PMC:1310620 / 5792-6422 JSONTXT

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    craft-sa-dev

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RNA samples extracted from NBEC were reverse transcribed using M-MLV reverse transcriptase and oligo dT primers as previously described [9,11]. Standardized RT (StaRT)-PCR was used for transcript abundance measurement in these studies. With StaRT-PCR, an internal standard for each gene within a standardized mixture of internal standards (SMIS) is included in each PCR reaction. After amplification, products were electrophoresed on an Agilent 2100 Bioanalyzer using DNA Chips with DNA 1000 Kit reagents for visualization according to the manufacturer's protocol (Agilent Technologies Deutschland GmbH, Waldbronn, Germany)."}

    2_test

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    craft-ca-core-ex-dev

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    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T1581","span":{"begin":43,"end":62},"obj":"GO:0001171"},{"id":"T1582","span":{"begin":69,"end":74},"obj":"NCBITaxon:11801"},{"id":"T1583","span":{"begin":110,"end":117},"obj":"SO:0000112"},{"id":"T1584","span":{"begin":191,"end":201},"obj":"SO:0000673"},{"id":"T1585","span":{"begin":288,"end":292},"obj":"SO:0000704"},{"id":"T1586","span":{"begin":502,"end":510},"obj":"CHEBI:33893"}],"text":"Total RNA samples extracted from NBEC were reverse transcribed using M-MLV reverse transcriptase and oligo dT primers as previously described [9,11]. Standardized RT (StaRT)-PCR was used for transcript abundance measurement in these studies. With StaRT-PCR, an internal standard for each gene within a standardized mixture of internal standards (SMIS) is included in each PCR reaction. After amplification, products were electrophoresed on an Agilent 2100 Bioanalyzer using DNA Chips with DNA 1000 Kit reagents for visualization according to the manufacturer's protocol (Agilent Technologies Deutschland GmbH, Waldbronn, Germany)."}