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PMC:1310901 / 6810-8716 JSONTXT

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bionlp-st-ge-2016-reference-eval

Id Subject Object Predicate Lexical cue
T4319 41-56 Transcription denotes transcriptional
T4320 463-473 Gene_expression denotes expression
T4321 609-619 Gene_expression denotes expression
T4322 242-256 Transcription denotes RNA expression
T4323 242-256 Transcription denotes RNA expression
T4324 463-473 Gene_expression denotes expression
T4325 463-473 Gene_expression denotes expression
T4326 463-473 Gene_expression denotes expression
T4327 463-473 Gene_expression denotes expression
T4328 463-473 Gene_expression denotes expression
T4329 463-473 Gene_expression denotes expression
T4330 35-40 Protein denotes IRF-4
T4331 542-546 Protein denotes MBD4
T4332 595-600 Protein denotes IRF-4
T4333 1805-1810 Protein denotes IRF-4
T4334 1811-1816 Protein denotes ICSAT
T4335 1818-1822 Protein denotes M-17
T4336 1882-1889 Protein denotes β-actin
T4337 270-275 Protein denotes IRF-4
T4338 299-306 Protein denotes β-actin
T4339 499-504 Protein denotes DNMT1
T4340 505-511 Protein denotes DNMT3A
T4341 513-519 Protein denotes DNMT3B
T4342 521-525 Protein denotes MeCP
T4343 527-531 Protein denotes MBD1
T4344 533-537 Protein denotes MBD2
T4345 486-490 Protein denotes DNMT
T4346 494-497 Protein denotes MBP
R3510 T4330 T4319 themeOf IRF-4,transcriptional
R3511 T4331 T4320 themeOf MBD4,expression
R3512 T4332 T4321 themeOf IRF-4,expression
R3513 T4334 T4333 equivalentTo ICSAT,IRF-4
R3514 T4335 T4333 equivalentTo M-17,IRF-4
R3515 T4337 T4322 themeOf IRF-4,RNA expression
R3516 T4338 T4323 themeOf β-actin,RNA expression
R3517 T4339 T4324 themeOf DNMT1,expression
R3518 T4340 T4325 themeOf DNMT3A,expression
R3519 T4341 T4326 themeOf DNMT3B,expression
R3520 T4342 T4327 themeOf MeCP,expression
R3521 T4343 T4328 themeOf MBD1,expression
R3522 T4344 T4329 themeOf MBD2,expression

events-check-again

Id Subject Object Predicate Lexical cue Speculation
T4269 35-40 Protein denotes IRF-4
T4270 41-56 Transcription denotes transcriptional
T4271 242-256 Transcription denotes RNA expression
T4272 242-256 Transcription denotes RNA expression
T4273 270-275 Protein denotes IRF-4
T4274 299-306 Protein denotes β-actin
T4275 463-473 Gene_expression denotes expression true
T4276 463-473 Gene_expression denotes expression true
T4277 463-473 Gene_expression denotes expression true
T4278 463-473 Gene_expression denotes expression true
T4279 463-473 Gene_expression denotes expression true
T4280 463-473 Gene_expression denotes expression true
T4281 463-473 Gene_expression denotes expression true
T4282 499-504 Protein denotes DNMT1
T4283 505-511 Protein denotes DNMT3A
T4284 513-519 Protein denotes DNMT3B
T4285 521-525 Protein denotes MeCP
T4286 527-531 Protein denotes MBD1
T4287 533-537 Protein denotes MBD2
T4288 542-546 Protein denotes MBD4
T4289 595-600 Protein denotes IRF-4
T4290 609-619 Gene_expression denotes expression true
T4291 1805-1810 Protein denotes IRF-4
T4292 1811-1816 Protein denotes ICSAT
T4293 1818-1822 Protein denotes M-17
T4294 1882-1889 Protein denotes β-actin
R3488 T4269 T4270 themeOf IRF-4,transcriptional
R3489 T4273 T4271 themeOf IRF-4,RNA expression
R3490 T4274 T4272 themeOf β-actin,RNA expression
R3491 T4282 T4276 themeOf DNMT1,expression
R3492 T4283 T4277 themeOf DNMT3A,expression
R3493 T4284 T4278 themeOf DNMT3B,expression
R3494 T4285 T4279 themeOf MeCP,expression
R3495 T4286 T4280 themeOf MBD1,expression
R3496 T4287 T4281 themeOf MBD2,expression
R3497 T4288 T4275 themeOf MBD4,expression
R3498 T4289 T4290 themeOf IRF-4,expression
R3499 T4292 T4291 equivalentTo ICSAT,IRF-4
R3500 T4293 T4291 equivalentTo M-17,IRF-4

GO-CC

Id Subject Object Predicate Lexical cue
T4186 1580-1588 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T4187 1779-1789 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T4188 1510-1518 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T4189 1580-1588 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T4190 1779-1789 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T4180 87-92 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4181 759-764 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4182 1217-1225 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T4183 1280-1289 http://purl.obolibrary.org/obo/GO_0016020 denotes Membranes
T4184 1662-1671 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T4185 1510-1518 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody

sentences

Id Subject Object Predicate Lexical cue
T3408 0-19 Sentence denotes Expression analysis
T3409 20-154 Sentence denotes To analyze the IRF-4 transcriptional level, RNA was extracted from cells using the commercial RNAzol-kit (Paesel, Frankfurt, Germany).
T3410 155-241 Sentence denotes An aliquot of 1 μg total RNA was used for cDNA synthesis as described previously (27).
T3411 242-379 Sentence denotes RNA expression analysis for IRF-4 and the reference gene β-actin was carried out by semi-quantitative PCR as described previously (3,27).
T3412 380-431 Sentence denotes PCR products were verified by automated sequencing.
T3413 432-578 Sentence denotes PCR primers and conditions for expression analysis of DNMT or MBP (DNMT1 DNMT3A, DNMT3B, MeCP, MBD1, MBD2 and MBD4) were published elsewhere (28).
T3414 579-696 Sentence denotes For analysis of IRF-4 protein expression, a standard immunoblotting assay was performed as described previously (29).
T3415 697-987 Sentence denotes Briefly, protein lysates were generated by incubating 1 × 106 cells in 100 µl RIPA buffer (1% NP-40, 0.5% sodiumdesoxycholate, 0.1% SDS, 100 µg/ml phenylmethylsulfonyl fluoride, 10 µl/ml protease-inhibitory-mix, 1 µmol/ml sodiumorthovanadate in phosphate-buffered saline) for 30 min on ice.
T3416 988-1118 Sentence denotes After centrifugation, protein concentration of the supernatant was determined by BCA-method (Pierce, Rockford, IL) as recommended.
T3417 1119-1279 Sentence denotes Protein lysates (70–100 µg) were electrophoresed on polyacrylamide gels and transferred to a PVDF-membrane (Immobilon P, 0.45 µm; Millipore, Eschborn, Germany).
T3418 1280-1657 Sentence denotes Membranes were blocked with 2.5% blocking reagent (Boehringer Mannheim, Germany) in TBST buffer (4.44 g/l Tris–HCL, 2.65 g/l TrisOH, 8.07 g/l NaCl, 0.2 g/l KCl and 500 µl/l Tween-20 in H2O) and subsequently incubated with primary antibody as indicated and horseradish peroxidase-conjugated secondary antibody, anti-mouse or anti-goat IgG (DAKO, Hamburg, Germany), respectively.
T3419 1658-1766 Sentence denotes The membranes were then developed with an ECL detection kit (Amersham Pharmacia Biotech, Freiburg, Germany).
T3420 1767-1906 Sentence denotes The primary antibodies were goat anti-IRF-4/ICSAT (M-17) (Santa Cruz Biotechnology, Santa Cruz, CA) and mouse anti-β-actin (AC-74) (Sigma).
T51 0-19 Sentence denotes Expression analysis
T52 20-154 Sentence denotes To analyze the IRF-4 transcriptional level, RNA was extracted from cells using the commercial RNAzol-kit (Paesel, Frankfurt, Germany).
T53 155-241 Sentence denotes An aliquot of 1 μg total RNA was used for cDNA synthesis as described previously (27).
T54 242-379 Sentence denotes RNA expression analysis for IRF-4 and the reference gene β-actin was carried out by semi-quantitative PCR as described previously (3,27).
T55 380-431 Sentence denotes PCR products were verified by automated sequencing.
T56 432-578 Sentence denotes PCR primers and conditions for expression analysis of DNMT or MBP (DNMT1 DNMT3A, DNMT3B, MeCP, MBD1, MBD2 and MBD4) were published elsewhere (28).
T57 579-696 Sentence denotes For analysis of IRF-4 protein expression, a standard immunoblotting assay was performed as described previously (29).
T58 697-987 Sentence denotes Briefly, protein lysates were generated by incubating 1 × 106 cells in 100 µl RIPA buffer (1% NP-40, 0.5% sodiumdesoxycholate, 0.1% SDS, 100 µg/ml phenylmethylsulfonyl fluoride, 10 µl/ml protease-inhibitory-mix, 1 µmol/ml sodiumorthovanadate in phosphate-buffered saline) for 30 min on ice.
T59 988-1118 Sentence denotes After centrifugation, protein concentration of the supernatant was determined by BCA-method (Pierce, Rockford, IL) as recommended.
T60 1119-1279 Sentence denotes Protein lysates (70–100 µg) were electrophoresed on polyacrylamide gels and transferred to a PVDF-membrane (Immobilon P, 0.45 µm; Millipore, Eschborn, Germany).
T61 1280-1657 Sentence denotes Membranes were blocked with 2.5% blocking reagent (Boehringer Mannheim, Germany) in TBST buffer (4.44 g/l Tris–HCL, 2.65 g/l TrisOH, 8.07 g/l NaCl, 0.2 g/l KCl and 500 µl/l Tween-20 in H2O) and subsequently incubated with primary antibody as indicated and horseradish peroxidase-conjugated secondary antibody, anti-mouse or anti-goat IgG (DAKO, Hamburg, Germany), respectively.
T62 1658-1766 Sentence denotes The membranes were then developed with an ECL detection kit (Amersham Pharmacia Biotech, Freiburg, Germany).
T63 1767-1906 Sentence denotes The primary antibodies were goat anti-IRF-4/ICSAT (M-17) (Santa Cruz Biotechnology, Santa Cruz, CA) and mouse anti-β-actin (AC-74) (Sigma).

2_test

Id Subject Object Predicate Lexical cue
16396836-9414265-76538181 237-239 9414265 denotes 27
16396836-11013272-76538182 373-374 11013272 denotes 3
16396836-9414265-76538183 375-377 9414265 denotes 27
16396836-12101420-76538184 574-576 12101420 denotes 28
16396836-14656881-76538185 692-694 14656881 denotes 29

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T3450 0-10 NN denotes Expression
T3451 11-19 NN denotes analysis
T3452 20-22 TO denotes To
T3453 23-30 VB denotes analyze
T3454 31-34 DT denotes the
T3455 35-40 NN denotes IRF-4
T3456 41-56 JJ denotes transcriptional
T3457 57-62 NN denotes level
T3458 62-63 -COMMA- denotes ,
T3459 64-67 NN denotes RNA
T3460 68-71 VB denotes was
T3461 72-81 VB denotes extracted
T3462 82-86 IN denotes from
T3463 87-92 NN denotes cells
T3464 93-98 VB denotes using
T3465 99-102 DT denotes the
T3466 103-113 JJ denotes commercial
T3467 114-124 NN denotes RNAzol-kit
T3468 125-126 -LRB- denotes (
T3469 126-132 NNP denotes Paesel
T3470 132-133 -COMMA- denotes ,
T3471 134-143 NNP denotes Frankfurt
T3472 143-144 -COMMA- denotes ,
T3473 145-152 NNP denotes Germany
T3474 152-153 -RRB- denotes )
T3475 155-157 DT denotes An
T3476 158-165 NN denotes aliquot
T3477 166-168 IN denotes of
T3478 169-170 CD denotes 1
T3479 171-173 NN denotes μg
T3480 174-179 JJ denotes total
T3481 180-183 NN denotes RNA
T3482 184-187 VB denotes was
T3483 188-192 VB denotes used
T3484 193-196 IN denotes for
T3485 197-201 NN denotes cDNA
T3486 202-211 NN denotes synthesis
T3487 212-214 IN denotes as
T3488 215-224 VB denotes described
T3489 225-235 RB denotes previously
T3490 236-237 -LRB- denotes (
T3491 237-239 CD denotes 27
T3492 239-240 -RRB- denotes )
T3493 242-245 NN denotes RNA
T3494 246-256 NN denotes expression
T3495 257-265 NN denotes analysis
T3496 266-269 IN denotes for
T3497 270-275 NN denotes IRF-4
T3498 276-279 CC denotes and
T3499 280-283 DT denotes the
T3500 284-293 NN denotes reference
T3501 294-298 NN denotes gene
T3502 299-306 NN denotes β-actin
T3503 307-310 VB denotes was
T3504 311-318 VB denotes carried
T3505 319-322 RP denotes out
T3506 323-325 IN denotes by
T3507 326-343 JJ denotes semi-quantitative
T3508 344-347 NN denotes PCR
T3509 348-350 IN denotes as
T3510 351-360 VB denotes described
T3511 361-371 RB denotes previously
T3512 372-373 -LRB- denotes (
T3513 373-377 CD denotes 3,27
T3514 377-378 -RRB- denotes )
T3515 380-383 NN denotes PCR
T3516 384-392 NN denotes products
T3517 393-397 VB denotes were
T3518 398-406 VB denotes verified
T3519 407-409 IN denotes by
T3520 410-419 VB denotes automated
T3521 420-430 NN denotes sequencing
T3522 432-435 NN denotes PCR
T3523 436-443 NN denotes primers
T3524 444-447 CC denotes and
T3525 448-458 NN denotes conditions
T3526 459-462 IN denotes for
T3527 463-473 NN denotes expression
T3528 474-482 NN denotes analysis
T3529 483-485 IN denotes of
T3530 486-490 NN denotes DNMT
T3531 491-493 CC denotes or
T3532 494-497 NN denotes MBP
T3533 498-499 -LRB- denotes (
T3534 499-504 NN denotes DNMT1
T3535 505-511 NN denotes DNMT3A
T3536 511-512 -COMMA- denotes ,
T3537 513-519 NN denotes DNMT3B
T3538 519-520 -COMMA- denotes ,
T3539 521-525 NN denotes MeCP
T3540 525-526 -COMMA- denotes ,
T3541 527-531 NN denotes MBD1
T3542 531-532 -COMMA- denotes ,
T3543 533-537 NN denotes MBD2
T3544 538-541 CC denotes and
T3545 542-546 NN denotes MBD4
T3546 546-547 -RRB- denotes )
T3547 548-552 VB denotes were
T3548 553-562 VB denotes published
T3549 563-572 RB denotes elsewhere
T3550 573-574 -LRB- denotes (
T3551 574-576 CD denotes 28
T3552 576-577 -RRB- denotes )
T3553 579-582 IN denotes For
T3554 583-591 NN denotes analysis
T3555 592-594 IN denotes of
T3556 595-600 NN denotes IRF-4
T3557 601-608 NN denotes protein
T3558 609-619 NN denotes expression
T3559 619-620 -COMMA- denotes ,
T3560 621-622 DT denotes a
T3561 623-631 JJ denotes standard
T3562 632-646 NN denotes immunoblotting
T3563 647-652 NN denotes assay
T3564 653-656 VB denotes was
T3565 657-666 VB denotes performed
T3566 667-669 IN denotes as
T3567 670-679 VB denotes described
T3568 680-690 RB denotes previously
T3569 691-692 -LRB- denotes (
T3570 692-694 CD denotes 29
T3571 694-695 -RRB- denotes )
T3572 697-704 RB denotes Briefly
T3573 704-705 -COMMA- denotes ,
T3574 706-713 NN denotes protein
T3575 714-721 NN denotes lysates
T3576 722-726 VB denotes were
T3577 727-736 VB denotes generated
T3578 737-739 IN denotes by
T3579 740-750 VB denotes incubating
T3580 751-752 CD denotes 1
T3581 753-754 NN denotes ×
T3582 755-758 CD denotes 106
T3583 759-764 NN denotes cells
T3584 765-767 IN denotes in
T3585 768-771 CD denotes 100
T3586 772-774 NN denotes µl
T3587 775-779 NN denotes RIPA
T3588 780-786 NN denotes buffer
T3589 787-788 -LRB- denotes (
T3590 788-789 CD denotes 1
T3591 789-790 NN denotes %
T3592 791-796 NN denotes NP-40
T3593 796-797 -COMMA- denotes ,
T3594 798-801 CD denotes 0.5
T3595 801-802 NN denotes %
T3596 803-822 NN denotes sodiumdesoxycholate
T3597 822-823 -COMMA- denotes ,
T3598 824-827 CD denotes 0.1
T3599 827-828 NN denotes %
T3600 829-832 NN denotes SDS
T3601 832-833 -COMMA- denotes ,
T3602 834-837 CD denotes 100
T3603 838-843 NN denotes µg/ml
T3604 844-864 NN denotes phenylmethylsulfonyl
T3605 865-873 NN denotes fluoride
T3606 873-874 -COMMA- denotes ,
T3607 875-877 CD denotes 10
T3608 878-883 NN denotes µl/ml
T3609 884-907 NN denotes protease-inhibitory-mix
T3610 907-908 -COMMA- denotes ,
T3611 909-910 CD denotes 1
T3612 911-918 NN denotes µmol/ml
T3613 919-938 NN denotes sodiumorthovanadate
T3614 939-941 IN denotes in
T3615 942-960 JJ denotes phosphate-buffered
T3616 961-967 NN denotes saline
T3617 967-968 -RRB- denotes )
T3618 969-972 IN denotes for
T3619 973-975 CD denotes 30
T3620 976-979 NN denotes min
T3621 980-982 IN denotes on
T3622 983-986 NN denotes ice
T3623 988-993 IN denotes After
T3624 994-1008 NN denotes centrifugation
T3625 1008-1009 -COMMA- denotes ,
T3626 1010-1017 NN denotes protein
T3627 1018-1031 NN denotes concentration
T3628 1032-1034 IN denotes of
T3629 1035-1038 DT denotes the
T3630 1039-1050 NN denotes supernatant
T3631 1051-1054 VB denotes was
T3632 1055-1065 VB denotes determined
T3633 1066-1068 IN denotes by
T3634 1069-1079 NN denotes BCA-method
T3635 1080-1081 -LRB- denotes (
T3636 1081-1087 NNP denotes Pierce
T3637 1087-1088 -COMMA- denotes ,
T3638 1089-1097 NNP denotes Rockford
T3639 1097-1098 -COMMA- denotes ,
T3640 1099-1101 NN denotes IL
T3641 1101-1102 -RRB- denotes )
T3642 1103-1105 IN denotes as
T3643 1106-1117 VB denotes recommended
T3644 1119-1126 NN denotes Protein
T3645 1127-1134 NN denotes lysates
T3646 1135-1136 -LRB- denotes (
T3647 1136-1142 CD denotes 70–100
T3648 1143-1145 NN denotes µg
T3649 1145-1146 -RRB- denotes )
T3650 1147-1151 VB denotes were
T3651 1152-1167 VB denotes electrophoresed
T3652 1168-1170 IN denotes on
T3653 1171-1185 NN denotes polyacrylamide
T3654 1186-1190 NN denotes gels
T3655 1191-1194 CC denotes and
T3656 1195-1206 VB denotes transferred
T3657 1207-1209 TO denotes to
T3658 1210-1211 DT denotes a
T3659 1212-1225 NN denotes PVDF-membrane
T3660 1226-1227 -LRB- denotes (
T3661 1227-1236 NNP denotes Immobilon
T3662 1237-1238 NNP denotes P
T3663 1238-1239 -COMMA- denotes ,
T3664 1240-1244 CD denotes 0.45
T3665 1245-1247 NN denotes µm
T3666 1247-1248 -COLON- denotes ;
T3667 1249-1258 NNP denotes Millipore
T3668 1258-1259 -COMMA- denotes ,
T3669 1260-1268 NNP denotes Eschborn
T3670 1268-1269 -COMMA- denotes ,
T3671 1270-1277 NNP denotes Germany
T3672 1277-1278 -RRB- denotes )
T3673 1280-1289 NN denotes Membranes
T3674 1290-1294 VB denotes were
T3675 1295-1302 VB denotes blocked
T3676 1303-1307 IN denotes with
T3677 1308-1311 CD denotes 2.5
T3678 1311-1312 NN denotes %
T3679 1313-1321 VB denotes blocking
T3680 1322-1329 NN denotes reagent
T3681 1330-1331 -LRB- denotes (
T3682 1331-1341 NNP denotes Boehringer
T3683 1342-1350 NNP denotes Mannheim
T3684 1350-1351 -COMMA- denotes ,
T3685 1352-1359 NNP denotes Germany
T3686 1359-1360 -RRB- denotes )
T3687 1361-1363 IN denotes in
T3688 1364-1368 NN denotes TBST
T3689 1369-1375 NN denotes buffer
T3690 1376-1377 -LRB- denotes (
T3691 1377-1381 CD denotes 4.44
T3692 1382-1385 NN denotes g/l
T3693 1386-1394 NN denotes Tris–HCL
T3694 1394-1395 -COMMA- denotes ,
T3695 1396-1400 CD denotes 2.65
T3696 1401-1404 NN denotes g/l
T3697 1405-1411 NN denotes TrisOH
T3698 1411-1412 -COMMA- denotes ,
T3699 1413-1417 CD denotes 8.07
T3700 1418-1421 NN denotes g/l
T3701 1422-1426 NN denotes NaCl
T3702 1426-1427 -COMMA- denotes ,
T3703 1428-1431 CD denotes 0.2
T3704 1432-1435 NN denotes g/l
T3705 1436-1439 NN denotes KCl
T3706 1440-1443 CC denotes and
T3707 1444-1447 CD denotes 500
T3708 1448-1452 VB denotes µl/l
T3709 1453-1461 CD denotes Tween-20
T3710 1462-1464 IN denotes in
T3711 1465-1468 NN denotes H2O
T3712 1468-1469 -RRB- denotes )
T3713 1470-1473 CC denotes and
T3714 1474-1486 RB denotes subsequently
T3715 1487-1496 VB denotes incubated
T3716 1497-1501 IN denotes with
T3717 1502-1509 JJ denotes primary
T3718 1510-1518 NN denotes antibody
T3719 1519-1521 IN denotes as
T3720 1522-1531 VB denotes indicated
T3721 1532-1535 CC denotes and
T3722 1536-1547 JJ denotes horseradish
T3723 1548-1569 JJ denotes peroxidase-conjugated
T3724 1570-1579 JJ denotes secondary
T3725 1580-1588 NN denotes antibody
T3726 1588-1589 -COMMA- denotes ,
T3727 1590-1600 JJ denotes anti-mouse
T3728 1601-1603 CC denotes or
T3729 1604-1613 JJ denotes anti-goat
T3730 1614-1617 NN denotes IgG
T3731 1618-1619 -LRB- denotes (
T3732 1619-1623 NNP denotes DAKO
T3733 1623-1624 -COMMA- denotes ,
T3734 1625-1632 NNP denotes Hamburg
T3735 1632-1633 -COMMA- denotes ,
T3736 1634-1641 NNP denotes Germany
T3737 1641-1642 -RRB- denotes )
T3738 1642-1643 -COMMA- denotes ,
T3739 1644-1656 RB denotes respectively
T3740 1658-1661 DT denotes The
T3741 1662-1671 NN denotes membranes
T3742 1672-1676 VB denotes were
T3743 1677-1681 RB denotes then
T3744 1682-1691 VB denotes developed
T3745 1692-1696 IN denotes with
T3746 1697-1699 DT denotes an
T3747 1700-1703 NN denotes ECL
T3748 1704-1713 NN denotes detection
T3749 1714-1717 NN denotes kit
T3750 1718-1719 -LRB- denotes (
T3751 1719-1727 NNP denotes Amersham
T3752 1728-1737 NNP denotes Pharmacia
T3753 1738-1745 NNP denotes Biotech
T3754 1745-1746 -COMMA- denotes ,
T3755 1747-1755 NNP denotes Freiburg
T3756 1755-1756 -COMMA- denotes ,
T3757 1757-1764 NNP denotes Germany
T3758 1764-1765 -RRB- denotes )
T3759 1767-1770 DT denotes The
T3760 1771-1778 JJ denotes primary
T3761 1779-1789 NN denotes antibodies
T3762 1790-1794 VB denotes were
T3763 1795-1799 NN denotes goat
T3764 1800-1816 NN denotes anti-IRF-4/ICSAT
T3765 1817-1818 -LRB- denotes (
T3766 1818-1822 NN denotes M-17
T3767 1822-1823 -RRB- denotes )
T3768 1824-1825 -LRB- denotes (
T3769 1825-1830 NNP denotes Santa
T3770 1831-1835 NNP denotes Cruz
T3771 1836-1849 NNP denotes Biotechnology
T3772 1849-1850 -COMMA- denotes ,
T3773 1851-1856 NNP denotes Santa
T3774 1857-1861 NNP denotes Cruz
T3775 1861-1862 -COMMA- denotes ,
T3776 1863-1865 NN denotes CA
T3777 1865-1866 -RRB- denotes )
T3778 1867-1870 CC denotes and
T3779 1871-1876 NN denotes mouse
T3780 1877-1889 JJ denotes anti-β-actin
T3781 1890-1891 -LRB- denotes (
T3782 1891-1896 NN denotes AC-74
T3783 1896-1897 -RRB- denotes )
T3784 1898-1899 -LRB- denotes (
T3785 1899-1904 NN denotes Sigma
T3786 1904-1905 -RRB- denotes )
R2749 T3451 T3450 arg1Of analysis,Expression
R2750 T3453 T3452 arg1Of analyze,To
R2751 T3457 T3453 arg2Of level,analyze
R2752 T3457 T3454 arg1Of level,the
R2753 T3457 T3455 arg1Of level,IRF-4
R2754 T3457 T3456 arg1Of level,transcriptional
R2755 T3459 T3453 arg1Of RNA,analyze
R2756 T3459 T3460 arg1Of RNA,was
R2757 T3459 T3461 arg2Of RNA,extracted
R2758 T3461 T3452 modOf extracted,To
R2759 T3461 T3458 arg1Of extracted,","
R2760 T3461 T3460 arg2Of extracted,was
R2761 T3461 T3462 arg1Of extracted,from
R2762 T3463 T3462 arg2Of cells,from
R2763 T3463 T3464 arg1Of cells,using
R2764 T3467 T3464 arg2Of RNAzol-kit,using
R2765 T3467 T3465 arg1Of RNAzol-kit,the
R2766 T3467 T3466 arg1Of RNAzol-kit,commercial
R2767 T3467 T3468 arg1Of RNAzol-kit,(
R2768 T3469 T3470 arg1Of Paesel,","
R2769 T3470 T3468 arg2Of ",",(
R2770 T3470 T3472 arg1Of ",",","
R2771 T3471 T3470 arg2Of Frankfurt,","
R2772 T3473 T3472 arg2Of Germany,","
R2773 T3474 T3468 arg3Of ),(
R2774 T3476 T3475 arg1Of aliquot,An
R2775 T3476 T3477 arg1Of aliquot,of
R2776 T3476 T3482 arg1Of aliquot,was
R2777 T3476 T3483 arg2Of aliquot,used
R2778 T3481 T3477 arg2Of RNA,of
R2779 T3481 T3478 arg1Of RNA,1
R2780 T3481 T3479 arg1Of RNA,μg
R2781 T3481 T3480 arg1Of RNA,total
R2782 T3483 T3482 arg2Of used,was
R2783 T3483 T3484 arg1Of used,for
R2784 T3486 T3484 arg2Of synthesis,for
R2785 T3486 T3485 arg1Of synthesis,cDNA
R2786 T3487 T3483 arg3Of as,used
R2787 T3488 T3487 arg2Of described,as
R2788 T3488 T3489 arg1Of described,previously
R2789 T3489 T3490 arg1Of previously,(
R2790 T3491 T3490 arg2Of 27,(
R2791 T3492 T3490 arg3Of ),(
R2792 T3495 T3493 arg1Of analysis,RNA
R2793 T3495 T3494 arg1Of analysis,expression
R2794 T3495 T3496 arg1Of analysis,for
R2795 T3495 T3498 arg1Of analysis,and
R2796 T3497 T3496 arg2Of IRF-4,for
R2797 T3498 T3503 arg1Of and,was
R2798 T3498 T3504 arg2Of and,carried
R2799 T3502 T3498 arg2Of β-actin,and
R2800 T3502 T3499 arg1Of β-actin,the
R2801 T3502 T3500 arg1Of β-actin,reference
R2802 T3502 T3501 arg1Of β-actin,gene
R2803 T3504 T3503 arg2Of carried,was
R2804 T3504 T3505 arg1Of carried,out
R2805 T3504 T3509 arg1Of carried,as
R2806 T3508 T3504 arg1Of PCR,carried
R2807 T3508 T3506 arg2Of PCR,by
R2808 T3508 T3507 arg1Of PCR,semi-quantitative
R2809 T3510 T3509 arg2Of described,as
R2810 T3510 T3511 arg1Of described,previously
R2811 T3511 T3512 arg1Of previously,(
R2812 T3513 T3512 arg2Of "3,27",(
R2813 T3514 T3512 arg3Of ),(
R2814 T3516 T3515 arg1Of products,PCR
R2815 T3516 T3517 arg1Of products,were
R2816 T3516 T3518 arg2Of products,verified
R2817 T3518 T3517 arg2Of verified,were
R2818 T3521 T3518 arg1Of sequencing,verified
R2819 T3521 T3519 arg2Of sequencing,by
R2820 T3521 T3520 arg2Of sequencing,automated
R2821 T3523 T3522 arg1Of primers,PCR
R2822 T3523 T3524 arg1Of primers,and
R2823 T3524 T3526 arg1Of and,for
R2824 T3524 T3547 arg1Of and,were
R2825 T3524 T3548 arg2Of and,published
R2826 T3525 T3524 arg2Of conditions,and
R2827 T3528 T3526 arg2Of analysis,for
R2828 T3528 T3527 arg1Of analysis,expression
R2829 T3528 T3529 arg1Of analysis,of
R2830 T3530 T3531 arg1Of DNMT,or
R2831 T3531 T3529 arg2Of or,of
R2832 T3531 T3533 arg1Of or,(
R2833 T3532 T3531 arg2Of MBP,or
R2834 T3535 T3534 arg1Of DNMT3A,DNMT1
R2835 T3535 T3536 arg1Of DNMT3A,","
R2836 T3536 T3538 arg1Of ",",","
R2837 T3537 T3536 arg2Of DNMT3B,","
R2838 T3538 T3540 arg1Of ",",","
R2839 T3539 T3538 arg2Of MeCP,","
R2840 T3540 T3542 arg1Of ",",","
R2841 T3541 T3540 arg2Of MBD1,","
R2842 T3542 T3544 arg1Of ",",and
R2843 T3543 T3542 arg2Of MBD2,","
R2844 T3544 T3533 arg2Of and,(
R2845 T3545 T3544 arg2Of MBD4,and
R2846 T3546 T3533 arg3Of ),(
R2847 T3548 T3547 arg2Of published,were
R2848 T3548 T3549 arg1Of published,elsewhere
R2849 T3549 T3550 arg1Of elsewhere,(
R2850 T3551 T3550 arg2Of 28,(
R2851 T3552 T3550 arg3Of ),(
R2852 T3554 T3553 arg2Of analysis,For
R2853 T3554 T3555 arg1Of analysis,of
R2854 T3558 T3555 arg2Of expression,of
R2855 T3558 T3556 arg1Of expression,IRF-4
R2856 T3558 T3557 arg1Of expression,protein
R2857 T3563 T3560 arg1Of assay,a
R2858 T3563 T3561 arg1Of assay,standard
R2859 T3563 T3562 arg1Of assay,immunoblotting
R2860 T3563 T3564 arg1Of assay,was
R2861 T3563 T3565 arg2Of assay,performed
R2862 T3565 T3553 arg1Of performed,For
R2863 T3565 T3559 arg1Of performed,","
R2864 T3565 T3564 arg2Of performed,was
R2865 T3565 T3566 arg1Of performed,as
R2866 T3567 T3566 arg2Of described,as
R2867 T3567 T3568 arg1Of described,previously
R2868 T3568 T3569 arg1Of previously,(
R2869 T3570 T3569 arg2Of 29,(
R2870 T3571 T3569 arg3Of ),(
R2871 T3575 T3574 arg1Of lysates,protein
R2872 T3575 T3576 arg1Of lysates,were
R2873 T3575 T3577 arg2Of lysates,generated
R2874 T3577 T3572 arg1Of generated,Briefly
R2875 T3577 T3573 arg1Of generated,","
R2876 T3577 T3576 arg2Of generated,were
R2877 T3577 T3578 arg1Of generated,by
R2878 T3579 T3578 arg2Of incubating,by
R2879 T3579 T3618 arg1Of incubating,for
R2880 T3583 T3579 arg2Of cells,incubating
R2881 T3583 T3580 arg1Of cells,1
R2882 T3583 T3581 arg1Of cells,×
R2883 T3583 T3582 arg1Of cells,106
R2884 T3583 T3584 arg1Of cells,in
R2885 T3583 T3589 arg1Of cells,(
R2886 T3588 T3584 arg2Of buffer,in
R2887 T3588 T3585 arg1Of buffer,100
R2888 T3588 T3586 arg1Of buffer,µl
R2889 T3588 T3587 arg1Of buffer,RIPA
R2890 T3590 T3591 arg1Of 1,%
R2891 T3592 T3590 arg1Of NP-40,1
R2892 T3592 T3593 arg1Of NP-40,","
R2893 T3593 T3597 arg1Of ",",","
R2894 T3594 T3595 arg1Of 0.5,%
R2895 T3596 T3593 arg2Of sodiumdesoxycholate,","
R2896 T3596 T3594 arg1Of sodiumdesoxycholate,0.5
R2897 T3597 T3601 arg1Of ",",","
R2898 T3598 T3599 arg1Of 0.1,%
R2899 T3600 T3597 arg2Of SDS,","
R2900 T3600 T3598 arg1Of SDS,0.1
R2901 T3601 T3606 arg1Of ",",","
R2902 T3605 T3601 arg2Of fluoride,","
R2903 T3605 T3602 arg1Of fluoride,100
R2904 T3605 T3603 arg1Of fluoride,µg/ml
R2905 T3605 T3604 arg1Of fluoride,phenylmethylsulfonyl
R2906 T3606 T3610 arg1Of ",",","
R2907 T3609 T3606 arg2Of protease-inhibitory-mix,","
R2908 T3609 T3607 arg1Of protease-inhibitory-mix,10
R2909 T3609 T3608 arg1Of protease-inhibitory-mix,µl/ml
R2910 T3610 T3589 arg2Of ",",(
R2911 T3613 T3610 arg2Of sodiumorthovanadate,","
R2912 T3613 T3611 arg1Of sodiumorthovanadate,1
R2913 T3613 T3612 arg1Of sodiumorthovanadate,µmol/ml
R2914 T3613 T3614 arg1Of sodiumorthovanadate,in
R2915 T3616 T3614 arg2Of saline,in
R2916 T3616 T3615 arg1Of saline,phosphate-buffered
R2917 T3617 T3589 arg3Of ),(
R2918 T3620 T3618 arg2Of min,for
R2919 T3620 T3619 arg1Of min,30
R2920 T3620 T3621 arg1Of min,on
R2921 T3622 T3621 arg2Of ice,on
R2922 T3624 T3623 arg2Of centrifugation,After
R2923 T3627 T3626 arg1Of concentration,protein
R2924 T3627 T3628 arg1Of concentration,of
R2925 T3627 T3631 arg1Of concentration,was
R2926 T3627 T3632 arg2Of concentration,determined
R2927 T3630 T3628 arg2Of supernatant,of
R2928 T3630 T3629 arg1Of supernatant,the
R2929 T3632 T3623 arg1Of determined,After
R2930 T3632 T3625 arg1Of determined,","
R2931 T3632 T3631 arg2Of determined,was
R2932 T3632 T3635 arg1Of determined,(
R2933 T3632 T3642 arg1Of determined,as
R2934 T3634 T3632 arg1Of BCA-method,determined
R2935 T3634 T3633 arg2Of BCA-method,by
R2936 T3636 T3637 arg1Of Pierce,","
R2937 T3637 T3635 arg2Of ",",(
R2938 T3637 T3639 arg1Of ",",","
R2939 T3638 T3637 arg2Of Rockford,","
R2940 T3640 T3639 arg2Of IL,","
R2941 T3641 T3635 arg3Of ),(
R2942 T3643 T3642 arg2Of recommended,as
R2943 T3645 T3644 arg1Of lysates,Protein
R2944 T3645 T3646 arg1Of lysates,(
R2945 T3645 T3650 arg1Of lysates,were
R2946 T3645 T3651 arg2Of lysates,electrophoresed
R2947 T3645 T3656 arg2Of lysates,transferred
R2948 T3648 T3646 arg2Of µg,(
R2949 T3648 T3647 arg1Of µg,70–100
R2950 T3649 T3646 arg3Of ),(
R2951 T3651 T3652 arg1Of electrophoresed,on
R2952 T3651 T3655 arg1Of electrophoresed,and
R2953 T3654 T3652 arg2Of gels,on
R2954 T3654 T3653 arg1Of gels,polyacrylamide
R2955 T3655 T3650 arg2Of and,were
R2956 T3656 T3655 arg2Of transferred,and
R2957 T3656 T3657 arg1Of transferred,to
R2958 T3659 T3657 arg2Of PVDF-membrane,to
R2959 T3659 T3658 arg1Of PVDF-membrane,a
R2960 T3659 T3660 arg1Of PVDF-membrane,(
R2961 T3662 T3660 arg2Of P,(
R2962 T3662 T3661 arg1Of P,Immobilon
R2963 T3662 T3663 arg1Of P,","
R2964 T3662 T3666 arg1Of P,;
R2965 T3665 T3663 arg2Of µm,","
R2966 T3665 T3664 arg1Of µm,0.45
R2967 T3667 T3666 arg2Of Millipore,;
R2968 T3667 T3668 arg1Of Millipore,","
R2969 T3669 T3668 arg2Of Eschborn,","
R2970 T3669 T3670 arg1Of Eschborn,","
R2971 T3671 T3670 arg2Of Germany,","
R2972 T3672 T3660 arg3Of ),(
R2973 T3673 T3674 arg1Of Membranes,were
R2974 T3673 T3675 arg2Of Membranes,blocked
R2975 T3673 T3715 arg2Of Membranes,incubated
R2976 T3675 T3676 arg1Of blocked,with
R2977 T3675 T3713 arg1Of blocked,and
R2978 T3678 T3676 arg2Of %,with
R2979 T3678 T3677 arg1Of %,2.5
R2980 T3678 T3679 arg1Of %,blocking
R2981 T3678 T3690 arg1Of %,(
R2982 T3680 T3679 arg2Of reagent,blocking
R2983 T3680 T3681 arg1Of reagent,(
R2984 T3680 T3687 arg1Of reagent,in
R2985 T3683 T3681 arg2Of Mannheim,(
R2986 T3683 T3682 arg1Of Mannheim,Boehringer
R2987 T3683 T3684 arg1Of Mannheim,","
R2988 T3685 T3684 arg2Of Germany,","
R2989 T3686 T3681 arg3Of ),(
R2990 T3689 T3687 arg2Of buffer,in
R2991 T3689 T3688 arg1Of buffer,TBST
R2992 T3693 T3691 arg1Of Tris–HCL,4.44
R2993 T3693 T3692 arg1Of Tris–HCL,g/l
R2994 T3693 T3694 arg1Of Tris–HCL,","
R2995 T3694 T3698 arg1Of ",",","
R2996 T3697 T3694 arg2Of TrisOH,","
R2997 T3697 T3695 arg1Of TrisOH,2.65
R2998 T3697 T3696 arg1Of TrisOH,g/l
R2999 T3698 T3702 arg1Of ",",","
R3000 T3701 T3698 arg2Of NaCl,","
R3001 T3701 T3699 arg1Of NaCl,8.07
R3002 T3701 T3700 arg1Of NaCl,g/l
R3003 T3702 T3706 arg1Of ",",and
R3004 T3703 T3704 arg1Of 0.2,g/l
R3005 T3705 T3702 arg2Of KCl,","
R3006 T3705 T3703 arg1Of KCl,0.2
R3007 T3706 T3708 arg1Of and,µl/l
R3008 T3707 T3706 arg2Of 500,and
R3009 T3708 T3690 arg2Of µl/l,(
R3010 T3708 T3710 arg1Of µl/l,in
R3011 T3709 T3708 arg2Of Tween-20,µl/l
R3012 T3711 T3710 arg2Of H2O,in
R3013 T3712 T3690 arg3Of ),(
R3014 T3713 T3674 arg2Of and,were
R3015 T3713 T3739 arg1Of and,respectively
R3016 T3715 T3713 arg2Of incubated,and
R3017 T3715 T3714 arg1Of incubated,subsequently
R3018 T3715 T3716 arg1Of incubated,with
R3019 T3715 T3738 arg1Of incubated,","
R3020 T3718 T3716 arg2Of antibody,with
R3021 T3718 T3717 arg1Of antibody,primary
R3022 T3718 T3719 arg1Of antibody,as
R3023 T3723 T3721 arg1Of peroxidase-conjugated,and
R3024 T3723 T3722 arg1Of peroxidase-conjugated,horseradish
R3025 T3725 T3720 arg2Of antibody,indicated
R3026 T3725 T3723 arg1Of antibody,peroxidase-conjugated
R3027 T3725 T3724 arg1Of antibody,secondary
R3028 T3725 T3726 arg1Of antibody,","
R3029 T3726 T3719 arg2Of ",",as
R3030 T3726 T3731 arg1Of ",",(
R3031 T3727 T3728 arg1Of anti-mouse,or
R3032 T3729 T3728 arg2Of anti-goat,or
R3033 T3730 T3726 arg2Of IgG,","
R3034 T3730 T3727 arg1Of IgG,anti-mouse
R3035 T3730 T3729 arg1Of IgG,anti-goat
R3036 T3732 T3733 arg1Of DAKO,","
R3037 T3733 T3735 arg1Of ",",","
R3038 T3734 T3733 arg2Of Hamburg,","
R3039 T3735 T3731 arg2Of ",",(
R3040 T3736 T3735 arg2Of Germany,","
R3041 T3737 T3731 arg3Of ),(
R3042 T3741 T3740 arg1Of membranes,The
R3043 T3741 T3742 arg1Of membranes,were
R3044 T3741 T3744 arg2Of membranes,developed
R3045 T3744 T3742 arg2Of developed,were
R3046 T3744 T3743 arg1Of developed,then
R3047 T3744 T3745 arg1Of developed,with
R3048 T3749 T3745 arg2Of kit,with
R3049 T3749 T3746 arg1Of kit,an
R3050 T3749 T3747 arg1Of kit,ECL
R3051 T3749 T3748 arg1Of kit,detection
R3052 T3749 T3750 arg1Of kit,(
R3053 T3753 T3750 arg2Of Biotech,(
R3054 T3753 T3751 arg1Of Biotech,Amersham
R3055 T3753 T3752 arg1Of Biotech,Pharmacia
R3056 T3753 T3754 arg1Of Biotech,","
R3057 T3755 T3754 arg2Of Freiburg,","
R3058 T3755 T3756 arg1Of Freiburg,","
R3059 T3757 T3756 arg2Of Germany,","
R3060 T3758 T3750 arg3Of ),(
R3061 T3761 T3759 arg1Of antibodies,The
R3062 T3761 T3760 arg1Of antibodies,primary
R3063 T3761 T3762 arg1Of antibodies,were
R3064 T3764 T3763 arg1Of anti-IRF-4/ICSAT,goat
R3065 T3764 T3765 arg1Of anti-IRF-4/ICSAT,(
R3066 T3764 T3768 arg1Of anti-IRF-4/ICSAT,(
R3067 T3764 T3778 arg1Of anti-IRF-4/ICSAT,and
R3068 T3766 T3765 arg2Of M-17,(
R3069 T3767 T3765 arg3Of ),(
R3070 T3771 T3768 arg2Of Biotechnology,(
R3071 T3771 T3769 arg1Of Biotechnology,Santa
R3072 T3771 T3770 arg1Of Biotechnology,Cruz
R3073 T3771 T3772 arg1Of Biotechnology,","
R3074 T3774 T3772 arg2Of Cruz,","
R3075 T3774 T3773 arg1Of Cruz,Santa
R3076 T3774 T3775 arg1Of Cruz,","
R3077 T3776 T3775 arg2Of CA,","
R3078 T3777 T3768 arg3Of ),(
R3079 T3778 T3762 arg2Of and,were
R3080 T3778 T3784 arg1Of and,(
R3081 T3782 T3778 arg2Of AC-74,and
R3082 T3782 T3779 arg1Of AC-74,mouse
R3083 T3782 T3780 arg1Of AC-74,anti-β-actin
R3084 T3782 T3781 arg2Of AC-74,(
R3085 T3783 T3781 arg3Of ),(
R3086 T3785 T3784 arg2Of Sigma,(
R3087 T3786 T3784 arg3Of ),(

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T3802 0-10 NN denotes Expression
T3803 11-19 NN denotes analysis
T3804 20-22 TO denotes To
T3805 23-30 VB denotes analyze
T3806 31-34 DT denotes the
T3807 35-40 NN denotes IRF-4
T3808 41-56 JJ denotes transcriptional
T3809 57-62 NN denotes level
T3810 62-63 , denotes ,
T3811 64-67 NNP denotes RNA
T3812 68-71 VBD denotes was
T3813 72-81 VBN denotes extracted
T3814 82-86 IN denotes from
T3815 87-92 NNS denotes cells
T3816 93-98 VBG denotes using
T3817 99-102 DT denotes the
T3818 103-113 JJ denotes commercial
T3819 114-124 NN denotes RNAzol-kit
T3820 125-126 -LRB- denotes (
T3821 126-132 NNP denotes Paesel
T3822 132-133 , denotes ,
T3823 134-143 NNP denotes Frankfurt
T3824 143-144 , denotes ,
T3825 145-152 NNP denotes Germany
T3826 152-153 -RRB- denotes )
T3827 153-154 . denotes .
T3828 155-157 DT denotes An
T3829 158-165 NN denotes aliquot
T3830 166-168 IN denotes of
T3831 169-170 CD denotes 1
T3832 171-173 NN denotes μg
T3833 174-179 JJ denotes total
T3834 180-183 NNP denotes RNA
T3835 184-187 VBD denotes was
T3836 188-192 VBN denotes used
T3837 193-196 IN denotes for
T3838 197-201 NNP denotes cDNA
T3839 202-211 NN denotes synthesis
T3840 212-214 IN denotes as
T3841 215-224 VBN denotes described
T3842 225-235 RB denotes previously
T3843 236-237 -LRB- denotes (
T3844 237-239 CD denotes 27
T3845 239-240 -RRB- denotes )
T3846 240-241 . denotes .
T3847 242-245 NNP denotes RNA
T3848 246-256 NN denotes expression
T3849 257-265 NN denotes analysis
T3850 266-269 IN denotes for
T3851 270-275 NNP denotes IRF-4
T3852 276-279 CC denotes and
T3853 280-283 DT denotes the
T3854 284-293 NN denotes reference
T3855 294-298 NN denotes gene
T3856 299-306 NN denotes β-actin
T3857 307-310 VBD denotes was
T3858 311-318 VBN denotes carried
T3859 319-322 RP denotes out
T3860 323-325 IN denotes by
T3861 326-343 JJ denotes semi-quantitative
T3862 344-347 NNP denotes PCR
T3863 348-350 IN denotes as
T3864 351-360 VBN denotes described
T3865 361-371 RB denotes previously
T3866 372-373 -LRB- denotes (
T3867 373-377 CD denotes 3,27
T3868 377-378 -RRB- denotes )
T3869 378-379 . denotes .
T3870 380-383 NNP denotes PCR
T3871 384-392 NNS denotes products
T3872 393-397 VBD denotes were
T3873 398-406 VBN denotes verified
T3874 407-409 IN denotes by
T3875 410-419 JJ denotes automated
T3876 420-430 NN denotes sequencing
T3877 430-431 . denotes .
T3878 432-435 NNP denotes PCR
T3879 436-443 NNS denotes primers
T3880 444-447 CC denotes and
T3881 448-458 NNS denotes conditions
T3882 459-462 IN denotes for
T3883 463-473 NN denotes expression
T3884 474-482 NN denotes analysis
T3885 483-485 IN denotes of
T3886 486-490 NNP denotes DNMT
T3887 491-493 CC denotes or
T3888 494-497 NNP denotes MBP
T3889 498-499 -LRB- denotes (
T3890 499-504 NNP denotes DNMT1
T3891 505-511 NNP denotes DNMT3A
T3892 511-512 , denotes ,
T3893 513-519 NNP denotes DNMT3B
T3894 519-520 , denotes ,
T3895 521-525 NNP denotes MeCP
T3896 525-526 , denotes ,
T3897 527-531 NNP denotes MBD1
T3898 531-532 , denotes ,
T3899 533-537 NNP denotes MBD2
T3900 538-541 CC denotes and
T3901 542-546 NNP denotes MBD4
T3902 546-547 -RRB- denotes )
T3903 548-552 VBD denotes were
T3904 553-562 VBN denotes published
T3905 563-572 RB denotes elsewhere
T3906 573-574 -LRB- denotes (
T3907 574-576 CD denotes 28
T3908 576-577 -RRB- denotes )
T3909 577-578 . denotes .
T3910 579-582 IN denotes For
T3911 583-591 NN denotes analysis
T3912 592-594 IN denotes of
T3913 595-600 NN denotes IRF-4
T3914 601-608 NN denotes protein
T3915 609-619 NN denotes expression
T3916 619-620 , denotes ,
T3917 621-622 DT denotes a
T3918 623-631 JJ denotes standard
T3919 632-646 NN denotes immunoblotting
T3920 647-652 NN denotes assay
T3921 653-656 VBD denotes was
T3922 657-666 VBN denotes performed
T3923 667-669 IN denotes as
T3924 670-679 VBN denotes described
T3925 680-690 RB denotes previously
T3926 691-692 -LRB- denotes (
T3927 692-694 CD denotes 29
T3928 694-695 -RRB- denotes )
T3929 695-696 . denotes .
T3930 697-704 RB denotes Briefly
T3931 704-705 , denotes ,
T3932 706-713 NN denotes protein
T3933 714-721 NNS denotes lysates
T3934 722-726 VBD denotes were
T3935 727-736 VBN denotes generated
T3936 737-739 IN denotes by
T3937 740-750 VBG denotes incubating
T3938 751-752 CD denotes 1
T3939 753-754 CD denotes ×
T3940 755-758 CD denotes 106
T3941 759-764 NNS denotes cells
T3942 765-767 IN denotes in
T3943 768-771 CD denotes 100
T3944 772-774 NN denotes µl
T3945 775-779 NNP denotes RIPA
T3946 780-786 NN denotes buffer
T3947 787-788 -LRB- denotes (
T3948 788-789 CD denotes 1
T3949 789-790 NN denotes %
T3950 791-796 NN denotes NP-40
T3951 796-797 , denotes ,
T3952 798-801 CD denotes 0.5
T3953 801-802 NN denotes %
T3954 803-822 NN denotes sodiumdesoxycholate
T3955 822-823 , denotes ,
T3956 824-827 CD denotes 0.1
T3957 827-828 NN denotes %
T3958 829-832 NNP denotes SDS
T3959 832-833 , denotes ,
T3960 834-837 CD denotes 100
T3961 838-840 NN denotes µg
T3962 840-841 NN denotes /
T3963 841-843 NN denotes ml
T3964 844-864 NN denotes phenylmethylsulfonyl
T3965 865-873 NN denotes fluoride
T3966 873-874 , denotes ,
T3967 875-877 CD denotes 10
T3968 878-880 NN denotes µl
T3969 880-881 NN denotes /
T3970 881-883 NN denotes ml
T3971 884-907 NN denotes protease-inhibitory-mix
T3972 907-908 , denotes ,
T3973 909-910 CD denotes 1
T3974 911-915 NN denotes µmol
T3975 915-916 CD denotes /
T3976 916-918 NN denotes ml
T3977 919-938 NN denotes sodiumorthovanadate
T3978 939-941 IN denotes in
T3979 942-960 JJ denotes phosphate-buffered
T3980 961-967 NN denotes saline
T3981 967-968 -RRB- denotes )
T3982 969-972 IN denotes for
T3983 973-975 CD denotes 30
T3984 976-979 NN denotes min
T3985 980-982 IN denotes on
T3986 983-986 NN denotes ice
T3987 986-987 . denotes .
T3988 988-993 IN denotes After
T3989 994-1008 NN denotes centrifugation
T3990 1008-1009 , denotes ,
T3991 1010-1017 NN denotes protein
T3992 1018-1031 NN denotes concentration
T3993 1032-1034 IN denotes of
T3994 1035-1038 DT denotes the
T3995 1039-1050 NN denotes supernatant
T3996 1051-1054 VBD denotes was
T3997 1055-1065 VBN denotes determined
T3998 1066-1068 IN denotes by
T3999 1069-1079 JJ denotes BCA-method
T4000 1080-1081 -LRB- denotes (
T4001 1081-1087 NNP denotes Pierce
T4002 1087-1088 , denotes ,
T4003 1089-1097 NNP denotes Rockford
T4004 1097-1098 , denotes ,
T4005 1099-1101 NNP denotes IL
T4006 1101-1102 -RRB- denotes )
T4007 1103-1105 IN denotes as
T4008 1106-1117 VBN denotes recommended
T4009 1117-1118 . denotes .
T4010 1119-1126 NNP denotes Protein
T4011 1127-1134 VBZ denotes lysates
T4012 1135-1136 -LRB- denotes (
T4013 1136-1138 CD denotes 70
T4014 1139-1142 CD denotes 100
T4015 1143-1145 NN denotes µg
T4016 1145-1146 -RRB- denotes )
T4017 1147-1151 VBD denotes were
T4018 1152-1167 VBN denotes electrophoresed
T4019 1168-1170 IN denotes on
T4020 1171-1185 NN denotes polyacrylamide
T4021 1186-1190 NNS denotes gels
T4022 1191-1194 CC denotes and
T4023 1195-1206 VBN denotes transferred
T4024 1207-1209 TO denotes to
T4025 1210-1211 DT denotes a
T4026 1212-1225 NN denotes PVDF-membrane
T4027 1226-1227 -LRB- denotes (
T4028 1227-1236 NNP denotes Immobilon
T4029 1237-1238 NNP denotes P
T4030 1238-1239 , denotes ,
T4031 1240-1244 CD denotes 0.45
T4032 1245-1247 NN denotes µm
T4033 1247-1248 : denotes ;
T4034 1249-1258 NNP denotes Millipore
T4035 1258-1259 , denotes ,
T4036 1260-1268 NNP denotes Eschborn
T4037 1268-1269 , denotes ,
T4038 1270-1277 NNP denotes Germany
T4039 1277-1278 -RRB- denotes )
T4040 1278-1279 . denotes .
T4041 1280-1289 NNS denotes Membranes
T4042 1290-1294 VBD denotes were
T4043 1295-1302 VBN denotes blocked
T4044 1303-1307 IN denotes with
T4045 1308-1311 CD denotes 2.5
T4046 1311-1312 NN denotes %
T4047 1313-1321 VBG denotes blocking
T4048 1322-1329 NN denotes reagent
T4049 1330-1331 -LRB- denotes (
T4050 1331-1341 NNP denotes Boehringer
T4051 1342-1350 NNP denotes Mannheim
T4052 1350-1351 , denotes ,
T4053 1352-1359 NNP denotes Germany
T4054 1359-1360 -RRB- denotes )
T4055 1361-1363 IN denotes in
T4056 1364-1368 NNP denotes TBST
T4057 1369-1375 NN denotes buffer
T4058 1376-1377 -LRB- denotes (
T4059 1377-1381 CD denotes 4.44
T4060 1382-1385 NN denotes g/l
T4061 1386-1390 NNP denotes Tris
T4062 1391-1394 NNP denotes HCL
T4063 1394-1395 , denotes ,
T4064 1396-1400 CD denotes 2.65
T4065 1401-1404 NN denotes g/l
T4066 1405-1411 NNP denotes TrisOH
T4067 1411-1412 , denotes ,
T4068 1413-1417 CD denotes 8.07
T4069 1418-1421 NN denotes g/l
T4070 1422-1426 NNP denotes NaCl
T4071 1426-1427 , denotes ,
T4072 1428-1431 CD denotes 0.2
T4073 1432-1435 NN denotes g/l
T4074 1436-1439 NNP denotes KCl
T4075 1440-1443 CC denotes and
T4076 1444-1447 CD denotes 500
T4077 1448-1450 NN denotes µl
T4078 1450-1451 NN denotes /
T4079 1451-1452 NN denotes l
T4080 1453-1461 NN denotes Tween-20
T4081 1462-1464 IN denotes in
T4082 1465-1468 CD denotes H2O
T4083 1468-1469 -RRB- denotes )
T4084 1470-1473 CC denotes and
T4085 1474-1486 RB denotes subsequently
T4086 1487-1496 VBN denotes incubated
T4087 1497-1501 IN denotes with
T4088 1502-1509 JJ denotes primary
T4089 1510-1518 NN denotes antibody
T4090 1519-1521 IN denotes as
T4091 1522-1531 VBN denotes indicated
T4092 1532-1535 CC denotes and
T4093 1536-1547 JJ denotes horseradish
T4094 1548-1569 JJ denotes peroxidase-conjugated
T4095 1570-1579 JJ denotes secondary
T4096 1580-1588 NN denotes antibody
T4097 1588-1589 , denotes ,
T4098 1590-1600 JJ denotes anti-mouse
T4099 1601-1603 CC denotes or
T4100 1604-1613 JJ denotes anti-goat
T4101 1614-1617 NNP denotes IgG
T4102 1618-1619 -LRB- denotes (
T4103 1619-1623 NNP denotes DAKO
T4104 1623-1624 , denotes ,
T4105 1625-1632 NNP denotes Hamburg
T4106 1632-1633 , denotes ,
T4107 1634-1641 NNP denotes Germany
T4108 1641-1642 -RRB- denotes )
T4109 1642-1643 , denotes ,
T4110 1644-1656 RB denotes respectively
T4111 1656-1657 . denotes .
T4112 1658-1661 DT denotes The
T4113 1662-1671 NNS denotes membranes
T4114 1672-1676 VBD denotes were
T4115 1677-1681 RB denotes then
T4116 1682-1691 VBN denotes developed
T4117 1692-1696 IN denotes with
T4118 1697-1699 DT denotes an
T4119 1700-1703 NNP denotes ECL
T4120 1704-1713 NN denotes detection
T4121 1714-1717 NN denotes kit
T4122 1718-1719 -LRB- denotes (
T4123 1719-1727 NNP denotes Amersham
T4124 1728-1737 NNP denotes Pharmacia
T4125 1738-1745 NNP denotes Biotech
T4126 1745-1746 , denotes ,
T4127 1747-1755 NNP denotes Freiburg
T4128 1755-1756 , denotes ,
T4129 1757-1764 NNP denotes Germany
T4130 1764-1765 -RRB- denotes )
T4131 1765-1766 . denotes .
T4132 1767-1770 DT denotes The
T4133 1771-1778 JJ denotes primary
T4134 1779-1789 NNS denotes antibodies
T4135 1790-1794 VBD denotes were
T4136 1795-1799 JJ denotes goat
T4137 1800-1810 JJ denotes anti-IRF-4
T4138 1810-1811 NN denotes /
T4139 1811-1816 NNP denotes ICSAT
T4140 1817-1818 -LRB- denotes (
T4141 1818-1822 NNP denotes M-17
T4142 1822-1823 -RRB- denotes )
T4143 1824-1825 -LRB- denotes (
T4144 1825-1830 NNP denotes Santa
T4145 1831-1835 NNP denotes Cruz
T4146 1836-1849 NNP denotes Biotechnology
T4147 1849-1850 , denotes ,
T4148 1851-1856 NNP denotes Santa
T4149 1857-1861 NNP denotes Cruz
T4150 1861-1862 , denotes ,
T4151 1863-1865 NNP denotes CA
T4152 1865-1866 -RRB- denotes )
T4153 1867-1870 CC denotes and
T4154 1871-1876 NN denotes mouse
T4155 1877-1889 NN denotes anti-β-actin
T4156 1890-1891 -LRB- denotes (
T4157 1891-1896 NN denotes AC-74
T4158 1896-1897 -RRB- denotes )
T4159 1898-1899 -LRB- denotes (
T4160 1899-1904 NNP denotes Sigma
T4161 1904-1905 -RRB- denotes )
T4162 1905-1906 . denotes .
R3088 T3802 T3803 compound Expression,analysis
R3089 T3803 T3813 nsubjpass analysis,extracted
R3090 T3804 T3805 aux To,analyze
R3091 T3805 T3813 advcl analyze,extracted
R3092 T3806 T3809 det the,level
R3093 T3807 T3809 nmod IRF-4,level
R3094 T3808 T3809 amod transcriptional,level
R3095 T3809 T3805 dobj level,analyze
R3096 T3810 T3813 punct ",",extracted
R3097 T3811 T3813 nsubjpass RNA,extracted
R3098 T3812 T3813 auxpass was,extracted
R3099 T3813 T3813 ROOT extracted,extracted
R3100 T3814 T3813 prep from,extracted
R3101 T3815 T3814 pobj cells,from
R3102 T3816 T3813 advcl using,extracted
R3103 T3817 T3819 det the,RNAzol-kit
R3104 T3818 T3819 amod commercial,RNAzol-kit
R3105 T3819 T3816 dobj RNAzol-kit,using
R3106 T3820 T3819 punct (,RNAzol-kit
R3107 T3821 T3819 appos Paesel,RNAzol-kit
R3108 T3822 T3821 punct ",",Paesel
R3109 T3823 T3821 npadvmod Frankfurt,Paesel
R3110 T3824 T3823 punct ",",Frankfurt
R3111 T3825 T3823 conj Germany,Frankfurt
R3112 T3826 T3821 punct ),Paesel
R3113 T3827 T3813 punct .,extracted
R3114 T3828 T3829 det An,aliquot
R3115 T3829 T3836 nsubjpass aliquot,used
R3116 T3830 T3829 prep of,aliquot
R3117 T3831 T3832 nummod 1,μg
R3118 T3832 T3830 pobj μg,of
R3119 T3833 T3834 amod total,RNA
R3120 T3834 T3829 appos RNA,aliquot
R3121 T3835 T3836 auxpass was,used
R3122 T3836 T3836 ROOT used,used
R3123 T3837 T3836 prep for,used
R3124 T3838 T3839 compound cDNA,synthesis
R3125 T3839 T3837 pobj synthesis,for
R3126 T3840 T3841 mark as,described
R3127 T3841 T3836 advcl described,used
R3128 T3842 T3841 advmod previously,described
R3129 T3843 T3844 punct (,27
R3130 T3844 T3841 npadvmod 27,described
R3131 T3845 T3836 punct ),used
R3132 T3846 T3836 punct .,used
R3133 T3847 T3849 compound RNA,analysis
R3134 T3848 T3849 compound expression,analysis
R3135 T3849 T3858 nsubjpass analysis,carried
R3136 T3850 T3849 prep for,analysis
R3137 T3851 T3850 pobj IRF-4,for
R3138 T3852 T3851 cc and,IRF-4
R3139 T3853 T3855 det the,gene
R3140 T3854 T3855 compound reference,gene
R3141 T3855 T3856 compound gene,β-actin
R3142 T3856 T3858 nsubjpass β-actin,carried
R3143 T3857 T3858 auxpass was,carried
R3144 T3858 T3858 ROOT carried,carried
R3145 T3859 T3858 prt out,carried
R3146 T3860 T3858 agent by,carried
R3147 T3861 T3862 compound semi-quantitative,PCR
R3148 T3862 T3860 pobj PCR,by
R3149 T3863 T3864 mark as,described
R3150 T3864 T3858 advcl described,carried
R3151 T3865 T3864 advmod previously,described
R3152 T3866 T3864 punct (,described
R3153 T3867 T3864 npadvmod "3,27",described
R3154 T3868 T3864 punct ),described
R3155 T3869 T3858 punct .,carried
R3156 T3870 T3871 compound PCR,products
R3157 T3871 T3873 nsubjpass products,verified
R3158 T3872 T3873 auxpass were,verified
R3159 T3873 T3873 ROOT verified,verified
R3160 T3874 T3873 agent by,verified
R3161 T3875 T3876 amod automated,sequencing
R3162 T3876 T3874 pobj sequencing,by
R3163 T3877 T3873 punct .,verified
R3164 T3878 T3879 compound PCR,primers
R3165 T3879 T3904 nsubjpass primers,published
R3166 T3880 T3879 cc and,primers
R3167 T3881 T3879 conj conditions,primers
R3168 T3882 T3879 prep for,primers
R3169 T3883 T3884 compound expression,analysis
R3170 T3884 T3882 pobj analysis,for
R3171 T3885 T3884 prep of,analysis
R3172 T3886 T3885 pobj DNMT,of
R3173 T3887 T3886 cc or,DNMT
R3174 T3888 T3886 conj MBP,DNMT
R3175 T3889 T3888 punct (,MBP
R3176 T3890 T3891 compound DNMT1,DNMT3A
R3177 T3891 T3888 appos DNMT3A,MBP
R3178 T3892 T3891 punct ",",DNMT3A
R3179 T3893 T3891 conj DNMT3B,DNMT3A
R3180 T3894 T3893 punct ",",DNMT3B
R3181 T3895 T3893 conj MeCP,DNMT3B
R3182 T3896 T3895 punct ",",MeCP
R3183 T3897 T3895 conj MBD1,MeCP
R3184 T3898 T3897 punct ",",MBD1
R3185 T3899 T3897 conj MBD2,MBD1
R3186 T3900 T3899 cc and,MBD2
R3187 T3901 T3899 conj MBD4,MBD2
R3188 T3902 T3888 punct ),MBP
R3189 T3903 T3904 auxpass were,published
R3190 T3904 T3904 ROOT published,published
R3191 T3905 T3904 advmod elsewhere,published
R3192 T3906 T3904 punct (,published
R3193 T3907 T3904 npadvmod 28,published
R3194 T3908 T3904 punct ),published
R3195 T3909 T3904 punct .,published
R3196 T3910 T3922 prep For,performed
R3197 T3911 T3910 pobj analysis,For
R3198 T3912 T3911 prep of,analysis
R3199 T3913 T3915 compound IRF-4,expression
R3200 T3914 T3915 compound protein,expression
R3201 T3915 T3912 pobj expression,of
R3202 T3916 T3922 punct ",",performed
R3203 T3917 T3920 det a,assay
R3204 T3918 T3920 amod standard,assay
R3205 T3919 T3920 compound immunoblotting,assay
R3206 T3920 T3922 nsubjpass assay,performed
R3207 T3921 T3922 auxpass was,performed
R3208 T3922 T3922 ROOT performed,performed
R3209 T3923 T3924 mark as,described
R3210 T3924 T3922 advcl described,performed
R3211 T3925 T3924 advmod previously,described
R3212 T3926 T3927 punct (,29
R3213 T3927 T3924 npadvmod 29,described
R3214 T3928 T3922 punct ),performed
R3215 T3929 T3922 punct .,performed
R3216 T3930 T3935 advmod Briefly,generated
R3217 T3931 T3935 punct ",",generated
R3218 T3932 T3933 compound protein,lysates
R3219 T3933 T3935 nsubjpass lysates,generated
R3220 T3934 T3935 auxpass were,generated
R3221 T3935 T3935 ROOT generated,generated
R3222 T3936 T3935 prep by,generated
R3223 T3937 T3936 pcomp incubating,by
R3224 T3938 T3941 nummod 1,cells
R3225 T3939 T3941 nummod ×,cells
R3226 T3940 T3941 nummod 106,cells
R3227 T3941 T3937 dobj cells,incubating
R3228 T3942 T3937 prep in,incubating
R3229 T3943 T3944 nummod 100,µl
R3230 T3944 T3946 compound µl,buffer
R3231 T3945 T3946 compound RIPA,buffer
R3232 T3946 T3942 pobj buffer,in
R3233 T3947 T3950 punct (,NP-40
R3234 T3948 T3949 nummod 1,%
R3235 T3949 T3950 compound %,NP-40
R3236 T3950 T3946 appos NP-40,buffer
R3237 T3951 T3950 punct ",",NP-40
R3238 T3952 T3953 nummod 0.5,%
R3239 T3953 T3954 compound %,sodiumdesoxycholate
R3240 T3954 T3950 appos sodiumdesoxycholate,NP-40
R3241 T3955 T3954 punct ",",sodiumdesoxycholate
R3242 T3956 T3957 nummod 0.1,%
R3243 T3957 T3958 compound %,SDS
R3244 T3958 T3950 appos SDS,NP-40
R3245 T3959 T3958 punct ",",SDS
R3246 T3960 T3961 nummod 100,µg
R3247 T3961 T3965 nmod µg,fluoride
R3248 T3962 T3965 nmod /,fluoride
R3249 T3963 T3965 nmod ml,fluoride
R3250 T3964 T3965 compound phenylmethylsulfonyl,fluoride
R3251 T3965 T3958 appos fluoride,SDS
R3252 T3966 T3965 punct ",",fluoride
R3253 T3967 T3968 nummod 10,µl
R3254 T3968 T3971 nmod µl,protease-inhibitory-mix
R3255 T3969 T3971 nmod /,protease-inhibitory-mix
R3256 T3970 T3971 compound ml,protease-inhibitory-mix
R3257 T3971 T3965 conj protease-inhibitory-mix,fluoride
R3258 T3972 T3971 punct ",",protease-inhibitory-mix
R3259 T3973 T3975 quantmod 1,/
R3260 T3974 T3975 quantmod µmol,/
R3261 T3975 T3977 nummod /,sodiumorthovanadate
R3262 T3976 T3977 compound ml,sodiumorthovanadate
R3263 T3977 T3971 appos sodiumorthovanadate,protease-inhibitory-mix
R3264 T3978 T3977 prep in,sodiumorthovanadate
R3265 T3979 T3980 amod phosphate-buffered,saline
R3266 T3980 T3978 pobj saline,in
R3267 T3981 T3971 punct ),protease-inhibitory-mix
R3268 T3982 T3965 prep for,fluoride
R3269 T3983 T3984 nummod 30,min
R3270 T3984 T3982 pobj min,for
R3271 T3985 T3984 prep on,min
R3272 T3986 T3985 pobj ice,on
R3273 T3987 T3935 punct .,generated
R3274 T3988 T3997 prep After,determined
R3275 T3989 T3988 pobj centrifugation,After
R3276 T3990 T3997 punct ",",determined
R3277 T3991 T3992 compound protein,concentration
R3278 T3992 T3997 nsubjpass concentration,determined
R3279 T3993 T3992 prep of,concentration
R3280 T3994 T3995 det the,supernatant
R3281 T3995 T3993 pobj supernatant,of
R3282 T3996 T3997 auxpass was,determined
R3283 T3997 T3997 ROOT determined,determined
R3284 T3998 T3997 agent by,determined
R3285 T3999 T4001 amod BCA-method,Pierce
R3286 T4000 T4001 punct (,Pierce
R3287 T4001 T3998 pobj Pierce,by
R3288 T4002 T4001 punct ",",Pierce
R3289 T4003 T4006 nmod Rockford,)
R3290 T4004 T4003 punct ",",Rockford
R3291 T4005 T4003 conj IL,Rockford
R3292 T4006 T4001 punct ),Pierce
R3293 T4007 T4008 mark as,recommended
R3294 T4008 T4001 advcl recommended,Pierce
R3295 T4009 T3997 punct .,determined
R3296 T4010 T4011 compound Protein,lysates
R3297 T4011 T4018 nsubjpass lysates,electrophoresed
R3298 T4012 T4011 punct (,lysates
R3299 T4013 T4011 appos 70,lysates
R3300 T4014 T4015 nummod 100,µg
R3301 T4015 T4013 appos µg,70
R3302 T4016 T4013 punct ),70
R3303 T4017 T4018 auxpass were,electrophoresed
R3304 T4018 T4018 ROOT electrophoresed,electrophoresed
R3305 T4019 T4018 prep on,electrophoresed
R3306 T4020 T4021 compound polyacrylamide,gels
R3307 T4021 T4019 pobj gels,on
R3308 T4022 T4018 cc and,electrophoresed
R3309 T4023 T4018 conj transferred,electrophoresed
R3310 T4024 T4023 prep to,transferred
R3311 T4025 T4026 det a,PVDF-membrane
R3312 T4026 T4024 pobj PVDF-membrane,to
R3313 T4027 T4029 punct (,P
R3314 T4028 T4029 compound Immobilon,P
R3315 T4029 T4026 appos P,PVDF-membrane
R3316 T4030 T4029 punct ",",P
R3317 T4031 T4032 nummod 0.45,µm
R3318 T4032 T4029 appos µm,P
R3319 T4033 T4029 punct ;,P
R3320 T4034 T4029 conj Millipore,P
R3321 T4035 T4034 punct ",",Millipore
R3322 T4036 T4034 conj Eschborn,Millipore
R3323 T4037 T4036 punct ",",Eschborn
R3324 T4038 T4036 conj Germany,Eschborn
R3325 T4039 T4029 punct ),P
R3326 T4040 T4018 punct .,electrophoresed
R3327 T4041 T4043 nsubjpass Membranes,blocked
R3328 T4042 T4043 auxpass were,blocked
R3329 T4043 T4043 ROOT blocked,blocked
R3330 T4044 T4043 prep with,blocked
R3331 T4045 T4046 nummod 2.5,%
R3332 T4046 T4047 nsubj %,blocking
R3333 T4047 T4044 pcomp blocking,with
R3334 T4048 T4047 dobj reagent,blocking
R3335 T4049 T4051 punct (,Mannheim
R3336 T4050 T4051 compound Boehringer,Mannheim
R3337 T4051 T4086 nsubj Mannheim,incubated
R3338 T4052 T4051 punct ",",Mannheim
R3339 T4053 T4051 npadvmod Germany,Mannheim
R3340 T4054 T4051 punct ),Mannheim
R3341 T4055 T4051 prep in,Mannheim
R3342 T4056 T4057 compound TBST,buffer
R3343 T4057 T4055 pobj buffer,in
R3344 T4058 T4051 punct (,Mannheim
R3345 T4059 T4060 nummod 4.44,g/l
R3346 T4060 T4061 compound g/l,Tris
R3347 T4061 T4051 appos Tris,Mannheim
R3348 T4062 T4051 appos HCL,Mannheim
R3349 T4063 T4062 punct ",",HCL
R3350 T4064 T4065 nummod 2.65,g/l
R3351 T4065 T4066 compound g/l,TrisOH
R3352 T4066 T4051 appos TrisOH,Mannheim
R3353 T4067 T4051 punct ",",Mannheim
R3354 T4068 T4069 nummod 8.07,g/l
R3355 T4069 T4070 compound g/l,NaCl
R3356 T4070 T4051 appos NaCl,Mannheim
R3357 T4071 T4070 punct ",",NaCl
R3358 T4072 T4073 nummod 0.2,g/l
R3359 T4073 T4074 compound g/l,KCl
R3360 T4074 T4070 conj KCl,NaCl
R3361 T4075 T4074 cc and,KCl
R3362 T4076 T4077 nummod 500,µl
R3363 T4077 T4078 compound µl,/
R3364 T4078 T4080 compound /,Tween-20
R3365 T4079 T4080 compound l,Tween-20
R3366 T4080 T4074 conj Tween-20,KCl
R3367 T4081 T4080 prep in,Tween-20
R3368 T4082 T4081 pobj H2O,in
R3369 T4083 T4080 punct ),Tween-20
R3370 T4084 T4080 cc and,Tween-20
R3371 T4085 T4086 advmod subsequently,incubated
R3372 T4086 T4047 advcl incubated,blocking
R3373 T4087 T4086 prep with,incubated
R3374 T4088 T4089 amod primary,antibody
R3375 T4089 T4087 pobj antibody,with
R3376 T4090 T4091 mark as,indicated
R3377 T4091 T4086 advcl indicated,incubated
R3378 T4092 T4091 cc and,indicated
R3379 T4093 T4096 amod horseradish,antibody
R3380 T4094 T4096 amod peroxidase-conjugated,antibody
R3381 T4095 T4096 amod secondary,antibody
R3382 T4096 T4103 nmod antibody,DAKO
R3383 T4097 T4096 punct ",",antibody
R3384 T4098 T4096 conj anti-mouse,antibody
R3385 T4099 T4098 cc or,anti-mouse
R3386 T4100 T4098 conj anti-goat,anti-mouse
R3387 T4101 T4103 nmod IgG,DAKO
R3388 T4102 T4103 punct (,DAKO
R3389 T4103 T4086 conj DAKO,incubated
R3390 T4104 T4103 punct ",",DAKO
R3391 T4105 T4103 conj Hamburg,DAKO
R3392 T4106 T4105 punct ",",Hamburg
R3393 T4107 T4105 conj Germany,Hamburg
R3394 T4108 T4105 punct ),Hamburg
R3395 T4109 T4103 punct ",",DAKO
R3396 T4110 T4103 npadvmod respectively,DAKO
R3397 T4111 T4043 punct .,blocked
R3398 T4112 T4113 det The,membranes
R3399 T4113 T4116 nsubjpass membranes,developed
R3400 T4114 T4116 auxpass were,developed
R3401 T4115 T4116 advmod then,developed
R3402 T4116 T4116 ROOT developed,developed
R3403 T4117 T4116 prep with,developed
R3404 T4118 T4121 det an,kit
R3405 T4119 T4121 compound ECL,kit
R3406 T4120 T4121 compound detection,kit
R3407 T4121 T4117 pobj kit,with
R3408 T4122 T4121 punct (,kit
R3409 T4123 T4125 compound Amersham,Biotech
R3410 T4124 T4125 compound Pharmacia,Biotech
R3411 T4125 T4121 appos Biotech,kit
R3412 T4126 T4125 punct ",",Biotech
R3413 T4127 T4125 conj Freiburg,Biotech
R3414 T4128 T4127 punct ",",Freiburg
R3415 T4129 T4127 appos Germany,Freiburg
R3416 T4130 T4127 punct ),Freiburg
R3417 T4131 T4116 punct .,developed
R3418 T4132 T4134 det The,antibodies
R3419 T4133 T4134 amod primary,antibodies
R3420 T4134 T4135 nsubj antibodies,were
R3421 T4135 T4135 ROOT were,were
R3422 T4136 T4139 amod goat,ICSAT
R3423 T4137 T4139 amod anti-IRF-4,ICSAT
R3424 T4138 T4139 compound /,ICSAT
R3425 T4139 T4135 attr ICSAT,were
R3426 T4140 T4139 punct (,ICSAT
R3427 T4141 T4139 appos M-17,ICSAT
R3428 T4142 T4139 punct ),ICSAT
R3429 T4143 T4146 punct (,Biotechnology
R3430 T4144 T4145 compound Santa,Cruz
R3431 T4145 T4146 compound Cruz,Biotechnology
R3432 T4146 T4139 conj Biotechnology,ICSAT
R3433 T4147 T4146 punct ",",Biotechnology
R3434 T4148 T4149 compound Santa,Cruz
R3435 T4149 T4146 conj Cruz,Biotechnology
R3436 T4150 T4149 punct ",",Cruz
R3437 T4151 T4149 conj CA,Cruz
R3438 T4152 T4146 punct ),Biotechnology
R3439 T4153 T4146 cc and,Biotechnology
R3440 T4154 T4155 compound mouse,anti-β-actin
R3441 T4155 T4139 conj anti-β-actin,ICSAT
R3442 T4156 T4157 punct (,AC-74
R3443 T4157 T4155 appos AC-74,anti-β-actin
R3444 T4158 T4155 punct ),anti-β-actin
R3445 T4159 T4160 punct (,Sigma
R3446 T4160 T4139 appos Sigma,ICSAT
R3447 T4161 T4139 punct ),ICSAT
R3448 T4162 T4135 punct .,were

GO-BP

Id Subject Object Predicate Lexical cue
T3421 41-56 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T3422 202-211 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T3423 1863-1865 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-MF

Id Subject Object Predicate Lexical cue
T4176 1510-1518 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T4177 1580-1588 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T4178 1779-1789 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T4179 1863-1865 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T4295 35-40 Protein denotes IRF-4
T4296 270-275 Protein denotes IRF-4
T4297 284-300 Protein denotes reference gene β
T4298 301-306 Protein denotes actin
T4299 486-490 Protein denotes DNMT
T4300 494-497 Protein denotes MBP
T4301 499-511 Protein denotes DNMT1 DNMT3A
T4302 513-519 Protein denotes DNMT3B
T4303 521-525 Protein denotes MeCP
T4304 527-531 Protein denotes MBD1
T4305 533-537 Protein denotes MBD2
T4306 542-546 Protein denotes MBD4
T4307 463-473 Gene_expression denotes expression
T4308 463-473 Gene_expression denotes expression
T4309 463-473 Gene_expression denotes expression
T4310 463-473 Gene_expression denotes expression
T4311 463-473 Gene_expression denotes expression
T4312 463-473 Gene_expression denotes expression
T4313 463-473 Gene_expression denotes expression
T4314 463-473 Gene_expression denotes expression
T4315 595-608 Protein denotes IRF-4 protein
T4316 609-619 Gene_expression denotes expression
T4317 1536-1558 Protein denotes horseradish peroxidase
T4318 1604-1617 Protein denotes anti-goat IgG
R3506 T4304 T4312 themeOf MBD1,expression
R3507 T4305 T4313 themeOf MBD2,expression
R3508 T4306 T4314 themeOf MBD4,expression
R3509 T4315 T4316 themeOf IRF-4 protein,expression
R3501 T4299 T4307 themeOf DNMT,expression
R3502 T4300 T4308 themeOf MBP,expression
R3503 T4301 T4309 themeOf DNMT1 DNMT3A,expression
R3504 T4302 T4310 themeOf DNMT3B,expression
R3505 T4303 T4311 themeOf MeCP,expression

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Speculation
T3424 41-56 Transcription denotes transcriptional
T3425 463-473 Gene_expression denotes expression true
T3426 609-619 Gene_expression denotes expression true
T3427 242-256 Transcription denotes RNA expression
T3428 242-256 Transcription denotes RNA expression
T3429 463-473 Gene_expression denotes expression true
T3430 463-473 Gene_expression denotes expression true
T3431 463-473 Gene_expression denotes expression true
T3432 463-473 Gene_expression denotes expression true
T3433 463-473 Gene_expression denotes expression true
T3434 463-473 Gene_expression denotes expression true
T3435 35-40 Protein denotes IRF-4
T3436 542-546 Protein denotes MBD4
T3437 595-600 Protein denotes IRF-4
T3438 1805-1810 Protein denotes IRF-4
T3439 1811-1816 Protein denotes ICSAT
T3440 1818-1822 Protein denotes M-17
T3441 1882-1889 Protein denotes β-actin
T3442 270-275 Protein denotes IRF-4
T3443 299-306 Protein denotes β-actin
T3444 505-511 Protein denotes DNMT3A
T3445 513-519 Protein denotes DNMT3B
T3446 521-525 Protein denotes MeCP
T3447 527-531 Protein denotes MBD1
T3448 533-537 Protein denotes MBD2
T3449 499-504 Protein denotes DNMT1
R2739 T3439 T3438 equivalentTo ICSAT,IRF-4
R2740 T3440 T3438 equivalentTo M-17,IRF-4
R2741 T3442 T3427 themeOf IRF-4,RNA expression
R2742 T3443 T3428 themeOf β-actin,RNA expression
R2743 T3444 T3430 themeOf DNMT3A,expression
R2744 T3445 T3431 themeOf DNMT3B,expression
R2745 T3446 T3432 themeOf MeCP,expression
R2746 T3447 T3433 themeOf MBD1,expression
R2747 T3448 T3434 themeOf MBD2,expression
R2748 T3449 T3429 themeOf DNMT1,expression
R2736 T3435 T3424 themeOf IRF-4,transcriptional
R2737 T3436 T3425 themeOf MBD4,expression
R2738 T3437 T3426 themeOf IRF-4,expression

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T3787 35-40 Q15306 denotes IRF-4
T3788 270-275 Q15306 denotes IRF-4
T3789 299-306 P60709 denotes β-actin
T3790 486-490 P26358 denotes DNMT
T3791 499-504 P26358 denotes DNMT1
T3792 505-511 Q9Y6K1 denotes DNMT3A
T3793 513-519 Q9UBC3 denotes DNMT3B
T3794 521-525 P51608 denotes MeCP
T3795 527-531 Q9UIS9 denotes MBD1
T3796 533-537 Q9UBB5 denotes MBD2
T3797 542-546 O95243 denotes MBD4
T3798 595-600 Q15306 denotes IRF-4
T3799 1805-1810 Q15306 denotes IRF-4
T3800 1811-1816 Q99419 denotes ICSAT
T3801 1882-1889 P60709 denotes β-actin

test2

Id Subject Object Predicate Lexical cue Speculation
T3389 35-40 Protein denotes IRF-4
T3390 41-56 Transcription denotes transcriptional
T3391 242-256 Transcription denotes RNA expression
T3392 270-275 Protein denotes IRF-4 true
T3393 299-306 Protein denotes β-actin true
T3394 463-473 Gene_expression denotes expression true
T3395 499-504 Protein denotes DNMT1 true
T3396 505-511 Protein denotes DNMT3A true
T3397 513-519 Protein denotes DNMT3B true
T3398 521-525 Protein denotes MeCP true
T3399 527-531 Protein denotes MBD1 true
T3400 533-537 Protein denotes MBD2
T3401 542-546 Protein denotes MBD4
T3402 595-600 Protein denotes IRF-4
T3403 609-619 Gene_expression denotes expression
T3404 1805-1810 Protein denotes IRF-4
T3405 1811-1816 Protein denotes ICSAT
T3406 1818-1822 Protein denotes M-17
T3407 1882-1889 Protein denotes β-actin
R2723 T3389 T3390 themeOf IRF-4,transcriptional
R2724 T3392 T3391 themeOf IRF-4,RNA expression
R2725 T3393 T3391 themeOf β-actin,RNA expression
R2726 T3395 T3394 themeOf DNMT1,expression
R2727 T3396 T3394 themeOf DNMT3A,expression
R2728 T3397 T3394 themeOf DNMT3B,expression
R2729 T3398 T3394 themeOf MeCP,expression
R2730 T3399 T3394 themeOf MBD1,expression
R2731 T3400 T3394 themeOf MBD2,expression
R2732 T3401 T3394 themeOf MBD4,expression
R2733 T3402 T3403 themeOf IRF-4,expression
R2734 T3405 T3404 equivalentTo ICSAT,IRF-4
R2735 T3406 T3404 equivalentTo M-17,IRF-4