PMC:1359074 / 38171-38849 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1359074","sourcedb":"PMC","sourceid":"1359074","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1359074","text":"Histology.\n18.5-dpc embryos were exsanguinated and peripheral blood smears were prepared from both mutant and WT mice. The slides were Wright-stained and read by DAF. For whole mount analysis, 14.5-dpc WT and mutant embryos, and postnatal day–10.5 mice were fixed in 10% formalin, paraffin-embedded, sectioned at 5 μm, and stained with hematoxylin and eosin. Liver samples (at 14.5 dpc and 18.5 dpc) were prepared in a similar manner. Images were obtained with Nikon Labophot-2 microscope. Objectives used were E Plan 40/0.65 160/0.17 Nikon (40× objective), E Plan 100/1.25 oil 160/0.17 Nikon (100× objective). The camera was a Nikon Coolpix 4300. 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