PMC:1359074 / 19832-20658 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T142	0-20	Sentence	denotes	Figure 4 ChIP Assay
T143	21-120	Sentence	denotes	MEL cells (A) and 15.5-dpc fetal liver cells (B) were treated as detailed in Materials and Methods.
T144	121-342	Sentence	denotes	10% of the sample was saved as total input (Inp); remaining samples were divided: plus Sox6 antibody (Ab+), minus Sox6 antibody (Ab−), as well as no DNA (DNA−) and normal rabbit IgG (IgG) that served as negative controls.
T145	343-462	Sentence	denotes	Other controls for these experiments included PCR within the promoter of the α-globin gene and intron 24 of the p gene.
T146	463-501	Sentence	denotes	Both were negative (unpublished data).
T147	502-632	Sentence	denotes	PCR was carried out using primer pairs flanking the Sox/Sox6 binding sites (see Material and Methods) of the ɛy proximal promoter.
T148	633-722	Sentence	denotes	For all reactions, we used 2 μl of immuno-precipitated DNA and 2 μl of 1/100 total input.
T149	723-782	Sentence	denotes	Semiquantitative PCR was done within the exponential range.
T150	783-826	Sentence	denotes	Multiple independent experiments were done.