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pmc-enju-pas

bionlp-st-ge-2016-test-proteins

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T9829","span":{"begin":990,"end":994},"obj":"Protein"},{"id":"T9828","span":{"begin":882,"end":891},"obj":"Protein"},{"id":"T9827","span":{"begin":722,"end":726},"obj":"Protein"},{"id":"T9826","span":{"begin":607,"end":611},"obj":"Protein"},{"id":"T9825","span":{"begin":424,"end":428},"obj":"Protein"},{"id":"T9824","span":{"begin":333,"end":337},"obj":"Protein"},{"id":"T9823","span":{"begin":212,"end":216},"obj":"Protein"},{"id":"T9822","span":{"begin":137,"end":146},"obj":"Protein"},{"id":"T9821","span":{"begin":97,"end":101},"obj":"Protein"},{"id":"T9820","span":{"begin":26,"end":30},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T10037","span":{"begin":990,"end":994},"obj":"http://www.uniprot.org/uniprot/P35712"},{"id":"T10036","span":{"begin":722,"end":726},"obj":"http://www.uniprot.org/uniprot/P35712"},{"id":"T10035","span":{"begin":607,"end":611},"obj":"http://www.uniprot.org/uniprot/P35712"},{"id":"T10034","span":{"begin":424,"end":428},"obj":"http://www.uniprot.org/uniprot/P35712"},{"id":"T10033","span":{"begin":333,"end":337},"obj":"http://www.uniprot.org/uniprot/P35712"},{"id":"T10032","span":{"begin":212,"end":216},"obj":"http://www.uniprot.org/uniprot/P35712"},{"id":"T10031","span":{"begin":97,"end":101},"obj":"http://www.uniprot.org/uniprot/P35712"},{"id":"T10030","span":{"begin":26,"end":30},"obj":"http://www.uniprot.org/uniprot/P35712"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T9781","span":{"begin":673,"end":686},"obj":"http://purl.obolibrary.org/obo/UBERON_0003061"},{"id":"T9780","span":{"begin":673,"end":678},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T9779","span":{"begin":669,"end":672},"obj":"http://purl.obolibrary.org/obo/UBERON_0009856"},{"id":"T9778","span":{"begin":664,"end":686},"obj":"http://purl.obolibrary.org/obo/UBERON_0011919"},{"id":"T9777","span":{"begin":664,"end":672},"obj":"http://purl.obolibrary.org/obo/UBERON_0001040"},{"id":"T9776","span":{"begin":664,"end":668},"obj":"http://purl.obolibrary.org/obo/UBERON_2000084"},{"id":"T9775","span":{"begin":919,"end":924},"obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"id":"T9774","span":{"begin":646,"end":651},"obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"id":"T9773","span":{"begin":554,"end":559},"obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"id":"T9772","span":{"begin":150,"end":155},"obj":"http://purl.obolibrary.org/obo/UBERON_0002107"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T9800","span":{"begin":802,"end":827},"obj":"http://purl.obolibrary.org/obo/GO_0060319"},{"id":"T9799","span":{"begin":885,"end":891},"obj":"http://purl.obolibrary.org/obo/GO_0005344"},{"id":"T9798","span":{"begin":140,"end":146},"obj":"http://purl.obolibrary.org/obo/GO_0005344"},{"id":"T9797","span":{"begin":1019,"end":1033},"obj":"http://purl.obolibrary.org/obo/GO_0030218"},{"id":"T9796","span":{"begin":813,"end":827},"obj":"http://purl.obolibrary.org/obo/GO_0030218"},{"id":"T9795","span":{"begin":532,"end":546},"obj":"http://purl.obolibrary.org/obo/GO_0030218"},{"id":"T9794","span":{"begin":257,"end":271},"obj":"http://purl.obolibrary.org/obo/GO_0030218"},{"id":"T9793","span":{"begin":61,"end":75},"obj":"http://purl.obolibrary.org/obo/GO_0030218"},{"id":"T9792","span":{"begin":1008,"end":1033},"obj":"http://purl.obolibrary.org/obo/GO_0060318"},{"id":"T9791","span":{"begin":521,"end":546},"obj":"http://purl.obolibrary.org/obo/GO_0060318"},{"id":"T9790","span":{"begin":246,"end":271},"obj":"http://purl.obolibrary.org/obo/GO_0060318"},{"id":"T9789","span":{"begin":50,"end":75},"obj":"http://purl.obolibrary.org/obo/GO_0060318"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T9831","span":{"begin":885,"end":891},"obj":"http://purl.obolibrary.org/obo/GO_0005344"},{"id":"T9830","span":{"begin":140,"end":146},"obj":"http://purl.obolibrary.org/obo/GO_0005344"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T9834","span":{"begin":664,"end":668},"obj":"http://purl.obolibrary.org/obo/GO_0060417"},{"id":"T9833","span":{"begin":925,"end":930},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T9832","span":{"begin":184,"end":189},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

sentences

{"project":"sentences","denotations":[{"id":"T9788","span":{"begin":829,"end":1034},"obj":"Sentence"},{"id":"T9787","span":{"begin":711,"end":828},"obj":"Sentence"},{"id":"T9786","span":{"begin":561,"end":710},"obj":"Sentence"},{"id":"T9785","span":{"begin":401,"end":560},"obj":"Sentence"},{"id":"T9784","span":{"begin":273,"end":400},"obj":"Sentence"},{"id":"T9783","span":{"begin":76,"end":272},"obj":"Sentence"},{"id":"T9782","span":{"begin":0,"end":75},"obj":"Sentence"},{"id":"T167","span":{"begin":0,"end":75},"obj":"Sentence"},{"id":"T168","span":{"begin":76,"end":272},"obj":"Sentence"},{"id":"T169","span":{"begin":273,"end":400},"obj":"Sentence"},{"id":"T170","span":{"begin":401,"end":560},"obj":"Sentence"},{"id":"T171","span":{"begin":561,"end":710},"obj":"Sentence"},{"id":"T172","span":{"begin":711,"end":828},"obj":"Sentence"},{"id":"T173","span":{"begin":829,"end":1034},"obj":"Sentence"},{"id":"T174","span":{"begin":1035,"end":1107},"obj":"Sentence"},{"id":"T175","span":{"begin":1108,"end":1180},"obj":"Sentence"},{"id":"T176","span":{"begin":1181,"end":1277},"obj":"Sentence"},{"id":"T177","span":{"begin":1278,"end":1379},"obj":"Sentence"},{"id":"T178","span":{"begin":1380,"end":1445},"obj":"Sentence"},{"id":"T179","span":{"begin":1446,"end":1497},"obj":"Sentence"},{"id":"T180","span":{"begin":1498,"end":1506},"obj":"Sentence"},{"id":"T181","span":{"begin":1507,"end":1681},"obj":"Sentence"},{"id":"T182","span":{"begin":1682,"end":1804},"obj":"Sentence"},{"id":"T183","span":{"begin":1805,"end":1814},"obj":"Sentence"},{"id":"T184","span":{"begin":1815,"end":1872},"obj":"Sentence"},{"id":"T185","span":{"begin":1873,"end":1935},"obj":"Sentence"},{"id":"T186","span":{"begin":1936,"end":2049},"obj":"Sentence"},{"id":"T187","span":{"begin":2050,"end":2081},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

simple1

{"project":"simple1","denotations":[{"id":"T9861","span":{"begin":990,"end":994},"obj":"Protein"},{"id":"T9860","span":{"begin":882,"end":891},"obj":"Protein"},{"id":"T9859","span":{"begin":722,"end":726},"obj":"Protein"},{"id":"T9858","span":{"begin":607,"end":611},"obj":"Protein"},{"id":"T9857","span":{"begin":424,"end":428},"obj":"Protein"},{"id":"T9856","span":{"begin":333,"end":337},"obj":"Protein"},{"id":"T9855","span":{"begin":212,"end":216},"obj":"Protein"},{"id":"T9854","span":{"begin":137,"end":146},"obj":"Protein"},{"id":"T9853","span":{"begin":97,"end":101},"obj":"Protein"},{"id":"T9852","span":{"begin":26,"end":30},"obj":"Protein"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

BioNLP16_DUT

{"project":"BioNLP16_DUT","denotations":[{"id":"T10321","span":{"begin":902,"end":911},"obj":"Gene_expression"},{"id":"T10320","span":{"begin":727,"end":737},"obj":"Gene_expression"},{"id":"T10319","span":{"begin":622,"end":633},"obj":"Transcription"},{"id":"T10318","span":{"begin":311,"end":321},"obj":"Gene_expression"},{"id":"T10317","span":{"begin":129,"end":136},"obj":"Gene_expression"},{"id":"T10316","span":{"begin":102,"end":111},"obj":"Negative_regulation"},{"id":"T10315","span":{"begin":4,"end":14},"obj":"Gene_expression"},{"id":"T10314","span":{"begin":882,"end":891},"obj":"Protein"},{"id":"T10313","span":{"begin":990,"end":994},"obj":"Protein"},{"id":"T10312","span":{"begin":722,"end":726},"obj":"Protein"},{"id":"T10311","span":{"begin":607,"end":611},"obj":"Protein"},{"id":"T10310","span":{"begin":424,"end":428},"obj":"Protein"},{"id":"T10309","span":{"begin":333,"end":337},"obj":"Protein"},{"id":"T10308","span":{"begin":212,"end":216},"obj":"Protein"},{"id":"T10307","span":{"begin":137,"end":146},"obj":"Protein"},{"id":"T10306","span":{"begin":97,"end":101},"obj":"Protein"},{"id":"T10305","span":{"begin":26,"end":30},"obj":"Protein"}],"relations":[{"id":"R7152","pred":"themeOf","subj":"T10305","obj":"T10315"},{"id":"R7153","pred":"themeOf","subj":"T10306","obj":"T10316"},{"id":"R7154","pred":"themeOf","subj":"T10307","obj":"T10317"},{"id":"R7155","pred":"themeOf","subj":"T10309","obj":"T10318"},{"id":"R7156","pred":"themeOf","subj":"T10311","obj":"T10319"},{"id":"R7157","pred":"themeOf","subj":"T10312","obj":"T10320"},{"id":"R7158","pred":"themeOf","subj":"T10314","obj":"T10321"},{"id":"R7159","pred":"themeOf","subj":"T10317","obj":"T10316"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

BioNLP16_Messiy

{"project":"BioNLP16_Messiy","denotations":[{"id":"T10053","span":{"begin":902,"end":911},"obj":"Gene_expression"},{"id":"T10052","span":{"begin":727,"end":737},"obj":"Gene_expression"},{"id":"T10051","span":{"begin":311,"end":321},"obj":"Gene_expression"},{"id":"T10050","span":{"begin":102,"end":111},"obj":"Negative_regulation"},{"id":"T10049","span":{"begin":129,"end":136},"obj":"Gene_expression"},{"id":"T10048","span":{"begin":4,"end":14},"obj":"Gene_expression"},{"id":"T10047","span":{"begin":882,"end":891},"obj":"Protein"},{"id":"T10046","span":{"begin":990,"end":994},"obj":"Protein"},{"id":"T10045","span":{"begin":722,"end":726},"obj":"Protein"},{"id":"T10044","span":{"begin":607,"end":611},"obj":"Protein"},{"id":"T10043","span":{"begin":424,"end":428},"obj":"Protein"},{"id":"T10042","span":{"begin":333,"end":337},"obj":"Protein"},{"id":"T10041","span":{"begin":212,"end":216},"obj":"Protein"},{"id":"T10040","span":{"begin":137,"end":146},"obj":"Protein"},{"id":"T10039","span":{"begin":97,"end":101},"obj":"Protein"},{"id":"T10038","span":{"begin":26,"end":30},"obj":"Protein"}],"relations":[{"id":"R6951","pred":"themeOf","subj":"T10038","obj":"T10048"},{"id":"R6952","pred":"themeOf","subj":"T10039","obj":"T10050"},{"id":"R6953","pred":"themeOf","subj":"T10040","obj":"T10049"},{"id":"R6954","pred":"themeOf","subj":"T10042","obj":"T10051"},{"id":"R6955","pred":"themeOf","subj":"T10045","obj":"T10052"},{"id":"R6956","pred":"themeOf","subj":"T10047","obj":"T10053"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

DLUT931

{"project":"DLUT931","denotations":[{"id":"T10079","span":{"begin":902,"end":911},"obj":"Gene_expression"},{"id":"T10078","span":{"begin":727,"end":737},"obj":"Gene_expression"},{"id":"T10077","span":{"begin":311,"end":321},"obj":"Gene_expression"},{"id":"T10076","span":{"begin":129,"end":136},"obj":"Gene_expression"},{"id":"T10075","span":{"begin":102,"end":111},"obj":"Negative_regulation"},{"id":"T10074","span":{"begin":4,"end":14},"obj":"Gene_expression"},{"id":"T10073","span":{"begin":882,"end":891},"obj":"Protein"},{"id":"T10072","span":{"begin":990,"end":994},"obj":"Protein"},{"id":"T10071","span":{"begin":722,"end":726},"obj":"Protein"},{"id":"T10070","span":{"begin":607,"end":611},"obj":"Protein"},{"id":"T10069","span":{"begin":424,"end":428},"obj":"Protein"},{"id":"T10068","span":{"begin":333,"end":337},"obj":"Protein"},{"id":"T10067","span":{"begin":212,"end":216},"obj":"Protein"},{"id":"T10066","span":{"begin":137,"end":146},"obj":"Protein"},{"id":"T10065","span":{"begin":97,"end":101},"obj":"Protein"},{"id":"T10064","span":{"begin":26,"end":30},"obj":"Protein"}],"relations":[{"id":"R6957","pred":"themeOf","subj":"T10064","obj":"T10074"},{"id":"R6958","pred":"themeOf","subj":"T10065","obj":"T10075"},{"id":"R6959","pred":"themeOf","subj":"T10066","obj":"T10076"},{"id":"R6960","pred":"themeOf","subj":"T10068","obj":"T10077"},{"id":"R6961","pred":"themeOf","subj":"T10071","obj":"T10078"},{"id":"R6962","pred":"themeOf","subj":"T10073","obj":"T10079"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

bionlp-st-ge-2016-test-ihmc

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T10304","span":{"begin":722,"end":737},"obj":"Gene_expression"},{"id":"T10303","span":{"begin":574,"end":709},"obj":"Transcription"},{"id":"T10302","span":{"begin":722,"end":726},"obj":"Protein"},{"id":"T10301","span":{"begin":137,"end":146},"obj":"Protein"},{"id":"T10300","span":{"begin":26,"end":30},"obj":"Protein"},{"id":"T10299","span":{"begin":934,"end":971},"obj":"Protein"},{"id":"T10298","span":{"begin":424,"end":428},"obj":"Protein"},{"id":"T10297","span":{"begin":163,"end":189},"obj":"Entity"},{"id":"T10296","span":{"begin":669,"end":672},"obj":"Protein"},{"id":"T10295","span":{"begin":877,"end":891},"obj":"Protein"},{"id":"T10294","span":{"begin":990,"end":994},"obj":"Protein"},{"id":"T10293","span":{"begin":690,"end":709},"obj":"Entity"},{"id":"T10292","span":{"begin":607,"end":611},"obj":"Protein"},{"id":"T10291","span":{"begin":40,"end":46},"obj":"Entity"},{"id":"T10290","span":{"begin":664,"end":668},"obj":"Entity"},{"id":"T10289","span":{"begin":478,"end":559},"obj":"Entity"},{"id":"T10288","span":{"begin":333,"end":337},"obj":"Protein"},{"id":"T10287","span":{"begin":97,"end":116},"obj":"Protein"},{"id":"T10286","span":{"begin":212,"end":216},"obj":"Protein"},{"id":"T10285","span":{"begin":915,"end":971},"obj":"Entity"}],"relations":[{"id":"R7150","pred":"themeOf","subj":"T10292","obj":"T10303"},{"id":"R7151","pred":"themeOf","subj":"T10302","obj":"T10304"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T9851","span":{"begin":902,"end":911},"obj":"Gene_expression"},{"id":"T9850","span":{"begin":990,"end":994},"obj":"Protein"},{"id":"T9849","span":{"begin":882,"end":891},"obj":"Protein"},{"id":"T9848","span":{"begin":727,"end":737},"obj":"Gene_expression"},{"id":"T9847","span":{"begin":722,"end":726},"obj":"Protein"},{"id":"T9846","span":{"begin":622,"end":633},"obj":"Transcription"},{"id":"T9845","span":{"begin":607,"end":611},"obj":"Protein"},{"id":"T9844","span":{"begin":424,"end":428},"obj":"Protein"},{"id":"T9843","span":{"begin":311,"end":321},"obj":"Gene_expression"},{"id":"T9842","span":{"begin":333,"end":337},"obj":"Protein"},{"id":"T9841","span":{"begin":129,"end":136},"obj":"Gene_expression"},{"id":"T9840","span":{"begin":102,"end":111},"obj":"Negative_regulation"},{"id":"T9839","span":{"begin":212,"end":216},"obj":"Protein"},{"id":"T9838","span":{"begin":140,"end":146},"obj":"Protein"},{"id":"T9837","span":{"begin":97,"end":101},"obj":"Protein"},{"id":"T9836","span":{"begin":4,"end":14},"obj":"Gene_expression"},{"id":"T9835","span":{"begin":26,"end":30},"obj":"Protein"}],"relations":[{"id":"R6774","pred":"themeOf","subj":"T9835","obj":"T9836"},{"id":"R6775","pred":"themeOf","subj":"T9837","obj":"T9840"},{"id":"R6776","pred":"themeOf","subj":"T9838","obj":"T9841"},{"id":"R6777","pred":"themeOf","subj":"T9842","obj":"T9843"},{"id":"R6778","pred":"themeOf","subj":"T9845","obj":"T9846"},{"id":"R6779","pred":"themeOf","subj":"T9847","obj":"T9848"},{"id":"R6780","pred":"themeOf","subj":"T9849","obj":"T9851"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

testone

{"project":"testone","denotations":[{"id":"T9750","span":{"begin":902,"end":911},"obj":"Gene_expression"},{"id":"T9749","span":{"begin":727,"end":737},"obj":"Gene_expression"},{"id":"T9748","span":{"begin":129,"end":136},"obj":"Gene_expression"},{"id":"T9747","span":{"begin":102,"end":111},"obj":"Negative_regulation"},{"id":"T9746","span":{"begin":990,"end":994},"obj":"Protein"},{"id":"T9745","span":{"begin":882,"end":891},"obj":"Protein"},{"id":"T9744","span":{"begin":722,"end":726},"obj":"Protein"},{"id":"T9743","span":{"begin":607,"end":611},"obj":"Protein"},{"id":"T9742","span":{"begin":424,"end":428},"obj":"Protein"},{"id":"T9741","span":{"begin":333,"end":337},"obj":"Protein"},{"id":"T9740","span":{"begin":212,"end":216},"obj":"Protein"},{"id":"T9739","span":{"begin":137,"end":146},"obj":"Protein"},{"id":"T9738","span":{"begin":97,"end":101},"obj":"Protein"},{"id":"T9737","span":{"begin":26,"end":30},"obj":"Protein"}],"relations":[{"id":"R6760","pred":"themeOf","subj":"T9738","obj":"T9747"},{"id":"R6761","pred":"themeOf","subj":"T9739","obj":"T9748"},{"id":"R6762","pred":"themeOf","subj":"T9744","obj":"T9749"},{"id":"R6763","pred":"themeOf","subj":"T9745","obj":"T9750"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

test3

{"project":"test3","denotations":[{"id":"T9771","span":{"begin":990,"end":994},"obj":"Protein"},{"id":"T9770","span":{"begin":882,"end":891},"obj":"Protein"},{"id":"T9769","span":{"begin":727,"end":737},"obj":"Gene_expression"},{"id":"T9768","span":{"begin":722,"end":726},"obj":"Protein"},{"id":"T9767","span":{"begin":607,"end":611},"obj":"Protein"},{"id":"T9766","span":{"begin":424,"end":428},"obj":"Protein"},{"id":"T9765","span":{"begin":333,"end":337},"obj":"Protein"},{"id":"T9764","span":{"begin":212,"end":216},"obj":"Protein"},{"id":"T9763","span":{"begin":137,"end":146},"obj":"Protein"},{"id":"T9762","span":{"begin":97,"end":101},"obj":"Protein"},{"id":"T9761","span":{"begin":26,"end":30},"obj":"Protein"},{"id":"T9760","span":{"begin":990,"end":994},"obj":"Protein"},{"id":"T9759","span":{"begin":882,"end":891},"obj":"Protein"},{"id":"T9758","span":{"begin":722,"end":726},"obj":"Protein"},{"id":"T9757","span":{"begin":607,"end":611},"obj":"Protein"},{"id":"T9756","span":{"begin":424,"end":428},"obj":"Protein"},{"id":"T9755","span":{"begin":333,"end":337},"obj":"Protein"},{"id":"T9754","span":{"begin":212,"end":216},"obj":"Protein"},{"id":"T9753","span":{"begin":137,"end":146},"obj":"Protein"},{"id":"T9752","span":{"begin":97,"end":101},"obj":"Protein"},{"id":"T9751","span":{"begin":26,"end":30},"obj":"Protein"}],"relations":[{"id":"R6764","pred":"themeOf","subj":"T9768","obj":"T9769"}],"text":"The Expression Pattern of Sox6 Suggests a Role in Definitive Erythropoiesis\nThe observation that Sox6-deficient mice ectopically express ɛy globin in liver, where definitive erythroid cells mature, suggests that Sox6 is an important regulator in definitive erythropoiesis. To determine the temporal and spatial expression pattern of Sox6, Northern blot and in situ hybridization assays were employed. As shown in Figure 6A, Sox6 is detectable by Northern blot beginning at 10.5 dpc, coincident with the temporal onset of definitive erythropoiesis in the liver. Furthermore, in situ hybridization shows that Sox6 is highly transcribed in 12.5-dpc liver, but not in yolk sac blood islands at 7.5 dpc (Figure 6B). Therefore, Sox6 expression is temporally and spatially coincident with definitive, but not primitive, erythropoiesis. These data, taken together with the observation that ɛy globin is highly expressed in the liver cells of 14.5-dpc p100H mutant mice (Figure 5), demonstrate that Sox6 functions in definitive erythropoiesis.\nFigure 6 Expression Pattern of Sox6 by Northern Blot and In Situ Assays\n(A) Sox6 expression during embryonic development shown by Northern blot. Each lane contains 20 μg of total RNA from embryos whose ages are listed above each lane as dpc. The filter was hybridized with a 32P-labeled 575-bp mouse Sox6 cDNA fragment (nucleotides 1353–1927). Numbers on the left are sizes of standard marker fragments in kb.\n(B) Sox6 expression shown by in situ hybridization. Panel i: Sagittal section through an E12.5 mouse embryo using antisense Sox6. mRNA distribution is represented by pseudocolored red signal superimposed on the counterstained specimen. Sox6 transcripts are detected primarily in the fetal liver, developing nervous system, chondrocytes and craniofacial area. Panel ii: The sense control probe shows no signal above background. Panel iii: E7.5 embryo hybridized to antisense probe for Sox6. No signal is detected above background specifically in blood islands (or with the sense probe, unpublished data).\nThe size bars represent 100 μm."}

PMC:1359074 / 22433-24514 JSONTXT

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PMC:1359074 / 22433-24514 JSON TXT