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PMC:1310901 / 8718-10201 JSONTXT

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Id Subject Object Predicate Lexical cue
T4922 722-727 Protein denotes IRF-4

sentences

Id Subject Object Predicate Lexical cue
T4350 0-42 Sentence denotes Methylation-specific restriction-PCR-assay
T4351 43-107 Sentence denotes DNA was extracted with a commercial kit (Qiagen) as recommended.
T4352 108-351 Sentence denotes Since the restriction ability of several endonucleases is inhibited by methylation of their target sequence, we used methylation-sensitive enzymes HpaII and HaeII-isochizomer Bsp143II and Bsh1236I (MBI Fermentas, St Leon-Rot, Germany) (20,24).
T4353 352-481 Sentence denotes As control the methylation-resistant enzyme MspI and an enzyme with no recognition site in the target promoter, EcoRI, were used.
T4354 482-605 Sentence denotes DNA (0.8 µg) was digested by 40 U the respective enzyme for 6 h and, to ensure complete cleavage, additional 20 U for 16 h.
T4355 606-774 Sentence denotes Thereafter 100 ng of digested DNA was used to a PCR amplification of two fragments (F1 and F2) spanning part of the IRF-4 promoter (30) (GenBank U52683; see Figure 3A).
T4356 775-820 Sentence denotes The sequences of the primers were F1-forward:
T4357 821-913 Sentence denotes 5′-TTGAGATGGAGTCTTGCTCTGT-3′, F1-reverse: ATCACTTCCAGACTTCAGTTCACCT-3′ (341 bp); F2-forward:
T4358 914-958 Sentence denotes 5′-AAGGTGAACTGAAGTCTGGAAGTGA-3′, F2-reverse:
T4359 959-998 Sentence denotes 5′-CCAGGACCTCAGGAGGCCAGTCA-3′ (474 bp).
T4360 999-1047 Sentence denotes The PCR conditions were described elsewhere (3).
T4361 1048-1118 Sentence denotes PCR was performed with an annealing temperature of 62°C and 35 cycles.
T4362 1119-1319 Sentence denotes When DNA was methylated at specific sites, the sensitive enzymes were not able to digest the DNA and amplification took place; in case of no methylation, DNA was digested and no product was generated.
T4363 1320-1431 Sentence denotes The PCR products were electrophoresed on a 3% agarose gel, were stained with ethidium bromide and photographed.
T4364 1432-1483 Sentence denotes PCR products were verified by automated sequencing.
T64 0-42 Sentence denotes Methylation-specific restriction-PCR-assay
T65 43-107 Sentence denotes DNA was extracted with a commercial kit (Qiagen) as recommended.
T66 108-351 Sentence denotes Since the restriction ability of several endonucleases is inhibited by methylation of their target sequence, we used methylation-sensitive enzymes HpaII and HaeII-isochizomer Bsp143II and Bsh1236I (MBI Fermentas, St Leon-Rot, Germany) (20,24).
T67 352-481 Sentence denotes As control the methylation-resistant enzyme MspI and an enzyme with no recognition site in the target promoter, EcoRI, were used.
T68 482-605 Sentence denotes DNA (0.8 µg) was digested by 40 U the respective enzyme for 6 h and, to ensure complete cleavage, additional 20 U for 16 h.
T69 606-774 Sentence denotes Thereafter 100 ng of digested DNA was used to a PCR amplification of two fragments (F1 and F2) spanning part of the IRF-4 promoter (30) (GenBank U52683; see Figure 3A).
T70 775-820 Sentence denotes The sequences of the primers were F1-forward:
T71 821-913 Sentence denotes 5′-TTGAGATGGAGTCTTGCTCTGT-3′, F1-reverse: ATCACTTCCAGACTTCAGTTCACCT-3′ (341 bp); F2-forward:
T72 914-958 Sentence denotes 5′-AAGGTGAACTGAAGTCTGGAAGTGA-3′, F2-reverse:
T73 959-998 Sentence denotes 5′-CCAGGACCTCAGGAGGCCAGTCA-3′ (474 bp).
T74 999-1047 Sentence denotes The PCR conditions were described elsewhere (3).
T75 1048-1118 Sentence denotes PCR was performed with an annealing temperature of 62°C and 35 cycles.
T76 1119-1319 Sentence denotes When DNA was methylated at specific sites, the sensitive enzymes were not able to digest the DNA and amplification took place; in case of no methylation, DNA was digested and no product was generated.
T77 1320-1431 Sentence denotes The PCR products were electrophoresed on a 3% agarose gel, were stained with ethidium bromide and photographed.
T78 1432-1483 Sentence denotes PCR products were verified by automated sequencing.

2_test

Id Subject Object Predicate Lexical cue
16396836-7938018-76538186 344-346 7938018 denotes 20
16396836-9389709-76538187 347-349 9389709 denotes 24
16396836-8921401-76538188 738-740 8921401 denotes 30
16396836-11013272-76538189 1044-1045 11013272 denotes 3

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4371 0-20 JJ denotes Methylation-specific
T4372 21-42 NN denotes restriction-PCR-assay
T4373 43-46 NN denotes DNA
T4374 47-50 VB denotes was
T4375 51-60 VB denotes extracted
T4376 61-65 IN denotes with
T4377 66-67 DT denotes a
T4378 68-78 JJ denotes commercial
T4379 79-82 NN denotes kit
T4380 83-84 -LRB- denotes (
T4381 84-90 NN denotes Qiagen
T4382 90-91 -RRB- denotes )
T4383 92-94 IN denotes as
T4384 95-106 VB denotes recommended
T4385 108-113 IN denotes Since
T4386 114-117 DT denotes the
T4387 118-129 NN denotes restriction
T4388 130-137 NN denotes ability
T4389 138-140 IN denotes of
T4390 141-148 JJ denotes several
T4391 149-162 NN denotes endonucleases
T4392 163-165 VB denotes is
T4393 166-175 VB denotes inhibited
T4394 176-178 IN denotes by
T4395 179-190 NN denotes methylation
T4396 191-193 IN denotes of
T4397 194-199 PRP-DOLLAR- denotes their
T4398 200-206 NN denotes target
T4399 207-215 NN denotes sequence
T4400 215-216 -COMMA- denotes ,
T4401 217-219 PRP denotes we
T4402 220-224 VB denotes used
T4403 225-246 JJ denotes methylation-sensitive
T4404 247-254 NN denotes enzymes
T4405 255-260 NN denotes HpaII
T4406 261-264 CC denotes and
T4407 265-282 NN denotes HaeII-isochizomer
T4408 283-291 NN denotes Bsp143II
T4409 292-295 CC denotes and
T4410 296-304 NN denotes Bsh1236I
T4411 305-306 -LRB- denotes (
T4412 306-309 NNP denotes MBI
T4413 310-319 NNP denotes Fermentas
T4414 319-320 -COMMA- denotes ,
T4415 321-323 NNP denotes St
T4416 324-332 NNP denotes Leon-Rot
T4417 332-333 -COMMA- denotes ,
T4418 334-341 NNP denotes Germany
T4419 341-342 -RRB- denotes )
T4420 343-344 -LRB- denotes (
T4421 344-349 CD denotes 20,24
T4422 349-350 -RRB- denotes )
T4423 352-354 IN denotes As
T4424 355-362 VB denotes control
T4425 363-366 DT denotes the
T4426 367-388 JJ denotes methylation-resistant
T4427 389-395 NN denotes enzyme
T4428 396-400 NN denotes MspI
T4429 401-404 CC denotes and
T4430 405-407 DT denotes an
T4431 408-414 NN denotes enzyme
T4432 415-419 IN denotes with
T4433 420-422 DT denotes no
T4434 423-434 NN denotes recognition
T4435 435-439 NN denotes site
T4436 440-442 IN denotes in
T4437 443-446 DT denotes the
T4438 447-453 NN denotes target
T4439 454-462 NN denotes promoter
T4440 462-463 -COMMA- denotes ,
T4441 464-469 NN denotes EcoRI
T4442 469-470 -COMMA- denotes ,
T4443 471-475 VB denotes were
T4444 476-480 VB denotes used
T4445 482-485 NN denotes DNA
T4446 486-487 -LRB- denotes (
T4447 487-490 CD denotes 0.8
T4448 491-493 NN denotes µg
T4449 493-494 -RRB- denotes )
T4450 495-498 VB denotes was
T4451 499-507 VB denotes digested
T4452 508-510 IN denotes by
T4453 511-513 CD denotes 40
T4454 514-515 NN denotes U
T4455 516-519 DT denotes the
T4456 520-530 JJ denotes respective
T4457 531-537 NN denotes enzyme
T4458 538-541 IN denotes for
T4459 542-543 CD denotes 6
T4460 544-545 NN denotes h
T4461 546-549 CC denotes and
T4462 549-550 -COMMA- denotes ,
T4463 551-553 TO denotes to
T4464 554-560 VB denotes ensure
T4465 561-569 JJ denotes complete
T4466 570-578 NN denotes cleavage
T4467 578-579 -COMMA- denotes ,
T4468 580-590 JJ denotes additional
T4469 591-593 CD denotes 20
T4470 594-595 NN denotes U
T4471 596-599 IN denotes for
T4472 600-602 CD denotes 16
T4473 603-604 NN denotes h
T4474 606-616 RB denotes Thereafter
T4475 617-620 CD denotes 100
T4476 621-623 NN denotes ng
T4477 624-626 IN denotes of
T4478 627-635 JJ denotes digested
T4479 636-639 NN denotes DNA
T4480 640-643 VB denotes was
T4481 644-648 VB denotes used
T4482 649-651 TO denotes to
T4483 652-653 DT denotes a
T4484 654-657 NN denotes PCR
T4485 658-671 NN denotes amplification
T4486 672-674 IN denotes of
T4487 675-678 CD denotes two
T4488 679-688 NN denotes fragments
T4489 689-690 -LRB- denotes (
T4490 690-692 NN denotes F1
T4491 693-696 CC denotes and
T4492 697-699 NN denotes F2
T4493 699-700 -RRB- denotes )
T4494 701-709 VB denotes spanning
T4495 710-714 NN denotes part
T4496 715-717 IN denotes of
T4497 718-721 DT denotes the
T4498 722-727 NN denotes IRF-4
T4499 728-736 NN denotes promoter
T4500 737-738 -LRB- denotes (
T4501 738-740 CD denotes 30
T4502 740-741 -RRB- denotes )
T4503 742-743 -LRB- denotes (
T4504 743-750 NN denotes GenBank
T4505 751-757 NN denotes U52683
T4506 757-758 -COLON- denotes ;
T4507 759-762 VB denotes see
T4508 763-769 NN denotes Figure
T4509 770-772 NN denotes 3A
T4510 772-773 -RRB- denotes )
T4511 775-778 DT denotes The
T4512 779-788 NN denotes sequences
T4513 789-791 IN denotes of
T4514 792-795 DT denotes the
T4515 796-803 NN denotes primers
T4516 804-808 VB denotes were
T4517 809-819 JJ denotes F1-forward
T4518 819-820 -COLON- denotes :
T4519 821-849 JJ denotes 5′-TTGAGATGGAGTCTTGCTCTGT-3′
T4520 849-850 -COMMA- denotes ,
T4521 851-861 JJ denotes F1-reverse
T4522 861-862 -COLON- denotes :
T4523 863-891 NN denotes ATCACTTCCAGACTTCAGTTCACCT-3′
T4524 892-893 -LRB- denotes (
T4525 893-896 CD denotes 341
T4526 897-899 NN denotes bp
T4527 899-900 -RRB- denotes )
T4528 900-901 -COLON- denotes ;
T4529 902-912 JJ denotes F2-forward
T4530 912-913 -COLON- denotes :
T4531 914-945 JJ denotes 5′-AAGGTGAACTGAAGTCTGGAAGTGA-3′
T4532 945-946 -COMMA- denotes ,
T4533 947-957 JJ denotes F2-reverse
T4534 957-958 -COLON- denotes :
T4535 959-988 NN denotes 5′-CCAGGACCTCAGGAGGCCAGTCA-3′
T4536 989-990 -LRB- denotes (
T4537 990-993 CD denotes 474
T4538 994-996 NN denotes bp
T4539 996-997 -RRB- denotes )
T4540 999-1002 DT denotes The
T4541 1003-1006 NN denotes PCR
T4542 1007-1017 NN denotes conditions
T4543 1018-1022 VB denotes were
T4544 1023-1032 VB denotes described
T4545 1033-1042 RB denotes elsewhere
T4546 1043-1044 -LRB- denotes (
T4547 1044-1045 CD denotes 3
T4548 1045-1046 -RRB- denotes )
T4549 1048-1051 NN denotes PCR
T4550 1052-1055 VB denotes was
T4551 1056-1065 VB denotes performed
T4552 1066-1070 IN denotes with
T4553 1071-1073 DT denotes an
T4554 1074-1083 JJ denotes annealing
T4555 1084-1095 NN denotes temperature
T4556 1096-1098 IN denotes of
T4557 1099-1103 NN denotes 62°C
T4558 1104-1107 CC denotes and
T4559 1108-1110 CD denotes 35
T4560 1111-1117 NN denotes cycles
T4561 1119-1123 WRB denotes When
T4562 1124-1127 NN denotes DNA
T4563 1128-1131 VB denotes was
T4564 1132-1142 VB denotes methylated
T4565 1143-1145 IN denotes at
T4566 1146-1154 JJ denotes specific
T4567 1155-1160 NN denotes sites
T4568 1160-1161 -COMMA- denotes ,
T4569 1162-1165 DT denotes the
T4570 1166-1175 JJ denotes sensitive
T4571 1176-1183 NN denotes enzymes
T4572 1184-1188 VB denotes were
T4573 1189-1192 RB denotes not
T4574 1193-1197 JJ denotes able
T4575 1198-1200 TO denotes to
T4576 1201-1207 VB denotes digest
T4577 1208-1211 DT denotes the
T4578 1212-1215 NN denotes DNA
T4579 1216-1219 CC denotes and
T4580 1220-1233 NN denotes amplification
T4581 1234-1238 VB denotes took
T4582 1239-1244 NN denotes place
T4583 1244-1245 -COLON- denotes ;
T4584 1246-1248 IN denotes in
T4585 1249-1253 NN denotes case
T4586 1254-1256 IN denotes of
T4587 1257-1259 DT denotes no
T4588 1260-1271 NN denotes methylation
T4589 1271-1272 -COMMA- denotes ,
T4590 1273-1276 NN denotes DNA
T4591 1277-1280 VB denotes was
T4592 1281-1289 VB denotes digested
T4593 1290-1293 CC denotes and
T4594 1294-1296 DT denotes no
T4595 1297-1304 NN denotes product
T4596 1305-1308 VB denotes was
T4597 1309-1318 VB denotes generated
T4598 1320-1323 DT denotes The
T4599 1324-1327 NN denotes PCR
T4600 1328-1336 NN denotes products
T4601 1337-1341 VB denotes were
T4602 1342-1357 VB denotes electrophoresed
T4603 1358-1360 IN denotes on
T4604 1361-1362 DT denotes a
T4605 1363-1364 CD denotes 3
T4606 1364-1365 NN denotes %
T4607 1366-1373 NN denotes agarose
T4608 1374-1377 NN denotes gel
T4609 1377-1378 -COMMA- denotes ,
T4610 1379-1383 VB denotes were
T4611 1384-1391 VB denotes stained
T4612 1392-1396 IN denotes with
T4613 1397-1405 NN denotes ethidium
T4614 1406-1413 NN denotes bromide
T4615 1414-1417 CC denotes and
T4616 1418-1430 VB denotes photographed
T4617 1432-1435 NN denotes PCR
T4618 1436-1444 NN denotes products
T4619 1445-1449 VB denotes were
T4620 1450-1458 VB denotes verified
T4621 1459-1461 IN denotes by
T4622 1462-1471 VB denotes automated
T4623 1472-1482 NN denotes sequencing
R3523 T4372 T4371 arg1Of restriction-PCR-assay,Methylation-specific
R3524 T4373 T4374 arg1Of DNA,was
R3525 T4373 T4375 arg2Of DNA,extracted
R3526 T4375 T4374 arg2Of extracted,was
R3527 T4375 T4376 arg1Of extracted,with
R3528 T4375 T4383 arg1Of extracted,as
R3529 T4379 T4376 arg2Of kit,with
R3530 T4379 T4377 arg1Of kit,a
R3531 T4379 T4378 arg1Of kit,commercial
R3532 T4379 T4380 arg1Of kit,(
R3533 T4381 T4380 arg2Of Qiagen,(
R3534 T4382 T4380 arg3Of ),(
R3535 T4384 T4383 arg2Of recommended,as
R3536 T4388 T4386 arg1Of ability,the
R3537 T4388 T4387 arg1Of ability,restriction
R3538 T4388 T4389 arg1Of ability,of
R3539 T4388 T4392 arg1Of ability,is
R3540 T4388 T4393 arg2Of ability,inhibited
R3541 T4391 T4389 arg2Of endonucleases,of
R3542 T4391 T4390 arg1Of endonucleases,several
R3543 T4393 T4385 arg2Of inhibited,Since
R3544 T4393 T4392 arg2Of inhibited,is
R3545 T4395 T4393 arg1Of methylation,inhibited
R3546 T4395 T4394 arg2Of methylation,by
R3547 T4395 T4396 arg1Of methylation,of
R3548 T4399 T4396 arg2Of sequence,of
R3549 T4399 T4397 arg1Of sequence,their
R3550 T4399 T4398 arg1Of sequence,target
R3551 T4401 T4402 arg1Of we,used
R3552 T4402 T4385 arg1Of used,Since
R3553 T4402 T4400 arg1Of used,","
R3554 T4405 T4403 arg1Of HpaII,methylation-sensitive
R3555 T4405 T4404 arg1Of HpaII,enzymes
R3556 T4405 T4406 arg1Of HpaII,and
R3557 T4406 T4409 arg1Of and,and
R3558 T4408 T4406 arg2Of Bsp143II,and
R3559 T4408 T4407 arg1Of Bsp143II,HaeII-isochizomer
R3560 T4409 T4402 arg2Of and,used
R3561 T4409 T4420 arg1Of and,(
R3562 T4410 T4409 arg2Of Bsh1236I,and
R3563 T4410 T4411 arg1Of Bsh1236I,(
R3564 T4413 T4411 arg2Of Fermentas,(
R3565 T4413 T4412 arg1Of Fermentas,MBI
R3566 T4413 T4414 arg1Of Fermentas,","
R3567 T4416 T4414 arg2Of Leon-Rot,","
R3568 T4416 T4415 arg1Of Leon-Rot,St
R3569 T4416 T4417 arg1Of Leon-Rot,","
R3570 T4418 T4417 arg2Of Germany,","
R3571 T4419 T4411 arg3Of ),(
R3572 T4421 T4420 arg2Of "20,24",(
R3573 T4422 T4420 arg3Of ),(
R3574 T4424 T4423 arg2Of control,As
R3575 T4424 T4432 arg1Of control,with
R3576 T4428 T4425 arg1Of MspI,the
R3577 T4428 T4426 arg1Of MspI,methylation-resistant
R3578 T4428 T4427 arg1Of MspI,enzyme
R3579 T4428 T4429 arg1Of MspI,and
R3580 T4429 T4424 arg2Of and,control
R3581 T4431 T4429 arg2Of enzyme,and
R3582 T4431 T4430 arg1Of enzyme,an
R3583 T4435 T4432 arg2Of site,with
R3584 T4435 T4433 arg1Of site,no
R3585 T4435 T4434 arg1Of site,recognition
R3586 T4435 T4436 arg1Of site,in
R3587 T4439 T4436 arg2Of promoter,in
R3588 T4439 T4437 arg1Of promoter,the
R3589 T4439 T4438 arg1Of promoter,target
R3590 T4441 T4443 arg1Of EcoRI,were
R3591 T4441 T4444 arg2Of EcoRI,used
R3592 T4444 T4423 arg1Of used,As
R3593 T4444 T4440 arg1Of used,","
R3594 T4444 T4442 arg1Of used,","
R3595 T4444 T4443 arg2Of used,were
R3596 T4445 T4446 arg1Of DNA,(
R3597 T4445 T4450 arg1Of DNA,was
R3598 T4445 T4451 arg2Of DNA,digested
R3599 T4448 T4446 arg2Of µg,(
R3600 T4448 T4447 arg1Of µg,0.8
R3601 T4449 T4446 arg3Of ),(
R3602 T4451 T4450 arg2Of digested,was
R3603 T4451 T4462 arg1Of digested,","
R3604 T4451 T4463 modOf digested,to
R3605 T4451 T4467 arg1Of digested,","
R3606 T4451 T4471 arg1Of digested,for
R3607 T4454 T4453 arg1Of U,40
R3608 T4457 T4451 arg1Of enzyme,digested
R3609 T4457 T4452 arg2Of enzyme,by
R3610 T4457 T4454 arg1Of enzyme,U
R3611 T4457 T4455 arg1Of enzyme,the
R3612 T4457 T4456 arg1Of enzyme,respective
R3613 T4457 T4458 arg1Of enzyme,for
R3614 T4457 T4461 arg1Of enzyme,and
R3615 T4460 T4458 arg2Of h,for
R3616 T4460 T4459 arg1Of h,6
R3617 T4464 T4463 arg1Of ensure,to
R3618 T4466 T4464 arg2Of cleavage,ensure
R3619 T4466 T4465 arg1Of cleavage,complete
R3620 T4470 T4468 arg1Of U,additional
R3621 T4470 T4469 arg1Of U,20
R3622 T4471 T4470 arg1Of for,U
R3623 T4473 T4471 arg2Of h,for
R3624 T4473 T4472 arg1Of h,16
R3625 T4475 T4474 arg1Of 100,Thereafter
R3626 T4476 T4475 arg1Of ng,100
R3627 T4476 T4477 arg1Of ng,of
R3628 T4476 T4480 arg1Of ng,was
R3629 T4476 T4481 arg2Of ng,used
R3630 T4479 T4477 arg2Of DNA,of
R3631 T4479 T4478 arg1Of DNA,digested
R3632 T4481 T4480 arg2Of used,was
R3633 T4481 T4482 arg1Of used,to
R3634 T4481 T4510 arg1Of used,)
R3635 T4485 T4482 arg2Of amplification,to
R3636 T4485 T4483 arg1Of amplification,a
R3637 T4485 T4484 arg1Of amplification,PCR
R3638 T4485 T4486 arg1Of amplification,of
R3639 T4485 T4489 arg1Of amplification,(
R3640 T4485 T4494 arg1Of amplification,spanning
R3641 T4485 T4507 modOf amplification,see
R3642 T4488 T4486 arg2Of fragments,of
R3643 T4488 T4487 arg1Of fragments,two
R3644 T4490 T4491 arg1Of F1,and
R3645 T4491 T4489 arg2Of and,(
R3646 T4492 T4491 arg2Of F2,and
R3647 T4493 T4489 arg3Of ),(
R3648 T4494 T4506 arg1Of spanning,;
R3649 T4495 T4494 arg2Of part,spanning
R3650 T4495 T4496 arg1Of part,of
R3651 T4501 T4500 arg2Of 30,(
R3652 T4502 T4500 arg3Of ),(
R3653 T4505 T4496 arg2Of U52683,of
R3654 T4505 T4497 arg1Of U52683,the
R3655 T4505 T4498 arg1Of U52683,IRF-4
R3656 T4505 T4499 arg1Of U52683,promoter
R3657 T4505 T4500 arg1Of U52683,(
R3658 T4505 T4503 arg1Of U52683,(
R3659 T4505 T4504 arg1Of U52683,GenBank
R3660 T4509 T4507 arg2Of 3A,see
R3661 T4509 T4508 arg1Of 3A,Figure
R3662 T4512 T4511 arg1Of sequences,The
R3663 T4512 T4513 arg1Of sequences,of
R3664 T4512 T4516 arg1Of sequences,were
R3665 T4512 T4517 arg1Of sequences,F1-forward
R3666 T4515 T4513 arg2Of primers,of
R3667 T4515 T4514 arg1Of primers,the
R3668 T4516 T4518 arg1Of were,:
R3669 T4517 T4516 arg2Of F1-forward,were
R3670 T4519 T4520 arg1Of 5′-TTGAGATGGAGTCTTGCTCTGT-3′,","
R3671 T4520 T4522 arg1Of ",",:
R3672 T4521 T4520 arg2Of F1-reverse,","
R3673 T4523 T4519 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,5′-TTGAGATGGAGTCTTGCTCTGT-3′
R3674 T4523 T4521 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,F1-reverse
R3675 T4523 T4524 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,(
R3676 T4523 T4528 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,;
R3677 T4526 T4524 arg2Of bp,(
R3678 T4526 T4525 arg1Of bp,341
R3679 T4527 T4524 arg3Of ),(
R3680 T4529 T4528 arg2Of F2-forward,;
R3681 T4529 T4530 arg1Of F2-forward,:
R3682 T4531 T4532 arg1Of 5′-AAGGTGAACTGAAGTCTGGAAGTGA-3′,","
R3683 T4532 T4534 arg1Of ",",:
R3684 T4533 T4532 arg2Of F2-reverse,","
R3685 T4535 T4536 arg1Of 5′-CCAGGACCTCAGGAGGCCAGTCA-3′,(
R3686 T4538 T4536 arg2Of bp,(
R3687 T4538 T4537 arg1Of bp,474
R3688 T4539 T4536 arg3Of ),(
R3689 T4542 T4540 arg1Of conditions,The
R3690 T4542 T4541 arg1Of conditions,PCR
R3691 T4542 T4543 arg1Of conditions,were
R3692 T4542 T4544 arg2Of conditions,described
R3693 T4544 T4543 arg2Of described,were
R3694 T4544 T4545 arg1Of described,elsewhere
R3695 T4545 T4546 arg1Of elsewhere,(
R3696 T4547 T4546 arg2Of 3,(
R3697 T4548 T4546 arg3Of ),(
R3698 T4549 T4550 arg1Of PCR,was
R3699 T4549 T4551 arg2Of PCR,performed
R3700 T4551 T4550 arg2Of performed,was
R3701 T4551 T4552 arg1Of performed,with
R3702 T4555 T4552 arg2Of temperature,with
R3703 T4555 T4553 arg1Of temperature,an
R3704 T4555 T4554 arg1Of temperature,annealing
R3705 T4555 T4556 arg1Of temperature,of
R3706 T4557 T4558 arg1Of 62°C,and
R3707 T4558 T4556 arg2Of and,of
R3708 T4560 T4558 arg2Of cycles,and
R3709 T4560 T4559 arg1Of cycles,35
R3710 T4562 T4563 arg1Of DNA,was
R3711 T4562 T4564 arg2Of DNA,methylated
R3712 T4564 T4561 arg2Of methylated,When
R3713 T4564 T4563 arg2Of methylated,was
R3714 T4564 T4565 arg1Of methylated,at
R3715 T4567 T4565 arg2Of sites,at
R3716 T4567 T4566 arg1Of sites,specific
R3717 T4571 T4569 arg1Of enzymes,the
R3718 T4571 T4570 arg1Of enzymes,sensitive
R3719 T4571 T4572 arg1Of enzymes,were
R3720 T4571 T4574 arg1Of enzymes,able
R3721 T4571 T4576 arg1Of enzymes,digest
R3722 T4572 T4573 arg1Of were,not
R3723 T4572 T4579 arg1Of were,and
R3724 T4574 T4572 arg2Of able,were
R3725 T4576 T4574 arg2Of digest,able
R3726 T4576 T4575 arg1Of digest,to
R3727 T4578 T4576 arg2Of DNA,digest
R3728 T4578 T4577 arg1Of DNA,the
R3729 T4579 T4561 arg1Of and,When
R3730 T4579 T4568 arg1Of and,","
R3731 T4579 T4583 arg1Of and,;
R3732 T4580 T4581 arg1Of amplification,took
R3733 T4581 T4579 arg2Of took,and
R3734 T4582 T4581 arg2Of place,took
R3735 T4585 T4584 arg2Of case,in
R3736 T4585 T4586 arg1Of case,of
R3737 T4588 T4586 arg2Of methylation,of
R3738 T4588 T4587 arg1Of methylation,no
R3739 T4590 T4591 arg1Of DNA,was
R3740 T4590 T4592 arg2Of DNA,digested
R3741 T4592 T4591 arg2Of digested,was
R3742 T4592 T4593 arg1Of digested,and
R3743 T4593 T4583 arg2Of and,;
R3744 T4593 T4584 arg1Of and,in
R3745 T4593 T4589 arg1Of and,","
R3746 T4595 T4594 arg1Of product,no
R3747 T4595 T4596 arg1Of product,was
R3748 T4595 T4597 arg2Of product,generated
R3749 T4597 T4593 arg2Of generated,and
R3750 T4597 T4596 arg2Of generated,was
R3751 T4600 T4598 arg1Of products,The
R3752 T4600 T4599 arg1Of products,PCR
R3753 T4600 T4601 arg1Of products,were
R3754 T4600 T4602 arg2Of products,electrophoresed
R3755 T4600 T4610 arg1Of products,were
R3756 T4600 T4611 arg2Of products,stained
R3757 T4600 T4616 arg2Of products,photographed
R3758 T4602 T4601 arg2Of electrophoresed,were
R3759 T4602 T4603 arg1Of electrophoresed,on
R3760 T4602 T4609 arg1Of electrophoresed,","
R3761 T4605 T4606 arg1Of 3,%
R3762 T4608 T4603 arg2Of gel,on
R3763 T4608 T4604 arg1Of gel,a
R3764 T4608 T4605 arg1Of gel,3
R3765 T4608 T4607 arg1Of gel,agarose
R3766 T4611 T4612 arg1Of stained,with
R3767 T4611 T4615 arg1Of stained,and
R3768 T4614 T4612 arg2Of bromide,with
R3769 T4614 T4613 arg1Of bromide,ethidium
R3770 T4615 T4609 arg2Of and,","
R3771 T4615 T4610 arg2Of and,were
R3772 T4616 T4615 arg2Of photographed,and
R3773 T4618 T4617 arg1Of products,PCR
R3774 T4618 T4619 arg1Of products,were
R3775 T4618 T4620 arg2Of products,verified
R3776 T4620 T4619 arg2Of verified,were
R3777 T4623 T4620 arg1Of sequencing,verified
R3778 T4623 T4621 arg2Of sequencing,by
R3779 T4623 T4622 arg2Of sequencing,automated

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4626 0-20 JJ denotes Methylation-specific
T4627 21-42 JJ denotes restriction-PCR-assay
T4628 43-46 NN denotes DNA
T4629 47-50 VBD denotes was
T4630 51-60 VBN denotes extracted
T4631 61-65 IN denotes with
T4632 66-67 DT denotes a
T4633 68-78 JJ denotes commercial
T4634 79-82 NN denotes kit
T4635 83-84 -LRB- denotes (
T4636 84-90 NNP denotes Qiagen
T4637 90-91 -RRB- denotes )
T4638 92-94 IN denotes as
T4639 95-106 VBN denotes recommended
T4640 106-107 . denotes .
T4641 108-113 IN denotes Since
T4642 114-117 DT denotes the
T4643 118-129 NN denotes restriction
T4644 130-137 NN denotes ability
T4645 138-140 IN denotes of
T4646 141-148 JJ denotes several
T4647 149-162 NNS denotes endonucleases
T4648 163-165 VBZ denotes is
T4649 166-175 VBN denotes inhibited
T4650 176-178 IN denotes by
T4651 179-190 NN denotes methylation
T4652 191-193 IN denotes of
T4653 194-199 PRP$ denotes their
T4654 200-206 NN denotes target
T4655 207-215 NN denotes sequence
T4656 215-216 , denotes ,
T4657 217-219 PRP denotes we
T4658 220-224 VBD denotes used
T4659 225-246 JJ denotes methylation-sensitive
T4660 247-254 NNS denotes enzymes
T4661 255-260 NNP denotes HpaII
T4662 261-264 CC denotes and
T4663 265-282 NNP denotes HaeII-isochizomer
T4664 283-291 NNP denotes Bsp143II
T4665 292-295 CC denotes and
T4666 296-304 NNP denotes Bsh1236I
T4667 305-306 -LRB- denotes (
T4668 306-309 NNP denotes MBI
T4669 310-319 NNP denotes Fermentas
T4670 319-320 , denotes ,
T4671 321-323 NNP denotes St
T4672 324-332 NNP denotes Leon-Rot
T4673 332-333 , denotes ,
T4674 334-341 NNP denotes Germany
T4675 341-342 -RRB- denotes )
T4676 343-344 -LRB- denotes (
T4677 344-349 CD denotes 20,24
T4678 349-350 -RRB- denotes )
T4679 350-351 . denotes .
T4680 352-354 IN denotes As
T4681 355-362 NN denotes control
T4682 363-366 DT denotes the
T4683 367-388 JJ denotes methylation-resistant
T4684 389-395 NN denotes enzyme
T4685 396-400 NNP denotes MspI
T4686 401-404 CC denotes and
T4687 405-407 DT denotes an
T4688 408-414 NN denotes enzyme
T4689 415-419 IN denotes with
T4690 420-422 DT denotes no
T4691 423-434 NN denotes recognition
T4692 435-439 NN denotes site
T4693 440-442 IN denotes in
T4694 443-446 DT denotes the
T4695 447-453 NN denotes target
T4696 454-462 NN denotes promoter
T4697 462-463 , denotes ,
T4698 464-469 NNP denotes EcoRI
T4699 469-470 , denotes ,
T4700 471-475 VBD denotes were
T4701 476-480 VBN denotes used
T4702 480-481 . denotes .
T4703 482-485 NNP denotes DNA
T4704 486-487 -LRB- denotes (
T4705 487-490 CD denotes 0.8
T4706 491-493 NN denotes µg
T4707 493-494 -RRB- denotes )
T4708 495-498 VBD denotes was
T4709 499-507 VBN denotes digested
T4710 508-510 IN denotes by
T4711 511-513 CD denotes 40
T4712 514-515 NNP denotes U
T4713 516-519 DT denotes the
T4714 520-530 JJ denotes respective
T4715 531-537 NN denotes enzyme
T4716 538-541 IN denotes for
T4717 542-543 CD denotes 6
T4718 544-545 NN denotes h
T4719 546-549 CC denotes and
T4720 549-550 , denotes ,
T4721 551-553 TO denotes to
T4722 554-560 VB denotes ensure
T4723 561-569 JJ denotes complete
T4724 570-578 NN denotes cleavage
T4725 578-579 , denotes ,
T4726 580-590 JJ denotes additional
T4727 591-593 CD denotes 20
T4728 594-595 NNP denotes U
T4729 596-599 IN denotes for
T4730 600-602 CD denotes 16
T4731 603-605 NN denotes h.
T4732 606-616 RB denotes Thereafter
T4733 617-620 CD denotes 100
T4734 621-623 NN denotes ng
T4735 624-626 IN denotes of
T4736 627-635 VBN denotes digested
T4737 636-639 NN denotes DNA
T4738 640-643 VBD denotes was
T4739 644-648 VBN denotes used
T4740 649-651 TO denotes to
T4741 652-653 DT denotes a
T4742 654-657 NNP denotes PCR
T4743 658-671 NN denotes amplification
T4744 672-674 IN denotes of
T4745 675-678 CD denotes two
T4746 679-688 NNS denotes fragments
T4747 689-690 -LRB- denotes (
T4748 690-692 CD denotes F1
T4749 693-696 CC denotes and
T4750 697-699 CD denotes F2
T4751 699-700 -RRB- denotes )
T4752 701-709 VBG denotes spanning
T4753 710-714 NN denotes part
T4754 715-717 IN denotes of
T4755 718-721 DT denotes the
T4756 722-727 NN denotes IRF-4
T4757 728-736 NN denotes promoter
T4758 737-738 -LRB- denotes (
T4759 738-740 CD denotes 30
T4760 740-741 -RRB- denotes )
T4761 742-743 -LRB- denotes (
T4762 743-750 NNP denotes GenBank
T4763 751-757 NNP denotes U52683
T4764 757-758 : denotes ;
T4765 759-762 VB denotes see
T4766 763-769 NN denotes Figure
T4767 770-772 NN denotes 3A
T4768 772-773 -RRB- denotes )
T4769 773-774 . denotes .
T4770 775-778 DT denotes The
T4771 779-788 NNS denotes sequences
T4772 789-791 IN denotes of
T4773 792-795 DT denotes the
T4774 796-803 NNS denotes primers
T4775 804-808 VBD denotes were
T4776 809-819 JJ denotes F1-forward
T4777 819-820 : denotes :
T4778 821-822 CD denotes 5
T4779 822-823 SYM denotes ′
T4780 823-824 : denotes -
T4781 824-848 NN denotes TTGAGATGGAGTCTTGCTCTGT-3
T4782 848-849 NN denotes ′
T4783 849-850 , denotes ,
T4784 851-861 NN denotes F1-reverse
T4785 861-862 : denotes :
T4786 863-890 JJ denotes ATCACTTCCAGACTTCAGTTCACCT-3
T4787 890-891 NN denotes ′
T4788 892-893 -LRB- denotes (
T4789 893-896 CD denotes 341
T4790 897-899 NN denotes bp
T4791 899-900 -RRB- denotes )
T4792 900-901 : denotes ;
T4793 902-912 NNP denotes F2-forward
T4794 912-913 : denotes :
T4795 914-915 CD denotes 5
T4796 915-916 SYM denotes ′
T4797 916-917 : denotes -
T4798 917-944 NN denotes AAGGTGAACTGAAGTCTGGAAGTGA-3
T4799 944-945 NN denotes ′
T4800 945-946 , denotes ,
T4801 947-957 NN denotes F2-reverse
T4802 957-958 : denotes :
T4803 959-960 CD denotes 5
T4804 960-961 SYM denotes ′
T4805 961-962 : denotes -
T4806 962-987 NN denotes CCAGGACCTCAGGAGGCCAGTCA-3
T4807 987-988 NN denotes ′
T4808 989-990 -LRB- denotes (
T4809 990-993 CD denotes 474
T4810 994-996 NN denotes bp
T4811 996-997 -RRB- denotes )
T4812 997-998 . denotes .
T4813 999-1002 DT denotes The
T4814 1003-1006 NNP denotes PCR
T4815 1007-1017 NNS denotes conditions
T4816 1018-1022 VBD denotes were
T4817 1023-1032 VBN denotes described
T4818 1033-1042 RB denotes elsewhere
T4819 1043-1044 -LRB- denotes (
T4820 1044-1045 LS denotes 3
T4821 1045-1046 -RRB- denotes )
T4822 1046-1047 . denotes .
T4823 1048-1051 NNP denotes PCR
T4824 1052-1055 VBD denotes was
T4825 1056-1065 VBN denotes performed
T4826 1066-1070 IN denotes with
T4827 1071-1073 DT denotes an
T4828 1074-1083 NN denotes annealing
T4829 1084-1095 NN denotes temperature
T4830 1096-1098 IN denotes of
T4831 1099-1101 CD denotes 62
T4832 1101-1102 CD denotes °
T4833 1102-1103 NNP denotes C
T4834 1104-1107 CC denotes and
T4835 1108-1110 CD denotes 35
T4836 1111-1117 NNS denotes cycles
T4837 1117-1118 . denotes .
T4838 1119-1123 WRB denotes When
T4839 1124-1127 NNP denotes DNA
T4840 1128-1131 VBD denotes was
T4841 1132-1142 VBN denotes methylated
T4842 1143-1145 IN denotes at
T4843 1146-1154 JJ denotes specific
T4844 1155-1160 NNS denotes sites
T4845 1160-1161 , denotes ,
T4846 1162-1165 DT denotes the
T4847 1166-1175 JJ denotes sensitive
T4848 1176-1183 NNS denotes enzymes
T4849 1184-1188 VBD denotes were
T4850 1189-1192 RB denotes not
T4851 1193-1197 JJ denotes able
T4852 1198-1200 TO denotes to
T4853 1201-1207 VB denotes digest
T4854 1208-1211 DT denotes the
T4855 1212-1215 NN denotes DNA
T4856 1216-1219 CC denotes and
T4857 1220-1233 NN denotes amplification
T4858 1234-1238 VBD denotes took
T4859 1239-1244 NN denotes place
T4860 1244-1245 : denotes ;
T4861 1246-1248 IN denotes in
T4862 1249-1253 NN denotes case
T4863 1254-1256 IN denotes of
T4864 1257-1259 DT denotes no
T4865 1260-1271 NN denotes methylation
T4866 1271-1272 , denotes ,
T4867 1273-1276 NNP denotes DNA
T4868 1277-1280 VBD denotes was
T4869 1281-1289 VBN denotes digested
T4870 1290-1293 CC denotes and
T4871 1294-1296 DT denotes no
T4872 1297-1304 NN denotes product
T4873 1305-1308 VBD denotes was
T4874 1309-1318 VBN denotes generated
T4875 1318-1319 . denotes .
T4876 1320-1323 DT denotes The
T4877 1324-1327 NNP denotes PCR
T4878 1328-1336 NNS denotes products
T4879 1337-1341 VBD denotes were
T4880 1342-1357 VBN denotes electrophoresed
T4881 1358-1360 IN denotes on
T4882 1361-1362 DT denotes a
T4883 1363-1364 CD denotes 3
T4884 1364-1365 NN denotes %
T4885 1366-1373 JJ denotes agarose
T4886 1374-1377 NN denotes gel
T4887 1377-1378 , denotes ,
T4888 1379-1383 VBD denotes were
T4889 1384-1391 VBN denotes stained
T4890 1392-1396 IN denotes with
T4891 1397-1405 NN denotes ethidium
T4892 1406-1413 NN denotes bromide
T4893 1414-1417 CC denotes and
T4894 1418-1430 VBD denotes photographed
T4895 1430-1431 . denotes .
T4896 1432-1435 NNP denotes PCR
T4897 1436-1444 NNS denotes products
T4898 1445-1449 VBD denotes were
T4899 1450-1458 VBN denotes verified
T4900 1459-1461 IN denotes by
T4901 1462-1471 JJ denotes automated
T4902 1472-1482 NN denotes sequencing
T4903 1482-1483 . denotes .
R3780 T4626 T4628 amod Methylation-specific,DNA
R3781 T4627 T4628 amod restriction-PCR-assay,DNA
R3782 T4628 T4630 nsubjpass DNA,extracted
R3783 T4629 T4630 auxpass was,extracted
R3784 T4630 T4630 ROOT extracted,extracted
R3785 T4631 T4630 prep with,extracted
R3786 T4632 T4634 det a,kit
R3787 T4633 T4634 amod commercial,kit
R3788 T4634 T4631 pobj kit,with
R3789 T4635 T4634 punct (,kit
R3790 T4636 T4634 appos Qiagen,kit
R3791 T4637 T4634 punct ),kit
R3792 T4638 T4639 mark as,recommended
R3793 T4639 T4630 advcl recommended,extracted
R3794 T4640 T4630 punct .,extracted
R3795 T4641 T4649 mark Since,inhibited
R3796 T4642 T4644 det the,ability
R3797 T4643 T4644 compound restriction,ability
R3798 T4644 T4649 nsubjpass ability,inhibited
R3799 T4645 T4644 prep of,ability
R3800 T4646 T4647 amod several,endonucleases
R3801 T4647 T4645 pobj endonucleases,of
R3802 T4648 T4649 auxpass is,inhibited
R3803 T4649 T4658 advcl inhibited,used
R3804 T4650 T4649 agent by,inhibited
R3805 T4651 T4650 pobj methylation,by
R3806 T4652 T4651 prep of,methylation
R3807 T4653 T4655 poss their,sequence
R3808 T4654 T4655 compound target,sequence
R3809 T4655 T4652 pobj sequence,of
R3810 T4656 T4658 punct ",",used
R3811 T4657 T4658 nsubj we,used
R3812 T4658 T4658 ROOT used,used
R3813 T4659 T4660 amod methylation-sensitive,enzymes
R3814 T4660 T4658 dobj enzymes,used
R3815 T4661 T4660 appos HpaII,enzymes
R3816 T4662 T4661 cc and,HpaII
R3817 T4663 T4664 compound HaeII-isochizomer,Bsp143II
R3818 T4664 T4661 conj Bsp143II,HpaII
R3819 T4665 T4664 cc and,Bsp143II
R3820 T4666 T4664 conj Bsh1236I,Bsp143II
R3821 T4667 T4669 punct (,Fermentas
R3822 T4668 T4669 compound MBI,Fermentas
R3823 T4669 T4664 conj Fermentas,Bsp143II
R3824 T4670 T4669 punct ",",Fermentas
R3825 T4671 T4672 compound St,Leon-Rot
R3826 T4672 T4669 conj Leon-Rot,Fermentas
R3827 T4673 T4672 punct ",",Leon-Rot
R3828 T4674 T4672 appos Germany,Leon-Rot
R3829 T4675 T4672 punct ),Leon-Rot
R3830 T4676 T4677 punct (,"20,24"
R3831 T4677 T4672 appos "20,24",Leon-Rot
R3832 T4678 T4677 punct ),"20,24"
R3833 T4679 T4658 punct .,used
R3834 T4680 T4701 prep As,used
R3835 T4681 T4680 pobj control,As
R3836 T4682 T4684 det the,enzyme
R3837 T4683 T4684 compound methylation-resistant,enzyme
R3838 T4684 T4701 nsubjpass enzyme,used
R3839 T4685 T4684 appos MspI,enzyme
R3840 T4686 T4685 cc and,MspI
R3841 T4687 T4688 det an,enzyme
R3842 T4688 T4685 conj enzyme,MspI
R3843 T4689 T4688 prep with,enzyme
R3844 T4690 T4692 det no,site
R3845 T4691 T4692 compound recognition,site
R3846 T4692 T4689 pobj site,with
R3847 T4693 T4692 prep in,site
R3848 T4694 T4696 det the,promoter
R3849 T4695 T4696 compound target,promoter
R3850 T4696 T4693 pobj promoter,in
R3851 T4697 T4696 punct ",",promoter
R3852 T4698 T4696 appos EcoRI,promoter
R3853 T4699 T4701 punct ",",used
R3854 T4700 T4701 auxpass were,used
R3855 T4701 T4701 ROOT used,used
R3856 T4702 T4701 punct .,used
R3857 T4703 T4709 nsubjpass DNA,digested
R3858 T4704 T4706 punct (,µg
R3859 T4705 T4706 nummod 0.8,µg
R3860 T4706 T4703 appos µg,DNA
R3861 T4707 T4706 punct ),µg
R3862 T4708 T4709 auxpass was,digested
R3863 T4709 T4709 ROOT digested,digested
R3864 T4710 T4709 agent by,digested
R3865 T4711 T4715 nummod 40,enzyme
R3866 T4712 T4715 nmod U,enzyme
R3867 T4713 T4715 det the,enzyme
R3868 T4714 T4715 amod respective,enzyme
R3869 T4715 T4710 pobj enzyme,by
R3870 T4716 T4709 prep for,digested
R3871 T4717 T4718 nummod 6,h
R3872 T4718 T4716 pobj h,for
R3873 T4719 T4718 cc and,h
R3874 T4720 T4718 punct ",",h
R3875 T4721 T4722 aux to,ensure
R3876 T4722 T4709 advcl ensure,digested
R3877 T4723 T4724 amod complete,cleavage
R3878 T4724 T4722 dobj cleavage,ensure
R3879 T4725 T4724 punct ",",cleavage
R3880 T4726 T4728 amod additional,U
R3881 T4727 T4728 nummod 20,U
R3882 T4728 T4724 appos U,cleavage
R3883 T4729 T4722 prep for,ensure
R3884 T4730 T4731 nummod 16,h.
R3885 T4731 T4729 pobj h.,for
R3886 T4732 T4739 advmod Thereafter,used
R3887 T4733 T4734 nummod 100,ng
R3888 T4734 T4739 nsubjpass ng,used
R3889 T4735 T4734 prep of,ng
R3890 T4736 T4737 amod digested,DNA
R3891 T4737 T4735 pobj DNA,of
R3892 T4738 T4739 auxpass was,used
R3893 T4739 T4739 ROOT used,used
R3894 T4740 T4739 prep to,used
R3895 T4741 T4743 det a,amplification
R3896 T4742 T4743 compound PCR,amplification
R3897 T4743 T4740 pobj amplification,to
R3898 T4744 T4743 prep of,amplification
R3899 T4745 T4746 nummod two,fragments
R3900 T4746 T4744 pobj fragments,of
R3901 T4747 T4746 punct (,fragments
R3902 T4748 T4746 appos F1,fragments
R3903 T4749 T4748 cc and,F1
R3904 T4750 T4748 conj F2,F1
R3905 T4751 T4746 punct ),fragments
R3906 T4752 T4743 acl spanning,amplification
R3907 T4753 T4752 dobj part,spanning
R3908 T4754 T4753 prep of,part
R3909 T4755 T4757 det the,promoter
R3910 T4756 T4757 compound IRF-4,promoter
R3911 T4757 T4754 pobj promoter,of
R3912 T4758 T4757 punct (,promoter
R3913 T4759 T4757 appos 30,promoter
R3914 T4760 T4757 punct ),promoter
R3915 T4761 T4763 punct (,U52683
R3916 T4762 T4763 compound GenBank,U52683
R3917 T4763 T4743 appos U52683,amplification
R3918 T4764 T4765 punct ;,see
R3919 T4765 T4765 ROOT see,see
R3920 T4766 T4767 compound Figure,3A
R3921 T4767 T4765 dobj 3A,see
R3922 T4768 T4765 punct ),see
R3923 T4769 T4765 punct .,see
R3924 T4770 T4771 det The,sequences
R3925 T4771 T4775 nsubj sequences,were
R3926 T4772 T4771 prep of,sequences
R3927 T4773 T4774 det the,primers
R3928 T4774 T4772 pobj primers,of
R3929 T4775 T4775 ROOT were,were
R3930 T4776 T4775 acomp F1-forward,were
R3931 T4777 T4775 punct :,were
R3932 T4778 T4782 nummod 5,′
R3933 T4779 T4781 punct ′,TTGAGATGGAGTCTTGCTCTGT-3
R3934 T4780 T4781 punct -,TTGAGATGGAGTCTTGCTCTGT-3
R3935 T4781 T4782 compound TTGAGATGGAGTCTTGCTCTGT-3,′
R3936 T4782 T4793 npadvmod ′,F2-forward
R3937 T4783 T4784 punct ",",F1-reverse
R3938 T4784 T4793 nmod F1-reverse,F2-forward
R3939 T4785 T4784 punct :,F1-reverse
R3940 T4786 T4787 compound ATCACTTCCAGACTTCAGTTCACCT-3,′
R3941 T4787 T4784 appos ′,F1-reverse
R3942 T4788 T4790 punct (,bp
R3943 T4789 T4790 nummod 341,bp
R3944 T4790 T4787 appos bp,′
R3945 T4791 T4787 punct ),′
R3946 T4792 T4793 punct ;,F2-forward
R3947 T4793 T4775 dep F2-forward,were
R3948 T4794 T4793 punct :,F2-forward
R3949 T4795 T4799 nummod 5,′
R3950 T4796 T4798 punct ′,AAGGTGAACTGAAGTCTGGAAGTGA-3
R3951 T4797 T4798 punct -,AAGGTGAACTGAAGTCTGGAAGTGA-3
R3952 T4798 T4799 compound AAGGTGAACTGAAGTCTGGAAGTGA-3,′
R3953 T4799 T4793 appos ′,F2-forward
R3954 T4800 T4799 punct ",",′
R3955 T4801 T4799 conj F2-reverse,′
R3956 T4802 T4801 punct :,F2-reverse
R3957 T4803 T4807 nummod 5,′
R3958 T4804 T4806 punct ′,CCAGGACCTCAGGAGGCCAGTCA-3
R3959 T4805 T4806 punct -,CCAGGACCTCAGGAGGCCAGTCA-3
R3960 T4806 T4807 compound CCAGGACCTCAGGAGGCCAGTCA-3,′
R3961 T4807 T4801 appos ′,F2-reverse
R3962 T4808 T4810 punct (,bp
R3963 T4809 T4810 nummod 474,bp
R3964 T4810 T4807 appos bp,′
R3965 T4811 T4807 punct ),′
R3966 T4812 T4775 punct .,were
R3967 T4813 T4815 det The,conditions
R3968 T4814 T4815 compound PCR,conditions
R3969 T4815 T4817 nsubjpass conditions,described
R3970 T4816 T4817 auxpass were,described
R3971 T4817 T4817 ROOT described,described
R3972 T4818 T4817 advmod elsewhere,described
R3973 T4819 T4820 punct (,3
R3974 T4820 T4817 parataxis 3,described
R3975 T4821 T4820 punct ),3
R3976 T4822 T4817 punct .,described
R3977 T4823 T4825 nsubjpass PCR,performed
R3978 T4824 T4825 auxpass was,performed
R3979 T4825 T4825 ROOT performed,performed
R3980 T4826 T4825 prep with,performed
R3981 T4827 T4829 det an,temperature
R3982 T4828 T4829 compound annealing,temperature
R3983 T4829 T4826 pobj temperature,with
R3984 T4830 T4829 prep of,temperature
R3985 T4831 T4833 nummod 62,C
R3986 T4832 T4833 nummod °,C
R3987 T4833 T4830 pobj C,of
R3988 T4834 T4829 cc and,temperature
R3989 T4835 T4836 nummod 35,cycles
R3990 T4836 T4829 conj cycles,temperature
R3991 T4837 T4825 punct .,performed
R3992 T4838 T4841 advmod When,methylated
R3993 T4839 T4841 nsubjpass DNA,methylated
R3994 T4840 T4841 auxpass was,methylated
R3995 T4841 T4849 advcl methylated,were
R3996 T4842 T4841 prep at,methylated
R3997 T4843 T4844 amod specific,sites
R3998 T4844 T4842 pobj sites,at
R3999 T4845 T4849 punct ",",were
R4000 T4846 T4848 det the,enzymes
R4001 T4847 T4848 amod sensitive,enzymes
R4002 T4848 T4849 nsubj enzymes,were
R4003 T4849 T4869 ccomp were,digested
R4004 T4850 T4849 neg not,were
R4005 T4851 T4849 acomp able,were
R4006 T4852 T4853 aux to,digest
R4007 T4853 T4851 xcomp digest,able
R4008 T4854 T4855 det the,DNA
R4009 T4855 T4853 dobj DNA,digest
R4010 T4856 T4855 cc and,DNA
R4011 T4857 T4855 conj amplification,DNA
R4012 T4858 T4849 conj took,were
R4013 T4859 T4858 dobj place,took
R4014 T4860 T4869 punct ;,digested
R4015 T4861 T4869 prep in,digested
R4016 T4862 T4861 pobj case,in
R4017 T4863 T4862 prep of,case
R4018 T4864 T4865 det no,methylation
R4019 T4865 T4863 pobj methylation,of
R4020 T4866 T4869 punct ",",digested
R4021 T4867 T4869 nsubjpass DNA,digested
R4022 T4868 T4869 auxpass was,digested
R4023 T4869 T4869 ROOT digested,digested
R4024 T4870 T4869 cc and,digested
R4025 T4871 T4872 det no,product
R4026 T4872 T4874 nsubjpass product,generated
R4027 T4873 T4874 auxpass was,generated
R4028 T4874 T4869 conj generated,digested
R4029 T4875 T4874 punct .,generated
R4030 T4876 T4878 det The,products
R4031 T4877 T4878 compound PCR,products
R4032 T4878 T4880 nsubjpass products,electrophoresed
R4033 T4879 T4880 auxpass were,electrophoresed
R4034 T4880 T4880 ROOT electrophoresed,electrophoresed
R4035 T4881 T4880 prep on,electrophoresed
R4036 T4882 T4886 det a,gel
R4037 T4883 T4884 nummod 3,%
R4038 T4884 T4886 nmod %,gel
R4039 T4885 T4886 amod agarose,gel
R4040 T4886 T4881 pobj gel,on
R4041 T4887 T4889 punct ",",stained
R4042 T4888 T4889 auxpass were,stained
R4043 T4889 T4880 conj stained,electrophoresed
R4044 T4890 T4889 prep with,stained
R4045 T4891 T4892 compound ethidium,bromide
R4046 T4892 T4890 pobj bromide,with
R4047 T4893 T4889 cc and,stained
R4048 T4894 T4889 conj photographed,stained
R4049 T4895 T4889 punct .,stained
R4050 T4896 T4897 compound PCR,products
R4051 T4897 T4899 nsubjpass products,verified
R4052 T4898 T4899 auxpass were,verified
R4053 T4899 T4899 ROOT verified,verified
R4054 T4900 T4899 agent by,verified
R4055 T4901 T4902 amod automated,sequencing
R4056 T4902 T4900 pobj sequencing,by
R4057 T4903 T4899 punct .,verified

GO-BP

Id Subject Object Predicate Lexical cue
T4365 0-11 http://purl.obolibrary.org/obo/GO_0032259 denotes Methylation
T4366 179-190 http://purl.obolibrary.org/obo/GO_0032259 denotes methylation
T4367 225-236 http://purl.obolibrary.org/obo/GO_0032259 denotes methylation
T4368 367-378 http://purl.obolibrary.org/obo/GO_0032259 denotes methylation
T4369 1260-1271 http://purl.obolibrary.org/obo/GO_0032259 denotes methylation
T4370 1212-1233 http://purl.obolibrary.org/obo/GO_0006277 denotes DNA and amplification

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T4923 255-260 Protein denotes HpaII
T4924 265-291 Protein denotes HaeII-isochizomer Bsp143II
T4925 296-304 Protein denotes Bsh1236I
T4926 306-309 Protein denotes MBI
T4927 464-469 Protein denotes EcoRI
T4928 722-736 Protein denotes IRF-4 promoter

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T4349 722-727 Protein denotes IRF-4

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T4624 722-727 Q15306 denotes IRF-4
T4625 1193-1197 P00519 denotes able

test2

Id Subject Object Predicate Lexical cue
T4348 722-727 Protein denotes IRF-4