PMC:1315279 / 23319-24385
Annnotations
craft-sa-dev
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mice stained equally well for TRAP and excavated approximately equal numbers of pits of equal size in dentin slices, as visualized by scanning electron microscopy (Figure 6B). These findings suggest that the osteoclasts may not be the primary defect in Sam68−/− mice, as they are in Src null mice [48].\nFigure 6 Ex Vivo Activity of Sam68+/+ and Sam68−/− Osteoblasts and Osteoclasts\nMarrow stromal cells were isolated from the long bones of juvenile mice and maintained under conditions that promote osteoblast differentiation.\n(A) Cultures were fixed in 4% paraformaldehyde after 6 or 18 days and stained in situ for ALP activity and with silver nitrate (von Kossa) to detect mineralized nodules. Sam68−/− cultures stained more intensely for ALP at early and late time points and produced significantly more mineralized nodules after 18 days. Asterisks represent p \u003c 0.01.\n(B) Primary osteoclasts were isolated from the crushed long bones of the same mice and plated on glass coverslips or on dentin slices to quantify numbers and activity, respectively. Osteoclas"}
2_test
{"project":"2_test","denotations":[{"id":"16362077-1997203-85614462","span":{"begin":299,"end":301},"obj":"1997203"},{"id":"T17644","span":{"begin":299,"end":301},"obj":"1997203"}],"text":" mice stained equally well for TRAP and excavated approximately equal numbers of pits of equal size in dentin slices, as visualized by scanning electron microscopy (Figure 6B). These findings suggest that the osteoclasts may not be the primary defect in Sam68−/− mice, as they are in Src null mice [48].\nFigure 6 Ex Vivo Activity of Sam68+/+ and Sam68−/− Osteoblasts and Osteoclasts\nMarrow stromal cells were isolated from the long bones of juvenile mice and maintained under conditions that promote osteoblast differentiation.\n(A) Cultures were fixed in 4% paraformaldehyde after 6 or 18 days and stained in situ for ALP activity and with silver nitrate (von Kossa) to detect mineralized nodules. Sam68−/− cultures stained more intensely for ALP at early and late time points and produced significantly more mineralized nodules after 18 days. Asterisks represent p \u003c 0.01.\n(B) Primary osteoclasts were isolated from the crushed long bones of the same mice and plated on glass coverslips or on dentin slices to quantify numbers and activity, respectively. Osteoclas"}
craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T4869","span":{"begin":1,"end":5},"obj":"NCBITaxon:10088"},{"id":"T4870","span":{"begin":31,"end":35},"obj":"PR:000001937"},{"id":"T4871","span":{"begin":103,"end":109},"obj":"UBERON:0001751"},{"id":"T4872","span":{"begin":144,"end":152},"obj":"CHEBI:10545"},{"id":"T4873","span":{"begin":209,"end":220},"obj":"CL:0000092"},{"id":"T4874","span":{"begin":254,"end":259},"obj":"PR:000009279"},{"id":"T4875","span":{"begin":263,"end":267},"obj":"NCBITaxon:10088"},{"id":"T4876","span":{"begin":284,"end":287},"obj":"PR:000027234"},{"id":"T4877","span":{"begin":293,"end":297},"obj":"NCBITaxon:10088"}],"text":" mice stained equally well for TRAP and excavated approximately equal numbers of pits of equal size in dentin slices, as visualized by scanning electron microscopy (Figure 6B). These findings suggest that the osteoclasts may not be the primary defect in Sam68−/− mice, as they are in Src null mice [48].\nFigure 6 Ex Vivo Activity of Sam68+/+ and Sam68−/− Osteoblasts and Osteoclasts\nMarrow stromal cells were isolated from the long bones of juvenile mice and maintained under conditions that promote osteoblast differentiation.\n(A) Cultures were fixed in 4% paraformaldehyde after 6 or 18 days and stained in situ for ALP activity and with silver nitrate (von Kossa) to detect mineralized nodules. Sam68−/− cultures stained more intensely for ALP at early and late time points and produced significantly more mineralized nodules after 18 days. Asterisks represent p \u003c 0.01.\n(B) Primary osteoclasts were isolated from the crushed long bones of the same mice and plated on glass coverslips or on dentin slices to quantify numbers and activity, respectively. Osteoclas"}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T4944","span":{"begin":1,"end":5},"obj":"NCBITaxon:10088"},{"id":"T4945","span":{"begin":31,"end":35},"obj":"PR_EXT:000001937"},{"id":"T4946","span":{"begin":103,"end":109},"obj":"UBERON:0001751"},{"id":"T4947","span":{"begin":144,"end":152},"obj":"CHEBI:10545"},{"id":"T4948","span":{"begin":209,"end":220},"obj":"CL:0000092"},{"id":"T4949","span":{"begin":254,"end":259},"obj":"PR_EXT:000009279"},{"id":"T4950","span":{"begin":259,"end":260},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4951","span":{"begin":261,"end":262},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4952","span":{"begin":263,"end":267},"obj":"NCBITaxon:10088"},{"id":"T4953","span":{"begin":284,"end":287},"obj":"PR_EXT:000027234"},{"id":"T4954","span":{"begin":288,"end":292},"obj":"SO_EXT:sequence_nullness"},{"id":"T4955","span":{"begin":293,"end":297},"obj":"NCBITaxon:10088"}],"text":" mice stained equally well for TRAP and excavated approximately equal numbers of pits of equal size in dentin slices, as visualized by scanning electron microscopy (Figure 6B). These findings suggest that the osteoclasts may not be the primary defect in Sam68−/− mice, as they are in Src null mice [48].\nFigure 6 Ex Vivo Activity of Sam68+/+ and Sam68−/− Osteoblasts and Osteoclasts\nMarrow stromal cells were isolated from the long bones of juvenile mice and maintained under conditions that promote osteoblast differentiation.\n(A) Cultures were fixed in 4% paraformaldehyde after 6 or 18 days and stained in situ for ALP activity and with silver nitrate (von Kossa) to detect mineralized nodules. Sam68−/− cultures stained more intensely for ALP at early and late time points and produced significantly more mineralized nodules after 18 days. Asterisks represent p \u003c 0.01.\n(B) Primary osteoclasts were isolated from the crushed long bones of the same mice and plated on glass coverslips or on dentin slices to quantify numbers and activity, respectively. Osteoclas"}