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Id Subject Object Predicate Lexical cue
T1735 2-6 NN denotes site
T1737 7-9 IN denotes of
T1738 10-14 NN denotes exon
T1739 15-16 CD denotes 2
T1740 17-18 -LRB- denotes (
T1741 108-111 NN denotes TGC
T1742 18-25 NN denotes forward
T1743 63-65 NN denotes GG
T1744 25-27 : denotes :
T1745 27-30 NN denotes GCC
T1746 31-34 NN denotes GGA
T1747 35-38 NN denotes TCC
T1748 39-42 NN denotes GAT
T1749 43-46 NN denotes CAG
T1750 47-50 NN denotes CAG
T1751 51-54 NN denotes ACC
T1752 55-58 NN denotes GAT
T1753 59-62 NN denotes TCT
T1754 65-66 : denotes ;
T1755 67-74 NN denotes reverse
T1756 74-76 : denotes :
T1757 76-79 NN denotes GCC
T1758 80-83 NN denotes GGT
T1759 84-87 NN denotes ACC
T1760 88-91 NN denotes ACT
T1761 92-95 NN denotes AGG
T1762 96-99 NN denotes AAG
T1763 100-103 NN denotes GCA
T1764 104-107 NN denotes GAA
T1765 111-112 -RRB- denotes )
T1766 112-113 . denotes .
T1767 113-464 sentence denotes This 2-kb intron fragment was cloned into a TA cloning vector (Invitrogen, Carlsbad, California, United States), then excised by Asp718 digestion and ligated to the 6.8-kb Asp718 fragment of moPrP.XhoI containing exon 2, exon 3, the 3′ UTR, and pBluescript to generate an intermediate vector with all three exons and a central intron but no promoter.
T1768 114-118 DT denotes This
T1769 131-139 NN denotes fragment
T1770 119-120 CD denotes 2
T1771 121-123 NN denotes kb
T1772 120-121 HYPH denotes -
T1773 124-130 NN denotes intron
T1774 144-150 VBN denotes cloned
T1775 140-143 VBD denotes was
T1776 151-155 IN denotes into
T1777 156-157 DT denotes a
T1778 169-175 NN denotes vector
T1779 158-160 NN denotes TA
T1780 161-168 NN denotes cloning
T1781 176-177 -LRB- denotes (
T1782 177-187 NNP denotes Invitrogen
T1783 187-189 , denotes ,
T1784 189-197 NNP denotes Carlsbad
T1785 197-199 , denotes ,
T1786 199-209 NNP denotes California
T1787 209-211 , denotes ,
T1788 211-217 NNP denotes United
T1789 218-224 NNP denotes States
T1790 224-225 -RRB- denotes )
T1791 225-227 , denotes ,
T1792 227-231 RB denotes then
T1793 232-239 VBN denotes excised
T1794 240-242 IN denotes by
T1795 243-249 NN denotes Asp718
T1796 250-259 NN denotes digestion
T1797 260-263 CC denotes and
T1798 264-271 VBN denotes ligated
T1799 272-274 IN denotes to
T1800 275-278 DT denotes the
T1801 293-301 NN denotes fragment
T1802 279-282 CD denotes 6.8
T1803 283-285 NN denotes kb
T1804 282-283 HYPH denotes -
T1805 286-292 NN denotes Asp718
T1806 302-304 IN denotes of
T1807 305-315 NN denotes moPrP.XhoI
T1808 316-326 VBG denotes containing
T1809 327-331 NN denotes exon
T1810 332-333 CD denotes 2
T1811 333-335 , denotes ,
T1812 335-339 NN denotes exon
T1813 340-341 CD denotes 3
T1814 341-343 , denotes ,
T1815 343-346 DT denotes the
T1816 350-353 NN denotes UTR
T1817 347-348 CD denotes 3
T1818 348-349 SYM denotes ′
T1819 353-355 , denotes ,
T1820 355-358 CC denotes and
T1821 359-370 NN denotes pBluescript
T1822 371-373 TO denotes to
T1823 374-382 VB denotes generate
T1824 383-385 DT denotes an
T1825 399-405 NN denotes vector
T1826 386-398 JJ denotes intermediate
T1827 406-410 IN denotes with
T1828 411-414 DT denotes all
T1829 421-426 NNS denotes exons
T1830 415-420 CD denotes three
T1831 427-430 CC denotes and
T1832 431-432 DT denotes a
T1833 441-447 NN denotes intron
T1834 433-440 JJ denotes central
T1835 448-451 CC denotes but
T1836 452-454 DT denotes no
T1837 455-463 NN denotes promoter
T1838 463-464 . denotes .
T1839 464-619 sentence denotes This vector was then opened at the BamHI site introduced by the intron cloning primer, and ligated to the 0.5-kb BamHI-cut tetracycline promoter fragment.
T1840 465-469 DT denotes This
T1841 470-476 NN denotes vector
T1842 486-492 VBN denotes opened
T1843 477-480 VBD denotes was
T1844 481-485 RB denotes then
T1845 493-495 IN denotes at
T1846 496-499 DT denotes the
T1847 506-510 NN denotes site
T1848 500-505 NN denotes BamHI
T1849 511-521 VBN denotes introduced
T1850 522-524 IN denotes by
T1851 525-528 DT denotes the
T1852 544-550 NN denotes primer
T1853 529-535 NN denotes intron
T1854 536-543 NN denotes cloning
T1855 550-552 , denotes ,
T1856 552-555 CC denotes and
T1857 556-563 VBN denotes ligated
T1858 564-566 IN denotes to
T1859 567-570 DT denotes the
T1860 610-618 NN denotes fragment
T1861 571-574 CD denotes 0.5
T1862 575-577 NN denotes kb
T1863 574-575 HYPH denotes -
T1864 578-583 NN denotes BamHI
T1865 584-587 NN denotes cut
T1866 583-584 HYPH denotes -
T1867 588-600 NN denotes tetracycline
T1868 601-609 NN denotes promoter
T1869 618-619 . denotes .
T1870 619-835 sentence denotes This ligation generated a 9.3-kb vector encoding the tetracycline promoter from pTetSplice with two exons, one intron, and the original 3′ UTR of the moPrP.XhoI vector, all carried in the pBluescript cloning vector.
T1871 620-624 DT denotes This
T1872 625-633 NN denotes ligation
T1873 634-643 VBD denotes generated
T1874 644-645 DT denotes a
T1875 653-659 NN denotes vector
T1876 646-649 CD denotes 9.3
T1877 650-652 NN denotes kb
T1878 649-650 HYPH denotes -
T1879 660-668 VBG denotes encoding
T1880 669-672 DT denotes the
T1881 686-694 NN denotes promoter
T1882 673-685 NN denotes tetracycline
T1883 695-699 IN denotes from
T1884 700-710 NN denotes pTetSplice
T1885 711-715 IN denotes with
T1886 716-719 CD denotes two
T1887 720-725 NNS denotes exons
T1888 725-727 , denotes ,
T1889 727-730 CD denotes one
T1890 731-737 NN denotes intron
T1891 737-739 , denotes ,
T1892 739-742 CC denotes and
T1893 743-746 DT denotes the
T1894 759-762 NN denotes UTR
T1895 747-755 JJ denotes original
T1896 756-757 CD denotes 3
T1897 757-758 SYM denotes ′
T1898 763-765 IN denotes of
T1899 766-769 DT denotes the
T1900 781-787 NN denotes vector
T1901 770-780 NN denotes moPrP.XhoI
T1902 787-789 , denotes ,
T1903 789-792 RB denotes all
T1904 793-800 VBN denotes carried
T1905 801-803 IN denotes in
T1906 804-807 DT denotes the
T1907 828-834 NN denotes vector
T1908 808-819 NNP denotes pBluescript
T1909 820-827 NN denotes cloning
T1910 834-835 . denotes .
T1911 835-1285 sentence denotes We incorporated the Swedish (KM570/571NL) and Indiana (V617F) mutations into the mo/huAPP695 cDNA (in BS-KS) by PCR using a four-primer strategy: first, two partially overlapping products were generated in separate reactions using primers that encode the desired mutations (Swedish forward: GGA GAT CTC TGA AGT GAA TCT GGA TGC AGA ATT CCG/Indiana reverse: GGG TGA TGA AAA TCA CGG TTG C; Indiana forward: CAA CCG TGA TTT TCA TCA CCC TGG/M13 reverse).
T1912 836-838 PRP denotes We
T1913 839-851 VBD denotes incorporated
T1914 1029-1038 VBN denotes generated
T1915 852-855 DT denotes the
T1916 898-907 NNS denotes mutations
T1917 856-863 NNP denotes Swedish
T1918 864-865 -LRB- denotes (
T1919 871-876 NN denotes 571NL
T1920 865-870 NN denotes KM570
T1921 870-871 HYPH denotes /
T1922 876-877 -RRB- denotes )
T1923 878-881 CC denotes and
T1924 882-889 NNP denotes Indiana
T1925 890-891 -LRB- denotes (
T1926 891-896 NN denotes V617F
T1927 896-897 -RRB- denotes )
T1928 908-912 IN denotes into
T1929 913-916 DT denotes the
T1930 929-933 NN denotes cDNA
T1931 917-919 NN denotes mo
T1932 920-928 NN denotes huAPP695
T1933 919-920 HYPH denotes /
T1934 934-935 -LRB- denotes (
T1935 935-937 IN denotes in
T1936 938-940 NN denotes BS
T1937 941-943 NN denotes KS
T1938 940-941 HYPH denotes -
T1939 943-944 -RRB- denotes )
T1940 945-947 IN denotes by
T1941 948-951 NN denotes PCR
T1942 952-957 VBG denotes using
T1943 958-959 DT denotes a
T1944 972-980 NN denotes strategy
T1945 960-964 CD denotes four
T1946 965-971 NN denotes primer
T1947 964-965 HYPH denotes -
T1948 980-982 : denotes :
T1949 982-987 RB denotes first
T1950 987-989 , denotes ,
T1951 989-992 CD denotes two
T1952 1015-1023 NNS denotes products
T1953 993-1002 RB denotes partially
T1954 1003-1014 VBG denotes overlapping
T1955 1024-1028 VBD denotes were
T1956 1039-1041 IN denotes in
T1957 1042-1050 JJ denotes separate
T1958 1051-1060 NNS denotes reactions
T1959 1061-1066 VBG denotes using
T1960 1067-1074 NNS denotes primers
T1961 1075-1079 WDT denotes that
T1962 1080-1086 VBP denotes encode
T1963 1087-1090 DT denotes the
T1964 1099-1108 NNS denotes mutations
T1965 1091-1098 VBN denotes desired
T1966 1109-1110 -LRB- denotes (
T1967 1272-1275 NN denotes M13
T1968 1110-1117 NNP denotes Swedish
T1969 1118-1125 NN denotes forward
T1970 1171-1174 NN denotes CCG
T1971 1125-1127 : denotes :
T1972 1127-1130 NN denotes GGA
T1973 1131-1134 NN denotes GAT
T1974 1135-1138 NN denotes CTC
T1975 1139-1142 NN denotes TGA
T1976 1143-1146 NN denotes AGT
T1977 1147-1150 NN denotes GAA
T1978 1151-1154 NN denotes TCT
T1979 1155-1158 NNP denotes GGA
T1980 1159-1162 NNP denotes TGC
T1981 1163-1166 NNP denotes AGA
T1982 1167-1170 NNP denotes ATT
T1983 1220-1221 NN denotes C
T1984 1174-1175 HYPH denotes /
T1985 1175-1182 NNP denotes Indiana
T1986 1183-1190 NN denotes reverse
T1987 1190-1192 : denotes :
T1988 1192-1195 NN denotes GGG
T1989 1196-1199 NN denotes TGA
T1990 1200-1203 NN denotes TGA
T1991 1204-1207 NN denotes AAA
T1992 1208-1211 NN denotes TCA
T1993 1212-1215 NN denotes CGG
T1994 1216-1219 NN denotes TTG
T1995 1221-1222 : denotes ;
T1996 1223-1230 NNP denotes Indiana
T1997 1231-1238 NN denotes forward
T1998 1238-1240 : denotes :
T1999 1240-1243 NN denotes CAA
T2000 1244-1247 NN denotes CCG
T2001 1248-1251 NN denotes TGA
T2002 1252-1255 NN denotes TTT
T2003 1256-1259 NN denotes TCA
T2004 1260-1263 NN denotes TCA
T2005 1264-1267 NN denotes CCC
T2006 1268-1271 NN denotes TGG
T2007 1271-1272 HYPH denotes /
T2008 1276-1283 RB denotes reverse
T2009 1283-1284 -RRB- denotes )
T2010 1284-1285 . denotes .
T2011 1285-1409 sentence denotes The two PCR products were ligated, digested with BglII and ApaI and cloned back into the original mo/huAPP695-BS-KS vector.
T2012 1286-1289 DT denotes The
T2013 1298-1306 NNS denotes products
T2014 1290-1293 CD denotes two
T2015 1294-1297 NN denotes PCR
T2016 1312-1319 VBN denotes ligated
T2017 1307-1311 VBD denotes were
T2018 1319-1321 , denotes ,
T2019 1321-1329 VBN denotes digested
T2020 1330-1334 IN denotes with
T2021 1335-1340 NN denotes BglII
T2022 1341-1344 CC denotes and
T2023 1345-1349 NN denotes ApaI
T2024 1350-1353 CC denotes and
T2025 1354-1360 VBN denotes cloned
T2026 1361-1365 RB denotes back
T2027 1366-1370 IN denotes into
T2028 1371-1374 DT denotes the
T2029 1402-1408 NN denotes vector
T2030 1375-1383 JJ denotes original
T2031 1384-1386 NN denotes mo
T2032 1399-1401 NN denotes KS
T2033 1386-1387 HYPH denotes /
T2034 1387-1395 NN denotes huAPP695
T2035 1395-1396 HYPH denotes -
T2036 1396-1398 NN denotes BS
T2037 1398-1399 HYPH denotes -
T2038 1408-1409 . denotes .
T2039 1409-1536 sentence denotes Finally, the new APP695swe/ind was subcloned into the XhoI site of the moPrP-tetP vector from above to complete the construct.
T2040 1410-1417 RB denotes Finally
T2041 1445-1454 VBN denotes subcloned
T2042 1417-1419 , denotes ,
T2043 1419-1422 DT denotes the
T2044 1437-1440 NN denotes ind
T2045 1423-1426 JJ denotes new
T2046 1427-1436 NN denotes APP695swe
T2047 1436-1437 HYPH denotes /
T2048 1441-1444 VBD denotes was
T2049 1455-1459 IN denotes into
T2050 1460-1463 DT denotes the
T2051 1469-1473 NN denotes site
T2052 1464-1468 NN denotes XhoI
T2053 1474-1476 IN denotes of
T2054 1477-1480 DT denotes the
T2055 1492-1498 NN denotes vector
T2056 1481-1486 NN denotes moPrP
T2057 1487-1491 NN denotes tetP
T2058 1486-1487 HYPH denotes -
T2059 1499-1503 IN denotes from
T2060 1504-1509 RB denotes above
T2061 1510-1512 TO denotes to
T2062 1513-1521 VB denotes complete
T2063 1522-1525 DT denotes the
T2064 1526-1535 NN denotes construct
T2065 1535-1536 . denotes .
T2158 1538-1548 JJ denotes Pronuclear
T2159 1549-1558 NN denotes Injection
T2160 1558-1560 , denotes ,
T2161 1560-1569 NN denotes Screening
T2162 1570-1572 IN denotes of
T2163 1573-1581 NNS denotes Founders
T2164 1581-1583 , denotes ,
T2165 1583-1586 CC denotes and
T2166 1587-1598 NN denotes Maintenance
T2167 1599-1601 IN denotes of
T2168 1602-1605 DT denotes the
T2169 1606-1611 NNS denotes Lines
T2170 1611-1726 sentence denotes The moPrP-tetP-mo/huAPP695swe/ind vector was linearized and the pBluescript domain excised by digestion with NotI.
T2171 1612-1615 DT denotes The
T2172 1646-1652 NN denotes vector
T2173 1616-1621 NN denotes moPrP
T2174 1642-1645 NN denotes ind
T2175 1621-1622 HYPH denotes -
T2176 1622-1626 NN denotes tetP
T2177 1626-1627 HYPH denotes -
T2178 1627-1629 NN denotes mo
T2179 1629-1630 HYPH denotes /
T2180 1630-1641 NN denotes huAPP695swe
T2181 1641-1642 HYPH denotes /
T2182 1657-1667 VBN denotes linearized
T2183 1653-1656 VBD denotes was
T2184 1668-1671 CC denotes and
T2185 1672-1675 DT denotes the
T2186 1688-1694 NN denotes domain
T2187 1676-1687 NNP denotes pBluescript
T2188 1695-1702 VBN denotes excised
T2189 1703-1705 IN denotes by
T2190 1706-1715 NN denotes digestion
T2191 1716-1720 IN denotes with
T2192 1721-1725 NN denotes NotI
T2193 1725-1726 . denotes .
T2194 1726-1832 sentence denotes The purified vector was injected into the pronucleus of fertilized eggs from C57BL/6J × C3HeJ F1 matings.
T2195 1727-1730 DT denotes The
T2196 1740-1746 NN denotes vector
T2197 1731-1739 VBN denotes purified
T2198 1751-1759 VBN denotes injected
T2199 1747-1750 VBD denotes was
T2200 1760-1764 IN denotes into
T2201 1765-1768 DT denotes the
T2202 1769-1779 NN denotes pronucleus
T2203 1780-1782 IN denotes of
T2204 1783-1793 VBN denotes fertilized
T2205 1794-1798 NNS denotes eggs
T2206 1799-1803 IN denotes from
T2207 1804-1809 NN denotes C57BL
T2208 1815-1820 NN denotes C3HeJ
T2209 1809-1810 HYPH denotes /
T2210 1810-1812 NN denotes 6J
T2211 1813-1814 SYM denotes ×
T2212 1824-1831 NNS denotes matings
T2213 1821-1823 NN denotes F1
T2214 1831-1832 . denotes .
T2215 1832-1969 sentence denotes Founder animals were screened for the presence of the transgene by three-way PCR using the S36 and PrP-S/PrP-AS primers described below.
T2216 1833-1840 NN denotes Founder
T2217 1841-1848 NNS denotes animals
T2218 1854-1862 VBN denotes screened
T2219 1849-1853 VBD denotes were
T2220 1863-1866 IN denotes for
T2221 1867-1870 DT denotes the
T2222 1871-1879 NN denotes presence
T2223 1880-1882 IN denotes of
T2224 1883-1886 DT denotes the
T2225 1887-1896 NN denotes transgene
T2226 1897-1899 IN denotes by
T2227 1900-1905 CD denotes three
T2228 1906-1909 NN denotes way
T2229 1905-1906 HYPH denotes -
T2230 1910-1913 NN denotes PCR
T2231 1914-1919 VBG denotes using
T2232 1920-1923 DT denotes the
T2233 1945-1952 NNS denotes primers
T2234 1924-1927 NN denotes S36
T2235 1928-1931 CC denotes and
T2236 1932-1935 NN denotes PrP
T2237 1942-1944 NN denotes AS
T2238 1935-1936 HYPH denotes -
T2239 1936-1937 NN denotes S
T2240 1937-1938 HYPH denotes /
T2241 1938-1941 NN denotes PrP
T2242 1941-1942 HYPH denotes -
T2243 1953-1962 VBN denotes described
T2244 1963-1968 RB denotes below
T2245 1968-1969 . denotes .
T2246 1969-2254 sentence denotes Transgene-positive founders were bred to animals expressing the tetracycline transactivator (tTA) under control of the calcium-calmodulin kinase IIα (CaMKIIα) promoter obtained from Jackson Laboratory [16] (Bar Harbor, Maine, United States; stock # 3010; B6;CBA-TgN[Camk2a-tTA]1Mmay).
T2247 1970-1979 NN denotes Transgene
T2248 1980-1988 JJ denotes positive
T2249 1979-1980 HYPH denotes -
T2250 1989-1997 NNS denotes founders
T2251 2003-2007 VBN denotes bred
T2252 1998-2002 VBD denotes were
T2253 2008-2010 IN denotes to
T2254 2011-2018 NNS denotes animals
T2255 2019-2029 VBG denotes expressing
T2256 2030-2033 DT denotes the
T2257 2047-2061 NN denotes transactivator
T2258 2034-2046 NN denotes tetracycline
T2259 2062-2063 -LRB- denotes (
T2260 2063-2066 NN denotes tTA
T2261 2066-2067 -RRB- denotes )
T2262 2068-2073 IN denotes under
T2263 2074-2081 NN denotes control
T2264 2082-2084 IN denotes of
T2265 2085-2088 DT denotes the
T2266 2129-2137 NN denotes promoter
T2267 2089-2096 NN denotes calcium
T2268 2097-2107 NN denotes calmodulin
T2269 2096-2097 HYPH denotes -
T2270 2115-2118 NN denotes IIα
T2271 2108-2114 NN denotes kinase
T2272 2119-2120 -LRB- denotes (
T2273 2120-2127 NN denotes CaMKIIα
T2274 2127-2128 -RRB- denotes )
T2275 2138-2146 VBN denotes obtained
T2276 2147-2151 IN denotes from
T2277 2152-2159 NNP denotes Jackson
T2278 2160-2170 NNP denotes Laboratory
T2279 2171-2172 -LRB- denotes [
T2280 2172-2174 CD denotes 16
T2281 2174-2175 -RRB- denotes ]
T2282 2176-2177 -LRB- denotes (
T2283 2181-2187 NNP denotes Harbor
T2284 2177-2180 NNP denotes Bar
T2285 2187-2189 , denotes ,
T2286 2189-2194 NNP denotes Maine
T2287 2194-2196 , denotes ,
T2288 2196-2202 NNP denotes United
T2289 2203-2209 NNP denotes States
T2290 2209-2210 : denotes ;
T2291 2211-2216 NN denotes stock
T2292 2247-2252 NN denotes 1Mmay
T2293 2217-2218 SYM denotes #
T2294 2219-2223 CD denotes 3010
T2295 2223-2224 : denotes ;
T2296 2225-2227 NN denotes B6
T2297 2227-2228 : denotes ;
T2298 2228-2231 NN denotes CBA
T2299 2231-2232 HYPH denotes -
T2300 2232-2235 NN denotes TgN
T2301 2235-2236 -LRB- denotes [
T2302 2236-2242 NN denotes Camk2a
T2303 2242-2243 HYPH denotes -
T2304 2243-2246 NN denotes tTA
T2305 2246-2247 -RRB- denotes ]
T2306 2252-2253 -RRB- denotes )
T2307 2253-2254 . denotes .
T2308 2254-2375 sentence denotes The colony was thereafter maintained by crossing single transgenic tTA and APP offspring for each of the four APP lines.
T2309 2255-2258 DT denotes The
T2310 2259-2265 NN denotes colony
T2311 2281-2291 VBN denotes maintained
T2312 2266-2269 VBD denotes was
T2313 2270-2280 RB denotes thereafter
T2314 2292-2294 IN denotes by
T2315 2295-2303 VBG denotes crossing
T2316 2304-2310 JJ denotes single
T2317 2334-2343 NN denotes offspring
T2318 2311-2321 JJ denotes transgenic
T2319 2322-2325 NN denotes tTA
T2320 2326-2329 CC denotes and
T2321 2330-2333 NN denotes APP
T2322 2344-2347 IN denotes for
T2323 2348-2352 DT denotes each
T2324 2353-2355 IN denotes of
T2325 2356-2359 DT denotes the
T2326 2369-2374 NNS denotes lines
T2327 2360-2364 CD denotes four
T2328 2365-2368 NN denotes APP
T2329 2374-2375 . denotes .
T2330 2375-2431 sentence denotes All mice were provided fresh food and water ad libitum.
T2331 2376-2379 DT denotes All
T2332 2380-2384 NNS denotes mice
T2333 2390-2398 VBN denotes provided
T2334 2385-2389 VBD denotes were
T2335 2399-2404 JJ denotes fresh
T2336 2405-2409 NN denotes food
T2337 2410-2413 CC denotes and
T2338 2414-2419 NN denotes water
T2339 2420-2422 FW denotes ad
T2340 2423-2430 FW denotes libitum
T2341 2430-2431 . denotes .
T2342 2431-2588 sentence denotes Animal protocols were approved by both the Johns Hopkins University and the California Institute of Technology Institutional Animal Care and Use Committees.
T2343 2432-2438 NN denotes Animal
T2344 2439-2448 NNS denotes protocols
T2345 2454-2462 VBN denotes approved
T2346 2449-2453 VBD denotes were
T2347 2463-2465 IN denotes by
T2348 2466-2470 DT denotes both
T2349 2489-2499 NNP denotes University
T2350 2471-2474 DT denotes the
T2351 2475-2480 NNP denotes Johns
T2352 2481-2488 NNP denotes Hopkins
T2353 2577-2587 NNPS denotes Committees
T2354 2500-2503 CC denotes and
T2355 2504-2507 DT denotes the
T2356 2519-2528 NNP denotes Institute
T2357 2508-2518 NNP denotes California
T2358 2529-2531 IN denotes of
T2359 2532-2542 NNP denotes Technology
T2360 2543-2556 NNP denotes Institutional
T2361 2557-2563 NNP denotes Animal
T2362 2564-2568 NNP denotes Care
T2363 2569-2572 CC denotes and
T2364 2573-2576 NNP denotes Use
T2365 2587-2588 . denotes .
T2408 2590-2601 NN denotes Doxycycline
T2409 2602-2616 NN denotes Administration
T2410 2616-2752 sentence denotes Doxycycline (dox) was administered through commercially available dox-containing chow (BioServ, Frenchtown, New Jersey, United States).
T2411 2617-2628 NN denotes Doxycycline
T2412 2639-2651 VBN denotes administered
T2413 2629-2630 -LRB- denotes (
T2414 2630-2633 NN denotes dox
T2415 2633-2634 -RRB- denotes )
T2416 2635-2638 VBD denotes was
T2417 2652-2659 IN denotes through
T2418 2660-2672 RB denotes commercially
T2419 2673-2682 JJ denotes available
T2420 2698-2702 NN denotes chow
T2421 2683-2686 NN denotes dox
T2422 2687-2697 VBG denotes containing
T2423 2686-2687 HYPH denotes -
T2424 2703-2704 -LRB- denotes (
T2425 2704-2711 NNP denotes BioServ
T2426 2711-2713 , denotes ,
T2427 2713-2723 NNP denotes Frenchtown
T2428 2723-2725 , denotes ,
T2429 2725-2728 NNP denotes New
T2430 2729-2735 NNP denotes Jersey
T2431 2735-2737 , denotes ,
T2432 2737-2743 NNP denotes United
T2433 2744-2750 NNP denotes States
T2434 2750-2751 -RRB- denotes )
T2435 2751-2752 . denotes .
T2436 2752-2908 sentence denotes The chow contained 200 mg/kg of antibiotic; based on estimated consumption of 5 g per mouse per day, the expected dose to each animal was 1 mg dox per day.
T2437 2753-2756 DT denotes The
T2438 2757-2761 NN denotes chow
T2439 2762-2771 VBD denotes contained
T2440 2887-2890 VBD denotes was
T2441 2772-2775 CD denotes 200
T2442 2776-2778 NN denotes mg
T2443 2778-2779 SYM denotes /
T2444 2779-2781 NN denotes kg
T2445 2782-2784 IN denotes of
T2446 2785-2795 JJ denotes antibiotic
T2447 2795-2796 : denotes ;
T2448 2797-2802 VBN denotes based
T2449 2803-2805 IN denotes on
T2450 2806-2815 VBN denotes estimated
T2451 2816-2827 NN denotes consumption
T2452 2828-2830 IN denotes of
T2453 2831-2832 CD denotes 5
T2454 2833-2834 NN denotes g
T2455 2835-2838 IN denotes per
T2456 2839-2844 NN denotes mouse
T2457 2845-2848 IN denotes per
T2458 2849-2852 NN denotes day
T2459 2852-2854 , denotes ,
T2460 2854-2857 DT denotes the
T2461 2867-2871 NN denotes dose
T2462 2858-2866 VBN denotes expected
T2463 2872-2874 IN denotes to
T2464 2875-2879 DT denotes each
T2465 2880-2886 NN denotes animal
T2466 2891-2892 CD denotes 1
T2467 2893-2895 NN denotes mg
T2468 2896-2899 NN denotes dox
T2469 2900-2903 IN denotes per
T2470 2904-2907 NN denotes day
T2471 2907-2908 . denotes .
T2473 2909-2912 DT denotes The
T2474 2926-2932 NN denotes animal
T2475 2913-2920 JJ denotes average
T2476 2921-2923 CD denotes 25
T2477 2924-2925 NN denotes g
T2478 2923-2924 HYPH denotes -
T2479 2943-2951 VBD denotes received
T2480 2933-2942 RB denotes therefore
T2481 2952-2954 CD denotes 40
T2482 2955-2957 NN denotes μg
T2483 2958-2960 IN denotes of
T2484 2961-2964 NN denotes dox
T2485 2965-2968 IN denotes per
T2486 2969-2973 NN denotes gram
T2487 2979-2985 NN denotes weight
T2488 2974-2978 NN denotes body
R1427 T2158 T2159 amod Pronuclear,Injection
R1428 T2160 T2159 punct ", ",Injection
R1429 T2161 T2159 conj Screening,Injection
R1430 T2162 T2161 prep of,Screening
R1431 T2163 T2162 pobj Founders,of
R1432 T2164 T2161 punct ", ",Screening
R1433 T2165 T2161 cc and,Screening
R1434 T2166 T2161 conj Maintenance,Screening
R1435 T2167 T2166 prep of,Maintenance
R1436 T2168 T2169 det the,Lines
R1437 T2169 T2167 pobj Lines,of
R1438 T2171 T2172 det The,vector
R1439 T2172 T2182 nsubjpass vector,linearized
R1440 T2173 T2174 compound moPrP,ind
R1441 T2174 T2172 compound ind,vector
R1442 T2175 T2174 punct -,ind
R1443 T2176 T2174 compound tetP,ind
R1444 T2177 T2174 punct -,ind
R1445 T2178 T2174 compound mo,ind
R1446 T2179 T2174 punct /,ind
R1447 T2180 T2174 compound huAPP695swe,ind
R1448 T2181 T2174 punct /,ind
R1449 T2183 T2182 auxpass was,linearized
R1450 T2184 T2182 cc and,linearized
R1451 T2185 T2186 det the,domain
R1452 T2186 T2188 nsubj domain,excised
R1453 T2187 T2186 compound pBluescript,domain
R1454 T2188 T2182 conj excised,linearized
R1455 T2189 T2188 prep by,excised
R1456 T2190 T2189 pobj digestion,by
R1457 T2191 T2190 prep with,digestion
R1458 T2192 T2191 pobj NotI,with
R1459 T2193 T2188 punct .,excised
R1460 T2195 T2196 det The,vector
R1461 T2196 T2198 nsubjpass vector,injected
R1462 T2197 T2196 amod purified,vector
R1463 T2199 T2198 auxpass was,injected
R1464 T2200 T2198 prep into,injected
R1465 T2201 T2202 det the,pronucleus
R1466 T2202 T2200 pobj pronucleus,into
R1467 T2203 T2202 prep of,pronucleus
R1468 T2204 T2205 amod fertilized,eggs
R1469 T2205 T2203 pobj eggs,of
R1470 T2206 T2205 prep from,eggs
R1471 T2207 T2208 nmod C57BL,C3HeJ
R1472 T2208 T2212 compound C3HeJ,matings
R1473 T2209 T2208 punct /,C3HeJ
R1474 T2210 T2208 nmod 6J,C3HeJ
R1475 T2211 T2208 punct ×,C3HeJ
R1476 T2212 T2206 pobj matings,from
R1477 T2213 T2212 compound F1,matings
R1478 T2214 T2198 punct .,injected
R1479 T2216 T2217 compound Founder,animals
R1480 T2217 T2218 nsubjpass animals,screened
R1481 T2219 T2218 auxpass were,screened
R1482 T2220 T2218 prep for,screened
R1483 T2221 T2222 det the,presence
R1484 T2222 T2220 pobj presence,for
R1485 T2223 T2222 prep of,presence
R1486 T2224 T2225 det the,transgene
R1487 T2225 T2223 pobj transgene,of
R1488 T2226 T2218 prep by,screened
R1489 T2227 T2228 nummod three,way
R1490 T2228 T2230 compound way,PCR
R1491 T2229 T2228 punct -,way
R1492 T2230 T2226 pobj PCR,by
R1493 T2231 T2218 advcl using,screened
R1494 T2232 T2233 det the,primers
R1495 T2233 T2231 dobj primers,using
R1496 T2234 T2233 nmod S36,primers
R1497 T2235 T2234 cc and,S36
R1498 T2236 T2237 compound PrP,AS
R1500 T2238 T2237 punct -,AS
R1501 T2239 T2237 compound S,AS
R1502 T2240 T2237 punct /,AS
R1503 T2241 T2237 compound PrP,AS
R1504 T2242 T2237 punct -,AS
R1505 T2243 T2233 acl described,primers
R1506 T2244 T2243 advmod below,described
R1507 T2245 T2218 punct .,screened
R1508 T2247 T2248 npadvmod Transgene,positive
R1509 T2248 T2250 amod positive,founders
R1510 T2249 T2248 punct -,positive
R1511 T2250 T2251 nsubjpass founders,bred
R1512 T2252 T2251 auxpass were,bred
R1513 T2253 T2251 prep to,bred
R1514 T2254 T2253 pobj animals,to
R1515 T2255 T2254 acl expressing,animals
R1516 T2256 T2257 det the,transactivator
R1517 T2257 T2255 dobj transactivator,expressing
R1518 T2258 T2257 compound tetracycline,transactivator
R1519 T2259 T2257 punct (,transactivator
R1520 T2260 T2257 appos tTA,transactivator
R1521 T2261 T2251 punct ),bred
R1522 T2262 T2251 prep under,bred
R1523 T2263 T2262 pobj control,under
R1524 T2264 T2263 prep of,control
R1525 T2265 T2266 det the,promoter
R1526 T2266 T2264 pobj promoter,of
R1527 T2267 T2268 nmod calcium,calmodulin
R1528 T2268 T2270 nmod calmodulin,IIα
R1529 T2269 T2268 punct -,calmodulin
R1530 T2270 T2266 nmod IIα,promoter
R1531 T2271 T2270 nmod kinase,IIα
R1532 T2272 T2266 punct (,promoter
R1533 T2273 T2266 nmod CaMKIIα,promoter
R1534 T2274 T2266 punct ),promoter
R1535 T2275 T2266 acl obtained,promoter
R1536 T2276 T2275 prep from,obtained
R1537 T2277 T2278 compound Jackson,Laboratory
R1538 T2278 T2276 pobj Laboratory,from
R1539 T2279 T2280 punct [,16
R1540 T2280 T2278 parataxis 16,Laboratory
R1541 T2281 T2280 punct ],16
R1542 T2282 T2283 punct (,Harbor
R1543 T2283 T2275 parataxis Harbor,obtained
R1544 T2284 T2283 compound Bar,Harbor
R1545 T2285 T2283 punct ", ",Harbor
R1546 T2286 T2283 npadvmod Maine,Harbor
R1547 T2287 T2283 punct ", ",Harbor
R1548 T2288 T2289 compound United,States
R1549 T2289 T2283 npadvmod States,Harbor
R1550 T2290 T2283 punct ;,Harbor
R1551 T2291 T2292 dep stock,1Mmay
R1552 T2292 T2283 dep 1Mmay,Harbor
R1553 T2293 T2291 punct #,stock
R1554 T2294 T2291 nummod 3010,stock
R1555 T2295 T2292 punct ;,1Mmay
R1556 T2296 T2292 dep B6,1Mmay
R1557 T2297 T2292 punct ;,1Mmay
R1558 T2298 T2292 nmod CBA,1Mmay
R1559 T2299 T2292 punct -,1Mmay
R1560 T2300 T2292 nmod TgN,1Mmay
R1561 T2301 T2292 punct [,1Mmay
R1562 T2302 T2292 nmod Camk2a,1Mmay
R1563 T2303 T2292 punct -,1Mmay
R1564 T2304 T2292 nmod tTA,1Mmay
R1565 T2305 T2292 punct ],1Mmay
R1566 T2306 T2283 punct ),Harbor
R1567 T2307 T2251 punct .,bred
R1568 T2309 T2310 det The,colony
R1569 T2310 T2311 nsubjpass colony,maintained
R1570 T2312 T2311 auxpass was,maintained
R1571 T2313 T2311 advmod thereafter,maintained
R1572 T2314 T2311 prep by,maintained
R1573 T2315 T2314 pcomp crossing,by
R1574 T2316 T2317 amod single,offspring
R1576 T2318 T2317 amod transgenic,offspring
R1577 T2319 T2317 nmod tTA,offspring
R1578 T2320 T2319 cc and,tTA
R1579 T2321 T2319 conj APP,tTA
R1580 T2322 T2315 prep for,crossing
R1581 T2323 T2322 pobj each,for
R1582 T2324 T2323 prep of,each
R1583 T2325 T2326 det the,lines
R1584 T2326 T2324 pobj lines,of
R1585 T2327 T2326 nummod four,lines
R1586 T2328 T2326 compound APP,lines
R1587 T2329 T2311 punct .,maintained
R1588 T2331 T2332 det All,mice
R1589 T2332 T2333 nsubjpass mice,provided
R1590 T2334 T2333 auxpass were,provided
R1591 T2335 T2336 amod fresh,food
R1592 T2336 T2333 dobj food,provided
R1593 T2337 T2336 cc and,food
R1594 T2338 T2336 conj water,food
R1595 T2339 T2340 advmod ad,libitum
R1596 T2340 T2333 advmod libitum,provided
R1597 T2341 T2333 punct .,provided
R1598 T2343 T2344 compound Animal,protocols
R1599 T2344 T2345 nsubjpass protocols,approved
R1600 T2346 T2345 auxpass were,approved
R1601 T2347 T2345 agent by,approved
R1602 T2348 T2349 det both,University
R1603 T2349 T2353 nmod University,Committees
R1604 T2350 T2349 det the,University
R1605 T2351 T2349 nmod Johns,University
R1606 T2352 T2349 nmod Hopkins,University
R1607 T2353 T2347 pobj Committees,by
R1608 T2354 T2349 cc and,University
R1609 T2355 T2356 det the,Institute
R1610 T2356 T2349 conj Institute,University
R1611 T2357 T2356 compound California,Institute
R1612 T2358 T2356 prep of,Institute
R1613 T2359 T2358 pobj Technology,of
R1614 T2360 T2361 nmod Institutional,Animal
R1615 T2361 T2353 nmod Animal,Committees
R1616 T2362 T2361 appos Care,Animal
R1617 T2363 T2362 cc and,Care
R1618 T2364 T2362 conj Use,Care
R1619 T2365 T2345 punct .,approved
R1622 T2408 T2409 compound Doxycycline,Administration
R1623 T2411 T2412 nsubjpass Doxycycline,administered
R1624 T2413 T2411 punct (,Doxycycline
R1625 T2414 T2411 appos dox,Doxycycline
R1626 T2415 T2411 punct ),Doxycycline
R1627 T2416 T2412 auxpass was,administered
R1628 T2417 T2412 prep through,administered
R1629 T2418 T2419 advmod commercially,available
R1630 T2419 T2420 amod available,chow
R1631 T2420 T2417 pobj chow,through
R1632 T2421 T2422 npadvmod dox,containing
R1633 T2422 T2420 amod containing,chow
R1634 T2423 T2422 punct -,containing
R1635 T2424 T2425 punct (,BioServ
R1636 T2425 T2412 parataxis BioServ,administered
R1637 T2426 T2425 punct ", ",BioServ
R1638 T2427 T2425 npadvmod Frenchtown,BioServ
R1639 T2428 T2425 punct ", ",BioServ
R1640 T2429 T2430 compound New,Jersey
R1641 T2430 T2425 npadvmod Jersey,BioServ
R1642 T2431 T2425 punct ", ",BioServ
R1643 T2432 T2433 compound United,States
R1644 T2433 T2425 npadvmod States,BioServ
R1645 T2434 T2425 punct ),BioServ
R1646 T2435 T2412 punct .,administered
R1647 T2437 T2438 det The,chow
R1648 T2438 T2439 nsubj chow,contained
R1649 T2439 T2440 ccomp contained,was
R1650 T2441 T2442 nummod 200,mg
R1651 T2442 T2439 dobj mg,contained
R1652 T2443 T2444 punct /,kg
R1653 T2444 T2442 prep kg,mg
R1654 T2445 T2442 prep of,mg
R1655 T2446 T2445 pobj antibiotic,of
R1656 T2447 T2440 punct ;,was
R1657 T2448 T2440 prep based,was
R1658 T2449 T2448 prep on,based
R1659 T2450 T2451 amod estimated,consumption
R1660 T2451 T2449 pobj consumption,on
R1661 T2452 T2451 prep of,consumption
R1662 T2453 T2454 nummod 5,g
R1663 T2454 T2452 pobj g,of
R1664 T2455 T2454 prep per,g
R1665 T2456 T2455 pobj mouse,per
R1666 T2457 T2454 prep per,g
R1667 T2458 T2457 pobj day,per
R1668 T2459 T2440 punct ", ",was
R1669 T2460 T2461 det the,dose
R1670 T2461 T2440 nsubj dose,was
R1671 T2462 T2461 amod expected,dose
R1672 T2463 T2461 prep to,dose
R1673 T2464 T2465 det each,animal
R1674 T2465 T2463 pobj animal,to
R1675 T2466 T2467 nummod 1,mg
R1676 T2467 T2468 compound mg,dox
R1677 T2468 T2440 attr dox,was
R1678 T2469 T2468 prep per,dox
R1679 T2470 T2469 pobj day,per
R1680 T2471 T2440 punct .,was
R1681 T2473 T2474 det The,animal
R1682 T2474 T2479 nsubj animal,received
R1683 T2475 T2474 amod average,animal
R1684 T2476 T2477 nummod 25,g
R1685 T2477 T2474 compound g,animal
R1686 T2478 T2477 punct -,g
R1687 T2480 T2479 advmod therefore,received
R1688 T2481 T2482 nummod 40,μg
R1689 T2482 T2479 dobj μg,received
R1690 T2483 T2482 prep of,μg
R1691 T2484 T2483 pobj dox,of
R1692 T2485 T2482 prep per,μg
R1693 T2486 T2487 compound gram,weight
R1694 T2487 T2485 pobj weight,per
R1695 T2488 T2487 compound body,weight
R1110 T1737 T1735 prep of,site
R1111 T1738 T1737 pobj exon,of
R1112 T1739 T1738 nummod 2,exon
R1113 T1740 T1741 punct (,TGC
R1114 T1741 T1735 parataxis TGC,site
R1115 T1742 T1743 dep forward,GG
R1116 T1743 T1741 dep GG,TGC
R1117 T1744 T1743 punct : ,GG
R1118 T1745 T1743 compound GCC,GG
R1119 T1746 T1743 compound GGA,GG
R1120 T1747 T1743 compound TCC,GG
R1121 T1748 T1743 compound GAT,GG
R1122 T1749 T1743 compound CAG,GG
R1123 T1750 T1743 compound CAG,GG
R1124 T1751 T1743 compound ACC,GG
R1125 T1752 T1743 compound GAT,GG
R1126 T1753 T1743 compound TCT,GG
R1127 T1754 T1741 punct ;,TGC
R1128 T1755 T1741 dep reverse,TGC
R1129 T1756 T1741 punct : ,TGC
R1130 T1757 T1741 compound GCC,TGC
R1131 T1758 T1741 compound GGT,TGC
R1132 T1759 T1741 compound ACC,TGC
R1133 T1760 T1741 compound ACT,TGC
R1134 T1761 T1741 compound AGG,TGC
R1135 T1762 T1741 compound AAG,TGC
R1136 T1763 T1741 compound GCA,TGC
R1137 T1764 T1741 compound GAA,TGC
R1138 T1765 T1741 punct ),TGC
R1140 T1768 T1769 det This,fragment
R1142 T1770 T1771 nummod 2,kb
R1143 T1771 T1769 compound kb,fragment
R1144 T1772 T1771 punct -,kb
R1145 T1773 T1769 compound intron,fragment
R1146 T1775 T1774 aux was,cloned
R1147 T1776 T1774 prep into,cloned
R1148 T1777 T1778 det a,vector
R1149 T1778 T1776 pobj vector,into
R1150 T1779 T1780 compound TA,cloning
R1151 T1780 T1778 compound cloning,vector
R1152 T1781 T1782 punct (,Invitrogen
R1153 T1782 T1778 parataxis Invitrogen,vector
R1154 T1783 T1782 punct ", ",Invitrogen
R1155 T1784 T1782 npadvmod Carlsbad,Invitrogen
R1156 T1785 T1782 punct ", ",Invitrogen
R1157 T1786 T1782 npadvmod California,Invitrogen
R1158 T1787 T1782 punct ", ",Invitrogen
R1159 T1788 T1789 compound United,States
R1160 T1789 T1782 npadvmod States,Invitrogen
R1161 T1790 T1782 punct ),Invitrogen
R1162 T1791 T1774 punct ", ",cloned
R1163 T1792 T1793 advmod then,excised
R1164 T1793 T1774 conj excised,cloned
R1165 T1794 T1793 prep by,excised
R1166 T1795 T1796 compound Asp718,digestion
R1167 T1796 T1794 pobj digestion,by
R1168 T1797 T1793 cc and,excised
R1169 T1798 T1793 conj ligated,excised
R1170 T1799 T1798 prep to,ligated
R1171 T1800 T1801 det the,fragment
R1173 T1802 T1803 nummod 6.8,kb
R1174 T1803 T1801 compound kb,fragment
R1175 T1804 T1803 punct -,kb
R1176 T1805 T1801 compound Asp718,fragment
R1177 T1806 T1801 prep of,fragment
R1178 T1807 T1806 pobj moPrP.XhoI,of
R1179 T1808 T1801 acl containing,fragment
R1180 T1809 T1808 dobj exon,containing
R1181 T1810 T1809 nummod 2,exon
R1182 T1811 T1809 punct ", ",exon
R1183 T1812 T1809 conj exon,exon
R1184 T1813 T1812 nummod 3,exon
R1185 T1814 T1812 punct ", ",exon
R1186 T1815 T1816 det the,UTR
R1187 T1816 T1812 conj UTR,exon
R1188 T1817 T1816 nummod 3,UTR
R1189 T1818 T1817 punct ′,3
R1190 T1819 T1816 punct ", ",UTR
R1191 T1820 T1816 cc and,UTR
R1192 T1821 T1816 conj pBluescript,UTR
R1193 T1822 T1823 aux to,generate
R1194 T1823 T1774 advcl generate,cloned
R1195 T1824 T1825 det an,vector
R1196 T1825 T1823 dobj vector,generate
R1197 T1826 T1825 amod intermediate,vector
R1198 T1827 T1823 prep with,generate
R1199 T1828 T1829 det all,exons
R1200 T1829 T1827 pobj exons,with
R1201 T1830 T1829 nummod three,exons
R1202 T1831 T1829 cc and,exons
R1203 T1832 T1833 det a,intron
R1204 T1833 T1829 conj intron,exons
R1205 T1834 T1833 amod central,intron
R1206 T1835 T1829 cc but,exons
R1207 T1836 T1837 det no,promoter
R1208 T1837 T1829 conj promoter,exons
R1209 T1838 T1774 punct .,cloned
R1210 T1840 T1841 det This,vector
R1211 T1841 T1842 nsubjpass vector,opened
R1212 T1843 T1842 auxpass was,opened
R1213 T1844 T1842 advmod then,opened
R1214 T1845 T1842 prep at,opened
R1215 T1846 T1847 det the,site
R1216 T1847 T1845 pobj site,at
R1217 T1848 T1847 compound BamHI,site
R1218 T1849 T1847 acl introduced,site
R1219 T1850 T1849 agent by,introduced
R1220 T1851 T1852 det the,primer
R1221 T1852 T1850 pobj primer,by
R1222 T1853 T1852 compound intron,primer
R1223 T1854 T1852 compound cloning,primer
R1224 T1855 T1842 punct ", ",opened
R1225 T1856 T1842 cc and,opened
R1226 T1857 T1842 conj ligated,opened
R1227 T1858 T1857 prep to,ligated
R1228 T1859 T1860 det the,fragment
R1229 T1860 T1858 pobj fragment,to
R1230 T1861 T1862 nummod 0.5,kb
R1231 T1862 T1860 compound kb,fragment
R1232 T1863 T1862 punct -,kb
R1233 T1864 T1865 compound BamHI,cut
R1234 T1865 T1860 compound cut,fragment
R1235 T1866 T1865 punct -,cut
R1236 T1867 T1868 compound tetracycline,promoter
R1237 T1868 T1860 compound promoter,fragment
R1238 T1869 T1842 punct .,opened
R1239 T1871 T1872 det This,ligation
R1240 T1872 T1873 nsubj ligation,generated
R1241 T1874 T1875 det a,vector
R1242 T1875 T1873 dobj vector,generated
R1243 T1876 T1877 nummod 9.3,kb
R1244 T1877 T1875 compound kb,vector
R1245 T1878 T1877 punct -,kb
R1246 T1879 T1875 acl encoding,vector
R1247 T1880 T1881 det the,promoter
R1248 T1881 T1879 dobj promoter,encoding
R1249 T1882 T1881 compound tetracycline,promoter
R1250 T1883 T1881 prep from,promoter
R1251 T1884 T1883 pobj pTetSplice,from
R1252 T1885 T1879 prep with,encoding
R1253 T1886 T1887 nummod two,exons
R1254 T1887 T1885 pobj exons,with
R1255 T1888 T1887 punct ", ",exons
R1256 T1889 T1890 nummod one,intron
R1257 T1890 T1887 conj intron,exons
R1258 T1891 T1890 punct ", ",intron
R1259 T1892 T1890 cc and,intron
R1260 T1893 T1894 det the,UTR
R1261 T1894 T1890 conj UTR,intron
R1262 T1895 T1894 amod original,UTR
R1263 T1896 T1894 nummod 3,UTR
R1264 T1897 T1896 punct ′,3
R1265 T1898 T1894 prep of,UTR
R1266 T1899 T1900 det the,vector
R1267 T1900 T1898 pobj vector,of
R1268 T1901 T1900 compound moPrP.XhoI,vector
R1269 T1902 T1887 punct ", ",exons
R1270 T1903 T1904 advmod all,carried
R1271 T1904 T1887 acl carried,exons
R1272 T1905 T1904 prep in,carried
R1273 T1906 T1907 det the,vector
R1274 T1907 T1905 pobj vector,in
R1275 T1908 T1909 compound pBluescript,cloning
R1276 T1909 T1907 compound cloning,vector
R1277 T1910 T1873 punct .,generated
R1278 T1912 T1913 nsubj We,incorporated
R1279 T1913 T1914 ccomp incorporated,generated
R1280 T1915 T1916 det the,mutations
R1281 T1916 T1913 dobj mutations,incorporated
R1282 T1917 T1916 amod Swedish,mutations
R1283 T1918 T1919 punct (,571NL
R1284 T1919 T1917 parataxis 571NL,Swedish
R1285 T1920 T1919 compound KM570,571NL
R1286 T1921 T1919 punct /,571NL
R1287 T1922 T1919 punct ),571NL
R1288 T1923 T1917 cc and,Swedish
R1289 T1924 T1917 conj Indiana,Swedish
R1290 T1925 T1926 punct (,V617F
R1291 T1926 T1924 parataxis V617F,Indiana
R1292 T1927 T1926 punct ),V617F
R1293 T1928 T1913 prep into,incorporated
R1294 T1929 T1930 det the,cDNA
R1295 T1930 T1928 pobj cDNA,into
R1296 T1931 T1932 compound mo,huAPP695
R1297 T1932 T1930 compound huAPP695,cDNA
R1298 T1933 T1932 punct /,huAPP695
R1299 T1934 T1930 punct (,cDNA
R1300 T1935 T1930 prep in,cDNA
R1301 T1936 T1937 compound BS,KS
R1302 T1937 T1935 pobj KS,in
R1303 T1938 T1937 punct -,KS
R1304 T1939 T1913 punct ),incorporated
R1305 T1940 T1913 prep by,incorporated
R1306 T1941 T1940 pobj PCR,by
R1307 T1942 T1913 advcl using,incorporated
R1308 T1943 T1944 det a,strategy
R1309 T1944 T1942 dobj strategy,using
R1310 T1945 T1946 nummod four,primer
R1311 T1946 T1944 compound primer,strategy
R1312 T1947 T1946 punct -,primer
R1313 T1948 T1914 punct : ,generated
R1314 T1949 T1914 advmod first,generated
R1315 T1950 T1914 punct ", ",generated
R1316 T1951 T1952 nummod two,products
R1317 T1952 T1914 nsubjpass products,generated
R1318 T1953 T1954 advmod partially,overlapping
R1319 T1954 T1952 amod overlapping,products
R1320 T1955 T1914 auxpass were,generated
R1321 T1956 T1914 prep in,generated
R1322 T1957 T1958 amod separate,reactions
R1323 T1958 T1956 pobj reactions,in
R1324 T1959 T1914 advcl using,generated
R1325 T1960 T1959 dobj primers,using
R1326 T1961 T1962 dep that,encode
R1327 T1962 T1960 relcl encode,primers
R1328 T1963 T1964 det the,mutations
R1329 T1964 T1962 dobj mutations,encode
R1330 T1965 T1964 amod desired,mutations
R1331 T1966 T1967 punct (,M13
R1332 T1967 T1962 parataxis M13,encode
R1333 T1968 T1969 compound Swedish,forward
R1334 T1969 T1970 dep forward,CCG
R1335 T1970 T1983 dep CCG,C
R1336 T1971 T1970 punct : ,CCG
R1337 T1972 T1970 compound GGA,CCG
R1338 T1973 T1970 compound GAT,CCG
R1339 T1974 T1970 compound CTC,CCG
R1340 T1975 T1970 compound TGA,CCG
R1341 T1976 T1970 compound AGT,CCG
R1342 T1977 T1970 compound GAA,CCG
R1343 T1978 T1970 compound TCT,CCG
R1344 T1979 T1970 compound GGA,CCG
R1345 T1980 T1970 compound TGC,CCG
R1346 T1981 T1970 compound AGA,CCG
R1347 T1982 T1970 compound ATT,CCG
R1348 T1983 T1967 dep C,M13
R1349 T1984 T1983 punct /,C
R1350 T1985 T1986 compound Indiana,reverse
R1351 T1986 T1983 dep reverse,C
R1352 T1987 T1983 punct : ,C
R1353 T1988 T1983 compound GGG,C
R1354 T1989 T1983 compound TGA,C
R1355 T1990 T1983 compound TGA,C
R1356 T1991 T1983 compound AAA,C
R1357 T1992 T1983 compound TCA,C
R1358 T1993 T1983 compound CGG,C
R1359 T1994 T1983 compound TTG,C
R1360 T1995 T1967 punct ;,M13
R1361 T1996 T1997 compound Indiana,forward
R1362 T1997 T1967 dep forward,M13
R1363 T1998 T1967 punct : ,M13
R1364 T1999 T1967 compound CAA,M13
R1365 T2000 T1967 compound CCG,M13
R1366 T2001 T1967 compound TGA,M13
R1367 T2002 T1967 compound TTT,M13
R1368 T2003 T1967 compound TCA,M13
R1369 T2004 T1967 compound TCA,M13
R1370 T2005 T1967 compound CCC,M13
R1371 T2006 T1967 compound TGG,M13
R1372 T2007 T1967 punct /,M13
R1373 T2008 T1967 advmod reverse,M13
R1374 T2009 T1967 punct ),M13
R1375 T2010 T1914 punct .,generated
R1376 T2012 T2013 det The,products
R1377 T2013 T2016 nsubjpass products,ligated
R1378 T2014 T2013 nummod two,products
R1379 T2015 T2013 compound PCR,products
R1380 T2017 T2016 auxpass were,ligated
R1381 T2018 T2016 punct ", ",ligated
R1382 T2019 T2016 dep digested,ligated
R1383 T2020 T2016 prep with,ligated
R1384 T2021 T2020 pobj BglII,with
R1385 T2022 T2021 cc and,BglII
R1386 T2023 T2021 conj ApaI,BglII
R1387 T2024 T2016 cc and,ligated
R1388 T2025 T2016 conj cloned,ligated
R1389 T2026 T2025 advmod back,cloned
R1390 T2027 T2025 prep into,cloned
R1391 T2028 T2029 det the,vector
R1392 T2029 T2027 pobj vector,into
R1393 T2030 T2029 amod original,vector
R1394 T2031 T2032 compound mo,KS
R1396 T2033 T2032 punct /,KS
R1397 T2034 T2032 compound huAPP695,KS
R1398 T2035 T2032 punct -,KS
R1399 T2036 T2032 compound BS,KS
R1400 T2037 T2032 punct -,KS
R1401 T2038 T2016 punct .,ligated
R1402 T2040 T2041 advmod Finally,subcloned
R1403 T2042 T2041 punct ", ",subcloned
R1404 T2043 T2044 det the,ind
R1405 T2044 T2041 nsubjpass ind,subcloned
R1406 T2045 T2044 amod new,ind
R1407 T2046 T2044 compound APP695swe,ind
R1408 T2047 T2044 punct /,ind
R1409 T2048 T2041 auxpass was,subcloned
R1410 T2049 T2041 prep into,subcloned
R1411 T2050 T2051 det the,site
R1412 T2051 T2049 pobj site,into
R1413 T2052 T2051 compound XhoI,site
R1414 T2053 T2051 prep of,site
R1415 T2054 T2055 det the,vector
R1416 T2055 T2053 pobj vector,of
R1417 T2056 T2057 compound moPrP,tetP
R1418 T2057 T2055 compound tetP,vector
R1419 T2058 T2057 punct -,tetP
R1420 T2059 T2041 prep from,subcloned
R1421 T2060 T2059 pcomp above,from
R1422 T2061 T2062 aux to,complete
R1423 T2062 T2041 advcl complete,subcloned
R1424 T2063 T2064 det the,construct
R1425 T2064 T2062 dobj construct,complete
R1426 T2065 T2041 punct .,subcloned
R1499 T2237 T2234 conj AS,S36
R1575 T2317 T2315 dobj offspring,crossing
R1141 T1769 T1774 nsubj fragment,cloned
R1172 T1801 T1799 pobj fragment,to
R1395 T2032 T2029 compound KS,vector

2_test

Id Subject Object Predicate Lexical cue
16279840-8939850-86242755 2172-2174 8939850 denotes 16
T66109 2172-2174 8939850 denotes 16

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T1473 10-14 SO_EXT:0000147 denotes exon
T1474 18-25 SO:0001030 denotes forward
T1475 67-74 SO:0001031 denotes reverse
T1476 122-123 CHEBI_SO_EXT:base denotes b
T1477 124-130 SO_EXT:0000188 denotes intron
T1478 144-150 SO_EXT:sequence_cloning_process denotes cloned
T1479 161-168 SO_EXT:sequence_cloning_process denotes cloning
T1480 169-175 SO_EXT:0000440 denotes vector
T1481 232-239 SO_EXT:sequence_deletion_process denotes excised
T1482 264-271 GO:0006266 denotes ligated
T1483 284-285 CHEBI_SO_EXT:base denotes b
T1484 305-310 PR_EXT:P04925 denotes moPrP
T1485 327-331 SO_EXT:0000147 denotes exon
T1486 335-339 SO_EXT:0000147 denotes exon
T1487 347-353 SO_EXT:0000205 denotes 3′ UTR
T1488 399-405 SO_EXT:0000440 denotes vector
T1489 421-426 SO_EXT:0000147 denotes exons
T1490 441-447 SO_EXT:0000188 denotes intron
T1491 455-463 SO_EXT:0000167 denotes promoter
T1492 470-476 SO_EXT:0000440 denotes vector
T1493 500-505 PR_EXT:P23940 denotes BamHI
T1494 529-535 SO_EXT:0000188 denotes intron
T1495 536-543 SO_EXT:sequence_cloning_process denotes cloning
T1496 544-550 SO_EXT:0000112 denotes primer
T1497 556-563 GO:0006266 denotes ligated
T1498 576-577 CHEBI_SO_EXT:base denotes b
T1499 578-583 PR_EXT:P23940 denotes BamHI
T1500 584-587 MOP:0000780 denotes cut
T1501 588-600 CHEBI:27902 denotes tetracycline
T1502 601-609 SO_EXT:0000167 denotes promoter
T1503 625-633 GO:0006266 denotes ligation
T1504 651-652 CHEBI_SO_EXT:base denotes b
T1505 653-659 SO_EXT:0000440 denotes vector
T1506 660-668 SO_EXT:sequence_coding_function denotes encoding
T1507 673-685 CHEBI:27902 denotes tetracycline
T1508 686-694 SO_EXT:0000167 denotes promoter
T1509 720-725 SO_EXT:0000147 denotes exons
T1510 731-737 SO_EXT:0000188 denotes intron
T1511 756-762 SO_EXT:0000205 denotes 3′ UTR
T1512 770-775 PR_EXT:P04925 denotes moPrP
T1513 781-787 SO_EXT:0000440 denotes vector
T1514 820-827 SO_EXT:sequence_cloning_process denotes cloning
T1515 828-834 SO_EXT:0000440 denotes vector
T1516 898-907 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T1517 917-919 NCBITaxon:10088 denotes mo
T1518 929-933 SO_EXT:cDNA denotes cDNA
T1519 965-971 SO_EXT:0000112 denotes primer
T1520 1067-1074 SO_EXT:0000112 denotes primers
T1521 1080-1086 SO_EXT:sequence_coding_function denotes encode
T1522 1099-1108 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T1523 1118-1125 SO:0001030 denotes forward
T1524 1183-1190 SO:0001031 denotes reverse
T1525 1231-1238 SO:0001030 denotes forward
T1526 1276-1283 SO:0001031 denotes reverse
T1527 1294-1306 SO_EXT:0000006 denotes PCR products
T1528 1312-1319 GO:0006266 denotes ligated
T1529 1354-1360 SO_EXT:sequence_cloning_process denotes cloned
T1530 1384-1386 NCBITaxon:10088 denotes mo
T1531 1402-1408 SO_EXT:0000440 denotes vector
T1532 1481-1486 PR_EXT:P04925 denotes moPrP
T1533 1492-1498 SO_EXT:0000440 denotes vector
T1534 1526-1535 SO_EXT:engineered_biological_sequence denotes construct
T2122 1538-1548 GO:0045120 denotes Pronuclear
T2123 1616-1621 PR_EXT:P04925 denotes moPrP
T2124 1627-1629 NCBITaxon:10088 denotes mo
T2125 1646-1652 SO_EXT:0000440 denotes vector
T2126 1657-1667 SO_EXT:sequence_linearization denotes linearized
T2127 1695-1702 SO_EXT:sequence_deletion_process denotes excised
T2128 1740-1746 SO_EXT:0000440 denotes vector
T2129 1769-1779 GO:0045120 denotes pronucleus
T2130 1783-1793 GO:0009566 denotes fertilized
T2131 1794-1798 CL:0000025 denotes eggs
T2132 1824-1831 GO:0007618 denotes matings
T2133 1841-1848 NCBITaxon:33208 denotes animals
T2134 1887-1896 SO_EXT:0000902 denotes transgene
T2135 1932-1935 PR_EXT:000001886 denotes PrP
T2136 1938-1941 PR_EXT:000001886 denotes PrP
T2137 1945-1952 SO_EXT:0000112 denotes primers
T2138 1970-1979 SO_EXT:0000902 denotes Transgene
T2139 2003-2007 GO_EXT:breeding denotes bred
T2140 2011-2018 NCBITaxon:33208 denotes animals
T2142 2034-2046 CHEBI:27902 denotes tetracycline
T2143 2074-2081 GO:0065007 denotes control
T2144 2089-2096 CHEBI_EXT:calcium denotes calcium
T2145 2089-2114 GO_EXT:0004683 denotes calcium-calmodulin kinase
T2146 2089-2118 PR_EXT:000003199 denotes calcium-calmodulin kinase IIα
T2147 2120-2127 PR_EXT:000003199 denotes CaMKIIα
T2148 2129-2137 SO_EXT:0000167 denotes promoter
T2149 2236-2242 PR_EXT:000003199 denotes Camk2a
T2150 2311-2321 SO_EXT:transgenic_entity denotes transgenic
T2151 2330-2333 PR_EXT:000004168 denotes APP
T2152 2365-2368 PR_EXT:000004168 denotes APP
T2153 2380-2384 NCBITaxon:10088 denotes mice
T2154 2405-2409 CHEBI_EXT:33290 denotes food
T2155 2414-2419 CHEBI:15377 denotes water
T2156 2432-2438 NCBITaxon:33208 denotes Animal
T2157 2557-2563 NCBITaxon:33208 denotes Animal
T2390 2590-2601 CHEBI:50845 denotes Doxycycline
T2391 2617-2628 CHEBI:50845 denotes Doxycycline
T2392 2630-2633 CHEBI:50845 denotes dox
T2393 2683-2686 CHEBI:50845 denotes dox
T2394 2698-2702 CHEBI_EXT:33290 denotes chow
T2395 2757-2761 CHEBI_EXT:33290 denotes chow
T2396 2785-2795 CHEBI_EXT:33282 denotes antibiotic
T2397 2816-2827 GO:0007631 denotes consumption
T2398 2839-2844 NCBITaxon:10088 denotes mouse
T2399 2880-2886 NCBITaxon:33208 denotes animal
T2400 2896-2899 CHEBI:50845 denotes dox
T2401 2926-2932 NCBITaxon:33208 denotes animal
T2402 2961-2964 CHEBI:50845 denotes dox
T2403 2974-2978 UBERON_EXT:body denotes body
T2141 2019-2029 GO:0010467 denotes expressing

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T1392 10-14 SO:0000147 denotes exon
T1393 18-25 SO:0001030 denotes forward
T1394 67-74 SO:0001031 denotes reverse
T1395 124-130 SO:0000188 denotes intron
T1396 169-175 SO:0000440 denotes vector
T1397 264-271 GO:0006266 denotes ligated
T1398 305-310 PR:P04925 denotes moPrP
T1399 327-331 SO:0000147 denotes exon
T1400 335-339 SO:0000147 denotes exon
T1401 347-353 SO:0000205 denotes 3′ UTR
T1402 399-405 SO:0000440 denotes vector
T1403 421-426 SO:0000147 denotes exons
T1404 441-447 SO:0000188 denotes intron
T1405 455-463 SO:0000167 denotes promoter
T1406 470-476 SO:0000440 denotes vector
T1407 500-505 PR:P23940 denotes BamHI
T1408 529-535 SO:0000188 denotes intron
T1409 544-550 SO:0000112 denotes primer
T1410 556-563 GO:0006266 denotes ligated
T1411 578-583 PR:P23940 denotes BamHI
T1412 584-587 MOP:0000780 denotes cut
T1413 588-600 CHEBI:27902 denotes tetracycline
T1414 601-609 SO:0000167 denotes promoter
T1415 625-633 GO:0006266 denotes ligation
T1416 653-659 SO:0000440 denotes vector
T1417 673-685 CHEBI:27902 denotes tetracycline
T1418 686-694 SO:0000167 denotes promoter
T1419 720-725 SO:0000147 denotes exons
T1420 731-737 SO:0000188 denotes intron
T1421 756-762 SO:0000205 denotes 3′ UTR
T1422 770-775 PR:P04925 denotes moPrP
T1423 781-787 SO:0000440 denotes vector
T1424 828-834 SO:0000440 denotes vector
T1425 917-919 NCBITaxon:10088 denotes mo
T1426 965-971 SO:0000112 denotes primer
T1427 1067-1074 SO:0000112 denotes primers
T1428 1118-1125 SO:0001030 denotes forward
T1429 1183-1190 SO:0001031 denotes reverse
T1430 1231-1238 SO:0001030 denotes forward
T1431 1276-1283 SO:0001031 denotes reverse
T1432 1294-1306 SO:0000006 denotes PCR products
T1433 1312-1319 GO:0006266 denotes ligated
T1434 1384-1386 NCBITaxon:10088 denotes mo
T1435 1402-1408 SO:0000440 denotes vector
T1436 1481-1486 PR:P04925 denotes moPrP
T1437 1492-1498 SO:0000440 denotes vector
T2092 1538-1548 GO:0045120 denotes Pronuclear
T2093 1616-1621 PR:P04925 denotes moPrP
T2094 1627-1629 NCBITaxon:10088 denotes mo
T2095 1646-1652 SO:0000440 denotes vector
T2096 1740-1746 SO:0000440 denotes vector
T2097 1769-1779 GO:0045120 denotes pronucleus
T2098 1783-1793 GO:0009566 denotes fertilized
T2099 1794-1798 CL:0000025 denotes eggs
T2100 1824-1831 GO:0007618 denotes matings
T2101 1841-1848 NCBITaxon:33208 denotes animals
T2102 1887-1896 SO:0000902 denotes transgene
T2103 1932-1935 PR:000001886 denotes PrP
T2104 1938-1941 PR:000001886 denotes PrP
T2105 1945-1952 SO:0000112 denotes primers
T2106 1970-1979 SO:0000902 denotes Transgene
T2107 2011-2018 NCBITaxon:33208 denotes animals
T2108 2019-2029 GO:0010467 denotes expressing
T2109 2034-2046 CHEBI:27902 denotes tetracycline
T2110 2074-2081 GO:0065007 denotes control
T2111 2089-2118 PR:000003199 denotes calcium-calmodulin kinase IIα
T2112 2120-2127 PR:000003199 denotes CaMKIIα
T2113 2129-2137 SO:0000167 denotes promoter
T2114 2236-2242 PR:000003199 denotes Camk2a
T2115 2330-2333 PR:000004168 denotes APP
T2116 2365-2368 PR:000004168 denotes APP
T2117 2380-2384 NCBITaxon:10088 denotes mice
T2118 2405-2409 CHEBI:33290 denotes food
T2119 2414-2419 CHEBI:15377 denotes water
T2120 2432-2438 NCBITaxon:33208 denotes Animal
T2121 2557-2563 NCBITaxon:33208 denotes Animal
T2373 2590-2601 CHEBI:50845 denotes Doxycycline
T2374 2617-2628 CHEBI:50845 denotes Doxycycline
T2375 2630-2633 CHEBI:50845 denotes dox
T2376 2683-2686 CHEBI:50845 denotes dox
T2377 2698-2702 CHEBI:33290 denotes chow
T2378 2757-2761 CHEBI:33290 denotes chow
T2379 2785-2795 CHEBI:33282 denotes antibiotic
T2380 2816-2827 GO:0007631 denotes consumption
T2381 2839-2844 NCBITaxon:10088 denotes mouse
T2382 2880-2886 NCBITaxon:33208 denotes animal
T2383 2896-2899 CHEBI:50845 denotes dox
T2384 2926-2932 NCBITaxon:33208 denotes animal
T2385 2961-2964 CHEBI:50845 denotes dox