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When cells were expressed as a function of the bone perimeter (nOB/BP, nOC/BP), there were no statistical differences and the ratio of osteoblasts to osteoclasts was similar in all groups of mice (Table 3).\nTable 3 Histomorphometric Analysis of Long Bones from 4- and 12-Month-Old Mice Circulating levels of serum C-telopeptide (sCTX; Roche Diagnostics, Mannheim, Germany) and ALP showed little difference among the groups, except for a small decrease in 12-month-old Sam68−/− mice and (Dataset S3). Serum estrogen was significantly decreased in all 12-month-old mice regardless of genotype with a reciprocal increase in interleukin-6 levels (Dataset S3). Given the involvement of leptin in regulating body and bone mass, serum leptin was measured in Sam68+/+ and Sam68−/− mice. Twelve-month-old Sam68−/− mice had significantly lower levels than young and old Sam68+/+ mice and young Sam68−/− mice (Dataset S3). Taken together, these observations suggested that preservation of bone ma"}

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{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T4106","span":{"begin":8,"end":13},"obj":"PR_EXT:000009279"},{"id":"T4107","span":{"begin":13,"end":14},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4108","span":{"begin":15,"end":16},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4109","span":{"begin":17,"end":21},"obj":"NCBITaxon:10088"},{"id":"T4110","span":{"begin":75,"end":81},"obj":"UBERON:0002371"},{"id":"T4111","span":{"begin":82,"end":85},"obj":"CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue"},{"id":"T4112","span":{"begin":112,"end":116},"obj":"NCBITaxon:10088"},{"id":"T4113","span":{"begin":127,"end":135},"obj":"SO_EXT:genotype_or_entity_with_genotype"},{"id":"T4114","span":{"begin":152,"end":157},"obj":"CL_GO_EXT:cell"},{"id":"T4115","span":{"begin":194,"end":198},"obj":"UBERON_EXT:bone_element_or_tissue"},{"id":"T4116","span":{"begin":282,"end":293},"obj":"CL:0000062"},{"id":"T4117","span":{"begin":297,"end":308},"obj":"CL:0000092"},{"id":"T4118","span":{"begin":338,"end":342},"obj":"NCBITaxon:10088"},{"id":"T4119","span":{"begin":456,"end":461},"obj":"UBERON:0001977"},{"id":"T4120","span":{"begin":462,"end":463},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T4121","span":{"begin":525,"end":528},"obj":"GO_PR_EXT:alkaline_phosphatase"},{"id":"T4122","span":{"begin":616,"end":621},"obj":"PR_EXT:000009279"},{"id":"T4123","span":{"begin":621,"end":622},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4124","span":{"begin":623,"end":624},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4125","span":{"begin":625,"end":629},"obj":"NCBITaxon:10088"},{"id":"T4126","span":{"begin":648,"end":653},"obj":"UBERON:0001977"},{"id":"T4127","span":{"begin":654,"end":662},"obj":"CHEBI_EXT:50114"},{"id":"T4128","span":{"begin":711,"end":715},"obj":"NCBITaxon:10088"},{"id":"T4129","span":{"begin":730,"end":738},"obj":"SO_EXT:genotype_or_entity_with_genotype"},{"id":"T4130","span":{"begin":769,"end":782},"obj":"PR_EXT:000001393"},{"id":"T4131","span":{"begin":829,"end":835},"obj":"CHEBI_PR_EXT:leptin"},{"id":"T4132","span":{"begin":839,"end":849},"obj":"GO:0065007"},{"id":"T4133","span":{"begin":850,"end":854},"obj":"UBERON_EXT:body"},{"id":"T4134","span":{"begin":859,"end":863},"obj":"UBERON_EXT:bone_element_or_tissue"},{"id":"T4135","span":{"begin":870,"end":875},"obj":"UBERON:0001977"},{"id":"T4136","span":{"begin":876,"end":882},"obj":"CHEBI_PR_EXT:leptin"},{"id":"T4137","span":{"begin":899,"end":904},"obj":"PR_EXT:000009279"},{"id":"T4138","span":{"begin":904,"end":905},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T4139","span":{"begin":906,"end":907},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T4140","span":{"begin":912,"end":917},"obj":"PR_EXT:000009279"},{"id":"T4141","span":{"begin":917,"end":918},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4142","span":{"begin":919,"end":920},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4143","span":{"begin":921,"end":925},"obj":"NCBITaxon:10088"},{"id":"T4144","span":{"begin":944,"end":949},"obj":"PR_EXT:000009279"},{"id":"T4145","span":{"begin":949,"end":950},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4146","span":{"begin":951,"end":952},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4147","span":{"begin":953,"end":957},"obj":"NCBITaxon:10088"},{"id":"T4148","span":{"begin":1008,"end":1013},"obj":"PR_EXT:000009279"},{"id":"T4149","span":{"begin":1013,"end":1014},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T4150","span":{"begin":1015,"end":1016},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T4151","span":{"begin":1017,"end":1021},"obj":"NCBITaxon:10088"},{"id":"T4152","span":{"begin":1032,"end":1037},"obj":"PR_EXT:000009279"},{"id":"T4153","span":{"begin":1037,"end":1038},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4154","span":{"begin":1039,"end":1040},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4155","span":{"begin":1041,"end":1045},"obj":"NCBITaxon:10088"},{"id":"T4156","span":{"begin":1126,"end":1130},"obj":"UBERON_EXT:bone_element_or_tissue"}],"text":"nth-old Sam68−/− mice in which all histomorphometric parameters, including marrow fat, resembled those of young mice of either genotype (Table 3). When cells were expressed as a function of the bone perimeter (nOB/BP, nOC/BP), there were no statistical differences and the ratio of osteoblasts to osteoclasts was similar in all groups of mice (Table 3).\nTable 3 Histomorphometric Analysis of Long Bones from 4- and 12-Month-Old Mice Circulating levels of serum C-telopeptide (sCTX; Roche Diagnostics, Mannheim, Germany) and ALP showed little difference among the groups, except for a small decrease in 12-month-old Sam68−/− mice and (Dataset S3). Serum estrogen was significantly decreased in all 12-month-old mice regardless of genotype with a reciprocal increase in interleukin-6 levels (Dataset S3). Given the involvement of leptin in regulating body and bone mass, serum leptin was measured in Sam68+/+ and Sam68−/− mice. Twelve-month-old Sam68−/− mice had significantly lower levels than young and old Sam68+/+ mice and young Sam68−/− mice (Dataset S3). Taken together, these observations suggested that preservation of bone ma"}