PMC:1315279 / 12131-12885 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1315279","sourcedb":"PMC","sourceid":"1315279","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1315279","text":"ssette.\n(B) Southern-blot analysis of genomic DNA from wild-type (+/+), heterozygous (+/−), and homozygous (−/−) mice. DNA fragments corresponding to wild-type (4.5 kb) and the targeted (5.5 kb) alleles are illustrated.\n(C) Western blot analysis of Sam68 expression. Protein extracts from wild-type, heterozygous, and homozygous cells subjected to immunoblot analyses using normal rabbit serum, anti-Sam68 AD1 antibody, the peptide antibody AD1 preabsorbed with the immunogenic peptide corresponding to amino acids 330–348 of mouse Sam68, anti-Sam68 Sc333 antibody that recognizes the C-terminal 20 amino acids of Sam68, and anti-actin antibodies as loading control. The migration of the molecular mass markers is known on the left in kDa.\nTable 1 Proge","divisions":[{"label":"p","span":{"begin":8,"end":219}},{"label":"p","span":{"begin":220,"end":739}},{"label":"label","span":{"begin":740,"end":747}}],"tracks":[]}