PMC:1382200 / 0-2867
Annnotations
craft-ca-core-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T533 | 29-41 | GO:0007566 | denotes | implantation |
| T534 | 42-51 | UBERON:0000922 | denotes | embryonic |
| T535 | 42-63 | GO:0009790 | denotes | embryonic development |
| T536 | 67-71 | NCBITaxon:10088 | denotes | mice |
| T537 | 134-143 | GO:0010467 | denotes | expressed |
| T538 | 168-186 | GO:0016477 | denotes | cellular migration |
| T539 | 188-198 | GO:0007049 | denotes | cell cycle |
| T540 | 239-249 | GO:0008219 | denotes | cell death |
| T541 | 335-340 | PR:000005009 | denotes | Capn1 |
| T542 | 356-361 | PR:000005015 | denotes | Capn2 |
| T543 | 388-398 | GO:0065007 | denotes | regulatory |
| T544 | 418-423 | PR:000005022 | denotes | Capn4 |
| T545 | 443-448 | NCBITaxon:10088 | denotes | mouse |
| T546 | 449-454 | PR:000005022 | denotes | Capn4 |
| T547 | 455-459 | SO:0000704 | denotes | gene |
| T548 | 525-534 | UBERON:0000922 | denotes | embryonic |
| T549 | 621-630 | NCBITaxon:40674 | denotes | mammalian |
| T550 | 631-644 | GO:0009790 | denotes | embryogenesis |
| T551 | 664-669 | PR:000005009 | denotes | Capn1 |
| T552 | 670-674 | SO:0000704 | denotes | gene |
| T553 | 700-704 | NCBITaxon:10088 | denotes | mice |
| T554 | 830-835 | GO:0016265 | denotes | death |
| T555 | 839-844 | PR:000005022 | denotes | Capn4 |
| T556 | 848-852 | NCBITaxon:10088 | denotes | mice |
| T557 | 965-970 | NCBITaxon:10088 | denotes | mouse |
| T558 | 971-976 | PR:000005015 | denotes | Capn2 |
| T559 | 977-981 | SO:0000704 | denotes | gene |
| T560 | 985-994 | UBERON:0000922 | denotes | embryonic |
| T561 | 985-1006 | CL:0002322 | denotes | embryonic stems cells |
| T562 | 1035-1041 | SO:0001023 | denotes | allele |
| T563 | 1054-1059 | NCBITaxon:10088 | denotes | mouse |
| T564 | 1095-1102 | NCBITaxon:33208 | denotes | animals |
| T565 | 1157-1166 | GO:0007566 | denotes | implanted |
| T566 | 1167-1174 | UBERON:0000922 | denotes | embryos |
| T567 | 1244-1256 | GO:0007566 | denotes | implantation |
| T568 | 1264-1271 | UBERON:0000922 | denotes | embryos |
| T569 | 1293-1299 | UBERON:0000085 | denotes | morula |
| T570 | 1315-1325 | UBERON:0000358 | denotes | blastocyts |
| T571 | 1391-1396 | PR:000005015 | denotes | Capn2 |
| T572 | 1397-1401 | SO:0000704 | denotes | gene |
| T573 | 1417-1429 | GO:0007566 | denotes | implantation |
| T574 | 1430-1439 | UBERON:0000922 | denotes | embryonic |
| T575 | 1462-1468 | UBERON:0000085 | denotes | morula |
| T576 | 1473-1483 | UBERON:0000358 | denotes | blastocyst |
| T577 | 1594-1608 | _FRAGMENT | denotes | development of |
| T578 | 1636-1642 | GO:0009790 | denotes | embryo |
| T579 | 1616-1628 | GO:0007566 | denotes | implantation |
| T580 | 1629-1635 | NCBITaxon:39107 | denotes | murine |
| T581 | 1636-1642 | UBERON:0000922 | denotes | embryo |
| T2933 | 1797-1801 | SO:0000704 | denotes | gene |
| T2934 | 1823-1828 | SO:0000704 | denotes | genes |
| T2935 | 1832-1839 | NCBITaxon:40674 | denotes | mammals |
| T2936 | 1943-1948 | PR:000005009 | denotes | Capn1 |
| T2937 | 1950-1956 | PR:000005009 | denotes | μ-80 k |
| T2938 | 1962-1967 | PR:000005015 | denotes | Capn2 |
| T2939 | 1969-1975 | PR:000005015 | denotes | m-80 k |
| T2940 | 1977-1982 | SO:0000704 | denotes | genes |
| T2941 | 2034-2044 | GO:0065007 | denotes | regulatory |
| T2942 | 2068-2073 | PR:000005022 | denotes | Capn4 |
| T2943 | 2074-2078 | SO:0000704 | denotes | gene |
| T2944 | 2084-2090 | _FRAGMENT | denotes | μ-80 k |
| T2945 | 2102-2110 | PR:000005009 | denotes | subunits |
| T2946 | 2095-2110 | PR:000005015 | denotes | m-80 k subunits |
| T2947 | 2295-2305 | GO:0010467 | denotes | expression |
| T2948 | 2338-2347 | NCBITaxon:40674 | denotes | mammalian |
| T2949 | 2348-2355 | UBERON:0000479 | denotes | tissues |
| T2950 | 2379-2386 | SO:0001060 | denotes | isoform |
| T2951 | 2471-2475 | CHEBI:29108 | denotes | Ca2+ |
| T2952 | 2527-2531 | CHEBI:29108 | denotes | Ca2+ |
| T2953 | 2562-2566 | CHEBI:29108 | denotes | Ca2+ |
| T2954 | 2767-2778 | GO:0005737 | denotes | cytoplasmic |
| T2955 | 2784-2788 | CHEBI:29108 | denotes | Ca2+ |
| T2931 | 1675-1679 | CHEBI:29108 | denotes | Ca2+ |
| T2932 | 1756-1764 | PR:000005015 | denotes | capain-2 |
| R271 | T2945 | T2944 | _lexicallyChainedTo | subunits,μ-80 k |
| R1 | T578 | T577 | _lexicallyChainedTo | embryo,development of |
craft-ca-core-ex-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T582 | 0-9 | GO_PR_EXT:calpain_2_complex | denotes | m-Calpain |
| T583 | 29-41 | GO:0007566 | denotes | implantation |
| T584 | 42-51 | UBERON:0000922 | denotes | embryonic |
| T585 | 42-63 | GO:0009790 | denotes | embryonic development |
| T586 | 67-71 | NCBITaxon:10088 | denotes | mice |
| T587 | 93-102 | GO_PR_EXT:calpain_1_complex | denotes | μ-calpain |
| T588 | 107-116 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T589 | 134-143 | GO:0010467 | denotes | expressed |
| T590 | 144-153 | GO_EXT:0008233 | denotes | proteases |
| T591 | 168-176 | CL_GO_EXT:cell | denotes | cellular |
| T592 | 168-186 | GO:0016477 | denotes | cellular migration |
| T593 | 188-192 | CL_GO_EXT:cell | denotes | cell |
| T594 | 188-198 | GO:0007049 | denotes | cell cycle |
| T595 | 239-243 | CL_GO_EXT:cell | denotes | cell |
| T596 | 239-249 | GO:0008219 | denotes | cell death |
| T597 | 263-270 | GO_EXT:dimer_macromolecular_complex | denotes | dimeric |
| T598 | 271-278 | CHEBI_GO_SO_EXT:enzyme | denotes | enzymes |
| T599 | 304-313 | GO_MOP_EXT:catalysis | denotes | catalytic |
| T600 | 324-331 | SO_EXT:sequence_coding_function | denotes | encoded |
| T601 | 335-340 | PR_EXT:000005009 | denotes | Capn1 |
| T602 | 342-351 | GO_PR_EXT:calpain_1_complex | denotes | μ-calpain |
| T603 | 356-361 | PR_EXT:000005015 | denotes | Capn2 |
| T604 | 363-372 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T605 | 388-398 | GO:0065007 | denotes | regulatory |
| T606 | 407-414 | SO_EXT:sequence_coding_function | denotes | encoded |
| T607 | 418-423 | PR_EXT:000005022 | denotes | Capn4 |
| T608 | 443-448 | NCBITaxon:10088 | denotes | mouse |
| T609 | 449-454 | PR_EXT:000005022 | denotes | Capn4 |
| T610 | 455-459 | SO_EXT:0000704 | denotes | gene |
| T611 | 475-484 | GO_PR_EXT:calpain_1_complex | denotes | μ-calpain |
| T612 | 489-498 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T613 | 525-534 | UBERON:0000922 | denotes | embryonic |
| T614 | 535-544 | GO_EXT:fatality_or_lethality | denotes | lethality |
| T615 | 606-613 | CHEBI_GO_SO_EXT:enzyme | denotes | enzymes |
| T616 | 621-630 | NCBITaxon:40674 | denotes | mammalian |
| T617 | 631-644 | GO:0009790 | denotes | embryogenesis |
| T618 | 664-669 | PR_EXT:000005009 | denotes | Capn1 |
| T619 | 670-674 | SO_EXT:0000704 | denotes | gene |
| T620 | 700-704 | NCBITaxon:10088 | denotes | mice |
| T621 | 726-735 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T622 | 769-778 | GO_PR_EXT:calpain_1_complex | denotes | μ-calpain |
| T623 | 800-809 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T624 | 830-835 | GO:0016265 | denotes | death |
| T625 | 839-844 | PR_EXT:000005022 | denotes | Capn4 |
| T626 | 844-845 | SO_EXT:sequence_nullness_or_absence | denotes | - |
| T627 | 846-847 | SO_EXT:sequence_nullness_or_absence | denotes | - |
| T628 | 848-852 | NCBITaxon:10088 | denotes | mice |
| T629 | 923-930 | SO_EXT:sequence_deletion_process | denotes | deleted |
| T630 | 944-957 | SO_EXT:coding_sequence | denotes | coding region |
| T631 | 965-970 | NCBITaxon:10088 | denotes | mouse |
| T632 | 971-976 | PR_EXT:000005015 | denotes | Capn2 |
| T633 | 977-981 | SO_EXT:0000704 | denotes | gene |
| T634 | 985-994 | UBERON:0000922 | denotes | embryonic |
| T635 | 985-1006 | CL:0002322 | denotes | embryonic stems cells |
| T636 | 1001-1006 | CL_GO_EXT:cell | denotes | cells |
| T637 | 1028-1034 | SO_EXT:sequence_altered_entity_or_alteration_process | denotes | mutant |
| T638 | 1035-1041 | SO_EXT:0001023 | denotes | allele |
| T639 | 1054-1059 | NCBITaxon:10088 | denotes | mouse |
| T640 | 1070-1078 | GO_EXT:breeding | denotes | Breeding |
| T641 | 1095-1102 | NCBITaxon:33208 | denotes | animals |
| T642 | 1132-1138 | SO_EXT:sequence_altered_entity_or_alteration_process | denotes | mutant |
| T643 | 1157-1166 | GO:0007566 | denotes | implanted |
| T644 | 1167-1174 | UBERON:0000922 | denotes | embryos |
| T645 | 1244-1256 | GO:0007566 | denotes | implantation |
| T646 | 1257-1263 | SO_EXT:sequence_altered_entity_or_alteration_process | denotes | mutant |
| T647 | 1264-1271 | UBERON:0000922 | denotes | embryos |
| T648 | 1293-1299 | UBERON:0000085 | denotes | morula |
| T649 | 1315-1325 | UBERON:0000358 | denotes | blastocyts |
| T650 | 1391-1396 | PR_EXT:000005015 | denotes | Capn2 |
| T651 | 1397-1401 | SO_EXT:0000704 | denotes | gene |
| T652 | 1417-1429 | GO:0007566 | denotes | implantation |
| T653 | 1430-1439 | UBERON:0000922 | denotes | embryonic |
| T654 | 1440-1449 | GO_EXT:fatality_or_lethality | denotes | lethality |
| T655 | 1462-1468 | UBERON:0000085 | denotes | morula |
| T656 | 1473-1483 | UBERON:0000358 | denotes | blastocyst |
| T657 | 1513-1522 | GO_PR_EXT:calpain_1_complex | denotes | μ-calpain |
| T658 | 1527-1536 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T659 | 1571-1580 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T660 | 1594-1608 | _FRAGMENT | denotes | development of |
| T661 | 1636-1642 | GO:0009790 | denotes | embryo |
| T662 | 1616-1628 | GO:0007566 | denotes | implantation |
| T663 | 1629-1635 | NCBITaxon:39107 | denotes | murine |
| T664 | 1636-1642 | UBERON:0000922 | denotes | embryo |
| T3038 | 1691-1699 | CHEBI_SO_EXT:cysteine | denotes | cysteine |
| T3039 | 1691-1709 | GO_EXT:0008234 | denotes | cysteine proteases |
| T3040 | 1719-1728 | GO_PR_EXT:calpain_1_complex | denotes | μ-calpain |
| T3041 | 1730-1739 | GO_PR_EXT:calpain_1_complex | denotes | calpain-1 |
| T3042 | 1745-1754 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T3043 | 1756-1764 | PR_EXT:000005015 | denotes | capain-2 |
| T3044 | 1756-1764 | GO_PR_EXT:calpain_2_complex | denotes | capain-2 |
| T3045 | 1797-1801 | SO_EXT:0000704 | denotes | gene |
| T3046 | 1823-1828 | SO_EXT:0000704 | denotes | genes |
| T3047 | 1832-1839 | NCBITaxon:40674 | denotes | mammals |
| T3048 | 1862-1869 | GO_EXT:dimer_macromolecular_complex | denotes | dimeric |
| T3049 | 1870-1877 | CHEBI_GO_SO_EXT:enzyme | denotes | enzymes |
| T3050 | 1908-1917 | GO_MOP_EXT:catalysis | denotes | catalytic |
| T3051 | 1928-1935 | SO_EXT:sequence_coding_function | denotes | encoded |
| T3052 | 1943-1948 | PR_EXT:000005009 | denotes | Capn1 |
| T3053 | 1950-1956 | PR_EXT:000005009 | denotes | μ-80 k |
| T3054 | 1962-1967 | PR_EXT:000005015 | denotes | Capn2 |
| T3055 | 1969-1975 | PR_EXT:000005015 | denotes | m-80 k |
| T3056 | 1977-1982 | SO_EXT:0000704 | denotes | genes |
| T3057 | 2034-2044 | GO:0065007 | denotes | regulatory |
| T3058 | 2053-2060 | SO_EXT:sequence_coding_function | denotes | encoded |
| T3059 | 2068-2073 | PR_EXT:000005022 | denotes | Capn4 |
| T3060 | 2074-2078 | SO_EXT:0000704 | denotes | gene |
| T3061 | 2084-2090 | _FRAGMENT | denotes | μ-80 k |
| T3062 | 2102-2110 | PR_EXT:000005009 | denotes | subunits |
| T3063 | 2095-2110 | PR_EXT:000005015 | denotes | m-80 k subunits |
| T3064 | 2121-2131 | CHEBI_SO_EXT:amino_acid | denotes | amino acid |
| T3065 | 2132-2140 | SO_EXT:biological_sequence | denotes | sequence |
| T3066 | 2195-2202 | CHEBI_PR_EXT:protein | denotes | protein |
| T3067 | 2295-2305 | GO:0010467 | denotes | expression |
| T3068 | 2318-2320 | _FRAGMENT | denotes | μ- |
| T3069 | 2327-2334 | GO_PR_EXT:calpain_1_complex | denotes | calpain |
| T3070 | 2325-2334 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T3071 | 2338-2347 | NCBITaxon:40674 | denotes | mammalian |
| T3072 | 2348-2355 | UBERON:0000479 | denotes | tissues |
| T3073 | 2379-2386 | SO_EXT:0001060 | denotes | isoform |
| T3074 | 2471-2475 | CHEBI:29108 | denotes | Ca2+ |
| T3075 | 2527-2531 | CHEBI:29108 | denotes | Ca2+ |
| T3076 | 2536-2545 | GO_PR_EXT:calpain_1_complex | denotes | μ-calpain |
| T3077 | 2562-2566 | CHEBI:29108 | denotes | Ca2+ |
| T3078 | 2571-2580 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T3079 | 2611-2613 | _FRAGMENT | denotes | μ- |
| T3080 | 2620-2627 | GO_PR_EXT:calpain_1_complex | denotes | calpain |
| T3081 | 2618-2627 | GO_PR_EXT:calpain_2_complex | denotes | m-calpain |
| T3082 | 2673-2680 | CHEBI_EXT:calcium | denotes | calcium |
| T3083 | 2767-2778 | GO:0005737 | denotes | cytoplasmic |
| T3084 | 2784-2788 | CHEBI:29108 | denotes | Ca2+ |
| T3037 | 1675-1679 | CHEBI:29108 | denotes | Ca2+ |
| R274 | T3062 | T3061 | _lexicallyChainedTo | subunits,μ-80 k |
| R275 | T3069 | T3068 | _lexicallyChainedTo | calpain,μ- |
| R278 | T3080 | T3079 | _lexicallyChainedTo | calpain,μ- |
| R2 | T661 | T660 | _lexicallyChainedTo | embryo,development of |
craft-sa-dev
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T665 | 0-1 | NN | denotes | m |
| T666 | 2-9 | NN | denotes | Calpain |
| T667 | 0-71 | sentence | denotes | m-Calpain is required for preimplantation embryonic development in mice |
| T668 | 1-2 | HYPH | denotes | - |
| T669 | 13-21 | VBN | denotes | required |
| T670 | 10-12 | VBZ | denotes | is |
| T671 | 22-25 | IN | denotes | for |
| T672 | 26-41 | NN | denotes | preimplantation |
| T673 | 52-63 | NN | denotes | development |
| T674 | 42-51 | JJ | denotes | embryonic |
| T675 | 64-66 | IN | denotes | in |
| T677 | 71-72 | sentence | denotes | |
| T678 | 82-92 | sentence | denotes | Background |
| T679 | 82-92 | NN | denotes | Background |
| T680 | 92-250 | sentence | denotes | μ-calpain and m-calpain are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. |
| T681 | 93-94 | NN | denotes | μ |
| T682 | 95-102 | NN | denotes | calpain |
| T683 | 94-95 | HYPH | denotes | - |
| T684 | 117-120 | VBP | denotes | are |
| T685 | 103-106 | CC | denotes | and |
| T686 | 107-108 | NN | denotes | m |
| T687 | 109-116 | NN | denotes | calpain |
| T688 | 108-109 | HYPH | denotes | - |
| T689 | 121-133 | RB | denotes | ubiquitously |
| T690 | 134-143 | VBN | denotes | expressed |
| T691 | 144-153 | NNS | denotes | proteases |
| T692 | 154-164 | VBN | denotes | implicated |
| T693 | 165-167 | IN | denotes | in |
| T694 | 168-176 | JJ | denotes | cellular |
| T695 | 177-186 | NN | denotes | migration |
| T696 | 186-188 | , | denotes | , |
| T697 | 188-192 | NN | denotes | cell |
| T698 | 193-198 | NN | denotes | cycle |
| T699 | 199-210 | NN | denotes | progression |
| T700 | 210-212 | , | denotes | , |
| T701 | 212-224 | JJ | denotes | degenerative |
| T702 | 225-234 | NNS | denotes | processes |
| T703 | 235-238 | CC | denotes | and |
| T704 | 239-243 | NN | denotes | cell |
| T705 | 244-249 | NN | denotes | death |
| T706 | 249-250 | . | denotes | . |
| T707 | 250-424 | sentence | denotes | These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (μ-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. |
| T708 | 251-256 | DT | denotes | These |
| T709 | 271-278 | NNS | denotes | enzymes |
| T710 | 257-270 | JJ | denotes | heterodimeric |
| T711 | 283-291 | VBN | denotes | composed |
| T712 | 279-282 | VBP | denotes | are |
| T713 | 292-294 | IN | denotes | of |
| T714 | 295-303 | JJ | denotes | distinct |
| T715 | 314-322 | NNS | denotes | subunits |
| T716 | 304-313 | JJ | denotes | catalytic |
| T717 | 322-324 | , | denotes | , |
| T718 | 324-331 | VBN | denotes | encoded |
| T719 | 332-334 | IN | denotes | by |
| T720 | 335-340 | NN | denotes | Capn1 |
| T721 | 341-342 | -LRB- | denotes | ( |
| T722 | 342-343 | NN | denotes | μ |
| T723 | 344-351 | NN | denotes | calpain |
| T724 | 343-344 | HYPH | denotes | - |
| T725 | 351-352 | -RRB- | denotes | ) |
| T726 | 353-355 | CC | denotes | or |
| T727 | 356-361 | NN | denotes | Capn2 |
| T728 | 362-363 | -LRB- | denotes | ( |
| T729 | 363-364 | NN | denotes | m |
| T730 | 365-372 | NN | denotes | calpain |
| T731 | 364-365 | HYPH | denotes | - |
| T732 | 372-373 | -RRB- | denotes | ) |
| T733 | 373-375 | , | denotes | , |
| T734 | 375-378 | CC | denotes | and |
| T735 | 379-380 | DT | denotes | a |
| T736 | 399-406 | NN | denotes | subunit |
| T737 | 381-387 | JJ | denotes | common |
| T738 | 388-398 | JJ | denotes | regulatory |
| T739 | 407-414 | VBN | denotes | encoded |
| T740 | 415-417 | IN | denotes | by |
| T741 | 418-423 | NN | denotes | Capn4 |
| T742 | 423-424 | . | denotes | . |
| T743 | 424-645 | sentence | denotes | Disruption of the mouse Capn4 gene abolished both μ-calpain and m-calpain activity, and resulted in embryonic lethality, thereby suggesting essential roles for one or both of these enzymes during mammalian embryogenesis. |
| T744 | 425-435 | NN | denotes | Disruption |
| T745 | 460-469 | VBD | denotes | abolished |
| T746 | 436-438 | IN | denotes | of |
| T747 | 439-442 | DT | denotes | the |
| T748 | 455-459 | NN | denotes | gene |
| T749 | 443-448 | NN | denotes | mouse |
| T750 | 449-454 | NN | denotes | Capn4 |
| T751 | 470-474 | CC | denotes | both |
| T752 | 477-484 | NN | denotes | calpain |
| T753 | 475-476 | NN | denotes | μ |
| T754 | 476-477 | HYPH | denotes | - |
| T755 | 499-507 | NN | denotes | activity |
| T756 | 485-488 | CC | denotes | and |
| T757 | 489-490 | NN | denotes | m |
| T758 | 491-498 | NN | denotes | calpain |
| T759 | 490-491 | HYPH | denotes | - |
| T760 | 507-509 | , | denotes | , |
| T761 | 509-512 | CC | denotes | and |
| T762 | 513-521 | VBD | denotes | resulted |
| T763 | 522-524 | IN | denotes | in |
| T764 | 525-534 | JJ | denotes | embryonic |
| T765 | 535-544 | NN | denotes | lethality |
| T766 | 544-546 | , | denotes | , |
| T767 | 546-553 | RB | denotes | thereby |
| T768 | 554-564 | VBG | denotes | suggesting |
| T769 | 565-574 | JJ | denotes | essential |
| T770 | 575-580 | NNS | denotes | roles |
| T771 | 581-584 | IN | denotes | for |
| T772 | 585-588 | CD | denotes | one |
| T773 | 589-591 | CC | denotes | or |
| T774 | 592-596 | DT | denotes | both |
| T775 | 597-599 | IN | denotes | of |
| T776 | 600-605 | DT | denotes | these |
| T777 | 606-613 | NNS | denotes | enzymes |
| T778 | 614-620 | IN | denotes | during |
| T779 | 621-630 | JJ | denotes | mammalian |
| T780 | 631-644 | NN | denotes | embryogenesis |
| T781 | 644-645 | . | denotes | . |
| T782 | 645-853 | sentence | denotes | Disruption of the Capn1 gene produced viable, fertile mice implying that either m-calpain could compensate for the loss of μ-calpain, or that the loss of m-calpain was responsible for death of Capn4-/- mice. |
| T783 | 646-656 | NN | denotes | Disruption |
| T784 | 675-683 | VBD | denotes | produced |
| T785 | 657-659 | IN | denotes | of |
| T786 | 660-663 | DT | denotes | the |
| T787 | 670-674 | NN | denotes | gene |
| T788 | 664-669 | NN | denotes | Capn1 |
| T789 | 684-690 | JJ | denotes | viable |
| T790 | 700-704 | NNS | denotes | mice |
| T791 | 690-692 | , | denotes | , |
| T792 | 692-699 | JJ | denotes | fertile |
| T793 | 705-713 | VBG | denotes | implying |
| T794 | 714-718 | IN | denotes | that |
| T795 | 742-752 | VB | denotes | compensate |
| T796 | 719-725 | DT | denotes | either |
| T797 | 726-727 | NN | denotes | m |
| T798 | 728-735 | NN | denotes | calpain |
| T799 | 727-728 | HYPH | denotes | - |
| T800 | 736-741 | MD | denotes | could |
| T801 | 753-756 | IN | denotes | for |
| T802 | 757-760 | DT | denotes | the |
| T803 | 761-765 | NN | denotes | loss |
| T804 | 766-768 | IN | denotes | of |
| T805 | 769-770 | NN | denotes | μ |
| T806 | 771-778 | NN | denotes | calpain |
| T807 | 770-771 | HYPH | denotes | - |
| T808 | 778-780 | , | denotes | , |
| T809 | 780-782 | CC | denotes | or |
| T810 | 783-787 | IN | denotes | that |
| T811 | 810-813 | VBD | denotes | was |
| T812 | 788-791 | DT | denotes | the |
| T813 | 792-796 | NN | denotes | loss |
| T814 | 797-799 | IN | denotes | of |
| T815 | 800-801 | NN | denotes | m |
| T816 | 802-809 | NN | denotes | calpain |
| T817 | 801-802 | HYPH | denotes | - |
| T818 | 814-825 | JJ | denotes | responsible |
| T819 | 826-829 | IN | denotes | for |
| T820 | 830-835 | NN | denotes | death |
| T821 | 836-838 | IN | denotes | of |
| T822 | 839-844 | NN | denotes | Capn4 |
| T823 | 848-852 | NNS | denotes | mice |
| T824 | 844-845 | SYM | denotes | - |
| T825 | 845-846 | HYPH | denotes | / |
| T826 | 846-847 | SYM | denotes | - |
| T827 | 852-853 | . | denotes | . |
| T828 | 853-862 | sentence | denotes | Results |
| T829 | 855-862 | NNS | denotes | Results |
| T830 | 862-1069 | sentence | denotes | To distinguish between the alternatives described above, we deleted an essential coding region in the mouse Capn2 gene in embryonic stems cells and transmitted this mutant allele through the mouse germline. |
| T831 | 863-865 | TO | denotes | To |
| T832 | 866-877 | VB | denotes | distinguish |
| T833 | 923-930 | VBD | denotes | deleted |
| T834 | 878-885 | IN | denotes | between |
| T835 | 886-889 | DT | denotes | the |
| T836 | 890-902 | NNS | denotes | alternatives |
| T837 | 903-912 | VBN | denotes | described |
| T838 | 913-918 | RB | denotes | above |
| T839 | 918-920 | , | denotes | , |
| T840 | 920-922 | PRP | denotes | we |
| T841 | 931-933 | DT | denotes | an |
| T842 | 951-957 | NN | denotes | region |
| T843 | 934-943 | JJ | denotes | essential |
| T844 | 944-950 | NN | denotes | coding |
| T845 | 958-960 | IN | denotes | in |
| T846 | 961-964 | DT | denotes | the |
| T847 | 977-981 | NN | denotes | gene |
| T848 | 965-970 | NN | denotes | mouse |
| T849 | 971-976 | NN | denotes | Capn2 |
| T850 | 982-984 | IN | denotes | in |
| T851 | 985-994 | JJ | denotes | embryonic |
| T852 | 1001-1006 | NNS | denotes | cells |
| T853 | 995-1000 | VBZ | denotes | stems |
| T854 | 1007-1010 | CC | denotes | and |
| T855 | 1011-1022 | VBN | denotes | transmitted |
| T856 | 1023-1027 | DT | denotes | this |
| T857 | 1035-1041 | NN | denotes | allele |
| T858 | 1028-1034 | NN | denotes | mutant |
| T859 | 1042-1049 | IN | denotes | through |
| T860 | 1050-1053 | DT | denotes | the |
| T861 | 1060-1068 | NN | denotes | germline |
| T862 | 1054-1059 | NN | denotes | mouse |
| T863 | 1068-1069 | . | denotes | . |
| T864 | 1069-1175 | sentence | denotes | Breeding of heterozygous animals failed to produce homozygous mutant live offspring or implanted embryos. |
| T865 | 1070-1078 | NN | denotes | Breeding |
| T866 | 1103-1109 | VBD | denotes | failed |
| T867 | 1079-1081 | IN | denotes | of |
| T868 | 1082-1094 | JJ | denotes | heterozygous |
| T869 | 1095-1102 | NNS | denotes | animals |
| T870 | 1110-1112 | TO | denotes | to |
| T871 | 1113-1120 | VB | denotes | produce |
| T872 | 1121-1131 | JJ | denotes | homozygous |
| T873 | 1144-1153 | NN | denotes | offspring |
| T874 | 1132-1138 | NN | denotes | mutant |
| T875 | 1139-1143 | JJ | denotes | live |
| T876 | 1154-1156 | CC | denotes | or |
| T877 | 1157-1166 | VBN | denotes | implanted |
| T878 | 1167-1174 | NNS | denotes | embryos |
| T879 | 1174-1175 | . | denotes | . |
| T880 | 1175-1332 | sentence | denotes | A nested PCR genotyping protocol was established, and homozygous preimplantation mutant embryos were detected at the morula but not at the blastocyts stage. |
| T881 | 1176-1177 | DT | denotes | A |
| T882 | 1200-1208 | NN | denotes | protocol |
| T883 | 1178-1184 | VBN | denotes | nested |
| T884 | 1185-1188 | NN | denotes | PCR |
| T885 | 1189-1199 | NN | denotes | genotyping |
| T886 | 1213-1224 | VBN | denotes | established |
| T887 | 1209-1212 | VBD | denotes | was |
| T888 | 1224-1226 | , | denotes | , |
| T889 | 1226-1229 | CC | denotes | and |
| T890 | 1230-1240 | JJ | denotes | homozygous |
| T891 | 1264-1271 | NNS | denotes | embryos |
| T892 | 1241-1256 | NN | denotes | preimplantation |
| T893 | 1257-1263 | NN | denotes | mutant |
| T894 | 1277-1285 | VBN | denotes | detected |
| T895 | 1272-1276 | VBD | denotes | were |
| T896 | 1286-1288 | IN | denotes | at |
| T897 | 1289-1292 | DT | denotes | the |
| T898 | 1293-1299 | NN | denotes | morula |
| T899 | 1300-1303 | CC | denotes | but |
| T900 | 1304-1307 | RB | denotes | not |
| T901 | 1308-1310 | IN | denotes | at |
| T902 | 1311-1314 | DT | denotes | the |
| T903 | 1315-1325 | NNS | denotes | blastocyts |
| T904 | 1326-1331 | NN | denotes | stage |
| T905 | 1331-1332 | . | denotes | . |
| T906 | 1332-1344 | sentence | denotes | Conclusion |
| T907 | 1334-1344 | NN | denotes | Conclusion |
| T908 | 1344-1490 | sentence | denotes | We conclude that homozygous disruption of the Capn2 gene results in pre-implantation embryonic lethality between the morula and blastocyst stage. |
| T909 | 1345-1347 | PRP | denotes | We |
| T910 | 1348-1356 | VBP | denotes | conclude |
| T911 | 1357-1361 | IN | denotes | that |
| T912 | 1402-1409 | VBZ | denotes | results |
| T913 | 1362-1372 | JJ | denotes | homozygous |
| T914 | 1373-1383 | NN | denotes | disruption |
| T915 | 1384-1386 | IN | denotes | of |
| T916 | 1387-1390 | DT | denotes | the |
| T917 | 1397-1401 | NN | denotes | gene |
| T918 | 1391-1396 | NN | denotes | Capn2 |
| T919 | 1410-1412 | IN | denotes | in |
| T920 | 1413-1429 | JJ | denotes | pre-implantation |
| T921 | 1440-1449 | NN | denotes | lethality |
| T922 | 1430-1439 | JJ | denotes | embryonic |
| T923 | 1450-1457 | IN | denotes | between |
| T924 | 1458-1461 | DT | denotes | the |
| T925 | 1484-1489 | NN | denotes | stage |
| T926 | 1462-1468 | NN | denotes | morula |
| T927 | 1469-1472 | CC | denotes | and |
| T928 | 1473-1483 | NN | denotes | blastocyst |
| T929 | 1489-1490 | . | denotes | . |
| T930 | 1490-1643 | sentence | denotes | This establishes that μ-calpain and m-calpain have distinct functions, and that m-calpain is vital for development of the preimplantation murine embryo. |
| T931 | 1491-1495 | DT | denotes | This |
| T932 | 1496-1507 | VBZ | denotes | establishes |
| T933 | 1508-1512 | IN | denotes | that |
| T934 | 1537-1541 | VBP | denotes | have |
| T935 | 1513-1514 | NN | denotes | μ |
| T936 | 1515-1522 | NN | denotes | calpain |
| T937 | 1514-1515 | HYPH | denotes | - |
| T938 | 1523-1526 | CC | denotes | and |
| T939 | 1527-1528 | NN | denotes | m |
| T940 | 1529-1536 | NN | denotes | calpain |
| T941 | 1528-1529 | HYPH | denotes | - |
| T942 | 1542-1550 | JJ | denotes | distinct |
| T943 | 1551-1560 | NNS | denotes | functions |
| T944 | 1560-1562 | , | denotes | , |
| T945 | 1562-1565 | CC | denotes | and |
| T946 | 1566-1570 | IN | denotes | that |
| T947 | 1581-1583 | VBZ | denotes | is |
| T948 | 1571-1572 | NN | denotes | m |
| T949 | 1573-1580 | NN | denotes | calpain |
| T950 | 1572-1573 | HYPH | denotes | - |
| T951 | 1584-1589 | JJ | denotes | vital |
| T952 | 1590-1593 | IN | denotes | for |
| T953 | 1594-1605 | NN | denotes | development |
| T954 | 1606-1608 | IN | denotes | of |
| T955 | 1609-1612 | DT | denotes | the |
| T956 | 1636-1642 | NN | denotes | embryo |
| T957 | 1613-1628 | NN | denotes | preimplantation |
| T958 | 1629-1635 | JJ | denotes | murine |
| T959 | 1642-1643 | . | denotes | . |
| T3244 | 1656-1659 | DT | denotes | The |
| T3245 | 1700-1709 | NNS | denotes | proteases |
| T3246 | 1660-1663 | CD | denotes | two |
| T3247 | 1664-1674 | JJ | denotes | ubiquitous |
| T3248 | 1675-1678 | NN | denotes | Ca2 |
| T3249 | 1680-1689 | JJ | denotes | dependent |
| T3250 | 1678-1679 | SYM | denotes | + |
| T3251 | 1679-1680 | HYPH | denotes | - |
| T3252 | 1689-1691 | , | denotes | , |
| T3253 | 1691-1699 | NN | denotes | cysteine |
| T3254 | 1767-1770 | VBP | denotes | are |
| T3255 | 1710-1715 | VBN | denotes | known |
| T3256 | 1716-1718 | IN | denotes | as |
| T3257 | 1719-1720 | NN | denotes | μ |
| T3258 | 1721-1728 | NN | denotes | calpain |
| T3259 | 1720-1721 | HYPH | denotes | - |
| T3260 | 1729-1730 | -LRB- | denotes | ( |
| T3261 | 1730-1737 | NN | denotes | calpain |
| T3262 | 1737-1738 | HYPH | denotes | - |
| T3263 | 1738-1739 | CD | denotes | 1 |
| T3264 | 1739-1740 | -RRB- | denotes | ) |
| T3265 | 1741-1744 | CC | denotes | and |
| T3266 | 1745-1746 | NN | denotes | m |
| T3267 | 1747-1754 | NN | denotes | calpain |
| T3268 | 1746-1747 | HYPH | denotes | - |
| T3269 | 1755-1756 | -LRB- | denotes | ( |
| T3270 | 1756-1762 | NN | denotes | capain |
| T3271 | 1762-1763 | HYPH | denotes | - |
| T3272 | 1763-1764 | CD | denotes | 2 |
| T3273 | 1764-1765 | -RRB- | denotes | ) |
| T3274 | 1765-1767 | , | denotes | , |
| T3275 | 1771-1774 | DT | denotes | the |
| T3276 | 1784-1791 | NNS | denotes | members |
| T3277 | 1775-1783 | NN | denotes | founding |
| T3278 | 1792-1794 | IN | denotes | of |
| T3279 | 1795-1796 | DT | denotes | a |
| T3280 | 1802-1808 | NN | denotes | family |
| T3281 | 1797-1801 | NN | denotes | gene |
| T3282 | 1809-1819 | VBG | denotes | comprising |
| T3283 | 1820-1822 | CD | denotes | 13 |
| T3284 | 1823-1828 | NNS | denotes | genes |
| T3285 | 1829-1831 | IN | denotes | in |
| T3286 | 1832-1839 | NNS | denotes | mammals |
| T3287 | 1840-1841 | -LRB- | denotes | [ |
| T3288 | 1841-1842 | CD | denotes | 1 |
| T3289 | 1842-1843 | SYM | denotes | - |
| T3290 | 1843-1844 | CD | denotes | 3 |
| T3291 | 1844-1845 | -RRB- | denotes | ] |
| T3292 | 1845-1846 | . | denotes | . |
| T3293 | 1846-2079 | sentence | denotes | Both are heterodimeric enzymes consisting of distinct 80 kDa catalytic subunits, encoded by the Capn1 (μ-80 k) and Capn2 (m-80 k) genes, respectively, that associate with a common 28 kDa regulatory subunit encoded by the Capn4 gene. |
| T3294 | 1847-1851 | DT | denotes | Both |
| T3295 | 1852-1855 | VBP | denotes | are |
| T3296 | 1856-1869 | JJ | denotes | heterodimeric |
| T3297 | 1870-1877 | NNS | denotes | enzymes |
| T3298 | 1878-1888 | VBG | denotes | consisting |
| T3299 | 1889-1891 | IN | denotes | of |
| T3300 | 1892-1900 | JJ | denotes | distinct |
| T3301 | 1918-1926 | NNS | denotes | subunits |
| T3302 | 1901-1903 | CD | denotes | 80 |
| T3303 | 1904-1907 | NN | denotes | kDa |
| T3304 | 1908-1917 | JJ | denotes | catalytic |
| T3305 | 1926-1928 | , | denotes | , |
| T3306 | 1928-1935 | VBN | denotes | encoded |
| T3307 | 1936-1938 | IN | denotes | by |
| T3308 | 1939-1942 | DT | denotes | the |
| T3309 | 1977-1982 | NNS | denotes | genes |
| T3310 | 1943-1948 | NN | denotes | Capn1 |
| T3311 | 1949-1950 | -LRB- | denotes | ( |
| T3312 | 1955-1956 | NN | denotes | k |
| T3313 | 1950-1951 | NN | denotes | μ |
| T3314 | 1951-1952 | HYPH | denotes | - |
| T3315 | 1952-1954 | CD | denotes | 80 |
| T3316 | 1956-1957 | -RRB- | denotes | ) |
| T3317 | 1958-1961 | CC | denotes | and |
| T3318 | 1962-1967 | NN | denotes | Capn2 |
| T3319 | 1968-1969 | -LRB- | denotes | ( |
| T3320 | 1974-1975 | NN | denotes | k |
| T3321 | 1969-1970 | NN | denotes | m |
| T3322 | 1970-1971 | HYPH | denotes | - |
| T3323 | 1971-1973 | CD | denotes | 80 |
| T3324 | 1975-1976 | -RRB- | denotes | ) |
| T3325 | 1982-1984 | , | denotes | , |
| T3326 | 1984-1996 | RB | denotes | respectively |
| T3327 | 1996-1998 | , | denotes | , |
| T3328 | 1998-2002 | WDT | denotes | that |
| T3329 | 2003-2012 | VBP | denotes | associate |
| T3330 | 2013-2017 | IN | denotes | with |
| T3331 | 2018-2019 | DT | denotes | a |
| T3332 | 2045-2052 | NN | denotes | subunit |
| T3333 | 2020-2026 | JJ | denotes | common |
| T3334 | 2027-2029 | CD | denotes | 28 |
| T3335 | 2030-2033 | NN | denotes | kDa |
| T3336 | 2034-2044 | JJ | denotes | regulatory |
| T3337 | 2053-2060 | VBN | denotes | encoded |
| T3338 | 2061-2063 | IN | denotes | by |
| T3339 | 2064-2067 | DT | denotes | the |
| T3340 | 2074-2078 | NN | denotes | gene |
| T3341 | 2068-2073 | NN | denotes | Capn4 |
| T3342 | 2078-2079 | . | denotes | . |
| T3343 | 2079-2249 | sentence | denotes | The μ-80 k and m-80 k subunits share 62% amino acid sequence identity, and are very similar in terms of structure, protein chemistry, and in vitro substrate specificity. |
| T3344 | 2080-2083 | DT | denotes | The |
| T3345 | 2102-2110 | NNS | denotes | subunits |
| T3346 | 2084-2085 | NN | denotes | μ |
| T3347 | 2089-2090 | NN | denotes | k |
| T3348 | 2085-2086 | HYPH | denotes | - |
| T3349 | 2086-2088 | CD | denotes | 80 |
| T3350 | 2091-2094 | CC | denotes | and |
| T3351 | 2095-2096 | NN | denotes | m |
| T3352 | 2100-2101 | NN | denotes | k |
| T3353 | 2096-2097 | HYPH | denotes | - |
| T3354 | 2097-2099 | CD | denotes | 80 |
| T3355 | 2111-2116 | VBP | denotes | share |
| T3356 | 2117-2119 | CD | denotes | 62 |
| T3357 | 2119-2120 | NN | denotes | % |
| T3358 | 2141-2149 | NN | denotes | identity |
| T3359 | 2121-2126 | NN | denotes | amino |
| T3360 | 2127-2131 | NN | denotes | acid |
| T3361 | 2132-2140 | NN | denotes | sequence |
| T3362 | 2149-2151 | , | denotes | , |
| T3363 | 2151-2154 | CC | denotes | and |
| T3364 | 2155-2158 | VBP | denotes | are |
| T3365 | 2159-2163 | RB | denotes | very |
| T3366 | 2164-2171 | JJ | denotes | similar |
| T3367 | 2172-2174 | IN | denotes | in |
| T3368 | 2175-2180 | NNS | denotes | terms |
| T3369 | 2181-2183 | IN | denotes | of |
| T3370 | 2184-2193 | NN | denotes | structure |
| T3371 | 2193-2195 | , | denotes | , |
| T3372 | 2195-2202 | NN | denotes | protein |
| T3373 | 2203-2212 | NN | denotes | chemistry |
| T3374 | 2212-2214 | , | denotes | , |
| T3375 | 2214-2217 | CC | denotes | and |
| T3376 | 2218-2220 | FW | denotes | in |
| T3377 | 2221-2226 | FW | denotes | vitro |
| T3378 | 2237-2248 | NN | denotes | specificity |
| T3379 | 2227-2236 | NN | denotes | substrate |
| T3380 | 2248-2249 | . | denotes | . |
| T3381 | 2249-2419 | sentence | denotes | Despite these similarities, the differential expression patterns of μ- and m-calpain in mammalian tissues suggest they have some isoform specific and distinct functions. |
| T3382 | 2250-2257 | IN | denotes | Despite |
| T3383 | 2356-2363 | VBP | denotes | suggest |
| T3384 | 2258-2263 | DT | denotes | these |
| T3385 | 2264-2276 | NNS | denotes | similarities |
| T3386 | 2276-2278 | , | denotes | , |
| T3387 | 2278-2281 | DT | denotes | the |
| T3388 | 2306-2314 | NNS | denotes | patterns |
| T3389 | 2282-2294 | JJ | denotes | differential |
| T3390 | 2295-2305 | NN | denotes | expression |
| T3391 | 2315-2317 | IN | denotes | of |
| T3392 | 2318-2319 | NN | denotes | μ |
| T3393 | 2327-2334 | NN | denotes | calpain |
| T3394 | 2319-2320 | HYPH | denotes | - |
| T3395 | 2321-2324 | CC | denotes | and |
| T3396 | 2325-2326 | NN | denotes | m |
| T3397 | 2326-2327 | HYPH | denotes | - |
| T3398 | 2335-2337 | IN | denotes | in |
| T3399 | 2338-2347 | JJ | denotes | mammalian |
| T3400 | 2348-2355 | NNS | denotes | tissues |
| T3401 | 2364-2368 | PRP | denotes | they |
| T3402 | 2369-2373 | VBP | denotes | have |
| T3403 | 2374-2378 | DT | denotes | some |
| T3404 | 2409-2418 | NNS | denotes | functions |
| T3405 | 2379-2386 | NN | denotes | isoform |
| T3406 | 2387-2395 | JJ | denotes | specific |
| T3407 | 2396-2399 | CC | denotes | and |
| T3408 | 2400-2408 | JJ | denotes | distinct |
| T3409 | 2418-2419 | . | denotes | . |
| T3410 | 2419-2581 | sentence | denotes | The μ and m designations derive from the levels of Ca2+ required in vitro for optimal activation; 10–50 μM Ca2+ for μ-calpain and 0.3–0.35 mM Ca2+ for m-calpain. |
| T3411 | 2420-2423 | DT | denotes | The |
| T3412 | 2432-2444 | NNS | denotes | designations |
| T3413 | 2424-2425 | NN | denotes | μ |
| T3414 | 2426-2429 | CC | denotes | and |
| T3415 | 2430-2431 | NN | denotes | m |
| T3416 | 2445-2451 | VBP | denotes | derive |
| T3417 | 2452-2456 | IN | denotes | from |
| T3418 | 2457-2460 | DT | denotes | the |
| T3419 | 2461-2467 | NNS | denotes | levels |
| T3420 | 2468-2470 | IN | denotes | of |
| T3421 | 2471-2474 | NN | denotes | Ca2 |
| T3422 | 2474-2475 | SYM | denotes | + |
| T3423 | 2476-2484 | VBN | denotes | required |
| T3424 | 2485-2487 | FW | denotes | in |
| T3425 | 2488-2493 | FW | denotes | vitro |
| T3426 | 2494-2497 | IN | denotes | for |
| T3427 | 2498-2505 | JJ | denotes | optimal |
| T3428 | 2506-2516 | NN | denotes | activation |
| T3429 | 2516-2517 | : | denotes | ; |
| T3430 | 2518-2520 | CD | denotes | 10 |
| T3431 | 2521-2523 | CD | denotes | 50 |
| T3432 | 2520-2521 | SYM | denotes | – |
| T3433 | 2524-2526 | NN | denotes | μM |
| T3434 | 2527-2530 | NN | denotes | Ca2 |
| T3435 | 2530-2531 | SYM | denotes | + |
| T3436 | 2532-2535 | IN | denotes | for |
| T3437 | 2536-2537 | NN | denotes | μ |
| T3438 | 2538-2545 | NN | denotes | calpain |
| T3439 | 2537-2538 | HYPH | denotes | - |
| T3440 | 2546-2549 | CC | denotes | and |
| T3441 | 2550-2553 | CD | denotes | 0.3 |
| T3442 | 2554-2558 | CD | denotes | 0.35 |
| T3443 | 2553-2554 | SYM | denotes | – |
| T3444 | 2559-2561 | NN | denotes | mM |
| T3445 | 2562-2565 | NN | denotes | Ca2 |
| T3446 | 2565-2566 | SYM | denotes | + |
| T3447 | 2567-2570 | IN | denotes | for |
| T3448 | 2571-2572 | NN | denotes | m |
| T3449 | 2573-2580 | NN | denotes | calpain |
| T3450 | 2572-2573 | HYPH | denotes | - |
| T3451 | 2580-2581 | . | denotes | . |
| T3452 | 2581-2743 | sentence | denotes | It is generally assumed that μ- and m-calpain maintain their differential sensitivities to calcium in vivo, although this has not yet been strictly demonstrated. |
| T3453 | 2582-2584 | PRP | denotes | It |
| T3454 | 2598-2605 | VBN | denotes | assumed |
| T3455 | 2585-2587 | VBZ | denotes | is |
| T3456 | 2588-2597 | RB | denotes | generally |
| T3457 | 2606-2610 | IN | denotes | that |
| T3458 | 2628-2636 | VBP | denotes | maintain |
| T3459 | 2611-2612 | NN | denotes | μ |
| T3460 | 2620-2627 | NN | denotes | calpain |
| T3461 | 2612-2613 | HYPH | denotes | - |
| T3462 | 2614-2617 | CC | denotes | and |
| T3463 | 2618-2619 | NN | denotes | m |
| T3464 | 2619-2620 | HYPH | denotes | - |
| T3465 | 2637-2642 | PRP$ | denotes | their |
| T3466 | 2656-2669 | NNS | denotes | sensitivities |
| T3467 | 2643-2655 | JJ | denotes | differential |
| T3468 | 2670-2672 | IN | denotes | to |
| T3469 | 2673-2680 | NN | denotes | calcium |
| T3470 | 2681-2683 | FW | denotes | in |
| T3471 | 2684-2688 | FW | denotes | vivo |
| T3472 | 2688-2690 | , | denotes | , |
| T3473 | 2690-2698 | IN | denotes | although |
| T3474 | 2730-2742 | VBN | denotes | demonstrated |
| T3475 | 2699-2703 | DT | denotes | this |
| T3476 | 2704-2707 | VBZ | denotes | has |
| T3477 | 2708-2711 | RB | denotes | not |
| T3478 | 2712-2715 | RB | denotes | yet |
| T3479 | 2716-2720 | VBN | denotes | been |
| T3480 | 2721-2729 | RB | denotes | strictly |
| T3481 | 2742-2743 | . | denotes | . |
| T3483 | 2744-2755 | RB | denotes | Furthermore |
| T3484 | 2843-2850 | VBN | denotes | assumed |
| T3485 | 2755-2757 | , | denotes | , |
| T3486 | 2757-2762 | IN | denotes | since |
| T3487 | 2803-2805 | VBZ | denotes | is |
| T3488 | 2763-2766 | DT | denotes | the |
| T3489 | 2789-2802 | NN | denotes | concentration |
| T3490 | 2767-2778 | JJ | denotes | cytoplasmic |
| T3491 | 2779-2783 | JJ | denotes | free |
| T3492 | 2784-2787 | NN | denotes | Ca2 |
| T3493 | 2787-2788 | SYM | denotes | + |
| T3494 | 2806-2815 | RB | denotes | typically |
| T3495 | 2816-2820 | JJR | denotes | less |
| T3496 | 2826-2827 | CD | denotes | 1 |
| T3497 | 2821-2825 | IN | denotes | than |
| T3498 | 2828-2830 | NN | denotes | μM |
| T3499 | 2830-2832 | , | denotes | , |
| T3500 | 2832-2834 | PRP | denotes | it |
| T3501 | 2835-2837 | VBZ | denotes | is |
| T3502 | 2838-2842 | RB | denotes | also |
| T3503 | 2851-2855 | IN | denotes | that |
| T3505 | 2856-2861 | JJ | denotes | other |
| T3507 | 2862-2864 | FW | denotes | in |
| T676 | 67-71 | NNS | denotes | mice |
| R73 | T747 | T748 | det | the,gene |
| R74 | T748 | T746 | pobj | gene,of |
| R3 | T665 | T666 | compound | m,Calpain |
| R4 | T666 | T669 | nsubjpass | Calpain,required |
| R5 | T668 | T666 | punct | -,Calpain |
| R6 | T670 | T669 | auxpass | is,required |
| R7 | T671 | T669 | prep | for,required |
| R8 | T672 | T673 | nmod | preimplantation,development |
| R9 | T673 | T671 | pobj | development,for |
| R10 | T674 | T673 | amod | embryonic,development |
| R11 | T675 | T669 | prep | in,required |
| R12 | T681 | T682 | compound | μ,calpain |
| R14 | T683 | T682 | punct | -,calpain |
| R15 | T685 | T682 | cc | and,calpain |
| R16 | T686 | T687 | compound | m,calpain |
| R18 | T688 | T687 | punct | -,calpain |
| R19 | T689 | T690 | advmod | ubiquitously,expressed |
| R20 | T690 | T691 | amod | expressed,proteases |
| R21 | T691 | T684 | attr | proteases,are |
| R22 | T692 | T691 | acl | implicated,proteases |
| R23 | T693 | T692 | prep | in,implicated |
| R24 | T694 | T695 | amod | cellular,migration |
| R25 | T695 | T693 | pobj | migration,in |
| R26 | T696 | T695 | punct | ", ",migration |
| R27 | T697 | T698 | compound | cell,cycle |
| R28 | T698 | T699 | compound | cycle,progression |
| R29 | T699 | T695 | conj | progression,migration |
| R30 | T700 | T699 | punct | ", ",progression |
| R31 | T701 | T702 | amod | degenerative,processes |
| R32 | T702 | T699 | conj | processes,progression |
| R33 | T703 | T702 | cc | and,processes |
| R34 | T704 | T705 | compound | cell,death |
| R35 | T705 | T702 | conj | death,processes |
| R36 | T706 | T684 | punct | .,are |
| R37 | T708 | T709 | det | These,enzymes |
| R39 | T710 | T709 | amod | heterodimeric,enzymes |
| R40 | T712 | T711 | auxpass | are,composed |
| R41 | T713 | T711 | prep | of,composed |
| R42 | T714 | T715 | amod | distinct,subunits |
| R44 | T716 | T715 | amod | catalytic,subunits |
| R45 | T717 | T715 | punct | ", ",subunits |
| R46 | T718 | T715 | acl | encoded,subunits |
| R47 | T719 | T718 | agent | by,encoded |
| R48 | T720 | T719 | pobj | Capn1,by |
| R49 | T721 | T720 | punct | (,Capn1 |
| R50 | T722 | T723 | compound | μ,calpain |
| R52 | T724 | T723 | punct | -,calpain |
| R53 | T725 | T720 | punct | ),Capn1 |
| R54 | T726 | T720 | cc | or,Capn1 |
| R55 | T727 | T720 | conj | Capn2,Capn1 |
| R56 | T728 | T727 | punct | (,Capn2 |
| R57 | T729 | T730 | compound | m,calpain |
| R59 | T731 | T730 | punct | -,calpain |
| R60 | T732 | T715 | punct | ),subunits |
| R61 | T733 | T715 | punct | ", ",subunits |
| R62 | T734 | T715 | cc | and,subunits |
| R63 | T735 | T736 | det | a,subunit |
| R64 | T736 | T715 | conj | subunit,subunits |
| R65 | T737 | T736 | amod | common,subunit |
| R66 | T738 | T736 | amod | regulatory,subunit |
| R67 | T739 | T736 | acl | encoded,subunit |
| R68 | T740 | T739 | agent | by,encoded |
| R69 | T741 | T740 | pobj | Capn4,by |
| R70 | T742 | T711 | punct | .,composed |
| R71 | T744 | T745 | nsubj | Disruption,abolished |
| R72 | T746 | T744 | prep | of,Disruption |
| R75 | T749 | T748 | compound | mouse,gene |
| R76 | T750 | T748 | compound | Capn4,gene |
| R77 | T751 | T752 | preconj | both,calpain |
| R78 | T752 | T755 | nmod | calpain,activity |
| R79 | T753 | T752 | nmod | μ,calpain |
| R80 | T754 | T752 | punct | -,calpain |
| R81 | T755 | T745 | dobj | activity,abolished |
| R82 | T756 | T752 | cc | and,calpain |
| R83 | T757 | T758 | compound | m,calpain |
| R84 | T758 | T752 | conj | calpain,calpain |
| R85 | T759 | T758 | punct | -,calpain |
| R86 | T760 | T745 | punct | ", ",abolished |
| R87 | T761 | T745 | cc | and,abolished |
| R88 | T762 | T745 | conj | resulted,abolished |
| R89 | T763 | T762 | prep | in,resulted |
| R90 | T764 | T765 | amod | embryonic,lethality |
| R91 | T765 | T763 | pobj | lethality,in |
| R92 | T766 | T762 | punct | ", ",resulted |
| R93 | T767 | T768 | advmod | thereby,suggesting |
| R94 | T768 | T762 | advcl | suggesting,resulted |
| R95 | T769 | T770 | amod | essential,roles |
| R96 | T770 | T768 | dobj | roles,suggesting |
| R97 | T771 | T770 | prep | for,roles |
| R98 | T772 | T771 | pobj | one,for |
| R99 | T773 | T772 | cc | or,one |
| R100 | T774 | T772 | conj | both,one |
| R101 | T775 | T772 | prep | of,one |
| R102 | T776 | T777 | det | these,enzymes |
| R103 | T777 | T775 | pobj | enzymes,of |
| R104 | T778 | T770 | prep | during,roles |
| R105 | T779 | T780 | amod | mammalian,embryogenesis |
| R106 | T780 | T778 | pobj | embryogenesis,during |
| R107 | T781 | T745 | punct | .,abolished |
| R108 | T783 | T784 | nsubj | Disruption,produced |
| R109 | T785 | T783 | prep | of,Disruption |
| R110 | T786 | T787 | det | the,gene |
| R112 | T788 | T787 | compound | Capn1,gene |
| R113 | T789 | T790 | amod | viable,mice |
| R114 | T790 | T784 | dobj | mice,produced |
| R115 | T791 | T790 | punct | ", ",mice |
| R116 | T792 | T790 | amod | fertile,mice |
| R117 | T793 | T784 | advcl | implying,produced |
| R118 | T794 | T795 | mark | that,compensate |
| R119 | T795 | T793 | advcl | compensate,implying |
| R120 | T796 | T795 | mark | either,compensate |
| R121 | T797 | T798 | compound | m,calpain |
| R122 | T798 | T795 | nsubj | calpain,compensate |
| R123 | T799 | T798 | punct | -,calpain |
| R124 | T800 | T795 | aux | could,compensate |
| R125 | T801 | T795 | prep | for,compensate |
| R126 | T802 | T803 | det | the,loss |
| R127 | T803 | T801 | pobj | loss,for |
| R128 | T804 | T803 | prep | of,loss |
| R129 | T805 | T806 | compound | μ,calpain |
| R131 | T807 | T806 | punct | -,calpain |
| R132 | T808 | T795 | punct | ", ",compensate |
| R133 | T809 | T795 | cc | or,compensate |
| R134 | T810 | T811 | mark | that,was |
| R135 | T811 | T795 | conj | was,compensate |
| R136 | T812 | T813 | det | the,loss |
| R137 | T813 | T811 | nsubj | loss,was |
| R138 | T814 | T813 | prep | of,loss |
| R139 | T815 | T816 | compound | m,calpain |
| R140 | T816 | T814 | pobj | calpain,of |
| R141 | T817 | T816 | punct | -,calpain |
| R142 | T818 | T811 | acomp | responsible,was |
| R143 | T819 | T818 | prep | for,responsible |
| R144 | T820 | T819 | pobj | death,for |
| R145 | T821 | T820 | prep | of,death |
| R146 | T822 | T823 | nmod | Capn4,mice |
| R147 | T823 | T821 | pobj | mice,of |
| R148 | T824 | T822 | punct | -,Capn4 |
| R149 | T825 | T822 | punct | /,Capn4 |
| R150 | T826 | T822 | punct | -,Capn4 |
| R151 | T827 | T784 | punct | .,produced |
| R152 | T831 | T832 | aux | To,distinguish |
| R153 | T832 | T833 | advcl | distinguish,deleted |
| R154 | T834 | T832 | prep | between,distinguish |
| R155 | T835 | T836 | det | the,alternatives |
| R156 | T836 | T834 | pobj | alternatives,between |
| R157 | T837 | T836 | acl | described,alternatives |
| R158 | T838 | T837 | advmod | above,described |
| R159 | T839 | T833 | punct | ", ",deleted |
| R160 | T840 | T833 | nsubj | we,deleted |
| R161 | T841 | T842 | det | an,region |
| R162 | T842 | T833 | dobj | region,deleted |
| R163 | T843 | T842 | amod | essential,region |
| R164 | T844 | T842 | compound | coding,region |
| R165 | T845 | T842 | prep | in,region |
| R166 | T846 | T847 | det | the,gene |
| R167 | T847 | T845 | pobj | gene,in |
| R168 | T848 | T847 | compound | mouse,gene |
| R169 | T849 | T847 | compound | Capn2,gene |
| R170 | T850 | T833 | prep | in,deleted |
| R171 | T851 | T852 | amod | embryonic,cells |
| R173 | T853 | T852 | nmod | stems,cells |
| R174 | T854 | T833 | cc | and,deleted |
| R175 | T855 | T833 | conj | transmitted,deleted |
| R176 | T856 | T857 | det | this,allele |
| R178 | T858 | T857 | compound | mutant,allele |
| R179 | T859 | T855 | prep | through,transmitted |
| R180 | T860 | T861 | det | the,germline |
| R182 | T862 | T861 | compound | mouse,germline |
| R183 | T863 | T833 | punct | .,deleted |
| R184 | T865 | T866 | nsubj | Breeding,failed |
| R185 | T867 | T865 | prep | of,Breeding |
| R186 | T868 | T869 | amod | heterozygous,animals |
| R187 | T869 | T867 | pobj | animals,of |
| R188 | T870 | T871 | aux | to,produce |
| R189 | T871 | T866 | xcomp | produce,failed |
| R190 | T872 | T873 | amod | homozygous,offspring |
| R191 | T873 | T871 | dobj | offspring,produce |
| R192 | T874 | T873 | nmod | mutant,offspring |
| R193 | T875 | T873 | amod | live,offspring |
| R194 | T876 | T873 | cc | or,offspring |
| R195 | T877 | T878 | amod | implanted,embryos |
| R196 | T878 | T873 | conj | embryos,offspring |
| R197 | T879 | T866 | punct | .,failed |
| R198 | T881 | T882 | det | A,protocol |
| R199 | T882 | T886 | nsubjpass | protocol,established |
| R200 | T883 | T882 | amod | nested,protocol |
| R201 | T884 | T882 | compound | PCR,protocol |
| R202 | T885 | T882 | compound | genotyping,protocol |
| R203 | T887 | T886 | auxpass | was,established |
| R204 | T888 | T886 | punct | ", ",established |
| R205 | T889 | T886 | cc | and,established |
| R206 | T890 | T891 | amod | homozygous,embryos |
| R207 | T891 | T894 | nsubjpass | embryos,detected |
| R208 | T892 | T891 | compound | preimplantation,embryos |
| R209 | T893 | T891 | compound | mutant,embryos |
| R211 | T895 | T894 | auxpass | were,detected |
| R212 | T896 | T894 | prep | at,detected |
| R213 | T897 | T898 | det | the,morula |
| R214 | T898 | T896 | pobj | morula,at |
| R215 | T899 | T894 | cc | but,detected |
| R216 | T900 | T894 | conj | not,detected |
| R217 | T901 | T900 | prep | at,not |
| R218 | T902 | T903 | det | the,blastocyts |
| R219 | T903 | T901 | pobj | blastocyts,at |
| R220 | T904 | T900 | dobj | stage,not |
| R221 | T905 | T894 | punct | .,detected |
| R222 | T909 | T910 | nsubj | We,conclude |
| R223 | T911 | T912 | mark | that,results |
| R224 | T912 | T910 | ccomp | results,conclude |
| R225 | T913 | T914 | amod | homozygous,disruption |
| R226 | T914 | T912 | nsubj | disruption,results |
| R227 | T915 | T914 | prep | of,disruption |
| R228 | T916 | T917 | det | the,gene |
| R229 | T917 | T915 | pobj | gene,of |
| R230 | T918 | T917 | compound | Capn2,gene |
| R231 | T919 | T912 | prep | in,results |
| R232 | T920 | T921 | amod | pre-implantation,lethality |
| R234 | T922 | T921 | amod | embryonic,lethality |
| R235 | T923 | T912 | prep | between,results |
| R236 | T924 | T925 | det | the,stage |
| R237 | T925 | T923 | pobj | stage,between |
| R238 | T926 | T925 | nmod | morula,stage |
| R239 | T927 | T926 | cc | and,morula |
| R240 | T928 | T926 | conj | blastocyst,morula |
| R241 | T929 | T910 | punct | .,conclude |
| R242 | T931 | T932 | nsubj | This,establishes |
| R243 | T933 | T934 | mark | that,have |
| R244 | T934 | T932 | advcl | have,establishes |
| R245 | T935 | T936 | compound | μ,calpain |
| R246 | T936 | T934 | nsubj | calpain,have |
| R247 | T937 | T936 | punct | -,calpain |
| R248 | T938 | T936 | cc | and,calpain |
| R249 | T939 | T940 | compound | m,calpain |
| R250 | T940 | T936 | conj | calpain,calpain |
| R251 | T941 | T940 | punct | -,calpain |
| R252 | T942 | T943 | amod | distinct,functions |
| R253 | T943 | T934 | dobj | functions,have |
| R254 | T944 | T934 | punct | ", ",have |
| R255 | T945 | T934 | cc | and,have |
| R256 | T946 | T947 | mark | that,is |
| R257 | T947 | T934 | conj | is,have |
| R258 | T948 | T949 | compound | m,calpain |
| R259 | T949 | T947 | nsubj | calpain,is |
| R260 | T950 | T949 | punct | -,calpain |
| R261 | T951 | T947 | acomp | vital,is |
| R262 | T952 | T951 | prep | for,vital |
| R263 | T953 | T952 | pobj | development,for |
| R264 | T954 | T953 | prep | of,development |
| R265 | T955 | T956 | det | the,embryo |
| R266 | T956 | T954 | pobj | embryo,of |
| R267 | T957 | T956 | nmod | preimplantation,embryo |
| R268 | T958 | T956 | amod | murine,embryo |
| R269 | T959 | T932 | punct | .,establishes |
| R270 | T676 | T675 | pobj | mice,in |
| R282 | T3323 | T3320 | nummod | 80,k |
| R287 | T3324 | T3320 | punct | ),k |
| R288 | T3325 | T3306 | punct | ", ",encoded |
| R289 | T3326 | T3306 | advmod | respectively,encoded |
| R290 | T3244 | T3245 | det | The,proteases |
| R291 | T3327 | T3301 | punct | ", ",subunits |
| R292 | T3245 | T3254 | nsubj | proteases,are |
| R293 | T3246 | T3245 | nummod | two,proteases |
| R294 | T3328 | T3329 | dep | that,associate |
| R295 | T3247 | T3245 | amod | ubiquitous,proteases |
| R296 | T3248 | T3249 | npadvmod | Ca2,dependent |
| R297 | T3329 | T3301 | relcl | associate,subunits |
| R298 | T3249 | T3245 | amod | dependent,proteases |
| R299 | T3250 | T3248 | punct | +,Ca2 |
| R300 | T3251 | T3249 | punct | -,dependent |
| R301 | T3330 | T3329 | prep | with,associate |
| R302 | T3252 | T3245 | punct | ", ",proteases |
| R303 | T3253 | T3245 | compound | cysteine,proteases |
| R304 | T3255 | T3245 | acl | known,proteases |
| R305 | T3331 | T3332 | det | a,subunit |
| R306 | T3256 | T3255 | prep | as,known |
| R307 | T3257 | T3258 | compound | μ,calpain |
| R308 | T3258 | T3256 | pobj | calpain,as |
| R309 | T3332 | T3330 | pobj | subunit,with |
| R310 | T3259 | T3258 | punct | -,calpain |
| R311 | T3260 | T3258 | punct | (,calpain |
| R312 | T3261 | T3258 | appos | calpain,calpain |
| R313 | T3333 | T3332 | amod | common,subunit |
| R314 | T3262 | T3261 | punct | -,calpain |
| R315 | T3263 | T3261 | nummod | 1,calpain |
| R316 | T3264 | T3258 | punct | ),calpain |
| R317 | T3334 | T3335 | nummod | 28,kDa |
| R318 | T3265 | T3258 | cc | and,calpain |
| R319 | T3335 | T3332 | nmod | kDa,subunit |
| R320 | T3266 | T3267 | compound | m,calpain |
| R321 | T3267 | T3258 | conj | calpain,calpain |
| R322 | T3268 | T3267 | punct | -,calpain |
| R323 | T3269 | T3267 | punct | (,calpain |
| R324 | T3270 | T3267 | appos | capain,calpain |
| R325 | T3271 | T3270 | punct | -,capain |
| R326 | T3272 | T3270 | nummod | 2,capain |
| R327 | T3273 | T3254 | punct | ),are |
| R328 | T3274 | T3254 | punct | ", ",are |
| R329 | T3275 | T3276 | det | the,members |
| R330 | T3276 | T3254 | attr | members,are |
| R331 | T3277 | T3276 | compound | founding,members |
| R332 | T3278 | T3276 | prep | of,members |
| R333 | T3336 | T3332 | amod | regulatory,subunit |
| R334 | T3279 | T3280 | det | a,family |
| R335 | T3280 | T3278 | pobj | family,of |
| R336 | T3281 | T3280 | compound | gene,family |
| R337 | T3282 | T3280 | acl | comprising,family |
| R338 | T3337 | T3332 | acl | encoded,subunit |
| R339 | T3283 | T3284 | nummod | 13,genes |
| R340 | T3284 | T3282 | dobj | genes,comprising |
| R341 | T3285 | T3282 | prep | in,comprising |
| R342 | T3338 | T3337 | agent | by,encoded |
| R343 | T3286 | T3285 | pobj | mammals,in |
| R344 | T3287 | T3288 | punct | [,1 |
| R345 | T3288 | T3254 | parataxis | 1,are |
| R346 | T3339 | T3340 | det | the,gene |
| R347 | T3289 | T3290 | punct | -,3 |
| R348 | T3290 | T3288 | prep | 3,1 |
| R349 | T3291 | T3288 | punct | ],1 |
| R350 | T3340 | T3338 | pobj | gene,by |
| R351 | T3292 | T3254 | punct | .,are |
| R352 | T3341 | T3340 | compound | Capn4,gene |
| R353 | T3294 | T3295 | nsubj | Both,are |
| R354 | T3296 | T3297 | amod | heterodimeric,enzymes |
| R355 | T3342 | T3295 | punct | .,are |
| R356 | T3297 | T3295 | attr | enzymes,are |
| R357 | T3298 | T3297 | acl | consisting,enzymes |
| R358 | T3299 | T3298 | prep | of,consisting |
| R359 | T3344 | T3345 | det | The,subunits |
| R360 | T3300 | T3301 | amod | distinct,subunits |
| R361 | T3301 | T3299 | pobj | subunits,of |
| R362 | T3302 | T3303 | nummod | 80,kDa |
| R363 | T3303 | T3301 | nmod | kDa,subunits |
| R364 | T3345 | T3355 | nsubj | subunits,share |
| R365 | T3304 | T3301 | amod | catalytic,subunits |
| R366 | T3305 | T3301 | punct | ", ",subunits |
| R367 | T3346 | T3347 | nmod | μ,k |
| R368 | T3347 | T3345 | nmod | k,subunits |
| R369 | T3306 | T3301 | acl | encoded,subunits |
| R370 | T3307 | T3306 | agent | by,encoded |
| R371 | T3348 | T3346 | punct | -,μ |
| R372 | T3308 | T3309 | det | the,genes |
| R373 | T3309 | T3307 | pobj | genes,by |
| R374 | T3310 | T3309 | nmod | Capn1,genes |
| R375 | T3311 | T3312 | punct | (,k |
| R376 | T3349 | T3346 | nummod | 80,μ |
| R377 | T3312 | T3310 | parataxis | k,Capn1 |
| R378 | T3313 | T3312 | nmod | μ,k |
| R379 | T3314 | T3312 | punct | -,k |
| R380 | T3315 | T3312 | nummod | 80,k |
| R381 | T3350 | T3347 | cc | and,k |
| R382 | T3316 | T3312 | punct | ),k |
| R383 | T3317 | T3310 | cc | and,Capn1 |
| R384 | T3318 | T3310 | conj | Capn2,Capn1 |
| R385 | T3351 | T3352 | nmod | m,k |
| R386 | T3319 | T3320 | punct | (,k |
| R387 | T3320 | T3318 | parataxis | k,Capn2 |
| R388 | T3321 | T3320 | nmod | m,k |
| R389 | T3352 | T3347 | conj | k,k |
| R390 | T3322 | T3320 | punct | -,k |
| R391 | T3353 | T3351 | punct | -,m |
| R392 | T3354 | T3351 | nummod | 80,m |
| R393 | T3356 | T3357 | nummod | 62,% |
| R394 | T3429 | T3419 | punct | ;,levels |
| R395 | T3357 | T3358 | compound | %,identity |
| R396 | T3430 | T3431 | quantmod | 10,50 |
| R397 | T3431 | T3433 | nummod | 50,μM |
| R398 | T3358 | T3355 | dobj | identity,share |
| R399 | T3432 | T3431 | punct | –,50 |
| R400 | T3433 | T3434 | compound | μM,Ca2 |
| R401 | T3434 | T3419 | appos | Ca2,levels |
| R402 | T3359 | T3360 | compound | amino,acid |
| R403 | T3435 | T3434 | punct | +,Ca2 |
| R404 | T3436 | T3434 | prep | for,Ca2 |
| R405 | T3437 | T3438 | compound | μ,calpain |
| R406 | T3360 | T3358 | compound | acid,identity |
| R407 | T3438 | T3436 | pobj | calpain,for |
| R408 | T3439 | T3438 | punct | -,calpain |
| R409 | T3440 | T3434 | cc | and,Ca2 |
| R410 | T3361 | T3358 | compound | sequence,identity |
| R411 | T3441 | T3442 | quantmod | 0.3,0.35 |
| R412 | T3442 | T3444 | nummod | 0.35,mM |
| R413 | T3443 | T3442 | punct | –,0.35 |
| R414 | T3362 | T3355 | punct | ", ",share |
| R415 | T3444 | T3445 | compound | mM,Ca2 |
| R416 | T3445 | T3434 | conj | Ca2,Ca2 |
| R417 | T3363 | T3355 | cc | and,share |
| R418 | T3446 | T3445 | punct | +,Ca2 |
| R419 | T3447 | T3445 | prep | for,Ca2 |
| R420 | T3448 | T3449 | compound | m,calpain |
| R421 | T3449 | T3447 | pobj | calpain,for |
| R422 | T3364 | T3355 | conj | are,share |
| R423 | T3450 | T3449 | punct | -,calpain |
| R424 | T3451 | T3416 | punct | .,derive |
| R425 | T3453 | T3454 | nsubjpass | It,assumed |
| R426 | T3365 | T3366 | advmod | very,similar |
| R427 | T3455 | T3454 | auxpass | is,assumed |
| R428 | T3456 | T3454 | advmod | generally,assumed |
| R429 | T3457 | T3458 | mark | that,maintain |
| R430 | T3366 | T3364 | acomp | similar,are |
| R431 | T3458 | T3454 | ccomp | maintain,assumed |
| R432 | T3459 | T3460 | nmod | μ,calpain |
| R433 | T3367 | T3364 | prep | in,are |
| R434 | T3460 | T3458 | nsubj | calpain,maintain |
| R435 | T3461 | T3459 | punct | -,μ |
| R436 | T3462 | T3459 | cc | and,μ |
| R437 | T3463 | T3459 | conj | m,μ |
| R438 | T3368 | T3367 | pobj | terms,in |
| R439 | T3464 | T3460 | punct | -,calpain |
| R440 | T3465 | T3466 | poss | their,sensitivities |
| R441 | T3466 | T3458 | dobj | sensitivities,maintain |
| R442 | T3369 | T3368 | prep | of,terms |
| R443 | T3467 | T3466 | amod | differential,sensitivities |
| R444 | T3468 | T3466 | prep | to,sensitivities |
| R445 | T3370 | T3369 | pobj | structure,of |
| R446 | T3469 | T3468 | pobj | calcium,to |
| R447 | T3470 | T3471 | advmod | in,vivo |
| R448 | T3471 | T3458 | advmod | vivo,maintain |
| R449 | T3371 | T3370 | punct | ", ",structure |
| R450 | T3472 | T3458 | punct | ", ",maintain |
| R451 | T3473 | T3474 | mark | although,demonstrated |
| R452 | T3372 | T3373 | compound | protein,chemistry |
| R453 | T3373 | T3370 | conj | chemistry,structure |
| R454 | T3474 | T3458 | advcl | demonstrated,maintain |
| R455 | T3475 | T3474 | nsubjpass | this,demonstrated |
| R456 | T3476 | T3474 | aux | has,demonstrated |
| R457 | T3374 | T3373 | punct | ", ",chemistry |
| R458 | T3477 | T3474 | neg | not,demonstrated |
| R459 | T3478 | T3474 | advmod | yet,demonstrated |
| R460 | T3375 | T3373 | cc | and,chemistry |
| R461 | T3479 | T3474 | auxpass | been,demonstrated |
| R462 | T3480 | T3474 | advmod | strictly,demonstrated |
| R463 | T3481 | T3454 | punct | .,assumed |
| R464 | T3376 | T3377 | advmod | in,vitro |
| R465 | T3483 | T3484 | advmod | Furthermore,assumed |
| R466 | T3377 | T3378 | amod | vitro,specificity |
| R467 | T3485 | T3484 | punct | ", ",assumed |
| R468 | T3486 | T3487 | mark | since,is |
| R469 | T3378 | T3373 | conj | specificity,chemistry |
| R470 | T3487 | T3484 | advcl | is,assumed |
| R471 | T3488 | T3489 | det | the,concentration |
| R472 | T3379 | T3378 | compound | substrate,specificity |
| R473 | T3489 | T3487 | nsubj | concentration,is |
| R474 | T3490 | T3491 | amod | cytoplasmic,free |
| R475 | T3491 | T3489 | amod | free,concentration |
| R476 | T3380 | T3355 | punct | .,share |
| R477 | T3492 | T3489 | nmod | Ca2,concentration |
| R478 | T3493 | T3492 | punct | +,Ca2 |
| R479 | T3494 | T3487 | advmod | typically,is |
| R480 | T3495 | T3496 | amod | less,1 |
| R481 | T3496 | T3498 | nummod | 1,μM |
| R482 | T3497 | T3496 | quantmod | than,1 |
| R483 | T3382 | T3383 | prep | Despite,suggest |
| R484 | T3498 | T3487 | attr | μM,is |
| R485 | T3499 | T3484 | punct | ", ",assumed |
| R486 | T3500 | T3484 | nsubjpass | it,assumed |
| R487 | T3501 | T3484 | auxpass | is,assumed |
| R488 | T3384 | T3385 | det | these,similarities |
| R489 | T3502 | T3484 | advmod | also,assumed |
| R494 | T3385 | T3382 | pobj | similarities,Despite |
| R499 | T3386 | T3383 | punct | ", ",suggest |
| R502 | T3387 | T3388 | det | the,patterns |
| R506 | T3388 | T3383 | nsubj | patterns,suggest |
| R511 | T3389 | T3388 | amod | differential,patterns |
| R514 | T3390 | T3388 | compound | expression,patterns |
| R518 | T3391 | T3388 | prep | of,patterns |
| R522 | T3392 | T3393 | nmod | μ,calpain |
| R526 | T3393 | T3391 | pobj | calpain,of |
| R529 | T3394 | T3392 | punct | -,μ |
| R531 | T3395 | T3392 | cc | and,μ |
| R536 | T3396 | T3392 | conj | m,μ |
| R540 | T3397 | T3393 | punct | -,calpain |
| R542 | T3398 | T3388 | prep | in,patterns |
| R545 | T3399 | T3400 | amod | mammalian,tissues |
| R549 | T3400 | T3398 | pobj | tissues,in |
| R552 | T3401 | T3402 | nsubj | they,have |
| R556 | T3402 | T3383 | advcl | have,suggest |
| R560 | T3403 | T3404 | det | some,functions |
| R562 | T3404 | T3402 | dobj | functions,have |
| R564 | T3405 | T3406 | npadvmod | isoform,specific |
| R568 | T3406 | T3404 | amod | specific,functions |
| R570 | T3407 | T3406 | cc | and,specific |
| R572 | T3408 | T3406 | conj | distinct,specific |
| R575 | T3409 | T3383 | punct | .,suggest |
| R578 | T3411 | T3412 | det | The,designations |
| R586 | T3412 | T3416 | nsubj | designations,derive |
| R589 | T3413 | T3412 | nmod | μ,designations |
| R593 | T3414 | T3413 | cc | and,μ |
| R597 | T3415 | T3413 | conj | m,μ |
| R601 | T3417 | T3416 | prep | from,derive |
| R606 | T3418 | T3419 | det | the,levels |
| R610 | T3419 | T3417 | pobj | levels,from |
| R615 | T3420 | T3419 | prep | of,levels |
| R618 | T3421 | T3420 | pobj | Ca2,of |
| R622 | T3422 | T3421 | punct | +,Ca2 |
| R626 | T3423 | T3419 | acl | required,levels |
| R630 | T3424 | T3425 | advmod | in,vitro |
| R637 | T3425 | T3423 | advmod | vitro,required |
| R640 | T3426 | T3423 | prep | for,required |
| R645 | T3427 | T3428 | amod | optimal,activation |
| R649 | T3428 | T3426 | pobj | activation,for |
| R13 | T682 | T684 | nsubj | calpain,are |
| R17 | T687 | T682 | conj | calpain,calpain |
| R38 | T709 | T711 | nsubjpass | enzymes,composed |
| R43 | T715 | T713 | pobj | subunits,of |
| R51 | T723 | T720 | appos | calpain,Capn1 |
| R58 | T730 | T727 | appos | calpain,Capn2 |
| R111 | T787 | T785 | pobj | gene,of |
| R130 | T806 | T804 | pobj | calpain,of |
| R172 | T852 | T850 | pobj | cells,in |
| R177 | T857 | T855 | dobj | allele,transmitted |
| R181 | T861 | T859 | pobj | germline,through |
| R210 | T894 | T886 | conj | detected,established |
| R233 | T921 | T919 | pobj | lethality,in |