PMC:1315352 / 11903-12468 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/1315352","sourcedb":"PMC","sourceid":"1315352","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1315352","text":"Cloning and expression of Brachyury, DPPA5, CD9, E-Cadherin, GATA1, GATA6, Nanog, Oct3/4, PDX-1, PODXL, SOX2 and SOX17\nBrachyury (aa. 1–202), DPPA5 (a.a. 1–116), GATA1 (a.a. 1–413), GATA6 (aa. 1–449), Nanog (aa. 153–305), Oct3/4 (aa. 1–265), PDX-1 (aa. 1–283), SOX2 (aa. 135–317) and SOX17 (aa. 177–414) were expressed in E. Coli and extracellular domains of CD9, E-Cadherin, PODXL were expressed in mouse NSO cells. All proteins were purified and sequenced before they were used as antigens for immunizations and as substrate for antibody screening and subcloning.","divisions":[{"label":"Title","span":{"begin":0,"end":118}}],"tracks":[{"project":"CellFinder","denotations":[{"id":"T196","span":{"begin":182,"end":187},"obj":"GeneProtein"},{"id":"T197","span":{"begin":104,"end":108},"obj":"GeneProtein"},{"id":"T198","span":{"begin":113,"end":118},"obj":"GeneProtein"},{"id":"T199","span":{"begin":90,"end":95},"obj":"GeneProtein"},{"id":"T200","span":{"begin":82,"end":88},"obj":"GeneProtein"},{"id":"T201","span":{"begin":119,"end":128},"obj":"GeneProtein"},{"id":"T202","span":{"begin":68,"end":73},"obj":"GeneProtein"},{"id":"T203","span":{"begin":222,"end":228},"obj":"GeneProtein"},{"id":"T204","span":{"begin":201,"end":206},"obj":"GeneProtein"},{"id":"T205","span":{"begin":75,"end":80},"obj":"GeneProtein"},{"id":"T206","span":{"begin":376,"end":381},"obj":"GeneProtein"},{"id":"T207","span":{"begin":400,"end":405},"obj":"Species"},{"id":"T208","span":{"begin":364,"end":374},"obj":"GeneProtein"},{"id":"T209","span":{"begin":359,"end":362},"obj":"GeneProtein"},{"id":"T210","span":{"begin":284,"end":289},"obj":"GeneProtein"},{"id":"T211","span":{"begin":261,"end":265},"obj":"GeneProtein"},{"id":"T212","span":{"begin":242,"end":247},"obj":"GeneProtein"},{"id":"T213","span":{"begin":400,"end":415},"obj":"CellType"},{"id":"T214","span":{"begin":406,"end":415},"obj":"CellType"},{"id":"T215","span":{"begin":37,"end":42},"obj":"GeneProtein"},{"id":"T216","span":{"begin":44,"end":47},"obj":"GeneProtein"},{"id":"T217","span":{"begin":26,"end":35},"obj":"GeneProtein"},{"id":"T218","span":{"begin":97,"end":102},"obj":"GeneProtein"},{"id":"T219","span":{"begin":49,"end":59},"obj":"GeneProtein"},{"id":"T220","span":{"begin":61,"end":66},"obj":"GeneProtein"},{"id":"T221","span":{"begin":142,"end":147},"obj":"GeneProtein"},{"id":"T222","span":{"begin":162,"end":167},"obj":"GeneProtein"}],"attributes":[{"subj":"T196","pred":"source","obj":"CellFinder"},{"subj":"T197","pred":"source","obj":"CellFinder"},{"subj":"T198","pred":"source","obj":"CellFinder"},{"subj":"T199","pred":"source","obj":"CellFinder"},{"subj":"T200","pred":"source","obj":"CellFinder"},{"subj":"T201","pred":"source","obj":"CellFinder"},{"subj":"T202","pred":"source","obj":"CellFinder"},{"subj":"T203","pred":"source","obj":"CellFinder"},{"subj":"T204","pred":"source","obj":"CellFinder"},{"subj":"T205","pred":"source","obj":"CellFinder"},{"subj":"T206","pred":"source","obj":"CellFinder"},{"subj":"T207","pred":"source","obj":"CellFinder"},{"subj":"T208","pred":"source","obj":"CellFinder"},{"subj":"T209","pred":"source","obj":"CellFinder"},{"subj":"T210","pred":"source","obj":"CellFinder"},{"subj":"T211","pred":"source","obj":"CellFinder"},{"subj":"T212","pred":"source","obj":"CellFinder"},{"subj":"T213","pred":"source","obj":"CellFinder"},{"subj":"T214","pred":"source","obj":"CellFinder"},{"subj":"T215","pred":"source","obj":"CellFinder"},{"subj":"T216","pred":"source","obj":"CellFinder"},{"subj":"T217","pred":"source","obj":"CellFinder"},{"subj":"T218","pred":"source","obj":"CellFinder"},{"subj":"T219","pred":"source","obj":"CellFinder"},{"subj":"T220","pred":"source","obj":"CellFinder"},{"subj":"T221","pred":"source","obj":"CellFinder"},{"subj":"T222","pred":"source","obj":"CellFinder"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"CellFinder","color":"#a7ec93","default":true}]}]}}