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Id Subject Object Predicate Lexical cue
T15137 12147-12148 NN denotes %
T15136 12143-12147 CD denotes 22.5
T15135 12141-12143 , denotes ,
T15134 12124-12133 VBN denotes genotyped
T15133 12134-12141 NNS denotes animals
T15132 12121-12123 CD denotes 80
T15131 12118-12120 IN denotes of
T15130 12112-12117 NN denotes total
T15129 12110-12111 DT denotes a
T15128 12107-12109 IN denotes of
T15127 12149-12153 VBD denotes were
T15126 12103-12106 IN denotes Out
T15125 12102-12239 sentence denotes Out of a total of 80 genotyped animals, 22.5% were wild-type and 77.5% were heterozygous, with no homozygous null progeny observed [32].
T15124 12101-12102 . denotes .
T15123 12080-12092 JJ denotes heterozygous
T15122 12093-12101 NN denotes genotype
T15121 12076-12079 DT denotes the
T15120 12073-12075 IN denotes of
T15119 12067-12072 NN denotes favor
T15118 12064-12066 IN denotes in
T15117 12054-12056 , denotes ,
T15116 12042-12046 RBR denotes less
T15115 12039-12041 IN denotes if
T15114 12047-12054 JJ denotes extreme
T15113 12037-12039 , denotes ,
T15112 12030-12037 JJ denotes similar
T15111 12056-12063 VBG denotes skewing
T15110 12028-12029 DT denotes a
T15109 12023-12027 IN denotes with
T15108 12015-12022 NN denotes progeny
T15107 11996-12006 NNS denotes colleagues
T15106 11992-11995 CC denotes and
T15105 11982-11991 NNP denotes Zimmerman
T15104 11979-11981 IN denotes by
T15103 11969-11978 VBN denotes generated
T15102 11953-11963 JJ denotes transgenic
T15101 11947-11952 NN denotes Capn4
T15100 11964-11968 NN denotes line
T15099 11943-11946 DT denotes the
T15098 11933-11942 VBG denotes involving
T15097 11925-11932 NNS denotes crosses
T15096 11912-11924 JJ denotes heterozygous
T15095 11910-11912 , denotes ,
T15094 12007-12014 VBD denotes yielded
T15093 11897-11910 RB denotes Interestingly
T15092 11896-12102 sentence denotes Interestingly, heterozygous crosses involving the Capn4 transgenic line generated by Zimmerman and colleagues yielded progeny with a similar, if less extreme, skewing in favor of the heterozygous genotype.
T15091 11895-11896 . denotes .
T15090 11886-11895 NN denotes offspring
T15089 11873-11885 JJ denotes heterozygous
T15088 11870-11872 IN denotes of
T15087 11850-11858 VBN denotes expected
T15086 11845-11849 IN denotes than
T15085 11837-11844 JJR denotes greater
T15084 11859-11869 NN denotes proportion
T15083 11835-11836 DT denotes a
T15082 11830-11834 IN denotes with
T15081 11822-11829 NN denotes progeny
T15080 11808-11812 RB denotes also
T15079 11790-11802 JJ denotes heterozygous
T15078 11786-11789 CC denotes and
T15077 11803-11807 NNS denotes mice
T15076 11780-11781 HYPH denotes -
T15075 11781-11785 NN denotes type
T15074 11776-11780 JJ denotes wild
T15073 11768-11775 IN denotes between
T15072 11813-11821 VBD denotes produced
T15071 11760-11767 NNS denotes Crosses
T15070 11759-11896 sentence denotes Crosses between wild-type and heterozygous mice also produced progeny with a greater than expected proportion of heterozygous offspring.
T15069 11758-11759 . denotes .
T15068 11752-11758 NN denotes result
T15067 11747-11751 DT denotes this
T15066 11743-11746 IN denotes for
T15065 11723-11730 JJ denotes obvious
T15064 11731-11742 NN denotes explanation
T15063 11720-11722 DT denotes no
T15062 11711-11716 EX denotes there
T15061 11709-11711 , denotes ,
T15060 11702-11709 JJ denotes present
T15059 11717-11719 VBZ denotes is
T15058 11699-11701 IN denotes At
T15057 11698-11759 sentence denotes At present, there is no obvious explanation for this result.
T15056 11697-11698 . denotes .
T15055 11688-11697 NNS denotes weanlings
T15054 11685-11687 IN denotes of
T15053 11683-11684 -RRB- denotes )
T15052 11678-11682 CD denotes 88.4
T15051 11682-11683 NN denotes %
T15050 11677-11678 -LRB- denotes (
T15049 11675-11676 SYM denotes -
T15048 11674-11675 HYPH denotes /
T15047 11673-11674 SYM denotes +
T15046 11668-11673 NN denotes Capn2
T15045 11665-11667 IN denotes to
T15044 11663-11664 -RRB- denotes )
T15043 11658-11662 CD denotes 11.6
T15042 11662-11663 NN denotes %
T15041 11657-11658 -LRB- denotes (
T15040 11655-11656 SYM denotes +
T15039 11654-11655 HYPH denotes /
T15038 11653-11654 SYM denotes +
T15037 11648-11653 NN denotes Capn2
T15036 11645-11647 IN denotes of
T15035 11625-11638 JJ denotes non-Mendelian
T15034 11618-11624 RB denotes highly
T15033 11639-11644 NN denotes ratio
T15032 11614-11617 DT denotes the
T15031 11596-11609 NN denotes interbreeding
T15030 11583-11595 JJ denotes heterozygous
T15029 11578-11582 IN denotes from
T15028 11570-11577 NN denotes progeny
T15027 11567-11569 IN denotes of
T15026 11556-11566 NN denotes genotyping
T15025 11552-11555 DT denotes the
T15024 11545-11551 IN denotes during
T15023 11539-11544 VBD denotes arose
T15022 11533-11538 WDT denotes which
T15021 11610-11613 VBD denotes was
T15020 11523-11532 NN denotes curiosity
T15019 11519-11522 CD denotes One
T15018 11518-11698 sentence denotes One curiosity which arose during the genotyping of progeny from heterozygous interbreeding was the highly non-Mendelian ratio of Capn2+/+ (11.6%) to Capn2+/- (88.4%) of weanlings.
T15017 11517-11518 . denotes .
T15016 11509-11517 NNS denotes attempts
T15015 11503-11508 DT denotes these
T15014 11490-11493 RB denotes far
T15013 11485-11489 RB denotes thus
T15012 11481-11484 VBZ denotes has
T15011 11464-11470 NN denotes rescue
T15010 11471-11480 NN denotes transgene
T15009 11460-11463 DT denotes the
T15008 11457-11459 IN denotes of
T15007 11446-11456 NN denotes expression
T15006 11439-11445 JJ denotes stable
T15005 11429-11438 VBG denotes achieving
T15004 11426-11428 IN denotes in
T15003 11415-11425 NN denotes difficulty
T15002 11413-11415 , denotes ,
T15001 11494-11502 VBN denotes hampered
T15000 11406-11413 RB denotes However
T14999 11405-11518 sentence denotes However, difficulty in achieving stable expression of the rescue transgene has thus far hampered these attempts.
T14998 11404-11405 . denotes .
T14997 11397-11399 VBZ denotes is
T14996 11384-11385 HYPH denotes -
T14995 11385-11389 NN denotes type
T14994 11380-11384 JJ denotes wild
T14993 11369-11379 JJ denotes endogenous
T14992 11359-11368 VBG denotes remaining
T14991 11390-11396 NN denotes allele
T14990 11355-11358 DT denotes the
T14989 11400-11404 VBN denotes lost
T14988 11348-11354 IN denotes before
T14987 11339-11347 NN denotes activity
T14986 11330-11331 HYPH denotes -
T14985 11331-11338 NN denotes calpain
T14984 11329-11330 NN denotes m
T14983 11309-11321 RB denotes preemptively
T14982 11322-11328 VB denotes rescue
T14981 11306-11308 TO denotes to
T14980 11300-11305 NN denotes order
T14979 11297-11299 IN denotes in
T14978 11286-11296 NN denotes conversion
T14977 11281-11285 NN denotes gene
T14976 11278-11280 IN denotes to
T14975 11272-11277 JJ denotes prior
T14974 11255-11261 NN denotes rescue
T14973 11250-11254 NN denotes cDNA
T14972 11244-11249 NN denotes Capn2
T14971 11262-11271 NN denotes transgene
T14970 11242-11243 DT denotes a
T14969 11237-11241 IN denotes from
T14968 11227-11228 NN denotes k
T14967 11224-11226 CD denotes 80
T14966 11223-11224 HYPH denotes -
T14965 11222-11223 NN denotes m
T14964 11229-11236 NN denotes subunit
T14963 11218-11221 DT denotes the
T14962 11210-11217 VB denotes express
T14961 11207-11209 TO denotes to
T14960 11203-11206 RB denotes way
T14959 11197-11202 RB denotes under
T14958 11193-11196 VBP denotes are
T14957 11185-11192 NNS denotes Efforts
T14956 11184-11405 sentence denotes Efforts are under way to express the m-80 k subunit from a Capn2 cDNA rescue transgene prior to gene conversion in order to preemptively rescue m-calpain activity before the remaining endogenous wild-type allele is lost.
T14955 11183-11184 . denotes .
T14954 11178-11183 NNS denotes cells
T14953 11175-11177 NN denotes ES
T14952 11172-11174 IN denotes of
T14951 11162-11171 NN denotes selection
T14950 11155-11161 JJ denotes clonal
T14949 11152-11154 CC denotes or
T14948 11142-11151 NN denotes viability
T14947 11137-11141 NN denotes cell
T14946 11117-11124 RB denotes somehow
T14945 11104-11110 NN denotes mutant
T14885 10791-10939 sentence denotes Furthermore, calpain-independent mechanisms for protecting cell viability might exist in these cell lines that are absent in early embryonic cells.
T14884 10790-10791 . denotes .
T14883 10785-10790 NNS denotes cells
T14882 10781-10784 DT denotes the
T14881 10778-10780 IN denotes of
T14880 10768-10777 NN denotes viability
T14879 10764-10767 DT denotes the
T14878 10752-10763 VBG denotes maintaining
T14877 10748-10751 IN denotes for
T14876 10737-10747 JJ denotes sufficient
T14875 10734-10736 VBZ denotes is
T14874 10731-10733 PRP denotes it
T14873 10727-10730 CC denotes and
T14872 10725-10727 , denotes ,
T14871 10713-10718 NN denotes Capn4
T14870 10706-10712 NN denotes mutant
T14869 10719-10725 NN denotes allele
T14868 10701-10705 DT denotes this
T14867 10696-10700 IN denotes from
T14866 10684-10686 VBZ denotes is
T14865 10682-10684 , denotes ,
T14864 10675-10682 NNS denotes methods
T14863 10669-10674 DT denotes these
T14862 10666-10668 IN denotes by
T14861 10655-10665 JJ denotes detectable
T14860 10648-10654 NNS denotes levels
T14859 10640-10647 IN denotes beneath
T14858 10638-10640 , denotes ,
T14857 10630-10638 NN denotes activity
T14856 10622-10629 NN denotes calpain
T14855 10619-10621 IN denotes of
T14854 10606-10611 NN denotes trace
T14853 10612-10618 NN denotes amount
T14852 10604-10605 DT denotes a
T14851 10687-10695 VBN denotes retained
T14850 10599-10603 IN denotes that
T14849 10597-10599 , denotes ,
T14848 10590-10597 RBR denotes earlier
T14847 10580-10589 VBN denotes discussed
T14846 10577-10579 IN denotes as
T14845 10575-10577 , denotes ,
T14844 10564-10575 JJ denotes conceivable
T14843 10561-10563 VBZ denotes is
T14842 10558-10560 PRP denotes It
T14841 10557-10791 sentence denotes It is conceivable, as discussed earlier, that a trace amount of calpain activity, beneath levels detectable by these methods, is retained from this mutant Capn4 allele, and it is sufficient for maintaining the viability of the cells.
T14840 10556-10557 . denotes .
T14839 10538-10547 NN denotes breakdown
T14838 10529-10537 NN denotes spectrin
T14837 10548-10556 NNS denotes products
T14836 10514-10528 JJ denotes characteristic
T14835 10511-10513 IN denotes of
T14834 10500-10510 NN denotes appearance
T14833 10496-10499 DT denotes the
T14832 10493-10495 IN denotes by
T14831 10490-10492 CC denotes or
T14830 10479-10489 NN denotes zymography
T14829 10472-10478 NN denotes casein
T14828 10469-10471 IN denotes by
T14827 10460-10468 VBN denotes assessed
T14826 10457-10459 IN denotes as
T14825 10455-10457 , denotes ,
T14824 10447-10455 NN denotes activity
T14823 10439-10446 NN denotes calpain
T14822 10436-10438 IN denotes of
T14821 10422-10430 JJ denotes apparent
T14820 10431-10435 NN denotes lack
T14819 10419-10421 DT denotes an
T14818 10411-10418 IN denotes despite
T14817 10403-10410 NN denotes culture
T14816 10400-10402 IN denotes in
T14815 10386-10388 VB denotes be
T14814 10382-10385 MD denotes can
T14813 10363-10374 JJ denotes hypomorphic
T14812 10351-10362 JJ denotes presumptive
T14811 10375-10381 NN denotes allele
T14810 10347-10350 DT denotes the
T14809 10342-10346 IN denotes from
T14808 10332-10335 NN denotes MEF
T14807 10328-10331 CC denotes and
T14806 10325-10327 NN denotes ES
T14805 10323-10324 SYM denotes -
T14804 10322-10323 HYPH denotes /
T14803 10321-10322 SYM denotes -
T14802 10336-10341 NNS denotes cells
T14801 10316-10321 NN denotes Capn4
T14800 10389-10399 VBN denotes maintained
T14799 10311-10315 IN denotes that
T14798 10299-10301 VB denotes be
T14797 10294-10298 RB denotes also
T14796 10287-10293 MD denotes should
T14795 10302-10310 VBN denotes stressed
T14794 10284-10286 PRP denotes It
T14793 10283-10557 sentence denotes It should also be stressed that Capn4-/- ES and MEF cells from the presumptive hypomorphic allele can be maintained in culture despite an apparent lack of calpain activity, as assessed by casein zymography or by the appearance of characteristic spectrin breakdown products.
T14792 10282-10283 . denotes .
T14791 10261-10270 JJ denotes embryonic
T14790 10271-10282 NN denotes development
T14789 10255-10260 JJ denotes early
T14788 10252-10254 IN denotes on
T14787 10244-10251 NNS denotes effects
T14786 10237-10243 JJ denotes lethal
T14785 10227-10231 RB denotes both
T14784 10221-10226 MD denotes would
T14783 10198-10208 NN denotes chromosome
T14782 10209-10220 NN denotes segregation
T14781 10188-10197 JJ denotes defective
T14780 10185-10187 CC denotes or
T14779 10232-10236 VB denotes have
T14778 10174-10184 NN denotes Polyspermy
T14777 10173-10283 sentence denotes Polyspermy or defective chromosome segregation would both have lethal effects on early embryonic development.
T14776 10172-10173 . denotes .
T14775 10171-10172 -RRB- denotes ]
T14774 10169-10171 CD denotes 45
T14773 10168-10169 -LRB- denotes [
T14772 10156-10167 NN denotes segregation
T14771 10145-10155 NN denotes chromosome
T14770 10142-10144 IN denotes in
T14769 10130-10132 VB denotes be
T14768 10124-10129 MD denotes could
T14767 10121-10123 PRP denotes it
T14766 10133-10141 VBN denotes involved
T14765 10116-10120 VBD denotes were
T14764 10114-10116 , denotes ,
T14763 10101-10114 NN denotes fertilization
T14762 10095-10100 IN denotes after
T14761 10079-10086 JJ denotes meiotic
T14760 10072-10078 NN denotes oocyte
T14759 10087-10094 NN denotes spindle
T14758 10068-10071 DT denotes the
T14757 10065-10067 IN denotes to
T14756 10048-10052 RB denotes also
T14755 10053-10064 VBD denotes relocalized
T14754 10039-10040 HYPH denotes -
T14753 10040-10047 NN denotes calpain
T14752 10038-10039 NN denotes m
T14751 10037-10173 sentence denotes m-calpain also relocalized to the oocyte meiotic spindle after fertilization, were it could be involved in chromosome segregation [45].
T14750 10036-10037 . denotes .
T14749 10035-10036 -RRB- denotes ]
T14748 10033-10035 CD denotes 45
T14747 10032-10033 -LRB- denotes [
T14746 10021-10031 NN denotes polyspermy
T14745 10013-10020 VB denotes prevent
T14744 10010-10012 TO denotes to
T14743 10001-10009 VBN denotes required
T14742 9984-9991 NN denotes granule
T14741 9992-10000 NNS denotes contents
T14740 9975-9983 JJ denotes cortical
T14739 9972-9974 IN denotes of
T14738 9964-9971 NN denotes release
T14737 9960-9963 DT denotes the
T14736 9957-9959 IN denotes in
T14735 9939-9944 MD denotes might
T14734 9936-9938 PRP denotes it
T14733 9945-9956 VB denotes participate
T14732 9930-9935 WRB denotes where
T14731 9922-9929 NNS denotes oocytes
T14730 9919-9921 IN denotes in
T14729 9901-9909 JJ denotes cortical
T14728 9910-9918 NN denotes membrane
T14727 9897-9900 DT denotes the
T14726 9894-9896 IN denotes to
T14725 9885-9886 HYPH denotes -
T14724 9886-9893 NN denotes calpain
T14723 9884-9885 NN denotes m
T14722 9881-9883 IN denotes of
T14721 9867-9880 NN denotes translocation
T14720 9865-9866 DT denotes a
T14719 9860-9864 IN denotes with
T14718 9849-9859 VBD denotes correlated
T14717 9843-9848 WDT denotes which
T14716 9841-9842 -RRB- denotes ]
T14715 9839-9841 CD denotes 47
T14714 9838-9839 -LRB- denotes [
T14713 9820-9828 NN denotes acrosome
T14712 9829-9837 NN denotes reaction
T14711 9816-9819 DT denotes the
T14710 9813-9815 IN denotes in
T14709 9798-9801 VBD denotes was
T14708 9789-9790 HYPH denotes -
T14707 9790-9797 NN denotes calpain
T14706 9788-9789 NN denotes m
T14705 9786-9787 . denotes .
T15161 12267-12268 DT denotes a
T14704 9785-9786 -RRB- denotes ]
T14703 9783-9785 CD denotes 45
T14702 9782-9783 -LRB- denotes [
T14701 9774-9781 NNS denotes oocytes
T14700 9770-9773 CC denotes and
T14699 9768-9769 -RRB- denotes ]
T14698 9766-9768 CD denotes 47
T14697 9765-9766 -LRB- denotes [
T14696 9759-9764 NN denotes sperm
T14695 9752-9758 NN denotes rodent
T14694 9749-9751 IN denotes in
T14693 9802-9812 VBN denotes implicated
T14692 9735-9739 VBN denotes been
T14691 9726-9734 RB denotes recently
T14690 9721-9725 VBP denotes have
T14689 9740-9748 VBN denotes detected
T14688 9712-9713 HYPH denotes -
T14687 9711-9712 NN denotes m
T14686 9707-9710 CC denotes and
T14685 9705-9706 HYPH denotes -
T14684 9713-9720 NN denotes calpain
T14683 9704-9705 NN denotes μ
T14682 9699-9703 CC denotes Both
T14681 9698-10037 sentence denotes Both μ- and m-calpain have recently been detected in rodent sperm [47] and oocytes [45]. m-calpain was implicated in the acrosome reaction [47] which correlated with a translocation of m-calpain to the cortical membrane in oocytes where it might participate in the release of cortical granule contents required to prevent polyspermy [45].
T14680 9697-9698 . denotes .
T14679 9696-9697 -RRB- denotes ]
T14678 9694-9696 CD denotes 46
T14677 9693-9694 -LRB- denotes [
T14676 9675-9683 NN denotes acrosome
T14675 9684-9692 NN denotes reaction
T14674 9671-9674 DT denotes the
T14673 9662-9670 VBZ denotes triggers
T14672 9656-9661 WDT denotes which
T14671 9636-9647 JJ denotes cytoplasmic
T14670 9648-9655 NN denotes calcium
T14609 9346-9350 VBN denotes been
T14608 9341-9345 RB denotes also
T14607 9337-9340 VBZ denotes has
T14606 9351-9361 VBN denotes associated
T14605 9329-9336 NN denotes Calpain
T14604 9328-9422 sentence denotes Calpain has also been associated with progression through the G1 stage of the cell cycle [6].
T14603 9327-9328 . denotes .
T14602 9326-9327 -RRB- denotes ]
T14601 9325-9326 CD denotes 5
T14600 9324-9325 -LRB- denotes [
T14599 9309-9313 NN denotes cell
T14598 9314-9323 NNS denotes densities
T14597 9305-9308 JJ denotes low
T14596 9300-9304 RB denotes very
T14595 9292-9296 RB denotes only
T14594 9297-9299 IN denotes at
T14593 9288-9291 CC denotes but
T14592 9286-9288 , denotes ,
T14591 9273-9286 NN denotes proliferation
T14590 9268-9272 NN denotes cell
T14589 9258-9267 VB denotes influence
T14588 9255-9257 TO denotes to
T14587 9245-9248 VBD denotes was
T14586 9237-9244 NN denotes calpain
T14585 9235-9237 , denotes ,
T14584 9229-9230 SYM denotes -
T14583 9228-9229 HYPH denotes /
T14582 9227-9228 SYM denotes -
T14581 9231-9235 NNS denotes MEFs
T14580 9222-9227 NN denotes Capn4
T14579 9218-9221 CC denotes and
T14578 9208-9211 NN denotes CHO
T14577 9212-9217 NNS denotes cells
T14576 9203-9207 CC denotes both
T14575 9249-9254 VBN denotes shown
T14574 9200-9202 IN denotes In
T14573 9199-9328 sentence denotes In both CHO cells and Capn4-/- MEFs, calpain was shown to influence cell proliferation, but only at very low cell densities [5].
T14572 9198-9199 . denotes .
T14571 9197-9198 -RRB- denotes ]
T14570 9195-9196 , denotes ,
T14569 9194-9195 CD denotes 5
T14568 9196-9197 CD denotes 6
T14567 9193-9194 -LRB- denotes [
T14566 9183-9192 JJ denotes essential
T14565 9174-9182 RB denotes strictly
T14564 9162-9169 NN denotes calpain
T14563 9170-9173 VBD denotes was
T14562 9157-9161 IN denotes that
T14561 9148-9151 RB denotes not
T14560 9152-9156 VB denotes show
T14559 9144-9147 VBD denotes did
T14558 9140-9143 CC denotes and
T14557 9138-9140 , denotes ,
T14556 9130-9131 HYPH denotes -
T14555 9129-9130 NN denotes m
T14554 9125-9128 CC denotes and
T14553 9123-9124 HYPH denotes -
T14552 9131-9138 NN denotes calpain
T14551 9122-9123 NN denotes μ
T14550 9114-9121 IN denotes between
T14549 9098-9101 RB denotes not
T14548 9094-9097 VBD denotes did
T14547 9083-9093 RB denotes frequently
T14546 9069-9077 VBN denotes reported
T14545 9078-9082 NN denotes work
T14544 9065-9068 DT denotes the
T14543 9102-9113 VB denotes distinguish
T14542 9056-9064 IN denotes although
T14541 9054-9056 , denotes ,
T14540 9044-9048 NN denotes cell
T14539 9049-9054 NNS denotes lines
T14538 9038-9043 JJ denotes other
T14537 9034-9037 CC denotes and
T14536 9027-9028 SYM denotes -
T14535 9026-9027 HYPH denotes /
T14534 9025-9026 SYM denotes -
T14533 9029-9033 NNS denotes MEFs
T14532 9020-9025 NN denotes Capn4
T14531 9017-9019 IN denotes in
T14530 9006-9010 VBN denotes been
T14529 9002-9005 VBZ denotes has
T14528 8992-9001 NN denotes viability
T14527 8987-8991 NN denotes cell
T14526 8983-8986 CC denotes and
T14525 8975-8982 NN denotes calpain
T14524 8967-8974 IN denotes between
T14523 9011-9016 VBN denotes noted
T14522 8955-8966 NN denotes association
T14521 8951-8954 DT denotes The
T14520 8950-9199 sentence denotes The association between calpain and cell viability has been noted in Capn4-/- MEFs and other cell lines, although the reported work frequently did not distinguish between μ- and m-calpain, and did not show that calpain was strictly essential [5,6].
T14519 8949-8950 . denotes .
T14518 8928-8937 JJ denotes embryonic
T14517 8938-8949 NN denotes development
T14516 8921-8927 VBN denotes failed
T14515 8918-8920 IN denotes to
T14514 8901-8906 MD denotes could
T14513 8899-8901 , denotes ,
T14512 8898-8899 -RRB- denotes ]
T14511 8897-8898 CD denotes 9
T14510 8896-8897 -LRB- denotes [
T14509 8888-8889 SYM denotes -
T14508 8887-8888 HYPH denotes /
T14507 8886-8887 SYM denotes -
T14506 8890-8895 NNS denotes cells
T14505 8881-8886 NN denotes Capn4
T14504 8878-8880 IN denotes in
T14503 8869-8877 VBN denotes reported
T14502 8867-8869 , denotes ,
T14501 8858-8867 NN denotes migration
T14500 8855-8857 IN denotes in
T14499 8907-8917 VB denotes contribute
T14498 8847-8854 NNS denotes Defects
T14497 8846-8950 sentence denotes Defects in migration, reported in Capn4-/- cells [9], could contribute to failed embryonic development.
T14496 8845-8846 . denotes .
T14495 8844-8845 -RRB- denotes ]
T14494 8842-8844 CD denotes 45
T14493 8841-8842 -LRB- denotes [
T14492 8833-8840 NN denotes meiosis
T14491 8826-8832 IN denotes during
T14490 8818-8822 RB denotes well
T14489 8823-8825 IN denotes as
T14488 8815-8817 RB denotes as
T14487 8813-8814 -RRB- denotes ]
T14486 8811-8813 CD denotes 44
T14485 8810-8811 -LRB- denotes [
T14484 8802-8809 NN denotes mitosis
T14483 8795-8801 IN denotes during
T14482 8783-8794 NN denotes segregation
T14481 8772-8782 NN denotes chromosome
T14480 8769-8771 IN denotes in
T14479 8757-8768 NN denotes involvement
T14478 8753-8756 PRP$ denotes its
T14477 8750-8752 IN denotes of
T14476 8742-8749 NNS denotes reports
T14475 8732-8741 VBG denotes including
T14474 8730-8732 , denotes ,
T14473 8717-8730 NNS denotes circumstances
T14472 8709-8716 JJ denotes certain
T14471 8706-8708 IN denotes in
T14470 8692-8705 NN denotes proliferation
T14469 8687-8691 NN denotes cell
T14468 8684-8686 IN denotes in
T14467 8672-8683 NN denotes involvement
T14466 8663-8664 HYPH denotes -
T14465 8664-8671 NN denotes calpain
T14464 8662-8663 NN denotes m
T14463 8659-8661 IN denotes of
T14462 8651-8658 NNS denotes reports
T14461 8643-8650 JJ denotes several
T14460 8633-8637 VBP denotes have
T14459 8638-8642 VBN denotes been
T14458 8627-8632 EX denotes There
T14457 8626-8846 sentence denotes There have been several reports of m-calpain involvement in cell proliferation in certain circumstances, including reports of its involvement in chromosome segregation during mitosis [44] as well as during meiosis [45].
T14456 8625-8626 . denotes .
T14455 8618-8625 JJ denotes unclear
T14454 8612-8617 RB denotes still
T14453 8599-8600 HYPH denotes -
T14452 8600-8607 NN denotes calpain
T14451 8598-8599 NN denotes m
T14450 8595-8597 IN denotes of
T14449 8587-8594 NN denotes absence
T14448 8583-8586 DT denotes the
T14447 8580-8582 IN denotes in
T14446 8570-8579 NN denotes lethality
T14445 8566-8569 IN denotes for
T14444 8608-8611 VBP denotes are
T14443 8546-8557 JJ denotes mechanistic
T14442 8558-8565 NNS denotes reasons
T14441 8542-8545 DT denotes The
T14440 8541-8626 sentence denotes The mechanistic reasons for lethality in the absence of m-calpain are still unclear.
T14439 8540-8541 . denotes .
T14438 8526-8530 NN denotes cell
T14437 8531-8540 NNS denotes divisions
T14436 8523-8524 SYM denotes –
T14435 8524-8525 CD denotes 3
T14434 8522-8523 CD denotes 2
T14433 8514-8521 IN denotes through
T14432 8506-8513 NN denotes protein
T14431 8503-8505 CC denotes or
T14430 8502-8503 HYPH denotes /
T14429 8499-8502 CC denotes and
T14944 11093-11103 JJ denotes homozygous
T14943 11111-11116 NN denotes state
T14942 11089-11092 DT denotes the
T14941 11125-11136 VBZ denotes compromises
T14940 11084-11088 IN denotes that
T14939 11070-11074 NN denotes G418
T14938 11067-11069 IN denotes of
T14937 11052-11066 NNS denotes concentrations
T14936 11047-11051 JJ denotes high
T14935 11044-11046 IN denotes in
T14934 11037-11043 NNS denotes clones
T14933 11034-11036 IN denotes of
T14932 11024-11033 NN denotes selection
T14931 11021-11023 IN denotes by
T14930 11015-11020 NNS denotes cells
T14929 11012-11014 NN denotes ES
T14928 11010-11011 SYM denotes -
T14927 11009-11010 HYPH denotes /
T14926 11008-11009 SYM denotes -
T14925 11003-11008 NN denotes Capn2
T14924 11000-11002 IN denotes to
T14923 10998-10999 SYM denotes -
T14922 10997-10998 HYPH denotes /
T14921 10996-10997 SYM denotes +
T14920 10991-10996 NN denotes Capn2
T14919 10988-10990 IN denotes of
T14918 10977-10987 NN denotes conversion
T14917 10972-10976 NN denotes gene
T14916 10964-10971 VB denotes achieve
T14915 10961-10963 TO denotes to
T14914 11075-11083 VBZ denotes suggests
T14913 10944-10952 VBN denotes repeated
T14912 10953-10960 NN denotes failure
T14911 10940-10943 DT denotes The
T14910 10939-11184 sentence denotes The repeated failure to achieve gene conversion of Capn2+/- to Capn2-/- ES cells by selection of clones in high concentrations of G418 suggests that the homozygous mutant state somehow compromises cell viability or clonal selection of ES cells.
T14909 10938-10939 . denotes .
T14908 10923-10932 JJ denotes embryonic
T14907 10933-10938 NNS denotes cells
T14906 10917-10922 JJ denotes early
T14905 10914-10916 IN denotes in
T14904 10907-10913 JJ denotes absent
T14903 10903-10906 VBP denotes are
T14902 10898-10902 WDT denotes that
T14901 10887-10891 NN denotes cell
T14900 10892-10897 NNS denotes lines
T14899 10881-10886 DT denotes these
T14898 10878-10880 IN denotes in
T14897 10866-10871 MD denotes might
T14896 10856-10865 NN denotes viability
T14895 10851-10855 NN denotes cell
T14894 10840-10850 VBG denotes protecting
T14893 10836-10839 IN denotes for
T14892 10825-10835 NNS denotes mechanisms
T14891 10812-10813 HYPH denotes -
T14890 10813-10824 JJ denotes independent
T14889 10805-10812 NN denotes calpain
T14888 10803-10805 , denotes ,
T14887 10872-10877 VB denotes exist
T14886 10792-10803 RB denotes Furthermore
T14428 8494-8498 NN denotes mRNA
T14427 8485-8486 HYPH denotes -
T14426 8484-8485 NN denotes m
T14425 8475-8483 JJ denotes maternal
T14424 8486-8493 NN denotes calpain
T14423 8470-8474 DT denotes some
T14422 8467-8469 IN denotes of
T14421 8455-8466 NN denotes persistence
T14420 8451-8454 DT denotes the
T14419 8448-8450 IN denotes to
T14418 8444-8447 IN denotes due
T14417 8437-8443 RB denotes likely
T14416 8432-8436 RBS denotes most
T14415 8417-8418 HYPH denotes -
T14414 8418-8422 NN denotes cell
T14413 8416-8417 CD denotes 8
T14412 8423-8428 NN denotes stage
T14411 8412-8415 DT denotes the
T14410 8409-8411 IN denotes to
T14409 8399-8400 SYM denotes -
T14408 8398-8399 HYPH denotes /
T14407 8397-8398 SYM denotes -
T14406 8392-8397 NN denotes Capn2
T14405 8388-8391 JJ denotes few
T14404 8401-8408 NNS denotes embryos
T14403 8386-8387 DT denotes a
T14402 8383-8385 IN denotes of
T14401 8374-8382 NN denotes survival
T14400 8370-8373 DT denotes the
T14399 8368-8370 , denotes ,
T14398 8359-8368 NN denotes viability
T14397 8354-8358 NN denotes cell
T14396 8351-8353 IN denotes of
T14395 8344-8350 NN denotes aspect
T14394 8339-8343 DT denotes some
T14376 8267-8271 IN denotes into
T14375 8262-8266 VBZ denotes fits
T14374 8247-8252 NN denotes Capn2
T14373 8253-8261 NN denotes knockout
T14372 8243-8246 DT denotes The
T14371 8242-8292 sentence denotes The Capn2 knockout fits into the latter category.
T14370 8241-8242 . denotes .
T14369 8230-8232 VB denotes be
T14368 8226-8229 RB denotes not
T14367 8220-8225 MD denotes could
T14366 8233-8241 VBN denotes isolated
T14365 8211-8213 NN denotes ES
T14364 8204-8210 NN denotes mutant
T14363 8214-8219 NNS denotes cells
T14362 8193-8203 JJ denotes homozygous
T14361 8189-8192 CC denotes and
T14360 8175-8179 RB denotes ever
T14359 8171-8174 VBP denotes are
T14358 8158-8162 JJ denotes null
T14357 8154-8157 JJ denotes few
T14356 8152-8153 DT denotes a
T14355 8163-8170 NNS denotes embryos
T14354 8147-8151 RB denotes only
T14353 8145-8147 , denotes ,
T14352 8136-8145 NNS denotes instances
T14351 8130-8135 DT denotes these
T14350 8180-8188 VBN denotes observed
T14349 8127-8129 IN denotes In
T14348 8126-8242 sentence denotes In these instances, only a few null embryos are ever observed and homozygous mutant ES cells could not be isolated.
T14347 8125-8126 . denotes .
T14346 8124-8125 -RRB- denotes ]
T14345 8122-8124 CD denotes 43
T14344 8121-8122 SYM denotes -
T14343 8118-8119 , denotes ,
T14342 8117-8118 CD denotes 3
T14341 8119-8121 CD denotes 39
T14340 8116-8117 -LRB- denotes [
T14339 8103-8108 JJ denotes early
T14338 8109-8115 NN denotes embryo
T14337 8099-8102 DT denotes the
T14336 8096-8098 IN denotes in
T14335 8090-8095 NNS denotes cells
T14334 8087-8089 IN denotes of
T14333 8077-8086 NN denotes viability
T14332 8073-8076 DT denotes the
T14331 8065-8072 RB denotes fatally
T14330 8054-8064 VB denotes compromise
T14329 8051-8053 TO denotes to
T14328 8040-8042 VBZ denotes is
T14327 8024-8028 NN denotes gene
T14326 8029-8039 NN denotes disruption
T14325 8020-8023 DT denotes the
T14324 8018-8020 , denotes ,
T14323 8011-8018 RB denotes however
T14322 8009-8011 , denotes ,
T14321 8004-8009 NNS denotes cases
T14320 7998-8003 JJ denotes other
T14319 8043-8050 VBN denotes thought
T14318 7995-7997 IN denotes In
T14317 7994-8126 sentence denotes In other cases, however, the gene disruption is thought to compromise fatally the viability of cells in the early embryo [3,39-43].
T14316 7993-7994 . denotes .
T14315 7980-7987 RB denotes usually
T14314 7977-7979 VBZ denotes is
T14313 7967-7976 NNS denotes genotypes
T14312 7957-7966 JJ denotes embryonic
T14311 7954-7956 IN denotes of
T14310 7988-7993 VBN denotes noted
T14309 7931-7940 JJ denotes Mendelian
T14308 7941-7953 NN denotes distribution
T14307 7929-7930 DT denotes a
T14306 7925-7928 CC denotes and
T14305 7923-7925 , denotes ,
T14304 7911-7917 NN denotes morula
T14303 7918-7923 NN denotes stage
T14302 7907-7910 DT denotes the
T14301 7900-7906 IN denotes beyond
T14300 7886-7891 RB denotes often
T14299 7881-7885 NN denotes type
T14298 7876-7880 DT denotes this
T14297 7873-7875 IN denotes of
T14296 7892-7899 VBP denotes survive
T14295 7865-7872 NNS denotes embryos
T14294 7860-7864 JJ denotes Null
T14293 7859-7994 sentence denotes Null embryos of this type often survive beyond the morula stage, and a Mendelian distribution of embryonic genotypes is usually noted.
T14292 7858-7859 . denotes .
T14291 7857-7858 -RRB- denotes ]
T14290 7855-7857 CD denotes 38
T14289 7854-7855 -LRB- denotes [
T14288 7838-7853 RB denotes morphologically
T14287 7826-7828 VB denotes be
T14286 7820-7825 RB denotes often
T14285 7816-7819 MD denotes can
T14284 7829-7837 VBN denotes observed
T14283 7810-7815 WDT denotes which
T14282 7786-7801 NN denotes differentiation
T14281 7776-7785 JJ denotes embryonic
T14280 7802-7809 NN denotes program
T14279 7772-7775 DT denotes the
T14278 7769-7771 IN denotes in
T14277 7756-7759 VBP denotes are
T14276 7748-7755 NNS denotes defects
T14275 7746-7748 , denotes ,
T14274 7741-7746 NNS denotes cases
T14273 7736-7740 DT denotes some
T14272 7760-7768 VBN denotes incurred
T14271 7733-7735 IN denotes In
T14270 7732-7859 sentence denotes In some cases, defects are incurred in the embryonic differentiation program which can often be observed morphologically [38].
T14269 7731-7732 . denotes .
T14268 7717-7724 JJ denotes general
T14267 7725-7731 NNS denotes causes
T14266 7713-7716 CD denotes two
T14265 7710-7712 IN denotes to
T14264 7696-7698 VB denotes be
T14263 7686-7695 RB denotes typically
T14262 7682-7685 MD denotes can
T14261 7672-7681 NNS denotes knockouts
T14260 7667-7671 NN denotes gene
T14259 7662-7666 IN denotes from
T14258 7652-7661 VBG denotes resulting
T14257 7699-7709 VBN denotes attributed
T14256 7642-7651 NN denotes lethality
T14255 7626-7641 NN denotes Preimplantation
T14254 7625-7732 sentence denotes Preimplantation lethality resulting from gene knockouts can typically be attributed to two general causes.
T14253 7624-7625 . denotes .
T14252 7615-7624 VBG denotes revealing
T14251 7608-7614 PRP denotes itself
T14250 7605-7607 IN denotes in
T14249 7600-7601 SYM denotes -
T14248 7599-7600 HYPH denotes /
T14247 7598-7599 SYM denotes -
T14246 7593-7598 NN denotes Capn2
T14245 7590-7592 VB denotes be
T14244 7587-7589 TO denotes to
T14243 7555-7571 JJ denotes pre-implantation
T14242 7545-7554 VBN denotes genotyped
T14241 7532-7544 RB denotes successfully
T14240 7526-7528 IN denotes of
T14239 7522-7525 IN denotes out
T14238 7529-7531 CD denotes 90
T14237 7518-7521 CD denotes two
T14236 7572-7579 NNS denotes embryos
T14235 7513-7517 RB denotes only
T14234 7580-7586 VBD denotes proved
T14233 7508-7512 IN denotes that
T14232 7503-7507 NN denotes fact
T14231 7499-7502 DT denotes the
T14230 7497-7499 , denotes ,
T14229 7602-7604 VBZ denotes is
T14228 7490-7497 RB denotes However
T14227 7489-7625 sentence denotes However, the fact that only two out of 90 successfully genotyped pre-implantation embryos proved to be Capn2-/- is in itself revealing.
T14226 7488-7489 . denotes .
T14225 7467-7480 JJ denotes morphological
T14224 7459-7466 JJ denotes obvious
T14223 7481-7488 NNS denotes defects
T14222 7455-7458 DT denotes any
T14221 7443-7446 RB denotes not
T14220 7439-7442 VBD denotes did
T14219 7427-7428 HYPH denotes -
T14218 7428-7432 NN denotes cell
T14217 7426-7427 CD denotes 8
T14216 7433-7438 NN denotes stage
T14215 7422-7425 DT denotes the
T14214 7419-7421 IN denotes at
T14213 7408-7418 VBN denotes identified
T14212 7447-7454 VB denotes present
T14211 7395-7399 JJ denotes null
T14210 7384-7394 JJ denotes homozygous
T14209 7380-7383 CD denotes two
T14208 7400-7407 NNS denotes embryos
T14207 7376-7379 DT denotes The
T14206 7375-7489 sentence denotes The two homozygous null embryos identified at the 8-cell stage did not present any obvious morphological defects.
T14205 7374-7375 . denotes .
T14204 7360-7364 VBN denotes been
T14203 7356-7359 RB denotes yet
T14202 7352-7355 RB denotes not
T14201 7348-7351 VBZ denotes has
T14200 7338-7339 SYM denotes -
T14199 7337-7338 HYPH denotes /
T14198 7336-7337 SYM denotes -
T14197 7340-7347 NNS denotes embryos
T14196 7331-7336 NN denotes Capn2
T14669 9626-9635 VBN denotes increased
T14668 9623-9625 IN denotes to
T14667 9610-9616 NN denotes oocyte
T14666 9606-9609 DT denotes the
T14665 9603-9605 IN denotes to
T14664 9617-9622 VBZ denotes leads
T14663 9595-9602 NN denotes binding
T14662 9589-9594 NN denotes Sperm
T14661 9588-9698 sentence denotes Sperm binding to the oocyte leads to increased cytoplasmic calcium which triggers the acrosome reaction [46].
T14660 9587-9588 . denotes .
T14659 9586-9587 -RRB- denotes ]
T14658 9585-9586 CD denotes 3
T14657 9584-9585 -LRB- denotes [
T14656 9573-9583 NNS denotes substrates
T14655 9565-9572 NN denotes calpain
T14654 9562-9564 VB denotes be
T14653 9559-9561 TO denotes to
T14652 9547-9550 VBP denotes are
T14651 9539-9546 NN denotes p27kip1
T14650 9535-9538 CC denotes and
T14649 9533-9535 , denotes ,
T14648 9531-9533 NN denotes D1
T14647 9524-9530 NN denotes cyclin
T14646 9522-9524 , denotes ,
T14645 9518-9522 NN denotes p107
T14644 9516-9518 , denotes ,
T14643 9513-9516 NN denotes p53
T14642 9510-9512 IN denotes as
T14641 9505-9509 JJ denotes such
T14640 9503-9505 , denotes ,
T14639 9492-9503 NN denotes progression
T14638 9486-9491 NN denotes cycle
T14637 9481-9485 NN denotes cell
T14636 9478-9480 IN denotes in
T14635 9466-9468 VB denotes be
T14634 9469-9477 VBN denotes involved
T14633 9463-9465 TO denotes to
T14632 9457-9462 VBN denotes known
T14631 9448-9456 NN denotes proteins
T14630 9444-9447 DT denotes the
T14629 9441-9443 IN denotes of
T14628 9436-9440 DT denotes some
T14627 9434-9436 , denotes ,
T14626 9551-9558 VBN denotes reputed
T14625 9423-9434 RB denotes Furthermore
T14624 9422-9588 sentence denotes Furthermore, some of the proteins known to be involved in cell cycle progression, such as p53, p107, cyclin D1, and p27kip1 are reputed to be calpain substrates [3].
T14623 9421-9422 . denotes .
T14622 9420-9421 -RRB- denotes ]
T14621 9419-9420 CD denotes 6
T14620 9418-9419 -LRB- denotes [
T14619 9407-9411 NN denotes cell
T14618 9412-9417 NN denotes cycle
T14617 9403-9406 DT denotes the
T14616 9400-9402 IN denotes of
T14615 9391-9393 NN denotes G1
T14614 9394-9399 NN denotes stage
T14613 9387-9390 DT denotes the
T14612 9379-9386 IN denotes through
T14611 9367-9378 NN denotes progression
T14610 9362-9366 IN denotes with
T14195 7328-7330 IN denotes in
T14194 7302-7317 NN denotes preimplantation
T14193 7318-7327 NN denotes lethality
T14192 7298-7301 DT denotes the
T14191 7295-7297 IN denotes of
T14190 7365-7374 VBN denotes clarified
T14189 7278-7288 VBG denotes underlying
T14188 7289-7294 NN denotes cause
T14187 7274-7277 DT denotes The
T14186 7273-7375 sentence denotes The underlying cause of the preimplantation lethality in Capn2-/- embryos has not yet been clarified.
T14185 7272-7273 . denotes .
T14184 7264-7272 JJ denotes distinct
T14183 7251-7252 HYPH denotes -
T14182 7252-7259 NN denotes calpain
T14181 7250-7251 NN denotes m
T14180 7246-7249 CC denotes and
T14179 7237-7238 HYPH denotes -
T14178 7238-7245 NN denotes calpain
T14177 7236-7237 NN denotes μ
T14176 7233-7235 IN denotes of
T14175 7218-7222 DT denotes some
T14174 7223-7232 NNS denotes functions
T14173 7212-7217 RBS denotes least
T14172 7209-7211 RB denotes at
T14171 7260-7263 VBP denotes are
T14170 7204-7208 IN denotes that
T14169 7196-7203 VBZ denotes follows
T14168 7193-7195 PRP denotes It
T14167 7192-7273 sentence denotes It follows that at least some functions of μ-calpain and m-calpain are distinct.
T14166 7191-7192 . denotes .
T14165 7180-7181 HYPH denotes -
T14164 7181-7185 NN denotes cell
T14163 7179-7180 CD denotes 8
T14162 7186-7191 NN denotes stage
T14161 7175-7178 DT denotes the
T14040 6559-6566 NNS denotes amounts
T14039 6544-6549 PRP$ denotes their
T14038 6541-6543 IN denotes of
T14037 6531-6540 NNS denotes estimates
T14036 6522-6530 JJ denotes reliable
T14035 6510-6513 RB denotes not
T14034 6507-6509 VBP denotes do
T14033 6505-6507 , denotes ,
T14032 6484-6493 JJ denotes available
T14031 6494-6505 NN denotes methodology
T14030 6480-6483 DT denotes the
T14029 6477-6479 IN denotes in
T14028 6466-6476 NNS denotes weaknesses
T14027 6463-6465 IN denotes to
T14026 6457-6462 VBG denotes owing
T14025 6455-6457 , denotes ,
T14024 6441-6450 VBN denotes published
T14023 6451-6455 NNS denotes data
T14022 6437-6440 DT denotes the
T14021 6514-6521 VB denotes provide
T14020 6428-6436 IN denotes although
T14019 6426-6428 , denotes ,
T14018 6411-6420 JJ denotes mammalian
T14017 6421-6426 NNS denotes cells
T14016 6406-6410 JJS denotes most
T14015 6403-6405 IN denotes in
T14014 6395-6402 JJ denotes present
T14013 6391-6394 VBP denotes are
T14012 6371-6372 HYPH denotes -
T14011 6370-6371 NN denotes m
T14010 6366-6369 CC denotes and
T14009 6364-6365 HYPH denotes -
T14008 6372-6379 NN denotes calpain
T14007 6363-6364 NN denotes μ
T14006 6380-6390 NNS denotes activities
T14005 6358-6362 CC denotes Both
T14004 6357-6567 sentence denotes Both μ- and m-calpain activities are present in most mammalian cells, although the published data, owing to weaknesses in the available methodology, do not provide reliable estimates of their relative amounts.
T14003 6356-6357 . denotes .
T14002 6346-6356 NN denotes activation
T14001 6337-6345 NN denotes platelet
T14000 6334-6336 IN denotes in
T13999 6325-6333 VBN denotes involved
T13998 6316-6324 NN denotes proteins
T13997 6308-6315 JJ denotes certain
T13996 6305-6307 IN denotes of
T13995 6280-6288 NN denotes tyrosine
T13994 6289-6304 NN denotes phosphorylation
T13993 6276-6279 DT denotes the
T13992 6273-6275 IN denotes of
T13991 6267-6272 NN denotes level
T13990 6263-6266 DT denotes the
T13989 6260-6262 IN denotes at
T13988 6251-6259 RB denotes possibly
T13987 6249-6251 , denotes ,
T13986 6241-6249 NN denotes function
T13985 6232-6240 NN denotes platelet
T13984 6229-6231 IN denotes in
T13983 6217-6228 NN denotes disturbance
T13982 6215-6216 DT denotes a
T13981 6195-6205 NN denotes deficiency
T13980 6189-6194 NN denotes Capn1
T13979 6186-6188 IN denotes of
T13978 6179-6185 NN denotes result
T13977 6177-6178 DT denotes a
T13976 6174-6176 IN denotes as
T13975 6165-6173 VBN denotes observed
T13974 6206-6214 VBD denotes involved
T13973 6145-6154 JJ denotes principal
T13972 6155-6164 NN denotes phenotype
T13971 6141-6144 DT denotes The
T13970 6140-6357 sentence denotes The principal phenotype observed as a result of Capn1 deficiency involved a disturbance in platelet function, possibly at the level of the tyrosine phosphorylation of certain proteins involved in platelet activation.
T13969 6139-6140 . denotes .
T13968 6138-6139 -RRB- denotes ]
T13967 6136-6138 CD denotes 33
T13966 6135-6136 -LRB- denotes [
T13965 6130-6134 NNS denotes mice
T13964 6127-6129 IN denotes of
T13963 6117-6126 NN denotes viability
T13962 6113-6116 DT denotes the
T13961 6102-6105 RB denotes not
T13960 6098-6101 VBD denotes did
T13959 6096-6098 , denotes ,
T13958 6091-6096 NN denotes Capn1
T13957 6089-6091 , denotes ,
T13956 6077-6084 NN denotes subunit
T13955 6071-6076 JJ denotes large
T13954 6062-6063 HYPH denotes -
T13953 6063-6070 NN denotes calpain
T13952 6061-6062 NN denotes μ
T13951 6085-6089 NN denotes gene
T13950 6057-6060 DT denotes the
T13949 6054-6056 IN denotes of
T13948 6043-6053 NN denotes disruption
T13947 6032-6042 JJ denotes homozygous
T13946 6030-6032 , denotes ,
T13945 6018-6030 NN denotes implantation
T13944 6015-6017 IN denotes to
T13943 6009-6014 JJ denotes prior
T13942 5990-5996 JJ denotes murine
T13941 5997-6004 NNS denotes embryos
T13940 5985-5989 JJ denotes null
T13939 5979-5984 NN denotes Capn2
T13938 6005-6008 VBP denotes die
T13937 5971-5978 IN denotes whereas
T13936 5969-5971 , denotes ,
T13935 6106-6112 VB denotes affect
T13934 5964-5969 VBN denotes noted
T13933 5961-5963 IN denotes As
T13932 5960-6140 sentence denotes As noted, whereas Capn2 null murine embryos die prior to implantation, homozygous disruption of the μ-calpain large subunit gene, Capn1, did not affect the viability of mice [33].
T13931 5959-5960 . denotes .
T13930 5946-5959 NN denotes embryogenesis
T13929 5939-5945 IN denotes during
T13928 5933-5938 RBS denotes least
T13927 5930-5932 RB denotes at
T13926 5928-5930 , denotes ,
T13925 5909-5922 JJ denotes physiological
T13924 5900-5908 JJ denotes distinct
T13923 5923-5928 NNS denotes roles
T13922 5895-5899 DT denotes some
T13921 5881-5882 HYPH denotes -
T13920 5880-5881 NN denotes m
T13919 5876-5879 CC denotes and
T13918 5874-5875 HYPH denotes -
T13917 5882-5889 NN denotes calpain
T13916 5873-5874 NN denotes μ
T13915 5890-5894 VBP denotes have
T13914 5868-5872 IN denotes that
T13913 5866-5868 , denotes ,
T13912 5862-5866 NNS denotes mice
T13911 5859-5861 IN denotes in
T13910 5854-5858 VBN denotes done
T13909 5839-5848 NN denotes targeting
T13908 5834-5838 NN denotes gene
T13907 5849-5853 NN denotes work
T13906 5830-5833 DT denotes the
T13905 5825-5829 IN denotes from
T13904 5823-5825 , denotes ,
T13903 5818-5823 JJ denotes clear
T13902 5814-5817 RB denotes now
T13901 5811-5813 VBZ denotes is
T13900 5808-5810 PRP denotes It
T13899 5807-5960 sentence denotes It is now clear, from the gene targeting work done in mice, that μ- and m-calpain have some distinct physiological roles, at least during embryogenesis.
T13898 5806-5807 . denotes .
T13897 5801-5806 NNS denotes cells
T13896 5794-5800 IN denotes inside
T13895 5769-5783 RB denotes differentially
T13894 5765-5768 VBP denotes are
T13893 5752-5755 CD denotes two
T13892 5756-5764 NNS denotes isoforms
T13891 5748-5751 DT denotes the
T13890 5784-5793 VBN denotes regulated
T13889 5743-5747 IN denotes that
T13888 5734-5742 JJ denotes possible
T13887 5728-5730 PRP denotes it
T13886 5726-5728 , denotes ,
T13885 5716-5726 NN denotes activation
T13884 5710-5715 FW denotes vitro
T13883 5707-5709 FW denotes in
T13882 5703-5706 IN denotes for
T13881 5701-5702 SYM denotes +
T13880 5698-5701 NN denotes Ca2
T13879 5695-5697 IN denotes of
T13878 5687-5694 NNS denotes amounts
T13877 5677-5686 JJ denotes different
T13876 5664-5668 PRP denotes they
T13875 5669-5676 VBP denotes require
T13874 5658-5663 IN denotes since
T13873 5656-5658 , denotes ,
T13872 5646-5651 JJ denotes other
T13871 5652-5656 NN denotes hand
T13870 5642-5645 DT denotes the
T13869 5731-5733 VBZ denotes is
T13868 5639-5641 IN denotes On
T13867 5638-5807 sentence denotes On the other hand, since they require different amounts of Ca2+ for in vitro activation, it is possible that the two isoforms are differentially regulated inside cells.
T13866 5637-5638 . denotes .
T13865 5633-5637 FW denotes vivo
T13864 5630-5632 FW denotes in
T13863 5615-5619 JJ denotes same
T13862 5620-5629 NNS denotes functions
T13861 5611-5614 DT denotes the
T13860 5607-5610 RP denotes out
T14393 8335-8338 IN denotes for
T14392 8325-8334 JJ denotes essential
T14391 8313-8314 HYPH denotes -
T14390 8314-8321 NN denotes calpain
T14389 8312-8313 NN denotes m
T14388 8322-8324 VBZ denotes is
T14387 8307-8311 IN denotes that
T14386 8302-8306 JJ denotes true
T14385 8429-8431 VBZ denotes is
T14384 8296-8298 PRP denotes it
T14383 8299-8301 VBZ denotes is
T14382 8293-8295 IN denotes If
T14381 8292-8541 sentence denotes If it is true that m-calpain is essential for some aspect of cell viability, the survival of a few Capn2-/- embryos to the 8-cell stage is most likely due to the persistence of some maternal m-calpain mRNA and/or protein through 2–3 cell divisions.
T14380 8291-8292 . denotes .
T14379 8276-8282 JJ denotes latter
T14378 8283-8291 NN denotes category
T14377 8272-8275 DT denotes the
T13859 5601-5606 VB denotes carry
T13858 5598-5600 TO denotes to
T13857 5588-5597 NN denotes potential
T13856 5584-5587 DT denotes the
T13855 5574-5578 PRP denotes they
T13854 5579-5583 VBP denotes have
T13853 5569-5573 IN denotes that
T13852 5558-5568 VBG denotes suggesting
T13851 5556-5558 , denotes ,
T13850 5551-5556 FW denotes vitro
T13849 5548-5550 FW denotes in
T13848 5537-5547 NNS denotes substrates
T13847 5534-5536 IN denotes of
T13846 5525-5529 JJ denotes same
T13845 5530-5533 NN denotes set
T13844 5521-5524 DT denotes the
T13843 5509-5520 RB denotes essentially
T13842 5497-5501 PRP denotes they
T13841 5495-5497 , denotes ,
T13840 5502-5508 VBP denotes cleave
T13839 5488-5495 RB denotes Notably
T13838 5487-5638 sentence denotes Notably, they cleave essentially the same set of substrates in vitro, suggesting that they have the potential to carry out the same functions in vivo.
T13837 5486-5487 . denotes .
T13836 5474-5486 NN denotes biochemistry
T13835 5470-5473 CC denotes and
T13834 5460-5469 NN denotes structure
T13833 5454-5459 PRP$ denotes their
T13832 5451-5453 IN denotes in
T13831 5443-5450 JJ denotes similar
T13830 5438-5442 RB denotes very
T13829 5434-5437 VBP denotes are
T13828 5430-5433 CC denotes and
T13827 5412-5420 NN denotes sequence
T13826 5421-5429 NN denotes identity
T13825 5410-5411 NN denotes %
T13745 5002-5008 NN denotes timing
T13744 4988-4991 DT denotes The
T13743 4987-5239 sentence denotes The different timing of lethality in the Capn4 knockouts might also be a consequence of the different genetic backgrounds of the two transgenic lines, which has been observed to influence the phenotype of transgenic mice on a number of occasions [37].
T13742 4986-4987 . denotes .
T13741 4985-4986 -RRB- denotes ]
T13740 4983-4985 CD denotes 36
T13739 4982-4983 -LRB- denotes [
T13738 4976-4981 NNS denotes cells
T13737 4966-4975 JJ denotes mammalian
T13736 4963-4965 IN denotes in
T13735 4960-4962 CC denotes or
T13734 4955-4959 NNP denotes coli
T13733 4952-4954 NNP denotes E.
T13732 4949-4951 IN denotes in
T13731 4942-4948 CC denotes either
T13730 4936-4941 RB denotes alone
T13729 4921-4925 VBD denotes were
T13728 4906-4911 JJ denotes large
T13727 4912-4920 NNS denotes subunits
T13726 4898-4905 NN denotes calpain
T13725 4926-4935 VBN denotes expressed
T13724 4893-4897 WRB denotes when
T13723 4887-4892 NNS denotes hands
T13722 4883-4886 PRP$ denotes our
T13721 4880-4882 IN denotes in
T13720 4867-4870 VBD denotes was
T13719 4871-4879 VBN denotes observed
T13718 4850-4857 NN denotes calpain
T13717 4858-4866 NN denotes activity
T13716 4847-4849 DT denotes no
T13715 4843-4846 CC denotes and
T13714 4841-4843 , denotes ,
T13713 4830-4841 NN denotes possibility
T13712 4825-4829 DT denotes that
T13711 4817-4824 VB denotes exclude
T13710 4814-4816 TO denotes to
T13709 4793-4796 FW denotes al.
T13708 4790-4792 FW denotes et
T13707 4780-4789 NNP denotes Zimmerman
T13706 4797-4805 NN denotes knockout
T13705 4776-4779 DT denotes the
T13704 4806-4813 VBZ denotes appears
T13703 4767-4775 IN denotes although
T13702 4765-4767 , denotes ,
T13701 4760-4765 NNS denotes cells
T13700 4749-4759 JJ denotes eukaryotic
T13699 4746-4748 IN denotes in
T13698 4737-4745 NN denotes activity
T13697 4732-4736 DT denotes some
T13696 4718-4723 MD denotes might
T13695 4712-4717 RB denotes alone
T13694 4697-4702 JJ denotes large
T13693 4703-4711 NNS denotes subunits
T13692 4689-4696 NN denotes calpain
T13691 4724-4731 VB denotes provide
T13690 4684-4688 IN denotes that
T13689 4669-4673 VBN denotes been
T13688 4664-4668 RB denotes also
T13687 4660-4663 VBZ denotes has
T13686 4657-4659 PRP denotes It
T13685 4655-4656 -RRB- denotes )
T13684 4651-4655 NN denotes work
T13683 4639-4650 JJ denotes unpublished
T13682 4637-4639 , denotes ,
T13681 4633-4637 NNP denotes Elce
T13680 4628-4632 NNP denotes J.S.
T13679 4627-4628 -LRB- denotes (
T13678 4613-4615 VB denotes be
T13677 4616-4626 VBN denotes determined
T13676 4610-4612 TO denotes to
T13675 4606-4609 RB denotes yet
T13674 4596-4601 NNS denotes cells
T13673 4586-4595 JJ denotes mammalian
T13672 4583-4585 IN denotes in
T13671 4569-4582 NN denotes functionality
T13670 4563-4568 PRP$ denotes their
T13669 4602-4605 VBZ denotes has
T13668 4554-4562 IN denotes although
T13667 4552-4554 , denotes ,
T13666 4548-4552 NNP denotes coli
T13665 4545-4547 NNP denotes E.
T13664 4542-4544 IN denotes in
T13663 4532-4541 VBN denotes expressed
T13662 4527-4531 WRB denotes when
T13661 4510-4517 NN denotes calpain
T13660 4499-4509 JJ denotes detectable
T13659 4518-4526 NN denotes activity
T13658 4495-4498 DT denotes any
T13657 4492-4494 IN denotes to
T13656 4487-4491 NN denotes rise
T13655 4674-4683 VBN denotes suggested
T13654 4478-4481 RB denotes not
T13653 4474-4477 VBD denotes did
T13652 4459-4464 JJ denotes small
T13651 4450-4458 VBN denotes modified
T13650 4465-4473 NNS denotes subunits
T13649 4444-4449 DT denotes these
T13648 4439-4443 IN denotes with
T13647 4430-4438 NNS denotes calpains
T13646 4427-4429 IN denotes of
T13645 4416-4426 NN denotes expression
T13644 4414-4416 , denotes ,
T13643 4482-4486 VB denotes give
T13642 4407-4414 RB denotes However
T13641 4406-4987 sentence denotes However, expression of calpains with these modified small subunits did not give rise to any detectable calpain activity when expressed in E. coli, although their functionality in mammalian cells has yet to be determined (J.S. Elce, unpublished work) It has also been suggested that calpain large subunits alone might provide some activity in eukaryotic cells, although the Zimmerman et al. knockout appears to exclude that possibility, and no calpain activity was observed in our hands when calpain large subunits were expressed alone either in E. coli or in mammalian cells [36].
T13640 4405-4406 . denotes .
T13639 4397-4405 NN denotes activity
T13638 4389-4396 NN denotes calpain
T13637 4386-4388 IN denotes of
T13636 4376-4379 JJ denotes low
T13635 4380-4385 NN denotes level
T13634 4374-4375 DT denotes a
T13633 4366-4373 VB denotes support
T13632 4363-4365 TO denotes to
T13631 4352-4362 JJ denotes sufficient
T13630 4343-4348 MD denotes might
T13629 4349-4351 VB denotes be
T13628 4337-4342 WDT denotes which
T13627 4335-4337 , denotes ,
T13626 4320-4321 HYPH denotes -
T13625 4321-4329 JJ denotes terminal
T13624 4319-4320 NN denotes C
T13623 4305-4318 JJ denotes inappropriate
T13622 4330-4335 NNS denotes acids
T13621 4301-4302 SYM denotes –
T13620 4302-4304 CD denotes 30
T13619 4299-4301 CD denotes 10
T13618 4294-4298 IN denotes with
T13617 4279-4284 JJ denotes small
T13616 4271-4278 NN denotes calpain
T13615 4285-4293 NNS denotes subunits
T13614 4261-4270 JJ denotes defective
T13613 4258-4260 IN denotes to
T13612 4253-4257 NN denotes rise
T13611 4242-4247 MD denotes could
T13610 4248-4252 VB denotes give
T13609 4230-4241 NNS denotes transcripts
T13608 4224-4229 DT denotes These
T13607 4223-4406 sentence denotes These transcripts could give rise to defective calpain small subunits with 10–30 inappropriate C-terminal acids, which might be sufficient to support a low level of calpain activity.
T13606 4222-4223 . denotes .
T13605 4221-4222 -RRB- denotes ]
T13604 4219-4221 CD denotes 31
T13603 4218-4219 -LRB- denotes [
T13602 4200-4208 NN denotes promoter
T13601 4196-4199 NN denotes PGK
T13600 4209-4217 NN denotes sequence
T13599 4192-4195 DT denotes the
T13598 4189-4191 IN denotes in
T13597 4176-4182 NN denotes splice
T13596 4168-4175 JJ denotes cryptic
T13595 4158-4167 JJ denotes different
T13594 4183-4188 NNS denotes sites
T13593 4148-4153 RBS denotes least
T13592 4154-4157 CD denotes two
T13591 4145-4147 RB denotes at
T13590 4142-4144 IN denotes to
T13589 4140-4141 CD denotes 9
T13588 4135-4139 NN denotes exon
T13587 4132-4134 IN denotes of
T13586 4121-4126 JJ denotes first
T13585 4127-4131 NN denotes half
T13584 4117-4120 DT denotes the
T13583 4112-4116 IN denotes from
T13582 4104-4111 RB denotes through
T13581 4096-4103 VBG denotes reading
T13580 4094-4096 , denotes ,
T13579 4090-4091 HYPH denotes -
T13578 4091-4094 NN denotes PCR
T13577 4088-4090 NN denotes RT
T13576 4085-4087 IN denotes by
T13575 4074-4084 JJ denotes detectable
T13574 4072-4074 , denotes ,
T13573 4060-4064 NN denotes mRNA
T13572 4065-4072 NNS denotes species
T13571 4051-4059 JJ denotes multiple
T13570 4048-4050 IN denotes to
T13569 4043-4047 NN denotes rise
T13568 4038-4042 VBD denotes gave
T13567 4031-4037 NN denotes allele
T13566 4026-4030 DT denotes This
T13565 4025-4223 sentence denotes This allele gave rise to multiple mRNA species, detectable by RT-PCR, reading through from the first half of exon 9 to at least two different cryptic splice sites in the PGK promoter sequence [31].
T14160 7168-7174 IN denotes beyond
T14159 7156-7167 NN denotes development
T14158 7146-7155 JJ denotes embryonic
T14157 7142-7145 IN denotes for
T14156 7132-7141 JJ denotes essential
T14155 7120-7128 NN denotes activity
T14154 7111-7112 HYPH denotes -
T14153 7112-7119 NN denotes calpain
T14152 7110-7111 NN denotes m
T14151 7129-7131 VBZ denotes is
T14150 7105-7109 IN denotes that
T14149 7082-7083 SYM denotes -
T14148 7081-7082 HYPH denotes /
T14147 7080-7081 SYM denotes -
T14146 7084-7091 NNS denotes embryos
T14145 7075-7080 NN denotes Capn2
T14144 7072-7074 IN denotes in
T14143 7062-7071 NN denotes lethality
T14142 7060-7062 , denotes ,
T14141 7052-7060 NN denotes contrast
T14140 7092-7104 VBZ denotes demonstrates
T14139 7049-7051 IN denotes In
T14138 7048-7192 sentence denotes In contrast, lethality in Capn2-/- embryos demonstrates that m-calpain activity is essential for embryonic development beyond the 8-cell stage.
T14137 7047-7048 . denotes .
T14136 7038-7047 JJ denotes essential
T14135 7034-7037 RB denotes not
T14134 7027-7033 RB denotes simply
T14133 7014-7015 HYPH denotes -
T14132 7015-7022 NN denotes calpain
T14131 7013-7014 NN denotes μ
T14130 7010-7012 IN denotes of
T14129 7000-7009 NNS denotes functions
T14128 6996-6999 DT denotes the
T14127 7023-7026 VBP denotes are
T14126 6988-6995 IN denotes whether
T14125 6985-6987 CC denotes or
T14124 6983-6985 , denotes ,
T14123 6966-6967 HYPH denotes -
T14122 6967-6974 NN denotes calpain
T14121 6965-6966 NN denotes m
T14120 6955-6964 VBG denotes remaining
T14119 6975-6983 NN denotes activity
T14118 6951-6954 DT denotes the
T14117 6948-6950 IN denotes by
T14116 6928-6940 JJ denotes compensatory
T14115 6941-6947 NN denotes affect
T14114 6926-6927 DT denotes a
T14113 6923-6925 IN denotes to
T14112 6919-6922 IN denotes due
T14111 6906-6907 SYM denotes -
T14110 6905-6906 HYPH denotes /
T14109 6904-6905 SYM denotes -
T14108 6908-6915 NNS denotes animals
T14107 6899-6904 NN denotes Capn1
T14106 6896-6898 IN denotes in
T14105 6886-6895 NN denotes phenotype
T14104 6879-6885 JJ denotes marked
T14103 6876-6878 IN denotes of
T14102 6868-6875 NN denotes absence
T14101 6864-6867 DT denotes the
T14100 6916-6918 VBZ denotes is
T14099 6856-6863 IN denotes whether
T14098 6846-6855 VB denotes determine
T14097 6843-6845 TO denotes to
T14096 6834-6842 JJ denotes possible
T14095 6830-6833 RB denotes not
T14094 6824-6826 PRP denotes it
T14093 6822-6824 , denotes ,
T14092 6816-6822 NN denotes result
T14091 6814-6815 DT denotes a
T14090 6827-6829 VBZ denotes is
T14089 6811-6813 IN denotes As
T14088 6810-7048 sentence denotes As a result, it is not possible to determine whether the absence of marked phenotype in Capn1-/- animals is due to a compensatory affect by the remaining m-calpain activity, or whether the functions of μ-calpain are simply not essential.
T14087 6809-6810 . denotes .
T14086 6799-6803 NN denotes cell
T14085 6804-6809 NNS denotes types
T14084 6793-6798 JJ denotes other
T14083 6790-6792 IN denotes in
T14082 6785-6789 IN denotes than
T14081 6772-6784 NNS denotes erythrocytes
T14080 6768-6771 CC denotes and
T14079 6758-6767 NNS denotes platelets
T14078 6755-6757 IN denotes in
T14077 6748-6754 JJ denotes likely
T14076 6743-6747 RBR denotes less
T14075 6733-6742 RB denotes therefore
T14074 6721-6722 HYPH denotes -
T14073 6722-6729 NN denotes calpain
T14072 6720-6721 NN denotes m
T14071 6717-6719 IN denotes by
T14070 6706-6716 NN denotes deficiency
T14069 6697-6698 HYPH denotes -
T14068 6698-6705 NN denotes calpain
T14067 6696-6697 NN denotes μ
T14066 6692-6695 IN denotes for
T14065 6730-6732 VBZ denotes is
T14064 6679-6691 NN denotes Compensation
T14063 6678-6810 sentence denotes Compensation for μ-calpain deficiency by m-calpain is therefore less likely in platelets and erythrocytes than in other cell types.
T14062 6677-6678 . denotes .
T14061 6667-6677 JJ denotes detectable
T14060 6660-6666 RB denotes barely
T14059 6648-6656 NN denotes activity
T14058 6639-6640 HYPH denotes -
T14057 6640-6647 NN denotes calpain
T14056 6638-6639 NN denotes m
T14055 6657-6659 VBZ denotes is
T14054 6632-6637 IN denotes while
T14053 6630-6632 , denotes ,
T14052 6613-6614 HYPH denotes -
T14051 6614-6621 NN denotes calpain
T14050 6612-6613 NN denotes μ
T14049 6622-6630 NN denotes activity
T14048 6603-6611 JJ denotes abundant
T14047 6582-6594 NNS denotes erythrocytes
T14046 6578-6581 CC denotes and
T14045 6595-6602 VBP denotes contain
T14044 6568-6577 NNS denotes Platelets
T14043 6567-6678 sentence denotes Platelets and erythrocytes contain abundant μ-calpain activity, while m-calpain activity is barely detectable.
T14042 6566-6567 . denotes .
T14041 6550-6558 JJ denotes relative
T13564 4024-4025 . denotes .
T13563 4012-4017 NN denotes Capn4
T13562 4005-4011 NNP denotes Arthur
T13561 4018-4024 NN denotes allele
T13560 4001-4004 DT denotes the
T13559 3996-4000 IN denotes from
T13558 3988-3995 VBN denotes derived
T13557 3977-3987 NNS denotes activities
T13556 3969-3976 NN denotes calpain
T13555 3966-3968 CC denotes or
T13554 3954-3965 NNS denotes transcripts
T13553 3947-3953 VB denotes detect
T13552 3944-3946 TO denotes to
T13551 3934-3938 VBD denotes were
T13550 3939-3943 VBN denotes made
T13549 3926-3933 NNS denotes Efforts
T13548 3925-4025 sentence denotes Efforts were made to detect transcripts or calpain activities derived from the Arthur Capn4 allele.
T13547 3924-3925 . denotes .
T13546 3908-3918 JJ denotes transgenic
T13545 3902-3907 NN denotes Capn4
T13544 3898-3901 CD denotes two
T13543 3919-3924 NNS denotes lines
T13542 3894-3897 DT denotes the
T13541 3891-3893 IN denotes in
T13540 3886-3890 NN denotes work
T13539 3883-3885 IN denotes at
T13538 3874-3879 MD denotes might
T13537 3880-3882 VB denotes be
T13536 3859-3866 JJ denotes similar
T13535 3867-3873 NN denotes effect
T13534 3857-3858 DT denotes A
T13533 3856-3925 sentence denotes A similar effect might be at work in the two Capn4 transgenic lines.
T13532 3855-3856 . denotes .
T13531 3841-3847 NN denotes mutant
T13530 3848-3855 NNS denotes alleles
T13523 3797-3799 IN denotes of
T13522 3788-3796 NN denotes function
T13521 3785-3787 IN denotes of
T13520 3778-3784 NN denotes degree
T13519 3775-3777 IN denotes in
T13518 3763-3774 NNS denotes differences
T13517 3759-3762 DT denotes the
T13516 3756-3758 IN denotes to
T13515 3741-3744 VBD denotes was
T13514 3731-3740 NN denotes phenotype
T13513 3728-3730 IN denotes in
T13512 3745-3755 VBN denotes attributed
T13511 3718-3727 NN denotes variation
T13510 3714-3717 DT denotes The
T13509 3713-3856 sentence denotes The variation in phenotype was attributed to the differences in degree of function of the truncated proteins produced from the mutant alleles.
T13508 3712-3713 . denotes .
T13507 3711-3712 -RRB- denotes ]
T13506 3709-3711 CD denotes 35
T13505 3708-3709 -LRB- denotes [
T13504 3706-3707 -RRB- denotes )
T13503 3699-3701 , denotes ,
T13502 3694-3699 NN denotes E10.5
T13501 3692-3694 , denotes ,
T13500 3688-3692 NN denotes E0.5
T13499 3701-3706 NN denotes E14.5
T13498 3687-3688 -LRB- denotes (
T13497 3680-3686 NNS denotes stages
T13496 3670-3679 JJ denotes different
T13495 3667-3669 IN denotes at
T13494 3663-3666 CC denotes but
T13493 3661-3663 , denotes ,
T13492 3648-3661 NN denotes embryogenesis
T13491 3641-3647 IN denotes during
T13490 3624-3631 NNS denotes embryos
T13489 3619-3623 JJ denotes null
T13488 3608-3618 JJ denotes homozygous
T13487 3606-3608 , denotes ,
T13486 3593-3598 CD denotes three
T13485 3599-3606 NNS denotes studies
T13484 3589-3592 DT denotes all
T13483 3632-3640 VBN denotes perished
T13482 3586-3588 IN denotes In
T13481 3585-3713 sentence denotes In all three studies, homozygous null embryos perished during embryogenesis, but at different stages (E0.5, E10.5, E14.5) [35].
T13480 3584-3585 . denotes .
T13479 3578-3579 -RRB- denotes )
T13478 3575-3578 NN denotes Mll
T13477 3574-3575 -LRB- denotes (
T13476 3557-3564 NN denotes lineage
T13475 3565-3573 NN denotes leukemia
T13474 3551-3556 JJ denotes mixed
T13473 3580-3584 NN denotes gene
T13472 3547-3550 DT denotes the
T13471 3539-3546 VB denotes disrupt
T13470 3536-3538 TO denotes to
T13469 3517-3530 RB denotes independently
T13468 3512-3516 VBD denotes were
T13467 3491-3500 NN denotes targeting
T13466 3481-3490 JJ denotes different
T13465 3501-3511 NNS denotes strategies
T13464 3475-3480 CD denotes three
T13463 3473-3475 , denotes ,
T13462 3466-3473 NN denotes example
T13461 3531-3535 VBN denotes used
T13460 3462-3465 IN denotes For
T13459 3461-3585 sentence denotes For example, three different targeting strategies were independently used to disrupt the mixed lineage leukemia (Mll) gene.
T13458 3460-3461 . denotes .
T13457 3453-3460 NNS denotes studies
T13456 3442-3452 NN denotes disruption
T13455 3437-3441 NN denotes gene
T13454 3434-3436 IN denotes in
T13453 3423-3433 NNS denotes phenotypes
T13452 3413-3422 JJ denotes different
T13451 3407-3412 VB denotes yield
T13450 3404-3406 TO denotes to
T13449 3393-3397 VBN denotes been
T13448 3388-3392 VBP denotes have
T13447 3398-3403 VBN denotes shown
T13446 3367-3376 NN denotes targeting
T13445 3377-3387 NNS denotes strategies
T13444 3357-3366 JJ denotes Alternate
T13443 3356-3461 sentence denotes Alternate targeting strategies have been shown to yield different phenotypes in gene disruption studies.
T13442 3355-3356 . denotes .
T13441 3335-3346 JJ denotes hypomorphic
T13440 3347-3355 NN denotes mutation
T13439 3333-3334 DT denotes a
T13438 3326-3332 RB denotes likely
T13437 3309-3315 NNP denotes Arthur
T13436 3316-3322 NN denotes allele
T13435 3305-3308 DT denotes the
T13434 3323-3325 VBZ denotes is
T13433 3299-3304 IN denotes while
T13432 3285-3289 JJ denotes null
T13431 3280-3284 JJ denotes true
T13430 3290-3298 NN denotes genotype
T13429 3278-3279 DT denotes a
T13428 3258-3266 RB denotes probably
T13427 3267-3277 VBZ denotes represents
T13426 3241-3250 NNP denotes Zimmerman
T13425 3251-3257 NN denotes allele
T13424 3237-3240 DT denotes The
T13423 3236-3356 sentence denotes The Zimmerman allele probably represents a true null genotype while the Arthur allele is likely a hypomorphic mutation.
T13422 3235-3236 . denotes .
T13421 3225-3235 NN denotes disruption
T13420 3222-3224 IN denotes of
T13419 3214-3221 NNS denotes extents
T13418 3204-3213 JJ denotes different
T13417 3201-3203 IN denotes by
T13416 3188-3190 VB denotes be
T13415 3184-3187 MD denotes can
T13414 3172-3177 NN denotes Capn4
T13413 3163-3171 VBN denotes targeted
T13412 3159-3162 CD denotes two
T13411 3178-3183 NNS denotes lines
T13410 3153-3158 DT denotes these
T13409 3150-3152 IN denotes of
T13408 3140-3149 NN denotes lethality
T13407 3137-3139 IN denotes of
T13406 3132-3136 NN denotes time
T13405 3128-3131 DT denotes the
T13404 3125-3127 IN denotes in
T13403 3114-3124 NN denotes difference
T13402 3110-3113 DT denotes the
T13401 3191-3200 VBN denotes explained
T13400 3105-3109 IN denotes that
T13399 3096-3104 JJ denotes probable
T13398 3086-3089 RB denotes now
T13397 3090-3095 VBZ denotes seems
T13396 3083-3085 PRP denotes It
T13395 3082-3236 sentence denotes It now seems probable that the difference in the time of lethality of these two targeted Capn4 lines can be explained by different extents of disruption.
T13394 3081-3082 . denotes .
T13393 3080-3081 -RRB- denotes ]
T13392 3078-3080 CD denotes 32
T13391 3077-3078 -LRB- denotes [
T13390 3075-3076 CD denotes 8
T13389 3067-3074 IN denotes through
T13388 3065-3066 CD denotes 4
T13387 3059-3064 NNS denotes exons
T13386 3056-3058 IN denotes of
T13385 3037-3046 JJ denotes extensive
T13384 3032-3036 RBR denotes more
T13383 3027-3031 RB denotes much
T13382 3047-3055 NN denotes deletion
T13381 3025-3026 DT denotes a
T13380 3005-3015 NNS denotes colleagues
T13379 3001-3004 CC denotes and
T13378 2991-3000 NNP denotes Zimmerman
T13377 2988-2990 IN denotes by
T13376 2979-2987 VBN denotes employed
T13375 2960-2969 VBG denotes targeting
T13374 2954-2959 NN denotes Capn4
T13373 2970-2978 NN denotes strategy
T13372 2950-2953 DT denotes the
T13371 2948-2950 , denotes ,
T13370 2940-2948 NN denotes contrast
T13369 3016-3024 VBD denotes involved
T13368 2937-2939 IN denotes In
T13367 2936-3082 sentence denotes In contrast, the Capn4 targeting strategy employed by Zimmerman and colleagues involved a much more extensive deletion of exons 4 through 8 [32].
T13366 2935-2936 . denotes .
T13365 2934-2935 -RRB- denotes ]
T13364 2932-2934 CD denotes 34
T13363 2931-2932 -LRB- denotes [
T13362 2926-2930 NNP denotes coli
T13361 2923-2925 NNP denotes E.
T13360 2920-2922 IN denotes in
T13359 2906-2911 JJ denotes large
T13358 2897-2898 HYPH denotes -
T13357 2898-2905 NN denotes calpain
T13356 2896-2897 NN denotes m
T13355 2912-2919 NN denotes subunit
T13354 2892-2895 DT denotes the
T13353 2887-2891 IN denotes with
T13352 2874-2886 VBN denotes co-expressed
T13351 2869-2873 WRB denotes when
T13350 2860-2868 NN denotes activity
T13349 2852-2859 NN denotes calpain
T13348 2828-2833 JJ denotes small
T13347 2834-2841 NN denotes subunit
T13346 2824-2827 DT denotes the
T13345 2821-2823 IN denotes of
T13344 2812-2820 VBZ denotes residues
T13343 2801-2806 NN denotes amino
T13824 5408-5410 CD denotes 62
T13823 5402-5407 VBP denotes share
T13822 5390-5393 CD denotes two
T13821 5394-5401 NNS denotes enzymes
T13820 5386-5389 DT denotes The
T13819 5385-5487 sentence denotes The two enzymes share 62% sequence identity and are very similar in their structure and biochemistry.
T13818 5384-5385 . denotes .
T13817 5374-5378 FW denotes vivo
T13816 5371-5373 FW denotes in
T13815 5379-5384 NNS denotes roles
T13814 5362-5370 JJ denotes distinct
T13813 5352-5354 , denotes ,
T13812 5344-5345 HYPH denotes -
T13811 5343-5344 NN denotes m
T13810 5339-5342 CC denotes and
T13809 5337-5338 HYPH denotes -
T13808 5345-5352 NN denotes calpain
T13807 5336-5337 NN denotes μ
T13806 5334-5336 , denotes ,
T13805 5315-5325 JJ denotes ubiquitous
T13804 5311-5314 CD denotes two
T13803 5326-5334 NNS denotes isoforms
T13802 5307-5310 DT denotes the
T13801 5354-5361 VBP denotes possess
T13800 5299-5306 IN denotes whether
T13799 5290-5293 VBZ denotes has
T13798 5281-5289 NN denotes research
T13797 5273-5280 NN denotes calpain
T13796 5270-5272 IN denotes in
T13795 5251-5259 VBG denotes enduring
T13794 5260-5269 NNS denotes questions
T13793 5247-5250 DT denotes the
T13792 5244-5246 IN denotes of
T13791 5294-5298 VBN denotes been
T13790 5240-5243 CD denotes One
T13789 5239-5385 sentence denotes One of the enduring questions in calpain research has been whether the two ubiquitous isoforms, μ- and m-calpain, possess distinct in vivo roles.
T13788 5238-5239 . denotes .
T13787 5237-5238 -RRB- denotes ]
T13786 5235-5237 CD denotes 37
T13785 5234-5235 -LRB- denotes [
T13784 5224-5233 NNS denotes occasions
T13783 5221-5223 IN denotes of
T13782 5214-5220 NN denotes number
T13781 5212-5213 DT denotes a
T13780 5209-5211 IN denotes on
T13779 5204-5208 NNS denotes mice
T13778 5193-5203 JJ denotes transgenic
T13777 5190-5192 IN denotes of
T13776 5180-5189 NN denotes phenotype
T13775 5176-5179 DT denotes the
T13774 5166-5175 VB denotes influence
T13773 5163-5165 TO denotes to
T13772 5149-5153 VBN denotes been
T13771 5145-5148 VBZ denotes has
T13770 5154-5162 VBN denotes observed
T13769 5139-5144 WDT denotes which
T13768 5137-5139 , denotes ,
T13767 5121-5131 JJ denotes transgenic
T13766 5117-5120 CD denotes two
T13765 5132-5137 NNS denotes lines
T13764 5113-5116 DT denotes the
T13763 5110-5112 IN denotes of
T13762 5090-5097 JJ denotes genetic
T13761 5080-5089 JJ denotes different
T13760 5098-5109 NNS denotes backgrounds
T13759 5076-5079 DT denotes the
T13758 5073-5075 IN denotes of
T13757 5061-5072 NN denotes consequence
T13756 5059-5060 DT denotes a
T13755 5051-5055 RB denotes also
T13754 5045-5050 MD denotes might
T13753 5029-5034 NN denotes Capn4
T13752 5035-5044 NNS denotes knockouts
T13751 5025-5028 DT denotes the
T13750 5022-5024 IN denotes in
T13749 5012-5021 NN denotes lethality
T13748 5009-5011 IN denotes of
T13747 5056-5058 VB denotes be
T13746 4992-5001 JJ denotes different
T13342 2798-2800 CD denotes 25
T13341 2788-2789 HYPH denotes -
T13340 2789-2797 JJ denotes terminal
T13339 2787-2788 NN denotes C
T13338 2807-2811 NN denotes acid
T13337 2783-2786 DT denotes the
T13336 2780-2782 IN denotes of
T13335 2771-2779 NN denotes excision
T13334 2842-2851 VBD denotes abolished
T13333 2766-2770 IN denotes that
T13332 2758-2765 VBG denotes showing
T13331 2740-2741 HYPH denotes /
T13330 2741-2749 NN denotes function
T13329 2731-2740 NN denotes structure
T13328 2750-2757 NNS denotes studies
T13327 2722-2730 JJ denotes previous
T13326 2717-2721 IN denotes upon
T13325 2707-2710 VBD denotes was
T13324 2711-2716 VBN denotes based
T13323 2698-2706 NN denotes strategy
T13322 2693-2697 DT denotes This
T13321 2692-2936 sentence denotes This strategy was based upon previous structure/function studies showing that excision of the C-terminal 25 amino acid residues of the small subunit abolished calpain activity when co-expressed with the m-calpain large subunit in E. coli [34].
T13320 2691-2692 . denotes .
T13319 2690-2691 -RRB- denotes ]
T13318 2688-2690 CD denotes 31
T13317 2687-2688 -LRB- denotes [
T13316 2679-2686 NN denotes protein
T13315 2675-2678 DT denotes the
T13314 2672-2674 IN denotes of
T13313 2661-2671 NN denotes truncation
T13312 2654-2660 VBD denotes caused
T13311 2648-2653 WDT denotes which
T13310 2646-2648 , denotes ,
T13309 2645-2646 CD denotes 9
T13308 2640-2644 NN denotes exon
T13282 2495-2497 IN denotes by
T13281 2486-2494 VBN denotes employed
T13280 2520-2528 VBN denotes involved
T13279 2467-2476 NN denotes targeting
T13278 2461-2466 NN denotes Capn4
T13277 2477-2485 NN denotes strategy
T13276 2457-2460 DT denotes The
T13275 2456-2692 sentence denotes The Capn4 targeting strategy employed by Arthur and colleagues involved disrupting the C-terminus of the calpain small subunit by insertion of the PGK-Neo cassette into the middle of exon 9, which caused truncation of the protein [31].
T13274 2455-2456 . denotes .
T13273 2446-2450 JJ denotes true
T13272 2451-2455 NN denotes null
T13271 2444-2445 DT denotes a
T13270 2439-2443 IN denotes than
T13269 2432-2438 RB denotes rather
T13268 2430-2432 , denotes ,
T13267 2413-2424 JJ denotes hypomorphic
T13266 2425-2430 NN denotes state
T13265 2411-2412 DT denotes a
T13264 2388-2394 JJ denotes latter
T13263 2395-2399 NN denotes line
T13262 2384-2387 DT denotes the
T13261 2400-2410 VBZ denotes represents
T13260 2379-2383 IN denotes that
T13259 2372-2378 JJ denotes likely
T13258 2363-2365 PRP denotes it
T13257 2361-2363 , denotes ,
T13256 2351-2361 NN denotes retrospect
T13255 2366-2371 VBZ denotes seems
T13254 2348-2350 IN denotes In
T13253 2347-2456 sentence denotes In retrospect, it seems likely that the latter line represents a hypomorphic state, rather than a true null.
T13252 2346-2347 . denotes .
T13251 2345-2346 -RRB- denotes ]
T13250 2343-2345 CD denotes 31
T13249 2342-2343 -LRB- denotes [
T13248 2332-2341 VB denotes reconcile
T13247 2329-2331 TO denotes to
T13246 2319-2328 JJ denotes difficult
T13245 2314-2318 RBR denotes more
T13244 2297-2310 NN denotes mid-gestation
T13243 2294-2296 IN denotes to
T13242 2283-2293 NNS denotes colleagues
T13241 2279-2282 CC denotes and
T13240 2272-2278 NNP denotes Arthur
T13239 2269-2271 IN denotes by
T13238 2260-2268 VBN denotes reported
T13237 2246-2254 VBN denotes targeted
T13236 2237-2245 JJ denotes original
T13235 2255-2259 NN denotes line
T13234 2233-2236 DT denotes the
T13233 2228-2232 IN denotes from
T13232 2218-2219 SYM denotes -
T13231 2217-2218 HYPH denotes /
T13230 2216-2217 SYM denotes -
T13229 2220-2227 NNS denotes embryos
T13228 2211-2216 NN denotes Capn4
T13227 2208-2210 IN denotes of
T13226 2311-2313 VBZ denotes is
T13225 2199-2207 NN denotes survival
T13224 2195-2198 DT denotes The
T13223 2194-2347 sentence denotes The survival of Capn4-/- embryos from the original targeted line reported by Arthur and colleagues to mid-gestation is more difficult to reconcile [31].
T13222 2193-2194 . denotes .
T13221 2192-2193 -RRB- denotes ]
T13220 2190-2192 CD denotes 32
T13219 2189-2190 -LRB- denotes [
T13218 2175-2179 RB denotes also
T13217 2171-2174 VBD denotes was
T13216 2163-2170 NNS denotes embryos
T13215 2158-2162 JJ denotes null
T13214 2155-2157 IN denotes of
T13213 2145-2154 NN denotes lethality
T13212 2129-2144 NN denotes preimplantation
T13211 2123-2128 WDT denotes which
T13210 2180-2188 VBN denotes observed
T13209 2120-2122 IN denotes in
T13208 2105-2113 VBN denotes targeted
T13207 2099-2104 NN denotes Capn4
T13206 2095-2098 CD denotes two
T13205 2114-2119 NNS denotes lines
T13204 2091-2094 DT denotes the
T13203 2088-2090 IN denotes of
T13202 2084-2087 CD denotes one
T13201 2081-2083 IN denotes by
T13200 2071-2080 VBN denotes presented
T13199 2061-2070 NN denotes phenotype
T13198 2057-2060 DT denotes the
T13197 2052-2056 IN denotes with
T13196 2042-2051 NN denotes agreement
T13195 2039-2041 IN denotes in
T13194 2029-2035 NN denotes result
T13193 2024-2028 DT denotes this
T13192 2022-2024 , denotes ,
T13191 2014-2022 NN denotes activity
T13190 2005-2006 HYPH denotes -
T13189 2006-2013 NN denotes calpain
T13188 2004-2005 NN denotes m
T13187 1996-2003 VB denotes abolish
T13186 1993-1995 TO denotes to
T13185 2036-2038 VBZ denotes is
T13184 1979-1983 RB denotes also
T13183 1975-1978 VBD denotes was
T13182 1964-1969 NN denotes Capn4
T13181 1970-1974 NN denotes gene
T13180 1960-1963 DT denotes the
T13179 1957-1959 IN denotes of
T13178 1946-1956 NN denotes disruption
T13177 1935-1945 JJ denotes homozygous
T13176 1984-1992 VBN denotes expected
T13175 1929-1934 IN denotes Since
T13174 1928-2194 sentence denotes Since homozygous disruption of the Capn4 gene was also expected to abolish m-calpain activity, this result is in agreement with the phenotype presented by one of the two Capn4 targeted lines in which preimplantation lethality of null embryos was also observed [32].
T13173 1927-1928 . denotes .
T13172 1914-1927 NN denotes embryogenesis
T13171 1907-1913 IN denotes during
T13170 1885-1897 RB denotes specifically
T13169 1882-1884 VBZ denotes is
T13168 1873-1874 HYPH denotes -
T13167 1874-1881 NN denotes calpain
T13166 1872-1873 NN denotes m
T13165 1898-1906 VBN denotes required
T13164 1867-1871 IN denotes that
T13163 1854-1857 RB denotes now
T13162 1851-1853 TO denotes to
T13161 1858-1866 VB denotes conclude
T13160 1848-1850 PRP denotes us
T13159 1841-1847 VBZ denotes allows
T13158 1829-1840 NN denotes observation
T13157 1824-1828 DT denotes This
T13156 1823-1928 sentence denotes This observation allows us to now conclude that m-calpain is specifically required during embryogenesis.
T13155 1822-1823 . denotes .
T13154 1811-1822 NN denotes development
T13153 1808-1810 IN denotes of
T13152 1786-1801 NN denotes preimplantation
T13151 1802-1807 NN denotes stage
T13150 1782-1785 DT denotes the
T13149 1779-1781 IN denotes at
T13148 1773-1775 , denotes ,
T13147 1756-1765 JJ denotes catalytic
T13146 1747-1748 HYPH denotes -
T13145 1748-1755 NN denotes calpain
T13144 1746-1747 NN denotes m
T13143 1766-1773 NN denotes subunit
T13142 1742-1745 DT denotes the
T13141 1737-1741 VBP denotes lack
T13140 1731-1736 WDT denotes which
T13139 1729-1731 , denotes ,
T13138 1723-1724 SYM denotes -
T13137 1722-1723 HYPH denotes /
T13136 1721-1722 SYM denotes -
T13135 1725-1729 NNS denotes mice
T13134 1716-1721 NN denotes Capn2
T13133 1775-1778 VBP denotes die
T13132 1711-1715 IN denotes that
T13131 1706-1710 RB denotes here
T13130 1699-1705 VBP denotes report
T13129 1696-1698 PRP denotes We
T13128 1695-1823 sentence denotes We report here that Capn2-/- mice, which lack the m-calpain catalytic subunit, die at the preimplantation stage of development.
T13127 1694-1695 . denotes .
T13126 1685-1694 NN denotes viability
T13125 1675-1684 JJ denotes embryonic
T13124 1664-1674 VBG denotes sustaining
T13123 1660-1663 IN denotes for
T13122 1649-1659 JJ denotes sufficient
T13121 1639-1644 RB denotes alone
T13120 1631-1638 NN denotes isoform
T13119 1624-1630 DT denotes either
T13118 1645-1648 VBD denotes was
T13117 1619-1623 IN denotes that
T13116 1616-1618 CC denotes or
T13115 1614-1616 , denotes ,
T13114 1601-1614 NN denotes embryogenesis
T13113 1594-1600 IN denotes during
T13112 1572-1584 RB denotes specifically
T13111 1568-1571 VBD denotes was
T13110 1559-1560 HYPH denotes -
T13109 1558-1559 NN denotes m
T13108 1560-1567 NN denotes calpain
T13107 1551-1557 DT denotes either
T13106 1585-1593 VBN denotes required
T13105 1546-1550 IN denotes that
T13104 1528-1531 CD denotes two
T13103 1523-1527 JJ denotes last
T13102 1532-1545 NNS denotes possibilities
T13529 3837-3840 DT denotes the
T13528 3832-3836 IN denotes from
T13527 3823-3831 VBN denotes produced
T13526 3804-3813 VBN denotes truncated
T13525 3814-3822 NN denotes proteins
T13524 3800-3803 DT denotes the
T13101 1519-1522 DT denotes the
T13100 1514-1518 IN denotes with
T13099 1504-1508 VBD denotes were
T13098 1501-1503 PRP denotes we
T13097 1499-1501 , denotes ,
T13096 1494-1499 NN denotes point
T13095 1489-1493 DT denotes that
T13094 1509-1513 VBN denotes left
T13093 1486-1488 IN denotes At
T13092 1485-1695 sentence denotes At that point, we were left with the last two possibilities that either m-calpain was specifically required during embryogenesis, or that either isoform alone was sufficient for sustaining embryonic viability.
T13091 1484-1485 . denotes .
T13090 1483-1484 -RRB- denotes ]
T13089 1481-1483 CD denotes 33
T13088 1480-1481 -LRB- denotes [
T13087 1466-1470 VBD denotes were
T13086 1447-1457 NN denotes retraction
T13085 1442-1446 NN denotes clot
T13084 1438-1441 CC denotes and
T13083 1458-1465 NNS denotes defects
T13082 1426-1437 NN denotes aggregation
T13081 1417-1425 NN denotes platelet
T13080 1471-1479 VBN denotes observed
T13079 1408-1416 IN denotes although
T13078 1406-1408 , denotes ,
T13077 1399-1406 JJ denotes fertile
T13076 1395-1398 CC denotes and
T13075 1387-1394 JJ denotes healthy
T13074 1380-1382 , denotes ,
T13073 1363-1372 JJ denotes catalytic
T13072 1354-1355 HYPH denotes -
T13071 1355-1362 NN denotes calpain
T13070 1353-1354 NN denotes μ
T13069 1373-1380 NN denotes subunit
T13068 1349-1352 DT denotes the
T13067 1344-1348 VBP denotes lack
T12957 816-824 NN denotes knockout
T12956 825-829 NNS denotes mice
T12955 810-815 NN denotes Capn4
T12954 807-809 IN denotes in
T12953 797-806 NN denotes lethality
T12952 787-796 JJ denotes embryonic
T12951 778-786 VBD denotes observed
T12950 753-764 JJ denotes independent
T12949 765-777 NNS denotes laboratories
T12948 749-752 CD denotes Two
T12947 748-881 sentence denotes Two independent laboratories observed embryonic lethality in Capn4 knockout mice, albeit at different stages of development [31,32].
T12946 747-748 . denotes .
T12945 739-740 HYPH denotes -
T12944 738-739 NN denotes m
T12943 734-737 CC denotes and
T12942 732-733 HYPH denotes -
T12941 731-732 NN denotes μ
T12940 740-747 NN denotes calpain
T12939 726-730 CC denotes both
T12938 723-725 IN denotes to
T12937 716-722 JJ denotes common
T12936 697-707 JJ denotes regulatory
T12935 691-696 JJ denotes small
T12934 708-715 NN denotes subunit
T12933 687-690 DT denotes the
T12932 678-686 VBG denotes encoding
T12931 676-678 , denotes ,
T12930 666-671 NN denotes Capn4
T12929 672-676 NN denotes gene
T12928 662-665 DT denotes the
T12927 654-661 VB denotes disrupt
T12926 651-653 TO denotes to
T12925 640-645 RB denotes first
T12924 636-639 VBD denotes was
T12923 646-650 VBN denotes used
T12922 631-635 DT denotes This
T12921 630-748 sentence denotes This was first used to disrupt the Capn4 gene, encoding the small regulatory subunit common to both μ- and m-calpain.
T12920 629-630 . denotes .
T12919 613-620 NN denotes calpain
T12918 621-629 NNS denotes isoforms
T12917 602-612 JJ denotes individual
T12916 599-601 IN denotes of
T12915 581-592 JJ denotes physiologic
T12914 593-598 NNS denotes roles
T12913 577-580 DT denotes the
T12912 569-576 VB denotes examine
T12911 566-568 TO denotes to
T12910 548-556 JJ denotes powerful
T12909 557-565 NN denotes approach
T12908 546-547 DT denotes a
T12907 533-536 VBZ denotes has
T12906 528-532 NNS denotes mice
T12905 525-527 IN denotes in
T12904 537-545 VBN denotes provided
T12903 515-524 NN denotes targeting
T12902 510-514 NN denotes Gene
T12901 509-630 sentence denotes Gene targeting in mice has provided a powerful approach to examine the physiologic roles of individual calpain isoforms.
T12900 508-509 . denotes .
T12899 487-496 NN denotes substrate
T12898 497-508 NN denotes specificity
T12897 477-486 JJ denotes different
T12896 472-476 VBP denotes have
T12895 468-471 CC denotes and
T12894 446-457 RB denotes differently
T12893 443-445 VB denotes be
T12892 439-442 MD denotes may
T12891 458-467 VBN denotes regulated
T12890 433-438 WDT denotes which
T12889 424-432 NNS denotes isoforms
T12888 416-423 JJ denotes unknown
T12887 405-415 RB denotes previously
T12886 402-404 IN denotes of
T12885 395-401 NN denotes number
T12884 393-394 DT denotes a
T12883 390-392 IN denotes of
T12882 380-389 NN denotes discovery
T12881 376-379 DT denotes the
T12880 373-375 IN denotes by
T12879 353-360 RB denotes further
T12878 348-352 VBN denotes been
T12877 344-347 VBZ denotes has
T12876 338-343 NN denotes story
T12875 334-337 DT denotes the
T12874 332-334 , denotes ,
T12873 321-325 JJ denotes past
T12872 326-332 NN denotes decade
T12871 317-320 DT denotes the
T12870 361-372 VBN denotes complicated
T12869 314-316 IN denotes In
T12868 313-509 sentence denotes In the past decade, the story has been further complicated by the discovery of a number of previously unknown isoforms which may be differently regulated and have different substrate specificity.
T12867 312-313 . denotes .
T12866 303-312 NNS denotes proteases
T12865 297-302 JJ denotes other
T12864 287-296 VBG denotes affecting
T12863 279-286 IN denotes without
T12862 270-278 NNS denotes calpains
T12861 266-269 DT denotes the
T12860 254-255 HYPH denotes -
T12859 250-254 RB denotes down
T12858 255-265 VBG denotes regulating
T12857 237-249 RB denotes specifically
T12856 234-236 IN denotes of
T12855 226-233 JJ denotes capable
T12854 215-225 NNS denotes inhibitors
T12853 212-214 IN denotes of
T12852 207-211 NN denotes lack
T12851 203-206 DT denotes the
T12850 200-202 IN denotes to
T12849 196-199 IN denotes due
T12848 187-190 VBZ denotes has
T12847 182-186 DT denotes this
T12846 180-182 , denotes ,
T12845 176-180 NN denotes part
T12844 170-175 JJ denotes large
T12843 191-195 VBN denotes been
T12842 167-169 IN denotes In
T12841 166-313 sentence denotes In large part, this has been due to the lack of inhibitors capable of specifically down-regulating the calpains without affecting other proteases.
T12840 165-166 . denotes .
T12839 158-165 JJ denotes elusive
T12838 145-148 VBZ denotes has
T12837 137-144 NNS denotes enzymes
T12836 133-136 DT denotes the
T12835 130-132 IN denotes of
T12834 120-129 NNS denotes functions
T12833 116-119 CC denotes and
T12832 105-115 NNS denotes substrates
T12831 101-104 DT denotes the
T12830 98-100 IN denotes of
T12829 78-83 JJ denotes clear
T12828 84-97 NN denotes understanding
T12827 76-77 DT denotes a
T12826 74-76 , denotes ,
T12825 71-74 RB denotes ago
T12824 63-70 NNS denotes decades
T12823 58-62 CD denotes four
T12822 149-157 VBN denotes remained
T12821 41-46 RB denotes first
T12820 37-40 VBD denotes was
T12819 28-36 NN denotes activity
T12818 20-27 NN denotes calpain
T12817 47-57 VBN denotes identified
T12816 11-19 IN denotes Although
T13307 2637-2639 IN denotes of
T13306 2630-2636 NN denotes middle
T13305 2626-2629 DT denotes the
T13304 2621-2625 IN denotes into
T13303 2607-2608 HYPH denotes -
T13302 2608-2611 NN denotes Neo
T13301 2604-2607 NN denotes PGK
T13300 2612-2620 NN denotes cassette
T13299 2600-2603 DT denotes the
T13298 2597-2599 IN denotes of
T13297 2587-2596 NN denotes insertion
T13296 2584-2586 IN denotes by
T13295 2570-2575 JJ denotes small
T13294 2562-2569 NN denotes calpain
T13293 2576-2583 NN denotes subunit
T13292 2558-2561 DT denotes the
T13291 2555-2557 IN denotes of
T13290 2545-2546 HYPH denotes -
T13289 2544-2545 NN denotes C
T13288 2546-2554 NN denotes terminus
T13287 2540-2543 DT denotes the
T13286 2529-2539 VBG denotes disrupting
T13285 2509-2519 NNS denotes colleagues
T13284 2505-2508 CC denotes and
T13283 2498-2504 NNP denotes Arthur
T13066 1338-1343 WDT denotes which
T13065 1336-1338 , denotes ,
T13064 1330-1331 SYM denotes -
T13063 1329-1330 HYPH denotes /
T13062 1328-1329 SYM denotes -
T13061 1332-1336 NNS denotes mice
T13060 1323-1328 NN denotes Capn1
T13059 1382-1386 VBD denotes were
T13058 1318-1322 IN denotes that
T13057 1295-1305 JJ denotes subsequent
T13056 1306-1317 NN denotes observation
T13055 1291-1294 DT denotes the
T13054 1288-1290 IN denotes by
T13053 1283-1287 RP denotes down
T13052 1269-1273 VBD denotes were
T13051 1274-1282 VBN denotes narrowed
T13050 1261-1268 NNS denotes options
T13049 1255-1260 DT denotes These
T13048 1254-1485 sentence denotes These options were narrowed down by the subsequent observation that Capn1-/- mice, which lack the μ-calpain catalytic subunit, were healthy and fertile, although platelet aggregation and clot retraction defects were observed [33].
T13047 1253-1254 . denotes .
T13046 1241-1244 VBD denotes was
T13045 1233-1240 NN denotes isoform
T13044 1227-1232 JJ denotes other
T13043 1223-1226 DT denotes the
T13042 1220-1222 CC denotes or
T13041 1245-1253 VBN denotes required
T13040 1216-1219 CD denotes one
T13039 1212-1215 CC denotes and
T13038 1210-1212 , denotes ,
T13037 1201-1210 JJ denotes redundant
T13036 1188-1189 HYPH denotes -
T13035 1187-1188 NN denotes m
T13034 1183-1186 CC denotes and
T13033 1181-1182 HYPH denotes -
T13032 1189-1196 NN denotes calpain
T13031 1180-1181 NN denotes μ
T13030 1178-1179 -RRB- denotes )
T13029 1197-1200 VBP denotes are
T13028 1177-1178 LS denotes 4
T13027 1174-1176 CC denotes or
T13026 1172-1174 , denotes ,
T13025 1160-1163 VBD denotes was
T13024 1151-1152 HYPH denotes -
T13023 1152-1159 NN denotes calpain
T13022 1150-1151 NN denotes m
T13021 1148-1149 -RRB- denotes )
T13020 1164-1172 VBN denotes required
T13019 1147-1148 LS denotes 3
T13018 1145-1146 : denotes ;
T13017 1133-1136 VBD denotes was
T13016 1124-1125 HYPH denotes -
T13015 1125-1132 NN denotes calpain
T13014 1123-1124 NN denotes μ
T13013 1121-1122 -RRB- denotes )
T13012 1137-1145 VBN denotes required
T13011 1120-1121 LS denotes 2
T13010 1118-1119 : denotes ;
T13009 1105-1109 VBD denotes were
T13008 1096-1104 NNS denotes isoforms
T13007 1091-1095 DT denotes both
T13006 1089-1090 -RRB- denotes )
T13005 1088-1089 LS denotes 1
T13004 1086-1088 : denotes :
T13003 1075-1086 NN denotes development
T13002 1065-1074 JJ denotes embryonic
T13001 1061-1064 IN denotes for
T13000 1049-1060 NN denotes requirement
T12999 1043-1048 PRP$ denotes their
T12998 1033-1042 VBG denotes regarding
T12997 1019-1032 NNS denotes possibilities
T12996 1014-1018 CD denotes four
T12995 1004-1013 VBD denotes suggested
T12994 992-1003 RB denotes furthermore
T12993 988-991 CC denotes and
T12992 986-988 , denotes ,
T12991 978-979 HYPH denotes -
T12990 977-978 NN denotes m
T12989 973-976 CC denotes and
T12988 971-972 HYPH denotes -
T12987 970-971 NN denotes μ
T12986 979-986 NN denotes calpain
T12985 965-969 CC denotes both
T12984 961-964 IN denotes for
T12983 949-951 VBZ denotes is
T12982 935-940 JJ denotes small
T12981 941-948 NN denotes subunit
T12980 931-934 DT denotes the
T12979 952-960 VBN denotes required
T12978 926-930 IN denotes that
T12977 915-925 NN denotes hypothesis
T12976 911-914 DT denotes the
T12975 1110-1118 VBN denotes required
T12974 901-910 VBD denotes supported
T12973 888-900 NNS denotes observations
T12972 882-887 DT denotes These
T12971 881-1254 sentence denotes These observations supported the hypothesis that the small subunit is required for both μ- and m-calpain, and furthermore suggested four possibilities regarding their requirement for embryonic development: 1) both isoforms were required; 2) μ-calpain was required; 3) m-calpain was required, or 4) μ- and m-calpain are redundant, and one or the other isoform was required.
T12970 880-881 . denotes .
T12969 879-880 -RRB- denotes ]
T12968 876-877 , denotes ,
T12967 874-876 CD denotes 31
T12966 877-879 CD denotes 32
T12965 873-874 -LRB- denotes [
T12964 861-872 NN denotes development
T12963 858-860 IN denotes of
T12962 851-857 NNS denotes stages
T12961 841-850 JJ denotes different
T12960 838-840 IN denotes at
T12959 831-837 IN denotes albeit
T12958 829-831 , denotes ,
T15181 12400-12401 . denotes .
T15180 12396-12400 DT denotes this
T15179 12392-12395 IN denotes for
T15178 12374-12385 JJ denotes mechanistic
T15177 12386-12391 NN denotes basis
T15176 12372-12373 DT denotes a
T15175 12360-12364 MD denotes will
T15174 12352-12359 NNS denotes studies
T15173 12345-12351 JJ denotes future
T15172 12365-12371 VB denotes reveal
T15171 12337-12344 RB denotes Perhaps
T15170 12336-12401 sentence denotes Perhaps future studies will reveal a mechanistic basis for this.
T15169 12335-12336 . denotes .
T15160 12259-12266 VBP denotes suggest
T15159 12246-12258 NNS denotes observations
T15158 12240-12245 DT denotes These
T15157 12239-12336 sentence denotes These observations suggest a developmental advantage associated with reduced calpain expression.
T15156 12238-12239 . denotes .
T15155 12237-12238 -RRB- denotes ]
T15154 12235-12237 CD denotes 32
T15153 12234-12235 -LRB- denotes [
T15152 12212-12216 JJ denotes null
T15151 12201-12211 JJ denotes homozygous
T15150 12217-12224 NN denotes progeny
T15149 12198-12200 DT denotes no
T15148 12225-12233 VBN denotes observed
T15147 12193-12197 IN denotes with
T15146 12191-12193 , denotes ,
T15145 12179-12191 JJ denotes heterozygous
T15144 12174-12178 VBD denotes were
T15143 12172-12173 NN denotes %
T15142 12168-12172 CD denotes 77.5
T15141 12164-12167 CC denotes and
T15140 12158-12159 HYPH denotes -
T15139 12159-12163 NN denotes type
T15138 12154-12158 JJ denotes wild
T15168 12317-12324 NN denotes calpain
T15167 12325-12335 NN denotes expression
T15166 12309-12316 VBN denotes reduced
T15165 12304-12308 IN denotes with
T15164 12293-12303 VBN denotes associated
T15163 12269-12282 JJ denotes developmental
T15162 12283-12292 NN denotes advantage
R2370 T12875 T12876 det the,story
R2373 T12816 T12817 mark Although,identified
R2374 T12876 T12870 nsubjpass story,complicated
R2375 T12877 T12870 aux has,complicated
R2376 T12817 T12822 advcl identified,remained
R2377 T12818 T12819 compound calpain,activity
R2378 T12878 T12870 auxpass been,complicated
R2379 T12819 T12817 nsubjpass activity,identified
R2380 T12820 T12817 auxpass was,identified
R2381 T12821 T12817 advmod first,identified
R2382 T12879 T12870 advmod further,complicated
R2383 T12823 T12824 nummod four,decades
R2384 T12880 T12870 agent by,complicated
R2385 T12824 T12825 npadvmod decades,ago
R2386 T12825 T12817 advmod ago,identified
R2387 T12826 T12822 punct ", ",remained
R2388 T12881 T12882 det the,discovery
R2389 T12827 T12828 det a,understanding
R2390 T12828 T12822 nsubj understanding,remained
R2391 T12882 T12880 pobj discovery,by
R2392 T12829 T12828 amod clear,understanding
R2393 T12830 T12828 prep of,understanding
R2394 T12831 T12832 det the,substrates
R2395 T12883 T12882 prep of,discovery
R2396 T12884 T12885 det a,number
R2397 T12832 T12830 pobj substrates,of
R2398 T12885 T12883 pobj number,of
R2399 T12833 T12832 cc and,substrates
R2400 T12834 T12832 conj functions,substrates
R2401 T12886 T12885 prep of,number
R2402 T12835 T12832 prep of,substrates
R2403 T12836 T12837 det the,enzymes
R2404 T12887 T12888 advmod previously,unknown
R2405 T12837 T12835 pobj enzymes,of
R2406 T12838 T12822 aux has,remained
R2407 T12839 T12822 acomp elusive,remained
R2408 T12840 T12822 punct .,remained
R2409 T12842 T12843 prep In,been
R2410 T12844 T12845 amod large,part
R2411 T12845 T12842 pobj part,In
R2412 T12846 T12843 punct ", ",been
R2413 T12847 T12843 nsubj this,been
R2414 T12848 T12843 aux has,been
R2415 T12849 T12843 prep due,been
R2416 T12850 T12849 pcomp to,due
R2417 T12888 T12889 amod unknown,isoforms
R2418 T12851 T12852 det the,lack
R2419 T12852 T12849 pobj lack,due
R2420 T12853 T12852 prep of,lack
R2421 T12854 T12853 pobj inhibitors,of
R2422 T12855 T12854 amod capable,inhibitors
R2423 T12856 T12855 prep of,capable
R2424 T12889 T12886 pobj isoforms,of
R2425 T12857 T12858 advmod specifically,regulating
R2426 T12858 T12856 pcomp regulating,of
R2427 T12859 T12858 advmod down,regulating
R2428 T12890 T12891 dep which,regulated
R2429 T12860 T12858 punct -,regulating
R2430 T12861 T12862 det the,calpains
R2431 T12862 T12858 dobj calpains,regulating
R2432 T12891 T12889 relcl regulated,isoforms
R2433 T12863 T12858 prep without,regulating
R2434 T12864 T12863 pcomp affecting,without
R2435 T12865 T12866 amod other,proteases
R2436 T12892 T12891 aux may,regulated
R2437 T12866 T12864 dobj proteases,affecting
R2438 T12867 T12843 punct .,been
R2439 T12893 T12891 auxpass be,regulated
R2440 T12869 T12870 prep In,complicated
R2441 T12894 T12891 advmod differently,regulated
R2442 T12871 T12872 det the,decade
R2443 T12872 T12869 pobj decade,In
R2444 T12873 T12872 amod past,decade
R2445 T12895 T12891 cc and,regulated
R2446 T12874 T12870 punct ", ",complicated
R2447 T12896 T12891 conj have,regulated
R2448 T12897 T12898 amod different,specificity
R2449 T12898 T12896 dobj specificity,have
R2450 T12899 T12898 compound substrate,specificity
R2451 T12981 T12979 nsubjpass subunit,required
R2452 T12900 T12870 punct .,complicated
R2453 T12982 T12981 amod small,subunit
R2454 T12983 T12979 auxpass is,required
R2455 T12902 T12903 compound Gene,targeting
R2456 T12984 T12979 prep for,required
R2457 T12985 T12986 preconj both,calpain
R2458 T12903 T12904 nsubj targeting,provided
R2459 T12905 T12903 prep in,targeting
R2460 T12986 T12984 pobj calpain,for
R2461 T12987 T12986 nmod μ,calpain
R2462 T12988 T12987 punct -,μ
R2463 T12989 T12987 cc and,μ
R2464 T12990 T12987 conj m,μ
R2465 T12991 T12986 punct -,calpain
R2466 T12992 T12974 punct ", ",supported
R2467 T12993 T12974 cc and,supported
R2468 T12994 T12995 advmod furthermore,suggested
R2469 T12906 T12905 pobj mice,in
R2470 T12995 T12974 conj suggested,supported
R2471 T12996 T12997 nummod four,possibilities
R2472 T12997 T12995 dobj possibilities,suggested
R2473 T12907 T12904 aux has,provided
R2474 T12998 T12997 prep regarding,possibilities
R2475 T12999 T13000 poss their,requirement
R2476 T13000 T12998 pobj requirement,regarding
R2477 T12908 T12909 det a,approach
R2478 T13001 T13000 prep for,requirement
R2479 T13002 T13003 amod embryonic,development
R2480 T13003 T13001 pobj development,for
R2481 T12909 T12904 dobj approach,provided
R2482 T13004 T12975 punct : ,required
R2483 T13005 T12975 meta 1,required
R2484 T13006 T13005 punct ),1
R2485 T12910 T12909 amod powerful,approach
R2486 T13007 T13008 det both,isoforms
R2487 T13008 T12975 nsubjpass isoforms,required
R2488 T12911 T12912 aux to,examine
R2489 T13009 T12975 auxpass were,required
R2490 T13010 T12975 punct ;,required
R2491 T13011 T13012 meta 2,required
R2492 T13012 T12975 conj required,required
R2493 T13013 T13011 punct ),2
R2494 T12912 T12909 advcl examine,approach
R2495 T13014 T13015 compound μ,calpain
R2496 T13015 T13012 nsubjpass calpain,required
R2497 T13016 T13015 punct -,calpain
R2498 T12913 T12914 det the,roles
R2499 T13017 T13012 auxpass was,required
R2500 T13018 T13012 punct ;,required
R2501 T13019 T13020 meta 3,required
R2502 T12914 T12912 dobj roles,examine
R2503 T13020 T13012 conj required,required
R2504 T13021 T13019 punct ),3
R2505 T13022 T13023 compound m,calpain
R2506 T12915 T12914 amod physiologic,roles
R2507 T13023 T13020 nsubjpass calpain,required
R2508 T13024 T13023 punct -,calpain
R2509 T13025 T13020 auxpass was,required
R2510 T12916 T12914 prep of,roles
R2511 T13026 T13020 punct ", ",required
R2512 T13027 T13020 cc or,required
R2513 T13028 T13029 meta 4,are
R2514 T13029 T13020 conj are,required
R2515 T13030 T13028 punct ),4
R2516 T13031 T13032 nmod μ,calpain
R2517 T13032 T13029 nsubj calpain,are
R2518 T13033 T13031 punct -,μ
R2519 T12917 T12918 amod individual,isoforms
R2520 T13034 T13031 cc and,μ
R2521 T13035 T13031 conj m,μ
R2522 T13036 T13032 punct -,calpain
R2523 T12918 T12916 pobj isoforms,of
R2524 T13037 T13029 acomp redundant,are
R2525 T13038 T13029 punct ", ",are
R2526 T13039 T13029 cc and,are
R2527 T12919 T12918 compound calpain,isoforms
R2528 T13040 T13041 nsubjpass one,required
R2529 T13041 T13029 conj required,are
R2530 T13042 T13040 cc or,one
R2531 T12920 T12904 punct .,provided
R2532 T13043 T13044 det the,other
R2533 T13044 T13045 nmod other,isoform
R2534 T13045 T13040 conj isoform,one
R2535 T12922 T12923 nsubjpass This,used
R2536 T13046 T13041 auxpass was,required
R2537 T13047 T12975 punct .,required
R2538 T13049 T13050 det These,options
R2539 T12924 T12923 auxpass was,used
R2540 T13050 T13051 nsubjpass options,narrowed
R2541 T13052 T13051 auxpass were,narrowed
R2542 T13053 T13051 prt down,narrowed
R2543 T13054 T13051 agent by,narrowed
R2544 T12925 T12923 advmod first,used
R2545 T13055 T13056 det the,observation
R2546 T13056 T13054 pobj observation,by
R2547 T13057 T13056 amod subsequent,observation
R2548 T13058 T13059 mark that,were
R2549 T12926 T12927 aux to,disrupt
R2550 T12927 T12923 advcl disrupt,used
R2551 T13059 T13056 acl were,observation
R2552 T12928 T12929 det the,gene
R2553 T13060 T13061 nmod Capn1,mice
R2554 T13061 T13059 nsubj mice,were
R2555 T13062 T13060 punct -,Capn1
R2556 T13063 T13060 punct /,Capn1
R2557 T12929 T12927 dobj gene,disrupt
R2558 T13064 T13060 punct -,Capn1
R2559 T13065 T13061 punct ", ",mice
R2560 T12930 T12929 compound Capn4,gene
R2561 T13066 T13067 dep which,lack
R2562 T13067 T13061 relcl lack,mice
R2563 T13068 T13069 det the,subunit
R2564 T13069 T13067 dobj subunit,lack
R2565 T13070 T13071 nmod μ,calpain
R2566 T13071 T13069 nmod calpain,subunit
R2567 T13072 T13071 punct -,calpain
R2568 T13073 T13069 amod catalytic,subunit
R2569 T13074 T13059 punct ", ",were
R2570 T13075 T13059 acomp healthy,were
R2571 T13076 T13075 cc and,healthy
R2572 T12931 T12923 punct ", ",used
R2573 T13077 T13075 conj fertile,healthy
R2574 T13078 T13059 punct ", ",were
R2575 T13079 T13080 mark although,observed
R2576 T12932 T12923 advcl encoding,used
R2577 T13080 T13059 advcl observed,were
R2578 T13081 T13082 nmod platelet,aggregation
R2579 T12933 T12934 det the,subunit
R2580 T13082 T13083 nmod aggregation,defects
R2581 T13083 T13080 nsubjpass defects,observed
R2582 T13084 T13082 cc and,aggregation
R2583 T13085 T13086 compound clot,retraction
R2584 T12934 T12932 dobj subunit,encoding
R2585 T13086 T13082 conj retraction,aggregation
R2586 T12935 T12934 amod small,subunit
R2587 T12936 T12934 amod regulatory,subunit
R2588 T12937 T12934 amod common,subunit
R2589 T13087 T13080 auxpass were,observed
R2590 T12938 T12937 prep to,common
R2591 T13088 T13089 punct [,33
R2592 T13089 T13059 parataxis 33,were
R2593 T13090 T13089 punct ],33
R2594 T12939 T12940 preconj both,calpain
R2595 T13091 T13051 punct .,narrowed
R2596 T12940 T12938 pobj calpain,to
R2597 T13093 T13094 prep At,left
R2598 T13095 T13096 det that,point
R2599 T12941 T12940 nmod μ,calpain
R2600 T13096 T13093 pobj point,At
R2601 T13097 T13094 punct ", ",left
R2602 T13098 T13094 nsubjpass we,left
R2603 T12942 T12941 punct -,μ
R2604 T13099 T13094 auxpass were,left
R2605 T13100 T13094 prep with,left
R2606 T13101 T13102 det the,possibilities
R2607 T12943 T12941 cc and,μ
R2608 T13102 T13100 pobj possibilities,with
R2609 T13103 T13102 amod last,possibilities
R2610 T13104 T13102 nummod two,possibilities
R2611 T12944 T12941 conj m,μ
R2612 T13105 T13106 mark that,required
R2613 T13106 T13102 advcl required,possibilities
R2614 T13107 T13108 det either,calpain
R2615 T13108 T13106 nsubjpass calpain,required
R2616 T13109 T13108 compound m,calpain
R2617 T13110 T13108 punct -,calpain
R2618 T13111 T13106 auxpass was,required
R2619 T13112 T13106 advmod specifically,required
R2620 T13113 T13106 prep during,required
R2621 T12945 T12940 punct -,calpain
R2622 T13114 T13113 pobj embryogenesis,during
R2623 T13115 T13106 punct ", ",required
R2624 T13116 T13106 cc or,required
R2625 T13117 T13118 mark that,was
R2626 T12946 T12923 punct .,used
R2627 T13118 T13106 conj was,required
R2628 T13119 T13120 det either,isoform
R2629 T13120 T13118 nsubj isoform,was
R2630 T13121 T13120 advmod alone,isoform
R2631 T13122 T13118 acomp sufficient,was
R2632 T12948 T12949 nummod Two,laboratories
R2633 T13123 T13122 prep for,sufficient
R2634 T13124 T13123 pcomp sustaining,for
R2635 T13125 T13126 amod embryonic,viability
R2636 T12949 T12951 nsubj laboratories,observed
R2637 T13126 T13124 dobj viability,sustaining
R2638 T13127 T13094 punct .,left
R2639 T13129 T13130 nsubj We,report
R2640 T12950 T12949 amod independent,laboratories
R2641 T13131 T13130 advmod here,report
R2642 T13132 T13133 mark that,die
R2643 T12952 T12953 amod embryonic,lethality
R2644 T13133 T13130 ccomp die,report
R2645 T13134 T13135 nmod Capn2,mice
R2646 T13135 T13133 nsubj mice,die
R2647 T13136 T13134 punct -,Capn2
R2648 T13137 T13134 punct /,Capn2
R2649 T13138 T13134 punct -,Capn2
R2650 T12953 T12951 dobj lethality,observed
R2651 T13139 T13135 punct ", ",mice
R2652 T13140 T13141 dep which,lack
R2653 T13141 T13135 relcl lack,mice
R2654 T12954 T12951 prep in,observed
R2655 T13142 T13143 det the,subunit
R2656 T13143 T13141 dobj subunit,lack
R2657 T13144 T13145 nmod m,calpain
R2658 T12955 T12956 compound Capn4,mice
R2659 T13145 T13143 nmod calpain,subunit
R2660 T13146 T13145 punct -,calpain
R2661 T13147 T13143 amod catalytic,subunit
R2662 T12956 T12954 pobj mice,in
R2663 T13148 T13133 punct ", ",die
R2664 T13149 T13133 prep at,die
R2665 T13150 T13151 det the,stage
R2666 T12957 T12956 compound knockout,mice
R2667 T13151 T13149 pobj stage,at
R2668 T13152 T13151 compound preimplantation,stage
R2669 T13153 T13151 prep of,stage
R2670 T13154 T13153 pobj development,of
R2671 T13155 T13130 punct .,report
R2672 T12958 T12951 punct ", ",observed
R2673 T13157 T13158 det This,observation
R2674 T12959 T12960 dep albeit,at
R2675 T12960 T12951 prep at,observed
R2676 T13158 T13159 nsubj observation,allows
R2677 T12961 T12962 amod different,stages
R2678 T13160 T13161 nsubj us,conclude
R2679 T13161 T13159 ccomp conclude,allows
R2680 T12962 T12960 pobj stages,at
R2681 T13162 T13161 aux to,conclude
R2682 T13163 T13161 advmod now,conclude
R2683 T13164 T13165 mark that,required
R2684 T12963 T12962 prep of,stages
R2685 T13165 T13161 ccomp required,conclude
R2686 T13166 T13167 compound m,calpain
R2687 T12964 T12963 pobj development,of
R2688 T13167 T13165 nsubjpass calpain,required
R2689 T13168 T13167 punct -,calpain
R2690 T13169 T13165 auxpass is,required
R2691 T12965 T12966 punct [,32
R2692 T13170 T13165 advmod specifically,required
R2693 T13171 T13165 prep during,required
R2694 T13172 T13171 pobj embryogenesis,during
R2695 T12966 T12951 parataxis 32,observed
R2696 T13173 T13159 punct .,allows
R2697 T12967 T12966 nummod 31,32
R2698 T13175 T13176 mark Since,expected
R2699 T13176 T13185 advcl expected,is
R2700 T13177 T13178 amod homozygous,disruption
R2701 T12968 T12966 punct ",",32
R2702 T13178 T13176 nsubjpass disruption,expected
R2703 T12969 T12966 punct ],32
R2704 T13179 T13178 prep of,disruption
R2705 T13180 T13181 det the,gene
R2706 T13181 T13179 pobj gene,of
R2707 T12970 T12951 punct .,observed
R2708 T13182 T13181 compound Capn4,gene
R2709 T13183 T13176 auxpass was,expected
R2710 T12972 T12973 det These,observations
R2711 T13184 T13176 advmod also,expected
R2712 T13186 T13187 aux to,abolish
R2713 T13187 T13176 xcomp abolish,expected
R2714 T12973 T12974 nsubj observations,supported
R2715 T13188 T13189 compound m,calpain
R2716 T13189 T13191 compound calpain,activity
R2717 T13190 T13189 punct -,calpain
R2718 T12974 T12975 ccomp supported,required
R2719 T13191 T13187 dobj activity,abolish
R2720 T13192 T13185 punct ", ",is
R2721 T12976 T12977 det the,hypothesis
R2722 T13193 T13194 det this,result
R2723 T13194 T13185 nsubj result,is
R2724 T12977 T12974 dobj hypothesis,supported
R2725 T13195 T13185 prep in,is
R2726 T12978 T12979 mark that,required
R2727 T13196 T13195 pobj agreement,in
R2728 T13197 T13196 prep with,agreement
R2729 T13198 T13199 det the,phenotype
R2730 T13199 T13197 pobj phenotype,with
R2731 T13200 T13199 acl presented,phenotype
R2732 T12979 T12977 acl required,hypothesis
R2733 T13201 T13200 agent by,presented
R2734 T13202 T13201 pobj one,by
R2735 T13203 T13202 prep of,one
R2736 T12980 T12981 det the,subunit
R2737 T13204 T13205 det the,lines
R2738 T13205 T13203 pobj lines,of
R2739 T13299 T13300 det the,cassette
R2740 T13206 T13205 nummod two,lines
R2741 T13207 T13208 npadvmod Capn4,targeted
R2742 T13208 T13205 amod targeted,lines
R2743 T13209 T13210 prep in,observed
R2744 T13300 T13298 pobj cassette,of
R2745 T13210 T13205 relcl observed,lines
R2746 T13211 T13209 pobj which,in
R2747 T13212 T13213 compound preimplantation,lethality
R2748 T13301 T13302 compound PGK,Neo
R2749 T13213 T13210 nsubjpass lethality,observed
R2750 T13214 T13213 prep of,lethality
R2751 T13215 T13216 amod null,embryos
R2752 T13216 T13214 pobj embryos,of
R2753 T13302 T13300 compound Neo,cassette
R2754 T13217 T13210 auxpass was,observed
R2755 T13218 T13210 advmod also,observed
R2756 T13219 T13220 punct [,32
R2757 T13303 T13302 punct -,Neo
R2758 T13304 T13297 prep into,insertion
R2759 T13220 T13210 parataxis 32,observed
R2760 T13305 T13306 det the,middle
R2761 T13221 T13220 punct ],32
R2762 T13222 T13185 punct .,is
R2763 T13306 T13304 pobj middle,into
R2764 T13224 T13225 det The,survival
R2765 T13307 T13306 prep of,middle
R2766 T13225 T13226 nsubj survival,is
R2767 T13308 T13307 pobj exon,of
R2768 T13227 T13225 prep of,survival
R2769 T13228 T13229 nmod Capn4,embryos
R2770 T13229 T13227 pobj embryos,of
R2771 T13230 T13228 punct -,Capn4
R2772 T13231 T13228 punct /,Capn4
R2773 T13232 T13228 punct -,Capn4
R2774 T13233 T13225 prep from,survival
R2775 T13309 T13308 nummod 9,exon
R2776 T13234 T13235 det the,line
R2777 T13235 T13233 pobj line,from
R2778 T13236 T13235 amod original,line
R2779 T13310 T13297 punct ", ",insertion
R2780 T13237 T13235 amod targeted,line
R2781 T13238 T13235 acl reported,line
R2782 T13311 T13312 dep which,caused
R2783 T13239 T13238 agent by,reported
R2784 T13240 T13239 pobj Arthur,by
R2785 T13241 T13240 cc and,Arthur
R2786 T13312 T13297 relcl caused,insertion
R2787 T13242 T13240 conj colleagues,Arthur
R2788 T13313 T13312 dobj truncation,caused
R2789 T13314 T13313 prep of,truncation
R2790 T13243 T13233 prep to,from
R2791 T13244 T13243 pobj mid-gestation,to
R2792 T13315 T13316 det the,protein
R2793 T13245 T13246 advmod more,difficult
R2794 T13246 T13226 acomp difficult,is
R2795 T13316 T13314 pobj protein,of
R2796 T13247 T13248 aux to,reconcile
R2797 T13248 T13246 advcl reconcile,difficult
R2798 T13249 T13250 punct [,31
R2799 T13317 T13318 punct [,31
R2800 T13250 T13226 parataxis 31,is
R2801 T13251 T13250 punct ],31
R2802 T13318 T13312 parataxis 31,caused
R2803 T13252 T13226 punct .,is
R2804 T13254 T13255 prep In,seems
R2805 T13319 T13318 punct ],31
R2806 T13256 T13254 pobj retrospect,In
R2807 T13320 T13280 punct .,involved
R2808 T13257 T13255 punct ", ",seems
R2809 T13258 T13255 nsubj it,seems
R2810 T13259 T13255 oprd likely,seems
R2811 T13322 T13323 det This,strategy
R2812 T13260 T13261 mark that,represents
R2813 T13261 T13255 ccomp represents,seems
R2814 T13262 T13263 det the,line
R2815 T13263 T13261 nsubj line,represents
R2816 T13323 T13324 nsubjpass strategy,based
R2817 T13264 T13263 amod latter,line
R2818 T13265 T13266 det a,state
R2819 T13325 T13324 auxpass was,based
R2820 T13266 T13261 dobj state,represents
R2821 T13267 T13266 amod hypomorphic,state
R2822 T13268 T13266 punct ", ",state
R2823 T13269 T13270 advmod rather,than
R2824 T13270 T13266 cc than,state
R2825 T13271 T13272 det a,null
R2826 T13272 T13266 conj null,state
R2827 T13273 T13272 amod true,null
R2828 T13274 T13255 punct .,seems
R2829 T13326 T13324 prep upon,based
R2830 T13276 T13277 det The,strategy
R2831 T13277 T13280 nsubj strategy,involved
R2832 T13327 T13328 amod previous,studies
R2833 T13278 T13277 compound Capn4,strategy
R2834 T13279 T13277 compound targeting,strategy
R2835 T13328 T13326 pobj studies,upon
R2836 T13281 T13277 acl employed,strategy
R2837 T13282 T13281 agent by,employed
R2838 T13283 T13282 pobj Arthur,by
R2839 T13284 T13283 cc and,Arthur
R2840 T13285 T13283 conj colleagues,Arthur
R2841 T13329 T13330 compound structure,function
R2842 T13286 T13280 xcomp disrupting,involved
R2843 T13287 T13288 det the,terminus
R2844 T13288 T13286 dobj terminus,disrupting
R2845 T13330 T13328 compound function,studies
R2846 T13289 T13288 compound C,terminus
R2847 T13290 T13288 punct -,terminus
R2848 T13291 T13288 prep of,terminus
R2849 T13292 T13293 det the,subunit
R2850 T13331 T13330 punct /,function
R2851 T13293 T13291 pobj subunit,of
R2852 T13294 T13293 nmod calpain,subunit
R2853 T13332 T13328 acl showing,studies
R2854 T13295 T13293 amod small,subunit
R2855 T13296 T13286 agent by,disrupting
R2856 T13297 T13296 pobj insertion,by
R2857 T13333 T13334 mark that,abolished
R2858 T13298 T13297 prep of,insertion
R2859 T13334 T13332 ccomp abolished,showing
R2860 T13335 T13334 nsubj excision,abolished
R2861 T13336 T13335 prep of,excision
R2862 T13405 T13406 det the,time
R2863 T13406 T13404 pobj time,in
R2864 T13337 T13338 det the,acid
R2865 T13407 T13406 prep of,time
R2866 T13408 T13407 pobj lethality,of
R2867 T13409 T13406 prep of,time
R2868 T13338 T13336 pobj acid,of
R2869 T13410 T13411 det these,lines
R2870 T13339 T13340 npadvmod C,terminal
R2871 T13340 T13338 amod terminal,acid
R2872 T13411 T13409 pobj lines,of
R2873 T13341 T13340 punct -,terminal
R2874 T13412 T13411 nummod two,lines
R2875 T13413 T13411 amod targeted,lines
R2876 T13414 T13411 compound Capn4,lines
R2877 T13415 T13401 aux can,explained
R2878 T13416 T13401 auxpass be,explained
R2879 T13417 T13401 agent by,explained
R2880 T13418 T13419 amod different,extents
R2881 T13342 T13338 nummod 25,acid
R2882 T13419 T13417 pobj extents,by
R2883 T13420 T13419 prep of,extents
R2884 T13421 T13420 pobj disruption,of
R2885 T13343 T13338 compound amino,acid
R2886 T13422 T13397 punct .,seems
R2887 T13344 T13338 nmod residues,acid
R2888 T13424 T13425 det The,allele
R2889 T13425 T13427 nsubj allele,represents
R2890 T13345 T13338 prep of,acid
R2891 T13426 T13425 compound Zimmerman,allele
R2892 T13346 T13347 det the,subunit
R2893 T13428 T13427 advmod probably,represents
R2894 T13429 T13430 det a,genotype
R2895 T13430 T13427 dobj genotype,represents
R2896 T13347 T13345 pobj subunit,of
R2897 T13431 T13430 amod true,genotype
R2898 T13432 T13430 amod null,genotype
R2899 T13433 T13434 mark while,is
R2900 T13348 T13347 amod small,subunit
R2901 T13434 T13427 advcl is,represents
R2902 T13435 T13436 det the,allele
R2903 T13436 T13434 nsubj allele,is
R2904 T13349 T13350 compound calpain,activity
R2905 T13437 T13436 compound Arthur,allele
R2906 T13438 T13434 advmod likely,is
R2907 T13439 T13440 det a,mutation
R2908 T13350 T13334 dobj activity,abolished
R2909 T13440 T13434 attr mutation,is
R2910 T13441 T13440 amod hypomorphic,mutation
R2911 T13442 T13427 punct .,represents
R2912 T13351 T13352 advmod when,co-expressed
R2913 T13444 T13445 amod Alternate,strategies
R2914 T13352 T13334 advcl co-expressed,abolished
R2915 T13445 T13447 nsubjpass strategies,shown
R2916 T13446 T13445 compound targeting,strategies
R2917 T13353 T13352 prep with,co-expressed
R2918 T13448 T13447 aux have,shown
R2919 T13449 T13447 auxpass been,shown
R2920 T13450 T13451 aux to,yield
R2921 T13354 T13355 det the,subunit
R2922 T13451 T13447 advcl yield,shown
R2923 T13452 T13453 amod different,phenotypes
R2924 T13453 T13451 dobj phenotypes,yield
R2925 T13355 T13353 pobj subunit,with
R2926 T13454 T13451 prep in,yield
R2927 T13455 T13456 compound gene,disruption
R2928 T13456 T13457 compound disruption,studies
R2929 T13457 T13454 pobj studies,in
R2930 T13458 T13447 punct .,shown
R2931 T13460 T13461 prep For,used
R2932 T13356 T13357 nmod m,calpain
R2933 T13462 T13460 pobj example,For
R2934 T13463 T13461 punct ", ",used
R2935 T13464 T13465 nummod three,strategies
R2936 T13465 T13461 nsubjpass strategies,used
R2937 T13466 T13465 amod different,strategies
R2938 T13357 T13355 nmod calpain,subunit
R2939 T13467 T13465 compound targeting,strategies
R2940 T13468 T13461 auxpass were,used
R2941 T13469 T13461 advmod independently,used
R2942 T13358 T13357 punct -,calpain
R2943 T13470 T13471 aux to,disrupt
R2944 T13471 T13461 advcl disrupt,used
R2945 T13359 T13355 amod large,subunit
R2946 T13472 T13473 det the,gene
R2947 T13473 T13471 dobj gene,disrupt
R2948 T13474 T13475 amod mixed,leukemia
R2949 T13475 T13473 nmod leukemia,gene
R2950 T13360 T13352 prep in,co-expressed
R2951 T13476 T13475 nmod lineage,leukemia
R2952 T13477 T13475 punct (,leukemia
R2953 T13361 T13362 compound E.,coli
R2954 T13478 T13475 appos Mll,leukemia
R2955 T13479 T13473 punct ),gene
R2956 T13480 T13461 punct .,used
R2957 T13362 T13360 pobj coli,in
R2958 T13482 T13483 prep In,perished
R2959 T13484 T13485 det all,studies
R2960 T13363 T13364 punct [,34
R2961 T13485 T13482 pobj studies,In
R2962 T13486 T13485 nummod three,studies
R2963 T13487 T13483 punct ", ",perished
R2964 T13364 T13334 parataxis 34,abolished
R2965 T13488 T13489 amod homozygous,null
R2966 T13489 T13490 amod null,embryos
R2967 T13490 T13483 nsubj embryos,perished
R2968 T13365 T13364 punct ],34
R2969 T13491 T13483 prep during,perished
R2970 T13492 T13491 pobj embryogenesis,during
R2971 T13366 T13324 punct .,based
R2972 T13493 T13491 punct ", ",during
R2973 T13494 T13491 cc but,during
R2974 T13495 T13491 conj at,during
R2975 T13496 T13497 amod different,stages
R2976 T13368 T13369 prep In,involved
R2977 T13497 T13495 pobj stages,at
R2978 T13498 T13499 punct (,E14.5
R2979 T13499 T13497 parataxis E14.5,stages
R2980 T13500 T13499 nmod E0.5,E14.5
R2981 T13501 T13499 punct ", ",E14.5
R2982 T13370 T13368 pobj contrast,In
R2983 T13502 T13499 nmod E10.5,E14.5
R2984 T13503 T13499 punct ", ",E14.5
R2985 T13504 T13499 punct ),E14.5
R2986 T13505 T13506 punct [,35
R2987 T13506 T13483 parataxis 35,perished
R2988 T13371 T13369 punct ", ",involved
R2989 T13507 T13506 punct ],35
R2990 T13508 T13483 punct .,perished
R2991 T13372 T13373 det the,strategy
R2992 T13510 T13511 det The,variation
R2993 T13373 T13369 nsubj strategy,involved
R2994 T13374 T13373 nmod Capn4,strategy
R2995 T13375 T13373 amod targeting,strategy
R2996 T13511 T13512 nsubjpass variation,attributed
R2997 T13376 T13373 acl employed,strategy
R2998 T13513 T13511 prep in,variation
R2999 T13377 T13376 agent by,employed
R3000 T13514 T13513 pobj phenotype,in
R3001 T13515 T13512 auxpass was,attributed
R3002 T13516 T13512 prep to,attributed
R3003 T13517 T13518 det the,differences
R3004 T13378 T13377 pobj Zimmerman,by
R3005 T13518 T13516 pobj differences,to
R3006 T13519 T13518 prep in,differences
R3007 T13520 T13519 pobj degree,in
R3008 T13379 T13378 cc and,Zimmerman
R3009 T13521 T13520 prep of,degree
R3010 T13522 T13521 pobj function,of
R3011 T13523 T13520 prep of,degree
R3012 T13380 T13378 conj colleagues,Zimmerman
R3013 T13524 T13525 det the,proteins
R3014 T13525 T13523 pobj proteins,of
R3015 T13526 T13525 amod truncated,proteins
R3016 T13381 T13382 det a,deletion
R3017 T13527 T13525 acl produced,proteins
R3018 T13528 T13527 prep from,produced
R3019 T13529 T13530 det the,alleles
R3020 T13382 T13369 dobj deletion,involved
R3021 T13530 T13528 pobj alleles,from
R3022 T13531 T13530 compound mutant,alleles
R3023 T13532 T13512 punct .,attributed
R3024 T13383 T13384 advmod much,more
R3025 T13534 T13535 det A,effect
R3026 T13535 T13537 nsubj effect,be
R3027 T13384 T13385 advmod more,extensive
R3028 T13536 T13535 amod similar,effect
R3029 T13538 T13537 aux might,be
R3030 T13385 T13382 amod extensive,deletion
R3031 T13539 T13537 prep at,be
R3032 T13540 T13539 pobj work,at
R3033 T13541 T13537 prep in,be
R3034 T13542 T13543 det the,lines
R3035 T13543 T13541 pobj lines,in
R3036 T13544 T13543 nummod two,lines
R3037 T13386 T13382 prep of,deletion
R3038 T13545 T13546 npadvmod Capn4,transgenic
R3039 T13546 T13543 amod transgenic,lines
R3040 T13547 T13537 punct .,be
R3041 T13387 T13388 nmod exons,4
R3042 T13549 T13550 nsubjpass Efforts,made
R3043 T13388 T13386 pobj 4,of
R3044 T13551 T13550 auxpass were,made
R3045 T13552 T13553 aux to,detect
R3046 T13553 T13550 advcl detect,made
R3047 T13389 T13388 prep through,4
R3048 T13554 T13553 dobj transcripts,detect
R3049 T13555 T13554 cc or,transcripts
R3050 T13390 T13389 pobj 8,through
R3051 T13556 T13557 compound calpain,activities
R3052 T13557 T13554 conj activities,transcripts
R3053 T13391 T13392 punct [,32
R3054 T13558 T13554 acl derived,transcripts
R3055 T13559 T13558 prep from,derived
R3056 T13560 T13561 det the,allele
R3057 T13561 T13559 pobj allele,from
R3058 T13392 T13369 parataxis 32,involved
R3059 T13562 T13563 compound Arthur,Capn4
R3060 T13563 T13561 compound Capn4,allele
R3061 T13564 T13550 punct .,made
R3062 T13393 T13392 punct ],32
R3063 T13566 T13567 det This,allele
R3064 T13567 T13568 nsubj allele,gave
R3065 T13394 T13369 punct .,involved
R3066 T13569 T13568 dobj rise,gave
R3067 T13396 T13397 nsubj It,seems
R3068 T13570 T13568 prep to,gave
R3069 T13571 T13572 amod multiple,species
R3070 T13572 T13570 pobj species,to
R3071 T13573 T13572 compound mRNA,species
R3072 T13398 T13397 advmod now,seems
R3073 T13574 T13572 punct ", ",species
R3074 T13575 T13572 amod detectable,species
R3075 T13576 T13575 prep by,detectable
R3076 T13577 T13578 compound RT,PCR
R3077 T13399 T13397 oprd probable,seems
R3078 T13578 T13576 pobj PCR,by
R3079 T13579 T13578 punct -,PCR
R3080 T13580 T13568 punct ", ",gave
R3081 T13400 T13401 mark that,explained
R3082 T13581 T13568 advcl reading,gave
R3083 T13582 T13581 advmod through,reading
R3084 T13583 T13581 prep from,reading
R3085 T13584 T13585 det the,half
R3086 T13585 T13583 pobj half,from
R3087 T13401 T13397 ccomp explained,seems
R3088 T13586 T13585 amod first,half
R3089 T13587 T13585 prep of,half
R3090 T13588 T13587 pobj exon,of
R3091 T13402 T13403 det the,difference
R3092 T13403 T13401 nsubjpass difference,explained
R3093 T13589 T13588 nummod 9,exon
R3094 T13590 T13583 prep to,from
R3095 T13404 T13403 prep in,difference
R3096 T13591 T13592 advmod at,two
R3097 T13592 T13594 nummod two,sites
R3098 T13593 T13592 advmod least,two
R3099 T13617 T13615 amod small,subunits
R3100 T13594 T13590 pobj sites,to
R3101 T13595 T13594 amod different,sites
R3102 T13596 T13594 amod cryptic,sites
R3103 T13597 T13594 compound splice,sites
R3104 T13618 T13610 prep with,give
R3105 T13598 T13594 prep in,sites
R3106 T13599 T13600 det the,sequence
R3107 T13619 T13620 quantmod 10,30
R3108 T13600 T13598 pobj sequence,in
R3109 T13601 T13600 compound PGK,sequence
R3110 T13602 T13600 compound promoter,sequence
R3111 T13620 T13622 nummod 30,acids
R3112 T13603 T13604 punct [,31
R3113 T13604 T13581 parataxis 31,reading
R3114 T13605 T13604 punct ],31
R3115 T13621 T13620 punct –,30
R3116 T13606 T13568 punct .,gave
R3117 T13608 T13609 det These,transcripts
R3118 T13622 T13618 pobj acids,with
R3119 T13609 T13610 nsubj transcripts,give
R3120 T13611 T13610 aux could,give
R3121 T13623 T13622 amod inappropriate,acids
R3122 T13612 T13610 dobj rise,give
R3123 T13613 T13610 prep to,give
R3124 T13614 T13615 amod defective,subunits
R3125 T13624 T13625 npadvmod C,terminal
R3126 T13615 T13613 pobj subunits,to
R3127 T13625 T13622 amod terminal,acids
R3128 T13626 T13625 punct -,terminal
R3129 T13616 T13615 nmod calpain,subunits
R3130 T13627 T13622 punct ", ",acids
R3131 T13628 T13629 dep which,be
R3132 T13629 T13622 relcl be,acids
R3133 T13723 T13721 pobj hands,in
R3134 T13630 T13629 aux might,be
R3135 T13724 T13725 advmod when,expressed
R3136 T13725 T13719 advcl expressed,observed
R3137 T13726 T13727 nmod calpain,subunits
R3138 T13727 T13725 nsubjpass subunits,expressed
R3139 T13728 T13727 amod large,subunits
R3140 T13729 T13725 auxpass were,expressed
R3141 T13631 T13629 acomp sufficient,be
R3142 T13730 T13725 advmod alone,expressed
R3143 T13731 T13732 preconj either,in
R3144 T13732 T13725 prep in,expressed
R3145 T13632 T13633 aux to,support
R3146 T13733 T13734 compound E.,coli
R3147 T13734 T13732 pobj coli,in
R3148 T13735 T13732 cc or,in
R3149 T13633 T13631 xcomp support,sufficient
R3150 T13736 T13732 conj in,in
R3151 T13737 T13738 amod mammalian,cells
R3152 T13634 T13635 det a,level
R3153 T13738 T13736 pobj cells,in
R3154 T13739 T13740 punct [,36
R3155 T13740 T13725 parataxis 36,expressed
R3156 T13635 T13633 dobj level,support
R3157 T13741 T13740 punct ],36
R3158 T13742 T13655 punct .,suggested
R3159 T13636 T13635 amod low,level
R3160 T13744 T13745 det The,timing
R3161 T13745 T13747 nsubj timing,be
R3162 T13637 T13635 prep of,level
R3163 T13746 T13745 amod different,timing
R3164 T13638 T13639 compound calpain,activity
R3165 T13748 T13745 prep of,timing
R3166 T13749 T13748 pobj lethality,of
R3167 T13750 T13745 prep in,timing
R3168 T13639 T13637 pobj activity,of
R3169 T13751 T13752 det the,knockouts
R3170 T13752 T13750 pobj knockouts,in
R3171 T13753 T13752 compound Capn4,knockouts
R3172 T13640 T13610 punct .,give
R3173 T13754 T13747 aux might,be
R3174 T13755 T13747 advmod also,be
R3175 T13756 T13757 det a,consequence
R3176 T13642 T13643 advmod However,give
R3177 T13643 T13655 ccomp give,suggested
R3178 T13757 T13747 attr consequence,be
R3179 T13758 T13757 prep of,consequence
R3180 T13644 T13643 punct ", ",give
R3181 T13759 T13760 det the,backgrounds
R3182 T13760 T13758 pobj backgrounds,of
R3183 T13761 T13760 amod different,backgrounds
R3184 T13645 T13643 nsubj expression,give
R3185 T13762 T13760 amod genetic,backgrounds
R3186 T13763 T13760 prep of,backgrounds
R3187 T13764 T13765 det the,lines
R3188 T13765 T13763 pobj lines,of
R3189 T13766 T13765 nummod two,lines
R3190 T13646 T13645 prep of,expression
R3191 T13767 T13765 amod transgenic,lines
R3192 T13768 T13757 punct ", ",consequence
R3193 T13769 T13770 dep which,observed
R3194 T13647 T13646 pobj calpains,of
R3195 T13770 T13757 relcl observed,consequence
R3196 T13771 T13770 aux has,observed
R3197 T13772 T13770 auxpass been,observed
R3198 T13648 T13647 prep with,calpains
R3199 T13773 T13774 aux to,influence
R3200 T13774 T13770 advcl influence,observed
R3201 T13649 T13650 det these,subunits
R3202 T13775 T13776 det the,phenotype
R3203 T13776 T13774 dobj phenotype,influence
R3204 T13777 T13776 prep of,phenotype
R3205 T13650 T13648 pobj subunits,with
R3206 T13778 T13779 amod transgenic,mice
R3207 T13779 T13777 pobj mice,of
R3208 T13651 T13650 amod modified,subunits
R3209 T13780 T13774 prep on,influence
R3210 T13781 T13782 det a,number
R3211 T13782 T13780 pobj number,on
R3212 T13652 T13650 amod small,subunits
R3213 T13783 T13782 prep of,number
R3214 T13784 T13783 pobj occasions,of
R3215 T13785 T13786 punct [,37
R3216 T13653 T13643 aux did,give
R3217 T13786 T13774 parataxis 37,influence
R3218 T13787 T13786 punct ],37
R3219 T13654 T13643 neg not,give
R3220 T13788 T13747 punct .,be
R3221 T13790 T13791 nsubj One,been
R3222 T13656 T13643 dobj rise,give
R3223 T13792 T13790 prep of,One
R3224 T13793 T13794 det the,questions
R3225 T13794 T13792 pobj questions,of
R3226 T13657 T13643 prep to,give
R3227 T13795 T13794 amod enduring,questions
R3228 T13796 T13794 prep in,questions
R3229 T13797 T13798 compound calpain,research
R3230 T13658 T13659 det any,activity
R3231 T13798 T13796 pobj research,in
R3232 T13799 T13791 aux has,been
R3233 T13800 T13801 mark whether,possess
R3234 T13659 T13657 pobj activity,to
R3235 T13801 T13791 ccomp possess,been
R3236 T13802 T13803 det the,isoforms
R3237 T13803 T13801 nsubj isoforms,possess
R3238 T13660 T13659 amod detectable,activity
R3239 T13804 T13803 nummod two,isoforms
R3240 T13805 T13803 amod ubiquitous,isoforms
R3241 T13806 T13803 punct ", ",isoforms
R3242 T13807 T13808 nmod μ,calpain
R3243 T13808 T13803 appos calpain,isoforms
R3244 T13661 T13659 compound calpain,activity
R3245 T13809 T13807 punct -,μ
R3246 T13810 T13807 cc and,μ
R3247 T13811 T13807 conj m,μ
R3248 T13812 T13808 punct -,calpain
R3249 T13662 T13663 advmod when,expressed
R3250 T13813 T13801 punct ", ",possess
R3251 T13814 T13815 amod distinct,roles
R3252 T13663 T13643 advcl expressed,give
R3253 T13815 T13801 dobj roles,possess
R3254 T13816 T13817 advmod in,vivo
R3255 T13817 T13815 amod vivo,roles
R3256 T13664 T13663 prep in,expressed
R3257 T13818 T13791 punct .,been
R3258 T13820 T13821 det The,enzymes
R3259 T13665 T13666 compound E.,coli
R3260 T13821 T13823 nsubj enzymes,share
R3261 T13822 T13821 nummod two,enzymes
R3262 T13666 T13664 pobj coli,in
R3263 T13824 T13825 nummod 62,%
R3264 T13825 T13826 compound %,identity
R3265 T13667 T13643 punct ", ",give
R3266 T13826 T13823 dobj identity,share
R3267 T13827 T13826 compound sequence,identity
R3268 T13828 T13823 cc and,share
R3269 T13668 T13669 mark although,has
R3270 T13669 T13643 advcl has,give
R3271 T13670 T13671 poss their,functionality
R3272 T13829 T13823 conj are,share
R3273 T13830 T13831 advmod very,similar
R3274 T13671 T13669 nsubj functionality,has
R3275 T13831 T13829 acomp similar,are
R3276 T13832 T13829 prep in,are
R3277 T13833 T13834 poss their,structure
R3278 T13672 T13671 prep in,functionality
R3279 T13834 T13832 pobj structure,in
R3280 T13835 T13834 cc and,structure
R3281 T13673 T13674 amod mammalian,cells
R3282 T13836 T13834 conj biochemistry,structure
R3283 T13837 T13823 punct .,share
R3284 T13674 T13672 pobj cells,in
R3285 T13839 T13840 advmod Notably,cleave
R3286 T13675 T13669 advmod yet,has
R3287 T13841 T13840 punct ", ",cleave
R3288 T13842 T13840 nsubj they,cleave
R3289 T13843 T13840 advmod essentially,cleave
R3290 T13676 T13677 aux to,determined
R3291 T13677 T13669 xcomp determined,has
R3292 T13844 T13845 det the,set
R3293 T13845 T13840 dobj set,cleave
R3294 T13678 T13677 auxpass be,determined
R3295 T13846 T13845 amod same,set
R3296 T13847 T13845 prep of,set
R3297 T13848 T13847 pobj substrates,of
R3298 T13679 T13680 punct (,J.S.
R3299 T13849 T13850 advmod in,vitro
R3300 T13850 T13840 advmod vitro,cleave
R3301 T13680 T13669 meta J.S.,has
R3302 T13851 T13840 punct ", ",cleave
R3303 T13852 T13840 advcl suggesting,cleave
R3304 T13681 T13680 nmod Elce,J.S.
R3305 T13853 T13854 mark that,have
R3306 T13854 T13852 ccomp have,suggesting
R3307 T13855 T13854 nsubj they,have
R3308 T13682 T13680 punct ", ",J.S.
R3309 T13856 T13857 det the,potential
R3310 T13857 T13854 dobj potential,have
R3311 T13683 T13680 amod unpublished,J.S.
R3312 T13858 T13859 aux to,carry
R3313 T13859 T13857 acl carry,potential
R3314 T13860 T13859 prt out,carry
R3315 T13684 T13680 nmod work,J.S.
R3316 T13861 T13862 det the,functions
R3317 T13862 T13859 dobj functions,carry
R3318 T13863 T13862 amod same,functions
R3319 T13685 T13680 punct ),J.S.
R3320 T13864 T13865 advmod in,vivo
R3321 T13865 T13859 advmod vivo,carry
R3322 T13686 T13655 nsubjpass It,suggested
R3323 T13866 T13840 punct .,cleave
R3324 T13687 T13655 aux has,suggested
R3325 T13868 T13869 prep On,is
R3326 T13688 T13655 advmod also,suggested
R3327 T13870 T13871 det the,hand
R3328 T13871 T13868 pobj hand,On
R3329 T13872 T13871 amod other,hand
R3330 T13689 T13655 auxpass been,suggested
R3331 T13873 T13869 punct ", ",is
R3332 T13874 T13875 mark since,require
R3333 T13875 T13869 advcl require,is
R3334 T13690 T13691 mark that,provide
R3335 T13876 T13875 nsubj they,require
R3336 T13877 T13878 amod different,amounts
R3337 T13878 T13875 dobj amounts,require
R3338 T13691 T13655 ccomp provide,suggested
R3339 T13879 T13878 prep of,amounts
R3340 T13880 T13879 pobj Ca2,of
R3341 T13881 T13880 punct +,Ca2
R3342 T13692 T13693 nmod calpain,subunits
R3343 T13882 T13875 prep for,require
R3344 T13883 T13884 nmod in,vitro
R3345 T13884 T13885 nmod vitro,activation
R3346 T13885 T13882 pobj activation,for
R3347 T13886 T13869 punct ", ",is
R3348 T13693 T13691 nsubj subunits,provide
R3349 T13887 T13869 nsubj it,is
R3350 T13888 T13869 acomp possible,is
R3351 T13889 T13890 mark that,regulated
R3352 T13694 T13693 amod large,subunits
R3353 T13890 T13869 ccomp regulated,is
R3354 T13891 T13892 det the,isoforms
R3355 T13695 T13693 advmod alone,subunits
R3356 T13892 T13890 nsubjpass isoforms,regulated
R3357 T13893 T13892 nummod two,isoforms
R3358 T13696 T13691 aux might,provide
R3359 T13894 T13890 auxpass are,regulated
R3360 T13895 T13890 advmod differentially,regulated
R3361 T13896 T13890 prep inside,regulated
R3362 T13697 T13698 det some,activity
R3363 T13897 T13896 pobj cells,inside
R3364 T13898 T13869 punct .,is
R3365 T13698 T13691 dobj activity,provide
R3366 T13900 T13901 nsubj It,is
R3367 T13699 T13691 prep in,provide
R3368 T13902 T13901 advmod now,is
R3369 T13903 T13901 acomp clear,is
R3370 T13904 T13901 punct ", ",is
R3371 T13700 T13701 amod eukaryotic,cells
R3372 T13905 T13901 prep from,is
R3373 T13906 T13907 det the,work
R3374 T13907 T13905 pobj work,from
R3375 T13701 T13699 pobj cells,in
R3376 T13908 T13909 compound gene,targeting
R3377 T13909 T13907 compound targeting,work
R3378 T13910 T13907 acl done,work
R3379 T13702 T13691 punct ", ",provide
R3380 T13911 T13910 prep in,done
R3381 T13912 T13911 pobj mice,in
R3382 T13703 T13704 mark although,appears
R3383 T13913 T13901 punct ", ",is
R3384 T13914 T13915 mark that,have
R3385 T13915 T13901 ccomp have,is
R3386 T13704 T13691 advcl appears,provide
R3387 T13916 T13917 nmod μ,calpain
R3388 T13917 T13915 nsubj calpain,have
R3389 T13918 T13916 punct -,μ
R3390 T13705 T13706 det the,knockout
R3391 T13919 T13916 cc and,μ
R3392 T13920 T13916 conj m,μ
R3393 T13921 T13917 punct -,calpain
R3394 T13706 T13704 nsubj knockout,appears
R3395 T13922 T13923 det some,roles
R3396 T13923 T13915 dobj roles,have
R3397 T13924 T13923 amod distinct,roles
R3398 T13925 T13923 amod physiological,roles
R3399 T13926 T13915 punct ", ",have
R3400 T13707 T13706 nmod Zimmerman,knockout
R3401 T13927 T13928 advmod at,least
R3402 T13928 T13929 advmod least,during
R3403 T13708 T13709 advmod et,al.
R3404 T13929 T13915 prep during,have
R3405 T13709 T13707 advmod al.,Zimmerman
R3406 T13930 T13929 pobj embryogenesis,during
R3407 T13931 T13901 punct .,is
R3408 T13710 T13711 aux to,exclude
R3409 T13933 T13934 mark As,noted
R3410 T13711 T13704 xcomp exclude,appears
R3411 T13934 T13935 advcl noted,affect
R3412 T13712 T13713 det that,possibility
R3413 T13713 T13711 dobj possibility,exclude
R3414 T13936 T13935 punct ", ",affect
R3415 T13714 T13691 punct ", ",provide
R3416 T13937 T13938 mark whereas,die
R3417 T13938 T13935 advcl die,affect
R3418 T13715 T13691 cc and,provide
R3419 T13939 T13940 npadvmod Capn2,null
R3420 T13940 T13941 amod null,embryos
R3421 T13941 T13938 nsubj embryos,die
R3422 T13716 T13717 det no,activity
R3423 T13942 T13941 amod murine,embryos
R3424 T13943 T13944 amod prior,to
R3425 T13717 T13719 nsubjpass activity,observed
R3426 T13944 T13938 prep to,die
R3427 T13945 T13944 pobj implantation,to
R3428 T13946 T13935 punct ", ",affect
R3429 T13718 T13717 compound calpain,activity
R3430 T13947 T13948 amod homozygous,disruption
R3431 T13948 T13935 nsubj disruption,affect
R3432 T13949 T13948 prep of,disruption
R3433 T13719 T13691 conj observed,provide
R3434 T13950 T13951 det the,gene
R3435 T13951 T13949 pobj gene,of
R3436 T13720 T13719 auxpass was,observed
R3437 T13952 T13953 nmod μ,calpain
R3438 T13953 T13951 nmod calpain,gene
R3439 T13954 T13953 punct -,calpain
R3440 T13721 T13719 prep in,observed
R3441 T13955 T13951 amod large,gene
R3442 T13956 T13951 compound subunit,gene
R3443 T13722 T13723 poss our,hands
R3444 T13957 T13951 punct ", ",gene
R3445 T13958 T13951 appos Capn1,gene
R3446 T13959 T13935 punct ", ",affect
R3447 T13960 T13935 aux did,affect
R3448 T13961 T13935 neg not,affect
R3449 T13962 T13963 det the,viability
R3450 T14041 T14040 amod relative,amounts
R3451 T13963 T13935 dobj viability,affect
R3452 T13964 T13963 prep of,viability
R3453 T13965 T13964 pobj mice,of
R3454 T13966 T13967 punct [,33
R3455 T13967 T13935 parataxis 33,affect
R3456 T13968 T13967 punct ],33
R3457 T14042 T14013 punct .,are
R3458 T13969 T13935 punct .,affect
R3459 T13971 T13972 det The,phenotype
R3460 T14044 T14045 nsubj Platelets,contain
R3461 T13972 T13974 nsubj phenotype,involved
R3462 T13973 T13972 amod principal,phenotype
R3463 T14046 T14044 cc and,Platelets
R3464 T13975 T13972 acl observed,phenotype
R3465 T13976 T13975 prep as,observed
R3466 T13977 T13978 det a,result
R3467 T13978 T13976 pobj result,as
R3468 T14047 T14044 conj erythrocytes,Platelets
R3469 T13979 T13978 prep of,result
R3470 T13980 T13981 compound Capn1,deficiency
R3471 T13981 T13979 pobj deficiency,of
R3472 T14048 T14049 amod abundant,activity
R3473 T13982 T13983 det a,disturbance
R3474 T13983 T13974 dobj disturbance,involved
R3475 T13984 T13983 prep in,disturbance
R3476 T14049 T14045 dobj activity,contain
R3477 T13985 T13986 compound platelet,function
R3478 T13986 T13984 pobj function,in
R3479 T13987 T13983 punct ", ",disturbance
R3480 T14050 T14051 compound μ,calpain
R3481 T13988 T13989 advmod possibly,at
R3482 T13989 T13983 prep at,disturbance
R3483 T13990 T13991 det the,level
R3484 T14051 T14049 compound calpain,activity
R3485 T13991 T13989 pobj level,at
R3486 T13992 T13991 prep of,level
R3487 T13993 T13994 det the,phosphorylation
R3488 T14052 T14051 punct -,calpain
R3489 T13994 T13992 pobj phosphorylation,of
R3490 T13995 T13994 compound tyrosine,phosphorylation
R3491 T13996 T13994 prep of,phosphorylation
R3492 T14053 T14045 punct ", ",contain
R3493 T13997 T13998 amod certain,proteins
R3494 T13998 T13996 pobj proteins,of
R3495 T13999 T13998 acl involved,proteins
R3496 T14054 T14055 mark while,is
R3497 T14055 T14045 advcl is,contain
R3498 T14000 T13999 prep in,involved
R3499 T14001 T14002 compound platelet,activation
R3500 T14002 T14000 pobj activation,in
R3501 T14003 T13974 punct .,involved
R3502 T14056 T14057 compound m,calpain
R3503 T14005 T14006 preconj Both,activities
R3504 T14006 T14013 nsubj activities,are
R3505 T14057 T14059 compound calpain,activity
R3506 T14007 T14008 nmod μ,calpain
R3507 T14008 T14006 compound calpain,activities
R3508 T14009 T14007 punct -,μ
R3509 T14058 T14057 punct -,calpain
R3510 T14010 T14007 cc and,μ
R3511 T14011 T14007 conj m,μ
R3512 T14012 T14008 punct -,calpain
R3513 T14059 T14055 nsubj activity,is
R3514 T14014 T14013 acomp present,are
R3515 T14060 T14061 advmod barely,detectable
R3516 T14015 T14013 prep in,are
R3517 T14061 T14055 acomp detectable,is
R3518 T14062 T14045 punct .,contain
R3519 T14016 T14017 amod most,cells
R3520 T14017 T14015 pobj cells,in
R3521 T14018 T14017 amod mammalian,cells
R3522 T14064 T14065 nsubj Compensation,is
R3523 T14019 T14013 punct ", ",are
R3524 T14020 T14021 mark although,provide
R3525 T14066 T14064 prep for,Compensation
R3526 T14021 T14013 advcl provide,are
R3527 T14022 T14023 det the,data
R3528 T14023 T14021 nsubj data,provide
R3529 T14024 T14023 amod published,data
R3530 T14067 T14068 compound μ,calpain
R3531 T14025 T14023 punct ", ",data
R3532 T14026 T14023 prep owing,data
R3533 T14027 T14026 prep to,owing
R3534 T14068 T14070 compound calpain,deficiency
R3535 T14028 T14027 pobj weaknesses,to
R3536 T14029 T14028 prep in,weaknesses
R3537 T14030 T14031 det the,methodology
R3538 T14069 T14068 punct -,calpain
R3539 T14031 T14029 pobj methodology,in
R3540 T14032 T14031 amod available,methodology
R3541 T14033 T14021 punct ", ",provide
R3542 T14070 T14066 pobj deficiency,for
R3543 T14034 T14021 aux do,provide
R3544 T14035 T14021 neg not,provide
R3545 T14036 T14037 amod reliable,estimates
R3546 T14071 T14064 prep by,Compensation
R3547 T14037 T14021 dobj estimates,provide
R3548 T14038 T14037 prep of,estimates
R3549 T14039 T14040 poss their,amounts
R3550 T14072 T14073 compound m,calpain
R3551 T14040 T14038 pobj amounts,of
R3552 T14073 T14071 pobj calpain,by
R3553 T14074 T14073 punct -,calpain
R3554 T14075 T14065 advmod therefore,is
R3555 T14147 T14145 punct -,Capn2
R3556 T14076 T14077 advmod less,likely
R3557 T14148 T14145 punct /,Capn2
R3558 T14149 T14145 punct -,Capn2
R3559 T14077 T14065 acomp likely,is
R3560 T14150 T14151 mark that,is
R3561 T14151 T14140 ccomp is,demonstrates
R3562 T14078 T14077 prep in,likely
R3563 T14152 T14153 compound m,calpain
R3564 T14153 T14155 compound calpain,activity
R3565 T14154 T14153 punct -,calpain
R3566 T14079 T14078 pobj platelets,in
R3567 T14155 T14151 nsubj activity,is
R3568 T14156 T14151 acomp essential,is
R3569 T14157 T14156 prep for,essential
R3570 T14080 T14079 cc and,platelets
R3571 T14158 T14159 amod embryonic,development
R3572 T14159 T14157 pobj development,for
R3573 T14160 T14159 prep beyond,development
R3574 T14081 T14079 conj erythrocytes,platelets
R3575 T14161 T14162 det the,stage
R3576 T14162 T14160 pobj stage,beyond
R3577 T14082 T14077 prep than,likely
R3578 T14163 T14164 nummod 8,cell
R3579 T14164 T14162 compound cell,stage
R3580 T14165 T14164 punct -,cell
R3581 T14083 T14082 prep in,than
R3582 T14166 T14140 punct .,demonstrates
R3583 T14168 T14169 nsubj It,follows
R3584 T14084 T14085 amod other,types
R3585 T14170 T14171 mark that,are
R3586 T14171 T14169 ccomp are,follows
R3587 T14085 T14083 pobj types,in
R3588 T14172 T14173 advmod at,least
R3589 T14173 T14174 advmod least,functions
R3590 T14086 T14085 compound cell,types
R3591 T14174 T14171 nsubj functions,are
R3592 T14175 T14174 det some,functions
R3593 T14176 T14174 prep of,functions
R3594 T14087 T14065 punct .,is
R3595 T14177 T14178 compound μ,calpain
R3596 T14178 T14176 pobj calpain,of
R3597 T14179 T14178 punct -,calpain
R3598 T14089 T14090 prep As,is
R3599 T14180 T14178 cc and,calpain
R3600 T14181 T14182 compound m,calpain
R3601 T14182 T14178 conj calpain,calpain
R3602 T14183 T14182 punct -,calpain
R3603 T14184 T14171 acomp distinct,are
R3604 T14185 T14169 punct .,follows
R3605 T14091 T14092 det a,result
R3606 T14187 T14188 det The,cause
R3607 T14092 T14089 pobj result,As
R3608 T14188 T14190 nsubjpass cause,clarified
R3609 T14189 T14188 amod underlying,cause
R3610 T14093 T14090 punct ", ",is
R3611 T14191 T14188 prep of,cause
R3612 T14192 T14193 det the,lethality
R3613 T14193 T14191 pobj lethality,of
R3614 T14094 T14090 nsubj it,is
R3615 T14194 T14193 compound preimplantation,lethality
R3616 T14195 T14188 prep in,cause
R3617 T14196 T14197 nmod Capn2,embryos
R3618 T14095 T14090 neg not,is
R3619 T14197 T14195 pobj embryos,in
R3620 T14198 T14196 punct -,Capn2
R3621 T14096 T14090 acomp possible,is
R3622 T14199 T14196 punct /,Capn2
R3623 T14200 T14196 punct -,Capn2
R3624 T14201 T14190 aux has,clarified
R3625 T14097 T14098 aux to,determine
R3626 T14202 T14190 neg not,clarified
R3627 T14203 T14190 advmod yet,clarified
R3628 T14204 T14190 auxpass been,clarified
R3629 T14098 T14090 xcomp determine,is
R3630 T14205 T14190 punct .,clarified
R3631 T14099 T14100 mark whether,is
R3632 T14207 T14208 det The,embryos
R3633 T14208 T14212 nsubj embryos,present
R3634 T14209 T14208 nummod two,embryos
R3635 T14100 T14098 advcl is,determine
R3636 T14210 T14211 amod homozygous,null
R3637 T14211 T14208 amod null,embryos
R3638 T14101 T14102 det the,absence
R3639 T14213 T14208 acl identified,embryos
R3640 T14214 T14213 prep at,identified
R3641 T14215 T14216 det the,stage
R3642 T14102 T14100 nsubj absence,is
R3643 T14216 T14214 pobj stage,at
R3644 T14217 T14218 nummod 8,cell
R3645 T14218 T14216 compound cell,stage
R3646 T14103 T14102 prep of,absence
R3647 T14219 T14218 punct -,cell
R3648 T14220 T14212 aux did,present
R3649 T14221 T14212 neg not,present
R3650 T14104 T14105 amod marked,phenotype
R3651 T14222 T14223 det any,defects
R3652 T14223 T14212 dobj defects,present
R3653 T14105 T14103 pobj phenotype,of
R3654 T14224 T14223 amod obvious,defects
R3655 T14225 T14223 amod morphological,defects
R3656 T14226 T14212 punct .,present
R3657 T14228 T14229 advmod However,is
R3658 T14106 T14102 prep in,absence
R3659 T14230 T14229 punct ", ",is
R3660 T14231 T14232 det the,fact
R3661 T14232 T14229 nsubj fact,is
R3662 T14107 T14108 nmod Capn1,animals
R3663 T14233 T14234 mark that,proved
R3664 T14234 T14232 acl proved,fact
R3665 T14235 T14236 advmod only,embryos
R3666 T14108 T14106 pobj animals,in
R3667 T14236 T14234 nsubj embryos,proved
R3668 T14237 T14238 quantmod two,90
R3669 T14238 T14236 nummod 90,embryos
R3670 T14109 T14107 punct -,Capn1
R3671 T14239 T14238 quantmod out,90
R3672 T14240 T14238 quantmod of,90
R3673 T14241 T14242 advmod successfully,genotyped
R3674 T14110 T14107 punct /,Capn1
R3675 T14242 T14236 amod genotyped,embryos
R3676 T14243 T14236 amod pre-implantation,embryos
R3677 T14244 T14245 aux to,be
R3678 T14111 T14107 punct -,Capn1
R3679 T14245 T14234 xcomp be,proved
R3680 T14246 T14245 attr Capn2,be
R3681 T14247 T14246 punct -,Capn2
R3682 T14112 T14100 prep due,is
R3683 T14248 T14246 punct /,Capn2
R3684 T14249 T14246 punct -,Capn2
R3685 T14113 T14112 pcomp to,due
R3686 T14250 T14229 prep in,is
R3687 T14251 T14250 pobj itself,in
R3688 T14252 T14229 acomp revealing,is
R3689 T14114 T14115 det a,affect
R3690 T14115 T14112 pobj affect,due
R3691 T14116 T14115 amod compensatory,affect
R3692 T14253 T14229 punct .,is
R3693 T14117 T14115 prep by,affect
R3694 T14255 T14256 compound Preimplantation,lethality
R3695 T14256 T14257 nsubjpass lethality,attributed
R3696 T14118 T14119 det the,activity
R3697 T14258 T14256 acl resulting,lethality
R3698 T14259 T14258 prep from,resulting
R3699 T14119 T14117 pobj activity,by
R3700 T14260 T14261 compound gene,knockouts
R3701 T14261 T14259 pobj knockouts,from
R3702 T14120 T14119 amod remaining,activity
R3703 T14262 T14257 aux can,attributed
R3704 T14263 T14257 advmod typically,attributed
R3705 T14264 T14257 auxpass be,attributed
R3706 T14265 T14257 prep to,attributed
R3707 T14266 T14267 nummod two,causes
R3708 T14267 T14265 pobj causes,to
R3709 T14121 T14122 compound m,calpain
R3710 T14268 T14267 amod general,causes
R3711 T14269 T14257 punct .,attributed
R3712 T14122 T14119 compound calpain,activity
R3713 T14271 T14272 prep In,incurred
R3714 T14273 T14274 det some,cases
R3715 T14123 T14122 punct -,calpain
R3716 T14274 T14271 pobj cases,In
R3717 T14275 T14272 punct ", ",incurred
R3718 T14124 T14100 punct ", ",is
R3719 T14276 T14272 nsubjpass defects,incurred
R3720 T14277 T14272 auxpass are,incurred
R3721 T14278 T14272 prep in,incurred
R3722 T14125 T14100 cc or,is
R3723 T14126 T14127 mark whether,are
R3724 T14279 T14280 det the,program
R3725 T14127 T14100 conj are,is
R3726 T14280 T14278 pobj program,in
R3727 T14281 T14280 amod embryonic,program
R3728 T14282 T14280 compound differentiation,program
R3729 T14128 T14129 det the,functions
R3730 T14283 T14284 dep which,observed
R3731 T14284 T14280 relcl observed,program
R3732 T14285 T14284 aux can,observed
R3733 T14129 T14127 nsubj functions,are
R3734 T14286 T14284 advmod often,observed
R3735 T14287 T14284 auxpass be,observed
R3736 T14130 T14129 prep of,functions
R3737 T14288 T14284 advmod morphologically,observed
R3738 T14289 T14290 punct [,38
R3739 T14131 T14132 compound μ,calpain
R3740 T14290 T14284 parataxis 38,observed
R3741 T14291 T14290 punct ],38
R3742 T14292 T14272 punct .,incurred
R3743 T14132 T14130 pobj calpain,of
R3744 T14294 T14295 amod Null,embryos
R3745 T14295 T14296 nsubj embryos,survive
R3746 T14133 T14132 punct -,calpain
R3747 T14297 T14295 prep of,embryos
R3748 T14298 T14299 det this,type
R3749 T14134 T14127 advmod simply,are
R3750 T14299 T14297 pobj type,of
R3751 T14300 T14296 advmod often,survive
R3752 T14135 T14127 neg not,are
R3753 T14301 T14296 prep beyond,survive
R3754 T14302 T14303 det the,stage
R3755 T14303 T14301 pobj stage,beyond
R3756 T14136 T14127 acomp essential,are
R3757 T14304 T14303 compound morula,stage
R3758 T14305 T14296 punct ", ",survive
R3759 T14306 T14296 cc and,survive
R3760 T14307 T14308 det a,distribution
R3761 T14308 T14310 nsubjpass distribution,noted
R3762 T14137 T14090 punct .,is
R3763 T14309 T14308 amod Mendelian,distribution
R3764 T14310 T14296 conj noted,survive
R3765 T14311 T14308 prep of,distribution
R3766 T14139 T14140 prep In,demonstrates
R3767 T14312 T14313 amod embryonic,genotypes
R3768 T14313 T14311 pobj genotypes,of
R3769 T14314 T14310 auxpass is,noted
R3770 T14315 T14310 advmod usually,noted
R3771 T14141 T14139 pobj contrast,In
R3772 T14316 T14310 punct .,noted
R3773 T14318 T14319 prep In,thought
R3774 T14142 T14140 punct ", ",demonstrates
R3775 T14320 T14321 amod other,cases
R3776 T14321 T14318 pobj cases,In
R3777 T14322 T14319 punct ", ",thought
R3778 T14143 T14140 nsubj lethality,demonstrates
R3779 T14323 T14319 advmod however,thought
R3780 T14324 T14319 punct ", ",thought
R3781 T14144 T14143 prep in,lethality
R3782 T14325 T14326 det the,disruption
R3783 T14326 T14319 nsubjpass disruption,thought
R3784 T14327 T14326 compound gene,disruption
R3785 T14145 T14146 nmod Capn2,embryos
R3786 T14328 T14319 auxpass is,thought
R3787 T14329 T14330 aux to,compromise
R3788 T14146 T14144 pobj embryos,in
R3789 T14330 T14319 xcomp compromise,thought
R3790 T14331 T14330 advmod fatally,compromise
R3791 T14332 T14333 det the,viability
R3792 T14359 T14350 auxpass are,observed
R3793 T14333 T14330 dobj viability,compromise
R3794 T14334 T14333 prep of,viability
R3795 T14335 T14334 pobj cells,of
R3796 T14336 T14335 prep in,cells
R3797 T14360 T14350 advmod ever,observed
R3798 T14337 T14338 det the,embryo
R3799 T14338 T14336 pobj embryo,in
R3800 T14339 T14338 amod early,embryo
R3801 T14361 T14350 cc and,observed
R3802 T14340 T14341 punct [,39
R3803 T14341 T14330 parataxis 39,compromise
R3804 T14342 T14341 dep 3,39
R3805 T14362 T14363 amod homozygous,cells
R3806 T14343 T14341 punct ",",39
R3807 T14344 T14345 punct -,43
R3808 T14345 T14341 prep 43,39
R3809 T14363 T14366 nsubjpass cells,isolated
R3810 T14346 T14341 punct ],39
R3811 T14347 T14319 punct .,thought
R3812 T14349 T14350 prep In,observed
R3813 T14364 T14363 compound mutant,cells
R3814 T14351 T14352 det these,instances
R3815 T14352 T14349 pobj instances,In
R3816 T14353 T14350 punct ", ",observed
R3817 T14365 T14363 compound ES,cells
R3818 T14354 T14355 advmod only,embryos
R3819 T14355 T14350 nsubjpass embryos,observed
R3820 T14356 T14355 det a,embryos
R3821 T14366 T14350 conj isolated,observed
R3822 T14357 T14355 amod few,embryos
R3823 T14358 T14355 amod null,embryos
R3824 T14367 T14366 aux could,isolated
R3825 T14368 T14366 neg not,isolated
R3826 T14369 T14366 auxpass be,isolated
R3827 T14370 T14366 punct .,isolated
R3828 T14372 T14373 det The,knockout
R3829 T14465 T14467 compound calpain,involvement
R3830 T14373 T14375 nsubj knockout,fits
R3831 T14466 T14465 punct -,calpain
R3832 T14467 T14463 pobj involvement,of
R3833 T14468 T14467 prep in,involvement
R3834 T14374 T14373 compound Capn2,knockout
R3835 T14469 T14470 compound cell,proliferation
R3836 T14470 T14468 pobj proliferation,in
R3837 T14376 T14375 prep into,fits
R3838 T14471 T14467 prep in,involvement
R3839 T14472 T14473 amod certain,circumstances
R3840 T14473 T14471 pobj circumstances,in
R3841 T14474 T14462 punct ", ",reports
R3842 T14377 T14378 det the,category
R3843 T14475 T14462 prep including,reports
R3844 T14476 T14475 pobj reports,including
R3845 T14477 T14476 prep of,reports
R3846 T14378 T14376 pobj category,into
R3847 T14478 T14479 poss its,involvement
R3848 T14479 T14477 pobj involvement,of
R3849 T14480 T14479 prep in,involvement
R3850 T14379 T14378 amod latter,category
R3851 T14481 T14482 compound chromosome,segregation
R3852 T14482 T14480 pobj segregation,in
R3853 T14380 T14375 punct .,fits
R3854 T14483 T14482 prep during,segregation
R3855 T14484 T14483 pobj mitosis,during
R3856 T14485 T14486 punct [,44
R3857 T14382 T14383 mark If,is
R3858 T14486 T14484 parataxis 44,mitosis
R3859 T14487 T14486 punct ],44
R3860 T14488 T14489 advmod as,as
R3861 T14489 T14483 cc as,during
R3862 T14490 T14489 advmod well,as
R3863 T14491 T14483 conj during,during
R3864 T14492 T14491 pobj meiosis,during
R3865 T14383 T14385 advcl is,is
R3866 T14493 T14494 punct [,45
R3867 T14494 T14492 parataxis 45,meiosis
R3868 T14495 T14494 punct ],45
R3869 T14384 T14383 nsubj it,is
R3870 T14496 T14459 punct .,been
R3871 T14386 T14383 acomp true,is
R3872 T14498 T14499 nsubj Defects,contribute
R3873 T14500 T14498 prep in,Defects
R3874 T14501 T14500 pobj migration,in
R3875 T14387 T14388 mark that,is
R3876 T14502 T14498 punct ", ",Defects
R3877 T14503 T14498 acl reported,Defects
R3878 T14504 T14503 prep in,reported
R3879 T14388 T14383 ccomp is,is
R3880 T14505 T14506 nmod Capn4,cells
R3881 T14506 T14504 pobj cells,in
R3882 T14507 T14505 punct -,Capn4
R3883 T14389 T14390 compound m,calpain
R3884 T14508 T14505 punct /,Capn4
R3885 T14509 T14505 punct -,Capn4
R3886 T14390 T14388 nsubj calpain,is
R3887 T14510 T14511 punct [,9
R3888 T14511 T14503 parataxis 9,reported
R3889 T14512 T14511 punct ],9
R3890 T14391 T14390 punct -,calpain
R3891 T14513 T14499 punct ", ",contribute
R3892 T14514 T14499 aux could,contribute
R3893 T14515 T14499 prep to,contribute
R3894 T14392 T14388 acomp essential,is
R3895 T14516 T14517 amod failed,development
R3896 T14393 T14392 prep for,essential
R3897 T14517 T14515 pobj development,to
R3898 T14518 T14517 amod embryonic,development
R3899 T14519 T14499 punct .,contribute
R3900 T14394 T14395 det some,aspect
R3901 T14521 T14522 det The,association
R3902 T14522 T14523 nsubjpass association,noted
R3903 T14395 T14393 pobj aspect,for
R3904 T14524 T14522 prep between,association
R3905 T14396 T14395 prep of,aspect
R3906 T14525 T14524 pobj calpain,between
R3907 T14526 T14525 cc and,calpain
R3908 T14397 T14398 compound cell,viability
R3909 T14527 T14528 compound cell,viability
R3910 T14528 T14525 conj viability,calpain
R3911 T14529 T14523 aux has,noted
R3912 T14398 T14396 pobj viability,of
R3913 T14530 T14523 auxpass been,noted
R3914 T14531 T14523 prep in,noted
R3915 T14532 T14533 nmod Capn4,MEFs
R3916 T14533 T14531 pobj MEFs,in
R3917 T14534 T14532 punct -,Capn4
R3918 T14535 T14532 punct /,Capn4
R3919 T14536 T14532 punct -,Capn4
R3920 T14399 T14385 punct ", ",is
R3921 T14537 T14533 cc and,MEFs
R3922 T14538 T14539 amod other,lines
R3923 T14400 T14401 det the,survival
R3924 T14401 T14385 nsubj survival,is
R3925 T14539 T14533 conj lines,MEFs
R3926 T14540 T14539 compound cell,lines
R3927 T14541 T14523 punct ", ",noted
R3928 T14402 T14401 prep of,survival
R3929 T14542 T14543 mark although,distinguish
R3930 T14543 T14523 advcl distinguish,noted
R3931 T14403 T14404 det a,embryos
R3932 T14544 T14545 det the,work
R3933 T14545 T14543 nsubj work,distinguish
R3934 T14404 T14402 pobj embryos,of
R3935 T14546 T14545 amod reported,work
R3936 T14547 T14543 advmod frequently,distinguish
R3937 T14405 T14404 amod few,embryos
R3938 T14548 T14543 aux did,distinguish
R3939 T14549 T14543 neg not,distinguish
R3940 T14550 T14543 prep between,distinguish
R3941 T14406 T14404 nmod Capn2,embryos
R3942 T14551 T14552 nmod μ,calpain
R3943 T14552 T14550 pobj calpain,between
R3944 T14407 T14406 punct -,Capn2
R3945 T14553 T14551 punct -,μ
R3946 T14554 T14551 cc and,μ
R3947 T14408 T14406 punct /,Capn2
R3948 T14555 T14551 conj m,μ
R3949 T14556 T14552 punct -,calpain
R3950 T14557 T14543 punct ", ",distinguish
R3951 T14409 T14406 punct -,Capn2
R3952 T14558 T14543 cc and,distinguish
R3953 T14559 T14560 aux did,show
R3954 T14410 T14401 prep to,survival
R3955 T14560 T14543 conj show,distinguish
R3956 T14561 T14560 neg not,show
R3957 T14562 T14563 mark that,was
R3958 T14411 T14412 det the,stage
R3959 T14563 T14560 ccomp was,show
R3960 T14564 T14563 nsubj calpain,was
R3961 T14565 T14566 advmod strictly,essential
R3962 T14412 T14410 pobj stage,to
R3963 T14566 T14563 acomp essential,was
R3964 T14567 T14568 punct [,6
R3965 T14568 T14560 parataxis 6,show
R3966 T14569 T14568 nummod 5,6
R3967 T14413 T14414 nummod 8,cell
R3968 T14414 T14412 compound cell,stage
R3969 T14570 T14568 punct ",",6
R3970 T14571 T14568 punct ],6
R3971 T14572 T14523 punct .,noted
R3972 T14415 T14414 punct -,cell
R3973 T14574 T14575 prep In,shown
R3974 T14416 T14417 advmod most,likely
R3975 T14576 T14577 preconj both,cells
R3976 T14577 T14574 pobj cells,In
R3977 T14417 T14418 advmod likely,due
R3978 T14578 T14577 compound CHO,cells
R3979 T14579 T14577 cc and,cells
R3980 T14580 T14581 nmod Capn4,MEFs
R3981 T14418 T14385 prep due,is
R3982 T14581 T14577 conj MEFs,cells
R3983 T14582 T14580 punct -,Capn4
R3984 T14583 T14580 punct /,Capn4
R3985 T14419 T14418 pcomp to,due
R3986 T14584 T14580 punct -,Capn4
R3987 T14585 T14575 punct ", ",shown
R3988 T14420 T14421 det the,persistence
R3989 T14586 T14575 nsubjpass calpain,shown
R3990 T14587 T14575 auxpass was,shown
R3991 T14588 T14589 aux to,influence
R3992 T14421 T14418 pobj persistence,due
R3993 T14589 T14575 xcomp influence,shown
R3994 T14590 T14591 compound cell,proliferation
R3995 T14591 T14589 dobj proliferation,influence
R3996 T14422 T14421 prep of,persistence
R3997 T14592 T14589 punct ", ",influence
R3998 T14593 T14594 cc but,at
R3999 T14594 T14589 prep at,influence
R4000 T14423 T14424 det some,calpain
R4001 T14595 T14594 advmod only,at
R4002 T14596 T14597 advmod very,low
R4003 T14597 T14598 amod low,densities
R4004 T14424 T14422 pobj calpain,of
R4005 T14598 T14594 pobj densities,at
R4006 T14599 T14598 compound cell,densities
R4007 T14600 T14601 punct [,5
R4008 T14425 T14424 amod maternal,calpain
R4009 T14601 T14589 parataxis 5,influence
R4010 T14602 T14601 punct ],5
R4011 T14426 T14424 compound m,calpain
R4012 T14603 T14575 punct .,shown
R4013 T14605 T14606 nsubjpass Calpain,associated
R4014 T14427 T14424 punct -,calpain
R4015 T14607 T14606 aux has,associated
R4016 T14428 T14424 appos mRNA,calpain
R4017 T14608 T14606 advmod also,associated
R4018 T14609 T14606 auxpass been,associated
R4019 T14610 T14606 prep with,associated
R4020 T14611 T14610 pobj progression,with
R4021 T14612 T14611 prep through,progression
R4022 T14429 T14428 cc and,mRNA
R4023 T14613 T14614 det the,stage
R4024 T14614 T14612 pobj stage,through
R4025 T14615 T14614 compound G1,stage
R4026 T14430 T14429 punct /,and
R4027 T14616 T14614 prep of,stage
R4028 T14617 T14618 det the,cycle
R4029 T14431 T14429 cc or,and
R4030 T14618 T14616 pobj cycle,of
R4031 T14619 T14618 compound cell,cycle
R4032 T14620 T14621 punct [,6
R4033 T14432 T14428 conj protein,mRNA
R4034 T14621 T14606 parataxis 6,associated
R4035 T14622 T14621 punct ],6
R4036 T14623 T14606 punct .,associated
R4037 T14433 T14421 prep through,persistence
R4038 T14434 T14435 quantmod 2,3
R4039 T14625 T14626 advmod Furthermore,reputed
R4040 T14435 T14437 nummod 3,divisions
R4041 T14627 T14626 punct ", ",reputed
R4042 T14436 T14435 punct –,3
R4043 T14628 T14626 nsubjpass some,reputed
R4044 T14629 T14628 prep of,some
R4045 T14630 T14631 det the,proteins
R4046 T14437 T14433 pobj divisions,through
R4047 T14631 T14629 pobj proteins,of
R4048 T14632 T14631 acl known,proteins
R4049 T14438 T14437 compound cell,divisions
R4050 T14633 T14634 aux to,involved
R4051 T14634 T14632 xcomp involved,known
R4052 T14635 T14634 auxpass be,involved
R4053 T14439 T14385 punct .,is
R4054 T14636 T14634 prep in,involved
R4055 T14637 T14638 compound cell,cycle
R4056 T14638 T14639 compound cycle,progression
R4057 T14441 T14442 det The,reasons
R4058 T14639 T14636 pobj progression,in
R4059 T14640 T14631 punct ", ",proteins
R4060 T14641 T14642 amod such,as
R4061 T14642 T14631 prep as,proteins
R4062 T14442 T14444 nsubj reasons,are
R4063 T14643 T14642 pobj p53,as
R4064 T14644 T14643 punct ", ",p53
R4065 T14645 T14643 conj p107,p53
R4066 T14443 T14442 amod mechanistic,reasons
R4067 T14646 T14645 punct ", ",p107
R4068 T14647 T14648 compound cyclin,D1
R4069 T14445 T14442 prep for,reasons
R4070 T14648 T14645 conj D1,p107
R4071 T14649 T14648 punct ", ",D1
R4072 T14650 T14648 cc and,D1
R4073 T14651 T14648 conj p27kip1,D1
R4074 T14652 T14626 auxpass are,reputed
R4075 T14653 T14654 aux to,be
R4076 T14446 T14445 pobj lethality,for
R4077 T14654 T14626 xcomp be,reputed
R4078 T14655 T14656 compound calpain,substrates
R4079 T14656 T14654 attr substrates,be
R4080 T14447 T14442 prep in,reasons
R4081 T14657 T14658 punct [,3
R4082 T14658 T14654 parataxis 3,be
R4083 T14659 T14658 punct ],3
R4084 T14448 T14449 det the,absence
R4085 T14660 T14626 punct .,reputed
R4086 T14662 T14663 compound Sperm,binding
R4087 T14449 T14447 pobj absence,in
R4088 T14663 T14664 nsubj binding,leads
R4089 T14450 T14449 prep of,absence
R4090 T14665 T14663 prep to,binding
R4091 T14666 T14667 det the,oocyte
R4092 T14667 T14665 pobj oocyte,to
R4093 T14451 T14452 compound m,calpain
R4094 T14668 T14664 prep to,leads
R4095 T14669 T14670 amod increased,calcium
R4096 T14670 T14668 pobj calcium,to
R4097 T14452 T14450 pobj calpain,of
R4098 T14671 T14670 amod cytoplasmic,calcium
R4099 T14672 T14673 dep which,triggers
R4100 T14673 T14670 relcl triggers,calcium
R4101 T14453 T14452 punct -,calpain
R4102 T14674 T14675 det the,reaction
R4103 T14675 T14673 dobj reaction,triggers
R4104 T14454 T14444 advmod still,are
R4105 T14455 T14444 acomp unclear,are
R4106 T14676 T14675 compound acrosome,reaction
R4107 T14456 T14444 punct .,are
R4108 T14677 T14678 punct [,46
R4109 T14678 T14664 parataxis 46,leads
R4110 T14679 T14678 punct ],46
R4111 T14458 T14459 expl There,been
R4112 T14680 T14664 punct .,leads
R4113 T14682 T14683 preconj Both,μ
R4114 T14683 T14684 nmod μ,calpain
R4115 T14460 T14459 aux have,been
R4116 T14684 T14689 nsubjpass calpain,detected
R4117 T14685 T14683 punct -,μ
R4118 T14686 T14683 cc and,μ
R4119 T14687 T14683 conj m,μ
R4120 T14461 T14462 amod several,reports
R4121 T14688 T14684 punct -,calpain
R4122 T14689 T14693 ccomp detected,implicated
R4123 T14690 T14689 aux have,detected
R4124 T14691 T14689 advmod recently,detected
R4125 T14692 T14689 auxpass been,detected
R4126 T14462 T14459 attr reports,been
R4127 T14694 T14689 prep in,detected
R4128 T14695 T14696 compound rodent,sperm
R4129 T14696 T14694 pobj sperm,in
R4130 T14463 T14462 prep of,reports
R4131 T14697 T14698 punct [,47
R4132 T14698 T14696 parataxis 47,sperm
R4133 T14699 T14698 punct ],47
R4134 T14464 T14465 compound m,calpain
R4135 T14700 T14696 cc and,sperm
R4136 T14701 T14696 conj oocytes,sperm
R4137 T14702 T14703 punct [,45
R4138 T14781 T14782 amod defective,segregation
R4139 T14703 T14701 parataxis 45,oocytes
R4140 T14704 T14703 punct ],45
R4141 T14705 T14689 punct .,detected
R4142 T14706 T14707 compound m,calpain
R4143 T14782 T14778 conj segregation,Polyspermy
R4144 T14707 T14693 nsubjpass calpain,implicated
R4145 T14708 T14707 punct -,calpain
R4146 T14709 T14693 auxpass was,implicated
R4147 T14783 T14782 compound chromosome,segregation
R4148 T14784 T14779 aux would,have
R4149 T14710 T14693 prep in,implicated
R4150 T14711 T14712 det the,reaction
R4151 T14712 T14710 pobj reaction,in
R4152 T14785 T14779 advmod both,have
R4153 T14713 T14712 compound acrosome,reaction
R4154 T14714 T14715 punct [,47
R4155 T14786 T14787 amod lethal,effects
R4156 T14715 T14712 parataxis 47,reaction
R4157 T14716 T14715 punct ],47
R4158 T14717 T14718 dep which,correlated
R4159 T14718 T14712 relcl correlated,reaction
R4160 T14787 T14779 dobj effects,have
R4161 T14719 T14718 prep with,correlated
R4162 T14720 T14721 det a,translocation
R4163 T14788 T14787 prep on,effects
R4164 T14721 T14719 pobj translocation,with
R4165 T14722 T14721 prep of,translocation
R4166 T14789 T14790 amod early,development
R4167 T14723 T14724 compound m,calpain
R4168 T14724 T14722 pobj calpain,of
R4169 T14725 T14724 punct -,calpain
R4170 T14790 T14788 pobj development,on
R4171 T14726 T14721 prep to,translocation
R4172 T14727 T14728 det the,membrane
R4173 T14728 T14726 pobj membrane,to
R4174 T14729 T14728 amod cortical,membrane
R4175 T14730 T14728 prep in,membrane
R4176 T14731 T14730 pobj oocytes,in
R4177 T14791 T14790 amod embryonic,development
R4178 T14732 T14733 advmod where,participate
R4179 T14733 T14728 relcl participate,membrane
R4180 T14734 T14733 nsubj it,participate
R4181 T14792 T14779 punct .,have
R4182 T14735 T14733 aux might,participate
R4183 T14736 T14733 prep in,participate
R4184 T14737 T14738 det the,release
R4185 T14794 T14795 nsubjpass It,stressed
R4186 T14738 T14736 pobj release,in
R4187 T14739 T14738 prep of,release
R4188 T14740 T14741 amod cortical,contents
R4189 T14741 T14739 pobj contents,of
R4190 T14796 T14795 aux should,stressed
R4191 T14742 T14741 compound granule,contents
R4192 T14743 T14741 acl required,contents
R4193 T14744 T14745 aux to,prevent
R4194 T14745 T14743 advcl prevent,required
R4195 T14797 T14795 advmod also,stressed
R4196 T14746 T14745 dobj polyspermy,prevent
R4197 T14747 T14748 punct [,45
R4198 T14748 T14745 parataxis 45,prevent
R4199 T14749 T14748 punct ],45
R4200 T14750 T14693 punct .,implicated
R4201 T14798 T14795 auxpass be,stressed
R4202 T14752 T14753 compound m,calpain
R4203 T14753 T14755 nsubj calpain,relocalized
R4204 T14754 T14753 punct -,calpain
R4205 T14799 T14800 mark that,maintained
R4206 T14756 T14755 advmod also,relocalized
R4207 T14800 T14795 ccomp maintained,stressed
R4208 T14757 T14755 prep to,relocalized
R4209 T14758 T14759 det the,spindle
R4210 T14759 T14757 pobj spindle,to
R4211 T14801 T14802 nmod Capn4,cells
R4212 T14760 T14759 nmod oocyte,spindle
R4213 T14761 T14759 amod meiotic,spindle
R4214 T14762 T14755 prep after,relocalized
R4215 T14802 T14800 nsubjpass cells,maintained
R4216 T14763 T14762 pobj fertilization,after
R4217 T14764 T14755 punct ", ",relocalized
R4218 T14803 T14801 punct -,Capn4
R4219 T14765 T14766 dep were,involved
R4220 T14766 T14755 ccomp involved,relocalized
R4221 T14767 T14766 nsubjpass it,involved
R4222 T14804 T14801 punct /,Capn4
R4223 T14768 T14766 aux could,involved
R4224 T14769 T14766 auxpass be,involved
R4225 T14770 T14766 prep in,involved
R4226 T14771 T14772 compound chromosome,segregation
R4227 T14772 T14770 pobj segregation,in
R4228 T14773 T14774 punct [,45
R4229 T14805 T14801 punct -,Capn4
R4230 T14774 T14766 parataxis 45,involved
R4231 T14775 T14774 punct ],45
R4232 T14776 T14755 punct .,relocalized
R4233 T14806 T14802 nmod ES,cells
R4234 T14778 T14779 nsubj Polyspermy,have
R4235 T14807 T14806 cc and,ES
R4236 T14780 T14778 cc or,Polyspermy
R4237 T14808 T14806 conj MEF,ES
R4238 T14809 T14802 prep from,cells
R4239 T14810 T14811 det the,allele
R4240 T14811 T14809 pobj allele,from
R4241 T14812 T14811 amod presumptive,allele
R4242 T14888 T14887 punct ", ",exist
R4243 T14813 T14811 amod hypomorphic,allele
R4244 T14889 T14890 npadvmod calpain,independent
R4245 T14814 T14800 aux can,maintained
R4246 T14890 T14892 amod independent,mechanisms
R4247 T14891 T14890 punct -,independent
R4248 T14892 T14887 nsubj mechanisms,exist
R4249 T14815 T14800 auxpass be,maintained
R4250 T14893 T14892 prep for,mechanisms
R4251 T14894 T14893 pcomp protecting,for
R4252 T14816 T14800 prep in,maintained
R4253 T14895 T14896 compound cell,viability
R4254 T14896 T14894 dobj viability,protecting
R4255 T14817 T14816 pobj culture,in
R4256 T14897 T14887 aux might,exist
R4257 T14898 T14887 prep in,exist
R4258 T14899 T14900 det these,lines
R4259 T14818 T14800 prep despite,maintained
R4260 T14900 T14898 pobj lines,in
R4261 T14901 T14900 compound cell,lines
R4262 T14819 T14820 det an,lack
R4263 T14902 T14903 dep that,are
R4264 T14903 T14887 ccomp are,exist
R4265 T14904 T14903 acomp absent,are
R4266 T14820 T14818 pobj lack,despite
R4267 T14905 T14903 prep in,are
R4268 T14906 T14907 amod early,cells
R4269 T14821 T14820 amod apparent,lack
R4270 T14907 T14905 pobj cells,in
R4271 T14908 T14907 amod embryonic,cells
R4272 T14909 T14887 punct .,exist
R4273 T14822 T14820 prep of,lack
R4274 T14911 T14912 det The,failure
R4275 T14912 T14914 nsubj failure,suggests
R4276 T14823 T14824 compound calpain,activity
R4277 T14913 T14912 amod repeated,failure
R4278 T14915 T14916 aux to,achieve
R4279 T14916 T14912 acl achieve,failure
R4280 T14917 T14918 compound gene,conversion
R4281 T14824 T14822 pobj activity,of
R4282 T14918 T14916 dobj conversion,achieve
R4283 T14919 T14918 prep of,conversion
R4284 T14920 T14919 pobj Capn2,of
R4285 T14825 T14800 punct ", ",maintained
R4286 T14921 T14920 punct +,Capn2
R4287 T14922 T14920 punct /,Capn2
R4288 T14923 T14920 punct -,Capn2
R4289 T14826 T14827 mark as,assessed
R4290 T14924 T14918 prep to,conversion
R4291 T14925 T14924 pobj Capn2,to
R4292 T14827 T14800 advcl assessed,maintained
R4293 T14926 T14925 punct -,Capn2
R4294 T14927 T14925 punct /,Capn2
R4295 T14928 T14925 punct -,Capn2
R4296 T14828 T14827 prep by,assessed
R4297 T14929 T14930 compound ES,cells
R4298 T14930 T14918 appos cells,conversion
R4299 T14829 T14830 compound casein,zymography
R4300 T14931 T14918 prep by,conversion
R4301 T14932 T14931 pobj selection,by
R4302 T14933 T14932 prep of,selection
R4303 T14830 T14828 pobj zymography,by
R4304 T14934 T14933 pobj clones,of
R4305 T14935 T14932 prep in,selection
R4306 T14831 T14828 cc or,by
R4307 T14936 T14937 amod high,concentrations
R4308 T14937 T14935 pobj concentrations,in
R4309 T14938 T14937 prep of,concentrations
R4310 T14832 T14828 conj by,by
R4311 T14939 T14938 pobj G418,of
R4312 T14940 T14941 mark that,compromises
R4313 T14833 T14834 det the,appearance
R4314 T14941 T14914 ccomp compromises,suggests
R4315 T14942 T14943 det the,state
R4316 T14943 T14941 nsubj state,compromises
R4317 T14834 T14832 pobj appearance,by
R4318 T14944 T14943 amod homozygous,state
R4319 T14945 T14943 compound mutant,state
R4320 T14946 T14941 advmod somehow,compromises
R4321 T14835 T14834 prep of,appearance
R4322 T14947 T14948 compound cell,viability
R4323 T14948 T14941 dobj viability,compromises
R4324 T14836 T14837 amod characteristic,products
R4325 T14949 T14948 cc or,viability
R4326 T14950 T14951 amod clonal,selection
R4327 T14951 T14948 conj selection,viability
R4328 T14837 T14835 pobj products,of
R4329 T14952 T14951 prep of,selection
R4330 T14953 T14954 compound ES,cells
R4331 T14954 T14952 pobj cells,of
R4332 T14955 T14914 punct .,suggests
R4333 T14838 T14837 compound spectrin,products
R4334 T14957 T14958 nsubj Efforts,are
R4335 T14959 T14960 advmod under,way
R4336 T14839 T14837 compound breakdown,products
R4337 T14960 T14958 advmod way,are
R4338 T14961 T14962 aux to,express
R4339 T14840 T14795 punct .,stressed
R4340 T14962 T14958 xcomp express,are
R4341 T14963 T14964 det the,subunit
R4342 T14842 T14843 nsubj It,is
R4343 T14844 T14843 acomp conceivable,is
R4344 T14964 T14962 dobj subunit,express
R4345 T14965 T14964 nmod m,subunit
R4346 T14966 T14965 punct -,m
R4347 T14845 T14843 punct ", ",is
R4348 T14967 T14965 nummod 80,m
R4349 T14968 T14964 compound k,subunit
R4350 T14969 T14964 prep from,subunit
R4351 T14846 T14847 mark as,discussed
R4352 T14970 T14971 det a,transgene
R4353 T14971 T14969 pobj transgene,from
R4354 T14847 T14843 advcl discussed,is
R4355 T14972 T14971 compound Capn2,transgene
R4356 T14973 T14971 compound cDNA,transgene
R4357 T14848 T14847 advmod earlier,discussed
R4358 T14974 T14971 compound rescue,transgene
R4359 T14975 T14976 amod prior,to
R4360 T14976 T14962 prep to,express
R4361 T14849 T14843 punct ", ",is
R4362 T14977 T14978 compound gene,conversion
R4363 T14978 T14976 pobj conversion,to
R4364 T14979 T14962 prep in,express
R4365 T14850 T14851 mark that,retained
R4366 T14980 T14979 pobj order,in
R4367 T14981 T14982 aux to,rescue
R4368 T14851 T14843 ccomp retained,is
R4369 T14982 T14980 acl rescue,order
R4370 T14983 T14982 advmod preemptively,rescue
R4371 T14984 T14985 compound m,calpain
R4372 T14852 T14853 det a,amount
R4373 T14985 T14987 compound calpain,activity
R4374 T14986 T14985 punct -,calpain
R4375 T14987 T14982 dobj activity,rescue
R4376 T14853 T14851 nsubjpass amount,retained
R4377 T14988 T14989 mark before,lost
R4378 T14989 T14982 advcl lost,rescue
R4379 T14990 T14991 det the,allele
R4380 T14854 T14853 compound trace,amount
R4381 T14991 T14989 nsubjpass allele,lost
R4382 T14992 T14991 amod remaining,allele
R4383 T14993 T14991 amod endogenous,allele
R4384 T14855 T14853 prep of,amount
R4385 T14856 T14857 compound calpain,activity
R4386 T14994 T14995 amod wild,type
R4387 T14995 T14991 compound type,allele
R4388 T14996 T14995 punct -,type
R4389 T14857 T14855 pobj activity,of
R4390 T14997 T14989 auxpass is,lost
R4391 T14998 T14958 punct .,are
R4392 T14858 T14853 punct ", ",amount
R4393 T15000 T15001 advmod However,hampered
R4394 T14859 T14853 prep beneath,amount
R4395 T15002 T15001 punct ", ",hampered
R4396 T15003 T15001 nsubj difficulty,hampered
R4397 T14860 T14859 pobj levels,beneath
R4398 T15004 T15003 prep in,difficulty
R4399 T15005 T15004 pcomp achieving,in
R4400 T15006 T15007 amod stable,expression
R4401 T14861 T14860 amod detectable,levels
R4402 T15007 T15005 dobj expression,achieving
R4403 T15008 T15007 prep of,expression
R4404 T15009 T15010 det the,transgene
R4405 T14862 T14861 prep by,detectable
R4406 T15010 T15008 pobj transgene,of
R4407 T15011 T15010 compound rescue,transgene
R4408 T14863 T14864 det these,methods
R4409 T15012 T15001 aux has,hampered
R4410 T15013 T15014 advmod thus,far
R4411 T15014 T15001 advmod far,hampered
R4412 T14864 T14862 pobj methods,by
R4413 T15015 T15016 det these,attempts
R4414 T15016 T15001 dobj attempts,hampered
R4415 T14865 T14851 punct ", ",retained
R4416 T15017 T15001 punct .,hampered
R4417 T14866 T14851 auxpass is,retained
R4418 T15019 T15020 nummod One,curiosity
R4419 T15020 T15021 nsubj curiosity,was
R4420 T14867 T14851 prep from,retained
R4421 T15022 T15023 dep which,arose
R4422 T15023 T15020 relcl arose,curiosity
R4423 T14868 T14869 det this,allele
R4424 T15024 T15023 prep during,arose
R4425 T15025 T15026 det the,genotyping
R4426 T15026 T15024 pobj genotyping,during
R4427 T14869 T14867 pobj allele,from
R4428 T15027 T15026 prep of,genotyping
R4429 T15028 T15027 pobj progeny,of
R4430 T15029 T15028 prep from,progeny
R4431 T14870 T14869 compound mutant,allele
R4432 T15030 T15031 amod heterozygous,interbreeding
R4433 T15031 T15029 pobj interbreeding,from
R4434 T15032 T15033 det the,ratio
R4435 T15033 T15021 attr ratio,was
R4436 T15034 T15035 advmod highly,non-Mendelian
R4437 T14871 T14869 compound Capn4,allele
R4438 T15035 T15033 amod non-Mendelian,ratio
R4439 T15036 T15033 prep of,ratio
R4440 T15037 T15036 pobj Capn2,of
R4441 T14872 T14843 punct ", ",is
R4442 T15038 T15037 punct +,Capn2
R4443 T15039 T15037 punct /,Capn2
R4444 T15040 T15037 punct +,Capn2
R4445 T14873 T14843 cc and,is
R4446 T15041 T15042 punct (,%
R4447 T15042 T15037 parataxis %,Capn2
R4448 T15043 T15042 nummod 11.6,%
R4449 T14874 T14875 nsubj it,is
R4450 T15044 T15042 punct ),%
R4451 T15045 T15033 prep to,ratio
R4452 T14875 T14843 conj is,is
R4453 T15046 T15045 pobj Capn2,to
R4454 T15047 T15046 punct +,Capn2
R4455 T15048 T15046 punct /,Capn2
R4456 T14876 T14875 acomp sufficient,is
R4457 T15049 T15046 punct -,Capn2
R4458 T14877 T14876 prep for,sufficient
R4459 T15050 T15051 punct (,%
R4460 T14878 T14877 pcomp maintaining,for
R4461 T15051 T15046 parataxis %,Capn2
R4462 T15052 T15051 nummod 88.4,%
R4463 T14879 T14880 det the,viability
R4464 T15053 T15051 punct ),%
R4465 T15054 T15033 prep of,ratio
R4466 T15055 T15054 pobj weanlings,of
R4467 T14880 T14878 dobj viability,maintaining
R4468 T15056 T15021 punct .,was
R4469 T15058 T15059 prep At,is
R4470 T14881 T14880 prep of,viability
R4471 T15060 T15058 amod present,At
R4472 T14882 T14883 det the,cells
R4473 T15061 T15059 punct ", ",is
R4474 T15062 T15059 expl there,is
R4475 T14883 T14881 pobj cells,of
R4476 T15063 T15064 det no,explanation
R4477 T15064 T15059 attr explanation,is
R4478 T15065 T15064 amod obvious,explanation
R4479 T14884 T14875 punct .,is
R4480 T15066 T15064 prep for,explanation
R4481 T15067 T15068 det this,result
R4482 T14886 T14887 advmod Furthermore,exist
R4483 T15068 T15066 pobj result,for
R4484 T15069 T15059 punct .,is
R4485 T15071 T15072 nsubj Crosses,produced
R4486 T15073 T15071 prep between,Crosses
R4487 T15074 T15075 amod wild,type
R4488 T15100 T15098 pobj line,involving
R4489 T15075 T15077 nmod type,mice
R4490 T15076 T15075 punct -,type
R4491 T15077 T15073 pobj mice,between
R4492 T15078 T15075 cc and,type
R4493 T15079 T15075 conj heterozygous,type
R4494 T15080 T15072 advmod also,produced
R4495 T15101 T15100 nmod Capn4,line
R4496 T15081 T15072 dobj progeny,produced
R4497 T15082 T15081 prep with,progeny
R4498 T15083 T15084 det a,proportion
R4499 T15084 T15082 pobj proportion,with
R4500 T15102 T15100 amod transgenic,line
R4501 T15085 T15084 amod greater,proportion
R4502 T15103 T15100 acl generated,line
R4503 T15086 T15087 mark than,expected
R4504 T15087 T15085 advcl expected,greater
R4505 T15104 T15103 agent by,generated
R4506 T15088 T15084 prep of,proportion
R4507 T15089 T15090 amod heterozygous,offspring
R4508 T15105 T15104 pobj Zimmerman,by
R4509 T15090 T15088 pobj offspring,of
R4510 T15091 T15072 punct .,produced
R4511 T15106 T15105 cc and,Zimmerman
R4512 T15093 T15094 advmod Interestingly,yielded
R4513 T15107 T15105 conj colleagues,Zimmerman
R4514 T15095 T15094 punct ", ",yielded
R4515 T15096 T15097 amod heterozygous,crosses
R4516 T15097 T15094 nsubj crosses,yielded
R4517 T15108 T15094 dobj progeny,yielded
R4518 T15098 T15097 prep involving,crosses
R4519 T15099 T15100 det the,line
R4520 T15109 T15108 prep with,progeny
R4521 T15110 T15111 det a,skewing
R4522 T15111 T15109 pobj skewing,with
R4523 T15112 T15111 amod similar,skewing
R4524 T15113 T15114 punct ", ",extreme
R4525 T15114 T15111 parataxis extreme,skewing
R4526 T15115 T15114 mark if,extreme
R4527 T15116 T15114 advmod less,extreme
R4528 T15117 T15114 punct ", ",extreme
R4529 T15118 T15111 prep in,skewing
R4530 T15119 T15118 pobj favor,in
R4531 T15120 T15119 prep of,favor
R4532 T15121 T15122 det the,genotype
R4533 T15122 T15120 pobj genotype,of
R4534 T15123 T15122 amod heterozygous,genotype
R4535 T15124 T15094 punct .,yielded
R4536 T15126 T15127 prep Out,were
R4537 T15128 T15126 prep of,Out
R4538 T15129 T15130 det a,total
R4539 T15130 T15128 pobj total,of
R4540 T15131 T15130 prep of,total
R4541 T15132 T15133 nummod 80,animals
R4542 T15133 T15131 pobj animals,of
R4543 T15134 T15133 amod genotyped,animals
R4544 T15135 T15127 punct ", ",were
R4545 T15136 T15137 nummod 22.5,%
R4546 T15137 T15127 nsubj %,were
R4547 T15138 T15139 amod wild,type
R4548 T15139 T15127 attr type,were
R4549 T15140 T15139 punct -,type
R4550 T15141 T15127 cc and,were
R4551 T15142 T15143 nummod 77.5,%
R4552 T15143 T15144 nsubj %,were
R4553 T15144 T15127 conj were,were
R4554 T15145 T15144 acomp heterozygous,were
R4555 T15146 T15127 punct ", ",were
R4556 T15147 T15148 mark with,observed
R4557 T15148 T15127 advcl observed,were
R4558 T15149 T15150 det no,progeny
R4559 T15150 T15148 nsubj progeny,observed
R4560 T15151 T15152 amod homozygous,null
R4561 T15152 T15150 amod null,progeny
R4562 T15153 T15154 punct [,32
R4563 T15154 T15148 parataxis 32,observed
R4564 T15155 T15154 punct ],32
R4565 T15156 T15127 punct .,were
R4566 T15158 T15159 det These,observations
R4567 T15159 T15160 nsubj observations,suggest
R4568 T15161 T15162 det a,advantage
R4569 T15162 T15160 dobj advantage,suggest
R4570 T15163 T15162 amod developmental,advantage
R4571 T15164 T15162 acl associated,advantage
R4572 T15165 T15164 prep with,associated
R4573 T15166 T15167 amod reduced,expression
R4574 T15167 T15165 pobj expression,with
R4575 T15168 T15167 compound calpain,expression
R4576 T15169 T15160 punct .,suggest
R4577 T15171 T15172 advmod Perhaps,reveal
R4578 T15173 T15174 amod future,studies
R4579 T15174 T15172 nsubj studies,reveal
R4580 T15175 T15172 aux will,reveal
R4581 T15176 T15177 det a,basis
R4582 T15177 T15172 dobj basis,reveal
R4583 T15178 T15177 amod mechanistic,basis
R4584 T15179 T15177 prep for,basis
R4585 T15180 T15179 pobj this,for
R4586 T15181 T15172 punct .,reveal

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T12390 11012-11020 CL:0002322 denotes ES cells
T12389 11003-11008 PR:000005015 denotes Capn2
T12388 10991-10996 PR:000005015 denotes Capn2
T12387 10972-10987 GO:0035822 denotes gene conversion
T12386 10972-10976 SO:0000704 denotes gene
T12385 10917-10938 CL:0000007 denotes early embryonic cells
T12384 10917-10932 UBERON:0019248 denotes early embryonic
T12383 10719-10725 SO:0001023 denotes allele
T12382 10713-10718 PR:000005022 denotes Capn4
T12381 10375-10381 SO:0001023 denotes allele
T12380 10336-10341 CL:0002322 denotes cells
T12379 10325-10327 _FRAGMENT denotes ES
T12378 10316-10321 PR:000005022 denotes Capn4
T12377 10261-10282 GO:0009790 denotes embryonic development
T12376 10255-10270 UBERON:0019248 denotes early embryonic
T12375 10198-10220 GO:0007059 denotes chromosome segregation
T12374 10145-10167 GO:0007059 denotes chromosome segregation
T12373 10101-10114 GO:0009566 denotes fertilization
T12372 10079-10094 GO:0072687 denotes meiotic spindle
T12371 10079-10086 GO:0007126 denotes meiotic
T12370 10072-10078 CL:0000023 denotes oocyte
T12369 9975-9991 GO:0060473 denotes cortical granule
T12368 9922-9929 CL:0000023 denotes oocytes
T12367 9910-9918 GO:0016020 denotes membrane
T12366 9901-9909 GO:0005938 denotes cortical
T12365 9820-9837 GO:0007340 denotes acrosome reaction
T12364 9820-9828 GO:0001669 denotes acrosome
T12363 9774-9781 CL:0000023 denotes oocytes
T12362 9759-9764 CL:0000019 denotes sperm
T12361 9752-9758 NCBITaxon:9989 denotes rodent
T12360 9675-9692 GO:0007340 denotes acrosome reaction
T12359 9675-9683 GO:0001669 denotes acrosome
T12358 9636-9647 GO:0005737 denotes cytoplasmic
T12357 9610-9616 CL:0000023 denotes oocyte
T12356 9589-9594 CL:0000019 denotes Sperm
T12355 9539-9546 PR:000003090 denotes p27kip1
T12354 9524-9533 PR:000005121 denotes cyclin D1
T12353 9518-9522 PR:000000118 denotes p107
T12352 9513-9516 PR:000003035 denotes p53
T12351 9481-9491 GO:0007049 denotes cell cycle
T12350 9407-9417 GO:0007049 denotes cell cycle
T12349 9391-9399 GO:0051318 denotes G1 stage
T12348 9268-9286 GO:0008283 denotes cell proliferation
T12347 9222-9227 PR:000005022 denotes Capn4
T12346 9020-9025 PR:000005022 denotes Capn4
T12345 8928-8949 GO:0009790 denotes embryonic development
T12344 8928-8937 UBERON:0000922 denotes embryonic
T12343 8881-8886 PR:000005022 denotes Capn4
T12342 8826-8840 GO:0045132 denotes during meiosis
T12341 8772-8794 _FRAGMENT denotes chromosome segregation
T12340 8772-8809 GO:0000070 denotes chromosome segregation during mitosis
T12339 8687-8705 GO:0008283 denotes cell proliferation
T12338 8526-8540 GO:0051301 denotes cell divisions
T12337 8416-8428 UBERON:0019252 denotes 8-cell stage
T12336 8401-8408 UBERON:0000922 denotes embryos
T12335 8392-8397 PR:000005015 denotes Capn2
T12334 8247-8252 PR:000005015 denotes Capn2
T12333 8211-8219 CL:0002322 denotes ES cells
T12332 8163-8170 UBERON:0000922 denotes embryos
T12331 8103-8115 UBERON:0019248 denotes early embryo
T12330 8024-8028 SO:0000704 denotes gene
T12329 7957-7966 UBERON:0000922 denotes embryonic
T12328 7911-7917 UBERON:0000085 denotes morula
T12327 7865-7872 UBERON:0000922 denotes embryos
T12326 7776-7785 UBERON:0000922 denotes embryonic
T12325 7667-7671 SO:0000704 denotes gene
T12324 7629-7641 GO:0007566 denotes implantation
T12323 7593-7598 PR:000005015 denotes Capn2
T12322 7572-7579 UBERON:0000922 denotes embryos
T12321 7559-7571 GO:0007566 denotes implantation
T12320 7426-7438 UBERON:0019252 denotes 8-cell stage
T12319 7400-7407 UBERON:0000922 denotes embryos
T12318 7340-7347 UBERON:0000922 denotes embryos
T12317 7331-7336 PR:000005015 denotes Capn2
T12316 7305-7317 GO:0007566 denotes implantation
T12315 7179-7191 UBERON:0019252 denotes 8-cell stage
T12314 7146-7167 GO:0009790 denotes embryonic development
T12313 7146-7155 UBERON:0000922 denotes embryonic
T12312 7084-7091 UBERON:0000922 denotes embryos
T12311 7075-7080 PR:000005015 denotes Capn2
T12310 6908-6915 NCBITaxon:33208 denotes animals
T12309 6899-6904 PR:000005009 denotes Capn1
T12308 6772-6784 CL:0000232 denotes erythrocytes
T12307 6758-6767 CL:0000233 denotes platelets
T12306 6582-6594 CL:0000232 denotes erythrocytes
T12305 6568-6577 CL:0000233 denotes Platelets
T12304 6411-6420 NCBITaxon:40674 denotes mammalian
T12303 6337-6356 GO:0030168 denotes platelet activation
T12302 6337-6345 CL:0000233 denotes platelet
T12301 6316-6324 GO:0006468 denotes proteins
T12300 6289-6307 _FRAGMENT denotes phosphorylation of
T12299 6232-6240 CL:0000233 denotes platelet
T12298 6189-6194 PR:000005009 denotes Capn1
T12297 6130-6134 NCBITaxon:10088 denotes mice
T12296 6091-6096 PR:000005009 denotes Capn1
T12295 6085-6089 SO:0000704 denotes gene
T12294 6061-6084 PR:000005009 denotes μ-calpain large subunit
T12293 6018-6030 GO:0007566 denotes implantation
T12292 6005-6008 GO:0016265 denotes die
T12291 5997-6004 UBERON:0000922 denotes embryos
T12290 5990-5996 NCBITaxon:39107 denotes murine
T12289 5979-5984 PR:000005015 denotes Capn2
T12288 5946-5959 GO:0009790 denotes embryogenesis
T12287 5862-5866 NCBITaxon:10088 denotes mice
T12286 5834-5838 SO:0000704 denotes gene
T12285 5784-5793 GO:0065007 denotes regulated
T12284 5756-5764 SO:0001060 denotes isoforms
T12283 5698-5702 CHEBI:29108 denotes Ca2+
T12282 5502-5508 MOP:0000780 denotes cleave
T12281 5326-5334 SO:0001060 denotes isoforms
T12280 5204-5208 NCBITaxon:10088 denotes mice
T12279 5090-5097 SO:0000704 denotes genetic
T12278 5029-5034 PR:000005022 denotes Capn4
T12277 4966-4975 NCBITaxon:40674 denotes mammalian
T12276 4952-4959 NCBITaxon:562 denotes E. coli
T12275 4926-4935 GO:0010467 denotes expressed
T12274 4749-4765 CL:0000255 denotes eukaryotic cells
T12273 4749-4759 NCBITaxon:2759 denotes eukaryotic
T12272 4586-4595 NCBITaxon:40674 denotes mammalian
T12271 4545-4552 NCBITaxon:562 denotes E. coli
T12248 3251-3257 SO:0001023 denotes allele
T12247 3172-3177 PR:000005022 denotes Capn4
T12246 3059-3064 SO:0000147 denotes exons
T12245 2954-2959 PR:000005022 denotes Capn4
T12244 2923-2930 NCBITaxon:562 denotes E. coli
T12243 2896-2919 PR:000005015 denotes m-calpain large subunit
T12242 2877-2886 GO:0010467 denotes expressed
T12241 2640-2644 SO:0000147 denotes exon
T12240 2612-2620 SO:0005853 denotes cassette
T12239 2562-2583 PR:000005022 denotes calpain small subunit
T12238 2461-2466 PR:000005022 denotes Capn4
T12237 2301-2310 GO:0007565 denotes gestation
T12236 2220-2227 UBERON:0000922 denotes embryos
T12235 2211-2216 PR:000005022 denotes Capn4
T12234 2163-2170 UBERON:0000922 denotes embryos
T12233 2132-2144 GO:0007566 denotes implantation
T12232 2099-2104 PR:000005022 denotes Capn4
T12231 1970-1974 SO:0000704 denotes gene
T12230 1964-1969 PR:000005022 denotes Capn4
T12229 1914-1927 GO:0009790 denotes embryogenesis
T12228 1789-1801 GO:0007566 denotes implantation
T12227 1775-1778 GO:0016265 denotes die
T12226 1746-1773 PR:000005015 denotes m-calpain catalytic subunit
T12225 1725-1729 NCBITaxon:10088 denotes mice
T12224 1716-1721 PR:000005015 denotes Capn2
T12223 1675-1684 UBERON:0000922 denotes embryonic
T12222 1631-1638 SO:0001060 denotes isoform
T12221 1601-1614 GO:0009790 denotes embryogenesis
T12220 1417-1437 GO:0070527 denotes platelet aggregation
T12219 1417-1425 CL:0000233 denotes platelet
T12218 1353-1380 PR:000005009 denotes μ-calpain catalytic subunit
T12217 1332-1336 NCBITaxon:10088 denotes mice
T12216 1323-1328 PR:000005009 denotes Capn1
T12215 1233-1240 SO:0001060 denotes isoform
T12214 1096-1104 SO:0001060 denotes isoforms
T12213 1065-1086 GO:0009790 denotes embryonic development
T12212 1065-1074 UBERON:0000922 denotes embryonic
T12211 825-829 NCBITaxon:10088 denotes mice
T12210 810-815 PR:000005022 denotes Capn4
T12209 787-796 UBERON:0000922 denotes embryonic
T12208 697-707 GO:0065007 denotes regulatory
T12207 672-676 SO:0000704 denotes gene
T12206 666-671 PR:000005022 denotes Capn4
T12205 621-629 SO:0001060 denotes isoforms
T12204 528-532 NCBITaxon:10088 denotes mice
T12203 510-514 SO:0000704 denotes Gene
T12202 458-467 GO:0065007 denotes regulated
T12201 424-432 SO:0001060 denotes isoforms
T12200 215-225 CHEBI:35222 denotes inhibitors
T12270 4532-4541 GO:0010467 denotes expressed
T12269 4416-4426 GO:0010467 denotes expression
T12268 4230-4241 SO:0000673 denotes transcripts
T12267 4200-4208 SO:0000167 denotes promoter
T12266 4176-4188 SO:0000162 denotes splice sites
T12265 4176-4182 GO:0008380 denotes splice
T12264 4168-4175 SO:0000976 denotes cryptic
T12263 4135-4139 SO:0000147 denotes exon
T12262 4031-4037 SO:0001023 denotes allele
T12261 4018-4024 SO:0001023 denotes allele
T12260 4012-4017 PR:000005022 denotes Capn4
T12259 3954-3965 SO:0000673 denotes transcripts
T12258 3902-3907 PR:000005022 denotes Capn4
T12257 3848-3855 SO:0001023 denotes alleles
T12256 3648-3661 GO:0009790 denotes embryogenesis
T12255 3632-3640 GO:0016265 denotes perished
T12254 3624-3631 UBERON:0000922 denotes embryos
T12253 3580-3584 SO:0000704 denotes gene
T12252 3575-3578 PR:000010445 denotes Mll
T12251 3551-3573 PR:000010445 denotes mixed lineage leukemia
T12250 3437-3441 SO:0000704 denotes gene
T12249 3316-3322 SO:0001023 denotes allele
T12396 11262-11271 SO:0000902 denotes transgene
T12395 11244-11249 PR:000005015 denotes Capn2
T12394 11222-11236 PR:000005015 denotes m-80 k subunit
T12393 11210-11217 GO:0010467 denotes express
T12392 11175-11183 CL:0002322 denotes ES cells
T12391 11070-11074 CHEBI:42768 denotes G418
T12407 12325-12335 GO:0010467 denotes expression
T12406 12134-12141 NCBITaxon:33208 denotes animals
T12405 11947-11952 PR:000005022 denotes Capn4
T12404 11803-11807 NCBITaxon:10088 denotes mice
T12403 11668-11673 PR:000005015 denotes Capn2
T12402 11648-11653 PR:000005015 denotes Capn2
T12401 11471-11480 SO:0000902 denotes transgene
T12400 11446-11456 GO:0010467 denotes expression
T12399 11390-11396 SO:0001023 denotes allele
T12398 11281-11296 GO:0035822 denotes gene conversion
T12397 11281-11285 SO:0000704 denotes gene
R2364 T12380 T12379 _lexicallyChainedTo cells,ES
R2359 T12301 T12300 _lexicallyChainedTo proteins,phosphorylation of
R2360 T12342 T12341 _lexicallyChainedTo during meiosis,chromosome segregation

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T12812 12134-12141 NCBITaxon:33208 denotes animals
T12811 12093-12101 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T12810 11953-11963 SO_EXT:transgenic_entity denotes transgenic
T12809 11947-11952 PR_EXT:000005022 denotes Capn4
T12808 11803-11807 NCBITaxon:10088 denotes mice
T12807 11776-11785 SO_EXT:wild_type_entity_or_quality denotes wild-type
T12806 11675-11676 SO_EXT:sequence_nullness_or_absence denotes -
T12805 11673-11674 SO_EXT:normal_or_wild_type_or_present denotes +
T12804 11668-11673 PR_EXT:000005015 denotes Capn2
T12803 11655-11656 SO_EXT:normal_or_wild_type_or_present denotes +
T12802 11653-11654 SO_EXT:normal_or_wild_type_or_present denotes +
T12801 11648-11653 PR_EXT:000005015 denotes Capn2
T12800 11601-11609 GO_EXT:breeding denotes breeding
T12799 11471-11480 SO_EXT:0000902 denotes transgene
T12798 11464-11470 SO_EXT:sequence_rescue_process denotes rescue
T12797 11446-11456 GO:0010467 denotes expression
T12796 11390-11396 SO_EXT:0001023 denotes allele
T12795 11380-11389 SO_EXT:wild_type_entity_or_quality denotes wild-type
T12794 11329-11338 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12793 11322-11328 SO_EXT:sequence_rescue_process denotes rescue
T12792 11281-11296 GO:0035822 denotes gene conversion
T12791 11281-11285 SO_EXT:0000704 denotes gene
T12790 11262-11271 SO_EXT:0000902 denotes transgene
T12789 11255-11261 SO_EXT:sequence_rescue_process denotes rescue
T12788 11250-11254 SO_EXT:cDNA denotes cDNA
T12787 11244-11249 PR_EXT:000005015 denotes Capn2
T12786 11222-11236 PR_EXT:000005015 denotes m-80 k subunit
T12785 11210-11217 GO:0010467 denotes express
T12784 11178-11183 CL_GO_EXT:cell denotes cells
T12783 11175-11183 CL:0002322 denotes ES cells
T12782 11155-11161 SO_EXT:sequence_cloned_entity denotes clonal
T12781 11137-11141 CL_GO_EXT:cell denotes cell
T12780 11104-11110 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T12779 11070-11074 CHEBI:42768 denotes G418
T12778 11037-11043 SO_EXT:sequence_cloned_entity denotes clones
T12777 11015-11020 CL_GO_EXT:cell denotes cells
T12776 11012-11020 CL:0002322 denotes ES cells
T12775 11010-11011 SO_EXT:sequence_nullness_or_absence denotes -
T12774 11008-11009 SO_EXT:sequence_nullness_or_absence denotes -
T12773 11003-11008 PR_EXT:000005015 denotes Capn2
T12772 10998-10999 SO_EXT:sequence_nullness_or_absence denotes -
T12771 10996-10997 SO_EXT:normal_or_wild_type_or_present denotes +
T12770 10991-10996 PR_EXT:000005015 denotes Capn2
T12769 10972-10987 GO:0035822 denotes gene conversion
T12768 10972-10976 SO_EXT:0000704 denotes gene
T12767 10933-10938 CL_GO_EXT:cell denotes cells
T12766 10917-10938 CL:0000007 denotes early embryonic cells
T12765 10917-10932 UBERON:0019248 denotes early embryonic
T12764 10887-10891 CL_GO_EXT:cell denotes cell
T12763 10851-10855 CL_GO_EXT:cell denotes cell
T12762 10785-10790 CL_GO_EXT:cell denotes cells
T12761 10719-10725 SO_EXT:0001023 denotes allele
T12760 10713-10718 PR_EXT:000005022 denotes Capn4
T12759 10706-10712 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T12758 10529-10537 GO_PR_EXT:spectrin denotes spectrin
T12757 10375-10381 SO_EXT:0001023 denotes allele
T12756 10336-10341 CL_GO_EXT:cell denotes cells
T12755 10336-10341 CL:0002322 denotes cells
T12754 10325-10327 _FRAGMENT denotes ES
T12753 10323-10324 SO_EXT:sequence_nullness_or_absence denotes -
T12752 10321-10322 SO_EXT:sequence_nullness_or_absence denotes -
T12751 10316-10321 PR_EXT:000005022 denotes Capn4
T12750 10261-10282 GO:0009790 denotes embryonic development
T12749 10255-10270 UBERON:0019248 denotes early embryonic
T12748 10237-10243 GO_EXT:fatality_or_lethality denotes lethal
T12747 10198-10220 GO:0007059 denotes chromosome segregation
T12746 10198-10208 GO_SO_EXT:chromosome denotes chromosome
T12745 10145-10167 GO:0007059 denotes chromosome segregation
T12744 10145-10155 GO_SO_EXT:chromosome denotes chromosome
T12743 10101-10114 GO:0009566 denotes fertilization
T12742 10079-10094 GO:0072687 denotes meiotic spindle
T12725 9752-9758 NCBITaxon:9989 denotes rodent
T12724 9711-9720 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12723 9713-9720 GO_PR_EXT:calpain_1_complex denotes calpain
T12722 9704-9706 _FRAGMENT denotes μ-
T12721 9675-9692 GO:0007340 denotes acrosome reaction
T12720 9675-9683 GO:0001669 denotes acrosome
T12719 9648-9655 CHEBI_EXT:calcium denotes calcium
T12718 9636-9647 GO:0005737 denotes cytoplasmic
T12717 9610-9616 CL:0000023 denotes oocyte
T12716 9595-9602 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12715 9589-9594 CL:0000019 denotes Sperm
T12714 9539-9546 PR_EXT:000003090 denotes p27kip1
T12713 9524-9533 PR_EXT:000005121 denotes cyclin D1
T12712 9518-9522 PR_EXT:000000118 denotes p107
T12711 9513-9516 PR_EXT:000003035 denotes p53
T12710 9481-9491 GO:0007049 denotes cell cycle
T12709 9481-9485 CL_GO_EXT:cell denotes cell
T12708 9448-9456 CHEBI_PR_EXT:protein denotes proteins
T12707 9407-9417 GO:0007049 denotes cell cycle
T12706 9407-9411 CL_GO_EXT:cell denotes cell
T12705 9391-9399 GO:0051318 denotes G1 stage
T12704 9309-9313 CL_GO_EXT:cell denotes cell
T12703 9268-9286 GO:0008283 denotes cell proliferation
T12702 9268-9272 CL_GO_EXT:cell denotes cell
T12701 9229-9230 SO_EXT:sequence_nullness_or_absence denotes -
T12700 9227-9228 SO_EXT:sequence_nullness_or_absence denotes -
T12699 9222-9227 PR_EXT:000005022 denotes Capn4
T12698 9212-9217 CL_GO_EXT:cell denotes cells
T12697 9129-9138 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12696 9131-9138 GO_PR_EXT:calpain_1_complex denotes calpain
T12695 9122-9124 _FRAGMENT denotes μ-
T12694 9044-9048 CL_GO_EXT:cell denotes cell
T12693 9027-9028 SO_EXT:sequence_nullness_or_absence denotes -
T12692 9025-9026 SO_EXT:sequence_nullness_or_absence denotes -
T12691 9020-9025 PR_EXT:000005022 denotes Capn4
T12690 8987-8991 CL_GO_EXT:cell denotes cell
T12689 8928-8949 GO:0009790 denotes embryonic development
T12688 8928-8937 UBERON:0000922 denotes embryonic
T12687 8890-8895 CL_GO_EXT:cell denotes cells
T12686 8888-8889 SO_EXT:sequence_nullness_or_absence denotes -
T12685 8886-8887 SO_EXT:sequence_nullness_or_absence denotes -
T12684 8881-8886 PR_EXT:000005022 denotes Capn4
T12683 8858-8867 GO_EXT:biological_movement_or_translocation_process denotes migration
T12682 8826-8840 GO:0045132 denotes during meiosis
T12681 8772-8794 _FRAGMENT denotes chromosome segregation
T12680 8772-8809 GO:0000070 denotes chromosome segregation during mitosis
T12679 8772-8782 GO_SO_EXT:chromosome denotes chromosome
T12678 8687-8705 GO:0008283 denotes cell proliferation
T12677 8687-8691 CL_GO_EXT:cell denotes cell
T12676 8662-8671 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12675 8598-8607 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12674 8570-8579 GO_EXT:fatality_or_lethality denotes lethality
T12673 8526-8540 GO:0051301 denotes cell divisions
T12672 8526-8530 CL_GO_EXT:cell denotes cell
T12671 8506-8513 CHEBI_PR_EXT:protein denotes protein
T12670 8494-8498 CHEBI_SO_EXT:mRNA denotes mRNA
T12669 8484-8493 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12668 8418-8422 CL_GO_EXT:cell denotes cell
T12667 8416-8428 UBERON:0019252 denotes 8-cell stage
T12666 8401-8408 UBERON:0000922 denotes embryos
T12665 8399-8400 SO_EXT:sequence_nullness_or_absence denotes -
T12664 8397-8398 SO_EXT:sequence_nullness_or_absence denotes -
T12663 8392-8397 PR_EXT:000005015 denotes Capn2
T12662 8354-8358 CL_GO_EXT:cell denotes cell
T12661 8312-8321 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12660 8247-8252 PR_EXT:000005015 denotes Capn2
T12659 8214-8219 CL_GO_EXT:cell denotes cells
T12658 8211-8219 CL:0002322 denotes ES cells
T12657 8204-8210 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T12656 8163-8170 UBERON:0000922 denotes embryos
T12655 8158-8162 SO_EXT:sequence_nullness denotes null
T12654 8103-8115 UBERON:0019248 denotes early embryo
T12653 8090-8095 CL_GO_EXT:cell denotes cells
T12652 8065-8072 GO_EXT:fatality_or_lethality denotes fatally
T12651 8024-8028 SO_EXT:0000704 denotes gene
T12650 7967-7976 SO_EXT:genotype_or_entity_with_genotype denotes genotypes
T12649 7957-7966 UBERON:0000922 denotes embryonic
T12648 7911-7917 UBERON:0000085 denotes morula
T12647 7865-7872 UBERON:0000922 denotes embryos
T12646 7860-7864 SO_EXT:sequence_nullness denotes Null
T12645 7786-7801 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T12644 7776-7785 UBERON:0000922 denotes embryonic
T12643 7667-7671 SO_EXT:0000704 denotes gene
T12642 7642-7651 GO_EXT:fatality_or_lethality denotes lethality
T12641 7629-7641 GO:0007566 denotes implantation
T12640 7600-7601 SO_EXT:sequence_nullness_or_absence denotes -
T12639 7598-7599 SO_EXT:sequence_nullness_or_absence denotes -
T12638 7593-7598 PR_EXT:000005015 denotes Capn2
T12637 7572-7579 UBERON:0000922 denotes embryos
T12636 7559-7571 GO:0007566 denotes implantation
T12635 7428-7432 CL_GO_EXT:cell denotes cell
T12634 7426-7438 UBERON:0019252 denotes 8-cell stage
T12633 7400-7407 UBERON:0000922 denotes embryos
T12632 7395-7399 SO_EXT:sequence_nullness denotes null
T12631 7340-7347 UBERON:0000922 denotes embryos
T12630 7338-7339 SO_EXT:sequence_nullness_or_absence denotes -
T12629 7336-7337 SO_EXT:sequence_nullness_or_absence denotes -
T12628 7331-7336 PR_EXT:000005015 denotes Capn2
T12627 7318-7327 GO_EXT:fatality_or_lethality denotes lethality
T12626 7305-7317 GO:0007566 denotes implantation
T12625 7250-7259 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12624 7238-7245 GO_PR_EXT:calpain_1_complex denotes calpain
T12623 7236-7245 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12622 7181-7185 CL_GO_EXT:cell denotes cell
T12621 7179-7191 UBERON:0019252 denotes 8-cell stage
T12620 7146-7167 GO:0009790 denotes embryonic development
T12619 7146-7155 UBERON:0000922 denotes embryonic
T12618 7110-7119 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12617 7084-7091 UBERON:0000922 denotes embryos
T12616 7082-7083 SO_EXT:sequence_nullness_or_absence denotes -
T12615 7080-7081 SO_EXT:sequence_nullness_or_absence denotes -
T12614 7075-7080 PR_EXT:000005015 denotes Capn2
T12613 7062-7071 GO_EXT:fatality_or_lethality denotes lethality
T12612 7013-7022 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12611 6965-6974 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12610 6908-6915 NCBITaxon:33208 denotes animals
T12609 6906-6907 SO_EXT:sequence_nullness_or_absence denotes -
T12608 6904-6905 SO_EXT:sequence_nullness_or_absence denotes -
T12607 6899-6904 PR_EXT:000005009 denotes Capn1
T12606 6799-6803 CL_GO_EXT:cell denotes cell
T12605 6772-6784 CL:0000232 denotes erythrocytes
T12604 6758-6767 CL:0000233 denotes platelets
T12603 6720-6729 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12602 6696-6705 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12601 6638-6647 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12600 6612-6621 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12599 6582-6594 CL:0000232 denotes erythrocytes
T12598 6568-6577 CL:0000233 denotes Platelets
T12597 6421-6426 CL_GO_EXT:cell denotes cells
T12596 6411-6420 NCBITaxon:40674 denotes mammalian
T12595 6370-6379 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12594 6372-6379 GO_PR_EXT:calpain_1_complex denotes calpain
T12593 6363-6365 _FRAGMENT denotes μ-
T12592 6337-6356 GO:0030168 denotes platelet activation
T12591 6337-6345 CL:0000233 denotes platelet
T12590 6316-6324 CHEBI_PR_EXT:protein denotes proteins
T12589 6316-6324 GO:0006468 denotes proteins
T12588 6289-6307 _FRAGMENT denotes phosphorylation of
T12587 6280-6288 CHEBI_SO_EXT:tyrosine denotes tyrosine
T12586 6232-6240 CL:0000233 denotes platelet
T12585 6189-6194 PR_EXT:000005009 denotes Capn1
T12584 6130-6134 NCBITaxon:10088 denotes mice
T12583 6091-6096 PR_EXT:000005009 denotes Capn1
T12582 6085-6089 SO_EXT:0000704 denotes gene
T12581 6063-6070 GO_PR_EXT:calpain_1_complex denotes calpain
T12580 6061-6084 PR_EXT:000005009 denotes μ-calpain large subunit
T12579 6018-6030 GO:0007566 denotes implantation
T12578 6005-6008 GO:0016265 denotes die
T12577 5997-6004 UBERON:0000922 denotes embryos
T12576 5990-5996 NCBITaxon:39107 denotes murine
T12575 5985-5989 SO_EXT:sequence_nullness denotes null
T12574 5979-5984 PR_EXT:000005015 denotes Capn2
T12573 5946-5959 GO:0009790 denotes embryogenesis
T12572 5909-5922 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T12571 5880-5889 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12570 5882-5889 GO_PR_EXT:calpain_1_complex denotes calpain
T12569 5873-5875 _FRAGMENT denotes μ-
T12568 5862-5866 NCBITaxon:10088 denotes mice
T12567 5834-5838 SO_EXT:0000704 denotes gene
T12566 5801-5806 CL_GO_EXT:cell denotes cells
T12565 5784-5793 GO:0065007 denotes regulated
T12564 5756-5764 SO_EXT:0001060 denotes isoforms
T12563 5698-5702 CHEBI:29108 denotes Ca2+
T12562 5502-5508 MOP:0000780 denotes cleave
T12561 5412-5420 SO_EXT:biological_sequence denotes sequence
T12560 5394-5401 CHEBI_GO_SO_EXT:enzyme denotes enzymes
T12559 5343-5352 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12558 5345-5352 GO_PR_EXT:calpain_1_complex denotes calpain
T12557 5336-5338 _FRAGMENT denotes μ-
T12556 5326-5334 SO_EXT:0001060 denotes isoforms
T12555 5204-5208 NCBITaxon:10088 denotes mice
T12554 5193-5203 SO_EXT:transgenic_entity denotes transgenic
T12553 5121-5131 SO_EXT:transgenic_entity denotes transgenic
T12552 5090-5097 SO_EXT:0000704 denotes genetic
T12551 5029-5034 PR_EXT:000005022 denotes Capn4
T12550 5012-5021 GO_EXT:fatality_or_lethality denotes lethality
T12548 4966-4975 NCBITaxon:40674 denotes mammalian
T12547 4952-4959 NCBITaxon:562 denotes E. coli
T12546 4926-4935 GO:0010467 denotes expressed
T12545 4760-4765 CL_GO_EXT:cell denotes cells
T12544 4749-4765 CL:0000255 denotes eukaryotic cells
T12543 4749-4759 NCBITaxon:2759 denotes eukaryotic
T12542 4596-4601 CL_GO_EXT:cell denotes cells
T12541 4586-4595 NCBITaxon:40674 denotes mammalian
T12540 4545-4552 NCBITaxon:562 denotes E. coli
T12539 4532-4541 GO:0010467 denotes expressed
T12538 4450-4458 SO_EXT:sequence_alteration_process denotes modified
T12537 4416-4426 GO:0010467 denotes expression
T12536 4330-4335 CHEBI_SO_EXT:amino_acid denotes acids
T12535 4319-4329 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T12534 4230-4241 SO_EXT:0000673 denotes transcripts
T12533 4209-4217 SO_EXT:biological_sequence denotes sequence
T12532 4200-4208 SO_EXT:0000167 denotes promoter
T12531 4196-4199 GO_EXT:phosphoglycerate_kinase denotes PGK
T12530 4176-4188 SO_EXT:0000162 denotes splice sites
T12529 4176-4182 GO:0008380 denotes splice
T12528 4168-4175 SO:0000976 denotes cryptic
T12527 4135-4139 SO_EXT:0000147 denotes exon
T12526 4088-4090 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T12525 4060-4064 CHEBI_SO_EXT:mRNA denotes mRNA
T12524 4031-4037 SO_EXT:0001023 denotes allele
T12523 4018-4024 SO_EXT:0001023 denotes allele
T12522 4012-4017 PR_EXT:000005022 denotes Capn4
T12521 3954-3965 SO_EXT:0000673 denotes transcripts
T12520 3908-3918 SO_EXT:transgenic_entity denotes transgenic
T12519 3902-3907 PR_EXT:000005022 denotes Capn4
T12518 3848-3855 SO_EXT:0001023 denotes alleles
T12517 3841-3847 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T12516 3814-3822 CHEBI_PR_EXT:protein denotes proteins
T12515 3804-3813 SO_EXT:sequence_truncation_process denotes truncated
T12514 3648-3661 GO:0009790 denotes embryogenesis
T12513 3632-3640 GO:0016265 denotes perished
T12512 3624-3631 UBERON:0000922 denotes embryos
T12511 3619-3623 SO_EXT:sequence_nullness denotes null
T12510 3580-3584 SO_EXT:0000704 denotes gene
T12741 10079-10086 GO:0007126 denotes meiotic
T12740 10072-10078 CL:0000023 denotes oocyte
T12739 10055-10064 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T12738 10038-10047 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12737 9975-9991 GO:0060473 denotes cortical granule
T12736 9964-9971 GO_EXT:biological_release denotes release
T12735 9922-9929 CL:0000023 denotes oocytes
T12734 9910-9918 GO:0016020 denotes membrane
T12733 9901-9909 GO:0005938 denotes cortical
T12732 9884-9893 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12731 9867-9880 GO_EXT:biological_translocation_entity_or_process denotes translocation
T12730 9820-9837 GO:0007340 denotes acrosome reaction
T12729 9820-9828 GO:0001669 denotes acrosome
T12728 9788-9797 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12727 9774-9781 CL:0000023 denotes oocytes
T12726 9759-9764 CL:0000019 denotes sperm
T12509 3575-3578 PR_EXT:000010445 denotes Mll
T12508 3551-3573 PR_EXT:000010445 denotes mixed lineage leukemia
T12507 3437-3441 SO_EXT:0000704 denotes gene
T12506 3347-3355 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T12505 3316-3322 SO_EXT:0001023 denotes allele
T12504 3290-3298 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T12503 3285-3289 SO_EXT:sequence_nullness denotes null
T12502 3251-3257 SO_EXT:0001023 denotes allele
T12501 3172-3177 PR_EXT:000005022 denotes Capn4
T12500 3140-3149 GO_EXT:fatality_or_lethality denotes lethality
T12499 3059-3064 SO_EXT:0000147 denotes exons
T12498 3047-3055 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T12497 2954-2959 PR_EXT:000005022 denotes Capn4
T12496 2923-2930 NCBITaxon:562 denotes E. coli
T12495 2896-2919 PR_EXT:000005015 denotes m-calpain large subunit
T12494 2896-2905 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12493 2877-2886 GO:0010467 denotes expressed
T12492 2812-2820 CHEBI_EXT:residue denotes residues
T12491 2801-2811 CHEBI_SO_EXT:amino_acid denotes amino acid
T12490 2787-2797 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T12489 2771-2779 SO_EXT:sequence_deletion_process denotes excision
T12488 2679-2686 CHEBI_PR_EXT:protein denotes protein
T12487 2661-2671 SO_EXT:sequence_truncation_entity_or_process denotes truncation
T12486 2640-2644 SO_EXT:0000147 denotes exon
T12485 2612-2620 SO_EXT:0005853 denotes cassette
T12484 2608-2611 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T12483 2604-2607 GO_EXT:phosphoglycerate_kinase denotes PGK
T12482 2587-2596 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T12481 2562-2583 PR_EXT:000005022 denotes calpain small subunit
T12480 2544-2554 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T12479 2461-2466 PR_EXT:000005022 denotes Capn4
T12478 2451-2455 SO_EXT:null_sequence_entity denotes null
T12477 2301-2310 GO:0007565 denotes gestation
T12476 2220-2227 UBERON:0000922 denotes embryos
T12475 2218-2219 SO_EXT:sequence_nullness_or_absence denotes -
T12474 2216-2217 SO_EXT:sequence_nullness_or_absence denotes -
T12473 2211-2216 PR_EXT:000005022 denotes Capn4
T12472 2163-2170 UBERON:0000922 denotes embryos
T12471 2158-2162 SO_EXT:sequence_nullness denotes null
T12470 2145-2154 GO_EXT:fatality_or_lethality denotes lethality
T12469 2132-2144 GO:0007566 denotes implantation
T12468 2099-2104 PR_EXT:000005022 denotes Capn4
T12467 2004-2013 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12466 1970-1974 SO_EXT:0000704 denotes gene
T12465 1964-1969 PR_EXT:000005022 denotes Capn4
T12464 1914-1927 GO:0009790 denotes embryogenesis
T12463 1872-1881 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12462 1789-1801 GO:0007566 denotes implantation
T12461 1775-1778 GO:0016265 denotes die
T12460 1756-1765 GO_MOP_EXT:catalysis denotes catalytic
T12459 1746-1773 PR_EXT:000005015 denotes m-calpain catalytic subunit
T12458 1746-1755 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12457 1725-1729 NCBITaxon:10088 denotes mice
T12456 1723-1724 SO_EXT:sequence_nullness_or_absence denotes -
T12455 1721-1722 SO_EXT:sequence_nullness_or_absence denotes -
T12454 1716-1721 PR_EXT:000005015 denotes Capn2
T12453 1675-1684 UBERON:0000922 denotes embryonic
T12452 1631-1638 SO_EXT:0001060 denotes isoform
T12451 1601-1614 GO:0009790 denotes embryogenesis
T12450 1558-1567 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12449 1417-1437 GO:0070527 denotes platelet aggregation
T12448 1417-1425 CL:0000233 denotes platelet
T12447 1363-1372 GO_MOP_EXT:catalysis denotes catalytic
T12446 1353-1380 PR_EXT:000005009 denotes μ-calpain catalytic subunit
T12445 1353-1362 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12444 1332-1336 NCBITaxon:10088 denotes mice
T12443 1330-1331 SO_EXT:sequence_nullness_or_absence denotes -
T12442 1328-1329 SO_EXT:sequence_nullness_or_absence denotes -
T12441 1323-1328 PR_EXT:000005009 denotes Capn1
T12440 1233-1240 SO_EXT:0001060 denotes isoform
T12439 1187-1196 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12438 1189-1196 GO_PR_EXT:calpain_1_complex denotes calpain
T12437 1180-1182 _FRAGMENT denotes μ-
T12436 1150-1159 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12435 1123-1132 GO_PR_EXT:calpain_1_complex denotes μ-calpain
T12434 1096-1104 SO_EXT:0001060 denotes isoforms
T12433 1065-1086 GO:0009790 denotes embryonic development
T12432 1065-1074 UBERON:0000922 denotes embryonic
T12431 977-986 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12430 979-986 GO_PR_EXT:calpain_1_complex denotes calpain
T12429 970-972 _FRAGMENT denotes μ-
T12428 825-829 NCBITaxon:10088 denotes mice
T12427 810-815 PR_EXT:000005022 denotes Capn4
T12426 797-806 GO_EXT:fatality_or_lethality denotes lethality
T12425 787-796 UBERON:0000922 denotes embryonic
T12424 738-747 GO_PR_EXT:calpain_2_complex denotes m-calpain
T12423 740-747 GO_PR_EXT:calpain_1_complex denotes calpain
T12422 731-733 _FRAGMENT denotes μ-
T12421 697-707 GO:0065007 denotes regulatory
T12420 678-686 SO_EXT:sequence_coding_function denotes encoding
T12419 672-676 SO_EXT:0000704 denotes gene
T12418 666-671 PR_EXT:000005022 denotes Capn4
T12417 621-629 SO_EXT:0001060 denotes isoforms
T12416 581-592 GO_PATO_EXT:physiological_process_or_quality denotes physiologic
T12415 528-532 NCBITaxon:10088 denotes mice
T12414 510-514 SO_EXT:0000704 denotes Gene
T12413 458-467 GO:0065007 denotes regulated
T12412 424-432 SO_EXT:0001060 denotes isoforms
T12411 303-312 GO_EXT:0008233 denotes proteases
T12410 250-265 GO_EXT:negative_regulation denotes down-regulating
T12409 215-225 CHEBI_EXT:35222 denotes inhibitors
T12408 137-144 CHEBI_GO_SO_EXT:enzyme denotes enzymes
T12549 4976-4981 CL_GO_EXT:cell denotes cells
T12815 12325-12335 GO:0010467 denotes expression
T12814 12212-12216 SO_EXT:sequence_nullness denotes null
T12813 12154-12163 SO_EXT:wild_type_entity_or_quality denotes wild-type
R2358 T12423 T12422 _lexicallyChainedTo calpain,μ-
R2361 T12430 T12429 _lexicallyChainedTo calpain,μ-
R2362 T12438 T12437 _lexicallyChainedTo calpain,μ-
R2363 T12558 T12557 _lexicallyChainedTo calpain,μ-
R2365 T12570 T12569 _lexicallyChainedTo calpain,μ-
R2366 T12589 T12588 _lexicallyChainedTo proteins,phosphorylation of
R2367 T12594 T12593 _lexicallyChainedTo calpain,μ-
R2368 T12755 T12754 _lexicallyChainedTo cells,ES
R2369 T12682 T12681 _lexicallyChainedTo during meiosis,chromosome segregation
R2371 T12696 T12695 _lexicallyChainedTo calpain,μ-
R2372 T12723 T12722 _lexicallyChainedTo calpain,μ-