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PMC:1315279 / 40147-41914 JSONTXT

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Id Subject Object Predicate Lexical cue
T9305 0-1 DT denotes A
T9306 18-23 NN denotes clone
T9307 2-3 NN denotes λ
T9308 4-17 NN denotes bacteriophage
T9309 60-68 VBN denotes isolated
T9310 24-36 VBG denotes encompassing
T9311 37-42 NN denotes Sam68
T9312 49-50 CD denotes 3
T9313 43-48 NNS denotes exons
T9314 51-53 IN denotes to
T9315 54-55 CD denotes 9
T9316 56-59 VBD denotes was
T9317 69-73 IN denotes from
T9318 74-75 DT denotes a
T9319 92-99 NN denotes library
T9320 76-79 CD denotes 129
T9321 80-83 NN denotes SvJ
T9322 79-80 HYPH denotes /
T9323 84-91 JJ denotes genomic
T9324 100-105 VBG denotes using
T9325 106-110 JJ denotes full
T9326 111-117 NN denotes length
T9327 110-111 HYPH denotes -
T9328 124-128 NN denotes cDNA
T9329 118-123 NN denotes Sam68
T9330 129-131 IN denotes as
T9331 132-133 DT denotes a
T9332 134-139 NN denotes probe
T9333 139-140 . denotes .
T9334 140-350 sentence denotes XbaI-digested Sam68 genomic DNA fragments of 4 kb (encompassing exon 4 and part of exon 5) and 3kb (spanning part of exon 5 and exon 6) were subcloned in Bluescript SK resulting in pBS4 and pBS3, respectively.
T9335 141-145 NN denotes XbaI
T9336 146-154 VBN denotes digested
T9337 145-146 HYPH denotes -
T9338 173-182 NNS denotes fragments
T9339 155-160 NN denotes Sam68
T9340 161-168 JJ denotes genomic
T9341 169-172 NN denotes DNA
T9342 282-291 VBN denotes subcloned
T9343 183-185 IN denotes of
T9344 186-187 CD denotes 4
T9345 188-190 NN denotes kb
T9346 191-192 -LRB- denotes (
T9347 192-204 VBG denotes encompassing
T9348 205-209 NN denotes exon
T9349 210-211 CD denotes 4
T9350 212-215 CC denotes and
T9351 216-220 NN denotes part
T9352 221-223 IN denotes of
T9353 224-228 NN denotes exon
T9354 229-230 CD denotes 5
T9355 230-231 -RRB- denotes )
T9356 232-235 CC denotes and
T9357 236-239 NN denotes 3kb
T9358 240-241 -LRB- denotes (
T9359 241-249 VBG denotes spanning
T9360 250-254 NN denotes part
T9361 255-257 IN denotes of
T9362 258-262 NN denotes exon
T9363 263-264 CD denotes 5
T9364 265-268 CC denotes and
T9365 269-273 NN denotes exon
T9366 274-275 CD denotes 6
T9367 275-276 -RRB- denotes )
T9368 277-281 VBD denotes were
T9369 292-294 IN denotes in
T9370 295-305 NN denotes Bluescript
T9371 306-308 NN denotes SK
T9372 309-318 VBG denotes resulting
T9373 319-321 IN denotes in
T9374 322-326 NN denotes pBS4
T9375 327-330 CC denotes and
T9376 331-335 NN denotes pBS3
T9377 335-337 , denotes ,
T9378 337-349 RB denotes respectively
T9379 349-350 . denotes .
T9380 350-497 sentence denotes A DNA fragment was amplified from pBS4 with the following oligonucleotides (5′-AAT GTC TAG AAA CAA CTC ATA TAC AGA C-3′) and the universal primer.
T9381 351-352 DT denotes A
T9382 357-365 NN denotes fragment
T9383 353-356 NN denotes DNA
T9384 370-379 VBN denotes amplified
T9385 366-369 VBD denotes was
T9386 380-384 IN denotes from
T9387 385-389 NN denotes pBS4
T9388 390-394 IN denotes with
T9389 395-398 DT denotes the
T9390 409-425 NNS denotes oligonucleotides
T9391 399-408 JJ denotes following
T9392 426-427 -LRB- denotes (
T9393 427-428 CD denotes 5
T9394 428-429 SYM denotes ′
T9395 429-430 HYPH denotes -
T9396 430-433 NN denotes AAT
T9397 466-467 NN denotes C
T9398 434-437 NN denotes GTC
T9399 438-441 NN denotes TAG
T9400 442-445 NN denotes AAA
T9401 446-449 NN denotes CAA
T9402 450-453 NN denotes CTC
T9403 454-457 NN denotes ATA
T9404 458-461 NN denotes TAC
T9405 462-465 NN denotes AGA
T9406 467-468 HYPH denotes -
T9407 468-469 CD denotes 3
T9408 469-470 SYM denotes ′
T9409 470-471 -RRB- denotes )
T9410 472-475 CC denotes and
T9411 476-479 DT denotes the
T9412 490-496 NN denotes primer
T9413 480-489 JJ denotes universal
T9414 496-497 . denotes .
T9415 497-648 sentence denotes The XbaI-digested 1-kb DNA fragment was subcloned in the XbaI site of pPNT (a gift from Andrew Karaplis, McGill University, Montréal, Quebec, Canada).
T9416 498-501 DT denotes The
T9417 525-533 NN denotes fragment
T9418 502-506 NN denotes XbaI
T9419 507-515 VBN denotes digested
T9420 506-507 HYPH denotes -
T9421 516-517 CD denotes 1
T9422 518-520 NN denotes kb
T9423 517-518 HYPH denotes -
T9424 521-524 NN denotes DNA
T9425 538-547 VBN denotes subcloned
T9426 534-537 VBD denotes was
T9427 548-550 IN denotes in
T9428 551-554 DT denotes the
T9429 560-564 NN denotes site
T9430 555-559 NN denotes XbaI
T9431 565-567 IN denotes of
T9432 568-572 NN denotes pPNT
T9433 573-574 -LRB- denotes (
T9434 576-580 NN denotes gift
T9435 574-575 DT denotes a
T9436 581-585 IN denotes from
T9437 586-592 NNP denotes Andrew
T9438 593-601 NNP denotes Karaplis
T9439 601-603 , denotes ,
T9440 603-609 NNP denotes McGill
T9441 610-620 NNP denotes University
T9442 620-622 , denotes ,
T9443 622-630 NNP denotes Montréal
T9444 630-632 , denotes ,
T9445 632-638 NNP denotes Quebec
T9446 638-640 , denotes ,
T9447 640-646 NNP denotes Canada
T9448 646-647 -RRB- denotes )
T9449 647-648 . denotes .
T9450 648-862 sentence denotes The 3-kb fragment from pBS3 was amplified by PCR with (5′-GGG ATG CGG CCG CTC TAG AAT TGT CCT ACT TGA ACG G-3') and (5′-CGG TGG CGG CCG CTG TCG ACC TGA GTA ACA TTT CTT A-3′) and subcloned in the NotI site of pPNT.
T9451 649-652 DT denotes The
T9452 658-666 NN denotes fragment
T9453 653-654 CD denotes 3
T9454 655-657 NN denotes kb
T9455 654-655 HYPH denotes -
T9456 681-690 VBN denotes amplified
T9457 667-671 IN denotes from
T9458 672-676 NN denotes pBS3
T9459 677-680 VBD denotes was
T9460 691-693 IN denotes by
T9461 694-697 NN denotes PCR
T9462 698-702 IN denotes with
T9463 703-704 -LRB- denotes (
T9464 704-705 CD denotes 5
T9465 705-706 SYM denotes ′
T9466 706-707 HYPH denotes -
T9467 707-710 NN denotes GGG
T9468 755-756 NN denotes G
T9469 711-714 NN denotes ATG
T9470 715-718 NN denotes CGG
T9471 719-722 NN denotes CCG
T9472 723-726 NN denotes CTC
T9473 727-730 NN denotes TAG
T9474 731-734 NN denotes AAT
T9475 735-738 NN denotes TGT
T9476 739-742 NN denotes CCT
T9477 743-746 NN denotes ACT
T9478 747-750 NN denotes TGA
T9479 751-754 NN denotes ACG
T9480 756-757 HYPH denotes -
T9481 757-758 CD denotes 3
T9482 758-759 SYM denotes '
T9483 759-760 -RRB- denotes )
T9484 761-764 CC denotes and
T9485 765-766 -LRB- denotes (
T9486 766-767 CD denotes 5
T9487 767-768 SYM denotes ′
T9488 768-769 HYPH denotes -
T9489 769-772 NN denotes CGG
T9490 817-818 NN denotes A
T9491 773-776 NN denotes TGG
T9492 777-780 NN denotes CGG
T9493 781-784 NN denotes CCG
T9494 785-788 NN denotes CTG
T9495 789-792 NN denotes TCG
T9496 793-796 NN denotes ACC
T9497 797-800 NN denotes TGA
T9498 801-804 NN denotes GTA
T9499 805-808 NN denotes ACA
T9500 809-812 NN denotes TTT
T9501 813-816 NN denotes CTT
T9502 818-819 HYPH denotes -
T9503 819-820 CD denotes 3
T9504 820-821 SYM denotes ′
T9505 821-822 -RRB- denotes )
T9506 823-826 CC denotes and
T9507 827-836 VBN denotes subcloned
T9508 837-839 IN denotes in
T9509 840-843 DT denotes the
T9510 849-853 NN denotes site
T9511 844-848 NN denotes NotI
T9512 854-856 IN denotes of
T9513 857-861 NN denotes pPNT
T9514 861-862 . denotes .
T9515 862-970 sentence denotes The targeting vector pPNT-Sam68 replaces exon 4 and part of exon 5 with a neomycin-resistant gene cassette.
T9516 863-866 DT denotes The
T9517 877-883 NN denotes vector
T9518 867-876 NN denotes targeting
T9519 895-903 VBZ denotes replaces
T9520 884-888 NN denotes pPNT
T9521 889-894 NN denotes Sam68
T9522 888-889 HYPH denotes -
T9523 904-908 NN denotes exon
T9524 909-910 CD denotes 4
T9525 911-914 CC denotes and
T9526 915-919 NN denotes part
T9527 920-922 IN denotes of
T9528 923-927 NN denotes exon
T9529 928-929 CD denotes 5
T9530 930-934 IN denotes with
T9531 935-936 DT denotes a
T9532 961-969 NN denotes cassette
T9533 937-945 NN denotes neomycin
T9534 946-955 JJ denotes resistant
T9535 945-946 HYPH denotes -
T9536 956-960 NN denotes gene
T9537 969-970 . denotes .
T9538 970-1127 sentence denotes An SalI site was introduced at the 3′ end of the 3-kb DNA fragment and was used to linearize the plasmid for electroporation into embryonic stem (ES) cells.
T9539 971-973 DT denotes An
T9540 979-983 NN denotes site
T9541 974-978 NN denotes SalI
T9542 988-998 VBN denotes introduced
T9543 984-987 VBD denotes was
T9544 999-1001 IN denotes at
T9545 1002-1005 DT denotes the
T9546 1009-1012 NN denotes end
T9547 1006-1007 CD denotes 3
T9548 1007-1008 SYM denotes ′
T9549 1013-1015 IN denotes of
T9550 1016-1019 DT denotes the
T9551 1029-1037 NN denotes fragment
T9552 1020-1021 CD denotes 3
T9553 1022-1024 NN denotes kb
T9554 1021-1022 HYPH denotes -
T9555 1025-1028 NN denotes DNA
T9556 1038-1041 CC denotes and
T9557 1042-1045 VBD denotes was
T9558 1046-1050 VBN denotes used
T9559 1051-1053 TO denotes to
T9560 1054-1063 VB denotes linearize
T9561 1064-1067 DT denotes the
T9562 1068-1075 NN denotes plasmid
T9563 1076-1079 IN denotes for
T9564 1080-1095 NN denotes electroporation
T9565 1096-1100 IN denotes into
T9566 1101-1110 JJ denotes embryonic
T9567 1111-1115 NN denotes stem
T9568 1121-1126 NNS denotes cells
T9569 1116-1117 -LRB- denotes (
T9570 1117-1119 NN denotes ES
T9571 1119-1120 -RRB- denotes )
T9572 1126-1127 . denotes .
T9573 1127-1280 sentence denotes Approximately 1,000 ES colonies were screened and two clones were identified that contained the Sam68 mutant allele, as determined by Southern blotting.
T9574 1128-1141 RB denotes Approximately
T9575 1142-1147 CD denotes 1,000
T9576 1151-1159 NNS denotes colonies
T9577 1148-1150 NN denotes ES
T9578 1165-1173 VBN denotes screened
T9579 1160-1164 VBD denotes were
T9580 1174-1177 CC denotes and
T9581 1178-1181 CD denotes two
T9582 1182-1188 NNS denotes clones
T9583 1194-1204 VBN denotes identified
T9584 1189-1193 VBD denotes were
T9585 1205-1209 WDT denotes that
T9586 1210-1219 VBD denotes contained
T9587 1220-1223 DT denotes the
T9588 1224-1229 NN denotes Sam68
T9589 1237-1243 NN denotes allele
T9590 1230-1236 NN denotes mutant
T9591 1243-1245 , denotes ,
T9592 1245-1247 IN denotes as
T9593 1248-1258 VBN denotes determined
T9594 1259-1261 IN denotes by
T9595 1262-1270 NNP denotes Southern
T9596 1271-1279 NN denotes blotting
T9597 1279-1280 . denotes .
T9598 1280-1473 sentence denotes Targeted ES cells were injected into 3.5-day-old BALB/c blastocysts and were transferred into CD-1 foster mothers, and animals classified as chimeras by coat color were mated with BALB/c mice.
T9599 1281-1289 VBN denotes Targeted
T9600 1293-1298 NNS denotes cells
T9601 1290-1292 NN denotes ES
T9602 1304-1312 VBN denotes injected
T9603 1299-1303 VBD denotes were
T9604 1313-1317 IN denotes into
T9605 1318-1321 CD denotes 3.5
T9606 1322-1325 NN denotes day
T9607 1321-1322 HYPH denotes -
T9608 1326-1329 JJ denotes old
T9609 1325-1326 HYPH denotes -
T9610 1337-1348 NNS denotes blastocysts
T9611 1330-1334 NN denotes BALB
T9612 1335-1336 NN denotes c
T9613 1334-1335 HYPH denotes /
T9614 1349-1352 CC denotes and
T9615 1353-1357 VBD denotes were
T9616 1358-1369 VBN denotes transferred
T9617 1370-1374 IN denotes into
T9618 1375-1377 NN denotes CD
T9619 1387-1394 NNS denotes mothers
T9620 1377-1378 HYPH denotes -
T9621 1378-1379 CD denotes 1
T9622 1380-1386 NN denotes foster
T9623 1394-1396 , denotes ,
T9624 1396-1399 CC denotes and
T9625 1400-1407 NNS denotes animals
T9626 1450-1455 VBN denotes mated
T9627 1408-1418 VBN denotes classified
T9628 1419-1421 IN denotes as
T9629 1422-1430 NNS denotes chimeras
T9630 1431-1433 IN denotes by
T9631 1434-1438 NN denotes coat
T9632 1439-1444 NN denotes color
T9633 1445-1449 VBD denotes were
T9634 1456-1460 IN denotes with
T9635 1461-1465 NN denotes BALB
T9636 1466-1467 NN denotes c
T9637 1465-1466 HYPH denotes /
T9638 1468-1472 NNS denotes mice
T9639 1472-1473 . denotes .
T9640 1473-1561 sentence denotes Germ line transmission was achieved and the mice were maintained in C57BL/6 background.
T9641 1474-1478 NN denotes Germ
T9642 1479-1483 NN denotes line
T9643 1484-1496 NN denotes transmission
T9644 1501-1509 VBN denotes achieved
T9645 1497-1500 VBD denotes was
T9646 1510-1513 CC denotes and
T9647 1514-1517 DT denotes the
T9648 1518-1522 NNS denotes mice
T9649 1528-1538 VBN denotes maintained
T9650 1523-1527 VBD denotes were
T9651 1539-1541 IN denotes in
T9652 1542-1547 NN denotes C57BL
T9653 1550-1560 NN denotes background
T9654 1547-1548 HYPH denotes /
T9655 1548-1549 CD denotes 6
T9656 1560-1561 . denotes .
T9657 1561-1767 sentence denotes The mice used for this study represent mice that were backcrossed in C57BL/6 between three and eight generations; in addition, we maintained the mice in the 129/SvJ strain and observed a similar phenotype.
T9658 1562-1565 DT denotes The
T9659 1566-1570 NNS denotes mice
T9660 1591-1600 VBP denotes represent
T9661 1571-1575 VBN denotes used
T9662 1576-1579 IN denotes for
T9663 1580-1584 DT denotes this
T9664 1585-1590 NN denotes study
T9665 1692-1702 VBD denotes maintained
T9666 1601-1605 NNS denotes mice
T9667 1606-1610 WDT denotes that
T9668 1616-1627 VBN denotes backcrossed
T9669 1611-1615 VBD denotes were
T9670 1628-1630 IN denotes in
T9671 1631-1636 NN denotes C57BL
T9672 1636-1637 HYPH denotes /
T9673 1637-1638 CD denotes 6
T9674 1639-1646 IN denotes between
T9675 1647-1652 CD denotes three
T9676 1663-1674 NNS denotes generations
T9677 1653-1656 CC denotes and
T9678 1657-1662 CD denotes eight
T9679 1674-1675 : denotes ;
T9680 1676-1678 IN denotes in
T9681 1679-1687 NN denotes addition
T9682 1687-1689 , denotes ,
T9683 1689-1691 PRP denotes we
T9684 1703-1706 DT denotes the
T9685 1707-1711 NNS denotes mice
T9686 1712-1714 IN denotes in
T9687 1715-1718 DT denotes the
T9688 1727-1733 NN denotes strain
T9689 1719-1722 CD denotes 129
T9690 1723-1726 NN denotes SvJ
T9691 1722-1723 HYPH denotes /
T9692 1734-1737 CC denotes and
T9693 1738-1746 VBD denotes observed
T9694 1747-1748 DT denotes a
T9695 1757-1766 NN denotes phenotype
T9696 1749-1756 JJ denotes similar
T9697 1766-1767 . denotes .
R5782 T9305 T9306 det A,clone
R5783 T9306 T9309 nsubjpass clone,isolated
R5784 T9307 T9306 compound λ,clone
R5785 T9308 T9306 compound bacteriophage,clone
R5786 T9310 T9306 acl encompassing,clone
R5787 T9311 T9312 nmod Sam68,3
R5788 T9312 T9310 dobj 3,encompassing
R5789 T9313 T9312 nmod exons,3
R5790 T9314 T9312 prep to,3
R5791 T9315 T9314 pobj 9,to
R5792 T9316 T9309 auxpass was,isolated
R5793 T9317 T9309 prep from,isolated
R5794 T9318 T9319 det a,library
R5795 T9319 T9317 pobj library,from
R5796 T9320 T9321 nummod 129,SvJ
R5797 T9321 T9319 nmod SvJ,library
R5798 T9322 T9321 punct /,SvJ
R5799 T9323 T9319 amod genomic,library
R5800 T9324 T9309 advcl using,isolated
R5801 T9325 T9326 amod full,length
R5802 T9326 T9328 compound length,cDNA
R5803 T9327 T9326 punct -,length
R5804 T9328 T9324 dobj cDNA,using
R5805 T9329 T9328 compound Sam68,cDNA
R5806 T9330 T9324 prep as,using
R5807 T9331 T9332 det a,probe
R5808 T9332 T9330 pobj probe,as
R5809 T9333 T9309 punct .,isolated
R5810 T9335 T9336 npadvmod XbaI,digested
R5811 T9336 T9338 amod digested,fragments
R5812 T9337 T9336 punct -,digested
R5813 T9338 T9342 nsubjpass fragments,subcloned
R5814 T9339 T9338 nmod Sam68,fragments
R5815 T9340 T9338 amod genomic,fragments
R5816 T9341 T9338 compound DNA,fragments
R5817 T9343 T9338 prep of,fragments
R5818 T9344 T9345 nummod 4,kb
R5819 T9345 T9343 pobj kb,of
R5820 T9346 T9347 punct (,encompassing
R5821 T9347 T9345 parataxis encompassing,kb
R5822 T9348 T9347 dobj exon,encompassing
R5823 T9349 T9348 nummod 4,exon
R5824 T9350 T9348 cc and,exon
R5825 T9351 T9348 conj part,exon
R5826 T9352 T9351 prep of,part
R5827 T9353 T9352 pobj exon,of
R5828 T9354 T9353 nummod 5,exon
R5829 T9355 T9347 punct ),encompassing
R5830 T9356 T9345 cc and,kb
R5831 T9357 T9345 conj 3kb,kb
R5832 T9358 T9359 punct (,spanning
R5833 T9359 T9357 parataxis spanning,3kb
R5834 T9360 T9359 dobj part,spanning
R5835 T9361 T9360 prep of,part
R5836 T9362 T9361 pobj exon,of
R5837 T9363 T9362 nummod 5,exon
R5838 T9364 T9360 cc and,part
R5839 T9365 T9360 conj exon,part
R5840 T9366 T9365 nummod 6,exon
R5841 T9367 T9359 punct ),spanning
R5842 T9368 T9342 auxpass were,subcloned
R5843 T9369 T9342 prep in,subcloned
R5844 T9370 T9371 compound Bluescript,SK
R5845 T9371 T9369 pobj SK,in
R5846 T9372 T9342 advcl resulting,subcloned
R5847 T9373 T9372 prep in,resulting
R5848 T9374 T9373 pobj pBS4,in
R5849 T9375 T9374 cc and,pBS4
R5850 T9376 T9374 conj pBS3,pBS4
R5851 T9377 T9372 punct ", ",resulting
R5852 T9378 T9372 advmod respectively,resulting
R5853 T9379 T9342 punct .,subcloned
R5854 T9381 T9382 det A,fragment
R5855 T9382 T9384 nsubjpass fragment,amplified
R5856 T9383 T9382 compound DNA,fragment
R5857 T9385 T9384 auxpass was,amplified
R5858 T9386 T9384 prep from,amplified
R5859 T9387 T9386 pobj pBS4,from
R5860 T9388 T9384 prep with,amplified
R5861 T9389 T9390 det the,oligonucleotides
R5862 T9390 T9388 pobj oligonucleotides,with
R5863 T9391 T9390 amod following,oligonucleotides
R5864 T9392 T9393 punct (,5
R5865 T9393 T9390 parataxis 5,oligonucleotides
R5866 T9394 T9393 punct ′,5
R5867 T9395 T9393 punct -,5
R5868 T9396 T9397 compound AAT,C
R5869 T9397 T9393 appos C,5
R5870 T9398 T9397 compound GTC,C
R5871 T9399 T9397 compound TAG,C
R5872 T9400 T9397 compound AAA,C
R5873 T9401 T9397 compound CAA,C
R5874 T9402 T9397 compound CTC,C
R5875 T9403 T9397 compound ATA,C
R5876 T9404 T9397 compound TAC,C
R5877 T9405 T9397 compound AGA,C
R5878 T9406 T9393 punct -,5
R5879 T9407 T9393 nummod 3,5
R5880 T9408 T9393 punct ′,5
R5881 T9409 T9393 punct ),5
R5882 T9410 T9390 cc and,oligonucleotides
R5883 T9411 T9412 det the,primer
R5884 T9412 T9390 conj primer,oligonucleotides
R5885 T9413 T9412 amod universal,primer
R5886 T9414 T9384 punct .,amplified
R5887 T9416 T9417 det The,fragment
R5888 T9417 T9425 nsubjpass fragment,subcloned
R5889 T9418 T9419 npadvmod XbaI,digested
R5890 T9419 T9417 amod digested,fragment
R5891 T9420 T9419 punct -,digested
R5892 T9421 T9422 nummod 1,kb
R5893 T9422 T9417 compound kb,fragment
R5894 T9423 T9422 punct -,kb
R5895 T9424 T9417 compound DNA,fragment
R5896 T9426 T9425 auxpass was,subcloned
R5897 T9427 T9425 prep in,subcloned
R5898 T9428 T9429 det the,site
R5899 T9429 T9427 pobj site,in
R5900 T9430 T9429 compound XbaI,site
R5901 T9431 T9429 prep of,site
R5902 T9432 T9431 pobj pPNT,of
R5903 T9433 T9434 punct (,gift
R5904 T9434 T9432 parataxis gift,pPNT
R5905 T9435 T9434 det a,gift
R5906 T9436 T9434 prep from,gift
R5907 T9437 T9438 compound Andrew,Karaplis
R5908 T9438 T9436 pobj Karaplis,from
R5909 T9439 T9438 punct ", ",Karaplis
R5910 T9440 T9441 compound McGill,University
R5911 T9441 T9438 npadvmod University,Karaplis
R5912 T9442 T9438 punct ", ",Karaplis
R5913 T9443 T9438 npadvmod Montréal,Karaplis
R5914 T9444 T9438 punct ", ",Karaplis
R5915 T9445 T9438 npadvmod Quebec,Karaplis
R5916 T9446 T9438 punct ", ",Karaplis
R5917 T9447 T9438 npadvmod Canada,Karaplis
R5918 T9448 T9434 punct ),gift
R5919 T9449 T9425 punct .,subcloned
R5920 T9451 T9452 det The,fragment
R5921 T9452 T9456 nsubjpass fragment,amplified
R5922 T9453 T9454 nummod 3,kb
R5923 T9454 T9452 compound kb,fragment
R5924 T9455 T9454 punct -,kb
R5925 T9457 T9452 prep from,fragment
R5926 T9458 T9457 pobj pBS3,from
R5927 T9459 T9456 auxpass was,amplified
R5928 T9460 T9456 prep by,amplified
R5929 T9461 T9460 pobj PCR,by
R5930 T9462 T9461 prep with,PCR
R5931 T9463 T9462 punct (,with
R5932 T9464 T9462 pobj 5,with
R5933 T9465 T9464 punct ′,5
R5934 T9466 T9464 punct -,5
R5935 T9467 T9468 compound GGG,G
R5936 T9468 T9464 appos G,5
R5937 T9469 T9468 compound ATG,G
R5938 T9470 T9468 compound CGG,G
R5939 T9471 T9468 compound CCG,G
R5940 T9472 T9468 compound CTC,G
R5941 T9473 T9468 compound TAG,G
R5942 T9474 T9468 compound AAT,G
R5943 T9475 T9468 compound TGT,G
R5944 T9476 T9468 compound CCT,G
R5945 T9477 T9468 compound ACT,G
R5946 T9478 T9468 compound TGA,G
R5947 T9479 T9468 compound ACG,G
R5948 T9480 T9464 punct -,5
R5949 T9481 T9464 nummod 3,5
R5950 T9482 T9464 punct ',5
R5951 T9483 T9464 punct ),5
R5952 T9484 T9464 cc and,5
R5953 T9485 T9486 punct (,5
R5954 T9486 T9464 conj 5,5
R5955 T9487 T9486 punct ′,5
R5956 T9488 T9486 punct -,5
R5957 T9489 T9490 compound CGG,A
R5958 T9490 T9486 appos A,5
R5959 T9491 T9490 compound TGG,A
R5960 T9492 T9490 compound CGG,A
R5961 T9493 T9490 compound CCG,A
R5962 T9494 T9490 compound CTG,A
R5963 T9495 T9490 compound TCG,A
R5964 T9496 T9490 compound ACC,A
R5965 T9497 T9490 compound TGA,A
R5966 T9498 T9490 compound GTA,A
R5967 T9499 T9490 compound ACA,A
R5968 T9500 T9490 compound TTT,A
R5969 T9501 T9490 compound CTT,A
R5970 T9502 T9486 punct -,5
R5971 T9503 T9486 nummod 3,5
R5972 T9504 T9486 punct ′,5
R5973 T9505 T9456 punct ),amplified
R5974 T9506 T9456 cc and,amplified
R5975 T9507 T9456 conj subcloned,amplified
R5976 T9508 T9507 prep in,subcloned
R5977 T9509 T9510 det the,site
R5978 T9510 T9508 pobj site,in
R5979 T9511 T9510 compound NotI,site
R5980 T9512 T9510 prep of,site
R5981 T9513 T9512 pobj pPNT,of
R5982 T9514 T9456 punct .,amplified
R5983 T9516 T9517 det The,vector
R5984 T9517 T9519 nsubj vector,replaces
R5985 T9518 T9517 compound targeting,vector
R5986 T9520 T9521 compound pPNT,Sam68
R5987 T9521 T9517 appos Sam68,vector
R5988 T9522 T9521 punct -,Sam68
R5989 T9523 T9519 dobj exon,replaces
R5990 T9524 T9523 nummod 4,exon
R5991 T9525 T9523 cc and,exon
R5992 T9526 T9523 conj part,exon
R5993 T9527 T9526 prep of,part
R5994 T9528 T9527 pobj exon,of
R5995 T9529 T9528 nummod 5,exon
R5996 T9530 T9519 prep with,replaces
R5997 T9531 T9532 det a,cassette
R5998 T9532 T9530 pobj cassette,with
R5999 T9533 T9534 npadvmod neomycin,resistant
R6000 T9534 T9532 amod resistant,cassette
R6001 T9535 T9534 punct -,resistant
R6002 T9536 T9532 compound gene,cassette
R6003 T9537 T9519 punct .,replaces
R6004 T9539 T9540 det An,site
R6005 T9540 T9542 nsubjpass site,introduced
R6006 T9541 T9540 compound SalI,site
R6007 T9543 T9542 auxpass was,introduced
R6008 T9544 T9542 prep at,introduced
R6009 T9545 T9546 det the,end
R6010 T9546 T9544 pobj end,at
R6011 T9547 T9546 nummod 3,end
R6012 T9548 T9547 punct ′,3
R6013 T9549 T9546 prep of,end
R6014 T9550 T9551 det the,fragment
R6015 T9551 T9549 pobj fragment,of
R6016 T9552 T9553 nummod 3,kb
R6017 T9553 T9551 compound kb,fragment
R6018 T9554 T9553 punct -,kb
R6019 T9555 T9551 compound DNA,fragment
R6020 T9556 T9542 cc and,introduced
R6021 T9557 T9558 auxpass was,used
R6022 T9558 T9542 conj used,introduced
R6023 T9559 T9560 aux to,linearize
R6024 T9560 T9558 advcl linearize,used
R6025 T9561 T9562 det the,plasmid
R6026 T9562 T9560 dobj plasmid,linearize
R6027 T9563 T9560 prep for,linearize
R6028 T9564 T9563 pobj electroporation,for
R6029 T9565 T9564 prep into,electroporation
R6030 T9566 T9567 amod embryonic,stem
R6031 T9567 T9568 nmod stem,cells
R6032 T9568 T9565 pobj cells,into
R6033 T9569 T9567 punct (,stem
R6034 T9570 T9567 appos ES,stem
R6035 T9571 T9568 punct ),cells
R6036 T9572 T9542 punct .,introduced
R6037 T9574 T9575 advmod Approximately,"1,000"
R6038 T9575 T9576 nummod "1,000",colonies
R6039 T9576 T9578 nsubjpass colonies,screened
R6040 T9577 T9576 compound ES,colonies
R6041 T9579 T9578 auxpass were,screened
R6042 T9580 T9578 cc and,screened
R6043 T9581 T9582 nummod two,clones
R6044 T9582 T9583 nsubjpass clones,identified
R6045 T9583 T9578 conj identified,screened
R6046 T9584 T9583 auxpass were,identified
R6047 T9585 T9586 dep that,contained
R6048 T9586 T9583 ccomp contained,identified
R6049 T9587 T9588 det the,Sam68
R6050 T9588 T9589 compound Sam68,allele
R6051 T9589 T9586 dobj allele,contained
R6052 T9590 T9589 compound mutant,allele
R6053 T9591 T9583 punct ", ",identified
R6054 T9592 T9593 mark as,determined
R6055 T9593 T9583 advcl determined,identified
R6056 T9594 T9593 prep by,determined
R6057 T9595 T9596 compound Southern,blotting
R6058 T9596 T9594 pobj blotting,by
R6059 T9597 T9583 punct .,identified
R6060 T9599 T9600 amod Targeted,cells
R6061 T9600 T9602 nsubjpass cells,injected
R6062 T9601 T9600 compound ES,cells
R6063 T9603 T9602 auxpass were,injected
R6064 T9604 T9602 prep into,injected
R6065 T9605 T9606 nummod 3.5,day
R6066 T9606 T9608 npadvmod day,old
R6067 T9607 T9606 punct -,day
R6068 T9608 T9610 amod old,blastocysts
R6069 T9609 T9608 punct -,old
R6070 T9610 T9604 pobj blastocysts,into
R6071 T9611 T9612 compound BALB,c
R6072 T9612 T9610 compound c,blastocysts
R6073 T9613 T9612 punct /,c
R6074 T9614 T9602 cc and,injected
R6075 T9615 T9616 auxpass were,transferred
R6076 T9616 T9602 conj transferred,injected
R6077 T9617 T9616 prep into,transferred
R6078 T9618 T9619 nmod CD,mothers
R6079 T9619 T9617 pobj mothers,into
R6080 T9620 T9618 punct -,CD
R6081 T9621 T9618 nummod 1,CD
R6082 T9622 T9619 compound foster,mothers
R6083 T9623 T9602 punct ", ",injected
R6084 T9624 T9602 cc and,injected
R6085 T9625 T9626 nsubjpass animals,mated
R6086 T9626 T9602 conj mated,injected
R6087 T9627 T9625 acl classified,animals
R6088 T9628 T9627 prep as,classified
R6089 T9629 T9628 pobj chimeras,as
R6090 T9630 T9627 prep by,classified
R6091 T9631 T9632 compound coat,color
R6092 T9632 T9630 pobj color,by
R6093 T9633 T9626 auxpass were,mated
R6094 T9634 T9626 prep with,mated
R6095 T9635 T9636 compound BALB,c
R6096 T9636 T9638 compound c,mice
R6097 T9637 T9636 punct /,c
R6098 T9638 T9634 pobj mice,with
R6099 T9639 T9626 punct .,mated
R6100 T9641 T9642 compound Germ,line
R6101 T9642 T9643 compound line,transmission
R6102 T9643 T9644 nsubjpass transmission,achieved
R6103 T9645 T9644 auxpass was,achieved
R6104 T9646 T9644 cc and,achieved
R6105 T9647 T9648 det the,mice
R6106 T9648 T9649 nsubjpass mice,maintained
R6107 T9649 T9644 conj maintained,achieved
R6108 T9650 T9649 auxpass were,maintained
R6109 T9651 T9649 prep in,maintained
R6110 T9652 T9653 nmod C57BL,background
R6111 T9653 T9651 pobj background,in
R6112 T9654 T9652 punct /,C57BL
R6113 T9655 T9652 nummod 6,C57BL
R6114 T9656 T9649 punct .,maintained
R6115 T9658 T9659 det The,mice
R6116 T9659 T9660 nsubj mice,represent
R6117 T9660 T9665 ccomp represent,maintained
R6118 T9661 T9659 acl used,mice
R6119 T9662 T9661 prep for,used
R6120 T9663 T9664 det this,study
R6121 T9664 T9662 pobj study,for
R6122 T9666 T9660 dobj mice,represent
R6123 T9667 T9668 dep that,backcrossed
R6124 T9668 T9666 relcl backcrossed,mice
R6125 T9669 T9668 auxpass were,backcrossed
R6126 T9670 T9668 prep in,backcrossed
R6127 T9671 T9670 pobj C57BL,in
R6128 T9672 T9671 punct /,C57BL
R6129 T9673 T9671 nummod 6,C57BL
R6130 T9674 T9675 quantmod between,three
R6131 T9675 T9676 nummod three,generations
R6132 T9676 T9668 npadvmod generations,backcrossed
R6133 T9677 T9675 cc and,three
R6134 T9678 T9675 conj eight,three
R6135 T9679 T9665 punct ;,maintained
R6136 T9680 T9665 prep in,maintained
R6137 T9681 T9680 pobj addition,in
R6138 T9682 T9665 punct ", ",maintained
R6139 T9683 T9665 nsubj we,maintained
R6140 T9684 T9685 det the,mice
R6141 T9685 T9665 dobj mice,maintained
R6142 T9686 T9685 prep in,mice
R6143 T9687 T9688 det the,strain
R6144 T9688 T9686 pobj strain,in
R6145 T9689 T9690 nummod 129,SvJ
R6146 T9690 T9688 compound SvJ,strain
R6147 T9691 T9690 punct /,SvJ
R6148 T9692 T9665 cc and,maintained
R6149 T9693 T9665 conj observed,maintained
R6150 T9694 T9695 det a,phenotype
R6151 T9695 T9693 dobj phenotype,observed
R6152 T9696 T9695 amod similar,phenotype
R6153 T9697 T9665 punct .,maintained

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T9201 37-42 PR:000009279 denotes Sam68
T9202 43-48 SO:0000147 denotes exons
T9203 84-91 SO:0001026 denotes genomic
T9204 118-123 PR:000009279 denotes Sam68
T9205 155-160 PR:000009279 denotes Sam68
T9206 161-168 SO:0001026 denotes genomic
T9207 205-209 SO:0000147 denotes exon
T9208 216-228 SO:0000852 denotes part of exon
T9209 250-262 SO:0000852 denotes part of exon
T9210 269-273 SO:0000147 denotes exon
T9211 490-496 SO:0000112 denotes primer
T9212 867-883 SO:0001644 denotes targeting vector
T9213 889-894 PR:000009279 denotes Sam68
T9214 904-908 SO:0000147 denotes exon
T9215 915-927 SO:0000852 denotes part of exon
T9216 937-945 CHEBI:7507 denotes neomycin
T9217 956-969 SO:0005853 denotes gene cassette
T9218 1068-1075 SO:0000155 denotes plasmid
T9219 1101-1110 UBERON:0000922 denotes embryonic
T9220 1101-1115 _FRAGMENT denotes embryonic stem
T9221 1121-1126 CL:0002322 denotes cells
T9222 1117-1119 _FRAGMENT denotes ES
T9223 1148-1150 CL:0002322 denotes ES
T9224 1224-1229 PR:000009279 denotes Sam68
T9225 1237-1243 SO:0001023 denotes allele
T9226 1290-1298 CL:0002322 denotes ES cells
T9227 1337-1348 UBERON:0000358 denotes blastocysts
T9228 1400-1407 NCBITaxon:33208 denotes animals
T9229 1434-1438 UBERON:0010166 denotes coat
T9230 1450-1455 GO:0007618 denotes mated
T9231 1468-1472 NCBITaxon:10088 denotes mice
T9232 1518-1522 NCBITaxon:10088 denotes mice
T9233 1566-1570 NCBITaxon:10088 denotes mice
T9234 1601-1605 NCBITaxon:10088 denotes mice
T9235 1707-1711 NCBITaxon:10088 denotes mice
R5768 T9221 T9220 _lexicallyChainedTo cells,embryonic stem
R5769 T9221 T9222 _lexicallyChainedTo cells,ES

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T9239 18-23 SO_EXT:sequence_cloned_entity denotes clone
T9240 37-42 PR_EXT:000009279 denotes Sam68
T9241 43-48 SO_EXT:0000147 denotes exons
T9242 84-91 SO_EXT:0001026 denotes genomic
T9243 118-123 PR_EXT:000009279 denotes Sam68
T9244 124-128 SO_EXT:cDNA denotes cDNA
T9245 134-139 CHEBI_SO_EXT:molecular_probe denotes probe
T9246 155-160 PR_EXT:000009279 denotes Sam68
T9247 161-172 SO_EXT:genomic_DNA denotes genomic DNA
T9248 169-172 CHEBI_SO_EXT:DNA denotes DNA
T9249 189-190 CHEBI_SO_EXT:base denotes b
T9250 205-209 SO_EXT:0000147 denotes exon
T9251 216-228 SO_EXT:0000852 denotes part of exon
T9252 250-262 SO_EXT:0000852 denotes part of exon
T9253 269-273 SO_EXT:0000147 denotes exon
T9254 353-356 CHEBI_SO_EXT:DNA denotes DNA
T9255 409-425 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T9256 490-496 SO_EXT:0000112 denotes primer
T9257 519-520 CHEBI_SO_EXT:base denotes b
T9258 521-524 CHEBI_SO_EXT:DNA denotes DNA
T9259 656-657 CHEBI_SO_EXT:base denotes b
T9260 867-883 SO_EXT:0001644 denotes targeting vector
T9261 889-894 PR_EXT:000009279 denotes Sam68
T9262 895-903 SO_EXT:sequence_substitution_process denotes replaces
T9263 904-908 SO_EXT:0000147 denotes exon
T9264 915-927 SO_EXT:0000852 denotes part of exon
T9265 937-945 CHEBI:7507 denotes neomycin
T9266 956-969 SO_EXT:0005853 denotes gene cassette
T9267 1023-1024 CHEBI_SO_EXT:base denotes b
T9268 1025-1028 CHEBI_SO_EXT:DNA denotes DNA
T9269 1054-1063 SO_EXT:sequence_linearization denotes linearize
T9270 1068-1075 SO_EXT:0000155 denotes plasmid
T9271 1101-1110 UBERON:0000922 denotes embryonic
T9272 1101-1115 _FRAGMENT denotes embryonic stem
T9273 1121-1126 CL:0002322 denotes cells
T9274 1117-1119 _FRAGMENT denotes ES
T9275 1121-1126 CL_GO_EXT:cell denotes cells
T9276 1148-1150 CL:0002322 denotes ES
T9277 1182-1188 SO_EXT:sequence_cloned_entity denotes clones
T9278 1224-1229 PR_EXT:000009279 denotes Sam68
T9279 1230-1236 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T9280 1237-1243 SO_EXT:0001023 denotes allele
T9281 1290-1298 CL:0002322 denotes ES cells
T9282 1293-1298 CL_GO_EXT:cell denotes cells
T9283 1337-1348 UBERON:0000358 denotes blastocysts
T9284 1358-1369 GO_EXT:biological_movement_or_translocation_process denotes transferred
T9285 1400-1407 NCBITaxon:33208 denotes animals
T9286 1434-1438 UBERON:0010166 denotes coat
T9287 1450-1455 GO:0007618 denotes mated
T9288 1468-1472 NCBITaxon:10088 denotes mice
T9289 1518-1522 NCBITaxon:10088 denotes mice
T9290 1566-1570 NCBITaxon:10088 denotes mice
T9291 1601-1605 NCBITaxon:10088 denotes mice
T9292 1707-1711 NCBITaxon:10088 denotes mice
R5770 T9273 T9272 _lexicallyChainedTo cells,embryonic stem
R5771 T9273 T9274 _lexicallyChainedTo cells,ES