype
R3205 T13650 T13648 pobj subunits,with
R3206 T13778 T13779 amod transgenic,mice
R3207 T13779 T13777 pobj mice,of
R3208 T13651 T13650 amod modified,subunits
R3209 T13780 T13774 prep on,influence
R3210 T13781 T13782 det a,number
R3211 T13782 T13780 pobj number,on
R3212 T13652 T13650 amod small,subunits
R3213 T13783 T13782 prep of,number
R3214 T13784 T13783 pobj occasions,of
R3215 T13785 T13786 punct [,37
R3216 T13653 T13643 aux did,give
R3217 T13786 T13774 parataxis 37,influence
R3218 T13787 T13786 punct ],37
R3219 T13654 T13643 neg not,give
R3220 T13788 T13747 punct .,be
R3221 T13790 T13791 nsubj One,been
R3222 T13656 T13643 dobj rise,give
R3223 T13792 T13790 prep of,One
R3224 T13793 T13794 det the,questions
R3225 T13794 T13792 pobj questions,of
R3226 T13657 T13643 prep to,give
R3227 T13795 T13794 amod enduring,questions
R3228 T13796 T13794 prep in,questions
R3229 T13797 T13798 compound calpain,research
R3230 T13658 T13659 det any,activity
R3231 T13798 T13796 pobj research,in
R3232 T13799 T13791 aux has,been
R3233 T13800 T13801 mark whether,possess
R3234 T13659 T13657 pobj activity,to
R3235 T13801 T13791 ccomp possess,been
R3236 T13802 T13803 det the,isoforms
R3237 T13803 T13801 nsubj isoforms,possess
R3238 T13660 T13659 amod detectable,activity
R3239 T13804 T13803 nummod two,isoforms
R3240 T13805 T13803 amod ubiquitous,isoforms
R3241 T13806 T13803 punct ", ",isoforms
R3242 T13807 T13808 nmod μ,calpain
R3243 T13808 T13803 appos calpain,isoforms
R3244 T13661 T13659 compound calpain,activity
R3245 T13809 T13807 punct -,μ
R3246 T13810 T13807 cc and,μ
R3247 T13811 T13807 conj m,μ
R3248 T13812 T13808 punct -,calpain
R3249 T13662 T13663 advmod when,expressed
R3250 T13813 T13801 punct ", ",possess
R3251 T13814 T13815 amod distinct,roles
R3252 T13663 T13643 advcl expressed,give
R3253 T13815 T13801 dobj roles,possess
R3254 T13816 T13817 advmod in,vivo
R3255 T13817 T13815 amod vivo,roles
R3256 T13664 T13663 prep in,expressed
R3257 T13818 T13791 punct .,been
R3258 T13820 T13821 det The,enzymes
R3259 T13665 T13666 compound E.,coli
R3260 T13821 T13823 nsubj enzymes,share
R3261 T13822 T13821 nummod two,enzymes
R3262 T13666 T13664 pobj coli,in
R3263 T13824 T13825 nummod 62,%
R3264 T13825 T13826 compound %,identity
R3265 T13667 T13643 punct ", ",give
R3266 T13826 T13823 dobj identity,share
R3267 T13827 T13826 compound sequence,identity
R3268 T13828 T13823 cc and,share
R3269 T13668 T13669 mark although,has
R3270 T13669 T13643 advcl has,give
R3271 T13670 T13671 poss their,functionality
R3272 T13829 T13823 conj are,share
R3273 T13830 T13831 advmod very,similar
R3274 T13671 T13669 nsubj functionality,has
R3275 T13831 T13829 acomp similar,are
R3276 T13832 T13829 prep in,are
R3277 T13833 T13834 poss their,structure
R3278 T13672 T13671 prep in,functionality
R3279 T13834 T13832 pobj structure,in
R3280 T13835 T13834 cc and,structure
R3281 T13673 T13674 amod mammalian,cells
R3282 T13836 T13834 conj biochemistry,structure
R3283 T13837 T13823 punct .,share
R3284 T13674 T13672 pobj cells,in
R3285 T13839 T13840 advmod Notably,cleave
R3286 T13675 T13669 advmod yet,has
R3287 T13841 T13840 punct ", ",cleave
R3288 T13842 T13840 nsubj they,cleave
R3289 T13843 T13840 advmod essentially,cleave
R3290 T13676 T13677 aux to,determined
R3291 T13677 T13669 xcomp determined,has
R3292 T13844 T13845 det the,set
R3293 T13845 T13840 dobj set,cleave
R3294 T13678 T13677 auxpass be,determined
R3295 T13846 T13845 amod same,set
R3296 T13847 T13845 prep of,set
R3297 T13848 T13847 pobj substrates,of
R3298 T13679 T13680 punct (,J.S.
R3299 T13849 T13850 advmod in,vitro
R3300 T13850 T13840 advmod vitro,cleave
R3301 T13680 T13669 meta J.S.,has
R3302 T13851 T13840 punct ", ",cleave
R3303 T13852 T13840 advcl suggesting,cleave
R3304 T13681 T13680 nmod Elce,J.S.
R3305 T13853 T13854 mark that,have
R3306 T13854 T13852 ccomp have,suggesting
R3307 T13855 T13854 nsubj they,have
R3308 T13682 T13680 punct ", ",J.S.
R3309 T13856 T13857 det the,potential
R3310 T13857 T13854 dobj potential,have
R3311 T13683 T13680 amod unpublished,J.S.
R3312 T13858 T13859 aux to,carry
R3313 T13859 T13857 acl carry,potential
R3314 T13860 T13859 prt out,carry
R3315 T13684 T13680 nmod work,J.S.
R3316 T13861 T13862 det the,functions
R3317 T13862 T13859 dobj functions,carry
R3318 T13863 T13862 amod same,functions
R3319 T13685 T13680 punct ),J.S.
R3320 T13864 T13865 advmod in,vivo
R3321 T13865 T13859 advmod vivo,carry
R3322 T13686 T13655 nsubjpass It,suggested
R3323 T13866 T13840 punct .,cleave
R3324 T13687 T13655 aux has,suggested
R3325 T13868 T13869 prep On,is
R3326 T13688 T13655 advmod also,suggested
R3327 T13870 T13871 det the,hand
R3328 T13871 T13868 pobj hand,On
R3329 T13872 T13871 amod other,hand
R3330 T13689 T13655 auxpass been,suggested
R3331 T13873 T13869 punct ", ",is
R3332 T13874 T13875 mark since,require
R3333 T13875 T13869 advcl require,is
R3334 T13690 T13691 mark that,provide
R3335 T13876 T13875 nsubj they,require
R3336 T13877 T13878 amod different,amounts
R3337 T13878 T13875 dobj amounts,require
R3338 T13691 T13655 ccomp provide,suggested
R3339 T13879 T13878 prep of,amounts
R3340 T13880 T13879 pobj Ca2,of
R3341 T13881 T13880 punct +,Ca2
R3342 T13692 T13693 nmod calpain,subunits
R3343 T13882 T13875 prep for,require
R3344 T13883 T13884 nmod in,vitro
R3345 T13884 T13885 nmod vitro,activation
R3346 T13885 T13882 pobj activation,for
R3347 T13886 T13869 punct ", ",is
R3348 T13693 T13691 nsubj subunits,provide
R3349 T13887 T13869 nsubj it,is
R3350 T13888 T13869 acomp possible,is
R3351 T13889 T13890 mark that,regulated
R3352 T13694 T13693 amod large,subunits
R3353 T13890 T13869 ccomp regulated,is
R3354 T13891 T13892 det the,isoforms
R3355 T13695 T13693 advmod alone,subunits
R3356 T13892 T13890 nsubjpass isoforms,regulated
R3357 T13893 T13892 nummod two,isoforms
R3358 T13696 T13691 aux might,provide
R3359 T13894 T13890 auxpass are,regulated
R3360 T13895 T13890 advmod differentially,regulated
R3361 T13896 T13890 prep inside,regulated
R3362 T13697 T13698 det some,activity
R3363 T13897 T13896 pobj cells,inside
R3364 T13898 T13869 punct .,is
R3365 T13698 T13691 dobj activity,provide
R3366 T13900 T13901 nsubj It,is
R3367 T13699 T13691 prep in,provide
R3368 T13902 T13901 advmod now,is
R3369 T13903 T13901 acomp clear,is
R3370 T13904 T13901 punct ", ",is
R3371 T13700 T13701 amod eukaryotic,cells
R3372 T13905 T13901 prep from,is
R3373 T13906 T13907 det the,work
R3374 T13907 T13905 pobj work,from
R3375 T13701 T13699 pobj cells,in
R3376 T13908 T13909 compound gene,targeting
R3377 T13909 T13907 compound targeting,work
R3378 T13910 T13907 acl done,work
R3379 T13702 T13691 punct ", ",provide
R3380 T13911 T13910 prep in,done
R3381 T13912 T13911 pobj mice,in
R3382 T13703 T13704 mark although,appears
R3383 T13913 T13901 punct ", ",is
R3384 T13914 T13915 mark that,have
R3385 T13915 T13901 ccomp have,is
R3386 T13704 T13691 advcl appears,provide
R3387 T13916 T13917 nmod μ,calpain
R3388 T13917 T13915 nsubj calpain,have
R3389 T13918 T13916 punct -,μ
R3390 T13705 T13706 det the,knockout
R3391 T13919 T13916 cc and,μ
R3392 T13920 T13916 conj m,μ
R3393 T13921 T13917 punct -,calpain
R3394 T13706 T13704 nsubj knockout,appears
R3395 T13922 T13923 det some,roles
R3396 T13923 T13915 dobj roles,have
R3397 T13924 T13923 amod distinct,roles
R3398 T13925 T13923 amod physiological,roles
R3399 T13926 T13915 punct ", ",have
R3400 T13707 T13706 nmod Zimmerman,knockout
R3401 T13927 T13928 advmod at,least
R3402 T13928 T13929 advmod least,during
R3403 T13708 T13709 advmod et,al.
R3404 T13929 T13915 prep during,have
R3405 T13709 T13707 advmod al.,Zimmerman
R3406 T13930 T13929 pobj embryogenesis,during
R3407 T13931 T13901 punct .,is
R3408 T13710 T13711 aux to,exclude
R3409 T13933 T13934 mark As,noted
R3410 T13711 T13704 xcomp exclude,appears
R3411 T13934 T13935 advcl noted,affect
R3412 T13712 T13713 det that,possibility
R3413 T13713 T13711 dobj possibility,exclude
R3414 T13936 T13935 punct ", ",affect
R3415 T13714 T13691 punct ", ",provide
R3416 T13937 T13938 mark whereas,die
R3417 T13938 T13935 advcl die,affect
R3418 T13715 T13691 cc and,provide
R3419 T13939 T13940 npadvmod Capn2,null
R3420 T13940 T13941 amod null,embryos
R3421 T13941 T13938 nsubj embryos,die
R3422 T13716 T13717 det no,activity
R3423 T13942 T13941 amod murine,embryos
R3424 T13943 T13944 amod prior,to
R3425 T13717 T13719 nsubjpass activity,observed
R3426 T13944 T13938 prep to,die
R3427 T13945 T13944 pobj implantation,to
R3428 T13946 T13935 punct ", ",affect
R3429 T13718 T13717 compound calpain,activity
R3430 T13947 T13948 amod homozygous,disruption
R3431 T13948 T13935 nsubj disruption,affect
R3432 T13949 T13948 prep of,disruption
R3433 T13719 T13691 conj observed,provide
R3434 T13950 T13951 det the,gene
R3435 T13951 T13949 pobj gene,of
R3436 T13720 T13719 auxpass was,observed
R3437 T13952 T13953 nmod μ,calpain
R3438 T13953 T13951 nmod calpain,gene
R3439 T13954 T13953 punct -,calpain
R3440 T13721 T13719 prep in,observed
R3441 T13955 T13951 amod large,gene
R3442 T13956 T13951 compound subunit,gene
R3443 T13722 T13723 poss our,hands
R3444 T13957 T13951 punct ", ",gene
R3445 T13958 T13951 appos Capn1,gene
R3446 T13959 T13935 punct ", ",affect
R3447 T13960 T13935 aux did,affect
R3448 T13961 T13935 neg not,affect
R3449 T13962 T13963 det the,viability
R3450 T14041 T14040 amod relative,amounts
R3451 T13963 T13935 dobj viability,affect
R3452 T13964 T13963 prep of,viability
R3453 T13965 T13964 pobj mice,of
R3454 T13966 T13967 punct [,33
R3455 T13967 T13935 parataxis 33,affect
R3456 T13968 T13967 punct ],33
R3457 T14042 T14013 punct .,are
R3458 T13969 T13935 punct .,affect
R3459 T13971 T13972 det The,phenotype
R3460 T14044 T14045 nsubj Platelets,contain
R3461 T13972 T13974 nsubj phenotype,involved
R3462 T13973 T13972 amod principal,phenotype
R3463 T14046 T14044 cc and,Platelets
R3464 T13975 T13972 acl observed,phenotype
R3465 T13976 T13975 prep as,observed
R3466 T13977 T13978 det a,result
R3467 T13978 T13976 pobj result,as
R3468 T14047 T14044 conj erythrocytes,Platelets
R3469 T13979 T13978 prep of,result
R3470 T13980 T13981 compound Capn1,deficiency
R3471 T13981 T13979 pobj deficiency,of
R3472 T14048 T14049 amod abundant,activity
R3473 T13982 T13983 det a,disturbance
R3474 T13983 T13974 dobj disturbance,involved
R3475 T13984 T13983 prep in,disturbance
R3476 T14049 T14045 dobj activity,contain
R3477 T13985 T13986 compound platelet,function
R3478 T13986 T13984 pobj function,in
R3479 T13987 T13983 punct ", ",disturbance
R3480 T14050 T14051 compound μ,calpain
R3481 T13988 T13989 advmod possibly,at
R3482 T13989 T13983 prep at,disturbance
R3483 T13990 T13991 det the,level
R3484 T14051 T14049 compound calpain,activity
R3485 T13991 T13989 pobj level,at
R3486 T13992 T13991 prep of,level
R3487 T13993 T13994 det the,phosphorylation
R3488 T14052 T14051 punct -,calpain
R3489 T13994 T13992 pobj phosphorylation,of
R3490 T13995 T13994 compound tyrosine,phosphorylation
R3491 T13996 T13994 prep of,phosphorylation
R3492 T14053 T14045 punct ", ",contain
R3493 T13997 T13998 amod certain,proteins
R3494 T13998 T13996 pobj proteins,of
R3495 T13999 T13998 acl involved,proteins
R3496 T14054 T14055 mark while,is
R3497 T14055 T14045 advcl is,contain
R3498 T14000 T13999 prep in,involved
R3499 T14001 T14002 compound platelet,activation
R3500 T14002 T14000 pobj activation,in
R3501 T14003 T13974 punct .,involved
R3502 T14056 T14057 compound m,calpain
R3503 T14005 T14006 preconj Both,activities
R3504 T14006 T14013 nsubj activities,are
R3505 T14057 T14059 compound calpain,activity
R3506 T14007 T14008 nmod μ,calpain
R3507 T14008 T14006 compound calpain,activities
R3508 T14009 T14007 punct -,μ
R3509 T14058 T14057 punct -,calpain
R3510 T14010 T14007 cc and,μ
R3511 T14011 T14007 conj m,μ
R3512 T14012 T14008 punct -,calpain
R3513 T14059 T14055 nsubj activity,is
R3514 T14014 T14013 acomp present,are
R3515 T14060 T14061 advmod barely,detectable
R3516 T14015 T14013 prep in,are
R3517 T14061 T14055 acomp detectable,is
R3518 T14062 T14045 punct .,contain
R3519 T14016 T14017 amod most,cells
R3520 T14017 T14015 pobj cells,in
R3521 T14018 T14017 amod mammalian,cells
R3522 T14064 T14065 nsubj Compensation,is
R3523 T14019 T14013 punct ", ",are
R3524 T14020 T14021 mark although,provide
R3525 T14066 T14064 prep for,Compensation
R3526 T14021 T14013 advcl provide,are
R3527 T14022 T14023 det the,data
R3528 T14023 T14021 nsubj data,provide
R3529 T14024 T14023 amod published,data
R3530 T14067 T14068 compound μ,calpain
R3531 T14025 T14023 punct ", ",data
R3532 T14026 T14023 prep owing,data
R3533 T14027 T14026 prep to,owing
R3534 T14068 T14070 compound calpain,deficiency
R3535 T14028 T14027 pobj weaknesses,to
R3536 T14029 T14028 prep in,weaknesses
R3537 T14030 T14031 det the,methodology
R3538 T14069 T14068 punct -,calpain
R3539 T14031 T14029 pobj methodology,in
R3540 T14032 T14031 amod available,methodology
R3541 T14033 T14021 punct ", ",provide
R3542 T14070 T14066 pobj deficiency,for
R3543 T14034 T14021 aux do,provide
R3544 T14035 T14021 neg not,provide
R3545 T14036 T14037 amod reliable,estimates
R3546 T14071 T14064 prep by,Compensation
R3547 T14037 T14021 dobj estimates,provide
R3548 T14038 T14037 prep of,estimates
R3549 T14039 T14040 poss their,amounts
R3550 T14072 T14073 compound m,calpain
R3551 T14040 T14038 pobj amounts,of
R3552 T14073 T14071 pobj calpain,by
R3553 T14074 T14073 punct -,calpain
R3554 T14075 T14065 advmod therefore,is
R3555 T14147 T14145 punct -,Capn2
R3556 T14076 T14077 advmod less,likely
R3557 T14148 T14145 punct /,Capn2
R3558 T14149 T14145 punct -,Capn2
R3559 T14077 T14065 acomp likely,is
R3560 T14150 T14151 mark that,is
R3561 T14151 T14140 ccomp is,demonstrates
R3562 T14078 T14077 prep in,likely
R3563 T14152 T14153 compound m,calpain
R3564 T14153 T14155 compound calpain,activity
R3565 T14154 T14153 punct -,calpain
R3566 T14079 T14078 pobj platelets,in
R3567 T14155 T14151 nsubj activity,is
R3568 T14156 T14151 acomp essential,is
R3569 T14157 T14156 prep for,essential
R3570 T14080 T14079 cc and,platelets
R3571 T14158 T14159 amod embryonic,development
R3572 T14159 T14157 pobj development,for
R3573 T14160 T14159 prep beyond,development
R3574 T14081 T14079 conj erythrocytes,platelets
R3575 T14161 T14162 det the,stage
R3576 T14162 T14160 pobj stage,beyond
R3577 T14082 T14077 prep than,likely
R3578 T14163 T14164 nummod 8,cell
R3579 T14164 T14162 compound cell,stage
R3580 T14165 T14164 punct -,cell
R3581 T14083 T14082 prep in,than
R3582 T14166 T14140 punct .,demonstrates
R3583 T14168 T14169 nsubj It,follows
R3584 T14084 T14085 amod other,types
R3585 T14170 T14171 mark that,are
R3586 T14171 T14169 ccomp are,follows
R3587 T14085 T14083 pobj types,in
R3588 T14172 T14173 advmod at,least
R3589 T14173 T14174 advmod least,functions
R3590 T14086 T14085 compound cell,types
R3591 T14174 T14171 nsubj functions,are
R3592 T14175 T14174 det some,functions
R3593 T14176 T14174 prep of,functions
R3594 T14087 T14065 punct .,is
R3595 T14177 T14178 compound μ,calpain
R3596 T14178 T14176 pobj calpain,of
R3597 T14179 T14178 punct -,calpain
R3598 T14089 T14090 prep As,is
R3599 T14180 T14178 cc and,calpain
R3600 T14181 T14182 compound m,calpain
R3601 T14182 T14178 conj calpain,calpain
R3602 T14183 T14182 punct -,calpain
R3603 T14184 T14171 acomp distinct,are
R3604 T14185 T14169 punct .,follows
R3605 T14091 T14092 det a,result
R3606 T14187 T14188 det The,cause
R3607 T14092 T14089 pobj result,As
R3608 T14188 T14190 nsubjpass cause,clarified
R3609 T14189 T14188 amod underlying,cause
R3610 T14093 T14090 punct ", ",is
R3611 T14191 T14188 prep of,cause
R3612 T14192 T14193 det the,lethality
R3613 T14193 T14191 pobj lethality,of
R3614 T14094 T14090 nsubj it,is
R3615 T14194 T14193 compound preimplantation,lethality
R3616 T14195 T14188 prep in,cause
R3617 T14196 T14197 nmod Capn2,embryos
R3618 T14095 T14090 neg not,is
R3619 T14197 T14195 pobj embryos,in
R3620 T14198 T14196 punct -,Capn2
R3621 T14096 T14090 acomp possible,is
R3622 T14199 T14196 punct /,Capn2
R3623 T14200 T14196 punct -,Capn2
R3624 T14201 T14190 aux has,clarified
R3625 T14097 T14098 aux to,determine
R3626 T14202 T14190 neg not,clarified
R3627 T14203 T14190 advmod yet,clarified
R3628 T14204 T14190 auxpass been,clarified
R3629 T14098 T14090 xcomp determine,is
R3630 T14205 T14190 punct .,clarified
R3631 T14099 T14100 mark whether,is
R3632 T14207 T14208 det The,embryos
R3633 T14208 T14212 nsubj embryos,present
R3634 T14209 T14208 nummod two,embryos
R3635 T14100 T14098 advcl is,determine
R3636 T14210 T14211 amod homozygous,null
R3637 T14211 T14208 amod null,embryos
R3638 T14101 T14102 det the,absence
R3639 T14213 T14208 acl identified,embryos
R3640 T14214 T14213 prep at,identified
R3641 T14215 T14216 det the,stage
R3642 T14102 T14100 nsubj absence,is
R3643 T14216 T14214 pobj stage,at
R3644 T14217 T14218 nummod 8,cell
R3645 T14218 T14216 compound cell,stage
R3646 T14103 T14102 prep of,absence
R3647 T14219 T14218 punct -,cell
R3648 T14220 T14212 aux did,present
R3649 T14221 T14212 neg not,present
R3650 T14104 T14105 amod marked,phenotype
R3651 T14222 T14223 det any,defects
R3652 T14223 T14212 dobj defects,present
R3653 T14105 T14103 pobj phenotype,of
R3654 T14224 T14223 amod obvious,defects
R3655 T14225 T14223 amod morphological,defects
R3656 T14226 T14212 punct .,present
R3657 T14228 T14229 advmod However,is
R3658 T14106 T14102 prep in,absence
R3659 T14230 T14229 punct ", ",is
R3660 T14231 T14232 det the,fact
R3661 T14232 T14229 nsubj fact,is
R3662 T14107 T14108 nmod Capn1,animals
R3663 T14233 T14234 mark that,proved
R3664 T14234 T14232 acl proved,fact
R3665 T14235 T14236 advmod only,embryos
R3666 T14108 T14106 pobj animals,in
R3667 T14236 T14234 nsubj embryos,proved
R3668 T14237 T14238 quantmod two,90
R3669 T14238 T14236 nummod 90,embryos
R3670 T14109 T14107 punct -,Capn1
R3671 T14239 T14238 quantmod out,90
R3672 T14240 T14238 quantmod of,90
R3673 T14241 T14242 advmod successfully,genotyped
R3674 T14110 T14107 punct /,Capn1
R3675 T14242 T14236 amod genotyped,embryos
R3676 T14243 T14236 amod pre-implantation,embryos
R3677 T14244 T14245 aux to,be
R3678 T14111 T14107 punct -,Capn1
R3679 T14245 T14234 xcomp be,proved
R3680 T14246 T14245 attr Capn2,be
R3681 T14247 T14246 punct -,Capn2
R3682 T14112 T14100 prep due,is
R3683 T14248 T14246 punct /,Capn2
R3684 T14249 T14246 punct -,Capn2
R3685 T14113 T14112 pcomp to,due
R3686 T14250 T14229 prep in,is
R3687 T14251 T14250 pobj itself,in
R3688 T14252 T14229 acomp revealing,is
R3689 T14114 T14115 det a,affect
R3690 T14115 T14112 pobj affect,due
R3691 T14116 T14115 amod compensatory,affect
R3692 T14253 T14229 punct .,is
R3693 T14117 T14115 prep by,affect
R3694 T14255 T14256 compound Preimplantation,lethality
R3695 T14256 T14257 nsubjpass lethality,attributed
R3696 T14118 T14119 det the,activity
R3697 T14258 T14256 acl resulting,lethality
R3698 T14259 T14258 prep from,resulting
R3699 T14119 T14117 pobj activity,by
R3700 T14260 T14261 compound gene,knockouts
R3701 T14261 T14259 pobj knockouts,from
R3702 T14120 T14119 amod remaining,activity
R3703 T14262 T14257 aux can,attributed
R3704 T14263 T14257 advmod typically,attributed
R3705 T14264 T14257 auxpass be,attributed
R3706 T14265 T14257 prep to,attributed
R3707 T14266 T14267 nummod two,causes
R3708 T14267 T14265 pobj causes,to
R3709 T14121 T14122 compound m,calpain
R3710 T14268 T14267 amod general,causes
R3711 T14269 T14257 punct .,attributed
R3712 T14122 T14119 compound calpain,activity
R3713 T14271 T14272 prep In,incurred
R3714 T14273 T14274 det some,cases
R3715 T14123 T14122 punct -,calpain
R3716 T14274 T14271 pobj cases,In
R3717 T14275 T14272 punct ", ",incurred
R3718 T14124 T14100 punct ", ",is
R3719 T14276 T14272 nsubjpass defects,incurred
R3720 T14277 T14272 auxpass are,incurred
R3721 T14278 T14272 prep in,incurred
R3722 T14125 T14100 cc or,is
R3723 T14126 T14127 mark whether,are
R3724 T14279 T14280 det the,program
R3725 T14127 T14100 conj are,is
R3726 T14280 T14278 pobj program,in
R3727 T14281 T14280 amod embryonic,program
R3728 T14282 T14280 compound differentiation,program
R3729 T14128 T14129 det the,functions
R3730 T14283 T14284 dep which,observed
R3731 T14284 T14280 relcl observed,program
R3732 T14285 T14284 aux can,observed
R3733 T14129 T14127 nsubj functions,are
R3734 T14286 T14284 advmod often,observed
R3735 T14287 T14284 auxpass be,observed
R3736 T14130 T14129 prep of,functions
R3737 T14288 T14284 advmod morphologically,observed
R3738 T14289 T14290 punct [,38
R3739 T14131 T14132 compound μ,calpain
R3740 T14290 T14284 parataxis 38,observed
R3741 T14291 T14290 punct ],38
R3742 T14292 T14272 punct .,incurred
R3743 T14132 T14130 pobj calpain,of
R3744 T14294 T14295 amod Null,embryos
R3745 T14295 T14296 nsubj embryos,survive
R3746 T14133 T14132 punct -,calpain
R3747 T14297 T14295 prep of,embryos
R3748 T14298 T14299 det this,type
R3749 T14134 T14127 advmod simply,are
R3750 T14299 T14297 pobj type,of
R3751 T14300 T14296 advmod often,survive
R3752 T14135 T14127 neg not,are
R3753 T14301 T14296 prep beyond,survive
R3754 T14302 T14303 det the,stage
R3755 T14303 T14301 pobj stage,beyond
R3756 T14136 T14127 acomp essential,are
R3757 T14304 T14303 compound morula,stage
R3758 T14305 T14296 punct ", ",survive
R3759 T14306 T14296 cc and,survive
R3760 T14307 T14308 det a,distribution
R3761 T14308 T14310 nsubjpass distribution,noted
R3762 T14137 T14090 punct .,is
R3763 T14309 T14308 amod Mendelian,distribution
R3764 T14310 T14296 conj noted,survive
R3765 T14311 T14308 prep of,distribution
R3766 T14139 T14140 prep In,demonstrates
R3767 T14312 T14313 amod embryonic,genotypes
R3768 T14313 T14311 pobj genotypes,of
R3769 T14314 T14310 auxpass is,noted
R3770 T14315 T14310 advmod usually,noted
R3771 T14141 T14139 pobj contrast,In
R3772 T14316 T14310 punct .,noted
R3773 T14318 T14319 prep In,thought
R3774 T14142 T14140 punct ", ",demonstrates
R3775 T14320 T14321 amod other,cases
R3776 T14321 T14318 pobj cases,In
R3777 T14322 T14319 punct ", ",thought
R3778 T14143 T14140 nsubj lethality,demonstrates
R3779 T14323 T14319 advmod however,thought
R3780 T14324 T14319 punct ", ",thought
R3781 T14144 T14143 prep in,lethality
R3782 T14325 T14326 det the,disruption
R3783 T14326 T14319 nsubjpass disruption,thought
R3784 T14327 T14326 compound gene,disruption
R3785 T14145 T14146 nmod Capn2,embryos
R3786 T14328 T14319 auxpass is,thought
R3787 T14329 T14330 aux to,compromise
R3788 T14146 T14144 pobj embryos,in
R3789 T14330 T14319 xcomp compromise,thought
R3790 T14331 T14330 advmod fatally,compromise
R3791 T14332 T14333 det the,viability
R3792 T14359 T14350 auxpass are,observed
R3793 T14333 T14330 dobj viability,compromise
R3794 T14334 T14333 prep of,viability
R3795 T14335 T14334 pobj cells,of
R3796 T14336 T14335 prep in,cells
R3797 T14360 T14350 advmod ever,observed
R3798 T14337 T14338 det the,embryo
R3799 T14338 T14336 pobj embryo,in
R3800 T14339 T14338 amod early,embryo
R3801 T14361 T14350 cc and,observed
R3802 T14340 T14341 punct [,39
R3803 T14341 T14330 parataxis 39,compromise
R3804 T14342 T14341 dep 3,39
R3805 T14362 T14363 amod homozygous,cells
R3806 T14343 T14341 punct ",",39
R3807 T14344 T14345 punct -,43
R3808 T14345 T14341 prep 43,39
R3809 T14363 T14366 nsubjpass cells,isolated
R3810 T14346 T14341 punct ],39
R3811 T14347 T14319 punct .,thought
R3812 T14349 T14350 prep In,observed
R3813 T14364 T14363 compound mutant,cells
R3814 T14351 T14352 det these,instances
R3815 T14352 T14349 pobj instances,In
R3816 T14353 T14350 punct ", ",observed
R3817 T14365 T14363 compound ES,cells
R3818 T14354 T14355 advmod only,embryos
R3819 T14355 T14350 nsubjpass embryos,observed
R3820 T14356 T14355 det a,embryos
R3821 T14366 T14350 conj isolated,observed
R3822 T14357 T14355 amod few,embryos
R3823 T14358 T14355 amod null,embryos
R3824 T14367 T14366 aux could,isolated
R3825 T14368 T14366 neg not,isolated
R3826 T14369 T14366 auxpass be,isolated
R3827 T14370 T14366 punct .,isolated
R3828 T14372 T14373 det The,knockout
R3829 T14465 T14467 compound calpain,involvement
R3830 T14373 T14375 nsubj knockout,fits
R3831 T14466 T14465 punct -,calpain
R3832 T14467 T14463 pobj involvement,of
R3833 T14468 T14467 prep in,involvement
R3834 T14374 T14373 compound Capn2,knockout
R3835 T14469 T14470 compound cell,proliferation
R3836 T14470 T14468 pobj proliferation,in
R3837 T14376 T14375 prep into,fits
R3838 T14471 T14467 prep in,involvement
R3839 T14472 T14473 amod certain,circumstances
R3840 T14473 T14471 pobj circumstances,in
R3841 T14474 T14462 punct ", ",reports
R3842 T14377 T14378 det the,category
R3843 T14475 T14462 prep including,reports
R3844 T14476 T14475 pobj reports,including
R3845 T14477 T14476 prep of,reports
R3846 T14378 T14376 pobj category,into
R3847 T14478 T14479 poss its,involvement
R3848 T14479 T14477 pobj involvement,of
R3849 T14480 T14479 prep in,involvement
R3850 T14379 T14378 amod latter,category
R3851 T14481 T14482 compound chromosome,segregation
R3852 T14482 T14480 pobj segregation,in
R3853 T14380 T14375 punct .,fits
R3854 T14483 T14482 prep during,segregation
R3855 T14484 T14483 pobj mitosis,during
R3856 T14485 T14486 punct [,44
R3857 T14382 T14383 mark If,is
R3858 T14486 T14484 parataxis 44,mitosis
R3859 T14487 T14486 punct ],44
R3860 T14488 T14489 advmod as,as
R3861 T14489 T14483 cc as,during
R3862 T14490 T14489 advmod well,as
R3863 T14491 T14483 conj during,during
R3864 T14492 T14491 pobj meiosis,during
R3865 T14383 T14385 advcl is,is
R3866 T14493 T14494 punct [,45
R3867 T14494 T14492 parataxis 45,meiosis
R3868 T14495 T14494 punct ],45
R3869 T14384 T14383 nsubj it,is
R3870 T14496 T14459 punct .,been
R3871 T14386 T14383 acomp true,is
R3872 T14498 T14499 nsubj Defects,contribute
R3873 T14500 T14498 prep in,Defects
R3874 T14501 T14500 pobj migration,in
R3875 T14387 T14388 mark that,is
R3876 T14502 T14498 punct ", ",Defects
R3877 T14503 T14498 acl reported,Defects
R3878 T14504 T14503 prep in,reported
R3879 T14388 T14383 ccomp is,is
R3880 T14505 T14506 nmod Capn4,cells
R3881 T14506 T14504 pobj cells,in
R3882 T14507 T14505 punct -,Capn4
R3883 T14389 T14390 compound m,calpain
R3884 T14508 T14505 punct /,Capn4
R3885 T14509 T14505 punct -,Capn4
R3886 T14390 T14388 nsubj calpain,is
R3887 T14510 T14511 punct [,9
R3888 T14511 T14503 parataxis 9,reported
R3889 T14512 T14511 punct ],9
R3890 T14391 T14390 punct -,calpain
R3891 T14513 T14499 punct ", ",contribute
R3892 T14514 T14499 aux could,contribute
R3893 T14515 T14499 prep to,contribute
R3894 T14392 T14388 acomp essential,is
R3895 T14516 T14517 amod failed,development
R3896 T14393 T14392 prep for,essential
R3897 T14517 T14515 pobj development,to
R3898 T14518 T14517 amod embryonic,development
R3899 T14519 T14499 punct .,contribute
R3900 T14394 T14395 det some,aspect
R3901 T14521 T14522 det The,association
R3902 T14522 T14523 nsubjpass association,noted
R3903 T14395 T14393 pobj aspect,for
R3904 T14524 T14522 prep between,association
R3905 T14396 T14395 prep of,aspect
R3906 T14525 T14524 pobj calpain,between
R3907 T14526 T14525 cc and,calpain
R3908 T14397 T14398 compound cell,viability
R3909 T14527 T14528 compound cell,viability
R3910 T14528 T14525 conj viability,calpain
R3911 T14529 T14523 aux has,noted
R3912 T14398 T14396 pobj viability,of
R3913 T14530 T14523 auxpass been,noted
R3914 T14531 T14523 prep in,noted
R3915 T14532 T14533 nmod Capn4,MEFs
R3916 T14533 T14531 pobj MEFs,in
R3917 T14534 T14532 punct -,Capn4
R3918 T14535 T14532 punct /,Capn4
R3919 T14536 T14532 punct -,Capn4
R3920 T14399 T14385 punct ", ",is
R3921 T14537 T14533 cc and,MEFs
R3922 T14538 T14539 amod other,lines
R3923 T14400 T14401 det the,survival
R3924 T14401 T14385 nsubj survival,is
R3925 T14539 T14533 conj lines,MEFs
R3926 T14540 T14539 compound cell,lines
R3927 T14541 T14523 punct ", ",noted
R3928 T14402 T14401 prep of,survival
R3929 T14542 T14543 mark although,distinguish
R3930 T14543 T14523 advcl distinguish,noted
R3931 T14403 T14404 det a,embryos
R3932 T14544 T14545 det the,work
R3933 T14545 T14543 nsubj work,distinguish
R3934 T14404 T14402 pobj embryos,of
R3935 T14546 T14545 amod reported,work
R3936 T14547 T14543 advmod frequently,distinguish
R3937 T14405 T14404 amod few,embryos
R3938 T14548 T14543 aux did,distinguish
R3939 T14549 T14543 neg not,distinguish
R3940 T14550 T14543 prep between,distinguish
R3941 T14406 T14404 nmod Capn2,embryos
R3942 T14551 T14552 nmod μ,calpain
R3943 T14552 T14550 pobj calpain,between
R3944 T14407 T14406 punct -,Capn2
R3945 T14553 T14551 punct -,μ
R3946 T14554 T14551 cc and,μ
R3947 T14408 T14406 punct /,Capn2
R3948 T14555 T14551 conj m,μ
R3949 T14556 T14552 punct -,calpain
R3950 T14557 T14543 punct ", ",distinguish
R3951 T14409 T14406 punct -,Capn2
R3952 T14558 T14543 cc and,distinguish
R3953 T14559 T14560 aux did,show
R3954 T14410 T14401 prep to,survival
R3955 T14560 T14543 conj show,distinguish
R3956 T14561 T14560 neg not,show
R3957 T14562 T14563 mark that,was
R3958 T14411 T14412 det the,stage
R3959 T14563 T14560 ccomp was,show
R3960 T14564 T14563 nsubj calpain,was
R3961 T14565 T14566 advmod strictly,essential
R3962 T14412 T14410 pobj stage,to
R3963 T14566 T14563 acomp essential,was
R3964 T14567 T14568 punct [,6
R3965 T14568 T14560 parataxis 6,show
R3966 T14569 T14568 nummod 5,6
R3967 T14413 T14414 nummod 8,cell
R3968 T14414 T14412 compound cell,stage
R3969 T14570 T14568 punct ",",6
R3970 T14571 T14568 punct ],6
R3971 T14572 T14523 punct .,noted
R3972 T14415 T14414 punct -,cell
R3973 T14574 T14575 prep In,shown
R3974 T14416 T14417 advmod most,likely
R3975 T14576 T14577 preconj both,cells
R3976 T14577 T14574 pobj cells,In
R3977 T14417 T14418 advmod likely,due
R3978 T14578 T14577 compound CHO,cells
R3979 T14579 T14577 cc and,cells
R3980 T14580 T14581 nmod Capn4,MEFs
R3981 T14418 T14385 prep due,is
R3982 T14581 T14577 conj MEFs,cells
R3983 T14582 T14580 punct -,Capn4
R3984 T14583 T14580 punct /,Capn4
R3985 T14419 T14418 pcomp to,due
R3986 T14584 T14580 punct -,Capn4
R3987 T14585 T14575 punct ", ",shown
R3988 T14420 T14421 det the,persistence
R3989 T14586 T14575 nsubjpass calpain,shown
R3990 T14587 T14575 auxpass was,shown
R3991 T14588 T14589 aux to,influence
R3992 T14421 T14418 pobj persistence,due
R3993 T14589 T14575 xcomp influence,shown
R3994 T14590 T14591 compound cell,proliferation
R3995 T14591 T14589 dobj proliferation,influence
R3996 T14422 T14421 prep of,persistence
R3997 T14592 T14589 punct ", ",influence
R3998 T14593 T14594 cc but,at
R3999 T14594 T14589 prep at,influence
R4000 T14423 T14424 det some,calpain
R4001 T14595 T14594 advmod only,at
R4002 T14596 T14597 advmod very,low
R4003 T14597 T14598 amod low,densities
R4004 T14424 T14422 pobj calpain,of
R4005 T14598 T14594 pobj densities,at
R4006 T14599 T14598 compound cell,densities
R4007 T14600 T14601 punct [,5
R4008 T14425 T14424 amod maternal,calpain
R4009 T14601 T14589 parataxis 5,influence
R4010 T14602 T14601 punct ],5
R4011 T14426 T14424 compound m,calpain
R4012 T14603 T14575 punct .,shown
R4013 T14605 T14606 nsubjpass Calpain,associated
R4014 T14427 T14424 punct -,calpain
R4015 T14607 T14606 aux has,associated
R4016 T14428 T14424 appos mRNA,calpain
R4017 T14608 T14606 advmod also,associated
R4018 T14609 T14606 auxpass been,associated
R4019 T14610 T14606 prep with,associated
R4020 T14611 T14610 pobj progression,with
R4021 T14612 T14611 prep through,progression
R4022 T14429 T14428 cc and,mRNA
R4023 T14613 T14614 det the,stage
R4024 T14614 T14612 pobj stage,through
R4025 T14615 T14614 compound G1,stage
R4026 T14430 T14429 punct /,and
R4027 T14616 T14614 prep of,stage
R4028 T14617 T14618 det the,cycle
R4029 T14431 T14429 cc or,and
R4030 T14618 T14616 pobj cycle,of
R4031 T14619 T14618 compound cell,cycle
R4032 T14620 T14621 punct [,6
R4033 T14432 T14428 conj protein,mRNA
R4034 T14621 T14606 parataxis 6,associated
R4035 T14622 T14621 punct ],6
R4036 T14623 T14606 punct .,associated
R4037 T14433 T14421 prep through,persistence
R4038 T14434 T14435 quantmod 2,3
R4039 T14625 T14626 advmod Furthermore,reputed
R4040 T14435 T14437 nummod 3,divisions
R4041 T14627 T14626 punct ", ",reputed
R4042 T14436 T14435 punct –,3
R4043 T14628 T14626 nsubjpass some,reputed
R4044 T14629 T14628 prep of,some
R4045 T14630 T14631 det the,proteins
R4046 T14437 T14433 pobj divisions,through
R4047 T14631 T14629 pobj proteins,of
R4048 T14632 T14631 acl known,proteins
R4049 T14438 T14437 compound cell,divisions
R4050 T14633 T14634 aux to,involved
R4051 T14634 T14632 xcomp involved,known
R4052 T14635 T14634 auxpass be,involved
R4053 T14439 T14385 punct .,is
R4054 T14636 T14634 prep in,involved
R4055 T14637 T14638 compound cell,cycle
R4056 T14638 T14639 compound cycle,progression
R4057 T14441 T14442 det The,reasons
R4058 T14639 T14636 pobj progression,in
R4059 T14640 T14631 punct ", ",proteins
R4060 T14641 T14642 amod such,as
R4061 T14642 T14631 prep as,proteins
R4062 T14442 T14444 nsubj reasons,are
R4063 T14643 T14642 pobj p53,as
R4064 T14644 T14643 punct ", ",p53
R4065 T14645 T14643 conj p107,p53
R4066 T14443 T14442 amod mechanistic,reasons
R4067 T14646 T14645 punct ", ",p107
R4068 T14647 T14648 compound cyclin,D1
R4069 T14445 T14442 prep for,reasons
R4070 T14648 T14645 conj D1,p107
R4071 T14649 T14648 punct ", ",D1
R4072 T14650 T14648 cc and,D1
R4073 T14651 T14648 conj p27kip1,D1
R4074 T14652 T14626 auxpass are,reputed
R4075 T14653 T14654 aux to,be
R4076 T14446 T14445 pobj lethality,for
R4077 T14654 T14626 xcomp be,reputed
R4078 T14655 T14656 compound calpain,substrates
R4079 T14656 T14654 attr substrates,be
R4080 T14447 T14442 prep in,reasons
R4081 T14657 T14658 punct [,3
R4082 T14658 T14654 parataxis 3,be
R4083 T14659 T14658 punct ],3
R4084 T14448 T14449 det the,absence
R4085 T14660 T14626 punct .,reputed
R4086 T14662 T14663 compound Sperm,binding
R4087 T14449 T14447 pobj absence,in
R4088 T14663 T14664 nsubj binding,leads
R4089 T14450 T14449 prep of,absence
R4090 T14665 T14663 prep to,binding
R4091 T14666 T14667 det the,oocyte
R4092 T14667 T14665 pobj oocyte,to
R4093 T14451 T14452 compound m,calpain
R4094 T14668 T14664 prep to,leads
R4095 T14669 T14670 amod increased,calcium
R4096 T14670 T14668 pobj calcium,to
R4097 T14452 T14450 pobj calpain,of
R4098 T14671 T14670 amod cytoplasmic,calcium
R4099 T14672 T14673 dep which,triggers
R4100 T14673 T14670 relcl triggers,calcium
R4101 T14453 T14452 punct -,calpain
R4102 T14674 T14675 det the,reaction
R4103 T14675 T14673 dobj reaction,triggers
R4104 T14454 T14444 advmod still,are
R4105 T14455 T14444 acomp unclear,are
R4106 T14676 T14675 compound acrosome,reaction
R4107 T14456 T14444 punct .,are
R4108 T14677 T14678 punct [,46
R4109 T14678 T14664 parataxis 46,leads
R4110 T14679 T14678 punct ],46
R4111 T14458 T14459 expl There,been
R4112 T14680 T14664 punct .,leads
R4113 T14682 T14683 preconj Both,μ
R4114 T14683 T14684 nmod μ,calpain
R4115 T14460 T14459 aux have,been
R4116 T14684 T14689 nsubjpass calpain,detected
R4117 T14685 T14683 punct -,μ
R4118 T14686 T14683 cc and,μ
R4119 T14687 T14683 conj m,μ
R4120 T14461 T14462 amod several,reports
R4121 T14688 T14684 punct -,calpain
R4122 T14689 T14693 ccomp detected,implicated
R4123 T14690 T14689 aux have,detected
R4124 T14691 T14689 advmod recently,detected
R4125 T14692 T14689 auxpass been,detected
R4126 T14462 T14459 attr reports,been
R4127 T14694 T14689 prep in,detected
R4128 T14695 T14696 compound rodent,sperm
R4129 T14696 T14694 pobj sperm,in
R4130 T14463 T14462 prep of,reports
R4131 T14697 T14698 punct [,47
R4132 T14698 T14696 parataxis 47,sperm
R4133 T14699 T14698 punct ],47
R4134 T14464 T14465 compound m,calpain
R4135 T14700 T14696 cc and,sperm
R4136 T14701 T14696 conj oocytes,sperm
R4137 T14702 T14703 punct [,45
R4138 T14781 T14782 amod defective,segregation
R4139 T14703 T14701 parataxis 45,oocytes
R4140 T14704 T14703 punct ],45
R4141 T14705 T14689 punct .,detected
R4142 T14706 T14707 compound m,calpain
R4143 T14782 T14778 conj segregation,Polyspermy
R4144 T14707 T14693 nsubjpass calpain,implicated
R4145 T14708 T14707 punct -,calpain
R4146 T14709 T14693 auxpass was,implicated
R4147 T14783 T14782 compound chromosome,segregation
R4148 T14784 T14779 aux would,have
R4149 T14710 T14693 prep in,implicated
R4150 T14711 T14712 det the,reaction
R4151 T14712 T14710 pobj reaction,in
R4152 T14785 T14779 advmod both,have
R4153 T14713 T14712 compound acrosome,reaction
R4154 T14714 T14715 punct [,47
R4155 T14786 T14787 amod lethal,effects
R4156 T14715 T14712 parataxis 47,reaction
R4157 T14716 T14715 punct ],47
R4158 T14717 T14718 dep which,correlated
R4159 T14718 T14712 relcl correlated,reaction
R4160 T14787 T14779 dobj effects,have
R4161 T14719 T14718 prep with,correlated
R4162 T14720 T14721 det a,translocation
R4163 T14788 T14787 prep on,effects
R4164 T14721 T14719 pobj translocation,with
R4165 T14722 T14721 prep of,translocation
R4166 T14789 T14790 amod early,development
R4167 T14723 T14724 compound m,calpain
R4168 T14724 T14722 pobj calpain,of
R4169 T14725 T14724 punct -,calpain
R4170 T14790 T14788 pobj development,on
R4171 T14726 T14721 prep to,translocation
R4172 T14727 T14728 det the,membrane
R4173 T14728 T14726 pobj membrane,to
R4174 T14729 T14728 amod cortical,membrane
R4175 T14730 T14728 prep in,membrane
R4176 T14731 T14730 pobj oocytes,in
R4177 T14791 T14790 amod embryonic,development
R4178 T14732 T14733 advmod where,participate
R4179 T14733 T14728 relcl participate,membrane
R4180 T14734 T14733 nsubj it,participate
R4181 T14792 T14779 punct .,have
R4182 T14735 T14733 aux might,participate
R4183 T14736 T14733 prep in,participate
R4184 T14737 T14738 det the,release
R4185 T14794 T14795 nsubjpass It,stressed
R4186 T14738 T14736 pobj release,in
R4187 T14739 T14738 prep of,release
R4188 T14740 T14741 amod cortical,contents
R4189 T14741 T14739 pobj contents,of
R4190 T14796 T14795 aux should,stressed
R4191 T14742 T14741 compound granule,contents
R4192 T14743 T14741 acl required,contents
R4193 T14744 T14745 aux to,prevent
R4194 T14745 T14743 advcl prevent,required
R4195 T14797 T14795 advmod also,stressed
R4196 T14746 T14745 dobj polyspermy,prevent
R4197 T14747 T14748 punct [,45
R4198 T14748 T14745 parataxis 45,prevent
R4199 T14749 T14748 punct ],45
R4200 T14750 T14693 punct .,implicated
R4201 T14798 T14795 auxpass be,stressed
R4202 T14752 T14753 compound m,calpain
R4203 T14753 T14755 nsubj calpain,relocalized
R4204 T14754 T14753 punct -,calpain
R4205 T14799 T14800 mark that,maintained
R4206 T14756 T14755 advmod also,relocalized
R4207 T14800 T14795 ccomp maintained,stressed
R4208 T14757 T14755 prep to,relocalized
R4209 T14758 T14759 det the,spindle
R4210 T14759 T14757 pobj spindle,to
R4211 T14801 T14802 nmod Capn4,cells
R4212 T14760 T14759 nmod oocyte,spindle
R4213 T14761 T14759 amod meiotic,spindle
R4214 T14762 T14755 prep after,relocalized
R4215 T14802 T14800 nsubjpass cells,maintained
R4216 T14763 T14762 pobj fertilization,after
R4217 T14764 T14755 punct ", ",relocalized
R4218 T14803 T14801 punct -,Capn4
R4219 T14765 T14766 dep were,involved
R4220 T14766 T14755 ccomp involved,relocalized
R4221 T14767 T14766 nsubjpass it,involved
R4222 T14804 T14801 punct /,Capn4
R4223 T14768 T14766 aux could,involved
R4224 T14769 T14766 auxpass be,involved
R4225 T14770 T14766 prep in,involved
R4226 T14771 T14772 compound chromosome,segregation
R4227 T14772 T14770 pobj segregation,in
R4228 T14773 T14774 punct [,45
R4229 T14805 T14801 punct -,Capn4
R4230 T14774 T14766 parataxis 45,involved
R4231 T14775 T14774 punct ],45
R4232 T14776 T14755 punct .,relocalized
R4233 T14806 T14802 nmod ES,cells
R4234 T14778 T14779 nsubj Polyspermy,have
R4235 T14807 T14806 cc and,ES
R4236 T14780 T14778 cc or,Polyspermy
R4237 T14808 T14806 conj MEF,ES
R4238 T14809 T14802 prep from,cells
R4239 T14810 T14811 det the,allele
R4240 T14811 T14809 pobj allele,from
R4241 T14812 T14811 amod presumptive,allele
R4242 T14888 T14887 punct ", ",exist
R4243 T14813 T14811 amod hypomorphic,allele
R4244 T14889 T14890 npadvmod calpain,independent
R4245 T14814 T14800 aux can,maintained
R4246 T14890 T14892 amod independent,mechanisms
R4247 T14891 T14890 punct -,independent
R4248 T14892 T14887 nsubj mechanisms,exist
R4249 T14815 T14800 auxpass be,maintained
R4250 T14893 T14892 prep for,mechanisms
R4251 T14894 T14893 pcomp protecting,for
R4252 T14816 T14800 prep in,maintained
R4253 T14895 T14896 compound cell,viability
R4254 T14896 T14894 dobj viability,protecting
R4255 T14817 T14816 pobj culture,in
R4256 T14897 T14887 aux might,exist
R4257 T14898 T14887 prep in,exist
R4258 T14899 T14900 det these,lines
R4259 T14818 T14800 prep despite,maintained
R4260 T14900 T14898 pobj lines,in
R4261 T14901 T14900 compound cell,lines
R4262 T14819 T14820 det an,lack
R4263 T14902 T14903 dep that,are
R4264 T14903 T14887 ccomp are,exist
R4265 T14904 T14903 acomp absent,are
R4266 T14820 T14818 pobj lack,despite
R4267 T14905 T14903 prep in,are
R4268 T14906 T14907 amod early,cells
R4269 T14821 T14820 amod apparent,lack
R4270 T14907 T14905 pobj cells,in
R4271 T14908 T14907 amod embryonic,cells
R4272 T14909 T14887 punct .,exist
R4273 T14822 T14820 prep of,lack
R4274 T14911 T14912 det The,failure
R4275 T14912 T14914 nsubj failure,suggests
R4276 T14823 T14824 compound calpain,activity
R4277 T14913 T14912 amod repeated,failure
R4278 T14915 T14916 aux to,achieve
R4279 T14916 T14912 acl achieve,failure
R4280 T14917 T14918 compound gene,conversion
R4281 T14824 T14822 pobj activity,of
R4282 T14918 T14916 dobj conversion,achieve
R4283 T14919 T14918 prep of,conversion
R4284 T14920 T14919 pobj Capn2,of
R4285 T14825 T14800 punct ", ",maintained
R4286 T14921 T14920 punct +,Capn2
R4287 T14922 T14920 punct /,Capn2
R4288 T14923 T14920 punct -,Capn2
R4289 T14826 T14827 mark as,assessed
R4290 T14924 T14918 prep to,conversion
R4291 T14925 T14924 pobj Capn2,to
R4292 T14827 T14800 advcl assessed,maintained
R4293 T14926 T14925 punct -,Capn2
R4294 T14927 T14925 punct /,Capn2
R4295 T14928 T14925 punct -,Capn2
R4296 T14828 T14827 prep by,assessed
R4297 T14929 T14930 compound ES,cells
R4298 T14930 T14918 appos cells,conversion
R4299 T14829 T14830 compound casein,zymography
R4300 T14931 T14918 prep by,conversion
R4301 T14932 T14931 pobj selection,by
R4302 T14933 T14932 prep of,selection
R4303 T14830 T14828 pobj zymography,by
R4304 T14934 T14933 pobj clones,of
R4305 T14935 T14932 prep in,selection
R4306 T14831 T14828 cc or,by
R4307 T14936 T14937 amod high,concentrations
R4308 T14937 T14935 pobj concentrations,in
R4309 T14938 T14937 prep of,concentrations
R4310 T14832 T14828 conj by,by
R4311 T14939 T14938 pobj G418,of
R4312 T14940 T14941 mark that,compromises
R4313 T14833 T14834 det the,appearance
R4314 T14941 T14914 ccomp compromises,suggests
R4315 T14942 T14943 det the,state
R4316 T14943 T14941 nsubj state,compromises
R4317 T14834 T14832 pobj appearance,by
R4318 T14944 T14943 amod homozygous,state
R4319 T14945 T14943 compound mutant,state
R4320 T14946 T14941 advmod somehow,compromises
R4321 T14835 T14834 prep of,appearance
R4322 T14947 T14948 compound cell,viability
R4323 T14948 T14941 dobj viability,compromises
R4324 T14836 T14837 amod characteristic,products
R4325 T14949 T14948 cc or,viability
R4326 T14950 T14951 amod clonal,selection
R4327 T14951 T14948 conj selection,viability
R4328 T14837 T14835 pobj products,of
R4329 T14952 T14951 prep of,selection
R4330 T14953 T14954 compound ES,cells
R4331 T14954 T14952 pobj cells,of
R4332 T14955 T14914 punct .,suggests
R4333 T14838 T14837 compound spectrin,products
R4334 T14957 T14958 nsubj Efforts,are
R4335 T14959 T14960 advmod under,way
R4336 T14839 T14837 compound breakdown,products
R4337 T14960 T14958 advmod way,are
R4338 T14961 T14962 aux to,express
R4339 T14840 T14795 punct .,stressed
R4340 T14962 T14958 xcomp express,are
R4341 T14963 T14964 det the,subunit
R4342 T14842 T14843 nsubj It,is
R4343 T14844 T14843 acomp conceivable,is
R4344 T14964 T14962 dobj subunit,express
R4345 T14965 T14964 nmod m,subunit
R4346 T14966 T14965 punct -,m
R4347 T14845 T14843 punct ", ",is
R4348 T14967 T14965 nummod 80,m
R4349 T14968 T14964 compound k,subunit
R4350 T14969 T14964 prep from,subunit
R4351 T14846 T14847 mark as,discussed
R4352 T14970 T14971 det a,transgene
R4353 T14971 T14969 pobj transgene,from
R4354 T14847 T14843 advcl discussed,is
R4355 T14972 T14971 compound Capn2,transgene
R4356 T14973 T14971 compound cDNA,transgene
R4357 T14848 T14847 advmod earlier,discussed
R4358 T14974 T14971 compound rescue,transgene
R4359 T14975 T14976 amod prior,to
R4360 T14976 T14962 prep to,express
R4361 T14849 T14843 punct ", ",is
R4362 T14977 T14978 compound gene,conversion
R4363 T14978 T14976 pobj conversion,to
R4364 T14979 T14962 prep in,express
R4365 T14850 T14851 mark that,retained
R4366 T14980 T14979 pobj order,in
R4367 T14981 T14982 aux to,rescue
R4368 T14851 T14843 ccomp retained,is
R4369 T14982 T14980 acl rescue,order
R4370 T14983 T14982 advmod preemptively,rescue
R4371 T14984 T14985 compound m,calpain
R4372 T14852 T14853 det a,amount
R4373 T14985 T14987 compound calpain,activity
R4374 T14986 T14985 punct -,calpain
R4375 T14987 T14982 dobj activity,rescue
R4376 T14853 T14851 nsubjpass amount,retained
R4377 T14988 T14989 mark before,lost
R4378 T14989 T14982 advcl lost,rescue
R4379 T14990 T14991 det the,allele
R4380 T14854 T14853 compound trace,amount
R4381 T14991 T14989 nsubjpass allele,lost
R4382 T14992 T14991 amod remaining,allele
R4383 T14993 T14991 amod endogenous,allele
R4384 T14855 T14853 prep of,amount
R4385 T14856 T14857 compound calpain,activity
R4386 T14994 T14995 amod wild,type
R4387 T14995 T14991 compound type,allele
R4388 T14996 T14995 punct -,type
R4389 T14857 T14855 pobj activity,of
R4390 T14997 T14989 auxpass is,lost
R4391 T14998 T14958 punct .,are
R4392 T14858 T14853 punct ", ",amount
R4393 T15000 T15001 advmod However,hampered
R4394 T14859 T14853 prep beneath,amount
R4395 T15002 T15001 punct ", ",hampered
R4396 T15003 T15001 nsubj difficulty,hampered
R4397 T14860 T14859 pobj levels,beneath
R4398 T15004 T15003 prep in,difficulty
R4399 T15005 T15004 pcomp achieving,in
R4400 T15006 T15007 amod stable,expression
R4401 T14861 T14860 amod detectable,levels
R4402 T15007 T15005 dobj expression,achieving
R4403 T15008 T15007 prep of,expression
R4404 T15009 T15010 det the,transgene
R4405 T14862 T14861 prep by,detectable
R4406 T15010 T15008 pobj transgene,of
R4407 T15011 T15010 compound rescue,transgene
R4408 T14863 T14864 det these,methods
R4409 T15012 T15001 aux has,hampered
R4410 T15013 T15014 advmod thus,far
R4411 T15014 T15001 advmod far,hampered
R4412 T14864 T14862 pobj methods,by
R4413 T15015 T15016 det these,attempts
R4414 T15016 T15001 dobj attempts,hampered
R4415 T14865 T14851 punct ", ",retained
R4416 T15017 T15001 punct .,hampered
R4417 T14866 T14851 auxpass is,retained
R4418 T15019 T15020 nummod One,curiosity
R4419 T15020 T15021 nsubj curiosity,was
R4420 T14867 T14851 prep from,retained
R4421 T15022 T15023 dep which,arose
R4422 T15023 T15020 relcl arose,curiosity
R4423 T14868 T14869 det this,allele
R4424 T15024 T15023 prep during,arose
R4425 T15025 T15026 det the,genotyping
R4426 T15026 T15024 pobj genotyping,during
R4427 T14869 T14867 pobj allele,from
R4428 T15027 T15026 prep of,genotyping
R4429 T15028 T15027 pobj progeny,of
R4430 T15029 T15028 prep from,progeny
R4431 T14870 T14869 compound mutant,allele
R4432 T15030 T15031 amod heterozygous,interbreeding
R4433 T15031 T15029 pobj interbreeding,from
R4434 T15032 T15033 det the,ratio
R4435 T15033 T15021 attr ratio,was
R4436 T15034 T15035 advmod highly,non-Mendelian
R4437 T14871 T14869 compound Capn4,allele
R4438 T15035 T15033 amod non-Mendelian,ratio
R4439 T15036 T15033 prep of,ratio
R4440 T15037 T15036 pobj Capn2,of
R4441 T14872 T14843 punct ", ",is
R4442 T15038 T15037 punct +,Capn2
R4443 T15039 T15037 punct /,Capn2
R4444 T15040 T15037 punct +,Capn2
R4445 T14873 T14843 cc and,is
R4446 T15041 T15042 punct (,%
R4447 T15042 T15037 parataxis %,Capn2
R4448 T15043 T15042 nummod 11.6,%
R4449 T14874 T14875 nsubj it,is
R4450 T15044 T15042 punct ),%
R4451 T15045 T15033 prep to,ratio
R4452 T14875 T14843 conj is,is
R4453 T15046 T15045 pobj Capn2,to
R4454 T15047 T15046 punct +,Capn2
R4455 T15048 T15046 punct /,Capn2
R4456 T14876 T14875 acomp sufficient,is
R4457 T15049 T15046 punct -,Capn2
R4458 T14877 T14876 prep for,sufficient
R4459 T15050 T15051 punct (,%
R4460 T14878 T14877 pcomp maintaining,for
R4461 T15051 T15046 parataxis %,Capn2
R4462 T15052 T15051 nummod 88.4,%
R4463 T14879 T14880 det the,viability
R4464 T15053 T15051 punct ),%
R4465 T15054 T15033 prep of,ratio
R4466 T15055 T15054 pobj weanlings,of
R4467 T14880 T14878 dobj viability,maintaining
R4468 T15056 T15021 punct .,was
R4469 T15058 T15059 prep At,is
R4470 T14881 T14880 prep of,viability
R4471 T15060 T15058 amod present,At
R4472 T14882 T14883 det the,cells
R4473 T15061 T15059 punct ", ",is
R4474 T15062 T15059 expl there,is
R4475 T14883 T14881 pobj cells,of
R4476 T15063 T15064 det no,explanation
R4477 T15064 T15059 attr explanation,is
R4478 T15065 T15064 amod obvious,explanation
R4479 T14884 T14875 punct .,is
R4480 T15066 T15064 prep for,explanation
R4481 T15067 T15068 det this,result
R4482 T14886 T14887 advmod Furthermore,exist
R4483 T15068 T15066 pobj result,for
R4484 T15069 T15059 punct .,is
R4485 T15071 T15072 nsubj Crosses,produced
R4486 T15073 T15071 prep between,Crosses
R4487 T15074 T15075 amod wild,type
R4488 T15100 T15098 pobj line,involving
R4489 T15075 T15077 nmod type,mice
R4490 T15076 T15075 punct -,type
R4491 T15077 T15073 pobj mice,between
R4492 T15078 T15075 cc and,type
R4493 T15079 T15075 conj heterozygous,type
R4494 T15080 T15072 advmod also,produced
R4495 T15101 T15100 nmod Capn4,line
R4496 T15081 T15072 dobj progeny,produced
R4497 T15082 T15081 prep with,progeny
R4498 T15083 T15084 det a,proportion
R4499 T15084 T15082 pobj proportion,with
R4500 T15102 T15100 amod transgenic,line
R4501 T15085 T15084 amod greater,proportion
R4502 T15103 T15100 acl generated,line
R4503 T15086 T15087 mark than,expected
R4504 T15087 T15085 advcl expected,greater
R4505 T15104 T15103 agent by,generated
R4506 T15088 T15084 prep of,proportion
R4507 T15089 T15090 amod heterozygous,offspring
R4508 T15105 T15104 pobj Zimmerman,by
R4509 T15090 T15088 pobj offspring,of
R4510 T15091 T15072 punct .,produced
R4511 T15106 T15105 cc and,Zimmerman
R4512 T15093 T15094 advmod Interestingly,yielded
R4513 T15107 T15105 conj colleagues,Zimmerman
R4514 T15095 T15094 punct ", ",yielded
R4515 T15096 T15097 amod heterozygous,crosses
R4516 T15097 T15094 nsubj crosses,yielded
R4517 T15108 T15094 dobj progeny,yielded
R4518 T15098 T15097 prep involving,crosses
R4519 T15099 T15100 det the,line
R4520 T15109 T15108 prep with,progeny
R4521 T15110 T15111 det a,skewing
R4522 T15111 T15109 pobj skewing,with
R4523 T15112 T15111 amod similar,skewing
R4524 T15113 T15114 punct ", ",extreme
R4525 T15114 T15111 parataxis extreme,skewing
R4526 T15115 T15114 mark if,extreme
R4527 T15116 T15114 advmod less,extreme
R4528 T15117 T15114 punct ", ",extreme
R4529 T15118 T15111 prep in,skewing
R4530 T15119 T15118 pobj favor,in
R4531 T15120 T15119 prep of,favor
R4532 T15121 T15122 det the,genotype
R4533 T15122 T15120 pobj genotype,of
R4534 T15123 T15122 amod heterozygous,genotype
R4535 T15124 T15094 punct .,yielded
R4536 T15126 T15127 prep Out,were
R4537 T15128 T15126 prep of,Out
R4538 T15129 T15130 det a,total
R4539 T15130 T15128 pobj total,of
R4540 T15131 T15130 prep of,total
R4541 T15132 T15133 nummod 80,animals
R4542 T15133 T15131 pobj animals,of
R4543 T15134 T15133 amod genotyped,animals
R4544 T15135 T15127 punct ", ",were
R4545 T15136 T15137 nummod 22.5,%
R4546 T15137 T15127 nsubj %,were
R4589 T15430 T15431 det The,work
R4590 T15431 T15432 nsubj work,clarified
R4591 T15433 T15431 acl presented,work
R4592 T15434 T15433 advmod here,presented
R4593 T15435 T15432 aux has,clarified
R4594 T15436 T15437 nummod two,questions
R4595 T15437 T15432 dobj questions,clarified
R4596 T15438 T15437 amod important,questions
R4597 T15439 T15437 prep regarding,questions
R4598 T15440 T15441 det the,roles
R4599 T15441 T15439 pobj roles,regarding
R4600 T15442 T15441 amod physiological,roles
R4601 T15443 T15441 prep of,roles
R4602 T15444 T15445 det the,isoforms
R4603 T15445 T15443 pobj isoforms,of
R4604 T15446 T15445 nummod two,isoforms
R4605 T15447 T15445 amod ubiquitous,isoforms
R4606 T15448 T15445 compound calpain,isoforms
R4607 T15449 T15445 punct ", ",isoforms
R4608 T15450 T15451 nmod μ,calpain
R4609 T15451 T15445 appos calpain,isoforms
R4610 T15452 T15450 punct -,μ
R4611 T15453 T15450 cc and,μ
R4612 T15454 T15450 conj m,μ
R4613 T15455 T15451 punct -,calpain
R4614 T15456 T15432 punct .,clarified
R4615 T15458 T15459 advmod Firstly,determined
R4616 T15460 T15459 punct ", ",determined
R4617 T15461 T15459 nsubjpass it,determined
R4618 T15462 T15459 auxpass was,determined
R4619 T15463 T15464 mark that,plays
R4620 T15464 T15459 ccomp plays,determined
R4621 T15465 T15466 compound m,calpain
R4622 T15466 T15464 nsubj calpain,plays
R4623 T15467 T15466 punct -,calpain
R4624 T15468 T15469 det an,role
R4625 T15469 T15464 dobj role,plays
R4626 T15470 T15469 amod indispensable,role
R4627 T15471 T15469 prep in,role
R4628 T15472 T15473 amod murine,embryogenesis
R4629 T15473 T15471 pobj embryogenesis,in
R4630 T15474 T15469 punct ", ",role
R4631 T15475 T15476 advmod possibly,related
R4632 T15476 T15469 acl related,role
R4633 T15477 T15476 prep to,related
R4634 T15478 T15479 amod pre-implantation,development
R4635 T15479 T15477 pobj development,to
R4636 T15480 T15459 punct .,determined
R4637 T15482 T15483 advmod Furthermore,carried
R4638 T15484 T15483 punct ", ",carried
R4639 T15485 T15486 det this,function
R4640 T15486 T15483 nsubjpass function,carried
R4641 T15487 T15483 aux can,carried
R4642 T15488 T15483 neg not,carried
R4643 T15489 T15483 auxpass be,carried
R4644 T15490 T15483 prt out,carried
R4645 T15491 T15483 agent by,carried
R4646 T15492 T15493 compound μ,calpain
R4647 T15493 T15491 pobj calpain,by
R4648 T15494 T15493 punct -,calpain
R4649 T15495 T15483 prep despite,carried
R4650 T15496 T15497 det the,similarities
R4651 T15497 T15495 pobj similarities,despite
R4652 T15498 T15497 amod apparent,similarities
R4653 T15499 T15500 advmod in,vitro
R4654 T15500 T15497 amod vitro,similarities
R4655 T15501 T15497 prep of,similarities
R4656 T15502 T15503 nmod μ,calpain
R4657 T15503 T15501 pobj calpain,of
R4658 T15504 T15502 punct -,μ
R4659 T15505 T15502 cc and,μ
R4660 T15506 T15502 conj m,μ
R4661 T15507 T15503 punct -,calpain
R4662 T15508 T15483 punct ", ",carried
R4663 T15509 T15483 advcl demonstrating,carried
R4664 T15510 T15511 mark that,have
R4665 T15511 T15509 ccomp have,demonstrating
R4666 T15512 T15513 det the,isoforms
R4667 T15513 T15511 nsubj isoforms,have
R4668 T15514 T15513 nummod two,isoforms
R4669 T15515 T15511 advmod clearly,have
R4670 T15516 T15517 det some,roles
R4671 T15517 T15511 dobj roles,have
R4672 T15518 T15517 amod distinct,roles
R4673 T15519 T15520 advmod in,vivo
R4674 T15520 T15511 advmod vivo,have
R4675 T15521 T15483 punct .,carried
R4676 T15523 T15524 det The,functions
R4677 T15524 T15525 nsubj functions,remain
R4678 T15526 T15524 prep of,functions
R4679 T15527 T15528 compound m,calpain
R4680 T15528 T15526 pobj calpain,of
R4681 T15529 T15528 punct -,calpain
R4682 T15530 T15524 prep during,functions
R4683 T15531 T15532 amod post-implantation,embryogenesis
R4684 T15532 T15530 pobj embryogenesis,during
R4685 T15533 T15530 cc and,during
R4686 T15534 T15530 conj in,during
R4687 T15535 T15536 amod adult,mice
R4688 T15536 T15534 pobj mice,in
R4689 T15537 T15538 aux to,elucidated
R4690 T15538 T15525 xcomp elucidated,remain
R4691 T15539 T15538 auxpass be,elucidated
R4692 T15540 T15525 cc and,remain
R4693 T15541 T15542 aux will,have
R4694 T15542 T15525 conj have,remain
R4695 T15543 T15544 aux to,addressed
R4696 T15544 T15542 xcomp addressed,have
R4697 T15545 T15544 auxpass be,addressed
R4698 T15546 T15544 advcl using,addressed
R4699 T15547 T15548 det a,strategy
R4700 T15548 T15546 dobj strategy,using
R4701 T15549 T15548 amod conditional,strategy
R4702 T15550 T15551 compound gene,targeting
R4703 T15551 T15548 compound targeting,strategy
R4704 T15552 T15525 punct .,remain
R4705 T16009 T16008 cc and,Cloning
R4706 T16010 T16008 conj sequencing,Cloning
R4707 T16011 T16008 prep of,Cloning
R4708 T16012 T16013 det the,locus
R4709 T16013 T16011 pobj locus,of
R4710 T16014 T16013 compound mouse,locus
R4711 T16015 T16013 compound Capn2,locus
R4712 T16017 T16018 det The,gene
R4713 T16018 T16021 nsubj gene,encodes
R4714 T16019 T16018 compound mouse,gene
R4715 T16020 T16018 compound Capn2,gene
R4716 T16022 T16023 det the,acids
R4717 T16023 T16021 dobj acids,encodes
R4718 T16024 T16023 nummod 700,acids
R4719 T16025 T16023 compound amino,acids
R4720 T16026 T16023 prep of,acids
R4721 T16027 T16028 det the,subunit
R4722 T16028 T16026 pobj subunit,of
R4723 T16029 T16030 nmod m,calpain
R4724 T16030 T16028 nmod calpain,subunit
R4725 T16031 T16030 punct -,calpain
R4726 T16032 T16028 amod large,subunit
R4727 T16033 T16034 punct (,k
R4728 T16034 T16028 parataxis k,subunit
R4729 T16035 T16034 nmod m,k
R4730 T16036 T16034 punct -,k
R4731 T16037 T16034 nummod 80,k
R4732 T16038 T16034 punct ),k
R4733 T16039 T16021 cc and,encodes
R4734 T16040 T16021 conj consists,encodes
R4735 T16041 T16040 prep of,consists
R4736 T16042 T16043 nummod 21,exons
R4737 T16043 T16041 pobj exons,of
R4738 T16044 T16043 acl extending,exons
R4739 T16045 T16044 prep over,extending
R4740 T16046 T16047 nummod 50,kb
R4741 T16047 T16045 pobj kb,over
R4742 T16048 T16047 punct -,kb
R4743 T16049 T16044 prep on,extending
R4744 T16050 T16049 pobj chromosome,on
R4745 T16051 T16050 nummod 1,chromosome
R4746 T16052 T16021 punct .,encodes
R4747 T16054 T16055 det A,clone
R4748 T16055 T16057 nsubjpass clone,purchased
R4749 T16056 T16055 compound cDNA,clone
R4750 T16058 T16055 acl encoding,clone
R4751 T16059 T16060 det a,portion
R4752 T16060 T16058 dobj portion,encoding
R4753 T16061 T16060 prep of,portion
R4754 T16062 T16063 det the,subunit
R4755 T16063 T16061 pobj subunit,of
R4756 T16064 T16063 nmod mouse,subunit
R4757 T16065 T16066 nmod m,k
R4758 T16066 T16063 compound k,subunit
R4759 T16067 T16066 punct -,k
R4760 T16068 T16066 nummod 80,k
R4761 T16069 T16057 auxpass was,purchased
R4762 T16070 T16071 punct (,LLNL
R4763 T16071 T16057 parataxis LLNL,purchased
R4764 T16072 T16073 compound dbEST,Id
R4765 T16073 T16071 dep Id,LLNL
R4766 T16074 T16071 punct ", ",LLNL
R4767 T16075 T16071 dep 807416,LLNL
R4768 T16076 T16071 punct ", ",LLNL
R4769 T16077 T16071 dep Image,LLNL
R4770 T16078 T16071 punct :,LLNL
R4771 T16079 T16071 dep 606689,LLNL
R4772 T16080 T16071 punct ", ",LLNL
R4773 T16081 T16082 compound Image,Consortium
R4774 T16082 T16071 dep Consortium,LLNL
R4775 T16083 T16071 punct ", ",LLNL
R4776 T16084 T16071 punct ),LLNL
R4777 T16085 T16057 punct .,purchased
R4778 T16087 T16088 nsubjpass It,found
R4779 T16089 T16088 auxpass was,found
R4780 T16090 T16091 aux to,contain
R4781 T16091 T16088 xcomp contain,found
R4782 T16092 T16093 nummod 2.8,kb
R4783 T16093 T16091 dobj kb,contain
R4784 T16094 T16093 punct -,kb
R4785 T16095 T16093 prep of,kb
R4786 T16096 T16095 pobj sequence,of
R4787 T16097 T16096 prep from,sequence
R4788 T16098 T16097 pobj position,from
R4789 T16099 T16098 nummod 247,position
R4790 T16100 T16098 prep of,position
R4791 T16101 T16102 det the,sequence
R4792 T16102 T16100 pobj sequence,of
R4793 T16103 T16102 compound coding,sequence
R4794 T16104 T16097 prep to,from
R4795 T16105 T16106 det the,codon
R4796 T16106 T16104 pobj codon,to
R4797 T16107 T16106 compound stop,codon
R4798 T16108 T16106 prep at,codon
R4799 T16109 T16108 pobj position,at
R4800 T16110 T16109 nummod "2,101",position
R4801 T16111 T16096 punct ", ",sequence
R4802 T16112 T16096 prep including,sequence
R4803 T16113 T16114 det the,UTR
R4804 T16114 T16112 pobj UTR,including
R4805 T16115 T16114 nummod 3,UTR
R4806 T16116 T16115 punct ',3
R4807 T16117 T16114 punct -,UTR
R4808 T16118 T16114 prep to,UTR
R4809 T16119 T16120 det the,signal
R4810 T16120 T16118 pobj signal,to
R4811 T16121 T16120 compound polyA,signal
R4812 T16122 T16088 punct .,found
R4813 T16124 T16125 det A,fragment
R4814 T16125 T16126 nsubjpass fragment,subcloned
R4815 T16127 T16125 prep of,fragment
R4816 T16128 T16129 det this,cDNA
R4817 T16129 T16127 pobj cDNA,of
R4818 T16130 T16126 auxpass was,subcloned
R4819 T16131 T16126 cc and,subcloned
R4820 T16132 T16126 conj used,subcloned
R4821 T16133 T16134 aux to,screen
R4822 T16134 T16132 advcl screen,used
R4823 T16135 T16136 det a,library
R4824 T16136 T16134 dobj library,screen
R4825 T16137 T16136 nummod 129Sv,library
R4826 T16138 T16136 nmod mouse,library
R4827 T16139 T16136 amod genomic,library
R4828 T16140 T16134 prep in,screen
R4829 T16141 T16142 compound λ,Dash
R4830 T16142 T16140 pobj Dash,in
R4831 T16143 T16142 punct -,Dash
R4832 T16144 T16142 nummod II,Dash
R4833 T16145 T16126 punct .,subcloned
R4834 T16147 T16148 amod Overlapping,clones
R4835 T16148 T16150 nsubjpass clones,obtained
R4836 T16149 T16148 amod genomic,clones
R4837 T16151 T16150 auxpass were,obtained
R4838 T16152 T16150 advcl covering,obtained
R4839 T16153 T16154 nummod "15,354",bp
R4840 T16154 T16152 dobj bp,covering
R4841 T16155 T16150 punct ", ",obtained
R4842 T16156 T16150 advcl extending,obtained
R4843 T16157 T16156 prep from,extending
R4844 T16158 T16159 det a,site
R4845 T16159 T16157 pobj site,from
R4846 T16160 T16159 compound BamHI,site
R4847 T16161 T16159 prep in,site
R4848 T16162 T16161 pobj intron,in
R4849 T16163 T16162 nummod 3,intron
R4850 T16164 T16157 prep to,from
R4851 T16165 T16166 det a,site
R4852 T16166 T16164 pobj site,to
R4853 T16167 T16166 compound BamHI,site
R4854 T16168 T16166 prep in,site
R4855 T16169 T16168 pobj intron,in
R4856 T16170 T16169 nummod 15,intron
R4857 T16171 T16150 punct .,obtained
R4858 T16173 T16174 det These,clones
R4859 T16174 T16175 nsubjpass clones,sequenced
R4860 T16176 T16175 auxpass were,sequenced
R4861 T16177 T16175 advmod completely,sequenced
R4862 T16178 T16175 prep on,sequenced
R4863 T16179 T16180 det both,strands
R4864 T16180 T16178 pobj strands,on
R4865 T16181 T16175 cc and,sequenced
R4866 T16182 T16183 det the,data
R4867 T16183 T16184 nsubjpass data,submitted
R4868 T16184 T16175 conj submitted,sequenced
R4869 T16185 T16184 auxpass were,submitted
R4870 T16186 T16184 prep to,submitted
R4871 T16187 T16186 pobj GenBank,to
R4872 T16188 T16189 punct (,AF497625
R4873 T16189 T16184 parataxis AF497625,submitted
R4874 T16190 T16189 compound accession,AF497625
R4875 T16191 T16189 compound no.,AF497625
R4876 T16192 T16189 punct ),AF497625
R4877 T16193 T16184 punct .,submitted
R4878 T16195 T16196 det The,sequences
R4879 T16196 T16198 nsubj sequences,agreed
R4880 T16197 T16196 amod submitted,sequences
R4881 T16199 T16198 advmod precisely,agreed
R4882 T16200 T16198 prep with,agreed
R4883 T16201 T16202 det the,databases
R4884 T16202 T16200 pobj databases,with
R4885 T16203 T16202 amod public,databases
R4886 T16204 T16198 punct ", ",agreed
R4887 T16205 T16198 cc and,agreed
R4888 T16206 T16207 advmod also,filled
R4889 T16207 T16198 conj filled,agreed
R4890 T16208 T16207 prt in,filled
R4891 T16209 T16210 amod several,gaps
R4892 T16210 T16207 dobj gaps,filled
R4893 T16211 T16210 amod small,gaps
R4894 T16212 T16210 acl corresponding,gaps
R4895 T16213 T16212 prep to,corresponding
R4896 T16214 T16215 amod short,sequences
R4897 T16215 T16213 pobj sequences,to
R4898 T16216 T16215 amod repetitive,sequences
R4899 T16217 T16218 dep which,established
R4900 T16218 T16215 relcl established,sequences
R4901 T16219 T16218 aux could,established
R4902 T16220 T16218 advmod only,established
R4903 T16221 T16218 auxpass be,established
R4904 T16222 T16218 advmod firmly,established
R4905 T16223 T16218 prep by,established
R4906 T16224 T16225 amod repeated,sequencing
R4907 T16225 T16223 pobj sequencing,by
R4908 T16226 T16225 prep in,sequencing
R4909 T16227 T16228 amod non-standard,conditions
R4910 T16228 T16226 pobj conditions,in
R4911 T16229 T16198 punct .,agreed
R4912 T16710 T16709 prep of,Construction
R4913 T16711 T16712 det the,vector
R4914 T16712 T16710 pobj vector,of
R4915 T16713 T16712 compound Capn2,vector
R4916 T16714 T16712 compound targeting,vector
R4917 T16716 T16717 det A,construct
R4918 T16717 T16719 nsubjpass construct,designed
R4919 T16718 T16717 compound targeting,construct
R4920 T16720 T16719 auxpass was,designed
R4921 T16721 T16722 aux to,replace
R4922 T16722 T16719 advcl replace,designed
R4923 T16723 T16724 det a,fragment
R4924 T16724 T16722 dobj fragment,replace
R4925 T16725 T16726 nummod 785,bp
R4926 T16726 T16724 compound bp,fragment
R4927 T16727 T16728 compound BamHI,HindIII
R4928 T16728 T16724 compound HindIII,fragment
R4929 T16729 T16728 punct -,HindIII
R4930 T16730 T16724 punct ", ",fragment
R4931 T16731 T16724 acl containing,fragment
R4932 T16732 T16731 dobj exon,containing
R4933 T16733 T16732 nummod 7,exon
R4934 T16734 T16732 prep of,exon
R4935 T16735 T16736 det the,gene
R4936 T16736 T16734 pobj gene,of
R4937 T16737 T16736 compound Capn2,gene
R4938 T16738 T16722 punct ", ",replace
R4939 T16739 T16722 prep with,replace
R4940 T16740 T16741 det the,cassette
R4941 T16741 T16739 pobj cassette,with
R4942 T16742 T16743 compound PGK,Neo
R4943 T16743 T16741 compound Neo,cassette
R4944 T16744 T16743 punct -,Neo
R4945 T16745 T16746 punct (,Figure
R4946 T16746 T16722 parataxis Figure,replace
R4947 T16747 T16746 nummod 1,Figure
R4948 T16748 T16746 punct ),Figure
R4949 T16749 T16719 punct .,designed
R4950 T16751 T16752 nsubj Exon,encodes
R4951 T16753 T16751 nummod 7,Exon
R4952 T16754 T16755 nummod 24,acids
R4953 T16755 T16752 dobj acids,encodes
R4954 T16756 T16755 compound amino,acids
R4955 T16757 T16752 prep in,encodes
R4956 T16758 T16759 det the,region
R4957 T16759 T16757 pobj region,in
R4958 T16760 T16761 amod active,site
R4959 T16761 T16759 compound site,region
R4960 T16762 T16761 punct -,site
R4961 T16763 T16752 punct ", ",encodes
R4962 T16764 T16752 prep including,encodes
R4963 T16765 T16764 pobj Asn286,including
R4964 T16766 T16765 punct ", ",Asn286
R4965 T16767 T16765 appos one,Asn286
R4966 T16768 T16767 prep of,one
R4967 T16769 T16770 det the,residues
R4968 T16770 T16768 pobj residues,of
R4969 T16771 T16770 amod catalytic,residues
R4970 T16772 T16770 compound triad,residues
R4971 T16773 T16752 punct .,encodes
R4972 T16775 T16776 det The,arm
R4973 T16776 T16781 nsubjpass arm,provided
R4974 T16777 T16776 amod short,arm
R4975 T16778 T16776 punct (,arm
R4976 T16779 T16776 amod upstream,arm
R4977 T16780 T16776 punct ),arm
R4978 T16782 T16776 prep of,arm
R4979 T16783 T16784 det the,construct
R4980 T16784 T16782 pobj construct,of
R4981 T16785 T16784 compound targeting,construct
R4982 T16786 T16781 auxpass was,provided
R4983 T16787 T16781 agent by,provided
R4984 T16788 T16789 det a,fragment
R4985 T16789 T16787 pobj fragment,by
R4986 T16790 T16791 nummod 2.7,kb
R4987 T16791 T16789 compound kb,fragment
R4988 T16792 T16791 punct -,kb
R4989 T16793 T16794 compound HindIII,BamHI
R4990 T16794 T16789 compound BamHI,fragment
R4991 T16795 T16794 punct -,BamHI
R4992 T16796 T16789 punct ", ",fragment
R4993 T16797 T16789 acl containing,fragment
R4994 T16798 T16799 nmod exons,5
R4995 T16799 T16797 dobj 5,containing
R4996 T16800 T16799 cc and,5
R4997 T16801 T16799 conj 6,5
R4998 T16802 T16789 punct ", ",fragment
R4999 T16803 T16804 dep which,inserted
R5000 T16804 T16789 relcl inserted,fragment
R5001 T16805 T16804 auxpass was,inserted
R5002 T16806 T16804 prep into,inserted
R5003 T16807 T16808 det the,vector
R5004 T16808 T16806 pobj vector,into
R5005 T16809 T16808 compound pPNT,vector
R5006 T16810 T16804 advmod upstream,inserted
R5007 T16811 T16810 prep of,upstream
R5008 T16812 T16813 det the,cassette
R5009 T16813 T16811 pobj cassette,of
R5010 T16814 T16815 compound PGK,Neo
R5011 T16815 T16813 compound Neo,cassette
R5012 T16816 T16815 punct -,Neo
R5013 T16817 T16818 punct [,48
R5014 T16818 T16804 parataxis 48,inserted
R5015 T16819 T16818 punct ],48
R5016 T16820 T16781 punct .,provided
R5017 T16822 T16823 prep During,abolished
R5018 T16824 T16822 pobj cloning,During
R5019 T16825 T16823 punct ", ",abolished
R5020 T16826 T16827 det the,site
R5021 T16827 T16823 nsubjpass site,abolished
R5022 T16828 T16827 compound BamHI,site
R5023 T16829 T16827 prep at,site
R5024 T16830 T16831 det the,end
R5025 T16831 T16829 pobj end,at
R5026 T16832 T16831 nummod 3,end
R5027 T16833 T16832 punct ',3
R5028 T16834 T16831 prep of,end
R5029 T16835 T16836 det the,arm
R5030 T16836 T16834 pobj arm,of
R5031 T16837 T16836 amod short,arm
R5032 T16838 T16823 auxpass was,abolished
R5033 T16839 T16823 punct .,abolished
R5034 T16841 T16842 det The,loss
R5035 T16842 T16843 nsubj loss,provided
R5036 T16844 T16842 prep of,loss
R5037 T16845 T16846 det this,site
R5038 T16846 T16844 pobj site,of
R5039 T16847 T16846 compound BamHI,site
R5040 T16848 T16842 prep in,loss
R5041 T16849 T16850 det the,allele
R5042 T16850 T16848 pobj allele,in
R5043 T16851 T16850 compound mutant,allele
R5044 T16852 T16842 punct ", ",loss
R5045 T16853 T16842 acl coupled,loss
R5046 T16854 T16853 prep with,coupled
R5047 T16855 T16856 det the,introduction
R5048 T16856 T16854 pobj introduction,with
R5049 T16857 T16856 prep of,introduction
R5050 T16858 T16859 det a,site
R5051 T16859 T16857 pobj site,of
R5052 T16860 T16859 amod new,site
R5053 T16861 T16859 compound BamHI,site
R5054 T16862 T16853 prep at,coupled
R5055 T16863 T16864 det the,end
R5056 T16864 T16862 pobj end,at
R5057 T16865 T16864 nummod 3,end
R5058 T16866 T16865 punct ',3
R5059 T16867 T16864 prep of,end
R5060 T16868 T16869 det the,cassette
R5061 T16869 T16867 pobj cassette,of
R5062 T16870 T16871 compound PGK,Neo
R5063 T16871 T16869 compound Neo,cassette
R5064 T16872 T16871 punct -,Neo
R5065 T16873 T16843 punct ", ",provided
R5066 T16874 T16875 det a,basis
R5067 T16875 T16843 dobj basis,provided
R5068 T16876 T16875 prep for,basis
R5069 T16877 T16876 pcomp distinguishing,for
R5070 T16878 T16879 det the,alleles
R5071 T16879 T16877 dobj alleles,distinguishing
R5072 T16880 T16881 amod wild,type
R5073 T16881 T16879 nmod type,alleles
R5074 T16882 T16881 punct -,type
R5075 T16883 T16881 cc and,type
R5076 T16884 T16881 conj mutant,type
R5077 T16885 T16877 prep by,distinguishing
R5078 T16886 T16887 compound Southern,blotting
R5079 T16887 T16885 pobj blotting,by
R5080 T16888 T16843 punct .,provided
R5081 T16890 T16891 det The,arm
R5082 T16891 T16896 nsubjpass arm,provided
R5083 T16892 T16891 amod long,arm
R5084 T16893 T16891 punct (,arm
R5085 T16894 T16891 amod downstream,arm
R5086 T16895 T16891 punct ),arm
R5087 T16897 T16891 prep of,arm
R5088 T16898 T16897 pobj homology,of
R5089 T16899 T16896 auxpass was,provided
R5090 T16900 T16896 agent by,provided
R5091 T16901 T16902 det a,fragment
R5092 T16902 T16900 pobj fragment,by
R5093 T16903 T16904 nummod 7,kb
R5094 T16904 T16902 compound kb,fragment
R5095 T16905 T16904 punct -,kb
R5096 T16906 T16907 compound HindIII,KpnI
R5097 T16907 T16902 compound KpnI,fragment
R5098 T16908 T16907 punct -,KpnI
R5099 T16909 T16902 punct ", ",fragment
R5100 T16910 T16902 acl extending,fragment
R5101 T16911 T16910 prep from,extending
R5102 T16912 T16911 pobj intron,from
R5103 T16913 T16912 nummod 7,intron
R5104 T16914 T16911 prep to,from
R5105 T16915 T16914 pobj intron,to
R5106 T16916 T16915 nummod 12,intron
R5107 T16917 T16902 punct ", ",fragment
R5108 T16918 T16902 acl inserted,fragment
R5109 T16919 T16918 prep between,inserted
R5110 T16920 T16921 det the,cassettes
R5111 T16921 T16919 pobj cassettes,between
R5112 T16922 T16923 nmod PGK,Neo
R5113 T16923 T16921 nmod Neo,cassettes
R5114 T16924 T16923 punct -,Neo
R5115 T16925 T16923 cc and,Neo
R5116 T16926 T16927 compound thymidine,kinase
R5117 T16927 T16923 conj kinase,Neo
R5118 T16928 T16927 punct (,kinase
R5119 T16929 T16927 appos tk,kinase
R5120 T16930 T16921 punct ),cassettes
R5121 T16931 T16896 punct .,provided
R5122 T17462 T17463 compound ES,culture
R5123 T17464 T17463 compound cell,culture
R5124 T17465 T17463 punct ", ",culture
R5125 T17466 T17463 conj transfection,culture
R5126 T17467 T17466 cc and,transfection
R5127 T17468 T17466 conj selection,transfection
R5128 T17469 T17468 prep of,selection
R5129 T17470 T17471 amod targeted,clones
R5130 T17471 T17469 pobj clones,of
R5131 T17473 T17474 compound Mouse,cells
R5132 T17474 T17477 nsubjpass cells,maintained
R5133 T17475 T17474 compound R1,cells
R5134 T17476 T17474 compound ES,cells
R5135 T17478 T17479 punct [,49
R5136 T17479 T17474 parataxis 49,cells
R5137 T17480 T17479 punct ],49
R5138 T17481 T17477 auxpass were,maintained
R5139 T17482 T17477 prep on,maintained
R5140 T17483 T17484 npadvmod gelatin,coated
R5141 T17484 T17486 amod coated,plates
R5142 T17485 T17484 punct -,coated
R5143 T17486 T17482 pobj plates,on
R5144 T17487 T17477 prep with,maintained
R5145 T17488 T17489 compound feeder,layers
R5146 T17489 T17487 pobj layers,with
R5147 T17490 T17489 prep of,layers
R5148 T17491 T17492 nmod mouse,fibroblasts
R5149 T17492 T17490 pobj fibroblasts,of
R5150 T17493 T17492 amod embryonic,fibroblasts
R5151 T17494 T17477 prep at,maintained
R5152 T17495 T17496 nummod 37,°C
R5153 T17496 T17494 pobj °C,at
R5154 T17497 T17477 prep under,maintained
R5155 T17498 T17499 nummod 5,%
R5156 T17499 T17500 compound %,CO2
R5157 T17500 T17497 pobj CO2,under
R5158 T17501 T17477 prep in,maintained
R5159 T17502 T17503 compound ES,medium
R5160 T17503 T17501 pobj medium,in
R5161 T17504 T17503 compound cell,medium
R5162 T17505 T17506 punct (,DMEM
R5163 T17506 T17477 parataxis DMEM,maintained
R5164 T17507 T17508 punct [,glucose
R5165 T17508 T17506 parataxis glucose,DMEM
R5166 T17509 T17508 amod high,glucose
R5167 T17510 T17506 punct ],DMEM
R5168 T17511 T17506 acl supplemented,DMEM
R5169 T17512 T17511 prep with,supplemented
R5170 T17513 T17514 nummod 15,%
R5171 T17514 T17515 nmod %,serum
R5172 T17515 T17512 pobj serum,with
R5173 T17516 T17515 amod fetal,serum
R5174 T17517 T17515 amod bovine,serum
R5175 T17518 T17515 punct ", ",serum
R5176 T17519 T17520 nummod 0.1,mM
R5177 T17520 T17521 nmod mM,acids
R5178 T17521 T17515 conj acids,serum
R5179 T17522 T17521 amod non-essential,acids
R5180 T17523 T17521 compound amino,acids
R5181 T17524 T17521 punct ", ",acids
R5182 T17525 T17526 nummod 2,mM
R5183 T17526 T17527 compound mM,glutamine
R5184 T17527 T17521 conj glutamine,acids
R5185 T17528 T17527 punct ", ",glutamine
R5186 T17529 T17530 nummod 1,mM
R5187 T17530 T17531 compound mM,pyruvate
R5188 T17531 T17527 conj pyruvate,glutamine
R5189 T17532 T17531 compound sodium,pyruvate
R5190 T17533 T17531 punct ", ",pyruvate
R5191 T17534 T17535 nummod 0.1,mM
R5192 T17535 T17536 nmod mM,mercaptoethanol
R5193 T17536 T17531 conj mercaptoethanol,pyruvate
R5194 T17537 T17536 nummod 2,mercaptoethanol
R5195 T17538 T17536 punct -,mercaptoethanol
R5196 T17539 T17536 punct ", ",mercaptoethanol
R5197 T17540 T17541 nummod 100,U
R5198 T17541 T17542 nmod U,penicillin
R5199 T17542 T17536 conj penicillin,mercaptoethanol
R5200 T17543 T17544 punct /,ml
R5201 T17544 T17541 prep ml,U
R5202 T17545 T17542 punct ", ",penicillin
R5203 T17546 T17547 nummod 100,μg
R5204 T17547 T17548 nmod μg,streptomycin
R5205 T17548 T17542 conj streptomycin,penicillin
R5206 T17549 T17550 punct /,ml
R5207 T17550 T17547 prep ml,μg
R5208 T17551 T17548 punct ", ",streptomycin
R5209 T17552 T17553 nummod 25,μg
R5210 T17553 T17554 nmod μg,amphotericin
R5211 T17554 T17548 conj amphotericin,streptomycin
R5212 T17555 T17556 punct /,ml
R5213 T17556 T17553 prep ml,μg
R5214 T17557 T17554 punct ", ",amphotericin
R5215 T17558 T17554 cc and,amphotericin
R5216 T17559 T17560 nummod "1,000",U
R5217 T17560 T17554 conj U,amphotericin
R5218 T17561 T17562 punct /,ml
R5219 T17562 T17560 prep ml,U
R5220 T17563 T17560 prep of,U
R5221 T17564 T17563 pobj ESGro,of
R5222 T17565 T17566 punct [,Chemicon
R5223 T17566 T17564 parataxis Chemicon,ESGro
R5224 T17567 T17566 punct ],Chemicon
R5225 T17568 T17477 punct ),maintained
R5226 T17569 T17477 punct .,maintained
R5227 T17571 T17572 amod Fetal,serum
R5228 T17572 T17574 nsubjpass serum,tested
R5229 T17573 T17572 amod bovine,serum
R5230 T17575 T17572 prep from,serum
R5231 T17576 T17577 compound HyClone,Inc
R5232 T17577 T17575 pobj Inc,from
R5233 T17578 T17577 compound Laboratories,Inc
R5234 T17579 T17580 punct (,Logan
R5235 T17580 T17577 parataxis Logan,Inc
R5236 T17581 T17580 punct ", ",Logan
R5237 T17582 T17580 npadvmod Utah,Logan
R5238 T17583 T17580 punct ),Logan
R5239 T17584 T17574 auxpass was,tested
R5240 T17585 T17574 prep for,tested
R5241 T17586 T17587 poss its,ability
R5242 T17587 T17585 pobj ability,for
R5243 T17588 T17589 aux to,support
R5244 T17589 T17587 acl support,ability
R5245 T17590 T17591 compound ES,cell
R5246 T17591 T17592 compound cell,growth
R5247 T17592 T17589 dobj growth,support
R5248 T17593 T17574 punct .,tested
R5249 T17595 T17596 nsubj Gelatin,was
R5250 T17597 T17596 prep from,was
R5251 T17598 T17599 compound Sigma,Aldrich
R5252 T17599 T17601 compound Aldrich,Canada
R5253 T17600 T17599 punct -,Aldrich
R5254 T17601 T17597 pobj Canada,from
R5255 T17602 T17603 punct (,Oakville
R5256 T17603 T17596 parataxis Oakville,was
R5257 T17604 T17603 punct ", ",Oakville
R5258 T17605 T17603 npadvmod Ontario,Oakville
R5259 T17606 T17603 punct ),Oakville
R5260 T17607 T17596 punct .,was
R5261 T17609 T17610 mark Unless,specified
R5262 T17610 T17612 advcl specified,were
R5263 T17611 T17610 advmod otherwise,specified
R5264 T17613 T17612 punct ", ",were
R5265 T17614 T17615 det all,reagents
R5266 T17615 T17612 nsubj reagents,were
R5267 T17616 T17615 amod other,reagents
R5268 T17617 T17618 compound tissue,culture
R5269 T17618 T17615 compound culture,reagents
R5270 T17619 T17612 prep from,were
R5271 T17620 T17621 compound Gibco,BRL
R5272 T17621 T17619 pobj BRL,from
R5273 T17622 T17621 punct -,BRL
R5274 T17623 T17612 punct .,were
R5275 T17625 T17626 det The,construct
R5276 T17626 T17628 nsubjpass construct,linearized
R5277 T17627 T17626 compound targeting,construct
R5278 T17629 T17628 auxpass was,linearized
R5279 T17630 T17628 agent by,linearized
R5280 T17631 T17632 compound NotI,digestion
R5281 T17632 T17630 pobj digestion,by
R5282 T17633 T17628 cc and,linearized
R5283 T17634 T17628 conj electroporated,linearized
R5284 T17635 T17634 prep into,electroporated
R5285 T17636 T17637 compound R1,cells
R5286 T17637 T17635 pobj cells,into
R5287 T17638 T17637 compound ES,cells
R5288 T17639 T17628 punct .,linearized
R5289 T17641 T17642 nsubjpass Cells,plated
R5290 T17643 T17642 auxpass were,plated
R5291 T17644 T17642 prep without,plated
R5292 T17645 T17646 compound feeder,layers
R5293 T17646 T17644 pobj layers,without
R5294 T17647 T17642 prep on,plated
R5295 T17648 T17649 npadvmod gelatin,coated
R5296 T17649 T17651 amod coated,plates
R5297 T17650 T17649 punct -,coated
R5298 T17651 T17647 pobj plates,on
R5299 T17652 T17642 cc and,plated
R5300 T17653 T17654 amod transformed,clones
R5301 T17654 T17655 nsubjpass clones,selected
R5302 T17655 T17642 conj selected,plated
R5303 T17656 T17655 auxpass were,selected
R5304 T17657 T17655 prep in,selected
R5305 T17658 T17659 det the,presence
R5306 T17659 T17657 pobj presence,in
R5307 T17660 T17659 prep of,presence
R5308 T17661 T17662 nummod 200,μg
R5309 T17662 T17663 nmod μg,G418
R5310 T17663 T17660 pobj G418,of
R5311 T17664 T17665 punct /,ml
R5312 T17665 T17662 prep ml,μg
R5313 T17666 T17667 punct (,BRL
R5314 T17667 T17663 parataxis BRL,G418
R5315 T17668 T17667 compound Gibco,BRL
R5316 T17669 T17667 punct -,BRL
R5317 T17670 T17667 punct ),BRL
R5318 T17671 T17663 cc and,G418
R5319 T17672 T17673 nummod 2,μM
R5320 T17673 T17674 compound μM,ganciclovir
R5321 T17674 T17663 conj ganciclovir,G418
R5322 T17675 T17676 punct (,Syntex
R5323 T17676 T17674 parataxis Syntex,ganciclovir
R5324 T17677 T17676 punct ", ",Syntex
R5325 T17678 T17676 amod Inc.,Syntex
R5326 T17679 T17676 punct ),Syntex
R5327 T17680 T17655 prep for,selected
R5328 T17681 T17682 nummod eight,days
R5329 T17682 T17680 pobj days,for
R5330 T17683 T17655 punct .,selected
R5331 T17685 T17686 npadvmod Drug,resistant
R5332 T17686 T17688 amod resistant,clones
R5333 T17687 T17686 punct -,resistant
R5334 T17688 T17689 nsubjpass clones,picked
R5335 T17690 T17689 auxpass were,picked
R5336 T17691 T17689 punct ", ",picked
R5337 T17692 T17689 conj expanded,picked
R5338 T17693 T17692 prep on,expanded
R5339 T17694 T17695 npadvmod gelatin,coated
R5340 T17695 T17697 amod coated,plates
R5341 T17696 T17695 punct -,coated
R5342 T17697 T17693 pobj plates,on
R5343 T17698 T17692 punct ", ",expanded
R5344 T17699 T17692 cc and,expanded
R5345 T17700 T17692 conj genotyped,expanded
R5346 T17701 T17700 prep by,genotyped
R5347 T17702 T17703 compound Southern,blotting
R5348 T17703 T17701 pobj blotting,by
R5349 T17704 T17703 cc and,blotting
R5350 T17705 T17706 compound PCR,analysis
R5351 T17706 T17703 conj analysis,blotting
R5352 T17707 T17708 punct (,see
R5353 T17708 T17700 parataxis see,genotyped
R5354 T17709 T17708 advmod below,see
R5355 T17710 T17708 punct ),see
R5356 T17711 T17689 punct .,picked
R5357 T17989 T17988 prep of,Generation
R5358 T17990 T17991 amod targeted,mice
R5359 T17991 T17989 pobj mice,of
R5360 T17993 T17994 nmod Capn2,cells
R5361 T17994 T17999 nsubjpass cells,aggregated
R5362 T17995 T17993 punct +,Capn2
R5363 T17996 T17993 punct /,Capn2
R5364 T17997 T17993 punct -,Capn2
R5365 T17998 T17994 compound ES,cells
R5366 T18000 T17999 auxpass were,aggregated
R5367 T18001 T17999 advmod overnight,aggregated
R5368 T18002 T17999 prep with,aggregated
R5369 T18003 T18004 nummod 8,cell
R5370 T18004 T18006 compound cell,embryos
R5371 T18005 T18004 punct -,cell
R5372 T18006 T18002 pobj embryos,with
R5373 T18007 T18006 acl recovered,embryos
R5374 T18008 T18007 prep from,recovered
R5375 T18009 T18010 compound CD1,matings
R5376 T18010 T18008 pobj matings,from
R5377 T18011 T17999 punct ", ",aggregated
R5378 T18012 T18013 mark as,described
R5379 T18013 T17999 advcl described,aggregated
R5380 T18014 T18013 advmod previously,described
R5381 T18015 T18016 punct [,31
R5382 T18016 T17999 parataxis 31,aggregated
R5383 T18017 T18016 punct ],31
R5384 T18018 T17999 punct .,aggregated
R5385 T18020 T18021 prep On,transferred
R5386 T18022 T18023 det the,day
R5387 T18023 T18020 pobj day,On
R5388 T18024 T18023 amod next,day
R5389 T18025 T18021 punct ", ",transferred
R5390 T18026 T18021 nsubjpass blastocysts,transferred
R5391 T18027 T18021 auxpass were,transferred
R5392 T18028 T18021 prep to,transferred
R5393 T18029 T18030 amod pseudopregnant,females
R5394 T18030 T18028 pobj females,to
R5395 T18031 T18030 compound CD1,females
R5396 T18032 T18021 punct .,transferred
R5397 T18034 T18035 amod Chimeric,animals
R5398 T18035 T18036 nsubj animals,were
R5399 T18037 T18036 acomp identifiable,were
R5400 T18038 T18036 prep at,were
R5401 T18039 T18038 pobj birth,at
R5402 T18040 T18036 prep by,were
R5403 T18041 T18042 amod black,pigmentation
R5404 T18042 T18040 pobj pigmentation,by
R5405 T18043 T18042 compound eye,pigmentation
R5406 T18044 T18040 cc and,by
R5407 T18045 T18046 advmod subsequently,by
R5408 T18046 T18040 conj by,by
R5409 T18047 T18046 pobj patches,by
R5410 T18048 T18047 prep of,patches
R5411 T18049 T18050 compound agouti,colour
R5412 T18050 T18048 pobj colour,of
R5413 T18051 T18050 compound coat,colour
R5414 T18052 T18036 punct .,were
R5415 T18054 T18055 amod Chimeric,males
R5416 T18055 T18056 nsubjpass males,bred
R5417 T18057 T18056 auxpass were,bred
R5418 T18058 T18056 prep with,bred
R5419 T18059 T18060 compound CD1,females
R5420 T18060 T18058 pobj females,with
R5421 T18061 T18062 aux to,identify
R5422 T18062 T18056 advcl identify,bred
R5423 T18063 T18064 det those,males
R5424 T18064 T18062 dobj males,identify
R5425 T18065 T18064 amod capable,males
R5426 T18066 T18065 prep of,capable
R5427 T18067 T18068 compound germline,transmission
R5428 T18068 T18066 pobj transmission,of
R5429 T18069 T18056 punct .,bred
R5430 T18071 T18072 nsubjpass These,bred
R5431 T18073 T18072 auxpass were,bred
R5432 T18074 T18072 advmod then,bred
R5433 T18075 T18072 prep with,bred
R5434 T18076 T18077 compound 129SvJ,females
R5435 T18077 T18075 pobj females,with
R5436 T18078 T18079 aux to,establish
R5437 T18079 T18072 advcl establish,bred
R5438 T18080 T18081 det the,mutation
R5439 T18081 T18079 dobj mutation,establish
R5440 T18082 T18079 prep in,establish
R5441 T18083 T18084 det an,background
R5442 T18084 T18082 pobj background,in
R5443 T18085 T18084 amod inbred,background
R5444 T18086 T18084 amod genetic,background
R5445 T18087 T18072 punct .,bred
R5446 T18089 T18090 compound Mouse,protocols
R5447 T18090 T18091 nsubjpass protocols,approved
R5448 T18092 T18091 auxpass were,approved
R5449 T18093 T18091 agent by,approved
R5450 T18094 T18095 det the,Queen
R5451 T18095 T18096 poss Queen,University
R5452 T18096 T18098 compound University,Committee
R5453 T18097 T18095 case 's,Queen
R5454 T18098 T18093 pobj Committee,by
R5455 T18099 T18100 compound Animal,Care
R5456 T18100 T18098 compound Care,Committee
R5457 T18101 T18091 prep according,approved
R5458 T18102 T18101 prep to,according
R5459 T18103 T18104 det the,guidelines
R5460 T18104 T18102 pobj guidelines,to
R5461 T18105 T18104 prep of,guidelines
R5462 T18106 T18107 det the,Council
R5463 T18107 T18105 pobj Council,of
R5464 T18108 T18107 compound Canadian,Council
R5465 T18109 T18107 prep on,Council
R5466 T18110 T18111 compound Animal,Care
R5467 T18111 T18109 pobj Care,on
R5468 T18112 T18091 punct .,approved
R5469 T19530 T19531 compound Genotyping,methods
R5470 T19533 T19534 amod Several,blot
R5471 T19534 T19536 nsubjpass blot,exploited
R5472 T19535 T19534 compound Southern,blot
R5473 T19537 T19534 cc and,blot
R5474 T19538 T19539 compound PCR,strategies
R5475 T19539 T19534 conj strategies,blot
R5476 T19540 T19536 auxpass were,exploited
R5477 T19541 T19536 prep in,exploited
R5478 T19542 T19541 pobj order,in
R5479 T19543 T19544 aux to,determine
R5480 T19544 T19542 acl determine,order
R5481 T19545 T19546 det the,genotype
R5482 T19546 T19544 dobj genotype,determine
R5483 T19547 T19546 prep of,genotype
R5484 T19548 T19549 det the,locus
R5485 T19549 T19547 pobj locus,of
R5486 T19550 T19549 compound Capn2,locus
R5487 T19551 T19536 punct .,exploited
R5488 T19553 T19554 compound Southern,blotting
R5489 T19554 T19555 nsubjpass blotting,carried
R5490 T19556 T19555 auxpass was,carried
R5491 T19557 T19555 prt out,carried
R5492 T19558 T19555 advcl using,carried
R5493 T19559 T19560 det the,system
R5494 T19560 T19558 dobj system,using
R5495 T19561 T19560 nmod digoxigenin,system
R5496 T19562 T19561 punct (,digoxigenin
R5497 T19563 T19561 appos DIG,digoxigenin
R5498 T19564 T19560 punct ),system
R5499 T19565 T19560 amod non-radioactive,system
R5500 T19566 T19567 punct (,Roche
R5501 T19567 T19560 parataxis Roche,system
R5502 T19568 T19567 punct ),Roche
R5503 T19569 T19555 punct .,carried
R5504 T19571 T19572 prep In,blotted
R5505 T19573 T19574 amod most,cases
R5506 T19574 T19571 pobj cases,In
R5507 T19575 T19572 punct ", ",blotted
R5508 T19576 T19572 nsubjpass membranes,blotted
R5509 T19577 T19572 auxpass were,blotted
R5510 T19578 T19572 prep with,blotted
R5511 T19579 T19580 npadvmod BamHI,digested
R5512 T19580 T19582 amod digested,DNA
R5513 T19581 T19580 punct -,digested
R5514 T19582 T19578 pobj DNA,with
R5515 T19583 T19582 amod genomic,DNA
R5516 T19584 T19572 cc and,blotted
R5517 T19585 T19572 conj hybridized,blotted
R5518 T19586 T19585 prep with,hybridized
R5519 T19587 T19588 det a,fragment
R5520 T19588 T19586 pobj fragment,with
R5521 T19589 T19590 npadvmod DIG,labeled
R5522 T19590 T19588 amod labeled,fragment
R5523 T19591 T19590 punct -,labeled
R5524 T19592 T19593 nummod 823,bp
R5525 T19593 T19588 nmod bp,fragment
R5526 T19594 T19595 npadvmod exon,containing
R5527 T19595 T19588 amod containing,fragment
R5528 T19596 T19594 nummod 4,exon
R5529 T19597 T19595 punct -,containing
R5530 T19598 T19599 compound BamHI,HindIII
R5531 T19599 T19588 compound HindIII,fragment
R5532 T19600 T19599 punct -,HindIII
R5533 T19601 T19588 acl located,fragment
R5534 T19602 T19603 advmod immediately,upstream
R5535 T19603 T19601 advmod upstream,located
R5536 T19604 T19603 prep of,upstream
R5537 T19605 T19606 det the,arm
R5538 T19606 T19604 pobj arm,of
R5539 T19607 T19606 amod short,arm
R5540 T19608 T19606 prep of,arm
R5541 T19609 T19608 pobj homology,of
R5542 T19610 T19611 punct (,Figure
R5543 T19611 T19585 parataxis Figure,hybridized
R5544 T19612 T19611 nummod 1,Figure
R5545 T19613 T19611 punct ),Figure
R5546 T19614 T19572 punct .,blotted
R5547 T19616 T19617 det A,fragment
R5548 T19617 T19623 nsubjpass fragment,used
R5549 T19618 T19619 nummod 681,bp
R5550 T19619 T19617 compound bp,fragment
R5551 T19620 T19621 compound PstI,XbaI
R5552 T19621 T19617 compound XbaI,fragment
R5553 T19622 T19621 punct -,XbaI
R5554 T19624 T19617 prep from,fragment
R5555 T19625 T19626 det the,cassette
R5556 T19626 T19624 pobj cassette,from
R5557 T19627 T19628 compound PGK,Neo
R5558 T19628 T19626 compound Neo,cassette
R5559 T19629 T19628 punct -,Neo
R5560 T19630 T19623 auxpass was,used
R5561 T19631 T19623 advmod also,used
R5562 T19632 T19633 aux to,probe
R5563 T19633 T19623 advcl probe,used
R5564 T19634 T19635 compound Southern,blots
R5565 T19635 T19633 dobj blots,probe
R5566 T19636 T19623 prep in,used
R5567 T19637 T19636 pobj order,in
R5568 T19638 T19639 aux to,verify
R5569 T19639 T19637 acl verify,order
R5570 T19640 T19641 det a,event
R5571 T19641 T19639 dobj event,verify
R5572 T19642 T19641 amod single,event
R5573 T19643 T19641 compound integration,event
R5574 T19644 T19623 prep in,used
R5575 T19645 T19646 amod targeted,clones
R5576 T19646 T19644 pobj clones,in
R5577 T19647 T19623 punct .,used
R5578 T19649 T19650 nsubjpass Genotyping,carried
R5579 T19651 T19650 auxpass was,carried
R5580 T19652 T19650 advmod also,carried
R5581 T19653 T19650 prt out,carried
R5582 T19654 T19650 agent by,carried
R5583 T19655 T19656 compound PCR,analysis
R5584 T19656 T19654 pobj analysis,by
R5585 T19657 T19656 prep of,analysis
R5586 T19658 T19659 amod genomic,DNA
R5587 T19659 T19657 pobj DNA,of
R5588 T19660 T19650 punct .,carried
R5589 T19662 T19663 det The,sequences
R5590 T19663 T19664 nsubjpass sequences,listed
R5591 T19665 T19663 prep of,sequences
R5592 T19666 T19667 det all,primers
R5593 T19667 T19665 pobj primers,of
R5594 T19668 T19667 compound oligonucleotide,primers
R5595 T19669 T19664 auxpass are,listed
R5596 T19670 T19664 prep in,listed
R5597 T19671 T19670 pobj Table,in
R5598 T19672 T19671 nummod 1,Table
R5599 T19673 T19664 punct .,listed
R5600 T19675 T19676 det A,strategy
R5601 T19676 T19681 nsubj strategy,was
R5602 T19677 T19678 amod single,step
R5603 T19678 T19676 compound step,strategy
R5604 T19679 T19678 punct -,step
R5605 T19680 T19676 compound PCR,strategy
R5606 T19682 T19681 acomp sufficient,was
R5607 T19683 T19682 prep for,sufficient
R5608 T19684 T19683 pcomp genotyping,for
R5609 T19685 T19686 compound ES,cells
R5610 T19686 T19684 dobj cells,genotyping
R5611 T19687 T19686 cc or,cells
R5612 T19688 T19686 conj biopsies,cells
R5613 T19689 T19688 prep from,biopsies
R5614 T19690 T19691 amod post-implantation,embryos
R5615 T19691 T19689 pobj embryos,from
R5616 T19692 T19691 cc and,embryos
R5617 T19693 T19691 conj weanlings,embryos
R5618 T19694 T19695 punct (,Figure
R5619 T19695 T19684 parataxis Figure,genotyping
R5620 T19696 T19695 nummod 1,Figure
R5621 T19697 T19695 punct ),Figure
R5622 T19698 T19681 punct .,was
R5623 T19700 T19701 det A,segment
R5624 T19701 T19704 nsubjpass segment,amplified
R5625 T19702 T19703 nummod "2,748",bp
R5626 T19703 T19701 compound bp,segment
R5627 T19705 T19701 prep of,segment
R5628 T19706 T19707 det the,allele
R5629 T19707 T19705 pobj allele,of
R5630 T19708 T19709 amod wild,type
R5631 T19709 T19707 compound type,allele
R5632 T19710 T19709 punct -,type
R5633 T19711 T19701 cc and,segment
R5634 T19712 T19713 det a,segment
R5635 T19713 T19701 conj segment,segment
R5636 T19714 T19715 nummod "2,711",bp
R5637 T19715 T19713 compound bp,segment
R5638 T19716 T19713 prep of,segment
R5639 T19717 T19718 det the,allele
R5640 T19718 T19716 pobj allele,of
R5641 T19719 T19718 compound mutant,allele
R5642 T19720 T19704 auxpass were,amplified
R5643 T19721 T19704 prep in,amplified
R5644 T19722 T19723 amod separate,reactions
R5645 T19723 T19721 pobj reactions,in
R5646 T19724 T19704 advcl using,amplified
R5647 T19725 T19726 det a,primer
R5648 T19726 T19724 dobj primer,using
R5649 T19727 T19728 amod common,sense
R5650 T19728 T19726 compound sense,primer
R5651 T19729 T19730 punct (,intron
R5652 T19730 T19728 parataxis intron,sense
R5653 T19731 T19730 nummod 4,intron
R5654 T19732 T19730 punct ),intron
R5655 T19733 T19726 punct ", ",primer
R5656 T19734 T19726 acl located,primer
R5657 T19735 T19734 prep outside,located
R5658 T19736 T19737 det the,arm
R5659 T19737 T19735 pobj arm,outside
R5660 T19738 T19737 amod short,arm
R5661 T19739 T19737 prep of,arm
R5662 T19740 T19739 pobj homology,of
R5663 T19741 T19726 punct ", ",primer
R5664 T19742 T19726 cc and,primer
R5665 T19743 T19744 amod distinct,primers
R5666 T19744 T19726 conj primers,primer
R5667 T19745 T19744 compound antisense,primers
R5668 T19746 T19747 dep which,hybridized
R5669 T19747 T19744 relcl hybridized,primers
R5670 T19748 T19747 prep to,hybridized
R5671 T19749 T19750 preconj either,type
R5672 T19750 T19753 nmod type,sequence
R5673 T19751 T19750 amod wild,type
R5674 T19752 T19750 punct -,type
R5675 T19753 T19748 pobj sequence,to
R5676 T19754 T19755 punct (,exon
R5677 T19755 T19750 parataxis exon,type
R5678 T19756 T19755 nummod 7,exon
R5679 T19757 T19755 punct ),exon
R5680 T19758 T19750 cc or,type
R5681 T19759 T19750 conj mutant,type
R5682 T19760 T19761 punct (,Neo
R5683 T19761 T19759 parataxis Neo,mutant
R5684 T19762 T19761 compound PGK,Neo
R5685 T19763 T19761 punct -,Neo
R5686 T19764 T19761 punct ),Neo
R5687 T19765 T19766 punct (,I
R5688 T19766 T19747 parataxis I,hybridized
R5689 T19767 T19766 compound Table,I
R5690 T19768 T19766 punct ),I
R5691 T19769 T19704 punct .,amplified
R5692 T19771 T19772 det The,parameters
R5693 T19772 T19774 nsubj parameters,included
R5694 T19773 T19772 amod thermocycling,parameters
R5695 T19775 T19776 det a,step
R5696 T19776 T19774 dobj step,included
R5697 T19777 T19778 nummod five,minute
R5698 T19778 T19776 nmod minute,step
R5699 T19779 T19776 amod initial,step
R5700 T19780 T19776 compound denaturation,step
R5701 T19781 T19776 prep at,step
R5702 T19782 T19783 nummod 95,°C
R5703 T19783 T19781 pobj °C,at
R5704 T19784 T19776 punct ", ",step
R5705 T19785 T19786 nummod 30,cycles
R5706 T19786 T19776 conj cycles,step
R5707 T19787 T19786 prep of,cycles
R5716 T19796 T19786 conj minute,cycles
R5717 T19797 T19796 amod annealing,minute
R5718 T19798 T19796 prep at,minute
R5719 T19799 T19800 nummod 56,°C
R5720 T19800 T19798 pobj °C,at
R5721 T19801 T19796 punct ", ",minute
R5722 T19802 T19796 cc and,minute
R5723 T19803 T19804 nummod one,minute
R5724 T19804 T19805 compound minute,extension
R5725 T19805 T19796 conj extension,minute
R5726 T19806 T19805 prep at,extension
R5727 T19807 T19808 nummod 72,°C
R5728 T19808 T19806 pobj °C,at
R5729 T19809 T19776 punct ", ",step
R5730 T19810 T19776 prep with,step
R5731 T19811 T19812 det a,step
R5732 T19812 T19810 pobj step,with
R5733 T19813 T19814 nummod ten,minute
R5734 T19814 T19812 nmod minute,step
R5735 T19815 T19812 amod final,step
R5736 T19816 T19812 compound extension,step
R5737 T19817 T19774 punct .,included
R5738 T19820 T19821 prep Due,developed
R5739 T19822 T19820 pcomp to,Due
R5740 T19823 T19824 det the,amount
R5741 T19824 T19820 pobj amount,Due
R5742 T19825 T19824 amod limited,amount
R5743 T19826 T19824 prep of,amount
R5744 T19827 T19828 amod genetic,material
R5745 T19828 T19826 pobj material,of
R5746 T19829 T19828 amod available,material
R5747 T19830 T19824 prep in,amount
R5748 T19831 T19832 amod pre-implantation,embryos
R5749 T19832 T19830 pobj embryos,in
R5750 T19833 T19821 punct ", ",developed
R5751 T19834 T19835 det a,strategy
R5752 T19835 T19821 nsubjpass strategy,developed
R5753 T19836 T19835 amod nested,strategy
R5754 T19837 T19835 compound PCR,strategy
R5755 T19838 T19821 auxpass was,developed
R5756 T19839 T19840 aux to,yield
R5757 T19840 T19821 advcl yield,developed
R5758 T19841 T19842 amod reliable,information
R5759 T19842 T19840 dobj information,yield
R5760 T19843 T19842 compound genotyping,information
R5761 T19844 T19845 punct (,Figure
R5762 T19845 T19821 parataxis Figure,developed
R5763 T19846 T19845 nummod 4,Figure
R5764 T19847 T19845 punct ),Figure
R5765 T19848 T19821 punct .,developed
R5766 T19850 T19851 amod Whole,embryos
R5767 T19851 T19852 nsubjpass embryos,digested
R5768 T19853 T19852 auxpass were,digested
R5769 T19854 T19852 advmod first,digested
R5770 T19855 T19852 prep in,digested
R5771 T19856 T19857 nummod 20,μL
R5772 T19857 T19855 pobj μL,in
R5773 T19858 T19857 prep of,μL
R5774 T19859 T19860 compound proteinase,K
R5775 T19860 T19861 compound K,buffer
R5776 T19861 T19858 pobj buffer,of
R5777 T19862 T19863 punct (,see
R5778 T19863 T19852 parataxis see,digested
R5779 T19864 T19863 advmod below,see
R5780 T19865 T19863 punct ),see
R5781 T19866 T19852 punct .,digested
R5782 T19868 T19869 det The,lysate
R5783 T19869 T19870 nsubjpass lysate,divided
R5784 T19871 T19870 auxpass was,divided
R5785 T19872 T19870 advmod then,divided
R5786 T19873 T19870 prep in,divided
R5787 T19874 T19873 pobj two,in
R5788 T19875 T19870 punct ", ",divided
R5789 T19876 T19870 prep with,divided
R5790 T19877 T19878 nsubjpass half,used
R5791 T19878 T19876 pcomp used,with
R5792 T19879 T19880 punct (,μL
R5793 T19880 T19877 parataxis μL,half
R5794 T19881 T19880 nummod 10,μL
R5795 T19882 T19880 punct ),μL
R5796 T19883 T19878 auxpass being,used
R5797 T19884 T19878 prep in,used
R5798 T19885 T19886 det the,amplification
R5799 T19886 T19884 pobj amplification,in
R5800 T19887 T19886 prep of,amplification
R5801 T19888 T19889 det the,allele
R5802 T19889 T19887 pobj allele,of
R5803 T19890 T19891 amod wild,type
R5804 T19891 T19889 compound type,allele
R5805 T19892 T19891 punct -,type
R5806 T19893 T19878 cc and,used
R5807 T19894 T19895 det the,μL
R5808 T19895 T19878 conj μL,used
R5809 T19896 T19895 amod remaining,μL
R5810 T19897 T19895 nummod 10,μL
R5811 T19898 T19895 prep in,μL
R5812 T19899 T19900 det the,amplification
R5813 T19900 T19898 pobj amplification,in
R5814 T19901 T19900 prep of,amplification
R5815 T19902 T19903 det the,allele
R5816 T19903 T19901 pobj allele,of
R5817 T19904 T19903 compound mutant,allele
R5818 T19905 T19870 punct .,divided
R5819 T19907 T19908 det The,reaction
R5820 T19908 T19910 nsubjpass reaction,carried
R5821 T19909 T19908 amod first,reaction
R5822 T19911 T19908 prep in,reaction
R5823 T19912 T19913 det the,amplification
R5824 T19913 T19911 pobj amplification,in
R5825 T19914 T19913 amod nested,amplification
R5826 T19915 T19913 compound PCR,amplification
R5827 T19916 T19913 prep of,amplification
R5828 T19917 T19918 det the,allele
R5829 T19918 T19916 pobj allele,of
R5830 T19919 T19920 amod wild,type
R5831 T19920 T19918 compound type,allele
R5832 T19921 T19920 punct -,type
R5833 T19922 T19910 auxpass was,carried
R5834 T19923 T19910 prt out,carried
R5835 T19924 T19910 prep in,carried
R5836 T19925 T19926 det a,volume
R5837 T19926 T19924 pobj volume,in
R5838 T19927 T19926 amod final,volume
R5839 T19928 T19926 compound reaction,volume
R5840 T19929 T19926 prep of,volume
R5841 T19930 T19931 nummod 50,μL
R5842 T19931 T19929 pobj μL,of
R5843 T19932 T19910 punct ", ",carried
R5844 T19933 T19910 advcl using,carried
R5845 T19934 T19935 det an,primer
R5846 T19935 T19933 dobj primer,using
R5847 T19936 T19935 nmod intron,primer
R5848 T19937 T19936 nummod 6,intron
R5849 T19938 T19935 compound sense,primer
R5850 T19939 T19935 cc and,primer
R5851 T19940 T19941 det an,primer
R5852 T19941 T19935 conj primer,primer
R5853 T19942 T19941 compound antisense,primer
R5854 T19943 T19941 acl located,primer
R5855 T19944 T19943 prep in,located
R5856 T19945 T19944 pobj intron,in
R5857 T19946 T19945 nummod 7,intron
R5858 T19947 T19910 punct .,carried
R5859 T19949 T19950 nummod Two,μL
R5860 T19950 T19951 nsubjpass μL,used
R5861 T19952 T19950 prep of,μL
R5862 T19953 T19954 det the,reaction
R5863 T19954 T19952 pobj reaction,of
R5864 T19955 T19954 amod first,reaction
R5865 T19956 T19951 auxpass were,used
R5866 T19957 T19951 prep as,used
R5867 T19958 T19957 pobj template,as
R5868 T19959 T19951 prep in,used
R5869 T19960 T19961 det the,amplification
R5870 T19961 T19959 pobj amplification,in
R5871 T19962 T19961 amod second,amplification
R5872 T19963 T19961 compound PCR,amplification
R5873 T19964 T19951 advcl using,used
R5874 T19965 T19966 det another,primer
R5875 T19966 T19964 dobj primer,using
R5876 T19967 T19966 nmod intron,primer
R5877 T19968 T19967 nummod 6,intron
R5878 T19969 T19966 compound sense,primer
R5879 T19970 T19966 cc and,primer
R5880 T19971 T19972 det an,primer
R5881 T19972 T19966 conj primer,primer
R5882 T19973 T19972 compound antisense,primer
R5883 T19974 T19972 prep in,primer
R5884 T19975 T19974 pobj exon,in
R5885 T19976 T19975 nummod 7,exon
R5886 T19977 T19951 punct .,used
R5887 T19979 T19980 det The,amplification
R5888 T19980 T19982 nsubjpass amplification,carried
R5889 T19981 T19980 amod nested,amplification
R5890 T19983 T19980 prep of,amplification
R5891 T19984 T19985 det the,allele
R5892 T19985 T19983 pobj allele,of
R5893 T19986 T19985 compound mutant,allele
R5894 T19987 T19982 auxpass was,carried
R5895 T19988 T19982 prt out,carried
R5896 T19989 T19982 advmod similarly,carried
R5897 T19990 T19982 punct .,carried
R5898 T19992 T19993 det The,pair
R5899 T19993 T19996 nsubj pair,consisted
R5900 T19994 T19993 amod first,pair
R5901 T19995 T19993 compound primer,pair
R5902 T19997 T19996 prep of,consisted
R5903 T19998 T19999 det the,primer
R5904 T19999 T19997 pobj primer,of
R5905 T20000 T19999 nmod intron,primer
R5906 T20001 T20000 nummod 6,intron
R5907 T20002 T19999 compound sense,primer
R5908 T20003 T19999 cc and,primer
R5909 T20004 T20005 det an,primer
R5910 T20005 T19999 conj primer,primer
R5911 T20006 T20005 compound antisense,primer
R5912 T20007 T20005 prep in,primer
R5913 T20008 T20009 det the,cassette
R5914 T20009 T20007 pobj cassette,in
R5915 T20010 T20011 compound PGK,Neo
R5916 T20011 T20009 compound Neo,cassette
R5917 T20012 T20011 punct -,Neo
R5918 T20013 T19996 punct .,consisted
R5919 T20015 T20016 det The,pair
R5920 T20016 T20019 nsubjpass pair,comprised
R5921 T20017 T20016 amod nested,pair
R5922 T20018 T20016 compound primer,pair
R5923 T20020 T20019 auxpass was,comprised
R5924 T20021 T20019 prep of,comprised
R5925 T20022 T20023 det the,primer
R5926 T20023 T20021 pobj primer,of
R5927 T20024 T20023 amod second,primer
R5928 T20025 T20023 nmod intron,primer
R5929 T20026 T20025 nummod 6,intron
R5930 T20027 T20023 compound sense,primer
R5931 T20028 T20023 cc and,primer
R5932 T20029 T20030 det an,primer
R5933 T20030 T20023 conj primer,primer
R5934 T20031 T20030 compound antisense,primer
R5935 T20032 T20033 advmod also,located
R5936 T20033 T20030 acl located,primer
R5937 T20034 T20033 prep within,located
R5938 T20035 T20036 det the,sequence
R5939 T20036 T20034 pobj sequence,within
R5940 T20037 T20038 compound PGK,Neo
R5941 T20038 T20036 compound Neo,sequence
R5942 T20039 T20038 punct -,Neo
R5943 T20040 T20019 punct .,comprised
R5944 T20042 T20043 nsubjpass It,noted
R5945 T20044 T20043 aux should,noted
R5946 T20045 T20043 auxpass be,noted
R5947 T20046 T20047 mark that,involved
R5948 T20047 T20043 ccomp involved,noted
R5949 T20048 T20047 nsubj amplification,involved
R5950 T20049 T20048 prep of,amplification
R5951 T20050 T20051 det both,sequences
R5952 T20051 T20049 pobj sequences,of
R5953 T20052 T20053 det the,primers
R5954 T20053 T20047 dobj primers,involved
R5955 T20054 T20053 amod same,primers
R5956 T20055 T20053 compound sense,primers
R5957 T20056 T20047 prep in,involved
R5958 T20057 T20058 det both,steps
R5959 T20058 T20056 pobj steps,in
R5960 T20059 T20058 prep of,steps
R5961 T20060 T20061 det the,strategy
R5962 T20061 T20059 pobj strategy,of
R5963 T20062 T20061 amod nested,strategy
R5964 T20063 T20061 compound PCR,strategy
R5965 T20064 T20043 punct .,noted
R5966 T20066 T20067 prep In,included
R5967 T20068 T20066 pobj addition,In
R5968 T20069 T20067 punct ", ",included
R5969 T20070 T20071 det the,sets
R5970 T20071 T20067 nsubj sets,included
R5971 T20072 T20071 nummod two,sets
R5972 T20073 T20071 prep of,sets
R5973 T20074 T20073 pobj reactions,of
R5974 T20075 T20076 det a,control
R5975 T20076 T20067 dobj control,included
R5976 T20077 T20076 amod common,control
R5977 T20078 T20076 amod internal,control
R5978 T20079 T20076 acl designed,control
R5979 T20080 T20081 aux to,amplify
R5980 T20081 T20079 advcl amplify,designed
R5981 T20082 T20083 det a,region
R5982 T20083 T20081 dobj region,amplify
R5983 T20084 T20083 amod genomic,region
R5984 T20085 T20083 prep within,region
R5985 T20086 T20087 det the,arm
R5986 T20087 T20085 pobj arm,within
R5987 T20088 T20087 amod short,arm
R5988 T20089 T20087 prep of,arm
R5989 T20090 T20091 det the,vector
R5990 T20091 T20089 pobj vector,of
R5991 T20092 T20091 compound targeting,vector
R5992 T20093 T20094 dep that,preserved
R5993 T20094 T20083 relcl preserved,region
R5994 T20095 T20094 auxpass is,preserved
R5995 T20096 T20094 prep in,preserved
R5996 T20097 T20098 preconj both,alleles
R5997 T20098 T20096 pobj alleles,in
R5998 T20099 T20098 det the,alleles
R5999 T20100 T20101 amod wild,type
R6000 T20101 T20098 nmod type,alleles
R6001 T20102 T20101 punct -,type
R6002 T20103 T20101 cc and,type
R6003 T20104 T20101 conj mutant,type
R6004 T20105 T20067 punct .,included
R6005 T20107 T20108 det The,pair
R6006 T20108 T20111 nsubjpass pair,made
R6007 T20109 T20108 amod first,pair
R6008 T20110 T20108 compound primer,pair
R6009 T20112 T20108 prep of,pair
R6010 T20113 T20114 det the,PCR
R6011 T20114 T20112 pobj PCR,of
R6012 T20115 T20114 compound control,PCR
R6013 T20116 T20111 auxpass was,made
R6014 T20117 T20111 prt up,made
R6015 T20118 T20111 prep of,made
R6016 T20119 T20120 det an,primer
R6017 T20120 T20118 pobj primer,of
R6018 T20121 T20120 nmod intron,primer
R6019 T20122 T20121 nummod 4,intron
R6020 T20123 T20120 compound sense,primer
R6021 T20124 T20120 cc and,primer
R6022 T20125 T20126 det an,primer
R6023 T20126 T20120 conj primer,primer
R6024 T20127 T20126 nmod intron,primer
R6025 T20128 T20127 nummod 5,intron
R6026 T20129 T20126 compound antisense,primer
R6027 T20130 T20111 punct .,made
R6028 T20132 T20133 det The,pair
R6029 T20133 T20136 nsubj pair,comprised
R6030 T20134 T20133 amod second,pair
R6031 T20135 T20133 compound primer,pair
R6032 T20137 T20138 det an,primer
R6033 T20138 T20136 dobj primer,comprised
R6034 T20139 T20138 nmod exon,primer
R6035 T20140 T20139 nummod 5,exon
R6036 T20141 T20138 compound sense,primer
R6037 T20142 T20138 cc and,primer
R6038 T20143 T20144 det a,primer
R6039 T20144 T20138 conj primer,primer
R6040 T20145 T20144 amod nested,primer
R6041 T20146 T20144 nmod intron,primer
R6042 T20147 T20146 nummod 5,intron
R6043 T20148 T20144 compound antisense,primer
R6044 T20149 T20136 punct .,comprised
R6045 T20151 T20152 det The,products
R6046 T20152 T20154 nsubj products,were
R6047 T20153 T20152 amod final,products
R6048 T20155 T20156 nummod 213,bp
R6049 T20156 T20154 attr bp,were
R6050 T20157 T20154 prep for,were
R6051 T20158 T20159 det the,PCR
R6052 T20159 T20157 pobj PCR,for
R6053 T20160 T20159 compound control,PCR
R6054 T20161 T20154 punct ", ",were
R6055 T20162 T20163 nummod 429,bp
R6056 T20163 T20154 conj bp,were
R6057 T20164 T20163 prep for,bp
R6058 T20165 T20166 det the,PCR
R6059 T20166 T20164 pobj PCR,for
R6060 T20167 T20168 amod wild,type
R6061 T20168 T20166 compound type,PCR
R6062 T20169 T20168 punct -,type
R6063 T20170 T20163 punct ", ",bp
R6064 T20171 T20163 cc and,bp
R6065 T20172 T20173 nummod 389,bp
R6066 T20173 T20163 conj bp,bp
R6067 T20174 T20173 prep for,bp
R6068 T20175 T20176 det the,PCR
R6069 T20176 T20174 pobj PCR,for
R6070 T20177 T20176 compound mutant,PCR
R6071 T20178 T20154 punct .,were
R6072 T20180 T20181 det All,reactions
R6073 T20181 T20182 nsubjpass reactions,carried
R6074 T20183 T20182 auxpass were,carried
R6075 T20184 T20182 prt out,carried
R6076 T20185 T20182 advcl using,carried
R6077 T20186 T20187 amod identical,conditions
R6078 T20187 T20185 dobj conditions,using
R6079 T20188 T20187 compound PCR,conditions
R6080 T20189 T20187 acl entailing,conditions
R6081 T20190 T20191 det an,denaturation
R6082 T20191 T20189 dobj denaturation,entailing
R6083 T20192 T20191 amod initial,denaturation
R6084 T20193 T20194 nummod five,minute
R6085 T20194 T20191 compound minute,denaturation
R6086 T20195 T20191 prep at,denaturation
R6087 T20196 T20197 nummod 95,°C
R6088 T20197 T20195 pobj °C,at
R6089 T20198 T20191 punct ", ",denaturation
R6090 T20199 T20200 nummod 35,cycles
R6091 T20200 T20191 conj cycles,denaturation
R6092 T20201 T20200 prep of,cycles
R6093 T20202 T20203 nummod one,denaturation
R6094 T20203 T20201 pobj denaturation,of
R6095 T20204 T20203 compound minute,denaturation
R6096 T20205 T20200 prep at,cycles
R6097 T20206 T20207 nummod 95,°C
R6098 T20207 T20205 pobj °C,at
R6099 T20208 T20200 punct ", ",cycles
R6100 T20209 T20210 nummod one,minute
R6101 T20210 T20200 conj minute,cycles
R6102 T20211 T20210 amod annealing,minute
R6103 T20212 T20210 prep at,minute
R6104 T20213 T20214 nummod 56,°C
R6105 T20214 T20212 pobj °C,at
R6106 T20215 T20210 punct ", ",minute
R6107 T20216 T20210 cc and,minute
R6108 T20217 T20218 nummod one,minute
R6109 T20218 T20219 compound minute,extension
R6110 T20219 T20210 conj extension,minute
R6111 T20220 T20219 prep at,extension
R6112 T20221 T20222 nummod 72,°C
R6113 T20222 T20220 pobj °C,at
R6114 T20223 T20189 punct ", ",entailing
R6115 T20224 T20189 prep with,entailing
R6116 T20225 T20226 det a,step
R6117 T20226 T20224 pobj step,with
R6118 T20227 T20226 amod final,step
R6119 T20228 T20226 compound extension,step
R6120 T20229 T20226 prep of,step
R6121 T20230 T20231 nummod ten,minutes
R6122 T20231 T20229 pobj minutes,of
R6123 T20232 T20182 punct .,carried
R6124 T20493 T20492 prep of,Isolation
R6125 T20494 T20495 amod pre-implantation,embryos
R6126 T20495 T20493 pobj embryos,of
R6127 T20497 T20498 nsubjpass Time,determined
R6128 T20499 T20497 prep of,Time
R6129 T20500 T20499 pobj fertilization,of
R6130 T20501 T20498 auxpass was,determined
R6131 T20502 T20498 agent by,determined
R6132 T20503 T20502 pobj observation,by
R6133 T20504 T20503 prep of,observation
R6134 T20505 T20506 compound copulation,plugs
R6135 T20506 T20504 pobj plugs,of
R6136 T20507 T20498 punct ", ",determined
R6137 T20508 T20498 cc and,determined
R6138 T20509 T20510 nsubjpass noon,defined
R6139 T20510 T20498 conj defined,determined
R6140 T20511 T20509 prep of,noon
R6141 T20512 T20513 det that,day
R6142 T20513 T20511 pobj day,of
R6143 T20514 T20510 auxpass was,defined
R6144 T20515 T20510 prep as,defined
R6145 T20516 T20515 pobj E0.5,as
R6146 T20517 T20510 punct .,defined
R6147 T20519 T20520 amod Pre-implantation,embryos
R6148 T20520 T20521 nsubjpass embryos,obtained
R6149 T20522 T20521 auxpass were,obtained
R6150 T20523 T20521 prep by,obtained
R6151 T20524 T20523 pcomp dissecting,by
R6152 T20525 T20526 det the,uteri
R6153 T20526 T20524 dobj uteri,dissecting
R6154 T20527 T20524 prep out,dissecting
R6155 T20528 T20527 prep of,out
R6156 T20529 T20530 amod pregnant,females
R6157 T20530 T20528 pobj females,of
R6158 T20531 T20524 prep at,dissecting
R6159 T20532 T20531 pobj E2.5,at
R6160 T20533 T20532 cc or,E2.5
R6161 T20534 T20532 conj E3.5,E2.5
R6162 T20535 T20524 cc and,dissecting
R6163 T20536 T20524 conj flushing,dissecting
R6164 T20537 T20538 det the,oviducts
R6165 T20538 T20536 dobj oviducts,flushing
R6166 T20539 T20536 prep with,flushing
R6167 T20540 T20541 compound ES,medium
R6168 T20541 T20539 pobj medium,with
R6169 T20542 T20541 compound cell,medium
R6170 T20543 T20521 punct .,obtained
R6171 T20545 T20546 amod Isolated,embryos
R6172 T20546 T20547 nsubjpass embryos,digested
R6173 T20548 T20547 auxpass were,digested
R6174 T20549 T20547 advmod then,digested
R6175 T20550 T20547 prep for,digested
R6176 T20551 T20552 nummod five,hours
R6177 T20552 T20550 pobj hours,for
R6178 T20553 T20547 prep at,digested
R6179 T20554 T20555 nummod 55,°C
R6180 T20555 T20553 pobj °C,at
R6181 T20556 T20547 prep in,digested
R6182 T20557 T20558 nummod 20,μL
R6183 T20558 T20556 pobj μL,in
R6184 T20559 T20558 prep of,μL
R6185 T20560 T20561 compound lysis,buffer
R6186 T20561 T20559 pobj buffer,of
R6187 T20562 T20563 punct (,pH
R6188 T20563 T20561 parataxis pH,buffer
R6189 T20564 T20565 nummod 50,mM
R6190 T20565 T20566 compound mM,HCl
R6191 T20566 T20563 dep HCl,pH
R6192 T20567 T20566 compound Tris,HCl
R6193 T20568 T20566 punct -,HCl
R6194 T20569 T20563 punct ", ",pH
R6195 T20570 T20571 nummod 0.5,%
R6196 T20571 T20572 compound %,X
R6197 T20572 T20563 dep X,pH
R6198 T20573 T20572 compound Triton,X
R6199 T20574 T20572 punct -,X
R6200 T20575 T20572 nummod 100,X
R6201 T20576 T20563 punct ", ",pH
R6202 T20577 T20578 nummod 200,μL
R6203 T20578 T20579 nmod μL,K
R6204 T20579 T20563 dep K,pH
R6205 T20580 T20581 punct /,mL
R6206 T20581 T20578 prep mL,μL
R6207 T20582 T20579 compound proteinase,K
R6208 T20583 T20563 punct ", ",pH
R6209 T20584 T20563 nummod 8.0,pH
R6210 T20585 T20563 punct ),pH
R6211 T20586 T20561 punct ", ",buffer
R6212 T20587 T20561 acl followed,buffer
R6213 T20588 T20587 agent by,followed
R6214 T20589 T20590 nummod ten,minutes
R6215 T20590 T20588 pobj minutes,by
R6216 T20591 T20590 prep at,minutes
R6217 T20592 T20593 nummod 95,°C
R6218 T20593 T20591 pobj °C,at
R6219 T20594 T20595 aux to,inactivate
R6220 T20595 T20587 advcl inactivate,followed
R6221 T20596 T20597 det the,K
R6222 T20597 T20595 dobj K,inactivate
R6223 T20598 T20597 compound proteinase,K
R6224 T20599 T20547 punct .,digested
R6225 T20601 T20602 nsubjpass Lysates,used
R6226 T20603 T20602 auxpass were,used
R6227 T20604 T20602 advmod then,used
R6228 T20605 T20602 prep for,used
R6229 T20606 T20607 compound PCR,genotyping
R6230 T20607 T20605 pobj genotyping,for
R6231 T20608 T20602 punct .,used
R6232 T21152 T21151 prep of,Characterization
R6233 T21153 T21154 amod targeted,lines
R6234 T21154 T21152 pobj lines,of
R6235 T21155 T21156 compound ES,cell
R6236 T21156 T21154 compound cell,lines
R6237 T21157 T21151 punct .,Characterization
R6238 T21159 T21160 punct (,A
R6239 T21160 T21161 meta A,detected
R6240 T21162 T21160 punct ),A
R6241 T21163 T21164 amod Targeted,disruption
R6242 T21164 T21161 nsubjpass disruption,detected
R6243 T21165 T21164 prep of,disruption
R6244 T21166 T21167 det the,locus
R6245 T21167 T21165 pobj locus,of
R6246 T21168 T21167 compound Capn2,locus
R6247 T21169 T21161 auxpass was,detected
R6248 T21170 T21161 advmod initially,detected
R6249 T21171 T21161 agent by,detected
R6250 T21172 T21173 compound Southern,blotting
R6251 T21173 T21171 pobj blotting,by
R6252 T21174 T21161 punct .,detected
R6253 T21176 T21177 nsubjpass Membranes,blotted
R6254 T21178 T21177 auxpass were,blotted
R6255 T21179 T21177 prep with,blotted
R6256 T21180 T21181 npadvmod BamHI,digested
R6257 T21181 T21183 amod digested,DNA
R6258 T21182 T21181 punct -,digested
R6259 T21183 T21179 pobj DNA,with
R6260 T21184 T21183 amod genomic,DNA
R6261 T21185 T21183 acl extracted,DNA
R6262 T21186 T21185 prep from,extracted
R6263 T21187 T21188 compound ES,cells
R6264 T21188 T21186 pobj cells,from
R6265 T21189 T21177 cc and,blotted
R6266 T21190 T21177 conj hybridized,blotted
R6267 T21191 T21190 prep with,hybridized
R6268 T21192 T21193 det a,fragment
R6269 T21193 T21191 pobj fragment,with
R6270 T21194 T21195 npadvmod DIG,labeled
R6271 T21195 T21193 amod labeled,fragment
R6272 T21196 T21195 punct -,labeled
R6273 T21197 T21198 nummod 823,bp
R6274 T21198 T21193 compound bp,fragment
R6275 T21199 T21200 compound BamHI,HindIII
R6276 T21200 T21193 compound HindIII,fragment
R6277 T21201 T21200 punct /,HindIII
R6278 T21202 T21193 acl located,fragment
R6279 T21203 T21202 advmod immediately,located
R6280 T21204 T21203 advmod upstream,immediately
R6281 T21205 T21203 prep of,immediately
R6282 T21206 T21207 det the,arm
R6283 T21207 T21205 pobj arm,of
R6284 T21208 T21207 amod short,arm
R6285 T21209 T21207 prep of,arm
R6286 T21210 T21211 det the,vector
R6287 T21211 T21209 pobj vector,of
R6288 T21212 T21211 compound targeting,vector
R6289 T21213 T21214 punct (,Figure
R6290 T21214 T21190 parataxis Figure,hybridized
R6291 T21215 T21214 nummod 1,Figure
R6292 T21216 T21214 punct ),Figure
R6293 T21217 T21177 punct .,blotted
R6294 T21219 T21220 det A,fragment
R6295 T21220 T21225 nsubj fragment,was
R6296 T21221 T21222 nummod 3.5,kb
R6297 T21222 T21220 compound kb,fragment
R6298 T21223 T21222 punct -,kb
R6299 T21224 T21220 compound BamHI,fragment
R6300 T21226 T21220 acl corresponding,fragment
R6301 T21227 T21226 prep to,corresponding
R6302 T21228 T21229 det the,allele
R6303 T21229 T21227 pobj allele,to
R6304 T21230 T21231 amod wild,type
R6305 T21231 T21229 compound type,allele
R6306 T21232 T21231 punct -,type
R6307 T21233 T21225 acomp present,was
R6308 T21234 T21225 prep in,was
R6309 T21235 T21236 det all,cells
R6310 T21236 T21234 pobj cells,in
R6311 T21237 T21225 punct ", ",was
R6312 T21238 T21239 mark whereas,detected
R6313 T21239 T21225 advcl detected,was
R6314 T21240 T21241 det a,fragment
R6315 T21241 T21239 nsubjpass fragment,detected
R6316 T21242 T21243 nummod 5.3,kb
R6317 T21243 T21241 compound kb,fragment
R6318 T21244 T21243 punct -,kb
R6319 T21245 T21241 prep from,fragment
R6320 T21246 T21247 det the,allele
R6321 T21247 T21245 pobj allele,from
R6322 T21248 T21247 compound mutant,allele
R6323 T21249 T21239 auxpass was,detected
R6324 T21250 T21239 prep in,detected
R6325 T21251 T21252 nummod two,lines
R6326 T21252 T21250 pobj lines,in
R6327 T21253 T21252 amod targeted,lines
R6328 T21254 T21252 compound cell,lines
R6329 T21255 T21239 punct ", ",detected
R6330 T21256 T21239 dep designated,detected
R6331 T21257 T21256 oprd ES27,designated
R6332 T21258 T21257 cc and,ES27
R6333 T21259 T21257 conj ES36,ES27
R6334 T21260 T21225 punct .,was
R6335 T21262 T21263 punct (,B
R6336 T21263 T21264 meta B,carried
R6337 T21265 T21263 punct ),B
R6338 T21266 T21267 compound PCR,genotyping
R6339 T21267 T21264 nsubjpass genotyping,carried
R6340 T21268 T21264 auxpass was,carried
R6341 T21269 T21264 prt out,carried
R6342 T21270 T21264 prep with,carried
R6343 T21271 T21272 nummod two,reactions
R6344 T21272 T21270 pobj reactions,with
R6345 T21273 T21272 amod separate,reactions
R6346 T21274 T21272 acl designed,reactions
R6347 T21275 T21276 aux to,amplify
R6348 T21276 T21274 advcl amplify,designed
R6349 T21277 T21278 preconj either,segment
R6350 T21278 T21276 dobj segment,amplify
R6351 T21279 T21278 det a,segment
R6352 T21280 T21281 nummod "2,748",bp
R6353 T21281 T21278 compound bp,segment
R6354 T21282 T21278 prep from,segment
R6355 T21283 T21284 det the,allele
R6356 T21284 T21282 pobj allele,from
R6357 T21285 T21286 amod wild,type
R6358 T21286 T21284 compound type,allele
R6359 T21287 T21286 punct -,type
R6360 T21288 T21278 cc or,segment
R6361 T21289 T21290 det a,segment
R6362 T21290 T21278 conj segment,segment
R6363 T21291 T21292 nummod "2,711",bp
R6364 T21292 T21290 compound bp,segment
R6365 T21293 T21290 prep from,segment
R6366 T21294 T21295 det the,allele
R6367 T21295 T21293 pobj allele,from
R6368 T21296 T21295 compound mutant,allele
R6369 T21297 T21264 punct .,carried
R6370 T21299 T21300 det Both,reactions
R6371 T21300 T21301 nsubj reactions,used
R6372 T21302 T21303 det a,primer
R6373 T21303 T21301 dobj primer,used
R6374 T21304 T21305 amod common,sense
R6375 T21305 T21303 compound sense,primer
R6376 T21306 T21303 acl located,primer
R6377 T21307 T21306 prep in,located
R6378 T21308 T21307 pobj intron,in
R6379 T21309 T21308 nummod 4,intron
R6380 T21310 T21306 punct ", ",located
R6381 T21311 T21306 prep outside,located
R6382 T21312 T21313 det the,arm
R6383 T21313 T21311 pobj arm,outside
R6384 T21314 T21313 amod short,arm
R6385 T21315 T21313 prep of,arm
R6386 T21316 T21317 det the,vector
R6387 T21317 T21315 pobj vector,of
R6388 T21318 T21317 compound targeting,vector
R6389 T21319 T21313 punct ", ",arm
R6390 T21320 T21313 cc and,arm
R6391 T21321 T21322 amod distinct,primers
R6392 T21322 T21313 conj primers,arm
R6393 T21323 T21324 npadvmod allele,specific
R6394 T21324 T21322 amod specific,primers
R6395 T21325 T21324 punct -,specific
R6396 T21326 T21322 compound antisense,primers
R6397 T21327 T21301 punct .,used
R6398 T21329 T21330 det The,reaction
R6399 T21330 T21331 nsubj reaction,used
R6400 T21332 T21333 aux to,detect
R6401 T21333 T21330 advcl detect,reaction
R6402 T21334 T21335 det the,allele
R6403 T21335 T21333 dobj allele,detect
R6404 T21336 T21337 amod wild,type
R6405 T21337 T21335 compound type,allele
R6406 T21338 T21337 punct -,type
R6407 T21339 T21340 det an,primer
R6408 T21340 T21331 dobj primer,used
R6409 T21341 T21340 compound antisense,primer
R6410 T21342 T21340 acl located,primer
R6411 T21343 T21342 prep in,located
R6412 T21344 T21343 pobj exon,in
R6413 T21345 T21344 nummod 7,exon
R6414 T21346 T21347 mark while,done
R6415 T21347 T21331 advcl done,used
R6416 T21348 T21349 det the,amplification
R6417 T21349 T21347 nsubjpass amplification,done
R6418 T21350 T21349 prep of,amplification
R6419 T21351 T21352 det the,sequence
R6420 T21352 T21350 pobj sequence,of
R6421 T21353 T21352 compound mutant,sequence
R6422 T21354 T21347 auxpass was,done
R6423 T21355 T21347 prep with,done
R6424 T21356 T21357 det an,primer
R6425 T21357 T21355 pobj primer,with
R6426 T21358 T21357 compound antisense,primer
R6427 T21359 T21357 prep in,primer
R6428 T21360 T21361 det the,cassette
R6429 T21361 T21359 pobj cassette,in
R6430 T21362 T21363 compound PGK,Neo
R6431 T21363 T21361 compound Neo,cassette
R6432 T21364 T21363 punct -,Neo
R6433 T21365 T21331 punct .,used
R6434 T21367 T21368 det The,results
R6435 T21368 T21369 nsubj results,confirm
R6436 T21370 T21371 det the,presence
R6437 T21371 T21369 dobj presence,confirm
R6438 T21372 T21371 prep of,presence
R6439 T21373 T21374 det the,allele
R6440 T21374 T21372 pobj allele,of
R6441 T21375 T21376 amod wild,type
R6442 T21376 T21374 compound type,allele
R6443 T21377 T21376 punct -,type
R6444 T21378 T21371 prep in,presence
R6445 T21379 T21380 det all,cells
R6446 T21380 T21378 pobj cells,in
R6447 T21381 T21369 punct ", ",confirm
R6448 T21382 T21383 mark whereas,observed
R6449 T21383 T21369 advcl observed,confirm
R6450 T21384 T21385 det the,signal
R6451 T21385 T21383 nsubjpass signal,observed
R6452 T21386 T21385 compound mutant,signal
R6453 T21387 T21385 compound allele,signal
R6454 T21388 T21383 auxpass was,observed
R6455 T21389 T21390 advmod only,in
R6456 T21390 T21383 prep in,observed
R6457 T21391 T21392 det the,clones
R6458 T21392 T21390 pobj clones,in
R6459 T21393 T21392 nummod two,clones
R6460 T21394 T21392 amod targeted,clones
R6461 T21395 T21369 punct .,confirm
R6462 T21397 T21398 punct (,denotes
R6463 T21399 T21398 nsubj M,denotes
R6464 T21400 T21398 punct ),denotes
R6465 T21401 T21402 det the,marker
R6466 T21402 T21398 dobj marker,denotes
R6467 T21403 T21404 amod molecular,weight
R6468 T21404 T21402 compound weight,marker
R6469 T21405 T21398 punct .,denotes
R6470 T21857 T21858 amod Nested,strategy
R6471 T21859 T21858 compound PCR,strategy
R6472 T21860 T21858 prep for,strategy
R6473 T21861 T21860 pobj genotyping,for
R6474 T21862 T21861 prep of,genotyping
R6475 T21863 T21864 amod pre-implantation,embryos
R6476 T21864 T21862 pobj embryos,of
R6477 T21865 T21858 punct .,strategy
R6478 T21867 T21868 prep Due,developed
R6479 T21869 T21867 pcomp to,Due
R6480 T21870 T21871 det the,scarcity
R6481 T21871 T21867 pobj scarcity,Due
R6482 T21872 T21871 prep of,scarcity
R6483 T21873 T21874 amod extractable,material
R6484 T21874 T21872 pobj material,of
R6485 T21875 T21874 amod genetic,material
R6486 T21876 T21868 punct ", ",developed
R6487 T21877 T21878 det a,strategy
R6488 T21878 T21868 nsubjpass strategy,developed
R6489 T21879 T21878 amod nested,strategy
R6490 T21880 T21878 compound PCR,strategy
R6491 T21881 T21868 auxpass was,developed
R6492 T21882 T21868 prep in,developed
R6493 T21883 T21882 pobj order,in
R6494 T21884 T21885 aux to,genotype
R6495 T21885 T21883 acl genotype,order
R6496 T21886 T21887 amod pre-implantation,embryos
R6497 T21887 T21885 dobj embryos,genotype
R6498 T21888 T21868 punct .,developed
R6499 T21890 T21891 amod Separate,reactions
R6500 T21891 T21892 nsubjpass reactions,used
R6501 T21893 T21892 auxpass were,used
R6502 T21894 T21895 aux to,amplify
R6503 T21895 T21892 advcl amplify,used
R6504 T21896 T21897 det a,fragment
R6505 T21897 T21895 dobj fragment,amplify
R6506 T21898 T21899 nummod 429,bp
R6507 T21899 T21897 compound bp,fragment
R6508 T21900 T21897 prep from,fragment
R6509 T21901 T21902 det the,allele
R6510 T21902 T21900 pobj allele,from
R6511 T21903 T21904 amod wild,type
R6512 T21904 T21902 compound type,allele
R6513 T21905 T21904 punct -,type
R6514 T21906 T21897 cc and,fragment
R6515 T21907 T21908 det a,segment
R6516 T21908 T21897 conj segment,fragment
R6517 T21909 T21910 nummod 389,bp
R6518 T21910 T21908 compound bp,segment
R6519 T21911 T21908 prep from,segment
R6520 T21912 T21913 det the,allele
R6521 T21913 T21911 pobj allele,from
R6522 T21914 T21913 compound mutant,allele
R6523 T21915 T21897 punct ", ",fragment
R6524 T21916 T21897 appos both,fragment
R6525 T21917 T21916 acl spanning,both
R6526 T21918 T21919 det the,end
R6527 T21919 T21917 dobj end,spanning
R6528 T21920 T21919 nummod 3,end
R6529 T21921 T21920 punct ',3
R6530 T21922 T21919 prep of,end
R6531 T21923 T21924 det the,arm
R6532 T21924 T21922 pobj arm,of
R6533 T21925 T21924 amod short,arm
R6534 T21926 T21927 punct (,upstream
R6535 T21927 T21924 parataxis upstream,arm
R6536 T21928 T21927 punct ),upstream
R6537 T21929 T21924 prep of,arm
R6538 T21930 T21931 det the,vector
R6539 T21931 T21929 pobj vector,of
R6540 T21932 T21931 compound targeting,vector
R6541 T21933 T21892 punct .,used
R6542 T21935 T21936 prep In,co-amplified
R6543 T21937 T21938 det both,reactions
R6544 T21938 T21935 pobj reactions,In
R6545 T21939 T21936 punct ", ",co-amplified
R6546 T21940 T21941 det a,sequence
R6547 T21941 T21936 nsubjpass sequence,co-amplified
R6548 T21942 T21943 nummod 213,bp
R6549 T21943 T21941 compound bp,sequence
R6550 T21944 T21941 acl located,sequence
R6551 T21945 T21944 prep within,located
R6552 T21946 T21947 det the,arm
R6553 T21947 T21945 pobj arm,within
R6554 T21948 T21947 amod short,arm
R6555 T21949 T21936 auxpass was,co-amplified
R6556 T21950 T21936 prep with,co-amplified
R6557 T21951 T21952 det the,products
R6558 T21952 T21950 pobj products,with
R6559 T21953 T21952 punct ',products
R6560 T21954 T21952 amod diagnostic,products
R6561 T21955 T21952 punct ',products
R6562 T21956 T21936 prep as,co-amplified
R6563 T21957 T21958 det an,control
R6564 T21958 T21956 pobj control,as
R6565 T21959 T21958 amod internal,control
R6566 T21960 T21936 punct .,co-amplified
R6567 T21962 T21963 det The,primers
R6568 T21963 T21966 nsubjpass primers,used
R6569 T21964 T21963 amod same,primers
R6570 T21965 T21963 compound sense,primers
R6571 T21967 T21966 auxpass were,used
R6572 T21968 T21969 aux to,amplify
R6573 T21969 T21966 advcl amplify,used
R6574 T21970 T21969 punct ',amplify
R6575 T21971 T21972 amod diagnostic,sequences
R6576 T21972 T21969 dobj sequences,amplify
R6577 T21973 T21972 punct ',sequences
R6578 T21974 T21972 prep in,sequences
R6579 T21975 T21976 det both,reactions
R6580 T21976 T21974 pobj reactions,in
R6581 T21977 T21969 punct ", ",amplify
R6582 T21978 T21979 mark whereas,were
R6583 T21979 T21969 advcl were,amplify
R6584 T21980 T21981 det the,primers
R6585 T21981 T21979 nsubj primers,were
R6586 T21982 T21981 compound antisense,primers
R6587 T21983 T21984 npadvmod allele,specific
R6588 T21984 T21979 acomp specific,were
R6589 T21985 T21984 punct -,specific
R6590 T21986 T21966 punct .,used
R6591 T21988 T21989 det The,primers
R6592 T21989 T21990 nsubjpass primers,depicted
R6593 T21991 T21989 punct ", ",primers
R6594 T21992 T21989 acl represented,primers
R6595 T21993 T21992 agent by,represented
R6596 T21994 T21993 pobj triangles,by
R6597 T21995 T21990 punct ", ",depicted
R6598 T21996 T21990 auxpass are,depicted
R6599 T21997 T21990 prep in,depicted
R6600 T21998 T21999 nummod two,sets
R6601 T21999 T21997 pobj sets,in
R6602 T22000 T21999 punct (,sets
R6603 T22001 T21999 amod nested,sets
R6604 T22002 T21999 punct ),sets
R6605 T22003 T21999 prep for,sets
R6606 T22004 T22003 pobj each,for
R6607 T22005 T22004 prep of,each
R6608 T22006 T22007 det the,reactions
R6609 T22007 T22005 pobj reactions,of
R6610 T22008 T22007 nummod three,reactions
R6611 T22009 T21990 punct .,depicted
R6612 T22011 T22012 nsubjpass Exons,represented
R6613 T22012 T22014 ccomp represented,cassette
R6614 T22013 T22012 auxpass are,represented
R6615 T22015 T22012 agent by,represented
R6616 T22016 T22017 amod open,rectangles
R6617 T22017 T22015 pobj rectangles,by
R6618 T22018 T22017 punct ", ",rectangles
R6619 T22019 T22017 amod vertical,rectangles
R6620 T22020 T22014 punct ", ",cassette
R6621 T22021 T22014 det the,cassette
R6622 T22022 T22023 compound PGK,Neo
R6623 T22023 T22014 compound Neo,cassette
R6624 T22024 T22023 punct -,Neo
R6625 T22025 T22014 agent by,cassette
R6626 T22026 T22027 det an,rectangle
R6627 T22027 T22025 pobj rectangle,by
R6628 T22028 T22027 amod open,rectangle
R6629 T22029 T22027 punct ", ",rectangle
R6630 T22030 T22027 amod horizontal,rectangle
R6631 T22031 T22014 punct ", ",cassette
R6632 T22032 T22033 mark while,denote
R6633 T22033 T22014 advcl denote,cassette
R6634 T22034 T22035 amod thin,lines
R6635 T22035 T22033 nsubj lines,denote
R6636 T22036 T22035 amod vertical,lines
R6637 T22037 T22038 det the,boundaries
R6638 T22038 T22033 dobj boundaries,denote
R6639 T22039 T22038 prep of,boundaries
R6640 T22040 T22041 det the,arm
R6641 T22041 T22039 pobj arm,of
R6642 T22042 T22041 amod short,arm
R6643 T22043 T22038 cc and,boundaries
R6644 T22044 T22045 det the,end
R6645 T22045 T22038 conj end,boundaries
R6646 T22046 T22045 nummod 5,end
R6647 T22047 T22046 punct ',5
R6648 T22048 T22045 prep of,end
R6649 T22049 T22050 det the,arm
R6650 T22050 T22048 pobj arm,of
R6651 T22051 T22050 amod long,arm
R6652 T22052 T22050 punct (,arm
R6653 T22053 T22050 amod downstream,arm
R6654 T22054 T22050 punct ),arm
R6655 T22055 T22014 punct .,cassette
R6656 T22057 T22058 det A,rectangle
R6657 T22058 T22062 nsubj rectangle,delineates
R6658 T22059 T22058 amod grey,rectangle
R6659 T22060 T22058 punct ", ",rectangle
R6660 T22061 T22058 amod horizontal,rectangle
R6661 T22063 T22064 det the,segment
R6662 T22064 T22062 dobj segment,delineates
R6663 T22065 T22064 prep of,segment
R6664 T22066 T22067 det the,allele
R6665 T22067 T22065 pobj allele,of
R6666 T22068 T22069 amod wild,type
R6667 T22069 T22067 compound type,allele
R6668 T22070 T22069 punct -,type
R6669 T22071 T22072 dep that,replaced
R6670 T22072 T22064 relcl replaced,segment
R6671 T22073 T22072 auxpass is,replaced
R6672 T22074 T22072 agent by,replaced
R6673 T22075 T22076 det the,cassette
R6674 T22076 T22074 pobj cassette,by
R6675 T22077 T22078 compound PGK,Neo
R6676 T22078 T22076 compound Neo,cassette
R6677 T22079 T22078 punct -,Neo
R6678 T22080 T22072 prep in,replaced
R6679 T22081 T22082 det the,allele
R6680 T22082 T22080 pobj allele,in
R6681 T22083 T22082 compound mutant,allele
R6682 T22084 T22062 punct .,delineates
R6683 T22641 T22640 prep of,Genotyping
R6684 T22642 T22643 amod pre-implantation,embryos
R6685 T22643 T22641 pobj embryos,of
R6686 T22644 T22640 punct .,Genotyping
R6687 T22646 T22647 det A,strategy
R6688 T22647 T22650 nsubjpass strategy,used
R6689 T22648 T22647 amod nested,strategy
R6690 T22649 T22647 compound PCR,strategy
R6691 T22651 T22650 auxpass was,used
R6692 T22652 T22653 aux to,genotype
R6693 T22653 T22650 advcl genotype,used
R6694 T22654 T22653 dobj embryos,genotype
R6695 T22655 T22656 amod prior,to
R6696 T22656 T22653 prep to,genotype
R6697 T22657 T22656 pobj implantation,to
R6698 T22658 T22659 mark as,described
R6699 T22659 T22653 advcl described,genotype
R6700 T22660 T22659 prep in,described
R6701 T22661 T22660 pobj Figure,in
R6702 T22662 T22661 nummod 4,Figure
R6703 T22663 T22650 punct .,used
R6704 T22665 T22666 nmod Capn2,mice
R6705 T22666 T22670 nsubjpass mice,mated
R6706 T22667 T22665 punct +,Capn2
R6707 T22668 T22665 punct /,Capn2
R6708 T22669 T22665 punct -,Capn2
R6709 T22671 T22670 auxpass were,mated
R6710 T22672 T22670 cc and,mated
R6711 T22673 T22674 det the,date
R6712 T22674 T22675 nsubj date,established
R6713 T22675 T22670 conj established,mated
R6714 T22676 T22674 prep of,date
R6715 T22677 T22676 pobj fertilization,of
R6716 T22678 T22675 agent by,established
R6717 T22679 T22680 det the,appearance
R6718 T22680 T22678 pobj appearance,by
R6719 T22681 T22680 prep of,appearance
R6720 T22682 T22683 amod vaginal,plugs
R6721 T22683 T22681 pobj plugs,of
R6722 T22684 T22675 punct .,established
R6723 T22686 T22687 nmod Blastocyst,embryos
R6724 T22687 T22695 nsubjpass embryos,flushed
R6725 T22688 T22689 punct (,E3.5
R6726 T22689 T22686 parataxis E3.5,Blastocyst
R6727 T22690 T22689 punct ),E3.5
R6728 T22691 T22686 cc or,Blastocyst
R6729 T22692 T22693 nummod 8,cell
R6730 T22693 T22686 conj cell,Blastocyst
R6731 T22694 T22693 punct -,cell
R6732 T22696 T22697 punct (,E2.5
R6733 T22697 T22687 parataxis E2.5,embryos
R6734 T22698 T22697 punct ),E2.5
R6735 T22699 T22695 auxpass were,flushed
R6736 T22700 T22695 prep from,flushed
R6737 T22701 T22702 det the,oviducts
R6738 T22702 T22700 pobj oviducts,from
R6739 T22703 T22695 cc and,flushed
R6740 T22704 T22705 advmod then,digested
R6741 T22705 T22695 conj digested,flushed
R6742 T22706 T22705 prep with,digested
R6743 T22707 T22708 compound proteinase,K
R6744 T22708 T22706 pobj K,with
R6745 T22709 T22695 punct .,flushed
R6746 T22711 T22712 prep In,co-amplified
R6747 T22713 T22714 amod separate,segments
R6748 T22714 T22711 pobj segments,In
R6749 T22715 T22714 compound reactions,segments
R6750 T22716 T22714 acl found,segments
R6751 T22717 T22716 advmod exclusively,found
R6752 T22718 T22716 prep in,found
R6753 T22719 T22720 preconj either,type
R6754 T22720 T22718 pobj type,in
R6755 T22721 T22720 det the,type
R6756 T22722 T22720 amod wild,type
R6757 T22723 T22720 punct -,type
R6758 T22724 T22720 cc or,type
R6759 T22725 T22720 conj mutant,type
R6760 T22726 T22712 nsubjpass alleles,co-amplified
R6761 T22727 T22712 auxpass were,co-amplified
R6762 T22728 T22712 prep with,co-amplified
R6763 T22729 T22730 det an,sequence
R6764 T22730 T22728 pobj sequence,with
R6765 T22731 T22730 amod internal,sequence
R6766 T22732 T22730 compound control,sequence
R6767 T22733 T22730 punct ", ",sequence
R6768 T22734 T22730 acl located,sequence
R6769 T22735 T22734 prep in,located
R6770 T22736 T22737 det the,arm
R6771 T22737 T22735 pobj arm,in
R6772 T22738 T22737 amod short,arm
R6773 T22739 T22737 punct (,arm
R6774 T22740 T22737 amod upstream,arm
R6775 T22741 T22737 punct ),arm
R6776 T22742 T22737 prep of,arm
R6777 T22743 T22744 det the,vector
R6778 T22744 T22742 pobj vector,of
R6779 T22745 T22744 compound targeting,vector
R6780 T22746 T22744 punct ", ",vector
R6781 T22747 T22748 dep which,found
R6782 T22748 T22744 relcl found,vector
R6783 T22749 T22748 auxpass is,found
R6784 T22750 T22748 prep in,found
R6785 T22751 T22752 det both,alleles
R6786 T22752 T22750 pobj alleles,in
R6787 T22753 T22712 punct .,co-amplified
R6788 T22755 T22756 det The,products
R6789 T22756 T22758 nsubj products,were
R6790 T22757 T22756 amod final,products
R6791 T22759 T22760 nummod 429,bp
R6792 T22760 T22758 attr bp,were
R6793 T22761 T22758 prep for,were
R6794 T22762 T22763 det the,allele
R6795 T22763 T22761 pobj allele,for
R6796 T22764 T22765 amod wild,type
R6797 T22765 T22763 compound type,allele
R6798 T22766 T22765 punct -,type
R6799 T22767 T22758 punct ", ",were
R6800 T22768 T22769 nummod 389,bp
R6801 T22769 T22758 conj bp,were
R6802 T22770 T22769 prep for,bp
R6803 T22771 T22772 det the,allele
R6804 T22772 T22770 pobj allele,for
R6805 T22773 T22772 compound mutant,allele
R6806 T22774 T22769 punct ", ",bp
R6807 T22775 T22769 cc and,bp
R6808 T22776 T22777 nummod 213,bp
R6809 T22777 T22769 conj bp,bp
R6810 T22778 T22777 prep for,bp
R6811 T22779 T22780 det the,control
R6812 T22780 T22778 pobj control,for
R6813 T22781 T22780 amod internal,control
R6814 T22782 T22758 punct .,were
R6815 T22784 T22785 punct (,A
R6816 T22785 T22786 meta A,example
R6817 T22787 T22785 punct ),A
R6818 T22788 T22786 det A,example
R6819 T22789 T22786 amod representative,example
R6820 T22790 T22786 prep of,example
R6821 T22791 T22792 det the,genotyping
R6822 T22792 T22790 pobj genotyping,of
R6823 T22793 T22792 prep of,genotyping
R6824 T22794 T22795 compound blastocyst,embryos
R6825 T22795 T22793 pobj embryos,of
R6826 T22796 T22795 compound stage,embryos
R6827 T22797 T22786 punct .,example
R6828 T22799 T22800 nmod Embryos,1
R6829 T22800 T22802 nsubj 1,were
R6830 T22801 T22800 punct #,1
R6831 T22803 T22800 punct ", ",1
R6832 T22804 T22800 conj 2,1
R6833 T22805 T22804 punct ", ",2
R6834 T22806 T22804 conj 4,2
R6835 T22807 T22806 punct ", ",4
R6836 T22808 T22806 conj 5,4
R6837 T22809 T22808 punct ", ",5
R6838 T22810 T22808 cc and,5
R6839 T22811 T22808 conj 6,5
R6840 T22812 T22802 attr Capn2,were
R6841 T22813 T22812 punct +,Capn2
R6842 T22814 T22812 punct /,Capn2
R6843 T22815 T22812 punct -,Capn2
R6844 T22816 T22817 mark whereas,were
R6845 T22817 T22802 advcl were,were
R6846 T22818 T22819 nmod embryos,3
R6847 T22819 T22817 nsubj 3,were
R6848 T22820 T22819 punct #,3
R6849 T22821 T22819 cc and,3
R6850 T22822 T22823 punct #,7
R6851 T22823 T22819 conj 7,3
R6852 T22824 T22817 attr Capn2,were
R6853 T22825 T22824 punct +,Capn2
R6854 T22826 T22824 punct /,Capn2
R6855 T22827 T22824 punct +,Capn2
R6856 T22828 T22817 punct ", ",were
R6857 T22829 T22817 advcl denoted,were
R6858 T22830 T22829 agent by,denoted
R6859 T22831 T22832 det the,absence
R6860 T22832 T22830 pobj absence,by
R6861 T22833 T22832 prep of,absence
R6862 T22834 T22835 det the,signal
R6863 T22835 T22833 pobj signal,of
R6864 T22836 T22837 nummod 389,bp
R6865 T22837 T22835 compound bp,signal
R6866 T22838 T22835 compound mutant,signal
R6867 T22839 T22802 punct .,were
R6868 T22841 T22842 punct (,B
R6869 T22842 T22843 meta B,shown
R6870 T22844 T22842 punct ),B
R6871 T22845 T22846 det An,example
R6872 T22846 T22843 nsubjpass example,shown
R6873 T22847 T22846 prep of,example
R6874 T22848 T22849 det the,genotyping
R6875 T22849 T22847 pobj genotyping,of
R6876 T22850 T22849 prep of,genotyping
R6877 T22851 T22852 nummod 8,cell
R6878 T22852 T22854 compound cell,embryos
R6879 T22853 T22852 punct -,cell
R6880 T22854 T22850 pobj embryos,of
R6881 T22855 T22843 auxpass is,shown
R6882 T22856 T22843 punct .,shown
R6883 T22858 T22859 nmod Embryos,1
R6884 T22859 T22861 nsubj 1,were
R6885 T22860 T22859 punct #,1
R6886 T22862 T22859 punct ", ",1
R6887 T22863 T22859 conj 2,1
R6888 T22864 T22863 punct ", ",2
R6889 T22865 T22863 conj 3,2
R6890 T22866 T22865 punct ", ",3
R6891 T22867 T22865 conj 5,3
R6892 T22868 T22867 punct ", ",5
R6893 T22869 T22867 cc and,5
R6894 T22870 T22867 conj 6,5
R6895 T22871 T22861 attr Capn2,were
R6896 T22872 T22871 punct +,Capn2
R6897 T22873 T22871 punct /,Capn2
R6898 T22874 T22871 punct -,Capn2
R6899 T22875 T22876 mark while,was
R6900 T22876 T22861 advcl was,were
R6901 T22877 T22876 nsubj embryo,was
R6902 T22878 T22877 punct #,embryo
R6903 T22879 T22877 nummod 4,embryo
R6904 T22880 T22876 attr Capn2,was
R6905 T22881 T22880 punct -,Capn2
R6906 T22882 T22880 punct /,Capn2
R6907 T22883 T22880 punct -,Capn2
R6908 T22884 T22880 punct ", ",Capn2
R6909 T22885 T22880 acl marked,Capn2
R6910 T22886 T22885 agent by,marked
R6911 T22887 T22888 det the,absence
R6912 T22888 T22886 pobj absence,by
R6913 T22889 T22888 prep of,absence
R6914 T22890 T22891 det the,signal
R6915 T22891 T22889 pobj signal,of
R6916 T22892 T22893 nummod 429,bp
R6917 T22893 T22891 nmod bp,signal
R6918 T22894 T22895 amod wild,type
R6919 T22895 T22891 compound type,signal
R6920 T22896 T22895 punct -,type
R6921 T22897 T22861 punct .,were
R6922 T22899 T22900 punct (,M
R6923 T22900 T22901 nsubj M,denotes
R6924 T22902 T22900 punct ),M
R6925 T22903 T22904 det the,marker
R6926 T22904 T22901 dobj marker,denotes
R6927 T22905 T22906 amod molecular,weight
R6928 T22906 T22904 compound weight,marker
R6929 T22907 T22901 punct .,denotes
R6930 T22936 T22937 compound Genotype,distribution
R6931 T22938 T22937 prep of,distribution
R6932 T22939 T22938 pobj offspring,of
R6933 T22940 T22939 acl derived,offspring
R6934 T22941 T22940 prep from,derived
R6935 T22942 T22943 nmod Capn2,mice
R6936 T22943 T22941 pobj mice,from
R6937 T22944 T22943 amod transgenic,mice
R6938 T22945 T22937 punct .,distribution
R6939 T23423 T23424 compound Targeting,strategy
R6940 T23425 T23424 prep for,strategy
R6941 T23426 T23425 pobj disruption,for
R6942 T23427 T23426 prep of,disruption
R6943 T23428 T23429 det the,gene
R6944 T23429 T23427 pobj gene,of
R6945 T23430 T23429 amod murine,gene
R6946 T23431 T23429 compound Capn2,gene
R6947 T23432 T23424 punct .,strategy
R6948 T23434 T23435 det The,gene
R6949 T23435 T23438 nsubjpass gene,disrupted
R6950 T23436 T23435 amod murine,gene
R6951 T23437 T23435 compound Capn2,gene
R6952 T23439 T23435 punct ", ",gene
R6953 T23440 T23435 acl encoding,gene
R6954 T23441 T23442 det the,subunit
R6955 T23442 T23440 dobj subunit,encoding
R6956 T23443 T23444 nmod m,k
R6957 T23444 T23442 compound k,subunit
R6958 T23445 T23444 punct -,k
R6959 T23446 T23444 nummod 80,k
R6960 T23447 T23438 punct ", ",disrupted
R6961 T23448 T23438 auxpass was,disrupted
R6962 T23449 T23438 prep in,disrupted
R6963 T23450 T23451 compound ES,cells
R6964 T23451 T23449 pobj cells,in
R6965 T23452 T23438 agent by,disrupted
R6966 T23453 T23454 amod homologous,recombination
R6967 T23454 T23452 pobj recombination,by
R6968 T23455 T23438 punct .,disrupted
R6969 T23457 T23458 det The,structures
R6970 T23458 T23459 nsubjpass structures,depicted
R6971 T23460 T23458 prep of,structures
R6972 T23461 T23462 det the,allele
R6973 T23462 T23460 pobj allele,of
R6974 T23463 T23464 amod wild,type
R6975 T23464 T23462 compound type,allele
R6976 T23465 T23464 punct -,type
R6977 T23466 T23467 punct (,top
R6978 T23467 T23462 parataxis top,allele
R6979 T23468 T23467 punct ),top
R6980 T23469 T23462 punct ", ",allele
R6981 T23470 T23471 det the,vector
R6982 T23471 T23462 conj vector,allele
R6983 T23472 T23471 compound targeting,vector
R6984 T23473 T23474 punct (,middle
R6985 T23474 T23471 parataxis middle,vector
R6986 T23475 T23474 punct ),middle
R6987 T23476 T23471 punct ", ",vector
R6988 T23477 T23471 cc and,vector
R6989 T23478 T23479 det the,allele
R6990 T23479 T23471 conj allele,vector
R6991 T23480 T23479 compound mutant,allele
R6992 T23481 T23482 punct (,bottom
R6993 T23482 T23479 parataxis bottom,allele
R6994 T23483 T23482 punct ),bottom
R6995 T23484 T23459 auxpass are,depicted
R6996 T23485 T23459 punct .,depicted
R6997 T23487 T23488 prep In,replaces
R6998 T23488 T23498 ccomp replaces,flanked
R6999 T23489 T23490 det the,allele
R7000 T23490 T23487 pobj allele,In
R7001 T23491 T23490 compound mutant,allele
R7002 T23492 T23488 punct ", ",replaces
R7003 T23493 T23494 det a,cassette
R7004 T23494 T23488 nsubj cassette,replaces
R7005 T23495 T23496 compound PGK,Neo
R7006 T23496 T23494 compound Neo,cassette
R7007 T23497 T23496 punct -,Neo
R7008 T23499 T23500 det a,fragment
R7009 T23500 T23488 dobj fragment,replaces
R7010 T23501 T23502 nummod 0.8,kb
R7011 T23502 T23500 nmod kb,fragment
R7012 T23503 T23502 punct -,kb
R7013 T23504 T23500 amod genomic,fragment
R7014 T23505 T23500 acl containing,fragment
R7015 T23506 T23505 dobj exon,containing
R7016 T23507 T23506 nummod 7,exon
R7017 T23508 T23509 punct (,rectangle
R7018 T23509 T23506 parataxis rectangle,exon
R7019 T23510 T23509 amod grey,rectangle
R7020 T23511 T23509 punct ),rectangle
R7021 T23512 T23513 dep which,encodes
R7022 T23513 T23500 relcl encodes,fragment
R7023 T23514 T23515 det the,residue
R7024 T23515 T23513 dobj residue,encodes
R7025 T23516 T23515 amod active,residue
R7026 T23517 T23515 compound site,residue
R7027 T23518 T23515 compound asparagine,residue
R7028 T23519 T23515 punct (,residue
R7029 T23520 T23515 appos Asn286,residue
R7030 T23521 T23498 punct ),flanked
R7031 T23522 T23498 prep In,flanked
R7032 T23523 T23524 det the,vector
R7033 T23524 T23522 pobj vector,In
R7034 T23525 T23524 compound targeting,vector
R7035 T23526 T23498 punct ", ",flanked
R7036 T23527 T23528 det the,cassette
R7037 T23528 T23498 nsubjpass cassette,flanked
R7038 T23529 T23530 compound PGK,Neo
R7039 T23530 T23528 compound Neo,cassette
R7040 T23531 T23530 punct -,Neo
R7041 T23532 T23498 auxpass is,flanked
R7042 T23533 T23498 agent by,flanked
R7043 T23534 T23535 nummod 2.7,kb
R7044 T23535 T23533 pobj kb,by
R7045 T23536 T23535 punct -,kb
R7046 T23537 T23535 prep of,kb
R7047 T23538 T23539 nmod Capn2,sequence
R7048 T23539 T23537 pobj sequence,of
R7049 T23540 T23539 amod homologous,sequence
R7050 T23541 T23498 prep in,flanked
R7051 T23542 T23543 det the,arm
R7052 T23543 T23541 pobj arm,in
R7053 T23544 T23543 amod upstream,arm
R7054 T23545 T23543 punct (,arm
R7055 T23546 T23543 amod short,arm
R7056 T23547 T23543 punct ),arm
R7057 T23548 T23498 cc and,flanked
R7058 T23549 T23550 nummod 7.9,kb
R7059 T23550 T23498 conj kb,flanked
R7060 T23551 T23550 punct -,kb
R7061 T23552 T23550 prep of,kb
R7062 T23553 T23552 pobj homology,of
R7063 T23554 T23550 prep in,kb
R7064 T23555 T23556 det the,arm
R7065 T23556 T23554 pobj arm,in
R7066 T23557 T23556 amod downstream,arm
R7067 T23558 T23556 punct (,arm
R7068 T23559 T23556 amod long,arm
R7069 T23560 T23556 punct ),arm
R7070 T23561 T23498 punct .,flanked
R7071 T23563 T23564 det A,probe
R7072 T23564 T23565 nsubj probe,detects
R7073 T23566 T23564 acl located,probe
R7074 T23567 T23568 advmod immediately,outside
R7075 T23568 T23566 advmod outside,located
R7076 T23569 T23568 prep of,outside
R7077 T23570 T23571 det the,arm
R7078 T23571 T23569 pobj arm,of
R7079 T23572 T23571 amod short,arm
R7080 T23573 T23574 det a,fragment
R7081 T23574 T23565 dobj fragment,detects
R7082 T23575 T23576 nummod 3.5,kb
R7083 T23576 T23574 compound kb,fragment
R7084 T23577 T23576 punct -,kb
R7085 T23578 T23574 compound BamHI,fragment
R7086 T23579 T23565 prep from,detects
R7087 T23580 T23581 det the,allele
R7088 T23581 T23579 pobj allele,from
R7089 T23582 T23583 amod wild,type
R7090 T23583 T23581 compound type,allele
R7091 T23584 T23583 punct -,type
R7092 T23585 T23565 cc and,detects
R7093 T23586 T23587 det a,fragment
R7094 T23587 T23565 conj fragment,detects
R7095 T23588 T23589 nummod 5.3,kb
R7096 T23589 T23587 compound kb,fragment
R7097 T23590 T23589 punct -,kb
R7098 T23591 T23587 compound BamHI,fragment
R7099 T23592 T23587 prep from,fragment
R7100 T23593 T23594 det the,allele
R7101 T23594 T23592 pobj allele,from
R7102 T23595 T23594 compound mutant,allele
R7103 T23596 T23565 punct .,detects
R7104 T23598 T23599 nsubjpass Exons,depicted
R7105 T23600 T23599 auxpass are,depicted
R7106 T23601 T23599 prep as,depicted
R7107 T23602 T23603 amod open,rectangles
R7108 T23603 T23601 pobj rectangles,as
R7109 T23604 T23603 amod vertical,rectangles
R7110 T23605 T23599 prep except,depicted
R7111 T23606 T23605 prep for,except
R7112 T23607 T23606 pobj exon,for
R7113 T23608 T23607 nummod 7,exon
R7114 T23609 T23610 dep which,represented
R7115 T23610 T23607 relcl represented,exon
R7116 T23611 T23610 auxpass is,represented
R7117 T23612 T23610 agent by,represented
R7118 T23613 T23614 det a,rectangle
R7119 T23614 T23612 pobj rectangle,by
R7120 T23615 T23614 amod solid,rectangle
R7121 T23616 T23614 amod vertical,rectangle
R7122 T23617 T23599 punct .,depicted
R7123 T23619 T23620 det The,probe
R7124 T23620 T23621 nsubjpass probe,shown
R7125 T23622 T23620 acl used,probe
R7126 T23623 T23622 prep in,used
R7127 T23624 T23625 amod most,analyses
R7128 T23625 T23623 pobj analyses,in
R7129 T23626 T23627 compound Southern,blot
R7130 T23627 T23625 compound blot,analyses
R7131 T23628 T23621 auxpass is,shown
R7132 T23629 T23621 prep as,shown
R7133 T23630 T23631 det a,rectangle
R7134 T23631 T23629 pobj rectangle,as
R7135 T23632 T23631 amod solid,rectangle
R7136 T23633 T23631 punct ", ",rectangle
R7137 T23634 T23631 amod horizontal,rectangle
R7138 T23635 T23621 punct ", ",shown
R7139 T23636 T23637 mark while,mark
R7140 T23637 T23621 advcl mark,shown
R7141 T23638 T23637 nsubj triangles,mark
R7142 T23639 T23640 det the,positions
R7143 T23640 T23637 dobj positions,mark
R7144 T23641 T23640 prep of,positions
R7145 T23642 T23643 compound PCR,primers
R7146 T23643 T23641 pobj primers,of
R7147 T23644 T23645 advmod also,used
R7148 T23645 T23643 acl used,primers
R7149 T23646 T23645 prep for,used
R7150 T23647 T23648 compound genotyping,purposes
R7151 T23648 T23646 pobj purposes,for
R7152 T23649 T23621 punct .,shown
R7153 T23673 T23674 compound Oligonucleotide,primers
R7154 T23675 T23674 acl used,primers
R7155 T23676 T23677 aux to,genotype
R7156 T23677 T23675 advcl genotype,used
R7157 T23678 T23679 det the,locus
R7158 T23679 T23677 dobj locus,genotype
R7159 T23680 T23679 compound Capn2,locus
R1801 T6919 T6920 nsubj None,were
R1881 T7005 T7006 det "Table 2 Genotype distribution of offspring derived from Capn2 transgenic mice. Cross (N) Age Genotype +/+ +/- -/- ND* (+/-)♀ × (+/-)♂ 199 3 weeks 23 176 0 0 (+/+)♀ × (+/-)♂ 122 3 weeks 50 72 0 0 (+/-)♀ × (+/+)♂ 103 3 weeks 28 75 0 0 (+/-)♀ × (+/-)♂ 9 E18.5 0 7 0 2 (+/-)♀ × (+/-)♂ 5 E17.5 0 5 0 0 (+/-)♀ × (+/-)♂ 9 E14.5 2 7 0 0 (+/-)♀ × (+/-)♂ 10 E11.5 3 7 0 0 (+/-)♀ × (+/-)♂ 7 E10.5 2 5 0 0 (+/-)♀ × (+/-)♂ 46 E3.5 7 39 0 0 (+/-)♀ × (+/-)♂ 48 E2.5 8 34 2 4 * ND, not determined Figure 4 Nested PCR strategy for genotyping of pre-implantation embryos. Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos. Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector. In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control. The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific. The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions. Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm. A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele. Figure 5 Genotyping of pre-implantation embryos. A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4. Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs. Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K. In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles. The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control. (A) A representative example of the genotyping of blastocyst stage embryos. Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal. (B) An example of the genotyping of 8-cell embryos is shown. Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal. (M) denotes the molecular weight marker. The",genotyping
R1882 T7006 T7007 nsubjpass genotyping,shown
R1883 T7008 T7006 nmod results,genotyping
R1884 T7009 T7006 prep for,genotyping
R1885 T7010 T7009 pobj weanlings,for
R1886 T7011 T7010 punct ", ",weanlings
R1887 T7012 T7013 nmod post-implantation,embryos
R1888 T7013 T7010 appos embryos,weanlings
R1889 T7014 T7012 punct ", ",post-implantation
R1890 T7015 T7012 cc and,post-implantation
R1891 T7016 T7012 conj pre-implantation,post-implantation
R5708 T19788 T19789 nummod one,minute
R5709 T19789 T19790 compound minute,denaturation
R5710 T19790 T19787 pobj denaturation,of
R5711 T19791 T19786 prep at,cycles
R5712 T19792 T19793 nummod 95,°C
R5713 T19793 T19791 pobj °C,at
R5714 T19794 T19786 punct ", ",cycles
R5715 T19795 T19796 nummod one,minute
R576 T3568 T3569 amod neuronal,growth
R577 T3569 T3563 conj growth,degeneration
R1059 T4075 T4074 prep to,survival
R1070 T4081 T4079 nsubj mice,represented
R1267 T4295 T4296 det this,stage
R1268 T4296 T4294 pobj stage,at
R1269 T4297 T4296 prep of,stage
R4547 T15138 T15139 amod wild,type
R4548 T15139 T15127 attr type,were
R4549 T15140 T15139 punct -,type
R4550 T15141 T15127 cc and,were
R4551 T15142 T15143 nummod 77.5,%
R4552 T15143 T15144 nsubj %,were
R4553 T15144 T15127 conj were,were
R4554 T15145 T15144 acomp heterozygous,were
R4555 T15146 T15127 punct ", ",were
R4556 T15147 T15148 mark with,observed
R4557 T15148 T15127 advcl observed,were
R4558 T15149 T15150 det no,progeny
R4559 T15150 T15148 nsubj progeny,observed
R4560 T15151 T15152 amod homozygous,null
R4561 T15152 T15150 amod null,progeny
R4562 T15153 T15154 punct [,32
R4563 T15154 T15148 parataxis 32,observed
R4564 T15155 T15154 punct ],32
R4565 T15156 T15127 punct .,were
R4566 T15158 T15159 det These,observations
R4567 T15159 T15160 nsubj observations,suggest
R4568 T15161 T15162 det a,advantage
R4569 T15162 T15160 dobj advantage,suggest
R4570 T15163 T15162 amod developmental,advantage
R4571 T15164 T15162 acl associated,advantage
R4572 T15165 T15164 prep with,associated
R4573 T15166 T15167 amod reduced,expression
R4574 T15167 T15165 pobj expression,with
R4575 T15168 T15167 compound calpain,expression
R4576 T15169 T15160 punct .,suggest
R4577 T15171 T15172 advmod Perhaps,reveal
R4578 T15173 T15174 amod future,studies
R4579 T15174 T15172 nsubj studies,reveal
R4580 T15175 T15172 aux will,reveal
R4581 T15176 T15177 det a,basis
R4582 T15177 T15172 dobj basis,reveal
R4583 T15178 T15177 amod mechanistic,basis
R4584 T15179 T15177 prep for,basis
R4585 T15180 T15179 pobj this,for
R4586 T15181 T15172 punct .,reveal

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T23669 34462-34467 PR:000005015 denotes Capn2
T23668 34433-34440 SO:0000112 denotes primers
T23369 31065-31072 SO:0000112 denotes primers
T23368 30875-30879 SO:0000147 denotes exon
T23367 30817-30822 SO:0000147 denotes Exons
T23366 30809-30815 SO:0001023 denotes allele
T23365 30778-30783 PR:P23940 denotes BamHI
T23364 30758-30764 SO:0001023 denotes allele
T23363 30724-30729 PR:P23940 denotes BamHI
T23362 30547-30552 PR:000005015 denotes Capn2
T23361 30526-30533 SO:0000357 denotes flanked
T23360 30514-30522 SO:0005853 denotes cassette
T23359 30484-30500 SO:0001644 denotes targeting vector
T23358 30437-30448 SO:0100019 denotes active site
T23357 30395-30399 SO:0000147 denotes exon
T23356 30367-30374 SO:0001026 denotes genomic
T22582 12501-12503 SO:0000028 denotes bp
T22581 12458-12463 PR:000005015 denotes Capn2
T22580 12444-12450 UBERON:0000922 denotes embryo
T22579 12429-12434 PR:000005015 denotes Capn2
T22578 12397-12404 UBERON:0000922 denotes Embryos
T22577 12372-12386 UBERON:0019252 denotes 8-cell embryos
T22576 12318-12320 SO:0000028 denotes bp
T22575 12274-12279 PR:000005015 denotes Capn2
T22574 12251-12258 UBERON:0000922 denotes embryos
T22573 12234-12239 PR:000005015 denotes Capn2
T22572 12202-12209 UBERON:0000922 denotes Embryos
T22571 12193-12200 UBERON:0000922 denotes embryos
T22570 12176-12186 UBERON:0000358 denotes blastocyst
T22569 12097-12099 SO:0000028 denotes bp
T22568 12081-12087 SO:0001023 denotes allele
T22567 12063-12065 SO:0000028 denotes bp
T22566 12051-12057 SO:0001023 denotes allele
T22565 12030-12032 SO:0000028 denotes bp
T22564 11993-12000 SO:0001023 denotes alleles
T22563 11952-11968 SO:0001644 denotes targeting vector
T22562 11848-11855 SO:0001023 denotes alleles
T22561 11719-11727 UBERON:0000993 denotes oviducts
T22560 11675-11689 UBERON:0019252 denotes 8-cell embryos
T22559 11654-11664 UBERON:0000358 denotes Blastocyst
T22558 11639-11652 UBERON:0010148 denotes vaginal plugs
T22557 11592-11605 GO:0009566 denotes fertilization
T22556 11570-11575 GO:0007618 denotes mated
T22555 11560-11564 NCBITaxon:10088 denotes mice
T22554 11551-11556 PR:000005015 denotes Capn2
T22553 11512-11524 GO:0007566 denotes implantation
T22552 11495-11502 UBERON:0000922 denotes embryos
T22551 11443-11450 UBERON:0000922 denotes embryos
T22550 11430-11442 GO:0007566 denotes implantation
T21822 11394-11400 SO:0001023 denotes allele
T21821 11371-11379 SO:0005853 denotes cassette
T21820 11332-11338 SO:0001023 denotes allele
T21819 11110-11118 SO:0005853 denotes cassette
T21818 11046-11051 SO:0000147 denotes Exons
T21817 10944-10951 SO:0000112 denotes primers
T21816 10923-10929 SO:0001023 denotes allele
T21815 10910-10917 SO:0000112 denotes primers
T21814 10900-10909 SO:0000077 denotes antisense
T21813 10817-10824 SO:0000112 denotes primers
T21812 10689-10691 SO:0000028 denotes bp
T21811 10646-10662 SO:0001644 denotes targeting vector
T21810 10578-10584 SO:0001023 denotes allele
T21809 10551-10553 SO:0000028 denotes bp
T21808 10534-10540 SO:0001023 denotes allele
T21807 10503-10505 SO:0000028 denotes bp
T21806 10448-10455 UBERON:0000922 denotes embryos
T21805 10435-10447 GO:0007566 denotes implantation
T20470 37860-37872 CHEBI:9750 denotes Triton X-100
T20469 37850-37853 CHEBI:17883 denotes HCl
T20468 37845-37849 CHEBI:9754 denotes Tris
T20467 37825-37830 GO:0019835 denotes lysis
T20466 37763-37770 UBERON:0000922 denotes embryos
T20465 37738-37745 CL:0002322 denotes ES cell
T20464 37724-37732 UBERON:0000993 denotes oviducts
T20463 37674-37682 GO:0007565 denotes pregnant
T20462 37661-37666 UBERON:0000995 denotes uteri
T19358 33987-33993 SO:0000188 denotes intron
T19357 33927-33933 SO:0001023 denotes allele
T19356 33902-33904 SO:0000028 denotes bp
T19355 33883-33889 SO:0001023 denotes allele
T19354 33855-33857 SO:0000028 denotes bp
T19353 33813-33820 UBERON:0000922 denotes embryos
T19352 33800-33812 GO:0007566 denotes implantation
T19351 33769-33777 CL:0002322 denotes ES cells
T19350 33681-33688 SO:0000112 denotes primers
T19349 33631-33638 SO:0001026 denotes genomic
T19348 33467-33475 SO:0005853 denotes cassette
T19347 33428-33430 SO:0000028 denotes bp
T19346 33335-33342 PR:P43870 denotes HindIII
T19345 33329-33334 PR:P23940 denotes BamHI
T19344 33311-33315 SO:0000147 denotes exon
T19343 33308-33310 SO:0000028 denotes bp
T19342 33292-33295 CHEBI:42098 denotes DIG
T19341 33274-33284 GO:0097617 denotes hybridized
T19340 33258-33265 SO:0001026 denotes genomic
T19339 33243-33248 PR:P23940 denotes BamHI
T19338 33163-33166 CHEBI:42098 denotes DIG
T19337 33150-33161 CHEBI:42098 denotes digoxigenin
T19413 37353-37362 GO:0097617 denotes annealing
T19412 37156-37158 SO:0000028 denotes bp
T19411 37122-37124 SO:0000028 denotes bp
T19410 37094-37096 SO:0000028 denotes bp
T19409 37058-37064 SO:0000112 denotes primer
T19408 37048-37057 SO:0000077 denotes antisense
T19407 37039-37045 SO:0000188 denotes intron
T19406 37019-37025 SO:0000112 denotes primer
T19405 37006-37010 SO:0000147 denotes exon
T19404 36981-36987 SO:0000112 denotes primer
T19403 36962-36968 SO:0000112 denotes primer
T19402 36952-36961 SO:0000077 denotes antisense
T19401 36943-36949 SO:0000188 denotes intron
T19400 36929-36935 SO:0000112 denotes primer
T19399 36914-36920 SO:0000188 denotes intron
T19398 36865-36871 SO:0000112 denotes primer
T19397 36846-36853 SO:0001023 denotes alleles
T19396 36778-36794 SO:0001644 denotes targeting vector
T19395 36735-36742 SO:0001026 denotes genomic
T19394 36589-36596 SO:0000112 denotes primers
T19393 36460-36466 SO:0000112 denotes primer
T19392 36450-36459 SO:0000077 denotes antisense
T19391 36436-36442 SO:0000112 denotes primer
T19390 36421-36427 SO:0000188 denotes intron
T19389 36381-36387 SO:0000112 denotes primer
T19388 36360-36368 SO:0005853 denotes cassette
T19387 36338-36344 SO:0000112 denotes primer
T19386 36328-36337 SO:0000077 denotes antisense
T19385 36314-36320 SO:0000112 denotes primer
T19384 36299-36305 SO:0000188 denotes intron
T19383 36270-36276 SO:0000112 denotes primer
T19382 36226-36232 SO:0001023 denotes allele
T19381 36179-36183 SO:0000147 denotes exon
T19380 36169-36175 SO:0000112 denotes primer
T19379 36159-36168 SO:0000077 denotes antisense
T19378 36145-36151 SO:0000112 denotes primer
T19377 36130-36136 SO:0000188 denotes intron
T19376 36023-36029 SO:0000188 denotes intron
T19375 36005-36011 SO:0000112 denotes primer
T19374 35995-36004 SO:0000077 denotes antisense
T19373 35981-35987 SO:0000112 denotes primer
T19372 35966-35972 SO:0000188 denotes intron
T19371 35897-35903 SO:0001023 denotes allele
T19370 35821-35827 SO:0001023 denotes allele
T19369 35755-35761 SO:0001023 denotes allele
T19368 35579-35586 UBERON:0000922 denotes embryos
T19367 35475-35482 UBERON:0000922 denotes embryos
T19366 35462-35474 GO:0007566 denotes implantation
T19365 35428-35435 SO:0000704 denotes genetic
T17959 32962-32968 NCBITaxon:33208 denotes Animal
T17958 32885-32891 NCBITaxon:33208 denotes Animal
T17957 32829-32834 NCBITaxon:10088 denotes Mouse
T17956 32809-32816 SO:0000704 denotes genetic
T17955 32616-32620 UBERON:0010166 denotes coat
T17954 32561-32564 UBERON:0000970 denotes eye
T17393 31264-31273 UBERON:0000922 denotes embryonic
T17392 31258-31263 NCBITaxon:10088 denotes mouse
T17391 31214-31221 CHEBI:5291 denotes gelatin
T17390 31181-31189 CL:0002322 denotes ES cells
T17389 31126-31138 GO:0009294 denotes transfection
T17388 31109-31116 CL:0002322 denotes ES cell
T16645 29534-29539 PR:P23940 denotes BamHI
T16644 29499-29507 SO:0005853 denotes cassette
T16643 29468-29474 SO:0000440 denotes vector
T16642 29420-29425 SO:0000147 denotes exons
T16641 29393-29398 PR:P23940 denotes BamHI
T16640 29385-29392 PR:P43870 denotes HindIII
T16639 29196-29200 SO:0000147 denotes Exon
T16638 29175-29183 SO:0005853 denotes cassette
T16637 29152-29156 SO:0000704 denotes gene
T16636 29146-29151 PR:000005015 denotes Capn2
T16635 29132-29136 SO:0000147 denotes exon
T16634 29103-29110 PR:P43870 denotes HindIII
T16633 29097-29102 PR:P23940 denotes BamHI
T16632 29094-29096 SO:0000028 denotes bp
T16631 29025-29041 SO:0001644 denotes targeting vector
T16630 29019-29024 PR:000005015 denotes Capn2
T16654 29998-30007 SO:0005853 denotes cassettes
T16653 29932-29938 SO:0000188 denotes intron
T16652 29920-29926 SO:0000188 denotes intron
T16651 29882-29889 PR:P43870 denotes HindIII
T16650 29791-29798 SO:0001023 denotes alleles
T16649 29720-29728 SO:0005853 denotes cassette
T16648 29680-29685 PR:P23940 denotes BamHI
T16647 29633-29639 SO:0001023 denotes allele
T16646 29608-29613 PR:P23940 denotes BamHI
T15402 27449-27462 GO:0009790 denotes embryogenesis
T15401 27442-27448 NCBITaxon:39107 denotes murine
T15400 27341-27349 SO:0001060 denotes isoforms
T12390 25813-25821 CL:0002322 denotes ES cells
T12389 25804-25809 PR:000005015 denotes Capn2
T12388 25792-25797 PR:000005015 denotes Capn2
T12387 25773-25788 GO:0035822 denotes gene conversion
T12386 25773-25777 SO:0000704 denotes gene
T12385 25718-25739 CL:0000007 denotes early embryonic cells
T12384 25718-25733 UBERON:0019248 denotes early embryonic
T12383 25520-25526 SO:0001023 denotes allele
T12382 25514-25519 PR:000005022 denotes Capn4
T12381 25176-25182 SO:0001023 denotes allele
T12380 25137-25142 CL:0002322 denotes cells
T12379 25126-25128 _FRAGMENT denotes ES
T12378 25117-25122 PR:000005022 denotes Capn4
T12377 25062-25083 GO:0009790 denotes embryonic development
T12376 25056-25071 UBERON:0019248 denotes early embryonic
T12375 24999-25021 GO:0007059 denotes chromosome segregation
T12374 24946-24968 GO:0007059 denotes chromosome segregation
T12373 24902-24915 GO:0009566 denotes fertilization
T12372 24880-24895 GO:0072687 denotes meiotic spindle
T12371 24880-24887 GO:0007126 denotes meiotic
T12370 24873-24879 CL:0000023 denotes oocyte
T12369 24776-24792 GO:0060473 denotes cortical granule
T12368 24723-24730 CL:0000023 denotes oocytes
T12367 24711-24719 GO:0016020 denotes membrane
T12366 24702-24710 GO:0005938 denotes cortical
T12365 24621-24638 GO:0007340 denotes acrosome reaction
T12364 24621-24629 GO:0001669 denotes acrosome
T12363 24575-24582 CL:0000023 denotes oocytes
T12362 24560-24565 CL:0000019 denotes sperm
T12361 24553-24559 NCBITaxon:9989 denotes rodent
T12360 24476-24493 GO:0007340 denotes acrosome reaction
T12359 24476-24484 GO:0001669 denotes acrosome
T12358 24437-24448 GO:0005737 denotes cytoplasmic
T12357 24411-24417 CL:0000023 denotes oocyte
T12356 24390-24395 CL:0000019 denotes Sperm
T12355 24340-24347 PR:000003090 denotes p27kip1
T12354 24325-24334 PR:000005121 denotes cyclin D1
T12353 24319-24323 PR:000000118 denotes p107
T12352 24314-24317 PR:000003035 denotes p53
T12351 24282-24292 GO:0007049 denotes cell cycle
T12350 24208-24218 GO:0007049 denotes cell cycle
T12349 24192-24200 GO:0051318 denotes G1 stage
T12348 24069-24087 GO:0008283 denotes cell proliferation
T12347 24023-24028 PR:000005022 denotes Capn4
T12346 23821-23826 PR:000005022 denotes Capn4
T12345 23729-23750 GO:0009790 denotes embryonic development
T12344 23729-23738 UBERON:0000922 denotes embryonic
T12343 23682-23687 PR:000005022 denotes Capn4
T12342 23627-23641 GO:0045132 denotes during meiosis
T12341 23573-23595 _FRAGMENT denotes chromosome segregation
T12340 23573-23610 GO:0000070 denotes chromosome segregation during mitosis
T12339 23488-23506 GO:0008283 denotes cell proliferation
T12338 23327-23341 GO:0051301 denotes cell divisions
T12337 23217-23229 UBERON:0019252 denotes 8-cell stage
T12336 23202-23209 UBERON:0000922 denotes embryos
T12335 23193-23198 PR:000005015 denotes Capn2
T12334 23048-23053 PR:000005015 denotes Capn2
T12333 23012-23020 CL:0002322 denotes ES cells
T12332 22964-22971 UBERON:0000922 denotes embryos
T12331 22904-22916 UBERON:0019248 denotes early embryo
T12330 22825-22829 SO:0000704 denotes gene
T12329 22758-22767 UBERON:0000922 denotes embryonic
T12328 22712-22718 UBERON:0000085 denotes morula
T12327 22666-22673 UBERON:0000922 denotes embryos
T12326 22577-22586 UBERON:0000922 denotes embryonic
T12325 22468-22472 SO:0000704 denotes gene
T12324 22430-22442 GO:0007566 denotes implantation
T12323 22394-22399 PR:000005015 denotes Capn2
T12322 22373-22380 UBERON:0000922 denotes embryos
T12321 22360-22372 GO:0007566 denotes implantation
T12320 22227-22239 UBERON:0019252 denotes 8-cell stage
T12319 22201-22208 UBERON:0000922 denotes embryos
T12318 22141-22148 UBERON:0000922 denotes embryos
T12317 22132-22137 PR:000005015 denotes Capn2
T12316 22106-22118 GO:0007566 denotes implantation
T12315 21980-21992 UBERON:0019252 denotes 8-cell stage
T12314 21947-21968 GO:0009790 denotes embryonic development
T12313 21947-21956 UBERON:0000922 denotes embryonic
T12312 21885-21892 UBERON:0000922 denotes embryos
T12311 21876-21881 PR:000005015 denotes Capn2
T12310 21709-21716 NCBITaxon:33208 denotes animals
T12309 21700-21705 PR:000005009 denotes Capn1
T12308 21573-21585 CL:0000232 denotes erythrocytes
T12307 21559-21568 CL:0000233 denotes platelets
T12306 21383-21395 CL:0000232 denotes erythrocytes
T12305 21369-21378 CL:0000233 denotes Platelets
T12304 21212-21221 NCBITaxon:40674 denotes mammalian
T12303 21138-21157 GO:0030168 denotes platelet activation
T12302 21138-21146 CL:0000233 denotes platelet
T12301 21117-21125 GO:0006468 denotes proteins
T12300 21090-21108 _FRAGMENT denotes phosphorylation of
T12299 21033-21041 CL:0000233 denotes platelet
T12298 20990-20995 PR:000005009 denotes Capn1
T12297 20931-20935 NCBITaxon:10088 denotes mice
T12296 20892-20897 PR:000005009 denotes Capn1
T12295 20886-20890 SO:0000704 denotes gene
T12294 20862-20885 PR:000005009 denotes μ-calpain large subunit
T12293 20819-20831 GO:0007566 denotes implantation
T12292 20806-20809 GO:0016265 denotes die
T12291 20798-20805 UBERON:0000922 denotes embryos
T12290 20791-20797 NCBITaxon:39107 denotes murine
T12289 20780-20785 PR:000005015 denotes Capn2
T12288 20747-20760 GO:0009790 denotes embryogenesis
T12287 20663-20667 NCBITaxon:10088 denotes mice
T12286 20635-20639 SO:0000704 denotes gene
T12285 20585-20594 GO:0065007 denotes regulated
T12284 20557-20565 SO:0001060 denotes isoforms
T12283 20499-20503 CHEBI:29108 denotes Ca2+
T12282 20303-20309 MOP:0000780 denotes cleave
T12281 20127-20135 SO:0001060 denotes isoforms
T12280 20005-20009 NCBITaxon:10088 denotes mice
T12279 19891-19898 SO:0000704 denotes genetic
T12278 19830-19835 PR:000005022 denotes Capn4
T12277 19767-19776 NCBITaxon:40674 denotes mammalian
T12276 19753-19760 NCBITaxon:562 denotes E. coli
T12275 19727-19736 GO:0010467 denotes expressed
T12274 19550-19566 CL:0000255 denotes eukaryotic cells
T12273 19550-19560 NCBITaxon:2759 denotes eukaryotic
T12272 19387-19396 NCBITaxon:40674 denotes mammalian
T12271 19346-19353 NCBITaxon:562 denotes E. coli
T12248 18052-18058 SO:0001023 denotes allele
T12247 17973-17978 PR:000005022 denotes Capn4
T12246 17860-17865 SO:0000147 denotes exons
T12245 17755-17760 PR:000005022 denotes Capn4
T12244 17724-17731 NCBITaxon:562 denotes E. coli
T12243 17697-17720 PR:000005015 denotes m-calpain large subunit
T12242 17678-17687 GO:0010467 denotes expressed
T12241 17441-17445 SO:0000147 denotes exon
T12240 17413-17421 SO:0005853 denotes cassette
T12239 17363-17384 PR:000005022 denotes calpain small subunit
T12238 17262-17267 PR:000005022 denotes Capn4
T12237 17102-17111 GO:0007565 denotes gestation
T12236 17021-17028 UBERON:0000922 denotes embryos
T12235 17012-17017 PR:000005022 denotes Capn4
T12234 16964-16971 UBERON:0000922 denotes embryos
T12233 16933-16945 GO:0007566 denotes implantation
T12232 16900-16905 PR:000005022 denotes Capn4
T12231 16771-16775 SO:0000704 denotes gene
T12230 16765-16770 PR:000005022 denotes Capn4
T12229 16715-16728 GO:0009790 denotes embryogenesis
T12228 16590-16602 GO:0007566 denotes implantation
T12227 16576-16579 GO:0016265 denotes die
T12226 16547-16574 PR:000005015 denotes m-calpain catalytic subunit
T12225 16526-16530 NCBITaxon:10088 denotes mice
T12224 16517-16522 PR:000005015 denotes Capn2
T12223 16476-16485 UBERON:0000922 denotes embryonic
T12222 16432-16439 SO:0001060 denotes isoform
T12221 16402-16415 GO:0009790 denotes embryogenesis
T12220 16218-16238 GO:0070527 denotes platelet aggregation
T12219 16218-16226 CL:0000233 denotes platelet
T12218 16154-16181 PR:000005009 denotes μ-calpain catalytic subunit
T12217 16133-16137 NCBITaxon:10088 denotes mice
T12216 16124-16129 PR:000005009 denotes Capn1
T12215 16034-16041 SO:0001060 denotes isoform
T12214 15897-15905 SO:0001060 denotes isoforms
T12213 15866-15887 GO:0009790 denotes embryonic development
T12212 15866-15875 UBERON:0000922 denotes embryonic
T12211 15626-15630 NCBITaxon:10088 denotes mice
T12210 15611-15616 PR:000005022 denotes Capn4
T12209 15588-15597 UBERON:0000922 denotes embryonic
T12208 15498-15508 GO:0065007 denotes regulatory
T12207 15473-15477 SO:0000704 denotes gene
T12206 15467-15472 PR:000005022 denotes Capn4
T12205 15422-15430 SO:0001060 denotes isoforms
T12204 15329-15333 NCBITaxon:10088 denotes mice
T12203 15311-15315 SO:0000704 denotes Gene
T12202 15259-15268 GO:0065007 denotes regulated
T12201 15225-15233 SO:0001060 denotes isoforms
T12200 15016-15026 CHEBI:35222 denotes inhibitors
T2933 152-156 SO:0000704 denotes gene
T2934 178-183 SO:0000704 denotes genes
T2935 187-194 NCBITaxon:40674 denotes mammals
T2936 298-303 PR:000005009 denotes Capn1
T2937 305-311 PR:000005009 denotes μ-80 k
T2938 317-322 PR:000005015 denotes Capn2
T2939 324-330 PR:000005015 denotes m-80 k
T2940 332-337 SO:0000704 denotes genes
T2941 389-399 GO:0065007 denotes regulatory
T2942 423-428 PR:000005022 denotes Capn4
T2943 429-433 SO:0000704 denotes gene
T2944 439-445 _FRAGMENT denotes μ-80 k
T2945 457-465 PR:000005009 denotes subunits
T2946 450-465 PR:000005015 denotes m-80 k subunits
T2947 650-660 GO:0010467 denotes expression
T2948 693-702 NCBITaxon:40674 denotes mammalian
T2949 703-710 UBERON:0000479 denotes tissues
T2950 734-741 SO:0001060 denotes isoform
T2951 826-830 CHEBI:29108 denotes Ca2+
T2952 882-886 CHEBI:29108 denotes Ca2+
T2953 917-921 CHEBI:29108 denotes Ca2+
T2954 1122-1133 GO:0005737 denotes cytoplasmic
T2955 1139-1143 CHEBI:29108 denotes Ca2+
T2956 1252-1262 GO:0065007 denotes regulation
T2957 1326-1336 GO:0065007 denotes regulating
T2958 1581-1589 CL:0000540 denotes neuronal
T2959 1624-1634 GO:0007049 denotes cell cycle
T2960 1717-1725 GO:0016020 denotes membrane
T2961 1760-1772 GO:0005856 denotes cytoskeleton
T2962 1777-1791 GO:0016477 denotes cell migration
T2963 1804-1828 GO:0010941 denotes regulation of cell death
T2964 1851-1860 GO:0006915 denotes apoptosis
T2965 1986-1996 GO:0008219 denotes cell death
T2966 2065-2087 CHEBI:82824 denotes inhibitors of calpains
T2967 2199-2209 GO:0010467 denotes expression
T2968 2213-2224 PR:000005060 denotes calpastatin
T2969 2249-2261 _FRAGMENT denotes inhibitor of
T2970 2271-2278 CHEBI:82824 denotes calpain
T2971 2353-2360 SO:0001060 denotes isoform
T2972 2416-2423 SO:0001060 denotes isoform
T2973 2472-2481 UBERON:0007376 denotes epidermal
T2974 2472-2495 PR:000006928 denotes epidermal growth factor
T2975 2497-2500 PR:000006928 denotes EGF
T2976 2510-2523 GO:0048870 denotes cell motility
T2977 2560-2590 PR:000006065 denotes interferon-inducible protein 9
T2978 2599-2625 GO:0051546 denotes migration of keratinocytes
T2979 2612-2625 CL:0000312 denotes keratinocytes
T2980 2649-2666 CHEBI:82824 denotes calpain inhibitor
T2981 2696-2701 CHEBI:29917 denotes thiol
T2982 2791-2804 GO:0008283 denotes proliferation
T2983 2842-2852 GO:0010467 denotes expression
T2984 2959-2963 SO:0000704 denotes gene
T2985 2976-2980 NCBITaxon:10088 denotes mice
T2986 3107-3114 SO:0001060 denotes isoform
T2987 3160-3165 PR:000005022 denotes Capn4
T2988 3273-3278 PR:000005022 denotes Capn4
T2989 3282-3288 NCBITaxon:39107 denotes murine
T2990 3289-3296 UBERON:0000922 denotes embryos
T2991 3297-3301 GO:0016265 denotes died
T2992 3328-3337 GO:0007565 denotes gestation
T2993 3412-3419 UBERON:0000922 denotes embryos
T2994 3426-3431 PR:000005022 denotes Capn4
T2995 3435-3441 NCBITaxon:39107 denotes murine
T2996 3442-3451 UBERON:0000922 denotes embryonic
T2997 3452-3463 CL:0000057 denotes fibroblasts
T2998 3500-3507 UBERON:0000922 denotes embryos
T2999 3738-3753 GO:0005925 denotes focal adhesions
T3000 3784-3789 PR:000005022 denotes Capn4
T3001 3843-3847 SO:0000704 denotes gene
T3002 3872-3881 UBERON:0000922 denotes embryonic
T3003 3913-3925 GO:0007566 denotes implantation
T3004 3961-3970 UBERON:0000922 denotes embryonic
T3005 4015-4020 PR:000005022 denotes Capn4
T3006 4024-4028 NCBITaxon:10088 denotes mice
T3007 4165-4174 GO:0007565 denotes gestation
T3008 4202-4207 PR:000005022 denotes Capn4
T3009 4211-4215 NCBITaxon:10088 denotes mice
T3010 4269-4274 PR:000005009 denotes Capn1
T3011 4289-4316 PR:000005009 denotes μ-calpain catalytic subunit
T3012 4373-4377 NCBITaxon:10088 denotes mice
T3013 4423-4432 CL:0000233 denotes platelets
T3014 4503-4508 PR:000005022 denotes Capn4
T3015 4514-4518 NCBITaxon:10088 denotes mice
T3016 4519-4522 GO:0016265 denotes die
T3017 4530-4543 GO:0009790 denotes embryogenesis
T3018 4654-4659 PR:000005009 denotes Capn1
T3019 4670-4674 NCBITaxon:10088 denotes mice
T3020 4785-4798 GO:0009790 denotes embryogenesis
T3021 4954-4959 PR:000005015 denotes Capn2
T3022 4960-4964 SO:0000704 denotes gene
T3023 4978-4992 PR:000005015 denotes m-80 k subunit
T3024 4996-5000 NCBITaxon:10088 denotes mice
T3025 5022-5027 PR:000005015 denotes Capn2
T3026 5033-5040 UBERON:0000922 denotes embryos
T3027 5041-5045 GO:0016265 denotes died
T3028 5059-5071 GO:0007566 denotes implantation
T3029 5132-5145 GO:0009790 denotes embryogenesis
T3030 5200-5209 GO:0010467 denotes expressed
T3031 5213-5222 UBERON:0000922 denotes embryonic
T3032 5213-5227 _FRAGMENT denotes embryonic stem
T3033 5233-5238 CL:0002322 denotes cells
T3034 5229-5231 _FRAGMENT denotes ES
T3035 5290-5299 GO:0007565 denotes gestation
T3036 5409-5422 GO:0009790 denotes embryogenesis
T4751 5468-5473 PR:000005015 denotes Capn2
T4752 5483-5490 CL:0002322 denotes ES cell
T4753 5514-5519 PR:000005015 denotes Capn2
T4754 5523-5530 CL:0002322 denotes ES cell
T4755 5599-5603 CHEBI:23888 denotes drug
T4756 5647-5652 PR:000005015 denotes Capn2
T4757 5697-5710 GO:0097617 denotes hybridization
T4758 5790-5800 GO:0097617 denotes hybridized
T4759 5813-5818 PR:P23940 denotes BamHI
T4760 5845-5851 SO:0001023 denotes allele
T4761 5863-5868 PR:P23940 denotes BamHI
T4762 5892-5898 SO:0001023 denotes allele
T4763 5917-5924 SO:0001026 denotes genomic
T4764 6201-6209 SO:0005853 denotes cassette
T4765 6237-6242 PR:P23940 denotes BamHI
T4766 6255-6260 PR:000005015 denotes Capn2
T4767 6264-6272 CL:0002322 denotes ES cells
T4768 6294-6310 SO:0001644 denotes targeting vector
T4769 6340-6345 PR:000005015 denotes Capn2
T4770 6453-6455 SO:0000028 denotes bp
T4771 6468-6470 SO:0000028 denotes bp
T4772 6495-6501 SO:0001023 denotes allele
T4773 6513-6515 SO:0000028 denotes bp
T6380 7903-7907 NCBITaxon:10088 denotes mice
T6381 7948-7953 PR:000005015 denotes Capn2
T6382 7954-7960 SO:0001023 denotes allele
T6383 7981-7985 NCBITaxon:10088 denotes mice
T6384 8003-8009 UBERON:0000085 denotes morula
T6385 8044-8049 PR:000005015 denotes Capn2
T6386 8104-8109 PR:000005015 denotes Capn2
T6387 8185-8190 PR:000005015 denotes Capn2
T6388 8194-8201 NCBITaxon:33208 denotes animals
T6389 8252-8259 UBERON:0001062 denotes anatomy
T6390 8261-8273 GO:0000003 denotes reproduction
T6391 8278-8287 UBERON:0000104 denotes life span
T6392 8345-8350 PR:000005015 denotes Capn2
T6393 8420-8429 UBERON:0012101 denotes perinatal
T6394 8424-8429 GO:0007567 denotes natal
T6395 8430-8435 GO:0016265 denotes death
T6396 8444-8449 PR:000005015 denotes Capn2
T6397 8499-8504 PR:000005015 denotes Capn2
T6398 8508-8515 NCBITaxon:33208 denotes animals
T6399 8516-8524 GO:0016265 denotes perished
T6400 8546-8555 UBERON:0000922 denotes embryonic
T6401 8546-8567 GO:0009790 denotes embryonic development
T6402 8599-8608 UBERON:0000922 denotes embryonic
T6403 8609-8614 GO:0016265 denotes death
T6404 8634-8646 GO:0007566 denotes implantation
T6405 8654-8661 UBERON:0000922 denotes embryos
T6406 8739-8744 PR:000005015 denotes Capn2
T6407 8748-8755 UBERON:0000922 denotes embryos
T6408 8786-8792 UBERON:0000922 denotes embryo
T6409 8853-8858 PR:000005015 denotes Capn2
T6410 8862-8869 UBERON:0000922 denotes embryos
T6411 8879-8884 GO:0016265 denotes dying
T6412 8894-8906 GO:0007566 denotes implantation
T6413 8908-8915 UBERON:0000922 denotes Embryos
T6414 8943-8951 UBERON:0000993 denotes oviducts
T6415 8955-8963 GO:0007565 denotes pregnant
T6416 9086-9098 GO:0007566 denotes implantation
T6417 9099-9106 UBERON:0000922 denotes embryos
T6418 9112-9117 PR:000005015 denotes Capn2
T6419 9157-9162 PR:000005015 denotes Capn2
T6420 9166-9173 UBERON:0000922 denotes embryos
T6421 9195-9207 UBERON:0019252 denotes 8-cell stage
T6422 9301-9311 UBERON:0000358 denotes blastocyst
T6423 9319-9326 UBERON:0000922 denotes embryos
T6424 9332-9337 PR:000005015 denotes Capn2
T6425 9358-9363 PR:000005015 denotes Capn2
T6426 9374-9381 UBERON:0000922 denotes embryos
T6427 9399-9411 UBERON:0019252 denotes 8-cell stage
T6428 9499-9512 UBERON:0019248 denotes early embryos
T6429 9591-9601 SO:0000673 denotes transcript
T6430 9650-9655 PR:000005015 denotes Capn2
T6431 9659-9666 UBERON:0000922 denotes embryos
T7655 14784-14791 CL:0002322 denotes ES cell
T7654 14667-14679 UBERON:0019252 denotes 8-cell stage
T7653 14648-14655 UBERON:0000922 denotes embryos
T7652 14639-14644 PR:000005015 denotes Capn2
T7651 14598-14606 CL:0002322 denotes ES cells
T7650 14589-14594 PR:000005015 denotes Capn2
T7649 14541-14545 CHEBI:23888 denotes drug
T7648 14500-14508 CL:0002322 denotes ES cells
T7647 14491-14496 PR:000005015 denotes Capn2
T7646 14450-14458 CL:0002322 denotes ES cells
T7645 14441-14446 PR:000005022 denotes Capn4
T7644 14392-14400 CL:0002322 denotes ES cells
T7643 14319-14334 GO:0035822 denotes gene conversion
T7642 14319-14323 SO:0000704 denotes gene
T7641 14263-14267 CHEBI:42768 denotes G418
T7640 14192-14200 CL:0002322 denotes ES cells
T7639 14183-14188 PR:000005015 denotes Capn2
T7638 14174-14182 CL:0002322 denotes ES cells
T7637 14165-14170 PR:000005015 denotes Capn2
T7636 14177-14182 GO:0048468 denotes cells
T12270 19333-19342 GO:0010467 denotes expressed
T12269 19217-19227 GO:0010467 denotes expression
T12268 19031-19042 SO:0000673 denotes transcripts
T12267 19001-19009 SO:0000167 denotes promoter
T12266 18977-18989 SO:0000162 denotes splice sites
T12265 18977-18983 GO:0008380 denotes splice
T12264 18969-18976 SO:0000976 denotes cryptic
T12263 18936-18940 SO:0000147 denotes exon
T12262 18832-18838 SO:0001023 denotes allele
T12261 18819-18825 SO:0001023 denotes allele
T12260 18813-18818 PR:000005022 denotes Capn4
T12259 18755-18766 SO:0000673 denotes transcripts
T12258 18703-18708 PR:000005022 denotes Capn4
T12257 18649-18656 SO:0001023 denotes alleles
T12256 18449-18462 GO:0009790 denotes embryogenesis
T12255 18433-18441 GO:0016265 denotes perished
T12254 18425-18432 UBERON:0000922 denotes embryos
T12253 18381-18385 SO:0000704 denotes gene
T12252 18376-18379 PR:000010445 denotes Mll
T12251 18352-18374 PR:000010445 denotes mixed lineage leukemia
T12250 18238-18242 SO:0000704 denotes gene
T12249 18117-18123 SO:0001023 denotes allele
T15409 27876-27880 SO:0000704 denotes gene
T15408 27797-27801 NCBITaxon:10088 denotes mice
T15407 27791-27796 UBERON:0007023 denotes adult
T15406 27770-27783 GO:0009790 denotes embryogenesis
T15405 27757-27769 GO:0007566 denotes implantation
T15404 27667-27675 SO:0001060 denotes isoforms
T15403 27488-27500 GO:0007566 denotes implantation
T15967 28859-28863 SO:0000730 denotes gaps
T15966 28619-28633 SO:0001014 denotes site in intron
T15965 28613-28618 PR:P23940 denotes BamHI
T15964 28591-28605 SO:0001014 denotes site in intron
T15963 28585-28590 PR:P23940 denotes BamHI
T15962 28564-28566 SO:0000028 denotes bp
T15961 28519-28533 SO:0000040 denotes genomic clones
T15960 28496-28497 SO:0000754 denotes λ
T15959 28477-28484 SO:0001026 denotes genomic
T15958 28471-28476 NCBITaxon:10088 denotes mouse
T15957 28392-28404 SO:0000551 denotes polyA signal
T15956 28378-28384 SO:0000204 denotes 3'-UTR
T15955 28334-28344 SO:0000319 denotes stop codon
T15954 28156-28170 PR:000005015 denotes m-80 k subunit
T15953 28150-28155 NCBITaxon:10088 denotes mouse
T15952 28113-28123 SO:0000317 denotes cDNA clone
T15951 28067-28072 SO:0000147 denotes exons
T15950 28040-28046 PR:000005015 denotes m-80 k
T15949 28015-28038 PR:000005015 denotes m-calpain large subunit
T15948 27975-27979 SO:0000704 denotes gene
T15947 27969-27974 PR:000005015 denotes Capn2
T15946 27963-27968 NCBITaxon:10088 denotes mouse
T15945 27947-27952 PR:000005015 denotes Capn2
T15944 27941-27946 NCBITaxon:10088 denotes mouse
T17417 32192-32199 CHEBI:5291 denotes gelatin
T17416 32145-32149 CHEBI:23888 denotes Drug
T17415 32102-32113 CHEBI:465284 denotes ganciclovir
T17414 32075-32079 CHEBI:42768 denotes G418
T17413 32013-32024 GO:0009294 denotes transformed
T17412 31987-31994 CHEBI:5291 denotes gelatin
T17411 31934-31942 CL:0002322 denotes ES cells
T17410 31820-31828 CHEBI:33893 denotes reagents
T17409 31805-31811 UBERON:0000479 denotes tissue
T17408 31708-31715 CHEBI:5291 denotes Gelatin
T17407 31692-31699 CL:0002322 denotes ES cell
T17406 31604-31609 UBERON:0001977 denotes serum
T17405 31597-31603 NCBITaxon:27592 denotes bovine
T17404 31540-31552 CHEBI:2682 denotes amphotericin
T17403 31517-31529 CHEBI:17076 denotes streptomycin
T17402 31495-31505 CHEBI:17334 denotes penicillin
T17401 31467-31484 CHEBI:41218 denotes 2-mercaptoethanol
T17400 31443-31458 CHEBI:50144 denotes sodium pyruvate
T17399 31381-31386 UBERON:0001977 denotes serum
T17398 31374-31380 NCBITaxon:27592 denotes bovine
T17397 31337-31344 CHEBI:17234 denotes glucose
T17396 31310-31317 CL:0002322 denotes ES cell
T17395 31303-31306 CHEBI:16526 denotes CO2
T17394 31274-31285 CL:0000057 denotes fibroblasts
T17953 32546-32551 GO:0007567 denotes birth
T17952 32517-32524 NCBITaxon:33208 denotes animals
T17951 32448-32459 UBERON:0000358 denotes blastocysts
T17950 32392-32399 GO:0007618 denotes matings
T17949 32358-32372 UBERON:0019252 denotes 8-cell embryos
T17948 32318-32326 CL:0002322 denotes ES cells
T17947 32309-32314 PR:000005015 denotes Capn2
T17946 32304-32308 NCBITaxon:10088 denotes mice
T19364 34315-34324 GO:0097617 denotes annealing
T19363 34123-34127 SO:0000147 denotes exon
T19362 34091-34101 GO:0097617 denotes hybridized
T19361 34077-34084 SO:0000112 denotes primers
T19360 34067-34076 SO:0000077 denotes antisense
T19359 34003-34009 SO:0000112 denotes primer
T20461 37621-37628 UBERON:0000922 denotes embryos
T20460 37608-37620 GO:0007566 denotes implantation
T20459 37544-37560 UBERON:0010148 denotes copulation plugs
T20458 37544-37554 GO:0007620 denotes copulation
T20457 37497-37510 GO:0009566 denotes fertilization
T20456 37481-37488 UBERON:0000922 denotes embryos
T20455 37468-37480 GO:0007566 denotes implantation
T21060 6624-6631 CL:0002322 denotes ES cell
T21061 6670-6675 PR:000005015 denotes Capn2
T21062 6755-6760 PR:P23940 denotes BamHI
T21063 6770-6777 SO:0001026 denotes genomic
T21064 6797-6805 CL:0002322 denotes ES cells
T21065 6810-6820 GO:0097617 denotes hybridized
T21066 6828-6831 CHEBI:42098 denotes DIG
T21067 6844-6846 SO:0000028 denotes bp
T21068 6847-6852 PR:P23940 denotes BamHI
T21069 6853-6860 PR:P43870 denotes HindIII
T21070 6923-6939 SO:0001644 denotes targeting vector
T21071 6961-6966 PR:P23940 denotes BamHI
T21072 7007-7013 SO:0001023 denotes allele
T21073 7082-7088 SO:0001023 denotes allele
T21074 7254-7256 SO:0000028 denotes bp
T21075 7284-7290 SO:0001023 denotes allele
T21076 7302-7304 SO:0000028 denotes bp
T21077 7329-7335 SO:0001023 denotes allele
T21078 7372-7378 SO:0000112 denotes primer
T21079 7390-7396 SO:0000188 denotes intron
T21080 7429-7445 SO:0001644 denotes targeting vector
T21081 7460-7466 SO:0001023 denotes allele
T21082 7476-7485 SO:0000077 denotes antisense
T21083 7486-7493 SO:0000112 denotes primers
T21084 7532-7538 SO:0001023 denotes allele
T21085 7547-7556 SO:0000077 denotes antisense
T21086 7557-7563 SO:0000112 denotes primer
T21087 7575-7579 SO:0000147 denotes exon
T21088 7646-7655 SO:0000077 denotes antisense
T21089 7656-7662 SO:0000112 denotes primer
T21090 7678-7686 SO:0005853 denotes cassette
T21091 7738-7744 SO:0001023 denotes allele
T21092 7778-7784 SO:0001023 denotes allele
T21804 10356-10363 SO:0000704 denotes genetic
T21803 10312-10319 UBERON:0000922 denotes embryos
T21802 10299-10311 GO:0007566 denotes implantation
T22930 9756-9761 PR:000005015 denotes Capn2
T22931 9773-9777 NCBITaxon:10088 denotes mice
T23355 30340-30348 SO:0005853 denotes cassette
T23354 30322-30328 SO:0001023 denotes allele
T23353 30278-30284 SO:0001023 denotes allele
T23352 30236-30252 SO:0001644 denotes targeting vector
T23351 30218-30224 SO:0001023 denotes allele
T23350 30148-30156 CL:0002322 denotes ES cells
T23349 30115-30129 PR:000005015 denotes m-80 k subunit
T23348 30096-30100 SO:0000704 denotes gene
T23347 30090-30095 PR:000005015 denotes Capn2
T23346 30083-30089 NCBITaxon:39107 denotes murine
T23345 30073-30077 SO:0000704 denotes gene
T23344 30067-30072 PR:000005015 denotes Capn2
T23343 30060-30066 NCBITaxon:39107 denotes murine
T6447 13259-13266 NCBITaxon:33208 denotes animals
T6446 13250-13255 PR:000005015 denotes Capn2
T6445 13001-13008 NCBITaxon:33208 denotes animals
T6444 12992-12997 PR:000005015 denotes Capn2
T6443 12980-12985 PR:000005015 denotes Capn2
T6442 12944-12951 NCBITaxon:33208 denotes animals
T6441 12935-12940 PR:000005015 denotes Capn2
T6440 12902-12907 PR:000005015 denotes Capn2
T6439 12842-12849 NCBITaxon:33208 denotes animals
T6438 12715-12722 NCBITaxon:33208 denotes animals
T6437 12707-12712 PR:000005015 denotes Capn2
T6436 12695-12700 PR:000005015 denotes Capn2
T6435 12641-12648 UBERON:0000922 denotes embryos
T6434 12628-12640 GO:0007566 denotes implantation
T6433 12606-12618 GO:0007566 denotes implantation
T12396 26063-26072 SO:0000902 denotes transgene
T12395 26045-26050 PR:000005015 denotes Capn2
T7635 14151-14164 _FRAGMENT denotes generation of
T19336 33093-33098 PR:000005015 denotes Capn2
T6466 14111-14117 SO:0001023 denotes allele
T6465 14086-14091 PR:000005015 denotes Capn2
T6464 14016-14022 SO:0001023 denotes allele
T6463 13991-13996 PR:000005015 denotes Capn2
T6462 13865-13872 NCBITaxon:33208 denotes animals
T6461 13856-13861 PR:000005015 denotes Capn2
T6460 13844-13849 PR:000005015 denotes Capn2
T6459 13736-13742 SO:0001023 denotes allele
T6458 13682-13687 PR:000005015 denotes Capn2
T6457 13638-13645 NCBITaxon:33208 denotes animals
T6456 13629-13634 PR:000005015 denotes Capn2
T6455 13566-13573 NCBITaxon:33208 denotes animals
T6454 13557-13562 PR:000005015 denotes Capn2
T6453 13544-13549 PR:000005015 denotes Capn2
T6452 13451-13456 PR:000005022 denotes Capn4
T6451 13411-13418 NCBITaxon:33208 denotes animals
T6450 13331-13338 UBERON:0000922 denotes embryos
T6449 13318-13330 GO:0007566 denotes implantation
T6448 13297-13309 GO:0007566 denotes implantation
T6432 9685-9691 UBERON:0000085 denotes morula
T2931 30-34 CHEBI:29108 denotes Ca2+
T2932 111-119 PR:000005015 denotes capain-2
T12394 26023-26037 PR:000005015 denotes m-80 k subunit
T12393 26011-26018 GO:0010467 denotes express
T12392 25976-25984 CL:0002322 denotes ES cells
T12391 25871-25875 CHEBI:42768 denotes G418
T12407 27126-27136 GO:0010467 denotes expression
T12406 26935-26942 NCBITaxon:33208 denotes animals
T12405 26748-26753 PR:000005022 denotes Capn4
T12404 26604-26608 NCBITaxon:10088 denotes mice
T12403 26469-26474 PR:000005015 denotes Capn2
T12402 26449-26454 PR:000005015 denotes Capn2
T12401 26272-26281 SO:0000902 denotes transgene
T12400 26247-26257 GO:0010467 denotes expression
T12399 26191-26197 SO:0001023 denotes allele
T12398 26082-26097 GO:0035822 denotes gene conversion
T12397 26082-26086 SO:0000704 denotes gene
R2364 T12380 T12379 _lexicallyChainedTo cells,ES
R271 T2945 T2944 _lexicallyChainedTo subunits,μ-80 k
R272 T2970 T2969 _lexicallyChainedTo calpain,inhibitor of
R273 T3033 T3032 _lexicallyChainedTo cells,embryonic stem
R276 T3033 T3034 _lexicallyChainedTo cells,ES
R2219 T7636 T7635 _lexicallyChainedTo cells,generation of
R2359 T12301 T12300 _lexicallyChainedTo proteins,phosphorylation of
R2360 T12342 T12341 _lexicallyChainedTo during meiosis,chromosome segregation

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T23671 34433-34440 SO_EXT:0000112 denotes primers
T23670 34417-34432 CHEBI_SO_EXT:oligonucleotide denotes Oligonucleotide
T23395 30425-30432 SO_EXT:sequence_coding_function denotes encodes
T23394 30395-30399 SO_EXT:0000147 denotes exon
T23393 30367-30374 SO_EXT:0001026 denotes genomic
T23392 30365-30366 CHEBI_SO_EXT:base denotes b
T23391 30349-30357 SO_EXT:sequence_substitution_process denotes replaces
T23390 30340-30348 SO_EXT:0005853 denotes cassette
T23389 30336-30339 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T23388 30332-30335 GO_EXT:phosphoglycerate_kinase denotes PGK
T23387 30322-30328 SO_EXT:0001023 denotes allele
T23386 30315-30321 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T23385 30278-30284 SO_EXT:0001023 denotes allele
T23384 30271-30277 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T23383 30236-30252 SO_EXT:0001644 denotes targeting vector
T23382 30218-30224 SO_EXT:0001023 denotes allele
T23381 30208-30217 SO_EXT:wild_type_entity_or_quality denotes wild-type
T23380 30171-30184 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T23379 30151-30156 CL_GO_EXT:cell denotes cells
T23378 30148-30156 CL:0002322 denotes ES cells
T23377 30115-30129 PR_EXT:000005015 denotes m-80 k subunit
T23376 30102-30110 SO_EXT:sequence_coding_function denotes encoding
T23375 30096-30100 SO_EXT:0000704 denotes gene
T23374 30090-30095 PR_EXT:000005015 denotes Capn2
T23373 30083-30089 NCBITaxon:39107 denotes murine
T23372 30073-30077 SO_EXT:0000704 denotes gene
T23371 30067-30072 PR_EXT:000005015 denotes Capn2
T23370 30060-30066 NCBITaxon:39107 denotes murine
T23422 31065-31072 SO_EXT:0000112 denotes primers
T23421 30938-30943 CHEBI_SO_EXT:molecular_probe denotes probe
T23420 30875-30879 SO_EXT:0000147 denotes exon
T23419 30817-30822 SO_EXT:0000147 denotes Exons
T23418 30809-30815 SO_EXT:0001023 denotes allele
T23417 30802-30808 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T23416 30778-30783 PR_EXT:P23940 denotes BamHI
T23415 30776-30777 CHEBI_SO_EXT:base denotes b
T23414 30758-30764 SO_EXT:0001023 denotes allele
T23413 30748-30757 SO_EXT:wild_type_entity_or_quality denotes wild-type
T23412 30724-30729 PR_EXT:P23940 denotes BamHI
T23411 30722-30723 CHEBI_SO_EXT:base denotes b
T23410 30656-30661 CHEBI_SO_EXT:molecular_probe denotes probe
T23409 30631-30641 SO_EXT:sequence_downstreamness denotes downstream
T23408 30610-30611 CHEBI_SO_EXT:base denotes b
T23407 30580-30588 SO_EXT:sequence_upstreamness denotes upstream
T23406 30564-30572 SO_EXT:biological_sequence denotes sequence
T23405 30547-30552 PR_EXT:000005015 denotes Capn2
T23404 30542-30543 CHEBI_SO_EXT:base denotes b
T23403 30526-30533 SO:0000357 denotes flanked
T23402 30514-30522 SO_EXT:0005853 denotes cassette
T23401 30510-30513 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T23400 30506-30509 GO_EXT:phosphoglycerate_kinase denotes PGK
T23399 30484-30500 SO_EXT:0001644 denotes targeting vector
T23398 30460-30467 CHEBI_EXT:residue denotes residue
T23397 30449-30459 CHEBI_SO_EXT:asparagine denotes asparagine
T23396 30437-30448 SO_EXT:0100019 denotes active site
T22639 12555-12561 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T22638 12538-12547 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T22637 12523-12524 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes M
T22636 12504-12513 SO_EXT:wild_type_entity_or_quality denotes wild-type
T22635 12501-12503 SO_EXT:0000028 denotes bp
T22634 12465-12466 SO_EXT:sequence_nullness_or_absence denotes -
T22633 12463-12464 SO_EXT:sequence_nullness_or_absence denotes -
T22632 12458-12463 PR_EXT:000005015 denotes Capn2
T22631 12444-12450 UBERON:0000922 denotes embryo
T22630 12436-12437 SO_EXT:sequence_nullness_or_absence denotes -
T22629 12434-12435 SO_EXT:normal_or_wild_type_or_present denotes +
T22628 12429-12434 PR_EXT:000005015 denotes Capn2
T22627 12397-12404 UBERON:0000922 denotes Embryos
T22626 12374-12378 CL_GO_EXT:cell denotes cell
T22625 12372-12386 UBERON:0019252 denotes 8-cell embryos
T22624 12321-12327 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T22623 12318-12320 SO_EXT:0000028 denotes bp
T22622 12281-12282 SO_EXT:normal_or_wild_type_or_present denotes +
T22621 12279-12280 SO_EXT:normal_or_wild_type_or_present denotes +
T22620 12274-12279 PR_EXT:000005015 denotes Capn2
T22603 11931-11939 SO_EXT:sequence_upstreamness denotes upstream
T22602 11899-11907 SO_EXT:biological_sequence denotes sequence
T22601 11848-11855 SO_EXT:0001023 denotes alleles
T22600 11841-11847 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T22599 11828-11837 SO_EXT:wild_type_entity_or_quality denotes wild-type
T22598 11751-11761 GO_EXT:0008233 denotes proteinase
T22597 11719-11727 UBERON:0000993 denotes oviducts
T22596 11677-11681 CL_GO_EXT:cell denotes cell
T22595 11675-11689 UBERON:0019252 denotes 8-cell embryos
T22594 11654-11664 UBERON:0000358 denotes Blastocyst
T22593 11639-11652 UBERON:0010148 denotes vaginal plugs
T22592 11592-11605 GO:0009566 denotes fertilization
T22591 11570-11575 GO:0007618 denotes mated
T22590 11560-11564 NCBITaxon:10088 denotes mice
T22589 11558-11559 SO_EXT:sequence_nullness_or_absence denotes -
T22588 11556-11557 SO_EXT:normal_or_wild_type_or_present denotes +
T22587 11551-11556 PR_EXT:000005015 denotes Capn2
T22586 11512-11524 GO:0007566 denotes implantation
T22585 11495-11502 UBERON:0000922 denotes embryos
T22584 11443-11450 UBERON:0000922 denotes embryos
T22583 11430-11442 GO:0007566 denotes implantation
T21834 10625-10633 SO_EXT:sequence_upstreamness denotes upstream
T21833 10578-10584 SO_EXT:0001023 denotes allele
T21832 10571-10577 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T21831 10551-10553 SO_EXT:0000028 denotes bp
T21830 10534-10540 SO_EXT:0001023 denotes allele
T21829 10524-10533 SO_EXT:wild_type_entity_or_quality denotes wild-type
T21828 10503-10505 SO_EXT:0000028 denotes bp
T21827 10448-10455 UBERON:0000922 denotes embryos
T21826 10435-10447 GO:0007566 denotes implantation
T21825 10356-10363 SO_EXT:0000704 denotes genetic
T21824 10312-10319 UBERON:0000922 denotes embryos
T21823 10299-10311 GO:0007566 denotes implantation
T21856 11394-11400 SO_EXT:0001023 denotes allele
T21855 11387-11393 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T21854 11371-11379 SO_EXT:0005853 denotes cassette
T21853 11367-11370 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T21852 11363-11366 GO_EXT:phosphoglycerate_kinase denotes PGK
T21851 11347-11355 SO_EXT:sequence_substitution_process denotes replaced
T21850 11332-11338 SO_EXT:0001023 denotes allele
T21849 11322-11331 SO_EXT:wild_type_entity_or_quality denotes wild-type
T21848 11246-11256 SO_EXT:sequence_downstreamness denotes downstream
T21847 11110-11118 SO_EXT:0005853 denotes cassette
T21846 11106-11109 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T21845 11102-11105 GO_EXT:phosphoglycerate_kinase denotes PGK
T21844 11046-11051 SO_EXT:0000147 denotes Exons
T21843 10944-10951 SO_EXT:0000112 denotes primers
T21842 10923-10929 SO_EXT:0001023 denotes allele
T21841 10910-10917 SO_EXT:0000112 denotes primers
T21840 10900-10909 SO:0000077 denotes antisense
T21839 10859-10868 SO_EXT:biological_sequence denotes sequences
T21838 10817-10824 SO_EXT:0000112 denotes primers
T21837 10692-10700 SO_EXT:biological_sequence denotes sequence
T21836 10689-10691 SO_EXT:0000028 denotes bp
T21835 10646-10662 SO_EXT:0001644 denotes targeting vector
T20491 37957-37967 GO_EXT:0008233 denotes proteinase
T20490 37884-37894 GO_EXT:0008233 denotes proteinase
T20489 37860-37872 CHEBI:9750 denotes Triton X-100
T20488 37850-37853 CHEBI:17883 denotes HCl
T20487 37845-37849 CHEBI:9754 denotes Tris
T20486 37831-37837 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T20485 37825-37830 GO:0019835 denotes lysis
T20484 37763-37770 UBERON:0000922 denotes embryos
T20483 37741-37745 CL_GO_EXT:cell denotes cell
T20482 37738-37745 CL:0002322 denotes ES cell
T20481 37724-37732 UBERON:0000993 denotes oviducts
T20480 37683-37690 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T20479 37674-37682 GO:0007565 denotes pregnant
T20478 37661-37666 UBERON:0000995 denotes uteri
T20477 37621-37628 UBERON:0000922 denotes embryos
T20476 37608-37620 GO:0007566 denotes implantation
T20475 37544-37560 UBERON:0010148 denotes copulation plugs
T20474 37544-37554 GO:0007620 denotes copulation
T20473 37497-37510 GO:0009566 denotes fertilization
T20472 37481-37488 UBERON:0000922 denotes embryos
T20471 37468-37480 GO:0007566 denotes implantation
T19529 37353-37362 GO:0097617 denotes annealing
T19528 37320-37332 GO_EXT:macromolecule_denaturation denotes denaturation
T19527 37274-37286 GO_EXT:macromolecule_denaturation denotes denaturation
T19526 37167-37173 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T19525 37156-37158 SO_EXT:0000028 denotes bp
T19524 37133-37142 SO_EXT:wild_type_entity_or_quality denotes wild-type
T19523 37122-37124 SO_EXT:0000028 denotes bp
T19522 37094-37096 SO_EXT:0000028 denotes bp
T19521 37058-37064 SO_EXT:0000112 denotes primer
T19520 37048-37057 SO:0000077 denotes antisense
T19519 37039-37045 SO_EXT:0000188 denotes intron
T19518 37019-37025 SO_EXT:0000112 denotes primer
T19517 37006-37010 SO_EXT:0000147 denotes exon
T19516 36981-36987 SO_EXT:0000112 denotes primer
T19515 36962-36968 SO_EXT:0000112 denotes primer
T19514 36952-36961 SO:0000077 denotes antisense
T19513 36943-36949 SO_EXT:0000188 denotes intron
T19512 36929-36935 SO_EXT:0000112 denotes primer
T19511 36914-36920 SO_EXT:0000188 denotes intron
T19510 36865-36871 SO_EXT:0000112 denotes primer
T19509 36846-36853 SO_EXT:0001023 denotes alleles
T19508 36839-36845 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T19507 36825-36834 SO_EXT:wild_type_entity_or_quality denotes wild-type
T19506 36778-36794 SO_EXT:0001644 denotes targeting vector
T19505 36735-36742 SO_EXT:0001026 denotes genomic
T19504 36589-36596 SO_EXT:0000112 denotes primers
T19503 36555-36564 SO_EXT:biological_sequence denotes sequences
T19502 36499-36507 SO_EXT:biological_sequence denotes sequence
T19501 36495-36498 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T19500 36491-36494 GO_EXT:phosphoglycerate_kinase denotes PGK
T19499 36460-36466 SO_EXT:0000112 denotes primer
T19498 36450-36459 SO:0000077 denotes antisense
T19497 36436-36442 SO_EXT:0000112 denotes primer
T19496 36421-36427 SO_EXT:0000188 denotes intron
T19495 36381-36387 SO_EXT:0000112 denotes primer
T19494 36360-36368 SO_EXT:0005853 denotes cassette
T19493 36356-36359 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T19415 33093-33098 PR_EXT:000005015 denotes Capn2
T19492 36352-36355 GO_EXT:phosphoglycerate_kinase denotes PGK
T19491 36338-36344 SO_EXT:0000112 denotes primer
T19490 36328-36337 SO:0000077 denotes antisense
T19489 36314-36320 SO_EXT:0000112 denotes primer
T19488 36299-36305 SO_EXT:0000188 denotes intron
T19487 36270-36276 SO_EXT:0000112 denotes primer
T19486 36226-36232 SO_EXT:0001023 denotes allele
T19485 36219-36225 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T19484 36179-36183 SO_EXT:0000147 denotes exon
T19483 36169-36175 SO_EXT:0000112 denotes primer
T19482 36159-36168 SO:0000077 denotes antisense
T19481 36145-36151 SO_EXT:0000112 denotes primer
T19480 36130-36136 SO_EXT:0000188 denotes intron
T19479 36023-36029 SO_EXT:0000188 denotes intron
T19478 36005-36011 SO_EXT:0000112 denotes primer
T19477 35995-36004 SO:0000077 denotes antisense
T19476 35981-35987 SO_EXT:0000112 denotes primer
T19475 35966-35972 SO_EXT:0000188 denotes intron
T19474 35897-35903 SO_EXT:0001023 denotes allele
T19473 35887-35896 SO_EXT:wild_type_entity_or_quality denotes wild-type
T19472 35821-35827 SO_EXT:0001023 denotes allele
T19471 35814-35820 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T19470 35755-35761 SO_EXT:0001023 denotes allele
T19469 35745-35754 SO_EXT:wild_type_entity_or_quality denotes wild-type
T19468 35632-35638 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T19467 35619-35629 GO_EXT:0008233 denotes proteinase
T19466 35579-35586 UBERON:0000922 denotes embryos
T19465 35475-35482 UBERON:0000922 denotes embryos
T19464 35462-35474 GO:0007566 denotes implantation
T19463 35428-35435 SO_EXT:0000704 denotes genetic
T19462 34315-34324 GO:0097617 denotes annealing
T19461 34282-34294 GO_EXT:macromolecule_denaturation denotes denaturation
T19460 34231-34243 GO_EXT:macromolecule_denaturation denotes denaturation
T19459 34151-34159 SO_EXT:biological_sequence denotes sequence
T19458 34146-34149 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T19457 34142-34145 GO_EXT:phosphoglycerate_kinase denotes PGK
T19456 34134-34140 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T19455 34123-34127 SO_EXT:0000147 denotes exon
T19454 34112-34121 SO_EXT:wild_type_entity_or_quality denotes wild-type
T19453 34091-34101 GO:0097617 denotes hybridized
T19452 34077-34084 SO_EXT:0000112 denotes primers
T19451 34067-34076 SO:0000077 denotes antisense
T19450 34003-34009 SO_EXT:0000112 denotes primer
T19449 33987-33993 SO_EXT:0000188 denotes intron
T19448 33927-33933 SO_EXT:0001023 denotes allele
T19447 33920-33926 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T19446 33902-33904 SO_EXT:0000028 denotes bp
T19445 33883-33889 SO_EXT:0001023 denotes allele
T19444 33873-33882 SO_EXT:wild_type_entity_or_quality denotes wild-type
T19443 33855-33857 SO_EXT:0000028 denotes bp
T19442 33813-33820 UBERON:0000922 denotes embryos
T19441 33800-33812 GO:0007566 denotes implantation
T19440 33772-33777 CL_GO_EXT:cell denotes cells
T19439 33769-33777 CL:0002322 denotes ES cells
T19438 33681-33688 SO_EXT:0000112 denotes primers
T19437 33665-33680 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T19436 33648-33657 SO_EXT:biological_sequence denotes sequences
T19435 33639-33642 CHEBI_SO_EXT:DNA denotes DNA
T19434 33631-33642 SO_EXT:genomic_DNA denotes genomic DNA
T19433 33572-33578 SO_EXT:sequence_cloned_entity denotes clones
T19432 33467-33475 SO_EXT:0005853 denotes cassette
T19431 33463-33466 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T19430 33459-33462 GO_EXT:phosphoglycerate_kinase denotes PGK
T19429 33428-33430 SO_EXT:0000028 denotes bp
T19428 33372-33380 SO_EXT:sequence_upstreamness denotes upstream
T19427 33335-33342 PR_EXT:P43870 denotes HindIII
T19426 33329-33334 PR_EXT:P23940 denotes BamHI
T19425 33311-33315 SO_EXT:0000147 denotes exon
T19424 33308-33310 SO_EXT:0000028 denotes bp
T19423 33296-33303 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T19422 33292-33295 CHEBI:42098 denotes DIG
T19421 33274-33284 GO:0097617 denotes hybridized
T19420 33266-33269 CHEBI_SO_EXT:DNA denotes DNA
T19419 33258-33269 SO_EXT:genomic_DNA denotes genomic DNA
T19418 33243-33248 PR_EXT:P23940 denotes BamHI
T19417 33163-33166 CHEBI:42098 denotes DIG
T19416 33150-33161 CHEBI:42098 denotes digoxigenin
T19414 33077-33085 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T17461 32192-32199 CHEBI:5291 denotes gelatin
T17460 32160-32166 SO_EXT:sequence_cloned_entity denotes clones
T17459 32145-32149 CHEBI_EXT:23888 denotes Drug
T17458 32102-32113 CHEBI:465284 denotes ganciclovir
T17457 32075-32079 CHEBI:42768 denotes G418
T17456 32025-32031 SO_EXT:sequence_cloned_entity denotes clones
T17455 32013-32024 GO:0009294 denotes transformed
T17454 31987-31994 CHEBI:5291 denotes gelatin
T17453 31944-31949 CL_GO_EXT:cell denotes Cells
T17452 31937-31942 CL_GO_EXT:cell denotes cells
T17451 31934-31942 CL:0002322 denotes ES cells
T17450 31878-31888 SO_EXT:sequence_linearization denotes linearized
T17449 31864-31873 SO_EXT:engineered_biological_sequence denotes construct
T17448 31820-31828 CHEBI_EXT:33893 denotes reagents
T17447 31805-31811 UBERON:0000479 denotes tissue
T17446 31708-31715 CHEBI:5291 denotes Gelatin
T17445 31700-31706 GO_EXT:biological_growth_entity_or_process denotes growth
T17444 31695-31699 CL_GO_EXT:cell denotes cell
T17443 31692-31699 CL:0002322 denotes ES cell
T17442 31604-31609 UBERON:0001977 denotes serum
T17441 31597-31603 NCBITaxon:27592 denotes bovine
T17440 31540-31552 CHEBI:2682 denotes amphotericin
T17439 31517-31529 CHEBI:17076 denotes streptomycin
T17438 31495-31505 CHEBI:17334 denotes penicillin
T17437 31467-31484 CHEBI:41218 denotes 2-mercaptoethanol
T17987 32962-32968 NCBITaxon:33208 denotes Animal
T17986 32885-32891 NCBITaxon:33208 denotes Animal
T17985 32829-32834 NCBITaxon:10088 denotes Mouse
T17984 32809-32816 SO_EXT:0000704 denotes genetic
T17983 32787-32795 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T17982 32762-32769 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T17981 32745-32749 GO_EXT:breeding denotes bred
T17980 32689-32694 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T17979 32663-32670 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T17978 32649-32653 GO_EXT:breeding denotes bred
T17977 32638-32643 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T17976 32616-32620 UBERON:0010166 denotes coat
T17975 32565-32577 GO_PATO_EXT:biological_pigmentation_process_or_quality denotes pigmentation
T17974 32561-32564 UBERON:0000970 denotes eye
T17973 32546-32551 GO:0007567 denotes birth
T17972 32517-32524 NCBITaxon:33208 denotes animals
T17971 32499-32506 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T17970 32465-32476 GO_EXT:biological_movement_or_translocation_process denotes transferred
T17969 32448-32459 UBERON:0000358 denotes blastocysts
T17968 32392-32399 GO:0007618 denotes matings
T17967 32360-32364 CL_GO_EXT:cell denotes cell
T17966 32358-32372 UBERON:0019252 denotes 8-cell embryos
T17965 32321-32326 CL_GO_EXT:cell denotes cells
T17964 32318-32326 CL:0002322 denotes ES cells
T17963 32316-32317 SO_EXT:sequence_nullness_or_absence denotes -
T17962 32314-32315 SO_EXT:normal_or_wild_type_or_present denotes +
T17961 32309-32314 PR_EXT:000005015 denotes Capn2
T17960 32304-32308 NCBITaxon:10088 denotes mice
T16708 29998-30007 SO_EXT:0005853 denotes cassettes
T16707 29994-29996 GO_PR_EXT:thymidine_kinase denotes tk
T16706 29976-29992 GO_PR_EXT:thymidine_kinase denotes thymidine kinase
T16705 29976-29985 CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes thymidine
T16704 29968-29971 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T16703 29964-29967 GO_EXT:phosphoglycerate_kinase denotes PGK
T16702 29943-29951 SO_EXT:sequence_insertion_process denotes inserted
T16701 29932-29938 SO_EXT:0000188 denotes intron
T16700 29920-29926 SO_EXT:0000188 denotes intron
T16699 29882-29889 PR_EXT:P43870 denotes HindIII
T16698 29880-29881 CHEBI_SO_EXT:base denotes b
T16697 29831-29841 SO_EXT:sequence_downstreamness denotes downstream
T16696 29791-29798 SO_EXT:0001023 denotes alleles
T16679 29445-29453 SO_EXT:sequence_insertion_process denotes inserted
T16677 29393-29398 PR_EXT:P23940 denotes BamHI
T16676 29385-29392 PR_EXT:P43870 denotes HindIII
T16675 29383-29384 CHEBI_SO_EXT:base denotes b
T16674 29350-29359 SO_EXT:engineered_biological_sequence denotes construct
T16673 29319-29327 SO_EXT:sequence_upstreamness denotes upstream
T16672 29298-29306 CHEBI_EXT:residue denotes residues
T16671 29282-29291 GO_MOP_EXT:catalysis denotes catalytic
T16670 29214-29225 CHEBI_SO_EXT:amino_acid denotes amino acids
T16669 29203-29210 SO_EXT:sequence_coding_function denotes encodes
T16668 29196-29200 SO_EXT:0000147 denotes Exon
T16667 29175-29183 SO_EXT:0005853 denotes cassette
T16666 29171-29174 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T16665 29167-29170 GO_EXT:phosphoglycerate_kinase denotes PGK
T16664 29152-29156 SO_EXT:0000704 denotes gene
T16663 29146-29151 PR_EXT:000005015 denotes Capn2
T16662 29132-29136 SO_EXT:0000147 denotes exon
T16661 29103-29110 PR_EXT:P43870 denotes HindIII
T16660 29097-29102 PR_EXT:P23940 denotes BamHI
T16659 29094-29096 SO_EXT:0000028 denotes bp
T16658 29080-29087 SO_EXT:sequence_substitution_process denotes replace
T16657 29054-29063 SO_EXT:engineered_biological_sequence denotes construct
T16656 29025-29041 SO_EXT:0001644 denotes targeting vector
T16655 29019-29024 PR_EXT:000005015 denotes Capn2
T16007 28898-28907 SO_EXT:biological_sequence denotes sequences
T16006 28887-28897 SO_EXT:sequence_repeat_unit_or_region denotes repetitive
T16005 28859-28863 SO_EXT:0000730 denotes gaps
T16004 28772-28781 SO_EXT:biological_sequence denotes sequences
T16003 28644-28650 SO_EXT:sequence_cloned_entity denotes clones
T16002 28619-28633 SO_EXT:0001014 denotes site in intron
T16001 28613-28618 PR_EXT:P23940 denotes BamHI
T16000 28591-28605 SO_EXT:0001014 denotes site in intron
T15999 28585-28590 PR_EXT:P23940 denotes BamHI
T15998 28564-28566 SO_EXT:0000028 denotes bp
T15997 28519-28533 SO_EXT:0000040 denotes genomic clones
T15996 28496-28497 SO_EXT:0000754 denotes λ
T15995 28477-28484 SO_EXT:0001026 denotes genomic
T15994 28471-28476 NCBITaxon:10088 denotes mouse
T15992 28396-28397 CHEBI_EXT:adenine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes A
T15991 28392-28404 SO_EXT:0000551 denotes polyA signal
T15990 28378-28384 SO_EXT:0000204 denotes 3'-UTR
T15989 28334-28344 SO_EXT:0000319 denotes stop codon
T15988 28311-28326 SO_EXT:coding_sequence denotes coding sequence
T15987 28277-28285 SO_EXT:biological_sequence denotes sequence
T15986 28272-28273 CHEBI_SO_EXT:base denotes b
T12812 26935-26942 NCBITaxon:33208 denotes animals
T12811 26894-26902 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T12810 26754-26764 SO_EXT:transgenic_entity denotes transgenic
T12809 26748-26753 PR_EXT:000005022 denotes Capn4
T12808 26604-26608 NCBITaxon:10088 denotes mice
T12807 26577-26586 SO_EXT:wild_type_entity_or_quality denotes wild-type
T12806 26476-26477 SO_EXT:sequence_nullness_or_absence denotes -
T12805 26474-26475 SO_EXT:normal_or_wild_type_or_present denotes +
T12804 26469-26474 PR_EXT:000005015 denotes Capn2
T12803 26456-26457 SO_EXT:normal_or_wild_type_or_present denotes +
T12802 26454-26455 SO_EXT:normal_or_wild_type_or_present denotes +
T12801 26449-26454 PR_EXT:000005015 denotes Capn2
T12800 26402-26410 GO_EXT:breeding denotes breeding
T12799 26272-26281 SO_EXT:0000902 denotes transgene
T12798 26265-26271 SO_EXT:sequence_rescue_process denotes rescue
T12797 26247-26257 GO:0010467 denotes expression
T12796 26191-26197 SO_EXT:0001023 denotes allele
T12795 26181-26190 SO_EXT:wild_type_entity_or_quality denotes wild-type
T12794 26130-26139 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12793 26123-26129 SO_EXT:sequence_rescue_process denotes rescue
T12792 26082-26097 GO:0035822 denotes gene conversion
T12791 26082-26086 SO_EXT:0000704 denotes gene
T12790 26063-26072 SO_EXT:0000902 denotes transgene
T12789 26056-26062 SO_EXT:sequence_rescue_process denotes rescue
T12788 26051-26055 SO_EXT:cDNA denotes cDNA
T12787 26045-26050 PR_EXT:000005015 denotes Capn2
T12786 26023-26037 PR_EXT:000005015 denotes m-80 k subunit
T12785 26011-26018 GO:0010467 denotes express
T12784 25979-25984 CL_GO_EXT:cell denotes cells
T12783 25976-25984 CL:0002322 denotes ES cells
T12782 25956-25962 SO_EXT:sequence_cloned_entity denotes clonal
T12781 25938-25942 CL_GO_EXT:cell denotes cell
T12780 25905-25911 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T12779 25871-25875 CHEBI:42768 denotes G418
T12778 25838-25844 SO_EXT:sequence_cloned_entity denotes clones
T12777 25816-25821 CL_GO_EXT:cell denotes cells
T12776 25813-25821 CL:0002322 denotes ES cells
T12775 25811-25812 SO_EXT:sequence_nullness_or_absence denotes -
T12774 25809-25810 SO_EXT:sequence_nullness_or_absence denotes -
T12773 25804-25809 PR_EXT:000005015 denotes Capn2
T12772 25799-25800 SO_EXT:sequence_nullness_or_absence denotes -
T12771 25797-25798 SO_EXT:normal_or_wild_type_or_present denotes +
T12770 25792-25797 PR_EXT:000005015 denotes Capn2
T12769 25773-25788 GO:0035822 denotes gene conversion
T12768 25773-25777 SO_EXT:0000704 denotes gene
T12767 25734-25739 CL_GO_EXT:cell denotes cells
T12766 25718-25739 CL:0000007 denotes early embryonic cells
T12765 25718-25733 UBERON:0019248 denotes early embryonic
T12764 25688-25692 CL_GO_EXT:cell denotes cell
T12763 25652-25656 CL_GO_EXT:cell denotes cell
T12762 25586-25591 CL_GO_EXT:cell denotes cells
T12761 25520-25526 SO_EXT:0001023 denotes allele
T12760 25514-25519 PR_EXT:000005022 denotes Capn4
T12759 25507-25513 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T12758 25330-25338 GO_PR_EXT:spectrin denotes spectrin
T12757 25176-25182 SO_EXT:0001023 denotes allele
T12756 25137-25142 CL_GO_EXT:cell denotes cells
T12755 25137-25142 CL:0002322 denotes cells
T12754 25126-25128 _FRAGMENT denotes ES
T12753 25124-25125 SO_EXT:sequence_nullness_or_absence denotes -
T12752 25122-25123 SO_EXT:sequence_nullness_or_absence denotes -
T12751 25117-25122 PR_EXT:000005022 denotes Capn4
T12750 25062-25083 GO:0009790 denotes embryonic development
T12749 25056-25071 UBERON:0019248 denotes early embryonic
T12748 25038-25044 GO_EXT:fatality_or_lethality denotes lethal
T12747 24999-25021 GO:0007059 denotes chromosome segregation
T12746 24999-25009 GO_SO_EXT:chromosome denotes chromosome
T12745 24946-24968 GO:0007059 denotes chromosome segregation
T12744 24946-24956 GO_SO_EXT:chromosome denotes chromosome
T12743 24902-24915 GO:0009566 denotes fertilization
T12742 24880-24895 GO:0072687 denotes meiotic spindle
T12725 24553-24559 NCBITaxon:9989 denotes rodent
T12724 24512-24521 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12723 24514-24521 GO_PR_EXT:calpain_1_complex denotes calpain
T12722 24505-24507 _FRAGMENT denotes μ-
T12721 24476-24493 GO:0007340 denotes acrosome reaction
T12720 24476-24484 GO:0001669 denotes acrosome
T12719 24449-24456 CHEBI_EXT:calcium denotes calcium
T12718 24437-24448 GO:0005737 denotes cytoplasmic
T12717 24411-24417 CL:0000023 denotes oocyte
T12716 24396-24403 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12715 24390-24395 CL:0000019 denotes Sperm
T12714 24340-24347 PR_EXT:000003090 denotes p27kip1
T12713 24325-24334 PR_EXT:000005121 denotes cyclin D1
T12712 24319-24323 PR_EXT:000000118 denotes p107
T12711 24314-24317 PR_EXT:000003035 denotes p53
T12710 24282-24292 GO:0007049 denotes cell cycle
T12709 24282-24286 CL_GO_EXT:cell denotes cell
T12708 24249-24257 CHEBI_PR_EXT:protein denotes proteins
T12707 24208-24218 GO:0007049 denotes cell cycle
T12706 24208-24212 CL_GO_EXT:cell denotes cell
T12705 24192-24200 GO:0051318 denotes G1 stage
T12704 24110-24114 CL_GO_EXT:cell denotes cell
T12703 24069-24087 GO:0008283 denotes cell proliferation
T12702 24069-24073 CL_GO_EXT:cell denotes cell
T12701 24030-24031 SO_EXT:sequence_nullness_or_absence denotes -
T12700 24028-24029 SO_EXT:sequence_nullness_or_absence denotes -
T12699 24023-24028 PR_EXT:000005022 denotes Capn4
T12698 24013-24018 CL_GO_EXT:cell denotes cells
T12697 23930-23939 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12696 23932-23939 GO_PR_EXT:calpain_1_complex denotes calpain
T12695 23923-23925 _FRAGMENT denotes μ-
T12694 23845-23849 CL_GO_EXT:cell denotes cell
T12693 23828-23829 SO_EXT:sequence_nullness_or_absence denotes -
T12692 23826-23827 SO_EXT:sequence_nullness_or_absence denotes -
T12691 23821-23826 PR_EXT:000005022 denotes Capn4
T12690 23788-23792 CL_GO_EXT:cell denotes cell
T12689 23729-23750 GO:0009790 denotes embryonic development
T12688 23729-23738 UBERON:0000922 denotes embryonic
T12687 23691-23696 CL_GO_EXT:cell denotes cells
T12686 23689-23690 SO_EXT:sequence_nullness_or_absence denotes -
T12685 23687-23688 SO_EXT:sequence_nullness_or_absence denotes -
T12684 23682-23687 PR_EXT:000005022 denotes Capn4
T12683 23659-23668 GO_EXT:biological_movement_or_translocation_process denotes migration
T12682 23627-23641 GO:0045132 denotes during meiosis
T12681 23573-23595 _FRAGMENT denotes chromosome segregation
T12680 23573-23610 GO:0000070 denotes chromosome segregation during mitosis
T12679 23573-23583 GO_SO_EXT:chromosome denotes chromosome
T12678 23488-23506 GO:0008283 denotes cell proliferation
T12677 23488-23492 CL_GO_EXT:cell denotes cell
T12676 23463-23472 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12675 23399-23408 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12674 23371-23380 GO_EXT:fatality_or_lethality denotes lethality
T12673 23327-23341 GO:0051301 denotes cell divisions
T12672 23327-23331 CL_GO_EXT:cell denotes cell
T12671 23307-23314 CHEBI_PR_EXT:protein denotes protein
T12670 23295-23299 CHEBI_SO_EXT:mRNA denotes mRNA
T12669 23285-23294 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12668 23219-23223 CL_GO_EXT:cell denotes cell
T12667 23217-23229 UBERON:0019252 denotes 8-cell stage
T12666 23202-23209 UBERON:0000922 denotes embryos
T12665 23200-23201 SO_EXT:sequence_nullness_or_absence denotes -
T12664 23198-23199 SO_EXT:sequence_nullness_or_absence denotes -
T12663 23193-23198 PR_EXT:000005015 denotes Capn2
T12662 23155-23159 CL_GO_EXT:cell denotes cell
T12661 23113-23122 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12660 23048-23053 PR_EXT:000005015 denotes Capn2
T12659 23015-23020 CL_GO_EXT:cell denotes cells
T12658 23012-23020 CL:0002322 denotes ES cells
T12657 23005-23011 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T12656 22964-22971 UBERON:0000922 denotes embryos
T12655 22959-22963 SO_EXT:sequence_nullness denotes null
T12654 22904-22916 UBERON:0019248 denotes early embryo
T12653 22891-22896 CL_GO_EXT:cell denotes cells
T12652 22866-22873 GO_EXT:fatality_or_lethality denotes fatally
T12651 22825-22829 SO_EXT:0000704 denotes gene
T12650 22768-22777 SO_EXT:genotype_or_entity_with_genotype denotes genotypes
T12649 22758-22767 UBERON:0000922 denotes embryonic
T12648 22712-22718 UBERON:0000085 denotes morula
T12647 22666-22673 UBERON:0000922 denotes embryos
T12646 22661-22665 SO_EXT:sequence_nullness denotes Null
T12645 22587-22602 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T12644 22577-22586 UBERON:0000922 denotes embryonic
T12643 22468-22472 SO_EXT:0000704 denotes gene
T12642 22443-22452 GO_EXT:fatality_or_lethality denotes lethality
T12641 22430-22442 GO:0007566 denotes implantation
T12640 22401-22402 SO_EXT:sequence_nullness_or_absence denotes -
T12639 22399-22400 SO_EXT:sequence_nullness_or_absence denotes -
T12638 22394-22399 PR_EXT:000005015 denotes Capn2
T12637 22373-22380 UBERON:0000922 denotes embryos
T12636 22360-22372 GO:0007566 denotes implantation
T12635 22229-22233 CL_GO_EXT:cell denotes cell
T12634 22227-22239 UBERON:0019252 denotes 8-cell stage
T12633 22201-22208 UBERON:0000922 denotes embryos
T12632 22196-22200 SO_EXT:sequence_nullness denotes null
T12631 22141-22148 UBERON:0000922 denotes embryos
T12630 22139-22140 SO_EXT:sequence_nullness_or_absence denotes -
T12629 22137-22138 SO_EXT:sequence_nullness_or_absence denotes -
T12628 22132-22137 PR_EXT:000005015 denotes Capn2
T12627 22119-22128 GO_EXT:fatality_or_lethality denotes lethality
T12626 22106-22118 GO:0007566 denotes implantation
T12625 22051-22060 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12624 22039-22046 GO_PR_EXT:calpain_1_complex denotes calpain
T12623 22037-22046 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12622 21982-21986 CL_GO_EXT:cell denotes cell
T12621 21980-21992 UBERON:0019252 denotes 8-cell stage
T12620 21947-21968 GO:0009790 denotes embryonic development
T12619 21947-21956 UBERON:0000922 denotes embryonic
T12618 21911-21920 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12617 21885-21892 UBERON:0000922 denotes embryos
T12616 21883-21884 SO_EXT:sequence_nullness_or_absence denotes -
T12615 21881-21882 SO_EXT:sequence_nullness_or_absence denotes -
T12614 21876-21881 PR_EXT:000005015 denotes Capn2
T12613 21863-21872 GO_EXT:fatality_or_lethality denotes lethality
T12612 21814-21823 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12611 21766-21775 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12610 21709-21716 NCBITaxon:33208 denotes animals
T12609 21707-21708 SO_EXT:sequence_nullness_or_absence denotes -
T12608 21705-21706 SO_EXT:sequence_nullness_or_absence denotes -
T12607 21700-21705 PR_EXT:000005009 denotes Capn1
T12606 21600-21604 CL_GO_EXT:cell denotes cell
T12605 21573-21585 CL:0000232 denotes erythrocytes
T12604 21559-21568 CL:0000233 denotes platelets
T12603 21521-21530 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12602 21497-21506 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12601 21439-21448 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12600 21413-21422 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12599 21383-21395 CL:0000232 denotes erythrocytes
T12598 21369-21378 CL:0000233 denotes Platelets
T12597 21222-21227 CL_GO_EXT:cell denotes cells
T12596 21212-21221 NCBITaxon:40674 denotes mammalian
T12595 21171-21180 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12594 21173-21180 GO_PR_EXT:calpain_1_complex denotes calpain
T12593 21164-21166 _FRAGMENT denotes μ-
T12592 21138-21157 GO:0030168 denotes platelet activation
T12591 21138-21146 CL:0000233 denotes platelet
T12590 21117-21125 CHEBI_PR_EXT:protein denotes proteins
T12589 21117-21125 GO:0006468 denotes proteins
T12588 21090-21108 _FRAGMENT denotes phosphorylation of
T12587 21081-21089 CHEBI_SO_EXT:tyrosine denotes tyrosine
T12586 21033-21041 CL:0000233 denotes platelet
T12585 20990-20995 PR_EXT:000005009 denotes Capn1
T12584 20931-20935 NCBITaxon:10088 denotes mice
T12583 20892-20897 PR_EXT:000005009 denotes Capn1
T12582 20886-20890 SO_EXT:0000704 denotes gene
T12581 20864-20871 GO_PR_EXT:calpain_1_complex denotes calpain
T12580 20862-20885 PR_EXT:000005009 denotes μ-calpain large subunit
T12579 20819-20831 GO:0007566 denotes implantation
T12578 20806-20809 GO:0016265 denotes die
T12577 20798-20805 UBERON:0000922 denotes embryos
T12576 20791-20797 NCBITaxon:39107 denotes murine
T12575 20786-20790 SO_EXT:sequence_nullness denotes null
T12574 20780-20785 PR_EXT:000005015 denotes Capn2
T12573 20747-20760 GO:0009790 denotes embryogenesis
T12572 20710-20723 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T12571 20681-20690 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12570 20683-20690 GO_PR_EXT:calpain_1_complex denotes calpain
T12569 20674-20676 _FRAGMENT denotes μ-
T12568 20663-20667 NCBITaxon:10088 denotes mice
T12567 20635-20639 SO_EXT:0000704 denotes gene
T12566 20602-20607 CL_GO_EXT:cell denotes cells
T12565 20585-20594 GO:0065007 denotes regulated
T12564 20557-20565 SO_EXT:0001060 denotes isoforms
T12563 20499-20503 CHEBI:29108 denotes Ca2+
T12562 20303-20309 MOP:0000780 denotes cleave
T12561 20213-20221 SO_EXT:biological_sequence denotes sequence
T12560 20195-20202 CHEBI_GO_SO_EXT:enzyme denotes enzymes
T12559 20144-20153 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12558 20146-20153 GO_PR_EXT:calpain_1_complex denotes calpain
T12557 20137-20139 _FRAGMENT denotes μ-
T12556 20127-20135 SO_EXT:0001060 denotes isoforms
T12555 20005-20009 NCBITaxon:10088 denotes mice
T12554 19994-20004 SO_EXT:transgenic_entity denotes transgenic
T12553 19922-19932 SO_EXT:transgenic_entity denotes transgenic
T12552 19891-19898 SO_EXT:0000704 denotes genetic
T12551 19830-19835 PR_EXT:000005022 denotes Capn4
T12550 19813-19822 GO_EXT:fatality_or_lethality denotes lethality
T12548 19767-19776 NCBITaxon:40674 denotes mammalian
T12547 19753-19760 NCBITaxon:562 denotes E. coli
T12546 19727-19736 GO:0010467 denotes expressed
T12545 19561-19566 CL_GO_EXT:cell denotes cells
T12544 19550-19566 CL:0000255 denotes eukaryotic cells
T12543 19550-19560 NCBITaxon:2759 denotes eukaryotic
T12542 19397-19402 CL_GO_EXT:cell denotes cells
T12541 19387-19396 NCBITaxon:40674 denotes mammalian
T12540 19346-19353 NCBITaxon:562 denotes E. coli
T12539 19333-19342 GO:0010467 denotes expressed
T12538 19251-19259 SO_EXT:sequence_alteration_process denotes modified
T12537 19217-19227 GO:0010467 denotes expression
T12536 19131-19136 CHEBI_SO_EXT:amino_acid denotes acids
T12535 19120-19130 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T12534 19031-19042 SO_EXT:0000673 denotes transcripts
T12533 19010-19018 SO_EXT:biological_sequence denotes sequence
T12532 19001-19009 SO_EXT:0000167 denotes promoter
T12531 18997-19000 GO_EXT:phosphoglycerate_kinase denotes PGK
T12530 18977-18989 SO_EXT:0000162 denotes splice sites
T12529 18977-18983 GO:0008380 denotes splice
T12528 18969-18976 SO:0000976 denotes cryptic
T12527 18936-18940 SO_EXT:0000147 denotes exon
T12526 18889-18891 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T12525 18861-18865 CHEBI_SO_EXT:mRNA denotes mRNA
T12524 18832-18838 SO_EXT:0001023 denotes allele
T12523 18819-18825 SO_EXT:0001023 denotes allele
T12522 18813-18818 PR_EXT:000005022 denotes Capn4
T12521 18755-18766 SO_EXT:0000673 denotes transcripts
T12520 18709-18719 SO_EXT:transgenic_entity denotes transgenic
T12519 18703-18708 PR_EXT:000005022 denotes Capn4
T12518 18649-18656 SO_EXT:0001023 denotes alleles
T12517 18642-18648 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T12516 18615-18623 CHEBI_PR_EXT:protein denotes proteins
T12515 18605-18614 SO_EXT:sequence_truncation_process denotes truncated
T12514 18449-18462 GO:0009790 denotes embryogenesis
T12513 18433-18441 GO:0016265 denotes perished
T12512 18425-18432 UBERON:0000922 denotes embryos
T12511 18420-18424 SO_EXT:sequence_nullness denotes null
T12510 18381-18385 SO_EXT:0000704 denotes gene
T12741 24880-24887 GO:0007126 denotes meiotic
T12740 24873-24879 CL:0000023 denotes oocyte
T12739 24856-24865 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T12738 24839-24848 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12737 24776-24792 GO:0060473 denotes cortical granule
T12736 24765-24772 GO_EXT:biological_release denotes release
T12735 24723-24730 CL:0000023 denotes oocytes
T12734 24711-24719 GO:0016020 denotes membrane
T12733 24702-24710 GO:0005938 denotes cortical
T12732 24685-24694 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12731 24668-24681 GO_EXT:biological_translocation_entity_or_process denotes translocation
T12730 24621-24638 GO:0007340 denotes acrosome reaction
T12729 24621-24629 GO:0001669 denotes acrosome
T12728 24589-24598 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12727 24575-24582 CL:0000023 denotes oocytes
T12726 24560-24565 CL:0000019 denotes sperm
T12509 18376-18379 PR_EXT:000010445 denotes Mll
T12508 18352-18374 PR_EXT:000010445 denotes mixed lineage leukemia
T12507 18238-18242 SO_EXT:0000704 denotes gene
T12506 18148-18156 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T12505 18117-18123 SO_EXT:0001023 denotes allele
T12504 18091-18099 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T12503 18086-18090 SO_EXT:sequence_nullness denotes null
T12502 18052-18058 SO_EXT:0001023 denotes allele
T12501 17973-17978 PR_EXT:000005022 denotes Capn4
T12500 17941-17950 GO_EXT:fatality_or_lethality denotes lethality
T12499 17860-17865 SO_EXT:0000147 denotes exons
T12498 17848-17856 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T12497 17755-17760 PR_EXT:000005022 denotes Capn4
T12496 17724-17731 NCBITaxon:562 denotes E. coli
T12495 17697-17720 PR_EXT:000005015 denotes m-calpain large subunit
T12494 17697-17706 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12493 17678-17687 GO:0010467 denotes expressed
T12492 17613-17621 CHEBI_EXT:residue denotes residues
T12491 17602-17612 CHEBI_SO_EXT:amino_acid denotes amino acid
T12490 17588-17598 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T12489 17572-17580 SO_EXT:sequence_deletion_process denotes excision
T12488 17480-17487 CHEBI_PR_EXT:protein denotes protein
T12487 17462-17472 SO_EXT:sequence_truncation_entity_or_process denotes truncation
T12486 17441-17445 SO_EXT:0000147 denotes exon
T12485 17413-17421 SO_EXT:0005853 denotes cassette
T12484 17409-17412 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T12483 17405-17408 GO_EXT:phosphoglycerate_kinase denotes PGK
T12482 17388-17397 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T12481 17363-17384 PR_EXT:000005022 denotes calpain small subunit
T12480 17345-17355 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T12479 17262-17267 PR_EXT:000005022 denotes Capn4
T12478 17252-17256 SO_EXT:null_sequence_entity denotes null
T12477 17102-17111 GO:0007565 denotes gestation
T12476 17021-17028 UBERON:0000922 denotes embryos
T12475 17019-17020 SO_EXT:sequence_nullness_or_absence denotes -
T12474 17017-17018 SO_EXT:sequence_nullness_or_absence denotes -
T12473 17012-17017 PR_EXT:000005022 denotes Capn4
T12472 16964-16971 UBERON:0000922 denotes embryos
T12471 16959-16963 SO_EXT:sequence_nullness denotes null
T12470 16946-16955 GO_EXT:fatality_or_lethality denotes lethality
T12469 16933-16945 GO:0007566 denotes implantation
T12468 16900-16905 PR_EXT:000005022 denotes Capn4
T12467 16805-16814 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12466 16771-16775 SO_EXT:0000704 denotes gene
T12465 16765-16770 PR_EXT:000005022 denotes Capn4
T12464 16715-16728 GO:0009790 denotes embryogenesis
T12463 16673-16682 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12462 16590-16602 GO:0007566 denotes implantation
T12461 16576-16579 GO:0016265 denotes die
T12460 16557-16566 GO_MOP_EXT:catalysis denotes catalytic
T12459 16547-16574 PR_EXT:000005015 denotes m-calpain catalytic subunit
T12458 16547-16556 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12457 16526-16530 NCBITaxon:10088 denotes mice
T12456 16524-16525 SO_EXT:sequence_nullness_or_absence denotes -
T12455 16522-16523 SO_EXT:sequence_nullness_or_absence denotes -
T12454 16517-16522 PR_EXT:000005015 denotes Capn2
T12453 16476-16485 UBERON:0000922 denotes embryonic
T12452 16432-16439 SO_EXT:0001060 denotes isoform
T12451 16402-16415 GO:0009790 denotes embryogenesis
T12450 16359-16368 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12449 16218-16238 GO:0070527 denotes platelet aggregation
T12448 16218-16226 CL:0000233 denotes platelet
T12447 16164-16173 GO_MOP_EXT:catalysis denotes catalytic
T12446 16154-16181 PR_EXT:000005009 denotes μ-calpain catalytic subunit
T12445 16154-16163 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12444 16133-16137 NCBITaxon:10088 denotes mice
T12443 16131-16132 SO_EXT:sequence_nullness_or_absence denotes -
T12442 16129-16130 SO_EXT:sequence_nullness_or_absence denotes -
T12441 16124-16129 PR_EXT:000005009 denotes Capn1
T12440 16034-16041 SO_EXT:0001060 denotes isoform
T12439 15988-15997 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12438 15990-15997 GO_PR_EXT:calpain_1_complex denotes calpain
T12437 15981-15983 _FRAGMENT denotes μ-
T12436 15951-15960 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12435 15924-15933 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12434 15897-15905 SO_EXT:0001060 denotes isoforms
T12433 15866-15887 GO:0009790 denotes embryonic development
T12432 15866-15875 UBERON:0000922 denotes embryonic
T12431 15778-15787 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12430 15780-15787 GO_PR_EXT:calpain_1_complex denotes calpain
T12429 15771-15773 _FRAGMENT denotes μ-
T12428 15626-15630 NCBITaxon:10088 denotes mice
T12427 15611-15616 PR_EXT:000005022 denotes Capn4
T12426 15598-15607 GO_EXT:fatality_or_lethality denotes lethality
T12425 15588-15597 UBERON:0000922 denotes embryonic
T12424 15539-15548 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12423 15541-15548 GO_PR_EXT:calpain_1_complex denotes calpain
T12422 15532-15534 _FRAGMENT denotes μ-
T12421 15498-15508 GO:0065007 denotes regulatory
T12420 15479-15487 SO_EXT:sequence_coding_function denotes encoding
T12419 15473-15477 SO_EXT:0000704 denotes gene
T12418 15467-15472 PR_EXT:000005022 denotes Capn4
T12417 15422-15430 SO_EXT:0001060 denotes isoforms
T12416 15382-15393 GO_PATO_EXT:physiological_process_or_quality denotes physiologic
T12415 15329-15333 NCBITaxon:10088 denotes mice
T12414 15311-15315 SO_EXT:0000704 denotes Gene
T12413 15259-15268 GO:0065007 denotes regulated
T12412 15225-15233 SO_EXT:0001060 denotes isoforms
T12411 15104-15113 GO_EXT:0008233 denotes proteases
T12410 15051-15066 GO_EXT:negative_regulation denotes down-regulating
T12409 15016-15026 CHEBI_EXT:35222 denotes inhibitors
T12408 14938-14945 CHEBI_GO_SO_EXT:enzyme denotes enzymes
T7703 14792-14798 SO_EXT:sequence_cloned_entity denotes clones
T7702 14787-14791 CL_GO_EXT:cell denotes cell
T7701 14784-14791 CL:0002322 denotes ES cell
T7700 14738-14742 CL_GO_EXT:cell denotes cell
T7699 14696-14705 GO_PR_EXT:calpain_2_complex denotes m-calpain
T7698 14669-14673 CL_GO_EXT:cell denotes cell
T7697 14667-14679 UBERON:0019252 denotes 8-cell stage
T7696 14648-14655 UBERON:0000922 denotes embryos
T7695 14646-14647 SO_EXT:sequence_nullness_or_absence denotes -
T7694 14644-14645 SO_EXT:sequence_nullness_or_absence denotes -
T7693 14639-14644 PR_EXT:000005015 denotes Capn2
T7692 14601-14606 CL_GO_EXT:cell denotes cells
T7691 14598-14606 CL:0002322 denotes ES cells
T7690 14596-14597 SO_EXT:sequence_nullness_or_absence denotes -
T7689 14594-14595 SO_EXT:sequence_nullness_or_absence denotes -
T7688 14589-14594 PR_EXT:000005015 denotes Capn2
T7687 14556-14562 SO_EXT:sequence_cloned_entity denotes clones
T7686 14541-14545 CHEBI_EXT:23888 denotes drug
T7685 14503-14508 CL_GO_EXT:cell denotes cells
T7684 14500-14508 CL:0002322 denotes ES cells
T7683 14498-14499 SO_EXT:sequence_nullness_or_absence denotes -
T7682 14496-14497 SO_EXT:sequence_nullness_or_absence denotes -
T7681 14491-14496 PR_EXT:000005015 denotes Capn2
T6634 14111-14117 SO_EXT:0001023 denotes allele
T6633 14104-14110 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T6632 14093-14094 SO_EXT:sequence_nullness_or_absence denotes -
T6631 14091-14092 SO_EXT:normal_or_wild_type_or_present denotes +
T6630 14086-14091 PR_EXT:000005015 denotes Capn2
T6629 14073-14080 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T6628 14056-14061 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T6627 14016-14022 SO_EXT:0001023 denotes allele
T6626 14009-14015 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T6625 13998-13999 SO_EXT:sequence_nullness_or_absence denotes -
T6624 13996-13997 SO_EXT:normal_or_wild_type_or_present denotes +
T6623 13991-13996 PR_EXT:000005015 denotes Capn2
T6622 13978-13985 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T6621 13961-13966 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T6620 13888-13895 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T6619 13879-13884 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T6618 13865-13872 NCBITaxon:33208 denotes animals
T6617 13863-13864 SO_EXT:sequence_nullness_or_absence denotes -
T6616 13861-13862 SO_EXT:normal_or_wild_type_or_present denotes +
T6615 13856-13861 PR_EXT:000005015 denotes Capn2
T6614 13851-13852 SO_EXT:normal_or_wild_type_or_present denotes +
T6613 13849-13850 SO_EXT:normal_or_wild_type_or_present denotes +
T6612 13844-13849 PR_EXT:000005015 denotes Capn2
T6611 13736-13742 SO_EXT:0001023 denotes allele
T6607 13682-13687 PR_EXT:000005015 denotes Capn2
T6606 13638-13645 NCBITaxon:33208 denotes animals
T6605 13636-13637 SO_EXT:sequence_nullness_or_absence denotes -
T6604 13634-13635 SO_EXT:normal_or_wild_type_or_present denotes +
T6603 13629-13634 PR_EXT:000005015 denotes Capn2
T6602 13566-13573 NCBITaxon:33208 denotes animals
T6601 13564-13565 SO_EXT:sequence_nullness_or_absence denotes -
T6600 13562-13563 SO_EXT:normal_or_wild_type_or_present denotes +
T6599 13557-13562 PR_EXT:000005015 denotes Capn2
T6598 13551-13552 SO_EXT:normal_or_wild_type_or_present denotes +
T6597 13549-13550 SO_EXT:normal_or_wild_type_or_present denotes +
T6596 13544-13549 PR_EXT:000005015 denotes Capn2
T6595 13486-13494 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T6594 13457-13467 SO_EXT:transgenic_entity denotes transgenic
T6593 13451-13456 PR_EXT:000005022 denotes Capn4
T6592 13411-13418 NCBITaxon:33208 denotes animals
T6591 13331-13338 UBERON:0000922 denotes embryos
T6589 13297-13309 GO:0007566 denotes implantation
T6588 13259-13266 NCBITaxon:33208 denotes animals
T6587 13257-13258 SO_EXT:sequence_nullness_or_absence denotes -
T6586 13255-13256 SO_EXT:normal_or_wild_type_or_present denotes +
T6585 13250-13255 PR_EXT:000005015 denotes Capn2
T6584 13155-13159 NCBITaxon_UBERON_EXT:pup denotes pups
T6583 13037-13044 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T3038 46-54 CHEBI_SO_EXT:cysteine denotes cysteine
T3039 46-64 GO_EXT:0008234 denotes cysteine proteases
T3040 74-83 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3041 85-94 GO_PR_EXT:calpain_1_complex denotes calpain-1
T3042 100-109 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3043 111-119 PR_EXT:000005015 denotes capain-2
T3044 111-119 GO_PR_EXT:calpain_2_complex denotes capain-2
T3045 152-156 SO_EXT:0000704 denotes gene
T3046 178-183 SO_EXT:0000704 denotes genes
T3047 187-194 NCBITaxon:40674 denotes mammals
T3048 217-224 GO_EXT:dimer_macromolecular_complex denotes dimeric
T3049 225-232 CHEBI_GO_SO_EXT:enzyme denotes enzymes
T3050 263-272 GO_MOP_EXT:catalysis denotes catalytic
T3051 283-290 SO_EXT:sequence_coding_function denotes encoded
T3052 298-303 PR_EXT:000005009 denotes Capn1
T3053 305-311 PR_EXT:000005009 denotes μ-80 k
T3054 317-322 PR_EXT:000005015 denotes Capn2
T3055 324-330 PR_EXT:000005015 denotes m-80 k
T3056 332-337 SO_EXT:0000704 denotes genes
T3057 389-399 GO:0065007 denotes regulatory
T3058 408-415 SO_EXT:sequence_coding_function denotes encoded
T3059 423-428 PR_EXT:000005022 denotes Capn4
T3060 429-433 SO_EXT:0000704 denotes gene
T3061 439-445 _FRAGMENT denotes μ-80 k
T3062 457-465 PR_EXT:000005009 denotes subunits
T3063 450-465 PR_EXT:000005015 denotes m-80 k subunits
T3064 476-486 CHEBI_SO_EXT:amino_acid denotes amino acid
T3065 487-495 SO_EXT:biological_sequence denotes sequence
T3066 550-557 CHEBI_PR_EXT:protein denotes protein
T3067 650-660 GO:0010467 denotes expression
T3068 673-675 _FRAGMENT denotes μ-
T3069 682-689 GO_PR_EXT:calpain_1_complex denotes calpain
T3070 680-689 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3071 693-702 NCBITaxon:40674 denotes mammalian
T3072 703-710 UBERON:0000479 denotes tissues
T3073 734-741 SO_EXT:0001060 denotes isoform
T3074 826-830 CHEBI:29108 denotes Ca2+
T3075 882-886 CHEBI:29108 denotes Ca2+
T3076 891-900 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3077 917-921 CHEBI:29108 denotes Ca2+
T3078 926-935 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3079 966-968 _FRAGMENT denotes μ-
T3080 975-982 GO_PR_EXT:calpain_1_complex denotes calpain
T3081 973-982 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3082 1028-1035 CHEBI_EXT:calcium denotes calcium
T3083 1122-1133 GO:0005737 denotes cytoplasmic
T3084 1139-1143 CHEBI:29108 denotes Ca2+
T3085 1252-1262 GO:0065007 denotes regulation
T3086 1272-1279 CHEBI_GO_SO_EXT:enzyme denotes enzymes
T3087 1326-1336 GO:0065007 denotes regulating
T3088 1399-1409 CHEBI_GO_EXT:biological_process_or_quality_or_role denotes biological
T3089 1535-1541 UBERON_EXT:muscle_structure_or_tissue denotes muscle
T3090 1535-1541 _FRAGMENT denotes muscle
T3091 1550-1561 GO_EXT:muscle_structure_or_tissue_development denotes development
T3092 1542-1548 GO_EXT:biological_growth_entity_or_process denotes growth
T3093 1581-1589 CL:0000540 denotes neuronal
T3094 1590-1596 GO_EXT:biological_growth_entity_or_process denotes growth
T3095 1624-1628 CL_GO_EXT:cell denotes cell
T3096 1624-1634 GO:0007049 denotes cell cycle
T3097 1654-1669 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signal cascades
T3098 1683-1692 GO_PR_EXT:integrin denotes integrins
T3099 1697-1703 GO_EXT:biological_growth_entity_or_process denotes growth
T3100 1697-1711 GO_EXT:0008083 denotes growth factors
T3101 1717-1725 GO:0016020 denotes membrane
T3102 1760-1772 GO:0005856 denotes cytoskeleton
T3103 1777-1781 CL_GO_EXT:cell denotes cell
T3104 1777-1791 GO:0016477 denotes cell migration
T3105 1804-1828 GO:0010941 denotes regulation of cell death
T3106 1818-1822 CL_GO_EXT:cell denotes cell
T3107 1838-1846 GO_PATO_EXT:necrosis denotes necrosis
T3108 1851-1860 GO:0006915 denotes apoptosis
T3109 1986-1990 CL_GO_EXT:cell denotes cell
T3110 1986-1996 GO:0008219 denotes cell death
T3111 2050-2054 CL_GO_EXT:cell denotes cell
T3112 2065-2087 CHEBI_EXT:82824 denotes inhibitors of calpains
T3113 2199-2209 GO:0010467 denotes expression
T3114 2213-2224 PR_EXT:000005060 denotes calpastatin
T3115 2241-2248 CHEBI_PR_EXT:protein denotes protein
T3116 2249-2261 _FRAGMENT denotes inhibitor of
T3117 2271-2278 CHEBI_EXT:82824 denotes calpain
T3118 2262-2264 _FRAGMENT denotes μ-
T3119 2271-2278 GO_PR_EXT:calpain_1_complex denotes calpain
T3120 2269-2278 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3121 2353-2360 SO_EXT:0001060 denotes isoform
T3122 2416-2423 SO_EXT:0001060 denotes isoform
T3123 2459-2468 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3124 2472-2481 UBERON:0007376 denotes epidermal
T3125 2472-2495 PR_EXT:000006928 denotes epidermal growth factor
T3126 2482-2488 GO_EXT:biological_growth_entity_or_process denotes growth
T3127 2482-2495 GO_EXT:0008083 denotes growth factor
T3128 2497-2500 PR_EXT:000006928 denotes EGF
T3129 2510-2514 CL_GO_EXT:cell denotes cell
T3130 2510-2523 GO:0048870 denotes cell motility
T3131 2547-2556 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3132 2560-2590 PR_EXT:000006065 denotes interferon-inducible protein 9
T3133 2581-2588 CHEBI_PR_EXT:protein denotes protein
T3134 2599-2625 GO:0051546 denotes migration of keratinocytes
T3135 2612-2625 CL:0000312 denotes keratinocytes
T3136 2634-2638 CL_GO_EXT:cell denotes cell
T3137 2649-2666 CHEBI_EXT:82824 denotes calpain inhibitor
T3138 2696-2701 CHEBI:29917 denotes thiol
T3139 2696-2711 GO_EXT:0008234 denotes thiol-proteases
T3140 2745-2750 CL_GO_EXT:cell denotes cells
T3141 2759-2768 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3142 2791-2804 GO:0008283 denotes proliferation
T3143 2832-2841 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3144 2842-2852 GO:0010467 denotes expression
T3145 2872-2877 CL_GO_EXT:cell denotes cells
T3146 2916-2925 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3147 2930-2934 CL_GO_EXT:cell denotes cell
T3148 2959-2963 SO_EXT:0000704 denotes gene
T3149 2964-2972 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T3150 2976-2980 NCBITaxon:10088 denotes mice
T3151 3029-3042 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T3152 3052-3054 _FRAGMENT denotes μ-
T3153 3061-3068 GO_PR_EXT:calpain_1_complex denotes calpain
T3154 3059-3068 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3155 3107-3114 SO_EXT:0001060 denotes isoform
T3156 3160-3165 PR_EXT:000005022 denotes Capn4
T3157 3255-3257 _FRAGMENT denotes μ-
T3158 3264-3271 GO_PR_EXT:calpain_1_complex denotes calpain
T3159 3262-3271 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3160 3273-3278 PR_EXT:000005022 denotes Capn4
T3161 3278-3279 SO_EXT:sequence_nullness_or_absence denotes -
T3162 3280-3281 SO_EXT:sequence_nullness_or_absence denotes -
T3163 3282-3288 NCBITaxon:39107 denotes murine
T3164 3289-3296 UBERON:0000922 denotes embryos
T3165 3297-3301 GO:0016265 denotes died
T3166 3328-3337 GO:0007565 denotes gestation
T3167 3367-3369 _FRAGMENT denotes μ-
T3168 3375-3382 GO_PR_EXT:calpain_1_complex denotes calpain
T3169 3373-3382 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3170 3412-3419 UBERON:0000922 denotes embryos
T3171 3426-3431 PR_EXT:000005022 denotes Capn4
T3172 3431-3432 SO_EXT:sequence_nullness_or_absence denotes -
T3173 3433-3434 SO_EXT:sequence_nullness_or_absence denotes -
T3174 3435-3441 NCBITaxon:39107 denotes murine
T3175 3442-3451 UBERON:0000922 denotes embryonic
T3176 3452-3463 CL:0000057 denotes fibroblasts
T3177 3500-3507 UBERON:0000922 denotes embryos
T3178 3623-3631 GO_PR_EXT:spectrin denotes spectrin
T3179 3671-3680 GO_EXT:biological_movement_or_translocation_process denotes migration
T3180 3727-3734 GO_EXT:biological_release denotes release
T3181 3738-3753 GO:0005925 denotes focal adhesions
T3182 3744-3753 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesions
T3183 3784-3789 PR_EXT:000005022 denotes Capn4
T3184 3827-3835 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T3185 3843-3847 SO_EXT:0000704 denotes gene
T3186 3872-3881 UBERON:0000922 denotes embryonic
T3187 3882-3891 GO_EXT:fatality_or_lethality denotes lethality
T3188 3913-3925 GO:0007566 denotes implantation
T3189 3961-3970 UBERON:0000922 denotes embryonic
T3190 3971-3980 GO_EXT:fatality_or_lethality denotes lethality
T3191 4015-4020 PR_EXT:000005022 denotes Capn4
T3192 4020-4021 SO_EXT:sequence_nullness_or_absence denotes -
T3193 4022-4023 SO_EXT:sequence_nullness_or_absence denotes -
T3194 4024-4028 NCBITaxon:10088 denotes mice
T3195 4067-4075 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T3196 4165-4174 GO:0007565 denotes gestation
T3197 4202-4207 PR_EXT:000005022 denotes Capn4
T3198 4207-4208 SO_EXT:sequence_nullness_or_absence denotes -
T3199 4209-4210 SO_EXT:sequence_nullness_or_absence denotes -
T3200 4211-4215 NCBITaxon:10088 denotes mice
T3201 4240-4244 SO_EXT:sequence_nullness denotes null
T3202 4245-4253 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T3203 4269-4274 PR_EXT:000005009 denotes Capn1
T3204 4276-4284 SO_EXT:sequence_coding_function denotes encoding
T3205 4289-4298 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3206 4289-4316 PR_EXT:000005009 denotes μ-calpain catalytic subunit
T3207 4299-4308 GO_MOP_EXT:catalysis denotes catalytic
T3208 4373-4377 NCBITaxon:10088 denotes mice
T3209 4408-4417 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3210 4423-4432 CL:0000233 denotes platelets
T3211 4503-4508 PR_EXT:000005022 denotes Capn4
T3212 4509-4513 SO_EXT:sequence_nullness denotes null
T3213 4514-4518 NCBITaxon:10088 denotes mice
T3214 4519-4522 GO:0016265 denotes die
T3215 4530-4543 GO:0009790 denotes embryogenesis
T3216 4654-4659 PR_EXT:000005009 denotes Capn1
T3217 4670-4674 NCBITaxon:10088 denotes mice
T3218 4743-4752 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3219 4785-4798 GO:0009790 denotes embryogenesis
T3220 4954-4959 PR_EXT:000005015 denotes Capn2
T3221 4960-4964 SO_EXT:0000704 denotes gene
T3222 4965-4973 SO_EXT:sequence_coding_function denotes encoding
T3223 4978-4992 PR_EXT:000005015 denotes m-80 k subunit
T3224 4996-5000 NCBITaxon:10088 denotes mice
T3225 5022-5027 PR_EXT:000005015 denotes Capn2
T3226 5028-5032 SO_EXT:sequence_nullness denotes null
T3227 5033-5040 UBERON:0000922 denotes embryos
T3228 5041-5045 GO:0016265 denotes died
T3229 5059-5071 GO:0007566 denotes implantation
T3230 5095-5104 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3231 5132-5145 GO:0009790 denotes embryogenesis
T3232 5180-5189 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3233 5200-5209 GO:0010467 denotes expressed
T3234 5213-5222 UBERON:0000922 denotes embryonic
T3235 5213-5227 _FRAGMENT denotes embryonic stem
T3236 5233-5238 CL:0002322 denotes cells
T3237 5229-5231 _FRAGMENT denotes ES
T3238 5233-5238 CL_GO_EXT:cell denotes cells
T3239 5290-5299 GO:0007565 denotes gestation
T3240 5338-5347 GO_PR_EXT:calpain_2_complex denotes m-calpain
T3241 5352-5361 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T3242 5376-5389 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T3243 5409-5422 GO:0009790 denotes embryogenesis
T4774 5468-5473 PR_EXT:000005015 denotes Capn2
T4775 5483-5490 CL:0002322 denotes ES cell
T4776 5486-5490 CL_GO_EXT:cell denotes cell
T4777 5491-5497 SO_EXT:sequence_cloned_entity denotes clones
T4778 5514-5519 PR_EXT:000005015 denotes Capn2
T4779 5519-5520 SO_EXT:normal_or_wild_type_or_present denotes +
T4780 5521-5522 SO_EXT:sequence_nullness_or_absence denotes -
T4781 5523-5530 CL:0002322 denotes ES cell
T4782 5526-5530 CL_GO_EXT:cell denotes cell
T4783 5599-5603 CHEBI_EXT:23888 denotes drug
T4784 5614-5620 SO_EXT:sequence_cloned_entity denotes clones
T4785 5647-5652 PR_EXT:000005015 denotes Capn2
T4786 5697-5710 GO:0097617 denotes hybridization
T4787 5731-5736 CHEBI_SO_EXT:molecular_probe denotes probe
T4788 5764-5772 SO_EXT:sequence_upstreamness denotes upstream
T4789 5790-5800 GO:0097617 denotes hybridized
T4790 5811-5812 CHEBI_SO_EXT:base denotes b
T4791 5813-5818 PR_EXT:P23940 denotes BamHI
T4792 5835-5844 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4793 5845-5851 SO_EXT:0001023 denotes allele
T4794 5861-5862 CHEBI_SO_EXT:base denotes b
T4795 5863-5868 PR_EXT:P23940 denotes BamHI
T4796 5885-5891 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4797 5892-5898 SO_EXT:0001023 denotes allele
T4798 5917-5924 SO_EXT:0001026 denotes genomic
T4799 5952-5957 CHEBI_SO_EXT:molecular_probe denotes probe
T4800 5990-5991 CHEBI_SO_EXT:base denotes b
T4801 5992-6001 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4802 6011-6012 CHEBI_SO_EXT:base denotes b
T4803 6013-6019 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4804 6042-6043 CHEBI_SO_EXT:base denotes b
T4805 6044-6053 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4806 6063-6064 CHEBI_SO_EXT:base denotes b
T4807 6065-6071 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4808 6104-6105 CHEBI_SO_EXT:base denotes b
T4809 6106-6115 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4810 6125-6126 CHEBI_SO_EXT:base denotes b
T4811 6127-6133 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4812 6170-6175 CHEBI_SO_EXT:molecular_probe denotes probe
T4813 6193-6196 GO_EXT:phosphoglycerate_kinase denotes PGK
T4814 6197-6200 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T4815 6201-6209 SO_EXT:0005853 denotes cassette
T4816 6235-6236 CHEBI_SO_EXT:base denotes b
T4817 6237-6242 PR_EXT:P23940 denotes BamHI
T4818 6255-6260 PR_EXT:000005015 denotes Capn2
T4819 6260-6261 SO_EXT:normal_or_wild_type_or_present denotes +
T4820 6262-6263 SO_EXT:sequence_nullness_or_absence denotes -
T4821 6264-6272 CL:0002322 denotes ES cells
T4822 6267-6272 CL_GO_EXT:cell denotes cells
T4823 6294-6310 SO_EXT:0001644 denotes targeting vector
T4824 6340-6345 PR_EXT:000005015 denotes Capn2
T4825 6426-6435 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4826 6453-6455 SO_EXT:0000028 denotes bp
T4827 6468-6470 SO_EXT:0000028 denotes bp
T4828 6488-6494 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4829 6495-6501 SO_EXT:0001023 denotes allele
T4830 6513-6515 SO_EXT:0000028 denotes bp
T4831 6561-6565 CL_GO_EXT:cell denotes cell
T6467 7903-7907 NCBITaxon:10088 denotes mice
T6468 7941-7947 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T6469 7948-7953 PR_EXT:000005015 denotes Capn2
T6470 7954-7960 SO_EXT:0001023 denotes allele
T6471 7976-7980 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T6472 7981-7985 NCBITaxon:10088 denotes mice
T6473 8003-8009 UBERON:0000085 denotes morula
T6474 8044-8049 PR_EXT:000005015 denotes Capn2
T6475 8049-8050 SO_EXT:normal_or_wild_type_or_present denotes +
T6476 8051-8052 SO_EXT:sequence_nullness_or_absence denotes -
T6477 8058-8062 CL_GO_EXT:cell denotes cell
T6478 8082-8087 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T6479 8104-8109 PR_EXT:000005015 denotes Capn2
T6480 8111-8112 SO_EXT:sequence_nullness_or_absence denotes -
T6481 8118-8126 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T6482 8185-8190 PR_EXT:000005015 denotes Capn2
T6483 8190-8191 SO_EXT:normal_or_wild_type_or_present denotes +
T6484 8192-8193 SO_EXT:sequence_nullness_or_absence denotes -
T6485 8194-8201 NCBITaxon:33208 denotes animals
T6486 8252-8259 UBERON:0001062 denotes anatomy
T6487 8261-8273 GO:0000003 denotes reproduction
T6488 8278-8287 UBERON:0000104 denotes life span
T6489 8345-8350 PR_EXT:000005015 denotes Capn2
T6490 8350-8351 SO_EXT:sequence_nullness_or_absence denotes -
T6491 8352-8353 SO_EXT:sequence_nullness_or_absence denotes -
T6492 8420-8429 UBERON:0012101 denotes perinatal
T6493 8424-8429 GO:0007567 denotes natal
T6494 8430-8435 GO:0016265 denotes death
T6495 8444-8449 PR_EXT:000005015 denotes Capn2
T6496 8449-8450 SO_EXT:sequence_nullness_or_absence denotes -
T6497 8451-8452 SO_EXT:sequence_nullness_or_absence denotes -
T6498 8499-8504 PR_EXT:000005015 denotes Capn2
T6499 8504-8505 SO_EXT:sequence_nullness_or_absence denotes -
T6500 8506-8507 SO_EXT:sequence_nullness_or_absence denotes -
T6501 8508-8515 NCBITaxon:33208 denotes animals
T6502 8516-8524 GO:0016265 denotes perished
T6503 8546-8555 UBERON:0000922 denotes embryonic
T6504 8546-8567 GO:0009790 denotes embryonic development
T6505 8599-8608 UBERON:0000922 denotes embryonic
T6506 8609-8614 GO:0016265 denotes death
T6507 8634-8646 GO:0007566 denotes implantation
T6508 8654-8661 UBERON:0000922 denotes embryos
T6509 8739-8744 PR_EXT:000005015 denotes Capn2
T6510 8744-8745 SO_EXT:sequence_nullness_or_absence denotes -
T6511 8746-8747 SO_EXT:sequence_nullness_or_absence denotes -
T6512 8748-8755 UBERON:0000922 denotes embryos
T6513 8786-8792 UBERON:0000922 denotes embryo
T6514 8853-8858 PR_EXT:000005015 denotes Capn2
T6515 8858-8859 SO_EXT:sequence_nullness_or_absence denotes -
T6516 8860-8861 SO_EXT:sequence_nullness_or_absence denotes -
T6517 8862-8869 UBERON:0000922 denotes embryos
T6518 8879-8884 GO:0016265 denotes dying
T6519 8894-8906 GO:0007566 denotes implantation
T6520 8908-8915 UBERON:0000922 denotes Embryos
T6521 8943-8951 UBERON:0000993 denotes oviducts
T6522 8955-8963 GO:0007565 denotes pregnant
T6523 8964-8971 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T6524 9086-9098 GO:0007566 denotes implantation
T6525 9099-9106 UBERON:0000922 denotes embryos
T6526 9112-9117 PR_EXT:000005015 denotes Capn2
T6527 9117-9118 SO_EXT:sequence_nullness_or_absence denotes -
T6528 9119-9120 SO_EXT:sequence_nullness_or_absence denotes -
T6529 9157-9162 PR_EXT:000005015 denotes Capn2
T6530 9162-9163 SO_EXT:sequence_nullness_or_absence denotes -
T6531 9164-9165 SO_EXT:sequence_nullness_or_absence denotes -
T6532 9166-9173 UBERON:0000922 denotes embryos
T6533 9195-9207 UBERON:0019252 denotes 8-cell stage
T6534 9197-9201 CL_GO_EXT:cell denotes cell
T6535 9301-9311 UBERON:0000358 denotes blastocyst
T6536 9319-9326 UBERON:0000922 denotes embryos
T6537 9332-9337 PR_EXT:000005015 denotes Capn2
T6538 9337-9338 SO_EXT:sequence_nullness_or_absence denotes -
T6539 9339-9340 SO_EXT:sequence_nullness_or_absence denotes -
T6540 9358-9363 PR_EXT:000005015 denotes Capn2
T6590 13318-13330 GO:0007566 denotes implantation
T7680 14453-14458 CL_GO_EXT:cell denotes cells
T7679 14450-14458 CL:0002322 denotes ES cells
T7678 14448-14449 SO_EXT:sequence_nullness_or_absence denotes -
T7677 14446-14447 SO_EXT:normal_or_wild_type_or_present denotes +
T7676 14441-14446 PR_EXT:000005022 denotes Capn4
T7675 14395-14400 CL_GO_EXT:cell denotes cells
T7674 14392-14400 CL:0002322 denotes ES cells
T7673 14319-14334 GO:0035822 denotes gene conversion
T7672 14319-14323 SO_EXT:0000704 denotes gene
T7671 14310-14315 CL_GO_EXT:cell denotes cells
T7670 14303-14309 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T7669 14263-14267 CHEBI:42768 denotes G418
T7668 14219-14225 SO_EXT:sequence_cloned_entity denotes clonal
T7667 14195-14200 CL_GO_EXT:cell denotes cells
T7666 14192-14200 CL:0002322 denotes ES cells
T7665 14190-14191 SO_EXT:sequence_nullness_or_absence denotes -
T7664 14188-14189 SO_EXT:normal_or_wild_type_or_present denotes +
T7663 14183-14188 PR_EXT:000005015 denotes Capn2
T7662 14177-14182 CL_GO_EXT:cell denotes cells
T7661 14174-14182 CL:0002322 denotes ES cells
T7660 14172-14173 SO_EXT:sequence_nullness_or_absence denotes -
T7659 14170-14171 SO_EXT:sequence_nullness_or_absence denotes -
T7658 14165-14170 PR_EXT:000005015 denotes Capn2
T7657 14177-14182 GO:0048468 denotes cells
T12549 19777-19782 CL_GO_EXT:cell denotes cells
T15429 27876-27880 SO_EXT:0000704 denotes gene
T15428 27797-27801 NCBITaxon:10088 denotes mice
T15427 27791-27796 UBERON:0007023 denotes adult
T15426 27770-27783 GO:0009790 denotes embryogenesis
T15425 27757-27769 GO:0007566 denotes implantation
T15424 27735-27744 GO_PR_EXT:calpain_2_complex denotes m-calpain
T15423 27667-27675 SO_EXT:0001060 denotes isoforms
T15422 27629-27638 GO_PR_EXT:calpain_2_complex denotes m-calpain
T15421 27631-27638 GO_PR_EXT:calpain_1_complex denotes calpain
T15420 27622-27624 _FRAGMENT denotes μ-
T15419 27566-27575 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T15418 27488-27500 GO:0007566 denotes implantation
T15417 27449-27462 GO:0009790 denotes embryogenesis
T15416 27442-27448 NCBITaxon:39107 denotes murine
T15415 27401-27410 GO_PR_EXT:calpain_2_complex denotes m-calpain
T15414 27358-27367 GO_PR_EXT:calpain_2_complex denotes m-calpain
T15413 27360-27367 GO_PR_EXT:calpain_1_complex denotes calpain
T15412 27351-27353 _FRAGMENT denotes μ-
T15411 27341-27349 SO_EXT:0001060 denotes isoforms
T15410 27291-27304 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T15985 28156-28170 PR_EXT:000005015 denotes m-80 k subunit
T15984 28150-28155 NCBITaxon:10088 denotes mouse
T15983 28124-28132 SO_EXT:sequence_coding_function denotes encoding
T15982 28113-28123 SO_EXT:0000317 denotes cDNA clone
T15981 28097-28107 GO_SO_EXT:chromosome denotes chromosome
T15980 28092-28093 CHEBI_SO_EXT:base denotes b
T15979 28067-28072 SO_EXT:0000147 denotes exons
T15978 28040-28046 PR_EXT:000005015 denotes m-80 k
T15977 28015-28038 PR_EXT:000005015 denotes m-calpain large subunit
T15976 28015-28024 GO_PR_EXT:calpain_2_complex denotes m-calpain
T15975 27996-28007 CHEBI_SO_EXT:amino_acid denotes amino acids
T15974 27980-27987 SO_EXT:sequence_coding_function denotes encodes
T15973 27975-27979 SO_EXT:0000704 denotes gene
T15972 27969-27974 PR_EXT:000005015 denotes Capn2
T15971 27963-27968 NCBITaxon:10088 denotes mouse
T15970 27947-27952 PR_EXT:000005015 denotes Capn2
T15969 27941-27946 NCBITaxon:10088 denotes mouse
T15968 27911-27918 SO_EXT:sequence_cloning_process denotes Cloning
T16695 29784-29790 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T16694 29770-29779 SO_EXT:wild_type_entity_or_quality denotes wild-type
T16693 29720-29728 SO_EXT:0005853 denotes cassette
T16692 29716-29719 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T16691 29712-29715 GO_EXT:phosphoglycerate_kinase denotes PGK
T16690 29680-29685 PR_EXT:P23940 denotes BamHI
T16689 29633-29639 SO_EXT:0001023 denotes allele
T16688 29626-29632 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T16687 29608-29613 PR_EXT:P23940 denotes BamHI
T16686 29534-29539 PR_EXT:P23940 denotes BamHI
T16685 29521-29528 SO_EXT:sequence_cloning_process denotes cloning
T16684 29499-29507 SO_EXT:0005853 denotes cassette
T16683 29495-29498 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T16682 29491-29494 GO_EXT:phosphoglycerate_kinase denotes PGK
T16681 29475-29483 SO_EXT:sequence_upstreamness denotes upstream
T16680 29468-29474 SO_EXT:0000440 denotes vector
T16678 29420-29425 SO_EXT:0000147 denotes exons
T17436 31443-31458 CHEBI:50144 denotes sodium pyruvate
T17435 31427-31436 CHEBI_SO_EXT:glutamine denotes glutamine
T17434 31409-31420 CHEBI_SO_EXT:amino_acid denotes amino acids
T17433 31381-31386 UBERON:0001977 denotes serum
T17432 31374-31380 NCBITaxon:27592 denotes bovine
T17431 31337-31344 CHEBI:17234 denotes glucose
T17430 31313-31317 CL_GO_EXT:cell denotes cell
T17429 31310-31317 CL:0002322 denotes ES cell
T17428 31303-31306 CHEBI:16526 denotes CO2
T17427 31274-31285 CL:0000057 denotes fibroblasts
T17426 31264-31273 UBERON:0000922 denotes embryonic
T17425 31258-31263 NCBITaxon:10088 denotes mouse
T17424 31214-31221 CHEBI:5291 denotes gelatin
T17423 31184-31189 CL_GO_EXT:cell denotes cells
T17422 31181-31189 CL:0002322 denotes ES cells
T17421 31165-31171 SO_EXT:sequence_cloned_entity denotes clones
T17420 31126-31138 GO:0009294 denotes transfection
T17419 31112-31116 CL_GO_EXT:cell denotes cell
T17418 31109-31116 CL:0002322 denotes ES cell
T21093 6624-6631 CL:0002322 denotes ES cell
T21094 6627-6631 CL_GO_EXT:cell denotes cell
T21095 6670-6675 PR_EXT:000005015 denotes Capn2
T21096 6755-6760 PR_EXT:P23940 denotes BamHI
T21097 6770-6781 SO_EXT:genomic_DNA denotes genomic DNA
T21098 6778-6781 CHEBI_SO_EXT:DNA denotes DNA
T21099 6797-6805 CL:0002322 denotes ES cells
T21100 6800-6805 CL_GO_EXT:cell denotes cells
T21101 6810-6820 GO:0097617 denotes hybridized
T21102 6828-6831 CHEBI:42098 denotes DIG
T21103 6832-6839 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T21104 6844-6846 SO_EXT:0000028 denotes bp
T21105 6847-6852 PR_EXT:P23940 denotes BamHI
T21106 6853-6860 PR_EXT:P43870 denotes HindIII
T21107 6890-6898 SO_EXT:sequence_upstreamness denotes upstream
T21108 6923-6939 SO_EXT:0001644 denotes targeting vector
T21109 6959-6960 CHEBI_SO_EXT:base denotes b
T21110 6961-6966 PR_EXT:P23940 denotes BamHI
T21111 6997-7006 SO_EXT:wild_type_entity_or_quality denotes wild-type
T21112 7007-7013 SO_EXT:0001023 denotes allele
T21113 7033-7038 CL_GO_EXT:cell denotes cells
T21114 7055-7056 CHEBI_SO_EXT:base denotes b
T21115 7075-7081 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T21116 7082-7088 SO_EXT:0001023 denotes allele
T21117 7118-7122 CL_GO_EXT:cell denotes cell
T21118 7254-7256 SO_EXT:0000028 denotes bp
T21119 7274-7283 SO_EXT:wild_type_entity_or_quality denotes wild-type
T21120 7284-7290 SO_EXT:0001023 denotes allele
T21121 7302-7304 SO_EXT:0000028 denotes bp
T21122 7322-7328 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T21123 7329-7335 SO_EXT:0001023 denotes allele
T21124 7372-7378 SO_EXT:0000112 denotes primer
T21125 7390-7396 SO_EXT:0000188 denotes intron
T21126 7429-7445 SO_EXT:0001644 denotes targeting vector
T21127 7460-7466 SO_EXT:0001023 denotes allele
T21128 7476-7485 SO:0000077 denotes antisense
T21129 7486-7493 SO_EXT:0000112 denotes primers
T21130 7522-7531 SO_EXT:wild_type_entity_or_quality denotes wild-type
T21131 7532-7538 SO_EXT:0001023 denotes allele
T21132 7547-7556 SO:0000077 denotes antisense
T21133 7557-7563 SO_EXT:0000112 denotes primer
T21134 7575-7579 SO_EXT:0000147 denotes exon
T21135 7613-7619 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T21136 7620-7628 SO_EXT:biological_sequence denotes sequence
T21137 7646-7655 SO:0000077 denotes antisense
T21138 7656-7662 SO_EXT:0000112 denotes primer
T21139 7670-7673 GO_EXT:phosphoglycerate_kinase denotes PGK
T21140 7674-7677 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T21141 7678-7686 SO_EXT:0005853 denotes cassette
T21142 7728-7737 SO_EXT:wild_type_entity_or_quality denotes wild-type
T21143 7738-7744 SO_EXT:0001023 denotes allele
T21144 7752-7757 CL_GO_EXT:cell denotes cells
T21145 7771-7777 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T21146 7778-7784 SO_EXT:0001023 denotes allele
T21147 7830-7836 SO_EXT:sequence_cloned_entity denotes clones
T21148 7839-7840 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes M
T21149 7854-7863 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T21150 7871-7877 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T22619 12251-12258 UBERON:0000922 denotes embryos
T22618 12241-12242 SO_EXT:sequence_nullness_or_absence denotes -
T22617 12239-12240 SO_EXT:normal_or_wild_type_or_present denotes +
T22616 12234-12239 PR_EXT:000005015 denotes Capn2
T22615 12202-12209 UBERON:0000922 denotes Embryos
T22614 12193-12200 UBERON:0000922 denotes embryos
T22613 12176-12186 UBERON:0000358 denotes blastocyst
T22612 12097-12099 SO_EXT:0000028 denotes bp
T22611 12081-12087 SO_EXT:0001023 denotes allele
T22610 12074-12080 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T22609 12063-12065 SO_EXT:0000028 denotes bp
T22608 12051-12057 SO_EXT:0001023 denotes allele
T22607 12041-12050 SO_EXT:wild_type_entity_or_quality denotes wild-type
T22606 12030-12032 SO_EXT:0000028 denotes bp
T22605 11993-12000 SO_EXT:0001023 denotes alleles
T22604 11952-11968 SO_EXT:0001644 denotes targeting vector
T22932 9708-9716 SO_EXT:genotype_or_entity_with_genotype denotes Genotype
T22933 9756-9761 PR_EXT:000005015 denotes Capn2
T22934 9762-9772 SO_EXT:transgenic_entity denotes transgenic
T22935 9773-9777 NCBITaxon:10088 denotes mice
T23672 34462-34467 PR_EXT:000005015 denotes Capn2
T6551 9650-9655 PR_EXT:000005015 denotes Capn2
T7656 14151-14164 _FRAGMENT denotes generation of
T15993 28425-28429 SO_EXT:cDNA denotes cDNA
T6541 9374-9381 UBERON:0000922 denotes embryos
T6542 9399-9411 UBERON:0019252 denotes 8-cell stage
T6543 9401-9405 CL_GO_EXT:cell denotes cell
T6544 9439-9448 GO_PR_EXT:calpain_2_complex denotes m-calpain
T6545 9463-9470 GO_EXT:fatality_or_lethality denotes fatally
T6546 9499-9512 UBERON:0019248 denotes early embryos
T6547 9586-9590 CHEBI_SO_EXT:mRNA denotes mRNA
T6548 9586-9601 CHEBI_SO_EXT:mRNA denotes mRNA transcript
T6549 9591-9601 SO_EXT:0000673 denotes transcript
T6550 9605-9612 CHEBI_PR_EXT:protein denotes protein
T6552 9655-9656 SO_EXT:sequence_nullness_or_absence denotes -
T6553 9657-9658 SO_EXT:sequence_nullness_or_absence denotes -
T6554 9659-9666 UBERON:0000922 denotes embryos
T6555 9685-9691 UBERON:0000085 denotes morula
T6582 13015-13020 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T6581 13001-13008 NCBITaxon:33208 denotes animals
T6580 12999-13000 SO_EXT:sequence_nullness_or_absence denotes -
T6579 12997-12998 SO_EXT:normal_or_wild_type_or_present denotes +
T6578 12992-12997 PR_EXT:000005015 denotes Capn2
T6577 12987-12988 SO_EXT:normal_or_wild_type_or_present denotes +
T6576 12985-12986 SO_EXT:normal_or_wild_type_or_present denotes +
T6575 12980-12985 PR_EXT:000005015 denotes Capn2
T6574 12944-12951 NCBITaxon:33208 denotes animals
T6573 12942-12943 SO_EXT:sequence_nullness_or_absence denotes -
T6572 12940-12941 SO_EXT:normal_or_wild_type_or_present denotes +
T6571 12935-12940 PR_EXT:000005015 denotes Capn2
T6570 12909-12910 SO_EXT:normal_or_wild_type_or_present denotes +
T6569 12907-12908 SO_EXT:normal_or_wild_type_or_present denotes +
T6568 12902-12907 PR_EXT:000005015 denotes Capn2
T6567 12876-12884 GO_EXT:breeding denotes breeding
T6566 12842-12849 NCBITaxon:33208 denotes animals
T6565 12715-12722 NCBITaxon:33208 denotes animals
T6564 12714-12715 SO_EXT:sequence_nullness_or_absence denotes -
T6563 12712-12713 SO_EXT:normal_or_wild_type_or_present denotes +
T6562 12707-12712 PR_EXT:000005015 denotes Capn2
T6561 12702-12703 SO_EXT:normal_or_wild_type_or_present denotes +
T6560 12700-12701 SO_EXT:normal_or_wild_type_or_present denotes +
T6559 12695-12700 PR_EXT:000005015 denotes Capn2
T6558 12641-12648 UBERON:0000922 denotes embryos
T6557 12628-12640 GO:0007566 denotes implantation
T6556 12606-12618 GO:0007566 denotes implantation
T6610 13729-13735 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T6609 13689-13690 SO_EXT:sequence_nullness_or_absence denotes -
T6608 13687-13688 SO_EXT:normal_or_wild_type_or_present denotes +
T3037 30-34 CHEBI:29108 denotes Ca2+
T12815 27126-27136 GO:0010467 denotes expression
T12814 27013-27017 SO_EXT:sequence_nullness denotes null
T12813 26955-26964 SO_EXT:wild_type_entity_or_quality denotes wild-type
R274 T3062 T3061 _lexicallyChainedTo subunits,μ-80 k
R275 T3069 T3068 _lexicallyChainedTo calpain,μ-
R277 T3153 T3152 _lexicallyChainedTo calpain,μ-
R278 T3080 T3079 _lexicallyChainedTo calpain,μ-
R279 T3091 T3090 _lexicallyChainedTo development,muscle
R280 T3158 T3157 _lexicallyChainedTo calpain,μ-
R281 T3168 T3167 _lexicallyChainedTo calpain,μ-
R283 T3117 T3116 _lexicallyChainedTo calpain,inhibitor of
R284 T3119 T3118 _lexicallyChainedTo calpain,μ-
R285 T3236 T3235 _lexicallyChainedTo cells,embryonic stem
R286 T3236 T3237 _lexicallyChainedTo cells,ES
R2220 T7657 T7656 _lexicallyChainedTo cells,generation of
R2358 T12423 T12422 _lexicallyChainedTo calpain,μ-
R2361 T12430 T12429 _lexicallyChainedTo calpain,μ-
R2362 T12438 T12437 _lexicallyChainedTo calpain,μ-
R2363 T12558 T12557 _lexicallyChainedTo calpain,μ-
R2365 T12570 T12569 _lexicallyChainedTo calpain,μ-
R2366 T12589 T12588 _lexicallyChainedTo proteins,phosphorylation of
R2367 T12594 T12593 _lexicallyChainedTo calpain,μ-
R2368 T12755 T12754 _lexicallyChainedTo cells,ES
R2369 T12682 T12681 _lexicallyChainedTo during meiosis,chromosome segregation
R2371 T12696 T12695 _lexicallyChainedTo calpain,μ-
R2372 T12723 T12722 _lexicallyChainedTo calpain,μ-
R4587 T15413 T15412 _lexicallyChainedTo calpain,μ-
R4588 T15421 T15420 _lexicallyChainedTo calpain,μ-