PMC:1283364 / 18188-18376 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T4908","span":{"begin":0,"end":188},"obj":"sentence"},{"id":"T4909","span":{"begin":1,"end":6},"obj":"IN"},{"id":"T4910","span":{"begin":95,"end":104},"obj":"VBN"},{"id":"T4911","span":{"begin":7,"end":14},"obj":"VBG"},{"id":"T4912","span":{"begin":15,"end":18},"obj":"DT"},{"id":"T4913","span":{"begin":19,"end":24},"obj":"NNS"},{"id":"T4914","span":{"begin":25,"end":30},"obj":"CD"},{"id":"T4915","span":{"begin":31,"end":36},"obj":"NNS"},{"id":"T4916","span":{"begin":37,"end":40},"obj":"IN"},{"id":"T4917","span":{"begin":41,"end":43},"obj":"CD"},{"id":"T4918","span":{"begin":44,"end":47},"obj":"NN"},{"id":"T4919","span":{"begin":48,"end":52},"obj":"DT"},{"id":"T4920","span":{"begin":53,"end":55},"obj":"IN"},{"id":"T4921","span":{"begin":56,"end":57},"obj":"CD"},{"id":"T4922","span":{"begin":59,"end":62},"obj":"NN"},{"id":"T4923","span":{"begin":57,"end":58},"obj":"SYM"},{"id":"T4924","span":{"begin":62,"end":63},"obj":"HYPH"},{"id":"T4925","span":{"begin":63,"end":66},"obj":"CD"},{"id":"T4926","span":{"begin":66,"end":67},"obj":"NN"},{"id":"T4927","span":{"begin":68,"end":73},"obj":"NN"},{"id":"T4928","span":{"begin":73,"end":74},"obj":"HYPH"},{"id":"T4929","span":{"begin":74,"end":76},"obj":"CD"},{"id":"T4930","span":{"begin":76,"end":78},"obj":","},{"id":"T4931","span":{"begin":78,"end":81},"obj":"DT"},{"id":"T4932","span":{"begin":82,"end":90},"obj":"NN"},{"id":"T4933","span":{"begin":91,"end":94},"obj":"VBD"},{"id":"T4934","span":{"begin":105,"end":108},"obj":"IN"},{"id":"T4935","span":{"begin":109,"end":110},"obj":"CD"},{"id":"T4936","span":{"begin":111,"end":112},"obj":"NN"},{"id":"T4937","span":{"begin":113,"end":117},"obj":"IN"},{"id":"T4938","span":{"begin":118,"end":121},"obj":"NN"},{"id":"T4939","span":{"begin":122,"end":132},"obj":"VBN"},{"id":"T4940","span":{"begin":121,"end":122},"obj":"HYPH"},{"id":"T4941","span":{"begin":141,"end":142},"obj":"NN"},{"id":"T4942","span":{"begin":133,"end":140},"obj":"NN"},{"id":"T4943","span":{"begin":143,"end":144},"obj":"-LRB-"},{"id":"T4944","span":{"begin":144,"end":149},"obj":"NNP"},{"id":"T4945","span":{"begin":149,"end":150},"obj":"-RRB-"},{"id":"T4946","span":{"begin":151,"end":158},"obj":"VBN"},{"id":"T4947","span":{"begin":159,"end":160},"obj":"CD"},{"id":"T4948","span":{"begin":160,"end":161},"obj":"SYM"},{"id":"T4949","span":{"begin":161,"end":166},"obj":"CD"},{"id":"T4950","span":{"begin":167,"end":169},"obj":"IN"},{"id":"T4951","span":{"begin":170,"end":178},"obj":"NN"},{"id":"T4952","span":{"begin":179,"end":187},"obj":"NN"},{"id":"T4953","span":{"begin":187,"end":188},"obj":"."}],"relations":[{"id":"R3397","pred":"prep","subj":"T4909","obj":"T4910"},{"id":"R3398","pred":"pcomp","subj":"T4911","obj":"T4909"},{"id":"R3399","pred":"det","subj":"T4912","obj":"T4913"},{"id":"R3400","pred":"dobj","subj":"T4913","obj":"T4911"},{"id":"R3401","pred":"nummod","subj":"T4914","obj":"T4915"},{"id":"R3402","pred":"npadvmod","subj":"T4915","obj":"T4911"},{"id":"R3403","pred":"prep","subj":"T4916","obj":"T4911"},{"id":"R3404","pred":"nummod","subj":"T4917","obj":"T4918"},{"id":"R3405","pred":"pobj","subj":"T4918","obj":"T4916"},{"id":"R3406","pred":"appos","subj":"T4919","obj":"T4918"},{"id":"R3407","pred":"prep","subj":"T4920","obj":"T4911"},{"id":"R3408","pred":"nummod","subj":"T4921","obj":"T4922"},{"id":"R3409","pred":"pobj","subj":"T4922","obj":"T4920"},{"id":"R3410","pred":"punct","subj":"T4923","obj":"T4921"},{"id":"R3411","pred":"punct","subj":"T4924","obj":"T4922"},{"id":"R3412","pred":"nummod","subj":"T4925","obj":"T4926"},{"id":"R3413","pred":"compound","subj":"T4926","obj":"T4927"},{"id":"R3414","pred":"appos","subj":"T4927","obj":"T4922"},{"id":"R3415","pred":"punct","subj":"T4928","obj":"T4927"},{"id":"R3416","pred":"nummod","subj":"T4929","obj":"T4927"},{"id":"R3417","pred":"punct","subj":"T4930","obj":"T4910"},{"id":"R3418","pred":"det","subj":"T4931","obj":"T4932"},{"id":"R3419","pred":"nsubjpass","subj":"T4932","obj":"T4910"},{"id":"R3420","pred":"auxpass","subj":"T4933","obj":"T4910"},{"id":"R3421","pred":"prep","subj":"T4934","obj":"T4910"},{"id":"R3422","pred":"nummod","subj":"T4935","obj":"T4936"},{"id":"R3423","pred":"pobj","subj":"T4936","obj":"T4934"},{"id":"R3424","pred":"prep","subj":"T4937","obj":"T4910"},{"id":"R3425","pred":"npadvmod","subj":"T4938","obj":"T4939"},{"id":"R3426","pred":"amod","subj":"T4939","obj":"T4941"},{"id":"R3427","pred":"punct","subj":"T4940","obj":"T4939"},{"id":"R3428","pred":"pobj","subj":"T4941","obj":"T4937"},{"id":"R3429","pred":"compound","subj":"T4942","obj":"T4941"},{"id":"R3430","pred":"punct","subj":"T4943","obj":"T4944"},{"id":"R3431","pred":"parataxis","subj":"T4944","obj":"T4941"},{"id":"R3432","pred":"punct","subj":"T4945","obj":"T4944"},{"id":"R3433","pred":"acl","subj":"T4946","obj":"T4941"},{"id":"R3434","pred":"dobj","subj":"T4947","obj":"T4946"},{"id":"R3435","pred":"punct","subj":"T4948","obj":"T4949"},{"id":"R3436","pred":"prep","subj":"T4949","obj":"T4947"},{"id":"R3437","pred":"prep","subj":"T4950","obj":"T4946"},{"id":"R3438","pred":"compound","subj":"T4951","obj":"T4952"},{"id":"R3439","pred":"pobj","subj":"T4952","obj":"T4950"},{"id":"R3440","pred":"punct","subj":"T4953","obj":"T4910"}],"text":" After washing the blots three times for 10 min each in 1× TBS/0.1% Tween-20, the membrane was incubated for 1 h with HRP-conjugated protein A (Sigma) diluted 1:5,000 in blocking solution."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T4777","span":{"begin":68,"end":76},"obj":"CHEBI:53424"},{"id":"T4778","span":{"begin":122,"end":132},"obj":"MOP:0000779"},{"id":"T4779","span":{"begin":133,"end":140},"obj":"CHEBI_PR_EXT:protein"},{"id":"T4780","span":{"begin":133,"end":142},"obj":"PR_EXT:000029158"},{"id":"T4781","span":{"begin":179,"end":187},"obj":"CHEBI:75958"}],"text":" After washing the blots three times for 10 min each in 1× TBS/0.1% Tween-20, the membrane was incubated for 1 h with HRP-conjugated protein A (Sigma) diluted 1:5,000 in blocking solution."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T4763","span":{"begin":68,"end":76},"obj":"CHEBI:53424"},{"id":"T4764","span":{"begin":122,"end":132},"obj":"MOP:0000779"},{"id":"T4765","span":{"begin":133,"end":142},"obj":"PR:000029158"},{"id":"T4766","span":{"begin":179,"end":187},"obj":"CHEBI:75958"}],"text":" After washing the blots three times for 10 min each in 1× TBS/0.1% Tween-20, the membrane was incubated for 1 h with HRP-conjugated protein A (Sigma) diluted 1:5,000 in blocking solution."}