PMC:1283364 / 21074-48151 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T6511	8-10	PRP	denotes	We
T6512	11-15	VBD	denotes	used
T6513	16-19	DT	denotes	the
T6514	38-44	NN	denotes	system
T6515	20-23	NN	denotes	tet
T6516	24-27	NN	denotes	off
T6517	23-24	HYPH	denotes	-
T6518	28-37	NN	denotes	transgene
T6519	45-47	TO	denotes	to
T6520	48-55	VB	denotes	express
T6521	56-57	DT	denotes	a
T6522	72-79	NN	denotes	version
T6523	58-64	JJ	denotes	double
T6524	65-71	NN	denotes	mutant
T6525	80-82	IN	denotes	of
T6526	83-91	JJ	denotes	chimeric
T6527	95-103	NN	denotes	huAPP695
T6528	92-94	NN	denotes	mo
T6529	94-95	HYPH	denotes	/
T6530	104-105	-LRB-	denotes	(
T6531	105-108	NN	denotes	swe
T6532	113-118	NN	denotes	KM570
T6533	108-109	HYPH	denotes	/
T6534	109-112	NN	denotes	ind
T6535	118-120	,	denotes	,
T6536	120-125	NN	denotes	571NL
T6537	125-127	,	denotes	,
T6538	127-130	CC	denotes	and
T6539	131-136	NN	denotes	V617F
T6540	136-137	-RRB-	denotes	)
T6541	138-142	IN	denotes	from
T6542	143-144	DT	denotes	a
T6543	169-177	NN	denotes	promoter
T6544	145-157	NN	denotes	tetracycline
T6545	158-168	JJ	denotes	responsive
T6546	157-158	HYPH	denotes	-
T6547	178-179	-LRB-	denotes	[
T6548	182-184	CD	denotes	13
T6549	179-181	CD	denotes	12
T6550	181-182	,	denotes	,
T6551	184-185	-RRB-	denotes	]
T6552	185-186	.	denotes	.
T6553	186-327	sentence	denotes	Transgenic APP expression was activated by crossing the APPswe/ind mice to animals producing tTA under control of the CaMKIIα promoter [16].
T6554	187-197	JJ	denotes	Transgenic
T6555	198-201	NN	denotes	APP
T6556	202-212	NN	denotes	expression
T6557	217-226	VBN	denotes	activated
T6558	213-216	VBD	denotes	was
T6559	227-229	IN	denotes	by
T6560	230-238	VBG	denotes	crossing
T6561	239-242	DT	denotes	the
T6562	254-258	NNS	denotes	mice
T6563	243-249	NN	denotes	APPswe
T6564	250-253	NN	denotes	ind
T6565	249-250	HYPH	denotes	/
T6566	259-261	IN	denotes	to
T6567	262-269	NNS	denotes	animals
T6568	270-279	VBG	denotes	producing
T6569	280-283	NN	denotes	tTA
T6570	284-289	IN	denotes	under
T6571	290-297	NN	denotes	control
T6572	298-300	IN	denotes	of
T6573	301-304	DT	denotes	the
T6574	313-321	NN	denotes	promoter
T6575	305-312	NN	denotes	CaMKIIα
T6576	322-323	-LRB-	denotes	[
T6577	323-325	CD	denotes	16
T6578	325-326	-RRB-	denotes	]
T6579	326-327	.	denotes	.
T6580	327-521	sentence	denotes	After initial screening of founders, we identified four lines of tet-APPswe/ind mice that produced very high levels of transgene product in offspring coexpressing tTA (Figures 1 and Figure S1).
T6581	328-333	IN	denotes	After
T6582	368-378	VBD	denotes	identified
T6583	334-341	JJ	denotes	initial
T6584	342-351	NN	denotes	screening
T6585	352-354	IN	denotes	of
T6586	355-363	NNS	denotes	founders
T6587	363-365	,	denotes	,
T6588	365-367	PRP	denotes	we
T6589	379-383	CD	denotes	four
T6590	384-389	NNS	denotes	lines
T6591	390-392	IN	denotes	of
T6592	393-396	NN	denotes	tet
T6593	404-407	NN	denotes	ind
T6594	396-397	HYPH	denotes	-
T6595	397-403	NN	denotes	APPswe
T6596	403-404	HYPH	denotes	/
T6597	408-412	NNS	denotes	mice
T6598	413-417	WDT	denotes	that
T6599	418-426	VBD	denotes	produced
T6600	427-431	RB	denotes	very
T6601	432-436	JJ	denotes	high
T6602	437-443	NNS	denotes	levels
T6603	444-446	IN	denotes	of
T6604	447-456	NN	denotes	transgene
T6605	457-464	NN	denotes	product
T6606	465-467	IN	denotes	in
T6607	468-477	NN	denotes	offspring
T6608	478-490	VBG	denotes	coexpressing
T6609	491-494	NN	denotes	tTA
T6610	495-496	-LRB-	denotes	(
T6611	496-503	NNS	denotes	Figures
T6612	504-505	CD	denotes	1
T6613	506-509	CC	denotes	and
T6614	510-516	NN	denotes	Figure
T6615	517-519	NN	denotes	S1
T6616	519-520	-RRB-	denotes	)
T6617	520-521	.	denotes	.
T6618	521-772	sentence	denotes	Compared to a standard APP transgenic line used for previous amyloid studies by our laboratory (line C3–3; [15,26,27]), we estimated that the four controllable lines produce transgenic APP protein at 10- to 30-fold over endogenous levels (Figure S1).
T6619	522-530	VBN	denotes	Compared
T6620	645-654	VBD	denotes	estimated
T6621	531-533	IN	denotes	to
T6622	534-535	DT	denotes	a
T6623	560-564	NN	denotes	line
T6624	536-544	JJ	denotes	standard
T6625	545-548	NN	denotes	APP
T6626	549-559	JJ	denotes	transgenic
T6627	565-569	VBN	denotes	used
T6628	570-573	IN	denotes	for
T6629	574-582	JJ	denotes	previous
T6630	591-598	NNS	denotes	studies
T6631	583-590	NN	denotes	amyloid
T6632	599-601	IN	denotes	by
T6633	602-605	PRP$	denotes	our
T6634	606-616	NN	denotes	laboratory
T6635	617-618	-LRB-	denotes	(
T6636	636-638	CD	denotes	27
T6637	618-622	NN	denotes	line
T6638	623-625	NN	denotes	C3
T6639	625-626	HYPH	denotes	–
T6640	626-627	CD	denotes	3
T6641	627-628	:	denotes	;
T6642	629-630	-LRB-	denotes	[
T6643	630-632	CD	denotes	15
T6644	632-633	,	denotes	,
T6645	633-635	CD	denotes	26
T6646	635-636	,	denotes	,
T6647	638-639	-RRB-	denotes	]
T6648	639-640	-RRB-	denotes	)
T6649	640-642	,	denotes	,
T6650	642-644	PRP	denotes	we
T6651	655-659	IN	denotes	that
T6652	688-695	VBP	denotes	produce
T6653	660-663	DT	denotes	the
T6654	682-687	NNS	denotes	lines
T6655	664-668	CD	denotes	four
T6656	669-681	JJ	denotes	controllable
T6657	696-706	JJ	denotes	transgenic
T6658	711-718	NN	denotes	protein
T6659	707-710	NN	denotes	APP
T6660	719-721	IN	denotes	at
T6661	722-724	CD	denotes	10
T6662	724-725	HYPH	denotes	-
T6663	726-728	IN	denotes	to
T6664	729-736	RB	denotes	30-fold
T6665	737-741	IN	denotes	over
T6666	742-752	JJ	denotes	endogenous
T6667	753-759	NNS	denotes	levels
T6668	760-761	-LRB-	denotes	(
T6669	768-770	NN	denotes	S1
T6670	761-767	NN	denotes	Figure
T6671	770-771	-RRB-	denotes	)
T6672	771-772	.	denotes	.
T6673	772-1033	sentence	denotes	This estimate was confirmed by direct comparison of APP levels in nontransgenic and tet-off APP mice using an antibody that recognizes both endogenous APP (and amyloid precursor-like protein 2) and the transgenic protein (monoclonal antibody 22C11; Figure 1D).
T6674	773-777	DT	denotes	This
T6675	778-786	NN	denotes	estimate
T6676	791-800	VBN	denotes	confirmed
T6677	787-790	VBD	denotes	was
T6678	801-803	IN	denotes	by
T6679	804-810	JJ	denotes	direct
T6680	811-821	NN	denotes	comparison
T6681	822-824	IN	denotes	of
T6682	825-828	NN	denotes	APP
T6683	829-835	NNS	denotes	levels
T6684	836-838	IN	denotes	in
T6685	839-852	JJ	denotes	nontransgenic
T6686	869-873	NNS	denotes	mice
T6687	853-856	CC	denotes	and
T6688	857-860	NN	denotes	tet
T6689	861-864	NN	denotes	off
T6690	860-861	HYPH	denotes	-
T6691	865-868	NN	denotes	APP
T6692	874-879	VBG	denotes	using
T6693	880-882	DT	denotes	an
T6694	883-891	NN	denotes	antibody
T6695	892-896	WDT	denotes	that
T6696	897-907	VBZ	denotes	recognizes
T6697	908-912	CC	denotes	both
T6698	924-927	NN	denotes	APP
T6699	913-923	JJ	denotes	endogenous
T6700	928-929	-LRB-	denotes	(
T6701	929-932	CC	denotes	and
T6702	933-940	NN	denotes	amyloid
T6703	956-963	NN	denotes	protein
T6704	941-950	NN	denotes	precursor
T6705	951-955	JJ	denotes	like
T6706	950-951	HYPH	denotes	-
T6707	964-965	CD	denotes	2
T6708	965-966	-RRB-	denotes	)
T6709	967-970	CC	denotes	and
T6710	971-974	DT	denotes	the
T6711	986-993	NN	denotes	protein
T6712	975-985	JJ	denotes	transgenic
T6713	994-995	-LRB-	denotes	(
T6714	1029-1031	NN	denotes	1D
T6715	995-1005	JJ	denotes	monoclonal
T6716	1015-1020	NN	denotes	22C11
T6717	1006-1014	NN	denotes	antibody
T6718	1020-1021	:	denotes	;
T6719	1022-1028	NN	denotes	Figure
T6720	1031-1032	-RRB-	denotes	)
T6721	1032-1033	.	denotes	.
T6722	1033-2834	sentence	denotes	Figure 1 Control of Transgenic APP Expression by Dox (A) Western blotting for transgenic APP using the human-specific 6E10 antibody shows expression of full-length protein in forebrain tissue from young predeposit double transgenic animals (line 107) and its suppression following dox treatment. Untreated animals show high levels of transgene expression; protein levels drop dramatically in animals acutely treated with dox for 2 wk. A faint, but detectable band of full-length protein remains in acutely treated animals that can be eliminated in mice born and raised on dox. (B) Immunoblotting with the N-terminal antibody 22C11 to detect both transgenic and endogenous protein shows that dox treatment reduces APP/APLP to levels found in nontransgenic mice. (C) Measurement of signal intensity from the Western blot in (A) shows transgenic APP levels are decreased more than 95% by dox in both acutely and chronically treated animals (97.2% for 4 wk + 2 wk dox, 98.0% for reared on dox versus 4 wk untreated; ANOVA effect of treatment group F 4,15 = 85.55, p < 0.001). APP levels in 4 wk + 2 wk dox, reared on dox, and nontransgenic (NTg) were not significantly different (p > 0.9, Tukey post-hoc test). (D) Measurement of signal intensity from the Western blot in (B) shows total APP/APLP levels in dox-treated tTA/APP mice are significantly lower than in 4-wk-old untreated mice (ANOVA effect of treatment group F 4,15 = 84.41, p < 0.001) and indistinguishable from those of nontransgenic animals (p > 0.9, Tukey post-hoc test). *, p < 0.001 versus untreated 4-wk-old mice, Tukey post-hoc test applied to significant effect of group ANOVA. Importantly, all four new lines of tet-off APP mice showed nearly complete suppression of the transgene following dox treatment (Figures 1 and Figure S1).
T6723	2680-2691	RB	denotes	Importantly
T6724	2732-2738	VBD	denotes	showed
T6725	2691-2693	,	denotes	,
T6726	2693-2696	DT	denotes	all
T6727	2706-2711	NNS	denotes	lines
T6728	2697-2701	CD	denotes	four
T6729	2702-2705	JJ	denotes	new
T6730	2712-2714	IN	denotes	of
T6731	2715-2718	NN	denotes	tet
T6732	2719-2722	NN	denotes	off
T6733	2718-2719	HYPH	denotes	-
T6734	2727-2731	NNS	denotes	mice
T6735	2723-2726	NN	denotes	APP
T6736	2739-2745	RB	denotes	nearly
T6737	2746-2754	JJ	denotes	complete
T6738	2755-2766	NN	denotes	suppression
T6739	2767-2769	IN	denotes	of
T6740	2770-2773	DT	denotes	the
T6741	2774-2783	NN	denotes	transgene
T6742	2784-2793	VBG	denotes	following
T6743	2794-2797	NN	denotes	dox
T6744	2798-2807	NN	denotes	treatment
T6745	2808-2809	-LRB-	denotes	(
T6746	2809-2816	NNS	denotes	Figures
T6747	2817-2818	CD	denotes	1
T6748	2819-2822	CC	denotes	and
T6749	2823-2829	NN	denotes	Figure
T6750	2830-2832	NN	denotes	S1
T6751	2832-2833	-RRB-	denotes	)
T6752	2833-2834	.	denotes	.
T6753	2834-3017	sentence	denotes	We focused on one of the four lines, line 107, to examine in more detail the time dependence and extent of transgene suppression following either acute or chronic treatment with dox.
T6754	2835-2837	PRP	denotes	We
T6755	2838-2845	VBD	denotes	focused
T6756	2846-2848	IN	denotes	on
T6757	2849-2852	CD	denotes	one
T6758	2853-2855	IN	denotes	of
T6759	2856-2859	DT	denotes	the
T6760	2865-2870	NNS	denotes	lines
T6761	2860-2864	CD	denotes	four
T6762	2870-2872	,	denotes	,
T6763	2872-2876	NN	denotes	line
T6764	2877-2880	CD	denotes	107
T6765	2880-2882	,	denotes	,
T6766	2882-2884	TO	denotes	to
T6767	2885-2892	VB	denotes	examine
T6768	2893-2895	IN	denotes	in
T6769	2896-2900	JJR	denotes	more
T6770	2901-2907	NN	denotes	detail
T6771	2908-2911	DT	denotes	the
T6772	2917-2927	NN	denotes	dependence
T6773	2912-2916	NN	denotes	time
T6774	2928-2931	CC	denotes	and
T6775	2932-2938	NN	denotes	extent
T6776	2939-2941	IN	denotes	of
T6777	2942-2951	NN	denotes	transgene
T6778	2952-2963	NN	denotes	suppression
T6779	2964-2973	VBG	denotes	following
T6780	2974-2980	CC	denotes	either
T6781	2998-3007	NN	denotes	treatment
T6782	2981-2986	JJ	denotes	acute
T6783	2987-2989	CC	denotes	or
T6784	2990-2997	JJ	denotes	chronic
T6785	3008-3012	IN	denotes	with
T6786	3013-3016	NN	denotes	dox
T6787	3016-3017	.	denotes	.
T6788	3017-3297	sentence	denotes	Two dox-treated groups were compared to two untreated groups: one group of mice was born and raised on dox, a second group was treated with dox for 2 wk starting at 1 mo of age (4 wk + 2 wk dox); two untreated groups kept on normal chow were harvested at either 4 or 6 wk of age.
T6789	3018-3021	CD	denotes	Two
T6790	3034-3040	NNS	denotes	groups
T6791	3022-3025	NN	denotes	dox
T6792	3026-3033	VBN	denotes	treated
T6793	3025-3026	HYPH	denotes	-
T6794	3046-3054	VBN	denotes	compared
T6795	3041-3045	VBD	denotes	were
T6796	3260-3269	VBN	denotes	harvested
T6797	3055-3057	IN	denotes	to
T6798	3058-3061	CD	denotes	two
T6799	3072-3078	NNS	denotes	groups
T6800	3062-3071	JJ	denotes	untreated
T6801	3078-3080	:	denotes	:
T6802	3080-3083	CD	denotes	one
T6803	3084-3089	NN	denotes	group
T6804	3102-3106	VBN	denotes	born
T6805	3090-3092	IN	denotes	of
T6806	3093-3097	NNS	denotes	mice
T6807	3098-3101	VBD	denotes	was
T6808	3107-3110	CC	denotes	and
T6809	3111-3117	VBN	denotes	raised
T6810	3118-3120	IN	denotes	on
T6811	3121-3124	NN	denotes	dox
T6812	3124-3126	,	denotes	,
T6813	3126-3127	DT	denotes	a
T6814	3135-3140	NN	denotes	group
T6815	3128-3134	JJ	denotes	second
T6816	3145-3152	VBN	denotes	treated
T6817	3141-3144	VBD	denotes	was
T6818	3153-3157	IN	denotes	with
T6819	3158-3161	NN	denotes	dox
T6820	3162-3165	IN	denotes	for
T6821	3166-3167	CD	denotes	2
T6822	3168-3170	NN	denotes	wk
T6823	3171-3179	VBG	denotes	starting
T6824	3180-3182	IN	denotes	at
T6825	3183-3184	CD	denotes	1
T6826	3185-3187	NN	denotes	mo
T6827	3188-3190	IN	denotes	of
T6828	3191-3194	NN	denotes	age
T6829	3195-3196	-LRB-	denotes	(
T6830	3198-3200	NN	denotes	wk
T6831	3196-3197	CD	denotes	4
T6832	3201-3202	SYM	denotes	+
T6833	3203-3204	CD	denotes	2
T6834	3205-3207	NN	denotes	wk
T6835	3208-3211	NN	denotes	dox
T6836	3211-3212	-RRB-	denotes	)
T6837	3212-3213	:	denotes	;
T6838	3214-3217	CD	denotes	two
T6839	3228-3234	NNS	denotes	groups
T6840	3218-3227	JJ	denotes	untreated
T6841	3235-3239	VBN	denotes	kept
T6842	3240-3242	IN	denotes	on
T6843	3243-3249	JJ	denotes	normal
T6844	3250-3254	NN	denotes	chow
T6845	3255-3259	VBD	denotes	were
T6846	3270-3272	IN	denotes	at
T6847	3273-3279	CC	denotes	either
T6848	3280-3281	CD	denotes	4
T6849	3287-3289	NN	denotes	wk
T6850	3282-3284	CC	denotes	or
T6851	3285-3286	CD	denotes	6
T6852	3290-3292	IN	denotes	of
T6853	3293-3296	NN	denotes	age
T6854	3296-3297	.	denotes	.
T6855	3297-3368	sentence	denotes	Animals born and raised on dox harbored no transgenic APP (Figure 1A).
T6856	3298-3305	NNS	denotes	Animals
T6857	3329-3337	VBD	denotes	harbored
T6858	3306-3310	VBN	denotes	born
T6859	3311-3314	CC	denotes	and
T6860	3315-3321	VBN	denotes	raised
T6861	3322-3324	IN	denotes	on
T6862	3325-3328	NN	denotes	dox
T6863	3338-3340	DT	denotes	no
T6864	3352-3355	NN	denotes	APP
T6865	3341-3351	JJ	denotes	transgenic
T6866	3356-3357	-LRB-	denotes	(
T6867	3364-3366	NN	denotes	1A
T6868	3357-3363	NN	denotes	Figure
T6869	3366-3367	-RRB-	denotes	)
T6870	3367-3368	.	denotes	.
T6871	3368-3497	sentence	denotes	Following as little as 2 wk of dox treatment, transgenic APP expression was reduced by more than 95% compared to pre-dox levels.
T6872	3369-3378	VBG	denotes	Following
T6873	3445-3452	VBN	denotes	reduced
T6874	3379-3381	RB	denotes	as
T6875	3392-3393	CD	denotes	2
T6876	3382-3388	JJ	denotes	little
T6877	3389-3391	IN	denotes	as
T6878	3394-3396	NN	denotes	wk
T6879	3397-3399	IN	denotes	of
T6880	3400-3403	NN	denotes	dox
T6881	3404-3413	NN	denotes	treatment
T6882	3413-3415	,	denotes	,
T6883	3415-3425	JJ	denotes	transgenic
T6884	3430-3440	NN	denotes	expression
T6885	3426-3429	NN	denotes	APP
T6886	3441-3444	VBD	denotes	was
T6887	3453-3455	IN	denotes	by
T6888	3456-3460	JJR	denotes	more
T6889	3466-3468	CD	denotes	95
T6890	3461-3465	IN	denotes	than
T6891	3468-3469	NN	denotes	%
T6892	3470-3478	VBN	denotes	compared
T6893	3479-3481	IN	denotes	to
T6894	3482-3489	JJ	denotes	pre-dox
T6895	3490-3496	NNS	denotes	levels
T6896	3496-3497	.	denotes	.
T6897	3497-3736	sentence	denotes	The residual expression remaining in acutely treated mice represents less than 4% of the transgenic protein produced in the absence of dox (Figure 1C), and likely results from slight leakage at the level of transcription (data not shown).
T6898	3498-3501	DT	denotes	The
T6899	3511-3521	NN	denotes	expression
T6900	3502-3510	JJ	denotes	residual
T6901	3556-3566	VBZ	denotes	represents
T6902	3522-3531	VBG	denotes	remaining
T6903	3532-3534	IN	denotes	in
T6904	3535-3542	RB	denotes	acutely
T6905	3543-3550	VBN	denotes	treated
T6906	3551-3555	NNS	denotes	mice
T6907	3567-3571	JJR	denotes	less
T6908	3577-3578	CD	denotes	4
T6909	3572-3576	IN	denotes	than
T6910	3578-3579	NN	denotes	%
T6911	3580-3582	IN	denotes	of
T6912	3583-3586	DT	denotes	the
T6913	3598-3605	NN	denotes	protein
T6914	3587-3597	JJ	denotes	transgenic
T6915	3606-3614	VBN	denotes	produced
T6916	3615-3617	IN	denotes	in
T6917	3618-3621	DT	denotes	the
T6918	3622-3629	NN	denotes	absence
T6919	3630-3632	IN	denotes	of
T6920	3633-3636	NN	denotes	dox
T6921	3637-3638	-LRB-	denotes	(
T6922	3645-3647	NN	denotes	1C
T6923	3638-3644	NN	denotes	Figure
T6924	3647-3648	-RRB-	denotes	)
T6925	3648-3650	,	denotes	,
T6926	3650-3653	CC	denotes	and
T6927	3654-3660	RB	denotes	likely
T6928	3661-3668	VBZ	denotes	results
T6929	3669-3673	IN	denotes	from
T6930	3674-3680	JJ	denotes	slight
T6931	3681-3688	NN	denotes	leakage
T6932	3689-3691	IN	denotes	at
T6933	3692-3695	DT	denotes	the
T6934	3696-3701	NN	denotes	level
T6935	3702-3704	IN	denotes	of
T6936	3705-3718	NN	denotes	transcription
T6937	3719-3720	-LRB-	denotes	(
T6938	3729-3734	VBN	denotes	shown
T6939	3720-3724	NNS	denotes	data
T6940	3725-3728	RB	denotes	not
T6941	3734-3735	-RRB-	denotes	)
T6942	3735-3736	.	denotes	.
T6943	3736-4055	sentence	denotes	Importantly, the total amount of APP (endogenous plus transgenic) and related APLPs in both acute and chronically treated animals was statistically indistinguishable from that in nontransgenic mice (Figure 1D; statistical analyses for experiments throughout the study are presented in the accompanying figure legends).
T6944	3737-3748	RB	denotes	Importantly
T6945	3867-3870	VBD	denotes	was
T6946	3748-3750	,	denotes	,
T6947	3750-3753	DT	denotes	the
T6948	3760-3766	NN	denotes	amount
T6949	3754-3759	JJ	denotes	total
T6950	3767-3769	IN	denotes	of
T6951	3770-3773	NN	denotes	APP
T6952	3774-3775	-LRB-	denotes	(
T6953	3775-3785	JJ	denotes	endogenous
T6954	3786-3790	CC	denotes	plus
T6955	3791-3801	JJ	denotes	transgenic
T6956	3801-3802	-RRB-	denotes	)
T6957	3803-3806	CC	denotes	and
T6958	3807-3814	JJ	denotes	related
T6959	3815-3820	NNS	denotes	APLPs
T6960	3821-3823	IN	denotes	in
T6961	3824-3828	CC	denotes	both
T6962	3829-3834	JJ	denotes	acute
T6963	3859-3866	NNS	denotes	animals
T6964	3835-3838	CC	denotes	and
T6965	3839-3850	RB	denotes	chronically
T6966	3851-3858	VBN	denotes	treated
T6967	3871-3884	RB	denotes	statistically
T6968	3885-3902	JJ	denotes	indistinguishable
T6969	3903-3907	IN	denotes	from
T6970	3908-3912	DT	denotes	that
T6971	3913-3915	IN	denotes	in
T6972	3916-3929	JJ	denotes	nontransgenic
T6973	3930-3934	NNS	denotes	mice
T6974	3935-3936	-LRB-	denotes	(
T6975	3943-3945	NN	denotes	1D
T6976	3936-3942	NN	denotes	Figure
T6977	3945-3946	:	denotes	;
T6978	3947-3958	JJ	denotes	statistical
T6979	3959-3967	NNS	denotes	analyses
T6980	4009-4018	VBN	denotes	presented
T6981	3968-3971	IN	denotes	for
T6982	3972-3983	NNS	denotes	experiments
T6983	3984-3994	IN	denotes	throughout
T6984	3995-3998	DT	denotes	the
T6985	3999-4004	NN	denotes	study
T6986	4005-4008	VBP	denotes	are
T6987	4019-4021	IN	denotes	in
T6988	4022-4025	DT	denotes	the
T6989	4046-4053	NNS	denotes	legends
T6990	4026-4038	VBG	denotes	accompanying
T6991	4039-4045	NN	denotes	figure
T6992	4053-4054	-RRB-	denotes	)
T6993	4054-4055	.	denotes	.
T6994	4055-4267	sentence	denotes	To ensure that Aβ production was suppressed in concert with the dox-mediated inhibition of its precursor APPswe/ind, we measured Aβ40 and Aβ42 levels by ELISA in forebrain homogenates from young tet-off animals.
T6995	4056-4058	TO	denotes	To
T6996	4059-4065	VB	denotes	ensure
T6997	4176-4184	VBD	denotes	measured
T6998	4066-4070	IN	denotes	that
T6999	4089-4099	VBN	denotes	suppressed
T7000	4071-4073	NN	denotes	Aβ
T7001	4074-4084	NN	denotes	production
T7002	4085-4088	VBD	denotes	was
T7003	4100-4102	IN	denotes	in
T7004	4103-4110	NN	denotes	concert
T7005	4111-4115	IN	denotes	with
T7006	4116-4119	DT	denotes	the
T7007	4133-4143	NN	denotes	inhibition
T7008	4120-4123	NN	denotes	dox
T7009	4124-4132	VBN	denotes	mediated
T7010	4123-4124	HYPH	denotes	-
T7011	4144-4146	IN	denotes	of
T7012	4147-4150	PRP$	denotes	its
T7013	4151-4160	NN	denotes	precursor
T7014	4161-4167	NN	denotes	APPswe
T7015	4168-4171	NN	denotes	ind
T7016	4167-4168	HYPH	denotes	/
T7017	4171-4173	,	denotes	,
T7018	4173-4175	PRP	denotes	we
T7019	4185-4189	NN	denotes	Aβ40
T7020	4199-4205	NNS	denotes	levels
T7021	4190-4193	CC	denotes	and
T7022	4194-4198	NN	denotes	Aβ42
T7023	4206-4208	IN	denotes	by
T7024	4209-4214	NN	denotes	ELISA
T7025	4215-4217	IN	denotes	in
T7026	4218-4227	NN	denotes	forebrain
T7027	4228-4239	NNS	denotes	homogenates
T7028	4240-4244	IN	denotes	from
T7029	4245-4250	JJ	denotes	young
T7030	4259-4266	NNS	denotes	animals
T7031	4251-4254	NN	denotes	tet
T7032	4255-4258	NN	denotes	off
T7033	4254-4255	HYPH	denotes	-
T7034	4266-4267	.	denotes	.
T7035	4267-4444	sentence	denotes	At 1 mo of age, the mice lacked visible amyloid aggregates that might act as an intractable reservoir of peptide remaining in the brain after the transgene had been suppressed.
T7036	4268-4270	IN	denotes	At
T7037	4293-4299	VBD	denotes	lacked
T7038	4271-4272	CD	denotes	1
T7039	4273-4275	NN	denotes	mo
T7040	4276-4278	IN	denotes	of
T7041	4279-4282	NN	denotes	age
T7042	4282-4284	,	denotes	,
T7043	4284-4287	DT	denotes	the
T7044	4288-4292	NNS	denotes	mice
T7045	4300-4307	JJ	denotes	visible
T7046	4316-4326	NNS	denotes	aggregates
T7047	4308-4315	NN	denotes	amyloid
T7048	4327-4331	WDT	denotes	that
T7049	4338-4341	VB	denotes	act
T7050	4332-4337	MD	denotes	might
T7051	4342-4344	IN	denotes	as
T7052	4345-4347	DT	denotes	an
T7053	4360-4369	NN	denotes	reservoir
T7054	4348-4359	JJ	denotes	intractable
T7055	4370-4372	IN	denotes	of
T7056	4373-4380	NN	denotes	peptide
T7057	4381-4390	VBG	denotes	remaining
T7058	4391-4393	IN	denotes	in
T7059	4394-4397	DT	denotes	the
T7060	4398-4403	NN	denotes	brain
T7061	4404-4409	IN	denotes	after
T7062	4433-4443	VBN	denotes	suppressed
T7063	4410-4413	DT	denotes	the
T7064	4414-4423	NN	denotes	transgene
T7065	4424-4427	VBD	denotes	had
T7066	4428-4432	VBN	denotes	been
T7067	4443-4444	.	denotes	.
T7068	4444-4690	sentence	denotes	To further ensure we could detect any such insoluble aggregates that might bias our measure of changes in peptide synthesis, we performed a sequential three-step extraction with PBS, 2% SDS, and 70% FA that would separate peptides by solubility.
T7069	4445-4447	TO	denotes	To
T7070	4456-4462	VB	denotes	ensure
T7071	4448-4455	RB	denotes	further
T7072	4573-4582	VBD	denotes	performed
T7073	4463-4465	PRP	denotes	we
T7074	4472-4478	VB	denotes	detect
T7075	4466-4471	MD	denotes	could
T7076	4479-4482	DT	denotes	any
T7077	4498-4508	NNS	denotes	aggregates
T7078	4483-4487	JJ	denotes	such
T7079	4488-4497	JJ	denotes	insoluble
T7080	4509-4513	WDT	denotes	that
T7081	4520-4524	VB	denotes	bias
T7082	4514-4519	MD	denotes	might
T7083	4525-4528	PRP$	denotes	our
T7084	4529-4536	NN	denotes	measure
T7085	4537-4539	IN	denotes	of
T7086	4540-4547	NNS	denotes	changes
T7087	4548-4550	IN	denotes	in
T7088	4551-4558	NN	denotes	peptide
T7089	4559-4568	NN	denotes	synthesis
T7090	4568-4570	,	denotes	,
T7091	4570-4572	PRP	denotes	we
T7092	4583-4584	DT	denotes	a
T7093	4607-4617	NN	denotes	extraction
T7094	4585-4595	JJ	denotes	sequential
T7095	4596-4601	CD	denotes	three
T7096	4602-4606	NN	denotes	step
T7097	4601-4602	HYPH	denotes	-
T7098	4618-4622	IN	denotes	with
T7099	4623-4626	NN	denotes	PBS
T7100	4626-4628	,	denotes	,
T7101	4628-4629	CD	denotes	2
T7102	4629-4630	NN	denotes	%
T7103	4631-4634	NN	denotes	SDS
T7104	4634-4636	,	denotes	,
T7105	4636-4639	CC	denotes	and
T7106	4640-4642	CD	denotes	70
T7107	4642-4643	NN	denotes	%
T7108	4644-4646	NN	denotes	FA
T7109	4647-4651	WDT	denotes	that
T7110	4658-4666	VB	denotes	separate
T7111	4652-4657	MD	denotes	would
T7112	4667-4675	NNS	denotes	peptides
T7113	4676-4678	IN	denotes	by
T7114	4679-4689	NN	denotes	solubility
T7115	4689-4690	.	denotes	.
T7116	4690-5033	sentence	denotes	We compared the levels of human transgene-derived Aβ40 and Aβ42 in untreated mice at 4 and 6 wk of age to animals that had either been born and raised on dox or that had been left untreated for 4 wk and then placed on dox chow for 2 wk prior to harvest (the same groups described above for immunoblot analysis of APPswe/ind levels, line 107).
T7117	4691-4693	PRP	denotes	We
T7118	4694-4702	VBD	denotes	compared
T7119	4703-4706	DT	denotes	the
T7120	4707-4713	NNS	denotes	levels
T7121	4714-4716	IN	denotes	of
T7122	4717-4722	JJ	denotes	human
T7123	4723-4732	NN	denotes	transgene
T7124	4733-4740	VBN	denotes	derived
T7125	4732-4733	HYPH	denotes	-
T7126	4741-4745	NN	denotes	Aβ40
T7127	4746-4749	CC	denotes	and
T7128	4750-4754	NN	denotes	Aβ42
T7129	4755-4757	IN	denotes	in
T7130	4758-4767	JJ	denotes	untreated
T7131	4768-4772	NNS	denotes	mice
T7132	4773-4775	IN	denotes	at
T7133	4776-4777	CD	denotes	4
T7134	4784-4786	NN	denotes	wk
T7135	4778-4781	CC	denotes	and
T7136	4782-4783	CD	denotes	6
T7137	4787-4789	IN	denotes	of
T7138	4790-4793	NN	denotes	age
T7139	4794-4796	IN	denotes	to
T7140	4797-4804	NNS	denotes	animals
T7141	4805-4809	WDT	denotes	that
T7142	4826-4830	VBN	denotes	born
T7143	4810-4813	VBD	denotes	had
T7144	4814-4820	CC	denotes	either
T7145	4821-4825	VBN	denotes	been
T7146	4831-4834	CC	denotes	and
T7147	4835-4841	VBN	denotes	raised
T7148	4842-4844	IN	denotes	on
T7149	4845-4848	NN	denotes	dox
T7150	4849-4851	CC	denotes	or
T7151	4852-4856	WDT	denotes	that
T7152	4866-4870	VBN	denotes	left
T7153	4857-4860	VBD	denotes	had
T7154	4861-4865	VBN	denotes	been
T7155	4871-4880	JJ	denotes	untreated
T7156	4881-4884	IN	denotes	for
T7157	4885-4886	CD	denotes	4
T7158	4887-4889	NN	denotes	wk
T7159	4890-4893	CC	denotes	and
T7160	4894-4898	RB	denotes	then
T7161	4899-4905	VBN	denotes	placed
T7162	4906-4908	IN	denotes	on
T7163	4909-4912	NN	denotes	dox
T7164	4913-4917	NN	denotes	chow
T7165	4918-4921	IN	denotes	for
T7166	4922-4923	CD	denotes	2
T7167	4924-4926	NN	denotes	wk
T7168	4927-4932	JJ	denotes	prior
T7169	4933-4935	IN	denotes	to
T7170	4936-4943	NN	denotes	harvest
T7171	4944-4945	-LRB-	denotes	(
T7172	5023-5027	NN	denotes	line
T7173	4945-4948	DT	denotes	the
T7174	4954-4960	NNS	denotes	groups
T7175	4949-4953	JJ	denotes	same
T7176	4961-4970	VBN	denotes	described
T7177	4971-4976	RB	denotes	above
T7178	4977-4980	IN	denotes	for
T7179	4981-4991	NN	denotes	immunoblot
T7180	4992-5000	NN	denotes	analysis
T7181	5001-5003	IN	denotes	of
T7182	5004-5010	NN	denotes	APPswe
T7183	5011-5014	NN	denotes	ind
T7184	5010-5011	HYPH	denotes	/
T7185	5015-5021	NNS	denotes	levels
T7186	5021-5023	,	denotes	,
T7187	5028-5031	CD	denotes	107
T7188	5031-5032	-RRB-	denotes	)
T7189	5032-5033	.	denotes	.
T7190	5033-5309	sentence	denotes	Consistent with the reduction in full-length APPswe/ind synthesis shown by immunoblot (see Figure 1), we found that transgene-derived Aβ levels were completely suppressed in animals born and raised on dox, and were sharply reduced following acute (2 wk) antibiotic treatment.
T7191	5034-5044	JJ	denotes	Consistent
T7192	5139-5144	VBD	denotes	found
T7193	5045-5049	IN	denotes	with
T7194	5050-5053	DT	denotes	the
T7195	5054-5063	NN	denotes	reduction
T7196	5064-5066	IN	denotes	in
T7197	5067-5071	JJ	denotes	full
T7198	5072-5078	NN	denotes	length
T7199	5071-5072	HYPH	denotes	-
T7200	5090-5099	NN	denotes	synthesis
T7201	5079-5085	NN	denotes	APPswe
T7202	5086-5089	NN	denotes	ind
T7203	5085-5086	HYPH	denotes	/
T7204	5100-5105	VBN	denotes	shown
T7205	5106-5108	IN	denotes	by
T7206	5109-5119	NN	denotes	immunoblot
T7207	5120-5121	-LRB-	denotes	(
T7208	5121-5124	VB	denotes	see
T7209	5125-5131	NN	denotes	Figure
T7210	5132-5133	CD	denotes	1
T7211	5133-5134	-RRB-	denotes	)
T7212	5134-5136	,	denotes	,
T7213	5136-5138	PRP	denotes	we
T7214	5145-5149	IN	denotes	that
T7215	5194-5204	VBN	denotes	suppressed
T7216	5150-5159	NN	denotes	transgene
T7217	5160-5167	VBN	denotes	derived
T7218	5159-5160	HYPH	denotes	-
T7219	5171-5177	NNS	denotes	levels
T7220	5168-5170	NN	denotes	Aβ
T7221	5178-5182	VBD	denotes	were
T7222	5183-5193	RB	denotes	completely
T7223	5205-5207	IN	denotes	in
T7224	5208-5215	NNS	denotes	animals
T7225	5216-5220	VBN	denotes	born
T7226	5221-5224	CC	denotes	and
T7227	5225-5231	VBN	denotes	raised
T7228	5232-5234	IN	denotes	on
T7229	5235-5238	NN	denotes	dox
T7230	5238-5240	,	denotes	,
T7231	5240-5243	CC	denotes	and
T7232	5244-5248	VBD	denotes	were
T7233	5257-5264	VBN	denotes	reduced
T7234	5249-5256	RB	denotes	sharply
T7235	5265-5274	VBG	denotes	following
T7236	5275-5280	JJ	denotes	acute
T7237	5299-5308	NN	denotes	treatment
T7238	5281-5282	-LRB-	denotes	(
T7239	5284-5286	NN	denotes	wk
T7240	5282-5283	CD	denotes	2
T7241	5286-5287	-RRB-	denotes	)
T7242	5288-5298	JJ	denotes	antibiotic
T7243	5308-5309	.	denotes	.
T7244	5309-5475	sentence	denotes	Compared to the levels in untreated 4-wk-old mice, PBS-soluble Aβ42 dropped by 95.2% following 2 wk of dox treatment and by 99.2% with chronic treatment (Figure 2A).
T7245	5310-5318	VBN	denotes	Compared
T7246	5378-5385	VBD	denotes	dropped
T7247	5319-5321	IN	denotes	to
T7248	5322-5325	DT	denotes	the
T7249	5326-5332	NNS	denotes	levels
T7250	5333-5335	IN	denotes	in
T7251	5336-5345	JJ	denotes	untreated
T7252	5355-5359	NNS	denotes	mice
T7253	5346-5347	CD	denotes	4
T7254	5348-5350	NN	denotes	wk
T7255	5347-5348	HYPH	denotes	-
T7256	5351-5354	JJ	denotes	old
T7257	5350-5351	HYPH	denotes	-
T7258	5359-5361	,	denotes	,
T7259	5361-5364	NN	denotes	PBS
T7260	5365-5372	JJ	denotes	soluble
T7261	5364-5365	HYPH	denotes	-
T7262	5373-5377	NN	denotes	Aβ42
T7263	5386-5388	IN	denotes	by
T7264	5389-5393	CD	denotes	95.2
T7265	5393-5394	NN	denotes	%
T7266	5395-5404	VBG	denotes	following
T7267	5405-5406	CD	denotes	2
T7268	5407-5409	NN	denotes	wk
T7269	5410-5412	IN	denotes	of
T7270	5413-5416	NN	denotes	dox
T7271	5417-5426	NN	denotes	treatment
T7272	5427-5430	CC	denotes	and
T7273	5431-5433	IN	denotes	by
T7274	5440-5444	IN	denotes	with
T7275	5434-5438	CD	denotes	99.2
T7276	5438-5439	NN	denotes	%
T7277	5445-5452	JJ	denotes	chronic
T7278	5453-5462	NN	denotes	treatment
T7279	5463-5464	-LRB-	denotes	(
T7280	5471-5473	NN	denotes	2A
T7281	5464-5470	NN	denotes	Figure
T7282	5473-5474	-RRB-	denotes	)
T7283	5474-5475	.	denotes	.
T7284	5475-5586	sentence	denotes	Similarly, SDS-soluble Aβ42 decreased by 75.2% and 94.8% following 2-wk or lifelong dox treatment (Figure 2B).
T7285	5476-5485	RB	denotes	Similarly
T7286	5504-5513	VBD	denotes	decreased
T7287	5485-5487	,	denotes	,
T7288	5487-5490	NN	denotes	SDS
T7289	5491-5498	JJ	denotes	soluble
T7290	5490-5491	HYPH	denotes	-
T7291	5499-5503	NN	denotes	Aβ42
T7292	5514-5516	IN	denotes	by
T7293	5517-5521	CD	denotes	75.2
T7294	5521-5522	NN	denotes	%
T7295	5523-5526	CC	denotes	and
T7296	5527-5531	CD	denotes	94.8
T7297	5531-5532	NN	denotes	%
T7298	5533-5542	VBG	denotes	following
T7299	5543-5544	CD	denotes	2
T7300	5545-5547	NN	denotes	wk
T7301	5544-5545	HYPH	denotes	-
T7302	5564-5573	NN	denotes	treatment
T7303	5548-5550	CC	denotes	or
T7304	5551-5559	JJ	denotes	lifelong
T7305	5560-5563	NN	denotes	dox
T7306	5574-5575	-LRB-	denotes	(
T7307	5582-5584	NN	denotes	2B
T7308	5575-5581	NN	denotes	Figure
T7309	5584-5585	-RRB-	denotes	)
T7310	5585-5586	.	denotes	.
T7311	5586-5820	sentence	denotes	Only the FA fraction revealed a small dox-resistant pool of peptide in acutely treated animals that we believe represents stable predeposit aggregates that have already accumulated by 4 wk of age when treatment was begun (Figure 2C).
T7312	5587-5591	RB	denotes	Only
T7313	5599-5607	NN	denotes	fraction
T7314	5592-5595	DT	denotes	the
T7315	5596-5598	NN	denotes	FA
T7316	5608-5616	VBD	denotes	revealed
T7317	5617-5618	DT	denotes	a
T7318	5639-5643	NN	denotes	pool
T7319	5619-5624	JJ	denotes	small
T7320	5625-5628	NN	denotes	dox
T7321	5629-5638	JJ	denotes	resistant
T7322	5628-5629	HYPH	denotes	-
T7323	5644-5646	IN	denotes	of
T7324	5647-5654	NN	denotes	peptide
T7325	5655-5657	IN	denotes	in
T7326	5658-5665	RB	denotes	acutely
T7327	5666-5673	VBN	denotes	treated
T7328	5674-5681	NNS	denotes	animals
T7329	5682-5686	WDT	denotes	that
T7330	5690-5697	VBP	denotes	believe
T7331	5687-5689	PRP	denotes	we
T7332	5698-5708	VBZ	denotes	represents
T7333	5709-5715	JJ	denotes	stable
T7334	5727-5737	NNS	denotes	aggregates
T7335	5716-5726	JJ	denotes	predeposit
T7336	5738-5742	WDT	denotes	that
T7337	5756-5767	VBN	denotes	accumulated
T7338	5743-5747	VBP	denotes	have
T7339	5748-5755	RB	denotes	already
T7340	5768-5770	IN	denotes	by
T7341	5771-5772	CD	denotes	4
T7342	5773-5775	NN	denotes	wk
T7343	5776-5778	IN	denotes	of
T7344	5779-5782	NN	denotes	age
T7345	5783-5787	WRB	denotes	when
T7346	5802-5807	VBN	denotes	begun
T7347	5788-5797	NN	denotes	treatment
T7348	5798-5801	VBD	denotes	was
T7349	5808-5809	-LRB-	denotes	(
T7350	5816-5818	NN	denotes	2C
T7351	5809-5815	NN	denotes	Figure
T7352	5818-5819	-RRB-	denotes	)
T7353	5819-5820	.	denotes	.
T7354	5820-5982	sentence	denotes	Indeed, animals that were born and raised on dox did not harbor this reservoir of treatment-resistant peptide, with 96.3% less Aβ42 than untreated 4-wk-old mice.
T7355	5821-5827	RB	denotes	Indeed
T7356	5878-5884	VB	denotes	harbor
T7357	5827-5829	,	denotes	,
T7358	5829-5836	NNS	denotes	animals
T7359	5837-5841	WDT	denotes	that
T7360	5847-5851	VBN	denotes	born
T7361	5842-5846	VBD	denotes	were
T7362	5852-5855	CC	denotes	and
T7363	5856-5862	VBN	denotes	raised
T7364	5863-5865	IN	denotes	on
T7365	5866-5869	NN	denotes	dox
T7366	5870-5873	VBD	denotes	did
T7367	5874-5877	RB	denotes	not
T7368	5885-5889	DT	denotes	this
T7369	5890-5899	NN	denotes	reservoir
T7370	5900-5902	IN	denotes	of
T7371	5903-5912	NN	denotes	treatment
T7372	5913-5922	JJ	denotes	resistant
T7373	5912-5913	HYPH	denotes	-
T7374	5923-5930	NN	denotes	peptide
T7375	5930-5932	,	denotes	,
T7376	5932-5936	IN	denotes	with
T7377	5937-5941	CD	denotes	96.3
T7378	5941-5942	NN	denotes	%
T7379	5943-5947	JJR	denotes	less
T7380	5948-5952	NN	denotes	Aβ42
T7381	5953-5957	IN	denotes	than
T7382	5958-5967	JJ	denotes	untreated
T7383	5977-5981	NNS	denotes	mice
T7384	5968-5969	CD	denotes	4
T7385	5970-5972	NN	denotes	wk
T7386	5969-5970	HYPH	denotes	-
T7387	5973-5976	JJ	denotes	old
T7388	5972-5973	HYPH	denotes	-
T7389	5981-5982	.	denotes	.
T7390	5982-6227	sentence	denotes	Measurement of total Aβ in chronically treated mice, including endogenous and transgene-derived peptide, demonstrated that Aβ levels in tet-off APP mice were reduced to the level of endogenous peptide found in nontransgenic animals (Figure 2D).
T7391	5983-5994	NN	denotes	Measurement
T7392	6088-6100	VBD	denotes	demonstrated
T7393	5995-5997	IN	denotes	of
T7394	5998-6003	JJ	denotes	total
T7395	6004-6006	NN	denotes	Aβ
T7396	6007-6009	IN	denotes	in
T7397	6010-6021	RB	denotes	chronically
T7398	6022-6029	VBN	denotes	treated
T7399	6030-6034	NNS	denotes	mice
T7400	6034-6036	,	denotes	,
T7401	6036-6045	VBG	denotes	including
T7402	6046-6056	JJ	denotes	endogenous
T7403	6079-6086	NN	denotes	peptide
T7404	6057-6060	CC	denotes	and
T7405	6061-6070	NN	denotes	transgene
T7406	6071-6078	VBN	denotes	derived
T7407	6070-6071	HYPH	denotes	-
T7408	6086-6088	,	denotes	,
T7409	6101-6105	IN	denotes	that
T7410	6141-6148	VBN	denotes	reduced
T7411	6106-6108	NN	denotes	Aβ
T7412	6109-6115	NNS	denotes	levels
T7413	6116-6118	IN	denotes	in
T7414	6119-6122	NN	denotes	tet
T7415	6123-6126	NN	denotes	off
T7416	6122-6123	HYPH	denotes	-
T7417	6131-6135	NNS	denotes	mice
T7418	6127-6130	NN	denotes	APP
T7419	6136-6140	VBD	denotes	were
T7420	6149-6151	IN	denotes	to
T7421	6152-6155	DT	denotes	the
T7422	6156-6161	NN	denotes	level
T7423	6162-6164	IN	denotes	of
T7424	6165-6175	JJ	denotes	endogenous
T7425	6176-6183	NN	denotes	peptide
T7426	6184-6189	VBN	denotes	found
T7427	6190-6192	IN	denotes	in
T7428	6193-6206	JJ	denotes	nontransgenic
T7429	6207-6214	NNS	denotes	animals
T7430	6215-6216	-LRB-	denotes	(
T7431	6223-6225	NN	denotes	2D
T7432	6216-6222	NN	denotes	Figure
T7433	6225-6226	-RRB-	denotes	)
T7434	6226-6227	.	denotes	.
T7435	6227-6438	sentence	denotes	Taken together with the immunoblotting data for full-length APPswe/ind, the ELISA measurements indicate that dox-mediated suppression of transgenic APPswe/ind synthesis leads to parallel reduction of Aβ levels.
T7436	6228-6233	VBN	denotes	Taken
T7437	6323-6331	VBP	denotes	indicate
T7438	6234-6242	RB	denotes	together
T7439	6243-6247	IN	denotes	with
T7440	6248-6251	DT	denotes	the
T7441	6267-6271	NNS	denotes	data
T7442	6252-6266	NN	denotes	immunoblotting
T7443	6272-6275	IN	denotes	for
T7444	6276-6280	JJ	denotes	full
T7445	6281-6287	NN	denotes	length
T7446	6280-6281	HYPH	denotes	-
T7447	6295-6298	NN	denotes	ind
T7448	6288-6294	NN	denotes	APPswe
T7449	6294-6295	HYPH	denotes	/
T7450	6298-6300	,	denotes	,
T7451	6300-6303	DT	denotes	the
T7452	6310-6322	NNS	denotes	measurements
T7453	6304-6309	NN	denotes	ELISA
T7454	6332-6336	IN	denotes	that
T7455	6397-6402	VBZ	denotes	leads
T7456	6337-6340	NN	denotes	dox
T7457	6341-6349	VBN	denotes	mediated
T7458	6340-6341	HYPH	denotes	-
T7459	6350-6361	NN	denotes	suppression
T7460	6362-6364	IN	denotes	of
T7461	6365-6375	JJ	denotes	transgenic
T7462	6387-6396	NN	denotes	synthesis
T7463	6376-6382	NN	denotes	APPswe
T7464	6383-6386	NN	denotes	ind
T7465	6382-6383	HYPH	denotes	/
T7466	6403-6405	IN	denotes	to
T7467	6406-6414	JJ	denotes	parallel
T7468	6415-6424	NN	denotes	reduction
T7469	6425-6427	IN	denotes	of
T7470	6428-6430	NN	denotes	Aβ
T7471	6431-6437	NNS	denotes	levels
T7472	6437-6438	.	denotes	.
T7473	6438-9425	sentence	denotes	Figure 2 Aβ Levels Are Dramatically Reduced by Transgene Suppression Cortical homogenates from young, predeposit tTA/APP mice used for Western blot in Figure 1 (line 107) were fractionated by sequential multi-step extraction with PBS, 2% SDS, and 70% FA followed by human-specific Aβ ELISA to measure transgene-derived peptide in each fraction. Aβ40 is shown in white, Aβ42 in black. (A) PBS-soluble Aβ levels are substantially reduced by both acute and chronic dox treatment (ANOVA, effect of treatment group F 4,24 = 137.10 and 386.01, p < 0.001, for Aβ40 and Aβ42, respectively). Aβ levels in treated animals are indistinguishable from nontransgenic (NTg) animals (p > 0.3, Tukey post-hoc test). (B) In the young animals tested here prior to the formation of visible amyloid deposits, most Aβ is extracted into the SDS fraction (84% and 76% of all transgene-derived Aβ40 and Aβ42, respectively). As in the PBS-soluble fraction, Aβ levels in the SDS fraction are significantly lowered by dox treatment compared to untreated animals (ANOVA effect of group F 4,24 = 197.57 and 163.48, p < 0.001, for Aβ40 and Aβ42, respectively). Acutely treated animals retained a small (although significant) amount of residual peptide (p < 0.001 compared to nontransgeinc, Tukey post-hoc test), whereas Aβ levels in mice born and raised on dox were reduced to levels indistinguishable from nontransgenic (p > 0.8, Tukey post-hoc test). (C) The FA-soluble fraction already contains a small but significant pool of aggregated Aβ42 in untreated animals by 4 wk of age (p < 0.05 versus nontransgenic; Tukey post-hoc test applied to significant effect of group ANOVA F 4,24 = 17.11, p < 0.001). By 6 wk of age, the amount of Aβ in the FA fraction is increased significantly preceding the appearance of visible deposits 2 wk later. The FA pool is the only peptide fraction not lowered by acute dox treatment (4 wk untreated = 4 wk + 2 wk dox, p > 0.9, Tukey post-hoc test), consistent with poor turnover of aggregated Aβ species. (D) Measurements of total Aβ, including both endogenous and transgene-derived peptides, show that animals born and raised on dox harbor Aβ levels identical to nontransgenic animals (p > 0.9, Tukey post-hoc test, effect of group ANOVA F 4,24 = 39.13 and 35.29, p < 0.001, for Aβ40 and Aβ42, respectively). Whereas chronic transgene suppression fully prevents synthesis of both peptides, acute dox treatment fully suppresses Aβ40 levels (p > 0.8, Tukey post-hoc test), but leaves a small amount of nonsuppressed Aβ42. The residual Aβ42 observed in acutely treated young animals derives from uncleared aggregates extracted in the SDS and FA fractions. , p < 0.05; , p < 0.005; **, p < 0.001 versus 4-wk-old untreated mice, Tukey post-hoc applied to significant effect of group ANOVA. The ELISA data also confirmed that incorporation of the Swedish and Indiana mutations led to high levels of Aβ42, which we predicted would induce rapid plaque formation in untreated animals.
T7474	9235-9238	DT	denotes	The
T7475	9245-9249	NNS	denotes	data
T7476	9239-9244	NN	denotes	ELISA
T7477	9255-9264	VBD	denotes	confirmed
T7478	9250-9254	RB	denotes	also
T7479	9265-9269	IN	denotes	that
T7480	9321-9324	VBD	denotes	led
T7481	9270-9283	NN	denotes	incorporation
T7482	9284-9286	IN	denotes	of
T7483	9287-9290	DT	denotes	the
T7484	9311-9320	NNS	denotes	mutations
T7485	9291-9298	JJ	denotes	Swedish
T7486	9299-9302	CC	denotes	and
T7487	9303-9310	NNP	denotes	Indiana
T7488	9325-9327	IN	denotes	to
T7489	9328-9332	JJ	denotes	high
T7490	9333-9339	NNS	denotes	levels
T7491	9340-9342	IN	denotes	of
T7492	9343-9347	NN	denotes	Aβ42
T7493	9347-9349	,	denotes	,
T7494	9349-9354	WDT	denotes	which
T7495	9358-9367	VBD	denotes	predicted
T7496	9355-9357	PRP	denotes	we
T7497	9368-9373	MD	denotes	would
T7498	9374-9380	VB	denotes	induce
T7499	9381-9386	JJ	denotes	rapid
T7500	9394-9403	NN	denotes	formation
T7501	9387-9393	NN	denotes	plaque
T7502	9404-9406	IN	denotes	in
T7503	9407-9416	JJ	denotes	untreated
T7504	9417-9424	NNS	denotes	animals
T7505	9424-9425	.	denotes	.
T7506	9425-9574	sentence	denotes	Histological characterization of double transgenic (CaMKIIα-tTA × tet-APPswe/ind) mice revealed early-onset amyloid formation in all four new lines.
T7507	9426-9438	JJ	denotes	Histological
T7508	9439-9455	NN	denotes	characterization
T7509	9513-9521	VBD	denotes	revealed
T7510	9456-9458	IN	denotes	of
T7511	9459-9465	JJ	denotes	double
T7512	9508-9512	NNS	denotes	mice
T7513	9466-9476	JJ	denotes	transgenic
T7514	9477-9478	-LRB-	denotes	(
T7515	9496-9502	NN	denotes	APPswe
T7516	9478-9485	NN	denotes	CaMKIIα
T7517	9485-9486	HYPH	denotes	-
T7518	9486-9489	NN	denotes	tTA
T7519	9490-9491	SYM	denotes	×
T7520	9492-9495	NN	denotes	tet
T7521	9495-9496	HYPH	denotes	-
T7522	9502-9503	HYPH	denotes	/
T7523	9503-9506	JJ	denotes	ind
T7524	9506-9507	-RRB-	denotes	)
T7525	9522-9527	JJ	denotes	early
T7526	9528-9533	NN	denotes	onset
T7527	9527-9528	HYPH	denotes	-
T7528	9542-9551	NN	denotes	formation
T7529	9534-9541	NN	denotes	amyloid
T7530	9552-9554	IN	denotes	in
T7531	9555-9558	DT	denotes	all
T7532	9568-9573	NNS	denotes	lines
T7533	9559-9563	CD	denotes	four
T7534	9564-9567	JJ	denotes	new
T7535	9573-9574	.	denotes	.
T7536	9574-9650	sentence	denotes	Amyloid plaques were seen in mice as young as 8 wk of age (data not shown).
T7537	9575-9582	NN	denotes	Amyloid
T7538	9583-9590	NNS	denotes	plaques
T7539	9596-9600	VBN	denotes	seen
T7540	9591-9595	VBD	denotes	were
T7541	9601-9603	IN	denotes	in
T7542	9604-9608	NNS	denotes	mice
T7543	9609-9611	RB	denotes	as
T7544	9612-9617	JJ	denotes	young
T7545	9618-9620	IN	denotes	as
T7546	9621-9622	CD	denotes	8
T7547	9623-9625	NN	denotes	wk
T7548	9626-9628	IN	denotes	of
T7549	9629-9632	NN	denotes	age
T7550	9633-9634	-LRB-	denotes	(
T7551	9643-9648	VBN	denotes	shown
T7552	9634-9638	NNS	denotes	data
T7553	9639-9642	RB	denotes	not
T7554	9648-9649	-RRB-	denotes	)
T7555	9649-9650	.	denotes	.
T7556	9650-9829	sentence	denotes	Plaques were limited to the forebrain, including the cortex, hippocampus, olfactory bulb, and striatum, where the CaMKIIα promoter is known to be most active [16,28] (Figure S2).
T7557	9651-9658	NNS	denotes	Plaques
T7558	9664-9671	VBN	denotes	limited
T7559	9659-9663	VBD	denotes	were
T7560	9672-9674	IN	denotes	to
T7561	9675-9678	DT	denotes	the
T7562	9679-9688	NN	denotes	forebrain
T7563	9688-9690	,	denotes	,
T7564	9690-9699	VBG	denotes	including
T7565	9700-9703	DT	denotes	the
T7566	9704-9710	NN	denotes	cortex
T7567	9710-9712	,	denotes	,
T7568	9712-9723	NN	denotes	hippocampus
T7569	9723-9725	,	denotes	,
T7570	9725-9734	JJ	denotes	olfactory
T7571	9735-9739	NN	denotes	bulb
T7572	9739-9741	,	denotes	,
T7573	9741-9744	CC	denotes	and
T7574	9745-9753	NN	denotes	striatum
T7575	9753-9755	,	denotes	,
T7576	9755-9760	WRB	denotes	where
T7577	9785-9790	VBN	denotes	known
T7578	9761-9764	DT	denotes	the
T7579	9773-9781	NN	denotes	promoter
T7580	9765-9772	NN	denotes	CaMKIIα
T7581	9782-9784	VBZ	denotes	is
T7582	9791-9793	TO	denotes	to
T7583	9794-9796	VB	denotes	be
T7584	9797-9801	RBS	denotes	most
T7585	9802-9808	JJ	denotes	active
T7586	9809-9810	-LRB-	denotes	[
T7587	9813-9815	CD	denotes	28
T7588	9810-9812	CD	denotes	16
T7589	9812-9813	,	denotes	,
T7590	9815-9816	-RRB-	denotes	]
T7591	9817-9818	-LRB-	denotes	(
T7592	9825-9827	NN	denotes	S2
T7593	9818-9824	NN	denotes	Figure
T7594	9827-9828	-RRB-	denotes	)
T7595	9828-9829	.	denotes	.
T7596	9829-9939	sentence	denotes	By 6 mo of age, amyloid burden became severe, covering large areas of the cortex and hippocampus (Figure S3).
T7597	9830-9832	IN	denotes	By
T7598	9861-9867	VBD	denotes	became
T7599	9833-9834	CD	denotes	6
T7600	9835-9837	NN	denotes	mo
T7601	9838-9840	IN	denotes	of
T7602	9841-9844	NN	denotes	age
T7603	9844-9846	,	denotes	,
T7604	9846-9853	NN	denotes	amyloid
T7605	9854-9860	NN	denotes	burden
T7606	9868-9874	JJ	denotes	severe
T7607	9874-9876	,	denotes	,
T7608	9876-9884	VBG	denotes	covering
T7609	9885-9890	JJ	denotes	large
T7610	9891-9896	NNS	denotes	areas
T7611	9897-9899	IN	denotes	of
T7612	9900-9903	DT	denotes	the
T7613	9904-9910	NN	denotes	cortex
T7614	9911-9914	CC	denotes	and
T7615	9915-9926	NN	denotes	hippocampus
T7616	9927-9928	-LRB-	denotes	(
T7617	9935-9937	NN	denotes	S3
T7618	9928-9934	NN	denotes	Figure
T7619	9937-9938	-RRB-	denotes	)
T7620	9938-9939	.	denotes	.
T7621	9939-10068	sentence	denotes	No lesions were seen in the cerebellum or brain stem even at late ages, consistent with CaMKIIα-controlled transgene expression.
T7622	9940-9942	DT	denotes	No
T7623	9943-9950	NNS	denotes	lesions
T7624	9956-9960	VBN	denotes	seen
T7625	9951-9955	VBD	denotes	were
T7626	9961-9963	IN	denotes	in
T7627	9964-9967	DT	denotes	the
T7628	9968-9978	NN	denotes	cerebellum
T7629	9979-9981	CC	denotes	or
T7630	9982-9987	NN	denotes	brain
T7631	9988-9992	NN	denotes	stem
T7632	9993-9997	RB	denotes	even
T7633	9998-10000	IN	denotes	at
T7634	10001-10005	JJ	denotes	late
T7635	10006-10010	NNS	denotes	ages
T7636	10010-10012	,	denotes	,
T7637	10012-10022	JJ	denotes	consistent
T7638	10023-10027	IN	denotes	with
T7639	10028-10035	NN	denotes	CaMKIIα
T7640	10036-10046	VBN	denotes	controlled
T7641	10035-10036	HYPH	denotes	-
T7642	10057-10067	NN	denotes	expression
T7643	10047-10056	NN	denotes	transgene
T7644	10067-10068	.	denotes	.
T7645	10068-10202	sentence	denotes	Unlike what is thought to occur in the human disease, the first visible plaques in the tet-off APP mice are fibrillar-cored deposits.
T7646	10069-10075	IN	denotes	Unlike
T7647	10173-10176	VBP	denotes	are
T7648	10076-10080	WP	denotes	what
T7649	10084-10091	VBN	denotes	thought
T7650	10081-10083	VBZ	denotes	is
T7651	10092-10094	TO	denotes	to
T7652	10095-10100	VB	denotes	occur
T7653	10101-10103	IN	denotes	in
T7654	10104-10107	DT	denotes	the
T7655	10114-10121	NN	denotes	disease
T7656	10108-10113	JJ	denotes	human
T7657	10121-10123	,	denotes	,
T7658	10123-10126	DT	denotes	the
T7659	10141-10148	NNS	denotes	plaques
T7660	10127-10132	JJ	denotes	first
T7661	10133-10140	JJ	denotes	visible
T7662	10149-10151	IN	denotes	in
T7663	10152-10155	DT	denotes	the
T7664	10168-10172	NNS	denotes	mice
T7665	10156-10159	NN	denotes	tet
T7666	10160-10163	NN	denotes	off
T7667	10159-10160	HYPH	denotes	-
T7668	10164-10167	NN	denotes	APP
T7669	10177-10186	JJ	denotes	fibrillar
T7670	10187-10192	VBN	denotes	cored
T7671	10186-10187	HYPH	denotes	-
T7672	10193-10201	NNS	denotes	deposits
T7673	10201-10202	.	denotes	.
T7674	10202-10337	sentence	denotes	We have noted the same early appearance of cored deposits in other lines of APP transgenic mice that harbor the Swedish mutation [27].
T7675	10203-10205	PRP	denotes	We
T7676	10211-10216	VBN	denotes	noted
T7677	10206-10210	VBP	denotes	have
T7678	10217-10220	DT	denotes	the
T7679	10232-10242	NN	denotes	appearance
T7680	10221-10225	JJ	denotes	same
T7681	10226-10231	JJ	denotes	early
T7682	10243-10245	IN	denotes	of
T7683	10246-10251	VBN	denotes	cored
T7684	10252-10260	NNS	denotes	deposits
T7685	10261-10263	IN	denotes	in
T7686	10264-10269	JJ	denotes	other
T7687	10270-10275	NNS	denotes	lines
T7688	10276-10278	IN	denotes	of
T7689	10279-10282	NN	denotes	APP
T7690	10294-10298	NNS	denotes	mice
T7691	10283-10293	JJ	denotes	transgenic
T7692	10299-10303	WDT	denotes	that
T7693	10304-10310	VBP	denotes	harbor
T7694	10311-10314	DT	denotes	the
T7695	10323-10331	NN	denotes	mutation
T7696	10315-10322	JJ	denotes	Swedish
T7697	10332-10333	-LRB-	denotes	[
T7698	10333-10335	CD	denotes	27
T7699	10335-10336	-RRB-	denotes	]
T7700	10336-10337	.	denotes	.
T7701	10337-10440	sentence	denotes	Diffuse plaques were apparent in 6-mo-old tTA/APP mice, and became relatively abundant by 9 mo of age.
T7702	10338-10345	NN	denotes	Diffuse
T7703	10346-10353	NNS	denotes	plaques
T7704	10354-10358	VBD	denotes	were
T7705	10359-10367	JJ	denotes	apparent
T7706	10368-10370	IN	denotes	in
T7707	10371-10372	CD	denotes	6
T7708	10373-10375	NN	denotes	mo
T7709	10372-10373	HYPH	denotes	-
T7710	10376-10379	JJ	denotes	old
T7711	10375-10376	HYPH	denotes	-
T7712	10388-10392	NNS	denotes	mice
T7713	10380-10383	NN	denotes	tTA
T7714	10384-10387	NN	denotes	APP
T7715	10383-10384	HYPH	denotes	/
T7716	10392-10394	,	denotes	,
T7717	10394-10397	CC	denotes	and
T7718	10398-10404	VBD	denotes	became
T7719	10405-10415	RB	denotes	relatively
T7720	10416-10424	JJ	denotes	abundant
T7721	10425-10427	IN	denotes	by
T7722	10428-10429	CD	denotes	9
T7723	10430-10432	NN	denotes	mo
T7724	10433-10435	IN	denotes	of
T7725	10436-10439	NN	denotes	age
T7726	10439-10440	.	denotes	.
T7727	10440-10594	sentence	denotes	At older ages (9–12 mo) amyloid deposits were visible in the thalamus, which has also been observed in mice expressing mutant APP via the Thy-1 promoter.
T7728	10441-10443	IN	denotes	At
T7729	10482-10486	VBD	denotes	were
T7730	10444-10449	JJR	denotes	older
T7731	10450-10454	NNS	denotes	ages
T7732	10455-10456	-LRB-	denotes	(
T7733	10456-10457	CD	denotes	9
T7734	10458-10460	CD	denotes	12
T7735	10457-10458	SYM	denotes	–
T7736	10461-10463	NN	denotes	mo
T7737	10463-10464	-RRB-	denotes	)
T7738	10465-10472	NN	denotes	amyloid
T7739	10473-10481	NNS	denotes	deposits
T7740	10487-10494	JJ	denotes	visible
T7741	10495-10497	IN	denotes	in
T7742	10498-10501	DT	denotes	the
T7743	10502-10510	NN	denotes	thalamus
T7744	10510-10512	,	denotes	,
T7745	10512-10517	WDT	denotes	which
T7746	10532-10540	VBN	denotes	observed
T7747	10518-10521	VBZ	denotes	has
T7748	10522-10526	RB	denotes	also
T7749	10527-10531	VBN	denotes	been
T7750	10541-10543	IN	denotes	in
T7751	10544-10548	NNS	denotes	mice
T7752	10549-10559	VBG	denotes	expressing
T7753	10560-10566	NN	denotes	mutant
T7754	10567-10570	NN	denotes	APP
T7755	10571-10574	IN	denotes	via
T7756	10575-10578	DT	denotes	the
T7757	10585-10593	NN	denotes	promoter
T7758	10579-10582	NN	denotes	Thy
T7759	10582-10583	HYPH	denotes	-
T7760	10583-10584	CD	denotes	1
T7761	10593-10594	.	denotes	.
T7762	10594-10752	sentence	denotes	The presence of amyloid pathology in this region has been attributed to axonal transport of APP/Aβ to the terminals of cortical neurons in the thalamus [29].
T7763	10595-10598	DT	denotes	The
T7764	10599-10607	NN	denotes	presence
T7765	10653-10663	VBN	denotes	attributed
T7766	10608-10610	IN	denotes	of
T7767	10611-10618	NN	denotes	amyloid
T7768	10619-10628	NN	denotes	pathology
T7769	10629-10631	IN	denotes	in
T7770	10632-10636	DT	denotes	this
T7771	10637-10643	NN	denotes	region
T7772	10644-10647	VBZ	denotes	has
T7773	10648-10652	VBN	denotes	been
T7774	10664-10666	IN	denotes	to
T7775	10667-10673	JJ	denotes	axonal
T7776	10674-10683	NN	denotes	transport
T7777	10684-10686	IN	denotes	of
T7778	10687-10690	NN	denotes	APP
T7779	10691-10693	NN	denotes	Aβ
T7780	10690-10691	HYPH	denotes	/
T7781	10694-10696	IN	denotes	to
T7782	10697-10700	DT	denotes	the
T7783	10701-10710	NNS	denotes	terminals
T7784	10711-10713	IN	denotes	of
T7785	10714-10722	JJ	denotes	cortical
T7786	10723-10730	NNS	denotes	neurons
T7787	10731-10733	IN	denotes	in
T7788	10734-10737	DT	denotes	the
T7789	10738-10746	NN	denotes	thalamus
T7790	10747-10748	-LRB-	denotes	[
T7791	10748-10750	CD	denotes	29
T7792	10750-10751	-RRB-	denotes	]
T7793	10751-10752	.	denotes	.
T7794	10752-10870	sentence	denotes	Most importantly, only double transgenic mice, expressing both the tTA and APP transgenes, developed amyloid lesions.
T7795	10753-10757	RBS	denotes	Most
T7796	10758-10769	RB	denotes	importantly
T7797	10844-10853	VBD	denotes	developed
T7798	10769-10771	,	denotes	,
T7799	10771-10775	RB	denotes	only
T7800	10794-10798	NNS	denotes	mice
T7801	10776-10782	JJ	denotes	double
T7802	10783-10793	JJ	denotes	transgenic
T7803	10798-10800	,	denotes	,
T7804	10800-10810	VBG	denotes	expressing
T7805	10811-10815	CC	denotes	both
T7806	10832-10842	NNS	denotes	transgenes
T7807	10816-10819	DT	denotes	the
T7808	10820-10823	NN	denotes	tTA
T7809	10824-10827	CC	denotes	and
T7810	10828-10831	NN	denotes	APP
T7811	10842-10844	,	denotes	,
T7812	10854-10861	NN	denotes	amyloid
T7813	10862-10869	NNS	denotes	lesions
T7814	10869-10870	.	denotes	.
T7815	10870-10953	sentence	denotes	Single transgenic mice up to 15 mo of age showed no sign of pathology (Figure S3).
T7816	10871-10877	JJ	denotes	Single
T7817	10878-10888	JJ	denotes	transgenic
T7818	10889-10893	NNS	denotes	mice
T7819	10913-10919	VBD	denotes	showed
T7820	10894-10896	IN	denotes	up
T7821	10900-10902	CD	denotes	15
T7822	10897-10899	IN	denotes	to
T7823	10903-10905	NN	denotes	mo
T7824	10906-10908	IN	denotes	of
T7825	10909-10912	NN	denotes	age
T7826	10920-10922	DT	denotes	no
T7827	10923-10927	NN	denotes	sign
T7828	10928-10930	IN	denotes	of
T7829	10931-10940	NN	denotes	pathology
T7830	10941-10942	-LRB-	denotes	(
T7831	10949-10951	NN	denotes	S3
T7832	10942-10948	NN	denotes	Figure
T7833	10951-10952	-RRB-	denotes	)
T7834	10952-10953	.	denotes	.
T7835	10953-11063	sentence	denotes	Similarly, amyloid pathology can be completely prevented in double transgenic animals born and raised on dox.
T7836	10954-10963	RB	denotes	Similarly
T7837	11001-11010	VBN	denotes	prevented
T7838	10963-10965	,	denotes	,
T7839	10965-10972	NN	denotes	amyloid
T7840	10973-10982	NN	denotes	pathology
T7841	10983-10986	MD	denotes	can
T7842	10987-10989	VB	denotes	be
T7843	10990-11000	RB	denotes	completely
T7844	11011-11013	IN	denotes	in
T7845	11014-11020	JJ	denotes	double
T7846	11021-11031	JJ	denotes	transgenic
T7847	11032-11039	NNS	denotes	animals
T7848	11040-11044	VBN	denotes	born
T7849	11045-11048	CC	denotes	and
T7850	11049-11055	VBN	denotes	raised
T7851	11056-11058	IN	denotes	on
T7852	11059-11062	NN	denotes	dox
T7853	11062-11063	.	denotes	.
T7854	11063-11366	sentence	denotes	Animals from our highest expressing line (line 885) maintained on dox for up to 1 y harbored no amyloid pathology (data not shown), indicating that residual leakage of transgene expression in the presence of dox does not provide sufficient Aβ peptide to induce amyloid formation even over long periods.
T7855	11064-11071	NNS	denotes	Animals
T7856	11148-11156	VBD	denotes	harbored
T7857	11072-11076	IN	denotes	from
T7858	11077-11080	PRP$	denotes	our
T7859	11100-11104	NN	denotes	line
T7860	11081-11088	JJS	denotes	highest
T7861	11089-11099	VBG	denotes	expressing
T7862	11105-11106	-LRB-	denotes	(
T7863	11106-11110	NN	denotes	line
T7864	11111-11114	CD	denotes	885
T7865	11114-11115	-RRB-	denotes	)
T7866	11116-11126	VBN	denotes	maintained
T7867	11127-11129	IN	denotes	on
T7868	11130-11133	NN	denotes	dox
T7869	11134-11137	IN	denotes	for
T7870	11138-11140	IN	denotes	up
T7871	11141-11143	IN	denotes	to
T7872	11144-11145	CD	denotes	1
T7873	11146-11147	NN	denotes	y
T7874	11157-11159	DT	denotes	no
T7875	11168-11177	NN	denotes	pathology
T7876	11160-11167	NN	denotes	amyloid
T7877	11178-11179	-LRB-	denotes	(
T7878	11188-11193	VBN	denotes	shown
T7879	11179-11183	NNS	denotes	data
T7880	11184-11187	RB	denotes	not
T7881	11193-11194	-RRB-	denotes	)
T7882	11194-11196	,	denotes	,
T7883	11196-11206	VBG	denotes	indicating
T7884	11207-11211	IN	denotes	that
T7885	11285-11292	VB	denotes	provide
T7886	11212-11220	JJ	denotes	residual
T7887	11221-11228	NN	denotes	leakage
T7888	11229-11231	IN	denotes	of
T7889	11232-11241	NN	denotes	transgene
T7890	11242-11252	NN	denotes	expression
T7891	11253-11255	IN	denotes	in
T7892	11256-11259	DT	denotes	the
T7893	11260-11268	NN	denotes	presence
T7894	11269-11271	IN	denotes	of
T7895	11272-11275	NN	denotes	dox
T7896	11276-11280	VBZ	denotes	does
T7897	11281-11284	RB	denotes	not
T7898	11293-11303	JJ	denotes	sufficient
T7899	11307-11314	NN	denotes	peptide
T7900	11304-11306	NN	denotes	Aβ
T7901	11315-11317	TO	denotes	to
T7902	11318-11324	VB	denotes	induce
T7903	11325-11332	NN	denotes	amyloid
T7904	11333-11342	NN	denotes	formation
T7905	11343-11347	RB	denotes	even
T7906	11348-11352	IN	denotes	over
T7907	11353-11357	JJ	denotes	long
T7908	11358-11365	NNS	denotes	periods
T7909	11365-11366	.	denotes	.
T7910	11366-11615	sentence	denotes	To mimic therapeutic intervention with inhibitors of Aβ production, we raised a group of 25 double transgenic mice (CaMKIIα-tTA × APP line 107) on normal food until 6 mo of age, when we knew amyloid formation was already well underway in the brain.
T7911	11367-11369	TO	denotes	To
T7912	11370-11375	VB	denotes	mimic
T7913	11438-11444	VBD	denotes	raised
T7914	11376-11387	JJ	denotes	therapeutic
T7915	11388-11400	NN	denotes	intervention
T7916	11401-11405	IN	denotes	with
T7917	11406-11416	NNS	denotes	inhibitors
T7918	11417-11419	IN	denotes	of
T7919	11420-11422	NN	denotes	Aβ
T7920	11423-11433	NN	denotes	production
T7921	11433-11435	,	denotes	,
T7922	11435-11437	PRP	denotes	we
T7923	11445-11446	DT	denotes	a
T7924	11447-11452	NN	denotes	group
T7925	11453-11455	IN	denotes	of
T7926	11456-11458	CD	denotes	25
T7927	11477-11481	NNS	denotes	mice
T7928	11459-11465	JJ	denotes	double
T7929	11466-11476	JJ	denotes	transgenic
T7930	11482-11483	-LRB-	denotes	(
T7931	11497-11500	NN	denotes	APP
T7932	11483-11490	NN	denotes	CaMKIIα
T7933	11490-11491	HYPH	denotes	-
T7934	11491-11494	NN	denotes	tTA
T7935	11495-11496	SYM	denotes	×
T7936	11501-11505	NN	denotes	line
T7937	11506-11509	CD	denotes	107
T7938	11509-11510	-RRB-	denotes	)
T7939	11511-11513	IN	denotes	on
T7940	11514-11520	JJ	denotes	normal
T7941	11521-11525	NN	denotes	food
T7942	11526-11531	IN	denotes	until
T7943	11532-11533	CD	denotes	6
T7944	11534-11536	NN	denotes	mo
T7945	11537-11539	IN	denotes	of
T7946	11540-11543	NN	denotes	age
T7947	11543-11545	,	denotes	,
T7948	11545-11549	WRB	denotes	when
T7949	11553-11557	VBD	denotes	knew
T7950	11550-11552	PRP	denotes	we
T7951	11558-11565	NN	denotes	amyloid
T7952	11566-11575	NN	denotes	formation
T7953	11576-11579	VBD	denotes	was
T7954	11580-11587	RB	denotes	already
T7955	11588-11592	RB	denotes	well
T7956	11593-11601	JJ	denotes	underway
T7957	11602-11604	IN	denotes	in
T7958	11605-11608	DT	denotes	the
T7959	11609-11614	NN	denotes	brain
T7960	11614-11615	.	denotes	.
T7961	11615-11760	sentence	denotes	At 6 mo, half of the animals were switched from normal chow to food containing dox at 200 mg/kg until they were sacrificed at 9 or 12 mo of age.
T7962	11616-11618	IN	denotes	At
T7963	11650-11658	VBN	denotes	switched
T7964	11619-11620	CD	denotes	6
T7965	11621-11623	NN	denotes	mo
T7966	11623-11625	,	denotes	,
T7967	11625-11629	NN	denotes	half
T7968	11630-11632	IN	denotes	of
T7969	11633-11636	DT	denotes	the
T7970	11637-11644	NNS	denotes	animals
T7971	11645-11649	VBD	denotes	were
T7972	11659-11663	IN	denotes	from
T7973	11664-11670	JJ	denotes	normal
T7974	11671-11675	NN	denotes	chow
T7975	11676-11678	IN	denotes	to
T7976	11679-11683	NN	denotes	food
T7977	11684-11694	VBG	denotes	containing
T7978	11695-11698	NN	denotes	dox
T7979	11699-11701	IN	denotes	at
T7980	11702-11705	CD	denotes	200
T7981	11706-11708	NN	denotes	mg
T7982	11708-11709	SYM	denotes	/
T7983	11709-11711	NN	denotes	kg
T7984	11712-11717	IN	denotes	until
T7985	11728-11738	VBN	denotes	sacrificed
T7986	11718-11722	PRP	denotes	they
T7987	11723-11727	VBD	denotes	were
T7988	11739-11741	IN	denotes	at
T7989	11742-11743	CD	denotes	9
T7990	11750-11752	NN	denotes	mo
T7991	11744-11746	CC	denotes	or
T7992	11747-11749	CD	denotes	12
T7993	11753-11755	IN	denotes	of
T7994	11756-11759	NN	denotes	age
T7995	11759-11760	.	denotes	.
T7996	11760-11830	sentence	denotes	The remaining control animals were kept on standard chow (untreated).
T7997	11761-11764	DT	denotes	The
T7998	11783-11790	NNS	denotes	animals
T7999	11765-11774	VBG	denotes	remaining
T8000	11775-11782	NN	denotes	control
T8001	11796-11800	VBN	denotes	kept
T8002	11791-11795	VBD	denotes	were
T8003	11801-11803	IN	denotes	on
T8004	11804-11812	JJ	denotes	standard
T8005	11813-11817	NN	denotes	chow
T8006	11818-11819	-LRB-	denotes	(
T8007	11819-11828	JJ	denotes	untreated
T8008	11828-11829	-RRB-	denotes	)
T8009	11829-11830	.	denotes	.
T8010	11830-11975	sentence	denotes	In all, four cohorts were created: 6 mo untreated (n = 7), 9 mo untreated (n = 5), 6 mo + 3 mo treated (n = 8), and 6 mo + 6 mo treated (n = 5).
T8011	11831-11833	IN	denotes	In
T8012	11857-11864	VBN	denotes	created
T8013	11834-11837	DT	denotes	all
T8014	11837-11839	,	denotes	,
T8015	11839-11843	CD	denotes	four
T8016	11844-11851	NNS	denotes	cohorts
T8017	11852-11856	VBD	denotes	were
T8018	11864-11866	:	denotes	:
T8019	11866-11867	CD	denotes	6
T8020	11868-11870	NN	denotes	mo
T8021	11871-11880	JJ	denotes	untreated
T8022	11881-11882	-LRB-	denotes	(
T8023	11886-11887	CD	denotes	7
T8024	11882-11883	NN	denotes	n
T8025	11884-11885	SYM	denotes	=
T8026	11887-11888	-RRB-	denotes	)
T8027	11888-11890	,	denotes	,
T8028	11890-11891	CD	denotes	9
T8029	11892-11894	NN	denotes	mo
T8030	11895-11904	JJ	denotes	untreated
T8031	11905-11906	-LRB-	denotes	(
T8032	11910-11911	CD	denotes	5
T8033	11906-11907	NN	denotes	n
T8034	11908-11909	SYM	denotes	=
T8035	11911-11912	-RRB-	denotes	)
T8036	11912-11914	,	denotes	,
T8037	11914-11915	CD	denotes	6
T8038	11916-11918	NN	denotes	mo
T8039	11919-11920	SYM	denotes	+
T8040	11921-11922	CD	denotes	3
T8041	11923-11925	NN	denotes	mo
T8042	11926-11933	VBN	denotes	treated
T8043	11934-11935	-LRB-	denotes	(
T8044	11939-11940	CD	denotes	8
T8045	11935-11936	NN	denotes	n
T8046	11937-11938	SYM	denotes	=
T8047	11940-11941	-RRB-	denotes	)
T8048	11941-11943	,	denotes	,
T8049	11943-11946	CC	denotes	and
T8050	11947-11948	CD	denotes	6
T8051	11949-11951	NN	denotes	mo
T8052	11952-11953	SYM	denotes	+
T8053	11954-11955	CD	denotes	6
T8054	11956-11958	NN	denotes	mo
T8055	11959-11966	VBN	denotes	treated
T8056	11967-11968	-LRB-	denotes	(
T8057	11972-11973	CD	denotes	5
T8058	11968-11969	NN	denotes	n
T8059	11970-11971	SYM	denotes	=
T8060	11973-11974	-RRB-	denotes	)
T8061	11974-11975	.	denotes	.
T8062	11975-12098	sentence	denotes	Full suppression (>95%) of transgenic APPswe/ind levels in the dox-treated animals was confirmed by immunoblot (Figure 3).
T8063	11976-11980	JJ	denotes	Full
T8064	11981-11992	NN	denotes	suppression
T8065	12063-12072	VBN	denotes	confirmed
T8066	11993-11994	-LRB-	denotes	(
T8067	11997-11998	NN	denotes	%
T8068	11994-11995	SYM	denotes	>
T8069	11995-11997	CD	denotes	95
T8070	11998-11999	-RRB-	denotes	)
T8071	12000-12002	IN	denotes	of
T8072	12003-12013	JJ	denotes	transgenic
T8073	12025-12031	NNS	denotes	levels
T8074	12014-12020	NN	denotes	APPswe
T8075	12021-12024	NN	denotes	ind
T8076	12020-12021	HYPH	denotes	/
T8077	12032-12034	IN	denotes	in
T8078	12035-12038	DT	denotes	the
T8079	12051-12058	NNS	denotes	animals
T8080	12039-12042	NN	denotes	dox
T8081	12043-12050	VBN	denotes	treated
T8082	12042-12043	HYPH	denotes	-
T8083	12059-12062	VBD	denotes	was
T8084	12073-12075	IN	denotes	by
T8085	12076-12086	NN	denotes	immunoblot
T8086	12087-12088	-LRB-	denotes	(
T8087	12088-12094	NN	denotes	Figure
T8088	12095-12096	CD	denotes	3
T8089	12096-12097	-RRB-	denotes	)
T8090	12097-12098	.	denotes	.
T8091	12098-12325	sentence	denotes	To ensure that the transgene could be suppressed as rapidly in 6-mo-old mice with fulminant pathology as it can in young, predeposit animals, we treated an additional set of 6-mo-old animals with dox for 1 wk prior to harvest.
T8092	12099-12101	TO	denotes	To
T8093	12102-12108	VB	denotes	ensure
T8094	12244-12251	VBD	denotes	treated
T8095	12109-12113	IN	denotes	that
T8096	12137-12147	VBN	denotes	suppressed
T8097	12114-12117	DT	denotes	the
T8098	12118-12127	NN	denotes	transgene
T8099	12128-12133	MD	denotes	could
T8100	12134-12136	VB	denotes	be
T8101	12148-12150	RB	denotes	as
T8102	12151-12158	RB	denotes	rapidly
T8103	12159-12161	IN	denotes	in
T8104	12162-12163	CD	denotes	6
T8105	12164-12166	NN	denotes	mo
T8106	12163-12164	HYPH	denotes	-
T8107	12167-12170	JJ	denotes	old
T8108	12166-12167	HYPH	denotes	-
T8109	12171-12175	NNS	denotes	mice
T8110	12176-12180	IN	denotes	with
T8111	12181-12190	JJ	denotes	fulminant
T8112	12191-12200	NN	denotes	pathology
T8113	12201-12203	IN	denotes	as
T8114	12207-12210	MD	denotes	can
T8115	12204-12206	PRP	denotes	it
T8116	12211-12213	IN	denotes	in
T8117	12214-12219	JJ	denotes	young
T8118	12232-12239	NNS	denotes	animals
T8119	12219-12221	,	denotes	,
T8120	12221-12231	JJ	denotes	predeposit
T8121	12239-12241	,	denotes	,
T8122	12241-12243	PRP	denotes	we
T8123	12252-12254	DT	denotes	an
T8124	12266-12269	NN	denotes	set
T8125	12255-12265	JJ	denotes	additional
T8126	12270-12272	IN	denotes	of
T8127	12273-12274	CD	denotes	6
T8128	12275-12277	NN	denotes	mo
T8129	12274-12275	HYPH	denotes	-
T8130	12278-12281	JJ	denotes	old
T8131	12277-12278	HYPH	denotes	-
T8132	12282-12289	NNS	denotes	animals
T8133	12290-12294	IN	denotes	with
T8134	12295-12298	NN	denotes	dox
T8135	12299-12302	IN	denotes	for
T8136	12303-12304	CD	denotes	1
T8137	12305-12307	NN	denotes	wk
T8138	12308-12313	JJ	denotes	prior
T8139	12314-12316	IN	denotes	to
T8140	12317-12324	NN	denotes	harvest
T8141	12324-12325	.	denotes	.
T8142	12325-12566	sentence	denotes	Importantly, both APPswe/ind and APP–C-terminal fragment levels were fully suppressed after only 1 wk of treatment, indicating that the in vivo half-life of APPswe/ind and its processed C-terminal fragments are relatively short (Figure 3D).
T8143	12326-12337	RB	denotes	Importantly
T8144	12401-12411	VBN	denotes	suppressed
T8145	12337-12339	,	denotes	,
T8146	12339-12343	CC	denotes	both
T8147	12351-12354	NN	denotes	ind
T8148	12344-12350	NN	denotes	APPswe
T8149	12350-12351	HYPH	denotes	/
T8150	12365-12373	JJ	denotes	terminal
T8151	12355-12358	CC	denotes	and
T8152	12359-12362	NN	denotes	APP
T8153	12363-12364	NN	denotes	C
T8154	12362-12363	HYPH	denotes	–
T8155	12364-12365	HYPH	denotes	-
T8156	12383-12389	NNS	denotes	levels
T8157	12374-12382	NN	denotes	fragment
T8158	12390-12394	VBD	denotes	were
T8159	12395-12400	RB	denotes	fully
T8160	12412-12417	IN	denotes	after
T8161	12418-12422	RB	denotes	only
T8162	12425-12427	NN	denotes	wk
T8163	12423-12424	CD	denotes	1
T8164	12428-12430	IN	denotes	of
T8165	12431-12440	NN	denotes	treatment
T8166	12440-12442	,	denotes	,
T8167	12442-12452	VBG	denotes	indicating
T8168	12453-12457	IN	denotes	that
T8169	12533-12536	VBP	denotes	are
T8170	12458-12461	DT	denotes	the
T8171	12475-12479	NN	denotes	life
T8172	12462-12464	FW	denotes	in
T8173	12465-12469	FW	denotes	vivo
T8174	12470-12474	NN	denotes	half
T8175	12474-12475	HYPH	denotes	-
T8176	12480-12482	IN	denotes	of
T8177	12483-12489	NN	denotes	APPswe
T8178	12490-12493	NN	denotes	ind
T8179	12489-12490	HYPH	denotes	/
T8180	12494-12497	CC	denotes	and
T8181	12498-12501	PRP$	denotes	its
T8182	12523-12532	NNS	denotes	fragments
T8183	12502-12511	VBN	denotes	processed
T8184	12512-12513	NN	denotes	C
T8185	12514-12522	JJ	denotes	terminal
T8186	12513-12514	HYPH	denotes	-
T8187	12537-12547	RB	denotes	relatively
T8188	12548-12553	JJ	denotes	short
T8189	12554-12555	-LRB-	denotes	(
T8190	12562-12564	NN	denotes	3D
T8191	12555-12561	NN	denotes	Figure
T8192	12564-12565	-RRB-	denotes	)
T8193	12565-12566	.	denotes	.
T8194	12566-14495	sentence	denotes	Figure 3 Robust Transgene Suppression in Older Mice with Preexisting Amyloid Pathology (A) Cortical homogenates from 6- to 12-mo-old animals used for pathology studies described below (line 107) were immunoblotted with human-specific antibody 6E10 to examine transgene suppression following 3 or 6 mo of dox treatment. The blot was co-immunostained for endogenous superoxide dismutase 1 (SOD1) as a control for loading. (B) Quantitation of signal intensity from the Western blot shown in (A). Transgenic APP levels are significantly suppressed following 3 or 6 mo of dox treatment (96.9% and 97.6%, respectively). *, p < 0.001 compared to 6-mo-old untreated animals, Tukey post-hoc test applied to significant effect of group ANOVA F 3,12 = 107.22, p < 0.001. These data demonstrate that strong transgene suppression is attained both before and after the onset of amyloid pathology (see Figure 1 for predeposit experiments). (C) Experimental design. To examine the effects of chronic Aβ suppression on amyloid pathology after the onset of deposition, we compared untreated controls harvested at 6 and 9 mo of age to animals placed on dox at 6 mo of age and harvested after 3 or 6 mo of treatment. (D) Dox treatment leads to rapid transgene suppression even in 6-mo-old tTA/APP mice. Immunostaining with 6E10 shows APPswe/ind levels are dramatically reduced in 6-mo-old mice treated for 1 wk with dox (upper panel). A separate blot was immunostained for APP C-terminal fragments with CT15 antibody to show that the precursors to Aβ cleavage are decreased in parallel with the full-length protein (middle panel). Costaining for superoxide dismutase 1 was used as an internal control for loading (lower panel, taken from bottom half of 6E10 blot). Tissue sections from each animal in the four treatment groups were stained for amyloid pathology by Hirano silver, Campbell-Switzer silver, thioflavin-S, and Aβ immunohistochemistry.
T8195	14313-14319	NN	denotes	Tissue
T8196	14320-14328	NNS	denotes	sections
T8197	14380-14387	VBN	denotes	stained
T8198	14329-14333	IN	denotes	from
T8199	14334-14338	DT	denotes	each
T8200	14339-14345	NN	denotes	animal
T8201	14346-14348	IN	denotes	in
T8202	14349-14352	DT	denotes	the
T8203	14368-14374	NNS	denotes	groups
T8204	14353-14357	CD	denotes	four
T8205	14358-14367	NN	denotes	treatment
T8206	14375-14379	VBD	denotes	were
T8207	14388-14391	IN	denotes	for
T8208	14392-14399	NN	denotes	amyloid
T8209	14400-14409	NN	denotes	pathology
T8210	14410-14412	IN	denotes	by
T8211	14413-14419	NNP	denotes	Hirano
T8212	14420-14426	NN	denotes	silver
T8213	14426-14428	,	denotes	,
T8214	14428-14436	NNP	denotes	Campbell
T8215	14437-14444	NNP	denotes	Switzer
T8216	14436-14437	HYPH	denotes	-
T8217	14445-14451	NN	denotes	silver
T8218	14451-14453	,	denotes	,
T8219	14453-14463	NN	denotes	thioflavin
T8220	14464-14465	NN	denotes	S
T8221	14463-14464	HYPH	denotes	-
T8222	14465-14467	,	denotes	,
T8223	14467-14470	CC	denotes	and
T8224	14471-14473	NN	denotes	Aβ
T8225	14474-14494	NN	denotes	immunohistochemistry
T8226	14494-14495	.	denotes	.
T8227	14495-14641	sentence	denotes	As expected, the 6 mo untreated cohort displayed moderate amyloid pathology, and the 9 mo untreated cohort progressed to a severe amyloid burden.
T8228	14496-14498	IN	denotes	As
T8229	14499-14507	VBN	denotes	expected
T8230	14535-14544	VBD	denotes	displayed
T8231	14507-14509	,	denotes	,
T8232	14509-14512	DT	denotes	the
T8233	14528-14534	NN	denotes	cohort
T8234	14513-14514	CD	denotes	6
T8235	14515-14517	NN	denotes	mo
T8236	14518-14527	JJ	denotes	untreated
T8237	14545-14553	JJ	denotes	moderate
T8238	14562-14571	NN	denotes	pathology
T8239	14554-14561	NN	denotes	amyloid
T8240	14571-14573	,	denotes	,
T8241	14573-14576	CC	denotes	and
T8242	14577-14580	DT	denotes	the
T8243	14596-14602	NN	denotes	cohort
T8244	14581-14582	CD	denotes	9
T8245	14583-14585	NN	denotes	mo
T8246	14586-14595	JJ	denotes	untreated
T8247	14603-14613	VBD	denotes	progressed
T8248	14614-14616	IN	denotes	to
T8249	14617-14618	DT	denotes	a
T8250	14634-14640	NN	denotes	burden
T8251	14619-14625	JJ	denotes	severe
T8252	14626-14633	NN	denotes	amyloid
T8253	14640-14641	.	denotes	.
T8254	14641-14914	sentence	denotes	In contrast, the extent of amyloid pathology in mice from the 6 mo + 3 mo treated or 6 mo + 6 mo treated cohorts closely resembled that of the 6 mo untreated cohort, despite the significant age difference between the treated and untreated groups (Figures 4 and Figure S3).
T8255	14642-14644	IN	denotes	In
T8256	14763-14772	VBD	denotes	resembled
T8257	14645-14653	NN	denotes	contrast
T8258	14653-14655	,	denotes	,
T8259	14655-14658	DT	denotes	the
T8260	14659-14665	NN	denotes	extent
T8261	14666-14668	IN	denotes	of
T8262	14669-14676	NN	denotes	amyloid
T8263	14677-14686	NN	denotes	pathology
T8264	14687-14689	IN	denotes	in
T8265	14690-14694	NNS	denotes	mice
T8266	14695-14699	IN	denotes	from
T8267	14700-14703	DT	denotes	the
T8268	14747-14754	NNS	denotes	cohorts
T8269	14704-14705	CD	denotes	6
T8270	14706-14708	NN	denotes	mo
T8271	14716-14723	VBN	denotes	treated
T8272	14709-14710	SYM	denotes	+
T8273	14711-14712	CD	denotes	3
T8274	14713-14715	NN	denotes	mo
T8275	14724-14726	CC	denotes	or
T8276	14727-14728	CD	denotes	6
T8277	14729-14731	NN	denotes	mo
T8278	14739-14746	VBN	denotes	treated
T8279	14732-14733	SYM	denotes	+
T8280	14734-14735	CD	denotes	6
T8281	14736-14738	NN	denotes	mo
T8282	14755-14762	RB	denotes	closely
T8283	14773-14777	DT	denotes	that
T8284	14778-14780	IN	denotes	of
T8285	14781-14784	DT	denotes	the
T8286	14800-14806	NN	denotes	cohort
T8287	14785-14786	CD	denotes	6
T8288	14787-14789	NN	denotes	mo
T8289	14790-14799	JJ	denotes	untreated
T8290	14806-14808	,	denotes	,
T8291	14808-14815	IN	denotes	despite
T8292	14816-14819	DT	denotes	the
T8293	14836-14846	NN	denotes	difference
T8294	14820-14831	JJ	denotes	significant
T8295	14832-14835	NN	denotes	age
T8296	14847-14854	IN	denotes	between
T8297	14855-14858	DT	denotes	the
T8298	14881-14887	NNS	denotes	groups
T8299	14859-14866	VBN	denotes	treated
T8300	14867-14870	CC	denotes	and
T8301	14871-14880	JJ	denotes	untreated
T8302	14888-14889	-LRB-	denotes	(
T8303	14889-14896	NNS	denotes	Figures
T8304	14897-14898	CD	denotes	4
T8305	14899-14902	CC	denotes	and
T8306	14903-14909	NN	denotes	Figure
T8307	14910-14912	NN	denotes	S3
T8308	14912-14913	-RRB-	denotes	)
T8309	14913-14914	.	denotes	.
T8310	14914-15086	sentence	denotes	Well-formed plaques remained in the treated animals after 6 mo of transgene suppression, even though as much time was given to clear the lesions as they had taken to form.
T8311	14915-14919	RB	denotes	Well
T8312	14920-14926	VBN	denotes	formed
T8313	14919-14920	HYPH	denotes	-
T8314	14927-14934	NNS	denotes	plaques
T8315	14935-14943	VBD	denotes	remained
T8316	14944-14946	IN	denotes	in
T8317	14947-14950	DT	denotes	the
T8318	14959-14966	NNS	denotes	animals
T8319	14951-14958	VBN	denotes	treated
T8320	14967-14972	IN	denotes	after
T8321	14973-14974	CD	denotes	6
T8322	14975-14977	NN	denotes	mo
T8323	14978-14980	IN	denotes	of
T8324	14981-14990	NN	denotes	transgene
T8325	14991-15002	NN	denotes	suppression
T8326	15002-15004	,	denotes	,
T8327	15004-15008	RB	denotes	even
T8328	15033-15038	VBN	denotes	given
T8329	15009-15015	IN	denotes	though
T8330	15016-15018	RB	denotes	as
T8331	15019-15023	JJ	denotes	much
T8332	15024-15028	NN	denotes	time
T8333	15029-15032	VBD	denotes	was
T8334	15039-15041	TO	denotes	to
T8335	15042-15047	VB	denotes	clear
T8336	15048-15051	DT	denotes	the
T8337	15052-15059	NNS	denotes	lesions
T8338	15060-15062	IN	denotes	as
T8339	15072-15077	VBN	denotes	taken
T8340	15063-15067	PRP	denotes	they
T8341	15068-15071	VBD	denotes	had
T8342	15078-15080	TO	denotes	to
T8343	15081-15085	VB	denotes	form
T8344	15085-15086	.	denotes	.
T8345	15086-15180	sentence	denotes	Moreover, both types of amyloid, diffuse and fibrillar, remained intact throughout treatment.
T8346	15087-15095	RB	denotes	Moreover
T8347	15143-15151	VBD	denotes	remained
T8348	15095-15097	,	denotes	,
T8349	15097-15101	DT	denotes	both
T8350	15102-15107	NNS	denotes	types
T8351	15108-15110	IN	denotes	of
T8352	15111-15118	NN	denotes	amyloid
T8353	15118-15120	,	denotes	,
T8354	15120-15127	JJ	denotes	diffuse
T8355	15128-15131	CC	denotes	and
T8356	15132-15141	JJ	denotes	fibrillar
T8357	15141-15143	,	denotes	,
T8358	15152-15158	JJ	denotes	intact
T8359	15159-15169	IN	denotes	throughout
T8360	15170-15179	NN	denotes	treatment
T8361	15179-15180	.	denotes	.
T8362	15180-15338	sentence	denotes	Using the Campbell-Switzer silver stain to distinguish different forms of amyloid, we found diffuse plaques were as persistent as cored deposits (Figure S4).
T8363	15181-15186	VBG	denotes	Using
T8364	15267-15272	VBD	denotes	found
T8365	15187-15190	DT	denotes	the
T8366	15215-15220	NN	denotes	stain
T8367	15191-15199	NNP	denotes	Campbell
T8368	15200-15207	NNP	denotes	Switzer
T8369	15199-15200	HYPH	denotes	-
T8370	15208-15214	NN	denotes	silver
T8371	15221-15223	TO	denotes	to
T8372	15224-15235	VB	denotes	distinguish
T8373	15236-15245	JJ	denotes	different
T8374	15246-15251	NNS	denotes	forms
T8375	15252-15254	IN	denotes	of
T8376	15255-15262	NN	denotes	amyloid
T8377	15262-15264	,	denotes	,
T8378	15264-15266	PRP	denotes	we
T8379	15273-15280	JJ	denotes	diffuse
T8380	15281-15288	NNS	denotes	plaques
T8381	15289-15293	VBD	denotes	were
T8382	15294-15296	RB	denotes	as
T8383	15297-15307	JJ	denotes	persistent
T8384	15308-15310	IN	denotes	as
T8385	15311-15316	VBN	denotes	cored
T8386	15317-15325	NNS	denotes	deposits
T8387	15326-15327	-LRB-	denotes	(
T8388	15334-15336	NN	denotes	S4
T8389	15327-15333	NN	denotes	Figure
T8390	15336-15337	-RRB-	denotes	)
T8391	15337-15338	.	denotes	.
T8392	15338-15463	sentence	denotes	It was nevertheless clear that dox-induced suppression of transgenic APP had completely halted the progression of pathology.
T8393	15339-15341	PRP	denotes	It
T8394	15342-15345	VBD	denotes	was
T8395	15346-15358	RB	denotes	nevertheless
T8396	15359-15364	JJ	denotes	clear
T8397	15365-15369	IN	denotes	that
T8398	15427-15433	VBN	denotes	halted
T8399	15370-15373	NN	denotes	dox
T8400	15374-15381	VBN	denotes	induced
T8401	15373-15374	HYPH	denotes	-
T8402	15382-15393	NN	denotes	suppression
T8403	15394-15396	IN	denotes	of
T8404	15397-15407	JJ	denotes	transgenic
T8405	15408-15411	NN	denotes	APP
T8406	15412-15415	VBD	denotes	had
T8407	15416-15426	RB	denotes	completely
T8408	15434-15437	DT	denotes	the
T8409	15438-15449	NN	denotes	progression
T8410	15450-15452	IN	denotes	of
T8411	15453-15462	NN	denotes	pathology
T8412	15462-15463	.	denotes	.
T8413	15463-17367	sentence	denotes	Figure 4 Suppression of Transgenic APP Arrests Progression of Amyloid Pathology (A) Aggregated Aβ was quantified in cortical tissue from dox-treated and control tTA/APP mice (line 107) using a filter trap assay. Serial dilutions of protein homogenate were passed through a cellulose acetate filter; protein aggregates larger than the pore size were trapped and immunostained for Aβ. (B) Quantitation of signal intensity in the linear range of each filter trap dilution series (arrow in [A]) was used to compare aggregate load across treatment groups. Aggregated Aβ increased significantly between 6 and 9 mo of age in untreated mice (significant effect of group ANOVA F 3,18 = 7.85, p < 0.002). This progression of pathology was completely prevented by transgene suppression. The amount of aggregated Aβ was identical in untreated mice at 6 mo of age to that in 9- or 12-mo-old animals treated with dox (p > 0.9, Tukey post-hoc test). Single transgenic tTA samples were included as negative controls and showed no signal above background. , p < 0.01; , p < 0.005 versus 9-mo-old untreated mice, Tukey post-hoc test; **, p < 0.001 versus 9-mo-old untreated mice, Student's t-test. (C) Amyloid pathology in the hippocampus of representative mice from each treatment group: Hirano silver stain (top row), thioflavin-S (middle row), and Aβ immunohistochemistry (bottom row). Amyloid burden increases dramatically between 6 and 9 mo of age in untreated animals, but remains stable in transgene-suppressed mice over the same period (6 mo + 3 mo dox and 6 mo + 6 mo dox). Single transgenic animals (tTA only shown here) show no sign of amyloid pathology at any age tested. To confirm that the arrest of plaques without any sign of clearance was not unique to the line 107 mice, we repeated the dox-suppression experiment in a second line of tet-off APP mice (CaMKIIα-tTA × tet-APPswe/ind line 18; n = 22).
T8414	17135-17137	TO	denotes	To
T8415	17138-17145	VB	denotes	confirm
T8416	17243-17251	VBD	denotes	repeated
T8417	17146-17150	IN	denotes	that
T8418	17203-17206	VBD	denotes	was
T8419	17151-17154	DT	denotes	the
T8420	17155-17161	NN	denotes	arrest
T8421	17162-17164	IN	denotes	of
T8422	17165-17172	NNS	denotes	plaques
T8423	17173-17180	IN	denotes	without
T8424	17181-17184	DT	denotes	any
T8425	17185-17189	NN	denotes	sign
T8426	17190-17192	IN	denotes	of
T8427	17193-17202	NN	denotes	clearance
T8428	17207-17210	RB	denotes	not
T8429	17211-17217	JJ	denotes	unique
T8430	17218-17220	IN	denotes	to
T8431	17221-17224	DT	denotes	the
T8432	17234-17238	NNS	denotes	mice
T8433	17225-17229	NN	denotes	line
T8434	17230-17233	CD	denotes	107
T8435	17238-17240	,	denotes	,
T8436	17240-17242	PRP	denotes	we
T8437	17252-17255	DT	denotes	the
T8438	17272-17282	NN	denotes	experiment
T8439	17256-17259	NN	denotes	dox
T8440	17260-17271	NN	denotes	suppression
T8441	17259-17260	HYPH	denotes	-
T8442	17283-17285	IN	denotes	in
T8443	17286-17287	DT	denotes	a
T8444	17295-17299	NN	denotes	line
T8445	17288-17294	JJ	denotes	second
T8446	17300-17302	IN	denotes	of
T8447	17303-17306	NN	denotes	tet
T8448	17307-17310	NN	denotes	off
T8449	17306-17307	HYPH	denotes	-
T8450	17315-17319	NNS	denotes	mice
T8451	17311-17314	NN	denotes	APP
T8452	17320-17321	-LRB-	denotes	(
T8453	17350-17354	NN	denotes	line
T8454	17321-17328	NN	denotes	CaMKIIα
T8455	17346-17349	NN	denotes	ind
T8456	17328-17329	HYPH	denotes	-
T8457	17329-17332	NN	denotes	tTA
T8458	17333-17334	SYM	denotes	×
T8459	17335-17338	NN	denotes	tet
T8460	17338-17339	HYPH	denotes	-
T8461	17339-17345	NN	denotes	APPswe
T8462	17345-17346	HYPH	denotes	/
T8463	17355-17357	CD	denotes	18
T8464	17357-17358	:	denotes	;
T8465	17359-17360	NN	denotes	n
T8466	17363-17365	CD	denotes	22
T8467	17361-17362	SYM	denotes	=
T8468	17365-17366	-RRB-	denotes	)
T8469	17366-17367	.	denotes	.
T8470	17367-17560	sentence	denotes	Again, long-term dox treatment was begun at 6 mo of age, and mice were harvested after 3 mo of transgene suppression (6 mo untreated, n = 8; 9 mo untreated, n = 6; 6 mo + 3 mo treated, n = 8).
T8471	17368-17373	RB	denotes	Again
T8472	17403-17408	VBN	denotes	begun
T8473	17373-17375	,	denotes	,
T8474	17375-17379	JJ	denotes	long
T8475	17380-17384	NN	denotes	term
T8476	17379-17380	HYPH	denotes	-
T8477	17389-17398	NN	denotes	treatment
T8478	17385-17388	NN	denotes	dox
T8479	17399-17402	VBD	denotes	was
T8480	17409-17411	IN	denotes	at
T8481	17412-17413	CD	denotes	6
T8482	17414-17416	NN	denotes	mo
T8483	17417-17419	IN	denotes	of
T8484	17420-17423	NN	denotes	age
T8485	17423-17425	,	denotes	,
T8486	17425-17428	CC	denotes	and
T8487	17429-17433	NNS	denotes	mice
T8488	17439-17448	VBN	denotes	harvested
T8489	17434-17438	VBD	denotes	were
T8490	17449-17454	IN	denotes	after
T8491	17455-17456	CD	denotes	3
T8492	17457-17459	NN	denotes	mo
T8493	17460-17462	IN	denotes	of
T8494	17463-17472	NN	denotes	transgene
T8495	17473-17484	NN	denotes	suppression
T8496	17485-17486	-LRB-	denotes	(
T8497	17557-17558	CD	denotes	8
T8498	17486-17487	CD	denotes	6
T8499	17488-17490	NN	denotes	mo
T8500	17491-17500	JJ	denotes	untreated
T8501	17506-17507	CD	denotes	8
T8502	17500-17502	,	denotes	,
T8503	17502-17503	NN	denotes	n
T8504	17504-17505	SYM	denotes	=
T8505	17507-17508	:	denotes	;
T8506	17509-17510	CD	denotes	9
T8507	17511-17513	NN	denotes	mo
T8508	17514-17523	JJ	denotes	untreated
T8509	17529-17530	CD	denotes	6
T8510	17523-17525	,	denotes	,
T8511	17525-17526	NN	denotes	n
T8512	17527-17528	SYM	denotes	=
T8513	17530-17531	:	denotes	;
T8514	17532-17533	CD	denotes	6
T8515	17541-17543	NN	denotes	mo
T8516	17534-17536	NN	denotes	mo
T8517	17537-17538	SYM	denotes	+
T8518	17539-17540	CD	denotes	3
T8519	17544-17551	VBN	denotes	treated
T8520	17551-17553	,	denotes	,
T8521	17553-17554	NN	denotes	n
T8522	17555-17556	SYM	denotes	=
T8523	17558-17559	-RRB-	denotes	)
T8524	17559-17560	.	denotes	.
T8525	17560-17656	sentence	denotes	Immunoblotting for APP confirmed full transgene suppression in the treated animals (Figure S5).
T8526	17561-17575	NN	denotes	Immunoblotting
T8527	17584-17593	VBD	denotes	confirmed
T8528	17576-17579	IN	denotes	for
T8529	17580-17583	NN	denotes	APP
T8530	17594-17598	JJ	denotes	full
T8531	17609-17620	NN	denotes	suppression
T8532	17599-17608	NN	denotes	transgene
T8533	17621-17623	IN	denotes	in
T8534	17624-17627	DT	denotes	the
T8535	17636-17643	NNS	denotes	animals
T8536	17628-17635	VBN	denotes	treated
T8537	17644-17645	-LRB-	denotes	(
T8538	17652-17654	NN	denotes	S5
T8539	17645-17651	NN	denotes	Figure
T8540	17654-17655	-RRB-	denotes	)
T8541	17655-17656	.	denotes	.
T8542	17656-17784	sentence	denotes	As in the line 107 mice described above, amyloid burden worsened substantially in the untreated mice between 6 and 9 mo of age.
T8543	17657-17659	IN	denotes	As
T8544	17713-17721	VBD	denotes	worsened
T8545	17660-17662	IN	denotes	in
T8546	17663-17666	DT	denotes	the
T8547	17676-17680	NNS	denotes	mice
T8548	17667-17671	NN	denotes	line
T8549	17672-17675	CD	denotes	107
T8550	17681-17690	VBN	denotes	described
T8551	17691-17696	RB	denotes	above
T8552	17696-17698	,	denotes	,
T8553	17698-17705	NN	denotes	amyloid
T8554	17706-17712	NN	denotes	burden
T8555	17722-17735	RB	denotes	substantially
T8556	17736-17738	IN	denotes	in
T8557	17739-17742	DT	denotes	the
T8558	17753-17757	NNS	denotes	mice
T8559	17743-17752	JJ	denotes	untreated
T8560	17758-17765	IN	denotes	between
T8561	17766-17767	CD	denotes	6
T8562	17774-17776	NN	denotes	mo
T8563	17768-17771	CC	denotes	and
T8564	17772-17773	CD	denotes	9
T8565	17777-17779	IN	denotes	of
T8566	17780-17783	NN	denotes	age
T8567	17783-17784	.	denotes	.
T8568	17784-17917	sentence	denotes	Suppression of transgene expression abruptly arrested progression of pathology (Figure S6), but again without any sign of reduction.
T8569	17785-17796	NN	denotes	Suppression
T8570	17830-17838	VBD	denotes	arrested
T8571	17797-17799	IN	denotes	of
T8572	17800-17809	NN	denotes	transgene
T8573	17810-17820	NN	denotes	expression
T8574	17821-17829	RB	denotes	abruptly
T8575	17839-17850	NN	denotes	progression
T8576	17851-17853	IN	denotes	of
T8577	17854-17863	NN	denotes	pathology
T8578	17864-17865	-LRB-	denotes	(
T8579	17872-17874	NN	denotes	S6
T8580	17865-17871	NN	denotes	Figure
T8581	17874-17875	-RRB-	denotes	)
T8582	17875-17877	,	denotes	,
T8583	17877-17880	CC	denotes	but
T8584	17887-17894	IN	denotes	without
T8585	17881-17886	RB	denotes	again
T8586	17895-17898	DT	denotes	any
T8587	17899-17903	NN	denotes	sign
T8588	17904-17906	IN	denotes	of
T8589	17907-17916	NN	denotes	reduction
T8590	17916-17917	.	denotes	.
T8591	17917-18021	sentence	denotes	Both silver- and thioflavin-S-positive plaques could still be found in each of the dox-treated animals.
T8592	17918-17922	CC	denotes	Both
T8593	17923-17929	NN	denotes	silver
T8594	17948-17956	JJ	denotes	positive
T8595	17929-17930	HYPH	denotes	-
T8596	17931-17934	CC	denotes	and
T8597	17935-17945	NN	denotes	thioflavin
T8598	17946-17947	NN	denotes	S
T8599	17945-17946	HYPH	denotes	-
T8600	17947-17948	HYPH	denotes	-
T8601	17957-17964	NNS	denotes	plaques
T8602	17980-17985	VBN	denotes	found
T8603	17965-17970	MD	denotes	could
T8604	17971-17976	RB	denotes	still
T8605	17977-17979	VB	denotes	be
T8606	17986-17988	IN	denotes	in
T8607	17989-17993	DT	denotes	each
T8608	17994-17996	IN	denotes	of
T8609	17997-18000	DT	denotes	the
T8610	18013-18020	NNS	denotes	animals
T8611	18001-18004	NN	denotes	dox
T8612	18005-18012	VBN	denotes	treated
T8613	18004-18005	HYPH	denotes	-
T8614	18020-18021	.	denotes	.
T8615	18021-18204	sentence	denotes	We biochemically measured the amount of aggregated Aβ in the brains of our mice before and after transgene suppression using filter trap analysis of cortical tissue from each animal.
T8616	18022-18024	PRP	denotes	We
T8617	18039-18047	VBD	denotes	measured
T8618	18025-18038	RB	denotes	biochemically
T8619	18048-18051	DT	denotes	the
T8620	18052-18058	NN	denotes	amount
T8621	18059-18061	IN	denotes	of
T8622	18062-18072	VBN	denotes	aggregated
T8623	18073-18075	NN	denotes	Aβ
T8624	18076-18078	IN	denotes	in
T8625	18079-18082	DT	denotes	the
T8626	18083-18089	NNS	denotes	brains
T8627	18090-18092	IN	denotes	of
T8628	18093-18096	PRP$	denotes	our
T8629	18097-18101	NNS	denotes	mice
T8630	18102-18108	IN	denotes	before
T8631	18109-18112	CC	denotes	and
T8632	18113-18118	IN	denotes	after
T8633	18119-18128	NN	denotes	transgene
T8634	18129-18140	NN	denotes	suppression
T8635	18141-18146	VBG	denotes	using
T8636	18147-18153	NN	denotes	filter
T8637	18154-18158	NN	denotes	trap
T8638	18159-18167	NN	denotes	analysis
T8639	18168-18170	IN	denotes	of
T8640	18171-18179	JJ	denotes	cortical
T8641	18180-18186	NN	denotes	tissue
T8642	18187-18191	IN	denotes	from
T8643	18192-18196	DT	denotes	each
T8644	18197-18203	NN	denotes	animal
T8645	18203-18204	.	denotes	.
T8646	18204-18427	sentence	denotes	In this assay, serial dilutions of protein homogenate are passed through a cellulose acetate filter; particles larger than the pore size of the filter become trapped in the membrane and are revealed by immunoblotting [22].
T8647	18205-18207	IN	denotes	In
T8648	18263-18269	VBN	denotes	passed
T8649	18208-18212	DT	denotes	this
T8650	18213-18218	NN	denotes	assay
T8651	18218-18220	,	denotes	,
T8652	18220-18226	JJ	denotes	serial
T8653	18227-18236	NNS	denotes	dilutions
T8654	18237-18239	IN	denotes	of
T8655	18240-18247	NN	denotes	protein
T8656	18248-18258	NN	denotes	homogenate
T8657	18259-18262	VBP	denotes	are
T8658	18356-18362	VBP	denotes	become
T8659	18270-18277	IN	denotes	through
T8660	18278-18279	DT	denotes	a
T8661	18298-18304	NN	denotes	filter
T8662	18280-18289	NN	denotes	cellulose
T8663	18290-18297	NN	denotes	acetate
T8664	18304-18305	:	denotes	;
T8665	18306-18315	NNS	denotes	particles
T8666	18316-18322	JJR	denotes	larger
T8667	18323-18327	IN	denotes	than
T8668	18328-18331	DT	denotes	the
T8669	18337-18341	NN	denotes	size
T8670	18332-18336	NN	denotes	pore
T8671	18342-18344	IN	denotes	of
T8672	18345-18348	DT	denotes	the
T8673	18349-18355	NN	denotes	filter
T8674	18363-18370	JJ	denotes	trapped
T8675	18371-18373	IN	denotes	in
T8676	18374-18377	DT	denotes	the
T8677	18378-18386	NN	denotes	membrane
T8678	18387-18390	CC	denotes	and
T8679	18391-18394	VBP	denotes	are
T8680	18395-18403	VBN	denotes	revealed
T8681	18404-18406	IN	denotes	by
T8682	18407-18421	NN	denotes	immunoblotting
T8683	18422-18423	-LRB-	denotes	[
T8684	18423-18425	CD	denotes	22
T8685	18425-18426	-RRB-	denotes	]
T8686	18426-18427	.	denotes	.
T8687	18427-18649	sentence	denotes	Consistent with our visual analysis of the histological sections, line 107 tTA/APP mice treated with dox for 3 or 6 mo had the same amount of aggregated Aβ as when they started treatment at 6 mo of age (Figure 4A and 4B).
T8688	18428-18438	JJ	denotes	Consistent
T8689	18547-18550	VBD	denotes	had
T8690	18439-18443	IN	denotes	with
T8691	18444-18447	PRP$	denotes	our
T8692	18455-18463	NN	denotes	analysis
T8693	18448-18454	JJ	denotes	visual
T8694	18464-18466	IN	denotes	of
T8695	18467-18470	DT	denotes	the
T8696	18484-18492	NNS	denotes	sections
T8697	18471-18483	JJ	denotes	histological
T8698	18492-18494	,	denotes	,
T8699	18494-18498	NN	denotes	line
T8700	18511-18515	NNS	denotes	mice
T8701	18499-18502	CD	denotes	107
T8702	18503-18506	NN	denotes	tTA
T8703	18507-18510	NN	denotes	APP
T8704	18506-18507	HYPH	denotes	/
T8705	18516-18523	VBN	denotes	treated
T8706	18524-18528	IN	denotes	with
T8707	18529-18532	NN	denotes	dox
T8708	18533-18536	IN	denotes	for
T8709	18537-18538	CD	denotes	3
T8710	18544-18546	NN	denotes	mo
T8711	18539-18541	CC	denotes	or
T8712	18542-18543	CD	denotes	6
T8713	18551-18554	DT	denotes	the
T8714	18560-18566	NN	denotes	amount
T8715	18555-18559	JJ	denotes	same
T8716	18567-18569	IN	denotes	of
T8717	18570-18580	VBN	denotes	aggregated
T8718	18581-18583	NN	denotes	Aβ
T8719	18584-18586	IN	denotes	as
T8720	18587-18591	WRB	denotes	when
T8721	18597-18604	VBD	denotes	started
T8722	18592-18596	PRP	denotes	they
T8723	18605-18614	NN	denotes	treatment
T8724	18615-18617	IN	denotes	at
T8725	18618-18619	CD	denotes	6
T8726	18620-18622	NN	denotes	mo
T8727	18623-18625	IN	denotes	of
T8728	18626-18629	NN	denotes	age
T8729	18630-18631	-LRB-	denotes	(
T8730	18638-18640	NN	denotes	4A
T8731	18631-18637	NN	denotes	Figure
T8732	18641-18644	CC	denotes	and
T8733	18645-18647	NN	denotes	4B
T8734	18647-18648	-RRB-	denotes	)
T8735	18648-18649	.	denotes	.
T8736	18649-18758	sentence	denotes	In contrast, untreated 9-mo-old mice had almost twice as much aggregated Aβ as either of the treated groups.
T8737	18650-18652	IN	denotes	In
T8738	18687-18690	VBD	denotes	had
T8739	18653-18661	NN	denotes	contrast
T8740	18661-18663	,	denotes	,
T8741	18663-18672	JJ	denotes	untreated
T8742	18682-18686	NNS	denotes	mice
T8743	18673-18674	CD	denotes	9
T8744	18675-18677	NN	denotes	mo
T8745	18674-18675	HYPH	denotes	-
T8746	18678-18681	JJ	denotes	old
T8747	18677-18678	HYPH	denotes	-
T8748	18691-18697	RB	denotes	almost
T8749	18698-18703	RB	denotes	twice
T8750	18707-18711	JJ	denotes	much
T8751	18704-18706	RB	denotes	as
T8752	18723-18725	NN	denotes	Aβ
T8753	18712-18722	VBN	denotes	aggregated
T8754	18726-18728	IN	denotes	as
T8755	18729-18735	DT	denotes	either
T8756	18736-18738	IN	denotes	of
T8757	18739-18742	DT	denotes	the
T8758	18751-18757	NNS	denotes	groups
T8759	18743-18750	VBN	denotes	treated
T8760	18757-18758	.	denotes	.
T8761	18758-18985	sentence	denotes	Filter trap analysis of line 18 tTA/APP mice yielded similar results: the increase in aggregated Aβ observed in untreated animals between 6 and 9 mo of age was completely arrested by transgene suppression (Figure S5C and S5D).
T8762	18759-18765	NN	denotes	Filter
T8763	18771-18779	NN	denotes	analysis
T8764	18766-18770	NN	denotes	trap
T8765	18804-18811	VBD	denotes	yielded
T8766	18780-18782	IN	denotes	of
T8767	18783-18787	NN	denotes	line
T8768	18799-18803	NNS	denotes	mice
T8769	18788-18790	CD	denotes	18
T8770	18791-18794	NN	denotes	tTA
T8771	18795-18798	NN	denotes	APP
T8772	18794-18795	HYPH	denotes	/
T8773	18930-18938	VBN	denotes	arrested
T8774	18812-18819	JJ	denotes	similar
T8775	18820-18827	NNS	denotes	results
T8776	18827-18829	:	denotes	:
T8777	18829-18832	DT	denotes	the
T8778	18833-18841	NN	denotes	increase
T8779	18842-18844	IN	denotes	in
T8780	18845-18855	VBN	denotes	aggregated
T8781	18856-18858	NN	denotes	Aβ
T8782	18859-18867	VBN	denotes	observed
T8783	18868-18870	IN	denotes	in
T8784	18871-18880	JJ	denotes	untreated
T8785	18881-18888	NNS	denotes	animals
T8786	18889-18896	IN	denotes	between
T8787	18897-18898	CD	denotes	6
T8788	18905-18907	NN	denotes	mo
T8789	18899-18902	CC	denotes	and
T8790	18903-18904	CD	denotes	9
T8791	18908-18910	IN	denotes	of
T8792	18911-18914	NN	denotes	age
T8793	18915-18918	VBD	denotes	was
T8794	18919-18929	RB	denotes	completely
T8795	18939-18941	IN	denotes	by
T8796	18942-18951	NN	denotes	transgene
T8797	18952-18963	NN	denotes	suppression
T8798	18964-18965	-LRB-	denotes	(
T8799	18972-18975	NN	denotes	S5C
T8800	18965-18971	NN	denotes	Figure
T8801	18976-18979	CC	denotes	and
T8802	18980-18983	NN	denotes	S5D
T8803	18983-18984	-RRB-	denotes	)
T8804	18984-18985	.	denotes	.
T8805	18985-19176	sentence	denotes	We next used ELISA to measure total Aβ in the brains of each group to determine whether any change in the amount or solubility of peptide occurred while APPswe/ind expression was suppressed.
T8806	18986-18988	PRP	denotes	We
T8807	18994-18998	VBD	denotes	used
T8808	18989-18993	RB	denotes	next
T8809	18999-19004	NN	denotes	ELISA
T8810	19005-19007	TO	denotes	to
T8811	19008-19015	VB	denotes	measure
T8812	19016-19021	JJ	denotes	total
T8813	19022-19024	NN	denotes	Aβ
T8814	19025-19027	IN	denotes	in
T8815	19028-19031	DT	denotes	the
T8816	19032-19038	NNS	denotes	brains
T8817	19039-19041	IN	denotes	of
T8818	19042-19046	DT	denotes	each
T8819	19047-19052	NN	denotes	group
T8820	19053-19055	TO	denotes	to
T8821	19056-19065	VB	denotes	determine
T8822	19066-19073	IN	denotes	whether
T8823	19124-19132	VBD	denotes	occurred
T8824	19074-19077	DT	denotes	any
T8825	19078-19084	NN	denotes	change
T8826	19085-19087	IN	denotes	in
T8827	19088-19091	DT	denotes	the
T8828	19092-19098	NN	denotes	amount
T8829	19099-19101	CC	denotes	or
T8830	19102-19112	NN	denotes	solubility
T8831	19113-19115	IN	denotes	of
T8832	19116-19123	NN	denotes	peptide
T8833	19133-19138	IN	denotes	while
T8834	19165-19175	VBN	denotes	suppressed
T8835	19139-19145	NN	denotes	APPswe
T8836	19146-19149	NN	denotes	ind
T8837	19145-19146	HYPH	denotes	/
T8838	19150-19160	NN	denotes	expression
T8839	19161-19164	VBD	denotes	was
T8840	19175-19176	.	denotes	.
T8841	19176-19369	sentence	denotes	Cortical homogenates were sequentially extracted to separate peptide into PBS-, SDS-, and FA-soluble fractions, then transgene-derived Aβ40 and Aβ42 were measured by human-specific ELISA [23].
T8842	19177-19185	JJ	denotes	Cortical
T8843	19186-19197	NNS	denotes	homogenates
T8844	19216-19225	VBN	denotes	extracted
T8845	19198-19202	VBD	denotes	were
T8846	19203-19215	RB	denotes	sequentially
T8847	19331-19339	VBN	denotes	measured
T8848	19226-19228	TO	denotes	to
T8849	19229-19237	VB	denotes	separate
T8850	19238-19245	NN	denotes	peptide
T8851	19246-19250	IN	denotes	into
T8852	19251-19254	NN	denotes	PBS
T8853	19270-19277	JJ	denotes	soluble
T8854	19254-19255	HYPH	denotes	-
T8855	19255-19257	,	denotes	,
T8856	19257-19260	NN	denotes	SDS
T8857	19260-19261	HYPH	denotes	-
T8858	19261-19263	,	denotes	,
T8859	19263-19266	CC	denotes	and
T8860	19267-19269	NN	denotes	FA
T8861	19269-19270	HYPH	denotes	-
T8862	19278-19287	NNS	denotes	fractions
T8863	19287-19289	,	denotes	,
T8864	19289-19293	RB	denotes	then
T8865	19294-19303	NN	denotes	transgene
T8866	19304-19311	VBN	denotes	derived
T8867	19303-19304	HYPH	denotes	-
T8868	19312-19316	NN	denotes	Aβ40
T8869	19317-19320	CC	denotes	and
T8870	19321-19325	NN	denotes	Aβ42
T8871	19326-19330	VBD	denotes	were
T8872	19340-19342	IN	denotes	by
T8873	19343-19348	JJ	denotes	human
T8874	19349-19357	JJ	denotes	specific
T8875	19348-19349	HYPH	denotes	-
T8876	19358-19363	NN	denotes	ELISA
T8877	19364-19365	-LRB-	denotes	[
T8878	19365-19367	CD	denotes	23
T8879	19367-19368	-RRB-	denotes	]
T8880	19368-19369	.	denotes	.
T8881	19369-19521	sentence	denotes	In all animals harboring amyloid deposits, we found that the vast majority of Aβ (>99%) was extracted into the SDS and FA fractions (Figure 5A and 5B).
T8882	19370-19372	IN	denotes	In
T8883	19416-19421	VBD	denotes	found
T8884	19373-19376	DT	denotes	all
T8885	19377-19384	NNS	denotes	animals
T8886	19385-19394	VBG	denotes	harboring
T8887	19395-19402	NN	denotes	amyloid
T8888	19403-19411	NNS	denotes	deposits
T8889	19411-19413	,	denotes	,
T8890	19413-19415	PRP	denotes	we
T8891	19422-19426	IN	denotes	that
T8892	19462-19471	VBN	denotes	extracted
T8893	19427-19430	DT	denotes	the
T8894	19436-19444	NN	denotes	majority
T8895	19431-19435	JJ	denotes	vast
T8896	19445-19447	IN	denotes	of
T8897	19448-19450	NN	denotes	Aβ
T8898	19451-19452	-LRB-	denotes	(
T8899	19455-19456	NN	denotes	%
T8900	19452-19453	SYM	denotes	>
T8901	19453-19455	CD	denotes	99
T8902	19456-19457	-RRB-	denotes	)
T8903	19458-19461	VBD	denotes	was
T8904	19472-19476	IN	denotes	into
T8905	19477-19480	DT	denotes	the
T8906	19492-19501	NNS	denotes	fractions
T8907	19481-19484	NN	denotes	SDS
T8908	19485-19488	CC	denotes	and
T8909	19489-19491	NN	denotes	FA
T8910	19502-19503	-LRB-	denotes	(
T8911	19510-19512	NN	denotes	5A
T8912	19503-19509	NN	denotes	Figure
T8913	19513-19516	CC	denotes	and
T8914	19517-19519	NN	denotes	5B
T8915	19519-19520	-RRB-	denotes	)
T8916	19520-19521	.	denotes	.
T8917	19521-19742	sentence	denotes	Consistent with the filter trap results presented above, there were no significant differences in SDS- or FA-soluble Aβ between the 6 mo untreated cohort and either the 6 mo + 3 mo treated or 6 mo + 6 mo treated cohorts.
T8918	19522-19532	JJ	denotes	Consistent
T8919	19585-19589	VBD	denotes	were
T8920	19533-19537	IN	denotes	with
T8921	19538-19541	DT	denotes	the
T8922	19554-19561	NNS	denotes	results
T8923	19542-19548	NN	denotes	filter
T8924	19549-19553	NN	denotes	trap
T8925	19562-19571	VBN	denotes	presented
T8926	19572-19577	RB	denotes	above
T8927	19577-19579	,	denotes	,
T8928	19579-19584	EX	denotes	there
T8929	19590-19592	DT	denotes	no
T8930	19605-19616	NNS	denotes	differences
T8931	19593-19604	JJ	denotes	significant
T8932	19617-19619	IN	denotes	in
T8933	19620-19623	NN	denotes	SDS
T8934	19631-19638	JJ	denotes	soluble
T8935	19623-19624	HYPH	denotes	-
T8936	19625-19627	CC	denotes	or
T8937	19628-19630	NN	denotes	FA
T8938	19630-19631	HYPH	denotes	-
T8939	19639-19641	NN	denotes	Aβ
T8940	19642-19649	IN	denotes	between
T8941	19650-19653	DT	denotes	the
T8942	19669-19675	NN	denotes	cohort
T8943	19654-19655	CD	denotes	6
T8944	19656-19658	NN	denotes	mo
T8945	19659-19668	JJ	denotes	untreated
T8946	19676-19679	CC	denotes	and
T8947	19680-19686	CC	denotes	either
T8948	19734-19741	NNS	denotes	cohorts
T8949	19687-19690	DT	denotes	the
T8950	19691-19692	CD	denotes	6
T8951	19693-19695	NN	denotes	mo
T8952	19696-19697	SYM	denotes	+
T8953	19698-19699	CD	denotes	3
T8954	19700-19702	NN	denotes	mo
T8955	19703-19710	VBN	denotes	treated
T8956	19711-19713	CC	denotes	or
T8957	19714-19715	CD	denotes	6
T8958	19716-19718	NN	denotes	mo
T8959	19719-19720	SYM	denotes	+
T8960	19721-19722	CD	denotes	6
T8961	19723-19725	NN	denotes	mo
T8962	19726-19733	VBN	denotes	treated
T8963	19741-19742	.	denotes	.
T8964	19742-19899	sentence	denotes	However, brains of both 6 mo + 3 mo and 6 mo + 6 mo treated cohorts contained roughly twice as much PBS-soluble Aβ40 as untreated 6-mo-old mice (Figure 5C).
T8965	19743-19750	RB	denotes	However
T8966	19811-19820	VBD	denotes	contained
T8967	19750-19752	,	denotes	,
T8968	19752-19758	NNS	denotes	brains
T8969	19759-19761	IN	denotes	of
T8970	19762-19766	CC	denotes	both
T8971	19769-19771	NN	denotes	mo
T8972	19767-19768	CD	denotes	6
T8973	19795-19802	VBN	denotes	treated
T8974	19772-19773	SYM	denotes	+
T8975	19774-19775	CD	denotes	3
T8976	19776-19778	NN	denotes	mo
T8977	19779-19782	CC	denotes	and
T8978	19783-19784	CD	denotes	6
T8979	19785-19787	NN	denotes	mo
T8980	19788-19789	SYM	denotes	+
T8981	19790-19791	CD	denotes	6
T8982	19792-19794	NN	denotes	mo
T8983	19803-19810	NNS	denotes	cohorts
T8984	19821-19828	RB	denotes	roughly
T8985	19829-19834	RB	denotes	twice
T8986	19838-19842	JJ	denotes	much
T8987	19835-19837	RB	denotes	as
T8988	19855-19859	NN	denotes	Aβ40
T8989	19843-19846	NN	denotes	PBS
T8990	19846-19847	HYPH	denotes	-
T8991	19847-19854	JJ	denotes	soluble
T8992	19860-19862	IN	denotes	as
T8993	19863-19872	JJ	denotes	untreated
T8994	19882-19886	NNS	denotes	mice
T8995	19873-19874	CD	denotes	6
T8996	19875-19877	NN	denotes	mo
T8997	19874-19875	HYPH	denotes	-
T8998	19878-19881	JJ	denotes	old
T8999	19877-19878	HYPH	denotes	-
T9000	19887-19888	-LRB-	denotes	(
T9001	19895-19897	NN	denotes	5C
T9002	19888-19894	NN	denotes	Figure
T9003	19897-19898	-RRB-	denotes	)
T9004	19898-19899	.	denotes	.
T9005	19899-19982	sentence	denotes	Levels of Aβ42 showed a similar trend, but did not reach statistical significance.
T9006	19900-19906	NNS	denotes	Levels
T9007	19915-19921	VBD	denotes	showed
T9008	19907-19909	IN	denotes	of
T9009	19910-19914	NN	denotes	Aβ42
T9010	19922-19923	DT	denotes	a
T9011	19932-19937	NN	denotes	trend
T9012	19924-19931	JJ	denotes	similar
T9013	19937-19939	,	denotes	,
T9014	19939-19942	CC	denotes	but
T9015	19943-19946	VBD	denotes	did
T9016	19951-19956	VB	denotes	reach
T9017	19947-19950	RB	denotes	not
T9018	19957-19968	JJ	denotes	statistical
T9019	19969-19981	NN	denotes	significance
T9020	19981-19982	.	denotes	.
T9021	19982-20299	sentence	denotes	In fact, levels of PBS-soluble Aβ40 and Aβ42 in the 6 mo + 3 mo and 6 mo + 6 mo treated cohorts were most similar to that of the 9 mo untreated cohort, suggesting that age, as opposed to synthetic rate (which would be negligible in the treated animals), may determine the fraction of PBS-soluble Aβ in these animals.
T9022	19983-19985	IN	denotes	In
T9023	20079-20083	VBD	denotes	were
T9024	19986-19990	NN	denotes	fact
T9025	19990-19992	,	denotes	,
T9026	19992-19998	NNS	denotes	levels
T9027	19999-20001	IN	denotes	of
T9028	20002-20005	NN	denotes	PBS
T9029	20006-20013	JJ	denotes	soluble
T9030	20005-20006	HYPH	denotes	-
T9031	20014-20018	NN	denotes	Aβ40
T9032	20019-20022	CC	denotes	and
T9033	20023-20027	NN	denotes	Aβ42
T9034	20028-20030	IN	denotes	in
T9035	20031-20034	DT	denotes	the
T9036	20071-20078	NNS	denotes	cohorts
T9037	20035-20036	CD	denotes	6
T9038	20037-20039	NN	denotes	mo
T9039	20040-20041	SYM	denotes	+
T9040	20042-20043	CD	denotes	3
T9041	20044-20046	NN	denotes	mo
T9042	20047-20050	CC	denotes	and
T9043	20051-20052	CD	denotes	6
T9044	20053-20055	NN	denotes	mo
T9045	20056-20057	SYM	denotes	+
T9046	20058-20059	CD	denotes	6
T9047	20060-20062	NN	denotes	mo
T9048	20063-20070	VBN	denotes	treated
T9049	20084-20088	RBS	denotes	most
T9050	20089-20096	JJ	denotes	similar
T9051	20097-20099	IN	denotes	to
T9052	20100-20104	DT	denotes	that
T9053	20105-20107	IN	denotes	of
T9054	20108-20111	DT	denotes	the
T9055	20127-20133	NN	denotes	cohort
T9056	20112-20113	CD	denotes	9
T9057	20114-20116	NN	denotes	mo
T9058	20117-20126	JJ	denotes	untreated
T9059	20133-20135	,	denotes	,
T9060	20135-20145	VBG	denotes	suggesting
T9061	20146-20150	IN	denotes	that
T9062	20241-20250	VB	denotes	determine
T9063	20151-20154	NN	denotes	age
T9064	20154-20156	,	denotes	,
T9065	20156-20158	IN	denotes	as
T9066	20159-20166	VBN	denotes	opposed
T9067	20167-20169	IN	denotes	to
T9068	20170-20179	JJ	denotes	synthetic
T9069	20180-20184	NN	denotes	rate
T9070	20185-20186	-LRB-	denotes	(
T9071	20186-20191	WDT	denotes	which
T9072	20198-20200	VB	denotes	be
T9073	20192-20197	MD	denotes	would
T9074	20201-20211	JJ	denotes	negligible
T9075	20212-20214	IN	denotes	in
T9076	20215-20218	DT	denotes	the
T9077	20227-20234	NNS	denotes	animals
T9078	20219-20226	VBN	denotes	treated
T9079	20234-20235	-RRB-	denotes	)
T9080	20235-20237	,	denotes	,
T9081	20237-20240	MD	denotes	may
T9082	20251-20254	DT	denotes	the
T9083	20255-20263	NN	denotes	fraction
T9084	20264-20266	IN	denotes	of
T9085	20267-20270	NN	denotes	PBS
T9086	20271-20278	JJ	denotes	soluble
T9087	20270-20271	HYPH	denotes	-
T9088	20279-20281	NN	denotes	Aβ
T9089	20282-20284	IN	denotes	in
T9090	20285-20290	DT	denotes	these
T9091	20291-20298	NNS	denotes	animals
T9092	20298-20299	.	denotes	.
T9093	20299-22627	sentence	denotes	Figure 5 Aβ ELISA Confirms Arrest of Progression without Clearance of Peptide in Mice with Preexisting Aggregates Aβ levels in untreated 6- and 9-mo-old tTA/APP line 107 mice (shown in Figure 4) were compared to those in 9- and 12-mo-old animals treated with dox from the age of 6 mo. Single transgenic APP samples were included as negative controls. Cortical homogenates were fractionated by sequential multi-step extraction with PBS, 2% SDS, and 70% FA followed by human-specific Aβ ELISA to measure transgene-derived peptide in each fraction. Aβ40 is shown in white, Aβ42 in black. (A and B) Most Aβ in the brains of plaque-bearing mice is extracted into the FA and SDS fractions. Consistent with amyloid burden (Figures 4 and Figure S3), SDS- and FA-extracted Aβ levels in untreated 9-mo-old mice were significantly higher than in untreated 6-mo-old mice (Tukey post-hoc test applied to significant effect of group ANOVA for SDS and FA fractions F 3,18 = 4.72–12.92, p < 0.02). In contrast, 3 or 6 mo of transgene suppression held Aβ at levels equivalent to those harbored when treatment was started (p > 0.2 compared to 6 mo untreated mice, Tukey post-hoc test). , p < 0.05; , p < 0.005; **, p < 0.001 versus 9-mo-old untreated mice, Tukey post-hoc test. Significance for APP versus 9-mo-old untreated mice is based on Student's t-test. (C) The PBS fraction represents less than 0.1% of total Aβ (note the change in y-axis from [A] and [B]), but only here do Aβ levels in the dox-treated mice differ from those in younger untreated mice. Although both peptides appear elevated in the treated groups compared to the untreated 6-mo-old mice, only Aβ40 reaches statistical significance (p < 0.05, Tukey post-hoc test applied to significant effect of group ANOVA for Aβ40 F 3,18 = 4.60, p < 0.02). A similar trend was seen for Aβ42, where ANOVA yielded a significant effect of group for PBS-soluble Aβ42 (F 3,18 = 3.75, p < 0.03), however this was due only to differences between the untreated 6- and 9-mo-old groups. •, p < 0.05 versus 6-mo-old untreated mice, Tukey post-hoc test; •••, p < 0.001 versus 6-mo-old untreated mice, Student's t-test. We also assessed neuritic and glial pathology surrounding the plaques to determine whether there were any changes in nearby tissue following long-term transgene suppression.
T9094	22454-22456	PRP	denotes	We
T9095	22462-22470	VBD	denotes	assessed
T9096	22457-22461	RB	denotes	also
T9097	22471-22479	JJ	denotes	neuritic
T9098	22490-22499	NN	denotes	pathology
T9099	22480-22483	CC	denotes	and
T9100	22484-22489	JJ	denotes	glial
T9101	22500-22511	VBG	denotes	surrounding
T9102	22512-22515	DT	denotes	the
T9103	22516-22523	NNS	denotes	plaques
T9104	22524-22526	TO	denotes	to
T9105	22527-22536	VB	denotes	determine
T9106	22537-22544	IN	denotes	whether
T9107	22551-22555	VBD	denotes	were
T9108	22545-22550	EX	denotes	there
T9109	22556-22559	DT	denotes	any
T9110	22560-22567	NNS	denotes	changes
T9111	22568-22570	IN	denotes	in
T9112	22571-22577	JJ	denotes	nearby
T9113	22578-22584	NN	denotes	tissue
T9114	22585-22594	VBG	denotes	following
T9115	22595-22599	JJ	denotes	long
T9116	22600-22604	NN	denotes	term
T9117	22599-22600	HYPH	denotes	-
T9118	22615-22626	NN	denotes	suppression
T9119	22605-22614	NN	denotes	transgene
T9120	22626-22627	.	denotes	.
T9121	22627-22743	sentence	denotes	Both Hirano silver stain and ubiquitin immunostaining showed neuritic pathology in all treatment groups (Figure 6).
T9122	22628-22632	CC	denotes	Both
T9123	22647-22652	NN	denotes	stain
T9124	22633-22639	NNP	denotes	Hirano
T9125	22640-22646	NN	denotes	silver
T9126	22682-22688	VBD	denotes	showed
T9127	22653-22656	CC	denotes	and
T9128	22657-22666	NN	denotes	ubiquitin
T9129	22667-22681	NN	denotes	immunostaining
T9130	22689-22697	JJ	denotes	neuritic
T9131	22698-22707	NN	denotes	pathology
T9132	22708-22710	IN	denotes	in
T9133	22711-22714	DT	denotes	all
T9134	22725-22731	NNS	denotes	groups
T9135	22715-22724	NN	denotes	treatment
T9136	22732-22733	-LRB-	denotes	(
T9137	22733-22739	NN	denotes	Figure
T9138	22740-22741	CD	denotes	6
T9139	22741-22742	-RRB-	denotes	)
T9140	22742-22743	.	denotes	.
T9141	22743-22849	sentence	denotes	Similarly, activated astrocytes immunostained for GFAP were found near plaques in all animals (Figure 6).
T9142	22744-22753	RB	denotes	Similarly
T9143	22804-22809	VBN	denotes	found
T9144	22753-22755	,	denotes	,
T9145	22755-22764	VBN	denotes	activated
T9146	22765-22775	NNS	denotes	astrocytes
T9147	22776-22789	VBN	denotes	immunostained
T9148	22790-22793	IN	denotes	for
T9149	22794-22798	NN	denotes	GFAP
T9150	22799-22803	VBD	denotes	were
T9151	22810-22814	IN	denotes	near
T9152	22815-22822	NNS	denotes	plaques
T9153	22823-22825	IN	denotes	in
T9154	22826-22829	DT	denotes	all
T9155	22830-22837	NNS	denotes	animals
T9156	22838-22839	-LRB-	denotes	(
T9157	22839-22845	NN	denotes	Figure
T9158	22846-22847	CD	denotes	6
T9159	22847-22848	-RRB-	denotes	)
T9160	22848-22849	.	denotes	.
T9161	22849-22924	sentence	denotes	Neuritic and glial pathology were more severe in the older untreated mice.
T9162	22850-22858	JJ	denotes	Neuritic
T9163	22869-22878	NN	denotes	pathology
T9164	22859-22862	CC	denotes	and
T9165	22863-22868	JJ	denotes	glial
T9166	22879-22883	VBD	denotes	were
T9167	22884-22888	RBR	denotes	more
T9168	22889-22895	JJ	denotes	severe
T9169	22896-22898	IN	denotes	in
T9170	22899-22902	DT	denotes	the
T9171	22919-22923	NNS	denotes	mice
T9172	22903-22908	JJR	denotes	older
T9173	22909-22918	JJ	denotes	untreated
T9174	22923-22924	.	denotes	.
T9175	22924-23119	sentence	denotes	In contrast, transgene suppression prevented the growth of individual deposits apparent in untreated mice, and limited the surrounding pathology to what was already present when treatment began.
T9176	22925-22927	IN	denotes	In
T9177	22960-22969	VBD	denotes	prevented
T9178	22928-22936	NN	denotes	contrast
T9179	22936-22938	,	denotes	,
T9180	22938-22947	NN	denotes	transgene
T9181	22948-22959	NN	denotes	suppression
T9182	22970-22973	DT	denotes	the
T9183	22974-22980	NN	denotes	growth
T9184	22981-22983	IN	denotes	of
T9185	22984-22994	JJ	denotes	individual
T9186	22995-23003	NNS	denotes	deposits
T9187	23004-23012	JJ	denotes	apparent
T9188	23013-23015	IN	denotes	in
T9189	23016-23025	JJ	denotes	untreated
T9190	23026-23030	NNS	denotes	mice
T9191	23030-23032	,	denotes	,
T9192	23032-23035	CC	denotes	and
T9193	23036-23043	VBD	denotes	limited
T9194	23044-23047	DT	denotes	the
T9195	23060-23069	NN	denotes	pathology
T9196	23048-23059	VBG	denotes	surrounding
T9197	23070-23072	IN	denotes	to
T9198	23073-23077	WP	denotes	what
T9199	23078-23081	VBD	denotes	was
T9200	23082-23089	RB	denotes	already
T9201	23090-23097	JJ	denotes	present
T9202	23098-23102	WRB	denotes	when
T9203	23113-23118	VBD	denotes	began
T9204	23103-23112	NN	denotes	treatment
T9205	23118-23119	.	denotes	.
T9206	23119-23882	sentence	denotes	Figure 6 Neuritic and Glial Pathology Are Unchanged following Transgene Suppression Dystrophic neurites and activated astrocytes surround most compact plaques in tet-off APP mice (line 107). Dark-stained, ubiquitin-filled neurites and reactive astrocytes form a halo around cored, fibrillar deposits by 6 mo of age that worsens with time in untreated mice. Both plaque-associated pathologies are arrested, although not reversed, by transgene suppression. Hirano silver stain (top row); GFAP immunohistochemistry (middle row); ubiquitin immunohistochemistry (bottom row). An obvious question we sought to address was whether the deposition of Aβ diminished cognitive ability in untreated mice, and what might happen to cognition when the process was interrupted.
T9207	23692-23694	DT	denotes	An
T9208	23703-23711	NN	denotes	question
T9209	23695-23702	JJ	denotes	obvious
T9210	23733-23736	VBD	denotes	was
T9211	23712-23714	PRP	denotes	we
T9212	23715-23721	VBD	denotes	sought
T9213	23722-23724	TO	denotes	to
T9214	23725-23732	VB	denotes	address
T9215	23737-23744	IN	denotes	whether
T9216	23766-23776	VBD	denotes	diminished
T9217	23745-23748	DT	denotes	the
T9218	23749-23759	NN	denotes	deposition
T9219	23760-23762	IN	denotes	of
T9220	23763-23765	NN	denotes	Aβ
T9221	23777-23786	JJ	denotes	cognitive
T9222	23787-23794	NN	denotes	ability
T9223	23795-23797	IN	denotes	in
T9224	23798-23807	JJ	denotes	untreated
T9225	23808-23812	NNS	denotes	mice
T9226	23812-23814	,	denotes	,
T9227	23814-23817	CC	denotes	and
T9228	23818-23822	WP	denotes	what
T9229	23829-23835	VB	denotes	happen
T9230	23823-23828	MD	denotes	might
T9231	23836-23838	IN	denotes	to
T9232	23839-23848	NN	denotes	cognition
T9233	23849-23853	WRB	denotes	when
T9234	23870-23881	VBN	denotes	interrupted
T9235	23854-23857	DT	denotes	the
T9236	23858-23865	NN	denotes	process
T9237	23866-23869	VBD	denotes	was
T9238	23881-23882	.	denotes	.
T9239	23882-24018	sentence	denotes	Unfortunately, efforts to characterize cognitive behavior were compromised by severe hyperactivity in untreated double transgenic mice.
T9240	23883-23896	RB	denotes	Unfortunately
T9241	23946-23957	VBN	denotes	compromised
T9242	23896-23898	,	denotes	,
T9243	23898-23905	NNS	denotes	efforts
T9244	23906-23908	TO	denotes	to
T9245	23909-23921	VB	denotes	characterize
T9246	23922-23931	JJ	denotes	cognitive
T9247	23932-23940	NN	denotes	behavior
T9248	23941-23945	VBD	denotes	were
T9249	23958-23960	IN	denotes	by
T9250	23961-23967	JJ	denotes	severe
T9251	23968-23981	NN	denotes	hyperactivity
T9252	23982-23984	IN	denotes	in
T9253	23985-23994	JJ	denotes	untreated
T9254	24013-24017	NNS	denotes	mice
T9255	23995-24001	JJ	denotes	double
T9256	24002-24012	JJ	denotes	transgenic
T9257	24017-24018	.	denotes	.
T9258	24018-24203	sentence	denotes	The tTA/APP animals were often seen running in circles around the perimeter of their cages, and a similar swimming pattern was noted when the mice were tested in the Morris water maze.
T9259	24019-24022	DT	denotes	The
T9260	24031-24038	NNS	denotes	animals
T9261	24023-24026	NN	denotes	tTA
T9262	24027-24030	NN	denotes	APP
T9263	24026-24027	HYPH	denotes	/
T9264	24050-24054	VBN	denotes	seen
T9265	24039-24043	VBD	denotes	were
T9266	24044-24049	RB	denotes	often
T9267	24055-24062	VBG	denotes	running
T9268	24063-24065	IN	denotes	in
T9269	24066-24073	NNS	denotes	circles
T9270	24074-24080	IN	denotes	around
T9271	24081-24084	DT	denotes	the
T9272	24085-24094	NN	denotes	perimeter
T9273	24095-24097	IN	denotes	of
T9274	24098-24103	PRP$	denotes	their
T9275	24104-24109	NNS	denotes	cages
T9276	24109-24111	,	denotes	,
T9277	24111-24114	CC	denotes	and
T9278	24115-24116	DT	denotes	a
T9279	24134-24141	NN	denotes	pattern
T9280	24117-24124	JJ	denotes	similar
T9281	24125-24133	NN	denotes	swimming
T9282	24146-24151	VBN	denotes	noted
T9283	24142-24145	VBD	denotes	was
T9284	24152-24156	WRB	denotes	when
T9285	24171-24177	VBN	denotes	tested
T9286	24157-24160	DT	denotes	the
T9287	24161-24165	NNS	denotes	mice
T9288	24166-24170	VBD	denotes	were
T9289	24178-24180	IN	denotes	in
T9290	24181-24184	DT	denotes	the
T9291	24198-24202	NN	denotes	maze
T9292	24185-24191	NNP	denotes	Morris
T9293	24192-24197	NN	denotes	water
T9294	24202-24203	.	denotes	.
T9295	24203-24311	sentence	denotes	In the radial water maze, repetitive swim patterns were noted with no evidence of choice-motivated actions.
T9296	24204-24206	IN	denotes	In
T9297	24260-24265	VBN	denotes	noted
T9298	24207-24210	DT	denotes	the
T9299	24224-24228	NN	denotes	maze
T9300	24211-24217	JJ	denotes	radial
T9301	24218-24223	NN	denotes	water
T9302	24228-24230	,	denotes	,
T9303	24230-24240	JJ	denotes	repetitive
T9304	24246-24254	NNS	denotes	patterns
T9305	24241-24245	NN	denotes	swim
T9306	24255-24259	VBD	denotes	were
T9307	24266-24270	IN	denotes	with
T9308	24271-24273	DT	denotes	no
T9309	24274-24282	NN	denotes	evidence
T9310	24283-24285	IN	denotes	of
T9311	24286-24292	NN	denotes	choice
T9312	24293-24302	VBN	denotes	motivated
T9313	24292-24293	HYPH	denotes	-
T9314	24303-24310	NNS	denotes	actions
T9315	24310-24311	.	denotes	.
T9316	24311-24499	sentence	denotes	Other studies have dealt with similar problems by excluding animals that do not show adequate attention to the task, retaining only those mice that meet certain performance criteria [30].
T9317	24312-24317	JJ	denotes	Other
T9318	24318-24325	NNS	denotes	studies
T9319	24331-24336	VBN	denotes	dealt
T9320	24326-24330	VBP	denotes	have
T9321	24337-24341	IN	denotes	with
T9322	24342-24349	JJ	denotes	similar
T9323	24350-24358	NNS	denotes	problems
T9324	24359-24361	IN	denotes	by
T9325	24362-24371	VBG	denotes	excluding
T9326	24372-24379	NNS	denotes	animals
T9327	24380-24384	WDT	denotes	that
T9328	24392-24396	VB	denotes	show
T9329	24385-24387	VBP	denotes	do
T9330	24388-24391	RB	denotes	not
T9331	24397-24405	JJ	denotes	adequate
T9332	24406-24415	NN	denotes	attention
T9333	24416-24418	IN	denotes	to
T9334	24419-24422	DT	denotes	the
T9335	24423-24427	NN	denotes	task
T9336	24427-24429	,	denotes	,
T9337	24429-24438	VBG	denotes	retaining
T9338	24439-24443	RB	denotes	only
T9339	24450-24454	NNS	denotes	mice
T9340	24444-24449	DT	denotes	those
T9341	24455-24459	WDT	denotes	that
T9342	24460-24464	VBP	denotes	meet
T9343	24465-24472	JJ	denotes	certain
T9344	24485-24493	NNS	denotes	criteria
T9345	24473-24484	NN	denotes	performance
T9346	24494-24495	-LRB-	denotes	[
T9347	24495-24497	CD	denotes	30
T9348	24497-24498	-RRB-	denotes	]
T9349	24498-24499	.	denotes	.
T9350	24499-24600	sentence	denotes	In our case, the penetrance of hyperactivity was close to 100%, leaving us with no testable animals.
T9351	24500-24502	IN	denotes	In
T9352	24545-24548	VBD	denotes	was
T9353	24503-24506	PRP$	denotes	our
T9354	24507-24511	NN	denotes	case
T9355	24511-24513	,	denotes	,
T9356	24513-24516	DT	denotes	the
T9357	24517-24527	NN	denotes	penetrance
T9358	24528-24530	IN	denotes	of
T9359	24531-24544	NN	denotes	hyperactivity
T9360	24549-24554	JJ	denotes	close
T9361	24558-24561	CD	denotes	100
T9362	24555-24557	IN	denotes	to
T9363	24561-24562	NN	denotes	%
T9364	24562-24564	,	denotes	,
T9365	24564-24571	VBG	denotes	leaving
T9366	24572-24574	PRP	denotes	us
T9367	24575-24579	IN	denotes	with
T9368	24580-24582	DT	denotes	no
T9369	24592-24599	NNS	denotes	animals
T9370	24583-24591	JJ	denotes	testable
T9371	24599-24600	.	denotes	.
T9372	24600-24757	sentence	denotes	This phenotype has not affected previous lines of APPswe mice we have produced, such as lines E1–2 or C3–3, that express lower levels of transgenic protein.
T9373	24601-24605	DT	denotes	This
T9374	24606-24615	NN	denotes	phenotype
T9375	24624-24632	VBN	denotes	affected
T9376	24616-24619	VBZ	denotes	has
T9377	24620-24623	RB	denotes	not
T9378	24633-24641	JJ	denotes	previous
T9379	24642-24647	NNS	denotes	lines
T9380	24648-24650	IN	denotes	of
T9381	24651-24657	NN	denotes	APPswe
T9382	24658-24662	NNS	denotes	mice
T9383	24663-24665	PRP	denotes	we
T9384	24671-24679	VBN	denotes	produced
T9385	24666-24670	VBP	denotes	have
T9386	24679-24681	,	denotes	,
T9387	24681-24685	JJ	denotes	such
T9388	24686-24688	IN	denotes	as
T9389	24689-24694	NNS	denotes	lines
T9390	24695-24697	NN	denotes	E1
T9391	24697-24698	HYPH	denotes	–
T9392	24698-24699	CD	denotes	2
T9393	24700-24702	CC	denotes	or
T9394	24703-24705	NN	denotes	C3
T9395	24705-24706	HYPH	denotes	–
T9396	24706-24707	CD	denotes	3
T9397	24707-24709	,	denotes	,
T9398	24709-24713	WDT	denotes	that
T9399	24714-24721	VBP	denotes	express
T9400	24722-24727	JJR	denotes	lower
T9401	24728-24734	NNS	denotes	levels
T9402	24735-24737	IN	denotes	of
T9403	24738-24748	JJ	denotes	transgenic
T9404	24749-24756	NN	denotes	protein
T9405	24756-24757	.	denotes	.
T9406	24757-24904	sentence	denotes	Indeed, in past studies where hyperactivity was not a factor, we established a clear relationship between amyloid load and cognitive ability [31].
T9407	24758-24764	RB	denotes	Indeed
T9408	24823-24834	VBD	denotes	established
T9409	24764-24766	,	denotes	,
T9410	24766-24768	IN	denotes	in
T9411	24769-24773	JJ	denotes	past
T9412	24774-24781	NNS	denotes	studies
T9413	24782-24787	WRB	denotes	where
T9414	24802-24805	VBD	denotes	was
T9415	24788-24801	NN	denotes	hyperactivity
T9416	24806-24809	RB	denotes	not
T9417	24810-24811	DT	denotes	a
T9418	24812-24818	NN	denotes	factor
T9419	24818-24820	,	denotes	,
T9420	24820-24822	PRP	denotes	we
T9421	24835-24836	DT	denotes	a
T9422	24843-24855	NN	denotes	relationship
T9423	24837-24842	JJ	denotes	clear
T9424	24856-24863	IN	denotes	between
T9425	24864-24871	NN	denotes	amyloid
T9426	24872-24876	NN	denotes	load
T9427	24877-24880	CC	denotes	and
T9428	24881-24890	JJ	denotes	cognitive
T9429	24891-24898	NN	denotes	ability
T9430	24899-24900	-LRB-	denotes	[
T9431	24900-24902	CD	denotes	31
T9432	24902-24903	-RRB-	denotes	]
T9433	24903-24904	.	denotes	.
T9434	24904-25158	sentence	denotes	However, in the current study, we feel that although the poor performance of the tTA/APP mice in the maze tests could technically be scored as cognitive impairment, the animals' severe hyperactivity made interpretation of the cognitive tasks impossible.
T9435	24905-24912	RB	denotes	However
T9436	24939-24943	VBP	denotes	feel
T9437	24912-24914	,	denotes	,
T9438	24914-24916	IN	denotes	in
T9439	24917-24920	DT	denotes	the
T9440	24929-24934	NN	denotes	study
T9441	24921-24928	JJ	denotes	current
T9442	24934-24936	,	denotes	,
T9443	24936-24938	PRP	denotes	we
T9444	24944-24948	IN	denotes	that
T9445	25104-25108	VBD	denotes	made
T9446	24949-24957	IN	denotes	although
T9447	25038-25044	VBN	denotes	scored
T9448	24958-24961	DT	denotes	the
T9449	24967-24978	NN	denotes	performance
T9450	24962-24966	JJ	denotes	poor
T9451	24979-24981	IN	denotes	of
T9452	24982-24985	DT	denotes	the
T9453	24994-24998	NNS	denotes	mice
T9454	24986-24989	NN	denotes	tTA
T9455	24990-24993	NN	denotes	APP
T9456	24989-24990	HYPH	denotes	/
T9457	24999-25001	IN	denotes	in
T9458	25002-25005	DT	denotes	the
T9459	25011-25016	NNS	denotes	tests
T9460	25006-25010	NN	denotes	maze
T9461	25017-25022	MD	denotes	could
T9462	25023-25034	RB	denotes	technically
T9463	25035-25037	VB	denotes	be
T9464	25045-25047	IN	denotes	as
T9465	25048-25057	JJ	denotes	cognitive
T9466	25058-25068	NN	denotes	impairment
T9467	25068-25070	,	denotes	,
T9468	25070-25073	DT	denotes	the
T9469	25074-25081	NNS	denotes	animals
T9470	25090-25103	NN	denotes	hyperactivity
T9471	25081-25082	POS	denotes	'
T9472	25083-25089	JJ	denotes	severe
T9473	25109-25123	NN	denotes	interpretation
T9474	25147-25157	JJ	denotes	impossible
T9475	25124-25126	IN	denotes	of
T9476	25127-25130	DT	denotes	the
T9477	25141-25146	NNS	denotes	tasks
T9478	25131-25140	JJ	denotes	cognitive
T9479	25157-25158	.	denotes	.
T9480	25158-25447	sentence	denotes	In order to understand the nature and extent of hyperactivity in the tTA/APP mice, we quantified daily activity levels in double transgenic animals along with their single transgenic and nontransgenic siblings using four-beam frames designed to monitor ambulation within an enclosed cage.
T9481	25159-25161	IN	denotes	In
T9482	25245-25255	VBD	denotes	quantified
T9483	25162-25167	NN	denotes	order
T9484	25168-25170	TO	denotes	to
T9485	25171-25181	VB	denotes	understand
T9486	25182-25185	DT	denotes	the
T9487	25186-25192	NN	denotes	nature
T9488	25193-25196	CC	denotes	and
T9489	25197-25203	NN	denotes	extent
T9490	25204-25206	IN	denotes	of
T9491	25207-25220	NN	denotes	hyperactivity
T9492	25221-25223	IN	denotes	in
T9493	25224-25227	DT	denotes	the
T9494	25236-25240	NNS	denotes	mice
T9495	25228-25231	NN	denotes	tTA
T9496	25232-25235	NN	denotes	APP
T9497	25231-25232	HYPH	denotes	/
T9498	25240-25242	,	denotes	,
T9499	25242-25244	PRP	denotes	we
T9500	25256-25261	JJ	denotes	daily
T9501	25271-25277	NNS	denotes	levels
T9502	25262-25270	NN	denotes	activity
T9503	25278-25280	IN	denotes	in
T9504	25281-25287	JJ	denotes	double
T9505	25288-25298	JJ	denotes	transgenic
T9506	25299-25306	NNS	denotes	animals
T9507	25307-25312	IN	denotes	along
T9508	25313-25317	IN	denotes	with
T9509	25318-25323	PRP$	denotes	their
T9510	25360-25368	NNS	denotes	siblings
T9511	25324-25330	JJ	denotes	single
T9512	25331-25341	JJ	denotes	transgenic
T9513	25342-25345	CC	denotes	and
T9514	25346-25359	JJ	denotes	nontransgenic
T9515	25369-25374	VBG	denotes	using
T9516	25375-25379	CD	denotes	four
T9517	25380-25384	NN	denotes	beam
T9518	25379-25380	HYPH	denotes	-
T9519	25385-25391	NNS	denotes	frames
T9520	25392-25400	VBN	denotes	designed
T9521	25401-25403	TO	denotes	to
T9522	25404-25411	VB	denotes	monitor
T9523	25412-25422	NN	denotes	ambulation
T9524	25423-25429	IN	denotes	within
T9525	25430-25432	DT	denotes	an
T9526	25442-25446	NN	denotes	cage
T9527	25433-25441	VBN	denotes	enclosed
T9528	25446-25447	.	denotes	.
T9529	25447-25604	sentence	denotes	As shown in Figure 7, the double transgenic mice were up to 10-fold more active during the dark phase of the day–night cycle than any of the control groups.
T9530	25448-25450	IN	denotes	As
T9531	25451-25456	VBN	denotes	shown
T9532	25497-25501	VBD	denotes	were
T9533	25457-25459	IN	denotes	in
T9534	25460-25466	NN	denotes	Figure
T9535	25467-25468	CD	denotes	7
T9536	25468-25470	,	denotes	,
T9537	25470-25473	DT	denotes	the
T9538	25492-25496	NNS	denotes	mice
T9539	25474-25480	JJ	denotes	double
T9540	25481-25491	JJ	denotes	transgenic
T9541	25502-25504	IN	denotes	up
T9542	25508-25515	RB	denotes	10-fold
T9543	25505-25507	IN	denotes	to
T9544	25521-25527	JJ	denotes	active
T9545	25516-25520	RBR	denotes	more
T9546	25528-25534	IN	denotes	during
T9547	25535-25538	DT	denotes	the
T9548	25544-25549	NN	denotes	phase
T9549	25539-25543	NN	denotes	dark
T9550	25550-25552	IN	denotes	of
T9551	25553-25556	DT	denotes	the
T9552	25567-25572	NN	denotes	cycle
T9553	25557-25560	NN	denotes	day
T9554	25561-25566	NN	denotes	night
T9555	25560-25561	HYPH	denotes	–
T9556	25573-25577	IN	denotes	than
T9557	25578-25581	DT	denotes	any
T9558	25582-25584	IN	denotes	of
T9559	25585-25588	DT	denotes	the
T9560	25597-25603	NNS	denotes	groups
T9561	25589-25596	NN	denotes	control
T9562	25603-25604	.	denotes	.
T9563	25604-25726	sentence	denotes	Activity levels appeared to follow a relatively normal diurnal cycle, decreasing substantially during the daylight hours.
T9564	25605-25613	NN	denotes	Activity
T9565	25614-25620	NNS	denotes	levels
T9566	25621-25629	VBD	denotes	appeared
T9567	25630-25632	TO	denotes	to
T9568	25633-25639	VB	denotes	follow
T9569	25640-25641	DT	denotes	a
T9570	25668-25673	NN	denotes	cycle
T9571	25642-25652	RB	denotes	relatively
T9572	25653-25659	JJ	denotes	normal
T9573	25660-25667	JJ	denotes	diurnal
T9574	25673-25675	,	denotes	,
T9575	25675-25685	VBG	denotes	decreasing
T9576	25686-25699	RB	denotes	substantially
T9577	25700-25706	IN	denotes	during
T9578	25707-25710	DT	denotes	the
T9579	25720-25725	NNS	denotes	hours
T9580	25711-25719	NN	denotes	daylight
T9581	25725-25726	.	denotes	.
T9582	25726-25834	sentence	denotes	However, even during the light phase, the tTA/APP mice remained many-fold more active than normal controls.
T9583	25727-25734	RB	denotes	However
T9584	25782-25790	VBD	denotes	remained
T9585	25734-25736	,	denotes	,
T9586	25736-25740	RB	denotes	even
T9587	25741-25747	IN	denotes	during
T9588	25748-25751	DT	denotes	the
T9589	25758-25763	NN	denotes	phase
T9590	25752-25757	NN	denotes	light
T9591	25763-25765	,	denotes	,
T9592	25765-25768	DT	denotes	the
T9593	25777-25781	NNS	denotes	mice
T9594	25769-25772	NN	denotes	tTA
T9595	25773-25776	NN	denotes	APP
T9596	25772-25773	HYPH	denotes	/
T9597	25791-25800	RB	denotes	many-fold
T9598	25801-25805	RBR	denotes	more
T9599	25806-25812	JJ	denotes	active
T9600	25813-25817	IN	denotes	than
T9601	25818-25824	JJ	denotes	normal
T9602	25825-25833	NNS	denotes	controls
T9603	25833-25834	.	denotes	.
T9604	25834-25972	sentence	denotes	This behavior was partially, but not consistently, reversed by 1 mo of transgene suppression beginning at 4–5 mo of age (data not shown).
T9605	25835-25839	DT	denotes	This
T9606	25840-25848	NN	denotes	behavior
T9607	25886-25894	VBN	denotes	reversed
T9608	25849-25852	VBD	denotes	was
T9609	25853-25862	RB	denotes	partially
T9610	25862-25864	,	denotes	,
T9611	25864-25867	CC	denotes	but
T9612	25868-25871	RB	denotes	not
T9613	25872-25884	RB	denotes	consistently
T9614	25884-25886	,	denotes	,
T9615	25895-25897	IN	denotes	by
T9616	25898-25899	CD	denotes	1
T9617	25900-25902	NN	denotes	mo
T9618	25903-25905	IN	denotes	of
T9619	25906-25915	NN	denotes	transgene
T9620	25916-25927	NN	denotes	suppression
T9621	25928-25937	VBG	denotes	beginning
T9622	25938-25940	IN	denotes	at
T9623	25941-25942	CD	denotes	4
T9624	25943-25944	CD	denotes	5
T9625	25942-25943	SYM	denotes	–
T9626	25945-25947	NN	denotes	mo
T9627	25948-25950	IN	denotes	of
T9628	25951-25954	NN	denotes	age
T9629	25955-25956	-LRB-	denotes	(
T9630	25965-25970	VBN	denotes	shown
T9631	25956-25960	NNS	denotes	data
T9632	25961-25964	RB	denotes	not
T9633	25970-25971	-RRB-	denotes	)
T9634	25971-25972	.	denotes	.
T9635	25972-26056	sentence	denotes	In contrast, hyperactivity was completely abolished by rearing tTA/APP mice on dox.
T9636	25973-25975	IN	denotes	In
T9637	26015-26024	VBN	denotes	abolished
T9638	25976-25984	NN	denotes	contrast
T9639	25984-25986	,	denotes	,
T9640	25986-25999	NN	denotes	hyperactivity
T9641	26000-26003	VBD	denotes	was
T9642	26004-26014	RB	denotes	completely
T9643	26025-26027	IN	denotes	by
T9644	26028-26035	VBG	denotes	rearing
T9645	26036-26039	NN	denotes	tTA
T9646	26040-26043	NN	denotes	APP
T9647	26039-26040	HYPH	denotes	/
T9648	26044-26048	NNS	denotes	mice
T9649	26049-26051	IN	denotes	on
T9650	26052-26055	NN	denotes	dox
T9651	26055-26056	.	denotes	.
T9652	26056-26157	sentence	denotes	Animals born and raised on dox showed activity levels similar to the untreated controls (Figure 7C).
T9653	26057-26064	NNS	denotes	Animals
T9654	26088-26094	VBD	denotes	showed
T9655	26065-26069	VBN	denotes	born
T9656	26070-26073	CC	denotes	and
T9657	26074-26080	VBN	denotes	raised
T9658	26081-26083	IN	denotes	on
T9659	26084-26087	NN	denotes	dox
T9660	26095-26103	NN	denotes	activity
T9687	26223-26227	JJ	denotes	wild
T9688	26228-26232	NN	denotes	type
T9689	26227-26228	HYPH	denotes	-
T9690	26232-26234	,	denotes	,
T9691	26241-26248	VBN	denotes	altered
T9692	26259-26266	NNS	denotes	rhythms
T9693	26249-26258	JJ	denotes	circadian
T9694	26267-26271	IN	denotes	with
T9695	26272-26275	RB	denotes	far
T9696	26276-26280	JJR	denotes	less
T9697	26281-26292	NN	denotes	distinction
T9698	26293-26300	IN	denotes	between
T9699	26301-26306	PRP$	denotes	their
T9700	26335-26341	NNS	denotes	levels
T9701	26307-26310	NN	denotes	day
T9702	26310-26311	HYPH	denotes	-
T9703	26312-26315	CC	denotes	and
T9704	26316-26325	NN	denotes	nighttime
T9705	26326-26334	NN	denotes	activity
T9706	26341-26342	.	denotes	.
T9668	26153-26155	NN	denotes	7C
T9669	26146-26152	NN	denotes	Figure
T9670	26155-26156	-RRB-	denotes	)
T9671	26156-26157	.	denotes	.
T9672	26157-26342	sentence	denotes	Intriguingly, all of the dox-reared animals, both transgenic and wild-type, showed altered circadian rhythms with far less distinction between their day- and nighttime activity levels.
T9673	26158-26170	RB	denotes	Intriguingly
T9674	26234-26240	VBD	denotes	showed
T9675	26170-26172	,	denotes	,
T9676	26172-26175	DT	denotes	all
T9677	26176-26178	IN	denotes	of
T9678	26179-26182	DT	denotes	the
T9679	26194-26201	NNS	denotes	animals
T9680	26183-26186	NN	denotes	dox
T9681	26187-26193	VBN	denotes	reared
T9682	26186-26187	HYPH	denotes	-
T9683	26201-26203	,	denotes	,
T9684	26203-26207	CC	denotes	both
T9685	26208-26218	JJ	denotes	transgenic
T9686	26219-26222	CC	denotes	and
T9661	26104-26110	NNS	denotes	levels
T9662	26111-26118	JJ	denotes	similar
T9663	26119-26121	IN	denotes	to
T9664	26122-26125	DT	denotes	the
T9665	26136-26144	NNS	denotes	controls
T9666	26126-26135	JJ	denotes	untreated
T9667	26145-26146	-LRB-	denotes	(
R3967	T6511	T6512	nsubj	We,used
R3968	T6513	T6514	det	the,system
R3969	T6514	T6512	dobj	system,used
R3970	T6515	T6516	compound	tet,off
R3971	T6516	T6514	compound	off,system
R3972	T6517	T6516	punct	-,off
R3973	T6518	T6514	compound	transgene,system
R3974	T6519	T6520	aux	to,express
R3975	T6520	T6512	advcl	express,used
R3976	T6521	T6522	det	a,version
R3977	T6522	T6520	dobj	version,express
R3978	T6523	T6522	amod	double,version
R3979	T6524	T6522	compound	mutant,version
R3980	T6525	T6522	prep	of,version
R3981	T6526	T6527	amod	chimeric,huAPP695
R3982	T6527	T6525	pobj	huAPP695,of
R3983	T6528	T6527	compound	mo,huAPP695
R3984	T6529	T6527	punct	/,huAPP695
R3985	T6530	T6522	punct	(,version
R3986	T6531	T6532	compound	swe,KM570
R3987	T6532	T6522	appos	KM570,version
R3988	T6533	T6532	punct	/,KM570
R3989	T6534	T6532	compound	ind,KM570
R3990	T6535	T6532	punct	", ",KM570
R3991	T6536	T6532	conj	571NL,KM570
R3992	T6537	T6536	punct	", ",571NL
R3993	T6538	T6536	cc	and,571NL
R3994	T6539	T6536	conj	V617F,571NL
R3995	T6540	T6520	punct	),express
R3996	T6541	T6520	prep	from,express
R3997	T6542	T6543	det	a,promoter
R3998	T6543	T6541	pobj	promoter,from
R3999	T6544	T6545	npadvmod	tetracycline,responsive
R4000	T6545	T6543	amod	responsive,promoter
R4001	T6546	T6545	punct	-,responsive
R4002	T6547	T6548	punct	[,13
R4003	T6548	T6520	parataxis	13,express
R4004	T6549	T6548	nummod	12,13
R4005	T6550	T6548	punct	",",13
R4006	T6551	T6548	punct	],13
R4007	T6552	T6512	punct	.,used
R4008	T6554	T6555	amod	Transgenic,APP
R4009	T6555	T6556	compound	APP,expression
R4010	T6556	T6557	nsubjpass	expression,activated
R4011	T6558	T6557	auxpass	was,activated
R4012	T6559	T6557	prep	by,activated
R4013	T6560	T6559	pcomp	crossing,by
R4014	T6561	T6562	det	the,mice
R4015	T6562	T6560	dobj	mice,crossing
R4016	T6563	T6564	compound	APPswe,ind
R4017	T6564	T6562	compound	ind,mice
R4018	T6565	T6564	punct	/,ind
R4019	T6566	T6560	prep	to,crossing
R4020	T6567	T6566	pobj	animals,to
R4021	T6568	T6567	acl	producing,animals
R4022	T6569	T6568	dobj	tTA,producing
R4023	T6570	T6568	prep	under,producing
R4024	T6571	T6570	pobj	control,under
R4025	T6572	T6571	prep	of,control
R4026	T6573	T6574	det	the,promoter
R4027	T6574	T6572	pobj	promoter,of
R4028	T6575	T6574	compound	CaMKIIα,promoter
R4029	T6576	T6577	punct	[,16
R4030	T6577	T6568	parataxis	16,producing
R4031	T6578	T6577	punct	],16
R4032	T6579	T6557	punct	.,activated
R4033	T6581	T6582	prep	After,identified
R4034	T6583	T6584	amod	initial,screening
R4035	T6584	T6581	pobj	screening,After
R4036	T6585	T6584	prep	of,screening
R4037	T6586	T6585	pobj	founders,of
R4038	T6587	T6582	punct	", ",identified
R4039	T6588	T6582	nsubj	we,identified
R4040	T6589	T6590	nummod	four,lines
R4041	T6590	T6582	dobj	lines,identified
R4042	T6591	T6590	prep	of,lines
R4043	T6592	T6593	compound	tet,ind
R4044	T6593	T6597	compound	ind,mice
R4045	T6594	T6593	punct	-,ind
R4046	T6595	T6593	compound	APPswe,ind
R4047	T6596	T6593	punct	/,ind
R4048	T6597	T6591	pobj	mice,of
R4049	T6598	T6599	dep	that,produced
R4050	T6599	T6590	relcl	produced,lines
R4051	T6600	T6601	advmod	very,high
R4052	T6601	T6602	amod	high,levels
R4053	T6602	T6599	dobj	levels,produced
R4054	T6603	T6602	prep	of,levels
R4055	T6604	T6605	compound	transgene,product
R4056	T6605	T6603	pobj	product,of
R4057	T6606	T6599	prep	in,produced
R4058	T6607	T6606	pobj	offspring,in
R4059	T6608	T6607	acl	coexpressing,offspring
R4060	T6609	T6608	dobj	tTA,coexpressing
R4061	T6610	T6611	punct	(,Figures
R4062	T6611	T6599	parataxis	Figures,produced
R4063	T6612	T6611	nummod	1,Figures
R4064	T6613	T6611	cc	and,Figures
R4065	T6614	T6615	compound	Figure,S1
R4066	T6615	T6611	conj	S1,Figures
R4067	T6616	T6611	punct	),Figures
R4068	T6617	T6582	punct	.,identified
R4069	T6619	T6620	prep	Compared,estimated
R4070	T6621	T6619	prep	to,Compared
R4071	T6622	T6623	det	a,line
R4072	T6623	T6621	pobj	line,to
R4073	T6624	T6623	amod	standard,line
R4074	T6625	T6623	nmod	APP,line
R4075	T6626	T6623	amod	transgenic,line
R4076	T6627	T6623	acl	used,line
R4077	T6628	T6627	prep	for,used
R4078	T6629	T6630	amod	previous,studies
R4079	T6630	T6628	pobj	studies,for
R4080	T6631	T6630	compound	amyloid,studies
R4081	T6632	T6630	prep	by,studies
R4082	T6633	T6634	poss	our,laboratory
R4083	T6634	T6632	pobj	laboratory,by
R4084	T6635	T6636	punct	(,27
R4085	T6636	T6627	parataxis	27,used
R4086	T6637	T6638	compound	line,C3
R4087	T6638	T6636	dep	C3,27
R4088	T6639	T6638	punct	–,C3
R4089	T6640	T6638	nummod	3,C3
R4090	T6641	T6636	punct	;,27
R4091	T6642	T6636	punct	[,27
R4092	T6643	T6636	nummod	15,27
R4093	T6644	T6636	punct	",",27
R4094	T6645	T6636	nummod	26,27
R4095	T6646	T6636	punct	",",27
R4096	T6647	T6636	punct	],27
R4097	T6648	T6636	punct	),27
R4098	T6649	T6620	punct	", ",estimated
R4099	T6650	T6620	nsubj	we,estimated
R4100	T6651	T6652	mark	that,produce
R4101	T6652	T6620	ccomp	produce,estimated
R4102	T6653	T6654	det	the,lines
R4103	T6654	T6652	nsubj	lines,produce
R4104	T6655	T6654	nummod	four,lines
R4105	T6656	T6654	amod	controllable,lines
R4106	T6657	T6658	amod	transgenic,protein
R4107	T6658	T6652	dobj	protein,produce
R4108	T6659	T6658	compound	APP,protein
R4109	T6660	T6652	prep	at,produce
R4110	T6661	T6660	pcomp	10,at
R4111	T6662	T6661	punct	-,10
R4112	T6663	T6661	quantmod	to,10
R4113	T6664	T6661	advmod	30-fold,10
R4114	T6665	T6661	prep	over,10
R4115	T6666	T6667	amod	endogenous,levels
R4116	T6667	T6665	pobj	levels,over
R4117	T6668	T6669	punct	(,S1
R4118	T6669	T6652	parataxis	S1,produce
R4119	T6670	T6669	compound	Figure,S1
R4120	T6671	T6669	punct	),S1
R4121	T6672	T6620	punct	.,estimated
R4122	T6674	T6675	det	This,estimate
R4123	T6675	T6676	nsubjpass	estimate,confirmed
R4124	T6677	T6676	auxpass	was,confirmed
R4125	T6678	T6676	agent	by,confirmed
R4126	T6679	T6680	amod	direct,comparison
R4127	T6680	T6678	pobj	comparison,by
R4128	T6681	T6680	prep	of,comparison
R4129	T6682	T6683	compound	APP,levels
R4130	T6683	T6681	pobj	levels,of
R4131	T6684	T6680	prep	in,comparison
R4132	T6685	T6686	amod	nontransgenic,mice
R4133	T6686	T6684	pobj	mice,in
R4134	T6687	T6685	cc	and,nontransgenic
R4135	T6688	T6689	compound	tet,off
R4136	T6689	T6685	conj	off,nontransgenic
R4137	T6690	T6689	punct	-,off
R4138	T6691	T6686	compound	APP,mice
R4139	T6692	T6676	advcl	using,confirmed
R4140	T6693	T6694	det	an,antibody
R4141	T6694	T6692	dobj	antibody,using
R4142	T6695	T6696	dep	that,recognizes
R4143	T6696	T6694	relcl	recognizes,antibody
R4144	T6697	T6698	preconj	both,APP
R4145	T6698	T6696	dobj	APP,recognizes
R4146	T6699	T6698	amod	endogenous,APP
R4147	T6700	T6698	punct	(,APP
R4148	T6701	T6698	cc	and,APP
R4149	T6702	T6703	nmod	amyloid,protein
R4150	T6703	T6698	conj	protein,APP
R4151	T6704	T6705	npadvmod	precursor,like
R4152	T6705	T6703	amod	like,protein
R4153	T6706	T6705	punct	-,like
R4154	T6707	T6703	nummod	2,protein
R4155	T6708	T6703	punct	),protein
R4156	T6709	T6698	cc	and,APP
R4157	T6710	T6711	det	the,protein
R4158	T6711	T6698	conj	protein,APP
R4159	T6712	T6711	amod	transgenic,protein
R4160	T6713	T6714	punct	(,1D
R4161	T6714	T6711	parataxis	1D,protein
R4162	T6715	T6716	amod	monoclonal,22C11
R4163	T6716	T6714	dep	22C11,1D
R4164	T6717	T6716	compound	antibody,22C11
R4165	T6718	T6714	punct	;,1D
R4166	T6719	T6714	compound	Figure,1D
R4167	T6720	T6714	punct	),1D
R4168	T6721	T6676	punct	.,confirmed
R4169	T6723	T6724	advmod	Importantly,showed
R4170	T6725	T6724	punct	", ",showed
R4171	T6726	T6727	det	all,lines
R4172	T6727	T6724	nsubj	lines,showed
R4173	T6728	T6727	nummod	four,lines
R4174	T6729	T6727	amod	new,lines
R4175	T6730	T6727	prep	of,lines
R4176	T6731	T6732	compound	tet,off
R4177	T6732	T6734	compound	off,mice
R4178	T6733	T6732	punct	-,off
R4179	T6734	T6730	pobj	mice,of
R4180	T6735	T6734	compound	APP,mice
R4181	T6736	T6737	advmod	nearly,complete
R4182	T6737	T6738	amod	complete,suppression
R4183	T6738	T6724	dobj	suppression,showed
R4184	T6739	T6738	prep	of,suppression
R4185	T6740	T6741	det	the,transgene
R4186	T6741	T6739	pobj	transgene,of
R4187	T6742	T6724	prep	following,showed
R4188	T6743	T6744	compound	dox,treatment
R4189	T6744	T6742	pobj	treatment,following
R4190	T6745	T6746	punct	(,Figures
R4191	T6746	T6724	parataxis	Figures,showed
R4192	T6747	T6746	nummod	1,Figures
R4193	T6748	T6746	cc	and,Figures
R4194	T6749	T6750	compound	Figure,S1
R4195	T6750	T6746	conj	S1,Figures
R4196	T6751	T6746	punct	),Figures
R4197	T6752	T6724	punct	.,showed
R4198	T6754	T6755	nsubj	We,focused
R4199	T6756	T6755	prep	on,focused
R4200	T6757	T6756	pobj	one,on
R4201	T6758	T6757	prep	of,one
R4202	T6759	T6760	det	the,lines
R4203	T6760	T6758	pobj	lines,of
R4204	T6761	T6760	nummod	four,lines
R4205	T6762	T6757	punct	", ",one
R4206	T6763	T6757	appos	line,one
R4207	T6764	T6763	nummod	107,line
R4208	T6765	T6755	punct	", ",focused
R4209	T6766	T6767	aux	to,examine
R4210	T6767	T6755	advcl	examine,focused
R4211	T6768	T6767	prep	in,examine
R4212	T6769	T6770	amod	more,detail
R4213	T6770	T6768	pobj	detail,in
R4214	T6771	T6772	det	the,dependence
R4215	T6772	T6767	dobj	dependence,examine
R4216	T6773	T6772	compound	time,dependence
R4217	T6774	T6772	cc	and,dependence
R4218	T6775	T6772	conj	extent,dependence
R4219	T6776	T6775	prep	of,extent
R4220	T6777	T6778	compound	transgene,suppression
R4221	T6778	T6776	pobj	suppression,of
R4222	T6779	T6772	prep	following,dependence
R4223	T6780	T6781	preconj	either,treatment
R4224	T6781	T6779	pobj	treatment,following
R4225	T6782	T6781	amod	acute,treatment
R4226	T6783	T6782	cc	or,acute
R4227	T6784	T6782	conj	chronic,acute
R4228	T6785	T6781	prep	with,treatment
R4229	T6786	T6785	pobj	dox,with
R4230	T6787	T6755	punct	.,focused
R4231	T6789	T6790	nummod	Two,groups
R4232	T6790	T6794	nsubjpass	groups,compared
R4233	T6791	T6792	npadvmod	dox,treated
R4234	T6792	T6790	amod	treated,groups
R4235	T6793	T6792	punct	-,treated
R4236	T6794	T6796	ccomp	compared,harvested
R4237	T6795	T6794	auxpass	were,compared
R4238	T6797	T6794	prep	to,compared
R4239	T6798	T6799	nummod	two,groups
R4240	T6799	T6797	pobj	groups,to
R4241	T6800	T6799	amod	untreated,groups
R4242	T6801	T6796	punct	: ,harvested
R4243	T6802	T6803	nummod	one,group
R4244	T6803	T6804	nsubjpass	group,born
R4245	T6804	T6796	ccomp	born,harvested
R4246	T6805	T6803	prep	of,group
R4247	T6806	T6805	pobj	mice,of
R4248	T6807	T6804	auxpass	was,born
R4249	T6808	T6804	cc	and,born
R4250	T6809	T6804	conj	raised,born
R4251	T6810	T6809	prep	on,raised
R4252	T6811	T6810	pobj	dox,on
R4253	T6812	T6796	punct	", ",harvested
R4254	T6813	T6814	det	a,group
R4255	T6814	T6816	nsubjpass	group,treated
R4256	T6815	T6814	amod	second,group
R4257	T6816	T6796	ccomp	treated,harvested
R4258	T6817	T6816	auxpass	was,treated
R4259	T6818	T6816	prep	with,treated
R4260	T6819	T6818	pobj	dox,with
R4261	T6820	T6816	prep	for,treated
R4262	T6821	T6822	nummod	2,wk
R4263	T6822	T6820	pobj	wk,for
R4264	T6823	T6816	advcl	starting,treated
R4265	T6824	T6823	prep	at,starting
R4266	T6825	T6826	nummod	1,mo
R4267	T6826	T6824	pobj	mo,at
R4268	T6827	T6826	prep	of,mo
R4269	T6828	T6827	pobj	age,of
R4270	T6829	T6830	punct	(,wk
R4271	T6830	T6816	parataxis	wk,treated
R4272	T6831	T6830	nummod	4,wk
R4273	T6832	T6830	punct	+,wk
R4274	T6833	T6834	nummod	2,wk
R4275	T6834	T6835	compound	wk,dox
R4276	T6835	T6830	appos	dox,wk
R4277	T6836	T6830	punct	),wk
R4278	T6837	T6796	punct	;,harvested
R4279	T6838	T6839	nummod	two,groups
R4280	T6839	T6796	nsubjpass	groups,harvested
R4281	T6840	T6839	amod	untreated,groups
R4282	T6841	T6839	acl	kept,groups
R4283	T6842	T6841	prep	on,kept
R4284	T6843	T6844	amod	normal,chow
R4285	T6844	T6842	pobj	chow,on
R4286	T6845	T6796	auxpass	were,harvested
R4287	T6846	T6796	prep	at,harvested
R4288	T6847	T6848	preconj	either,4
R4289	T6848	T6849	nummod	4,wk
R4290	T6849	T6846	pobj	wk,at
R4291	T6850	T6848	cc	or,4
R4292	T6851	T6848	conj	6,4
R4293	T6852	T6849	prep	of,wk
R4294	T6853	T6852	pobj	age,of
R4295	T6854	T6796	punct	.,harvested
R4296	T6856	T6857	nsubj	Animals,harbored
R4297	T6858	T6856	acl	born,Animals
R4298	T6859	T6858	cc	and,born
R4299	T6860	T6858	conj	raised,born
R4300	T6861	T6860	prep	on,raised
R4301	T6862	T6861	pobj	dox,on
R4302	T6863	T6864	det	no,APP
R4303	T6864	T6857	dobj	APP,harbored
R4304	T6865	T6864	amod	transgenic,APP
R4305	T6866	T6867	punct	(,1A
R4306	T6867	T6857	parataxis	1A,harbored
R4307	T6868	T6867	compound	Figure,1A
R4308	T6869	T6867	punct	),1A
R4309	T6870	T6857	punct	.,harbored
R4310	T6872	T6873	prep	Following,reduced
R4311	T6874	T6875	advmod	as,2
R4312	T6875	T6878	nummod	2,wk
R4313	T6876	T6875	amod	little,2
R4314	T6877	T6875	quantmod	as,2
R4315	T6878	T6872	pobj	wk,Following
R4316	T6879	T6878	prep	of,wk
R4317	T6880	T6881	compound	dox,treatment
R4318	T6881	T6879	pobj	treatment,of
R4319	T6882	T6873	punct	", ",reduced
R4320	T6883	T6884	amod	transgenic,expression
R4321	T6884	T6873	nsubjpass	expression,reduced
R4322	T6885	T6884	compound	APP,expression
R4323	T6886	T6873	auxpass	was,reduced
R4324	T6887	T6873	prep	by,reduced
R4325	T6888	T6889	amod	more,95
R4326	T6889	T6891	nummod	95,%
R4327	T6890	T6889	quantmod	than,95
R4328	T6891	T6887	pobj	%,by
R4329	T6892	T6873	prep	compared,reduced
R4330	T6893	T6892	prep	to,compared
R4331	T6894	T6895	amod	pre-dox,levels
R4332	T6895	T6893	pobj	levels,to
R4333	T6896	T6873	punct	.,reduced
R4334	T6898	T6899	det	The,expression
R4335	T6899	T6901	nsubj	expression,represents
R4336	T6900	T6899	amod	residual,expression
R4337	T6902	T6899	acl	remaining,expression
R4338	T6903	T6902	prep	in,remaining
R4339	T6904	T6905	advmod	acutely,treated
R4340	T6905	T6906	amod	treated,mice
R4341	T6906	T6903	pobj	mice,in
R4342	T6907	T6908	amod	less,4
R4343	T6908	T6910	nummod	4,%
R4344	T6909	T6908	quantmod	than,4
R4345	T6910	T6901	dobj	%,represents
R4346	T6911	T6910	prep	of,%
R4347	T6912	T6913	det	the,protein
R4348	T6913	T6911	pobj	protein,of
R4349	T6914	T6913	amod	transgenic,protein
R4350	T6915	T6913	acl	produced,protein
R4351	T6916	T6915	prep	in,produced
R4352	T6917	T6918	det	the,absence
R4353	T6918	T6916	pobj	absence,in
R4354	T6919	T6918	prep	of,absence
R4355	T6920	T6919	pobj	dox,of
R4356	T6921	T6922	punct	(,1C
R4357	T6922	T6915	parataxis	1C,produced
R4358	T6923	T6922	compound	Figure,1C
R4359	T6924	T6922	punct	),1C
R4360	T6925	T6901	punct	", ",represents
R4361	T6926	T6901	cc	and,represents
R4362	T6927	T6928	advmod	likely,results
R4363	T6928	T6901	conj	results,represents
R4364	T6929	T6928	prep	from,results
R4365	T6930	T6931	amod	slight,leakage
R4366	T6931	T6929	pobj	leakage,from
R4367	T6932	T6931	prep	at,leakage
R4368	T6933	T6934	det	the,level
R4369	T6934	T6932	pobj	level,at
R4370	T6935	T6934	prep	of,level
R4371	T6936	T6935	pobj	transcription,of
R4372	T6937	T6938	punct	(,shown
R4373	T6938	T6928	parataxis	shown,results
R4374	T6939	T6938	nsubj	data,shown
R4375	T6940	T6938	neg	not,shown
R4376	T6941	T6938	punct	),shown
R4377	T6942	T6901	punct	.,represents
R4378	T6944	T6945	advmod	Importantly,was
R4379	T6946	T6945	punct	", ",was
R4380	T6947	T6948	det	the,amount
R4381	T6948	T6945	nsubj	amount,was
R4382	T6949	T6948	amod	total,amount
R4383	T6950	T6948	prep	of,amount
R4384	T6951	T6950	pobj	APP,of
R4385	T6952	T6953	punct	(,endogenous
R4386	T6953	T6951	parataxis	endogenous,APP
R4387	T6954	T6953	cc	plus,endogenous
R4388	T6955	T6953	conj	transgenic,endogenous
R4389	T6956	T6953	punct	),endogenous
R4390	T6957	T6951	cc	and,APP
R4391	T6958	T6959	amod	related,APLPs
R4392	T6959	T6951	conj	APLPs,APP
R4393	T6960	T6948	prep	in,amount
R4394	T6961	T6962	preconj	both,acute
R4395	T6962	T6963	amod	acute,animals
R4396	T6963	T6960	pobj	animals,in
R4397	T6964	T6962	cc	and,acute
R4398	T6965	T6966	advmod	chronically,treated
R4399	T6966	T6962	conj	treated,acute
R4400	T6967	T6968	advmod	statistically,indistinguishable
R4401	T6968	T6945	acomp	indistinguishable,was
R4402	T6969	T6968	prep	from,indistinguishable
R4403	T6970	T6969	pobj	that,from
R4404	T6971	T6970	prep	in,that
R4405	T6972	T6973	amod	nontransgenic,mice
R4406	T6973	T6971	pobj	mice,in
R4407	T6974	T6975	punct	(,1D
R4408	T6975	T6945	parataxis	1D,was
R4409	T6976	T6975	compound	Figure,1D
R4410	T6977	T6975	punct	;,1D
R4411	T6978	T6979	amod	statistical,analyses
R4412	T6979	T6980	nsubjpass	analyses,presented
R4413	T6980	T6975	ccomp	presented,1D
R4414	T6981	T6979	prep	for,analyses
R4415	T6982	T6981	pobj	experiments,for
R4416	T6983	T6982	prep	throughout,experiments
R4417	T6984	T6985	det	the,study
R4418	T6985	T6983	pobj	study,throughout
R4419	T6986	T6980	auxpass	are,presented
R4420	T6987	T6980	prep	in,presented
R4421	T6988	T6989	det	the,legends
R4422	T6989	T6987	pobj	legends,in
R4423	T6990	T6989	amod	accompanying,legends
R4424	T6991	T6989	compound	figure,legends
R4425	T6992	T6975	punct	),1D
R4426	T6993	T6945	punct	.,was
R4427	T6995	T6996	aux	To,ensure
R4428	T6996	T6997	advcl	ensure,measured
R4429	T6998	T6999	mark	that,suppressed
R4430	T6999	T6996	ccomp	suppressed,ensure
R4431	T7000	T7001	compound	Aβ,production
R4432	T7001	T6999	nsubjpass	production,suppressed
R4433	T7002	T6999	auxpass	was,suppressed
R4434	T7003	T6999	prep	in,suppressed
R4435	T7004	T7003	pobj	concert,in
R4436	T7005	T7004	prep	with,concert
R4437	T7006	T7007	det	the,inhibition
R4438	T7007	T7005	pobj	inhibition,with
R4439	T7008	T7009	npadvmod	dox,mediated
R4440	T7009	T7007	amod	mediated,inhibition
R4441	T7010	T7009	punct	-,mediated
R4442	T7011	T7007	prep	of,inhibition
R4443	T7012	T7013	poss	its,precursor
R4444	T7013	T7011	pobj	precursor,of
R4445	T7014	T7015	compound	APPswe,ind
R4446	T7015	T7013	appos	ind,precursor
R4447	T7016	T7015	punct	/,ind
R4448	T7017	T6997	punct	", ",measured
R4449	T7018	T6997	nsubj	we,measured
R4450	T7019	T7020	nmod	Aβ40,levels
R4451	T7020	T6997	dobj	levels,measured
R4452	T7021	T7019	cc	and,Aβ40
R4453	T7022	T7019	conj	Aβ42,Aβ40
R4454	T7023	T6997	prep	by,measured
R4455	T7024	T7023	pobj	ELISA,by
R4456	T7025	T6997	prep	in,measured
R4457	T7026	T7027	compound	forebrain,homogenates
R4458	T7027	T7025	pobj	homogenates,in
R4459	T7028	T7027	prep	from,homogenates
R4460	T7029	T7030	amod	young,animals
R4461	T7030	T7028	pobj	animals,from
R4462	T7031	T7032	compound	tet,off
R4463	T7032	T7030	compound	off,animals
R4464	T7033	T7032	punct	-,off
R4465	T7034	T6997	punct	.,measured
R4466	T7036	T7037	prep	At,lacked
R4467	T7038	T7039	nummod	1,mo
R4468	T7039	T7036	pobj	mo,At
R4469	T7040	T7039	prep	of,mo
R4470	T7041	T7040	pobj	age,of
R4471	T7042	T7037	punct	", ",lacked
R4472	T7043	T7044	det	the,mice
R4473	T7044	T7037	nsubj	mice,lacked
R4474	T7045	T7046	amod	visible,aggregates
R4475	T7046	T7037	dobj	aggregates,lacked
R4476	T7047	T7046	compound	amyloid,aggregates
R4477	T7048	T7049	dep	that,act
R4478	T7049	T7046	relcl	act,aggregates
R4479	T7050	T7049	aux	might,act
R4480	T7051	T7049	prep	as,act
R4481	T7052	T7053	det	an,reservoir
R4482	T7053	T7051	pobj	reservoir,as
R4483	T7054	T7053	amod	intractable,reservoir
R4484	T7055	T7053	prep	of,reservoir
R4485	T7056	T7055	pobj	peptide,of
R4486	T7057	T7053	acl	remaining,reservoir
R4487	T7058	T7057	prep	in,remaining
R4488	T7059	T7060	det	the,brain
R4489	T7060	T7058	pobj	brain,in
R4490	T7061	T7062	prep	after,suppressed
R4491	T7062	T7057	advcl	suppressed,remaining
R4492	T7063	T7064	det	the,transgene
R4493	T7064	T7062	nsubjpass	transgene,suppressed
R4494	T7065	T7062	aux	had,suppressed
R4495	T7066	T7062	auxpass	been,suppressed
R4496	T7067	T7037	punct	.,lacked
R4497	T7069	T7070	aux	To,ensure
R4498	T7070	T7072	advcl	ensure,performed
R4499	T7071	T7070	advmod	further,ensure
R4500	T7073	T7074	nsubj	we,detect
R4501	T7074	T7070	advcl	detect,ensure
R4502	T7075	T7074	aux	could,detect
R4503	T7076	T7077	det	any,aggregates
R4504	T7077	T7074	dobj	aggregates,detect
R4505	T7078	T7077	amod	such,aggregates
R4506	T7079	T7077	amod	insoluble,aggregates
R4507	T7080	T7081	dep	that,bias
R4508	T7081	T7077	relcl	bias,aggregates
R4509	T7082	T7081	aux	might,bias
R4510	T7083	T7084	poss	our,measure
R4511	T7084	T7081	dobj	measure,bias
R4512	T7085	T7084	prep	of,measure
R4513	T7086	T7085	pobj	changes,of
R4514	T7087	T7081	prep	in,bias
R4515	T7088	T7089	compound	peptide,synthesis
R4516	T7089	T7087	pobj	synthesis,in
R4517	T7090	T7072	punct	", ",performed
R4518	T7091	T7072	nsubj	we,performed
R4519	T7092	T7093	det	a,extraction
R4520	T7093	T7072	dobj	extraction,performed
R4521	T7094	T7093	amod	sequential,extraction
R4522	T7095	T7096	nummod	three,step
R4523	T7096	T7093	compound	step,extraction
R4524	T7097	T7096	punct	-,step
R4525	T7098	T7093	prep	with,extraction
R4526	T7099	T7098	pobj	PBS,with
R4527	T7100	T7099	punct	", ",PBS
R4528	T7101	T7102	nummod	2,%
R4529	T7102	T7103	compound	%,SDS
R4530	T7103	T7099	conj	SDS,PBS
R4531	T7104	T7103	punct	", ",SDS
R4532	T7105	T7103	cc	and,SDS
R4533	T7106	T7107	nummod	70,%
R4534	T7107	T7108	compound	%,FA
R4535	T7108	T7103	conj	FA,SDS
R4536	T7109	T7110	dep	that,separate
R4537	T7110	T7093	relcl	separate,extraction
R4538	T7111	T7110	aux	would,separate
R4539	T7112	T7110	dobj	peptides,separate
R4540	T7113	T7110	prep	by,separate
R4541	T7114	T7113	pobj	solubility,by
R4542	T7115	T7072	punct	.,performed
R4543	T7117	T7118	nsubj	We,compared
R4544	T7119	T7120	det	the,levels
R4545	T7120	T7118	dobj	levels,compared
R4546	T7121	T7120	prep	of,levels
R4547	T7122	T7123	amod	human,transgene
R4548	T7123	T7124	npadvmod	transgene,derived
R4549	T7124	T7126	amod	derived,Aβ40
R4550	T7125	T7124	punct	-,derived
R4551	T7126	T7121	pobj	Aβ40,of
R4552	T7127	T7126	cc	and,Aβ40
R4553	T7128	T7126	conj	Aβ42,Aβ40
R4554	T7129	T7120	prep	in,levels
R4555	T7130	T7131	amod	untreated,mice
R4556	T7131	T7129	pobj	mice,in
R4557	T7132	T7120	prep	at,levels
R4558	T7133	T7134	nummod	4,wk
R4559	T7134	T7132	pobj	wk,at
R4560	T7135	T7133	cc	and,4
R4561	T7136	T7133	conj	6,4
R4562	T7137	T7134	prep	of,wk
R4563	T7138	T7137	pobj	age,of
R4564	T7139	T7118	prep	to,compared
R4565	T7140	T7139	pobj	animals,to
R4566	T7141	T7142	dep	that,born
R4567	T7142	T7140	advcl	born,animals
R4568	T7143	T7142	aux	had,born
R4569	T7144	T7142	preconj	either,born
R4570	T7145	T7142	auxpass	been,born
R4571	T7146	T7142	cc	and,born
R4572	T7147	T7142	conj	raised,born
R4573	T7148	T7147	prep	on,raised
R4574	T7149	T7148	pobj	dox,on
R4575	T7150	T7142	cc	or,born
R4576	T7151	T7152	dep	that,left
R4577	T7152	T7142	conj	left,born
R4578	T7153	T7152	aux	had,left
R4579	T7154	T7152	auxpass	been,left
R4580	T7155	T7152	oprd	untreated,left
R4581	T7156	T7152	prep	for,left
R4582	T7157	T7158	nummod	4,wk
R4583	T7158	T7156	pobj	wk,for
R4584	T7159	T7152	cc	and,left
R4585	T7160	T7161	advmod	then,placed
R4586	T7161	T7152	conj	placed,left
R4587	T7162	T7161	prep	on,placed
R4588	T7163	T7164	compound	dox,chow
R4589	T7164	T7162	pobj	chow,on
R4590	T7165	T7161	prep	for,placed
R4591	T7166	T7167	nummod	2,wk
R4592	T7167	T7165	pobj	wk,for
R4593	T7168	T7169	amod	prior,to
R4594	T7169	T7167	prep	to,wk
R4595	T7170	T7169	pobj	harvest,to
R4596	T7171	T7172	punct	(,line
R4597	T7172	T7161	parataxis	line,placed
R4598	T7173	T7174	det	the,groups
R4599	T7174	T7172	dep	groups,line
R4600	T7175	T7174	amod	same,groups
R4601	T7176	T7174	acl	described,groups
R4602	T7177	T7176	advmod	above,described
R4603	T7178	T7176	prep	for,described
R4604	T7179	T7180	compound	immunoblot,analysis
R4605	T7180	T7178	pobj	analysis,for
R4606	T7181	T7180	prep	of,analysis
R4607	T7182	T7183	compound	APPswe,ind
R4608	T7183	T7185	compound	ind,levels
R4609	T7184	T7183	punct	/,ind
R4610	T7185	T7181	pobj	levels,of
R4611	T7186	T7172	punct	", ",line
R4612	T7187	T7172	nummod	107,line
R4613	T7188	T7172	punct	),line
R4614	T7189	T7118	punct	.,compared
R4615	T7191	T7192	advcl	Consistent,found
R4616	T7193	T7191	prep	with,Consistent
R4617	T7194	T7195	det	the,reduction
R4618	T7195	T7193	pobj	reduction,with
R4619	T7196	T7195	prep	in,reduction
R4620	T7197	T7198	amod	full,length
R4621	T7198	T7200	compound	length,synthesis
R4622	T7199	T7198	punct	-,length
R4623	T7200	T7196	pobj	synthesis,in
R4624	T7201	T7202	compound	APPswe,ind
R4625	T7202	T7200	compound	ind,synthesis
R4626	T7203	T7202	punct	/,ind
R4627	T7204	T7195	acl	shown,reduction
R4628	T7205	T7204	agent	by,shown
R4629	T7206	T7205	pobj	immunoblot,by
R4630	T7207	T7208	punct	(,see
R4631	T7208	T7191	parataxis	see,Consistent
R4632	T7209	T7208	dobj	Figure,see
R4633	T7210	T7209	nummod	1,Figure
R4634	T7211	T7208	punct	),see
R4635	T7212	T7192	punct	", ",found
R4636	T7213	T7192	nsubj	we,found
R4637	T7214	T7215	mark	that,suppressed
R4638	T7215	T7192	ccomp	suppressed,found
R4639	T7216	T7217	npadvmod	transgene,derived
R4640	T7217	T7219	amod	derived,levels
R4641	T7218	T7217	punct	-,derived
R4642	T7219	T7215	nsubjpass	levels,suppressed
R4643	T7220	T7219	compound	Aβ,levels
R4644	T7221	T7215	auxpass	were,suppressed
R4645	T7222	T7215	advmod	completely,suppressed
R4646	T7223	T7215	prep	in,suppressed
R4647	T7224	T7223	pobj	animals,in
R4648	T7225	T7224	acl	born,animals
R4649	T7226	T7225	cc	and,born
R4650	T7227	T7225	conj	raised,born
R4651	T7228	T7227	prep	on,raised
R4652	T7229	T7228	pobj	dox,on
R4653	T7230	T7215	punct	", ",suppressed
R4654	T7231	T7215	cc	and,suppressed
R4655	T7232	T7233	auxpass	were,reduced
R4656	T7233	T7215	conj	reduced,suppressed
R4657	T7234	T7233	advmod	sharply,reduced
R4658	T7235	T7233	prep	following,reduced
R4659	T7236	T7237	amod	acute,treatment
R4660	T7237	T7235	pobj	treatment,following
R4661	T7238	T7239	punct	(,wk
R4662	T7239	T7236	parataxis	wk,acute
R4663	T7240	T7239	nummod	2,wk
R4664	T7241	T7239	punct	),wk
R4665	T7242	T7237	amod	antibiotic,treatment
R4666	T7243	T7192	punct	.,found
R4667	T7245	T7246	prep	Compared,dropped
R4668	T7247	T7245	prep	to,Compared
R4669	T7248	T7249	det	the,levels
R4670	T7249	T7247	pobj	levels,to
R4671	T7250	T7249	prep	in,levels
R4672	T7251	T7252	amod	untreated,mice
R4673	T7252	T7250	pobj	mice,in
R4674	T7253	T7254	nummod	4,wk
R4675	T7254	T7256	npadvmod	wk,old
R4676	T7255	T7254	punct	-,wk
R4677	T7256	T7252	amod	old,mice
R4678	T7257	T7256	punct	-,old
R4679	T7258	T7246	punct	", ",dropped
R4680	T7259	T7260	npadvmod	PBS,soluble
R4681	T7260	T7262	amod	soluble,Aβ42
R4682	T7261	T7260	punct	-,soluble
R4683	T7262	T7246	nsubj	Aβ42,dropped
R4684	T7263	T7246	prep	by,dropped
R4685	T7264	T7265	nummod	95.2,%
R4686	T7265	T7263	pobj	%,by
R4687	T7266	T7246	prep	following,dropped
R4688	T7267	T7268	nummod	2,wk
R4689	T7268	T7266	pobj	wk,following
R4690	T7269	T7268	prep	of,wk
R4691	T7270	T7271	compound	dox,treatment
R4692	T7271	T7269	pobj	treatment,of
R4693	T7272	T7246	cc	and,dropped
R4694	T7273	T7274	prep	by,with
R4695	T7274	T7246	conj	with,dropped
R4696	T7275	T7276	nummod	99.2,%
R4697	T7276	T7273	pobj	%,by
R4698	T7277	T7278	amod	chronic,treatment
R4699	T7278	T7274	pobj	treatment,with
R4700	T7279	T7280	punct	(,2A
R4701	T7280	T7274	parataxis	2A,with
R4702	T7281	T7280	compound	Figure,2A
R4703	T7282	T7280	punct	),2A
R4704	T7283	T7246	punct	.,dropped
R4705	T7285	T7286	advmod	Similarly,decreased
R4706	T7287	T7286	punct	", ",decreased
R4707	T7288	T7289	npadvmod	SDS,soluble
R4708	T7289	T7291	amod	soluble,Aβ42
R4709	T7290	T7289	punct	-,soluble
R4710	T7291	T7286	nsubj	Aβ42,decreased
R4711	T7292	T7286	prep	by,decreased
R4712	T7293	T7294	nummod	75.2,%
R4713	T7294	T7292	pobj	%,by
R4714	T7295	T7294	cc	and,%
R4715	T7296	T7297	nummod	94.8,%
R4716	T7297	T7294	conj	%,%
R4717	T7298	T7286	prep	following,decreased
R4718	T7299	T7300	nummod	2,wk
R4719	T7300	T7302	nmod	wk,treatment
R4720	T7301	T7300	punct	-,wk
R4721	T7302	T7298	pobj	treatment,following
R4722	T7303	T7300	cc	or,wk
R4723	T7304	T7300	conj	lifelong,wk
R4724	T7305	T7302	compound	dox,treatment
R4725	T7306	T7307	punct	(,2B
R4726	T7307	T7286	parataxis	2B,decreased
R4727	T7308	T7307	compound	Figure,2B
R4728	T7309	T7307	punct	),2B
R4729	T7310	T7286	punct	.,decreased
R4730	T7312	T7313	advmod	Only,fraction
R4731	T7313	T7316	nsubj	fraction,revealed
R4732	T7314	T7313	det	the,fraction
R4733	T7315	T7313	compound	FA,fraction
R4734	T7317	T7318	det	a,pool
R4735	T7318	T7316	dobj	pool,revealed
R4736	T7319	T7318	amod	small,pool
R4737	T7320	T7321	npadvmod	dox,resistant
R4738	T7321	T7318	amod	resistant,pool
R4739	T7322	T7321	punct	-,resistant
R4740	T7323	T7318	prep	of,pool
R4741	T7324	T7323	pobj	peptide,of
R4742	T7325	T7318	prep	in,pool
R4743	T7326	T7327	advmod	acutely,treated
R4744	T7327	T7328	amod	treated,animals
R4745	T7328	T7325	pobj	animals,in
R4746	T7329	T7330	dep	that,believe
R4747	T7330	T7318	relcl	believe,pool
R4748	T7331	T7330	nsubj	we,believe
R4749	T7332	T7330	advcl	represents,believe
R4750	T7333	T7334	amod	stable,aggregates
R4751	T7334	T7332	dobj	aggregates,represents
R4752	T7335	T7334	amod	predeposit,aggregates
R4753	T7336	T7337	dep	that,accumulated
R4754	T7337	T7334	relcl	accumulated,aggregates
R4755	T7338	T7337	aux	have,accumulated
R4756	T7339	T7337	advmod	already,accumulated
R4757	T7340	T7337	prep	by,accumulated
R4758	T7341	T7342	nummod	4,wk
R4759	T7342	T7340	pobj	wk,by
R4760	T7343	T7342	prep	of,wk
R4761	T7344	T7343	pobj	age,of
R4762	T7345	T7346	advmod	when,begun
R4763	T7346	T7337	advcl	begun,accumulated
R4764	T7347	T7346	nsubjpass	treatment,begun
R4765	T7348	T7346	auxpass	was,begun
R4766	T7349	T7350	punct	(,2C
R4767	T7350	T7316	parataxis	2C,revealed
R4768	T7351	T7350	compound	Figure,2C
R4769	T7352	T7350	punct	),2C
R4770	T7353	T7316	punct	.,revealed
R4771	T7355	T7356	advmod	Indeed,harbor
R4772	T7357	T7356	punct	", ",harbor
R4773	T7358	T7356	nsubj	animals,harbor
R4774	T7359	T7360	dep	that,born
R4775	T7360	T7358	relcl	born,animals
R4776	T7361	T7360	auxpass	were,born
R4777	T7362	T7360	cc	and,born
R4778	T7363	T7360	conj	raised,born
R4779	T7364	T7363	prep	on,raised
R4780	T7365	T7364	pobj	dox,on
R4781	T7366	T7356	aux	did,harbor
R4782	T7367	T7356	neg	not,harbor
R4783	T7368	T7369	det	this,reservoir
R4784	T7369	T7356	dobj	reservoir,harbor
R4785	T7370	T7369	prep	of,reservoir
R4786	T7371	T7372	npadvmod	treatment,resistant
R4787	T7372	T7374	amod	resistant,peptide
R4788	T7373	T7372	punct	-,resistant
R4789	T7374	T7370	pobj	peptide,of
R4790	T7375	T7356	punct	", ",harbor
R4791	T7376	T7356	prep	with,harbor
R4792	T7377	T7378	nummod	96.3,%
R4793	T7378	T7379	npadvmod	%,less
R4794	T7379	T7380	amod	less,Aβ42
R4795	T7380	T7376	pobj	Aβ42,with
R4796	T7381	T7380	prep	than,Aβ42
R4797	T7382	T7383	amod	untreated,mice
R4798	T7383	T7381	pobj	mice,than
R4799	T7384	T7385	nummod	4,wk
R4800	T7385	T7387	npadvmod	wk,old
R4801	T7386	T7385	punct	-,wk
R4802	T7387	T7383	amod	old,mice
R4803	T7388	T7387	punct	-,old
R4804	T7389	T7356	punct	.,harbor
R4805	T7391	T7392	nsubj	Measurement,demonstrated
R4806	T7393	T7391	prep	of,Measurement
R4807	T7394	T7395	amod	total,Aβ
R4808	T7395	T7393	pobj	Aβ,of
R4809	T7396	T7391	prep	in,Measurement
R4810	T7397	T7398	advmod	chronically,treated
R4811	T7398	T7399	amod	treated,mice
R4812	T7399	T7396	pobj	mice,in
R4813	T7400	T7391	punct	", ",Measurement
R4814	T7401	T7391	prep	including,Measurement
R4815	T7402	T7403	amod	endogenous,peptide
R4816	T7403	T7401	pobj	peptide,including
R4817	T7404	T7402	cc	and,endogenous
R4818	T7405	T7406	npadvmod	transgene,derived
R4819	T7406	T7402	conj	derived,endogenous
R4820	T7407	T7406	punct	-,derived
R4821	T7408	T7392	punct	", ",demonstrated
R4822	T7409	T7410	mark	that,reduced
R4823	T7410	T7392	ccomp	reduced,demonstrated
R4824	T7411	T7412	compound	Aβ,levels
R4825	T7412	T7410	nsubjpass	levels,reduced
R4826	T7413	T7412	prep	in,levels
R4827	T7414	T7415	compound	tet,off
R4828	T7415	T7417	compound	off,mice
R4829	T7416	T7415	punct	-,off
R4830	T7417	T7413	pobj	mice,in
R4831	T7418	T7417	compound	APP,mice
R4832	T7419	T7410	auxpass	were,reduced
R4833	T7420	T7410	prep	to,reduced
R4834	T7421	T7422	det	the,level
R4835	T7422	T7420	pobj	level,to
R4836	T7423	T7422	prep	of,level
R4837	T7424	T7425	amod	endogenous,peptide
R4838	T7425	T7423	pobj	peptide,of
R4839	T7426	T7422	acl	found,level
R4840	T7427	T7426	prep	in,found
R4841	T7428	T7429	amod	nontransgenic,animals
R4842	T7429	T7427	pobj	animals,in
R4843	T7430	T7431	punct	(,2D
R4844	T7431	T7410	parataxis	2D,reduced
R4845	T7432	T7431	compound	Figure,2D
R4846	T7433	T7431	punct	),2D
R4847	T7434	T7392	punct	.,demonstrated
R4848	T7436	T7437	advcl	Taken,indicate
R4849	T7438	T7436	advmod	together,Taken
R4850	T7439	T7436	prep	with,Taken
R4851	T7440	T7441	det	the,data
R4852	T7441	T7439	pobj	data,with
R4853	T7442	T7441	compound	immunoblotting,data
R4854	T7443	T7441	prep	for,data
R4855	T7444	T7445	amod	full,length
R4856	T7445	T7447	compound	length,ind
R4857	T7446	T7445	punct	-,length
R4858	T7447	T7443	pobj	ind,for
R4859	T7448	T7447	compound	APPswe,ind
R4860	T7449	T7447	punct	/,ind
R4861	T7450	T7437	punct	", ",indicate
R4862	T7451	T7452	det	the,measurements
R4863	T7452	T7437	nsubj	measurements,indicate
R4864	T7453	T7452	compound	ELISA,measurements
R4865	T7454	T7455	mark	that,leads
R4866	T7455	T7437	ccomp	leads,indicate
R4867	T7543	T7544	advmod	as,young
R4868	T7456	T7457	npadvmod	dox,mediated
R4869	T7544	T7542	amod	young,mice
R4870	T7545	T7544	prep	as,young
R4871	T7457	T7459	amod	mediated,suppression
R4872	T7546	T7547	nummod	8,wk
R4873	T7547	T7545	pobj	wk,as
R4874	T7548	T7547	prep	of,wk
R4875	T7458	T7457	punct	-,mediated
R4876	T7549	T7548	pobj	age,of
R4877	T7550	T7551	punct	(,shown
R4878	T7459	T7455	nsubj	suppression,leads
R4879	T7551	T7539	parataxis	shown,seen
R4880	T7552	T7551	nsubj	data,shown
R4881	T7460	T7459	prep	of,suppression
R4882	T7553	T7551	neg	not,shown
R4883	T7554	T7551	punct	),shown
R4884	T7555	T7539	punct	.,seen
R4885	T7461	T7462	amod	transgenic,synthesis
R4886	T7557	T7558	nsubjpass	Plaques,limited
R4887	T7462	T7460	pobj	synthesis,of
R4888	T7559	T7558	auxpass	were,limited
R4889	T7463	T7464	compound	APPswe,ind
R4890	T7560	T7558	prep	to,limited
R4891	T7561	T7562	det	the,forebrain
R4892	T7562	T7560	pobj	forebrain,to
R4893	T7464	T7462	compound	ind,synthesis
R4894	T7563	T7562	punct	", ",forebrain
R4895	T7564	T7562	prep	including,forebrain
R4896	T7565	T7566	det	the,cortex
R4897	T7465	T7464	punct	/,ind
R4898	T7566	T7564	pobj	cortex,including
R4899	T7567	T7566	punct	", ",cortex
R4900	T7568	T7566	conj	hippocampus,cortex
R4901	T7569	T7568	punct	", ",hippocampus
R4902	T7570	T7571	amod	olfactory,bulb
R4903	T7466	T7455	prep	to,leads
R4904	T7571	T7568	conj	bulb,hippocampus
R4905	T7572	T7571	punct	", ",bulb
R4906	T7573	T7571	cc	and,bulb
R4907	T7467	T7468	amod	parallel,reduction
R4908	T7574	T7571	conj	striatum,bulb
R4909	T7468	T7466	pobj	reduction,to
R4910	T7575	T7562	punct	", ",forebrain
R4911	T7576	T7577	advmod	where,known
R4912	T7577	T7562	relcl	known,forebrain
R4913	T7578	T7579	det	the,promoter
R4914	T7469	T7468	prep	of,reduction
R4915	T7579	T7577	nsubjpass	promoter,known
R4916	T7580	T7579	compound	CaMKIIα,promoter
R4917	T7581	T7577	auxpass	is,known
R4918	T7470	T7471	compound	Aβ,levels
R4919	T7582	T7583	aux	to,be
R4920	T7583	T7577	xcomp	be,known
R4921	T7584	T7585	advmod	most,active
R4922	T7471	T7469	pobj	levels,of
R4923	T7585	T7583	acomp	active,be
R4924	T7586	T7587	punct	[,28
R4925	T7472	T7437	punct	.,indicate
R4926	T7474	T7475	det	The,data
R4927	T7587	T7577	parataxis	28,known
R4928	T7588	T7587	nummod	16,28
R4929	T7589	T7587	punct	",",28
R4930	T7475	T7477	nsubj	data,confirmed
R4931	T7590	T7587	punct	],28
R4932	T7591	T7592	punct	(,S2
R4933	T7476	T7475	compound	ELISA,data
R4934	T7592	T7558	parataxis	S2,limited
R4935	T7593	T7592	compound	Figure,S2
R4936	T7594	T7592	punct	),S2
R4937	T7478	T7477	advmod	also,confirmed
R4938	T7595	T7558	punct	.,limited
R4939	T7597	T7598	prep	By,became
R4940	T7479	T7480	mark	that,led
R4941	T7599	T7600	nummod	6,mo
R4942	T7480	T7477	ccomp	led,confirmed
R4943	T7600	T7597	pobj	mo,By
R4944	T7601	T7600	prep	of,mo
R4945	T7602	T7601	pobj	age,of
R4946	T7481	T7480	nsubj	incorporation,led
R4947	T7603	T7598	punct	", ",became
R4948	T7604	T7605	compound	amyloid,burden
R4949	T7605	T7598	nsubj	burden,became
R4950	T7606	T7598	acomp	severe,became
R4951	T7607	T7598	punct	", ",became
R4952	T7608	T7598	advcl	covering,became
R4953	T7482	T7481	prep	of,incorporation
R4954	T7609	T7610	amod	large,areas
R4955	T7610	T7608	dobj	areas,covering
R4956	T7611	T7610	prep	of,areas
R4957	T7483	T7484	det	the,mutations
R4958	T7612	T7613	det	the,cortex
R4959	T7613	T7611	pobj	cortex,of
R4960	T7614	T7613	cc	and,cortex
R4961	T7484	T7482	pobj	mutations,of
R4962	T7615	T7613	conj	hippocampus,cortex
R4963	T7485	T7484	amod	Swedish,mutations
R4964	T7616	T7617	punct	(,S3
R4965	T7617	T7608	parataxis	S3,covering
R4966	T7618	T7617	compound	Figure,S3
R4967	T7619	T7617	punct	),S3
R4968	T7486	T7485	cc	and,Swedish
R4969	T7620	T7598	punct	.,became
R4970	T7487	T7485	conj	Indiana,Swedish
R4971	T7622	T7623	det	No,lesions
R4972	T7623	T7624	nsubjpass	lesions,seen
R4973	T7488	T7480	prep	to,led
R4974	T7625	T7624	auxpass	were,seen
R4975	T7626	T7624	prep	in,seen
R4976	T7489	T7490	amod	high,levels
R4977	T7627	T7628	det	the,cerebellum
R4978	T7628	T7626	pobj	cerebellum,in
R4979	T7629	T7628	cc	or,cerebellum
R4980	T7490	T7488	pobj	levels,to
R4981	T7630	T7631	compound	brain,stem
R4982	T7631	T7628	conj	stem,cerebellum
R4983	T7632	T7633	advmod	even,at
R4984	T7491	T7490	prep	of,levels
R4985	T7633	T7624	prep	at,seen
R4986	T7634	T7635	amod	late,ages
R4987	T7635	T7633	pobj	ages,at
R4988	T7492	T7491	pobj	Aβ42,of
R4989	T7636	T7624	punct	", ",seen
R4990	T7637	T7624	advcl	consistent,seen
R4991	T7493	T7490	punct	", ",levels
R4992	T7638	T7637	prep	with,consistent
R4993	T7639	T7640	npadvmod	CaMKIIα,controlled
R4994	T7640	T7642	amod	controlled,expression
R4995	T7494	T7495	dep	which,predicted
R4996	T7641	T7640	punct	-,controlled
R4997	T7642	T7638	pobj	expression,with
R4998	T7643	T7642	compound	transgene,expression
R4999	T7495	T7490	relcl	predicted,levels
R5000	T7644	T7624	punct	.,seen
R5001	T7496	T7495	nsubj	we,predicted
R5002	T7646	T7647	prep	Unlike,are
R5003	T7648	T7649	dep	what,thought
R5004	T7497	T7498	aux	would,induce
R5005	T7498	T7495	advcl	induce,predicted
R5006	T7499	T7500	amod	rapid,formation
R5007	T7500	T7498	dobj	formation,induce
R5008	T7649	T7646	pcomp	thought,Unlike
R5009	T7650	T7649	auxpass	is,thought
R5010	T7501	T7500	compound	plaque,formation
R5011	T7502	T7498	prep	in,induce
R5012	T7651	T7652	aux	to,occur
R5013	T7503	T7504	amod	untreated,animals
R5014	T7652	T7649	xcomp	occur,thought
R5015	T7504	T7502	pobj	animals,in
R5016	T7653	T7652	prep	in,occur
R5017	T7654	T7655	det	the,disease
R5018	T7505	T7477	punct	.,confirmed
R5019	T7655	T7653	pobj	disease,in
R5020	T7656	T7655	amod	human,disease
R5021	T7657	T7647	punct	", ",are
R5022	T7507	T7508	parataxis	Histological,characterization
R5023	T7658	T7659	det	the,plaques
R5024	T7659	T7647	nsubj	plaques,are
R5025	T7660	T7659	amod	first,plaques
R5026	T7508	T7509	nsubj	characterization,revealed
R5027	T7661	T7659	amod	visible,plaques
R5028	T7662	T7659	prep	in,plaques
R5029	T7663	T7664	det	the,mice
R5030	T7510	T7508	prep	of,characterization
R5031	T7664	T7662	pobj	mice,in
R5032	T7665	T7666	compound	tet,off
R5033	T7666	T7664	compound	off,mice
R5034	T7511	T7512	amod	double,mice
R5035	T7667	T7666	punct	-,off
R5036	T7668	T7664	compound	APP,mice
R5037	T7669	T7670	amod	fibrillar,cored
R5038	T7512	T7510	pobj	mice,of
R5039	T7670	T7672	amod	cored,deposits
R5040	T7671	T7670	punct	-,cored
R5041	T7672	T7647	attr	deposits,are
R5042	T7513	T7512	amod	transgenic,mice
R5043	T7673	T7647	punct	.,are
R5044	T7514	T7515	punct	(,APPswe
R5045	T7675	T7676	nsubj	We,noted
R5046	T7515	T7512	parataxis	APPswe,mice
R5047	T7677	T7676	aux	have,noted
R5048	T7516	T7515	nmod	CaMKIIα,APPswe
R5049	T7517	T7515	punct	-,APPswe
R5050	T7678	T7679	det	the,appearance
R5051	T7679	T7676	dobj	appearance,noted
R5052	T7518	T7515	nmod	tTA,APPswe
R5053	T7680	T7679	amod	same,appearance
R5054	T7681	T7679	amod	early,appearance
R5055	T7682	T7679	prep	of,appearance
R5056	T7683	T7684	amod	cored,deposits
R5057	T7684	T7682	pobj	deposits,of
R5058	T7519	T7515	punct	×,APPswe
R5059	T7685	T7679	prep	in,appearance
R5060	T7686	T7687	amod	other,lines
R5061	T7520	T7515	compound	tet,APPswe
R5062	T7687	T7685	pobj	lines,in
R5063	T7688	T7687	prep	of,lines
R5064	T7521	T7515	punct	-,APPswe
R5065	T7689	T7690	nmod	APP,mice
R5066	T7690	T7688	pobj	mice,of
R5067	T7691	T7690	amod	transgenic,mice
R5068	T7522	T7515	punct	/,APPswe
R5069	T7692	T7693	dep	that,harbor
R5070	T7693	T7687	relcl	harbor,lines
R5071	T7523	T7515	amod	ind,APPswe
R5072	T7694	T7695	det	the,mutation
R5073	T7695	T7693	dobj	mutation,harbor
R5074	T7696	T7695	amod	Swedish,mutation
R5075	T7524	T7515	punct	),APPswe
R5076	T7697	T7698	punct	[,27
R5077	T7698	T7676	parataxis	27,noted
R5078	T7525	T7526	amod	early,onset
R5079	T7699	T7698	punct	],27
R5080	T7700	T7676	punct	.,noted
R5081	T7526	T7528	compound	onset,formation
R5082	T7702	T7703	compound	Diffuse,plaques
R5083	T7703	T7704	nsubj	plaques,were
R5084	T7527	T7526	punct	-,onset
R5085	T7705	T7704	acomp	apparent,were
R5086	T7706	T7704	prep	in,were
R5087	T7528	T7509	dobj	formation,revealed
R5088	T7707	T7708	nummod	6,mo
R5089	T7708	T7710	npadvmod	mo,old
R5090	T7529	T7528	compound	amyloid,formation
R5091	T7709	T7708	punct	-,mo
R5092	T7710	T7712	amod	old,mice
R5093	T7711	T7710	punct	-,old
R5094	T7530	T7509	prep	in,revealed
R5095	T7712	T7706	pobj	mice,in
R5096	T7713	T7714	compound	tTA,APP
R5097	T7714	T7712	compound	APP,mice
R5098	T7531	T7532	det	all,lines
R5099	T7715	T7714	punct	/,APP
R5100	T7716	T7704	punct	", ",were
R5101	T7717	T7704	cc	and,were
R5102	T7532	T7530	pobj	lines,in
R5103	T7718	T7704	conj	became,were
R5104	T7719	T7720	advmod	relatively,abundant
R5105	T7533	T7532	nummod	four,lines
R5106	T7720	T7718	acomp	abundant,became
R5107	T7721	T7718	prep	by,became
R5108	T7722	T7723	nummod	9,mo
R5109	T7723	T7721	pobj	mo,by
R5110	T7724	T7723	prep	of,mo
R5111	T7725	T7724	pobj	age,of
R5112	T7534	T7532	amod	new,lines
R5113	T7726	T7704	punct	.,were
R5114	T7728	T7729	prep	At,were
R5115	T7535	T7509	punct	.,revealed
R5116	T7730	T7731	amod	older,ages
R5117	T7537	T7538	compound	Amyloid,plaques
R5118	T7731	T7728	pobj	ages,At
R5119	T7732	T7731	punct	(,ages
R5120	T7733	T7734	quantmod	9,12
R5121	T7538	T7539	nsubjpass	plaques,seen
R5122	T7734	T7736	nummod	12,mo
R5123	T7735	T7734	punct	–,12
R5124	T7736	T7731	appos	mo,ages
R5125	T7540	T7539	auxpass	were,seen
R5126	T7737	T7729	punct	),were
R5127	T7738	T7739	compound	amyloid,deposits
R5128	T7739	T7729	nsubj	deposits,were
R5129	T7541	T7539	prep	in,seen
R5130	T7740	T7729	acomp	visible,were
R5131	T7741	T7729	prep	in,were
R5132	T7742	T7743	det	the,thalamus
R5133	T7542	T7541	pobj	mice,in
R5134	T7743	T7741	pobj	thalamus,in
R5136	T7744	T7743	punct	", ",thalamus
R5137	T7745	T7746	dep	which,observed
R5138	T7746	T7743	relcl	observed,thalamus
R5139	T7747	T7746	aux	has,observed
R5140	T7748	T7746	advmod	also,observed
R5141	T7756	T7757	det	the,promoter
R5142	T7749	T7746	auxpass	been,observed
R5143	T7750	T7746	prep	in,observed
R5144	T7751	T7750	pobj	mice,in
R5145	T7757	T7755	pobj	promoter,via
R5146	T7752	T7751	acl	expressing,mice
R5147	T7753	T7754	compound	mutant,APP
R5148	T7754	T7752	dobj	APP,expressing
R5149	T7758	T7757	nmod	Thy,promoter
R5150	T7759	T7758	punct	-,Thy
R5151	T7760	T7758	nummod	1,Thy
R5152	T7761	T7729	punct	.,were
R5153	T7763	T7764	det	The,presence
R5154	T7861	T7859	amod	expressing,line
R5155	T7862	T7859	punct	(,line
R5156	T7863	T7859	appos	line,line
R5157	T7764	T7765	nsubjpass	presence,attributed
R5158	T7864	T7863	nummod	885,line
R5159	T7865	T7855	punct	),Animals
R5160	T7866	T7855	acl	maintained,Animals
R5161	T7867	T7866	prep	on,maintained
R5162	T7868	T7867	pobj	dox,on
R5163	T7869	T7866	prep	for,maintained
R5164	T7870	T7869	prep	up,for
R5165	T7871	T7870	prep	to,up
R5166	T7766	T7764	prep	of,presence
R5167	T7872	T7873	nummod	1,y
R5168	T7873	T7871	pobj	y,to
R5169	T7874	T7875	det	no,pathology
R5170	T7875	T7856	dobj	pathology,harbored
R5171	T7876	T7875	compound	amyloid,pathology
R5172	T7767	T7768	compound	amyloid,pathology
R5173	T7877	T7878	punct	(,shown
R5174	T7878	T7875	parataxis	shown,pathology
R5175	T7768	T7766	pobj	pathology,of
R5176	T7879	T7878	nsubj	data,shown
R5177	T7880	T7878	neg	not,shown
R5178	T7881	T7875	punct	),pathology
R5179	T7769	T7764	prep	in,presence
R5180	T7882	T7856	punct	", ",harbored
R5181	T7883	T7856	advcl	indicating,harbored
R5182	T7770	T7771	det	this,region
R5183	T7884	T7885	mark	that,provide
R5184	T7885	T7883	ccomp	provide,indicating
R5185	T7886	T7887	amod	residual,leakage
R5186	T7771	T7769	pobj	region,in
R5187	T7887	T7885	nsubj	leakage,provide
R5188	T7888	T7887	prep	of,leakage
R5189	T7772	T7765	aux	has,attributed
R5190	T7889	T7890	compound	transgene,expression
R5191	T7890	T7888	pobj	expression,of
R5192	T7891	T7887	prep	in,leakage
R5193	T7773	T7765	auxpass	been,attributed
R5194	T7892	T7893	det	the,presence
R5195	T7893	T7891	pobj	presence,in
R5196	T7894	T7893	prep	of,presence
R5197	T7774	T7765	prep	to,attributed
R5198	T7895	T7894	pobj	dox,of
R5199	T7896	T7885	aux	does,provide
R5200	T7897	T7885	neg	not,provide
R5201	T7775	T7776	amod	axonal,transport
R5202	T7898	T7899	amod	sufficient,peptide
R5203	T7899	T7885	dobj	peptide,provide
R5204	T7900	T7899	compound	Aβ,peptide
R5205	T7776	T7774	pobj	transport,to
R5206	T7901	T7902	aux	to,induce
R5207	T7902	T7899	acl	induce,peptide
R5208	T7903	T7904	compound	amyloid,formation
R5209	T7777	T7776	prep	of,transport
R5210	T7904	T7902	dobj	formation,induce
R5211	T7905	T7906	advmod	even,over
R5212	T7906	T7902	prep	over,induce
R5213	T7907	T7908	amod	long,periods
R5214	T7908	T7906	pobj	periods,over
R5215	T7778	T7779	compound	APP,Aβ
R5216	T7909	T7856	punct	.,harbored
R5217	T7911	T7912	aux	To,mimic
R5218	T7779	T7777	pobj	Aβ,of
R5219	T7912	T7913	advcl	mimic,raised
R5220	T7780	T7779	punct	/,Aβ
R5221	T7914	T7915	amod	therapeutic,intervention
R5222	T7915	T7912	dobj	intervention,mimic
R5223	T7916	T7912	prep	with,mimic
R5224	T7781	T7776	prep	to,transport
R5225	T7917	T7916	pobj	inhibitors,with
R5226	T7918	T7917	prep	of,inhibitors
R5227	T7782	T7783	det	the,terminals
R5228	T7919	T7920	compound	Aβ,production
R5229	T7920	T7918	pobj	production,of
R5230	T7921	T7913	punct	", ",raised
R5231	T7783	T7781	pobj	terminals,to
R5232	T7922	T7913	nsubj	we,raised
R5233	T7923	T7924	det	a,group
R5234	T7784	T7783	prep	of,terminals
R5235	T7924	T7913	dobj	group,raised
R5236	T7925	T7924	prep	of,group
R5237	T7785	T7786	amod	cortical,neurons
R5238	T7926	T7927	nummod	25,mice
R5239	T7927	T7925	pobj	mice,of
R5240	T7928	T7929	amod	double,transgenic
R5241	T7786	T7784	pobj	neurons,of
R5242	T7929	T7927	amod	transgenic,mice
R5243	T7930	T7931	punct	(,APP
R5244	T7787	T7783	prep	in,terminals
R5245	T7931	T7927	parataxis	APP,mice
R5246	T7932	T7931	nmod	CaMKIIα,APP
R5247	T7933	T7931	punct	-,APP
R5248	T7788	T7789	det	the,thalamus
R5249	T7934	T7931	nmod	tTA,APP
R5250	T7935	T7931	punct	×,APP
R5251	T7789	T7787	pobj	thalamus,in
R5252	T7936	T7931	appos	line,APP
R5253	T7790	T7791	punct	[,29
R5254	T7791	T7765	parataxis	29,attributed
R5255	T7937	T7936	nummod	107,line
R5256	T7938	T7931	punct	),APP
R5257	T7792	T7791	punct	],29
R5258	T7939	T7913	prep	on,raised
R5259	T7940	T7941	amod	normal,food
R5260	T7941	T7939	pobj	food,on
R5261	T7793	T7765	punct	.,attributed
R5262	T7942	T7913	prep	until,raised
R5263	T7943	T7944	nummod	6,mo
R5264	T7944	T7942	pobj	mo,until
R5265	T7945	T7944	prep	of,mo
R5266	T7795	T7796	advmod	Most,importantly
R5267	T7946	T7945	pobj	age,of
R5268	T7947	T7913	punct	", ",raised
R5269	T7948	T7949	advmod	when,knew
R5270	T7796	T7797	advmod	importantly,developed
R5271	T7949	T7913	advcl	knew,raised
R5272	T7950	T7949	nsubj	we,knew
R5273	T7798	T7797	punct	", ",developed
R5274	T7951	T7952	compound	amyloid,formation
R5275	T7952	T7953	nsubj	formation,was
R5276	T7953	T7949	advcl	was,knew
R5277	T7799	T7800	advmod	only,mice
R5278	T7954	T7953	advmod	already,was
R5279	T7955	T7956	advmod	well,underway
R5280	T7956	T7953	acomp	underway,was
R5281	T7800	T7797	nsubj	mice,developed
R5282	T7957	T7953	prep	in,was
R5283	T7958	T7959	det	the,brain
R5284	T7801	T7802	amod	double,transgenic
R5285	T7959	T7957	pobj	brain,in
R5286	T7960	T7913	punct	.,raised
R5287	T7802	T7800	amod	transgenic,mice
R5288	T7962	T7963	prep	At,switched
R5289	T7803	T7800	punct	", ",mice
R5290	T7964	T7965	nummod	6,mo
R5291	T7965	T7962	pobj	mo,At
R5292	T7804	T7800	acl	expressing,mice
R5293	T7966	T7963	punct	", ",switched
R5294	T7805	T7806	preconj	both,transgenes
R5295	T7806	T7804	dobj	transgenes,expressing
R5296	T7967	T7963	nsubjpass	half,switched
R5297	T7968	T7967	prep	of,half
R5298	T7807	T7806	det	the,transgenes
R5299	T7969	T7970	det	the,animals
R5300	T7970	T7968	pobj	animals,of
R5301	T7808	T7806	nmod	tTA,transgenes
R5302	T7971	T7963	auxpass	were,switched
R5303	T7809	T7808	cc	and,tTA
R5304	T7972	T7963	prep	from,switched
R5305	T7973	T7974	amod	normal,chow
R5306	T7974	T7972	pobj	chow,from
R5307	T7810	T7808	conj	APP,tTA
R5308	T7975	T7963	prep	to,switched
R5309	T7976	T7975	pobj	food,to
R5310	T7811	T7797	punct	", ",developed
R5311	T7977	T7976	acl	containing,food
R5312	T7978	T7977	dobj	dox,containing
R5313	T7979	T7977	prep	at,containing
R5314	T7980	T7981	nummod	200,mg
R5315	T7981	T7979	pobj	mg,at
R5316	T7812	T7813	compound	amyloid,lesions
R5317	T7982	T7983	punct	/,kg
R5318	T7983	T7981	prep	kg,mg
R5319	T7984	T7985	mark	until,sacrificed
R5320	T7813	T7797	dobj	lesions,developed
R5321	T7985	T7963	advcl	sacrificed,switched
R5322	T7986	T7985	nsubjpass	they,sacrificed
R5323	T7987	T7985	auxpass	were,sacrificed
R5324	T7814	T7797	punct	.,developed
R5325	T7988	T7985	prep	at,sacrificed
R5326	T7989	T7990	nummod	9,mo
R5327	T7816	T7817	amod	Single,transgenic
R5328	T7990	T7988	pobj	mo,at
R5329	T7991	T7989	cc	or,9
R5330	T7992	T7989	conj	12,9
R5331	T7993	T7990	prep	of,mo
R5332	T7817	T7818	amod	transgenic,mice
R5333	T7994	T7993	pobj	age,of
R5334	T7995	T7963	punct	.,switched
R5335	T7818	T7819	nsubj	mice,showed
R5336	T7997	T7998	det	The,animals
R5337	T7998	T8001	nsubjpass	animals,kept
R5338	T7820	T7821	quantmod	up,15
R5339	T7999	T7998	amod	remaining,animals
R5340	T8000	T7998	compound	control,animals
R5341	T7821	T7823	nummod	15,mo
R5342	T8002	T8001	auxpass	were,kept
R5343	T8003	T8001	prep	on,kept
R5344	T8004	T8005	amod	standard,chow
R5345	T7822	T7821	quantmod	to,15
R5346	T8005	T8003	pobj	chow,on
R5347	T8006	T8007	punct	(,untreated
R5348	T7823	T7818	npadvmod	mo,mice
R5349	T8007	T8001	parataxis	untreated,kept
R5350	T8008	T8007	punct	),untreated
R5351	T8009	T8001	punct	.,kept
R5352	T7824	T7823	prep	of,mo
R5353	T8011	T8012	prep	In,created
R5354	T7825	T7824	pobj	age,of
R5355	T8013	T8011	pobj	all,In
R5356	T8014	T8012	punct	", ",created
R5357	T7826	T7827	det	no,sign
R5358	T8015	T8016	nummod	four,cohorts
R5359	T7827	T7819	dobj	sign,showed
R5360	T8016	T8012	nsubjpass	cohorts,created
R5361	T7828	T7827	prep	of,sign
R5362	T8017	T8012	auxpass	were,created
R5363	T8018	T8012	punct	: ,created
R5364	T7829	T7828	pobj	pathology,of
R5365	T8019	T8020	nummod	6,mo
R5366	T8020	T8012	dobj	mo,created
R5367	T8021	T8020	amod	untreated,mo
R5368	T8022	T8023	punct	(,7
R5369	T7830	T7831	punct	(,S3
R5370	T8023	T8020	parataxis	7,mo
R5371	T7831	T7819	parataxis	S3,showed
R5372	T8024	T8023	nsubj	n,7
R5373	T8025	T8023	punct	=,7
R5374	T7832	T7831	compound	Figure,S3
R5375	T8026	T8023	punct	),7
R5376	T8027	T8020	punct	", ",mo
R5377	T7833	T7831	punct	),S3
R5378	T8028	T8029	nummod	9,mo
R5379	T8029	T8020	conj	mo,mo
R5380	T8030	T8029	amod	untreated,mo
R5381	T7834	T7819	punct	.,showed
R5382	T8031	T8032	punct	(,5
R5383	T8032	T8029	parataxis	5,mo
R5384	T7836	T7837	advmod	Similarly,prevented
R5385	T8033	T8032	nsubj	n,5
R5386	T8034	T8032	punct	=,5
R5387	T8035	T8032	punct	),5
R5388	T7838	T7837	punct	", ",prevented
R5389	T8036	T8029	punct	", ",mo
R5390	T8037	T8038	nummod	6,mo
R5391	T8038	T8029	conj	mo,mo
R5392	T8039	T8038	punct	+,mo
R5393	T7839	T7840	compound	amyloid,pathology
R5394	T8040	T8041	nummod	3,mo
R5395	T7840	T7837	nsubjpass	pathology,prevented
R5396	T7841	T7837	aux	can,prevented
R5397	T8041	T8038	appos	mo,mo
R5398	T7842	T7837	auxpass	be,prevented
R5399	T8042	T8038	amod	treated,mo
R5400	T8043	T8044	punct	(,8
R5401	T7843	T7837	advmod	completely,prevented
R5402	T8044	T8038	parataxis	8,mo
R5403	T8045	T8044	nsubj	n,8
R5404	T7844	T7837	prep	in,prevented
R5405	T8046	T8044	punct	=,8
R5406	T8047	T8044	punct	),8
R5407	T8048	T8038	punct	", ",mo
R5408	T7845	T7846	amod	double,transgenic
R5409	T8049	T8038	cc	and,mo
R5410	T8050	T8051	nummod	6,mo
R5411	T8051	T8038	conj	mo,mo
R5412	T7846	T7847	amod	transgenic,animals
R5413	T8052	T8051	punct	+,mo
R5414	T8053	T8054	nummod	6,mo
R5415	T7847	T7844	pobj	animals,in
R5419	T8057	T8051	parataxis	5,mo
R5420	T8058	T8057	nsubj	n,5
R5421	T7848	T7847	acl	born,animals
R5422	T8059	T8057	punct	=,5
R5423	T8060	T8057	punct	),5
R5424	T8061	T8012	punct	.,created
R5425	T7849	T7848	cc	and,born
R5426	T8063	T8064	amod	Full,suppression
R5427	T7850	T7848	conj	raised,born
R5428	T8064	T8065	nsubjpass	suppression,confirmed
R5429	T8066	T8067	punct	(,%
R5430	T7851	T7850	prep	on,raised
R5431	T8067	T8064	parataxis	%,suppression
R5432	T8068	T8069	punct	>,95
R5433	T8069	T8067	nummod	95,%
R5434	T7852	T7851	pobj	dox,on
R5435	T8070	T8067	punct	),%
R5436	T8071	T8064	prep	of,suppression
R5437	T7853	T7837	punct	.,prevented
R5438	T7855	T7856	nsubj	Animals,harbored
R5439	T7857	T7855	prep	from,Animals
R5440	T8072	T8073	amod	transgenic,levels
R5441	T8073	T8071	pobj	levels,of
R5442	T8074	T8075	compound	APPswe,ind
R5443	T7858	T7859	poss	our,line
R5444	T8075	T8073	compound	ind,levels
R5445	T8076	T8075	punct	/,ind
R5446	T8077	T8073	prep	in,levels
R5447	T7859	T7857	pobj	line,from
R5448	T8078	T8079	det	the,animals
R5449	T8079	T8077	pobj	animals,in
R5450	T7860	T7861	amod	highest,expressing
R5451	T8080	T8081	npadvmod	dox,treated
R5452	T8081	T8079	amod	treated,animals
R5453	T8178	T8176	pobj	ind,of
R5454	T8082	T8081	punct	-,treated
R5455	T8083	T8065	auxpass	was,confirmed
R5456	T8084	T8065	prep	by,confirmed
R5457	T8085	T8084	pobj	immunoblot,by
R5458	T8086	T8087	punct	(,Figure
R5459	T8179	T8178	punct	/,ind
R5460	T8087	T8065	parataxis	Figure,confirmed
R5461	T8088	T8087	nummod	3,Figure
R5462	T8089	T8087	punct	),Figure
R5463	T8180	T8171	cc	and,life
R5464	T8090	T8065	punct	.,confirmed
R5465	T8181	T8182	poss	its,fragments
R5466	T8092	T8093	aux	To,ensure
R5467	T8093	T8094	advcl	ensure,treated
R5468	T8182	T8171	conj	fragments,life
R5469	T8095	T8096	mark	that,suppressed
R5470	T8096	T8093	ccomp	suppressed,ensure
R5471	T8097	T8098	det	the,transgene
R5472	T8098	T8096	nsubjpass	transgene,suppressed
R5473	T8099	T8096	aux	could,suppressed
R5474	T8183	T8182	amod	processed,fragments
R5475	T8100	T8096	auxpass	be,suppressed
R5476	T8101	T8102	advmod	as,rapidly
R5477	T8102	T8096	advmod	rapidly,suppressed
R5478	T8184	T8185	npadvmod	C,terminal
R5479	T8103	T8096	prep	in,suppressed
R5480	T8104	T8105	nummod	6,mo
R5481	T8105	T8107	npadvmod	mo,old
R5482	T8185	T8182	amod	terminal,fragments
R5483	T8106	T8105	punct	-,mo
R5484	T8107	T8109	amod	old,mice
R5485	T8108	T8107	punct	-,old
R5486	T8186	T8185	punct	-,terminal
R5487	T8187	T8188	advmod	relatively,short
R5488	T8188	T8169	acomp	short,are
R5489	T8109	T8103	pobj	mice,in
R5490	T8110	T8109	prep	with,mice
R5491	T8111	T8112	amod	fulminant,pathology
R5492	T8189	T8190	punct	(,3D
R5493	T8112	T8110	pobj	pathology,with
R5494	T8113	T8114	mark	as,can
R5495	T8190	T8144	parataxis	3D,suppressed
R5496	T8114	T8096	advcl	can,suppressed
R5497	T8115	T8114	nsubj	it,can
R5498	T8191	T8190	compound	Figure,3D
R5499	T8116	T8114	prep	in,can
R5500	T8117	T8118	amod	young,animals
R5501	T8192	T8190	punct	),3D
R5502	T8118	T8116	pobj	animals,in
R5503	T8119	T8118	punct	", ",animals
R5504	T8120	T8118	amod	predeposit,animals
R5505	T8121	T8094	punct	", ",treated
R5506	T8122	T8094	nsubj	we,treated
R5507	T8123	T8124	det	an,set
R5508	T8193	T8144	punct	.,suppressed
R5509	T8124	T8094	dobj	set,treated
R5510	T8125	T8124	amod	additional,set
R5511	T8126	T8124	prep	of,set
R5512	T8195	T8196	compound	Tissue,sections
R5513	T8127	T8128	nummod	6,mo
R5514	T8128	T8130	npadvmod	mo,old
R5515	T8129	T8128	punct	-,mo
R5516	T8130	T8132	amod	old,animals
R5517	T8196	T8197	nsubjpass	sections,stained
R5518	T8131	T8130	punct	-,old
R5519	T8132	T8126	pobj	animals,of
R5520	T8198	T8196	prep	from,sections
R5521	T8133	T8132	prep	with,animals
R5522	T8134	T8133	pobj	dox,with
R5523	T8135	T8124	prep	for,set
R5524	T8136	T8137	nummod	1,wk
R5525	T8137	T8135	pobj	wk,for
R5526	T8138	T8139	amod	prior,to
R5527	T8139	T8124	prep	to,set
R5528	T8199	T8200	det	each,animal
R5529	T8140	T8139	pobj	harvest,to
R5530	T8141	T8094	punct	.,treated
R5531	T8200	T8198	pobj	animal,from
R5532	T8143	T8144	advmod	Importantly,suppressed
R5533	T8201	T8200	prep	in,animal
R5534	T8145	T8144	punct	", ",suppressed
R5535	T8146	T8147	preconj	both,ind
R5536	T8147	T8150	npadvmod	ind,terminal
R5537	T8202	T8203	det	the,groups
R5538	T8148	T8147	compound	APPswe,ind
R5539	T8149	T8147	punct	/,ind
R5540	T8150	T8156	amod	terminal,levels
R5541	T8203	T8201	pobj	groups,in
R5542	T8151	T8147	cc	and,ind
R5543	T8152	T8153	compound	APP,C
R5544	T8153	T8147	conj	C,ind
R5545	T8154	T8153	punct	–,C
R5546	T8204	T8203	nummod	four,groups
R5547	T8155	T8150	punct	-,terminal
R5548	T8156	T8144	nsubjpass	levels,suppressed
R5549	T8157	T8156	compound	fragment,levels
R5550	T8205	T8203	compound	treatment,groups
R5551	T8158	T8144	auxpass	were,suppressed
R5552	T8159	T8144	advmod	fully,suppressed
R5553	T8206	T8197	auxpass	were,stained
R5554	T8160	T8144	prep	after,suppressed
R5555	T8161	T8162	advmod	only,wk
R5556	T8162	T8160	pobj	wk,after
R5557	T8207	T8197	prep	for,stained
R5558	T8163	T8162	nummod	1,wk
R5559	T8164	T8162	prep	of,wk
R5560	T8208	T8209	compound	amyloid,pathology
R5561	T8165	T8164	pobj	treatment,of
R5562	T8166	T8144	punct	", ",suppressed
R5563	T8167	T8144	advcl	indicating,suppressed
R5564	T8209	T8207	pobj	pathology,for
R5565	T8168	T8169	mark	that,are
R5566	T8169	T8167	ccomp	are,indicating
R5567	T8170	T8171	det	the,life
R5568	T8210	T8197	agent	by,stained
R5569	T8171	T8169	nsubj	life,are
R5570	T8172	T8173	advmod	in,vivo
R5571	T8211	T8212	compound	Hirano,silver
R5572	T8173	T8171	amod	vivo,life
R5573	T8174	T8171	compound	half,life
R5574	T8175	T8171	punct	-,life
R5575	T8176	T8171	prep	of,life
R5576	T8177	T8178	compound	APPswe,ind
R5577	T8212	T8210	pobj	silver,by
R5578	T8213	T8212	punct	", ",silver
R5579	T8214	T8215	compound	Campbell,Switzer
R5580	T8215	T8217	compound	Switzer,silver
R5581	T8216	T8215	punct	-,Switzer
R5582	T8284	T8283	prep	of,that
R5583	T8285	T8286	det	the,cohort
R5584	T8217	T8212	conj	silver,silver
R5585	T8286	T8284	pobj	cohort,of
R5586	T8287	T8288	nummod	6,mo
R5587	T8218	T8217	punct	", ",silver
R5588	T8288	T8289	npadvmod	mo,untreated
R5589	T8289	T8286	amod	untreated,cohort
R5590	T8290	T8256	punct	", ",resembled
R5591	T8219	T8220	compound	thioflavin,S
R5592	T8291	T8256	prep	despite,resembled
R5593	T8292	T8293	det	the,difference
R5594	T8220	T8217	conj	S,silver
R5595	T8293	T8291	pobj	difference,despite
R5596	T8294	T8293	amod	significant,difference
R5597	T8295	T8293	compound	age,difference
R5598	T8221	T8220	punct	-,S
R5599	T8296	T8293	prep	between,difference
R5600	T8297	T8298	det	the,groups
R5601	T8222	T8220	punct	", ",S
R5602	T8298	T8296	pobj	groups,between
R5603	T8299	T8298	amod	treated,groups
R5604	T8300	T8299	cc	and,treated
R5605	T8223	T8220	cc	and,S
R5606	T8301	T8299	conj	untreated,treated
R5607	T8302	T8303	punct	(,Figures
R5608	T8303	T8256	parataxis	Figures,resembled
R5609	T8224	T8225	compound	Aβ,immunohistochemistry
R5610	T8304	T8303	nummod	4,Figures
R5611	T8305	T8303	cc	and,Figures
R5612	T8306	T8307	compound	Figure,S3
R5613	T8225	T8220	conj	immunohistochemistry,S
R5614	T8307	T8303	conj	S3,Figures
R5615	T8308	T8303	punct	),Figures
R5616	T8226	T8197	punct	.,stained
R5617	T8309	T8256	punct	.,resembled
R5618	T8311	T8312	advmod	Well,formed
R5619	T8228	T8229	mark	As,expected
R5620	T8312	T8314	amod	formed,plaques
R5621	T8313	T8312	punct	-,formed
R5622	T8314	T8315	nsubj	plaques,remained
R5623	T8229	T8230	advcl	expected,displayed
R5624	T8316	T8315	prep	in,remained
R5625	T8317	T8318	det	the,animals
R5626	T8318	T8316	pobj	animals,in
R5627	T8319	T8318	amod	treated,animals
R5628	T8320	T8315	prep	after,remained
R5629	T8231	T8230	punct	", ",displayed
R5630	T8321	T8322	nummod	6,mo
R5631	T8322	T8320	pobj	mo,after
R5632	T8323	T8322	prep	of,mo
R5633	T8324	T8325	compound	transgene,suppression
R5634	T8232	T8233	det	the,cohort
R5635	T8325	T8323	pobj	suppression,of
R5636	T8326	T8315	punct	", ",remained
R5637	T8233	T8230	nsubj	cohort,displayed
R5638	T8327	T8328	advmod	even,given
R5639	T8328	T8315	advcl	given,remained
R5640	T8329	T8328	mark	though,given
R5641	T8234	T8235	nummod	6,mo
R5642	T8330	T8331	advmod	as,much
R5643	T8331	T8332	amod	much,time
R5644	T8332	T8328	nsubjpass	time,given
R5645	T8235	T8233	nmod	mo,cohort
R5646	T8333	T8328	auxpass	was,given
R5647	T8334	T8335	aux	to,clear
R5648	T8335	T8328	advcl	clear,given
R5649	T8236	T8233	amod	untreated,cohort
R5650	T8336	T8337	det	the,lesions
R5651	T8337	T8335	dobj	lesions,clear
R5652	T8338	T8339	mark	as,taken
R5653	T8237	T8238	amod	moderate,pathology
R5654	T8339	T8328	advcl	taken,given
R5655	T8340	T8339	nsubj	they,taken
R5656	T8238	T8230	dobj	pathology,displayed
R5657	T8341	T8339	aux	had,taken
R5658	T8342	T8343	aux	to,form
R5659	T8343	T8339	advcl	form,taken
R5660	T8239	T8238	compound	amyloid,pathology
R5661	T8344	T8315	punct	.,remained
R5662	T8346	T8347	advmod	Moreover,remained
R5663	T8240	T8230	punct	", ",displayed
R5664	T8348	T8347	punct	", ",remained
R5665	T8241	T8230	cc	and,displayed
R5666	T8349	T8350	det	both,types
R5667	T8350	T8347	nsubj	types,remained
R5668	T8351	T8350	prep	of,types
R5669	T8242	T8243	det	the,cohort
R5670	T8352	T8351	pobj	amyloid,of
R5671	T8353	T8350	punct	", ",types
R5672	T8354	T8350	amod	diffuse,types
R5673	T8243	T8247	nsubj	cohort,progressed
R5674	T8355	T8354	cc	and,diffuse
R5675	T8356	T8354	conj	fibrillar,diffuse
R5676	T8244	T8245	nummod	9,mo
R5677	T8357	T8347	punct	", ",remained
R5678	T8358	T8347	acomp	intact,remained
R5679	T8359	T8347	prep	throughout,remained
R5680	T8360	T8359	pobj	treatment,throughout
R5681	T8361	T8347	punct	.,remained
R5682	T8245	T8243	nmod	mo,cohort
R5683	T8363	T8364	advcl	Using,found
R5684	T8365	T8366	det	the,stain
R5685	T8246	T8243	amod	untreated,cohort
R5686	T8247	T8230	conj	progressed,displayed
R5687	T8248	T8247	prep	to,progressed
R5688	T8366	T8363	dobj	stain,Using
R5689	T8367	T8368	compound	Campbell,Switzer
R5690	T8249	T8250	det	a,burden
R5691	T8368	T8366	compound	Switzer,stain
R5692	T8369	T8368	punct	-,Switzer
R5693	T8370	T8366	compound	silver,stain
R5694	T8250	T8248	pobj	burden,to
R5695	T8371	T8372	aux	to,distinguish
R5696	T8372	T8363	advcl	distinguish,Using
R5697	T8251	T8250	amod	severe,burden
R5698	T8373	T8374	amod	different,forms
R5699	T8374	T8372	dobj	forms,distinguish
R5700	T8252	T8250	compound	amyloid,burden
R5701	T8375	T8374	prep	of,forms
R5702	T8376	T8375	pobj	amyloid,of
R5703	T8377	T8364	punct	", ",found
R5704	T8253	T8247	punct	.,progressed
R5705	T8378	T8364	nsubj	we,found
R5706	T8379	T8380	amod	diffuse,plaques
R5707	T8255	T8256	prep	In,resembled
R5708	T8380	T8381	nsubj	plaques,were
R5709	T8381	T8364	advcl	were,found
R5710	T8382	T8383	advmod	as,persistent
R5711	T8383	T8381	acomp	persistent,were
R5712	T8257	T8255	pobj	contrast,In
R5713	T8384	T8383	prep	as,persistent
R5714	T8385	T8386	amod	cored,deposits
R5715	T8386	T8384	pobj	deposits,as
R5716	T8258	T8256	punct	", ",resembled
R5717	T8387	T8388	punct	(,S4
R5718	T8388	T8364	parataxis	S4,found
R5719	T8389	T8388	compound	Figure,S4
R5720	T8259	T8260	det	the,extent
R5721	T8260	T8256	nsubj	extent,resembled
R5722	T8261	T8260	prep	of,extent
R5723	T8390	T8388	punct	),S4
R5724	T8262	T8263	compound	amyloid,pathology
R5725	T8391	T8364	punct	.,found
R5726	T8263	T8261	pobj	pathology,of
R5727	T8393	T8394	nsubj	It,was
R5728	T8395	T8394	advmod	nevertheless,was
R5729	T8396	T8394	acomp	clear,was
R5730	T8397	T8398	mark	that,halted
R5731	T8398	T8394	ccomp	halted,was
R5732	T8264	T8260	prep	in,extent
R5733	T8399	T8400	npadvmod	dox,induced
R5734	T8400	T8402	amod	induced,suppression
R5735	T8401	T8400	punct	-,induced
R5736	T8265	T8264	pobj	mice,in
R5737	T8402	T8398	nsubj	suppression,halted
R5738	T8403	T8402	prep	of,suppression
R5739	T8266	T8265	prep	from,mice
R5740	T8404	T8405	amod	transgenic,APP
R5741	T8405	T8403	pobj	APP,of
R5742	T8406	T8398	aux	had,halted
R5743	T8267	T8268	det	the,cohorts
R5744	T8407	T8398	advmod	completely,halted
R5745	T8408	T8409	det	the,progression
R5746	T8268	T8266	pobj	cohorts,from
R5747	T8409	T8398	dobj	progression,halted
R5748	T8410	T8409	prep	of,progression
R5749	T8411	T8410	pobj	pathology,of
R5750	T8269	T8270	nummod	6,mo
R5751	T8412	T8394	punct	.,was
R5752	T8270	T8271	npadvmod	mo,treated
R5753	T8414	T8415	aux	To,confirm
R5754	T8415	T8416	advcl	confirm,repeated
R5755	T8271	T8268	amod	treated,cohorts
R5756	T8417	T8418	mark	that,was
R5757	T8418	T8415	ccomp	was,confirm
R5758	T8272	T8270	punct	+,mo
R5759	T8419	T8420	det	the,arrest
R5760	T8420	T8418	nsubj	arrest,was
R5761	T8421	T8420	prep	of,arrest
R5762	T8273	T8274	nummod	3,mo
R5763	T8422	T8421	pobj	plaques,of
R5764	T8423	T8420	prep	without,arrest
R5765	T8274	T8270	appos	mo,mo
R5766	T8424	T8425	det	any,sign
R5767	T8425	T8423	pobj	sign,without
R5768	T8426	T8425	prep	of,sign
R5769	T8275	T8271	cc	or,treated
R5770	T8427	T8426	pobj	clearance,of
R5771	T8428	T8418	neg	not,was
R5772	T8276	T8277	nummod	6,mo
R5773	T8277	T8278	npadvmod	mo,treated
R5774	T8278	T8271	conj	treated,treated
R5775	T8429	T8418	acomp	unique,was
R5776	T8430	T8429	prep	to,unique
R5777	T8279	T8277	punct	+,mo
R5778	T8431	T8432	det	the,mice
R5779	T8432	T8430	pobj	mice,to
R5780	T8280	T8281	nummod	6,mo
R5781	T8433	T8432	nmod	line,mice
R5782	T8434	T8433	nummod	107,line
R5783	T8435	T8416	punct	", ",repeated
R5784	T8436	T8416	nsubj	we,repeated
R5785	T8437	T8438	det	the,experiment
R5786	T8438	T8416	dobj	experiment,repeated
R5787	T8281	T8277	appos	mo,mo
R5788	T8439	T8440	compound	dox,suppression
R5789	T8440	T8438	compound	suppression,experiment
R5790	T8282	T8256	advmod	closely,resembled
R5791	T8441	T8440	punct	-,suppression
R5792	T8442	T8416	prep	in,repeated
R5793	T8443	T8444	det	a,line
R5794	T8283	T8256	dobj	that,resembled
R5795	T8444	T8442	pobj	line,in
R5796	T8445	T8444	amod	second,line
R5797	T8446	T8444	prep	of,line
R5798	T8496	T8497	punct	(,8
R5799	T8447	T8448	compound	tet,off
R5800	T8448	T8450	compound	off,mice
R5801	T8449	T8448	punct	-,off
R5802	T8497	T8488	parataxis	8,harvested
R5803	T8498	T8499	nummod	6,mo
R5804	T8450	T8446	pobj	mice,of
R5805	T8451	T8450	compound	APP,mice
R5806	T8499	T8500	npadvmod	mo,untreated
R5807	T8452	T8453	punct	(,line
R5808	T8453	T8416	parataxis	line,repeated
R5809	T8500	T8501	amod	untreated,8
R5810	T8454	T8455	nmod	CaMKIIα,ind
R5811	T8455	T8453	compound	ind,line
R5812	T8501	T8497	dep	8,8
R5813	T8456	T8455	punct	-,ind
R5814	T8457	T8455	nmod	tTA,ind
R5815	T8458	T8455	punct	×,ind
R5816	T8502	T8501	punct	", ",8
R5817	T8459	T8455	compound	tet,ind
R5818	T8460	T8455	punct	-,ind
R5819	T8503	T8501	nsubj	n,8
R5820	T8461	T8455	compound	APPswe,ind
R5821	T8462	T8455	punct	/,ind
R5822	T8463	T8453	nummod	18,line
R5823	T8504	T8501	punct	=,8
R5824	T8464	T8453	punct	;,line
R5825	T8465	T8466	nsubj	n,22
R5826	T8505	T8497	punct	;,8
R5827	T8466	T8453	ccomp	22,line
R5828	T8467	T8466	punct	=,22
R5829	T8468	T8453	punct	),line
R5830	T8506	T8507	nummod	9,mo
R5831	T8469	T8416	punct	.,repeated
R5832	T8471	T8472	advmod	Again,begun
R5833	T8473	T8472	punct	", ",begun
R5834	T8507	T8508	npadvmod	mo,untreated
R5835	T8474	T8475	amod	long,term
R5836	T8475	T8477	compound	term,treatment
R5837	T8476	T8475	punct	-,term
R5838	T8508	T8509	amod	untreated,6
R5839	T8477	T8472	nsubjpass	treatment,begun
R5840	T8478	T8477	compound	dox,treatment
R5841	T8509	T8497	dep	6,8
R5842	T8479	T8472	auxpass	was,begun
R5843	T8480	T8472	prep	at,begun
R5844	T8481	T8482	nummod	6,mo
R5845	T8510	T8509	punct	", ",6
R5846	T8482	T8480	pobj	mo,at
R5847	T8483	T8482	prep	of,mo
R5848	T8511	T8509	nsubj	n,6
R5849	T8484	T8483	pobj	age,of
R5850	T8485	T8472	punct	", ",begun
R5851	T8486	T8472	cc	and,begun
R5852	T8512	T8509	punct	=,6
R5853	T8487	T8488	nsubjpass	mice,harvested
R5854	T8488	T8472	conj	harvested,begun
R5855	T8489	T8488	auxpass	were,harvested
R5856	T8513	T8497	punct	;,8
R5857	T8490	T8488	prep	after,harvested
R5858	T8491	T8492	nummod	3,mo
R5859	T8514	T8515	nummod	6,mo
R5860	T8492	T8490	pobj	mo,after
R5861	T8493	T8492	prep	of,mo
R5862	T8494	T8495	compound	transgene,suppression
R5863	T8515	T8519	npadvmod	mo,treated
R5864	T8495	T8493	pobj	suppression,of
R5865	T8516	T8515	nmod	mo,mo
R5866	T8517	T8515	punct	+,mo
R5867	T8518	T8515	nummod	3,mo
R5868	T8519	T8497	amod	treated,8
R5869	T8603	T8602	aux	could,found
R5870	T8520	T8497	punct	", ",8
R5871	T8604	T8602	advmod	still,found
R5872	T8605	T8602	auxpass	be,found
R5873	T8606	T8602	prep	in,found
R5874	T8521	T8497	nsubj	n,8
R5875	T8607	T8606	pobj	each,in
R5876	T8608	T8607	prep	of,each
R5877	T8522	T8497	punct	=,8
R5878	T8609	T8610	det	the,animals
R5879	T8610	T8608	pobj	animals,of
R5880	T8611	T8612	npadvmod	dox,treated
R5881	T8523	T8497	punct	),8
R5882	T8612	T8610	amod	treated,animals
R5883	T8613	T8612	punct	-,treated
R5884	T8614	T8602	punct	.,found
R5885	T8616	T8617	nsubj	We,measured
R5886	T8524	T8488	punct	.,harvested
R5887	T8618	T8617	advmod	biochemically,measured
R5888	T8619	T8620	det	the,amount
R5889	T8526	T8527	nsubj	Immunoblotting,confirmed
R5890	T8620	T8617	dobj	amount,measured
R5891	T8621	T8620	prep	of,amount
R5892	T8622	T8623	amod	aggregated,Aβ
R5893	T8623	T8621	pobj	Aβ,of
R5894	T8528	T8526	prep	for,Immunoblotting
R5895	T8624	T8617	prep	in,measured
R5896	T8529	T8528	pobj	APP,for
R5897	T8530	T8531	amod	full,suppression
R5898	T8625	T8626	det	the,brains
R5899	T8626	T8624	pobj	brains,in
R5900	T8531	T8527	dobj	suppression,confirmed
R5901	T8627	T8626	prep	of,brains
R5902	T8628	T8629	poss	our,mice
R5903	T8629	T8627	pobj	mice,of
R5904	T8532	T8531	compound	transgene,suppression
R5905	T8630	T8617	prep	before,measured
R5906	T8631	T8630	cc	and,before
R5907	T8533	T8527	prep	in,confirmed
R5908	T8632	T8630	conj	after,before
R5909	T8633	T8634	compound	transgene,suppression
R5910	T8534	T8535	det	the,animals
R5911	T8634	T8632	pobj	suppression,after
R5912	T8635	T8617	advcl	using,measured
R5913	T8636	T8637	compound	filter,trap
R5914	T8535	T8533	pobj	animals,in
R5915	T8637	T8638	compound	trap,analysis
R5916	T8638	T8635	dobj	analysis,using
R5917	T8536	T8535	amod	treated,animals
R5918	T8639	T8638	prep	of,analysis
R5919	T8640	T8641	amod	cortical,tissue
R5920	T8641	T8639	pobj	tissue,of
R5921	T8537	T8538	punct	(,S5
R5922	T8642	T8641	prep	from,tissue
R5923	T8643	T8644	det	each,animal
R5924	T8644	T8642	pobj	animal,from
R5925	T8538	T8527	parataxis	S5,confirmed
R5926	T8645	T8617	punct	.,measured
R5927	T8539	T8538	compound	Figure,S5
R5928	T8647	T8648	prep	In,passed
R5929	T8648	T8658	ccomp	passed,become
R5930	T8649	T8650	det	this,assay
R5931	T8540	T8538	punct	),S5
R5932	T8650	T8647	pobj	assay,In
R5933	T8651	T8648	punct	", ",passed
R5934	T8541	T8527	punct	.,confirmed
R5935	T8652	T8653	amod	serial,dilutions
R5936	T8653	T8648	nsubjpass	dilutions,passed
R5937	T8654	T8653	prep	of,dilutions
R5938	T8655	T8656	compound	protein,homogenate
R5939	T8656	T8654	pobj	homogenate,of
R5940	T8543	T8544	prep	As,worsened
R5941	T8657	T8648	auxpass	are,passed
R5942	T8659	T8648	prep	through,passed
R5943	T8660	T8661	det	a,filter
R5944	T8545	T8543	prep	in,As
R5945	T8661	T8659	pobj	filter,through
R5946	T8662	T8661	compound	cellulose,filter
R5947	T8663	T8661	compound	acetate,filter
R5948	T8664	T8658	punct	;,become
R5949	T8546	T8547	det	the,mice
R5950	T8665	T8658	nsubj	particles,become
R5951	T8666	T8665	amod	larger,particles
R5952	T8547	T8545	pobj	mice,in
R5953	T8667	T8666	prep	than,larger
R5954	T8668	T8669	det	the,size
R5955	T8548	T8547	nmod	line,mice
R5956	T8669	T8667	pobj	size,than
R5957	T8670	T8669	compound	pore,size
R5958	T8549	T8548	nummod	107,line
R5959	T8671	T8669	prep	of,size
R5960	T8672	T8673	det	the,filter
R5961	T8673	T8671	pobj	filter,of
R5962	T8550	T8547	acl	described,mice
R5963	T8674	T8658	acomp	trapped,become
R5964	T8675	T8674	prep	in,trapped
R5965	T8551	T8550	advmod	above,described
R5966	T8676	T8677	det	the,membrane
R5967	T8677	T8675	pobj	membrane,in
R5968	T8552	T8544	punct	", ",worsened
R5969	T8678	T8658	cc	and,become
R5970	T8679	T8680	auxpass	are,revealed
R5971	T8553	T8554	compound	amyloid,burden
R5972	T8680	T8658	conj	revealed,become
R5973	T8681	T8680	prep	by,revealed
R5974	T8682	T8681	pobj	immunoblotting,by
R5975	T8554	T8544	nsubj	burden,worsened
R5976	T8683	T8684	punct	[,22
R5977	T8684	T8680	parataxis	22,revealed
R5978	T8555	T8544	advmod	substantially,worsened
R5979	T8685	T8684	punct	],22
R5980	T8686	T8658	punct	.,become
R5981	T8556	T8544	prep	in,worsened
R5982	T8688	T8689	advcl	Consistent,had
R5983	T8557	T8558	det	the,mice
R5984	T8690	T8688	prep	with,Consistent
R5985	T8691	T8692	poss	our,analysis
R5986	T8558	T8556	pobj	mice,in
R5987	T8692	T8690	pobj	analysis,with
R5988	T8693	T8692	amod	visual,analysis
R5989	T8694	T8692	prep	of,analysis
R5990	T8695	T8696	det	the,sections
R5991	T8696	T8694	pobj	sections,of
R5992	T8697	T8696	amod	histological,sections
R5993	T8559	T8558	amod	untreated,mice
R5994	T8698	T8689	punct	", ",had
R5995	T8699	T8700	nmod	line,mice
R5996	T8560	T8558	prep	between,mice
R5997	T8700	T8689	nsubj	mice,had
R5998	T8701	T8699	nummod	107,line
R5999	T8702	T8703	compound	tTA,APP
R6000	T8561	T8562	nummod	6,mo
R6001	T8703	T8700	compound	APP,mice
R6002	T8704	T8703	punct	/,APP
R6003	T8562	T8560	pobj	mo,between
R6004	T8705	T8700	acl	treated,mice
R6005	T8706	T8705	prep	with,treated
R6006	T8707	T8706	pobj	dox,with
R6007	T8563	T8561	cc	and,6
R6008	T8564	T8561	conj	9,6
R6009	T8565	T8562	prep	of,mo
R6010	T8566	T8565	pobj	age,of
R6011	T8708	T8705	prep	for,treated
R6012	T8709	T8710	nummod	3,mo
R6013	T8567	T8544	punct	.,worsened
R6014	T8569	T8570	nsubj	Suppression,arrested
R6015	T8571	T8569	prep	of,Suppression
R6016	T8710	T8708	pobj	mo,for
R6017	T8711	T8710	cc	or,mo
R6018	T8712	T8710	nummod	6,mo
R6019	T8572	T8573	compound	transgene,expression
R6020	T8713	T8714	det	the,amount
R6021	T8714	T8689	dobj	amount,had
R6022	T8573	T8571	pobj	expression,of
R6023	T8715	T8714	amod	same,amount
R6024	T8716	T8714	prep	of,amount
R6025	T8574	T8570	advmod	abruptly,arrested
R6026	T8717	T8718	amod	aggregated,Aβ
R6027	T8718	T8716	pobj	Aβ,of
R6028	T8575	T8570	dobj	progression,arrested
R6029	T8719	T8714	prep	as,amount
R6030	T8720	T8721	advmod	when,started
R6031	T8576	T8575	prep	of,progression
R6032	T8721	T8719	pcomp	started,as
R6033	T8722	T8721	nsubj	they,started
R6034	T8723	T8721	dobj	treatment,started
R6035	T8577	T8576	pobj	pathology,of
R6036	T8724	T8721	prep	at,started
R6037	T8725	T8726	nummod	6,mo
R6038	T8578	T8579	punct	(,S6
R6039	T8726	T8724	pobj	mo,at
R6040	T8727	T8726	prep	of,mo
R6041	T8728	T8727	pobj	age,of
R6042	T8729	T8730	punct	(,4A
R6043	T8730	T8689	parataxis	4A,had
R6044	T8579	T8570	parataxis	S6,arrested
R6045	T8731	T8730	compound	Figure,4A
R6046	T8732	T8730	cc	and,4A
R6047	T8733	T8730	conj	4B,4A
R6048	T8580	T8579	compound	Figure,S6
R6049	T8734	T8730	punct	),4A
R6050	T8735	T8689	punct	.,had
R6051	T8581	T8579	punct	),S6
R6052	T8737	T8738	prep	In,had
R6053	T8582	T8570	punct	", ",arrested
R6054	T8739	T8737	pobj	contrast,In
R6055	T8740	T8738	punct	", ",had
R6056	T8583	T8584	cc	but,without
R6057	T8741	T8742	amod	untreated,mice
R6058	T8742	T8738	nsubj	mice,had
R6059	T8584	T8570	prep	without,arrested
R6060	T8743	T8744	nummod	9,mo
R6061	T8744	T8746	npadvmod	mo,old
R6062	T8745	T8744	punct	-,mo
R6063	T8585	T8584	advmod	again,without
R6064	T8746	T8742	amod	old,mice
R6065	T8747	T8746	punct	-,old
R6066	T8586	T8587	det	any,sign
R6067	T8748	T8749	advmod	almost,twice
R6068	T8749	T8750	advmod	twice,much
R6069	T8587	T8584	pobj	sign,without
R6070	T8750	T8752	amod	much,Aβ
R6071	T8751	T8750	advmod	as,much
R6072	T8588	T8587	prep	of,sign
R6073	T8752	T8738	dobj	Aβ,had
R6075	T8754	T8752	prep	as,Aβ
R6076	T8589	T8588	pobj	reduction,of
R6079	T8590	T8570	punct	.,arrested
R6080	T8757	T8758	det	the,groups
R6082	T8759	T8758	amod	treated,groups
R6083	T8592	T8593	preconj	Both,silver
R6084	T8760	T8738	punct	.,had
R6085	T8762	T8763	compound	Filter,analysis
R6086	T8593	T8594	npadvmod	silver,positive
R6087	T8763	T8765	nsubj	analysis,yielded
R6088	T8764	T8763	compound	trap,analysis
R6089	T8765	T8773	ccomp	yielded,arrested
R6090	T8594	T8601	amod	positive,plaques
R6091	T8766	T8763	prep	of,analysis
R6092	T8767	T8768	nmod	line,mice
R6093	T8768	T8766	pobj	mice,of
R6094	T8769	T8767	nummod	18,line
R6095	T8595	T8593	punct	-,silver
R6096	T8770	T8771	compound	tTA,APP
R6097	T8771	T8768	compound	APP,mice
R6098	T8772	T8771	punct	/,APP
R6099	T8596	T8593	cc	and,silver
R6100	T8774	T8775	amod	similar,results
R6101	T8597	T8598	compound	thioflavin,S
R6102	T8775	T8765	dobj	results,yielded
R6103	T8776	T8773	punct	: ,arrested
R6104	T8598	T8593	conj	S,silver
R6105	T8777	T8778	det	the,increase
R6106	T8778	T8773	nsubjpass	increase,arrested
R6107	T8599	T8598	punct	-,S
R6108	T8779	T8778	prep	in,increase
R6109	T8780	T8781	amod	aggregated,Aβ
R6110	T8781	T8779	pobj	Aβ,in
R6111	T8600	T8594	punct	-,positive
R6112	T8782	T8778	acl	observed,increase
R6113	T8783	T8782	prep	in,observed
R6114	T8601	T8602	nsubjpass	plaques,found
R6115	T8784	T8785	amod	untreated,animals
R6116	T8785	T8783	pobj	animals,in
R6117	T8786	T8785	prep	between,animals
R6118	T8814	T8811	prep	in,measure
R6119	T8787	T8788	nummod	6,mo
R6120	T8788	T8786	pobj	mo,between
R6121	T8789	T8787	cc	and,6
R6122	T8790	T8787	conj	9,6
R6123	T8815	T8816	det	the,brains
R6124	T8791	T8788	prep	of,mo
R6125	T8792	T8791	pobj	age,of
R6126	T8793	T8773	auxpass	was,arrested
R6127	T8816	T8814	pobj	brains,in
R6128	T8794	T8773	advmod	completely,arrested
R6129	T8817	T8816	prep	of,brains
R6130	T8818	T8819	det	each,group
R6131	T8795	T8773	agent	by,arrested
R6132	T8796	T8797	compound	transgene,suppression
R6133	T8819	T8817	pobj	group,of
R6134	T8797	T8795	pobj	suppression,by
R6135	T8798	T8799	punct	(,S5C
R6136	T8799	T8773	parataxis	S5C,arrested
R6137	T8820	T8821	aux	to,determine
R6138	T8800	T8799	compound	Figure,S5C
R6139	T8801	T8799	cc	and,S5C
R6140	T8821	T8811	advcl	determine,measure
R6141	T8802	T8799	conj	S5D,S5C
R6142	T8803	T8799	punct	),S5C
R6143	T8822	T8823	mark	whether,occurred
R6144	T8804	T8773	punct	.,arrested
R6145	T8806	T8807	nsubj	We,used
R6146	T8808	T8807	advmod	next,used
R6147	T8823	T8821	ccomp	occurred,determine
R6148	T8809	T8807	dobj	ELISA,used
R6149	T8810	T8811	aux	to,measure
R6150	T8824	T8825	det	any,change
R6151	T8811	T8807	advcl	measure,used
R6152	T8812	T8813	amod	total,Aβ
R6153	T8813	T8811	dobj	Aβ,measure
R6154	T8825	T8823	nsubj	change,occurred
R6155	T8826	T8825	prep	in,change
R6156	T8827	T8828	det	the,amount
R6157	T8828	T8826	pobj	amount,in
R6158	T8829	T8828	cc	or,amount
R6159	T8830	T8828	conj	solubility,amount
R6160	T8920	T8918	prep	with,Consistent
R6161	T8921	T8922	det	the,results
R6162	T8831	T8828	prep	of,amount
R6163	T8922	T8920	pobj	results,with
R6164	T8923	T8922	compound	filter,results
R6165	T8832	T8831	pobj	peptide,of
R6166	T8924	T8922	compound	trap,results
R6167	T8925	T8922	acl	presented,results
R6168	T8926	T8925	advmod	above,presented
R6169	T8833	T8834	mark	while,suppressed
R6170	T8927	T8919	punct	", ",were
R6171	T8928	T8919	expl	there,were
R6172	T8834	T8823	advcl	suppressed,occurred
R6173	T8929	T8930	det	no,differences
R6174	T8930	T8919	attr	differences,were
R6175	T8931	T8930	amod	significant,differences
R6176	T8835	T8836	compound	APPswe,ind
R6177	T8932	T8930	prep	in,differences
R6178	T8933	T8934	npadvmod	SDS,soluble
R6179	T8836	T8838	compound	ind,expression
R6180	T8934	T8939	amod	soluble,Aβ
R6181	T8935	T8933	punct	-,SDS
R6182	T8936	T8933	cc	or,SDS
R6183	T8837	T8836	punct	/,ind
R6184	T8937	T8933	conj	FA,SDS
R6185	T8938	T8934	punct	-,soluble
R6186	T8939	T8932	pobj	Aβ,in
R6187	T8838	T8834	nsubjpass	expression,suppressed
R6188	T8940	T8930	prep	between,differences
R6189	T8941	T8942	det	the,cohort
R6190	T8839	T8834	auxpass	was,suppressed
R6191	T8942	T8940	pobj	cohort,between
R6192	T8943	T8944	nummod	6,mo
R6193	T8944	T8942	nmod	mo,cohort
R6194	T8840	T8807	punct	.,used
R6195	T8945	T8942	amod	untreated,cohort
R6196	T8946	T8942	cc	and,cohort
R6197	T8947	T8948	preconj	either,cohorts
R6198	T8948	T8942	conj	cohorts,cohort
R6199	T8949	T8948	det	the,cohorts
R6200	T8842	T8843	amod	Cortical,homogenates
R6201	T8950	T8951	nummod	6,mo
R6202	T8951	T8948	nmod	mo,cohorts
R6203	T8952	T8951	punct	+,mo
R6204	T8843	T8844	nsubjpass	homogenates,extracted
R6205	T8953	T8954	nummod	3,mo
R6206	T8954	T8951	appos	mo,mo
R6207	T8955	T8954	acl	treated,mo
R6208	T8844	T8847	ccomp	extracted,measured
R6209	T8956	T8951	cc	or,mo
R6210	T8957	T8958	nummod	6,mo
R6211	T8958	T8951	conj	mo,mo
R6212	T8845	T8844	auxpass	were,extracted
R6213	T8959	T8958	punct	+,mo
R6214	T8960	T8961	nummod	6,mo
R6215	T8846	T8844	advmod	sequentially,extracted
R6216	T8961	T8958	appos	mo,mo
R6217	T8962	T8961	acl	treated,mo
R6218	T8963	T8919	punct	.,were
R6219	T8848	T8849	aux	to,separate
R6220	T8965	T8966	advmod	However,contained
R6221	T8849	T8844	advcl	separate,extracted
R6222	T8850	T8849	dobj	peptide,separate
R6223	T8967	T8966	punct	", ",contained
R6224	T8968	T8966	nsubj	brains,contained
R6225	T8851	T8849	prep	into,separate
R6226	T8969	T8968	prep	of,brains
R6227	T8970	T8971	preconj	both,mo
R6228	T8971	T8973	npadvmod	mo,treated
R6229	T8852	T8853	npadvmod	PBS,soluble
R6230	T8972	T8971	nummod	6,mo
R6231	T8973	T8983	amod	treated,cohorts
R6232	T8853	T8862	amod	soluble,fractions
R6233	T8974	T8971	punct	+,mo
R6234	T8975	T8976	nummod	3,mo
R6235	T8854	T8852	punct	-,PBS
R6236	T8976	T8971	appos	mo,mo
R6237	T8977	T8971	cc	and,mo
R6238	T8978	T8979	nummod	6,mo
R6239	T8855	T8852	punct	", ",PBS
R6240	T8979	T8971	conj	mo,mo
R6241	T8980	T8979	punct	+,mo
R6242	T8981	T8982	nummod	6,mo
R6243	T8856	T8852	conj	SDS,PBS
R6244	T8982	T8979	appos	mo,mo
R6245	T8983	T8969	pobj	cohorts,of
R6246	T8857	T8856	punct	-,SDS
R6247	T8984	T8985	advmod	roughly,twice
R6248	T8985	T8986	advmod	twice,much
R6249	T8986	T8988	amod	much,Aβ40
R6250	T8987	T8986	advmod	as,much
R6251	T8988	T8966	dobj	Aβ40,contained
R6252	T8858	T8856	punct	", ",SDS
R6253	T8989	T8988	nmod	PBS,Aβ40
R6254	T8990	T8988	punct	-,Aβ40
R6255	T8991	T8988	amod	soluble,Aβ40
R6256	T8859	T8856	cc	and,SDS
R6257	T8992	T8988	prep	as,Aβ40
R6258	T8993	T8994	amod	untreated,mice
R6259	T8860	T8856	conj	FA,SDS
R6260	T8994	T8992	pobj	mice,as
R6261	T8995	T8996	nummod	6,mo
R6262	T8996	T8998	npadvmod	mo,old
R6263	T8861	T8853	punct	-,soluble
R6264	T8997	T8996	punct	-,mo
R6265	T8998	T8994	amod	old,mice
R6266	T8862	T8851	pobj	fractions,into
R6267	T8999	T8998	punct	-,old
R6268	T9000	T9001	punct	(,5C
R6269	T8863	T8847	punct	", ",measured
R6270	T9001	T8966	parataxis	5C,contained
R6271	T9002	T9001	compound	Figure,5C
R6272	T9003	T9001	punct	),5C
R6273	T8864	T8847	advmod	then,measured
R6274	T9004	T8966	punct	.,contained
R6275	T8865	T8866	npadvmod	transgene,derived
R6276	T9006	T9007	nsubj	Levels,showed
R6277	T9008	T9006	prep	of,Levels
R6278	T8866	T8868	amod	derived,Aβ40
R6279	T9009	T9008	pobj	Aβ42,of
R6280	T9010	T9011	det	a,trend
R6281	T8867	T8866	punct	-,derived
R6282	T9011	T9007	dobj	trend,showed
R6283	T9012	T9011	amod	similar,trend
R6284	T9013	T9007	punct	", ",showed
R6285	T8868	T8847	nsubjpass	Aβ40,measured
R6286	T9014	T9007	cc	but,showed
R6287	T9015	T9016	aux	did,reach
R6288	T8869	T8868	cc	and,Aβ40
R6289	T9016	T9007	conj	reach,showed
R6290	T9017	T9016	neg	not,reach
R6291	T9018	T9019	amod	statistical,significance
R6292	T8870	T8868	conj	Aβ42,Aβ40
R6293	T9019	T9016	dobj	significance,reach
R6294	T9020	T9007	punct	.,showed
R6295	T8871	T8847	auxpass	were,measured
R6296	T9022	T9023	prep	In,were
R6297	T8872	T8847	agent	by,measured
R6298	T9024	T9022	pobj	fact,In
R6299	T8873	T8874	amod	human,specific
R6300	T9025	T9023	punct	", ",were
R6301	T9026	T9023	nsubj	levels,were
R6302	T8874	T8876	amod	specific,ELISA
R6303	T9027	T9026	prep	of,levels
R6304	T9028	T9029	npadvmod	PBS,soluble
R6305	T9029	T9031	amod	soluble,Aβ40
R6306	T8875	T8874	punct	-,specific
R6307	T9030	T9029	punct	-,soluble
R6308	T9031	T9027	pobj	Aβ40,of
R6309	T8876	T8872	pobj	ELISA,by
R6310	T9032	T9031	cc	and,Aβ40
R6311	T9033	T9031	conj	Aβ42,Aβ40
R6312	T9034	T9026	prep	in,levels
R6313	T8877	T8878	punct	[,23
R6314	T9035	T9036	det	the,cohorts
R6315	T9036	T9034	pobj	cohorts,in
R6316	T8878	T8847	parataxis	23,measured
R6317	T9037	T9038	nummod	6,mo
R6318	T9038	T9036	nmod	mo,cohorts
R6319	T9039	T9038	punct	+,mo
R6320	T8879	T8878	punct	],23
R6321	T9040	T9041	nummod	3,mo
R6322	T9041	T9038	appos	mo,mo
R6323	T8880	T8847	punct	.,measured
R6324	T9042	T9038	cc	and,mo
R6325	T9043	T9044	nummod	6,mo
R6326	T9044	T9038	conj	mo,mo
R6327	T8882	T8883	prep	In,found
R6328	T9045	T9044	punct	+,mo
R6329	T9046	T9047	nummod	6,mo
R6330	T9047	T9044	appos	mo,mo
R6331	T9048	T9044	acl	treated,mo
R6332	T8884	T8885	det	all,animals
R6333	T9049	T9050	advmod	most,similar
R6334	T9050	T9023	acomp	similar,were
R6335	T9051	T9050	prep	to,similar
R6336	T8885	T8882	pobj	animals,In
R6337	T8886	T8885	acl	harboring,animals
R6338	T9052	T9051	pobj	that,to
R6339	T9053	T9052	prep	of,that
R6340	T8887	T8888	compound	amyloid,deposits
R6341	T9054	T9055	det	the,cohort
R6342	T9055	T9053	pobj	cohort,of
R6343	T9056	T9057	nummod	9,mo
R6344	T8888	T8886	dobj	deposits,harboring
R6345	T9057	T9055	nmod	mo,cohort
R6346	T9058	T9055	amod	untreated,cohort
R6347	T8889	T8883	punct	", ",found
R6348	T9059	T9023	punct	", ",were
R6349	T9060	T9023	advcl	suggesting,were
R6350	T8890	T8883	nsubj	we,found
R6351	T9061	T9062	mark	that,determine
R6352	T9062	T9060	ccomp	determine,suggesting
R6353	T9063	T9062	nsubj	age,determine
R6354	T9064	T9063	punct	", ",age
R6355	T9065	T9063	prep	as,age
R6356	T9066	T9065	prep	opposed,as
R6357	T8891	T8892	mark	that,extracted
R6358	T9067	T9066	prep	to,opposed
R6359	T9068	T9069	amod	synthetic,rate
R6360	T8892	T8883	ccomp	extracted,found
R6361	T9069	T9067	pobj	rate,to
R6362	T9070	T9069	punct	(,rate
R6363	T9071	T9072	dep	which,be
R6364	T8893	T8894	det	the,majority
R6365	T9072	T9069	relcl	be,rate
R6366	T9073	T9072	aux	would,be
R6367	T8894	T8892	nsubjpass	majority,extracted
R6368	T9074	T9072	acomp	negligible,be
R6369	T9075	T9072	prep	in,be
R6370	T9076	T9077	det	the,animals
R6371	T8895	T8894	amod	vast,majority
R6372	T9077	T9075	pobj	animals,in
R6373	T9078	T9077	amod	treated,animals
R6374	T9079	T9062	punct	),determine
R6375	T8896	T8894	prep	of,majority
R6376	T9080	T9062	punct	", ",determine
R6377	T9081	T9062	aux	may,determine
R6378	T9082	T9083	det	the,fraction
R6379	T8897	T8896	pobj	Aβ,of
R6380	T9083	T9062	dobj	fraction,determine
R6381	T9084	T9083	prep	of,fraction
R6382	T8898	T8899	punct	(,%
R6383	T9085	T9086	npadvmod	PBS,soluble
R6384	T9086	T9088	amod	soluble,Aβ
R6385	T9087	T9086	punct	-,soluble
R6386	T8899	T8897	parataxis	%,Aβ
R6387	T9088	T9084	pobj	Aβ,of
R6388	T9089	T9062	prep	in,determine
R6389	T9090	T9091	det	these,animals
R6390	T8900	T8901	punct	>,99
R6391	T9091	T9089	pobj	animals,in
R6392	T9092	T9023	punct	.,were
R6393	T8901	T8899	nummod	99,%
R6394	T9094	T9095	nsubj	We,assessed
R6395	T8902	T8899	punct	),%
R6396	T9096	T9095	advmod	also,assessed
R6397	T9097	T9098	amod	neuritic,pathology
R6398	T8903	T8892	auxpass	was,extracted
R6399	T9098	T9095	dobj	pathology,assessed
R6400	T9099	T9097	cc	and,neuritic
R6401	T9100	T9097	conj	glial,neuritic
R6402	T8904	T8892	prep	into,extracted
R6403	T9101	T9098	acl	surrounding,pathology
R6404	T9102	T9103	det	the,plaques
R6405	T9103	T9101	dobj	plaques,surrounding
R6406	T9104	T9105	aux	to,determine
R6407	T9105	T9095	advcl	determine,assessed
R6408	T8905	T8906	det	the,fractions
R6409	T9106	T9107	mark	whether,were
R6410	T9107	T9105	ccomp	were,determine
R6411	T8906	T8904	pobj	fractions,into
R6412	T9108	T9107	expl	there,were
R6415	T8907	T8906	nmod	SDS,fractions
R6416	T9111	T9110	prep	in,changes
R6417	T9112	T9113	amod	nearby,tissue
R6418	T8908	T8907	cc	and,SDS
R6419	T9113	T9111	pobj	tissue,in
R6420	T9114	T9107	prep	following,were
R6421	T9115	T9116	amod	long,term
R6422	T8909	T8907	conj	FA,SDS
R6423	T9116	T9118	compound	term,suppression
R6424	T9117	T9116	punct	-,term
R6425	T8910	T8911	punct	(,5A
R6426	T9118	T9114	pobj	suppression,following
R6427	T9119	T9118	compound	transgene,suppression
R6428	T9120	T9095	punct	.,assessed
R6429	T8911	T8892	parataxis	5A,extracted
R6430	T9122	T9123	preconj	Both,stain
R6431	T8912	T8911	compound	Figure,5A
R6432	T9123	T9126	nsubj	stain,showed
R6433	T9124	T9123	compound	Hirano,stain
R6434	T9125	T9123	compound	silver,stain
R6435	T8913	T8911	cc	and,5A
R6436	T9127	T9123	cc	and,stain
R6437	T9128	T9129	compound	ubiquitin,immunostaining
R6438	T8914	T8911	conj	5B,5A
R6439	T9129	T9123	conj	immunostaining,stain
R6440	T9130	T9131	amod	neuritic,pathology
R6441	T8915	T8911	punct	),5A
R6442	T8916	T8883	punct	.,found
R6443	T8918	T8919	advcl	Consistent,were
R6444	T9131	T9126	dobj	pathology,showed
R6445	T9132	T9126	prep	in,showed
R6446	T9237	T9234	auxpass	was,interrupted
R6447	T9133	T9134	det	all,groups
R6448	T9134	T9132	pobj	groups,in
R6449	T9135	T9134	compound	treatment,groups
R6450	T9238	T9210	punct	.,was
R6451	T9136	T9137	punct	(,Figure
R6452	T9240	T9241	advmod	Unfortunately,compromised
R6453	T9137	T9126	parataxis	Figure,showed
R6454	T9138	T9137	nummod	6,Figure
R6455	T9139	T9137	punct	),Figure
R6456	T9140	T9126	punct	.,showed
R6457	T9242	T9241	punct	", ",compromised
R6458	T9142	T9143	advmod	Similarly,found
R6459	T9144	T9143	punct	", ",found
R6460	T9145	T9146	amod	activated,astrocytes
R6461	T9243	T9241	nsubjpass	efforts,compromised
R6462	T9146	T9143	nsubjpass	astrocytes,found
R6463	T9147	T9146	acl	immunostained,astrocytes
R6464	T9244	T9245	aux	to,characterize
R6465	T9148	T9147	prep	for,immunostained
R6466	T9149	T9148	pobj	GFAP,for
R6467	T9245	T9243	acl	characterize,efforts
R6468	T9150	T9143	auxpass	were,found
R6469	T9151	T9143	prep	near,found
R6470	T9152	T9151	pobj	plaques,near
R6471	T9246	T9247	amod	cognitive,behavior
R6472	T9153	T9143	prep	in,found
R6473	T9154	T9155	det	all,animals
R6474	T9247	T9245	dobj	behavior,characterize
R6475	T9155	T9153	pobj	animals,in
R6476	T9156	T9157	punct	(,Figure
R6477	T9248	T9241	auxpass	were,compromised
R6478	T9157	T9143	parataxis	Figure,found
R6479	T9158	T9157	nummod	6,Figure
R6480	T9159	T9157	punct	),Figure
R6481	T9249	T9241	agent	by,compromised
R6482	T9160	T9143	punct	.,found
R6483	T9162	T9163	amod	Neuritic,pathology
R6484	T9250	T9251	amod	severe,hyperactivity
R6485	T9163	T9166	nsubj	pathology,were
R6486	T9164	T9162	cc	and,Neuritic
R6487	T9251	T9249	pobj	hyperactivity,by
R6488	T9165	T9162	conj	glial,Neuritic
R6489	T9167	T9168	advmod	more,severe
R6490	T9252	T9251	prep	in,hyperactivity
R6491	T9168	T9166	acomp	severe,were
R6492	T9169	T9166	prep	in,were
R6493	T9253	T9254	amod	untreated,mice
R6494	T9170	T9171	det	the,mice
R6495	T9171	T9169	pobj	mice,in
R6496	T9172	T9171	amod	older,mice
R6497	T9254	T9252	pobj	mice,in
R6498	T9173	T9171	amod	untreated,mice
R6499	T9174	T9166	punct	.,were
R6500	T9255	T9256	amod	double,transgenic
R6501	T9176	T9177	prep	In,prevented
R6502	T9256	T9254	amod	transgenic,mice
R6503	T9178	T9176	pobj	contrast,In
R6504	T9179	T9177	punct	", ",prevented
R6505	T9180	T9181	compound	transgene,suppression
R6506	T9257	T9241	punct	.,compromised
R6507	T9181	T9177	nsubj	suppression,prevented
R6508	T9182	T9183	det	the,growth
R6509	T9183	T9177	dobj	growth,prevented
R6510	T9184	T9183	prep	of,growth
R6511	T9185	T9186	amod	individual,deposits
R6512	T9259	T9260	det	The,animals
R6513	T9186	T9184	pobj	deposits,of
R6514	T9187	T9186	relcl	apparent,deposits
R6515	T9188	T9187	prep	in,apparent
R6516	T9189	T9190	amod	untreated,mice
R6517	T9260	T9264	nsubjpass	animals,seen
R6518	T9190	T9188	pobj	mice,in
R6519	T9191	T9177	punct	", ",prevented
R6520	T9192	T9177	cc	and,prevented
R6521	T9261	T9262	compound	tTA,APP
R6522	T9193	T9177	conj	limited,prevented
R6523	T9194	T9195	det	the,pathology
R6524	T9262	T9260	compound	APP,animals
R6525	T9195	T9193	dobj	pathology,limited
R6526	T9196	T9195	amod	surrounding,pathology
R6527	T9197	T9193	prep	to,limited
R6528	T9263	T9262	punct	/,APP
R6529	T9198	T9199	dep	what,was
R6530	T9199	T9197	pcomp	was,to
R6531	T9265	T9264	auxpass	were,seen
R6532	T9200	T9199	advmod	already,was
R6533	T9201	T9199	acomp	present,was
R6534	T9202	T9203	advmod	when,began
R6535	T9203	T9199	advcl	began,was
R6536	T9266	T9264	advmod	often,seen
R6537	T9204	T9203	nsubj	treatment,began
R6538	T9267	T9264	advcl	running,seen
R6539	T9268	T9267	prep	in,running
R6540	T9205	T9177	punct	.,prevented
R6541	T9269	T9268	pobj	circles,in
R6542	T9207	T9208	det	An,question
R6543	T9270	T9267	prep	around,running
R6544	T9208	T9210	nsubj	question,was
R6545	T9209	T9208	amod	obvious,question
R6546	T9271	T9272	det	the,perimeter
R6547	T9211	T9212	nsubj	we,sought
R6548	T9212	T9208	advcl	sought,question
R6549	T9272	T9270	pobj	perimeter,around
R6550	T9213	T9214	aux	to,address
R6551	T9214	T9212	xcomp	address,sought
R6552	T9215	T9216	mark	whether,diminished
R6553	T9273	T9272	prep	of,perimeter
R6554	T9216	T9210	attr	diminished,was
R6555	T9217	T9218	det	the,deposition
R6556	T9218	T9216	nsubj	deposition,diminished
R6557	T9274	T9275	poss	their,cages
R6558	T9219	T9218	prep	of,deposition
R6559	T9220	T9219	pobj	Aβ,of
R6560	T9221	T9222	amod	cognitive,ability
R6561	T9222	T9216	dobj	ability,diminished
R6562	T9223	T9222	prep	in,ability
R6563	T9275	T9273	pobj	cages,of
R6564	T9224	T9225	amod	untreated,mice
R6565	T9225	T9223	pobj	mice,in
R6568	T9226	T9216	punct	", ",diminished
R6569	T9227	T9216	cc	and,diminished
R6570	T9278	T9279	det	a,pattern
R6571	T9228	T9229	dep	what,happen
R6572	T9229	T9216	conj	happen,diminished
R6573	T9279	T9282	nsubjpass	pattern,noted
R6574	T9230	T9229	aux	might,happen
R6575	T9231	T9229	prep	to,happen
R6576	T9232	T9231	pobj	cognition,to
R6577	T9280	T9279	amod	similar,pattern
R6578	T9233	T9234	advmod	when,interrupted
R6579	T9281	T9279	compound	swimming,pattern
R6580	T9234	T9229	advcl	interrupted,happen
R6581	T9235	T9236	det	the,process
R6582	T9236	T9234	nsubjpass	process,interrupted
R6583	T9282	T9264	conj	noted,seen
R6584	T9283	T9282	auxpass	was,noted
R6585	T9284	T9285	advmod	when,tested
R6586	T9285	T9282	advcl	tested,noted
R6587	T9343	T9344	amod	certain,criteria
R6588	T9286	T9287	det	the,mice
R6589	T9344	T9342	dobj	criteria,meet
R6590	T9345	T9344	compound	performance,criteria
R6591	T9346	T9347	punct	[,30
R6592	T9287	T9285	nsubjpass	mice,tested
R6593	T9347	T9319	parataxis	30,dealt
R6594	T9348	T9347	punct	],30
R6595	T9288	T9285	auxpass	were,tested
R6596	T9349	T9319	punct	.,dealt
R6597	T9289	T9285	prep	in,tested
R6598	T9351	T9352	prep	In,was
R6599	T9290	T9291	det	the,maze
R6600	T9353	T9354	poss	our,case
R6601	T9354	T9351	pobj	case,In
R6602	T9291	T9289	pobj	maze,in
R6603	T9355	T9352	punct	", ",was
R6604	T9356	T9357	det	the,penetrance
R6605	T9357	T9352	nsubj	penetrance,was
R6606	T9292	T9291	compound	Morris,maze
R6607	T9358	T9357	prep	of,penetrance
R6608	T9359	T9358	pobj	hyperactivity,of
R6609	T9293	T9291	compound	water,maze
R6610	T9360	T9361	amod	close,100
R6611	T9361	T9363	nummod	100,%
R6612	T9362	T9361	quantmod	to,100
R6613	T9363	T9352	attr	%,was
R6614	T9364	T9352	punct	", ",was
R6615	T9294	T9282	punct	.,noted
R6616	T9365	T9352	advcl	leaving,was
R6617	T9366	T9365	dobj	us,leaving
R6618	T9367	T9365	prep	with,leaving
R6619	T9296	T9297	prep	In,noted
R6620	T9368	T9369	det	no,animals
R6621	T9369	T9367	pobj	animals,with
R6622	T9370	T9369	amod	testable,animals
R6623	T9298	T9299	det	the,maze
R6624	T9371	T9352	punct	.,was
R6625	T9373	T9374	det	This,phenotype
R6626	T9374	T9375	nsubj	phenotype,affected
R6627	T9299	T9296	pobj	maze,In
R6628	T9300	T9299	amod	radial,maze
R6629	T9376	T9375	aux	has,affected
R6630	T9377	T9375	neg	not,affected
R6631	T9378	T9379	amod	previous,lines
R6632	T9301	T9299	compound	water,maze
R6633	T9379	T9375	dobj	lines,affected
R6634	T9380	T9379	prep	of,lines
R6635	T9302	T9297	punct	", ",noted
R6636	T9381	T9382	compound	APPswe,mice
R6637	T9382	T9380	pobj	mice,of
R6638	T9303	T9304	amod	repetitive,patterns
R6639	T9383	T9384	nsubj	we,produced
R6640	T9384	T9379	advcl	produced,lines
R6641	T9304	T9297	nsubjpass	patterns,noted
R6642	T9385	T9384	aux	have,produced
R6643	T9386	T9379	punct	", ",lines
R6644	T9387	T9388	amod	such,as
R6645	T9305	T9304	compound	swim,patterns
R6646	T9388	T9379	prep	as,lines
R6647	T9389	T9390	compound	lines,E1
R6648	T9306	T9297	auxpass	were,noted
R6649	T9390	T9388	pobj	E1,as
R6650	T9391	T9390	punct	–,E1
R6651	T9392	T9390	nummod	2,E1
R6652	T9307	T9297	prep	with,noted
R6653	T9393	T9390	cc	or,E1
R6654	T9394	T9390	conj	C3,E1
R6655	T9308	T9309	det	no,evidence
R6656	T9395	T9394	punct	–,C3
R6657	T9396	T9394	nummod	3,C3
R6658	T9309	T9307	pobj	evidence,with
R6659	T9397	T9379	punct	", ",lines
R6660	T9398	T9399	dep	that,express
R6661	T9399	T9379	relcl	express,lines
R6662	T9310	T9309	prep	of,evidence
R6663	T9311	T9312	npadvmod	choice,motivated
R6664	T9400	T9401	amod	lower,levels
R6665	T9401	T9399	dobj	levels,express
R6666	T9402	T9401	prep	of,levels
R6667	T9312	T9314	amod	motivated,actions
R6668	T9403	T9404	amod	transgenic,protein
R6669	T9404	T9402	pobj	protein,of
R6670	T9313	T9312	punct	-,motivated
R6671	T9405	T9375	punct	.,affected
R6672	T9314	T9310	pobj	actions,of
R6673	T9407	T9408	advmod	Indeed,established
R6674	T9409	T9408	punct	", ",established
R6675	T9315	T9297	punct	.,noted
R6676	T9410	T9408	prep	in,established
R6677	T9411	T9412	amod	past,studies
R6678	T9317	T9318	amod	Other,studies
R6679	T9412	T9410	pobj	studies,in
R6680	T9413	T9414	advmod	where,was
R6681	T9414	T9412	relcl	was,studies
R6682	T9318	T9319	nsubj	studies,dealt
R6683	T9415	T9414	nsubj	hyperactivity,was
R6684	T9416	T9414	neg	not,was
R6685	T9417	T9418	det	a,factor
R6686	T9320	T9319	aux	have,dealt
R6687	T9418	T9414	attr	factor,was
R6688	T9419	T9408	punct	", ",established
R6689	T9420	T9408	nsubj	we,established
R6690	T9421	T9422	det	a,relationship
R6691	T9321	T9319	prep	with,dealt
R6692	T9422	T9408	dobj	relationship,established
R6693	T9423	T9422	amod	clear,relationship
R6694	T9322	T9323	amod	similar,problems
R6695	T9424	T9422	prep	between,relationship
R6696	T9425	T9426	compound	amyloid,load
R6697	T9426	T9424	pobj	load,between
R6698	T9323	T9321	pobj	problems,with
R6699	T9427	T9426	cc	and,load
R6700	T9428	T9429	amod	cognitive,ability
R6701	T9324	T9319	prep	by,dealt
R6702	T9429	T9426	conj	ability,load
R6703	T9430	T9431	punct	[,31
R6704	T9431	T9408	parataxis	31,established
R6705	T9325	T9324	pcomp	excluding,by
R6706	T9432	T9431	punct	],31
R6707	T9433	T9408	punct	.,established
R6708	T9326	T9325	dobj	animals,excluding
R6709	T9435	T9436	advmod	However,feel
R6710	T9327	T9328	dep	that,show
R6711	T9437	T9436	punct	", ",feel
R6712	T9438	T9436	prep	in,feel
R6713	T9439	T9440	det	the,study
R6714	T9328	T9326	relcl	show,animals
R6715	T9440	T9438	pobj	study,in
R6716	T9441	T9440	amod	current,study
R6717	T9442	T9436	punct	", ",feel
R6718	T9443	T9436	nsubj	we,feel
R6719	T9444	T9445	mark	that,made
R6720	T9329	T9328	aux	do,show
R6721	T9445	T9436	ccomp	made,feel
R6722	T9446	T9447	mark	although,scored
R6723	T9447	T9445	advcl	scored,made
R6724	T9330	T9328	neg	not,show
R6725	T9448	T9449	det	the,performance
R6726	T9331	T9332	amod	adequate,attention
R6727	T9332	T9328	dobj	attention,show
R6728	T9449	T9447	nsubjpass	performance,scored
R6729	T9333	T9332	prep	to,attention
R6730	T9450	T9449	amod	poor,performance
R6731	T9451	T9449	prep	of,performance
R6732	T9334	T9335	det	the,task
R6733	T9452	T9453	det	the,mice
R6734	T9453	T9451	pobj	mice,of
R6735	T9454	T9455	compound	tTA,APP
R6736	T9335	T9333	pobj	task,to
R6737	T9455	T9453	compound	APP,mice
R6738	T9456	T9455	punct	/,APP
R6739	T9336	T9328	punct	", ",show
R6740	T9457	T9449	prep	in,performance
R6741	T9458	T9459	det	the,tests
R6742	T9459	T9457	pobj	tests,in
R6743	T9337	T9328	advcl	retaining,show
R6744	T9460	T9459	compound	maze,tests
R6745	T9461	T9447	aux	could,scored
R6746	T9462	T9447	advmod	technically,scored
R6747	T9338	T9339	advmod	only,mice
R6748	T9463	T9447	auxpass	be,scored
R6749	T9464	T9447	prep	as,scored
R6750	T9339	T9337	dobj	mice,retaining
R6751	T9465	T9466	amod	cognitive,impairment
R6752	T9466	T9464	pobj	impairment,as
R6753	T9467	T9445	punct	", ",made
R6754	T9340	T9339	det	those,mice
R6755	T9468	T9469	det	the,animals
R6756	T9469	T9470	poss	animals,hyperactivity
R6757	T9341	T9342	dep	that,meet
R6758	T9470	T9445	nsubj	hyperactivity,made
R6759	T9471	T9469	case	',animals
R6760	T9472	T9470	amod	severe,hyperactivity
R6761	T9342	T9339	relcl	meet,mice
R6762	T9473	T9474	nsubj	interpretation,impossible
R6763	T9474	T9445	ccomp	impossible,made
R6764	T9475	T9473	prep	of,interpretation
R6765	T9555	T9554	punct	–,night
R6766	T9476	T9477	det	the,tasks
R6767	T9477	T9475	pobj	tasks,of
R6768	T9478	T9477	amod	cognitive,tasks
R6769	T9479	T9436	punct	.,feel
R6770	T9481	T9482	prep	In,quantified
R6771	T9556	T9532	prep	than,were
R6772	T9483	T9481	pobj	order,In
R6773	T9484	T9485	aux	to,understand
R6774	T9557	T9556	pobj	any,than
R6775	T9485	T9483	acl	understand,order
R6776	T9558	T9557	prep	of,any
R6777	T9559	T9560	det	the,groups
R6778	T9486	T9487	det	the,nature
R6779	T9487	T9485	dobj	nature,understand
R6780	T9560	T9558	pobj	groups,of
R6781	T9488	T9487	cc	and,nature
R6782	T9489	T9487	conj	extent,nature
R6783	T9490	T9487	prep	of,nature
R6784	T9561	T9560	compound	control,groups
R6785	T9491	T9490	pobj	hyperactivity,of
R6786	T9492	T9491	prep	in,hyperactivity
R6787	T9562	T9532	punct	.,were
R6788	T9493	T9494	det	the,mice
R6789	T9494	T9492	pobj	mice,in
R6790	T9564	T9565	compound	Activity,levels
R6791	T9495	T9496	compound	tTA,APP
R6792	T9496	T9494	compound	APP,mice
R6793	T9497	T9496	punct	/,APP
R6794	T9498	T9482	punct	", ",quantified
R6795	T9565	T9566	nsubj	levels,appeared
R6796	T9499	T9482	nsubj	we,quantified
R6797	T9500	T9501	amod	daily,levels
R6798	T9567	T9568	aux	to,follow
R6799	T9501	T9482	dobj	levels,quantified
R6800	T9502	T9501	compound	activity,levels
R6801	T9503	T9501	prep	in,levels
R6802	T9504	T9505	amod	double,transgenic
R6803	T9568	T9566	xcomp	follow,appeared
R6804	T9505	T9506	amod	transgenic,animals
R6805	T9506	T9503	pobj	animals,in
R6806	T9569	T9570	det	a,cycle
R6807	T9507	T9501	prep	along,levels
R6808	T9508	T9507	prep	with,along
R6809	T9509	T9510	poss	their,siblings
R6810	T9570	T9568	dobj	cycle,follow
R6811	T9510	T9508	pobj	siblings,with
R6812	T9511	T9512	amod	single,transgenic
R6813	T9571	T9572	advmod	relatively,normal
R6814	T9512	T9510	amod	transgenic,siblings
R6815	T9513	T9512	cc	and,transgenic
R6816	T9514	T9512	conj	nontransgenic,transgenic
R6817	T9572	T9570	amod	normal,cycle
R6818	T9515	T9510	acl	using,siblings
R6819	T9516	T9517	nummod	four,beam
R6820	T9517	T9519	compound	beam,frames
R6821	T9518	T9517	punct	-,beam
R6822	T9519	T9515	dobj	frames,using
R6823	T9573	T9570	amod	diurnal,cycle
R6824	T9520	T9519	acl	designed,frames
R6825	T9521	T9522	aux	to,monitor
R6826	T9522	T9520	advcl	monitor,designed
R6827	T9574	T9568	punct	", ",follow
R6828	T9523	T9522	dobj	ambulation,monitor
R6829	T9524	T9522	prep	within,monitor
R6830	T9575	T9568	advcl	decreasing,follow
R6831	T9525	T9526	det	an,cage
R6832	T9526	T9524	pobj	cage,within
R6833	T9527	T9526	amod	enclosed,cage
R6834	T9576	T9575	advmod	substantially,decreasing
R6835	T9528	T9482	punct	.,quantified
R6836	T9577	T9575	prep	during,decreasing
R6837	T9530	T9531	mark	As,shown
R6838	T9531	T9532	advcl	shown,were
R6839	T9578	T9579	det	the,hours
R6840	T9533	T9531	prep	in,shown
R6841	T9534	T9533	pobj	Figure,in
R6842	T9535	T9534	nummod	7,Figure
R6843	T9579	T9577	pobj	hours,during
R6844	T9536	T9532	punct	", ",were
R6845	T9537	T9538	det	the,mice
R6846	T9580	T9579	compound	daylight,hours
R6847	T9538	T9532	nsubj	mice,were
R6848	T9539	T9540	amod	double,transgenic
R6849	T9540	T9538	nmod	transgenic,mice
R6850	T9581	T9566	punct	.,appeared
R6851	T9541	T9542	quantmod	up,10-fold
R6852	T9542	T9544	advmod	10-fold,active
R6853	T9543	T9542	quantmod	to,10-fold
R6855	T9544	T9532	acomp	active,were
R6856	T9545	T9544	advmod	more,active
R6857	T9546	T9532	prep	during,were
R6858	T9547	T9548	det	the,phase
R6859	T9585	T9584	punct	", ",remained
R6860	T9548	T9546	pobj	phase,during
R6861	T9549	T9548	compound	dark,phase
R6862	T9550	T9548	prep	of,phase
R6863	T9586	T9587	advmod	even,during
R6864	T9551	T9552	det	the,cycle
R6865	T9552	T9550	pobj	cycle,of
R6866	T9553	T9554	compound	day,night
R6867	T9587	T9584	prep	during,remained
R6868	T9554	T9552	compound	night,cycle
R6869	T9588	T9589	det	the,phase
R6870	T9589	T9587	pobj	phase,during
R6871	T9590	T9589	compound	light,phase
R6872	T9591	T9584	punct	", ",remained
R6874	T9592	T9593	det	the,mice
R6877	T9593	T9584	nsubj	mice,remained
R6880	T9594	T9595	compound	tTA,APP
R6883	T9595	T9593	compound	APP,mice
R6886	T9596	T9595	punct	/,APP
R6889	T9597	T9598	advmod	many-fold,more
R6892	T9598	T9599	advmod	more,active
R6895	T9599	T9584	acomp	active,remained
R6899	T9600	T9599	prep	than,active
R6903	T9601	T9602	amod	normal,controls
R6906	T9602	T9600	pobj	controls,than
R6910	T9603	T9584	punct	.,remained
R6913	T9605	T9606	det	This,behavior
R6918	T9606	T9607	nsubjpass	behavior,reversed
R6921	T9608	T9607	auxpass	was,reversed
R6922	T9697	T9694	pobj	distinction,with
R6923	T9698	T9697	prep	between,distinction
R6924	T9699	T9700	poss	their,levels
R6925	T9700	T9698	pobj	levels,between
R6926	T9701	T9700	nmod	day,levels
R6927	T9702	T9701	punct	-,day
R6928	T9703	T9701	cc	and,day
R6929	T9609	T9607	advmod	partially,reversed
R6930	T9704	T9701	conj	nighttime,day
R6931	T9705	T9700	compound	activity,levels
R6932	T9706	T9674	punct	.,showed
R6933	T9610	T9609	punct	", ",partially
R6934	T9611	T9609	cc	but,partially
R6935	T9612	T9611	neg	not,but
R6936	T9613	T9609	conj	consistently,partially
R6937	T9614	T9607	punct	", ",reversed
R6938	T9615	T9607	prep	by,reversed
R6939	T9616	T9617	nummod	1,mo
R6940	T9617	T9615	pobj	mo,by
R6941	T9618	T9617	prep	of,mo
R6942	T9619	T9620	compound	transgene,suppression
R6943	T9620	T9618	pobj	suppression,of
R6944	T9621	T9617	acl	beginning,mo
R6945	T9622	T9621	prep	at,beginning
R6946	T9623	T9624	quantmod	4,5
R6947	T9624	T9626	nummod	5,mo
R6948	T9625	T9624	punct	–,5
R6949	T9626	T9622	pobj	mo,at
R6950	T9627	T9626	prep	of,mo
R6951	T9628	T9627	pobj	age,of
R6952	T9629	T9630	punct	(,shown
R6953	T9630	T9607	parataxis	shown,reversed
R6954	T9631	T9630	nsubj	data,shown
R6955	T9632	T9630	neg	not,shown
R6956	T9633	T9630	punct	),shown
R6957	T9634	T9607	punct	.,reversed
R6958	T9636	T9637	prep	In,abolished
R6959	T9638	T9636	pobj	contrast,In
R6960	T9639	T9637	punct	", ",abolished
R6961	T9640	T9637	nsubjpass	hyperactivity,abolished
R6962	T9641	T9637	auxpass	was,abolished
R6963	T9642	T9637	advmod	completely,abolished
R6964	T9643	T9637	prep	by,abolished
R6965	T9644	T9643	pcomp	rearing,by
R6966	T9645	T9646	compound	tTA,APP
R6967	T9646	T9648	compound	APP,mice
R6968	T9647	T9646	punct	/,APP
R6969	T9648	T9644	dobj	mice,rearing
R6970	T9649	T9644	prep	on,rearing
R6971	T9650	T9649	pobj	dox,on
R6972	T9651	T9637	punct	.,abolished
R6973	T9653	T9654	nsubj	Animals,showed
R6974	T9655	T9653	acl	born,Animals
R6975	T9656	T9655	cc	and,born
R6976	T9657	T9655	conj	raised,born
R6977	T9658	T9657	prep	on,raised
R6978	T9659	T9658	pobj	dox,on
R6979	T9660	T9661	compound	activity,levels
R5135	T7755	T7752	prep	via,expressing
R5416	T8054	T8051	appos	mo,mo
R5417	T8055	T8051	amod	treated,mo
R5418	T8056	T8057	punct	(,5
R6074	T8753	T8752	amod	aggregated,Aβ
R6077	T8755	T8754	pobj	either,as
R6078	T8756	T8755	prep	of,either
R6081	T8758	T8756	pobj	groups,of
R6413	T9109	T9110	det	any,changes
R6414	T9110	T9107	attr	changes,were
R6566	T9276	T9264	punct	", ",seen
R6567	T9277	T9264	cc	and,seen
R6854	T9583	T9584	advmod	However,remained
R6873	T9661	T9654	dobj	levels,showed
R6875	T9662	T9661	amod	similar,levels
R6876	T9663	T9662	prep	to,similar
R6878	T9664	T9665	det	the,controls
R6879	T9665	T9663	pobj	controls,to
R6881	T9666	T9665	amod	untreated,controls
R6882	T9667	T9668	punct	(,7C
R6884	T9668	T9654	parataxis	7C,showed
R6885	T9669	T9668	compound	Figure,7C
R6887	T9670	T9668	punct	),7C
R6888	T9671	T9654	punct	.,showed
R6890	T9673	T9674	advmod	Intriguingly,showed
R6891	T9675	T9674	punct	", ",showed
R6893	T9676	T9674	nsubj	all,showed
R6894	T9677	T9676	prep	of,all
R6896	T9678	T9679	det	the,animals
R6897	T9679	T9677	pobj	animals,of
R6898	T9680	T9681	npadvmod	dox,reared
R6900	T9681	T9679	amod	reared,animals
R6901	T9682	T9681	punct	-,reared
R6902	T9683	T9676	punct	", ",all
R6904	T9684	T9685	preconj	both,transgenic
R6905	T9685	T9676	amod	transgenic,all
R6907	T9686	T9685	cc	and,transgenic
R6908	T9687	T9688	amod	wild,type
R6909	T9688	T9685	conj	type,transgenic
R6911	T9689	T9688	punct	-,type
R6912	T9690	T9674	punct	", ",showed
R6914	T9691	T9692	amod	altered,rhythms
R6915	T9692	T9674	dobj	rhythms,showed
R6916	T9693	T9692	amod	circadian,rhythms
R6917	T9694	T9692	prep	with,rhythms
R6919	T9695	T9696	advmod	far,less
R6920	T9696	T9697	amod	less,distinction

2_test

Id	Subject	Object	Predicate	Lexical cue
16279840-8939850-86242765	323-325	8939850	denotes	16
16279840-1319065-86242763	179-181	1319065	denotes	12
16279840-7937760-86242764	182-184	7937760	denotes	13
16279840-9117892-86242766	630-632	9117892	denotes	15
16279840-14645205-86242767	633-635	14645205	denotes	26
16279840-9354339-86242768	636-638	9354339	denotes	27
16279840-8939850-86242769	9810-9812	8939850	denotes	16
16279840-7835246-86242770	9813-9815	7835246	denotes	28
16279840-9354339-86242771	10333-10335	9354339	denotes	27
16279840-10493755-86242772	10748-10750	10493755	denotes	29
16279840-12218651-86242773	18423-18425	12218651	denotes	22
16279840-11160418-86242774	19365-19367	11160418	denotes	23
16279840-12151510-86242775	24495-24497	12151510	denotes	30
16279840-15755686-86242776	24900-24902	15755686	denotes	31
T47654	179-181	1319065	denotes	12
T45338	182-184	7937760	denotes	13
T42231	323-325	8939850	denotes	16
T248	630-632	9117892	denotes	15
T42200	633-635	14645205	denotes	26
T15428	636-638	9354339	denotes	27
T30577	9810-9812	8939850	denotes	16
T87295	9813-9815	7835246	denotes	28
T15927	10333-10335	9354339	denotes	27
T56717	10748-10750	10493755	denotes	29
T30455	18423-18425	12218651	denotes	22
T72545	19365-19367	11160418	denotes	23
T85669	24495-24497	12151510	denotes	30
T73868	24900-24902	15755686	denotes	31

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T6117	28-37	SO_EXT:0000902	denotes	transgene
T6118	48-55	GO:0010467	denotes	express
T6119	65-71	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6120	92-94	NCBITaxon:10088	denotes	mo
T6121	145-157	CHEBI:27902	denotes	tetracycline
T6122	169-177	SO_EXT:0000167	denotes	promoter
T6123	187-197	SO_EXT:transgenic_entity	denotes	Transgenic
T6124	198-201	PR_EXT:000004168	denotes	APP
T6125	202-212	GO:0010467	denotes	expression
T6126	254-258	NCBITaxon:10088	denotes	mice
T6127	262-269	NCBITaxon:33208	denotes	animals
T6128	290-297	GO:0065007	denotes	control
T6129	305-312	PR_EXT:000003199	denotes	CaMKIIα
T6130	313-321	SO_EXT:0000167	denotes	promoter
T6131	408-412	NCBITaxon:10088	denotes	mice
T6132	447-456	SO_EXT:0000902	denotes	transgene
T6133	480-490	GO:0010467	denotes	expressing
T6134	545-548	PR_EXT:000004168	denotes	APP
T6135	549-559	SO_EXT:transgenic_entity	denotes	transgenic
T6136	583-590	CHEBI:60425	denotes	amyloid
T6137	696-706	SO_EXT:transgenic_entity	denotes	transgenic
T6138	707-710	PR_EXT:000004168	denotes	APP
T6139	711-718	CHEBI_PR_EXT:protein	denotes	protein
T6140	825-828	PR_EXT:000004168	denotes	APP
T6141	842-852	SO_EXT:transgenic_entity	denotes	transgenic
T6142	865-868	PR_EXT:000004168	denotes	APP
T6143	869-873	NCBITaxon:10088	denotes	mice
T6144	883-891	GO:0042571	denotes	antibody
T6145	924-927	PR_EXT:000004168	denotes	APP
T6146	933-940	CHEBI_PR_EXT:amyloid_beta	denotes	amyloid
T6147	933-965	PR_EXT:000004137	denotes	amyloid precursor-like protein 2
T6148	956-963	CHEBI_PR_EXT:protein	denotes	protein
T6149	975-985	SO_EXT:transgenic_entity	denotes	transgenic
T6150	986-993	CHEBI_PR_EXT:protein	denotes	protein
T6151	1006-1014	GO:0042571	denotes	antibody
T6152	2723-2726	PR_EXT:000004168	denotes	APP
T6153	2727-2731	NCBITaxon:10088	denotes	mice
T6154	2774-2783	SO_EXT:0000902	denotes	transgene
T6155	2794-2797	CHEBI:50845	denotes	dox
T6156	2942-2951	SO_EXT:0000902	denotes	transgene
T6157	3013-3016	CHEBI:50845	denotes	dox
T6158	3022-3025	CHEBI:50845	denotes	dox
T6159	3093-3097	NCBITaxon:10088	denotes	mice
T6160	3102-3106	GO:0007567	denotes	born
T6161	3121-3124	CHEBI:50845	denotes	dox
T6162	3158-3161	CHEBI:50845	denotes	dox
T6163	3208-3211	CHEBI:50845	denotes	dox
T6164	3250-3254	CHEBI_EXT:33290	denotes	chow
T6165	3298-3305	NCBITaxon:33208	denotes	Animals
T6166	3306-3310	GO:0007567	denotes	born
T6167	3325-3328	CHEBI:50845	denotes	dox
T6168	3341-3351	SO_EXT:transgenic_entity	denotes	transgenic
T6169	3352-3355	PR_EXT:000004168	denotes	APP
T6170	3400-3403	CHEBI:50845	denotes	dox
T6171	3415-3425	SO_EXT:transgenic_entity	denotes	transgenic
T6172	3426-3429	PR_EXT:000004168	denotes	APP
T6173	3430-3440	GO:0010467	denotes	expression
T6174	3486-3489	CHEBI:50845	denotes	dox
T6175	3511-3521	GO:0010467	denotes	expression
T6176	3551-3555	NCBITaxon:10088	denotes	mice
T6177	3587-3597	SO_EXT:transgenic_entity	denotes	transgenic
T6178	3598-3605	CHEBI_PR_EXT:protein	denotes	protein
T6179	3633-3636	CHEBI:50845	denotes	dox
T6180	3705-3718	GO_EXT:transcription	denotes	transcription
T6181	3770-3773	PR_EXT:000004168	denotes	APP
T6182	3791-3801	SO_EXT:transgenic_entity	denotes	transgenic
T6183	3815-3820	PR_EXT:APLP	denotes	APLPs
T6184	3859-3866	NCBITaxon:33208	denotes	animals
T6185	3919-3929	SO_EXT:transgenic_entity	denotes	transgenic
T6186	3930-3934	NCBITaxon:10088	denotes	mice
T6187	4071-4073	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6188	4071-4084	GO:0034205	denotes	Aβ production
T6189	4120-4123	CHEBI:50845	denotes	dox
T6190	4185-4189	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T6191	4194-4198	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6192	4218-4227	UBERON:0001890	denotes	forebrain
T6193	4259-4266	NCBITaxon:33208	denotes	animals
T6194	4288-4292	NCBITaxon:10088	denotes	mice
T6195	4308-4315	CHEBI:60425	denotes	amyloid
T6196	4373-4380	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6197	4398-4403	UBERON:0000955	denotes	brain
T6198	4414-4423	SO_EXT:0000902	denotes	transgene
T6199	4551-4558	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6200	4551-4568	GO:0043043	denotes	peptide synthesis
T6201	4631-4634	CHEBI:8984	denotes	SDS
T6202	4644-4646	CHEBI:30751	denotes	FA
T6203	4667-4675	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptides
T6204	4717-4722	NCBITaxon:9606	denotes	human
T6205	4723-4732	SO_EXT:0000902	denotes	transgene
T6206	4741-4745	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T6207	4750-4754	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6208	4768-4772	NCBITaxon:10088	denotes	mice
T6209	4797-4804	NCBITaxon:33208	denotes	animals
T6210	4826-4830	GO:0007567	denotes	born
T6211	4845-4848	CHEBI:50845	denotes	dox
T6212	4909-4912	CHEBI:50845	denotes	dox
T6213	4913-4917	CHEBI_EXT:33290	denotes	chow
T6214	5150-5159	SO_EXT:0000902	denotes	transgene
T6215	5168-5170	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6216	5208-5215	NCBITaxon:33208	denotes	animals
T6217	5216-5220	GO:0007567	denotes	born
T6218	5235-5238	CHEBI:50845	denotes	dox
T6219	5288-5298	CHEBI_EXT:33282	denotes	antibiotic
T6220	5355-5359	NCBITaxon:10088	denotes	mice
T6221	5373-5377	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6222	5413-5416	CHEBI:50845	denotes	dox
T6223	5487-5490	CHEBI:8984	denotes	SDS
T6224	5499-5503	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6225	5560-5563	CHEBI:50845	denotes	dox
T6226	5596-5598	CHEBI:30751	denotes	FA
T6227	5625-5628	CHEBI:50845	denotes	dox
T6228	5647-5654	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6229	5674-5681	NCBITaxon:33208	denotes	animals
T6230	5829-5836	NCBITaxon:33208	denotes	animals
T6231	5847-5851	GO:0007567	denotes	born
T6232	5866-5869	CHEBI:50845	denotes	dox
T6233	5923-5930	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6234	5948-5952	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6235	5977-5981	NCBITaxon:10088	denotes	mice
T6236	6004-6006	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6237	6030-6034	NCBITaxon:10088	denotes	mice
T6238	6061-6070	SO_EXT:0000902	denotes	transgene
T6239	6079-6086	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6240	6106-6108	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6241	6127-6130	PR_EXT:000004168	denotes	APP
T6242	6131-6135	NCBITaxon:10088	denotes	mice
T6243	6176-6183	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6244	6196-6206	SO_EXT:transgenic_entity	denotes	transgenic
T6245	6207-6214	NCBITaxon:33208	denotes	animals
T6246	6337-6340	CHEBI:50845	denotes	dox
T6247	6365-6375	SO_EXT:transgenic_entity	denotes	transgenic
T6248	6428-6430	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6249	9311-9320	SO_EXT:sequence_alteration_entity_or_process	denotes	mutations
T6250	9343-9347	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6251	9417-9424	NCBITaxon:33208	denotes	animals
T6252	9466-9476	SO_EXT:transgenic_entity	denotes	transgenic
T6253	9478-9485	PR_EXT:000003199	denotes	CaMKIIα
T6254	9508-9512	NCBITaxon:10088	denotes	mice
T6255	9534-9541	CHEBI:60425	denotes	amyloid
T6256	9575-9582	CHEBI:60425	denotes	Amyloid
T6257	9604-9608	NCBITaxon:10088	denotes	mice
T6258	9679-9688	UBERON:0001890	denotes	forebrain
T6259	9704-9710	UBERON:0001851	denotes	cortex
T6260	9712-9723	UBERON_EXT:hippocampus_proper_or_hippocampal_formation	denotes	hippocampus
T6261	9725-9734	GO:0007608	denotes	olfactory
T6262	9725-9739	UBERON:0002264	denotes	olfactory bulb
T6263	9745-9753	UBERON:0002435	denotes	striatum
T6264	9765-9772	PR_EXT:000003199	denotes	CaMKIIα
T6265	9773-9781	SO_EXT:0000167	denotes	promoter
T6266	9846-9853	CHEBI:60425	denotes	amyloid
T6267	9904-9910	UBERON:0001851	denotes	cortex
T6268	9915-9926	UBERON_EXT:hippocampus_proper_or_hippocampal_formation	denotes	hippocampus
T6269	9968-9978	UBERON:0002037	denotes	cerebellum
T6270	9982-9992	UBERON:0002298	denotes	brain stem
T6271	10028-10035	PR_EXT:000003199	denotes	CaMKIIα
T6272	10036-10046	GO:0065007	denotes	controlled
T6273	10047-10056	SO_EXT:0000902	denotes	transgene
T6274	10057-10067	GO:0010467	denotes	expression
T6275	10108-10113	NCBITaxon:9606	denotes	human
T6276	10164-10167	PR_EXT:000004168	denotes	APP
T6277	10168-10172	NCBITaxon:10088	denotes	mice
T6278	10279-10282	PR_EXT:000004168	denotes	APP
T6279	10283-10293	SO_EXT:transgenic_entity	denotes	transgenic
T6280	10294-10298	NCBITaxon:10088	denotes	mice
T6281	10323-10331	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T6282	10384-10387	PR_EXT:000004168	denotes	APP
T6283	10388-10392	NCBITaxon:10088	denotes	mice
T6284	10465-10472	CHEBI:60425	denotes	amyloid
T6285	10502-10510	UBERON:0001897	denotes	thalamus
T6286	10544-10548	NCBITaxon:10088	denotes	mice
T6287	10549-10559	GO:0010467	denotes	expressing
T6288	10560-10566	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6289	10567-10570	PR_EXT:000004168	denotes	APP
T6290	10579-10584	PR_EXT:000001843	denotes	Thy-1
T6291	10585-10593	SO_EXT:0000167	denotes	promoter
T6292	10611-10618	CHEBI:60425	denotes	amyloid
T6293	10667-10673	GO:0030424	denotes	axonal
T6294	10667-10683	GO:0008088	denotes	axonal transport
T6295	10687-10690	PR_EXT:000004168	denotes	APP
T6296	10691-10693	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6297	10701-10713	_FRAGMENT	denotes	terminals of
T6298	10723-10730	GO_UBERON_EXT:nerve_ending_or_neuron_projection_terminus	denotes	neurons
T6299	10714-10722	UBERON:0001851	denotes	cortical
T6300	10714-10730	CL:0010012	denotes	cortical neurons
T6301	10738-10746	UBERON:0001897	denotes	thalamus
T6302	10783-10793	SO_EXT:transgenic_entity	denotes	transgenic
T6303	10794-10798	NCBITaxon:10088	denotes	mice
T6304	10800-10810	GO:0010467	denotes	expressing
T6305	10828-10831	PR_EXT:000004168	denotes	APP
T6306	10832-10842	SO_EXT:0000902	denotes	transgenes
T6307	10854-10861	CHEBI:60425	denotes	amyloid
T6308	10878-10888	SO_EXT:transgenic_entity	denotes	transgenic
T6309	10889-10893	NCBITaxon:10088	denotes	mice
T6310	10965-10972	CHEBI:60425	denotes	amyloid
T6311	11021-11031	SO_EXT:transgenic_entity	denotes	transgenic
T6312	11032-11039	NCBITaxon:33208	denotes	animals
T6313	11040-11044	GO:0007567	denotes	born
T6314	11059-11062	CHEBI:50845	denotes	dox
T6315	11064-11071	NCBITaxon:33208	denotes	Animals
T6316	11089-11099	GO:0010467	denotes	expressing
T6317	11130-11133	CHEBI:50845	denotes	dox
T6318	11160-11167	CHEBI:60425	denotes	amyloid
T6319	11232-11241	SO_EXT:0000902	denotes	transgene
T6320	11242-11252	GO:0010467	denotes	expression
T6321	11272-11275	CHEBI:50845	denotes	dox
T6322	11304-11306	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6323	11307-11314	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6324	11325-11332	CHEBI:60425	denotes	amyloid
T6325	11406-11416	CHEBI_EXT:35222	denotes	inhibitors
T6326	11420-11422	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6327	11420-11433	GO:0034205	denotes	Aβ production
T6328	11466-11476	SO_EXT:transgenic_entity	denotes	transgenic
T6329	11477-11481	NCBITaxon:10088	denotes	mice
T6330	11483-11490	PR_EXT:000003199	denotes	CaMKIIα
T6331	11497-11500	PR_EXT:000004168	denotes	APP
T6332	11521-11525	CHEBI_EXT:33290	denotes	food
T6333	11558-11565	CHEBI:60425	denotes	amyloid
T6334	11609-11614	UBERON:0000955	denotes	brain
T6335	11637-11644	NCBITaxon:33208	denotes	animals
T6336	11671-11675	CHEBI_EXT:33290	denotes	chow
T6337	11679-11683	CHEBI_EXT:33290	denotes	food
T6338	11695-11698	CHEBI:50845	denotes	dox
T6339	11728-11738	GO_EXT:killing	denotes	sacrificed
T6340	11783-11790	NCBITaxon:33208	denotes	animals
T6341	11813-11817	CHEBI_EXT:33290	denotes	chow
T6342	12003-12013	SO_EXT:transgenic_entity	denotes	transgenic
T6343	12039-12042	CHEBI:50845	denotes	dox
T6344	12051-12058	NCBITaxon:33208	denotes	animals
T6345	12118-12127	SO_EXT:0000902	denotes	transgene
T6346	12171-12175	NCBITaxon:10088	denotes	mice
T6347	12232-12239	NCBITaxon:33208	denotes	animals
T6348	12282-12289	NCBITaxon:33208	denotes	animals
T6349	12295-12298	CHEBI:50845	denotes	dox
T6350	12359-12362	PR_EXT:000004168	denotes	APP
T6351	12363-12373	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminal
T6352	12502-12511	SO_EXT:sequence_alteration_process	denotes	processed
T6353	12512-12522	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminal
T6354	14313-14319	UBERON:0000479	denotes	Tissue
T6355	14339-14345	NCBITaxon:33208	denotes	animal
T6356	14392-14399	CHEBI:60425	denotes	amyloid
T6357	14420-14426	CHEBI_EXT:silver	denotes	silver
T6358	14445-14451	CHEBI_EXT:silver	denotes	silver
T6359	14471-14473	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6360	14554-14561	CHEBI:60425	denotes	amyloid
T6361	14626-14633	CHEBI:60425	denotes	amyloid
T6362	14669-14676	CHEBI:60425	denotes	amyloid
T6363	14690-14694	NCBITaxon:10088	denotes	mice
T6364	14959-14966	NCBITaxon:33208	denotes	animals
T6365	14981-14990	SO_EXT:0000902	denotes	transgene
T6366	15111-15118	CHEBI:60425	denotes	amyloid
T6367	15208-15214	CHEBI_EXT:silver	denotes	silver
T6368	15255-15262	CHEBI:60425	denotes	amyloid
T6369	15370-15373	CHEBI:50845	denotes	dox
T6370	15397-15407	SO_EXT:transgenic_entity	denotes	transgenic
T6371	15408-15411	PR_EXT:000004168	denotes	APP
T6372	17234-17238	NCBITaxon:10088	denotes	mice
T6373	17256-17259	CHEBI:50845	denotes	dox
T6374	17311-17314	PR_EXT:000004168	denotes	APP
T6375	17315-17319	NCBITaxon:10088	denotes	mice
T6376	17321-17328	PR_EXT:000003199	denotes	CaMKIIα
T6377	17385-17388	CHEBI:50845	denotes	dox
T6378	17429-17433	NCBITaxon:10088	denotes	mice
T6379	17463-17472	SO_EXT:0000902	denotes	transgene
T6380	17580-17583	PR_EXT:000004168	denotes	APP
T6381	17599-17608	SO_EXT:0000902	denotes	transgene
T6382	17636-17643	NCBITaxon:33208	denotes	animals
T6383	17676-17680	NCBITaxon:10088	denotes	mice
T6384	17698-17705	CHEBI:60425	denotes	amyloid
T6385	17753-17757	NCBITaxon:10088	denotes	mice
T6386	17800-17809	SO_EXT:0000902	denotes	transgene
T6387	17810-17820	GO:0010467	denotes	expression
T6388	17923-17929	CHEBI_EXT:silver	denotes	silver
T6389	18001-18004	CHEBI:50845	denotes	dox
T6390	18013-18020	NCBITaxon:33208	denotes	animals
T6391	18025-18038	CHEBI_EXT:biochemical	denotes	biochemically
T6392	18073-18075	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6393	18083-18089	UBERON:0000955	denotes	brains
T6394	18097-18101	NCBITaxon:10088	denotes	mice
T6395	18119-18128	SO_EXT:0000902	denotes	transgene
T6396	18171-18179	UBERON:0001851	denotes	cortical
T6397	18180-18186	UBERON:0000479	denotes	tissue
T6398	18197-18203	NCBITaxon:33208	denotes	animal
T6399	18240-18247	CHEBI_PR_EXT:protein	denotes	protein
T6400	18263-18269	GO_EXT:biological_movement_or_translocation_process	denotes	passed
T6401	18448-18454	GO:0007601	denotes	visual
T6402	18507-18510	PR_EXT:000004168	denotes	APP
T6403	18511-18515	NCBITaxon:10088	denotes	mice
T6405	18581-18583	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6406	18682-18686	NCBITaxon:10088	denotes	mice
T6407	18723-18725	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6408	18795-18798	PR_EXT:000004168	denotes	APP
T6409	18799-18803	NCBITaxon:10088	denotes	mice
T6410	18856-18858	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6411	18881-18888	NCBITaxon:33208	denotes	animals
T6412	18942-18951	SO_EXT:0000902	denotes	transgene
T6413	19022-19024	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6414	19032-19038	UBERON:0000955	denotes	brains
T6415	19116-19123	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6416	19150-19160	GO:0010467	denotes	expression
T6417	19177-19185	UBERON:0001851	denotes	Cortical
T6418	19238-19245	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6419	19257-19260	CHEBI:8984	denotes	SDS
T6420	19267-19269	CHEBI:30751	denotes	FA
T6421	19294-19303	SO_EXT:0000902	denotes	transgene
T6422	19312-19316	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T6423	19321-19325	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6424	19343-19348	NCBITaxon:9606	denotes	human
T6425	19377-19384	NCBITaxon:33208	denotes	animals
T6426	19395-19402	CHEBI:60425	denotes	amyloid
T6427	19448-19450	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6428	19481-19484	CHEBI:8984	denotes	SDS
T6429	19489-19491	CHEBI:30751	denotes	FA
T6430	19620-19623	CHEBI:8984	denotes	SDS
T6431	19628-19630	CHEBI:30751	denotes	FA
T6432	19639-19641	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6433	19752-19758	UBERON:0000955	denotes	brains
T6434	19855-19859	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T6435	19882-19886	NCBITaxon:10088	denotes	mice
T6436	19910-19914	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6437	20014-20018	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T6438	20023-20027	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6439	20227-20234	NCBITaxon:33208	denotes	animals
T6440	20279-20281	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6441	20291-20298	NCBITaxon:33208	denotes	animals
T6442	22471-22479	GO:0043005	denotes	neuritic
T6443	22484-22489	CL:0000125	denotes	glial
T6444	22605-22614	SO_EXT:0000902	denotes	transgene
T6445	22640-22646	CHEBI_EXT:silver	denotes	silver
T6446	22657-22666	PR_EXT:ubiquitin	denotes	ubiquitin
T6447	22689-22697	GO:0043005	denotes	neuritic
T6448	22755-22775	CL:0002627	denotes	activated astrocytes
T6449	22794-22798	PR_EXT:000007939	denotes	GFAP
T6450	22830-22837	NCBITaxon:33208	denotes	animals
T6451	22850-22858	GO:0043005	denotes	Neuritic
T6452	22863-22868	CL:0000125	denotes	glial
T6453	22919-22923	NCBITaxon:10088	denotes	mice
T6454	22938-22947	SO_EXT:0000902	denotes	transgene
T6455	22974-22980	GO_EXT:biological_growth_entity_or_process	denotes	growth
T6456	23026-23030	NCBITaxon:10088	denotes	mice
T6457	23763-23765	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6458	23777-23786	GO:0050890	denotes	cognitive
T6459	23808-23812	NCBITaxon:10088	denotes	mice
T6460	23839-23848	GO:0050890	denotes	cognition
T6461	23922-23931	GO:0050890	denotes	cognitive
T6462	23932-23940	GO_PATO_EXT:biological_behavior	denotes	behavior
T6463	24002-24012	SO_EXT:transgenic_entity	denotes	transgenic
T6464	24013-24017	NCBITaxon:10088	denotes	mice
T6465	24027-24030	PR_EXT:000004168	denotes	APP
T6466	24031-24038	NCBITaxon:33208	denotes	animals
T6467	24125-24133	GO:0036268	denotes	swimming
T6468	24161-24165	NCBITaxon:10088	denotes	mice
T6469	24192-24197	CHEBI:15377	denotes	water
T6470	24218-24223	CHEBI:15377	denotes	water
T6471	24241-24245	GO:0036268	denotes	swim
T6472	24372-24379	NCBITaxon:33208	denotes	animals
T6473	24450-24454	NCBITaxon:10088	denotes	mice
T6474	24592-24599	NCBITaxon:33208	denotes	animals
T6475	24658-24662	NCBITaxon:10088	denotes	mice
T6476	24714-24721	GO:0010467	denotes	express
T6477	24738-24748	SO_EXT:transgenic_entity	denotes	transgenic
T6478	24749-24756	CHEBI_PR_EXT:protein	denotes	protein
T6479	24864-24871	CHEBI:60425	denotes	amyloid
T6480	24881-24890	GO:0050890	denotes	cognitive
T6481	24990-24993	PR_EXT:000004168	denotes	APP
T6482	24994-24998	NCBITaxon:10088	denotes	mice
T6483	25048-25057	GO:0050890	denotes	cognitive
T6484	25074-25081	NCBITaxon:33208	denotes	animals
T6485	25131-25140	GO:0050890	denotes	cognitive
T6486	25232-25235	PR_EXT:000004168	denotes	APP
T6487	25236-25240	NCBITaxon:10088	denotes	mice
T6488	25288-25298	SO_EXT:transgenic_entity	denotes	transgenic
T6489	25299-25306	NCBITaxon:33208	denotes	animals
T6490	25331-25341	SO_EXT:transgenic_entity	denotes	transgenic
T6404	18529-18532	CHEBI:50845	denotes	dox
T6491	25349-25359	SO_EXT:transgenic_entity	denotes	transgenic
T6492	25412-25422	GO:0040011	denotes	ambulation
T6493	25481-25491	SO_EXT:transgenic_entity	denotes	transgenic
T6494	25492-25496	NCBITaxon:10088	denotes	mice
T6495	25660-25673	GO:0007623	denotes	diurnal cycle
T6496	25773-25776	PR_EXT:000004168	denotes	APP
T6497	25777-25781	NCBITaxon:10088	denotes	mice
T6498	25840-25848	GO_PATO_EXT:biological_behavior	denotes	behavior
T6499	25906-25915	SO_EXT:0000902	denotes	transgene
T6500	26040-26043	PR_EXT:000004168	denotes	APP
T6501	26044-26048	NCBITaxon:10088	denotes	mice
T6502	26052-26055	CHEBI:50845	denotes	dox
T6503	26057-26064	NCBITaxon:33208	denotes	Animals
T6504	26065-26069	GO:0007567	denotes	born
T6505	26084-26087	CHEBI:50845	denotes	dox
T6506	26183-26186	CHEBI:50845	denotes	dox
T6507	26194-26201	NCBITaxon:33208	denotes	animals
T6508	26208-26218	SO_EXT:transgenic_entity	denotes	transgenic
T6509	26223-26232	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T6510	26249-26266	GO:0007623	denotes	circadian rhythms
R3966	T6298	T6297	_lexicallyChainedTo	neurons,terminals of

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T5826	48-55	GO:0010467	denotes	express
T5827	92-94	NCBITaxon:10088	denotes	mo
T5828	145-157	CHEBI:27902	denotes	tetracycline
T5829	169-177	SO:0000167	denotes	promoter
T5830	198-201	PR:000004168	denotes	APP
T5831	202-212	GO:0010467	denotes	expression
T5832	254-258	NCBITaxon:10088	denotes	mice
T5833	262-269	NCBITaxon:33208	denotes	animals
T5834	290-297	GO:0065007	denotes	control
T5835	305-312	PR:000003199	denotes	CaMKIIα
T5836	313-321	SO:0000167	denotes	promoter
T5837	408-412	NCBITaxon:10088	denotes	mice
T5838	447-456	SO:0000902	denotes	transgene
T5839	480-490	GO:0010467	denotes	expressing
T5840	545-548	PR:000004168	denotes	APP
T5841	583-590	CHEBI:60425	denotes	amyloid
T5842	707-710	PR:000004168	denotes	APP
T5843	825-828	PR:000004168	denotes	APP
T5844	865-868	PR:000004168	denotes	APP
T5845	869-873	NCBITaxon:10088	denotes	mice
T5846	883-891	GO:0042571	denotes	antibody
T5847	924-927	PR:000004168	denotes	APP
T5848	933-965	PR:000004137	denotes	amyloid precursor-like protein 2
T5849	1006-1014	GO:0042571	denotes	antibody
T5850	2723-2726	PR:000004168	denotes	APP
T5851	2727-2731	NCBITaxon:10088	denotes	mice
T5852	2774-2783	SO:0000902	denotes	transgene
T5853	2794-2797	CHEBI:50845	denotes	dox
T5854	2942-2951	SO:0000902	denotes	transgene
T5855	3013-3016	CHEBI:50845	denotes	dox
T5856	3022-3025	CHEBI:50845	denotes	dox
T5857	3093-3097	NCBITaxon:10088	denotes	mice
T5858	3102-3106	GO:0007567	denotes	born
T5859	3121-3124	CHEBI:50845	denotes	dox
T5860	3158-3161	CHEBI:50845	denotes	dox
T5861	3208-3211	CHEBI:50845	denotes	dox
T5862	3250-3254	CHEBI:33290	denotes	chow
T5863	3298-3305	NCBITaxon:33208	denotes	Animals
T5864	3306-3310	GO:0007567	denotes	born
T5865	3325-3328	CHEBI:50845	denotes	dox
T5866	3352-3355	PR:000004168	denotes	APP
T5867	3400-3403	CHEBI:50845	denotes	dox
T5868	3426-3429	PR:000004168	denotes	APP
T5869	3430-3440	GO:0010467	denotes	expression
T5870	3486-3489	CHEBI:50845	denotes	dox
T5871	3511-3521	GO:0010467	denotes	expression
T5872	3551-3555	NCBITaxon:10088	denotes	mice
T5873	3633-3636	CHEBI:50845	denotes	dox
T5874	3770-3773	PR:000004168	denotes	APP
T5875	3859-3866	NCBITaxon:33208	denotes	animals
T5876	3930-3934	NCBITaxon:10088	denotes	mice
T5877	4071-4084	GO:0034205	denotes	Aβ production
T5878	4120-4123	CHEBI:50845	denotes	dox
T5879	4218-4227	UBERON:0001890	denotes	forebrain
T5880	4259-4266	NCBITaxon:33208	denotes	animals
T5881	4288-4292	NCBITaxon:10088	denotes	mice
T5882	4308-4315	CHEBI:60425	denotes	amyloid
T5883	4398-4403	UBERON:0000955	denotes	brain
T5884	4414-4423	SO:0000902	denotes	transgene
T5885	4551-4568	GO:0043043	denotes	peptide synthesis
T5886	4631-4634	CHEBI:8984	denotes	SDS
T5887	4644-4646	CHEBI:30751	denotes	FA
T5888	4717-4722	NCBITaxon:9606	denotes	human
T5889	4723-4732	SO:0000902	denotes	transgene
T5890	4768-4772	NCBITaxon:10088	denotes	mice
T5891	4797-4804	NCBITaxon:33208	denotes	animals
T5892	4826-4830	GO:0007567	denotes	born
T5893	4845-4848	CHEBI:50845	denotes	dox
T5894	4909-4912	CHEBI:50845	denotes	dox
T5895	4913-4917	CHEBI:33290	denotes	chow
T5896	5150-5159	SO:0000902	denotes	transgene
T5897	5208-5215	NCBITaxon:33208	denotes	animals
T5898	5216-5220	GO:0007567	denotes	born
T5899	5235-5238	CHEBI:50845	denotes	dox
T5900	5288-5298	CHEBI:33282	denotes	antibiotic
T5901	5355-5359	NCBITaxon:10088	denotes	mice
T5902	5413-5416	CHEBI:50845	denotes	dox
T5903	5487-5490	CHEBI:8984	denotes	SDS
T5904	5560-5563	CHEBI:50845	denotes	dox
T5905	5596-5598	CHEBI:30751	denotes	FA
T5906	5625-5628	CHEBI:50845	denotes	dox
T5907	5674-5681	NCBITaxon:33208	denotes	animals
T5908	5829-5836	NCBITaxon:33208	denotes	animals
T5909	5847-5851	GO:0007567	denotes	born
T5910	5866-5869	CHEBI:50845	denotes	dox
T5911	5977-5981	NCBITaxon:10088	denotes	mice
T5912	6030-6034	NCBITaxon:10088	denotes	mice
T5913	6061-6070	SO:0000902	denotes	transgene
T5914	6127-6130	PR:000004168	denotes	APP
T5915	6131-6135	NCBITaxon:10088	denotes	mice
T5916	6207-6214	NCBITaxon:33208	denotes	animals
T5917	6337-6340	CHEBI:50845	denotes	dox
T5918	9417-9424	NCBITaxon:33208	denotes	animals
T5919	9478-9485	PR:000003199	denotes	CaMKIIα
T5920	9508-9512	NCBITaxon:10088	denotes	mice
T5921	9534-9541	CHEBI:60425	denotes	amyloid
T5922	9575-9582	CHEBI:60425	denotes	Amyloid
T5923	9604-9608	NCBITaxon:10088	denotes	mice
T5924	9679-9688	UBERON:0001890	denotes	forebrain
T5925	9704-9710	UBERON:0001851	denotes	cortex
T5926	9725-9734	GO:0007608	denotes	olfactory
T5927	9725-9739	UBERON:0002264	denotes	olfactory bulb
T5928	9745-9753	UBERON:0002435	denotes	striatum
T5929	9765-9772	PR:000003199	denotes	CaMKIIα
T5930	9773-9781	SO:0000167	denotes	promoter
T5931	9846-9853	CHEBI:60425	denotes	amyloid
T5932	9904-9910	UBERON:0001851	denotes	cortex
T5933	9968-9978	UBERON:0002037	denotes	cerebellum
T5934	9982-9992	UBERON:0002298	denotes	brain stem
T5935	10028-10035	PR:000003199	denotes	CaMKIIα
T5936	10036-10046	GO:0065007	denotes	controlled
T5937	10047-10056	SO:0000902	denotes	transgene
T5938	10057-10067	GO:0010467	denotes	expression
T5939	10108-10113	NCBITaxon:9606	denotes	human
T5940	10164-10167	PR:000004168	denotes	APP
T5941	10168-10172	NCBITaxon:10088	denotes	mice
T5942	10279-10282	PR:000004168	denotes	APP
T5943	10294-10298	NCBITaxon:10088	denotes	mice
T5944	10384-10387	PR:000004168	denotes	APP
T5945	10388-10392	NCBITaxon:10088	denotes	mice
T5946	10465-10472	CHEBI:60425	denotes	amyloid
T5947	10502-10510	UBERON:0001897	denotes	thalamus
T5948	10544-10548	NCBITaxon:10088	denotes	mice
T5949	10549-10559	GO:0010467	denotes	expressing
T5950	10567-10570	PR:000004168	denotes	APP
T5951	10579-10584	PR:000001843	denotes	Thy-1
T5952	10585-10593	SO:0000167	denotes	promoter
T5953	10611-10618	CHEBI:60425	denotes	amyloid
T5954	10667-10673	GO:0030424	denotes	axonal
T5955	10667-10683	GO:0008088	denotes	axonal transport
T5956	10687-10690	PR:000004168	denotes	APP
T5957	10714-10722	UBERON:0001851	denotes	cortical
T5958	10714-10730	CL:0010012	denotes	cortical neurons
T5959	10738-10746	UBERON:0001897	denotes	thalamus
T5960	10794-10798	NCBITaxon:10088	denotes	mice
T5961	10800-10810	GO:0010467	denotes	expressing
T5962	10828-10831	PR:000004168	denotes	APP
T5963	10832-10842	SO:0000902	denotes	transgenes
T5964	10854-10861	CHEBI:60425	denotes	amyloid
T5965	10889-10893	NCBITaxon:10088	denotes	mice
T5966	10965-10972	CHEBI:60425	denotes	amyloid
T5967	11032-11039	NCBITaxon:33208	denotes	animals
T5968	11040-11044	GO:0007567	denotes	born
T5969	11059-11062	CHEBI:50845	denotes	dox
T5970	11064-11071	NCBITaxon:33208	denotes	Animals
T5971	11089-11099	GO:0010467	denotes	expressing
T5972	11130-11133	CHEBI:50845	denotes	dox
T5973	11160-11167	CHEBI:60425	denotes	amyloid
T5974	11232-11241	SO:0000902	denotes	transgene
T5975	11242-11252	GO:0010467	denotes	expression
T5976	11272-11275	CHEBI:50845	denotes	dox
T5977	11325-11332	CHEBI:60425	denotes	amyloid
T5978	11406-11416	CHEBI:35222	denotes	inhibitors
T5979	11420-11433	GO:0034205	denotes	Aβ production
T5980	11477-11481	NCBITaxon:10088	denotes	mice
T5981	11483-11490	PR:000003199	denotes	CaMKIIα
T5982	11497-11500	PR:000004168	denotes	APP
T5983	11521-11525	CHEBI:33290	denotes	food
T5984	11558-11565	CHEBI:60425	denotes	amyloid
T5985	11609-11614	UBERON:0000955	denotes	brain
T5986	11637-11644	NCBITaxon:33208	denotes	animals
T5987	11671-11675	CHEBI:33290	denotes	chow
T5988	11679-11683	CHEBI:33290	denotes	food
T5989	11695-11698	CHEBI:50845	denotes	dox
T5990	11783-11790	NCBITaxon:33208	denotes	animals
T5991	11813-11817	CHEBI:33290	denotes	chow
T5992	12039-12042	CHEBI:50845	denotes	dox
T5993	12051-12058	NCBITaxon:33208	denotes	animals
T5994	12118-12127	SO:0000902	denotes	transgene
T5995	12171-12175	NCBITaxon:10088	denotes	mice
T5996	12232-12239	NCBITaxon:33208	denotes	animals
T5997	12282-12289	NCBITaxon:33208	denotes	animals
T5998	12295-12298	CHEBI:50845	denotes	dox
T5999	12359-12362	PR:000004168	denotes	APP
T6000	14313-14319	UBERON:0000479	denotes	Tissue
T6001	14339-14345	NCBITaxon:33208	denotes	animal
T6002	14392-14399	CHEBI:60425	denotes	amyloid
T6003	14554-14561	CHEBI:60425	denotes	amyloid
T6004	14626-14633	CHEBI:60425	denotes	amyloid
T6005	14669-14676	CHEBI:60425	denotes	amyloid
T6006	14690-14694	NCBITaxon:10088	denotes	mice
T6007	14959-14966	NCBITaxon:33208	denotes	animals
T6008	14981-14990	SO:0000902	denotes	transgene
T6009	15111-15118	CHEBI:60425	denotes	amyloid
T6010	15255-15262	CHEBI:60425	denotes	amyloid
T6011	15370-15373	CHEBI:50845	denotes	dox
T6012	15408-15411	PR:000004168	denotes	APP
T6013	17234-17238	NCBITaxon:10088	denotes	mice
T6014	17256-17259	CHEBI:50845	denotes	dox
T6015	17311-17314	PR:000004168	denotes	APP
T6016	17315-17319	NCBITaxon:10088	denotes	mice
T6017	17321-17328	PR:000003199	denotes	CaMKIIα
T6018	17385-17388	CHEBI:50845	denotes	dox
T6019	17429-17433	NCBITaxon:10088	denotes	mice
T6020	17463-17472	SO:0000902	denotes	transgene
T6021	17580-17583	PR:000004168	denotes	APP
T6022	17599-17608	SO:0000902	denotes	transgene
T6023	17636-17643	NCBITaxon:33208	denotes	animals
T6024	17676-17680	NCBITaxon:10088	denotes	mice
T6025	17698-17705	CHEBI:60425	denotes	amyloid
T6026	17753-17757	NCBITaxon:10088	denotes	mice
T6027	17800-17809	SO:0000902	denotes	transgene
T6028	17810-17820	GO:0010467	denotes	expression
T6029	18001-18004	CHEBI:50845	denotes	dox
T6030	18013-18020	NCBITaxon:33208	denotes	animals
T6031	18083-18089	UBERON:0000955	denotes	brains
T6032	18097-18101	NCBITaxon:10088	denotes	mice
T6033	18119-18128	SO:0000902	denotes	transgene
T6034	18171-18179	UBERON:0001851	denotes	cortical
T6035	18180-18186	UBERON:0000479	denotes	tissue
T6036	18197-18203	NCBITaxon:33208	denotes	animal
T6037	18448-18454	GO:0007601	denotes	visual
T6038	18507-18510	PR:000004168	denotes	APP
T6039	18511-18515	NCBITaxon:10088	denotes	mice
T6040	18529-18532	CHEBI:50845	denotes	dox
T6041	18682-18686	NCBITaxon:10088	denotes	mice
T6042	18795-18798	PR:000004168	denotes	APP
T6043	18799-18803	NCBITaxon:10088	denotes	mice
T6044	18881-18888	NCBITaxon:33208	denotes	animals
T6045	18942-18951	SO:0000902	denotes	transgene
T6046	19032-19038	UBERON:0000955	denotes	brains
T6047	19150-19160	GO:0010467	denotes	expression
T6048	19177-19185	UBERON:0001851	denotes	Cortical
T6049	19257-19260	CHEBI:8984	denotes	SDS
T6050	19267-19269	CHEBI:30751	denotes	FA
T6051	19294-19303	SO:0000902	denotes	transgene
T6052	19343-19348	NCBITaxon:9606	denotes	human
T6053	19377-19384	NCBITaxon:33208	denotes	animals
T6054	19395-19402	CHEBI:60425	denotes	amyloid
T6055	19481-19484	CHEBI:8984	denotes	SDS
T6056	19489-19491	CHEBI:30751	denotes	FA
T6057	19620-19623	CHEBI:8984	denotes	SDS
T6058	19628-19630	CHEBI:30751	denotes	FA
T6059	19752-19758	UBERON:0000955	denotes	brains
T6060	19882-19886	NCBITaxon:10088	denotes	mice
T6061	20227-20234	NCBITaxon:33208	denotes	animals
T6062	20291-20298	NCBITaxon:33208	denotes	animals
T6063	22471-22479	GO:0043005	denotes	neuritic
T6064	22484-22489	CL:0000125	denotes	glial
T6103	25492-25496	NCBITaxon:10088	denotes	mice
T6105	25773-25776	PR:000004168	denotes	APP
T6106	25777-25781	NCBITaxon:10088	denotes	mice
T6107	25906-25915	SO:0000902	denotes	transgene
T6108	26040-26043	PR:000004168	denotes	APP
T6109	26044-26048	NCBITaxon:10088	denotes	mice
T6110	26052-26055	CHEBI:50845	denotes	dox
T6111	26057-26064	NCBITaxon:33208	denotes	Animals
T6112	26065-26069	GO:0007567	denotes	born
T6113	26084-26087	CHEBI:50845	denotes	dox
T6114	26183-26186	CHEBI:50845	denotes	dox
T6115	26194-26201	NCBITaxon:33208	denotes	animals
T6116	26249-26266	GO:0007623	denotes	circadian rhythms
T6081	24031-24038	NCBITaxon:33208	denotes	animals
T6082	24125-24133	GO:0036268	denotes	swimming
T6083	24161-24165	NCBITaxon:10088	denotes	mice
T6084	24192-24197	CHEBI:15377	denotes	water
T6085	24218-24223	CHEBI:15377	denotes	water
T6086	24241-24245	GO:0036268	denotes	swim
T6087	24372-24379	NCBITaxon:33208	denotes	animals
T6088	24450-24454	NCBITaxon:10088	denotes	mice
T6089	24592-24599	NCBITaxon:33208	denotes	animals
T6090	24658-24662	NCBITaxon:10088	denotes	mice
T6091	24714-24721	GO:0010467	denotes	express
T6092	24864-24871	CHEBI:60425	denotes	amyloid
T6093	24881-24890	GO:0050890	denotes	cognitive
T6094	24990-24993	PR:000004168	denotes	APP
T6095	24994-24998	NCBITaxon:10088	denotes	mice
T6096	25048-25057	GO:0050890	denotes	cognitive
T6097	25074-25081	NCBITaxon:33208	denotes	animals
T6098	25131-25140	GO:0050890	denotes	cognitive
T6099	25232-25235	PR:000004168	denotes	APP
T6100	25236-25240	NCBITaxon:10088	denotes	mice
T6101	25299-25306	NCBITaxon:33208	denotes	animals
T6102	25412-25422	GO:0040011	denotes	ambulation
T6104	25660-25673	GO:0007623	denotes	diurnal cycle
T5825	28-37	SO:0000902	denotes	transgene
T6065	22605-22614	SO:0000902	denotes	transgene
T6066	22689-22697	GO:0043005	denotes	neuritic
T6067	22755-22775	CL:0002627	denotes	activated astrocytes
T6068	22794-22798	PR:000007939	denotes	GFAP
T6069	22830-22837	NCBITaxon:33208	denotes	animals
T6070	22850-22858	GO:0043005	denotes	Neuritic
T6071	22863-22868	CL:0000125	denotes	glial
T6072	22919-22923	NCBITaxon:10088	denotes	mice
T6073	22938-22947	SO:0000902	denotes	transgene
T6074	23026-23030	NCBITaxon:10088	denotes	mice
T6075	23777-23786	GO:0050890	denotes	cognitive
T6076	23808-23812	NCBITaxon:10088	denotes	mice
T6077	23839-23848	GO:0050890	denotes	cognition
T6078	23922-23931	GO:0050890	denotes	cognitive
T6079	24013-24017	NCBITaxon:10088	denotes	mice
T6080	24027-24030	PR:000004168	denotes	APP

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