PMC:1359074 / 16334-16839 JSONTXT

{"project":"sentences","denotations":[{"id":"T112","span":{"begin":0,"end":79},"obj":"Sentence"},{"id":"T113","span":{"begin":80,"end":246},"obj":"Sentence"},{"id":"T114","span":{"begin":247,"end":335},"obj":"Sentence"},{"id":"T115","span":{"begin":336,"end":406},"obj":"Sentence"},{"id":"T116","span":{"begin":407,"end":505},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"(F) Both consensus Sox/Sox6 binding sites are required for Sox6 responsiveness. GM979 cells were transfected with a reporter construct (Figure 2A) containing −63 to +45 of the ɛy proximal promoter together with the CMV-Sox6 overexpression vector. Mutations of the consensus binding sites were also tested (M3, M2, M2 plus M3, see (A)). The fold repression of Sox6 with the WT or mutant constructs is shown. The baseline activities of the mutagenized reporter constructs are comparable to the WT construct."}