PMC:1315279 / 15169-16373 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1315279","sourcedb":"PMC","sourceid":"1315279","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1315279","text":"Figure 3 Radiologic Assessment of the Femur of Young and Old Sam68+/+ and Sam68−/− Mice\n(A) Mice were given a lethal dose of anesthetic at the indicated times, and contact radiographs of the distal femora were obtained on a Faxitron MX20 equipped with an FPX-2 Imaging system. Representative radiographs of the distal femur of Sam68+/+ (+/+) and Sam68−/− (−/−) mice revealed comparable radiopacity at 4 months (left). At 12 months (right), cortical thinning (arrow) and radiolucency (asterisk) were apparent in the distal femur of +/+ mice but not −/− mice.\n(B) Bones were dissected free of soft tissue and fixed overnight in 4% paraformaldehyde before scanning on a Skyscan 1072 static instrument equipped with 3D Creator analytical software. Representative three-dimensional re-constructions and two-dimensional cross-sectional scans demonstrated similar architecture in the distal femur of Sam68+/+ (+/+) and Sam68−/− (−/−) mice. In keeping with the results from Faxitron x-ray, trabecular bone (asterisk) and cortical thickness (arrow) were reduced in the femur of 12-month-old +/+ mice compared with all other groups. The images are representative of those from five to seven animals in each group.","divisions":[{"label":"label","span":{"begin":0,"end":8}},{"label":"title","span":{"begin":10,"end":88}},{"label":"p","span":{"begin":89,"end":558}}],"tracks":[]}