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Id Subject Object Predicate Lexical cue
T22839 2635-2636 . denotes .
T22894 2805-2809 JJ denotes wild
T22893 2802-2804 NN denotes bp
T22892 2798-2801 CD denotes 429
T22891 2815-2821 NN denotes signal
T22890 2794-2797 DT denotes the
T22889 2791-2793 IN denotes of
T22888 2783-2790 NN denotes absence
T22887 2779-2782 DT denotes the
T22886 2776-2778 IN denotes by
T22885 2769-2775 VBN denotes marked
T22884 2767-2769 , denotes ,
T22883 2766-2767 SYM denotes -
T22882 2765-2766 HYPH denotes /
T22881 2764-2765 SYM denotes -
T22880 2759-2764 NN denotes Capn2
T22879 2753-2754 CD denotes 4
T22878 2752-2753 SYM denotes #
T22877 2745-2751 NN denotes embryo
T22876 2755-2758 VBD denotes was
T22875 2739-2744 IN denotes while
T22874 2737-2738 SYM denotes -
T22873 2736-2737 HYPH denotes /
T22872 2735-2736 SYM denotes +
T22871 2730-2735 NN denotes Capn2
T22870 2723-2724 CD denotes 6
T22869 2719-2722 CC denotes and
T22868 2717-2719 , denotes ,
T22867 2716-2717 CD denotes 5
T22866 2714-2716 , denotes ,
T22865 2713-2714 CD denotes 3
T22864 2711-2713 , denotes ,
T22863 2710-2711 CD denotes 2
T22862 2708-2710 , denotes ,
T22861 2725-2729 VBD denotes were
T22860 2706-2707 SYM denotes #
T22859 2707-2708 CD denotes 1
T22858 2698-2705 NNS denotes Embryos
T22857 2697-2822 sentence denotes Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal.
T22856 2696-2697 . denotes .
T22855 2688-2690 VBZ denotes is
T22854 2680-2687 NNS denotes embryos
T22853 2674-2675 HYPH denotes -
T22852 2675-2679 NN denotes cell
T22851 2673-2674 CD denotes 8
T22850 2670-2672 IN denotes of
T22849 2659-2669 NN denotes genotyping
T22848 2655-2658 DT denotes the
T22847 2652-2654 IN denotes of
T22846 2644-2651 NN denotes example
T22845 2641-2643 DT denotes An
T22844 2639-2640 -RRB- denotes )
T22843 2691-2696 VBN denotes shown
T22842 2638-2639 LS denotes B
T22841 2637-2638 -LRB- denotes (
T22840 2636-2697 sentence denotes (B) An example of the genotyping of 8-cell embryos is shown.
T22838 2622-2628 NN denotes mutant
T22837 2619-2621 NN denotes bp
T22836 2615-2618 CD denotes 389
T22835 2629-2635 NN denotes signal
T22834 2611-2614 DT denotes the
T22833 2608-2610 IN denotes of
T22832 2600-2607 NN denotes absence
T22831 2596-2599 DT denotes the
T22830 2593-2595 IN denotes by
T22829 2585-2592 VBN denotes denoted
T22828 2583-2585 , denotes ,
T22827 2582-2583 SYM denotes +
T22826 2581-2582 HYPH denotes /
T22825 2580-2581 SYM denotes +
T22824 2575-2580 NN denotes Capn2
T22823 2568-2569 CD denotes 7
T22822 2567-2568 SYM denotes #
T22821 2563-2566 CC denotes and
T22820 2560-2561 SYM denotes #
T22819 2561-2562 CD denotes 3
T22818 2552-2559 NNS denotes embryos
T22817 2570-2574 VBD denotes were
T22816 2544-2551 IN denotes whereas
T22815 2542-2543 SYM denotes -
T22814 2541-2542 HYPH denotes /
T22813 2540-2541 SYM denotes +
T22812 2535-2540 NN denotes Capn2
T22811 2528-2529 CD denotes 6
T22810 2524-2527 CC denotes and
T22809 2522-2524 , denotes ,
T22808 2521-2522 CD denotes 5
T22807 2519-2521 , denotes ,
T22806 2518-2519 CD denotes 4
T22805 2516-2518 , denotes ,
T22804 2515-2516 CD denotes 2
T22803 2513-2515 , denotes ,
T22802 2530-2534 VBD denotes were
T22801 2511-2512 SYM denotes #
T22800 2512-2513 CD denotes 1
T22799 2503-2510 NNS denotes Embryos
T22798 2502-2636 sentence denotes Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal.
T22797 2501-2502 . denotes .
T22796 2488-2493 NN denotes stage
T22795 2494-2501 NNS denotes embryos
T22794 2477-2487 NN denotes blastocyst
T22793 2474-2476 IN denotes of
T22792 2463-2473 NN denotes genotyping
T22791 2459-2462 DT denotes the
T22790 2456-2458 IN denotes of
T22789 2433-2447 JJ denotes representative
T22788 2431-2432 DT denotes A
T22787 2429-2430 -RRB- denotes )
T22786 2448-2455 NN denotes example
T22785 2428-2429 LS denotes A
T22784 2427-2428 -LRB- denotes (
T22783 2426-2502 sentence denotes (A) A representative example of the genotyping of blastocyst stage embryos.
T22782 2425-2426 . denotes .
T22781 2409-2417 JJ denotes internal
T22780 2418-2425 NN denotes control
T22779 2405-2408 DT denotes the
T22778 2401-2404 IN denotes for
T22777 2398-2400 NN denotes bp
T22776 2394-2397 CD denotes 213
T22775 2390-2393 CC denotes and
T22774 2388-2390 , denotes ,
T22773 2375-2381 NN denotes mutant
T22772 2382-2388 NN denotes allele
T22771 2371-2374 DT denotes the
T22770 2367-2370 IN denotes for
T22769 2364-2366 NN denotes bp
T22768 2360-2363 CD denotes 389
T22767 2358-2360 , denotes ,
T22766 2346-2347 HYPH denotes -
T22765 2347-2351 NN denotes type
T22764 2342-2346 JJ denotes wild
T22763 2352-2358 NN denotes allele
T22762 2338-2341 DT denotes the
T22761 2334-2337 IN denotes for
T22760 2331-2333 NN denotes bp
T22759 2327-2330 CD denotes 429
T22758 2322-2326 VBD denotes were
T22757 2307-2312 JJ denotes final
T22756 2313-2321 NNS denotes products
T22755 2303-2306 DT denotes The
T22754 2302-2426 sentence denotes The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control.
T22753 2301-2302 . denotes .
T22752 2294-2301 NNS denotes alleles
T22751 2289-2293 DT denotes both
T22750 2286-2288 IN denotes in
T22749 2277-2279 VBZ denotes is
T22748 2280-2285 VBN denotes found
T22747 2271-2276 WDT denotes which
T22746 2269-2271 , denotes ,
T22745 2253-2262 NN denotes targeting
T22744 2263-2269 NN denotes vector
T22743 2249-2252 DT denotes the
T22742 2246-2248 IN denotes of
T22741 2240-2241 -RRB- denotes )
T22740 2232-2240 JJ denotes upstream
T22739 2231-2232 -LRB- denotes (
T22738 2225-2230 JJ denotes short
T22737 2242-2245 NN denotes arm
T22736 2221-2224 DT denotes the
T22735 2218-2220 IN denotes in
T22734 2210-2217 VBN denotes located
T22733 2208-2210 , denotes ,
T22732 2192-2199 NN denotes control
T22731 2183-2191 JJ denotes internal
T22730 2200-2208 NN denotes sequence
T22729 2180-2182 DT denotes an
T22728 2175-2179 IN denotes with
T22727 2157-2161 VBD denotes were
T22726 2149-2156 NNS denotes alleles
T22725 2142-2148 NN denotes mutant
T22724 2139-2141 CC denotes or
T22723 2133-2134 HYPH denotes -
T22722 2129-2133 JJ denotes wild
T22721 2125-2128 DT denotes the
T22720 2134-2138 NN denotes type
T22719 2118-2124 CC denotes either
T22718 2115-2117 IN denotes in
T22717 2103-2114 RB denotes exclusively
T22716 2097-2102 VBN denotes found
T22715 2078-2087 NNS denotes reactions
T22714 2088-2096 NNS denotes segments
T22713 2069-2077 JJ denotes separate
T22712 2162-2174 VBN denotes co-amplified
T22711 2066-2068 IN denotes In
T22710 2065-2302 sentence denotes In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles.
T22709 2064-2065 . denotes .
T22708 2063-2064 NN denotes K
T22707 2052-2062 NN denotes proteinase
T22706 2047-2051 IN denotes with
T22705 2038-2046 VBN denotes digested
T22704 2033-2037 RB denotes then
T22703 2029-2032 CC denotes and
T22702 2020-2028 NNS denotes oviducts
T22701 2016-2019 DT denotes the
T22700 2011-2015 IN denotes from
T22699 1998-2002 VBD denotes were
T22698 1996-1997 -RRB- denotes )
T22697 1992-1996 NN denotes E2.5
T22696 1991-1992 -LRB- denotes (
T22695 2003-2010 VBN denotes flushed
T22694 1977-1978 HYPH denotes -
T22693 1978-1982 NN denotes cell
T22692 1976-1977 CD denotes 8
T22691 1973-1975 CC denotes or
T22690 1971-1972 -RRB- denotes )
T22689 1967-1971 NN denotes E3.5
T22688 1966-1967 -LRB- denotes (
T22687 1983-1990 NNS denotes embryos
T22686 1955-1965 NN denotes Blastocyst
T22685 1954-2065 sentence denotes Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K.
T22684 1953-1954 . denotes .
T22683 1948-1953 NNS denotes plugs
T22665 1852-1857 NN denotes Capn2
T22664 1851-1954 sentence denotes Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs.
T22663 1850-1851 . denotes .
T22662 1849-1850 CD denotes 4
T22661 1842-1848 NN denotes Figure
T22660 1839-1841 IN denotes in
T22659 1829-1838 VBN denotes described
T22658 1826-1828 IN denotes as
T22657 1813-1825 NN denotes implantation
T22656 1810-1812 IN denotes to
T22655 1804-1809 JJ denotes prior
T22654 1796-1803 NNS denotes embryos
T22653 1787-1795 VB denotes genotype
T22652 1784-1786 TO denotes to
T22651 1775-1778 VBD denotes was
T22650 1779-1783 VBN denotes used
T22649 1762-1765 NN denotes PCR
T22648 1755-1761 VBN denotes nested
T22647 1766-1774 NN denotes strategy
T22646 1753-1754 DT denotes A
T22645 1752-1851 sentence denotes A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4.
T22644 1751-1752 . denotes .
T22643 1744-1751 NNS denotes embryos
T22642 1727-1743 JJ denotes pre-implantation
T22641 1724-1726 IN denotes of
T22640 1713-1723 NN denotes Genotyping
T22907 2862-2863 . denotes .
T22906 2849-2855 NN denotes weight
T22905 2839-2848 JJ denotes molecular
T22904 2856-2862 NN denotes marker
T22903 2835-2838 DT denotes the
T22902 2825-2826 -RRB- denotes )
T22901 2827-2834 VBZ denotes denotes
T22900 2824-2825 NN denotes M
T22899 2823-2824 -LRB- denotes (
T22682 1940-1947 JJ denotes vaginal
T22681 1937-1939 IN denotes of
T22680 1926-1936 NN denotes appearance
T22679 1922-1925 DT denotes the
T22678 1919-1921 IN denotes by
T22677 1893-1906 NN denotes fertilization
T22676 1890-1892 IN denotes of
T22675 1907-1918 VBN denotes established
T22674 1885-1889 NN denotes date
T22673 1881-1884 DT denotes the
T22672 1877-1880 CC denotes and
T22671 1866-1870 VBD denotes were
T22670 1871-1876 VBN denotes mated
T22669 1859-1860 SYM denotes -
T22668 1858-1859 HYPH denotes /
T22667 1857-1858 SYM denotes +
T22666 1861-1865 NNS denotes mice
T22084 1701-1702 . denotes .
T22083 1688-1694 NN denotes mutant
T22082 1695-1701 NN denotes allele
T22081 1684-1687 DT denotes the
T22080 1681-1683 IN denotes in
T22079 1667-1668 HYPH denotes -
T22078 1668-1671 NN denotes Neo
T22077 1664-1667 NN denotes PGK
T22076 1672-1680 NN denotes cassette
T22075 1660-1663 DT denotes the
T22074 1657-1659 IN denotes by
T22073 1645-1647 VBZ denotes is
T22072 1648-1656 VBN denotes replaced
T22071 1640-1644 WDT denotes that
T22070 1627-1628 HYPH denotes -
T22069 1628-1632 NN denotes type
T22068 1623-1627 JJ denotes wild
T22067 1633-1639 NN denotes allele
T22066 1619-1622 DT denotes the
T22065 1616-1618 IN denotes of
T22064 1608-1615 NN denotes segment
T22063 1604-1607 DT denotes the
T22062 1593-1603 VBZ denotes delineates
T22061 1572-1582 JJ denotes horizontal
T22060 1570-1572 , denotes ,
T22059 1566-1570 JJ denotes grey
T22058 1583-1592 NN denotes rectangle
T22057 1564-1565 DT denotes A
T22056 1563-1702 sentence denotes A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele.
T22055 1562-1563 . denotes .
T22054 1557-1558 -RRB- denotes )
T22053 1547-1557 JJ denotes downstream
T22052 1546-1547 -LRB- denotes (
T22051 1541-1545 JJ denotes long
T22050 1559-1562 NN denotes arm
T22049 1537-1540 DT denotes the
T22048 1534-1536 IN denotes of
T22047 1528-1529 SYM denotes '
T22046 1527-1528 CD denotes 5
T22045 1530-1533 NN denotes end
T22044 1523-1526 DT denotes the
T22043 1519-1522 CC denotes and
T22042 1509-1514 JJ denotes short
T22041 1515-1518 NN denotes arm
T22040 1505-1508 DT denotes the
T22039 1502-1504 IN denotes of
T22038 1491-1501 NNS denotes boundaries
T22037 1487-1490 DT denotes the
T22036 1465-1473 JJ denotes vertical
T22035 1474-1479 NNS denotes lines
T22034 1460-1464 JJ denotes thin
T22033 1480-1486 VBP denotes denote
T22032 1454-1459 IN denotes while
T22031 1452-1454 , denotes ,
T22030 1432-1442 JJ denotes horizontal
T22029 1430-1432 , denotes ,
T22028 1426-1430 JJ denotes open
T22027 1443-1452 NN denotes rectangle
T22026 1423-1425 DT denotes an
T22025 1420-1422 IN denotes by
T22024 1406-1407 HYPH denotes -
T22023 1407-1410 NN denotes Neo
T22022 1403-1406 NN denotes PGK
T22021 1399-1402 DT denotes the
T22020 1397-1399 , denotes ,
T22019 1378-1386 JJ denotes vertical
T22018 1376-1378 , denotes ,
T22017 1387-1397 NNS denotes rectangles
T22016 1372-1376 JJ denotes open
T22015 1369-1371 IN denotes by
T22014 1411-1419 NN denotes cassette
T22013 1353-1356 VBP denotes are
T22012 1357-1368 VBN denotes represented
T22011 1347-1352 NNS denotes Exons
T22010 1346-1563 sentence denotes Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm.
T22009 1345-1346 . denotes .
T22008 1330-1335 CD denotes three
T22007 1336-1345 NNS denotes reactions
T22006 1326-1329 DT denotes the
T22005 1323-1325 IN denotes of
T22004 1318-1322 DT denotes each
T22003 1314-1317 IN denotes for
T22002 1307-1308 -RRB- denotes )
T22001 1301-1307 VBN denotes nested
T22000 1300-1301 -LRB- denotes (
T21999 1309-1313 NNS denotes sets
T21998 1296-1299 CD denotes two
T21997 1293-1295 IN denotes in
T21996 1280-1283 VBP denotes are
T21995 1278-1280 , denotes ,
T21994 1269-1278 NNS denotes triangles
T21993 1266-1268 IN denotes by
T21992 1254-1265 VBN denotes represented
T21991 1252-1254 , denotes ,
T21990 1284-1292 VBN denotes depicted
T21989 1245-1252 NNS denotes primers
T21988 1241-1244 DT denotes The
T21987 1240-1346 sentence denotes The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions.
T21986 1239-1240 . denotes .
T21985 1230-1231 HYPH denotes -
T21984 1231-1239 JJ denotes specific
T21983 1224-1230 NN denotes allele
T21982 1201-1210 NN denotes antisense
T21981 1211-1218 NNS denotes primers
T21980 1197-1200 DT denotes the
T21979 1219-1223 VBD denotes were
T21978 1189-1196 IN denotes whereas
T21977 1187-1189 , denotes ,
T21976 1178-1187 NNS denotes reactions
T21975 1173-1177 DT denotes both
T21974 1170-1172 IN denotes in
T21973 1158-1159 '' denotes '
T21972 1160-1169 NNS denotes sequences
T21971 1148-1158 JJ denotes diagnostic
T21970 1147-1148 `` denotes '
T21969 1139-1146 VB denotes amplify
T21968 1136-1138 TO denotes to
T21967 1126-1130 VBD denotes were
T21966 1131-1135 VBN denotes used
T21965 1112-1117 NN denotes sense
T21964 1107-1111 JJ denotes same
T21963 1118-1125 NNS denotes primers
T21962 1103-1106 DT denotes The
T21961 1102-1240 sentence denotes The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific.
T21960 1101-1102 . denotes .
T21959 1085-1093 JJ denotes internal
T21958 1094-1101 NN denotes control
T21957 1082-1084 DT denotes an
T21956 1079-1081 IN denotes as
T21955 1068-1069 '' denotes '
T21954 1058-1068 JJ denotes diagnostic
T21953 1057-1058 `` denotes '
T21952 1070-1078 NNS denotes products
T21951 1053-1056 DT denotes the
T21950 1048-1052 IN denotes with
T21949 1031-1034 VBD denotes was
T21948 1021-1026 JJ denotes short
T21947 1027-1030 NN denotes arm
T21946 1017-1020 DT denotes the
T21945 1010-1016 IN denotes within
T21944 1002-1009 VBN denotes located
T21943 990-992 NN denotes bp
T21942 986-989 CD denotes 213
T21941 993-1001 NN denotes sequence
T21940 984-985 DT denotes a
T21939 982-984 , denotes ,
T21938 973-982 NNS denotes reactions
T21937 968-972 DT denotes both
T21936 1035-1047 VBN denotes co-amplified
T21935 965-967 IN denotes In
T21934 964-1102 sentence denotes In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control.
T21933 963-964 . denotes .
T21932 947-956 NN denotes targeting
T21931 957-963 NN denotes vector
T21930 943-946 DT denotes the
T21929 940-942 IN denotes of
T21928 934-935 -RRB- denotes )
T21927 926-934 JJ denotes upstream
T21926 925-926 -LRB- denotes (
T21925 919-924 JJ denotes short
T21924 936-939 NN denotes arm
T21923 915-918 DT denotes the
T21922 912-914 IN denotes of
T21921 906-907 SYM denotes '
T21920 905-906 CD denotes 3
T21919 908-911 NN denotes end
T21918 901-904 DT denotes the
T21917 892-900 VBG denotes spanning
T21916 887-891 DT denotes both
T21915 885-887 , denotes ,
T21914 872-878 NN denotes mutant
T21913 879-885 NN denotes allele
T21912 868-871 DT denotes the
T21911 863-867 IN denotes from
T21910 852-854 NN denotes bp
T21909 848-851 CD denotes 389
T21908 855-862 NN denotes segment
T21907 846-847 DT denotes a
T21906 842-845 CC denotes and
T21905 829-830 HYPH denotes -
T21904 830-834 NN denotes type
T21903 825-829 JJ denotes wild
T21902 835-841 NN denotes allele
T21901 821-824 DT denotes the
T21900 816-820 IN denotes from
T21899 804-806 NN denotes bp
T21898 800-803 CD denotes 429
T21897 807-815 NN denotes fragment
T21896 798-799 DT denotes a
T21895 790-797 VB denotes amplify
T21894 787-789 TO denotes to
T21893 777-781 VBD denotes were
T21892 782-786 VBN denotes used
T21891 767-776 NNS denotes reactions
T21890 758-766 JJ denotes Separate
T21889 757-964 sentence denotes Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector.
T21888 756-757 . denotes .
T21887 749-756 NNS denotes embryos
T21886 732-748 JJ denotes pre-implantation
T21885 723-731 VB denotes genotype
T21884 720-722 TO denotes to
T21883 714-719 NN denotes order
T21882 711-713 IN denotes in
T21881 697-700 VBD denotes was
T21880 684-687 NN denotes PCR
T21879 677-683 VBN denotes nested
T21878 688-696 NN denotes strategy
T21877 675-676 DT denotes a
T21876 673-675 , denotes ,
T21875 657-664 JJ denotes genetic
T21874 665-673 NN denotes material
T21873 645-656 JJ denotes extractable
T21872 642-644 IN denotes of
T21871 633-641 NN denotes scarcity
T21870 629-632 DT denotes the
T21869 626-628 IN denotes to
T21868 701-710 VBN denotes developed
T21867 622-625 IN denotes Due
T21866 621-757 sentence denotes Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos.
T21865 620-621 . denotes .
T21864 613-620 NNS denotes embryos
T21863 596-612 JJ denotes pre-implantation
T21862 593-595 IN denotes of
T21861 582-592 NN denotes genotyping
T21860 578-581 IN denotes for
T21859 565-568 NN denotes PCR
T21858 569-577 NN denotes strategy
T21857 558-564 VBN denotes Nested
T22898 2822-2863 sentence denotes (M) denotes the molecular weight marker.
T22897 2821-2822 . denotes .
T22896 2809-2810 HYPH denotes -
T22895 2810-2814 NN denotes type
T22936 9-17 NN denotes Genotype
T22937 18-30 NN denotes distribution
T22938 31-33 IN denotes of
T22939 34-43 NN denotes offspring
T22940 44-51 VBN denotes derived
T22941 52-56 IN denotes from
T22942 57-62 NN denotes Capn2
T22943 74-78 NNS denotes mice
T22944 63-73 JJ denotes transgenic
T22945 78-79 . denotes .
T7005 0-2867 DT denotes Table 2 Genotype distribution of offspring derived from Capn2 transgenic mice. Cross (N) Age Genotype +/+ +/- -/- ND* (+/-)♀ × (+/-)♂ 199 3 weeks 23 176 0 0 (+/+)♀ × (+/-)♂ 122 3 weeks 50 72 0 0 (+/-)♀ × (+/+)♂ 103 3 weeks 28 75 0 0 (+/-)♀ × (+/-)♂ 9 E18.5 0 7 0 2 (+/-)♀ × (+/-)♂ 5 E17.5 0 5 0 0 (+/-)♀ × (+/-)♂ 9 E14.5 2 7 0 0 (+/-)♀ × (+/-)♂ 10 E11.5 3 7 0 0 (+/-)♀ × (+/-)♂ 7 E10.5 2 5 0 0 (+/-)♀ × (+/-)♂ 46 E3.5 7 39 0 0 (+/-)♀ × (+/-)♂ 48 E2.5 8 34 2 4 * ND, not determined Figure 4 Nested PCR strategy for genotyping of pre-implantation embryos. Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos. Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector. In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control. The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific. The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions. Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm. A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele. Figure 5 Genotyping of pre-implantation embryos. A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4. Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs. Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K. In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles. The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control. (A) A representative example of the genotyping of blastocyst stage embryos. Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal. (B) An example of the genotyping of 8-cell embryos is shown. Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal. (M) denotes the molecular weight marker. The
R6470 T21857 T21858 amod Nested,strategy
R6471 T21859 T21858 compound PCR,strategy
R6472 T21860 T21858 prep for,strategy
R6473 T21861 T21860 pobj genotyping,for
R6474 T21862 T21861 prep of,genotyping
R6475 T21863 T21864 amod pre-implantation,embryos
R6476 T21864 T21862 pobj embryos,of
R6477 T21865 T21858 punct .,strategy
R6478 T21867 T21868 prep Due,developed
R6479 T21869 T21867 pcomp to,Due
R6480 T21870 T21871 det the,scarcity
R6481 T21871 T21867 pobj scarcity,Due
R6482 T21872 T21871 prep of,scarcity
R6483 T21873 T21874 amod extractable,material
R6484 T21874 T21872 pobj material,of
R6485 T21875 T21874 amod genetic,material
R6486 T21876 T21868 punct ", ",developed
R6487 T21877 T21878 det a,strategy
R6488 T21878 T21868 nsubjpass strategy,developed
R6489 T21879 T21878 amod nested,strategy
R6490 T21880 T21878 compound PCR,strategy
R6491 T21881 T21868 auxpass was,developed
R6492 T21882 T21868 prep in,developed
R6493 T21883 T21882 pobj order,in
R6494 T21884 T21885 aux to,genotype
R6495 T21885 T21883 acl genotype,order
R6496 T21886 T21887 amod pre-implantation,embryos
R6497 T21887 T21885 dobj embryos,genotype
R6498 T21888 T21868 punct .,developed
R6499 T21890 T21891 amod Separate,reactions
R6500 T21891 T21892 nsubjpass reactions,used
R6501 T21893 T21892 auxpass were,used
R6502 T21894 T21895 aux to,amplify
R6503 T21895 T21892 advcl amplify,used
R6504 T21896 T21897 det a,fragment
R6505 T21897 T21895 dobj fragment,amplify
R6506 T21898 T21899 nummod 429,bp
R6507 T21899 T21897 compound bp,fragment
R6508 T21900 T21897 prep from,fragment
R6509 T21901 T21902 det the,allele
R6510 T21902 T21900 pobj allele,from
R6511 T21903 T21904 amod wild,type
R6512 T21904 T21902 compound type,allele
R6513 T21905 T21904 punct -,type
R6514 T21906 T21897 cc and,fragment
R6515 T21907 T21908 det a,segment
R6516 T21908 T21897 conj segment,fragment
R6517 T21909 T21910 nummod 389,bp
R6518 T21910 T21908 compound bp,segment
R6519 T21911 T21908 prep from,segment
R6520 T21912 T21913 det the,allele
R6521 T21913 T21911 pobj allele,from
R6522 T21914 T21913 compound mutant,allele
R6523 T21915 T21897 punct ", ",fragment
R6524 T21916 T21897 appos both,fragment
R6525 T21917 T21916 acl spanning,both
R6526 T21918 T21919 det the,end
R6527 T21919 T21917 dobj end,spanning
R6528 T21920 T21919 nummod 3,end
R6529 T21921 T21920 punct ',3
R6530 T21922 T21919 prep of,end
R6531 T21923 T21924 det the,arm
R6532 T21924 T21922 pobj arm,of
R6533 T21925 T21924 amod short,arm
R6534 T21926 T21927 punct (,upstream
R6535 T21927 T21924 parataxis upstream,arm
R6536 T21928 T21927 punct ),upstream
R6537 T21929 T21924 prep of,arm
R6538 T21930 T21931 det the,vector
R6539 T21931 T21929 pobj vector,of
R6540 T21932 T21931 compound targeting,vector
R6541 T21933 T21892 punct .,used
R6542 T21935 T21936 prep In,co-amplified
R6543 T21937 T21938 det both,reactions
R6544 T21938 T21935 pobj reactions,In
R6545 T21939 T21936 punct ", ",co-amplified
R6546 T21940 T21941 det a,sequence
R6547 T21941 T21936 nsubjpass sequence,co-amplified
R6548 T21942 T21943 nummod 213,bp
R6549 T21943 T21941 compound bp,sequence
R6550 T21944 T21941 acl located,sequence
R6551 T21945 T21944 prep within,located
R6552 T21946 T21947 det the,arm
R6553 T21947 T21945 pobj arm,within
R6554 T21948 T21947 amod short,arm
R6555 T21949 T21936 auxpass was,co-amplified
R6556 T21950 T21936 prep with,co-amplified
R6557 T21951 T21952 det the,products
R6558 T21952 T21950 pobj products,with
R6559 T21953 T21952 punct ',products
R6560 T21954 T21952 amod diagnostic,products
R6561 T21955 T21952 punct ',products
R6562 T21956 T21936 prep as,co-amplified
R6563 T21957 T21958 det an,control
R6564 T21958 T21956 pobj control,as
R6565 T21959 T21958 amod internal,control
R6566 T21960 T21936 punct .,co-amplified
R6567 T21962 T21963 det The,primers
R6568 T21963 T21966 nsubjpass primers,used
R6569 T21964 T21963 amod same,primers
R6570 T21965 T21963 compound sense,primers
R6571 T21967 T21966 auxpass were,used
R6572 T21968 T21969 aux to,amplify
R6573 T21969 T21966 advcl amplify,used
R6574 T21970 T21969 punct ',amplify
R6575 T21971 T21972 amod diagnostic,sequences
R6576 T21972 T21969 dobj sequences,amplify
R6577 T21973 T21972 punct ',sequences
R6578 T21974 T21972 prep in,sequences
R6579 T21975 T21976 det both,reactions
R6580 T21976 T21974 pobj reactions,in
R6581 T21977 T21969 punct ", ",amplify
R6582 T21978 T21979 mark whereas,were
R6583 T21979 T21969 advcl were,amplify
R6584 T21980 T21981 det the,primers
R6585 T21981 T21979 nsubj primers,were
R6586 T21982 T21981 compound antisense,primers
R6587 T21983 T21984 npadvmod allele,specific
R6588 T21984 T21979 acomp specific,were
R6589 T21985 T21984 punct -,specific
R6590 T21986 T21966 punct .,used
R6591 T21988 T21989 det The,primers
R6592 T21989 T21990 nsubjpass primers,depicted
R6593 T21991 T21989 punct ", ",primers
R6594 T21992 T21989 acl represented,primers
R6595 T21993 T21992 agent by,represented
R6596 T21994 T21993 pobj triangles,by
R6597 T21995 T21990 punct ", ",depicted
R6598 T21996 T21990 auxpass are,depicted
R6599 T21997 T21990 prep in,depicted
R6600 T21998 T21999 nummod two,sets
R6601 T21999 T21997 pobj sets,in
R6602 T22000 T21999 punct (,sets
R6603 T22001 T21999 amod nested,sets
R6604 T22002 T21999 punct ),sets
R6605 T22003 T21999 prep for,sets
R6606 T22004 T22003 pobj each,for
R6607 T22005 T22004 prep of,each
R6608 T22006 T22007 det the,reactions
R6609 T22007 T22005 pobj reactions,of
R6610 T22008 T22007 nummod three,reactions
R6611 T22009 T21990 punct .,depicted
R6612 T22011 T22012 nsubjpass Exons,represented
R6613 T22012 T22014 ccomp represented,cassette
R6614 T22013 T22012 auxpass are,represented
R6615 T22015 T22012 agent by,represented
R6616 T22016 T22017 amod open,rectangles
R6617 T22017 T22015 pobj rectangles,by
R6618 T22018 T22017 punct ", ",rectangles
R6619 T22019 T22017 amod vertical,rectangles
R6620 T22020 T22014 punct ", ",cassette
R6621 T22021 T22014 det the,cassette
R6622 T22022 T22023 compound PGK,Neo
R6623 T22023 T22014 compound Neo,cassette
R6624 T22024 T22023 punct -,Neo
R6625 T22025 T22014 agent by,cassette
R6626 T22026 T22027 det an,rectangle
R6627 T22027 T22025 pobj rectangle,by
R6628 T22028 T22027 amod open,rectangle
R6629 T22029 T22027 punct ", ",rectangle
R6630 T22030 T22027 amod horizontal,rectangle
R6631 T22031 T22014 punct ", ",cassette
R6632 T22032 T22033 mark while,denote
R6633 T22033 T22014 advcl denote,cassette
R6634 T22034 T22035 amod thin,lines
R6635 T22035 T22033 nsubj lines,denote
R6636 T22036 T22035 amod vertical,lines
R6637 T22037 T22038 det the,boundaries
R6638 T22038 T22033 dobj boundaries,denote
R6639 T22039 T22038 prep of,boundaries
R6640 T22040 T22041 det the,arm
R6641 T22041 T22039 pobj arm,of
R6642 T22042 T22041 amod short,arm
R6643 T22043 T22038 cc and,boundaries
R6644 T22044 T22045 det the,end
R6645 T22045 T22038 conj end,boundaries
R6646 T22046 T22045 nummod 5,end
R6647 T22047 T22046 punct ',5
R6648 T22048 T22045 prep of,end
R6649 T22049 T22050 det the,arm
R6650 T22050 T22048 pobj arm,of
R6651 T22051 T22050 amod long,arm
R6652 T22052 T22050 punct (,arm
R6653 T22053 T22050 amod downstream,arm
R6654 T22054 T22050 punct ),arm
R6655 T22055 T22014 punct .,cassette
R6656 T22057 T22058 det A,rectangle
R6657 T22058 T22062 nsubj rectangle,delineates
R6658 T22059 T22058 amod grey,rectangle
R6659 T22060 T22058 punct ", ",rectangle
R6660 T22061 T22058 amod horizontal,rectangle
R6661 T22063 T22064 det the,segment
R6662 T22064 T22062 dobj segment,delineates
R6663 T22065 T22064 prep of,segment
R6664 T22066 T22067 det the,allele
R6665 T22067 T22065 pobj allele,of
R6666 T22068 T22069 amod wild,type
R6667 T22069 T22067 compound type,allele
R6668 T22070 T22069 punct -,type
R6669 T22071 T22072 dep that,replaced
R6670 T22072 T22064 relcl replaced,segment
R6671 T22073 T22072 auxpass is,replaced
R6672 T22074 T22072 agent by,replaced
R6673 T22075 T22076 det the,cassette
R6674 T22076 T22074 pobj cassette,by
R6675 T22077 T22078 compound PGK,Neo
R6676 T22078 T22076 compound Neo,cassette
R6677 T22079 T22078 punct -,Neo
R6678 T22080 T22072 prep in,replaced
R6679 T22081 T22082 det the,allele
R6680 T22082 T22080 pobj allele,in
R6681 T22083 T22082 compound mutant,allele
R6682 T22084 T22062 punct .,delineates
R6683 T22641 T22640 prep of,Genotyping
R6684 T22642 T22643 amod pre-implantation,embryos
R6685 T22643 T22641 pobj embryos,of
R6686 T22644 T22640 punct .,Genotyping
R6687 T22646 T22647 det A,strategy
R6688 T22647 T22650 nsubjpass strategy,used
R6689 T22648 T22647 amod nested,strategy
R6690 T22649 T22647 compound PCR,strategy
R6691 T22651 T22650 auxpass was,used
R6692 T22652 T22653 aux to,genotype
R6693 T22653 T22650 advcl genotype,used
R6694 T22654 T22653 dobj embryos,genotype
R6695 T22655 T22656 amod prior,to
R6696 T22656 T22653 prep to,genotype
R6697 T22657 T22656 pobj implantation,to
R6698 T22658 T22659 mark as,described
R6699 T22659 T22653 advcl described,genotype
R6700 T22660 T22659 prep in,described
R6701 T22661 T22660 pobj Figure,in
R6702 T22662 T22661 nummod 4,Figure
R6703 T22663 T22650 punct .,used
R6704 T22665 T22666 nmod Capn2,mice
R6705 T22666 T22670 nsubjpass mice,mated
R6706 T22667 T22665 punct +,Capn2
R6707 T22668 T22665 punct /,Capn2
R6708 T22669 T22665 punct -,Capn2
R6709 T22671 T22670 auxpass were,mated
R6710 T22672 T22670 cc and,mated
R6711 T22673 T22674 det the,date
R6712 T22674 T22675 nsubj date,established
R6713 T22675 T22670 conj established,mated
R6714 T22676 T22674 prep of,date
R6715 T22677 T22676 pobj fertilization,of
R6716 T22678 T22675 agent by,established
R6717 T22679 T22680 det the,appearance
R6718 T22680 T22678 pobj appearance,by
R6719 T22681 T22680 prep of,appearance
R6720 T22682 T22683 amod vaginal,plugs
R6721 T22683 T22681 pobj plugs,of
R6722 T22684 T22675 punct .,established
R6723 T22686 T22687 nmod Blastocyst,embryos
R6724 T22687 T22695 nsubjpass embryos,flushed
R6725 T22688 T22689 punct (,E3.5
R6726 T22689 T22686 parataxis E3.5,Blastocyst
R6727 T22690 T22689 punct ),E3.5
R6728 T22691 T22686 cc or,Blastocyst
R6729 T22692 T22693 nummod 8,cell
R6730 T22693 T22686 conj cell,Blastocyst
R6731 T22694 T22693 punct -,cell
R6732 T22696 T22697 punct (,E2.5
R6733 T22697 T22687 parataxis E2.5,embryos
R6734 T22698 T22697 punct ),E2.5
R6735 T22699 T22695 auxpass were,flushed
R6736 T22700 T22695 prep from,flushed
R6737 T22701 T22702 det the,oviducts
R6738 T22702 T22700 pobj oviducts,from
R6739 T22703 T22695 cc and,flushed
R6740 T22704 T22705 advmod then,digested
R6741 T22705 T22695 conj digested,flushed
R6742 T22706 T22705 prep with,digested
R6743 T22707 T22708 compound proteinase,K
R6744 T22708 T22706 pobj K,with
R6745 T22709 T22695 punct .,flushed
R6746 T22711 T22712 prep In,co-amplified
R6747 T22713 T22714 amod separate,segments
R6748 T22714 T22711 pobj segments,In
R6749 T22715 T22714 compound reactions,segments
R6750 T22716 T22714 acl found,segments
R6751 T22717 T22716 advmod exclusively,found
R6752 T22718 T22716 prep in,found
R6753 T22719 T22720 preconj either,type
R6754 T22720 T22718 pobj type,in
R6755 T22721 T22720 det the,type
R6756 T22722 T22720 amod wild,type
R6757 T22723 T22720 punct -,type
R6758 T22724 T22720 cc or,type
R6759 T22725 T22720 conj mutant,type
R6760 T22726 T22712 nsubjpass alleles,co-amplified
R6761 T22727 T22712 auxpass were,co-amplified
R6762 T22728 T22712 prep with,co-amplified
R6763 T22729 T22730 det an,sequence
R6764 T22730 T22728 pobj sequence,with
R6765 T22731 T22730 amod internal,sequence
R6766 T22732 T22730 compound control,sequence
R6767 T22733 T22730 punct ", ",sequence
R6768 T22734 T22730 acl located,sequence
R6769 T22735 T22734 prep in,located
R6770 T22736 T22737 det the,arm
R6771 T22737 T22735 pobj arm,in
R6772 T22738 T22737 amod short,arm
R6773 T22739 T22737 punct (,arm
R6774 T22740 T22737 amod upstream,arm
R6775 T22741 T22737 punct ),arm
R6776 T22742 T22737 prep of,arm
R6777 T22743 T22744 det the,vector
R6778 T22744 T22742 pobj vector,of
R6779 T22745 T22744 compound targeting,vector
R6780 T22746 T22744 punct ", ",vector
R6781 T22747 T22748 dep which,found
R6782 T22748 T22744 relcl found,vector
R6783 T22749 T22748 auxpass is,found
R6784 T22750 T22748 prep in,found
R6785 T22751 T22752 det both,alleles
R6786 T22752 T22750 pobj alleles,in
R6787 T22753 T22712 punct .,co-amplified
R6788 T22755 T22756 det The,products
R6789 T22756 T22758 nsubj products,were
R6790 T22757 T22756 amod final,products
R6791 T22759 T22760 nummod 429,bp
R6792 T22760 T22758 attr bp,were
R6793 T22761 T22758 prep for,were
R6794 T22762 T22763 det the,allele
R6795 T22763 T22761 pobj allele,for
R6796 T22764 T22765 amod wild,type
R6797 T22765 T22763 compound type,allele
R6798 T22766 T22765 punct -,type
R6799 T22767 T22758 punct ", ",were
R6800 T22768 T22769 nummod 389,bp
R6801 T22769 T22758 conj bp,were
R6802 T22770 T22769 prep for,bp
R6803 T22771 T22772 det the,allele
R6804 T22772 T22770 pobj allele,for
R6805 T22773 T22772 compound mutant,allele
R6806 T22774 T22769 punct ", ",bp
R6807 T22775 T22769 cc and,bp
R6808 T22776 T22777 nummod 213,bp
R6809 T22777 T22769 conj bp,bp
R6810 T22778 T22777 prep for,bp
R6811 T22779 T22780 det the,control
R6812 T22780 T22778 pobj control,for
R6813 T22781 T22780 amod internal,control
R6814 T22782 T22758 punct .,were
R6815 T22784 T22785 punct (,A
R6816 T22785 T22786 meta A,example
R6817 T22787 T22785 punct ),A
R6818 T22788 T22786 det A,example
R6819 T22789 T22786 amod representative,example
R6820 T22790 T22786 prep of,example
R6821 T22791 T22792 det the,genotyping
R6822 T22792 T22790 pobj genotyping,of
R6823 T22793 T22792 prep of,genotyping
R6824 T22794 T22795 compound blastocyst,embryos
R6825 T22795 T22793 pobj embryos,of
R6826 T22796 T22795 compound stage,embryos
R6827 T22797 T22786 punct .,example
R6828 T22799 T22800 nmod Embryos,1
R6829 T22800 T22802 nsubj 1,were
R6830 T22801 T22800 punct #,1
R6831 T22803 T22800 punct ", ",1
R6832 T22804 T22800 conj 2,1
R6833 T22805 T22804 punct ", ",2
R6834 T22806 T22804 conj 4,2
R6835 T22807 T22806 punct ", ",4
R6836 T22808 T22806 conj 5,4
R6837 T22809 T22808 punct ", ",5
R6838 T22810 T22808 cc and,5
R6839 T22811 T22808 conj 6,5
R6840 T22812 T22802 attr Capn2,were
R6841 T22813 T22812 punct +,Capn2
R6842 T22814 T22812 punct /,Capn2
R6843 T22815 T22812 punct -,Capn2
R6844 T22816 T22817 mark whereas,were
R6845 T22817 T22802 advcl were,were
R6846 T22818 T22819 nmod embryos,3
R6847 T22819 T22817 nsubj 3,were
R6848 T22820 T22819 punct #,3
R6849 T22821 T22819 cc and,3
R6850 T22822 T22823 punct #,7
R6851 T22823 T22819 conj 7,3
R6852 T22824 T22817 attr Capn2,were
R6853 T22825 T22824 punct +,Capn2
R6854 T22826 T22824 punct /,Capn2
R6855 T22827 T22824 punct +,Capn2
R6856 T22828 T22817 punct ", ",were
R6857 T22829 T22817 advcl denoted,were
R6858 T22830 T22829 agent by,denoted
R6859 T22831 T22832 det the,absence
R6860 T22832 T22830 pobj absence,by
R6861 T22833 T22832 prep of,absence
R6862 T22834 T22835 det the,signal
R6863 T22835 T22833 pobj signal,of
R6864 T22836 T22837 nummod 389,bp
R6865 T22837 T22835 compound bp,signal
R6866 T22838 T22835 compound mutant,signal
R6867 T22839 T22802 punct .,were
R6868 T22841 T22842 punct (,B
R6869 T22842 T22843 meta B,shown
R6870 T22844 T22842 punct ),B
R6871 T22845 T22846 det An,example
R6872 T22846 T22843 nsubjpass example,shown
R6873 T22847 T22846 prep of,example
R6874 T22848 T22849 det the,genotyping
R6875 T22849 T22847 pobj genotyping,of
R6876 T22850 T22849 prep of,genotyping
R6877 T22851 T22852 nummod 8,cell
R6878 T22852 T22854 compound cell,embryos
R6879 T22853 T22852 punct -,cell
R6880 T22854 T22850 pobj embryos,of
R6881 T22855 T22843 auxpass is,shown
R6882 T22856 T22843 punct .,shown
R6883 T22858 T22859 nmod Embryos,1
R6884 T22859 T22861 nsubj 1,were
R6885 T22860 T22859 punct #,1
R6886 T22862 T22859 punct ", ",1
R6887 T22863 T22859 conj 2,1
R6888 T22864 T22863 punct ", ",2
R6889 T22865 T22863 conj 3,2
R6890 T22866 T22865 punct ", ",3
R6891 T22867 T22865 conj 5,3
R6892 T22868 T22867 punct ", ",5
R6893 T22869 T22867 cc and,5
R6894 T22870 T22867 conj 6,5
R6895 T22871 T22861 attr Capn2,were
R6896 T22872 T22871 punct +,Capn2
R6897 T22873 T22871 punct /,Capn2
R6898 T22874 T22871 punct -,Capn2
R6899 T22875 T22876 mark while,was
R6900 T22876 T22861 advcl was,were
R6901 T22877 T22876 nsubj embryo,was
R6902 T22878 T22877 punct #,embryo
R6903 T22879 T22877 nummod 4,embryo
R6904 T22880 T22876 attr Capn2,was
R6905 T22881 T22880 punct -,Capn2
R6906 T22882 T22880 punct /,Capn2
R6907 T22883 T22880 punct -,Capn2
R6908 T22884 T22880 punct ", ",Capn2
R6909 T22885 T22880 acl marked,Capn2
R6910 T22886 T22885 agent by,marked
R6911 T22887 T22888 det the,absence
R6912 T22888 T22886 pobj absence,by
R6913 T22889 T22888 prep of,absence
R6914 T22890 T22891 det the,signal
R6915 T22891 T22889 pobj signal,of
R6916 T22892 T22893 nummod 429,bp
R6917 T22893 T22891 nmod bp,signal
R6918 T22894 T22895 amod wild,type
R6919 T22895 T22891 compound type,signal
R6920 T22896 T22895 punct -,type
R6921 T22897 T22861 punct .,were
R6922 T22899 T22900 punct (,M
R6923 T22900 T22901 nsubj M,denotes
R6924 T22902 T22900 punct ),M
R6925 T22903 T22904 det the,marker
R6926 T22904 T22901 dobj marker,denotes
R6927 T22905 T22906 amod molecular,weight
R6928 T22906 T22904 compound weight,marker
R6929 T22907 T22901 punct .,denotes
R6930 T22936 T22937 compound Genotype,distribution
R6931 T22938 T22937 prep of,distribution
R6932 T22939 T22938 pobj offspring,of
R6933 T22940 T22939 acl derived,offspring
R6934 T22941 T22940 prep from,derived
R6935 T22942 T22943 nmod Capn2,mice
R6936 T22943 T22941 pobj mice,from
R6937 T22944 T22943 amod transgenic,mice
R6938 T22945 T22937 punct .,distribution

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T22582 2802-2804 SO:0000028 denotes bp
T22581 2759-2764 PR:000005015 denotes Capn2
T22580 2745-2751 UBERON:0000922 denotes embryo
T22579 2730-2735 PR:000005015 denotes Capn2
T22578 2698-2705 UBERON:0000922 denotes Embryos
T22577 2673-2687 UBERON:0019252 denotes 8-cell embryos
T22576 2619-2621 SO:0000028 denotes bp
T22575 2575-2580 PR:000005015 denotes Capn2
T22574 2552-2559 UBERON:0000922 denotes embryos
T22573 2535-2540 PR:000005015 denotes Capn2
T22572 2503-2510 UBERON:0000922 denotes Embryos
T22571 2494-2501 UBERON:0000922 denotes embryos
T22570 2477-2487 UBERON:0000358 denotes blastocyst
T22569 2398-2400 SO:0000028 denotes bp
T22568 2382-2388 SO:0001023 denotes allele
T22567 2364-2366 SO:0000028 denotes bp
T22566 2352-2358 SO:0001023 denotes allele
T22565 2331-2333 SO:0000028 denotes bp
T22564 2294-2301 SO:0001023 denotes alleles
T22563 2253-2269 SO:0001644 denotes targeting vector
T22562 2149-2156 SO:0001023 denotes alleles
T22561 2020-2028 UBERON:0000993 denotes oviducts
T22560 1976-1990 UBERON:0019252 denotes 8-cell embryos
T22559 1955-1965 UBERON:0000358 denotes Blastocyst
T22558 1940-1953 UBERON:0010148 denotes vaginal plugs
T22557 1893-1906 GO:0009566 denotes fertilization
T22556 1871-1876 GO:0007618 denotes mated
T22555 1861-1865 NCBITaxon:10088 denotes mice
T22554 1852-1857 PR:000005015 denotes Capn2
T22553 1813-1825 GO:0007566 denotes implantation
T22552 1796-1803 UBERON:0000922 denotes embryos
T22551 1744-1751 UBERON:0000922 denotes embryos
T22550 1731-1743 GO:0007566 denotes implantation
T21822 1695-1701 SO:0001023 denotes allele
T21821 1672-1680 SO:0005853 denotes cassette
T21820 1633-1639 SO:0001023 denotes allele
T21819 1411-1419 SO:0005853 denotes cassette
T21818 1347-1352 SO:0000147 denotes Exons
T21817 1245-1252 SO:0000112 denotes primers
T21816 1224-1230 SO:0001023 denotes allele
T21815 1211-1218 SO:0000112 denotes primers
T21814 1201-1210 SO:0000077 denotes antisense
T21813 1118-1125 SO:0000112 denotes primers
T21812 990-992 SO:0000028 denotes bp
T21811 947-963 SO:0001644 denotes targeting vector
T21810 879-885 SO:0001023 denotes allele
T21809 852-854 SO:0000028 denotes bp
T21808 835-841 SO:0001023 denotes allele
T21807 804-806 SO:0000028 denotes bp
T21806 749-756 UBERON:0000922 denotes embryos
T21805 736-748 GO:0007566 denotes implantation
T21804 657-664 SO:0000704 denotes genetic
T21803 613-620 UBERON:0000922 denotes embryos
T21802 600-612 GO:0007566 denotes implantation
T22930 57-62 PR:000005015 denotes Capn2
T22931 74-78 NCBITaxon:10088 denotes mice

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T22639 2856-2862 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T22638 2839-2848 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T22637 2824-2825 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes M
T22636 2805-2814 SO_EXT:wild_type_entity_or_quality denotes wild-type
T22635 2802-2804 SO_EXT:0000028 denotes bp
T22634 2766-2767 SO_EXT:sequence_nullness_or_absence denotes -
T22633 2764-2765 SO_EXT:sequence_nullness_or_absence denotes -
T22632 2759-2764 PR_EXT:000005015 denotes Capn2
T22631 2745-2751 UBERON:0000922 denotes embryo
T22630 2737-2738 SO_EXT:sequence_nullness_or_absence denotes -
T22629 2735-2736 SO_EXT:normal_or_wild_type_or_present denotes +
T22628 2730-2735 PR_EXT:000005015 denotes Capn2
T22627 2698-2705 UBERON:0000922 denotes Embryos
T22626 2675-2679 CL_GO_EXT:cell denotes cell
T22625 2673-2687 UBERON:0019252 denotes 8-cell embryos
T22624 2622-2628 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T22623 2619-2621 SO_EXT:0000028 denotes bp
T22622 2582-2583 SO_EXT:normal_or_wild_type_or_present denotes +
T22621 2580-2581 SO_EXT:normal_or_wild_type_or_present denotes +
T22620 2575-2580 PR_EXT:000005015 denotes Capn2
T22603 2232-2240 SO_EXT:sequence_upstreamness denotes upstream
T22602 2200-2208 SO_EXT:biological_sequence denotes sequence
T22601 2149-2156 SO_EXT:0001023 denotes alleles
T22600 2142-2148 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T22599 2129-2138 SO_EXT:wild_type_entity_or_quality denotes wild-type
T22598 2052-2062 GO_EXT:0008233 denotes proteinase
T22597 2020-2028 UBERON:0000993 denotes oviducts
T22596 1978-1982 CL_GO_EXT:cell denotes cell
T22595 1976-1990 UBERON:0019252 denotes 8-cell embryos
T22594 1955-1965 UBERON:0000358 denotes Blastocyst
T22593 1940-1953 UBERON:0010148 denotes vaginal plugs
T22592 1893-1906 GO:0009566 denotes fertilization
T22591 1871-1876 GO:0007618 denotes mated
T22590 1861-1865 NCBITaxon:10088 denotes mice
T22589 1859-1860 SO_EXT:sequence_nullness_or_absence denotes -
T22588 1857-1858 SO_EXT:normal_or_wild_type_or_present denotes +
T22587 1852-1857 PR_EXT:000005015 denotes Capn2
T22586 1813-1825 GO:0007566 denotes implantation
T22585 1796-1803 UBERON:0000922 denotes embryos
T22584 1744-1751 UBERON:0000922 denotes embryos
T22583 1731-1743 GO:0007566 denotes implantation
T21834 926-934 SO_EXT:sequence_upstreamness denotes upstream
T21833 879-885 SO_EXT:0001023 denotes allele
T21832 872-878 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T21831 852-854 SO_EXT:0000028 denotes bp
T21830 835-841 SO_EXT:0001023 denotes allele
T21829 825-834 SO_EXT:wild_type_entity_or_quality denotes wild-type
T21828 804-806 SO_EXT:0000028 denotes bp
T21827 749-756 UBERON:0000922 denotes embryos
T21826 736-748 GO:0007566 denotes implantation
T21825 657-664 SO_EXT:0000704 denotes genetic
T21824 613-620 UBERON:0000922 denotes embryos
T21823 600-612 GO:0007566 denotes implantation
T21856 1695-1701 SO_EXT:0001023 denotes allele
T21855 1688-1694 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T21854 1672-1680 SO_EXT:0005853 denotes cassette
T21853 1668-1671 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T21852 1664-1667 GO_EXT:phosphoglycerate_kinase denotes PGK
T21851 1648-1656 SO_EXT:sequence_substitution_process denotes replaced
T21850 1633-1639 SO_EXT:0001023 denotes allele
T21849 1623-1632 SO_EXT:wild_type_entity_or_quality denotes wild-type
T21848 1547-1557 SO_EXT:sequence_downstreamness denotes downstream
T21847 1411-1419 SO_EXT:0005853 denotes cassette
T21846 1407-1410 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T21845 1403-1406 GO_EXT:phosphoglycerate_kinase denotes PGK
T21844 1347-1352 SO_EXT:0000147 denotes Exons
T21843 1245-1252 SO_EXT:0000112 denotes primers
T21842 1224-1230 SO_EXT:0001023 denotes allele
T21841 1211-1218 SO_EXT:0000112 denotes primers
T21840 1201-1210 SO:0000077 denotes antisense
T21839 1160-1169 SO_EXT:biological_sequence denotes sequences
T21838 1118-1125 SO_EXT:0000112 denotes primers
T21837 993-1001 SO_EXT:biological_sequence denotes sequence
T21836 990-992 SO_EXT:0000028 denotes bp
T21835 947-963 SO_EXT:0001644 denotes targeting vector
T22619 2552-2559 UBERON:0000922 denotes embryos
T22618 2542-2543 SO_EXT:sequence_nullness_or_absence denotes -
T22617 2540-2541 SO_EXT:normal_or_wild_type_or_present denotes +
T22616 2535-2540 PR_EXT:000005015 denotes Capn2
T22615 2503-2510 UBERON:0000922 denotes Embryos
T22614 2494-2501 UBERON:0000922 denotes embryos
T22613 2477-2487 UBERON:0000358 denotes blastocyst
T22612 2398-2400 SO_EXT:0000028 denotes bp
T22611 2382-2388 SO_EXT:0001023 denotes allele
T22610 2375-2381 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T22609 2364-2366 SO_EXT:0000028 denotes bp
T22608 2352-2358 SO_EXT:0001023 denotes allele
T22607 2342-2351 SO_EXT:wild_type_entity_or_quality denotes wild-type
T22606 2331-2333 SO_EXT:0000028 denotes bp
T22605 2294-2301 SO_EXT:0001023 denotes alleles
T22604 2253-2269 SO_EXT:0001644 denotes targeting vector
T22932 9-17 SO_EXT:genotype_or_entity_with_genotype denotes Genotype
T22933 57-62 PR_EXT:000005015 denotes Capn2
T22934 63-73 SO_EXT:transgenic_entity denotes transgenic
T22935 74-78 NCBITaxon:10088 denotes mice