Id |
Subject |
Object |
Predicate |
Lexical cue |
T15137 |
12147-12148 |
NN |
denotes |
% |
T15136 |
12143-12147 |
CD |
denotes |
22.5 |
T15135 |
12141-12143 |
, |
denotes |
, |
T15134 |
12124-12133 |
VBN |
denotes |
genotyped |
T15133 |
12134-12141 |
NNS |
denotes |
animals |
T15132 |
12121-12123 |
CD |
denotes |
80 |
T15131 |
12118-12120 |
IN |
denotes |
of |
T15130 |
12112-12117 |
NN |
denotes |
total |
T15129 |
12110-12111 |
DT |
denotes |
a |
T15128 |
12107-12109 |
IN |
denotes |
of |
T15127 |
12149-12153 |
VBD |
denotes |
were |
T15126 |
12103-12106 |
IN |
denotes |
Out |
T15125 |
12102-12239 |
sentence |
denotes |
Out of a total of 80 genotyped animals, 22.5% were wild-type and 77.5% were heterozygous, with no homozygous null progeny observed [32]. |
T15124 |
12101-12102 |
. |
denotes |
. |
T15123 |
12080-12092 |
JJ |
denotes |
heterozygous |
T15122 |
12093-12101 |
NN |
denotes |
genotype |
T15121 |
12076-12079 |
DT |
denotes |
the |
T15120 |
12073-12075 |
IN |
denotes |
of |
T15119 |
12067-12072 |
NN |
denotes |
favor |
T15118 |
12064-12066 |
IN |
denotes |
in |
T15117 |
12054-12056 |
, |
denotes |
, |
T15116 |
12042-12046 |
RBR |
denotes |
less |
T15115 |
12039-12041 |
IN |
denotes |
if |
T15114 |
12047-12054 |
JJ |
denotes |
extreme |
T15113 |
12037-12039 |
, |
denotes |
, |
T15112 |
12030-12037 |
JJ |
denotes |
similar |
T15111 |
12056-12063 |
VBG |
denotes |
skewing |
T15110 |
12028-12029 |
DT |
denotes |
a |
T15109 |
12023-12027 |
IN |
denotes |
with |
T15108 |
12015-12022 |
NN |
denotes |
progeny |
T15107 |
11996-12006 |
NNS |
denotes |
colleagues |
T15106 |
11992-11995 |
CC |
denotes |
and |
T15105 |
11982-11991 |
NNP |
denotes |
Zimmerman |
T15104 |
11979-11981 |
IN |
denotes |
by |
T15103 |
11969-11978 |
VBN |
denotes |
generated |
T15102 |
11953-11963 |
JJ |
denotes |
transgenic |
T15101 |
11947-11952 |
NN |
denotes |
Capn4 |
T15100 |
11964-11968 |
NN |
denotes |
line |
T15099 |
11943-11946 |
DT |
denotes |
the |
T15098 |
11933-11942 |
VBG |
denotes |
involving |
T15097 |
11925-11932 |
NNS |
denotes |
crosses |
T15096 |
11912-11924 |
JJ |
denotes |
heterozygous |
T15095 |
11910-11912 |
, |
denotes |
, |
T15094 |
12007-12014 |
VBD |
denotes |
yielded |
T15093 |
11897-11910 |
RB |
denotes |
Interestingly |
T15092 |
11896-12102 |
sentence |
denotes |
Interestingly, heterozygous crosses involving the Capn4 transgenic line generated by Zimmerman and colleagues yielded progeny with a similar, if less extreme, skewing in favor of the heterozygous genotype. |
T15091 |
11895-11896 |
. |
denotes |
. |
T15090 |
11886-11895 |
NN |
denotes |
offspring |
T15089 |
11873-11885 |
JJ |
denotes |
heterozygous |
T15088 |
11870-11872 |
IN |
denotes |
of |
T15087 |
11850-11858 |
VBN |
denotes |
expected |
T15086 |
11845-11849 |
IN |
denotes |
than |
T15085 |
11837-11844 |
JJR |
denotes |
greater |
T15084 |
11859-11869 |
NN |
denotes |
proportion |
T15083 |
11835-11836 |
DT |
denotes |
a |
T15082 |
11830-11834 |
IN |
denotes |
with |
T15081 |
11822-11829 |
NN |
denotes |
progeny |
T15080 |
11808-11812 |
RB |
denotes |
also |
T15079 |
11790-11802 |
JJ |
denotes |
heterozygous |
T15078 |
11786-11789 |
CC |
denotes |
and |
T15077 |
11803-11807 |
NNS |
denotes |
mice |
T15076 |
11780-11781 |
HYPH |
denotes |
- |
T15075 |
11781-11785 |
NN |
denotes |
type |
T15074 |
11776-11780 |
JJ |
denotes |
wild |
T15073 |
11768-11775 |
IN |
denotes |
between |
T15072 |
11813-11821 |
VBD |
denotes |
produced |
T15071 |
11760-11767 |
NNS |
denotes |
Crosses |
T15070 |
11759-11896 |
sentence |
denotes |
Crosses between wild-type and heterozygous mice also produced progeny with a greater than expected proportion of heterozygous offspring. |
T15069 |
11758-11759 |
. |
denotes |
. |
T15068 |
11752-11758 |
NN |
denotes |
result |
T15067 |
11747-11751 |
DT |
denotes |
this |
T15066 |
11743-11746 |
IN |
denotes |
for |
T15065 |
11723-11730 |
JJ |
denotes |
obvious |
T15064 |
11731-11742 |
NN |
denotes |
explanation |
T15063 |
11720-11722 |
DT |
denotes |
no |
T15062 |
11711-11716 |
EX |
denotes |
there |
T15061 |
11709-11711 |
, |
denotes |
, |
T15060 |
11702-11709 |
JJ |
denotes |
present |
T15059 |
11717-11719 |
VBZ |
denotes |
is |
T15058 |
11699-11701 |
IN |
denotes |
At |
T15057 |
11698-11759 |
sentence |
denotes |
At present, there is no obvious explanation for this result. |
T15056 |
11697-11698 |
. |
denotes |
. |
T15055 |
11688-11697 |
NNS |
denotes |
weanlings |
T15054 |
11685-11687 |
IN |
denotes |
of |
T15053 |
11683-11684 |
-RRB- |
denotes |
) |
T15052 |
11678-11682 |
CD |
denotes |
88.4 |
T15051 |
11682-11683 |
NN |
denotes |
% |
T15050 |
11677-11678 |
-LRB- |
denotes |
( |
T15049 |
11675-11676 |
SYM |
denotes |
- |
T15048 |
11674-11675 |
HYPH |
denotes |
/ |
T15047 |
11673-11674 |
SYM |
denotes |
+ |
T15046 |
11668-11673 |
NN |
denotes |
Capn2 |
T15045 |
11665-11667 |
IN |
denotes |
to |
T15044 |
11663-11664 |
-RRB- |
denotes |
) |
T15043 |
11658-11662 |
CD |
denotes |
11.6 |
T15042 |
11662-11663 |
NN |
denotes |
% |
T15041 |
11657-11658 |
-LRB- |
denotes |
( |
T15040 |
11655-11656 |
SYM |
denotes |
+ |
T15039 |
11654-11655 |
HYPH |
denotes |
/ |
T15038 |
11653-11654 |
SYM |
denotes |
+ |
T15037 |
11648-11653 |
NN |
denotes |
Capn2 |
T15036 |
11645-11647 |
IN |
denotes |
of |
T15035 |
11625-11638 |
JJ |
denotes |
non-Mendelian |
T15034 |
11618-11624 |
RB |
denotes |
highly |
T15033 |
11639-11644 |
NN |
denotes |
ratio |
T15032 |
11614-11617 |
DT |
denotes |
the |
T15031 |
11596-11609 |
NN |
denotes |
interbreeding |
T15030 |
11583-11595 |
JJ |
denotes |
heterozygous |
T15029 |
11578-11582 |
IN |
denotes |
from |
T15028 |
11570-11577 |
NN |
denotes |
progeny |
T15027 |
11567-11569 |
IN |
denotes |
of |
T15026 |
11556-11566 |
NN |
denotes |
genotyping |
T15025 |
11552-11555 |
DT |
denotes |
the |
T15024 |
11545-11551 |
IN |
denotes |
during |
T15023 |
11539-11544 |
VBD |
denotes |
arose |
T15022 |
11533-11538 |
WDT |
denotes |
which |
T15021 |
11610-11613 |
VBD |
denotes |
was |
T15020 |
11523-11532 |
NN |
denotes |
curiosity |
T15019 |
11519-11522 |
CD |
denotes |
One |
T15018 |
11518-11698 |
sentence |
denotes |
One curiosity which arose during the genotyping of progeny from heterozygous interbreeding was the highly non-Mendelian ratio of Capn2+/+ (11.6%) to Capn2+/- (88.4%) of weanlings. |
T15017 |
11517-11518 |
. |
denotes |
. |
T15016 |
11509-11517 |
NNS |
denotes |
attempts |
T15015 |
11503-11508 |
DT |
denotes |
these |
T15014 |
11490-11493 |
RB |
denotes |
far |
T15013 |
11485-11489 |
RB |
denotes |
thus |
T15012 |
11481-11484 |
VBZ |
denotes |
has |
T15011 |
11464-11470 |
NN |
denotes |
rescue |
T15010 |
11471-11480 |
NN |
denotes |
transgene |
T15009 |
11460-11463 |
DT |
denotes |
the |
T15008 |
11457-11459 |
IN |
denotes |
of |
T15007 |
11446-11456 |
NN |
denotes |
expression |
T15006 |
11439-11445 |
JJ |
denotes |
stable |
T15005 |
11429-11438 |
VBG |
denotes |
achieving |
T15004 |
11426-11428 |
IN |
denotes |
in |
T15003 |
11415-11425 |
NN |
denotes |
difficulty |
T15002 |
11413-11415 |
, |
denotes |
, |
T15001 |
11494-11502 |
VBN |
denotes |
hampered |
T15000 |
11406-11413 |
RB |
denotes |
However |
T14999 |
11405-11518 |
sentence |
denotes |
However, difficulty in achieving stable expression of the rescue transgene has thus far hampered these attempts. |
T14998 |
11404-11405 |
. |
denotes |
. |
T14997 |
11397-11399 |
VBZ |
denotes |
is |
T14996 |
11384-11385 |
HYPH |
denotes |
- |
T14995 |
11385-11389 |
NN |
denotes |
type |
T14994 |
11380-11384 |
JJ |
denotes |
wild |
T14993 |
11369-11379 |
JJ |
denotes |
endogenous |
T14992 |
11359-11368 |
VBG |
denotes |
remaining |
T14991 |
11390-11396 |
NN |
denotes |
allele |
T14990 |
11355-11358 |
DT |
denotes |
the |
T14989 |
11400-11404 |
VBN |
denotes |
lost |
T14988 |
11348-11354 |
IN |
denotes |
before |
T14987 |
11339-11347 |
NN |
denotes |
activity |
T14986 |
11330-11331 |
HYPH |
denotes |
- |
T14985 |
11331-11338 |
NN |
denotes |
calpain |
T14984 |
11329-11330 |
NN |
denotes |
m |
T14983 |
11309-11321 |
RB |
denotes |
preemptively |
T14982 |
11322-11328 |
VB |
denotes |
rescue |
T14981 |
11306-11308 |
TO |
denotes |
to |
T14980 |
11300-11305 |
NN |
denotes |
order |
T14979 |
11297-11299 |
IN |
denotes |
in |
T14978 |
11286-11296 |
NN |
denotes |
conversion |
T14977 |
11281-11285 |
NN |
denotes |
gene |
T14976 |
11278-11280 |
IN |
denotes |
to |
T14975 |
11272-11277 |
JJ |
denotes |
prior |
T14974 |
11255-11261 |
NN |
denotes |
rescue |
T14973 |
11250-11254 |
NN |
denotes |
cDNA |
T14972 |
11244-11249 |
NN |
denotes |
Capn2 |
T14971 |
11262-11271 |
NN |
denotes |
transgene |
T14970 |
11242-11243 |
DT |
denotes |
a |
T14969 |
11237-11241 |
IN |
denotes |
from |
T14968 |
11227-11228 |
NN |
denotes |
k |
T14967 |
11224-11226 |
CD |
denotes |
80 |
T14966 |
11223-11224 |
HYPH |
denotes |
- |
T14965 |
11222-11223 |
NN |
denotes |
m |
T14964 |
11229-11236 |
NN |
denotes |
subunit |
T14963 |
11218-11221 |
DT |
denotes |
the |
T14962 |
11210-11217 |
VB |
denotes |
express |
T14961 |
11207-11209 |
TO |
denotes |
to |
T14960 |
11203-11206 |
RB |
denotes |
way |
T14959 |
11197-11202 |
RB |
denotes |
under |
T14958 |
11193-11196 |
VBP |
denotes |
are |
T14957 |
11185-11192 |
NNS |
denotes |
Efforts |
T14956 |
11184-11405 |
sentence |
denotes |
Efforts are under way to express the m-80 k subunit from a Capn2 cDNA rescue transgene prior to gene conversion in order to preemptively rescue m-calpain activity before the remaining endogenous wild-type allele is lost. |
T14955 |
11183-11184 |
. |
denotes |
. |
T14954 |
11178-11183 |
NNS |
denotes |
cells |
T14953 |
11175-11177 |
NN |
denotes |
ES |
T14952 |
11172-11174 |
IN |
denotes |
of |
T14951 |
11162-11171 |
NN |
denotes |
selection |
T14950 |
11155-11161 |
JJ |
denotes |
clonal |
T14949 |
11152-11154 |
CC |
denotes |
or |
T14948 |
11142-11151 |
NN |
denotes |
viability |
T14947 |
11137-11141 |
NN |
denotes |
cell |
T14946 |
11117-11124 |
RB |
denotes |
somehow |
T14945 |
11104-11110 |
NN |
denotes |
mutant |
T14885 |
10791-10939 |
sentence |
denotes |
Furthermore, calpain-independent mechanisms for protecting cell viability might exist in these cell lines that are absent in early embryonic cells. |
T14884 |
10790-10791 |
. |
denotes |
. |
T14883 |
10785-10790 |
NNS |
denotes |
cells |
T14882 |
10781-10784 |
DT |
denotes |
the |
T14881 |
10778-10780 |
IN |
denotes |
of |
T14880 |
10768-10777 |
NN |
denotes |
viability |
T14879 |
10764-10767 |
DT |
denotes |
the |
T14878 |
10752-10763 |
VBG |
denotes |
maintaining |
T14877 |
10748-10751 |
IN |
denotes |
for |
T14876 |
10737-10747 |
JJ |
denotes |
sufficient |
T14875 |
10734-10736 |
VBZ |
denotes |
is |
T14874 |
10731-10733 |
PRP |
denotes |
it |
T14873 |
10727-10730 |
CC |
denotes |
and |
T14872 |
10725-10727 |
, |
denotes |
, |
T14871 |
10713-10718 |
NN |
denotes |
Capn4 |
T14870 |
10706-10712 |
NN |
denotes |
mutant |
T14869 |
10719-10725 |
NN |
denotes |
allele |
T14868 |
10701-10705 |
DT |
denotes |
this |
T14867 |
10696-10700 |
IN |
denotes |
from |
T14866 |
10684-10686 |
VBZ |
denotes |
is |
T14865 |
10682-10684 |
, |
denotes |
, |
T14864 |
10675-10682 |
NNS |
denotes |
methods |
T14863 |
10669-10674 |
DT |
denotes |
these |
T14862 |
10666-10668 |
IN |
denotes |
by |
T14861 |
10655-10665 |
JJ |
denotes |
detectable |
T14860 |
10648-10654 |
NNS |
denotes |
levels |
T14859 |
10640-10647 |
IN |
denotes |
beneath |
T14858 |
10638-10640 |
, |
denotes |
, |
T14857 |
10630-10638 |
NN |
denotes |
activity |
T14856 |
10622-10629 |
NN |
denotes |
calpain |
T14855 |
10619-10621 |
IN |
denotes |
of |
T14854 |
10606-10611 |
NN |
denotes |
trace |
T14853 |
10612-10618 |
NN |
denotes |
amount |
T14852 |
10604-10605 |
DT |
denotes |
a |
T14851 |
10687-10695 |
VBN |
denotes |
retained |
T14850 |
10599-10603 |
IN |
denotes |
that |
T14849 |
10597-10599 |
, |
denotes |
, |
T14848 |
10590-10597 |
RBR |
denotes |
earlier |
T14847 |
10580-10589 |
VBN |
denotes |
discussed |
T14846 |
10577-10579 |
IN |
denotes |
as |
T14845 |
10575-10577 |
, |
denotes |
, |
T14844 |
10564-10575 |
JJ |
denotes |
conceivable |
T14843 |
10561-10563 |
VBZ |
denotes |
is |
T14842 |
10558-10560 |
PRP |
denotes |
It |
T14841 |
10557-10791 |
sentence |
denotes |
It is conceivable, as discussed earlier, that a trace amount of calpain activity, beneath levels detectable by these methods, is retained from this mutant Capn4 allele, and it is sufficient for maintaining the viability of the cells. |
T14840 |
10556-10557 |
. |
denotes |
. |
T14839 |
10538-10547 |
NN |
denotes |
breakdown |
T14838 |
10529-10537 |
NN |
denotes |
spectrin |
T14837 |
10548-10556 |
NNS |
denotes |
products |
T14836 |
10514-10528 |
JJ |
denotes |
characteristic |
T14835 |
10511-10513 |
IN |
denotes |
of |
T14834 |
10500-10510 |
NN |
denotes |
appearance |
T14833 |
10496-10499 |
DT |
denotes |
the |
T14832 |
10493-10495 |
IN |
denotes |
by |
T14831 |
10490-10492 |
CC |
denotes |
or |
T14830 |
10479-10489 |
NN |
denotes |
zymography |
T14829 |
10472-10478 |
NN |
denotes |
casein |
T14828 |
10469-10471 |
IN |
denotes |
by |
T14827 |
10460-10468 |
VBN |
denotes |
assessed |
T14826 |
10457-10459 |
IN |
denotes |
as |
T14825 |
10455-10457 |
, |
denotes |
, |
T14824 |
10447-10455 |
NN |
denotes |
activity |
T14823 |
10439-10446 |
NN |
denotes |
calpain |
T14822 |
10436-10438 |
IN |
denotes |
of |
T14821 |
10422-10430 |
JJ |
denotes |
apparent |
T14820 |
10431-10435 |
NN |
denotes |
lack |
T14819 |
10419-10421 |
DT |
denotes |
an |
T14818 |
10411-10418 |
IN |
denotes |
despite |
T14817 |
10403-10410 |
NN |
denotes |
culture |
T14816 |
10400-10402 |
IN |
denotes |
in |
T14815 |
10386-10388 |
VB |
denotes |
be |
T14814 |
10382-10385 |
MD |
denotes |
can |
T14813 |
10363-10374 |
JJ |
denotes |
hypomorphic |
T14812 |
10351-10362 |
JJ |
denotes |
presumptive |
T14811 |
10375-10381 |
NN |
denotes |
allele |
T14810 |
10347-10350 |
DT |
denotes |
the |
T14809 |
10342-10346 |
IN |
denotes |
from |
T14808 |
10332-10335 |
NN |
denotes |
MEF |
T14807 |
10328-10331 |
CC |
denotes |
and |
T14806 |
10325-10327 |
NN |
denotes |
ES |
T14805 |
10323-10324 |
SYM |
denotes |
- |
T14804 |
10322-10323 |
HYPH |
denotes |
/ |
T14803 |
10321-10322 |
SYM |
denotes |
- |
T14802 |
10336-10341 |
NNS |
denotes |
cells |
T14801 |
10316-10321 |
NN |
denotes |
Capn4 |
T14800 |
10389-10399 |
VBN |
denotes |
maintained |
T14799 |
10311-10315 |
IN |
denotes |
that |
T14798 |
10299-10301 |
VB |
denotes |
be |
T14797 |
10294-10298 |
RB |
denotes |
also |
T14796 |
10287-10293 |
MD |
denotes |
should |
T14795 |
10302-10310 |
VBN |
denotes |
stressed |
T14794 |
10284-10286 |
PRP |
denotes |
It |
T14793 |
10283-10557 |
sentence |
denotes |
It should also be stressed that Capn4-/- ES and MEF cells from the presumptive hypomorphic allele can be maintained in culture despite an apparent lack of calpain activity, as assessed by casein zymography or by the appearance of characteristic spectrin breakdown products. |
T14792 |
10282-10283 |
. |
denotes |
. |
T14791 |
10261-10270 |
JJ |
denotes |
embryonic |
T14790 |
10271-10282 |
NN |
denotes |
development |
T14789 |
10255-10260 |
JJ |
denotes |
early |
T14788 |
10252-10254 |
IN |
denotes |
on |
T14787 |
10244-10251 |
NNS |
denotes |
effects |
T14786 |
10237-10243 |
JJ |
denotes |
lethal |
T14785 |
10227-10231 |
RB |
denotes |
both |
T14784 |
10221-10226 |
MD |
denotes |
would |
T14783 |
10198-10208 |
NN |
denotes |
chromosome |
T14782 |
10209-10220 |
NN |
denotes |
segregation |
T14781 |
10188-10197 |
JJ |
denotes |
defective |
T14780 |
10185-10187 |
CC |
denotes |
or |
T14779 |
10232-10236 |
VB |
denotes |
have |
T14778 |
10174-10184 |
NN |
denotes |
Polyspermy |
T14777 |
10173-10283 |
sentence |
denotes |
Polyspermy or defective chromosome segregation would both have lethal effects on early embryonic development. |
T14776 |
10172-10173 |
. |
denotes |
. |
T14775 |
10171-10172 |
-RRB- |
denotes |
] |
T14774 |
10169-10171 |
CD |
denotes |
45 |
T14773 |
10168-10169 |
-LRB- |
denotes |
[ |
T14772 |
10156-10167 |
NN |
denotes |
segregation |
T14771 |
10145-10155 |
NN |
denotes |
chromosome |
T14770 |
10142-10144 |
IN |
denotes |
in |
T14769 |
10130-10132 |
VB |
denotes |
be |
T14768 |
10124-10129 |
MD |
denotes |
could |
T14767 |
10121-10123 |
PRP |
denotes |
it |
T14766 |
10133-10141 |
VBN |
denotes |
involved |
T14765 |
10116-10120 |
VBD |
denotes |
were |
T14764 |
10114-10116 |
, |
denotes |
, |
T14763 |
10101-10114 |
NN |
denotes |
fertilization |
T14762 |
10095-10100 |
IN |
denotes |
after |
T14761 |
10079-10086 |
JJ |
denotes |
meiotic |
T14760 |
10072-10078 |
NN |
denotes |
oocyte |
T14759 |
10087-10094 |
NN |
denotes |
spindle |
T14758 |
10068-10071 |
DT |
denotes |
the |
T14757 |
10065-10067 |
IN |
denotes |
to |
T14756 |
10048-10052 |
RB |
denotes |
also |
T14755 |
10053-10064 |
VBD |
denotes |
relocalized |
T14754 |
10039-10040 |
HYPH |
denotes |
- |
T14753 |
10040-10047 |
NN |
denotes |
calpain |
T14752 |
10038-10039 |
NN |
denotes |
m |
T14751 |
10037-10173 |
sentence |
denotes |
m-calpain also relocalized to the oocyte meiotic spindle after fertilization, were it could be involved in chromosome segregation [45]. |
T14750 |
10036-10037 |
. |
denotes |
. |
T14749 |
10035-10036 |
-RRB- |
denotes |
] |
T14748 |
10033-10035 |
CD |
denotes |
45 |
T14747 |
10032-10033 |
-LRB- |
denotes |
[ |
T14746 |
10021-10031 |
NN |
denotes |
polyspermy |
T14745 |
10013-10020 |
VB |
denotes |
prevent |
T14744 |
10010-10012 |
TO |
denotes |
to |
T14743 |
10001-10009 |
VBN |
denotes |
required |
T14742 |
9984-9991 |
NN |
denotes |
granule |
T14741 |
9992-10000 |
NNS |
denotes |
contents |
T14740 |
9975-9983 |
JJ |
denotes |
cortical |
T14739 |
9972-9974 |
IN |
denotes |
of |
T14738 |
9964-9971 |
NN |
denotes |
release |
T14737 |
9960-9963 |
DT |
denotes |
the |
T14736 |
9957-9959 |
IN |
denotes |
in |
T14735 |
9939-9944 |
MD |
denotes |
might |
T14734 |
9936-9938 |
PRP |
denotes |
it |
T14733 |
9945-9956 |
VB |
denotes |
participate |
T14732 |
9930-9935 |
WRB |
denotes |
where |
T14731 |
9922-9929 |
NNS |
denotes |
oocytes |
T14730 |
9919-9921 |
IN |
denotes |
in |
T14729 |
9901-9909 |
JJ |
denotes |
cortical |
T14728 |
9910-9918 |
NN |
denotes |
membrane |
T14727 |
9897-9900 |
DT |
denotes |
the |
T14726 |
9894-9896 |
IN |
denotes |
to |
T14725 |
9885-9886 |
HYPH |
denotes |
- |
T14724 |
9886-9893 |
NN |
denotes |
calpain |
T14723 |
9884-9885 |
NN |
denotes |
m |
T14722 |
9881-9883 |
IN |
denotes |
of |
T14721 |
9867-9880 |
NN |
denotes |
translocation |
T14720 |
9865-9866 |
DT |
denotes |
a |
T14719 |
9860-9864 |
IN |
denotes |
with |
T14718 |
9849-9859 |
VBD |
denotes |
correlated |
T14717 |
9843-9848 |
WDT |
denotes |
which |
T14716 |
9841-9842 |
-RRB- |
denotes |
] |
T14715 |
9839-9841 |
CD |
denotes |
47 |
T14714 |
9838-9839 |
-LRB- |
denotes |
[ |
T14713 |
9820-9828 |
NN |
denotes |
acrosome |
T14712 |
9829-9837 |
NN |
denotes |
reaction |
T14711 |
9816-9819 |
DT |
denotes |
the |
T14710 |
9813-9815 |
IN |
denotes |
in |
T14709 |
9798-9801 |
VBD |
denotes |
was |
T14708 |
9789-9790 |
HYPH |
denotes |
- |
T14707 |
9790-9797 |
NN |
denotes |
calpain |
T14706 |
9788-9789 |
NN |
denotes |
m |
T14705 |
9786-9787 |
. |
denotes |
. |
T15161 |
12267-12268 |
DT |
denotes |
a |
T14704 |
9785-9786 |
-RRB- |
denotes |
] |
T14703 |
9783-9785 |
CD |
denotes |
45 |
T14702 |
9782-9783 |
-LRB- |
denotes |
[ |
T14701 |
9774-9781 |
NNS |
denotes |
oocytes |
T14700 |
9770-9773 |
CC |
denotes |
and |
T14699 |
9768-9769 |
-RRB- |
denotes |
] |
T14698 |
9766-9768 |
CD |
denotes |
47 |
T14697 |
9765-9766 |
-LRB- |
denotes |
[ |
T14696 |
9759-9764 |
NN |
denotes |
sperm |
T14695 |
9752-9758 |
NN |
denotes |
rodent |
T14694 |
9749-9751 |
IN |
denotes |
in |
T14693 |
9802-9812 |
VBN |
denotes |
implicated |
T14692 |
9735-9739 |
VBN |
denotes |
been |
T14691 |
9726-9734 |
RB |
denotes |
recently |
T14690 |
9721-9725 |
VBP |
denotes |
have |
T14689 |
9740-9748 |
VBN |
denotes |
detected |
T14688 |
9712-9713 |
HYPH |
denotes |
- |
T14687 |
9711-9712 |
NN |
denotes |
m |
T14686 |
9707-9710 |
CC |
denotes |
and |
T14685 |
9705-9706 |
HYPH |
denotes |
- |
T14684 |
9713-9720 |
NN |
denotes |
calpain |
T14683 |
9704-9705 |
NN |
denotes |
μ |
T14682 |
9699-9703 |
CC |
denotes |
Both |
T14681 |
9698-10037 |
sentence |
denotes |
Both μ- and m-calpain have recently been detected in rodent sperm [47] and oocytes [45]. m-calpain was implicated in the acrosome reaction [47] which correlated with a translocation of m-calpain to the cortical membrane in oocytes where it might participate in the release of cortical granule contents required to prevent polyspermy [45]. |
T14680 |
9697-9698 |
. |
denotes |
. |
T14679 |
9696-9697 |
-RRB- |
denotes |
] |
T14678 |
9694-9696 |
CD |
denotes |
46 |
T14677 |
9693-9694 |
-LRB- |
denotes |
[ |
T14676 |
9675-9683 |
NN |
denotes |
acrosome |
T14675 |
9684-9692 |
NN |
denotes |
reaction |
T14674 |
9671-9674 |
DT |
denotes |
the |
T14673 |
9662-9670 |
VBZ |
denotes |
triggers |
T14672 |
9656-9661 |
WDT |
denotes |
which |
T14671 |
9636-9647 |
JJ |
denotes |
cytoplasmic |
T14670 |
9648-9655 |
NN |
denotes |
calcium |
T14609 |
9346-9350 |
VBN |
denotes |
been |
T14608 |
9341-9345 |
RB |
denotes |
also |
T14607 |
9337-9340 |
VBZ |
denotes |
has |
T14606 |
9351-9361 |
VBN |
denotes |
associated |
T14605 |
9329-9336 |
NN |
denotes |
Calpain |
T14604 |
9328-9422 |
sentence |
denotes |
Calpain has also been associated with progression through the G1 stage of the cell cycle [6]. |
T14603 |
9327-9328 |
. |
denotes |
. |
T14602 |
9326-9327 |
-RRB- |
denotes |
] |
T14601 |
9325-9326 |
CD |
denotes |
5 |
T14600 |
9324-9325 |
-LRB- |
denotes |
[ |
T14599 |
9309-9313 |
NN |
denotes |
cell |
T14598 |
9314-9323 |
NNS |
denotes |
densities |
T14597 |
9305-9308 |
JJ |
denotes |
low |
T14596 |
9300-9304 |
RB |
denotes |
very |
T14595 |
9292-9296 |
RB |
denotes |
only |
T14594 |
9297-9299 |
IN |
denotes |
at |
T14593 |
9288-9291 |
CC |
denotes |
but |
T14592 |
9286-9288 |
, |
denotes |
, |
T14591 |
9273-9286 |
NN |
denotes |
proliferation |
T14590 |
9268-9272 |
NN |
denotes |
cell |
T14589 |
9258-9267 |
VB |
denotes |
influence |
T14588 |
9255-9257 |
TO |
denotes |
to |
T14587 |
9245-9248 |
VBD |
denotes |
was |
T14586 |
9237-9244 |
NN |
denotes |
calpain |
T14585 |
9235-9237 |
, |
denotes |
, |
T14584 |
9229-9230 |
SYM |
denotes |
- |
T14583 |
9228-9229 |
HYPH |
denotes |
/ |
T14582 |
9227-9228 |
SYM |
denotes |
- |
T14581 |
9231-9235 |
NNS |
denotes |
MEFs |
T14580 |
9222-9227 |
NN |
denotes |
Capn4 |
T14579 |
9218-9221 |
CC |
denotes |
and |
T14578 |
9208-9211 |
NN |
denotes |
CHO |
T14577 |
9212-9217 |
NNS |
denotes |
cells |
T14576 |
9203-9207 |
CC |
denotes |
both |
T14575 |
9249-9254 |
VBN |
denotes |
shown |
T14574 |
9200-9202 |
IN |
denotes |
In |
T14573 |
9199-9328 |
sentence |
denotes |
In both CHO cells and Capn4-/- MEFs, calpain was shown to influence cell proliferation, but only at very low cell densities [5]. |
T14572 |
9198-9199 |
. |
denotes |
. |
T14571 |
9197-9198 |
-RRB- |
denotes |
] |
T14570 |
9195-9196 |
, |
denotes |
, |
T14569 |
9194-9195 |
CD |
denotes |
5 |
T14568 |
9196-9197 |
CD |
denotes |
6 |
T14567 |
9193-9194 |
-LRB- |
denotes |
[ |
T14566 |
9183-9192 |
JJ |
denotes |
essential |
T14565 |
9174-9182 |
RB |
denotes |
strictly |
T14564 |
9162-9169 |
NN |
denotes |
calpain |
T14563 |
9170-9173 |
VBD |
denotes |
was |
T14562 |
9157-9161 |
IN |
denotes |
that |
T14561 |
9148-9151 |
RB |
denotes |
not |
T14560 |
9152-9156 |
VB |
denotes |
show |
T14559 |
9144-9147 |
VBD |
denotes |
did |
T14558 |
9140-9143 |
CC |
denotes |
and |
T14557 |
9138-9140 |
, |
denotes |
, |
T14556 |
9130-9131 |
HYPH |
denotes |
- |
T14555 |
9129-9130 |
NN |
denotes |
m |
T14554 |
9125-9128 |
CC |
denotes |
and |
T14553 |
9123-9124 |
HYPH |
denotes |
- |
T14552 |
9131-9138 |
NN |
denotes |
calpain |
T14551 |
9122-9123 |
NN |
denotes |
μ |
T14550 |
9114-9121 |
IN |
denotes |
between |
T14549 |
9098-9101 |
RB |
denotes |
not |
T14548 |
9094-9097 |
VBD |
denotes |
did |
T14547 |
9083-9093 |
RB |
denotes |
frequently |
T14546 |
9069-9077 |
VBN |
denotes |
reported |
T14545 |
9078-9082 |
NN |
denotes |
work |
T14544 |
9065-9068 |
DT |
denotes |
the |
T14543 |
9102-9113 |
VB |
denotes |
distinguish |
T14542 |
9056-9064 |
IN |
denotes |
although |
T14541 |
9054-9056 |
, |
denotes |
, |
T14540 |
9044-9048 |
NN |
denotes |
cell |
T14539 |
9049-9054 |
NNS |
denotes |
lines |
T14538 |
9038-9043 |
JJ |
denotes |
other |
T14537 |
9034-9037 |
CC |
denotes |
and |
T14536 |
9027-9028 |
SYM |
denotes |
- |
T14535 |
9026-9027 |
HYPH |
denotes |
/ |
T14534 |
9025-9026 |
SYM |
denotes |
- |
T14533 |
9029-9033 |
NNS |
denotes |
MEFs |
T14532 |
9020-9025 |
NN |
denotes |
Capn4 |
T14531 |
9017-9019 |
IN |
denotes |
in |
T14530 |
9006-9010 |
VBN |
denotes |
been |
T14529 |
9002-9005 |
VBZ |
denotes |
has |
T14528 |
8992-9001 |
NN |
denotes |
viability |
T14527 |
8987-8991 |
NN |
denotes |
cell |
T14526 |
8983-8986 |
CC |
denotes |
and |
T14525 |
8975-8982 |
NN |
denotes |
calpain |
T14524 |
8967-8974 |
IN |
denotes |
between |
T14523 |
9011-9016 |
VBN |
denotes |
noted |
T14522 |
8955-8966 |
NN |
denotes |
association |
T14521 |
8951-8954 |
DT |
denotes |
The |
T14520 |
8950-9199 |
sentence |
denotes |
The association between calpain and cell viability has been noted in Capn4-/- MEFs and other cell lines, although the reported work frequently did not distinguish between μ- and m-calpain, and did not show that calpain was strictly essential [5,6]. |
T14519 |
8949-8950 |
. |
denotes |
. |
T14518 |
8928-8937 |
JJ |
denotes |
embryonic |
T14517 |
8938-8949 |
NN |
denotes |
development |
T14516 |
8921-8927 |
VBN |
denotes |
failed |
T14515 |
8918-8920 |
IN |
denotes |
to |
T14514 |
8901-8906 |
MD |
denotes |
could |
T14513 |
8899-8901 |
, |
denotes |
, |
T14512 |
8898-8899 |
-RRB- |
denotes |
] |
T14511 |
8897-8898 |
CD |
denotes |
9 |
T14510 |
8896-8897 |
-LRB- |
denotes |
[ |
T14509 |
8888-8889 |
SYM |
denotes |
- |
T14508 |
8887-8888 |
HYPH |
denotes |
/ |
T14507 |
8886-8887 |
SYM |
denotes |
- |
T14506 |
8890-8895 |
NNS |
denotes |
cells |
T14505 |
8881-8886 |
NN |
denotes |
Capn4 |
T14504 |
8878-8880 |
IN |
denotes |
in |
T14503 |
8869-8877 |
VBN |
denotes |
reported |
T14502 |
8867-8869 |
, |
denotes |
, |
T14501 |
8858-8867 |
NN |
denotes |
migration |
T14500 |
8855-8857 |
IN |
denotes |
in |
T14499 |
8907-8917 |
VB |
denotes |
contribute |
T14498 |
8847-8854 |
NNS |
denotes |
Defects |
T14497 |
8846-8950 |
sentence |
denotes |
Defects in migration, reported in Capn4-/- cells [9], could contribute to failed embryonic development. |
T14496 |
8845-8846 |
. |
denotes |
. |
T14495 |
8844-8845 |
-RRB- |
denotes |
] |
T14494 |
8842-8844 |
CD |
denotes |
45 |
T14493 |
8841-8842 |
-LRB- |
denotes |
[ |
T14492 |
8833-8840 |
NN |
denotes |
meiosis |
T14491 |
8826-8832 |
IN |
denotes |
during |
T14490 |
8818-8822 |
RB |
denotes |
well |
T14489 |
8823-8825 |
IN |
denotes |
as |
T14488 |
8815-8817 |
RB |
denotes |
as |
T14487 |
8813-8814 |
-RRB- |
denotes |
] |
T14486 |
8811-8813 |
CD |
denotes |
44 |
T14485 |
8810-8811 |
-LRB- |
denotes |
[ |
T14484 |
8802-8809 |
NN |
denotes |
mitosis |
T14483 |
8795-8801 |
IN |
denotes |
during |
T14482 |
8783-8794 |
NN |
denotes |
segregation |
T14481 |
8772-8782 |
NN |
denotes |
chromosome |
T14480 |
8769-8771 |
IN |
denotes |
in |
T14479 |
8757-8768 |
NN |
denotes |
involvement |
T14478 |
8753-8756 |
PRP$ |
denotes |
its |
T14477 |
8750-8752 |
IN |
denotes |
of |
T14476 |
8742-8749 |
NNS |
denotes |
reports |
T14475 |
8732-8741 |
VBG |
denotes |
including |
T14474 |
8730-8732 |
, |
denotes |
, |
T14473 |
8717-8730 |
NNS |
denotes |
circumstances |
T14472 |
8709-8716 |
JJ |
denotes |
certain |
T14471 |
8706-8708 |
IN |
denotes |
in |
T14470 |
8692-8705 |
NN |
denotes |
proliferation |
T14469 |
8687-8691 |
NN |
denotes |
cell |
T14468 |
8684-8686 |
IN |
denotes |
in |
T14467 |
8672-8683 |
NN |
denotes |
involvement |
T14466 |
8663-8664 |
HYPH |
denotes |
- |
T14465 |
8664-8671 |
NN |
denotes |
calpain |
T14464 |
8662-8663 |
NN |
denotes |
m |
T14463 |
8659-8661 |
IN |
denotes |
of |
T14462 |
8651-8658 |
NNS |
denotes |
reports |
T14461 |
8643-8650 |
JJ |
denotes |
several |
T14460 |
8633-8637 |
VBP |
denotes |
have |
T14459 |
8638-8642 |
VBN |
denotes |
been |
T14458 |
8627-8632 |
EX |
denotes |
There |
T14457 |
8626-8846 |
sentence |
denotes |
There have been several reports of m-calpain involvement in cell proliferation in certain circumstances, including reports of its involvement in chromosome segregation during mitosis [44] as well as during meiosis [45]. |
T14456 |
8625-8626 |
. |
denotes |
. |
T14455 |
8618-8625 |
JJ |
denotes |
unclear |
T14454 |
8612-8617 |
RB |
denotes |
still |
T14453 |
8599-8600 |
HYPH |
denotes |
- |
T14452 |
8600-8607 |
NN |
denotes |
calpain |
T14451 |
8598-8599 |
NN |
denotes |
m |
T14450 |
8595-8597 |
IN |
denotes |
of |
T14449 |
8587-8594 |
NN |
denotes |
absence |
T14448 |
8583-8586 |
DT |
denotes |
the |
T14447 |
8580-8582 |
IN |
denotes |
in |
T14446 |
8570-8579 |
NN |
denotes |
lethality |
T14445 |
8566-8569 |
IN |
denotes |
for |
T14444 |
8608-8611 |
VBP |
denotes |
are |
T14443 |
8546-8557 |
JJ |
denotes |
mechanistic |
T14442 |
8558-8565 |
NNS |
denotes |
reasons |
T14441 |
8542-8545 |
DT |
denotes |
The |
T14440 |
8541-8626 |
sentence |
denotes |
The mechanistic reasons for lethality in the absence of m-calpain are still unclear. |
T14439 |
8540-8541 |
. |
denotes |
. |
T14438 |
8526-8530 |
NN |
denotes |
cell |
T14437 |
8531-8540 |
NNS |
denotes |
divisions |
T14436 |
8523-8524 |
SYM |
denotes |
– |
T14435 |
8524-8525 |
CD |
denotes |
3 |
T14434 |
8522-8523 |
CD |
denotes |
2 |
T14433 |
8514-8521 |
IN |
denotes |
through |
T14432 |
8506-8513 |
NN |
denotes |
protein |
T14431 |
8503-8505 |
CC |
denotes |
or |
T14430 |
8502-8503 |
HYPH |
denotes |
/ |
T14429 |
8499-8502 |
CC |
denotes |
and |
T14944 |
11093-11103 |
JJ |
denotes |
homozygous |
T14943 |
11111-11116 |
NN |
denotes |
state |
T14942 |
11089-11092 |
DT |
denotes |
the |
T14941 |
11125-11136 |
VBZ |
denotes |
compromises |
T14940 |
11084-11088 |
IN |
denotes |
that |
T14939 |
11070-11074 |
NN |
denotes |
G418 |
T14938 |
11067-11069 |
IN |
denotes |
of |
T14937 |
11052-11066 |
NNS |
denotes |
concentrations |
T14936 |
11047-11051 |
JJ |
denotes |
high |
T14935 |
11044-11046 |
IN |
denotes |
in |
T14934 |
11037-11043 |
NNS |
denotes |
clones |
T14933 |
11034-11036 |
IN |
denotes |
of |
T14932 |
11024-11033 |
NN |
denotes |
selection |
T14931 |
11021-11023 |
IN |
denotes |
by |
T14930 |
11015-11020 |
NNS |
denotes |
cells |
T14929 |
11012-11014 |
NN |
denotes |
ES |
T14928 |
11010-11011 |
SYM |
denotes |
- |
T14927 |
11009-11010 |
HYPH |
denotes |
/ |
T14926 |
11008-11009 |
SYM |
denotes |
- |
T14925 |
11003-11008 |
NN |
denotes |
Capn2 |
T14924 |
11000-11002 |
IN |
denotes |
to |
T14923 |
10998-10999 |
SYM |
denotes |
- |
T14922 |
10997-10998 |
HYPH |
denotes |
/ |
T14921 |
10996-10997 |
SYM |
denotes |
+ |
T14920 |
10991-10996 |
NN |
denotes |
Capn2 |
T14919 |
10988-10990 |
IN |
denotes |
of |
T14918 |
10977-10987 |
NN |
denotes |
conversion |
T14917 |
10972-10976 |
NN |
denotes |
gene |
T14916 |
10964-10971 |
VB |
denotes |
achieve |
T14915 |
10961-10963 |
TO |
denotes |
to |
T14914 |
11075-11083 |
VBZ |
denotes |
suggests |
T14913 |
10944-10952 |
VBN |
denotes |
repeated |
T14912 |
10953-10960 |
NN |
denotes |
failure |
T14911 |
10940-10943 |
DT |
denotes |
The |
T14910 |
10939-11184 |
sentence |
denotes |
The repeated failure to achieve gene conversion of Capn2+/- to Capn2-/- ES cells by selection of clones in high concentrations of G418 suggests that the homozygous mutant state somehow compromises cell viability or clonal selection of ES cells. |
T14909 |
10938-10939 |
. |
denotes |
. |
T14908 |
10923-10932 |
JJ |
denotes |
embryonic |
T14907 |
10933-10938 |
NNS |
denotes |
cells |
T14906 |
10917-10922 |
JJ |
denotes |
early |
T14905 |
10914-10916 |
IN |
denotes |
in |
T14904 |
10907-10913 |
JJ |
denotes |
absent |
T14903 |
10903-10906 |
VBP |
denotes |
are |
T14902 |
10898-10902 |
WDT |
denotes |
that |
T14901 |
10887-10891 |
NN |
denotes |
cell |
T14900 |
10892-10897 |
NNS |
denotes |
lines |
T14899 |
10881-10886 |
DT |
denotes |
these |
T14898 |
10878-10880 |
IN |
denotes |
in |
T14897 |
10866-10871 |
MD |
denotes |
might |
T14896 |
10856-10865 |
NN |
denotes |
viability |
T14895 |
10851-10855 |
NN |
denotes |
cell |
T14894 |
10840-10850 |
VBG |
denotes |
protecting |
T14893 |
10836-10839 |
IN |
denotes |
for |
T14892 |
10825-10835 |
NNS |
denotes |
mechanisms |
T14891 |
10812-10813 |
HYPH |
denotes |
- |
T14890 |
10813-10824 |
JJ |
denotes |
independent |
T14889 |
10805-10812 |
NN |
denotes |
calpain |
T14888 |
10803-10805 |
, |
denotes |
, |
T14887 |
10872-10877 |
VB |
denotes |
exist |
T14886 |
10792-10803 |
RB |
denotes |
Furthermore |
T14428 |
8494-8498 |
NN |
denotes |
mRNA |
T14427 |
8485-8486 |
HYPH |
denotes |
- |
T14426 |
8484-8485 |
NN |
denotes |
m |
T14425 |
8475-8483 |
JJ |
denotes |
maternal |
T14424 |
8486-8493 |
NN |
denotes |
calpain |
T14423 |
8470-8474 |
DT |
denotes |
some |
T14422 |
8467-8469 |
IN |
denotes |
of |
T14421 |
8455-8466 |
NN |
denotes |
persistence |
T14420 |
8451-8454 |
DT |
denotes |
the |
T14419 |
8448-8450 |
IN |
denotes |
to |
T14418 |
8444-8447 |
IN |
denotes |
due |
T14417 |
8437-8443 |
RB |
denotes |
likely |
T14416 |
8432-8436 |
RBS |
denotes |
most |
T14415 |
8417-8418 |
HYPH |
denotes |
- |
T14414 |
8418-8422 |
NN |
denotes |
cell |
T14413 |
8416-8417 |
CD |
denotes |
8 |
T14412 |
8423-8428 |
NN |
denotes |
stage |
T14411 |
8412-8415 |
DT |
denotes |
the |
T14410 |
8409-8411 |
IN |
denotes |
to |
T14409 |
8399-8400 |
SYM |
denotes |
- |
T14408 |
8398-8399 |
HYPH |
denotes |
/ |
T14407 |
8397-8398 |
SYM |
denotes |
- |
T14406 |
8392-8397 |
NN |
denotes |
Capn2 |
T14405 |
8388-8391 |
JJ |
denotes |
few |
T14404 |
8401-8408 |
NNS |
denotes |
embryos |
T14403 |
8386-8387 |
DT |
denotes |
a |
T14402 |
8383-8385 |
IN |
denotes |
of |
T14401 |
8374-8382 |
NN |
denotes |
survival |
T14400 |
8370-8373 |
DT |
denotes |
the |
T14399 |
8368-8370 |
, |
denotes |
, |
T14398 |
8359-8368 |
NN |
denotes |
viability |
T14397 |
8354-8358 |
NN |
denotes |
cell |
T14396 |
8351-8353 |
IN |
denotes |
of |
T14395 |
8344-8350 |
NN |
denotes |
aspect |
T14394 |
8339-8343 |
DT |
denotes |
some |
T14376 |
8267-8271 |
IN |
denotes |
into |
T14375 |
8262-8266 |
VBZ |
denotes |
fits |
T14374 |
8247-8252 |
NN |
denotes |
Capn2 |
T14373 |
8253-8261 |
NN |
denotes |
knockout |
T14372 |
8243-8246 |
DT |
denotes |
The |
T14371 |
8242-8292 |
sentence |
denotes |
The Capn2 knockout fits into the latter category. |
T14370 |
8241-8242 |
. |
denotes |
. |
T14369 |
8230-8232 |
VB |
denotes |
be |
T14368 |
8226-8229 |
RB |
denotes |
not |
T14367 |
8220-8225 |
MD |
denotes |
could |
T14366 |
8233-8241 |
VBN |
denotes |
isolated |
T14365 |
8211-8213 |
NN |
denotes |
ES |
T14364 |
8204-8210 |
NN |
denotes |
mutant |
T14363 |
8214-8219 |
NNS |
denotes |
cells |
T14362 |
8193-8203 |
JJ |
denotes |
homozygous |
T14361 |
8189-8192 |
CC |
denotes |
and |
T14360 |
8175-8179 |
RB |
denotes |
ever |
T14359 |
8171-8174 |
VBP |
denotes |
are |
T14358 |
8158-8162 |
JJ |
denotes |
null |
T14357 |
8154-8157 |
JJ |
denotes |
few |
T14356 |
8152-8153 |
DT |
denotes |
a |
T14355 |
8163-8170 |
NNS |
denotes |
embryos |
T14354 |
8147-8151 |
RB |
denotes |
only |
T14353 |
8145-8147 |
, |
denotes |
, |
T14352 |
8136-8145 |
NNS |
denotes |
instances |
T14351 |
8130-8135 |
DT |
denotes |
these |
T14350 |
8180-8188 |
VBN |
denotes |
observed |
T14349 |
8127-8129 |
IN |
denotes |
In |
T14348 |
8126-8242 |
sentence |
denotes |
In these instances, only a few null embryos are ever observed and homozygous mutant ES cells could not be isolated. |
T14347 |
8125-8126 |
. |
denotes |
. |
T14346 |
8124-8125 |
-RRB- |
denotes |
] |
T14345 |
8122-8124 |
CD |
denotes |
43 |
T14344 |
8121-8122 |
SYM |
denotes |
- |
T14343 |
8118-8119 |
, |
denotes |
, |
T14342 |
8117-8118 |
CD |
denotes |
3 |
T14341 |
8119-8121 |
CD |
denotes |
39 |
T14340 |
8116-8117 |
-LRB- |
denotes |
[ |
T14339 |
8103-8108 |
JJ |
denotes |
early |
T14338 |
8109-8115 |
NN |
denotes |
embryo |
T14337 |
8099-8102 |
DT |
denotes |
the |
T14336 |
8096-8098 |
IN |
denotes |
in |
T14335 |
8090-8095 |
NNS |
denotes |
cells |
T14334 |
8087-8089 |
IN |
denotes |
of |
T14333 |
8077-8086 |
NN |
denotes |
viability |
T14332 |
8073-8076 |
DT |
denotes |
the |
T14331 |
8065-8072 |
RB |
denotes |
fatally |
T14330 |
8054-8064 |
VB |
denotes |
compromise |
T14329 |
8051-8053 |
TO |
denotes |
to |
T14328 |
8040-8042 |
VBZ |
denotes |
is |
T14327 |
8024-8028 |
NN |
denotes |
gene |
T14326 |
8029-8039 |
NN |
denotes |
disruption |
T14325 |
8020-8023 |
DT |
denotes |
the |
T14324 |
8018-8020 |
, |
denotes |
, |
T14323 |
8011-8018 |
RB |
denotes |
however |
T14322 |
8009-8011 |
, |
denotes |
, |
T14321 |
8004-8009 |
NNS |
denotes |
cases |
T14320 |
7998-8003 |
JJ |
denotes |
other |
T14319 |
8043-8050 |
VBN |
denotes |
thought |
T14318 |
7995-7997 |
IN |
denotes |
In |
T14317 |
7994-8126 |
sentence |
denotes |
In other cases, however, the gene disruption is thought to compromise fatally the viability of cells in the early embryo [3,39-43]. |
T14316 |
7993-7994 |
. |
denotes |
. |
T14315 |
7980-7987 |
RB |
denotes |
usually |
T14314 |
7977-7979 |
VBZ |
denotes |
is |
T14313 |
7967-7976 |
NNS |
denotes |
genotypes |
T14312 |
7957-7966 |
JJ |
denotes |
embryonic |
T14311 |
7954-7956 |
IN |
denotes |
of |
T14310 |
7988-7993 |
VBN |
denotes |
noted |
T14309 |
7931-7940 |
JJ |
denotes |
Mendelian |
T14308 |
7941-7953 |
NN |
denotes |
distribution |
T14307 |
7929-7930 |
DT |
denotes |
a |
T14306 |
7925-7928 |
CC |
denotes |
and |
T14305 |
7923-7925 |
, |
denotes |
, |
T14304 |
7911-7917 |
NN |
denotes |
morula |
T14303 |
7918-7923 |
NN |
denotes |
stage |
T14302 |
7907-7910 |
DT |
denotes |
the |
T14301 |
7900-7906 |
IN |
denotes |
beyond |
T14300 |
7886-7891 |
RB |
denotes |
often |
T14299 |
7881-7885 |
NN |
denotes |
type |
T14298 |
7876-7880 |
DT |
denotes |
this |
T14297 |
7873-7875 |
IN |
denotes |
of |
T14296 |
7892-7899 |
VBP |
denotes |
survive |
T14295 |
7865-7872 |
NNS |
denotes |
embryos |
T14294 |
7860-7864 |
JJ |
denotes |
Null |
T14293 |
7859-7994 |
sentence |
denotes |
Null embryos of this type often survive beyond the morula stage, and a Mendelian distribution of embryonic genotypes is usually noted. |
T14292 |
7858-7859 |
. |
denotes |
. |
T14291 |
7857-7858 |
-RRB- |
denotes |
] |
T14290 |
7855-7857 |
CD |
denotes |
38 |
T14289 |
7854-7855 |
-LRB- |
denotes |
[ |
T14288 |
7838-7853 |
RB |
denotes |
morphologically |
T14287 |
7826-7828 |
VB |
denotes |
be |
T14286 |
7820-7825 |
RB |
denotes |
often |
T14285 |
7816-7819 |
MD |
denotes |
can |
T14284 |
7829-7837 |
VBN |
denotes |
observed |
T14283 |
7810-7815 |
WDT |
denotes |
which |
T14282 |
7786-7801 |
NN |
denotes |
differentiation |
T14281 |
7776-7785 |
JJ |
denotes |
embryonic |
T14280 |
7802-7809 |
NN |
denotes |
program |
T14279 |
7772-7775 |
DT |
denotes |
the |
T14278 |
7769-7771 |
IN |
denotes |
in |
T14277 |
7756-7759 |
VBP |
denotes |
are |
T14276 |
7748-7755 |
NNS |
denotes |
defects |
T14275 |
7746-7748 |
, |
denotes |
, |
T14274 |
7741-7746 |
NNS |
denotes |
cases |
T14273 |
7736-7740 |
DT |
denotes |
some |
T14272 |
7760-7768 |
VBN |
denotes |
incurred |
T14271 |
7733-7735 |
IN |
denotes |
In |
T14270 |
7732-7859 |
sentence |
denotes |
In some cases, defects are incurred in the embryonic differentiation program which can often be observed morphologically [38]. |
T14269 |
7731-7732 |
. |
denotes |
. |
T14268 |
7717-7724 |
JJ |
denotes |
general |
T14267 |
7725-7731 |
NNS |
denotes |
causes |
T14266 |
7713-7716 |
CD |
denotes |
two |
T14265 |
7710-7712 |
IN |
denotes |
to |
T14264 |
7696-7698 |
VB |
denotes |
be |
T14263 |
7686-7695 |
RB |
denotes |
typically |
T14262 |
7682-7685 |
MD |
denotes |
can |
T14261 |
7672-7681 |
NNS |
denotes |
knockouts |
T14260 |
7667-7671 |
NN |
denotes |
gene |
T14259 |
7662-7666 |
IN |
denotes |
from |
T14258 |
7652-7661 |
VBG |
denotes |
resulting |
T14257 |
7699-7709 |
VBN |
denotes |
attributed |
T14256 |
7642-7651 |
NN |
denotes |
lethality |
T14255 |
7626-7641 |
NN |
denotes |
Preimplantation |
T14254 |
7625-7732 |
sentence |
denotes |
Preimplantation lethality resulting from gene knockouts can typically be attributed to two general causes. |
T14253 |
7624-7625 |
. |
denotes |
. |
T14252 |
7615-7624 |
VBG |
denotes |
revealing |
T14251 |
7608-7614 |
PRP |
denotes |
itself |
T14250 |
7605-7607 |
IN |
denotes |
in |
T14249 |
7600-7601 |
SYM |
denotes |
- |
T14248 |
7599-7600 |
HYPH |
denotes |
/ |
T14247 |
7598-7599 |
SYM |
denotes |
- |
T14246 |
7593-7598 |
NN |
denotes |
Capn2 |
T14245 |
7590-7592 |
VB |
denotes |
be |
T14244 |
7587-7589 |
TO |
denotes |
to |
T14243 |
7555-7571 |
JJ |
denotes |
pre-implantation |
T14242 |
7545-7554 |
VBN |
denotes |
genotyped |
T14241 |
7532-7544 |
RB |
denotes |
successfully |
T14240 |
7526-7528 |
IN |
denotes |
of |
T14239 |
7522-7525 |
IN |
denotes |
out |
T14238 |
7529-7531 |
CD |
denotes |
90 |
T14237 |
7518-7521 |
CD |
denotes |
two |
T14236 |
7572-7579 |
NNS |
denotes |
embryos |
T14235 |
7513-7517 |
RB |
denotes |
only |
T14234 |
7580-7586 |
VBD |
denotes |
proved |
T14233 |
7508-7512 |
IN |
denotes |
that |
T14232 |
7503-7507 |
NN |
denotes |
fact |
T14231 |
7499-7502 |
DT |
denotes |
the |
T14230 |
7497-7499 |
, |
denotes |
, |
T14229 |
7602-7604 |
VBZ |
denotes |
is |
T14228 |
7490-7497 |
RB |
denotes |
However |
T14227 |
7489-7625 |
sentence |
denotes |
However, the fact that only two out of 90 successfully genotyped pre-implantation embryos proved to be Capn2-/- is in itself revealing. |
T14226 |
7488-7489 |
. |
denotes |
. |
T14225 |
7467-7480 |
JJ |
denotes |
morphological |
T14224 |
7459-7466 |
JJ |
denotes |
obvious |
T14223 |
7481-7488 |
NNS |
denotes |
defects |
T14222 |
7455-7458 |
DT |
denotes |
any |
T14221 |
7443-7446 |
RB |
denotes |
not |
T14220 |
7439-7442 |
VBD |
denotes |
did |
T14219 |
7427-7428 |
HYPH |
denotes |
- |
T14218 |
7428-7432 |
NN |
denotes |
cell |
T14217 |
7426-7427 |
CD |
denotes |
8 |
T14216 |
7433-7438 |
NN |
denotes |
stage |
T14215 |
7422-7425 |
DT |
denotes |
the |
T14214 |
7419-7421 |
IN |
denotes |
at |
T14213 |
7408-7418 |
VBN |
denotes |
identified |
T14212 |
7447-7454 |
VB |
denotes |
present |
T14211 |
7395-7399 |
JJ |
denotes |
null |
T14210 |
7384-7394 |
JJ |
denotes |
homozygous |
T14209 |
7380-7383 |
CD |
denotes |
two |
T14208 |
7400-7407 |
NNS |
denotes |
embryos |
T14207 |
7376-7379 |
DT |
denotes |
The |
T14206 |
7375-7489 |
sentence |
denotes |
The two homozygous null embryos identified at the 8-cell stage did not present any obvious morphological defects. |
T14205 |
7374-7375 |
. |
denotes |
. |
T14204 |
7360-7364 |
VBN |
denotes |
been |
T14203 |
7356-7359 |
RB |
denotes |
yet |
T14202 |
7352-7355 |
RB |
denotes |
not |
T14201 |
7348-7351 |
VBZ |
denotes |
has |
T14200 |
7338-7339 |
SYM |
denotes |
- |
T14199 |
7337-7338 |
HYPH |
denotes |
/ |
T14198 |
7336-7337 |
SYM |
denotes |
- |
T14197 |
7340-7347 |
NNS |
denotes |
embryos |
T14196 |
7331-7336 |
NN |
denotes |
Capn2 |
T14669 |
9626-9635 |
VBN |
denotes |
increased |
T14668 |
9623-9625 |
IN |
denotes |
to |
T14667 |
9610-9616 |
NN |
denotes |
oocyte |
T14666 |
9606-9609 |
DT |
denotes |
the |
T14665 |
9603-9605 |
IN |
denotes |
to |
T14664 |
9617-9622 |
VBZ |
denotes |
leads |
T14663 |
9595-9602 |
NN |
denotes |
binding |
T14662 |
9589-9594 |
NN |
denotes |
Sperm |
T14661 |
9588-9698 |
sentence |
denotes |
Sperm binding to the oocyte leads to increased cytoplasmic calcium which triggers the acrosome reaction [46]. |
T14660 |
9587-9588 |
. |
denotes |
. |
T14659 |
9586-9587 |
-RRB- |
denotes |
] |
T14658 |
9585-9586 |
CD |
denotes |
3 |
T14657 |
9584-9585 |
-LRB- |
denotes |
[ |
T14656 |
9573-9583 |
NNS |
denotes |
substrates |
T14655 |
9565-9572 |
NN |
denotes |
calpain |
T14654 |
9562-9564 |
VB |
denotes |
be |
T14653 |
9559-9561 |
TO |
denotes |
to |
T14652 |
9547-9550 |
VBP |
denotes |
are |
T14651 |
9539-9546 |
NN |
denotes |
p27kip1 |
T14650 |
9535-9538 |
CC |
denotes |
and |
T14649 |
9533-9535 |
, |
denotes |
, |
T14648 |
9531-9533 |
NN |
denotes |
D1 |
T14647 |
9524-9530 |
NN |
denotes |
cyclin |
T14646 |
9522-9524 |
, |
denotes |
, |
T14645 |
9518-9522 |
NN |
denotes |
p107 |
T14644 |
9516-9518 |
, |
denotes |
, |
T14643 |
9513-9516 |
NN |
denotes |
p53 |
T14642 |
9510-9512 |
IN |
denotes |
as |
T14641 |
9505-9509 |
JJ |
denotes |
such |
T14640 |
9503-9505 |
, |
denotes |
, |
T14639 |
9492-9503 |
NN |
denotes |
progression |
T14638 |
9486-9491 |
NN |
denotes |
cycle |
T14637 |
9481-9485 |
NN |
denotes |
cell |
T14636 |
9478-9480 |
IN |
denotes |
in |
T14635 |
9466-9468 |
VB |
denotes |
be |
T14634 |
9469-9477 |
VBN |
denotes |
involved |
T14633 |
9463-9465 |
TO |
denotes |
to |
T14632 |
9457-9462 |
VBN |
denotes |
known |
T14631 |
9448-9456 |
NN |
denotes |
proteins |
T14630 |
9444-9447 |
DT |
denotes |
the |
T14629 |
9441-9443 |
IN |
denotes |
of |
T14628 |
9436-9440 |
DT |
denotes |
some |
T14627 |
9434-9436 |
, |
denotes |
, |
T14626 |
9551-9558 |
VBN |
denotes |
reputed |
T14625 |
9423-9434 |
RB |
denotes |
Furthermore |
T14624 |
9422-9588 |
sentence |
denotes |
Furthermore, some of the proteins known to be involved in cell cycle progression, such as p53, p107, cyclin D1, and p27kip1 are reputed to be calpain substrates [3]. |
T14623 |
9421-9422 |
. |
denotes |
. |
T14622 |
9420-9421 |
-RRB- |
denotes |
] |
T14621 |
9419-9420 |
CD |
denotes |
6 |
T14620 |
9418-9419 |
-LRB- |
denotes |
[ |
T14619 |
9407-9411 |
NN |
denotes |
cell |
T14618 |
9412-9417 |
NN |
denotes |
cycle |
T14617 |
9403-9406 |
DT |
denotes |
the |
T14616 |
9400-9402 |
IN |
denotes |
of |
T14615 |
9391-9393 |
NN |
denotes |
G1 |
T14614 |
9394-9399 |
NN |
denotes |
stage |
T14613 |
9387-9390 |
DT |
denotes |
the |
T14612 |
9379-9386 |
IN |
denotes |
through |
T14611 |
9367-9378 |
NN |
denotes |
progression |
T14610 |
9362-9366 |
IN |
denotes |
with |
T14195 |
7328-7330 |
IN |
denotes |
in |
T14194 |
7302-7317 |
NN |
denotes |
preimplantation |
T14193 |
7318-7327 |
NN |
denotes |
lethality |
T14192 |
7298-7301 |
DT |
denotes |
the |
T14191 |
7295-7297 |
IN |
denotes |
of |
T14190 |
7365-7374 |
VBN |
denotes |
clarified |
T14189 |
7278-7288 |
VBG |
denotes |
underlying |
T14188 |
7289-7294 |
NN |
denotes |
cause |
T14187 |
7274-7277 |
DT |
denotes |
The |
T14186 |
7273-7375 |
sentence |
denotes |
The underlying cause of the preimplantation lethality in Capn2-/- embryos has not yet been clarified. |
T14185 |
7272-7273 |
. |
denotes |
. |
T14184 |
7264-7272 |
JJ |
denotes |
distinct |
T14183 |
7251-7252 |
HYPH |
denotes |
- |
T14182 |
7252-7259 |
NN |
denotes |
calpain |
T14181 |
7250-7251 |
NN |
denotes |
m |
T14180 |
7246-7249 |
CC |
denotes |
and |
T14179 |
7237-7238 |
HYPH |
denotes |
- |
T14178 |
7238-7245 |
NN |
denotes |
calpain |
T14177 |
7236-7237 |
NN |
denotes |
μ |
T14176 |
7233-7235 |
IN |
denotes |
of |
T14175 |
7218-7222 |
DT |
denotes |
some |
T14174 |
7223-7232 |
NNS |
denotes |
functions |
T14173 |
7212-7217 |
RBS |
denotes |
least |
T14172 |
7209-7211 |
RB |
denotes |
at |
T14171 |
7260-7263 |
VBP |
denotes |
are |
T14170 |
7204-7208 |
IN |
denotes |
that |
T14169 |
7196-7203 |
VBZ |
denotes |
follows |
T14168 |
7193-7195 |
PRP |
denotes |
It |
T14167 |
7192-7273 |
sentence |
denotes |
It follows that at least some functions of μ-calpain and m-calpain are distinct. |
T14166 |
7191-7192 |
. |
denotes |
. |
T14165 |
7180-7181 |
HYPH |
denotes |
- |
T14164 |
7181-7185 |
NN |
denotes |
cell |
T14163 |
7179-7180 |
CD |
denotes |
8 |
T14162 |
7186-7191 |
NN |
denotes |
stage |
T14161 |
7175-7178 |
DT |
denotes |
the |
T14040 |
6559-6566 |
NNS |
denotes |
amounts |
T14039 |
6544-6549 |
PRP$ |
denotes |
their |
T14038 |
6541-6543 |
IN |
denotes |
of |
T14037 |
6531-6540 |
NNS |
denotes |
estimates |
T14036 |
6522-6530 |
JJ |
denotes |
reliable |
T14035 |
6510-6513 |
RB |
denotes |
not |
T14034 |
6507-6509 |
VBP |
denotes |
do |
T14033 |
6505-6507 |
, |
denotes |
, |
T14032 |
6484-6493 |
JJ |
denotes |
available |
T14031 |
6494-6505 |
NN |
denotes |
methodology |
T14030 |
6480-6483 |
DT |
denotes |
the |
T14029 |
6477-6479 |
IN |
denotes |
in |
T14028 |
6466-6476 |
NNS |
denotes |
weaknesses |
T14027 |
6463-6465 |
IN |
denotes |
to |
T14026 |
6457-6462 |
VBG |
denotes |
owing |
T14025 |
6455-6457 |
, |
denotes |
, |
T14024 |
6441-6450 |
VBN |
denotes |
published |
T14023 |
6451-6455 |
NNS |
denotes |
data |
T14022 |
6437-6440 |
DT |
denotes |
the |
T14021 |
6514-6521 |
VB |
denotes |
provide |
T14020 |
6428-6436 |
IN |
denotes |
although |
T14019 |
6426-6428 |
, |
denotes |
, |
T14018 |
6411-6420 |
JJ |
denotes |
mammalian |
T14017 |
6421-6426 |
NNS |
denotes |
cells |
T14016 |
6406-6410 |
JJS |
denotes |
most |
T14015 |
6403-6405 |
IN |
denotes |
in |
T14014 |
6395-6402 |
JJ |
denotes |
present |
T14013 |
6391-6394 |
VBP |
denotes |
are |
T14012 |
6371-6372 |
HYPH |
denotes |
- |
T14011 |
6370-6371 |
NN |
denotes |
m |
T14010 |
6366-6369 |
CC |
denotes |
and |
T14009 |
6364-6365 |
HYPH |
denotes |
- |
T14008 |
6372-6379 |
NN |
denotes |
calpain |
T14007 |
6363-6364 |
NN |
denotes |
μ |
T14006 |
6380-6390 |
NNS |
denotes |
activities |
T14005 |
6358-6362 |
CC |
denotes |
Both |
T14004 |
6357-6567 |
sentence |
denotes |
Both μ- and m-calpain activities are present in most mammalian cells, although the published data, owing to weaknesses in the available methodology, do not provide reliable estimates of their relative amounts. |
T14003 |
6356-6357 |
. |
denotes |
. |
T14002 |
6346-6356 |
NN |
denotes |
activation |
T14001 |
6337-6345 |
NN |
denotes |
platelet |
T14000 |
6334-6336 |
IN |
denotes |
in |
T13999 |
6325-6333 |
VBN |
denotes |
involved |
T13998 |
6316-6324 |
NN |
denotes |
proteins |
T13997 |
6308-6315 |
JJ |
denotes |
certain |
T13996 |
6305-6307 |
IN |
denotes |
of |
T13995 |
6280-6288 |
NN |
denotes |
tyrosine |
T13994 |
6289-6304 |
NN |
denotes |
phosphorylation |
T13993 |
6276-6279 |
DT |
denotes |
the |
T13992 |
6273-6275 |
IN |
denotes |
of |
T13991 |
6267-6272 |
NN |
denotes |
level |
T13990 |
6263-6266 |
DT |
denotes |
the |
T13989 |
6260-6262 |
IN |
denotes |
at |
T13988 |
6251-6259 |
RB |
denotes |
possibly |
T13987 |
6249-6251 |
, |
denotes |
, |
T13986 |
6241-6249 |
NN |
denotes |
function |
T13985 |
6232-6240 |
NN |
denotes |
platelet |
T13984 |
6229-6231 |
IN |
denotes |
in |
T13983 |
6217-6228 |
NN |
denotes |
disturbance |
T13982 |
6215-6216 |
DT |
denotes |
a |
T13981 |
6195-6205 |
NN |
denotes |
deficiency |
T13980 |
6189-6194 |
NN |
denotes |
Capn1 |
T13979 |
6186-6188 |
IN |
denotes |
of |
T13978 |
6179-6185 |
NN |
denotes |
result |
T13977 |
6177-6178 |
DT |
denotes |
a |
T13976 |
6174-6176 |
IN |
denotes |
as |
T13975 |
6165-6173 |
VBN |
denotes |
observed |
T13974 |
6206-6214 |
VBD |
denotes |
involved |
T13973 |
6145-6154 |
JJ |
denotes |
principal |
T13972 |
6155-6164 |
NN |
denotes |
phenotype |
T13971 |
6141-6144 |
DT |
denotes |
The |
T13970 |
6140-6357 |
sentence |
denotes |
The principal phenotype observed as a result of Capn1 deficiency involved a disturbance in platelet function, possibly at the level of the tyrosine phosphorylation of certain proteins involved in platelet activation. |
T13969 |
6139-6140 |
. |
denotes |
. |
T13968 |
6138-6139 |
-RRB- |
denotes |
] |
T13967 |
6136-6138 |
CD |
denotes |
33 |
T13966 |
6135-6136 |
-LRB- |
denotes |
[ |
T13965 |
6130-6134 |
NNS |
denotes |
mice |
T13964 |
6127-6129 |
IN |
denotes |
of |
T13963 |
6117-6126 |
NN |
denotes |
viability |
T13962 |
6113-6116 |
DT |
denotes |
the |
T13961 |
6102-6105 |
RB |
denotes |
not |
T13960 |
6098-6101 |
VBD |
denotes |
did |
T13959 |
6096-6098 |
, |
denotes |
, |
T13958 |
6091-6096 |
NN |
denotes |
Capn1 |
T13957 |
6089-6091 |
, |
denotes |
, |
T13956 |
6077-6084 |
NN |
denotes |
subunit |
T13955 |
6071-6076 |
JJ |
denotes |
large |
T13954 |
6062-6063 |
HYPH |
denotes |
- |
T13953 |
6063-6070 |
NN |
denotes |
calpain |
T13952 |
6061-6062 |
NN |
denotes |
μ |
T13951 |
6085-6089 |
NN |
denotes |
gene |
T13950 |
6057-6060 |
DT |
denotes |
the |
T13949 |
6054-6056 |
IN |
denotes |
of |
T13948 |
6043-6053 |
NN |
denotes |
disruption |
T13947 |
6032-6042 |
JJ |
denotes |
homozygous |
T13946 |
6030-6032 |
, |
denotes |
, |
T13945 |
6018-6030 |
NN |
denotes |
implantation |
T13944 |
6015-6017 |
IN |
denotes |
to |
T13943 |
6009-6014 |
JJ |
denotes |
prior |
T13942 |
5990-5996 |
JJ |
denotes |
murine |
T13941 |
5997-6004 |
NNS |
denotes |
embryos |
T13940 |
5985-5989 |
JJ |
denotes |
null |
T13939 |
5979-5984 |
NN |
denotes |
Capn2 |
T13938 |
6005-6008 |
VBP |
denotes |
die |
T13937 |
5971-5978 |
IN |
denotes |
whereas |
T13936 |
5969-5971 |
, |
denotes |
, |
T13935 |
6106-6112 |
VB |
denotes |
affect |
T13934 |
5964-5969 |
VBN |
denotes |
noted |
T13933 |
5961-5963 |
IN |
denotes |
As |
T13932 |
5960-6140 |
sentence |
denotes |
As noted, whereas Capn2 null murine embryos die prior to implantation, homozygous disruption of the μ-calpain large subunit gene, Capn1, did not affect the viability of mice [33]. |
T13931 |
5959-5960 |
. |
denotes |
. |
T13930 |
5946-5959 |
NN |
denotes |
embryogenesis |
T13929 |
5939-5945 |
IN |
denotes |
during |
T13928 |
5933-5938 |
RBS |
denotes |
least |
T13927 |
5930-5932 |
RB |
denotes |
at |
T13926 |
5928-5930 |
, |
denotes |
, |
T13925 |
5909-5922 |
JJ |
denotes |
physiological |
T13924 |
5900-5908 |
JJ |
denotes |
distinct |
T13923 |
5923-5928 |
NNS |
denotes |
roles |
T13922 |
5895-5899 |
DT |
denotes |
some |
T13921 |
5881-5882 |
HYPH |
denotes |
- |
T13920 |
5880-5881 |
NN |
denotes |
m |
T13919 |
5876-5879 |
CC |
denotes |
and |
T13918 |
5874-5875 |
HYPH |
denotes |
- |
T13917 |
5882-5889 |
NN |
denotes |
calpain |
T13916 |
5873-5874 |
NN |
denotes |
μ |
T13915 |
5890-5894 |
VBP |
denotes |
have |
T13914 |
5868-5872 |
IN |
denotes |
that |
T13913 |
5866-5868 |
, |
denotes |
, |
T13912 |
5862-5866 |
NNS |
denotes |
mice |
T13911 |
5859-5861 |
IN |
denotes |
in |
T13910 |
5854-5858 |
VBN |
denotes |
done |
T13909 |
5839-5848 |
NN |
denotes |
targeting |
T13908 |
5834-5838 |
NN |
denotes |
gene |
T13907 |
5849-5853 |
NN |
denotes |
work |
T13906 |
5830-5833 |
DT |
denotes |
the |
T13905 |
5825-5829 |
IN |
denotes |
from |
T13904 |
5823-5825 |
, |
denotes |
, |
T13903 |
5818-5823 |
JJ |
denotes |
clear |
T13902 |
5814-5817 |
RB |
denotes |
now |
T13901 |
5811-5813 |
VBZ |
denotes |
is |
T13900 |
5808-5810 |
PRP |
denotes |
It |
T13899 |
5807-5960 |
sentence |
denotes |
It is now clear, from the gene targeting work done in mice, that μ- and m-calpain have some distinct physiological roles, at least during embryogenesis. |
T13898 |
5806-5807 |
. |
denotes |
. |
T13897 |
5801-5806 |
NNS |
denotes |
cells |
T13896 |
5794-5800 |
IN |
denotes |
inside |
T13895 |
5769-5783 |
RB |
denotes |
differentially |
T13894 |
5765-5768 |
VBP |
denotes |
are |
T13893 |
5752-5755 |
CD |
denotes |
two |
T13892 |
5756-5764 |
NNS |
denotes |
isoforms |
T13891 |
5748-5751 |
DT |
denotes |
the |
T13890 |
5784-5793 |
VBN |
denotes |
regulated |
T13889 |
5743-5747 |
IN |
denotes |
that |
T13888 |
5734-5742 |
JJ |
denotes |
possible |
T13887 |
5728-5730 |
PRP |
denotes |
it |
T13886 |
5726-5728 |
, |
denotes |
, |
T13885 |
5716-5726 |
NN |
denotes |
activation |
T13884 |
5710-5715 |
FW |
denotes |
vitro |
T13883 |
5707-5709 |
FW |
denotes |
in |
T13882 |
5703-5706 |
IN |
denotes |
for |
T13881 |
5701-5702 |
SYM |
denotes |
+ |
T13880 |
5698-5701 |
NN |
denotes |
Ca2 |
T13879 |
5695-5697 |
IN |
denotes |
of |
T13878 |
5687-5694 |
NNS |
denotes |
amounts |
T13877 |
5677-5686 |
JJ |
denotes |
different |
T13876 |
5664-5668 |
PRP |
denotes |
they |
T13875 |
5669-5676 |
VBP |
denotes |
require |
T13874 |
5658-5663 |
IN |
denotes |
since |
T13873 |
5656-5658 |
, |
denotes |
, |
T13872 |
5646-5651 |
JJ |
denotes |
other |
T13871 |
5652-5656 |
NN |
denotes |
hand |
T13870 |
5642-5645 |
DT |
denotes |
the |
T13869 |
5731-5733 |
VBZ |
denotes |
is |
T13868 |
5639-5641 |
IN |
denotes |
On |
T13867 |
5638-5807 |
sentence |
denotes |
On the other hand, since they require different amounts of Ca2+ for in vitro activation, it is possible that the two isoforms are differentially regulated inside cells. |
T13866 |
5637-5638 |
. |
denotes |
. |
T13865 |
5633-5637 |
FW |
denotes |
vivo |
T13864 |
5630-5632 |
FW |
denotes |
in |
T13863 |
5615-5619 |
JJ |
denotes |
same |
T13862 |
5620-5629 |
NNS |
denotes |
functions |
T13861 |
5611-5614 |
DT |
denotes |
the |
T13860 |
5607-5610 |
RP |
denotes |
out |
T14393 |
8335-8338 |
IN |
denotes |
for |
T14392 |
8325-8334 |
JJ |
denotes |
essential |
T14391 |
8313-8314 |
HYPH |
denotes |
- |
T14390 |
8314-8321 |
NN |
denotes |
calpain |
T14389 |
8312-8313 |
NN |
denotes |
m |
T14388 |
8322-8324 |
VBZ |
denotes |
is |
T14387 |
8307-8311 |
IN |
denotes |
that |
T14386 |
8302-8306 |
JJ |
denotes |
true |
T14385 |
8429-8431 |
VBZ |
denotes |
is |
T14384 |
8296-8298 |
PRP |
denotes |
it |
T14383 |
8299-8301 |
VBZ |
denotes |
is |
T14382 |
8293-8295 |
IN |
denotes |
If |
T14381 |
8292-8541 |
sentence |
denotes |
If it is true that m-calpain is essential for some aspect of cell viability, the survival of a few Capn2-/- embryos to the 8-cell stage is most likely due to the persistence of some maternal m-calpain mRNA and/or protein through 2–3 cell divisions. |
T14380 |
8291-8292 |
. |
denotes |
. |
T14379 |
8276-8282 |
JJ |
denotes |
latter |
T14378 |
8283-8291 |
NN |
denotes |
category |
T14377 |
8272-8275 |
DT |
denotes |
the |
T13859 |
5601-5606 |
VB |
denotes |
carry |
T13858 |
5598-5600 |
TO |
denotes |
to |
T13857 |
5588-5597 |
NN |
denotes |
potential |
T13856 |
5584-5587 |
DT |
denotes |
the |
T13855 |
5574-5578 |
PRP |
denotes |
they |
T13854 |
5579-5583 |
VBP |
denotes |
have |
T13853 |
5569-5573 |
IN |
denotes |
that |
T13852 |
5558-5568 |
VBG |
denotes |
suggesting |
T13851 |
5556-5558 |
, |
denotes |
, |
T13850 |
5551-5556 |
FW |
denotes |
vitro |
T13849 |
5548-5550 |
FW |
denotes |
in |
T13848 |
5537-5547 |
NNS |
denotes |
substrates |
T13847 |
5534-5536 |
IN |
denotes |
of |
T13846 |
5525-5529 |
JJ |
denotes |
same |
T13845 |
5530-5533 |
NN |
denotes |
set |
T13844 |
5521-5524 |
DT |
denotes |
the |
T13843 |
5509-5520 |
RB |
denotes |
essentially |
T13842 |
5497-5501 |
PRP |
denotes |
they |
T13841 |
5495-5497 |
, |
denotes |
, |
T13840 |
5502-5508 |
VBP |
denotes |
cleave |
T13839 |
5488-5495 |
RB |
denotes |
Notably |
T13838 |
5487-5638 |
sentence |
denotes |
Notably, they cleave essentially the same set of substrates in vitro, suggesting that they have the potential to carry out the same functions in vivo. |
T13837 |
5486-5487 |
. |
denotes |
. |
T13836 |
5474-5486 |
NN |
denotes |
biochemistry |
T13835 |
5470-5473 |
CC |
denotes |
and |
T13834 |
5460-5469 |
NN |
denotes |
structure |
T13833 |
5454-5459 |
PRP$ |
denotes |
their |
T13832 |
5451-5453 |
IN |
denotes |
in |
T13831 |
5443-5450 |
JJ |
denotes |
similar |
T13830 |
5438-5442 |
RB |
denotes |
very |
T13829 |
5434-5437 |
VBP |
denotes |
are |
T13828 |
5430-5433 |
CC |
denotes |
and |
T13827 |
5412-5420 |
NN |
denotes |
sequence |
T13826 |
5421-5429 |
NN |
denotes |
identity |
T13825 |
5410-5411 |
NN |
denotes |
% |
T13745 |
5002-5008 |
NN |
denotes |
timing |
T13744 |
4988-4991 |
DT |
denotes |
The |
T13743 |
4987-5239 |
sentence |
denotes |
The different timing of lethality in the Capn4 knockouts might also be a consequence of the different genetic backgrounds of the two transgenic lines, which has been observed to influence the phenotype of transgenic mice on a number of occasions [37]. |
T13742 |
4986-4987 |
. |
denotes |
. |
T13741 |
4985-4986 |
-RRB- |
denotes |
] |
T13740 |
4983-4985 |
CD |
denotes |
36 |
T13739 |
4982-4983 |
-LRB- |
denotes |
[ |
T13738 |
4976-4981 |
NNS |
denotes |
cells |
T13737 |
4966-4975 |
JJ |
denotes |
mammalian |
T13736 |
4963-4965 |
IN |
denotes |
in |
T13735 |
4960-4962 |
CC |
denotes |
or |
T13734 |
4955-4959 |
NNP |
denotes |
coli |
T13733 |
4952-4954 |
NNP |
denotes |
E. |
T13732 |
4949-4951 |
IN |
denotes |
in |
T13731 |
4942-4948 |
CC |
denotes |
either |
T13730 |
4936-4941 |
RB |
denotes |
alone |
T13729 |
4921-4925 |
VBD |
denotes |
were |
T13728 |
4906-4911 |
JJ |
denotes |
large |
T13727 |
4912-4920 |
NNS |
denotes |
subunits |
T13726 |
4898-4905 |
NN |
denotes |
calpain |
T13725 |
4926-4935 |
VBN |
denotes |
expressed |
T13724 |
4893-4897 |
WRB |
denotes |
when |
T13723 |
4887-4892 |
NNS |
denotes |
hands |
T13722 |
4883-4886 |
PRP$ |
denotes |
our |
T13721 |
4880-4882 |
IN |
denotes |
in |
T13720 |
4867-4870 |
VBD |
denotes |
was |
T13719 |
4871-4879 |
VBN |
denotes |
observed |
T13718 |
4850-4857 |
NN |
denotes |
calpain |
T13717 |
4858-4866 |
NN |
denotes |
activity |
T13716 |
4847-4849 |
DT |
denotes |
no |
T13715 |
4843-4846 |
CC |
denotes |
and |
T13714 |
4841-4843 |
, |
denotes |
, |
T13713 |
4830-4841 |
NN |
denotes |
possibility |
T13712 |
4825-4829 |
DT |
denotes |
that |
T13711 |
4817-4824 |
VB |
denotes |
exclude |
T13710 |
4814-4816 |
TO |
denotes |
to |
T13709 |
4793-4796 |
FW |
denotes |
al. |
T13708 |
4790-4792 |
FW |
denotes |
et |
T13707 |
4780-4789 |
NNP |
denotes |
Zimmerman |
T13706 |
4797-4805 |
NN |
denotes |
knockout |
T13705 |
4776-4779 |
DT |
denotes |
the |
T13704 |
4806-4813 |
VBZ |
denotes |
appears |
T13703 |
4767-4775 |
IN |
denotes |
although |
T13702 |
4765-4767 |
, |
denotes |
, |
T13701 |
4760-4765 |
NNS |
denotes |
cells |
T13700 |
4749-4759 |
JJ |
denotes |
eukaryotic |
T13699 |
4746-4748 |
IN |
denotes |
in |
T13698 |
4737-4745 |
NN |
denotes |
activity |
T13697 |
4732-4736 |
DT |
denotes |
some |
T13696 |
4718-4723 |
MD |
denotes |
might |
T13695 |
4712-4717 |
RB |
denotes |
alone |
T13694 |
4697-4702 |
JJ |
denotes |
large |
T13693 |
4703-4711 |
NNS |
denotes |
subunits |
T13692 |
4689-4696 |
NN |
denotes |
calpain |
T13691 |
4724-4731 |
VB |
denotes |
provide |
T13690 |
4684-4688 |
IN |
denotes |
that |
T13689 |
4669-4673 |
VBN |
denotes |
been |
T13688 |
4664-4668 |
RB |
denotes |
also |
T13687 |
4660-4663 |
VBZ |
denotes |
has |
T13686 |
4657-4659 |
PRP |
denotes |
It |
T13685 |
4655-4656 |
-RRB- |
denotes |
) |
T13684 |
4651-4655 |
NN |
denotes |
work |
T13683 |
4639-4650 |
JJ |
denotes |
unpublished |
T13682 |
4637-4639 |
, |
denotes |
, |
T13681 |
4633-4637 |
NNP |
denotes |
Elce |
T13680 |
4628-4632 |
NNP |
denotes |
J.S. |
T13679 |
4627-4628 |
-LRB- |
denotes |
( |
T13678 |
4613-4615 |
VB |
denotes |
be |
T13677 |
4616-4626 |
VBN |
denotes |
determined |
T13676 |
4610-4612 |
TO |
denotes |
to |
T13675 |
4606-4609 |
RB |
denotes |
yet |
T13674 |
4596-4601 |
NNS |
denotes |
cells |
T13673 |
4586-4595 |
JJ |
denotes |
mammalian |
T13672 |
4583-4585 |
IN |
denotes |
in |
T13671 |
4569-4582 |
NN |
denotes |
functionality |
T13670 |
4563-4568 |
PRP$ |
denotes |
their |
T13669 |
4602-4605 |
VBZ |
denotes |
has |
T13668 |
4554-4562 |
IN |
denotes |
although |
T13667 |
4552-4554 |
, |
denotes |
, |
T13666 |
4548-4552 |
NNP |
denotes |
coli |
T13665 |
4545-4547 |
NNP |
denotes |
E. |
T13664 |
4542-4544 |
IN |
denotes |
in |
T13663 |
4532-4541 |
VBN |
denotes |
expressed |
T13662 |
4527-4531 |
WRB |
denotes |
when |
T13661 |
4510-4517 |
NN |
denotes |
calpain |
T13660 |
4499-4509 |
JJ |
denotes |
detectable |
T13659 |
4518-4526 |
NN |
denotes |
activity |
T13658 |
4495-4498 |
DT |
denotes |
any |
T13657 |
4492-4494 |
IN |
denotes |
to |
T13656 |
4487-4491 |
NN |
denotes |
rise |
T13655 |
4674-4683 |
VBN |
denotes |
suggested |
T13654 |
4478-4481 |
RB |
denotes |
not |
T13653 |
4474-4477 |
VBD |
denotes |
did |
T13652 |
4459-4464 |
JJ |
denotes |
small |
T13651 |
4450-4458 |
VBN |
denotes |
modified |
T13650 |
4465-4473 |
NNS |
denotes |
subunits |
T13649 |
4444-4449 |
DT |
denotes |
these |
T13648 |
4439-4443 |
IN |
denotes |
with |
T13647 |
4430-4438 |
NNS |
denotes |
calpains |
T13646 |
4427-4429 |
IN |
denotes |
of |
T13645 |
4416-4426 |
NN |
denotes |
expression |
T13644 |
4414-4416 |
, |
denotes |
, |
T13643 |
4482-4486 |
VB |
denotes |
give |
T13642 |
4407-4414 |
RB |
denotes |
However |
T13641 |
4406-4987 |
sentence |
denotes |
However, expression of calpains with these modified small subunits did not give rise to any detectable calpain activity when expressed in E. coli, although their functionality in mammalian cells has yet to be determined (J.S. Elce, unpublished work) It has also been suggested that calpain large subunits alone might provide some activity in eukaryotic cells, although the Zimmerman et al. knockout appears to exclude that possibility, and no calpain activity was observed in our hands when calpain large subunits were expressed alone either in E. coli or in mammalian cells [36]. |
T13640 |
4405-4406 |
. |
denotes |
. |
T13639 |
4397-4405 |
NN |
denotes |
activity |
T13638 |
4389-4396 |
NN |
denotes |
calpain |
T13637 |
4386-4388 |
IN |
denotes |
of |
T13636 |
4376-4379 |
JJ |
denotes |
low |
T13635 |
4380-4385 |
NN |
denotes |
level |
T13634 |
4374-4375 |
DT |
denotes |
a |
T13633 |
4366-4373 |
VB |
denotes |
support |
T13632 |
4363-4365 |
TO |
denotes |
to |
T13631 |
4352-4362 |
JJ |
denotes |
sufficient |
T13630 |
4343-4348 |
MD |
denotes |
might |
T13629 |
4349-4351 |
VB |
denotes |
be |
T13628 |
4337-4342 |
WDT |
denotes |
which |
T13627 |
4335-4337 |
, |
denotes |
, |
T13626 |
4320-4321 |
HYPH |
denotes |
- |
T13625 |
4321-4329 |
JJ |
denotes |
terminal |
T13624 |
4319-4320 |
NN |
denotes |
C |
T13623 |
4305-4318 |
JJ |
denotes |
inappropriate |
T13622 |
4330-4335 |
NNS |
denotes |
acids |
T13621 |
4301-4302 |
SYM |
denotes |
– |
T13620 |
4302-4304 |
CD |
denotes |
30 |
T13619 |
4299-4301 |
CD |
denotes |
10 |
T13618 |
4294-4298 |
IN |
denotes |
with |
T13617 |
4279-4284 |
JJ |
denotes |
small |
T13616 |
4271-4278 |
NN |
denotes |
calpain |
T13615 |
4285-4293 |
NNS |
denotes |
subunits |
T13614 |
4261-4270 |
JJ |
denotes |
defective |
T13613 |
4258-4260 |
IN |
denotes |
to |
T13612 |
4253-4257 |
NN |
denotes |
rise |
T13611 |
4242-4247 |
MD |
denotes |
could |
T13610 |
4248-4252 |
VB |
denotes |
give |
T13609 |
4230-4241 |
NNS |
denotes |
transcripts |
T13608 |
4224-4229 |
DT |
denotes |
These |
T13607 |
4223-4406 |
sentence |
denotes |
These transcripts could give rise to defective calpain small subunits with 10–30 inappropriate C-terminal acids, which might be sufficient to support a low level of calpain activity. |
T13606 |
4222-4223 |
. |
denotes |
. |
T13605 |
4221-4222 |
-RRB- |
denotes |
] |
T13604 |
4219-4221 |
CD |
denotes |
31 |
T13603 |
4218-4219 |
-LRB- |
denotes |
[ |
T13602 |
4200-4208 |
NN |
denotes |
promoter |
T13601 |
4196-4199 |
NN |
denotes |
PGK |
T13600 |
4209-4217 |
NN |
denotes |
sequence |
T13599 |
4192-4195 |
DT |
denotes |
the |
T13598 |
4189-4191 |
IN |
denotes |
in |
T13597 |
4176-4182 |
NN |
denotes |
splice |
T13596 |
4168-4175 |
JJ |
denotes |
cryptic |
T13595 |
4158-4167 |
JJ |
denotes |
different |
T13594 |
4183-4188 |
NNS |
denotes |
sites |
T13593 |
4148-4153 |
RBS |
denotes |
least |
T13592 |
4154-4157 |
CD |
denotes |
two |
T13591 |
4145-4147 |
RB |
denotes |
at |
T13590 |
4142-4144 |
IN |
denotes |
to |
T13589 |
4140-4141 |
CD |
denotes |
9 |
T13588 |
4135-4139 |
NN |
denotes |
exon |
T13587 |
4132-4134 |
IN |
denotes |
of |
T13586 |
4121-4126 |
JJ |
denotes |
first |
T13585 |
4127-4131 |
NN |
denotes |
half |
T13584 |
4117-4120 |
DT |
denotes |
the |
T13583 |
4112-4116 |
IN |
denotes |
from |
T13582 |
4104-4111 |
RB |
denotes |
through |
T13581 |
4096-4103 |
VBG |
denotes |
reading |
T13580 |
4094-4096 |
, |
denotes |
, |
T13579 |
4090-4091 |
HYPH |
denotes |
- |
T13578 |
4091-4094 |
NN |
denotes |
PCR |
T13577 |
4088-4090 |
NN |
denotes |
RT |
T13576 |
4085-4087 |
IN |
denotes |
by |
T13575 |
4074-4084 |
JJ |
denotes |
detectable |
T13574 |
4072-4074 |
, |
denotes |
, |
T13573 |
4060-4064 |
NN |
denotes |
mRNA |
T13572 |
4065-4072 |
NNS |
denotes |
species |
T13571 |
4051-4059 |
JJ |
denotes |
multiple |
T13570 |
4048-4050 |
IN |
denotes |
to |
T13569 |
4043-4047 |
NN |
denotes |
rise |
T13568 |
4038-4042 |
VBD |
denotes |
gave |
T13567 |
4031-4037 |
NN |
denotes |
allele |
T13566 |
4026-4030 |
DT |
denotes |
This |
T13565 |
4025-4223 |
sentence |
denotes |
This allele gave rise to multiple mRNA species, detectable by RT-PCR, reading through from the first half of exon 9 to at least two different cryptic splice sites in the PGK promoter sequence [31]. |
T14160 |
7168-7174 |
IN |
denotes |
beyond |
T14159 |
7156-7167 |
NN |
denotes |
development |
T14158 |
7146-7155 |
JJ |
denotes |
embryonic |
T14157 |
7142-7145 |
IN |
denotes |
for |
T14156 |
7132-7141 |
JJ |
denotes |
essential |
T14155 |
7120-7128 |
NN |
denotes |
activity |
T14154 |
7111-7112 |
HYPH |
denotes |
- |
T14153 |
7112-7119 |
NN |
denotes |
calpain |
T14152 |
7110-7111 |
NN |
denotes |
m |
T14151 |
7129-7131 |
VBZ |
denotes |
is |
T14150 |
7105-7109 |
IN |
denotes |
that |
T14149 |
7082-7083 |
SYM |
denotes |
- |
T14148 |
7081-7082 |
HYPH |
denotes |
/ |
T14147 |
7080-7081 |
SYM |
denotes |
- |
T14146 |
7084-7091 |
NNS |
denotes |
embryos |
T14145 |
7075-7080 |
NN |
denotes |
Capn2 |
T14144 |
7072-7074 |
IN |
denotes |
in |
T14143 |
7062-7071 |
NN |
denotes |
lethality |
T14142 |
7060-7062 |
, |
denotes |
, |
T14141 |
7052-7060 |
NN |
denotes |
contrast |
T14140 |
7092-7104 |
VBZ |
denotes |
demonstrates |
T14139 |
7049-7051 |
IN |
denotes |
In |
T14138 |
7048-7192 |
sentence |
denotes |
In contrast, lethality in Capn2-/- embryos demonstrates that m-calpain activity is essential for embryonic development beyond the 8-cell stage. |
T14137 |
7047-7048 |
. |
denotes |
. |
T14136 |
7038-7047 |
JJ |
denotes |
essential |
T14135 |
7034-7037 |
RB |
denotes |
not |
T14134 |
7027-7033 |
RB |
denotes |
simply |
T14133 |
7014-7015 |
HYPH |
denotes |
- |
T14132 |
7015-7022 |
NN |
denotes |
calpain |
T14131 |
7013-7014 |
NN |
denotes |
μ |
T14130 |
7010-7012 |
IN |
denotes |
of |
T14129 |
7000-7009 |
NNS |
denotes |
functions |
T14128 |
6996-6999 |
DT |
denotes |
the |
T14127 |
7023-7026 |
VBP |
denotes |
are |
T14126 |
6988-6995 |
IN |
denotes |
whether |
T14125 |
6985-6987 |
CC |
denotes |
or |
T14124 |
6983-6985 |
, |
denotes |
, |
T14123 |
6966-6967 |
HYPH |
denotes |
- |
T14122 |
6967-6974 |
NN |
denotes |
calpain |
T14121 |
6965-6966 |
NN |
denotes |
m |
T14120 |
6955-6964 |
VBG |
denotes |
remaining |
T14119 |
6975-6983 |
NN |
denotes |
activity |
T14118 |
6951-6954 |
DT |
denotes |
the |
T14117 |
6948-6950 |
IN |
denotes |
by |
T14116 |
6928-6940 |
JJ |
denotes |
compensatory |
T14115 |
6941-6947 |
NN |
denotes |
affect |
T14114 |
6926-6927 |
DT |
denotes |
a |
T14113 |
6923-6925 |
IN |
denotes |
to |
T14112 |
6919-6922 |
IN |
denotes |
due |
T14111 |
6906-6907 |
SYM |
denotes |
- |
T14110 |
6905-6906 |
HYPH |
denotes |
/ |
T14109 |
6904-6905 |
SYM |
denotes |
- |
T14108 |
6908-6915 |
NNS |
denotes |
animals |
T14107 |
6899-6904 |
NN |
denotes |
Capn1 |
T14106 |
6896-6898 |
IN |
denotes |
in |
T14105 |
6886-6895 |
NN |
denotes |
phenotype |
T14104 |
6879-6885 |
JJ |
denotes |
marked |
T14103 |
6876-6878 |
IN |
denotes |
of |
T14102 |
6868-6875 |
NN |
denotes |
absence |
T14101 |
6864-6867 |
DT |
denotes |
the |
T14100 |
6916-6918 |
VBZ |
denotes |
is |
T14099 |
6856-6863 |
IN |
denotes |
whether |
T14098 |
6846-6855 |
VB |
denotes |
determine |
T14097 |
6843-6845 |
TO |
denotes |
to |
T14096 |
6834-6842 |
JJ |
denotes |
possible |
T14095 |
6830-6833 |
RB |
denotes |
not |
T14094 |
6824-6826 |
PRP |
denotes |
it |
T14093 |
6822-6824 |
, |
denotes |
, |
T14092 |
6816-6822 |
NN |
denotes |
result |
T14091 |
6814-6815 |
DT |
denotes |
a |
T14090 |
6827-6829 |
VBZ |
denotes |
is |
T14089 |
6811-6813 |
IN |
denotes |
As |
T14088 |
6810-7048 |
sentence |
denotes |
As a result, it is not possible to determine whether the absence of marked phenotype in Capn1-/- animals is due to a compensatory affect by the remaining m-calpain activity, or whether the functions of μ-calpain are simply not essential. |
T14087 |
6809-6810 |
. |
denotes |
. |
T14086 |
6799-6803 |
NN |
denotes |
cell |
T14085 |
6804-6809 |
NNS |
denotes |
types |
T14084 |
6793-6798 |
JJ |
denotes |
other |
T14083 |
6790-6792 |
IN |
denotes |
in |
T14082 |
6785-6789 |
IN |
denotes |
than |
T14081 |
6772-6784 |
NNS |
denotes |
erythrocytes |
T14080 |
6768-6771 |
CC |
denotes |
and |
T14079 |
6758-6767 |
NNS |
denotes |
platelets |
T14078 |
6755-6757 |
IN |
denotes |
in |
T14077 |
6748-6754 |
JJ |
denotes |
likely |
T14076 |
6743-6747 |
RBR |
denotes |
less |
T14075 |
6733-6742 |
RB |
denotes |
therefore |
T14074 |
6721-6722 |
HYPH |
denotes |
- |
T14073 |
6722-6729 |
NN |
denotes |
calpain |
T14072 |
6720-6721 |
NN |
denotes |
m |
T14071 |
6717-6719 |
IN |
denotes |
by |
T14070 |
6706-6716 |
NN |
denotes |
deficiency |
T14069 |
6697-6698 |
HYPH |
denotes |
- |
T14068 |
6698-6705 |
NN |
denotes |
calpain |
T14067 |
6696-6697 |
NN |
denotes |
μ |
T14066 |
6692-6695 |
IN |
denotes |
for |
T14065 |
6730-6732 |
VBZ |
denotes |
is |
T14064 |
6679-6691 |
NN |
denotes |
Compensation |
T14063 |
6678-6810 |
sentence |
denotes |
Compensation for μ-calpain deficiency by m-calpain is therefore less likely in platelets and erythrocytes than in other cell types. |
T14062 |
6677-6678 |
. |
denotes |
. |
T14061 |
6667-6677 |
JJ |
denotes |
detectable |
T14060 |
6660-6666 |
RB |
denotes |
barely |
T14059 |
6648-6656 |
NN |
denotes |
activity |
T14058 |
6639-6640 |
HYPH |
denotes |
- |
T14057 |
6640-6647 |
NN |
denotes |
calpain |
T14056 |
6638-6639 |
NN |
denotes |
m |
T14055 |
6657-6659 |
VBZ |
denotes |
is |
T14054 |
6632-6637 |
IN |
denotes |
while |
T14053 |
6630-6632 |
, |
denotes |
, |
T14052 |
6613-6614 |
HYPH |
denotes |
- |
T14051 |
6614-6621 |
NN |
denotes |
calpain |
T14050 |
6612-6613 |
NN |
denotes |
μ |
T14049 |
6622-6630 |
NN |
denotes |
activity |
T14048 |
6603-6611 |
JJ |
denotes |
abundant |
T14047 |
6582-6594 |
NNS |
denotes |
erythrocytes |
T14046 |
6578-6581 |
CC |
denotes |
and |
T14045 |
6595-6602 |
VBP |
denotes |
contain |
T14044 |
6568-6577 |
NNS |
denotes |
Platelets |
T14043 |
6567-6678 |
sentence |
denotes |
Platelets and erythrocytes contain abundant μ-calpain activity, while m-calpain activity is barely detectable. |
T14042 |
6566-6567 |
. |
denotes |
. |
T14041 |
6550-6558 |
JJ |
denotes |
relative |
T13564 |
4024-4025 |
. |
denotes |
. |
T13563 |
4012-4017 |
NN |
denotes |
Capn4 |
T13562 |
4005-4011 |
NNP |
denotes |
Arthur |
T13561 |
4018-4024 |
NN |
denotes |
allele |
T13560 |
4001-4004 |
DT |
denotes |
the |
T13559 |
3996-4000 |
IN |
denotes |
from |
T13558 |
3988-3995 |
VBN |
denotes |
derived |
T13557 |
3977-3987 |
NNS |
denotes |
activities |
T13556 |
3969-3976 |
NN |
denotes |
calpain |
T13555 |
3966-3968 |
CC |
denotes |
or |
T13554 |
3954-3965 |
NNS |
denotes |
transcripts |
T13553 |
3947-3953 |
VB |
denotes |
detect |
T13552 |
3944-3946 |
TO |
denotes |
to |
T13551 |
3934-3938 |
VBD |
denotes |
were |
T13550 |
3939-3943 |
VBN |
denotes |
made |
T13549 |
3926-3933 |
NNS |
denotes |
Efforts |
T13548 |
3925-4025 |
sentence |
denotes |
Efforts were made to detect transcripts or calpain activities derived from the Arthur Capn4 allele. |
T13547 |
3924-3925 |
. |
denotes |
. |
T13546 |
3908-3918 |
JJ |
denotes |
transgenic |
T13545 |
3902-3907 |
NN |
denotes |
Capn4 |
T13544 |
3898-3901 |
CD |
denotes |
two |
T13543 |
3919-3924 |
NNS |
denotes |
lines |
T13542 |
3894-3897 |
DT |
denotes |
the |
T13541 |
3891-3893 |
IN |
denotes |
in |
T13540 |
3886-3890 |
NN |
denotes |
work |
T13539 |
3883-3885 |
IN |
denotes |
at |
T13538 |
3874-3879 |
MD |
denotes |
might |
T13537 |
3880-3882 |
VB |
denotes |
be |
T13536 |
3859-3866 |
JJ |
denotes |
similar |
T13535 |
3867-3873 |
NN |
denotes |
effect |
T13534 |
3857-3858 |
DT |
denotes |
A |
T13533 |
3856-3925 |
sentence |
denotes |
A similar effect might be at work in the two Capn4 transgenic lines. |
T13532 |
3855-3856 |
. |
denotes |
. |
T13531 |
3841-3847 |
NN |
denotes |
mutant |
T13530 |
3848-3855 |
NNS |
denotes |
alleles |
T13523 |
3797-3799 |
IN |
denotes |
of |
T13522 |
3788-3796 |
NN |
denotes |
function |
T13521 |
3785-3787 |
IN |
denotes |
of |
T13520 |
3778-3784 |
NN |
denotes |
degree |
T13519 |
3775-3777 |
IN |
denotes |
in |
T13518 |
3763-3774 |
NNS |
denotes |
differences |
T13517 |
3759-3762 |
DT |
denotes |
the |
T13516 |
3756-3758 |
IN |
denotes |
to |
T13515 |
3741-3744 |
VBD |
denotes |
was |
T13514 |
3731-3740 |
NN |
denotes |
phenotype |
T13513 |
3728-3730 |
IN |
denotes |
in |
T13512 |
3745-3755 |
VBN |
denotes |
attributed |
T13511 |
3718-3727 |
NN |
denotes |
variation |
T13510 |
3714-3717 |
DT |
denotes |
The |
T13509 |
3713-3856 |
sentence |
denotes |
The variation in phenotype was attributed to the differences in degree of function of the truncated proteins produced from the mutant alleles. |
T13508 |
3712-3713 |
. |
denotes |
. |
T13507 |
3711-3712 |
-RRB- |
denotes |
] |
T13506 |
3709-3711 |
CD |
denotes |
35 |
T13505 |
3708-3709 |
-LRB- |
denotes |
[ |
T13504 |
3706-3707 |
-RRB- |
denotes |
) |
T13503 |
3699-3701 |
, |
denotes |
, |
T13502 |
3694-3699 |
NN |
denotes |
E10.5 |
T13501 |
3692-3694 |
, |
denotes |
, |
T13500 |
3688-3692 |
NN |
denotes |
E0.5 |
T13499 |
3701-3706 |
NN |
denotes |
E14.5 |
T13498 |
3687-3688 |
-LRB- |
denotes |
( |
T13497 |
3680-3686 |
NNS |
denotes |
stages |
T13496 |
3670-3679 |
JJ |
denotes |
different |
T13495 |
3667-3669 |
IN |
denotes |
at |
T13494 |
3663-3666 |
CC |
denotes |
but |
T13493 |
3661-3663 |
, |
denotes |
, |
T13492 |
3648-3661 |
NN |
denotes |
embryogenesis |
T13491 |
3641-3647 |
IN |
denotes |
during |
T13490 |
3624-3631 |
NNS |
denotes |
embryos |
T13489 |
3619-3623 |
JJ |
denotes |
null |
T13488 |
3608-3618 |
JJ |
denotes |
homozygous |
T13487 |
3606-3608 |
, |
denotes |
, |
T13486 |
3593-3598 |
CD |
denotes |
three |
T13485 |
3599-3606 |
NNS |
denotes |
studies |
T13484 |
3589-3592 |
DT |
denotes |
all |
T13483 |
3632-3640 |
VBN |
denotes |
perished |
T13482 |
3586-3588 |
IN |
denotes |
In |
T13481 |
3585-3713 |
sentence |
denotes |
In all three studies, homozygous null embryos perished during embryogenesis, but at different stages (E0.5, E10.5, E14.5) [35]. |
T13480 |
3584-3585 |
. |
denotes |
. |
T13479 |
3578-3579 |
-RRB- |
denotes |
) |
T13478 |
3575-3578 |
NN |
denotes |
Mll |
T13477 |
3574-3575 |
-LRB- |
denotes |
( |
T13476 |
3557-3564 |
NN |
denotes |
lineage |
T13475 |
3565-3573 |
NN |
denotes |
leukemia |
T13474 |
3551-3556 |
JJ |
denotes |
mixed |
T13473 |
3580-3584 |
NN |
denotes |
gene |
T13472 |
3547-3550 |
DT |
denotes |
the |
T13471 |
3539-3546 |
VB |
denotes |
disrupt |
T13470 |
3536-3538 |
TO |
denotes |
to |
T13469 |
3517-3530 |
RB |
denotes |
independently |
T13468 |
3512-3516 |
VBD |
denotes |
were |
T13467 |
3491-3500 |
NN |
denotes |
targeting |
T13466 |
3481-3490 |
JJ |
denotes |
different |
T13465 |
3501-3511 |
NNS |
denotes |
strategies |
T13464 |
3475-3480 |
CD |
denotes |
three |
T13463 |
3473-3475 |
, |
denotes |
, |
T13462 |
3466-3473 |
NN |
denotes |
example |
T13461 |
3531-3535 |
VBN |
denotes |
used |
T13460 |
3462-3465 |
IN |
denotes |
For |
T13459 |
3461-3585 |
sentence |
denotes |
For example, three different targeting strategies were independently used to disrupt the mixed lineage leukemia (Mll) gene. |
T13458 |
3460-3461 |
. |
denotes |
. |
T13457 |
3453-3460 |
NNS |
denotes |
studies |
T13456 |
3442-3452 |
NN |
denotes |
disruption |
T13455 |
3437-3441 |
NN |
denotes |
gene |
T13454 |
3434-3436 |
IN |
denotes |
in |
T13453 |
3423-3433 |
NNS |
denotes |
phenotypes |
T13452 |
3413-3422 |
JJ |
denotes |
different |
T13451 |
3407-3412 |
VB |
denotes |
yield |
T13450 |
3404-3406 |
TO |
denotes |
to |
T13449 |
3393-3397 |
VBN |
denotes |
been |
T13448 |
3388-3392 |
VBP |
denotes |
have |
T13447 |
3398-3403 |
VBN |
denotes |
shown |
T13446 |
3367-3376 |
NN |
denotes |
targeting |
T13445 |
3377-3387 |
NNS |
denotes |
strategies |
T13444 |
3357-3366 |
JJ |
denotes |
Alternate |
T13443 |
3356-3461 |
sentence |
denotes |
Alternate targeting strategies have been shown to yield different phenotypes in gene disruption studies. |
T13442 |
3355-3356 |
. |
denotes |
. |
T13441 |
3335-3346 |
JJ |
denotes |
hypomorphic |
T13440 |
3347-3355 |
NN |
denotes |
mutation |
T13439 |
3333-3334 |
DT |
denotes |
a |
T13438 |
3326-3332 |
RB |
denotes |
likely |
T13437 |
3309-3315 |
NNP |
denotes |
Arthur |
T13436 |
3316-3322 |
NN |
denotes |
allele |
T13435 |
3305-3308 |
DT |
denotes |
the |
T13434 |
3323-3325 |
VBZ |
denotes |
is |
T13433 |
3299-3304 |
IN |
denotes |
while |
T13432 |
3285-3289 |
JJ |
denotes |
null |
T13431 |
3280-3284 |
JJ |
denotes |
true |
T13430 |
3290-3298 |
NN |
denotes |
genotype |
T13429 |
3278-3279 |
DT |
denotes |
a |
T13428 |
3258-3266 |
RB |
denotes |
probably |
T13427 |
3267-3277 |
VBZ |
denotes |
represents |
T13426 |
3241-3250 |
NNP |
denotes |
Zimmerman |
T13425 |
3251-3257 |
NN |
denotes |
allele |
T13424 |
3237-3240 |
DT |
denotes |
The |
T13423 |
3236-3356 |
sentence |
denotes |
The Zimmerman allele probably represents a true null genotype while the Arthur allele is likely a hypomorphic mutation. |
T13422 |
3235-3236 |
. |
denotes |
. |
T13421 |
3225-3235 |
NN |
denotes |
disruption |
T13420 |
3222-3224 |
IN |
denotes |
of |
T13419 |
3214-3221 |
NNS |
denotes |
extents |
T13418 |
3204-3213 |
JJ |
denotes |
different |
T13417 |
3201-3203 |
IN |
denotes |
by |
T13416 |
3188-3190 |
VB |
denotes |
be |
T13415 |
3184-3187 |
MD |
denotes |
can |
T13414 |
3172-3177 |
NN |
denotes |
Capn4 |
T13413 |
3163-3171 |
VBN |
denotes |
targeted |
T13412 |
3159-3162 |
CD |
denotes |
two |
T13411 |
3178-3183 |
NNS |
denotes |
lines |
T13410 |
3153-3158 |
DT |
denotes |
these |
T13409 |
3150-3152 |
IN |
denotes |
of |
T13408 |
3140-3149 |
NN |
denotes |
lethality |
T13407 |
3137-3139 |
IN |
denotes |
of |
T13406 |
3132-3136 |
NN |
denotes |
time |
T13405 |
3128-3131 |
DT |
denotes |
the |
T13404 |
3125-3127 |
IN |
denotes |
in |
T13403 |
3114-3124 |
NN |
denotes |
difference |
T13402 |
3110-3113 |
DT |
denotes |
the |
T13401 |
3191-3200 |
VBN |
denotes |
explained |
T13400 |
3105-3109 |
IN |
denotes |
that |
T13399 |
3096-3104 |
JJ |
denotes |
probable |
T13398 |
3086-3089 |
RB |
denotes |
now |
T13397 |
3090-3095 |
VBZ |
denotes |
seems |
T13396 |
3083-3085 |
PRP |
denotes |
It |
T13395 |
3082-3236 |
sentence |
denotes |
It now seems probable that the difference in the time of lethality of these two targeted Capn4 lines can be explained by different extents of disruption. |
T13394 |
3081-3082 |
. |
denotes |
. |
T13393 |
3080-3081 |
-RRB- |
denotes |
] |
T13392 |
3078-3080 |
CD |
denotes |
32 |
T13391 |
3077-3078 |
-LRB- |
denotes |
[ |
T13390 |
3075-3076 |
CD |
denotes |
8 |
T13389 |
3067-3074 |
IN |
denotes |
through |
T13388 |
3065-3066 |
CD |
denotes |
4 |
T13387 |
3059-3064 |
NNS |
denotes |
exons |
T13386 |
3056-3058 |
IN |
denotes |
of |
T13385 |
3037-3046 |
JJ |
denotes |
extensive |
T13384 |
3032-3036 |
RBR |
denotes |
more |
T13383 |
3027-3031 |
RB |
denotes |
much |
T13382 |
3047-3055 |
NN |
denotes |
deletion |
T13381 |
3025-3026 |
DT |
denotes |
a |
T13380 |
3005-3015 |
NNS |
denotes |
colleagues |
T13379 |
3001-3004 |
CC |
denotes |
and |
T13378 |
2991-3000 |
NNP |
denotes |
Zimmerman |
T13377 |
2988-2990 |
IN |
denotes |
by |
T13376 |
2979-2987 |
VBN |
denotes |
employed |
T13375 |
2960-2969 |
VBG |
denotes |
targeting |
T13374 |
2954-2959 |
NN |
denotes |
Capn4 |
T13373 |
2970-2978 |
NN |
denotes |
strategy |
T13372 |
2950-2953 |
DT |
denotes |
the |
T13371 |
2948-2950 |
, |
denotes |
, |
T13370 |
2940-2948 |
NN |
denotes |
contrast |
T13369 |
3016-3024 |
VBD |
denotes |
involved |
T13368 |
2937-2939 |
IN |
denotes |
In |
T13367 |
2936-3082 |
sentence |
denotes |
In contrast, the Capn4 targeting strategy employed by Zimmerman and colleagues involved a much more extensive deletion of exons 4 through 8 [32]. |
T13366 |
2935-2936 |
. |
denotes |
. |
T13365 |
2934-2935 |
-RRB- |
denotes |
] |
T13364 |
2932-2934 |
CD |
denotes |
34 |
T13363 |
2931-2932 |
-LRB- |
denotes |
[ |
T13362 |
2926-2930 |
NNP |
denotes |
coli |
T13361 |
2923-2925 |
NNP |
denotes |
E. |
T13360 |
2920-2922 |
IN |
denotes |
in |
T13359 |
2906-2911 |
JJ |
denotes |
large |
T13358 |
2897-2898 |
HYPH |
denotes |
- |
T13357 |
2898-2905 |
NN |
denotes |
calpain |
T13356 |
2896-2897 |
NN |
denotes |
m |
T13355 |
2912-2919 |
NN |
denotes |
subunit |
T13354 |
2892-2895 |
DT |
denotes |
the |
T13353 |
2887-2891 |
IN |
denotes |
with |
T13352 |
2874-2886 |
VBN |
denotes |
co-expressed |
T13351 |
2869-2873 |
WRB |
denotes |
when |
T13350 |
2860-2868 |
NN |
denotes |
activity |
T13349 |
2852-2859 |
NN |
denotes |
calpain |
T13348 |
2828-2833 |
JJ |
denotes |
small |
T13347 |
2834-2841 |
NN |
denotes |
subunit |
T13346 |
2824-2827 |
DT |
denotes |
the |
T13345 |
2821-2823 |
IN |
denotes |
of |
T13344 |
2812-2820 |
VBZ |
denotes |
residues |
T13343 |
2801-2806 |
NN |
denotes |
amino |
T13824 |
5408-5410 |
CD |
denotes |
62 |
T13823 |
5402-5407 |
VBP |
denotes |
share |
T13822 |
5390-5393 |
CD |
denotes |
two |
T13821 |
5394-5401 |
NNS |
denotes |
enzymes |
T13820 |
5386-5389 |
DT |
denotes |
The |
T13819 |
5385-5487 |
sentence |
denotes |
The two enzymes share 62% sequence identity and are very similar in their structure and biochemistry. |
T13818 |
5384-5385 |
. |
denotes |
. |
T13817 |
5374-5378 |
FW |
denotes |
vivo |
T13816 |
5371-5373 |
FW |
denotes |
in |
T13815 |
5379-5384 |
NNS |
denotes |
roles |
T13814 |
5362-5370 |
JJ |
denotes |
distinct |
T13813 |
5352-5354 |
, |
denotes |
, |
T13812 |
5344-5345 |
HYPH |
denotes |
- |
T13811 |
5343-5344 |
NN |
denotes |
m |
T13810 |
5339-5342 |
CC |
denotes |
and |
T13809 |
5337-5338 |
HYPH |
denotes |
- |
T13808 |
5345-5352 |
NN |
denotes |
calpain |
T13807 |
5336-5337 |
NN |
denotes |
μ |
T13806 |
5334-5336 |
, |
denotes |
, |
T13805 |
5315-5325 |
JJ |
denotes |
ubiquitous |
T13804 |
5311-5314 |
CD |
denotes |
two |
T13803 |
5326-5334 |
NNS |
denotes |
isoforms |
T13802 |
5307-5310 |
DT |
denotes |
the |
T13801 |
5354-5361 |
VBP |
denotes |
possess |
T13800 |
5299-5306 |
IN |
denotes |
whether |
T13799 |
5290-5293 |
VBZ |
denotes |
has |
T13798 |
5281-5289 |
NN |
denotes |
research |
T13797 |
5273-5280 |
NN |
denotes |
calpain |
T13796 |
5270-5272 |
IN |
denotes |
in |
T13795 |
5251-5259 |
VBG |
denotes |
enduring |
T13794 |
5260-5269 |
NNS |
denotes |
questions |
T13793 |
5247-5250 |
DT |
denotes |
the |
T13792 |
5244-5246 |
IN |
denotes |
of |
T13791 |
5294-5298 |
VBN |
denotes |
been |
T13790 |
5240-5243 |
CD |
denotes |
One |
T13789 |
5239-5385 |
sentence |
denotes |
One of the enduring questions in calpain research has been whether the two ubiquitous isoforms, μ- and m-calpain, possess distinct in vivo roles. |
T13788 |
5238-5239 |
. |
denotes |
. |
T13787 |
5237-5238 |
-RRB- |
denotes |
] |
T13786 |
5235-5237 |
CD |
denotes |
37 |
T13785 |
5234-5235 |
-LRB- |
denotes |
[ |
T13784 |
5224-5233 |
NNS |
denotes |
occasions |
T13783 |
5221-5223 |
IN |
denotes |
of |
T13782 |
5214-5220 |
NN |
denotes |
number |
T13781 |
5212-5213 |
DT |
denotes |
a |
T13780 |
5209-5211 |
IN |
denotes |
on |
T13779 |
5204-5208 |
NNS |
denotes |
mice |
T13778 |
5193-5203 |
JJ |
denotes |
transgenic |
T13777 |
5190-5192 |
IN |
denotes |
of |
T13776 |
5180-5189 |
NN |
denotes |
phenotype |
T13775 |
5176-5179 |
DT |
denotes |
the |
T13774 |
5166-5175 |
VB |
denotes |
influence |
T13773 |
5163-5165 |
TO |
denotes |
to |
T13772 |
5149-5153 |
VBN |
denotes |
been |
T13771 |
5145-5148 |
VBZ |
denotes |
has |
T13770 |
5154-5162 |
VBN |
denotes |
observed |
T13769 |
5139-5144 |
WDT |
denotes |
which |
T13768 |
5137-5139 |
, |
denotes |
, |
T13767 |
5121-5131 |
JJ |
denotes |
transgenic |
T13766 |
5117-5120 |
CD |
denotes |
two |
T13765 |
5132-5137 |
NNS |
denotes |
lines |
T13764 |
5113-5116 |
DT |
denotes |
the |
T13763 |
5110-5112 |
IN |
denotes |
of |
T13762 |
5090-5097 |
JJ |
denotes |
genetic |
T13761 |
5080-5089 |
JJ |
denotes |
different |
T13760 |
5098-5109 |
NNS |
denotes |
backgrounds |
T13759 |
5076-5079 |
DT |
denotes |
the |
T13758 |
5073-5075 |
IN |
denotes |
of |
T13757 |
5061-5072 |
NN |
denotes |
consequence |
T13756 |
5059-5060 |
DT |
denotes |
a |
T13755 |
5051-5055 |
RB |
denotes |
also |
T13754 |
5045-5050 |
MD |
denotes |
might |
T13753 |
5029-5034 |
NN |
denotes |
Capn4 |
T13752 |
5035-5044 |
NNS |
denotes |
knockouts |
T13751 |
5025-5028 |
DT |
denotes |
the |
T13750 |
5022-5024 |
IN |
denotes |
in |
T13749 |
5012-5021 |
NN |
denotes |
lethality |
T13748 |
5009-5011 |
IN |
denotes |
of |
T13747 |
5056-5058 |
VB |
denotes |
be |
T13746 |
4992-5001 |
JJ |
denotes |
different |
T13342 |
2798-2800 |
CD |
denotes |
25 |
T13341 |
2788-2789 |
HYPH |
denotes |
- |
T13340 |
2789-2797 |
JJ |
denotes |
terminal |
T13339 |
2787-2788 |
NN |
denotes |
C |
T13338 |
2807-2811 |
NN |
denotes |
acid |
T13337 |
2783-2786 |
DT |
denotes |
the |
T13336 |
2780-2782 |
IN |
denotes |
of |
T13335 |
2771-2779 |
NN |
denotes |
excision |
T13334 |
2842-2851 |
VBD |
denotes |
abolished |
T13333 |
2766-2770 |
IN |
denotes |
that |
T13332 |
2758-2765 |
VBG |
denotes |
showing |
T13331 |
2740-2741 |
HYPH |
denotes |
/ |
T13330 |
2741-2749 |
NN |
denotes |
function |
T13329 |
2731-2740 |
NN |
denotes |
structure |
T13328 |
2750-2757 |
NNS |
denotes |
studies |
T13327 |
2722-2730 |
JJ |
denotes |
previous |
T13326 |
2717-2721 |
IN |
denotes |
upon |
T13325 |
2707-2710 |
VBD |
denotes |
was |
T13324 |
2711-2716 |
VBN |
denotes |
based |
T13323 |
2698-2706 |
NN |
denotes |
strategy |
T13322 |
2693-2697 |
DT |
denotes |
This |
T13321 |
2692-2936 |
sentence |
denotes |
This strategy was based upon previous structure/function studies showing that excision of the C-terminal 25 amino acid residues of the small subunit abolished calpain activity when co-expressed with the m-calpain large subunit in E. coli [34]. |
T13320 |
2691-2692 |
. |
denotes |
. |
T13319 |
2690-2691 |
-RRB- |
denotes |
] |
T13318 |
2688-2690 |
CD |
denotes |
31 |
T13317 |
2687-2688 |
-LRB- |
denotes |
[ |
T13316 |
2679-2686 |
NN |
denotes |
protein |
T13315 |
2675-2678 |
DT |
denotes |
the |
T13314 |
2672-2674 |
IN |
denotes |
of |
T13313 |
2661-2671 |
NN |
denotes |
truncation |
T13312 |
2654-2660 |
VBD |
denotes |
caused |
T13311 |
2648-2653 |
WDT |
denotes |
which |
T13310 |
2646-2648 |
, |
denotes |
, |
T13309 |
2645-2646 |
CD |
denotes |
9 |
T13308 |
2640-2644 |
NN |
denotes |
exon |
T13282 |
2495-2497 |
IN |
denotes |
by |
T13281 |
2486-2494 |
VBN |
denotes |
employed |
T13280 |
2520-2528 |
VBN |
denotes |
involved |
T13279 |
2467-2476 |
NN |
denotes |
targeting |
T13278 |
2461-2466 |
NN |
denotes |
Capn4 |
T13277 |
2477-2485 |
NN |
denotes |
strategy |
T13276 |
2457-2460 |
DT |
denotes |
The |
T13275 |
2456-2692 |
sentence |
denotes |
The Capn4 targeting strategy employed by Arthur and colleagues involved disrupting the C-terminus of the calpain small subunit by insertion of the PGK-Neo cassette into the middle of exon 9, which caused truncation of the protein [31]. |
T13274 |
2455-2456 |
. |
denotes |
. |
T13273 |
2446-2450 |
JJ |
denotes |
true |
T13272 |
2451-2455 |
NN |
denotes |
null |
T13271 |
2444-2445 |
DT |
denotes |
a |
T13270 |
2439-2443 |
IN |
denotes |
than |
T13269 |
2432-2438 |
RB |
denotes |
rather |
T13268 |
2430-2432 |
, |
denotes |
, |
T13267 |
2413-2424 |
JJ |
denotes |
hypomorphic |
T13266 |
2425-2430 |
NN |
denotes |
state |
T13265 |
2411-2412 |
DT |
denotes |
a |
T13264 |
2388-2394 |
JJ |
denotes |
latter |
T13263 |
2395-2399 |
NN |
denotes |
line |
T13262 |
2384-2387 |
DT |
denotes |
the |
T13261 |
2400-2410 |
VBZ |
denotes |
represents |
T13260 |
2379-2383 |
IN |
denotes |
that |
T13259 |
2372-2378 |
JJ |
denotes |
likely |
T13258 |
2363-2365 |
PRP |
denotes |
it |
T13257 |
2361-2363 |
, |
denotes |
, |
T13256 |
2351-2361 |
NN |
denotes |
retrospect |
T13255 |
2366-2371 |
VBZ |
denotes |
seems |
T13254 |
2348-2350 |
IN |
denotes |
In |
T13253 |
2347-2456 |
sentence |
denotes |
In retrospect, it seems likely that the latter line represents a hypomorphic state, rather than a true null. |
T13252 |
2346-2347 |
. |
denotes |
. |
T13251 |
2345-2346 |
-RRB- |
denotes |
] |
T13250 |
2343-2345 |
CD |
denotes |
31 |
T13249 |
2342-2343 |
-LRB- |
denotes |
[ |
T13248 |
2332-2341 |
VB |
denotes |
reconcile |
T13247 |
2329-2331 |
TO |
denotes |
to |
T13246 |
2319-2328 |
JJ |
denotes |
difficult |
T13245 |
2314-2318 |
RBR |
denotes |
more |
T13244 |
2297-2310 |
NN |
denotes |
mid-gestation |
T13243 |
2294-2296 |
IN |
denotes |
to |
T13242 |
2283-2293 |
NNS |
denotes |
colleagues |
T13241 |
2279-2282 |
CC |
denotes |
and |
T13240 |
2272-2278 |
NNP |
denotes |
Arthur |
T13239 |
2269-2271 |
IN |
denotes |
by |
T13238 |
2260-2268 |
VBN |
denotes |
reported |
T13237 |
2246-2254 |
VBN |
denotes |
targeted |
T13236 |
2237-2245 |
JJ |
denotes |
original |
T13235 |
2255-2259 |
NN |
denotes |
line |
T13234 |
2233-2236 |
DT |
denotes |
the |
T13233 |
2228-2232 |
IN |
denotes |
from |
T13232 |
2218-2219 |
SYM |
denotes |
- |
T13231 |
2217-2218 |
HYPH |
denotes |
/ |
T13230 |
2216-2217 |
SYM |
denotes |
- |
T13229 |
2220-2227 |
NNS |
denotes |
embryos |
T13228 |
2211-2216 |
NN |
denotes |
Capn4 |
T13227 |
2208-2210 |
IN |
denotes |
of |
T13226 |
2311-2313 |
VBZ |
denotes |
is |
T13225 |
2199-2207 |
NN |
denotes |
survival |
T13224 |
2195-2198 |
DT |
denotes |
The |
T13223 |
2194-2347 |
sentence |
denotes |
The survival of Capn4-/- embryos from the original targeted line reported by Arthur and colleagues to mid-gestation is more difficult to reconcile [31]. |
T13222 |
2193-2194 |
. |
denotes |
. |
T13221 |
2192-2193 |
-RRB- |
denotes |
] |
T13220 |
2190-2192 |
CD |
denotes |
32 |
T13219 |
2189-2190 |
-LRB- |
denotes |
[ |
T13218 |
2175-2179 |
RB |
denotes |
also |
T13217 |
2171-2174 |
VBD |
denotes |
was |
T13216 |
2163-2170 |
NNS |
denotes |
embryos |
T13215 |
2158-2162 |
JJ |
denotes |
null |
T13214 |
2155-2157 |
IN |
denotes |
of |
T13213 |
2145-2154 |
NN |
denotes |
lethality |
T13212 |
2129-2144 |
NN |
denotes |
preimplantation |
T13211 |
2123-2128 |
WDT |
denotes |
which |
T13210 |
2180-2188 |
VBN |
denotes |
observed |
T13209 |
2120-2122 |
IN |
denotes |
in |
T13208 |
2105-2113 |
VBN |
denotes |
targeted |
T13207 |
2099-2104 |
NN |
denotes |
Capn4 |
T13206 |
2095-2098 |
CD |
denotes |
two |
T13205 |
2114-2119 |
NNS |
denotes |
lines |
T13204 |
2091-2094 |
DT |
denotes |
the |
T13203 |
2088-2090 |
IN |
denotes |
of |
T13202 |
2084-2087 |
CD |
denotes |
one |
T13201 |
2081-2083 |
IN |
denotes |
by |
T13200 |
2071-2080 |
VBN |
denotes |
presented |
T13199 |
2061-2070 |
NN |
denotes |
phenotype |
T13198 |
2057-2060 |
DT |
denotes |
the |
T13197 |
2052-2056 |
IN |
denotes |
with |
T13196 |
2042-2051 |
NN |
denotes |
agreement |
T13195 |
2039-2041 |
IN |
denotes |
in |
T13194 |
2029-2035 |
NN |
denotes |
result |
T13193 |
2024-2028 |
DT |
denotes |
this |
T13192 |
2022-2024 |
, |
denotes |
, |
T13191 |
2014-2022 |
NN |
denotes |
activity |
T13190 |
2005-2006 |
HYPH |
denotes |
- |
T13189 |
2006-2013 |
NN |
denotes |
calpain |
T13188 |
2004-2005 |
NN |
denotes |
m |
T13187 |
1996-2003 |
VB |
denotes |
abolish |
T13186 |
1993-1995 |
TO |
denotes |
to |
T13185 |
2036-2038 |
VBZ |
denotes |
is |
T13184 |
1979-1983 |
RB |
denotes |
also |
T13183 |
1975-1978 |
VBD |
denotes |
was |
T13182 |
1964-1969 |
NN |
denotes |
Capn4 |
T13181 |
1970-1974 |
NN |
denotes |
gene |
T13180 |
1960-1963 |
DT |
denotes |
the |
T13179 |
1957-1959 |
IN |
denotes |
of |
T13178 |
1946-1956 |
NN |
denotes |
disruption |
T13177 |
1935-1945 |
JJ |
denotes |
homozygous |
T13176 |
1984-1992 |
VBN |
denotes |
expected |
T13175 |
1929-1934 |
IN |
denotes |
Since |
T13174 |
1928-2194 |
sentence |
denotes |
Since homozygous disruption of the Capn4 gene was also expected to abolish m-calpain activity, this result is in agreement with the phenotype presented by one of the two Capn4 targeted lines in which preimplantation lethality of null embryos was also observed [32]. |
T13173 |
1927-1928 |
. |
denotes |
. |
T13172 |
1914-1927 |
NN |
denotes |
embryogenesis |
T13171 |
1907-1913 |
IN |
denotes |
during |
T13170 |
1885-1897 |
RB |
denotes |
specifically |
T13169 |
1882-1884 |
VBZ |
denotes |
is |
T13168 |
1873-1874 |
HYPH |
denotes |
- |
T13167 |
1874-1881 |
NN |
denotes |
calpain |
T13166 |
1872-1873 |
NN |
denotes |
m |
T13165 |
1898-1906 |
VBN |
denotes |
required |
T13164 |
1867-1871 |
IN |
denotes |
that |
T13163 |
1854-1857 |
RB |
denotes |
now |
T13162 |
1851-1853 |
TO |
denotes |
to |
T13161 |
1858-1866 |
VB |
denotes |
conclude |
T13160 |
1848-1850 |
PRP |
denotes |
us |
T13159 |
1841-1847 |
VBZ |
denotes |
allows |
T13158 |
1829-1840 |
NN |
denotes |
observation |
T13157 |
1824-1828 |
DT |
denotes |
This |
T13156 |
1823-1928 |
sentence |
denotes |
This observation allows us to now conclude that m-calpain is specifically required during embryogenesis. |
T13155 |
1822-1823 |
. |
denotes |
. |
T13154 |
1811-1822 |
NN |
denotes |
development |
T13153 |
1808-1810 |
IN |
denotes |
of |
T13152 |
1786-1801 |
NN |
denotes |
preimplantation |
T13151 |
1802-1807 |
NN |
denotes |
stage |
T13150 |
1782-1785 |
DT |
denotes |
the |
T13149 |
1779-1781 |
IN |
denotes |
at |
T13148 |
1773-1775 |
, |
denotes |
, |
T13147 |
1756-1765 |
JJ |
denotes |
catalytic |
T13146 |
1747-1748 |
HYPH |
denotes |
- |
T13145 |
1748-1755 |
NN |
denotes |
calpain |
T13144 |
1746-1747 |
NN |
denotes |
m |
T13143 |
1766-1773 |
NN |
denotes |
subunit |
T13142 |
1742-1745 |
DT |
denotes |
the |
T13141 |
1737-1741 |
VBP |
denotes |
lack |
T13140 |
1731-1736 |
WDT |
denotes |
which |
T13139 |
1729-1731 |
, |
denotes |
, |
T13138 |
1723-1724 |
SYM |
denotes |
- |
T13137 |
1722-1723 |
HYPH |
denotes |
/ |
T13136 |
1721-1722 |
SYM |
denotes |
- |
T13135 |
1725-1729 |
NNS |
denotes |
mice |
T13134 |
1716-1721 |
NN |
denotes |
Capn2 |
T13133 |
1775-1778 |
VBP |
denotes |
die |
T13132 |
1711-1715 |
IN |
denotes |
that |
T13131 |
1706-1710 |
RB |
denotes |
here |
T13130 |
1699-1705 |
VBP |
denotes |
report |
T13129 |
1696-1698 |
PRP |
denotes |
We |
T13128 |
1695-1823 |
sentence |
denotes |
We report here that Capn2-/- mice, which lack the m-calpain catalytic subunit, die at the preimplantation stage of development. |
T13127 |
1694-1695 |
. |
denotes |
. |
T13126 |
1685-1694 |
NN |
denotes |
viability |
T13125 |
1675-1684 |
JJ |
denotes |
embryonic |
T13124 |
1664-1674 |
VBG |
denotes |
sustaining |
T13123 |
1660-1663 |
IN |
denotes |
for |
T13122 |
1649-1659 |
JJ |
denotes |
sufficient |
T13121 |
1639-1644 |
RB |
denotes |
alone |
T13120 |
1631-1638 |
NN |
denotes |
isoform |
T13119 |
1624-1630 |
DT |
denotes |
either |
T13118 |
1645-1648 |
VBD |
denotes |
was |
T13117 |
1619-1623 |
IN |
denotes |
that |
T13116 |
1616-1618 |
CC |
denotes |
or |
T13115 |
1614-1616 |
, |
denotes |
, |
T13114 |
1601-1614 |
NN |
denotes |
embryogenesis |
T13113 |
1594-1600 |
IN |
denotes |
during |
T13112 |
1572-1584 |
RB |
denotes |
specifically |
T13111 |
1568-1571 |
VBD |
denotes |
was |
T13110 |
1559-1560 |
HYPH |
denotes |
- |
T13109 |
1558-1559 |
NN |
denotes |
m |
T13108 |
1560-1567 |
NN |
denotes |
calpain |
T13107 |
1551-1557 |
DT |
denotes |
either |
T13106 |
1585-1593 |
VBN |
denotes |
required |
T13105 |
1546-1550 |
IN |
denotes |
that |
T13104 |
1528-1531 |
CD |
denotes |
two |
T13103 |
1523-1527 |
JJ |
denotes |
last |
T13102 |
1532-1545 |
NNS |
denotes |
possibilities |
T13529 |
3837-3840 |
DT |
denotes |
the |
T13528 |
3832-3836 |
IN |
denotes |
from |
T13527 |
3823-3831 |
VBN |
denotes |
produced |
T13526 |
3804-3813 |
VBN |
denotes |
truncated |
T13525 |
3814-3822 |
NN |
denotes |
proteins |
T13524 |
3800-3803 |
DT |
denotes |
the |
T13101 |
1519-1522 |
DT |
denotes |
the |
T13100 |
1514-1518 |
IN |
denotes |
with |
T13099 |
1504-1508 |
VBD |
denotes |
were |
T13098 |
1501-1503 |
PRP |
denotes |
we |
T13097 |
1499-1501 |
, |
denotes |
, |
T13096 |
1494-1499 |
NN |
denotes |
point |
T13095 |
1489-1493 |
DT |
denotes |
that |
T13094 |
1509-1513 |
VBN |
denotes |
left |
T13093 |
1486-1488 |
IN |
denotes |
At |
T13092 |
1485-1695 |
sentence |
denotes |
At that point, we were left with the last two possibilities that either m-calpain was specifically required during embryogenesis, or that either isoform alone was sufficient for sustaining embryonic viability. |
T13091 |
1484-1485 |
. |
denotes |
. |
T13090 |
1483-1484 |
-RRB- |
denotes |
] |
T13089 |
1481-1483 |
CD |
denotes |
33 |
T13088 |
1480-1481 |
-LRB- |
denotes |
[ |
T13087 |
1466-1470 |
VBD |
denotes |
were |
T13086 |
1447-1457 |
NN |
denotes |
retraction |
T13085 |
1442-1446 |
NN |
denotes |
clot |
T13084 |
1438-1441 |
CC |
denotes |
and |
T13083 |
1458-1465 |
NNS |
denotes |
defects |
T13082 |
1426-1437 |
NN |
denotes |
aggregation |
T13081 |
1417-1425 |
NN |
denotes |
platelet |
T13080 |
1471-1479 |
VBN |
denotes |
observed |
T13079 |
1408-1416 |
IN |
denotes |
although |
T13078 |
1406-1408 |
, |
denotes |
, |
T13077 |
1399-1406 |
JJ |
denotes |
fertile |
T13076 |
1395-1398 |
CC |
denotes |
and |
T13075 |
1387-1394 |
JJ |
denotes |
healthy |
T13074 |
1380-1382 |
, |
denotes |
, |
T13073 |
1363-1372 |
JJ |
denotes |
catalytic |
T13072 |
1354-1355 |
HYPH |
denotes |
- |
T13071 |
1355-1362 |
NN |
denotes |
calpain |
T13070 |
1353-1354 |
NN |
denotes |
μ |
T13069 |
1373-1380 |
NN |
denotes |
subunit |
T13068 |
1349-1352 |
DT |
denotes |
the |
T13067 |
1344-1348 |
VBP |
denotes |
lack |
T12957 |
816-824 |
NN |
denotes |
knockout |
T12956 |
825-829 |
NNS |
denotes |
mice |
T12955 |
810-815 |
NN |
denotes |
Capn4 |
T12954 |
807-809 |
IN |
denotes |
in |
T12953 |
797-806 |
NN |
denotes |
lethality |
T12952 |
787-796 |
JJ |
denotes |
embryonic |
T12951 |
778-786 |
VBD |
denotes |
observed |
T12950 |
753-764 |
JJ |
denotes |
independent |
T12949 |
765-777 |
NNS |
denotes |
laboratories |
T12948 |
749-752 |
CD |
denotes |
Two |
T12947 |
748-881 |
sentence |
denotes |
Two independent laboratories observed embryonic lethality in Capn4 knockout mice, albeit at different stages of development [31,32]. |
T12946 |
747-748 |
. |
denotes |
. |
T12945 |
739-740 |
HYPH |
denotes |
- |
T12944 |
738-739 |
NN |
denotes |
m |
T12943 |
734-737 |
CC |
denotes |
and |
T12942 |
732-733 |
HYPH |
denotes |
- |
T12941 |
731-732 |
NN |
denotes |
μ |
T12940 |
740-747 |
NN |
denotes |
calpain |
T12939 |
726-730 |
CC |
denotes |
both |
T12938 |
723-725 |
IN |
denotes |
to |
T12937 |
716-722 |
JJ |
denotes |
common |
T12936 |
697-707 |
JJ |
denotes |
regulatory |
T12935 |
691-696 |
JJ |
denotes |
small |
T12934 |
708-715 |
NN |
denotes |
subunit |
T12933 |
687-690 |
DT |
denotes |
the |
T12932 |
678-686 |
VBG |
denotes |
encoding |
T12931 |
676-678 |
, |
denotes |
, |
T12930 |
666-671 |
NN |
denotes |
Capn4 |
T12929 |
672-676 |
NN |
denotes |
gene |
T12928 |
662-665 |
DT |
denotes |
the |
T12927 |
654-661 |
VB |
denotes |
disrupt |
T12926 |
651-653 |
TO |
denotes |
to |
T12925 |
640-645 |
RB |
denotes |
first |
T12924 |
636-639 |
VBD |
denotes |
was |
T12923 |
646-650 |
VBN |
denotes |
used |
T12922 |
631-635 |
DT |
denotes |
This |
T12921 |
630-748 |
sentence |
denotes |
This was first used to disrupt the Capn4 gene, encoding the small regulatory subunit common to both μ- and m-calpain. |
T12920 |
629-630 |
. |
denotes |
. |
T12919 |
613-620 |
NN |
denotes |
calpain |
T12918 |
621-629 |
NNS |
denotes |
isoforms |
T12917 |
602-612 |
JJ |
denotes |
individual |
T12916 |
599-601 |
IN |
denotes |
of |
T12915 |
581-592 |
JJ |
denotes |
physiologic |
T12914 |
593-598 |
NNS |
denotes |
roles |
T12913 |
577-580 |
DT |
denotes |
the |
T12912 |
569-576 |
VB |
denotes |
examine |
T12911 |
566-568 |
TO |
denotes |
to |
T12910 |
548-556 |
JJ |
denotes |
powerful |
T12909 |
557-565 |
NN |
denotes |
approach |
T12908 |
546-547 |
DT |
denotes |
a |
T12907 |
533-536 |
VBZ |
denotes |
has |
T12906 |
528-532 |
NNS |
denotes |
mice |
T12905 |
525-527 |
IN |
denotes |
in |
T12904 |
537-545 |
VBN |
denotes |
provided |
T12903 |
515-524 |
NN |
denotes |
targeting |
T12902 |
510-514 |
NN |
denotes |
Gene |
T12901 |
509-630 |
sentence |
denotes |
Gene targeting in mice has provided a powerful approach to examine the physiologic roles of individual calpain isoforms. |
T12900 |
508-509 |
. |
denotes |
. |
T12899 |
487-496 |
NN |
denotes |
substrate |
T12898 |
497-508 |
NN |
denotes |
specificity |
T12897 |
477-486 |
JJ |
denotes |
different |
T12896 |
472-476 |
VBP |
denotes |
have |
T12895 |
468-471 |
CC |
denotes |
and |
T12894 |
446-457 |
RB |
denotes |
differently |
T12893 |
443-445 |
VB |
denotes |
be |
T12892 |
439-442 |
MD |
denotes |
may |
T12891 |
458-467 |
VBN |
denotes |
regulated |
T12890 |
433-438 |
WDT |
denotes |
which |
T12889 |
424-432 |
NNS |
denotes |
isoforms |
T12888 |
416-423 |
JJ |
denotes |
unknown |
T12887 |
405-415 |
RB |
denotes |
previously |
T12886 |
402-404 |
IN |
denotes |
of |
T12885 |
395-401 |
NN |
denotes |
number |
T12884 |
393-394 |
DT |
denotes |
a |
T12883 |
390-392 |
IN |
denotes |
of |
T12882 |
380-389 |
NN |
denotes |
discovery |
T12881 |
376-379 |
DT |
denotes |
the |
T12880 |
373-375 |
IN |
denotes |
by |
T12879 |
353-360 |
RB |
denotes |
further |
T12878 |
348-352 |
VBN |
denotes |
been |
T12877 |
344-347 |
VBZ |
denotes |
has |
T12876 |
338-343 |
NN |
denotes |
story |
T12875 |
334-337 |
DT |
denotes |
the |
T12874 |
332-334 |
, |
denotes |
, |
T12873 |
321-325 |
JJ |
denotes |
past |
T12872 |
326-332 |
NN |
denotes |
decade |
T12871 |
317-320 |
DT |
denotes |
the |
T12870 |
361-372 |
VBN |
denotes |
complicated |
T12869 |
314-316 |
IN |
denotes |
In |
T12868 |
313-509 |
sentence |
denotes |
In the past decade, the story has been further complicated by the discovery of a number of previously unknown isoforms which may be differently regulated and have different substrate specificity. |
T12867 |
312-313 |
. |
denotes |
. |
T12866 |
303-312 |
NNS |
denotes |
proteases |
T12865 |
297-302 |
JJ |
denotes |
other |
T12864 |
287-296 |
VBG |
denotes |
affecting |
T12863 |
279-286 |
IN |
denotes |
without |
T12862 |
270-278 |
NNS |
denotes |
calpains |
T12861 |
266-269 |
DT |
denotes |
the |
T12860 |
254-255 |
HYPH |
denotes |
- |
T12859 |
250-254 |
RB |
denotes |
down |
T12858 |
255-265 |
VBG |
denotes |
regulating |
T12857 |
237-249 |
RB |
denotes |
specifically |
T12856 |
234-236 |
IN |
denotes |
of |
T12855 |
226-233 |
JJ |
denotes |
capable |
T12854 |
215-225 |
NNS |
denotes |
inhibitors |
T12853 |
212-214 |
IN |
denotes |
of |
T12852 |
207-211 |
NN |
denotes |
lack |
T12851 |
203-206 |
DT |
denotes |
the |
T12850 |
200-202 |
IN |
denotes |
to |
T12849 |
196-199 |
IN |
denotes |
due |
T12848 |
187-190 |
VBZ |
denotes |
has |
T12847 |
182-186 |
DT |
denotes |
this |
T12846 |
180-182 |
, |
denotes |
, |
T12845 |
176-180 |
NN |
denotes |
part |
T12844 |
170-175 |
JJ |
denotes |
large |
T12843 |
191-195 |
VBN |
denotes |
been |
T12842 |
167-169 |
IN |
denotes |
In |
T12841 |
166-313 |
sentence |
denotes |
In large part, this has been due to the lack of inhibitors capable of specifically down-regulating the calpains without affecting other proteases. |
T12840 |
165-166 |
. |
denotes |
. |
T12839 |
158-165 |
JJ |
denotes |
elusive |
T12838 |
145-148 |
VBZ |
denotes |
has |
T12837 |
137-144 |
NNS |
denotes |
enzymes |
T12836 |
133-136 |
DT |
denotes |
the |
T12835 |
130-132 |
IN |
denotes |
of |
T12834 |
120-129 |
NNS |
denotes |
functions |
T12833 |
116-119 |
CC |
denotes |
and |
T12832 |
105-115 |
NNS |
denotes |
substrates |
T12831 |
101-104 |
DT |
denotes |
the |
T12830 |
98-100 |
IN |
denotes |
of |
T12829 |
78-83 |
JJ |
denotes |
clear |
T12828 |
84-97 |
NN |
denotes |
understanding |
T12827 |
76-77 |
DT |
denotes |
a |
T12826 |
74-76 |
, |
denotes |
, |
T12825 |
71-74 |
RB |
denotes |
ago |
T12824 |
63-70 |
NNS |
denotes |
decades |
T12823 |
58-62 |
CD |
denotes |
four |
T12822 |
149-157 |
VBN |
denotes |
remained |
T12821 |
41-46 |
RB |
denotes |
first |
T12820 |
37-40 |
VBD |
denotes |
was |
T12819 |
28-36 |
NN |
denotes |
activity |
T12818 |
20-27 |
NN |
denotes |
calpain |
T12817 |
47-57 |
VBN |
denotes |
identified |
T12816 |
11-19 |
IN |
denotes |
Although |
T13307 |
2637-2639 |
IN |
denotes |
of |
T13306 |
2630-2636 |
NN |
denotes |
middle |
T13305 |
2626-2629 |
DT |
denotes |
the |
T13304 |
2621-2625 |
IN |
denotes |
into |
T13303 |
2607-2608 |
HYPH |
denotes |
- |
T13302 |
2608-2611 |
NN |
denotes |
Neo |
T13301 |
2604-2607 |
NN |
denotes |
PGK |
T13300 |
2612-2620 |
NN |
denotes |
cassette |
T13299 |
2600-2603 |
DT |
denotes |
the |
T13298 |
2597-2599 |
IN |
denotes |
of |
T13297 |
2587-2596 |
NN |
denotes |
insertion |
T13296 |
2584-2586 |
IN |
denotes |
by |
T13295 |
2570-2575 |
JJ |
denotes |
small |
T13294 |
2562-2569 |
NN |
denotes |
calpain |
T13293 |
2576-2583 |
NN |
denotes |
subunit |
T13292 |
2558-2561 |
DT |
denotes |
the |
T13291 |
2555-2557 |
IN |
denotes |
of |
T13290 |
2545-2546 |
HYPH |
denotes |
- |
T13289 |
2544-2545 |
NN |
denotes |
C |
T13288 |
2546-2554 |
NN |
denotes |
terminus |
T13287 |
2540-2543 |
DT |
denotes |
the |
T13286 |
2529-2539 |
VBG |
denotes |
disrupting |
T13285 |
2509-2519 |
NNS |
denotes |
colleagues |
T13284 |
2505-2508 |
CC |
denotes |
and |
T13283 |
2498-2504 |
NNP |
denotes |
Arthur |
T13066 |
1338-1343 |
WDT |
denotes |
which |
T13065 |
1336-1338 |
, |
denotes |
, |
T13064 |
1330-1331 |
SYM |
denotes |
- |
T13063 |
1329-1330 |
HYPH |
denotes |
/ |
T13062 |
1328-1329 |
SYM |
denotes |
- |
T13061 |
1332-1336 |
NNS |
denotes |
mice |
T13060 |
1323-1328 |
NN |
denotes |
Capn1 |
T13059 |
1382-1386 |
VBD |
denotes |
were |
T13058 |
1318-1322 |
IN |
denotes |
that |
T13057 |
1295-1305 |
JJ |
denotes |
subsequent |
T13056 |
1306-1317 |
NN |
denotes |
observation |
T13055 |
1291-1294 |
DT |
denotes |
the |
T13054 |
1288-1290 |
IN |
denotes |
by |
T13053 |
1283-1287 |
RP |
denotes |
down |
T13052 |
1269-1273 |
VBD |
denotes |
were |
T13051 |
1274-1282 |
VBN |
denotes |
narrowed |
T13050 |
1261-1268 |
NNS |
denotes |
options |
T13049 |
1255-1260 |
DT |
denotes |
These |
T13048 |
1254-1485 |
sentence |
denotes |
These options were narrowed down by the subsequent observation that Capn1-/- mice, which lack the μ-calpain catalytic subunit, were healthy and fertile, although platelet aggregation and clot retraction defects were observed [33]. |
T13047 |
1253-1254 |
. |
denotes |
. |
T13046 |
1241-1244 |
VBD |
denotes |
was |
T13045 |
1233-1240 |
NN |
denotes |
isoform |
T13044 |
1227-1232 |
JJ |
denotes |
other |
T13043 |
1223-1226 |
DT |
denotes |
the |
T13042 |
1220-1222 |
CC |
denotes |
or |
T13041 |
1245-1253 |
VBN |
denotes |
required |
T13040 |
1216-1219 |
CD |
denotes |
one |
T13039 |
1212-1215 |
CC |
denotes |
and |
T13038 |
1210-1212 |
, |
denotes |
, |
T13037 |
1201-1210 |
JJ |
denotes |
redundant |
T13036 |
1188-1189 |
HYPH |
denotes |
- |
T13035 |
1187-1188 |
NN |
denotes |
m |
T13034 |
1183-1186 |
CC |
denotes |
and |
T13033 |
1181-1182 |
HYPH |
denotes |
- |
T13032 |
1189-1196 |
NN |
denotes |
calpain |
T13031 |
1180-1181 |
NN |
denotes |
μ |
T13030 |
1178-1179 |
-RRB- |
denotes |
) |
T13029 |
1197-1200 |
VBP |
denotes |
are |
T13028 |
1177-1178 |
LS |
denotes |
4 |
T13027 |
1174-1176 |
CC |
denotes |
or |
T13026 |
1172-1174 |
, |
denotes |
, |
T13025 |
1160-1163 |
VBD |
denotes |
was |
T13024 |
1151-1152 |
HYPH |
denotes |
- |
T13023 |
1152-1159 |
NN |
denotes |
calpain |
T13022 |
1150-1151 |
NN |
denotes |
m |
T13021 |
1148-1149 |
-RRB- |
denotes |
) |
T13020 |
1164-1172 |
VBN |
denotes |
required |
T13019 |
1147-1148 |
LS |
denotes |
3 |
T13018 |
1145-1146 |
: |
denotes |
; |
T13017 |
1133-1136 |
VBD |
denotes |
was |
T13016 |
1124-1125 |
HYPH |
denotes |
- |
T13015 |
1125-1132 |
NN |
denotes |
calpain |
T13014 |
1123-1124 |
NN |
denotes |
μ |
T13013 |
1121-1122 |
-RRB- |
denotes |
) |
T13012 |
1137-1145 |
VBN |
denotes |
required |
T13011 |
1120-1121 |
LS |
denotes |
2 |
T13010 |
1118-1119 |
: |
denotes |
; |
T13009 |
1105-1109 |
VBD |
denotes |
were |
T13008 |
1096-1104 |
NNS |
denotes |
isoforms |
T13007 |
1091-1095 |
DT |
denotes |
both |
T13006 |
1089-1090 |
-RRB- |
denotes |
) |
T13005 |
1088-1089 |
LS |
denotes |
1 |
T13004 |
1086-1088 |
: |
denotes |
: |
T13003 |
1075-1086 |
NN |
denotes |
development |
T13002 |
1065-1074 |
JJ |
denotes |
embryonic |
T13001 |
1061-1064 |
IN |
denotes |
for |
T13000 |
1049-1060 |
NN |
denotes |
requirement |
T12999 |
1043-1048 |
PRP$ |
denotes |
their |
T12998 |
1033-1042 |
VBG |
denotes |
regarding |
T12997 |
1019-1032 |
NNS |
denotes |
possibilities |
T12996 |
1014-1018 |
CD |
denotes |
four |
T12995 |
1004-1013 |
VBD |
denotes |
suggested |
T12994 |
992-1003 |
RB |
denotes |
furthermore |
T12993 |
988-991 |
CC |
denotes |
and |
T12992 |
986-988 |
, |
denotes |
, |
T12991 |
978-979 |
HYPH |
denotes |
- |
T12990 |
977-978 |
NN |
denotes |
m |
T12989 |
973-976 |
CC |
denotes |
and |
T12988 |
971-972 |
HYPH |
denotes |
- |
T12987 |
970-971 |
NN |
denotes |
μ |
T12986 |
979-986 |
NN |
denotes |
calpain |
T12985 |
965-969 |
CC |
denotes |
both |
T12984 |
961-964 |
IN |
denotes |
for |
T12983 |
949-951 |
VBZ |
denotes |
is |
T12982 |
935-940 |
JJ |
denotes |
small |
T12981 |
941-948 |
NN |
denotes |
subunit |
T12980 |
931-934 |
DT |
denotes |
the |
T12979 |
952-960 |
VBN |
denotes |
required |
T12978 |
926-930 |
IN |
denotes |
that |
T12977 |
915-925 |
NN |
denotes |
hypothesis |
T12976 |
911-914 |
DT |
denotes |
the |
T12975 |
1110-1118 |
VBN |
denotes |
required |
T12974 |
901-910 |
VBD |
denotes |
supported |
T12973 |
888-900 |
NNS |
denotes |
observations |
T12972 |
882-887 |
DT |
denotes |
These |
T12971 |
881-1254 |
sentence |
denotes |
These observations supported the hypothesis that the small subunit is required for both μ- and m-calpain, and furthermore suggested four possibilities regarding their requirement for embryonic development: 1) both isoforms were required; 2) μ-calpain was required; 3) m-calpain was required, or 4) μ- and m-calpain are redundant, and one or the other isoform was required. |
T12970 |
880-881 |
. |
denotes |
. |
T12969 |
879-880 |
-RRB- |
denotes |
] |
T12968 |
876-877 |
, |
denotes |
, |
T12967 |
874-876 |
CD |
denotes |
31 |
T12966 |
877-879 |
CD |
denotes |
32 |
T12965 |
873-874 |
-LRB- |
denotes |
[ |
T12964 |
861-872 |
NN |
denotes |
development |
T12963 |
858-860 |
IN |
denotes |
of |
T12962 |
851-857 |
NNS |
denotes |
stages |
T12961 |
841-850 |
JJ |
denotes |
different |
T12960 |
838-840 |
IN |
denotes |
at |
T12959 |
831-837 |
IN |
denotes |
albeit |
T12958 |
829-831 |
, |
denotes |
, |
T15181 |
12400-12401 |
. |
denotes |
. |
T15180 |
12396-12400 |
DT |
denotes |
this |
T15179 |
12392-12395 |
IN |
denotes |
for |
T15178 |
12374-12385 |
JJ |
denotes |
mechanistic |
T15177 |
12386-12391 |
NN |
denotes |
basis |
T15176 |
12372-12373 |
DT |
denotes |
a |
T15175 |
12360-12364 |
MD |
denotes |
will |
T15174 |
12352-12359 |
NNS |
denotes |
studies |
T15173 |
12345-12351 |
JJ |
denotes |
future |
T15172 |
12365-12371 |
VB |
denotes |
reveal |
T15171 |
12337-12344 |
RB |
denotes |
Perhaps |
T15170 |
12336-12401 |
sentence |
denotes |
Perhaps future studies will reveal a mechanistic basis for this. |
T15169 |
12335-12336 |
. |
denotes |
. |
T15160 |
12259-12266 |
VBP |
denotes |
suggest |
T15159 |
12246-12258 |
NNS |
denotes |
observations |
T15158 |
12240-12245 |
DT |
denotes |
These |
T15157 |
12239-12336 |
sentence |
denotes |
These observations suggest a developmental advantage associated with reduced calpain expression. |
T15156 |
12238-12239 |
. |
denotes |
. |
T15155 |
12237-12238 |
-RRB- |
denotes |
] |
T15154 |
12235-12237 |
CD |
denotes |
32 |
T15153 |
12234-12235 |
-LRB- |
denotes |
[ |
T15152 |
12212-12216 |
JJ |
denotes |
null |
T15151 |
12201-12211 |
JJ |
denotes |
homozygous |
T15150 |
12217-12224 |
NN |
denotes |
progeny |
T15149 |
12198-12200 |
DT |
denotes |
no |
T15148 |
12225-12233 |
VBN |
denotes |
observed |
T15147 |
12193-12197 |
IN |
denotes |
with |
T15146 |
12191-12193 |
, |
denotes |
, |
T15145 |
12179-12191 |
JJ |
denotes |
heterozygous |
T15144 |
12174-12178 |
VBD |
denotes |
were |
T15143 |
12172-12173 |
NN |
denotes |
% |
T15142 |
12168-12172 |
CD |
denotes |
77.5 |
T15141 |
12164-12167 |
CC |
denotes |
and |
T15140 |
12158-12159 |
HYPH |
denotes |
- |
T15139 |
12159-12163 |
NN |
denotes |
type |
T15138 |
12154-12158 |
JJ |
denotes |
wild |
T15168 |
12317-12324 |
NN |
denotes |
calpain |
T15167 |
12325-12335 |
NN |
denotes |
expression |
T15166 |
12309-12316 |
VBN |
denotes |
reduced |
T15165 |
12304-12308 |
IN |
denotes |
with |
T15164 |
12293-12303 |
VBN |
denotes |
associated |
T15163 |
12269-12282 |
JJ |
denotes |
developmental |
T15162 |
12283-12292 |
NN |
denotes |
advantage |
R2370 |
T12875 |
T12876 |
det |
the,story |
R2373 |
T12816 |
T12817 |
mark |
Although,identified |
R2374 |
T12876 |
T12870 |
nsubjpass |
story,complicated |
R2375 |
T12877 |
T12870 |
aux |
has,complicated |
R2376 |
T12817 |
T12822 |
advcl |
identified,remained |
R2377 |
T12818 |
T12819 |
compound |
calpain,activity |
R2378 |
T12878 |
T12870 |
auxpass |
been,complicated |
R2379 |
T12819 |
T12817 |
nsubjpass |
activity,identified |
R2380 |
T12820 |
T12817 |
auxpass |
was,identified |
R2381 |
T12821 |
T12817 |
advmod |
first,identified |
R2382 |
T12879 |
T12870 |
advmod |
further,complicated |
R2383 |
T12823 |
T12824 |
nummod |
four,decades |
R2384 |
T12880 |
T12870 |
agent |
by,complicated |
R2385 |
T12824 |
T12825 |
npadvmod |
decades,ago |
R2386 |
T12825 |
T12817 |
advmod |
ago,identified |
R2387 |
T12826 |
T12822 |
punct |
", ",remained |
R2388 |
T12881 |
T12882 |
det |
the,discovery |
R2389 |
T12827 |
T12828 |
det |
a,understanding |
R2390 |
T12828 |
T12822 |
nsubj |
understanding,remained |
R2391 |
T12882 |
T12880 |
pobj |
discovery,by |
R2392 |
T12829 |
T12828 |
amod |
clear,understanding |
R2393 |
T12830 |
T12828 |
prep |
of,understanding |
R2394 |
T12831 |
T12832 |
det |
the,substrates |
R2395 |
T12883 |
T12882 |
prep |
of,discovery |
R2396 |
T12884 |
T12885 |
det |
a,number |
R2397 |
T12832 |
T12830 |
pobj |
substrates,of |
R2398 |
T12885 |
T12883 |
pobj |
number,of |
R2399 |
T12833 |
T12832 |
cc |
and,substrates |
R2400 |
T12834 |
T12832 |
conj |
functions,substrates |
R2401 |
T12886 |
T12885 |
prep |
of,number |
R2402 |
T12835 |
T12832 |
prep |
of,substrates |
R2403 |
T12836 |
T12837 |
det |
the,enzymes |
R2404 |
T12887 |
T12888 |
advmod |
previously,unknown |
R2405 |
T12837 |
T12835 |
pobj |
enzymes,of |
R2406 |
T12838 |
T12822 |
aux |
has,remained |
R2407 |
T12839 |
T12822 |
acomp |
elusive,remained |
R2408 |
T12840 |
T12822 |
punct |
.,remained |
R2409 |
T12842 |
T12843 |
prep |
In,been |
R2410 |
T12844 |
T12845 |
amod |
large,part |
R2411 |
T12845 |
T12842 |
pobj |
part,In |
R2412 |
T12846 |
T12843 |
punct |
", ",been |
R2413 |
T12847 |
T12843 |
nsubj |
this,been |
R2414 |
T12848 |
T12843 |
aux |
has,been |
R2415 |
T12849 |
T12843 |
prep |
due,been |
R2416 |
T12850 |
T12849 |
pcomp |
to,due |
R2417 |
T12888 |
T12889 |
amod |
unknown,isoforms |
R2418 |
T12851 |
T12852 |
det |
the,lack |
R2419 |
T12852 |
T12849 |
pobj |
lack,due |
R2420 |
T12853 |
T12852 |
prep |
of,lack |
R2421 |
T12854 |
T12853 |
pobj |
inhibitors,of |
R2422 |
T12855 |
T12854 |
amod |
capable,inhibitors |
R2423 |
T12856 |
T12855 |
prep |
of,capable |
R2424 |
T12889 |
T12886 |
pobj |
isoforms,of |
R2425 |
T12857 |
T12858 |
advmod |
specifically,regulating |
R2426 |
T12858 |
T12856 |
pcomp |
regulating,of |
R2427 |
T12859 |
T12858 |
advmod |
down,regulating |
R2428 |
T12890 |
T12891 |
dep |
which,regulated |
R2429 |
T12860 |
T12858 |
punct |
-,regulating |
R2430 |
T12861 |
T12862 |
det |
the,calpains |
R2431 |
T12862 |
T12858 |
dobj |
calpains,regulating |
R2432 |
T12891 |
T12889 |
relcl |
regulated,isoforms |
R2433 |
T12863 |
T12858 |
prep |
without,regulating |
R2434 |
T12864 |
T12863 |
pcomp |
affecting,without |
R2435 |
T12865 |
T12866 |
amod |
other,proteases |
R2436 |
T12892 |
T12891 |
aux |
may,regulated |
R2437 |
T12866 |
T12864 |
dobj |
proteases,affecting |
R2438 |
T12867 |
T12843 |
punct |
.,been |
R2439 |
T12893 |
T12891 |
auxpass |
be,regulated |
R2440 |
T12869 |
T12870 |
prep |
In,complicated |
R2441 |
T12894 |
T12891 |
advmod |
differently,regulated |
R2442 |
T12871 |
T12872 |
det |
the,decade |
R2443 |
T12872 |
T12869 |
pobj |
decade,In |
R2444 |
T12873 |
T12872 |
amod |
past,decade |
R2445 |
T12895 |
T12891 |
cc |
and,regulated |
R2446 |
T12874 |
T12870 |
punct |
", ",complicated |
R2447 |
T12896 |
T12891 |
conj |
have,regulated |
R2448 |
T12897 |
T12898 |
amod |
different,specificity |
R2449 |
T12898 |
T12896 |
dobj |
specificity,have |
R2450 |
T12899 |
T12898 |
compound |
substrate,specificity |
R2451 |
T12981 |
T12979 |
nsubjpass |
subunit,required |
R2452 |
T12900 |
T12870 |
punct |
.,complicated |
R2453 |
T12982 |
T12981 |
amod |
small,subunit |
R2454 |
T12983 |
T12979 |
auxpass |
is,required |
R2455 |
T12902 |
T12903 |
compound |
Gene,targeting |
R2456 |
T12984 |
T12979 |
prep |
for,required |
R2457 |
T12985 |
T12986 |
preconj |
both,calpain |
R2458 |
T12903 |
T12904 |
nsubj |
targeting,provided |
R2459 |
T12905 |
T12903 |
prep |
in,targeting |
R2460 |
T12986 |
T12984 |
pobj |
calpain,for |
R2461 |
T12987 |
T12986 |
nmod |
μ,calpain |
R2462 |
T12988 |
T12987 |
punct |
-,μ |
R2463 |
T12989 |
T12987 |
cc |
and,μ |
R2464 |
T12990 |
T12987 |
conj |
m,μ |
R2465 |
T12991 |
T12986 |
punct |
-,calpain |
R2466 |
T12992 |
T12974 |
punct |
", ",supported |
R2467 |
T12993 |
T12974 |
cc |
and,supported |
R2468 |
T12994 |
T12995 |
advmod |
furthermore,suggested |
R2469 |
T12906 |
T12905 |
pobj |
mice,in |
R2470 |
T12995 |
T12974 |
conj |
suggested,supported |
R2471 |
T12996 |
T12997 |
nummod |
four,possibilities |
R2472 |
T12997 |
T12995 |
dobj |
possibilities,suggested |
R2473 |
T12907 |
T12904 |
aux |
has,provided |
R2474 |
T12998 |
T12997 |
prep |
regarding,possibilities |
R2475 |
T12999 |
T13000 |
poss |
their,requirement |
R2476 |
T13000 |
T12998 |
pobj |
requirement,regarding |
R2477 |
T12908 |
T12909 |
det |
a,approach |
R2478 |
T13001 |
T13000 |
prep |
for,requirement |
R2479 |
T13002 |
T13003 |
amod |
embryonic,development |
R2480 |
T13003 |
T13001 |
pobj |
development,for |
R2481 |
T12909 |
T12904 |
dobj |
approach,provided |
R2482 |
T13004 |
T12975 |
punct |
: ,required |
R2483 |
T13005 |
T12975 |
meta |
1,required |
R2484 |
T13006 |
T13005 |
punct |
),1 |
R2485 |
T12910 |
T12909 |
amod |
powerful,approach |
R2486 |
T13007 |
T13008 |
det |
both,isoforms |
R2487 |
T13008 |
T12975 |
nsubjpass |
isoforms,required |
R2488 |
T12911 |
T12912 |
aux |
to,examine |
R2489 |
T13009 |
T12975 |
auxpass |
were,required |
R2490 |
T13010 |
T12975 |
punct |
;,required |
R2491 |
T13011 |
T13012 |
meta |
2,required |
R2492 |
T13012 |
T12975 |
conj |
required,required |
R2493 |
T13013 |
T13011 |
punct |
),2 |
R2494 |
T12912 |
T12909 |
advcl |
examine,approach |
R2495 |
T13014 |
T13015 |
compound |
μ,calpain |
R2496 |
T13015 |
T13012 |
nsubjpass |
calpain,required |
R2497 |
T13016 |
T13015 |
punct |
-,calpain |
R2498 |
T12913 |
T12914 |
det |
the,roles |
R2499 |
T13017 |
T13012 |
auxpass |
was,required |
R2500 |
T13018 |
T13012 |
punct |
;,required |
R2501 |
T13019 |
T13020 |
meta |
3,required |
R2502 |
T12914 |
T12912 |
dobj |
roles,examine |
R2503 |
T13020 |
T13012 |
conj |
required,required |
R2504 |
T13021 |
T13019 |
punct |
),3 |
R2505 |
T13022 |
T13023 |
compound |
m,calpain |
R2506 |
T12915 |
T12914 |
amod |
physiologic,roles |
R2507 |
T13023 |
T13020 |
nsubjpass |
calpain,required |
R2508 |
T13024 |
T13023 |
punct |
-,calpain |
R2509 |
T13025 |
T13020 |
auxpass |
was,required |
R2510 |
T12916 |
T12914 |
prep |
of,roles |
R2511 |
T13026 |
T13020 |
punct |
", ",required |
R2512 |
T13027 |
T13020 |
cc |
or,required |
R2513 |
T13028 |
T13029 |
meta |
4,are |
R2514 |
T13029 |
T13020 |
conj |
are,required |
R2515 |
T13030 |
T13028 |
punct |
),4 |
R2516 |
T13031 |
T13032 |
nmod |
μ,calpain |
R2517 |
T13032 |
T13029 |
nsubj |
calpain,are |
R2518 |
T13033 |
T13031 |
punct |
-,μ |
R2519 |
T12917 |
T12918 |
amod |
individual,isoforms |
R2520 |
T13034 |
T13031 |
cc |
and,μ |
R2521 |
T13035 |
T13031 |
conj |
m,μ |
R2522 |
T13036 |
T13032 |
punct |
-,calpain |
R2523 |
T12918 |
T12916 |
pobj |
isoforms,of |
R2524 |
T13037 |
T13029 |
acomp |
redundant,are |
R2525 |
T13038 |
T13029 |
punct |
", ",are |
R2526 |
T13039 |
T13029 |
cc |
and,are |
R2527 |
T12919 |
T12918 |
compound |
calpain,isoforms |
R2528 |
T13040 |
T13041 |
nsubjpass |
one,required |
R2529 |
T13041 |
T13029 |
conj |
required,are |
R2530 |
T13042 |
T13040 |
cc |
or,one |
R2531 |
T12920 |
T12904 |
punct |
.,provided |
R2532 |
T13043 |
T13044 |
det |
the,other |
R2533 |
T13044 |
T13045 |
nmod |
other,isoform |
R2534 |
T13045 |
T13040 |
conj |
isoform,one |
R2535 |
T12922 |
T12923 |
nsubjpass |
This,used |
R2536 |
T13046 |
T13041 |
auxpass |
was,required |
R2537 |
T13047 |
T12975 |
punct |
.,required |
R2538 |
T13049 |
T13050 |
det |
These,options |
R2539 |
T12924 |
T12923 |
auxpass |
was,used |
R2540 |
T13050 |
T13051 |
nsubjpass |
options,narrowed |
R2541 |
T13052 |
T13051 |
auxpass |
were,narrowed |
R2542 |
T13053 |
T13051 |
prt |
down,narrowed |
R2543 |
T13054 |
T13051 |
agent |
by,narrowed |
R2544 |
T12925 |
T12923 |
advmod |
first,used |
R2545 |
T13055 |
T13056 |
det |
the,observation |
R2546 |
T13056 |
T13054 |
pobj |
observation,by |
R2547 |
T13057 |
T13056 |
amod |
subsequent,observation |
R2548 |
T13058 |
T13059 |
mark |
that,were |
R2549 |
T12926 |
T12927 |
aux |
to,disrupt |
R2550 |
T12927 |
T12923 |
advcl |
disrupt,used |
R2551 |
T13059 |
T13056 |
acl |
were,observation |
R2552 |
T12928 |
T12929 |
det |
the,gene |
R2553 |
T13060 |
T13061 |
nmod |
Capn1,mice |
R2554 |
T13061 |
T13059 |
nsubj |
mice,were |
R2555 |
T13062 |
T13060 |
punct |
-,Capn1 |
R2556 |
T13063 |
T13060 |
punct |
/,Capn1 |
R2557 |
T12929 |
T12927 |
dobj |
gene,disrupt |
R2558 |
T13064 |
T13060 |
punct |
-,Capn1 |
R2559 |
T13065 |
T13061 |
punct |
", ",mice |
R2560 |
T12930 |
T12929 |
compound |
Capn4,gene |
R2561 |
T13066 |
T13067 |
dep |
which,lack |
R2562 |
T13067 |
T13061 |
relcl |
lack,mice |
R2563 |
T13068 |
T13069 |
det |
the,subunit |
R2564 |
T13069 |
T13067 |
dobj |
subunit,lack |
R2565 |
T13070 |
T13071 |
nmod |
μ,calpain |
R2566 |
T13071 |
T13069 |
nmod |
calpain,subunit |
R2567 |
T13072 |
T13071 |
punct |
-,calpain |
R2568 |
T13073 |
T13069 |
amod |
catalytic,subunit |
R2569 |
T13074 |
T13059 |
punct |
", ",were |
R2570 |
T13075 |
T13059 |
acomp |
healthy,were |
R2571 |
T13076 |
T13075 |
cc |
and,healthy |
R2572 |
T12931 |
T12923 |
punct |
", ",used |
R2573 |
T13077 |
T13075 |
conj |
fertile,healthy |
R2574 |
T13078 |
T13059 |
punct |
", ",were |
R2575 |
T13079 |
T13080 |
mark |
although,observed |
R2576 |
T12932 |
T12923 |
advcl |
encoding,used |
R2577 |
T13080 |
T13059 |
advcl |
observed,were |
R2578 |
T13081 |
T13082 |
nmod |
platelet,aggregation |
R2579 |
T12933 |
T12934 |
det |
the,subunit |
R2580 |
T13082 |
T13083 |
nmod |
aggregation,defects |
R2581 |
T13083 |
T13080 |
nsubjpass |
defects,observed |
R2582 |
T13084 |
T13082 |
cc |
and,aggregation |
R2583 |
T13085 |
T13086 |
compound |
clot,retraction |
R2584 |
T12934 |
T12932 |
dobj |
subunit,encoding |
R2585 |
T13086 |
T13082 |
conj |
retraction,aggregation |
R2586 |
T12935 |
T12934 |
amod |
small,subunit |
R2587 |
T12936 |
T12934 |
amod |
regulatory,subunit |
R2588 |
T12937 |
T12934 |
amod |
common,subunit |
R2589 |
T13087 |
T13080 |
auxpass |
were,observed |
R2590 |
T12938 |
T12937 |
prep |
to,common |
R2591 |
T13088 |
T13089 |
punct |
[,33 |
R2592 |
T13089 |
T13059 |
parataxis |
33,were |
R2593 |
T13090 |
T13089 |
punct |
],33 |
R2594 |
T12939 |
T12940 |
preconj |
both,calpain |
R2595 |
T13091 |
T13051 |
punct |
.,narrowed |
R2596 |
T12940 |
T12938 |
pobj |
calpain,to |
R2597 |
T13093 |
T13094 |
prep |
At,left |
R2598 |
T13095 |
T13096 |
det |
that,point |
R2599 |
T12941 |
T12940 |
nmod |
μ,calpain |
R2600 |
T13096 |
T13093 |
pobj |
point,At |
R2601 |
T13097 |
T13094 |
punct |
", ",left |
R2602 |
T13098 |
T13094 |
nsubjpass |
we,left |
R2603 |
T12942 |
T12941 |
punct |
-,μ |
R2604 |
T13099 |
T13094 |
auxpass |
were,left |
R2605 |
T13100 |
T13094 |
prep |
with,left |
R2606 |
T13101 |
T13102 |
det |
the,possibilities |
R2607 |
T12943 |
T12941 |
cc |
and,μ |
R2608 |
T13102 |
T13100 |
pobj |
possibilities,with |
R2609 |
T13103 |
T13102 |
amod |
last,possibilities |
R2610 |
T13104 |
T13102 |
nummod |
two,possibilities |
R2611 |
T12944 |
T12941 |
conj |
m,μ |
R2612 |
T13105 |
T13106 |
mark |
that,required |
R2613 |
T13106 |
T13102 |
advcl |
required,possibilities |
R2614 |
T13107 |
T13108 |
det |
either,calpain |
R2615 |
T13108 |
T13106 |
nsubjpass |
calpain,required |
R2616 |
T13109 |
T13108 |
compound |
m,calpain |
R2617 |
T13110 |
T13108 |
punct |
-,calpain |
R2618 |
T13111 |
T13106 |
auxpass |
was,required |
R2619 |
T13112 |
T13106 |
advmod |
specifically,required |
R2620 |
T13113 |
T13106 |
prep |
during,required |
R2621 |
T12945 |
T12940 |
punct |
-,calpain |
R2622 |
T13114 |
T13113 |
pobj |
embryogenesis,during |
R2623 |
T13115 |
T13106 |
punct |
", ",required |
R2624 |
T13116 |
T13106 |
cc |
or,required |
R2625 |
T13117 |
T13118 |
mark |
that,was |
R2626 |
T12946 |
T12923 |
punct |
.,used |
R2627 |
T13118 |
T13106 |
conj |
was,required |
R2628 |
T13119 |
T13120 |
det |
either,isoform |
R2629 |
T13120 |
T13118 |
nsubj |
isoform,was |
R2630 |
T13121 |
T13120 |
advmod |
alone,isoform |
R2631 |
T13122 |
T13118 |
acomp |
sufficient,was |
R2632 |
T12948 |
T12949 |
nummod |
Two,laboratories |
R2633 |
T13123 |
T13122 |
prep |
for,sufficient |
R2634 |
T13124 |
T13123 |
pcomp |
sustaining,for |
R2635 |
T13125 |
T13126 |
amod |
embryonic,viability |
R2636 |
T12949 |
T12951 |
nsubj |
laboratories,observed |
R2637 |
T13126 |
T13124 |
dobj |
viability,sustaining |
R2638 |
T13127 |
T13094 |
punct |
.,left |
R2639 |
T13129 |
T13130 |
nsubj |
We,report |
R2640 |
T12950 |
T12949 |
amod |
independent,laboratories |
R2641 |
T13131 |
T13130 |
advmod |
here,report |
R2642 |
T13132 |
T13133 |
mark |
that,die |
R2643 |
T12952 |
T12953 |
amod |
embryonic,lethality |
R2644 |
T13133 |
T13130 |
ccomp |
die,report |
R2645 |
T13134 |
T13135 |
nmod |
Capn2,mice |
R2646 |
T13135 |
T13133 |
nsubj |
mice,die |
R2647 |
T13136 |
T13134 |
punct |
-,Capn2 |
R2648 |
T13137 |
T13134 |
punct |
/,Capn2 |
R2649 |
T13138 |
T13134 |
punct |
-,Capn2 |
R2650 |
T12953 |
T12951 |
dobj |
lethality,observed |
R2651 |
T13139 |
T13135 |
punct |
", ",mice |
R2652 |
T13140 |
T13141 |
dep |
which,lack |
R2653 |
T13141 |
T13135 |
relcl |
lack,mice |
R2654 |
T12954 |
T12951 |
prep |
in,observed |
R2655 |
T13142 |
T13143 |
det |
the,subunit |
R2656 |
T13143 |
T13141 |
dobj |
subunit,lack |
R2657 |
T13144 |
T13145 |
nmod |
m,calpain |
R2658 |
T12955 |
T12956 |
compound |
Capn4,mice |
R2659 |
T13145 |
T13143 |
nmod |
calpain,subunit |
R2660 |
T13146 |
T13145 |
punct |
-,calpain |
R2661 |
T13147 |
T13143 |
amod |
catalytic,subunit |
R2662 |
T12956 |
T12954 |
pobj |
mice,in |
R2663 |
T13148 |
T13133 |
punct |
", ",die |
R2664 |
T13149 |
T13133 |
prep |
at,die |
R2665 |
T13150 |
T13151 |
det |
the,stage |
R2666 |
T12957 |
T12956 |
compound |
knockout,mice |
R2667 |
T13151 |
T13149 |
pobj |
stage,at |
R2668 |
T13152 |
T13151 |
compound |
preimplantation,stage |
R2669 |
T13153 |
T13151 |
prep |
of,stage |
R2670 |
T13154 |
T13153 |
pobj |
development,of |
R2671 |
T13155 |
T13130 |
punct |
.,report |
R2672 |
T12958 |
T12951 |
punct |
", ",observed |
R2673 |
T13157 |
T13158 |
det |
This,observation |
R2674 |
T12959 |
T12960 |
dep |
albeit,at |
R2675 |
T12960 |
T12951 |
prep |
at,observed |
R2676 |
T13158 |
T13159 |
nsubj |
observation,allows |
R2677 |
T12961 |
T12962 |
amod |
different,stages |
R2678 |
T13160 |
T13161 |
nsubj |
us,conclude |
R2679 |
T13161 |
T13159 |
ccomp |
conclude,allows |
R2680 |
T12962 |
T12960 |
pobj |
stages,at |
R2681 |
T13162 |
T13161 |
aux |
to,conclude |
R2682 |
T13163 |
T13161 |
advmod |
now,conclude |
R2683 |
T13164 |
T13165 |
mark |
that,required |
R2684 |
T12963 |
T12962 |
prep |
of,stages |
R2685 |
T13165 |
T13161 |
ccomp |
required,conclude |
R2686 |
T13166 |
T13167 |
compound |
m,calpain |
R2687 |
T12964 |
T12963 |
pobj |
development,of |
R2688 |
T13167 |
T13165 |
nsubjpass |
calpain,required |
R2689 |
T13168 |
T13167 |
punct |
-,calpain |
R2690 |
T13169 |
T13165 |
auxpass |
is,required |
R2691 |
T12965 |
T12966 |
punct |
[,32 |
R2692 |
T13170 |
T13165 |
advmod |
specifically,required |
R2693 |
T13171 |
T13165 |
prep |
during,required |
R2694 |
T13172 |
T13171 |
pobj |
embryogenesis,during |
R2695 |
T12966 |
T12951 |
parataxis |
32,observed |
R2696 |
T13173 |
T13159 |
punct |
.,allows |
R2697 |
T12967 |
T12966 |
nummod |
31,32 |
R2698 |
T13175 |
T13176 |
mark |
Since,expected |
R2699 |
T13176 |
T13185 |
advcl |
expected,is |
R2700 |
T13177 |
T13178 |
amod |
homozygous,disruption |
R2701 |
T12968 |
T12966 |
punct |
",",32 |
R2702 |
T13178 |
T13176 |
nsubjpass |
disruption,expected |
R2703 |
T12969 |
T12966 |
punct |
],32 |
R2704 |
T13179 |
T13178 |
prep |
of,disruption |
R2705 |
T13180 |
T13181 |
det |
the,gene |
R2706 |
T13181 |
T13179 |
pobj |
gene,of |
R2707 |
T12970 |
T12951 |
punct |
.,observed |
R2708 |
T13182 |
T13181 |
compound |
Capn4,gene |
R2709 |
T13183 |
T13176 |
auxpass |
was,expected |
R2710 |
T12972 |
T12973 |
det |
These,observations |
R2711 |
T13184 |
T13176 |
advmod |
also,expected |
R2712 |
T13186 |
T13187 |
aux |
to,abolish |
R2713 |
T13187 |
T13176 |
xcomp |
abolish,expected |
R2714 |
T12973 |
T12974 |
nsubj |
observations,supported |
R2715 |
T13188 |
T13189 |
compound |
m,calpain |
R2716 |
T13189 |
T13191 |
compound |
calpain,activity |
R2717 |
T13190 |
T13189 |
punct |
-,calpain |
R2718 |
T12974 |
T12975 |
ccomp |
supported,required |
R2719 |
T13191 |
T13187 |
dobj |
activity,abolish |
R2720 |
T13192 |
T13185 |
punct |
", ",is |
R2721 |
T12976 |
T12977 |
det |
the,hypothesis |
R2722 |
T13193 |
T13194 |
det |
this,result |
R2723 |
T13194 |
T13185 |
nsubj |
result,is |
R2724 |
T12977 |
T12974 |
dobj |
hypothesis,supported |
R2725 |
T13195 |
T13185 |
prep |
in,is |
R2726 |
T12978 |
T12979 |
mark |
that,required |
R2727 |
T13196 |
T13195 |
pobj |
agreement,in |
R2728 |
T13197 |
T13196 |
prep |
with,agreement |
R2729 |
T13198 |
T13199 |
det |
the,phenotype |
R2730 |
T13199 |
T13197 |
pobj |
phenotype,with |
R2731 |
T13200 |
T13199 |
acl |
presented,phenotype |
R2732 |
T12979 |
T12977 |
acl |
required,hypothesis |
R2733 |
T13201 |
T13200 |
agent |
by,presented |
R2734 |
T13202 |
T13201 |
pobj |
one,by |
R2735 |
T13203 |
T13202 |
prep |
of,one |
R2736 |
T12980 |
T12981 |
det |
the,subunit |
R2737 |
T13204 |
T13205 |
det |
the,lines |
R2738 |
T13205 |
T13203 |
pobj |
lines,of |
R2739 |
T13299 |
T13300 |
det |
the,cassette |
R2740 |
T13206 |
T13205 |
nummod |
two,lines |
R2741 |
T13207 |
T13208 |
npadvmod |
Capn4,targeted |
R2742 |
T13208 |
T13205 |
amod |
targeted,lines |
R2743 |
T13209 |
T13210 |
prep |
in,observed |
R2744 |
T13300 |
T13298 |
pobj |
cassette,of |
R2745 |
T13210 |
T13205 |
relcl |
observed,lines |
R2746 |
T13211 |
T13209 |
pobj |
which,in |
R2747 |
T13212 |
T13213 |
compound |
preimplantation,lethality |
R2748 |
T13301 |
T13302 |
compound |
PGK,Neo |
R2749 |
T13213 |
T13210 |
nsubjpass |
lethality,observed |
R2750 |
T13214 |
T13213 |
prep |
of,lethality |
R2751 |
T13215 |
T13216 |
amod |
null,embryos |
R2752 |
T13216 |
T13214 |
pobj |
embryos,of |
R2753 |
T13302 |
T13300 |
compound |
Neo,cassette |
R2754 |
T13217 |
T13210 |
auxpass |
was,observed |
R2755 |
T13218 |
T13210 |
advmod |
also,observed |
R2756 |
T13219 |
T13220 |
punct |
[,32 |
R2757 |
T13303 |
T13302 |
punct |
-,Neo |
R2758 |
T13304 |
T13297 |
prep |
into,insertion |
R2759 |
T13220 |
T13210 |
parataxis |
32,observed |
R2760 |
T13305 |
T13306 |
det |
the,middle |
R2761 |
T13221 |
T13220 |
punct |
],32 |
R2762 |
T13222 |
T13185 |
punct |
.,is |
R2763 |
T13306 |
T13304 |
pobj |
middle,into |
R2764 |
T13224 |
T13225 |
det |
The,survival |
R2765 |
T13307 |
T13306 |
prep |
of,middle |
R2766 |
T13225 |
T13226 |
nsubj |
survival,is |
R2767 |
T13308 |
T13307 |
pobj |
exon,of |
R2768 |
T13227 |
T13225 |
prep |
of,survival |
R2769 |
T13228 |
T13229 |
nmod |
Capn4,embryos |
R2770 |
T13229 |
T13227 |
pobj |
embryos,of |
R2771 |
T13230 |
T13228 |
punct |
-,Capn4 |
R2772 |
T13231 |
T13228 |
punct |
/,Capn4 |
R2773 |
T13232 |
T13228 |
punct |
-,Capn4 |
R2774 |
T13233 |
T13225 |
prep |
from,survival |
R2775 |
T13309 |
T13308 |
nummod |
9,exon |
R2776 |
T13234 |
T13235 |
det |
the,line |
R2777 |
T13235 |
T13233 |
pobj |
line,from |
R2778 |
T13236 |
T13235 |
amod |
original,line |
R2779 |
T13310 |
T13297 |
punct |
", ",insertion |
R2780 |
T13237 |
T13235 |
amod |
targeted,line |
R2781 |
T13238 |
T13235 |
acl |
reported,line |
R2782 |
T13311 |
T13312 |
dep |
which,caused |
R2783 |
T13239 |
T13238 |
agent |
by,reported |
R2784 |
T13240 |
T13239 |
pobj |
Arthur,by |
R2785 |
T13241 |
T13240 |
cc |
and,Arthur |
R2786 |
T13312 |
T13297 |
relcl |
caused,insertion |
R2787 |
T13242 |
T13240 |
conj |
colleagues,Arthur |
R2788 |
T13313 |
T13312 |
dobj |
truncation,caused |
R2789 |
T13314 |
T13313 |
prep |
of,truncation |
R2790 |
T13243 |
T13233 |
prep |
to,from |
R2791 |
T13244 |
T13243 |
pobj |
mid-gestation,to |
R2792 |
T13315 |
T13316 |
det |
the,protein |
R2793 |
T13245 |
T13246 |
advmod |
more,difficult |
R2794 |
T13246 |
T13226 |
acomp |
difficult,is |
R2795 |
T13316 |
T13314 |
pobj |
protein,of |
R2796 |
T13247 |
T13248 |
aux |
to,reconcile |
R2797 |
T13248 |
T13246 |
advcl |
reconcile,difficult |
R2798 |
T13249 |
T13250 |
punct |
[,31 |
R2799 |
T13317 |
T13318 |
punct |
[,31 |
R2800 |
T13250 |
T13226 |
parataxis |
31,is |
R2801 |
T13251 |
T13250 |
punct |
],31 |
R2802 |
T13318 |
T13312 |
parataxis |
31,caused |
R2803 |
T13252 |
T13226 |
punct |
.,is |
R2804 |
T13254 |
T13255 |
prep |
In,seems |
R2805 |
T13319 |
T13318 |
punct |
],31 |
R2806 |
T13256 |
T13254 |
pobj |
retrospect,In |
R2807 |
T13320 |
T13280 |
punct |
.,involved |
R2808 |
T13257 |
T13255 |
punct |
", ",seems |
R2809 |
T13258 |
T13255 |
nsubj |
it,seems |
R2810 |
T13259 |
T13255 |
oprd |
likely,seems |
R2811 |
T13322 |
T13323 |
det |
This,strategy |
R2812 |
T13260 |
T13261 |
mark |
that,represents |
R2813 |
T13261 |
T13255 |
ccomp |
represents,seems |
R2814 |
T13262 |
T13263 |
det |
the,line |
R2815 |
T13263 |
T13261 |
nsubj |
line,represents |
R2816 |
T13323 |
T13324 |
nsubjpass |
strategy,based |
R2817 |
T13264 |
T13263 |
amod |
latter,line |
R2818 |
T13265 |
T13266 |
det |
a,state |
R2819 |
T13325 |
T13324 |
auxpass |
was,based |
R2820 |
T13266 |
T13261 |
dobj |
state,represents |
R2821 |
T13267 |
T13266 |
amod |
hypomorphic,state |
R2822 |
T13268 |
T13266 |
punct |
", ",state |
R2823 |
T13269 |
T13270 |
advmod |
rather,than |
R2824 |
T13270 |
T13266 |
cc |
than,state |
R2825 |
T13271 |
T13272 |
det |
a,null |
R2826 |
T13272 |
T13266 |
conj |
null,state |
R2827 |
T13273 |
T13272 |
amod |
true,null |
R2828 |
T13274 |
T13255 |
punct |
.,seems |
R2829 |
T13326 |
T13324 |
prep |
upon,based |
R2830 |
T13276 |
T13277 |
det |
The,strategy |
R2831 |
T13277 |
T13280 |
nsubj |
strategy,involved |
R2832 |
T13327 |
T13328 |
amod |
previous,studies |
R2833 |
T13278 |
T13277 |
compound |
Capn4,strategy |
R2834 |
T13279 |
T13277 |
compound |
targeting,strategy |
R2835 |
T13328 |
T13326 |
pobj |
studies,upon |
R2836 |
T13281 |
T13277 |
acl |
employed,strategy |
R2837 |
T13282 |
T13281 |
agent |
by,employed |
R2838 |
T13283 |
T13282 |
pobj |
Arthur,by |
R2839 |
T13284 |
T13283 |
cc |
and,Arthur |
R2840 |
T13285 |
T13283 |
conj |
colleagues,Arthur |
R2841 |
T13329 |
T13330 |
compound |
structure,function |
R2842 |
T13286 |
T13280 |
xcomp |
disrupting,involved |
R2843 |
T13287 |
T13288 |
det |
the,terminus |
R2844 |
T13288 |
T13286 |
dobj |
terminus,disrupting |
R2845 |
T13330 |
T13328 |
compound |
function,studies |
R2846 |
T13289 |
T13288 |
compound |
C,terminus |
R2847 |
T13290 |
T13288 |
punct |
-,terminus |
R2848 |
T13291 |
T13288 |
prep |
of,terminus |
R2849 |
T13292 |
T13293 |
det |
the,subunit |
R2850 |
T13331 |
T13330 |
punct |
/,function |
R2851 |
T13293 |
T13291 |
pobj |
subunit,of |
R2852 |
T13294 |
T13293 |
nmod |
calpain,subunit |
R2853 |
T13332 |
T13328 |
acl |
showing,studies |
R2854 |
T13295 |
T13293 |
amod |
small,subunit |
R2855 |
T13296 |
T13286 |
agent |
by,disrupting |
R2856 |
T13297 |
T13296 |
pobj |
insertion,by |
R2857 |
T13333 |
T13334 |
mark |
that,abolished |
R2858 |
T13298 |
T13297 |
prep |
of,insertion |
R2859 |
T13334 |
T13332 |
ccomp |
abolished,showing |
R2860 |
T13335 |
T13334 |
nsubj |
excision,abolished |
R2861 |
T13336 |
T13335 |
prep |
of,excision |
R2862 |
T13405 |
T13406 |
det |
the,time |
R2863 |
T13406 |
T13404 |
pobj |
time,in |
R2864 |
T13337 |
T13338 |
det |
the,acid |
R2865 |
T13407 |
T13406 |
prep |
of,time |
R2866 |
T13408 |
T13407 |
pobj |
lethality,of |
R2867 |
T13409 |
T13406 |
prep |
of,time |
R2868 |
T13338 |
T13336 |
pobj |
acid,of |
R2869 |
T13410 |
T13411 |
det |
these,lines |
R2870 |
T13339 |
T13340 |
npadvmod |
C,terminal |
R2871 |
T13340 |
T13338 |
amod |
terminal,acid |
R2872 |
T13411 |
T13409 |
pobj |
lines,of |
R2873 |
T13341 |
T13340 |
punct |
-,terminal |
R2874 |
T13412 |
T13411 |
nummod |
two,lines |
R2875 |
T13413 |
T13411 |
amod |
targeted,lines |
R2876 |
T13414 |
T13411 |
compound |
Capn4,lines |
R2877 |
T13415 |
T13401 |
aux |
can,explained |
R2878 |
T13416 |
T13401 |
auxpass |
be,explained |
R2879 |
T13417 |
T13401 |
agent |
by,explained |
R2880 |
T13418 |
T13419 |
amod |
different,extents |
R2881 |
T13342 |
T13338 |
nummod |
25,acid |
R2882 |
T13419 |
T13417 |
pobj |
extents,by |
R2883 |
T13420 |
T13419 |
prep |
of,extents |
R2884 |
T13421 |
T13420 |
pobj |
disruption,of |
R2885 |
T13343 |
T13338 |
compound |
amino,acid |
R2886 |
T13422 |
T13397 |
punct |
.,seems |
R2887 |
T13344 |
T13338 |
nmod |
residues,acid |
R2888 |
T13424 |
T13425 |
det |
The,allele |
R2889 |
T13425 |
T13427 |
nsubj |
allele,represents |
R2890 |
T13345 |
T13338 |
prep |
of,acid |
R2891 |
T13426 |
T13425 |
compound |
Zimmerman,allele |
R2892 |
T13346 |
T13347 |
det |
the,subunit |
R2893 |
T13428 |
T13427 |
advmod |
probably,represents |
R2894 |
T13429 |
T13430 |
det |
a,genotype |
R2895 |
T13430 |
T13427 |
dobj |
genotype,represents |
R2896 |
T13347 |
T13345 |
pobj |
subunit,of |
R2897 |
T13431 |
T13430 |
amod |
true,genotype |
R2898 |
T13432 |
T13430 |
amod |
null,genotype |
R2899 |
T13433 |
T13434 |
mark |
while,is |
R2900 |
T13348 |
T13347 |
amod |
small,subunit |
R2901 |
T13434 |
T13427 |
advcl |
is,represents |
R2902 |
T13435 |
T13436 |
det |
the,allele |
R2903 |
T13436 |
T13434 |
nsubj |
allele,is |
R2904 |
T13349 |
T13350 |
compound |
calpain,activity |
R2905 |
T13437 |
T13436 |
compound |
Arthur,allele |
R2906 |
T13438 |
T13434 |
advmod |
likely,is |
R2907 |
T13439 |
T13440 |
det |
a,mutation |
R2908 |
T13350 |
T13334 |
dobj |
activity,abolished |
R2909 |
T13440 |
T13434 |
attr |
mutation,is |
R2910 |
T13441 |
T13440 |
amod |
hypomorphic,mutation |
R2911 |
T13442 |
T13427 |
punct |
.,represents |
R2912 |
T13351 |
T13352 |
advmod |
when,co-expressed |
R2913 |
T13444 |
T13445 |
amod |
Alternate,strategies |
R2914 |
T13352 |
T13334 |
advcl |
co-expressed,abolished |
R2915 |
T13445 |
T13447 |
nsubjpass |
strategies,shown |
R2916 |
T13446 |
T13445 |
compound |
targeting,strategies |
R2917 |
T13353 |
T13352 |
prep |
with,co-expressed |
R2918 |
T13448 |
T13447 |
aux |
have,shown |
R2919 |
T13449 |
T13447 |
auxpass |
been,shown |
R2920 |
T13450 |
T13451 |
aux |
to,yield |
R2921 |
T13354 |
T13355 |
det |
the,subunit |
R2922 |
T13451 |
T13447 |
advcl |
yield,shown |
R2923 |
T13452 |
T13453 |
amod |
different,phenotypes |
R2924 |
T13453 |
T13451 |
dobj |
phenotypes,yield |
R2925 |
T13355 |
T13353 |
pobj |
subunit,with |
R2926 |
T13454 |
T13451 |
prep |
in,yield |
R2927 |
T13455 |
T13456 |
compound |
gene,disruption |
R2928 |
T13456 |
T13457 |
compound |
disruption,studies |
R2929 |
T13457 |
T13454 |
pobj |
studies,in |
R2930 |
T13458 |
T13447 |
punct |
.,shown |
R2931 |
T13460 |
T13461 |
prep |
For,used |
R2932 |
T13356 |
T13357 |
nmod |
m,calpain |
R2933 |
T13462 |
T13460 |
pobj |
example,For |
R2934 |
T13463 |
T13461 |
punct |
", ",used |
R2935 |
T13464 |
T13465 |
nummod |
three,strategies |
R2936 |
T13465 |
T13461 |
nsubjpass |
strategies,used |
R2937 |
T13466 |
T13465 |
amod |
different,strategies |
R2938 |
T13357 |
T13355 |
nmod |
calpain,subunit |
R2939 |
T13467 |
T13465 |
compound |
targeting,strategies |
R2940 |
T13468 |
T13461 |
auxpass |
were,used |
R2941 |
T13469 |
T13461 |
advmod |
independently,used |
R2942 |
T13358 |
T13357 |
punct |
-,calpain |
R2943 |
T13470 |
T13471 |
aux |
to,disrupt |
R2944 |
T13471 |
T13461 |
advcl |
disrupt,used |
R2945 |
T13359 |
T13355 |
amod |
large,subunit |
R2946 |
T13472 |
T13473 |
det |
the,gene |
R2947 |
T13473 |
T13471 |
dobj |
gene,disrupt |
R2948 |
T13474 |
T13475 |
amod |
mixed,leukemia |
R2949 |
T13475 |
T13473 |
nmod |
leukemia,gene |
R2950 |
T13360 |
T13352 |
prep |
in,co-expressed |
R2951 |
T13476 |
T13475 |
nmod |
lineage,leukemia |
R2952 |
T13477 |
T13475 |
punct |
(,leukemia |
R2953 |
T13361 |
T13362 |
compound |
E.,coli |
R2954 |
T13478 |
T13475 |
appos |
Mll,leukemia |
R2955 |
T13479 |
T13473 |
punct |
),gene |
R2956 |
T13480 |
T13461 |
punct |
.,used |
R2957 |
T13362 |
T13360 |
pobj |
coli,in |
R2958 |
T13482 |
T13483 |
prep |
In,perished |
R2959 |
T13484 |
T13485 |
det |
all,studies |
R2960 |
T13363 |
T13364 |
punct |
[,34 |
R2961 |
T13485 |
T13482 |
pobj |
studies,In |
R2962 |
T13486 |
T13485 |
nummod |
three,studies |
R2963 |
T13487 |
T13483 |
punct |
", ",perished |
R2964 |
T13364 |
T13334 |
parataxis |
34,abolished |
R2965 |
T13488 |
T13489 |
amod |
homozygous,null |
R2966 |
T13489 |
T13490 |
amod |
null,embryos |
R2967 |
T13490 |
T13483 |
nsubj |
embryos,perished |
R2968 |
T13365 |
T13364 |
punct |
],34 |
R2969 |
T13491 |
T13483 |
prep |
during,perished |
R2970 |
T13492 |
T13491 |
pobj |
embryogenesis,during |
R2971 |
T13366 |
T13324 |
punct |
.,based |
R2972 |
T13493 |
T13491 |
punct |
", ",during |
R2973 |
T13494 |
T13491 |
cc |
but,during |
R2974 |
T13495 |
T13491 |
conj |
at,during |
R2975 |
T13496 |
T13497 |
amod |
different,stages |
R2976 |
T13368 |
T13369 |
prep |
In,involved |
R2977 |
T13497 |
T13495 |
pobj |
stages,at |
R2978 |
T13498 |
T13499 |
punct |
(,E14.5 |
R2979 |
T13499 |
T13497 |
parataxis |
E14.5,stages |
R2980 |
T13500 |
T13499 |
nmod |
E0.5,E14.5 |
R2981 |
T13501 |
T13499 |
punct |
", ",E14.5 |
R2982 |
T13370 |
T13368 |
pobj |
contrast,In |
R2983 |
T13502 |
T13499 |
nmod |
E10.5,E14.5 |
R2984 |
T13503 |
T13499 |
punct |
", ",E14.5 |
R2985 |
T13504 |
T13499 |
punct |
),E14.5 |
R2986 |
T13505 |
T13506 |
punct |
[,35 |
R2987 |
T13506 |
T13483 |
parataxis |
35,perished |
R2988 |
T13371 |
T13369 |
punct |
", ",involved |
R2989 |
T13507 |
T13506 |
punct |
],35 |
R2990 |
T13508 |
T13483 |
punct |
.,perished |
R2991 |
T13372 |
T13373 |
det |
the,strategy |
R2992 |
T13510 |
T13511 |
det |
The,variation |
R2993 |
T13373 |
T13369 |
nsubj |
strategy,involved |
R2994 |
T13374 |
T13373 |
nmod |
Capn4,strategy |
R2995 |
T13375 |
T13373 |
amod |
targeting,strategy |
R2996 |
T13511 |
T13512 |
nsubjpass |
variation,attributed |
R2997 |
T13376 |
T13373 |
acl |
employed,strategy |
R2998 |
T13513 |
T13511 |
prep |
in,variation |
R2999 |
T13377 |
T13376 |
agent |
by,employed |
R3000 |
T13514 |
T13513 |
pobj |
phenotype,in |
R3001 |
T13515 |
T13512 |
auxpass |
was,attributed |
R3002 |
T13516 |
T13512 |
prep |
to,attributed |
R3003 |
T13517 |
T13518 |
det |
the,differences |
R3004 |
T13378 |
T13377 |
pobj |
Zimmerman,by |
R3005 |
T13518 |
T13516 |
pobj |
differences,to |
R3006 |
T13519 |
T13518 |
prep |
in,differences |
R3007 |
T13520 |
T13519 |
pobj |
degree,in |
R3008 |
T13379 |
T13378 |
cc |
and,Zimmerman |
R3009 |
T13521 |
T13520 |
prep |
of,degree |
R3010 |
T13522 |
T13521 |
pobj |
function,of |
R3011 |
T13523 |
T13520 |
prep |
of,degree |
R3012 |
T13380 |
T13378 |
conj |
colleagues,Zimmerman |
R3013 |
T13524 |
T13525 |
det |
the,proteins |
R3014 |
T13525 |
T13523 |
pobj |
proteins,of |
R3015 |
T13526 |
T13525 |
amod |
truncated,proteins |
R3016 |
T13381 |
T13382 |
det |
a,deletion |
R3017 |
T13527 |
T13525 |
acl |
produced,proteins |
R3018 |
T13528 |
T13527 |
prep |
from,produced |
R3019 |
T13529 |
T13530 |
det |
the,alleles |
R3020 |
T13382 |
T13369 |
dobj |
deletion,involved |
R3021 |
T13530 |
T13528 |
pobj |
alleles,from |
R3022 |
T13531 |
T13530 |
compound |
mutant,alleles |
R3023 |
T13532 |
T13512 |
punct |
.,attributed |
R3024 |
T13383 |
T13384 |
advmod |
much,more |
R3025 |
T13534 |
T13535 |
det |
A,effect |
R3026 |
T13535 |
T13537 |
nsubj |
effect,be |
R3027 |
T13384 |
T13385 |
advmod |
more,extensive |
R3028 |
T13536 |
T13535 |
amod |
similar,effect |
R3029 |
T13538 |
T13537 |
aux |
might,be |
R3030 |
T13385 |
T13382 |
amod |
extensive,deletion |
R3031 |
T13539 |
T13537 |
prep |
at,be |
R3032 |
T13540 |
T13539 |
pobj |
work,at |
R3033 |
T13541 |
T13537 |
prep |
in,be |
R3034 |
T13542 |
T13543 |
det |
the,lines |
R3035 |
T13543 |
T13541 |
pobj |
lines,in |
R3036 |
T13544 |
T13543 |
nummod |
two,lines |
R3037 |
T13386 |
T13382 |
prep |
of,deletion |
R3038 |
T13545 |
T13546 |
npadvmod |
Capn4,transgenic |
R3039 |
T13546 |
T13543 |
amod |
transgenic,lines |
R3040 |
T13547 |
T13537 |
punct |
.,be |
R3041 |
T13387 |
T13388 |
nmod |
exons,4 |
R3042 |
T13549 |
T13550 |
nsubjpass |
Efforts,made |
R3043 |
T13388 |
T13386 |
pobj |
4,of |
R3044 |
T13551 |
T13550 |
auxpass |
were,made |
R3045 |
T13552 |
T13553 |
aux |
to,detect |
R3046 |
T13553 |
T13550 |
advcl |
detect,made |
R3047 |
T13389 |
T13388 |
prep |
through,4 |
R3048 |
T13554 |
T13553 |
dobj |
transcripts,detect |
R3049 |
T13555 |
T13554 |
cc |
or,transcripts |
R3050 |
T13390 |
T13389 |
pobj |
8,through |
R3051 |
T13556 |
T13557 |
compound |
calpain,activities |
R3052 |
T13557 |
T13554 |
conj |
activities,transcripts |
R3053 |
T13391 |
T13392 |
punct |
[,32 |
R3054 |
T13558 |
T13554 |
acl |
derived,transcripts |
R3055 |
T13559 |
T13558 |
prep |
from,derived |
R3056 |
T13560 |
T13561 |
det |
the,allele |
R3057 |
T13561 |
T13559 |
pobj |
allele,from |
R3058 |
T13392 |
T13369 |
parataxis |
32,involved |
R3059 |
T13562 |
T13563 |
compound |
Arthur,Capn4 |
R3060 |
T13563 |
T13561 |
compound |
Capn4,allele |
R3061 |
T13564 |
T13550 |
punct |
.,made |
R3062 |
T13393 |
T13392 |
punct |
],32 |
R3063 |
T13566 |
T13567 |
det |
This,allele |
R3064 |
T13567 |
T13568 |
nsubj |
allele,gave |
R3065 |
T13394 |
T13369 |
punct |
.,involved |
R3066 |
T13569 |
T13568 |
dobj |
rise,gave |
R3067 |
T13396 |
T13397 |
nsubj |
It,seems |
R3068 |
T13570 |
T13568 |
prep |
to,gave |
R3069 |
T13571 |
T13572 |
amod |
multiple,species |
R3070 |
T13572 |
T13570 |
pobj |
species,to |
R3071 |
T13573 |
T13572 |
compound |
mRNA,species |
R3072 |
T13398 |
T13397 |
advmod |
now,seems |
R3073 |
T13574 |
T13572 |
punct |
", ",species |
R3074 |
T13575 |
T13572 |
amod |
detectable,species |
R3075 |
T13576 |
T13575 |
prep |
by,detectable |
R3076 |
T13577 |
T13578 |
compound |
RT,PCR |
R3077 |
T13399 |
T13397 |
oprd |
probable,seems |
R3078 |
T13578 |
T13576 |
pobj |
PCR,by |
R3079 |
T13579 |
T13578 |
punct |
-,PCR |
R3080 |
T13580 |
T13568 |
punct |
", ",gave |
R3081 |
T13400 |
T13401 |
mark |
that,explained |
R3082 |
T13581 |
T13568 |
advcl |
reading,gave |
R3083 |
T13582 |
T13581 |
advmod |
through,reading |
R3084 |
T13583 |
T13581 |
prep |
from,reading |
R3085 |
T13584 |
T13585 |
det |
the,half |
R3086 |
T13585 |
T13583 |
pobj |
half,from |
R3087 |
T13401 |
T13397 |
ccomp |
explained,seems |
R3088 |
T13586 |
T13585 |
amod |
first,half |
R3089 |
T13587 |
T13585 |
prep |
of,half |
R3090 |
T13588 |
T13587 |
pobj |
exon,of |
R3091 |
T13402 |
T13403 |
det |
the,difference |
R3092 |
T13403 |
T13401 |
nsubjpass |
difference,explained |
R3093 |
T13589 |
T13588 |
nummod |
9,exon |
R3094 |
T13590 |
T13583 |
prep |
to,from |
R3095 |
T13404 |
T13403 |
prep |
in,difference |
R3096 |
T13591 |
T13592 |
advmod |
at,two |
R3097 |
T13592 |
T13594 |
nummod |
two,sites |
R3098 |
T13593 |
T13592 |
advmod |
least,two |
R3099 |
T13617 |
T13615 |
amod |
small,subunits |
R3100 |
T13594 |
T13590 |
pobj |
sites,to |
R3101 |
T13595 |
T13594 |
amod |
different,sites |
R3102 |
T13596 |
T13594 |
amod |
cryptic,sites |
R3103 |
T13597 |
T13594 |
compound |
splice,sites |
R3104 |
T13618 |
T13610 |
prep |
with,give |
R3105 |
T13598 |
T13594 |
prep |
in,sites |
R3106 |
T13599 |
T13600 |
det |
the,sequence |
R3107 |
T13619 |
T13620 |
quantmod |
10,30 |
R3108 |
T13600 |
T13598 |
pobj |
sequence,in |
R3109 |
T13601 |
T13600 |
compound |
PGK,sequence |
R3110 |
T13602 |
T13600 |
compound |
promoter,sequence |
R3111 |
T13620 |
T13622 |
nummod |
30,acids |
R3112 |
T13603 |
T13604 |
punct |
[,31 |
R3113 |
T13604 |
T13581 |
parataxis |
31,reading |
R3114 |
T13605 |
T13604 |
punct |
],31 |
R3115 |
T13621 |
T13620 |
punct |
–,30 |
R3116 |
T13606 |
T13568 |
punct |
.,gave |
R3117 |
T13608 |
T13609 |
det |
These,transcripts |
R3118 |
T13622 |
T13618 |
pobj |
acids,with |
R3119 |
T13609 |
T13610 |
nsubj |
transcripts,give |
R3120 |
T13611 |
T13610 |
aux |
could,give |
R3121 |
T13623 |
T13622 |
amod |
inappropriate,acids |
R3122 |
T13612 |
T13610 |
dobj |
rise,give |
R3123 |
T13613 |
T13610 |
prep |
to,give |
R3124 |
T13614 |
T13615 |
amod |
defective,subunits |
R3125 |
T13624 |
T13625 |
npadvmod |
C,terminal |
R3126 |
T13615 |
T13613 |
pobj |
subunits,to |
R3127 |
T13625 |
T13622 |
amod |
terminal,acids |
R3128 |
T13626 |
T13625 |
punct |
-,terminal |
R3129 |
T13616 |
T13615 |
nmod |
calpain,subunits |
R3130 |
T13627 |
T13622 |
punct |
", ",acids |
R3131 |
T13628 |
T13629 |
dep |
which,be |
R3132 |
T13629 |
T13622 |
relcl |
be,acids |
R3133 |
T13723 |
T13721 |
pobj |
hands,in |
R3134 |
T13630 |
T13629 |
aux |
might,be |
R3135 |
T13724 |
T13725 |
advmod |
when,expressed |
R3136 |
T13725 |
T13719 |
advcl |
expressed,observed |
R3137 |
T13726 |
T13727 |
nmod |
calpain,subunits |
R3138 |
T13727 |
T13725 |
nsubjpass |
subunits,expressed |
R3139 |
T13728 |
T13727 |
amod |
large,subunits |
R3140 |
T13729 |
T13725 |
auxpass |
were,expressed |
R3141 |
T13631 |
T13629 |
acomp |
sufficient,be |
R3142 |
T13730 |
T13725 |
advmod |
alone,expressed |
R3143 |
T13731 |
T13732 |
preconj |
either,in |
R3144 |
T13732 |
T13725 |
prep |
in,expressed |
R3145 |
T13632 |
T13633 |
aux |
to,support |
R3146 |
T13733 |
T13734 |
compound |
E.,coli |
R3147 |
T13734 |
T13732 |
pobj |
coli,in |
R3148 |
T13735 |
T13732 |
cc |
or,in |
R3149 |
T13633 |
T13631 |
xcomp |
support,sufficient |
R3150 |
T13736 |
T13732 |
conj |
in,in |
R3151 |
T13737 |
T13738 |
amod |
mammalian,cells |
R3152 |
T13634 |
T13635 |
det |
a,level |
R3153 |
T13738 |
T13736 |
pobj |
cells,in |
R3154 |
T13739 |
T13740 |
punct |
[,36 |
R3155 |
T13740 |
T13725 |
parataxis |
36,expressed |
R3156 |
T13635 |
T13633 |
dobj |
level,support |
R3157 |
T13741 |
T13740 |
punct |
],36 |
R3158 |
T13742 |
T13655 |
punct |
.,suggested |
R3159 |
T13636 |
T13635 |
amod |
low,level |
R3160 |
T13744 |
T13745 |
det |
The,timing |
R3161 |
T13745 |
T13747 |
nsubj |
timing,be |
R3162 |
T13637 |
T13635 |
prep |
of,level |
R3163 |
T13746 |
T13745 |
amod |
different,timing |
R3164 |
T13638 |
T13639 |
compound |
calpain,activity |
R3165 |
T13748 |
T13745 |
prep |
of,timing |
R3166 |
T13749 |
T13748 |
pobj |
lethality,of |
R3167 |
T13750 |
T13745 |
prep |
in,timing |
R3168 |
T13639 |
T13637 |
pobj |
activity,of |
R3169 |
T13751 |
T13752 |
det |
the,knockouts |
R3170 |
T13752 |
T13750 |
pobj |
knockouts,in |
R3171 |
T13753 |
T13752 |
compound |
Capn4,knockouts |
R3172 |
T13640 |
T13610 |
punct |
.,give |
R3173 |
T13754 |
T13747 |
aux |
might,be |
R3174 |
T13755 |
T13747 |
advmod |
also,be |
R3175 |
T13756 |
T13757 |
det |
a,consequence |
R3176 |
T13642 |
T13643 |
advmod |
However,give |
R3177 |
T13643 |
T13655 |
ccomp |
give,suggested |
R3178 |
T13757 |
T13747 |
attr |
consequence,be |
R3179 |
T13758 |
T13757 |
prep |
of,consequence |
R3180 |
T13644 |
T13643 |
punct |
", ",give |
R3181 |
T13759 |
T13760 |
det |
the,backgrounds |
R3182 |
T13760 |
T13758 |
pobj |
backgrounds,of |
R3183 |
T13761 |
T13760 |
amod |
different,backgrounds |
R3184 |
T13645 |
T13643 |
nsubj |
expression,give |
R3185 |
T13762 |
T13760 |
amod |
genetic,backgrounds |
R3186 |
T13763 |
T13760 |
prep |
of,backgrounds |
R3187 |
T13764 |
T13765 |
det |
the,lines |
R3188 |
T13765 |
T13763 |
pobj |
lines,of |
R3189 |
T13766 |
T13765 |
nummod |
two,lines |
R3190 |
T13646 |
T13645 |
prep |
of,expression |
R3191 |
T13767 |
T13765 |
amod |
transgenic,lines |
R3192 |
T13768 |
T13757 |
punct |
", ",consequence |
R3193 |
T13769 |
T13770 |
dep |
which,observed |
R3194 |
T13647 |
T13646 |
pobj |
calpains,of |
R3195 |
T13770 |
T13757 |
relcl |
observed,consequence |
R3196 |
T13771 |
T13770 |
aux |
has,observed |
R3197 |
T13772 |
T13770 |
auxpass |
been,observed |
R3198 |
T13648 |
T13647 |
prep |
with,calpains |
R3199 |
T13773 |
T13774 |
aux |
to,influence |
R3200 |
T13774 |
T13770 |
advcl |
influence,observed |
R3201 |
T13649 |
T13650 |
det |
these,subunits |
R3202 |
T13775 |
T13776 |
det |
the,phenotype |
R3203 |
T13776 |
T13774 |
dobj |
phenotype,influence |
R3204 |
T13777 |
T13776 |
prep |
of,phenotype |
R3205 |
T13650 |
T13648 |
pobj |
subunits,with |
R3206 |
T13778 |
T13779 |
amod |
transgenic,mice |
R3207 |
T13779 |
T13777 |
pobj |
mice,of |
R3208 |
T13651 |
T13650 |
amod |
modified,subunits |
R3209 |
T13780 |
T13774 |
prep |
on,influence |
R3210 |
T13781 |
T13782 |
det |
a,number |
R3211 |
T13782 |
T13780 |
pobj |
number,on |
R3212 |
T13652 |
T13650 |
amod |
small,subunits |
R3213 |
T13783 |
T13782 |
prep |
of,number |
R3214 |
T13784 |
T13783 |
pobj |
occasions,of |
R3215 |
T13785 |
T13786 |
punct |
[,37 |
R3216 |
T13653 |
T13643 |
aux |
did,give |
R3217 |
T13786 |
T13774 |
parataxis |
37,influence |
R3218 |
T13787 |
T13786 |
punct |
],37 |
R3219 |
T13654 |
T13643 |
neg |
not,give |
R3220 |
T13788 |
T13747 |
punct |
.,be |
R3221 |
T13790 |
T13791 |
nsubj |
One,been |
R3222 |
T13656 |
T13643 |
dobj |
rise,give |
R3223 |
T13792 |
T13790 |
prep |
of,One |
R3224 |
T13793 |
T13794 |
det |
the,questions |
R3225 |
T13794 |
T13792 |
pobj |
questions,of |
R3226 |
T13657 |
T13643 |
prep |
to,give |
R3227 |
T13795 |
T13794 |
amod |
enduring,questions |
R3228 |
T13796 |
T13794 |
prep |
in,questions |
R3229 |
T13797 |
T13798 |
compound |
calpain,research |
R3230 |
T13658 |
T13659 |
det |
any,activity |
R3231 |
T13798 |
T13796 |
pobj |
research,in |
R3232 |
T13799 |
T13791 |
aux |
has,been |
R3233 |
T13800 |
T13801 |
mark |
whether,possess |
R3234 |
T13659 |
T13657 |
pobj |
activity,to |
R3235 |
T13801 |
T13791 |
ccomp |
possess,been |
R3236 |
T13802 |
T13803 |
det |
the,isoforms |
R3237 |
T13803 |
T13801 |
nsubj |
isoforms,possess |
R3238 |
T13660 |
T13659 |
amod |
detectable,activity |
R3239 |
T13804 |
T13803 |
nummod |
two,isoforms |
R3240 |
T13805 |
T13803 |
amod |
ubiquitous,isoforms |
R3241 |
T13806 |
T13803 |
punct |
", ",isoforms |
R3242 |
T13807 |
T13808 |
nmod |
μ,calpain |
R3243 |
T13808 |
T13803 |
appos |
calpain,isoforms |
R3244 |
T13661 |
T13659 |
compound |
calpain,activity |
R3245 |
T13809 |
T13807 |
punct |
-,μ |
R3246 |
T13810 |
T13807 |
cc |
and,μ |
R3247 |
T13811 |
T13807 |
conj |
m,μ |
R3248 |
T13812 |
T13808 |
punct |
-,calpain |
R3249 |
T13662 |
T13663 |
advmod |
when,expressed |
R3250 |
T13813 |
T13801 |
punct |
", ",possess |
R3251 |
T13814 |
T13815 |
amod |
distinct,roles |
R3252 |
T13663 |
T13643 |
advcl |
expressed,give |
R3253 |
T13815 |
T13801 |
dobj |
roles,possess |
R3254 |
T13816 |
T13817 |
advmod |
in,vivo |
R3255 |
T13817 |
T13815 |
amod |
vivo,roles |
R3256 |
T13664 |
T13663 |
prep |
in,expressed |
R3257 |
T13818 |
T13791 |
punct |
.,been |
R3258 |
T13820 |
T13821 |
det |
The,enzymes |
R3259 |
T13665 |
T13666 |
compound |
E.,coli |
R3260 |
T13821 |
T13823 |
nsubj |
enzymes,share |
R3261 |
T13822 |
T13821 |
nummod |
two,enzymes |
R3262 |
T13666 |
T13664 |
pobj |
coli,in |
R3263 |
T13824 |
T13825 |
nummod |
62,% |
R3264 |
T13825 |
T13826 |
compound |
%,identity |
R3265 |
T13667 |
T13643 |
punct |
", ",give |
R3266 |
T13826 |
T13823 |
dobj |
identity,share |
R3267 |
T13827 |
T13826 |
compound |
sequence,identity |
R3268 |
T13828 |
T13823 |
cc |
and,share |
R3269 |
T13668 |
T13669 |
mark |
although,has |
R3270 |
T13669 |
T13643 |
advcl |
has,give |
R3271 |
T13670 |
T13671 |
poss |
their,functionality |
R3272 |
T13829 |
T13823 |
conj |
are,share |
R3273 |
T13830 |
T13831 |
advmod |
very,similar |
R3274 |
T13671 |
T13669 |
nsubj |
functionality,has |
R3275 |
T13831 |
T13829 |
acomp |
similar,are |
R3276 |
T13832 |
T13829 |
prep |
in,are |
R3277 |
T13833 |
T13834 |
poss |
their,structure |
R3278 |
T13672 |
T13671 |
prep |
in,functionality |
R3279 |
T13834 |
T13832 |
pobj |
structure,in |
R3280 |
T13835 |
T13834 |
cc |
and,structure |
R3281 |
T13673 |
T13674 |
amod |
mammalian,cells |
R3282 |
T13836 |
T13834 |
conj |
biochemistry,structure |
R3283 |
T13837 |
T13823 |
punct |
.,share |
R3284 |
T13674 |
T13672 |
pobj |
cells,in |
R3285 |
T13839 |
T13840 |
advmod |
Notably,cleave |
R3286 |
T13675 |
T13669 |
advmod |
yet,has |
R3287 |
T13841 |
T13840 |
punct |
", ",cleave |
R3288 |
T13842 |
T13840 |
nsubj |
they,cleave |
R3289 |
T13843 |
T13840 |
advmod |
essentially,cleave |
R3290 |
T13676 |
T13677 |
aux |
to,determined |
R3291 |
T13677 |
T13669 |
xcomp |
determined,has |
R3292 |
T13844 |
T13845 |
det |
the,set |
R3293 |
T13845 |
T13840 |
dobj |
set,cleave |
R3294 |
T13678 |
T13677 |
auxpass |
be,determined |
R3295 |
T13846 |
T13845 |
amod |
same,set |
R3296 |
T13847 |
T13845 |
prep |
of,set |
R3297 |
T13848 |
T13847 |
pobj |
substrates,of |
R3298 |
T13679 |
T13680 |
punct |
(,J.S. |
R3299 |
T13849 |
T13850 |
advmod |
in,vitro |
R3300 |
T13850 |
T13840 |
advmod |
vitro,cleave |
R3301 |
T13680 |
T13669 |
meta |
J.S.,has |
R3302 |
T13851 |
T13840 |
punct |
", ",cleave |
R3303 |
T13852 |
T13840 |
advcl |
suggesting,cleave |
R3304 |
T13681 |
T13680 |
nmod |
Elce,J.S. |
R3305 |
T13853 |
T13854 |
mark |
that,have |
R3306 |
T13854 |
T13852 |
ccomp |
have,suggesting |
R3307 |
T13855 |
T13854 |
nsubj |
they,have |
R3308 |
T13682 |
T13680 |
punct |
", ",J.S. |
R3309 |
T13856 |
T13857 |
det |
the,potential |
R3310 |
T13857 |
T13854 |
dobj |
potential,have |
R3311 |
T13683 |
T13680 |
amod |
unpublished,J.S. |
R3312 |
T13858 |
T13859 |
aux |
to,carry |
R3313 |
T13859 |
T13857 |
acl |
carry,potential |
R3314 |
T13860 |
T13859 |
prt |
out,carry |
R3315 |
T13684 |
T13680 |
nmod |
work,J.S. |
R3316 |
T13861 |
T13862 |
det |
the,functions |
R3317 |
T13862 |
T13859 |
dobj |
functions,carry |
R3318 |
T13863 |
T13862 |
amod |
same,functions |
R3319 |
T13685 |
T13680 |
punct |
),J.S. |
R3320 |
T13864 |
T13865 |
advmod |
in,vivo |
R3321 |
T13865 |
T13859 |
advmod |
vivo,carry |
R3322 |
T13686 |
T13655 |
nsubjpass |
It,suggested |
R3323 |
T13866 |
T13840 |
punct |
.,cleave |
R3324 |
T13687 |
T13655 |
aux |
has,suggested |
R3325 |
T13868 |
T13869 |
prep |
On,is |
R3326 |
T13688 |
T13655 |
advmod |
also,suggested |
R3327 |
T13870 |
T13871 |
det |
the,hand |
R3328 |
T13871 |
T13868 |
pobj |
hand,On |
R3329 |
T13872 |
T13871 |
amod |
other,hand |
R3330 |
T13689 |
T13655 |
auxpass |
been,suggested |
R3331 |
T13873 |
T13869 |
punct |
", ",is |
R3332 |
T13874 |
T13875 |
mark |
since,require |
R3333 |
T13875 |
T13869 |
advcl |
require,is |
R3334 |
T13690 |
T13691 |
mark |
that,provide |
R3335 |
T13876 |
T13875 |
nsubj |
they,require |
R3336 |
T13877 |
T13878 |
amod |
different,amounts |
R3337 |
T13878 |
T13875 |
dobj |
amounts,require |
R3338 |
T13691 |
T13655 |
ccomp |
provide,suggested |
R3339 |
T13879 |
T13878 |
prep |
of,amounts |
R3340 |
T13880 |
T13879 |
pobj |
Ca2,of |
R3341 |
T13881 |
T13880 |
punct |
+,Ca2 |
R3342 |
T13692 |
T13693 |
nmod |
calpain,subunits |
R3343 |
T13882 |
T13875 |
prep |
for,require |
R3344 |
T13883 |
T13884 |
nmod |
in,vitro |
R3345 |
T13884 |
T13885 |
nmod |
vitro,activation |
R3346 |
T13885 |
T13882 |
pobj |
activation,for |
R3347 |
T13886 |
T13869 |
punct |
", ",is |
R3348 |
T13693 |
T13691 |
nsubj |
subunits,provide |
R3349 |
T13887 |
T13869 |
nsubj |
it,is |
R3350 |
T13888 |
T13869 |
acomp |
possible,is |
R3351 |
T13889 |
T13890 |
mark |
that,regulated |
R3352 |
T13694 |
T13693 |
amod |
large,subunits |
R3353 |
T13890 |
T13869 |
ccomp |
regulated,is |
R3354 |
T13891 |
T13892 |
det |
the,isoforms |
R3355 |
T13695 |
T13693 |
advmod |
alone,subunits |
R3356 |
T13892 |
T13890 |
nsubjpass |
isoforms,regulated |
R3357 |
T13893 |
T13892 |
nummod |
two,isoforms |
R3358 |
T13696 |
T13691 |
aux |
might,provide |
R3359 |
T13894 |
T13890 |
auxpass |
are,regulated |
R3360 |
T13895 |
T13890 |
advmod |
differentially,regulated |
R3361 |
T13896 |
T13890 |
prep |
inside,regulated |
R3362 |
T13697 |
T13698 |
det |
some,activity |
R3363 |
T13897 |
T13896 |
pobj |
cells,inside |
R3364 |
T13898 |
T13869 |
punct |
.,is |
R3365 |
T13698 |
T13691 |
dobj |
activity,provide |
R3366 |
T13900 |
T13901 |
nsubj |
It,is |
R3367 |
T13699 |
T13691 |
prep |
in,provide |
R3368 |
T13902 |
T13901 |
advmod |
now,is |
R3369 |
T13903 |
T13901 |
acomp |
clear,is |
R3370 |
T13904 |
T13901 |
punct |
", ",is |
R3371 |
T13700 |
T13701 |
amod |
eukaryotic,cells |
R3372 |
T13905 |
T13901 |
prep |
from,is |
R3373 |
T13906 |
T13907 |
det |
the,work |
R3374 |
T13907 |
T13905 |
pobj |
work,from |
R3375 |
T13701 |
T13699 |
pobj |
cells,in |
R3376 |
T13908 |
T13909 |
compound |
gene,targeting |
R3377 |
T13909 |
T13907 |
compound |
targeting,work |
R3378 |
T13910 |
T13907 |
acl |
done,work |
R3379 |
T13702 |
T13691 |
punct |
", ",provide |
R3380 |
T13911 |
T13910 |
prep |
in,done |
R3381 |
T13912 |
T13911 |
pobj |
mice,in |
R3382 |
T13703 |
T13704 |
mark |
although,appears |
R3383 |
T13913 |
T13901 |
punct |
", ",is |
R3384 |
T13914 |
T13915 |
mark |
that,have |
R3385 |
T13915 |
T13901 |
ccomp |
have,is |
R3386 |
T13704 |
T13691 |
advcl |
appears,provide |
R3387 |
T13916 |
T13917 |
nmod |
μ,calpain |
R3388 |
T13917 |
T13915 |
nsubj |
calpain,have |
R3389 |
T13918 |
T13916 |
punct |
-,μ |
R3390 |
T13705 |
T13706 |
det |
the,knockout |
R3391 |
T13919 |
T13916 |
cc |
and,μ |
R3392 |
T13920 |
T13916 |
conj |
m,μ |
R3393 |
T13921 |
T13917 |
punct |
-,calpain |
R3394 |
T13706 |
T13704 |
nsubj |
knockout,appears |
R3395 |
T13922 |
T13923 |
det |
some,roles |
R3396 |
T13923 |
T13915 |
dobj |
roles,have |
R3397 |
T13924 |
T13923 |
amod |
distinct,roles |
R3398 |
T13925 |
T13923 |
amod |
physiological,roles |
R3399 |
T13926 |
T13915 |
punct |
", ",have |
R3400 |
T13707 |
T13706 |
nmod |
Zimmerman,knockout |
R3401 |
T13927 |
T13928 |
advmod |
at,least |
R3402 |
T13928 |
T13929 |
advmod |
least,during |
R3403 |
T13708 |
T13709 |
advmod |
et,al. |
R3404 |
T13929 |
T13915 |
prep |
during,have |
R3405 |
T13709 |
T13707 |
advmod |
al.,Zimmerman |
R3406 |
T13930 |
T13929 |
pobj |
embryogenesis,during |
R3407 |
T13931 |
T13901 |
punct |
.,is |
R3408 |
T13710 |
T13711 |
aux |
to,exclude |
R3409 |
T13933 |
T13934 |
mark |
As,noted |
R3410 |
T13711 |
T13704 |
xcomp |
exclude,appears |
R3411 |
T13934 |
T13935 |
advcl |
noted,affect |
R3412 |
T13712 |
T13713 |
det |
that,possibility |
R3413 |
T13713 |
T13711 |
dobj |
possibility,exclude |
R3414 |
T13936 |
T13935 |
punct |
", ",affect |
R3415 |
T13714 |
T13691 |
punct |
", ",provide |
R3416 |
T13937 |
T13938 |
mark |
whereas,die |
R3417 |
T13938 |
T13935 |
advcl |
die,affect |
R3418 |
T13715 |
T13691 |
cc |
and,provide |
R3419 |
T13939 |
T13940 |
npadvmod |
Capn2,null |
R3420 |
T13940 |
T13941 |
amod |
null,embryos |
R3421 |
T13941 |
T13938 |
nsubj |
embryos,die |
R3422 |
T13716 |
T13717 |
det |
no,activity |
R3423 |
T13942 |
T13941 |
amod |
murine,embryos |
R3424 |
T13943 |
T13944 |
amod |
prior,to |
R3425 |
T13717 |
T13719 |
nsubjpass |
activity,observed |
R3426 |
T13944 |
T13938 |
prep |
to,die |
R3427 |
T13945 |
T13944 |
pobj |
implantation,to |
R3428 |
T13946 |
T13935 |
punct |
", ",affect |
R3429 |
T13718 |
T13717 |
compound |
calpain,activity |
R3430 |
T13947 |
T13948 |
amod |
homozygous,disruption |
R3431 |
T13948 |
T13935 |
nsubj |
disruption,affect |
R3432 |
T13949 |
T13948 |
prep |
of,disruption |
R3433 |
T13719 |
T13691 |
conj |
observed,provide |
R3434 |
T13950 |
T13951 |
det |
the,gene |
R3435 |
T13951 |
T13949 |
pobj |
gene,of |
R3436 |
T13720 |
T13719 |
auxpass |
was,observed |
R3437 |
T13952 |
T13953 |
nmod |
μ,calpain |
R3438 |
T13953 |
T13951 |
nmod |
calpain,gene |
R3439 |
T13954 |
T13953 |
punct |
-,calpain |
R3440 |
T13721 |
T13719 |
prep |
in,observed |
R3441 |
T13955 |
T13951 |
amod |
large,gene |
R3442 |
T13956 |
T13951 |
compound |
subunit,gene |
R3443 |
T13722 |
T13723 |
poss |
our,hands |
R3444 |
T13957 |
T13951 |
punct |
", ",gene |
R3445 |
T13958 |
T13951 |
appos |
Capn1,gene |
R3446 |
T13959 |
T13935 |
punct |
", ",affect |
R3447 |
T13960 |
T13935 |
aux |
did,affect |
R3448 |
T13961 |
T13935 |
neg |
not,affect |
R3449 |
T13962 |
T13963 |
det |
the,viability |
R3450 |
T14041 |
T14040 |
amod |
relative,amounts |
R3451 |
T13963 |
T13935 |
dobj |
viability,affect |
R3452 |
T13964 |
T13963 |
prep |
of,viability |
R3453 |
T13965 |
T13964 |
pobj |
mice,of |
R3454 |
T13966 |
T13967 |
punct |
[,33 |
R3455 |
T13967 |
T13935 |
parataxis |
33,affect |
R3456 |
T13968 |
T13967 |
punct |
],33 |
R3457 |
T14042 |
T14013 |
punct |
.,are |
R3458 |
T13969 |
T13935 |
punct |
.,affect |
R3459 |
T13971 |
T13972 |
det |
The,phenotype |
R3460 |
T14044 |
T14045 |
nsubj |
Platelets,contain |
R3461 |
T13972 |
T13974 |
nsubj |
phenotype,involved |
R3462 |
T13973 |
T13972 |
amod |
principal,phenotype |
R3463 |
T14046 |
T14044 |
cc |
and,Platelets |
R3464 |
T13975 |
T13972 |
acl |
observed,phenotype |
R3465 |
T13976 |
T13975 |
prep |
as,observed |
R3466 |
T13977 |
T13978 |
det |
a,result |
R3467 |
T13978 |
T13976 |
pobj |
result,as |
R3468 |
T14047 |
T14044 |
conj |
erythrocytes,Platelets |
R3469 |
T13979 |
T13978 |
prep |
of,result |
R3470 |
T13980 |
T13981 |
compound |
Capn1,deficiency |
R3471 |
T13981 |
T13979 |
pobj |
deficiency,of |
R3472 |
T14048 |
T14049 |
amod |
abundant,activity |
R3473 |
T13982 |
T13983 |
det |
a,disturbance |
R3474 |
T13983 |
T13974 |
dobj |
disturbance,involved |
R3475 |
T13984 |
T13983 |
prep |
in,disturbance |
R3476 |
T14049 |
T14045 |
dobj |
activity,contain |
R3477 |
T13985 |
T13986 |
compound |
platelet,function |
R3478 |
T13986 |
T13984 |
pobj |
function,in |
R3479 |
T13987 |
T13983 |
punct |
", ",disturbance |
R3480 |
T14050 |
T14051 |
compound |
μ,calpain |
R3481 |
T13988 |
T13989 |
advmod |
possibly,at |
R3482 |
T13989 |
T13983 |
prep |
at,disturbance |
R3483 |
T13990 |
T13991 |
det |
the,level |
R3484 |
T14051 |
T14049 |
compound |
calpain,activity |
R3485 |
T13991 |
T13989 |
pobj |
level,at |
R3486 |
T13992 |
T13991 |
prep |
of,level |
R3487 |
T13993 |
T13994 |
det |
the,phosphorylation |
R3488 |
T14052 |
T14051 |
punct |
-,calpain |
R3489 |
T13994 |
T13992 |
pobj |
phosphorylation,of |
R3490 |
T13995 |
T13994 |
compound |
tyrosine,phosphorylation |
R3491 |
T13996 |
T13994 |
prep |
of,phosphorylation |
R3492 |
T14053 |
T14045 |
punct |
", ",contain |
R3493 |
T13997 |
T13998 |
amod |
certain,proteins |
R3494 |
T13998 |
T13996 |
pobj |
proteins,of |
R3495 |
T13999 |
T13998 |
acl |
involved,proteins |
R3496 |
T14054 |
T14055 |
mark |
while,is |
R3497 |
T14055 |
T14045 |
advcl |
is,contain |
R3498 |
T14000 |
T13999 |
prep |
in,involved |
R3499 |
T14001 |
T14002 |
compound |
platelet,activation |
R3500 |
T14002 |
T14000 |
pobj |
activation,in |
R3501 |
T14003 |
T13974 |
punct |
.,involved |
R3502 |
T14056 |
T14057 |
compound |
m,calpain |
R3503 |
T14005 |
T14006 |
preconj |
Both,activities |
R3504 |
T14006 |
T14013 |
nsubj |
activities,are |
R3505 |
T14057 |
T14059 |
compound |
calpain,activity |
R3506 |
T14007 |
T14008 |
nmod |
μ,calpain |
R3507 |
T14008 |
T14006 |
compound |
calpain,activities |
R3508 |
T14009 |
T14007 |
punct |
-,μ |
R3509 |
T14058 |
T14057 |
punct |
-,calpain |
R3510 |
T14010 |
T14007 |
cc |
and,μ |
R3511 |
T14011 |
T14007 |
conj |
m,μ |
R3512 |
T14012 |
T14008 |
punct |
-,calpain |
R3513 |
T14059 |
T14055 |
nsubj |
activity,is |
R3514 |
T14014 |
T14013 |
acomp |
present,are |
R3515 |
T14060 |
T14061 |
advmod |
barely,detectable |
R3516 |
T14015 |
T14013 |
prep |
in,are |
R3517 |
T14061 |
T14055 |
acomp |
detectable,is |
R3518 |
T14062 |
T14045 |
punct |
.,contain |
R3519 |
T14016 |
T14017 |
amod |
most,cells |
R3520 |
T14017 |
T14015 |
pobj |
cells,in |
R3521 |
T14018 |
T14017 |
amod |
mammalian,cells |
R3522 |
T14064 |
T14065 |
nsubj |
Compensation,is |
R3523 |
T14019 |
T14013 |
punct |
", ",are |
R3524 |
T14020 |
T14021 |
mark |
although,provide |
R3525 |
T14066 |
T14064 |
prep |
for,Compensation |
R3526 |
T14021 |
T14013 |
advcl |
provide,are |
R3527 |
T14022 |
T14023 |
det |
the,data |
R3528 |
T14023 |
T14021 |
nsubj |
data,provide |
R3529 |
T14024 |
T14023 |
amod |
published,data |
R3530 |
T14067 |
T14068 |
compound |
μ,calpain |
R3531 |
T14025 |
T14023 |
punct |
", ",data |
R3532 |
T14026 |
T14023 |
prep |
owing,data |
R3533 |
T14027 |
T14026 |
prep |
to,owing |
R3534 |
T14068 |
T14070 |
compound |
calpain,deficiency |
R3535 |
T14028 |
T14027 |
pobj |
weaknesses,to |
R3536 |
T14029 |
T14028 |
prep |
in,weaknesses |
R3537 |
T14030 |
T14031 |
det |
the,methodology |
R3538 |
T14069 |
T14068 |
punct |
-,calpain |
R3539 |
T14031 |
T14029 |
pobj |
methodology,in |
R3540 |
T14032 |
T14031 |
amod |
available,methodology |
R3541 |
T14033 |
T14021 |
punct |
", ",provide |
R3542 |
T14070 |
T14066 |
pobj |
deficiency,for |
R3543 |
T14034 |
T14021 |
aux |
do,provide |
R3544 |
T14035 |
T14021 |
neg |
not,provide |
R3545 |
T14036 |
T14037 |
amod |
reliable,estimates |
R3546 |
T14071 |
T14064 |
prep |
by,Compensation |
R3547 |
T14037 |
T14021 |
dobj |
estimates,provide |
R3548 |
T14038 |
T14037 |
prep |
of,estimates |
R3549 |
T14039 |
T14040 |
poss |
their,amounts |
R3550 |
T14072 |
T14073 |
compound |
m,calpain |
R3551 |
T14040 |
T14038 |
pobj |
amounts,of |
R3552 |
T14073 |
T14071 |
pobj |
calpain,by |
R3553 |
T14074 |
T14073 |
punct |
-,calpain |
R3554 |
T14075 |
T14065 |
advmod |
therefore,is |
R3555 |
T14147 |
T14145 |
punct |
-,Capn2 |
R3556 |
T14076 |
T14077 |
advmod |
less,likely |
R3557 |
T14148 |
T14145 |
punct |
/,Capn2 |
R3558 |
T14149 |
T14145 |
punct |
-,Capn2 |
R3559 |
T14077 |
T14065 |
acomp |
likely,is |
R3560 |
T14150 |
T14151 |
mark |
that,is |
R3561 |
T14151 |
T14140 |
ccomp |
is,demonstrates |
R3562 |
T14078 |
T14077 |
prep |
in,likely |
R3563 |
T14152 |
T14153 |
compound |
m,calpain |
R3564 |
T14153 |
T14155 |
compound |
calpain,activity |
R3565 |
T14154 |
T14153 |
punct |
-,calpain |
R3566 |
T14079 |
T14078 |
pobj |
platelets,in |
R3567 |
T14155 |
T14151 |
nsubj |
activity,is |
R3568 |
T14156 |
T14151 |
acomp |
essential,is |
R3569 |
T14157 |
T14156 |
prep |
for,essential |
R3570 |
T14080 |
T14079 |
cc |
and,platelets |
R3571 |
T14158 |
T14159 |
amod |
embryonic,development |
R3572 |
T14159 |
T14157 |
pobj |
development,for |
R3573 |
T14160 |
T14159 |
prep |
beyond,development |
R3574 |
T14081 |
T14079 |
conj |
erythrocytes,platelets |
R3575 |
T14161 |
T14162 |
det |
the,stage |
R3576 |
T14162 |
T14160 |
pobj |
stage,beyond |
R3577 |
T14082 |
T14077 |
prep |
than,likely |
R3578 |
T14163 |
T14164 |
nummod |
8,cell |
R3579 |
T14164 |
T14162 |
compound |
cell,stage |
R3580 |
T14165 |
T14164 |
punct |
-,cell |
R3581 |
T14083 |
T14082 |
prep |
in,than |
R3582 |
T14166 |
T14140 |
punct |
.,demonstrates |
R3583 |
T14168 |
T14169 |
nsubj |
It,follows |
R3584 |
T14084 |
T14085 |
amod |
other,types |
R3585 |
T14170 |
T14171 |
mark |
that,are |
R3586 |
T14171 |
T14169 |
ccomp |
are,follows |
R3587 |
T14085 |
T14083 |
pobj |
types,in |
R3588 |
T14172 |
T14173 |
advmod |
at,least |
R3589 |
T14173 |
T14174 |
advmod |
least,functions |
R3590 |
T14086 |
T14085 |
compound |
cell,types |
R3591 |
T14174 |
T14171 |
nsubj |
functions,are |
R3592 |
T14175 |
T14174 |
det |
some,functions |
R3593 |
T14176 |
T14174 |
prep |
of,functions |
R3594 |
T14087 |
T14065 |
punct |
.,is |
R3595 |
T14177 |
T14178 |
compound |
μ,calpain |
R3596 |
T14178 |
T14176 |
pobj |
calpain,of |
R3597 |
T14179 |
T14178 |
punct |
-,calpain |
R3598 |
T14089 |
T14090 |
prep |
As,is |
R3599 |
T14180 |
T14178 |
cc |
and,calpain |
R3600 |
T14181 |
T14182 |
compound |
m,calpain |
R3601 |
T14182 |
T14178 |
conj |
calpain,calpain |
R3602 |
T14183 |
T14182 |
punct |
-,calpain |
R3603 |
T14184 |
T14171 |
acomp |
distinct,are |
R3604 |
T14185 |
T14169 |
punct |
.,follows |
R3605 |
T14091 |
T14092 |
det |
a,result |
R3606 |
T14187 |
T14188 |
det |
The,cause |
R3607 |
T14092 |
T14089 |
pobj |
result,As |
R3608 |
T14188 |
T14190 |
nsubjpass |
cause,clarified |
R3609 |
T14189 |
T14188 |
amod |
underlying,cause |
R3610 |
T14093 |
T14090 |
punct |
", ",is |
R3611 |
T14191 |
T14188 |
prep |
of,cause |
R3612 |
T14192 |
T14193 |
det |
the,lethality |
R3613 |
T14193 |
T14191 |
pobj |
lethality,of |
R3614 |
T14094 |
T14090 |
nsubj |
it,is |
R3615 |
T14194 |
T14193 |
compound |
preimplantation,lethality |
R3616 |
T14195 |
T14188 |
prep |
in,cause |
R3617 |
T14196 |
T14197 |
nmod |
Capn2,embryos |
R3618 |
T14095 |
T14090 |
neg |
not,is |
R3619 |
T14197 |
T14195 |
pobj |
embryos,in |
R3620 |
T14198 |
T14196 |
punct |
-,Capn2 |
R3621 |
T14096 |
T14090 |
acomp |
possible,is |
R3622 |
T14199 |
T14196 |
punct |
/,Capn2 |
R3623 |
T14200 |
T14196 |
punct |
-,Capn2 |
R3624 |
T14201 |
T14190 |
aux |
has,clarified |
R3625 |
T14097 |
T14098 |
aux |
to,determine |
R3626 |
T14202 |
T14190 |
neg |
not,clarified |
R3627 |
T14203 |
T14190 |
advmod |
yet,clarified |
R3628 |
T14204 |
T14190 |
auxpass |
been,clarified |
R3629 |
T14098 |
T14090 |
xcomp |
determine,is |
R3630 |
T14205 |
T14190 |
punct |
.,clarified |
R3631 |
T14099 |
T14100 |
mark |
whether,is |
R3632 |
T14207 |
T14208 |
det |
The,embryos |
R3633 |
T14208 |
T14212 |
nsubj |
embryos,present |
R3634 |
T14209 |
T14208 |
nummod |
two,embryos |
R3635 |
T14100 |
T14098 |
advcl |
is,determine |
R3636 |
T14210 |
T14211 |
amod |
homozygous,null |
R3637 |
T14211 |
T14208 |
amod |
null,embryos |
R3638 |
T14101 |
T14102 |
det |
the,absence |
R3639 |
T14213 |
T14208 |
acl |
identified,embryos |
R3640 |
T14214 |
T14213 |
prep |
at,identified |
R3641 |
T14215 |
T14216 |
det |
the,stage |
R3642 |
T14102 |
T14100 |
nsubj |
absence,is |
R3643 |
T14216 |
T14214 |
pobj |
stage,at |
R3644 |
T14217 |
T14218 |
nummod |
8,cell |
R3645 |
T14218 |
T14216 |
compound |
cell,stage |
R3646 |
T14103 |
T14102 |
prep |
of,absence |
R3647 |
T14219 |
T14218 |
punct |
-,cell |
R3648 |
T14220 |
T14212 |
aux |
did,present |
R3649 |
T14221 |
T14212 |
neg |
not,present |
R3650 |
T14104 |
T14105 |
amod |
marked,phenotype |
R3651 |
T14222 |
T14223 |
det |
any,defects |
R3652 |
T14223 |
T14212 |
dobj |
defects,present |
R3653 |
T14105 |
T14103 |
pobj |
phenotype,of |
R3654 |
T14224 |
T14223 |
amod |
obvious,defects |
R3655 |
T14225 |
T14223 |
amod |
morphological,defects |
R3656 |
T14226 |
T14212 |
punct |
.,present |
R3657 |
T14228 |
T14229 |
advmod |
However,is |
R3658 |
T14106 |
T14102 |
prep |
in,absence |
R3659 |
T14230 |
T14229 |
punct |
", ",is |
R3660 |
T14231 |
T14232 |
det |
the,fact |
R3661 |
T14232 |
T14229 |
nsubj |
fact,is |
R3662 |
T14107 |
T14108 |
nmod |
Capn1,animals |
R3663 |
T14233 |
T14234 |
mark |
that,proved |
R3664 |
T14234 |
T14232 |
acl |
proved,fact |
R3665 |
T14235 |
T14236 |
advmod |
only,embryos |
R3666 |
T14108 |
T14106 |
pobj |
animals,in |
R3667 |
T14236 |
T14234 |
nsubj |
embryos,proved |
R3668 |
T14237 |
T14238 |
quantmod |
two,90 |
R3669 |
T14238 |
T14236 |
nummod |
90,embryos |
R3670 |
T14109 |
T14107 |
punct |
-,Capn1 |
R3671 |
T14239 |
T14238 |
quantmod |
out,90 |
R3672 |
T14240 |
T14238 |
quantmod |
of,90 |
R3673 |
T14241 |
T14242 |
advmod |
successfully,genotyped |
R3674 |
T14110 |
T14107 |
punct |
/,Capn1 |
R3675 |
T14242 |
T14236 |
amod |
genotyped,embryos |
R3676 |
T14243 |
T14236 |
amod |
pre-implantation,embryos |
R3677 |
T14244 |
T14245 |
aux |
to,be |
R3678 |
T14111 |
T14107 |
punct |
-,Capn1 |
R3679 |
T14245 |
T14234 |
xcomp |
be,proved |
R3680 |
T14246 |
T14245 |
attr |
Capn2,be |
R3681 |
T14247 |
T14246 |
punct |
-,Capn2 |
R3682 |
T14112 |
T14100 |
prep |
due,is |
R3683 |
T14248 |
T14246 |
punct |
/,Capn2 |
R3684 |
T14249 |
T14246 |
punct |
-,Capn2 |
R3685 |
T14113 |
T14112 |
pcomp |
to,due |
R3686 |
T14250 |
T14229 |
prep |
in,is |
R3687 |
T14251 |
T14250 |
pobj |
itself,in |
R3688 |
T14252 |
T14229 |
acomp |
revealing,is |
R3689 |
T14114 |
T14115 |
det |
a,affect |
R3690 |
T14115 |
T14112 |
pobj |
affect,due |
R3691 |
T14116 |
T14115 |
amod |
compensatory,affect |
R3692 |
T14253 |
T14229 |
punct |
.,is |
R3693 |
T14117 |
T14115 |
prep |
by,affect |
R3694 |
T14255 |
T14256 |
compound |
Preimplantation,lethality |
R3695 |
T14256 |
T14257 |
nsubjpass |
lethality,attributed |
R3696 |
T14118 |
T14119 |
det |
the,activity |
R3697 |
T14258 |
T14256 |
acl |
resulting,lethality |
R3698 |
T14259 |
T14258 |
prep |
from,resulting |
R3699 |
T14119 |
T14117 |
pobj |
activity,by |
R3700 |
T14260 |
T14261 |
compound |
gene,knockouts |
R3701 |
T14261 |
T14259 |
pobj |
knockouts,from |
R3702 |
T14120 |
T14119 |
amod |
remaining,activity |
R3703 |
T14262 |
T14257 |
aux |
can,attributed |
R3704 |
T14263 |
T14257 |
advmod |
typically,attributed |
R3705 |
T14264 |
T14257 |
auxpass |
be,attributed |
R3706 |
T14265 |
T14257 |
prep |
to,attributed |
R3707 |
T14266 |
T14267 |
nummod |
two,causes |
R3708 |
T14267 |
T14265 |
pobj |
causes,to |
R3709 |
T14121 |
T14122 |
compound |
m,calpain |
R3710 |
T14268 |
T14267 |
amod |
general,causes |
R3711 |
T14269 |
T14257 |
punct |
.,attributed |
R3712 |
T14122 |
T14119 |
compound |
calpain,activity |
R3713 |
T14271 |
T14272 |
prep |
In,incurred |
R3714 |
T14273 |
T14274 |
det |
some,cases |
R3715 |
T14123 |
T14122 |
punct |
-,calpain |
R3716 |
T14274 |
T14271 |
pobj |
cases,In |
R3717 |
T14275 |
T14272 |
punct |
", ",incurred |
R3718 |
T14124 |
T14100 |
punct |
", ",is |
R3719 |
T14276 |
T14272 |
nsubjpass |
defects,incurred |
R3720 |
T14277 |
T14272 |
auxpass |
are,incurred |
R3721 |
T14278 |
T14272 |
prep |
in,incurred |
R3722 |
T14125 |
T14100 |
cc |
or,is |
R3723 |
T14126 |
T14127 |
mark |
whether,are |
R3724 |
T14279 |
T14280 |
det |
the,program |
R3725 |
T14127 |
T14100 |
conj |
are,is |
R3726 |
T14280 |
T14278 |
pobj |
program,in |
R3727 |
T14281 |
T14280 |
amod |
embryonic,program |
R3728 |
T14282 |
T14280 |
compound |
differentiation,program |
R3729 |
T14128 |
T14129 |
det |
the,functions |
R3730 |
T14283 |
T14284 |
dep |
which,observed |
R3731 |
T14284 |
T14280 |
relcl |
observed,program |
R3732 |
T14285 |
T14284 |
aux |
can,observed |
R3733 |
T14129 |
T14127 |
nsubj |
functions,are |
R3734 |
T14286 |
T14284 |
advmod |
often,observed |
R3735 |
T14287 |
T14284 |
auxpass |
be,observed |
R3736 |
T14130 |
T14129 |
prep |
of,functions |
R3737 |
T14288 |
T14284 |
advmod |
morphologically,observed |
R3738 |
T14289 |
T14290 |
punct |
[,38 |
R3739 |
T14131 |
T14132 |
compound |
μ,calpain |
R3740 |
T14290 |
T14284 |
parataxis |
38,observed |
R3741 |
T14291 |
T14290 |
punct |
],38 |
R3742 |
T14292 |
T14272 |
punct |
.,incurred |
R3743 |
T14132 |
T14130 |
pobj |
calpain,of |
R3744 |
T14294 |
T14295 |
amod |
Null,embryos |
R3745 |
T14295 |
T14296 |
nsubj |
embryos,survive |
R3746 |
T14133 |
T14132 |
punct |
-,calpain |
R3747 |
T14297 |
T14295 |
prep |
of,embryos |
R3748 |
T14298 |
T14299 |
det |
this,type |
R3749 |
T14134 |
T14127 |
advmod |
simply,are |
R3750 |
T14299 |
T14297 |
pobj |
type,of |
R3751 |
T14300 |
T14296 |
advmod |
often,survive |
R3752 |
T14135 |
T14127 |
neg |
not,are |
R3753 |
T14301 |
T14296 |
prep |
beyond,survive |
R3754 |
T14302 |
T14303 |
det |
the,stage |
R3755 |
T14303 |
T14301 |
pobj |
stage,beyond |
R3756 |
T14136 |
T14127 |
acomp |
essential,are |
R3757 |
T14304 |
T14303 |
compound |
morula,stage |
R3758 |
T14305 |
T14296 |
punct |
", ",survive |
R3759 |
T14306 |
T14296 |
cc |
and,survive |
R3760 |
T14307 |
T14308 |
det |
a,distribution |
R3761 |
T14308 |
T14310 |
nsubjpass |
distribution,noted |
R3762 |
T14137 |
T14090 |
punct |
.,is |
R3763 |
T14309 |
T14308 |
amod |
Mendelian,distribution |
R3764 |
T14310 |
T14296 |
conj |
noted,survive |
R3765 |
T14311 |
T14308 |
prep |
of,distribution |
R3766 |
T14139 |
T14140 |
prep |
In,demonstrates |
R3767 |
T14312 |
T14313 |
amod |
embryonic,genotypes |
R3768 |
T14313 |
T14311 |
pobj |
genotypes,of |
R3769 |
T14314 |
T14310 |
auxpass |
is,noted |
R3770 |
T14315 |
T14310 |
advmod |
usually,noted |
R3771 |
T14141 |
T14139 |
pobj |
contrast,In |
R3772 |
T14316 |
T14310 |
punct |
.,noted |
R3773 |
T14318 |
T14319 |
prep |
In,thought |
R3774 |
T14142 |
T14140 |
punct |
", ",demonstrates |
R3775 |
T14320 |
T14321 |
amod |
other,cases |
R3776 |
T14321 |
T14318 |
pobj |
cases,In |
R3777 |
T14322 |
T14319 |
punct |
", ",thought |
R3778 |
T14143 |
T14140 |
nsubj |
lethality,demonstrates |
R3779 |
T14323 |
T14319 |
advmod |
however,thought |
R3780 |
T14324 |
T14319 |
punct |
", ",thought |
R3781 |
T14144 |
T14143 |
prep |
in,lethality |
R3782 |
T14325 |
T14326 |
det |
the,disruption |
R3783 |
T14326 |
T14319 |
nsubjpass |
disruption,thought |
R3784 |
T14327 |
T14326 |
compound |
gene,disruption |
R3785 |
T14145 |
T14146 |
nmod |
Capn2,embryos |
R3786 |
T14328 |
T14319 |
auxpass |
is,thought |
R3787 |
T14329 |
T14330 |
aux |
to,compromise |
R3788 |
T14146 |
T14144 |
pobj |
embryos,in |
R3789 |
T14330 |
T14319 |
xcomp |
compromise,thought |
R3790 |
T14331 |
T14330 |
advmod |
fatally,compromise |
R3791 |
T14332 |
T14333 |
det |
the,viability |
R3792 |
T14359 |
T14350 |
auxpass |
are,observed |
R3793 |
T14333 |
T14330 |
dobj |
viability,compromise |
R3794 |
T14334 |
T14333 |
prep |
of,viability |
R3795 |
T14335 |
T14334 |
pobj |
cells,of |
R3796 |
T14336 |
T14335 |
prep |
in,cells |
R3797 |
T14360 |
T14350 |
advmod |
ever,observed |
R3798 |
T14337 |
T14338 |
det |
the,embryo |
R3799 |
T14338 |
T14336 |
pobj |
embryo,in |
R3800 |
T14339 |
T14338 |
amod |
early,embryo |
R3801 |
T14361 |
T14350 |
cc |
and,observed |
R3802 |
T14340 |
T14341 |
punct |
[,39 |
R3803 |
T14341 |
T14330 |
parataxis |
39,compromise |
R3804 |
T14342 |
T14341 |
dep |
3,39 |
R3805 |
T14362 |
T14363 |
amod |
homozygous,cells |
R3806 |
T14343 |
T14341 |
punct |
",",39 |
R3807 |
T14344 |
T14345 |
punct |
-,43 |
R3808 |
T14345 |
T14341 |
prep |
43,39 |
R3809 |
T14363 |
T14366 |
nsubjpass |
cells,isolated |
R3810 |
T14346 |
T14341 |
punct |
],39 |
R3811 |
T14347 |
T14319 |
punct |
.,thought |
R3812 |
T14349 |
T14350 |
prep |
In,observed |
R3813 |
T14364 |
T14363 |
compound |
mutant,cells |
R3814 |
T14351 |
T14352 |
det |
these,instances |
R3815 |
T14352 |
T14349 |
pobj |
instances,In |
R3816 |
T14353 |
T14350 |
punct |
", ",observed |
R3817 |
T14365 |
T14363 |
compound |
ES,cells |
R3818 |
T14354 |
T14355 |
advmod |
only,embryos |
R3819 |
T14355 |
T14350 |
nsubjpass |
embryos,observed |
R3820 |
T14356 |
T14355 |
det |
a,embryos |
R3821 |
T14366 |
T14350 |
conj |
isolated,observed |
R3822 |
T14357 |
T14355 |
amod |
few,embryos |
R3823 |
T14358 |
T14355 |
amod |
null,embryos |
R3824 |
T14367 |
T14366 |
aux |
could,isolated |
R3825 |
T14368 |
T14366 |
neg |
not,isolated |
R3826 |
T14369 |
T14366 |
auxpass |
be,isolated |
R3827 |
T14370 |
T14366 |
punct |
.,isolated |
R3828 |
T14372 |
T14373 |
det |
The,knockout |
R3829 |
T14465 |
T14467 |
compound |
calpain,involvement |
R3830 |
T14373 |
T14375 |
nsubj |
knockout,fits |
R3831 |
T14466 |
T14465 |
punct |
-,calpain |
R3832 |
T14467 |
T14463 |
pobj |
involvement,of |
R3833 |
T14468 |
T14467 |
prep |
in,involvement |
R3834 |
T14374 |
T14373 |
compound |
Capn2,knockout |
R3835 |
T14469 |
T14470 |
compound |
cell,proliferation |
R3836 |
T14470 |
T14468 |
pobj |
proliferation,in |
R3837 |
T14376 |
T14375 |
prep |
into,fits |
R3838 |
T14471 |
T14467 |
prep |
in,involvement |
R3839 |
T14472 |
T14473 |
amod |
certain,circumstances |
R3840 |
T14473 |
T14471 |
pobj |
circumstances,in |
R3841 |
T14474 |
T14462 |
punct |
", ",reports |
R3842 |
T14377 |
T14378 |
det |
the,category |
R3843 |
T14475 |
T14462 |
prep |
including,reports |
R3844 |
T14476 |
T14475 |
pobj |
reports,including |
R3845 |
T14477 |
T14476 |
prep |
of,reports |
R3846 |
T14378 |
T14376 |
pobj |
category,into |
R3847 |
T14478 |
T14479 |
poss |
its,involvement |
R3848 |
T14479 |
T14477 |
pobj |
involvement,of |
R3849 |
T14480 |
T14479 |
prep |
in,involvement |
R3850 |
T14379 |
T14378 |
amod |
latter,category |
R3851 |
T14481 |
T14482 |
compound |
chromosome,segregation |
R3852 |
T14482 |
T14480 |
pobj |
segregation,in |
R3853 |
T14380 |
T14375 |
punct |
.,fits |
R3854 |
T14483 |
T14482 |
prep |
during,segregation |
R3855 |
T14484 |
T14483 |
pobj |
mitosis,during |
R3856 |
T14485 |
T14486 |
punct |
[,44 |
R3857 |
T14382 |
T14383 |
mark |
If,is |
R3858 |
T14486 |
T14484 |
parataxis |
44,mitosis |
R3859 |
T14487 |
T14486 |
punct |
],44 |
R3860 |
T14488 |
T14489 |
advmod |
as,as |
R3861 |
T14489 |
T14483 |
cc |
as,during |
R3862 |
T14490 |
T14489 |
advmod |
well,as |
R3863 |
T14491 |
T14483 |
conj |
during,during |
R3864 |
T14492 |
T14491 |
pobj |
meiosis,during |
R3865 |
T14383 |
T14385 |
advcl |
is,is |
R3866 |
T14493 |
T14494 |
punct |
[,45 |
R3867 |
T14494 |
T14492 |
parataxis |
45,meiosis |
R3868 |
T14495 |
T14494 |
punct |
],45 |
R3869 |
T14384 |
T14383 |
nsubj |
it,is |
R3870 |
T14496 |
T14459 |
punct |
.,been |
R3871 |
T14386 |
T14383 |
acomp |
true,is |
R3872 |
T14498 |
T14499 |
nsubj |
Defects,contribute |
R3873 |
T14500 |
T14498 |
prep |
in,Defects |
R3874 |
T14501 |
T14500 |
pobj |
migration,in |
R3875 |
T14387 |
T14388 |
mark |
that,is |
R3876 |
T14502 |
T14498 |
punct |
", ",Defects |
R3877 |
T14503 |
T14498 |
acl |
reported,Defects |
R3878 |
T14504 |
T14503 |
prep |
in,reported |
R3879 |
T14388 |
T14383 |
ccomp |
is,is |
R3880 |
T14505 |
T14506 |
nmod |
Capn4,cells |
R3881 |
T14506 |
T14504 |
pobj |
cells,in |
R3882 |
T14507 |
T14505 |
punct |
-,Capn4 |
R3883 |
T14389 |
T14390 |
compound |
m,calpain |
R3884 |
T14508 |
T14505 |
punct |
/,Capn4 |
R3885 |
T14509 |
T14505 |
punct |
-,Capn4 |
R3886 |
T14390 |
T14388 |
nsubj |
calpain,is |
R3887 |
T14510 |
T14511 |
punct |
[,9 |
R3888 |
T14511 |
T14503 |
parataxis |
9,reported |
R3889 |
T14512 |
T14511 |
punct |
],9 |
R3890 |
T14391 |
T14390 |
punct |
-,calpain |
R3891 |
T14513 |
T14499 |
punct |
", ",contribute |
R3892 |
T14514 |
T14499 |
aux |
could,contribute |
R3893 |
T14515 |
T14499 |
prep |
to,contribute |
R3894 |
T14392 |
T14388 |
acomp |
essential,is |
R3895 |
T14516 |
T14517 |
amod |
failed,development |
R3896 |
T14393 |
T14392 |
prep |
for,essential |
R3897 |
T14517 |
T14515 |
pobj |
development,to |
R3898 |
T14518 |
T14517 |
amod |
embryonic,development |
R3899 |
T14519 |
T14499 |
punct |
.,contribute |
R3900 |
T14394 |
T14395 |
det |
some,aspect |
R3901 |
T14521 |
T14522 |
det |
The,association |
R3902 |
T14522 |
T14523 |
nsubjpass |
association,noted |
R3903 |
T14395 |
T14393 |
pobj |
aspect,for |
R3904 |
T14524 |
T14522 |
prep |
between,association |
R3905 |
T14396 |
T14395 |
prep |
of,aspect |
R3906 |
T14525 |
T14524 |
pobj |
calpain,between |
R3907 |
T14526 |
T14525 |
cc |
and,calpain |
R3908 |
T14397 |
T14398 |
compound |
cell,viability |
R3909 |
T14527 |
T14528 |
compound |
cell,viability |
R3910 |
T14528 |
T14525 |
conj |
viability,calpain |
R3911 |
T14529 |
T14523 |
aux |
has,noted |
R3912 |
T14398 |
T14396 |
pobj |
viability,of |
R3913 |
T14530 |
T14523 |
auxpass |
been,noted |
R3914 |
T14531 |
T14523 |
prep |
in,noted |
R3915 |
T14532 |
T14533 |
nmod |
Capn4,MEFs |
R3916 |
T14533 |
T14531 |
pobj |
MEFs,in |
R3917 |
T14534 |
T14532 |
punct |
-,Capn4 |
R3918 |
T14535 |
T14532 |
punct |
/,Capn4 |
R3919 |
T14536 |
T14532 |
punct |
-,Capn4 |
R3920 |
T14399 |
T14385 |
punct |
", ",is |
R3921 |
T14537 |
T14533 |
cc |
and,MEFs |
R3922 |
T14538 |
T14539 |
amod |
other,lines |
R3923 |
T14400 |
T14401 |
det |
the,survival |
R3924 |
T14401 |
T14385 |
nsubj |
survival,is |
R3925 |
T14539 |
T14533 |
conj |
lines,MEFs |
R3926 |
T14540 |
T14539 |
compound |
cell,lines |
R3927 |
T14541 |
T14523 |
punct |
", ",noted |
R3928 |
T14402 |
T14401 |
prep |
of,survival |
R3929 |
T14542 |
T14543 |
mark |
although,distinguish |
R3930 |
T14543 |
T14523 |
advcl |
distinguish,noted |
R3931 |
T14403 |
T14404 |
det |
a,embryos |
R3932 |
T14544 |
T14545 |
det |
the,work |
R3933 |
T14545 |
T14543 |
nsubj |
work,distinguish |
R3934 |
T14404 |
T14402 |
pobj |
embryos,of |
R3935 |
T14546 |
T14545 |
amod |
reported,work |
R3936 |
T14547 |
T14543 |
advmod |
frequently,distinguish |
R3937 |
T14405 |
T14404 |
amod |
few,embryos |
R3938 |
T14548 |
T14543 |
aux |
did,distinguish |
R3939 |
T14549 |
T14543 |
neg |
not,distinguish |
R3940 |
T14550 |
T14543 |
prep |
between,distinguish |
R3941 |
T14406 |
T14404 |
nmod |
Capn2,embryos |
R3942 |
T14551 |
T14552 |
nmod |
μ,calpain |
R3943 |
T14552 |
T14550 |
pobj |
calpain,between |
R3944 |
T14407 |
T14406 |
punct |
-,Capn2 |
R3945 |
T14553 |
T14551 |
punct |
-,μ |
R3946 |
T14554 |
T14551 |
cc |
and,μ |
R3947 |
T14408 |
T14406 |
punct |
/,Capn2 |
R3948 |
T14555 |
T14551 |
conj |
m,μ |
R3949 |
T14556 |
T14552 |
punct |
-,calpain |
R3950 |
T14557 |
T14543 |
punct |
", ",distinguish |
R3951 |
T14409 |
T14406 |
punct |
-,Capn2 |
R3952 |
T14558 |
T14543 |
cc |
and,distinguish |
R3953 |
T14559 |
T14560 |
aux |
did,show |
R3954 |
T14410 |
T14401 |
prep |
to,survival |
R3955 |
T14560 |
T14543 |
conj |
show,distinguish |
R3956 |
T14561 |
T14560 |
neg |
not,show |
R3957 |
T14562 |
T14563 |
mark |
that,was |
R3958 |
T14411 |
T14412 |
det |
the,stage |
R3959 |
T14563 |
T14560 |
ccomp |
was,show |
R3960 |
T14564 |
T14563 |
nsubj |
calpain,was |
R3961 |
T14565 |
T14566 |
advmod |
strictly,essential |
R3962 |
T14412 |
T14410 |
pobj |
stage,to |
R3963 |
T14566 |
T14563 |
acomp |
essential,was |
R3964 |
T14567 |
T14568 |
punct |
[,6 |
R3965 |
T14568 |
T14560 |
parataxis |
6,show |
R3966 |
T14569 |
T14568 |
nummod |
5,6 |
R3967 |
T14413 |
T14414 |
nummod |
8,cell |
R3968 |
T14414 |
T14412 |
compound |
cell,stage |
R3969 |
T14570 |
T14568 |
punct |
",",6 |
R3970 |
T14571 |
T14568 |
punct |
],6 |
R3971 |
T14572 |
T14523 |
punct |
.,noted |
R3972 |
T14415 |
T14414 |
punct |
-,cell |
R3973 |
T14574 |
T14575 |
prep |
In,shown |
R3974 |
T14416 |
T14417 |
advmod |
most,likely |
R3975 |
T14576 |
T14577 |
preconj |
both,cells |
R3976 |
T14577 |
T14574 |
pobj |
cells,In |
R3977 |
T14417 |
T14418 |
advmod |
likely,due |
R3978 |
T14578 |
T14577 |
compound |
CHO,cells |
R3979 |
T14579 |
T14577 |
cc |
and,cells |
R3980 |
T14580 |
T14581 |
nmod |
Capn4,MEFs |
R3981 |
T14418 |
T14385 |
prep |
due,is |
R3982 |
T14581 |
T14577 |
conj |
MEFs,cells |
R3983 |
T14582 |
T14580 |
punct |
-,Capn4 |
R3984 |
T14583 |
T14580 |
punct |
/,Capn4 |
R3985 |
T14419 |
T14418 |
pcomp |
to,due |
R3986 |
T14584 |
T14580 |
punct |
-,Capn4 |
R3987 |
T14585 |
T14575 |
punct |
", ",shown |
R3988 |
T14420 |
T14421 |
det |
the,persistence |
R3989 |
T14586 |
T14575 |
nsubjpass |
calpain,shown |
R3990 |
T14587 |
T14575 |
auxpass |
was,shown |
R3991 |
T14588 |
T14589 |
aux |
to,influence |
R3992 |
T14421 |
T14418 |
pobj |
persistence,due |
R3993 |
T14589 |
T14575 |
xcomp |
influence,shown |
R3994 |
T14590 |
T14591 |
compound |
cell,proliferation |
R3995 |
T14591 |
T14589 |
dobj |
proliferation,influence |
R3996 |
T14422 |
T14421 |
prep |
of,persistence |
R3997 |
T14592 |
T14589 |
punct |
", ",influence |
R3998 |
T14593 |
T14594 |
cc |
but,at |
R3999 |
T14594 |
T14589 |
prep |
at,influence |
R4000 |
T14423 |
T14424 |
det |
some,calpain |
R4001 |
T14595 |
T14594 |
advmod |
only,at |
R4002 |
T14596 |
T14597 |
advmod |
very,low |
R4003 |
T14597 |
T14598 |
amod |
low,densities |
R4004 |
T14424 |
T14422 |
pobj |
calpain,of |
R4005 |
T14598 |
T14594 |
pobj |
densities,at |
R4006 |
T14599 |
T14598 |
compound |
cell,densities |
R4007 |
T14600 |
T14601 |
punct |
[,5 |
R4008 |
T14425 |
T14424 |
amod |
maternal,calpain |
R4009 |
T14601 |
T14589 |
parataxis |
5,influence |
R4010 |
T14602 |
T14601 |
punct |
],5 |
R4011 |
T14426 |
T14424 |
compound |
m,calpain |
R4012 |
T14603 |
T14575 |
punct |
.,shown |
R4013 |
T14605 |
T14606 |
nsubjpass |
Calpain,associated |
R4014 |
T14427 |
T14424 |
punct |
-,calpain |
R4015 |
T14607 |
T14606 |
aux |
has,associated |
R4016 |
T14428 |
T14424 |
appos |
mRNA,calpain |
R4017 |
T14608 |
T14606 |
advmod |
also,associated |
R4018 |
T14609 |
T14606 |
auxpass |
been,associated |
R4019 |
T14610 |
T14606 |
prep |
with,associated |
R4020 |
T14611 |
T14610 |
pobj |
progression,with |
R4021 |
T14612 |
T14611 |
prep |
through,progression |
R4022 |
T14429 |
T14428 |
cc |
and,mRNA |
R4023 |
T14613 |
T14614 |
det |
the,stage |
R4024 |
T14614 |
T14612 |
pobj |
stage,through |
R4025 |
T14615 |
T14614 |
compound |
G1,stage |
R4026 |
T14430 |
T14429 |
punct |
/,and |
R4027 |
T14616 |
T14614 |
prep |
of,stage |
R4028 |
T14617 |
T14618 |
det |
the,cycle |
R4029 |
T14431 |
T14429 |
cc |
or,and |
R4030 |
T14618 |
T14616 |
pobj |
cycle,of |
R4031 |
T14619 |
T14618 |
compound |
cell,cycle |
R4032 |
T14620 |
T14621 |
punct |
[,6 |
R4033 |
T14432 |
T14428 |
conj |
protein,mRNA |
R4034 |
T14621 |
T14606 |
parataxis |
6,associated |
R4035 |
T14622 |
T14621 |
punct |
],6 |
R4036 |
T14623 |
T14606 |
punct |
.,associated |
R4037 |
T14433 |
T14421 |
prep |
through,persistence |
R4038 |
T14434 |
T14435 |
quantmod |
2,3 |
R4039 |
T14625 |
T14626 |
advmod |
Furthermore,reputed |
R4040 |
T14435 |
T14437 |
nummod |
3,divisions |
R4041 |
T14627 |
T14626 |
punct |
", ",reputed |
R4042 |
T14436 |
T14435 |
punct |
–,3 |
R4043 |
T14628 |
T14626 |
nsubjpass |
some,reputed |
R4044 |
T14629 |
T14628 |
prep |
of,some |
R4045 |
T14630 |
T14631 |
det |
the,proteins |
R4046 |
T14437 |
T14433 |
pobj |
divisions,through |
R4047 |
T14631 |
T14629 |
pobj |
proteins,of |
R4048 |
T14632 |
T14631 |
acl |
known,proteins |
R4049 |
T14438 |
T14437 |
compound |
cell,divisions |
R4050 |
T14633 |
T14634 |
aux |
to,involved |
R4051 |
T14634 |
T14632 |
xcomp |
involved,known |
R4052 |
T14635 |
T14634 |
auxpass |
be,involved |
R4053 |
T14439 |
T14385 |
punct |
.,is |
R4054 |
T14636 |
T14634 |
prep |
in,involved |
R4055 |
T14637 |
T14638 |
compound |
cell,cycle |
R4056 |
T14638 |
T14639 |
compound |
cycle,progression |
R4057 |
T14441 |
T14442 |
det |
The,reasons |
R4058 |
T14639 |
T14636 |
pobj |
progression,in |
R4059 |
T14640 |
T14631 |
punct |
", ",proteins |
R4060 |
T14641 |
T14642 |
amod |
such,as |
R4061 |
T14642 |
T14631 |
prep |
as,proteins |
R4062 |
T14442 |
T14444 |
nsubj |
reasons,are |
R4063 |
T14643 |
T14642 |
pobj |
p53,as |
R4064 |
T14644 |
T14643 |
punct |
", ",p53 |
R4065 |
T14645 |
T14643 |
conj |
p107,p53 |
R4066 |
T14443 |
T14442 |
amod |
mechanistic,reasons |
R4067 |
T14646 |
T14645 |
punct |
", ",p107 |
R4068 |
T14647 |
T14648 |
compound |
cyclin,D1 |
R4069 |
T14445 |
T14442 |
prep |
for,reasons |
R4070 |
T14648 |
T14645 |
conj |
D1,p107 |
R4071 |
T14649 |
T14648 |
punct |
", ",D1 |
R4072 |
T14650 |
T14648 |
cc |
and,D1 |
R4073 |
T14651 |
T14648 |
conj |
p27kip1,D1 |
R4074 |
T14652 |
T14626 |
auxpass |
are,reputed |
R4075 |
T14653 |
T14654 |
aux |
to,be |
R4076 |
T14446 |
T14445 |
pobj |
lethality,for |
R4077 |
T14654 |
T14626 |
xcomp |
be,reputed |
R4078 |
T14655 |
T14656 |
compound |
calpain,substrates |
R4079 |
T14656 |
T14654 |
attr |
substrates,be |
R4080 |
T14447 |
T14442 |
prep |
in,reasons |
R4081 |
T14657 |
T14658 |
punct |
[,3 |
R4082 |
T14658 |
T14654 |
parataxis |
3,be |
R4083 |
T14659 |
T14658 |
punct |
],3 |
R4084 |
T14448 |
T14449 |
det |
the,absence |
R4085 |
T14660 |
T14626 |
punct |
.,reputed |
R4086 |
T14662 |
T14663 |
compound |
Sperm,binding |
R4087 |
T14449 |
T14447 |
pobj |
absence,in |
R4088 |
T14663 |
T14664 |
nsubj |
binding,leads |
R4089 |
T14450 |
T14449 |
prep |
of,absence |
R4090 |
T14665 |
T14663 |
prep |
to,binding |
R4091 |
T14666 |
T14667 |
det |
the,oocyte |
R4092 |
T14667 |
T14665 |
pobj |
oocyte,to |
R4093 |
T14451 |
T14452 |
compound |
m,calpain |
R4094 |
T14668 |
T14664 |
prep |
to,leads |
R4095 |
T14669 |
T14670 |
amod |
increased,calcium |
R4096 |
T14670 |
T14668 |
pobj |
calcium,to |
R4097 |
T14452 |
T14450 |
pobj |
calpain,of |
R4098 |
T14671 |
T14670 |
amod |
cytoplasmic,calcium |
R4099 |
T14672 |
T14673 |
dep |
which,triggers |
R4100 |
T14673 |
T14670 |
relcl |
triggers,calcium |
R4101 |
T14453 |
T14452 |
punct |
-,calpain |
R4102 |
T14674 |
T14675 |
det |
the,reaction |
R4103 |
T14675 |
T14673 |
dobj |
reaction,triggers |
R4104 |
T14454 |
T14444 |
advmod |
still,are |
R4105 |
T14455 |
T14444 |
acomp |
unclear,are |
R4106 |
T14676 |
T14675 |
compound |
acrosome,reaction |
R4107 |
T14456 |
T14444 |
punct |
.,are |
R4108 |
T14677 |
T14678 |
punct |
[,46 |
R4109 |
T14678 |
T14664 |
parataxis |
46,leads |
R4110 |
T14679 |
T14678 |
punct |
],46 |
R4111 |
T14458 |
T14459 |
expl |
There,been |
R4112 |
T14680 |
T14664 |
punct |
.,leads |
R4113 |
T14682 |
T14683 |
preconj |
Both,μ |
R4114 |
T14683 |
T14684 |
nmod |
μ,calpain |
R4115 |
T14460 |
T14459 |
aux |
have,been |
R4116 |
T14684 |
T14689 |
nsubjpass |
calpain,detected |
R4117 |
T14685 |
T14683 |
punct |
-,μ |
R4118 |
T14686 |
T14683 |
cc |
and,μ |
R4119 |
T14687 |
T14683 |
conj |
m,μ |
R4120 |
T14461 |
T14462 |
amod |
several,reports |
R4121 |
T14688 |
T14684 |
punct |
-,calpain |
R4122 |
T14689 |
T14693 |
ccomp |
detected,implicated |
R4123 |
T14690 |
T14689 |
aux |
have,detected |
R4124 |
T14691 |
T14689 |
advmod |
recently,detected |
R4125 |
T14692 |
T14689 |
auxpass |
been,detected |
R4126 |
T14462 |
T14459 |
attr |
reports,been |
R4127 |
T14694 |
T14689 |
prep |
in,detected |
R4128 |
T14695 |
T14696 |
compound |
rodent,sperm |
R4129 |
T14696 |
T14694 |
pobj |
sperm,in |
R4130 |
T14463 |
T14462 |
prep |
of,reports |
R4131 |
T14697 |
T14698 |
punct |
[,47 |
R4132 |
T14698 |
T14696 |
parataxis |
47,sperm |
R4133 |
T14699 |
T14698 |
punct |
],47 |
R4134 |
T14464 |
T14465 |
compound |
m,calpain |
R4135 |
T14700 |
T14696 |
cc |
and,sperm |
R4136 |
T14701 |
T14696 |
conj |
oocytes,sperm |
R4137 |
T14702 |
T14703 |
punct |
[,45 |
R4138 |
T14781 |
T14782 |
amod |
defective,segregation |
R4139 |
T14703 |
T14701 |
parataxis |
45,oocytes |
R4140 |
T14704 |
T14703 |
punct |
],45 |
R4141 |
T14705 |
T14689 |
punct |
.,detected |
R4142 |
T14706 |
T14707 |
compound |
m,calpain |
R4143 |
T14782 |
T14778 |
conj |
segregation,Polyspermy |
R4144 |
T14707 |
T14693 |
nsubjpass |
calpain,implicated |
R4145 |
T14708 |
T14707 |
punct |
-,calpain |
R4146 |
T14709 |
T14693 |
auxpass |
was,implicated |
R4147 |
T14783 |
T14782 |
compound |
chromosome,segregation |
R4148 |
T14784 |
T14779 |
aux |
would,have |
R4149 |
T14710 |
T14693 |
prep |
in,implicated |
R4150 |
T14711 |
T14712 |
det |
the,reaction |
R4151 |
T14712 |
T14710 |
pobj |
reaction,in |
R4152 |
T14785 |
T14779 |
advmod |
both,have |
R4153 |
T14713 |
T14712 |
compound |
acrosome,reaction |
R4154 |
T14714 |
T14715 |
punct |
[,47 |
R4155 |
T14786 |
T14787 |
amod |
lethal,effects |
R4156 |
T14715 |
T14712 |
parataxis |
47,reaction |
R4157 |
T14716 |
T14715 |
punct |
],47 |
R4158 |
T14717 |
T14718 |
dep |
which,correlated |
R4159 |
T14718 |
T14712 |
relcl |
correlated,reaction |
R4160 |
T14787 |
T14779 |
dobj |
effects,have |
R4161 |
T14719 |
T14718 |
prep |
with,correlated |
R4162 |
T14720 |
T14721 |
det |
a,translocation |
R4163 |
T14788 |
T14787 |
prep |
on,effects |
R4164 |
T14721 |
T14719 |
pobj |
translocation,with |
R4165 |
T14722 |
T14721 |
prep |
of,translocation |
R4166 |
T14789 |
T14790 |
amod |
early,development |
R4167 |
T14723 |
T14724 |
compound |
m,calpain |
R4168 |
T14724 |
T14722 |
pobj |
calpain,of |
R4169 |
T14725 |
T14724 |
punct |
-,calpain |
R4170 |
T14790 |
T14788 |
pobj |
development,on |
R4171 |
T14726 |
T14721 |
prep |
to,translocation |
R4172 |
T14727 |
T14728 |
det |
the,membrane |
R4173 |
T14728 |
T14726 |
pobj |
membrane,to |
R4174 |
T14729 |
T14728 |
amod |
cortical,membrane |
R4175 |
T14730 |
T14728 |
prep |
in,membrane |
R4176 |
T14731 |
T14730 |
pobj |
oocytes,in |
R4177 |
T14791 |
T14790 |
amod |
embryonic,development |
R4178 |
T14732 |
T14733 |
advmod |
where,participate |
R4179 |
T14733 |
T14728 |
relcl |
participate,membrane |
R4180 |
T14734 |
T14733 |
nsubj |
it,participate |
R4181 |
T14792 |
T14779 |
punct |
.,have |
R4182 |
T14735 |
T14733 |
aux |
might,participate |
R4183 |
T14736 |
T14733 |
prep |
in,participate |
R4184 |
T14737 |
T14738 |
det |
the,release |
R4185 |
T14794 |
T14795 |
nsubjpass |
It,stressed |
R4186 |
T14738 |
T14736 |
pobj |
release,in |
R4187 |
T14739 |
T14738 |
prep |
of,release |
R4188 |
T14740 |
T14741 |
amod |
cortical,contents |
R4189 |
T14741 |
T14739 |
pobj |
contents,of |
R4190 |
T14796 |
T14795 |
aux |
should,stressed |
R4191 |
T14742 |
T14741 |
compound |
granule,contents |
R4192 |
T14743 |
T14741 |
acl |
required,contents |
R4193 |
T14744 |
T14745 |
aux |
to,prevent |
R4194 |
T14745 |
T14743 |
advcl |
prevent,required |
R4195 |
T14797 |
T14795 |
advmod |
also,stressed |
R4196 |
T14746 |
T14745 |
dobj |
polyspermy,prevent |
R4197 |
T14747 |
T14748 |
punct |
[,45 |
R4198 |
T14748 |
T14745 |
parataxis |
45,prevent |
R4199 |
T14749 |
T14748 |
punct |
],45 |
R4200 |
T14750 |
T14693 |
punct |
.,implicated |
R4201 |
T14798 |
T14795 |
auxpass |
be,stressed |
R4202 |
T14752 |
T14753 |
compound |
m,calpain |
R4203 |
T14753 |
T14755 |
nsubj |
calpain,relocalized |
R4204 |
T14754 |
T14753 |
punct |
-,calpain |
R4205 |
T14799 |
T14800 |
mark |
that,maintained |
R4206 |
T14756 |
T14755 |
advmod |
also,relocalized |
R4207 |
T14800 |
T14795 |
ccomp |
maintained,stressed |
R4208 |
T14757 |
T14755 |
prep |
to,relocalized |
R4209 |
T14758 |
T14759 |
det |
the,spindle |
R4210 |
T14759 |
T14757 |
pobj |
spindle,to |
R4211 |
T14801 |
T14802 |
nmod |
Capn4,cells |
R4212 |
T14760 |
T14759 |
nmod |
oocyte,spindle |
R4213 |
T14761 |
T14759 |
amod |
meiotic,spindle |
R4214 |
T14762 |
T14755 |
prep |
after,relocalized |
R4215 |
T14802 |
T14800 |
nsubjpass |
cells,maintained |
R4216 |
T14763 |
T14762 |
pobj |
fertilization,after |
R4217 |
T14764 |
T14755 |
punct |
", ",relocalized |
R4218 |
T14803 |
T14801 |
punct |
-,Capn4 |
R4219 |
T14765 |
T14766 |
dep |
were,involved |
R4220 |
T14766 |
T14755 |
ccomp |
involved,relocalized |
R4221 |
T14767 |
T14766 |
nsubjpass |
it,involved |
R4222 |
T14804 |
T14801 |
punct |
/,Capn4 |
R4223 |
T14768 |
T14766 |
aux |
could,involved |
R4224 |
T14769 |
T14766 |
auxpass |
be,involved |
R4225 |
T14770 |
T14766 |
prep |
in,involved |
R4226 |
T14771 |
T14772 |
compound |
chromosome,segregation |
R4227 |
T14772 |
T14770 |
pobj |
segregation,in |
R4228 |
T14773 |
T14774 |
punct |
[,45 |
R4229 |
T14805 |
T14801 |
punct |
-,Capn4 |
R4230 |
T14774 |
T14766 |
parataxis |
45,involved |
R4231 |
T14775 |
T14774 |
punct |
],45 |
R4232 |
T14776 |
T14755 |
punct |
.,relocalized |
R4233 |
T14806 |
T14802 |
nmod |
ES,cells |
R4234 |
T14778 |
T14779 |
nsubj |
Polyspermy,have |
R4235 |
T14807 |
T14806 |
cc |
and,ES |
R4236 |
T14780 |
T14778 |
cc |
or,Polyspermy |
R4237 |
T14808 |
T14806 |
conj |
MEF,ES |
R4238 |
T14809 |
T14802 |
prep |
from,cells |
R4239 |
T14810 |
T14811 |
det |
the,allele |
R4240 |
T14811 |
T14809 |
pobj |
allele,from |
R4241 |
T14812 |
T14811 |
amod |
presumptive,allele |
R4242 |
T14888 |
T14887 |
punct |
", ",exist |
R4243 |
T14813 |
T14811 |
amod |
hypomorphic,allele |
R4244 |
T14889 |
T14890 |
npadvmod |
calpain,independent |
R4245 |
T14814 |
T14800 |
aux |
can,maintained |
R4246 |
T14890 |
T14892 |
amod |
independent,mechanisms |
R4247 |
T14891 |
T14890 |
punct |
-,independent |
R4248 |
T14892 |
T14887 |
nsubj |
mechanisms,exist |
R4249 |
T14815 |
T14800 |
auxpass |
be,maintained |
R4250 |
T14893 |
T14892 |
prep |
for,mechanisms |
R4251 |
T14894 |
T14893 |
pcomp |
protecting,for |
R4252 |
T14816 |
T14800 |
prep |
in,maintained |
R4253 |
T14895 |
T14896 |
compound |
cell,viability |
R4254 |
T14896 |
T14894 |
dobj |
viability,protecting |
R4255 |
T14817 |
T14816 |
pobj |
culture,in |
R4256 |
T14897 |
T14887 |
aux |
might,exist |
R4257 |
T14898 |
T14887 |
prep |
in,exist |
R4258 |
T14899 |
T14900 |
det |
these,lines |
R4259 |
T14818 |
T14800 |
prep |
despite,maintained |
R4260 |
T14900 |
T14898 |
pobj |
lines,in |
R4261 |
T14901 |
T14900 |
compound |
cell,lines |
R4262 |
T14819 |
T14820 |
det |
an,lack |
R4263 |
T14902 |
T14903 |
dep |
that,are |
R4264 |
T14903 |
T14887 |
ccomp |
are,exist |
R4265 |
T14904 |
T14903 |
acomp |
absent,are |
R4266 |
T14820 |
T14818 |
pobj |
lack,despite |
R4267 |
T14905 |
T14903 |
prep |
in,are |
R4268 |
T14906 |
T14907 |
amod |
early,cells |
R4269 |
T14821 |
T14820 |
amod |
apparent,lack |
R4270 |
T14907 |
T14905 |
pobj |
cells,in |
R4271 |
T14908 |
T14907 |
amod |
embryonic,cells |
R4272 |
T14909 |
T14887 |
punct |
.,exist |
R4273 |
T14822 |
T14820 |
prep |
of,lack |
R4274 |
T14911 |
T14912 |
det |
The,failure |
R4275 |
T14912 |
T14914 |
nsubj |
failure,suggests |
R4276 |
T14823 |
T14824 |
compound |
calpain,activity |
R4277 |
T14913 |
T14912 |
amod |
repeated,failure |
R4278 |
T14915 |
T14916 |
aux |
to,achieve |
R4279 |
T14916 |
T14912 |
acl |
achieve,failure |
R4280 |
T14917 |
T14918 |
compound |
gene,conversion |
R4281 |
T14824 |
T14822 |
pobj |
activity,of |
R4282 |
T14918 |
T14916 |
dobj |
conversion,achieve |
R4283 |
T14919 |
T14918 |
prep |
of,conversion |
R4284 |
T14920 |
T14919 |
pobj |
Capn2,of |
R4285 |
T14825 |
T14800 |
punct |
", ",maintained |
R4286 |
T14921 |
T14920 |
punct |
+,Capn2 |
R4287 |
T14922 |
T14920 |
punct |
/,Capn2 |
R4288 |
T14923 |
T14920 |
punct |
-,Capn2 |
R4289 |
T14826 |
T14827 |
mark |
as,assessed |
R4290 |
T14924 |
T14918 |
prep |
to,conversion |
R4291 |
T14925 |
T14924 |
pobj |
Capn2,to |
R4292 |
T14827 |
T14800 |
advcl |
assessed,maintained |
R4293 |
T14926 |
T14925 |
punct |
-,Capn2 |
R4294 |
T14927 |
T14925 |
punct |
/,Capn2 |
R4295 |
T14928 |
T14925 |
punct |
-,Capn2 |
R4296 |
T14828 |
T14827 |
prep |
by,assessed |
R4297 |
T14929 |
T14930 |
compound |
ES,cells |
R4298 |
T14930 |
T14918 |
appos |
cells,conversion |
R4299 |
T14829 |
T14830 |
compound |
casein,zymography |
R4300 |
T14931 |
T14918 |
prep |
by,conversion |
R4301 |
T14932 |
T14931 |
pobj |
selection,by |
R4302 |
T14933 |
T14932 |
prep |
of,selection |
R4303 |
T14830 |
T14828 |
pobj |
zymography,by |
R4304 |
T14934 |
T14933 |
pobj |
clones,of |
R4305 |
T14935 |
T14932 |
prep |
in,selection |
R4306 |
T14831 |
T14828 |
cc |
or,by |
R4307 |
T14936 |
T14937 |
amod |
high,concentrations |
R4308 |
T14937 |
T14935 |
pobj |
concentrations,in |
R4309 |
T14938 |
T14937 |
prep |
of,concentrations |
R4310 |
T14832 |
T14828 |
conj |
by,by |
R4311 |
T14939 |
T14938 |
pobj |
G418,of |
R4312 |
T14940 |
T14941 |
mark |
that,compromises |
R4313 |
T14833 |
T14834 |
det |
the,appearance |
R4314 |
T14941 |
T14914 |
ccomp |
compromises,suggests |
R4315 |
T14942 |
T14943 |
det |
the,state |
R4316 |
T14943 |
T14941 |
nsubj |
state,compromises |
R4317 |
T14834 |
T14832 |
pobj |
appearance,by |
R4318 |
T14944 |
T14943 |
amod |
homozygous,state |
R4319 |
T14945 |
T14943 |
compound |
mutant,state |
R4320 |
T14946 |
T14941 |
advmod |
somehow,compromises |
R4321 |
T14835 |
T14834 |
prep |
of,appearance |
R4322 |
T14947 |
T14948 |
compound |
cell,viability |
R4323 |
T14948 |
T14941 |
dobj |
viability,compromises |
R4324 |
T14836 |
T14837 |
amod |
characteristic,products |
R4325 |
T14949 |
T14948 |
cc |
or,viability |
R4326 |
T14950 |
T14951 |
amod |
clonal,selection |
R4327 |
T14951 |
T14948 |
conj |
selection,viability |
R4328 |
T14837 |
T14835 |
pobj |
products,of |
R4329 |
T14952 |
T14951 |
prep |
of,selection |
R4330 |
T14953 |
T14954 |
compound |
ES,cells |
R4331 |
T14954 |
T14952 |
pobj |
cells,of |
R4332 |
T14955 |
T14914 |
punct |
.,suggests |
R4333 |
T14838 |
T14837 |
compound |
spectrin,products |
R4334 |
T14957 |
T14958 |
nsubj |
Efforts,are |
R4335 |
T14959 |
T14960 |
advmod |
under,way |
R4336 |
T14839 |
T14837 |
compound |
breakdown,products |
R4337 |
T14960 |
T14958 |
advmod |
way,are |
R4338 |
T14961 |
T14962 |
aux |
to,express |
R4339 |
T14840 |
T14795 |
punct |
.,stressed |
R4340 |
T14962 |
T14958 |
xcomp |
express,are |
R4341 |
T14963 |
T14964 |
det |
the,subunit |
R4342 |
T14842 |
T14843 |
nsubj |
It,is |
R4343 |
T14844 |
T14843 |
acomp |
conceivable,is |
R4344 |
T14964 |
T14962 |
dobj |
subunit,express |
R4345 |
T14965 |
T14964 |
nmod |
m,subunit |
R4346 |
T14966 |
T14965 |
punct |
-,m |
R4347 |
T14845 |
T14843 |
punct |
", ",is |
R4348 |
T14967 |
T14965 |
nummod |
80,m |
R4349 |
T14968 |
T14964 |
compound |
k,subunit |
R4350 |
T14969 |
T14964 |
prep |
from,subunit |
R4351 |
T14846 |
T14847 |
mark |
as,discussed |
R4352 |
T14970 |
T14971 |
det |
a,transgene |
R4353 |
T14971 |
T14969 |
pobj |
transgene,from |
R4354 |
T14847 |
T14843 |
advcl |
discussed,is |
R4355 |
T14972 |
T14971 |
compound |
Capn2,transgene |
R4356 |
T14973 |
T14971 |
compound |
cDNA,transgene |
R4357 |
T14848 |
T14847 |
advmod |
earlier,discussed |
R4358 |
T14974 |
T14971 |
compound |
rescue,transgene |
R4359 |
T14975 |
T14976 |
amod |
prior,to |
R4360 |
T14976 |
T14962 |
prep |
to,express |
R4361 |
T14849 |
T14843 |
punct |
", ",is |
R4362 |
T14977 |
T14978 |
compound |
gene,conversion |
R4363 |
T14978 |
T14976 |
pobj |
conversion,to |
R4364 |
T14979 |
T14962 |
prep |
in,express |
R4365 |
T14850 |
T14851 |
mark |
that,retained |
R4366 |
T14980 |
T14979 |
pobj |
order,in |
R4367 |
T14981 |
T14982 |
aux |
to,rescue |
R4368 |
T14851 |
T14843 |
ccomp |
retained,is |
R4369 |
T14982 |
T14980 |
acl |
rescue,order |
R4370 |
T14983 |
T14982 |
advmod |
preemptively,rescue |
R4371 |
T14984 |
T14985 |
compound |
m,calpain |
R4372 |
T14852 |
T14853 |
det |
a,amount |
R4373 |
T14985 |
T14987 |
compound |
calpain,activity |
R4374 |
T14986 |
T14985 |
punct |
-,calpain |
R4375 |
T14987 |
T14982 |
dobj |
activity,rescue |
R4376 |
T14853 |
T14851 |
nsubjpass |
amount,retained |
R4377 |
T14988 |
T14989 |
mark |
before,lost |
R4378 |
T14989 |
T14982 |
advcl |
lost,rescue |
R4379 |
T14990 |
T14991 |
det |
the,allele |
R4380 |
T14854 |
T14853 |
compound |
trace,amount |
R4381 |
T14991 |
T14989 |
nsubjpass |
allele,lost |
R4382 |
T14992 |
T14991 |
amod |
remaining,allele |
R4383 |
T14993 |
T14991 |
amod |
endogenous,allele |
R4384 |
T14855 |
T14853 |
prep |
of,amount |
R4385 |
T14856 |
T14857 |
compound |
calpain,activity |
R4386 |
T14994 |
T14995 |
amod |
wild,type |
R4387 |
T14995 |
T14991 |
compound |
type,allele |
R4388 |
T14996 |
T14995 |
punct |
-,type |
R4389 |
T14857 |
T14855 |
pobj |
activity,of |
R4390 |
T14997 |
T14989 |
auxpass |
is,lost |
R4391 |
T14998 |
T14958 |
punct |
.,are |
R4392 |
T14858 |
T14853 |
punct |
", ",amount |
R4393 |
T15000 |
T15001 |
advmod |
However,hampered |
R4394 |
T14859 |
T14853 |
prep |
beneath,amount |
R4395 |
T15002 |
T15001 |
punct |
", ",hampered |
R4396 |
T15003 |
T15001 |
nsubj |
difficulty,hampered |
R4397 |
T14860 |
T14859 |
pobj |
levels,beneath |
R4398 |
T15004 |
T15003 |
prep |
in,difficulty |
R4399 |
T15005 |
T15004 |
pcomp |
achieving,in |
R4400 |
T15006 |
T15007 |
amod |
stable,expression |
R4401 |
T14861 |
T14860 |
amod |
detectable,levels |
R4402 |
T15007 |
T15005 |
dobj |
expression,achieving |
R4403 |
T15008 |
T15007 |
prep |
of,expression |
R4404 |
T15009 |
T15010 |
det |
the,transgene |
R4405 |
T14862 |
T14861 |
prep |
by,detectable |
R4406 |
T15010 |
T15008 |
pobj |
transgene,of |
R4407 |
T15011 |
T15010 |
compound |
rescue,transgene |
R4408 |
T14863 |
T14864 |
det |
these,methods |
R4409 |
T15012 |
T15001 |
aux |
has,hampered |
R4410 |
T15013 |
T15014 |
advmod |
thus,far |
R4411 |
T15014 |
T15001 |
advmod |
far,hampered |
R4412 |
T14864 |
T14862 |
pobj |
methods,by |
R4413 |
T15015 |
T15016 |
det |
these,attempts |
R4414 |
T15016 |
T15001 |
dobj |
attempts,hampered |
R4415 |
T14865 |
T14851 |
punct |
", ",retained |
R4416 |
T15017 |
T15001 |
punct |
.,hampered |
R4417 |
T14866 |
T14851 |
auxpass |
is,retained |
R4418 |
T15019 |
T15020 |
nummod |
One,curiosity |
R4419 |
T15020 |
T15021 |
nsubj |
curiosity,was |
R4420 |
T14867 |
T14851 |
prep |
from,retained |
R4421 |
T15022 |
T15023 |
dep |
which,arose |
R4422 |
T15023 |
T15020 |
relcl |
arose,curiosity |
R4423 |
T14868 |
T14869 |
det |
this,allele |
R4424 |
T15024 |
T15023 |
prep |
during,arose |
R4425 |
T15025 |
T15026 |
det |
the,genotyping |
R4426 |
T15026 |
T15024 |
pobj |
genotyping,during |
R4427 |
T14869 |
T14867 |
pobj |
allele,from |
R4428 |
T15027 |
T15026 |
prep |
of,genotyping |
R4429 |
T15028 |
T15027 |
pobj |
progeny,of |
R4430 |
T15029 |
T15028 |
prep |
from,progeny |
R4431 |
T14870 |
T14869 |
compound |
mutant,allele |
R4432 |
T15030 |
T15031 |
amod |
heterozygous,interbreeding |
R4433 |
T15031 |
T15029 |
pobj |
interbreeding,from |
R4434 |
T15032 |
T15033 |
det |
the,ratio |
R4435 |
T15033 |
T15021 |
attr |
ratio,was |
R4436 |
T15034 |
T15035 |
advmod |
highly,non-Mendelian |
R4437 |
T14871 |
T14869 |
compound |
Capn4,allele |
R4438 |
T15035 |
T15033 |
amod |
non-Mendelian,ratio |
R4439 |
T15036 |
T15033 |
prep |
of,ratio |
R4440 |
T15037 |
T15036 |
pobj |
Capn2,of |
R4441 |
T14872 |
T14843 |
punct |
", ",is |
R4442 |
T15038 |
T15037 |
punct |
+,Capn2 |
R4443 |
T15039 |
T15037 |
punct |
/,Capn2 |
R4444 |
T15040 |
T15037 |
punct |
+,Capn2 |
R4445 |
T14873 |
T14843 |
cc |
and,is |
R4446 |
T15041 |
T15042 |
punct |
(,% |
R4447 |
T15042 |
T15037 |
parataxis |
%,Capn2 |
R4448 |
T15043 |
T15042 |
nummod |
11.6,% |
R4449 |
T14874 |
T14875 |
nsubj |
it,is |
R4450 |
T15044 |
T15042 |
punct |
),% |
R4451 |
T15045 |
T15033 |
prep |
to,ratio |
R4452 |
T14875 |
T14843 |
conj |
is,is |
R4453 |
T15046 |
T15045 |
pobj |
Capn2,to |
R4454 |
T15047 |
T15046 |
punct |
+,Capn2 |
R4455 |
T15048 |
T15046 |
punct |
/,Capn2 |
R4456 |
T14876 |
T14875 |
acomp |
sufficient,is |
R4457 |
T15049 |
T15046 |
punct |
-,Capn2 |
R4458 |
T14877 |
T14876 |
prep |
for,sufficient |
R4459 |
T15050 |
T15051 |
punct |
(,% |
R4460 |
T14878 |
T14877 |
pcomp |
maintaining,for |
R4461 |
T15051 |
T15046 |
parataxis |
%,Capn2 |
R4462 |
T15052 |
T15051 |
nummod |
88.4,% |
R4463 |
T14879 |
T14880 |
det |
the,viability |
R4464 |
T15053 |
T15051 |
punct |
),% |
R4465 |
T15054 |
T15033 |
prep |
of,ratio |
R4466 |
T15055 |
T15054 |
pobj |
weanlings,of |
R4467 |
T14880 |
T14878 |
dobj |
viability,maintaining |
R4468 |
T15056 |
T15021 |
punct |
.,was |
R4469 |
T15058 |
T15059 |
prep |
At,is |
R4470 |
T14881 |
T14880 |
prep |
of,viability |
R4471 |
T15060 |
T15058 |
amod |
present,At |
R4472 |
T14882 |
T14883 |
det |
the,cells |
R4473 |
T15061 |
T15059 |
punct |
", ",is |
R4474 |
T15062 |
T15059 |
expl |
there,is |
R4475 |
T14883 |
T14881 |
pobj |
cells,of |
R4476 |
T15063 |
T15064 |
det |
no,explanation |
R4477 |
T15064 |
T15059 |
attr |
explanation,is |
R4478 |
T15065 |
T15064 |
amod |
obvious,explanation |
R4479 |
T14884 |
T14875 |
punct |
.,is |
R4480 |
T15066 |
T15064 |
prep |
for,explanation |
R4481 |
T15067 |
T15068 |
det |
this,result |
R4482 |
T14886 |
T14887 |
advmod |
Furthermore,exist |
R4483 |
T15068 |
T15066 |
pobj |
result,for |
R4484 |
T15069 |
T15059 |
punct |
.,is |
R4485 |
T15071 |
T15072 |
nsubj |
Crosses,produced |
R4486 |
T15073 |
T15071 |
prep |
between,Crosses |
R4487 |
T15074 |
T15075 |
amod |
wild,type |
R4488 |
T15100 |
T15098 |
pobj |
line,involving |
R4489 |
T15075 |
T15077 |
nmod |
type,mice |
R4490 |
T15076 |
T15075 |
punct |
-,type |
R4491 |
T15077 |
T15073 |
pobj |
mice,between |
R4492 |
T15078 |
T15075 |
cc |
and,type |
R4493 |
T15079 |
T15075 |
conj |
heterozygous,type |
R4494 |
T15080 |
T15072 |
advmod |
also,produced |
R4495 |
T15101 |
T15100 |
nmod |
Capn4,line |
R4496 |
T15081 |
T15072 |
dobj |
progeny,produced |
R4497 |
T15082 |
T15081 |
prep |
with,progeny |
R4498 |
T15083 |
T15084 |
det |
a,proportion |
R4499 |
T15084 |
T15082 |
pobj |
proportion,with |
R4500 |
T15102 |
T15100 |
amod |
transgenic,line |
R4501 |
T15085 |
T15084 |
amod |
greater,proportion |
R4502 |
T15103 |
T15100 |
acl |
generated,line |
R4503 |
T15086 |
T15087 |
mark |
than,expected |
R4504 |
T15087 |
T15085 |
advcl |
expected,greater |
R4505 |
T15104 |
T15103 |
agent |
by,generated |
R4506 |
T15088 |
T15084 |
prep |
of,proportion |
R4507 |
T15089 |
T15090 |
amod |
heterozygous,offspring |
R4508 |
T15105 |
T15104 |
pobj |
Zimmerman,by |
R4509 |
T15090 |
T15088 |
pobj |
offspring,of |
R4510 |
T15091 |
T15072 |
punct |
.,produced |
R4511 |
T15106 |
T15105 |
cc |
and,Zimmerman |
R4512 |
T15093 |
T15094 |
advmod |
Interestingly,yielded |
R4513 |
T15107 |
T15105 |
conj |
colleagues,Zimmerman |
R4514 |
T15095 |
T15094 |
punct |
", ",yielded |
R4515 |
T15096 |
T15097 |
amod |
heterozygous,crosses |
R4516 |
T15097 |
T15094 |
nsubj |
crosses,yielded |
R4517 |
T15108 |
T15094 |
dobj |
progeny,yielded |
R4518 |
T15098 |
T15097 |
prep |
involving,crosses |
R4519 |
T15099 |
T15100 |
det |
the,line |
R4520 |
T15109 |
T15108 |
prep |
with,progeny |
R4521 |
T15110 |
T15111 |
det |
a,skewing |
R4522 |
T15111 |
T15109 |
pobj |
skewing,with |
R4523 |
T15112 |
T15111 |
amod |
similar,skewing |
R4524 |
T15113 |
T15114 |
punct |
", ",extreme |
R4525 |
T15114 |
T15111 |
parataxis |
extreme,skewing |
R4526 |
T15115 |
T15114 |
mark |
if,extreme |
R4527 |
T15116 |
T15114 |
advmod |
less,extreme |
R4528 |
T15117 |
T15114 |
punct |
", ",extreme |
R4529 |
T15118 |
T15111 |
prep |
in,skewing |
R4530 |
T15119 |
T15118 |
pobj |
favor,in |
R4531 |
T15120 |
T15119 |
prep |
of,favor |
R4532 |
T15121 |
T15122 |
det |
the,genotype |
R4533 |
T15122 |
T15120 |
pobj |
genotype,of |
R4534 |
T15123 |
T15122 |
amod |
heterozygous,genotype |
R4535 |
T15124 |
T15094 |
punct |
.,yielded |
R4536 |
T15126 |
T15127 |
prep |
Out,were |
R4537 |
T15128 |
T15126 |
prep |
of,Out |
R4538 |
T15129 |
T15130 |
det |
a,total |
R4539 |
T15130 |
T15128 |
pobj |
total,of |
R4540 |
T15131 |
T15130 |
prep |
of,total |
R4541 |
T15132 |
T15133 |
nummod |
80,animals |
R4542 |
T15133 |
T15131 |
pobj |
animals,of |
R4543 |
T15134 |
T15133 |
amod |
genotyped,animals |
R4544 |
T15135 |
T15127 |
punct |
", ",were |
R4545 |
T15136 |
T15137 |
nummod |
22.5,% |
R4546 |
T15137 |
T15127 |
nsubj |
%,were |
R4547 |
T15138 |
T15139 |
amod |
wild,type |
R4548 |
T15139 |
T15127 |
attr |
type,were |
R4549 |
T15140 |
T15139 |
punct |
-,type |
R4550 |
T15141 |
T15127 |
cc |
and,were |
R4551 |
T15142 |
T15143 |
nummod |
77.5,% |
R4552 |
T15143 |
T15144 |
nsubj |
%,were |
R4553 |
T15144 |
T15127 |
conj |
were,were |
R4554 |
T15145 |
T15144 |
acomp |
heterozygous,were |
R4555 |
T15146 |
T15127 |
punct |
", ",were |
R4556 |
T15147 |
T15148 |
mark |
with,observed |
R4557 |
T15148 |
T15127 |
advcl |
observed,were |
R4558 |
T15149 |
T15150 |
det |
no,progeny |
R4559 |
T15150 |
T15148 |
nsubj |
progeny,observed |
R4560 |
T15151 |
T15152 |
amod |
homozygous,null |
R4561 |
T15152 |
T15150 |
amod |
null,progeny |
R4562 |
T15153 |
T15154 |
punct |
[,32 |
R4563 |
T15154 |
T15148 |
parataxis |
32,observed |
R4564 |
T15155 |
T15154 |
punct |
],32 |
R4565 |
T15156 |
T15127 |
punct |
.,were |
R4566 |
T15158 |
T15159 |
det |
These,observations |
R4567 |
T15159 |
T15160 |
nsubj |
observations,suggest |
R4568 |
T15161 |
T15162 |
det |
a,advantage |
R4569 |
T15162 |
T15160 |
dobj |
advantage,suggest |
R4570 |
T15163 |
T15162 |
amod |
developmental,advantage |
R4571 |
T15164 |
T15162 |
acl |
associated,advantage |
R4572 |
T15165 |
T15164 |
prep |
with,associated |
R4573 |
T15166 |
T15167 |
amod |
reduced,expression |
R4574 |
T15167 |
T15165 |
pobj |
expression,with |
R4575 |
T15168 |
T15167 |
compound |
calpain,expression |
R4576 |
T15169 |
T15160 |
punct |
.,suggest |
R4577 |
T15171 |
T15172 |
advmod |
Perhaps,reveal |
R4578 |
T15173 |
T15174 |
amod |
future,studies |
R4579 |
T15174 |
T15172 |
nsubj |
studies,reveal |
R4580 |
T15175 |
T15172 |
aux |
will,reveal |
R4581 |
T15176 |
T15177 |
det |
a,basis |
R4582 |
T15177 |
T15172 |
dobj |
basis,reveal |
R4583 |
T15178 |
T15177 |
amod |
mechanistic,basis |
R4584 |
T15179 |
T15177 |
prep |
for,basis |
R4585 |
T15180 |
T15179 |
pobj |
this,for |
R4586 |
T15181 |
T15172 |
punct |
.,reveal |