PMC:1283364 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T137	11-22	NN	denotes	Amyloidosis
T138	0-104	sentence	denotes	Persistent Amyloidosis following Suppression of Aβ Production in a Transgenic Model of Alzheimer Disease
T139	23-32	VBG	denotes	following
T140	33-44	NN	denotes	Suppression
T141	45-47	IN	denotes	of
T142	48-50	NN	denotes	Aβ
T143	51-61	NN	denotes	Production
T144	62-64	IN	denotes	in
T145	65-66	DT	denotes	a
T146	78-83	NN	denotes	Model
T147	67-77	JJ	denotes	Transgenic
T148	84-86	IN	denotes	of
T149	87-96	NNP	denotes	Alzheimer
T150	97-104	NN	denotes	Disease
T151	104-105	sentence	denotes
T152	154-164	sentence	denotes	Background
T153	154-164	NN	denotes	Background
T154	164-373	sentence	denotes	The proteases (secretases) that cleave amyloid-β (Aβ) peptide from the amyloid precursor protein (APP) have been the focus of considerable investigation in the development of treatments for Alzheimer disease.
T155	165-168	DT	denotes	The
T156	180-190	NNS	denotes	secretases
T157	169-178	NNS	denotes	proteases
T158	179-180	-LRB-	denotes	(
T159	273-277	VBN	denotes	been
T160	190-191	-RRB-	denotes	)
T161	192-196	WDT	denotes	that
T162	197-203	VBP	denotes	cleave
T163	204-211	NN	denotes	amyloid
T164	212-213	NN	denotes	β
T165	211-212	HYPH	denotes	-
T166	219-226	NN	denotes	peptide
T167	214-215	-LRB-	denotes	(
T168	215-217	NN	denotes	Aβ
T169	217-218	-RRB-	denotes	)
T170	227-231	IN	denotes	from
T171	232-235	DT	denotes	the
T172	254-261	NN	denotes	protein
T173	236-243	NN	denotes	amyloid
T174	244-253	NN	denotes	precursor
T175	262-263	-LRB-	denotes	(
T176	263-266	NN	denotes	APP
T177	266-267	-RRB-	denotes	)
T178	268-272	VBP	denotes	have
T179	278-281	DT	denotes	the
T180	282-287	NN	denotes	focus
T181	288-290	IN	denotes	of
T182	291-303	JJ	denotes	considerable
T183	304-317	NN	denotes	investigation
T184	318-320	IN	denotes	in
T185	321-324	DT	denotes	the
T186	325-336	NN	denotes	development
T187	337-339	IN	denotes	of
T188	340-350	NNS	denotes	treatments
T189	351-354	IN	denotes	for
T190	355-364	NNP	denotes	Alzheimer
T191	365-372	NN	denotes	disease
T192	372-373	.	denotes	.
T193	373-603	sentence	denotes	The prediction has been that reducing Aβ production in the brain, even after the onset of clinical symptoms and the development of associated pathology, will facilitate the repair of damaged tissue and removal of amyloid lesions.
T194	374-377	DT	denotes	The
T195	378-388	NN	denotes	prediction
T196	393-397	VBN	denotes	been
T197	389-392	VBZ	denotes	has
T198	398-402	IN	denotes	that
T199	532-542	VB	denotes	facilitate
T200	403-411	VBG	denotes	reducing
T201	412-414	NN	denotes	Aβ
T202	415-425	NN	denotes	production
T203	426-428	IN	denotes	in
T204	429-432	DT	denotes	the
T205	433-438	NN	denotes	brain
T206	438-440	,	denotes	,
T207	440-444	RB	denotes	even
T208	445-450	IN	denotes	after
T209	451-454	DT	denotes	the
T210	455-460	NN	denotes	onset
T211	461-463	IN	denotes	of
T212	464-472	JJ	denotes	clinical
T213	473-481	NNS	denotes	symptoms
T214	482-485	CC	denotes	and
T215	486-489	DT	denotes	the
T216	490-501	NN	denotes	development
T217	502-504	IN	denotes	of
T218	505-515	VBN	denotes	associated
T219	516-525	NN	denotes	pathology
T220	525-527	,	denotes	,
T221	527-531	MD	denotes	will
T222	543-546	DT	denotes	the
T223	547-553	NN	denotes	repair
T224	554-556	IN	denotes	of
T225	557-564	VBN	denotes	damaged
T226	565-571	NN	denotes	tissue
T227	572-575	CC	denotes	and
T228	576-583	NN	denotes	removal
T229	584-586	IN	denotes	of
T230	587-594	NN	denotes	amyloid
T231	595-602	NNS	denotes	lesions
T232	602-603	.	denotes	.
T233	603-723	sentence	denotes	However, no long-term studies using animal models of amyloid pathology have yet been performed to test this hypothesis.
T234	604-611	RB	denotes	However
T235	689-698	VBN	denotes	performed
T236	611-613	,	denotes	,
T237	613-615	DT	denotes	no
T238	626-633	NNS	denotes	studies
T239	616-620	JJ	denotes	long
T240	621-625	NN	denotes	term
T241	620-621	HYPH	denotes	-
T242	634-639	VBG	denotes	using
T243	640-646	NN	denotes	animal
T244	647-653	NNS	denotes	models
T245	654-656	IN	denotes	of
T246	657-664	NN	denotes	amyloid
T247	665-674	NN	denotes	pathology
T248	675-679	VBP	denotes	have
T249	680-683	RB	denotes	yet
T250	684-688	VBN	denotes	been
T251	699-701	TO	denotes	to
T252	702-706	VB	denotes	test
T253	707-711	DT	denotes	this
T254	712-722	NN	denotes	hypothesis
T255	722-723	.	denotes	.
T256	723-745	sentence	denotes	Methods and Findings
T257	725-732	NNS	denotes	Methods
T258	733-736	CC	denotes	and
T259	737-745	NNS	denotes	Findings
T260	745-891	sentence	denotes	We have generated a transgenic mouse model that genetically mimics the arrest of Aβ production expected from treatment with secretase inhibitors.
T261	746-748	PRP	denotes	We
T262	754-763	VBN	denotes	generated
T263	749-753	VBP	denotes	have
T264	764-765	DT	denotes	a
T265	783-788	NN	denotes	model
T266	766-776	JJ	denotes	transgenic
T267	777-782	NN	denotes	mouse
T268	789-793	WDT	denotes	that
T269	806-812	VBZ	denotes	mimics
T270	794-805	RB	denotes	genetically
T271	813-816	DT	denotes	the
T272	817-823	NN	denotes	arrest
T273	824-826	IN	denotes	of
T274	827-829	NN	denotes	Aβ
T275	830-840	NN	denotes	production
T276	841-849	VBN	denotes	expected
T277	850-854	IN	denotes	from
T278	855-864	NN	denotes	treatment
T279	865-869	IN	denotes	with
T280	870-879	NN	denotes	secretase
T281	880-890	NNS	denotes	inhibitors
T282	890-891	.	denotes	.
T283	891-977	sentence	denotes	These mice overexpress mutant APP from a vector that can be regulated by doxycycline.
T284	892-897	DT	denotes	These
T285	898-902	NNS	denotes	mice
T286	903-914	VBP	denotes	overexpress
T287	915-921	NN	denotes	mutant
T288	922-925	NN	denotes	APP
T289	926-930	IN	denotes	from
T290	931-932	DT	denotes	a
T291	933-939	NN	denotes	vector
T292	940-944	WDT	denotes	that
T293	952-961	VBN	denotes	regulated
T294	945-948	MD	denotes	can
T295	949-951	VB	denotes	be
T296	962-964	IN	denotes	by
T297	965-976	NN	denotes	doxycycline
T298	976-977	.	denotes	.
T299	977-1076	sentence	denotes	Under normal conditions, high-level expression of APP quickly induces fulminant amyloid pathology.
T300	978-983	IN	denotes	Under
T301	1040-1047	VBZ	denotes	induces
T302	984-990	JJ	denotes	normal
T303	991-1001	NNS	denotes	conditions
T304	1001-1003	,	denotes	,
T305	1003-1007	JJ	denotes	high
T306	1008-1013	NN	denotes	level
T307	1007-1008	HYPH	denotes	-
T308	1014-1024	NN	denotes	expression
T309	1025-1027	IN	denotes	of
T310	1028-1031	NN	denotes	APP
T311	1032-1039	RB	denotes	quickly
T312	1048-1057	JJ	denotes	fulminant
T313	1066-1075	NN	denotes	pathology
T314	1058-1065	NN	denotes	amyloid
T315	1075-1076	.	denotes	.
T316	1076-1236	sentence	denotes	We show that doxycycline administration inhibits transgenic APP expression by greater than 95% and reduces Aβ production to levels found in nontransgenic mice.
T317	1077-1079	PRP	denotes	We
T318	1080-1084	VBP	denotes	show
T319	1085-1089	IN	denotes	that
T320	1117-1125	VBZ	denotes	inhibits
T321	1090-1101	NN	denotes	doxycycline
T322	1102-1116	NN	denotes	administration
T323	1126-1136	JJ	denotes	transgenic
T324	1141-1151	NN	denotes	expression
T325	1137-1140	NN	denotes	APP
T326	1152-1154	IN	denotes	by
T327	1155-1162	JJR	denotes	greater
T328	1168-1170	CD	denotes	95
T329	1163-1167	IN	denotes	than
T330	1170-1171	NN	denotes	%
T331	1172-1175	CC	denotes	and
T332	1176-1183	VBZ	denotes	reduces
T333	1184-1186	NN	denotes	Aβ
T334	1187-1197	NN	denotes	production
T335	1198-1200	IN	denotes	to
T336	1201-1207	NNS	denotes	levels
T337	1208-1213	VBN	denotes	found
T338	1214-1216	IN	denotes	in
T339	1217-1230	JJ	denotes	nontransgenic
T340	1231-1235	NNS	denotes	mice
T341	1235-1236	.	denotes	.
T342	1236-1342	sentence	denotes	Suppression of transgenic Aβ synthesis in this model abruptly halts the progression of amyloid pathology.
T343	1237-1248	NN	denotes	Suppression
T344	1299-1304	VBZ	denotes	halts
T345	1249-1251	IN	denotes	of
T346	1252-1262	JJ	denotes	transgenic
T347	1266-1275	NN	denotes	synthesis
T348	1263-1265	NN	denotes	Aβ
T349	1276-1278	IN	denotes	in
T350	1279-1283	DT	denotes	this
T351	1284-1289	NN	denotes	model
T352	1290-1298	RB	denotes	abruptly
T353	1305-1308	DT	denotes	the
T354	1309-1320	NN	denotes	progression
T355	1321-1323	IN	denotes	of
T356	1324-1331	NN	denotes	amyloid
T357	1332-1341	NN	denotes	pathology
T358	1341-1342	.	denotes	.
T359	1342-1495	sentence	denotes	However, formation and disaggregation of amyloid deposits appear to be in disequilibrium as the plaques require far longer to disperse than to assemble.
T360	1343-1350	RB	denotes	However
T361	1401-1407	VBP	denotes	appear
T362	1350-1352	,	denotes	,
T363	1352-1361	NN	denotes	formation
T364	1362-1365	CC	denotes	and
T365	1366-1380	NN	denotes	disaggregation
T366	1381-1383	IN	denotes	of
T367	1384-1391	NN	denotes	amyloid
T368	1392-1400	NNS	denotes	deposits
T369	1408-1410	TO	denotes	to
T370	1411-1413	VB	denotes	be
T371	1414-1416	IN	denotes	in
T372	1417-1431	NN	denotes	disequilibrium
T373	1432-1434	IN	denotes	as
T374	1447-1454	VBP	denotes	require
T375	1435-1438	DT	denotes	the
T376	1439-1446	NNS	denotes	plaques
T377	1455-1458	RB	denotes	far
T378	1459-1465	RBR	denotes	longer
T379	1466-1468	TO	denotes	to
T380	1469-1477	VB	denotes	disperse
T381	1478-1482	IN	denotes	than
T382	1486-1494	VB	denotes	assemble
T383	1483-1485	TO	denotes	to
T384	1494-1495	.	denotes	.
T385	1495-1667	sentence	denotes	Mice in which APP synthesis was suppressed for as long as 6 mo after the formation of Aβ deposits retain a considerable amyloid load, with little sign of active clearance.
T386	1496-1500	NNS	denotes	Mice
T387	1594-1600	VBP	denotes	retain
T388	1501-1503	IN	denotes	in
T389	1528-1538	VBN	denotes	suppressed
T390	1504-1509	WDT	denotes	which
T391	1510-1513	NN	denotes	APP
T392	1514-1523	NN	denotes	synthesis
T393	1524-1527	VBD	denotes	was
T394	1539-1542	IN	denotes	for
T395	1543-1545	RB	denotes	as
T396	1554-1555	CD	denotes	6
T397	1546-1550	JJ	denotes	long
T398	1551-1553	IN	denotes	as
T399	1556-1558	NN	denotes	mo
T400	1559-1564	IN	denotes	after
T401	1565-1568	DT	denotes	the
T402	1569-1578	NN	denotes	formation
T403	1579-1581	IN	denotes	of
T404	1582-1584	NN	denotes	Aβ
T405	1585-1593	NNS	denotes	deposits
T406	1601-1602	DT	denotes	a
T407	1624-1628	NN	denotes	load
T408	1603-1615	JJ	denotes	considerable
T409	1616-1623	NN	denotes	amyloid
T410	1628-1630	,	denotes	,
T411	1630-1634	IN	denotes	with
T412	1635-1641	JJ	denotes	little
T413	1642-1646	NN	denotes	sign
T414	1647-1649	IN	denotes	of
T415	1650-1656	JJ	denotes	active
T416	1657-1666	NN	denotes	clearance
T417	1666-1667	.	denotes	.
T418	1667-1679	sentence	denotes	Conclusion
T419	1669-1679	NN	denotes	Conclusion
T420	1679-1811	sentence	denotes	This study demonstrates that amyloid lesions in transgenic mice are highly stable structures in vivo that are slow to disaggregate.
T421	1680-1684	DT	denotes	This
T422	1685-1690	NN	denotes	study
T423	1691-1703	VBZ	denotes	demonstrates
T424	1704-1708	IN	denotes	that
T425	1744-1747	VBP	denotes	are
T426	1709-1716	NN	denotes	amyloid
T427	1717-1724	NNS	denotes	lesions
T428	1725-1727	IN	denotes	in
T429	1728-1738	JJ	denotes	transgenic
T430	1739-1743	NNS	denotes	mice
T431	1748-1754	RB	denotes	highly
T432	1755-1761	JJ	denotes	stable
T433	1762-1772	NNS	denotes	structures
T434	1773-1775	FW	denotes	in
T435	1776-1780	FW	denotes	vivo
T436	1781-1785	WDT	denotes	that
T437	1786-1789	VBP	denotes	are
T438	1790-1794	JJ	denotes	slow
T439	1795-1797	TO	denotes	to
T440	1798-1810	VB	denotes	disaggregate
T441	1810-1811	.	denotes	.
T442	1811-2079	sentence	denotes	Our findings suggest that arresting Aβ production in patients with Alzheimer disease should halt the progression of pathology, but that early treatment may be imperative, as it appears that amyloid deposits, once formed, will require additional intervention to clear.
T443	1812-1815	PRP$	denotes	Our
T444	1816-1824	NNS	denotes	findings
T445	1825-1832	VBP	denotes	suggest
T446	1833-1837	IN	denotes	that
T447	1904-1908	VB	denotes	halt
T448	1838-1847	VBG	denotes	arresting
T449	1848-1850	NN	denotes	Aβ
T450	1851-1861	NN	denotes	production
T451	1862-1864	IN	denotes	in
T452	1865-1873	NNS	denotes	patients
T453	1874-1878	IN	denotes	with
T454	1879-1888	NNP	denotes	Alzheimer
T455	1889-1896	NN	denotes	disease
T456	1897-1903	MD	denotes	should
T457	1909-1912	DT	denotes	the
T458	1913-1924	NN	denotes	progression
T459	1925-1927	IN	denotes	of
T460	1928-1937	NN	denotes	pathology
T461	1937-1939	,	denotes	,
T462	1939-1942	CC	denotes	but
T463	1943-1947	IN	denotes	that
T464	1968-1970	VB	denotes	be
T465	1948-1953	JJ	denotes	early
T466	1954-1963	NN	denotes	treatment
T467	1964-1967	MD	denotes	may
T468	1971-1981	JJ	denotes	imperative
T469	1981-1983	,	denotes	,
T470	1983-1985	IN	denotes	as
T471	1989-1996	VBZ	denotes	appears
T472	1986-1988	PRP	denotes	it
T473	1997-2001	IN	denotes	that
T474	2038-2045	VB	denotes	require
T475	2002-2009	NN	denotes	amyloid
T476	2010-2018	NNS	denotes	deposits
T477	2018-2020	,	denotes	,
T478	2020-2024	IN	denotes	once
T479	2025-2031	VBN	denotes	formed
T480	2031-2033	,	denotes	,
T481	2033-2037	MD	denotes	will
T482	2046-2056	JJ	denotes	additional
T483	2057-2069	NN	denotes	intervention
T484	2070-2072	TO	denotes	to
T485	2073-2078	VB	denotes	clear
T486	2078-2079	.	denotes	.
T706	2264-2268	IN	denotes	Over
T707	2269-2270	DT	denotes	a
T708	2271-2277	NN	denotes	decade
T709	2278-2281	RB	denotes	ago
T710	2313-2322	VBD	denotes	predicted
T711	2282-2285	DT	denotes	the
T712	2302-2312	NN	denotes	hypothesis
T713	2286-2293	NN	denotes	amyloid
T714	2294-2301	NN	denotes	cascade
T715	2323-2327	IN	denotes	that
T716	2371-2375	VBP	denotes	lead
T717	2328-2337	VBN	denotes	increased
T718	2338-2344	NNS	denotes	levels
T719	2345-2347	IN	denotes	of
T720	2348-2355	NN	denotes	amyloid
T721	2356-2357	NN	denotes	β
T722	2355-2356	HYPH	denotes	-
T723	2363-2370	NN	denotes	peptide
T724	2358-2359	-LRB-	denotes	(
T725	2359-2361	NN	denotes	Aβ
T726	2361-2362	-RRB-	denotes	)
T727	2376-2378	IN	denotes	to
T728	2379-2388	JJ	denotes	secondary
T729	2389-2400	NNS	denotes	pathologies
T730	2401-2405	WDT	denotes	that
T731	2417-2426	VBP	denotes	culminate
T732	2406-2416	RB	denotes	ultimately
T733	2427-2429	IN	denotes	in
T734	2430-2433	DT	denotes	the
T735	2434-2439	NN	denotes	onset
T736	2440-2442	IN	denotes	of
T737	2443-2452	NNP	denotes	Alzheimer
T738	2453-2460	NN	denotes	disease
T739	2461-2462	-LRB-	denotes	(
T740	2462-2464	NN	denotes	AD
T741	2464-2465	-RRB-	denotes	)
T742	2466-2467	-LRB-	denotes	[
T743	2467-2468	CD	denotes	1
T744	2468-2469	-RRB-	denotes	]
T745	2469-2470	.	denotes	.
T746	2470-2770	sentence	denotes	Early support for this hypothesis came from genetic studies linking early-onset AD to mutations in the amyloid precursor protein (APP), from which Aβ is derived, and presenilins 1 and 2, which are interchangeable components of a endoprotease complex that releases Aβ from APP (for review see [2,3]).
T747	2471-2476	JJ	denotes	Early
T748	2477-2484	NN	denotes	support
T749	2505-2509	VBD	denotes	came
T750	2485-2488	IN	denotes	for
T751	2489-2493	DT	denotes	this
T752	2494-2504	NN	denotes	hypothesis
T753	2510-2514	IN	denotes	from
T754	2515-2522	JJ	denotes	genetic
T755	2523-2530	NNS	denotes	studies
T756	2531-2538	VBG	denotes	linking
T757	2539-2544	JJ	denotes	early
T758	2545-2550	NN	denotes	onset
T759	2544-2545	HYPH	denotes	-
T760	2551-2553	NN	denotes	AD
T761	2554-2556	IN	denotes	to
T762	2557-2566	NNS	denotes	mutations
T763	2567-2569	IN	denotes	in
T764	2570-2573	DT	denotes	the
T765	2592-2599	NN	denotes	protein
T766	2574-2581	NN	denotes	amyloid
T767	2582-2591	NN	denotes	precursor
T768	2600-2601	-LRB-	denotes	(
T769	2601-2604	NN	denotes	APP
T770	2604-2605	-RRB-	denotes	)
T771	2605-2607	,	denotes	,
T772	2607-2611	IN	denotes	from
T773	2624-2631	VBN	denotes	derived
T774	2612-2617	WDT	denotes	which
T775	2618-2620	NN	denotes	Aβ
T776	2621-2623	VBZ	denotes	is
T777	2631-2633	,	denotes	,
T778	2633-2636	CC	denotes	and
T779	2637-2648	NNS	denotes	presenilins
T780	2649-2650	CD	denotes	1
T781	2651-2654	CC	denotes	and
T782	2655-2656	CD	denotes	2
T783	2656-2658	,	denotes	,
T784	2658-2663	WDT	denotes	which
T785	2664-2667	VBP	denotes	are
T786	2668-2683	JJ	denotes	interchangeable
T787	2684-2694	NNS	denotes	components
T788	2695-2697	IN	denotes	of
T789	2698-2699	DT	denotes	a
T790	2713-2720	NN	denotes	complex
T791	2700-2712	NN	denotes	endoprotease
T792	2721-2725	WDT	denotes	that
T793	2726-2734	VBZ	denotes	releases
T794	2735-2737	NN	denotes	Aβ
T795	2738-2742	IN	denotes	from
T796	2743-2746	NN	denotes	APP
T797	2747-2748	-LRB-	denotes	(
T798	2759-2762	VBP	denotes	see
T799	2748-2751	IN	denotes	for
T800	2752-2758	NN	denotes	review
T801	2763-2764	-LRB-	denotes	[
T802	2764-2765	CD	denotes	2
T803	2766-2767	CD	denotes	3
T804	2765-2766	,	denotes	,
T805	2767-2768	-RRB-	denotes	]
T806	2768-2769	-RRB-	denotes	)
T807	2769-2770	.	denotes	.
T808	2770-2984	sentence	denotes	If, as predicted, overproduction of Aβ initiates the cascade of events leading to disease, then therapeutic strategies that lower Aβ levels should either arrest or reverse the progression from peptide to dementia.
T809	2771-2773	IN	denotes	If
T810	2810-2819	VBZ	denotes	initiates
T811	2773-2775	,	denotes	,
T812	2775-2777	IN	denotes	as
T813	2778-2787	VBN	denotes	predicted
T814	2787-2789	,	denotes	,
T815	2789-2803	NN	denotes	overproduction
T816	2804-2806	IN	denotes	of
T817	2807-2809	NN	denotes	Aβ
T818	2925-2931	VB	denotes	arrest
T819	2820-2823	DT	denotes	the
T820	2824-2831	NN	denotes	cascade
T821	2832-2834	IN	denotes	of
T822	2835-2841	NNS	denotes	events
T823	2842-2849	VBG	denotes	leading
T824	2850-2852	IN	denotes	to
T825	2853-2860	NN	denotes	disease
T826	2860-2862	,	denotes	,
T827	2862-2866	RB	denotes	then
T828	2867-2878	JJ	denotes	therapeutic
T829	2879-2889	NNS	denotes	strategies
T830	2890-2894	WDT	denotes	that
T831	2895-2900	VBP	denotes	lower
T832	2901-2903	NN	denotes	Aβ
T833	2904-2910	NNS	denotes	levels
T834	2911-2917	MD	denotes	should
T835	2918-2924	CC	denotes	either
T836	2932-2934	CC	denotes	or
T837	2935-2942	VB	denotes	reverse
T838	2943-2946	DT	denotes	the
T839	2947-2958	NN	denotes	progression
T840	2959-2963	IN	denotes	from
T841	2964-2971	NN	denotes	peptide
T842	2972-2974	IN	denotes	to
T843	2975-2983	NN	denotes	dementia
T844	2983-2984	.	denotes	.
T845	2984-3196	sentence	denotes	Early evidence from clinical trials of antibody-mediated clearance, one of the first Aβ-lowering approaches tested in humans, suggested that treatments designed to reduce amyloid burden may indeed be beneficial.
T846	2985-2990	JJ	denotes	Early
T847	2991-2999	NN	denotes	evidence
T848	3111-3120	VBD	denotes	suggested
T849	3000-3004	IN	denotes	from
T850	3005-3013	JJ	denotes	clinical
T851	3014-3020	NNS	denotes	trials
T852	3021-3023	IN	denotes	of
T853	3024-3032	NN	denotes	antibody
T854	3033-3041	VBN	denotes	mediated
T855	3032-3033	HYPH	denotes	-
T856	3042-3051	NN	denotes	clearance
T857	3051-3053	,	denotes	,
T858	3053-3056	CD	denotes	one
T859	3057-3059	IN	denotes	of
T860	3060-3063	DT	denotes	the
T861	3082-3092	NNS	denotes	approaches
T862	3064-3069	JJ	denotes	first
T863	3070-3072	NN	denotes	Aβ
T864	3073-3081	VBG	denotes	lowering
T865	3072-3073	HYPH	denotes	-
T866	3093-3099	VBN	denotes	tested
T867	3100-3102	IN	denotes	in
T868	3103-3109	NNS	denotes	humans
T869	3109-3111	,	denotes	,
T870	3121-3125	IN	denotes	that
T871	3182-3184	VB	denotes	be
T872	3126-3136	NNS	denotes	treatments
T873	3137-3145	VBN	denotes	designed
T874	3146-3148	TO	denotes	to
T875	3149-3155	VB	denotes	reduce
T876	3156-3163	NN	denotes	amyloid
T877	3164-3170	NN	denotes	burden
T878	3171-3174	MD	denotes	may
T879	3175-3181	RB	denotes	indeed
T880	3185-3195	JJ	denotes	beneficial
T881	3195-3196	.	denotes	.
T882	3196-3488	sentence	denotes	Although the trials were halted because of adverse effects in a subset of volunteers [4,5], further analysis of several patients found evidence that amyloid pathology, and to a lesser degree cognitive function, was improved in proportion to the patient's titer of Aβ-specific antibody [6,7].
T883	3197-3205	IN	denotes	Although
T884	3222-3228	VBN	denotes	halted
T885	3206-3209	DT	denotes	the
T886	3210-3216	NNS	denotes	trials
T887	3217-3221	VBD	denotes	were
T888	3326-3331	VBD	denotes	found
T889	3229-3236	IN	denotes	because
T890	3237-3239	IN	denotes	of
T891	3240-3247	JJ	denotes	adverse
T892	3248-3255	NNS	denotes	effects
T893	3256-3258	IN	denotes	in
T894	3259-3260	DT	denotes	a
T895	3261-3267	NN	denotes	subset
T896	3268-3270	IN	denotes	of
T897	3271-3281	NNS	denotes	volunteers
T898	3282-3283	-LRB-	denotes	[
T899	3285-3286	CD	denotes	5
T900	3283-3284	CD	denotes	4
T901	3284-3285	,	denotes	,
T902	3286-3287	-RRB-	denotes	]
T903	3287-3289	,	denotes	,
T904	3289-3296	JJ	denotes	further
T905	3297-3305	NN	denotes	analysis
T906	3306-3308	IN	denotes	of
T907	3309-3316	JJ	denotes	several
T908	3317-3325	NNS	denotes	patients
T909	3332-3340	NN	denotes	evidence
T910	3341-3345	IN	denotes	that
T911	3412-3420	VBN	denotes	improved
T912	3346-3353	NN	denotes	amyloid
T913	3354-3363	NN	denotes	pathology
T914	3363-3365	,	denotes	,
T915	3365-3368	CC	denotes	and
T916	3369-3371	IN	denotes	to
T917	3398-3406	NN	denotes	function
T918	3372-3373	DT	denotes	a
T919	3381-3387	NN	denotes	degree
T920	3374-3380	JJR	denotes	lesser
T921	3388-3397	JJ	denotes	cognitive
T922	3406-3408	,	denotes	,
T923	3408-3411	VBD	denotes	was
T924	3421-3423	IN	denotes	in
T925	3424-3434	NN	denotes	proportion
T926	3435-3437	IN	denotes	to
T927	3438-3441	DT	denotes	the
T928	3442-3449	NN	denotes	patient
T929	3452-3457	NN	denotes	titer
T930	3449-3451	POS	denotes	's
T931	3458-3460	IN	denotes	of
T932	3461-3463	NN	denotes	Aβ
T933	3464-3472	JJ	denotes	specific
T934	3463-3464	HYPH	denotes	-
T935	3473-3481	NN	denotes	antibody
T936	3482-3483	-LRB-	denotes	[
T937	3485-3486	CD	denotes	7
T938	3483-3484	CD	denotes	6
T939	3484-3485	,	denotes	,
T940	3486-3487	-RRB-	denotes	]
T941	3487-3488	.	denotes	.
T942	3488-3677	sentence	denotes	While this approach is promising, constant exposure to antibodies that recognize an epitope highly enriched in the brain may have unexpected side effects that will limit its long-term use.
T943	3489-3494	IN	denotes	While
T944	3509-3511	VBZ	denotes	is
T945	3495-3499	DT	denotes	this
T946	3500-3508	NN	denotes	approach
T947	3614-3618	VB	denotes	have
T948	3512-3521	JJ	denotes	promising
T949	3521-3523	,	denotes	,
T950	3523-3531	JJ	denotes	constant
T951	3532-3540	NN	denotes	exposure
T952	3541-3543	IN	denotes	to
T953	3544-3554	NNS	denotes	antibodies
T954	3555-3559	WDT	denotes	that
T955	3560-3569	VBP	denotes	recognize
T956	3570-3572	DT	denotes	an
T957	3573-3580	NN	denotes	epitope
T958	3581-3587	RB	denotes	highly
T959	3588-3596	VBN	denotes	enriched
T960	3597-3599	IN	denotes	in
T961	3600-3603	DT	denotes	the
T962	3604-3609	NN	denotes	brain
T963	3610-3613	MD	denotes	may
T964	3619-3629	JJ	denotes	unexpected
T965	3635-3642	NNS	denotes	effects
T966	3630-3634	NN	denotes	side
T967	3643-3647	WDT	denotes	that
T968	3653-3658	VB	denotes	limit
T969	3648-3652	MD	denotes	will
T970	3659-3662	PRP$	denotes	its
T971	3673-3676	NN	denotes	use
T972	3663-3667	JJ	denotes	long
T973	3668-3672	NN	denotes	term
T974	3667-3668	HYPH	denotes	-
T975	3676-3677	.	denotes	.
T976	3677-3843	sentence	denotes	An alternative approach that is being actively pursued for future treatment of AD seeks to lower Aβ levels by limiting its production from the precursor protein APP.
T977	3678-3680	DT	denotes	An
T978	3693-3701	NN	denotes	approach
T979	3681-3692	JJ	denotes	alternative
T980	3760-3765	VBZ	denotes	seeks
T981	3702-3706	WDT	denotes	that
T982	3725-3732	VBN	denotes	pursued
T983	3707-3709	VBZ	denotes	is
T984	3710-3715	VBG	denotes	being
T985	3716-3724	RB	denotes	actively
T986	3733-3736	IN	denotes	for
T987	3737-3743	JJ	denotes	future
T988	3744-3753	NN	denotes	treatment
T989	3754-3756	IN	denotes	of
T990	3757-3759	NN	denotes	AD
T991	3766-3768	TO	denotes	to
T992	3769-3774	VB	denotes	lower
T993	3775-3777	NN	denotes	Aβ
T994	3778-3784	NNS	denotes	levels
T995	3785-3787	IN	denotes	by
T996	3788-3796	VBG	denotes	limiting
T997	3797-3800	PRP$	denotes	its
T998	3801-3811	NN	denotes	production
T999	3812-3816	IN	denotes	from
T1000	3817-3820	DT	denotes	the
T1001	3831-3838	NN	denotes	protein
T1002	3821-3830	NN	denotes	precursor
T1003	3839-3842	NN	denotes	APP
T1004	3842-3843	.	denotes	.
T1005	3843-4032	sentence	denotes	Peptide Aβ is released from APP by the action of two enzymes, the β-APP cleaving enzyme 1 (BACE1) and γ-secretase, which cleave the holoprotein at the N- and C-termini of Aβ, respectively.
T1006	3844-3851	NN	denotes	Peptide
T1007	3852-3854	NN	denotes	Aβ
T1008	3858-3866	VBN	denotes	released
T1009	3855-3857	VBZ	denotes	is
T1010	3867-3871	IN	denotes	from
T1011	3872-3875	NN	denotes	APP
T1012	3876-3878	IN	denotes	by
T1013	3879-3882	DT	denotes	the
T1014	3883-3889	NN	denotes	action
T1015	3890-3892	IN	denotes	of
T1016	3893-3896	CD	denotes	two
T1017	3897-3904	NNS	denotes	enzymes
T1018	3904-3906	,	denotes	,
T1019	3906-3909	DT	denotes	the
T1020	3925-3931	NN	denotes	enzyme
T1021	3910-3911	NN	denotes	β
T1022	3912-3915	NN	denotes	APP
T1023	3911-3912	HYPH	denotes	-
T1024	3916-3924	VBG	denotes	cleaving
T1025	3932-3933	CD	denotes	1
T1026	3934-3935	-LRB-	denotes	(
T1027	3935-3940	NN	denotes	BACE1
T1028	3940-3941	-RRB-	denotes	)
T1029	3942-3945	CC	denotes	and
T1030	3946-3947	SYM	denotes	γ
T1031	3948-3957	NN	denotes	secretase
T1032	3947-3948	HYPH	denotes	-
T1033	3957-3959	,	denotes	,
T1034	3959-3964	WDT	denotes	which
T1035	3965-3971	VBP	denotes	cleave
T1036	3972-3975	DT	denotes	the
T1037	3976-3987	NN	denotes	holoprotein
T1038	3988-3990	IN	denotes	at
T1039	3991-3994	DT	denotes	the
T1040	4004-4011	NNS	denotes	termini
T1041	3995-3996	NN	denotes	N
T1042	3996-3997	HYPH	denotes	-
T1043	3998-4001	CC	denotes	and
T1044	4002-4003	NN	denotes	C
T1045	4003-4004	HYPH	denotes	-
T1046	4012-4014	IN	denotes	of
T1047	4015-4017	NN	denotes	Aβ
T1048	4017-4019	,	denotes	,
T1049	4019-4031	RB	denotes	respectively
T1050	4031-4032	.	denotes	.
T1051	4032-4196	sentence	denotes	Several inhibitors of γ-secretase have already been produced [8,9], and small molecule inhibitors of β-APP cleaving enzyme 1 are currently being developed [10,11].
T1052	4033-4040	JJ	denotes	Several
T1053	4041-4051	NNS	denotes	inhibitors
T1054	4085-4093	VBN	denotes	produced
T1055	4052-4054	IN	denotes	of
T1056	4055-4056	SYM	denotes	γ
T1057	4057-4066	NN	denotes	secretase
T1058	4056-4057	HYPH	denotes	-
T1059	4067-4071	VBP	denotes	have
T1060	4072-4079	RB	denotes	already
T1061	4080-4084	VBN	denotes	been
T1062	4094-4095	-LRB-	denotes	[
T1063	4097-4098	CD	denotes	9
T1064	4095-4096	CD	denotes	8
T1065	4096-4097	,	denotes	,
T1066	4098-4099	-RRB-	denotes	]
T1067	4099-4101	,	denotes	,
T1068	4101-4104	CC	denotes	and
T1069	4105-4110	JJ	denotes	small
T1070	4120-4130	NNS	denotes	inhibitors
T1071	4111-4119	NN	denotes	molecule
T1072	4178-4187	VBN	denotes	developed
T1073	4131-4133	IN	denotes	of
T1074	4134-4135	NN	denotes	β
T1075	4136-4139	NN	denotes	APP
T1076	4135-4136	HYPH	denotes	-
T1077	4140-4148	VBG	denotes	cleaving
T1078	4149-4155	NN	denotes	enzyme
T1079	4156-4157	CD	denotes	1
T1080	4158-4161	VBP	denotes	are
T1081	4162-4171	RB	denotes	currently
T1082	4172-4177	VBG	denotes	being
T1083	4188-4189	-LRB-	denotes	[
T1084	4192-4194	CD	denotes	11
T1085	4189-4191	CD	denotes	10
T1086	4191-4192	,	denotes	,
T1087	4194-4195	-RRB-	denotes	]
T1088	4195-4196	.	denotes	.
T1089	4196-4308	sentence	denotes	The long-term effectiveness of this approach in either humans or model systems, however, has not been reported.
T1090	4197-4200	DT	denotes	The
T1091	4211-4224	NN	denotes	effectiveness
T1092	4201-4205	JJ	denotes	long
T1093	4206-4210	NN	denotes	term
T1094	4205-4206	HYPH	denotes	-
T1095	4299-4307	VBN	denotes	reported
T1096	4225-4227	IN	denotes	of
T1097	4228-4232	DT	denotes	this
T1098	4233-4241	NN	denotes	approach
T1099	4242-4244	IN	denotes	in
T1100	4245-4251	CC	denotes	either
T1101	4252-4258	NNS	denotes	humans
T1102	4259-4261	CC	denotes	or
T1103	4262-4267	NN	denotes	model
T1104	4268-4275	NNS	denotes	systems
T1105	4275-4277	,	denotes	,
T1106	4277-4284	RB	denotes	however
T1107	4284-4286	,	denotes	,
T1108	4286-4289	VBZ	denotes	has
T1109	4290-4293	RB	denotes	not
T1110	4294-4298	VBN	denotes	been
T1111	4307-4308	.	denotes	.
T1112	4308-4604	sentence	denotes	Although loss of β-APP cleaving enzyme 1 function can prevent the development of plaques in transgenic mouse models for AD (F. Laird, H. Cai, P. C. Wong, personal communication), it is not known whether the brain can clear pre-existing amyloid deposits once production of Aβ has been suppressed.
T1113	4309-4317	IN	denotes	Although
T1114	4363-4370	VB	denotes	prevent
T1115	4318-4322	NN	denotes	loss
T1116	4323-4325	IN	denotes	of
T1117	4326-4327	NN	denotes	β
T1118	4328-4331	NN	denotes	APP
T1119	4327-4328	HYPH	denotes	-
T1120	4332-4340	VBG	denotes	cleaving
T1121	4350-4358	NN	denotes	function
T1122	4341-4347	NN	denotes	enzyme
T1123	4348-4349	CD	denotes	1
T1124	4359-4362	MD	denotes	can
T1125	4498-4503	VBN	denotes	known
T1126	4371-4374	DT	denotes	the
T1127	4375-4386	NN	denotes	development
T1128	4387-4389	IN	denotes	of
T1129	4390-4397	NNS	denotes	plaques
T1130	4398-4400	IN	denotes	in
T1131	4401-4411	JJ	denotes	transgenic
T1132	4418-4424	NNS	denotes	models
T1133	4412-4417	NN	denotes	mouse
T1134	4425-4428	IN	denotes	for
T1135	4429-4431	NN	denotes	AD
T1136	4432-4433	-LRB-	denotes	(
T1137	4433-4435	NNP	denotes	F.
T1138	4436-4441	NNP	denotes	Laird
T1139	4441-4443	,	denotes	,
T1140	4443-4445	NNP	denotes	H.
T1141	4446-4449	NNP	denotes	Cai
T1142	4449-4451	,	denotes	,
T1143	4451-4453	NNP	denotes	P.
T1144	4454-4456	NNP	denotes	C.
T1145	4457-4461	NNP	denotes	Wong
T1146	4461-4463	,	denotes	,
T1147	4463-4471	JJ	denotes	personal
T1148	4472-4485	NN	denotes	communication
T1149	4485-4486	-RRB-	denotes	)
T1150	4486-4488	,	denotes	,
T1151	4488-4490	PRP	denotes	it
T1152	4491-4493	VBZ	denotes	is
T1153	4494-4497	RB	denotes	not
T1154	4504-4511	IN	denotes	whether
T1155	4526-4531	VB	denotes	clear
T1156	4512-4515	DT	denotes	the
T1157	4516-4521	NN	denotes	brain
T1158	4522-4525	MD	denotes	can
T1159	4532-4544	JJ	denotes	pre-existing
T1160	4553-4561	NNS	denotes	deposits
T1161	4545-4552	NN	denotes	amyloid
T1162	4562-4566	IN	denotes	once
T1163	4593-4603	VBN	denotes	suppressed
T1164	4567-4577	NN	denotes	production
T1165	4578-4580	IN	denotes	of
T1166	4581-4583	NN	denotes	Aβ
T1167	4584-4587	VBZ	denotes	has
T1168	4588-4592	VBN	denotes	been
T1169	4603-4604	.	denotes	.
T1170	4604-4736	sentence	denotes	Clearly, the amyloid-lowering approach should be rigorously examined in animal models before these reagents are tested in patients.
T1171	4605-4612	RB	denotes	Clearly
T1172	4665-4673	VBN	denotes	examined
T1173	4612-4614	,	denotes	,
T1174	4614-4617	DT	denotes	the
T1175	4635-4643	NN	denotes	approach
T1176	4618-4625	NN	denotes	amyloid
T1177	4626-4634	VBG	denotes	lowering
T1178	4625-4626	HYPH	denotes	-
T1179	4644-4650	MD	denotes	should
T1180	4651-4653	VB	denotes	be
T1181	4654-4664	RB	denotes	rigorously
T1182	4674-4676	IN	denotes	in
T1183	4677-4683	NN	denotes	animal
T1184	4684-4690	NNS	denotes	models
T1185	4691-4697	IN	denotes	before
T1186	4717-4723	VBN	denotes	tested
T1187	4698-4703	DT	denotes	these
T1188	4704-4712	NNS	denotes	reagents
T1189	4713-4716	VBP	denotes	are
T1190	4724-4726	IN	denotes	in
T1191	4727-4735	NNS	denotes	patients
T1192	4735-4736	.	denotes	.
T1193	4736-4839	sentence	denotes	However, the chemical secretase inhibitors most likely to reach human trials are still in development.
T1194	4737-4744	RB	denotes	However
T1195	4814-4817	VBP	denotes	are
T1196	4744-4746	,	denotes	,
T1197	4746-4749	DT	denotes	the
T1198	4769-4779	NNS	denotes	inhibitors
T1199	4750-4758	JJ	denotes	chemical
T1200	4759-4768	NN	denotes	secretase
T1201	4780-4784	RBS	denotes	most
T1202	4785-4791	JJ	denotes	likely
T1203	4792-4794	TO	denotes	to
T1204	4795-4800	VB	denotes	reach
T1205	4801-4806	JJ	denotes	human
T1206	4807-4813	NNS	denotes	trials
T1207	4818-4823	RB	denotes	still
T1208	4824-4826	IN	denotes	in
T1209	4827-4838	NN	denotes	development
T1210	4838-4839	.	denotes	.
T1211	4839-4948	sentence	denotes	Therefore, we developed a mouse model of Alzheimer-type amyloid that expresses a controllable APP transgene.
T1212	4840-4849	RB	denotes	Therefore
T1213	4854-4863	VBD	denotes	developed
T1214	4849-4851	,	denotes	,
T1215	4851-4853	PRP	denotes	we
T1216	4864-4865	DT	denotes	a
T1217	4872-4877	NN	denotes	model
T1218	4866-4871	NN	denotes	mouse
T1219	4878-4880	IN	denotes	of
T1220	4881-4890	NNP	denotes	Alzheimer
T1221	4891-4895	NN	denotes	type
T1222	4890-4891	HYPH	denotes	-
T1223	4896-4903	NN	denotes	amyloid
T1224	4904-4908	WDT	denotes	that
T1225	4909-4918	VBZ	denotes	expresses
T1226	4919-4920	DT	denotes	a
T1227	4938-4947	NN	denotes	transgene
T1228	4921-4933	JJ	denotes	controllable
T1229	4934-4937	NN	denotes	APP
T1230	4947-4948	.	denotes	.
T1231	4948-5082	sentence	denotes	This system, commonly known as the tet-off system, can be regulated by analogs of tetracycline administered in food or water [12,13].
T1232	4949-4953	DT	denotes	This
T1233	4954-4960	NN	denotes	system
T1234	5007-5016	VBN	denotes	regulated
T1235	4960-4962	,	denotes	,
T1236	4962-4970	RB	denotes	commonly
T1237	4971-4976	VBN	denotes	known
T1238	4977-4979	IN	denotes	as
T1239	4980-4983	DT	denotes	the
T1240	4992-4998	NN	denotes	system
T1241	4984-4987	NN	denotes	tet
T1242	4988-4991	NN	denotes	off
T1243	4987-4988	HYPH	denotes	-
T1244	4998-5000	,	denotes	,
T1245	5000-5003	MD	denotes	can
T1246	5004-5006	VB	denotes	be
T1247	5017-5019	IN	denotes	by
T1248	5020-5027	NNS	denotes	analogs
T1249	5028-5030	IN	denotes	of
T1250	5031-5043	NN	denotes	tetracycline
T1251	5044-5056	VBN	denotes	administered
T1252	5057-5059	IN	denotes	in
T1253	5060-5064	NN	denotes	food
T1254	5065-5067	CC	denotes	or
T1255	5068-5073	NN	denotes	water
T1256	5074-5075	-LRB-	denotes	[
T1257	5078-5080	CD	denotes	13
T1258	5075-5077	CD	denotes	12
T1259	5077-5078	,	denotes	,
T1260	5080-5081	-RRB-	denotes	]
T1261	5081-5082	.	denotes	.
T1262	5082-5482	sentence	denotes	The strong expression levels produced with the tet-off vectors, combined with the ability to reduce this expression by several orders of magnitude with tetracycline [14], allowed for a stringent test of how a highly effective pharmaceutical inhibitor of Aβ production would impact the progression of amyloid pathology and whether reversal of these lesions might be possible following such treatment.
T1263	5083-5086	DT	denotes	The
T1264	5105-5111	NNS	denotes	levels
T1265	5087-5093	JJ	denotes	strong
T1266	5094-5104	NN	denotes	expression
T1267	5254-5261	VBD	denotes	allowed
T1268	5112-5120	VBN	denotes	produced
T1269	5121-5125	IN	denotes	with
T1270	5126-5129	DT	denotes	the
T1271	5138-5145	NNS	denotes	vectors
T1272	5130-5133	NN	denotes	tet
T1273	5134-5137	NN	denotes	off
T1274	5133-5134	HYPH	denotes	-
T1275	5145-5147	,	denotes	,
T1276	5147-5155	VBN	denotes	combined
T1277	5156-5160	IN	denotes	with
T1278	5161-5164	DT	denotes	the
T1279	5165-5172	NN	denotes	ability
T1280	5173-5175	TO	denotes	to
T1281	5176-5182	VB	denotes	reduce
T1282	5183-5187	DT	denotes	this
T1283	5188-5198	NN	denotes	expression
T1284	5199-5201	IN	denotes	by
T1285	5202-5209	JJ	denotes	several
T1286	5210-5216	NNS	denotes	orders
T1287	5217-5219	IN	denotes	of
T1288	5220-5229	NN	denotes	magnitude
T1289	5230-5234	IN	denotes	with
T1290	5235-5247	NN	denotes	tetracycline
T1291	5248-5249	-LRB-	denotes	[
T1292	5249-5251	CD	denotes	14
T1293	5251-5252	-RRB-	denotes	]
T1294	5252-5254	,	denotes	,
T1295	5262-5265	IN	denotes	for
T1296	5266-5267	DT	denotes	a
T1297	5278-5282	NN	denotes	test
T1298	5268-5277	JJ	denotes	stringent
T1299	5283-5285	IN	denotes	of
T1300	5286-5289	WRB	denotes	how
T1301	5357-5363	VB	denotes	impact
T1302	5290-5291	DT	denotes	a
T1303	5324-5333	NN	denotes	inhibitor
T1304	5292-5298	RB	denotes	highly
T1305	5299-5308	JJ	denotes	effective
T1306	5309-5323	JJ	denotes	pharmaceutical
T1307	5334-5336	IN	denotes	of
T1308	5337-5339	NN	denotes	Aβ
T1309	5340-5350	NN	denotes	production
T1310	5351-5356	MD	denotes	would
T1311	5364-5367	DT	denotes	the
T1312	5368-5379	NN	denotes	progression
T1313	5380-5382	IN	denotes	of
T1314	5383-5390	NN	denotes	amyloid
T1315	5391-5400	NN	denotes	pathology
T1316	5401-5404	CC	denotes	and
T1317	5405-5412	IN	denotes	whether
T1318	5445-5447	VB	denotes	be
T1319	5413-5421	NN	denotes	reversal
T1320	5422-5424	IN	denotes	of
T1321	5425-5430	DT	denotes	these
T1322	5431-5438	NNS	denotes	lesions
T1323	5439-5444	MD	denotes	might
T1324	5448-5456	JJ	denotes	possible
T1325	5457-5466	VBG	denotes	following
T1326	5467-5471	JJ	denotes	such
T1327	5472-5481	NN	denotes	treatment
T1328	5481-5482	.	denotes	.
T1535	5493-5502	NN	denotes	Transgene
T1536	5503-5515	NN	denotes	Construction
T1537	5515-5915	sentence	denotes	We created a tetracycline-responsive chimeric mouse/human APP695Swedish/Indiana (swe/ind) vector by replacing the promoter region of the moPrP.XhoI vector (also known as pPrPpE1/E2,3sal [15]) with the tetracycline-responsive promoter of pTetSplice (Life Technologies, Rockville, Maryland, United States), and then ligating mouse APP with a humanized Aβ domain (mo/huAPP695) cDNA into the new vector.
T1538	5516-5518	PRP	denotes	We
T1539	5519-5526	VBD	denotes	created
T1540	5527-5528	DT	denotes	a
T1541	5606-5612	NN	denotes	vector
T1542	5529-5541	NN	denotes	tetracycline
T1543	5542-5552	JJ	denotes	responsive
T1544	5541-5542	HYPH	denotes	-
T1545	5553-5561	JJ	denotes	chimeric
T1546	5562-5567	NN	denotes	mouse
T1547	5567-5568	HYPH	denotes	/
T1548	5568-5573	JJ	denotes	human
T1549	5574-5587	NN	denotes	APP695Swedish
T1550	5588-5595	NNP	denotes	Indiana
T1551	5587-5588	HYPH	denotes	/
T1552	5596-5597	-LRB-	denotes	(
T1553	5597-5600	NN	denotes	swe
T1554	5601-5604	NN	denotes	ind
T1555	5600-5601	HYPH	denotes	/
T1556	5604-5605	-RRB-	denotes	)
T1557	5613-5615	IN	denotes	by
T1558	5616-5625	VBG	denotes	replacing
T1559	5626-5629	DT	denotes	the
T1560	5639-5645	NN	denotes	region
T1561	5630-5638	NN	denotes	promoter
T1562	5646-5648	IN	denotes	of
T1563	5649-5652	DT	denotes	the
T1564	5664-5670	NN	denotes	vector
T1565	5653-5663	NN	denotes	moPrP.XhoI
T1566	5671-5672	-LRB-	denotes	(
T1567	5677-5682	VBN	denotes	known
T1568	5672-5676	RB	denotes	also
T1569	5683-5685	IN	denotes	as
T1570	5686-5693	NN	denotes	pPrPpE1
T1571	5697-5701	NN	denotes	3sal
T1572	5693-5694	HYPH	denotes	/
T1573	5694-5696	NN	denotes	E2
T1574	5696-5697	,	denotes	,
T1575	5702-5703	-LRB-	denotes	[
T1576	5703-5705	CD	denotes	15
T1577	5705-5706	-RRB-	denotes	]
T1578	5706-5707	-RRB-	denotes	)
T1579	5708-5712	IN	denotes	with
T1580	5713-5716	DT	denotes	the
T1581	5741-5749	NN	denotes	promoter
T1582	5717-5729	NN	denotes	tetracycline
T1583	5730-5740	JJ	denotes	responsive
T1584	5729-5730	HYPH	denotes	-
T1585	5750-5752	IN	denotes	of
T1586	5753-5763	NN	denotes	pTetSplice
T1587	5764-5765	-LRB-	denotes	(
T1588	5770-5782	NNP	denotes	Technologies
T1589	5765-5769	NNP	denotes	Life
T1590	5782-5784	,	denotes	,
T1591	5784-5793	NNP	denotes	Rockville
T1592	5793-5795	,	denotes	,
T1593	5795-5803	NNP	denotes	Maryland
T1594	5803-5805	,	denotes	,
T1595	5805-5811	NNP	denotes	United
T1596	5812-5818	NNP	denotes	States
T1597	5818-5819	-RRB-	denotes	)
T1598	5819-5821	,	denotes	,
T1599	5821-5824	CC	denotes	and
T1600	5825-5829	RB	denotes	then
T1601	5830-5838	VBG	denotes	ligating
T1602	5839-5844	NN	denotes	mouse
T1603	5845-5848	NN	denotes	APP
T1604	5849-5853	IN	denotes	with
T1605	5854-5855	DT	denotes	a
T1606	5869-5875	NN	denotes	domain
T1607	5856-5865	JJ	denotes	humanized
T1608	5866-5868	NN	denotes	Aβ
T1609	5890-5894	NN	denotes	cDNA
T1610	5876-5877	-LRB-	denotes	(
T1611	5877-5879	NN	denotes	mo
T1612	5880-5888	NN	denotes	huAPP695
T1613	5879-5880	HYPH	denotes	/
T1614	5888-5889	-RRB-	denotes	)
T1615	5895-5899	IN	denotes	into
T1616	5900-5903	DT	denotes	the
T1617	5908-5914	NN	denotes	vector
T1618	5904-5907	JJ	denotes	new
T1619	5914-5915	.	denotes	.
T1620	5915-6285	sentence	denotes	We began by cloning the tetracycline-responsive promoter (bp 6–481) from pTetSplice by PCR using primers that added external BamHI and NotI sites to the 5′ end and a BamHI site to the 3′ end, while destroying XhoI and BamHI sites within the promoter (forward: GCC GGA TCC GCG GCC GCC GTC GAG TTT ACC ACT CCC TAT C; reverse: GCC GGA TCC ACT CTA GAA GAT CCC CGG GTA CCG).
T1621	5916-5918	PRP	denotes	We
T1622	5919-5924	VBD	denotes	began
T1623	5925-5927	IN	denotes	by
T1624	5928-5935	VBG	denotes	cloning
T1625	5936-5939	DT	denotes	the
T1626	5964-5972	NN	denotes	promoter
T1627	5940-5952	NN	denotes	tetracycline
T1628	5953-5963	JJ	denotes	responsive
T1629	5952-5953	HYPH	denotes	-
T1630	5973-5974	-LRB-	denotes	(
T1631	5977-5978	CD	denotes	6
T1632	5974-5976	NN	denotes	bp
T1633	5978-5979	SYM	denotes	–
T1634	5979-5982	CD	denotes	481
T1635	5982-5983	-RRB-	denotes	)
T1636	5984-5988	IN	denotes	from
T1637	5989-5999	NN	denotes	pTetSplice
T1638	6000-6002	IN	denotes	by
T1639	6003-6006	NN	denotes	PCR
T1640	6007-6012	VBG	denotes	using
T1641	6013-6020	NNS	denotes	primers
T1642	6021-6025	WDT	denotes	that
T1643	6026-6031	VBD	denotes	added
T1644	6032-6040	JJ	denotes	external
T1645	6056-6061	NNS	denotes	sites
T1646	6041-6046	NN	denotes	BamHI
T1647	6047-6050	CC	denotes	and
T1648	6051-6055	NN	denotes	NotI
T1649	6062-6064	IN	denotes	to
T1650	6065-6068	DT	denotes	the
T1651	6072-6075	NN	denotes	end
T1652	6069-6070	CD	denotes	5
T1653	6070-6071	SYM	denotes	′
T1654	6076-6079	CC	denotes	and
T1655	6080-6081	DT	denotes	a
T1656	6088-6092	NN	denotes	site
T1657	6082-6087	NN	denotes	BamHI
T1658	6093-6095	IN	denotes	to
T1659	6096-6099	DT	denotes	the
T1660	6103-6106	NN	denotes	end
T1661	6100-6101	CD	denotes	3
T1662	6101-6102	SYM	denotes	′
T1663	6106-6108	,	denotes	,
T1664	6108-6113	IN	denotes	while
T1665	6114-6124	VBG	denotes	destroying
T1666	6125-6129	NN	denotes	XhoI
T1667	6140-6145	NNS	denotes	sites
T1668	6130-6133	CC	denotes	and
T1669	6134-6139	NN	denotes	BamHI
T1670	6146-6152	IN	denotes	within
T1671	6153-6156	DT	denotes	the
T1672	6157-6165	NN	denotes	promoter
T1673	6166-6167	-LRB-	denotes	(
T1674	6280-6283	NN	denotes	CCG
T1675	6167-6174	NN	denotes	forward
T1676	6228-6229	NN	denotes	C
T1677	6174-6176	:	denotes	:
T1678	6176-6179	NN	denotes	GCC
T1679	6180-6183	NN	denotes	GGA
T1680	6184-6187	NN	denotes	TCC
T1681	6188-6191	NN	denotes	GCG
T1682	6192-6195	NN	denotes	GCC
T1683	6196-6199	NN	denotes	GCC
T1684	6200-6203	NN	denotes	GTC
T1685	6204-6207	NN	denotes	GAG
T1686	6208-6211	NN	denotes	TTT
T1687	6212-6215	NN	denotes	ACC
T1688	6216-6219	NN	denotes	ACT
T1689	6220-6223	NN	denotes	CCC
T1690	6224-6227	NN	denotes	TAT
T1691	6229-6230	:	denotes	;
T1692	6231-6238	NN	denotes	reverse
T1693	6238-6240	:	denotes	:
T1694	6240-6243	NN	denotes	GCC
T1695	6244-6247	NN	denotes	GGA
T1696	6248-6251	NN	denotes	TCC
T1697	6252-6255	NN	denotes	ACT
T1698	6256-6259	NN	denotes	CTA
T1699	6260-6263	NN	denotes	GAA
T1700	6264-6267	NN	denotes	GAT
T1701	6268-6271	NN	denotes	CCC
T1702	6272-6275	NN	denotes	CGG
T1703	6276-6279	NN	denotes	GTA
T1704	6283-6284	-RRB-	denotes	)
T1705	6284-6285	.	denotes	.
T1706	6285-6551	sentence	denotes	We then isolated the moPrP.XhoI intron by amplification with primers that added an external BamHI site to the 5′ end of exon 1 and ran through the Asp718 site of exon 2 (forward: GCC GGA TCC GAT CAG CAG ACC GAT TCT GG; reverse: GCC GGT ACC ACT AGG AAG GCA GAA TGC).
T1707	6286-6288	PRP	denotes	We
T1708	6294-6302	VBD	denotes	isolated
T1709	6289-6293	RB	denotes	then
T1710	6303-6306	DT	denotes	the
T1711	6318-6324	NN	denotes	intron
T1712	6307-6317	NN	denotes	moPrP.XhoI
T1713	6325-6327	IN	denotes	by
T1714	6328-6341	NN	denotes	amplification
T1715	6342-6346	IN	denotes	with
T1716	6347-6354	NNS	denotes	primers
T1717	6355-6359	WDT	denotes	that
T1718	6360-6365	VBD	denotes	added
T1719	6366-6368	DT	denotes	an
T1720	6384-6388	NN	denotes	site
T1721	6369-6377	JJ	denotes	external
T1722	6378-6383	NN	denotes	BamHI
T1723	6389-6391	IN	denotes	to
T1724	6392-6395	DT	denotes	the
T1725	6399-6402	NN	denotes	end
T1726	6396-6397	CD	denotes	5
T1727	6397-6398	SYM	denotes	′
T1728	6403-6405	IN	denotes	of
T1729	6406-6410	NN	denotes	exon
T1730	6411-6412	CD	denotes	1
T1731	6413-6416	CC	denotes	and
T1732	6417-6420	VBD	denotes	ran
T1733	6421-6428	IN	denotes	through
T1734	6429-6432	DT	denotes	the
T1735	6440-6444	NN	denotes	site
T1736	6433-6439	NN	denotes	Asp718
T1737	6445-6447	IN	denotes	of
T1738	6448-6452	NN	denotes	exon
T1739	6453-6454	CD	denotes	2
T1740	6455-6456	-LRB-	denotes	(
T1741	6546-6549	NN	denotes	TGC
T1742	6456-6463	NN	denotes	forward
T1743	6501-6503	NN	denotes	GG
T1744	6463-6465	:	denotes	:
T1745	6465-6468	NN	denotes	GCC
T1746	6469-6472	NN	denotes	GGA
T1747	6473-6476	NN	denotes	TCC
T1748	6477-6480	NN	denotes	GAT
T1749	6481-6484	NN	denotes	CAG
T1750	6485-6488	NN	denotes	CAG
T1751	6489-6492	NN	denotes	ACC
T1752	6493-6496	NN	denotes	GAT
T1753	6497-6500	NN	denotes	TCT
T1754	6503-6504	:	denotes	;
T1755	6505-6512	NN	denotes	reverse
T1756	6512-6514	:	denotes	:
T1757	6514-6517	NN	denotes	GCC
T1758	6518-6521	NN	denotes	GGT
T1759	6522-6525	NN	denotes	ACC
T1760	6526-6529	NN	denotes	ACT
T1761	6530-6533	NN	denotes	AGG
T1762	6534-6537	NN	denotes	AAG
T1763	6538-6541	NN	denotes	GCA
T1764	6542-6545	NN	denotes	GAA
T1765	6549-6550	-RRB-	denotes	)
T1766	6550-6551	.	denotes	.
T1767	6551-6902	sentence	denotes	This 2-kb intron fragment was cloned into a TA cloning vector (Invitrogen, Carlsbad, California, United States), then excised by Asp718 digestion and ligated to the 6.8-kb Asp718 fragment of moPrP.XhoI containing exon 2, exon 3, the 3′ UTR, and pBluescript to generate an intermediate vector with all three exons and a central intron but no promoter.
T1768	6552-6556	DT	denotes	This
T1769	6569-6577	NN	denotes	fragment
T1770	6557-6558	CD	denotes	2
T1771	6559-6561	NN	denotes	kb
T1772	6558-6559	HYPH	denotes	-
T1773	6562-6568	NN	denotes	intron
T1774	6582-6588	VBN	denotes	cloned
T1775	6578-6581	VBD	denotes	was
T1776	6589-6593	IN	denotes	into
T1777	6594-6595	DT	denotes	a
T1778	6607-6613	NN	denotes	vector
T1779	6596-6598	NN	denotes	TA
T1780	6599-6606	NN	denotes	cloning
T1781	6614-6615	-LRB-	denotes	(
T1782	6615-6625	NNP	denotes	Invitrogen
T1783	6625-6627	,	denotes	,
T1784	6627-6635	NNP	denotes	Carlsbad
T1785	6635-6637	,	denotes	,
T1786	6637-6647	NNP	denotes	California
T1787	6647-6649	,	denotes	,
T1788	6649-6655	NNP	denotes	United
T1789	6656-6662	NNP	denotes	States
T1790	6662-6663	-RRB-	denotes	)
T1791	6663-6665	,	denotes	,
T1792	6665-6669	RB	denotes	then
T1793	6670-6677	VBN	denotes	excised
T1794	6678-6680	IN	denotes	by
T1795	6681-6687	NN	denotes	Asp718
T1796	6688-6697	NN	denotes	digestion
T1797	6698-6701	CC	denotes	and
T1798	6702-6709	VBN	denotes	ligated
T1799	6710-6712	IN	denotes	to
T1800	6713-6716	DT	denotes	the
T1801	6731-6739	NN	denotes	fragment
T1802	6717-6720	CD	denotes	6.8
T1803	6721-6723	NN	denotes	kb
T1804	6720-6721	HYPH	denotes	-
T1805	6724-6730	NN	denotes	Asp718
T1806	6740-6742	IN	denotes	of
T1807	6743-6753	NN	denotes	moPrP.XhoI
T1808	6754-6764	VBG	denotes	containing
T1809	6765-6769	NN	denotes	exon
T1810	6770-6771	CD	denotes	2
T1811	6771-6773	,	denotes	,
T1812	6773-6777	NN	denotes	exon
T1813	6778-6779	CD	denotes	3
T1814	6779-6781	,	denotes	,
T1815	6781-6784	DT	denotes	the
T1816	6788-6791	NN	denotes	UTR
T1817	6785-6786	CD	denotes	3
T1818	6786-6787	SYM	denotes	′
T1819	6791-6793	,	denotes	,
T1820	6793-6796	CC	denotes	and
T1821	6797-6808	NN	denotes	pBluescript
T1822	6809-6811	TO	denotes	to
T1823	6812-6820	VB	denotes	generate
T1824	6821-6823	DT	denotes	an
T1825	6837-6843	NN	denotes	vector
T1826	6824-6836	JJ	denotes	intermediate
T1827	6844-6848	IN	denotes	with
T1828	6849-6852	DT	denotes	all
T1829	6859-6864	NNS	denotes	exons
T1830	6853-6858	CD	denotes	three
T1831	6865-6868	CC	denotes	and
T1832	6869-6870	DT	denotes	a
T1833	6879-6885	NN	denotes	intron
T1834	6871-6878	JJ	denotes	central
T1835	6886-6889	CC	denotes	but
T1836	6890-6892	DT	denotes	no
T1837	6893-6901	NN	denotes	promoter
T1838	6901-6902	.	denotes	.
T1839	6902-7057	sentence	denotes	This vector was then opened at the BamHI site introduced by the intron cloning primer, and ligated to the 0.5-kb BamHI-cut tetracycline promoter fragment.
T1840	6903-6907	DT	denotes	This
T1841	6908-6914	NN	denotes	vector
T1842	6924-6930	VBN	denotes	opened
T1843	6915-6918	VBD	denotes	was
T1844	6919-6923	RB	denotes	then
T1845	6931-6933	IN	denotes	at
T1846	6934-6937	DT	denotes	the
T1847	6944-6948	NN	denotes	site
T1848	6938-6943	NN	denotes	BamHI
T1849	6949-6959	VBN	denotes	introduced
T1850	6960-6962	IN	denotes	by
T1851	6963-6966	DT	denotes	the
T1852	6982-6988	NN	denotes	primer
T1853	6967-6973	NN	denotes	intron
T1854	6974-6981	NN	denotes	cloning
T1855	6988-6990	,	denotes	,
T1856	6990-6993	CC	denotes	and
T1857	6994-7001	VBN	denotes	ligated
T1858	7002-7004	IN	denotes	to
T1859	7005-7008	DT	denotes	the
T1860	7048-7056	NN	denotes	fragment
T1861	7009-7012	CD	denotes	0.5
T1862	7013-7015	NN	denotes	kb
T1863	7012-7013	HYPH	denotes	-
T1864	7016-7021	NN	denotes	BamHI
T1865	7022-7025	NN	denotes	cut
T1866	7021-7022	HYPH	denotes	-
T1867	7026-7038	NN	denotes	tetracycline
T1868	7039-7047	NN	denotes	promoter
T1869	7056-7057	.	denotes	.
T1870	7057-7273	sentence	denotes	This ligation generated a 9.3-kb vector encoding the tetracycline promoter from pTetSplice with two exons, one intron, and the original 3′ UTR of the moPrP.XhoI vector, all carried in the pBluescript cloning vector.
T1871	7058-7062	DT	denotes	This
T1872	7063-7071	NN	denotes	ligation
T1873	7072-7081	VBD	denotes	generated
T1874	7082-7083	DT	denotes	a
T1875	7091-7097	NN	denotes	vector
T1876	7084-7087	CD	denotes	9.3
T1877	7088-7090	NN	denotes	kb
T1878	7087-7088	HYPH	denotes	-
T1879	7098-7106	VBG	denotes	encoding
T1880	7107-7110	DT	denotes	the
T1881	7124-7132	NN	denotes	promoter
T1882	7111-7123	NN	denotes	tetracycline
T1883	7133-7137	IN	denotes	from
T1884	7138-7148	NN	denotes	pTetSplice
T1885	7149-7153	IN	denotes	with
T1886	7154-7157	CD	denotes	two
T1887	7158-7163	NNS	denotes	exons
T1888	7163-7165	,	denotes	,
T1889	7165-7168	CD	denotes	one
T1890	7169-7175	NN	denotes	intron
T1891	7175-7177	,	denotes	,
T1892	7177-7180	CC	denotes	and
T1893	7181-7184	DT	denotes	the
T1894	7197-7200	NN	denotes	UTR
T1895	7185-7193	JJ	denotes	original
T1896	7194-7195	CD	denotes	3
T1897	7195-7196	SYM	denotes	′
T1898	7201-7203	IN	denotes	of
T1899	7204-7207	DT	denotes	the
T1900	7219-7225	NN	denotes	vector
T1901	7208-7218	NN	denotes	moPrP.XhoI
T1902	7225-7227	,	denotes	,
T1903	7227-7230	RB	denotes	all
T1904	7231-7238	VBN	denotes	carried
T1905	7239-7241	IN	denotes	in
T1906	7242-7245	DT	denotes	the
T1907	7266-7272	NN	denotes	vector
T1908	7246-7257	NNP	denotes	pBluescript
T1909	7258-7265	NN	denotes	cloning
T1910	7272-7273	.	denotes	.
T1911	7273-7723	sentence	denotes	We incorporated the Swedish (KM570/571NL) and Indiana (V617F) mutations into the mo/huAPP695 cDNA (in BS-KS) by PCR using a four-primer strategy: first, two partially overlapping products were generated in separate reactions using primers that encode the desired mutations (Swedish forward: GGA GAT CTC TGA AGT GAA TCT GGA TGC AGA ATT CCG/Indiana reverse: GGG TGA TGA AAA TCA CGG TTG C; Indiana forward: CAA CCG TGA TTT TCA TCA CCC TGG/M13 reverse).
T1912	7274-7276	PRP	denotes	We
T1913	7277-7289	VBD	denotes	incorporated
T1914	7467-7476	VBN	denotes	generated
T1915	7290-7293	DT	denotes	the
T1916	7336-7345	NNS	denotes	mutations
T1917	7294-7301	NNP	denotes	Swedish
T1918	7302-7303	-LRB-	denotes	(
T1919	7309-7314	NN	denotes	571NL
T1920	7303-7308	NN	denotes	KM570
T1921	7308-7309	HYPH	denotes	/
T1922	7314-7315	-RRB-	denotes	)
T1923	7316-7319	CC	denotes	and
T1924	7320-7327	NNP	denotes	Indiana
T1925	7328-7329	-LRB-	denotes	(
T1926	7329-7334	NN	denotes	V617F
T1927	7334-7335	-RRB-	denotes	)
T1928	7346-7350	IN	denotes	into
T1929	7351-7354	DT	denotes	the
T1930	7367-7371	NN	denotes	cDNA
T1931	7355-7357	NN	denotes	mo
T1932	7358-7366	NN	denotes	huAPP695
T1933	7357-7358	HYPH	denotes	/
T1934	7372-7373	-LRB-	denotes	(
T1935	7373-7375	IN	denotes	in
T1936	7376-7378	NN	denotes	BS
T1937	7379-7381	NN	denotes	KS
T1938	7378-7379	HYPH	denotes	-
T1939	7381-7382	-RRB-	denotes	)
T1940	7383-7385	IN	denotes	by
T1941	7386-7389	NN	denotes	PCR
T1942	7390-7395	VBG	denotes	using
T1943	7396-7397	DT	denotes	a
T1944	7410-7418	NN	denotes	strategy
T1945	7398-7402	CD	denotes	four
T1946	7403-7409	NN	denotes	primer
T1947	7402-7403	HYPH	denotes	-
T1948	7418-7420	:	denotes	:
T1949	7420-7425	RB	denotes	first
T1950	7425-7427	,	denotes	,
T1951	7427-7430	CD	denotes	two
T1952	7453-7461	NNS	denotes	products
T1953	7431-7440	RB	denotes	partially
T1954	7441-7452	VBG	denotes	overlapping
T1955	7462-7466	VBD	denotes	were
T1956	7477-7479	IN	denotes	in
T1957	7480-7488	JJ	denotes	separate
T1958	7489-7498	NNS	denotes	reactions
T1959	7499-7504	VBG	denotes	using
T1960	7505-7512	NNS	denotes	primers
T1961	7513-7517	WDT	denotes	that
T1962	7518-7524	VBP	denotes	encode
T1963	7525-7528	DT	denotes	the
T1964	7537-7546	NNS	denotes	mutations
T1965	7529-7536	VBN	denotes	desired
T1966	7547-7548	-LRB-	denotes	(
T1967	7710-7713	NN	denotes	M13
T1968	7548-7555	NNP	denotes	Swedish
T1969	7556-7563	NN	denotes	forward
T1970	7609-7612	NN	denotes	CCG
T1971	7563-7565	:	denotes	:
T1972	7565-7568	NN	denotes	GGA
T1973	7569-7572	NN	denotes	GAT
T1974	7573-7576	NN	denotes	CTC
T1975	7577-7580	NN	denotes	TGA
T1976	7581-7584	NN	denotes	AGT
T1977	7585-7588	NN	denotes	GAA
T1978	7589-7592	NN	denotes	TCT
T1979	7593-7596	NNP	denotes	GGA
T1980	7597-7600	NNP	denotes	TGC
T1981	7601-7604	NNP	denotes	AGA
T1982	7605-7608	NNP	denotes	ATT
T1983	7658-7659	NN	denotes	C
T1984	7612-7613	HYPH	denotes	/
T1985	7613-7620	NNP	denotes	Indiana
T1986	7621-7628	NN	denotes	reverse
T1987	7628-7630	:	denotes	:
T1988	7630-7633	NN	denotes	GGG
T1989	7634-7637	NN	denotes	TGA
T1990	7638-7641	NN	denotes	TGA
T1991	7642-7645	NN	denotes	AAA
T1992	7646-7649	NN	denotes	TCA
T1993	7650-7653	NN	denotes	CGG
T1994	7654-7657	NN	denotes	TTG
T1995	7659-7660	:	denotes	;
T1996	7661-7668	NNP	denotes	Indiana
T1997	7669-7676	NN	denotes	forward
T1998	7676-7678	:	denotes	:
T1999	7678-7681	NN	denotes	CAA
T2000	7682-7685	NN	denotes	CCG
T2001	7686-7689	NN	denotes	TGA
T2002	7690-7693	NN	denotes	TTT
T2003	7694-7697	NN	denotes	TCA
T2004	7698-7701	NN	denotes	TCA
T2005	7702-7705	NN	denotes	CCC
T2006	7706-7709	NN	denotes	TGG
T2007	7709-7710	HYPH	denotes	/
T2008	7714-7721	RB	denotes	reverse
T2009	7721-7722	-RRB-	denotes	)
T2010	7722-7723	.	denotes	.
T2011	7723-7847	sentence	denotes	The two PCR products were ligated, digested with BglII and ApaI and cloned back into the original mo/huAPP695-BS-KS vector.
T2012	7724-7727	DT	denotes	The
T2013	7736-7744	NNS	denotes	products
T2014	7728-7731	CD	denotes	two
T2015	7732-7735	NN	denotes	PCR
T2016	7750-7757	VBN	denotes	ligated
T2017	7745-7749	VBD	denotes	were
T2018	7757-7759	,	denotes	,
T2019	7759-7767	VBN	denotes	digested
T2020	7768-7772	IN	denotes	with
T2021	7773-7778	NN	denotes	BglII
T2022	7779-7782	CC	denotes	and
T2023	7783-7787	NN	denotes	ApaI
T2024	7788-7791	CC	denotes	and
T2025	7792-7798	VBN	denotes	cloned
T2026	7799-7803	RB	denotes	back
T2027	7804-7808	IN	denotes	into
T2028	7809-7812	DT	denotes	the
T2029	7840-7846	NN	denotes	vector
T2030	7813-7821	JJ	denotes	original
T2031	7822-7824	NN	denotes	mo
T2032	7837-7839	NN	denotes	KS
T2033	7824-7825	HYPH	denotes	/
T2034	7825-7833	NN	denotes	huAPP695
T2035	7833-7834	HYPH	denotes	-
T2036	7834-7836	NN	denotes	BS
T2037	7836-7837	HYPH	denotes	-
T2038	7846-7847	.	denotes	.
T2039	7847-7974	sentence	denotes	Finally, the new APP695swe/ind was subcloned into the XhoI site of the moPrP-tetP vector from above to complete the construct.
T2040	7848-7855	RB	denotes	Finally
T2041	7883-7892	VBN	denotes	subcloned
T2042	7855-7857	,	denotes	,
T2043	7857-7860	DT	denotes	the
T2044	7875-7878	NN	denotes	ind
T2045	7861-7864	JJ	denotes	new
T2046	7865-7874	NN	denotes	APP695swe
T2047	7874-7875	HYPH	denotes	/
T2048	7879-7882	VBD	denotes	was
T2049	7893-7897	IN	denotes	into
T2050	7898-7901	DT	denotes	the
T2051	7907-7911	NN	denotes	site
T2052	7902-7906	NN	denotes	XhoI
T2053	7912-7914	IN	denotes	of
T2054	7915-7918	DT	denotes	the
T2055	7930-7936	NN	denotes	vector
T2056	7919-7924	NN	denotes	moPrP
T2057	7925-7929	NN	denotes	tetP
T2058	7924-7925	HYPH	denotes	-
T2059	7937-7941	IN	denotes	from
T2060	7942-7947	RB	denotes	above
T2061	7948-7950	TO	denotes	to
T2062	7951-7959	VB	denotes	complete
T2063	7960-7963	DT	denotes	the
T2064	7964-7973	NN	denotes	construct
T2065	7973-7974	.	denotes	.
T2158	7976-7986	JJ	denotes	Pronuclear
T2159	7987-7996	NN	denotes	Injection
T2160	7996-7998	,	denotes	,
T2161	7998-8007	NN	denotes	Screening
T2162	8008-8010	IN	denotes	of
T2163	8011-8019	NNS	denotes	Founders
T2164	8019-8021	,	denotes	,
T2165	8021-8024	CC	denotes	and
T2166	8025-8036	NN	denotes	Maintenance
T2167	8037-8039	IN	denotes	of
T2168	8040-8043	DT	denotes	the
T2169	8044-8049	NNS	denotes	Lines
T2170	8049-8164	sentence	denotes	The moPrP-tetP-mo/huAPP695swe/ind vector was linearized and the pBluescript domain excised by digestion with NotI.
T2171	8050-8053	DT	denotes	The
T2172	8084-8090	NN	denotes	vector
T2173	8054-8059	NN	denotes	moPrP
T2174	8080-8083	NN	denotes	ind
T2175	8059-8060	HYPH	denotes	-
T2176	8060-8064	NN	denotes	tetP
T2177	8064-8065	HYPH	denotes	-
T2178	8065-8067	NN	denotes	mo
T2179	8067-8068	HYPH	denotes	/
T2180	8068-8079	NN	denotes	huAPP695swe
T2181	8079-8080	HYPH	denotes	/
T2182	8095-8105	VBN	denotes	linearized
T2183	8091-8094	VBD	denotes	was
T2184	8106-8109	CC	denotes	and
T2185	8110-8113	DT	denotes	the
T2186	8126-8132	NN	denotes	domain
T2187	8114-8125	NNP	denotes	pBluescript
T2188	8133-8140	VBN	denotes	excised
T2189	8141-8143	IN	denotes	by
T2190	8144-8153	NN	denotes	digestion
T2191	8154-8158	IN	denotes	with
T2192	8159-8163	NN	denotes	NotI
T2193	8163-8164	.	denotes	.
T2194	8164-8270	sentence	denotes	The purified vector was injected into the pronucleus of fertilized eggs from C57BL/6J × C3HeJ F1 matings.
T2195	8165-8168	DT	denotes	The
T2196	8178-8184	NN	denotes	vector
T2197	8169-8177	VBN	denotes	purified
T2198	8189-8197	VBN	denotes	injected
T2199	8185-8188	VBD	denotes	was
T2200	8198-8202	IN	denotes	into
T2201	8203-8206	DT	denotes	the
T2202	8207-8217	NN	denotes	pronucleus
T2203	8218-8220	IN	denotes	of
T2204	8221-8231	VBN	denotes	fertilized
T2205	8232-8236	NNS	denotes	eggs
T2206	8237-8241	IN	denotes	from
T2207	8242-8247	NN	denotes	C57BL
T2208	8253-8258	NN	denotes	C3HeJ
T2209	8247-8248	HYPH	denotes	/
T2210	8248-8250	NN	denotes	6J
T2211	8251-8252	SYM	denotes	×
T2212	8262-8269	NNS	denotes	matings
T2213	8259-8261	NN	denotes	F1
T2214	8269-8270	.	denotes	.
T2215	8270-8407	sentence	denotes	Founder animals were screened for the presence of the transgene by three-way PCR using the S36 and PrP-S/PrP-AS primers described below.
T2216	8271-8278	NN	denotes	Founder
T2217	8279-8286	NNS	denotes	animals
T2218	8292-8300	VBN	denotes	screened
T2219	8287-8291	VBD	denotes	were
T2220	8301-8304	IN	denotes	for
T2221	8305-8308	DT	denotes	the
T2222	8309-8317	NN	denotes	presence
T2223	8318-8320	IN	denotes	of
T2224	8321-8324	DT	denotes	the
T2225	8325-8334	NN	denotes	transgene
T2226	8335-8337	IN	denotes	by
T2227	8338-8343	CD	denotes	three
T2228	8344-8347	NN	denotes	way
T2229	8343-8344	HYPH	denotes	-
T2230	8348-8351	NN	denotes	PCR
T2231	8352-8357	VBG	denotes	using
T2232	8358-8361	DT	denotes	the
T2233	8383-8390	NNS	denotes	primers
T2234	8362-8365	NN	denotes	S36
T2235	8366-8369	CC	denotes	and
T2236	8370-8373	NN	denotes	PrP
T2237	8380-8382	NN	denotes	AS
T2238	8373-8374	HYPH	denotes	-
T2239	8374-8375	NN	denotes	S
T2240	8375-8376	HYPH	denotes	/
T2241	8376-8379	NN	denotes	PrP
T2242	8379-8380	HYPH	denotes	-
T2243	8391-8400	VBN	denotes	described
T2244	8401-8406	RB	denotes	below
T2245	8406-8407	.	denotes	.
T2246	8407-8692	sentence	denotes	Transgene-positive founders were bred to animals expressing the tetracycline transactivator (tTA) under control of the calcium-calmodulin kinase IIα (CaMKIIα) promoter obtained from Jackson Laboratory [16] (Bar Harbor, Maine, United States; stock # 3010; B6;CBA-TgN[Camk2a-tTA]1Mmay).
T2247	8408-8417	NN	denotes	Transgene
T2248	8418-8426	JJ	denotes	positive
T2249	8417-8418	HYPH	denotes	-
T2250	8427-8435	NNS	denotes	founders
T2251	8441-8445	VBN	denotes	bred
T2252	8436-8440	VBD	denotes	were
T2253	8446-8448	IN	denotes	to
T2254	8449-8456	NNS	denotes	animals
T2255	8457-8467	VBG	denotes	expressing
T2256	8468-8471	DT	denotes	the
T2257	8485-8499	NN	denotes	transactivator
T2258	8472-8484	NN	denotes	tetracycline
T2259	8500-8501	-LRB-	denotes	(
T2260	8501-8504	NN	denotes	tTA
T2261	8504-8505	-RRB-	denotes	)
T2262	8506-8511	IN	denotes	under
T2263	8512-8519	NN	denotes	control
T2264	8520-8522	IN	denotes	of
T2265	8523-8526	DT	denotes	the
T2266	8567-8575	NN	denotes	promoter
T2267	8527-8534	NN	denotes	calcium
T2268	8535-8545	NN	denotes	calmodulin
T2269	8534-8535	HYPH	denotes	-
T2270	8553-8556	NN	denotes	IIα
T2271	8546-8552	NN	denotes	kinase
T2272	8557-8558	-LRB-	denotes	(
T2273	8558-8565	NN	denotes	CaMKIIα
T2274	8565-8566	-RRB-	denotes	)
T2275	8576-8584	VBN	denotes	obtained
T2276	8585-8589	IN	denotes	from
T2277	8590-8597	NNP	denotes	Jackson
T2278	8598-8608	NNP	denotes	Laboratory
T2279	8609-8610	-LRB-	denotes	[
T2280	8610-8612	CD	denotes	16
T2281	8612-8613	-RRB-	denotes	]
T2282	8614-8615	-LRB-	denotes	(
T2283	8619-8625	NNP	denotes	Harbor
T2284	8615-8618	NNP	denotes	Bar
T2285	8625-8627	,	denotes	,
T2286	8627-8632	NNP	denotes	Maine
T2287	8632-8634	,	denotes	,
T2288	8634-8640	NNP	denotes	United
T2289	8641-8647	NNP	denotes	States
T2290	8647-8648	:	denotes	;
T2291	8649-8654	NN	denotes	stock
T2292	8685-8690	NN	denotes	1Mmay
T2293	8655-8656	SYM	denotes	#
T2294	8657-8661	CD	denotes	3010
T2295	8661-8662	:	denotes	;
T2296	8663-8665	NN	denotes	B6
T2297	8665-8666	:	denotes	;
T2298	8666-8669	NN	denotes	CBA
T2299	8669-8670	HYPH	denotes	-
T2300	8670-8673	NN	denotes	TgN
T2301	8673-8674	-LRB-	denotes	[
T2302	8674-8680	NN	denotes	Camk2a
T2303	8680-8681	HYPH	denotes	-
T2304	8681-8684	NN	denotes	tTA
T2305	8684-8685	-RRB-	denotes	]
T2306	8690-8691	-RRB-	denotes	)
T2307	8691-8692	.	denotes	.
T2308	8692-8813	sentence	denotes	The colony was thereafter maintained by crossing single transgenic tTA and APP offspring for each of the four APP lines.
T2309	8693-8696	DT	denotes	The
T2310	8697-8703	NN	denotes	colony
T2311	8719-8729	VBN	denotes	maintained
T2312	8704-8707	VBD	denotes	was
T2313	8708-8718	RB	denotes	thereafter
T2314	8730-8732	IN	denotes	by
T2315	8733-8741	VBG	denotes	crossing
T2316	8742-8748	JJ	denotes	single
T2317	8772-8781	NN	denotes	offspring
T2318	8749-8759	JJ	denotes	transgenic
T2319	8760-8763	NN	denotes	tTA
T2320	8764-8767	CC	denotes	and
T2321	8768-8771	NN	denotes	APP
T2322	8782-8785	IN	denotes	for
T2323	8786-8790	DT	denotes	each
T2324	8791-8793	IN	denotes	of
T2325	8794-8797	DT	denotes	the
T2326	8807-8812	NNS	denotes	lines
T2327	8798-8802	CD	denotes	four
T2328	8803-8806	NN	denotes	APP
T2329	8812-8813	.	denotes	.
T2330	8813-8869	sentence	denotes	All mice were provided fresh food and water ad libitum.
T2331	8814-8817	DT	denotes	All
T2332	8818-8822	NNS	denotes	mice
T2333	8828-8836	VBN	denotes	provided
T2334	8823-8827	VBD	denotes	were
T2335	8837-8842	JJ	denotes	fresh
T2336	8843-8847	NN	denotes	food
T2337	8848-8851	CC	denotes	and
T2338	8852-8857	NN	denotes	water
T2339	8858-8860	FW	denotes	ad
T2340	8861-8868	FW	denotes	libitum
T2341	8868-8869	.	denotes	.
T2342	8869-9026	sentence	denotes	Animal protocols were approved by both the Johns Hopkins University and the California Institute of Technology Institutional Animal Care and Use Committees.
T2343	8870-8876	NN	denotes	Animal
T2344	8877-8886	NNS	denotes	protocols
T2345	8892-8900	VBN	denotes	approved
T2346	8887-8891	VBD	denotes	were
T2347	8901-8903	IN	denotes	by
T2348	8904-8908	DT	denotes	both
T2349	8927-8937	NNP	denotes	University
T2350	8909-8912	DT	denotes	the
T2351	8913-8918	NNP	denotes	Johns
T2352	8919-8926	NNP	denotes	Hopkins
T2353	9015-9025	NNPS	denotes	Committees
T2354	8938-8941	CC	denotes	and
T2355	8942-8945	DT	denotes	the
T2356	8957-8966	NNP	denotes	Institute
T2357	8946-8956	NNP	denotes	California
T2358	8967-8969	IN	denotes	of
T2359	8970-8980	NNP	denotes	Technology
T2360	8981-8994	NNP	denotes	Institutional
T2361	8995-9001	NNP	denotes	Animal
T2362	9002-9006	NNP	denotes	Care
T2363	9007-9010	CC	denotes	and
T2364	9011-9014	NNP	denotes	Use
T2365	9025-9026	.	denotes	.
T2408	9028-9039	NN	denotes	Doxycycline
T2409	9040-9054	NN	denotes	Administration
T2410	9054-9190	sentence	denotes	Doxycycline (dox) was administered through commercially available dox-containing chow (BioServ, Frenchtown, New Jersey, United States).
T2411	9055-9066	NN	denotes	Doxycycline
T2412	9077-9089	VBN	denotes	administered
T2413	9067-9068	-LRB-	denotes	(
T2414	9068-9071	NN	denotes	dox
T2415	9071-9072	-RRB-	denotes	)
T2416	9073-9076	VBD	denotes	was
T2417	9090-9097	IN	denotes	through
T2418	9098-9110	RB	denotes	commercially
T2419	9111-9120	JJ	denotes	available
T2420	9136-9140	NN	denotes	chow
T2421	9121-9124	NN	denotes	dox
T2422	9125-9135	VBG	denotes	containing
T2423	9124-9125	HYPH	denotes	-
T2424	9141-9142	-LRB-	denotes	(
T2425	9142-9149	NNP	denotes	BioServ
T2426	9149-9151	,	denotes	,
T2427	9151-9161	NNP	denotes	Frenchtown
T2428	9161-9163	,	denotes	,
T2429	9163-9166	NNP	denotes	New
T2430	9167-9173	NNP	denotes	Jersey
T2431	9173-9175	,	denotes	,
T2432	9175-9181	NNP	denotes	United
T2433	9182-9188	NNP	denotes	States
T2434	9188-9189	-RRB-	denotes	)
T2435	9189-9190	.	denotes	.
T2436	9190-9346	sentence	denotes	The chow contained 200 mg/kg of antibiotic; based on estimated consumption of 5 g per mouse per day, the expected dose to each animal was 1 mg dox per day.
T2437	9191-9194	DT	denotes	The
T2438	9195-9199	NN	denotes	chow
T2439	9200-9209	VBD	denotes	contained
T2440	9325-9328	VBD	denotes	was
T2441	9210-9213	CD	denotes	200
T2442	9214-9216	NN	denotes	mg
T2443	9216-9217	SYM	denotes	/
T2444	9217-9219	NN	denotes	kg
T2445	9220-9222	IN	denotes	of
T2446	9223-9233	JJ	denotes	antibiotic
T2447	9233-9234	:	denotes	;
T2448	9235-9240	VBN	denotes	based
T2449	9241-9243	IN	denotes	on
T2450	9244-9253	VBN	denotes	estimated
T2451	9254-9265	NN	denotes	consumption
T2452	9266-9268	IN	denotes	of
T2453	9269-9270	CD	denotes	5
T2454	9271-9272	NN	denotes	g
T2455	9273-9276	IN	denotes	per
T2456	9277-9282	NN	denotes	mouse
T2457	9283-9286	IN	denotes	per
T2458	9287-9290	NN	denotes	day
T2459	9290-9292	,	denotes	,
T2460	9292-9295	DT	denotes	the
T2461	9305-9309	NN	denotes	dose
T2462	9296-9304	VBN	denotes	expected
T2463	9310-9312	IN	denotes	to
T2464	9313-9317	DT	denotes	each
T2465	9318-9324	NN	denotes	animal
T2466	9329-9330	CD	denotes	1
T2467	9331-9333	NN	denotes	mg
T2468	9334-9337	NN	denotes	dox
T2469	9338-9341	IN	denotes	per
T2470	9342-9345	NN	denotes	day
T2471	9345-9346	.	denotes	.
T2472	9346-9432	sentence	denotes	The average 25-g animal therefore received 40 μg of dox per gram body weight per day.
T2473	9347-9350	DT	denotes	The
T2474	9364-9370	NN	denotes	animal
T2475	9351-9358	JJ	denotes	average
T2476	9359-9361	CD	denotes	25
T2477	9362-9363	NN	denotes	g
T2478	9361-9362	HYPH	denotes	-
T2479	9381-9389	VBD	denotes	received
T2480	9371-9380	RB	denotes	therefore
T2481	9390-9392	CD	denotes	40
T2482	9393-9395	NN	denotes	μg
T2483	9396-9398	IN	denotes	of
T2484	9399-9402	NN	denotes	dox
T2485	9403-9406	IN	denotes	per
T2486	9407-9411	NN	denotes	gram
T2487	9417-9423	NN	denotes	weight
T2488	9412-9416	NN	denotes	body
T2489	9424-9427	IN	denotes	per
T2490	9428-9431	NN	denotes	day
T2491	9431-9432	.	denotes	.
T2492	9432-9508	sentence	denotes	Chow was changed 1–2 times per week to prevent breakdown of the antibiotic.
T2493	9433-9437	NN	denotes	Chow
T2494	9442-9449	VBN	denotes	changed
T2495	9438-9441	VBD	denotes	was
T2496	9450-9451	CD	denotes	1
T2497	9452-9453	CD	denotes	2
T2498	9451-9452	SYM	denotes	–
T2499	9454-9459	NNS	denotes	times
T2500	9460-9463	IN	denotes	per
T2501	9464-9468	NN	denotes	week
T2502	9469-9471	TO	denotes	to
T2503	9472-9479	VB	denotes	prevent
T2504	9480-9489	NN	denotes	breakdown
T2505	9490-9492	IN	denotes	of
T2506	9493-9496	DT	denotes	the
T2507	9497-9507	NN	denotes	antibiotic
T2508	9507-9508	.	denotes	.
T2635	9510-9520	NN	denotes	Genotyping
T2636	9520-9651	sentence	denotes	Offspring were genotyped for the presence of each transgene by PCR amplification of genomic DNA extracted from a 5-mm tail biopsy.
T2637	9521-9530	NN	denotes	Offspring
T2638	9536-9545	VBN	denotes	genotyped
T2639	9531-9535	VBD	denotes	were
T2640	9546-9549	IN	denotes	for
T2641	9550-9553	DT	denotes	the
T2642	9554-9562	NN	denotes	presence
T2643	9563-9565	IN	denotes	of
T2644	9566-9570	DT	denotes	each
T2645	9571-9580	NN	denotes	transgene
T2646	9581-9583	IN	denotes	by
T2647	9584-9587	NN	denotes	PCR
T2648	9588-9601	NN	denotes	amplification
T2649	9602-9604	IN	denotes	of
T2650	9605-9612	JJ	denotes	genomic
T2651	9613-9616	NN	denotes	DNA
T2652	9617-9626	VBN	denotes	extracted
T2653	9627-9631	IN	denotes	from
T2654	9632-9633	DT	denotes	a
T2655	9644-9650	NN	denotes	biopsy
T2656	9634-9635	CD	denotes	5
T2657	9636-9638	NN	denotes	mm
T2658	9635-9636	HYPH	denotes	-
T2659	9639-9643	NN	denotes	tail
T2660	9650-9651	.	denotes	.
T2661	9651-9790	sentence	denotes	Tails were heated to 95 °C for 45 min in 250 μl of 50 mM NaOH, vortexed, then neutralized with an equal volume of 0.5 M Tris-HCl (pH 5.5).
T2662	9652-9657	NNS	denotes	Tails
T2663	9663-9669	VBN	denotes	heated
T2664	9658-9662	VBD	denotes	were
T2665	9670-9672	IN	denotes	to
T2666	9673-9675	CD	denotes	95
T2667	9676-9678	NN	denotes	°C
T2668	9679-9682	IN	denotes	for
T2669	9683-9685	CD	denotes	45
T2670	9686-9689	NN	denotes	min
T2671	9690-9692	IN	denotes	in
T2672	9693-9696	CD	denotes	250
T2673	9697-9699	NN	denotes	μl
T2674	9700-9702	IN	denotes	of
T2675	9703-9705	CD	denotes	50
T2676	9706-9708	NN	denotes	mM
T2677	9709-9713	NN	denotes	NaOH
T2678	9713-9715	,	denotes	,
T2679	9715-9723	VBN	denotes	vortexed
T2680	9723-9725	,	denotes	,
T2681	9725-9729	RB	denotes	then
T2682	9730-9741	VBN	denotes	neutralized
T2683	9742-9746	IN	denotes	with
T2684	9747-9749	DT	denotes	an
T2685	9756-9762	NN	denotes	volume
T2686	9750-9755	JJ	denotes	equal
T2687	9763-9765	IN	denotes	of
T2688	9766-9769	CD	denotes	0.5
T2689	9770-9771	NN	denotes	M
T2690	9777-9780	NN	denotes	HCl
T2691	9772-9776	NN	denotes	Tris
T2692	9776-9777	HYPH	denotes	-
T2693	9781-9782	-LRB-	denotes	(
T2694	9782-9784	NN	denotes	pH
T2695	9785-9788	CD	denotes	5.5
T2696	9788-9789	-RRB-	denotes	)
T2697	9789-9790	.	denotes	.
T2698	9790-9883	sentence	denotes	Debris was sedimented by centrifugation, and 3 μl of supernatant was used for amplification.
T2699	9791-9797	NN	denotes	Debris
T2700	9802-9812	VBN	denotes	sedimented
T2701	9798-9801	VBD	denotes	was
T2702	9813-9815	IN	denotes	by
T2703	9816-9830	NN	denotes	centrifugation
T2704	9830-9832	,	denotes	,
T2705	9832-9835	CC	denotes	and
T2706	9836-9837	CD	denotes	3
T2707	9838-9840	NN	denotes	μl
T2708	9860-9864	VBN	denotes	used
T2709	9841-9843	IN	denotes	of
T2710	9844-9855	NN	denotes	supernatant
T2711	9856-9859	VBD	denotes	was
T2712	9865-9868	IN	denotes	for
T2713	9869-9882	NN	denotes	amplification
T2714	9882-9883	.	denotes	.
T2715	9883-9990	sentence	denotes	Genotyping for APP and tTA transgenes was performed in the same PCR reaction, using five separate primers.
T2716	9884-9894	VBG	denotes	Genotyping
T2717	9926-9935	VBN	denotes	performed
T2718	9895-9898	IN	denotes	for
T2719	9899-9902	NN	denotes	APP
T2720	9911-9921	NNS	denotes	transgenes
T2721	9903-9906	CC	denotes	and
T2722	9907-9910	NN	denotes	tTA
T2723	9922-9925	VBD	denotes	was
T2724	9936-9938	IN	denotes	in
T2725	9939-9942	DT	denotes	the
T2726	9952-9960	NN	denotes	reaction
T2727	9943-9947	JJ	denotes	same
T2728	9948-9951	NN	denotes	PCR
T2729	9960-9962	,	denotes	,
T2730	9962-9967	VBG	denotes	using
T2731	9968-9972	CD	denotes	five
T2732	9982-9989	NNS	denotes	primers
T2733	9973-9981	JJ	denotes	separate
T2734	9989-9990	.	denotes	.
T2735	9990-10207	sentence	denotes	APP was amplified using forward primer S36 located in the 3′ end of the APP cDNA (CCG AGA TCT CTG AAG TGA AGA TGG ATG) and reverse primer PrP-AS-J located in the 3′ UTR of the vector (CCA AGC CTA GAC CAC GAG AAT GC).
T2736	9991-9994	NN	denotes	APP
T2737	9999-10008	VBN	denotes	amplified
T2738	9995-9998	VBD	denotes	was
T2739	10009-10014	VBG	denotes	using
T2740	10015-10022	RB	denotes	forward
T2741	10030-10033	NN	denotes	S36
T2742	10023-10029	NN	denotes	primer
T2743	10034-10041	VBN	denotes	located
T2744	10042-10044	IN	denotes	in
T2745	10045-10048	DT	denotes	the
T2746	10052-10055	NN	denotes	end
T2747	10049-10050	CD	denotes	3
T2748	10050-10051	SYM	denotes	′
T2749	10056-10058	IN	denotes	of
T2750	10059-10062	DT	denotes	the
T2751	10067-10071	NN	denotes	cDNA
T2752	10063-10066	NN	denotes	APP
T2753	10072-10073	-LRB-	denotes	(
T2754	10105-10108	NN	denotes	ATG
T2755	10073-10076	NN	denotes	CCG
T2756	10077-10080	NN	denotes	AGA
T2757	10081-10084	NN	denotes	TCT
T2758	10085-10088	NN	denotes	CTG
T2759	10089-10092	NN	denotes	AAG
T2760	10093-10096	NN	denotes	TGA
T2761	10097-10100	NN	denotes	AGA
T2762	10101-10104	NN	denotes	TGG
T2763	10108-10109	-RRB-	denotes	)
T2764	10110-10113	CC	denotes	and
T2765	10114-10121	JJ	denotes	reverse
T2766	10136-10137	NN	denotes	J
T2767	10122-10128	NN	denotes	primer
T2768	10129-10132	NN	denotes	PrP
T2769	10132-10133	HYPH	denotes	-
T2770	10133-10135	NN	denotes	AS
T2771	10135-10136	HYPH	denotes	-
T2772	10138-10145	VBN	denotes	located
T2773	10146-10148	IN	denotes	in
T2774	10149-10152	DT	denotes	the
T2775	10156-10159	NN	denotes	UTR
T2776	10153-10154	CD	denotes	3
T2777	10154-10155	SYM	denotes	′
T2778	10160-10162	IN	denotes	of
T2779	10163-10166	DT	denotes	the
T2780	10167-10173	NN	denotes	vector
T2781	10174-10175	-LRB-	denotes	(
T2782	10203-10205	NN	denotes	GC
T2783	10175-10178	NN	denotes	CCA
T2784	10179-10182	NN	denotes	AGC
T2785	10183-10186	NN	denotes	CTA
T2786	10187-10190	NN	denotes	GAC
T2787	10191-10194	NN	denotes	CAC
T2788	10195-10198	NN	denotes	GAG
T2789	10199-10202	NN	denotes	AAT
T2790	10205-10206	-RRB-	denotes	)
T2791	10206-10207	.	denotes	.
T2792	10207-10463	sentence	denotes	The tTA transgene was detected using a primer set that amplified across its two subdomains with tet forward located within the Tn10 tetracycline repressor (CGC TGT GGG GCA TTT TAC TTT AG) and tet reverse within the HSV1 VP16 (CAT GTC CAG ATC GAA ATC GTC).
T2793	10208-10211	DT	denotes	The
T2794	10216-10225	NN	denotes	transgene
T2795	10212-10215	NN	denotes	tTA
T2796	10230-10238	VBN	denotes	detected
T2797	10226-10229	VBD	denotes	was
T2798	10239-10244	VBG	denotes	using
T2799	10245-10246	DT	denotes	a
T2800	10254-10257	NN	denotes	set
T2801	10247-10253	NN	denotes	primer
T2802	10258-10262	WDT	denotes	that
T2803	10263-10272	VBD	denotes	amplified
T2804	10273-10279	IN	denotes	across
T2805	10280-10283	PRP$	denotes	its
T2806	10288-10298	NNS	denotes	subdomains
T2807	10284-10287	CD	denotes	two
T2808	10299-10303	IN	denotes	with
T2809	10304-10307	NN	denotes	tet
T2810	10308-10315	RB	denotes	forward
T2811	10316-10323	VBN	denotes	located
T2812	10324-10330	IN	denotes	within
T2813	10331-10334	DT	denotes	the
T2814	10353-10362	NN	denotes	repressor
T2815	10335-10339	NN	denotes	Tn10
T2816	10340-10352	NN	denotes	tetracycline
T2817	10363-10364	-LRB-	denotes	(
T2818	10392-10394	NN	denotes	AG
T2819	10364-10367	NN	denotes	CGC
T2820	10368-10371	NN	denotes	TGT
T2821	10372-10375	NN	denotes	GGG
T2822	10376-10379	NN	denotes	GCA
T2823	10380-10383	NN	denotes	TTT
T2824	10384-10387	NN	denotes	TAC
T2825	10388-10391	NN	denotes	TTT
T2826	10394-10395	-RRB-	denotes	)
T2827	10396-10399	CC	denotes	and
T2828	10400-10403	NN	denotes	tet
T2829	10404-10411	JJ	denotes	reverse
T2830	10412-10418	IN	denotes	within
T2831	10419-10422	DT	denotes	the
T2832	10428-10432	NN	denotes	VP16
T2833	10423-10427	NN	denotes	HSV1
T2834	10433-10434	-LRB-	denotes	(
T2835	10458-10461	NN	denotes	GTC
T2836	10434-10437	NN	denotes	CAT
T2837	10438-10441	NN	denotes	GTC
T2838	10442-10445	NN	denotes	CAG
T2839	10446-10449	NN	denotes	ATC
T2840	10450-10453	NN	denotes	GAA
T2841	10454-10457	NN	denotes	ATC
T2842	10461-10462	-RRB-	denotes	)
T2843	10462-10463	.	denotes	.
T2844	10463-10816	sentence	denotes	All reactions, whether transgene-positive or not, amplified a segment of the endogenous prion protein gene as a control for DNA quality using a forward primer, PrP-S-J, specific to the mouse PrP open reading frame (GGG ACT ATG TGG ACT GAT GTC GG) and a reverse primer, PrP-AS-J, shared by the 3′ UTR of the endogenous PrP gene and the transgene vector.
T2845	10464-10467	DT	denotes	All
T2846	10468-10477	NNS	denotes	reactions
T2847	10514-10523	VBD	denotes	amplified
T2848	10477-10479	,	denotes	,
T2849	10479-10486	IN	denotes	whether
T2850	10497-10505	JJ	denotes	positive
T2851	10487-10496	NN	denotes	transgene
T2852	10496-10497	HYPH	denotes	-
T2853	10506-10508	CC	denotes	or
T2854	10509-10512	RB	denotes	not
T2855	10512-10514	,	denotes	,
T2856	10524-10525	DT	denotes	a
T2857	10526-10533	NN	denotes	segment
T2858	10534-10536	IN	denotes	of
T2859	10537-10540	DT	denotes	the
T2860	10566-10570	NN	denotes	gene
T2861	10541-10551	JJ	denotes	endogenous
T2862	10552-10557	NN	denotes	prion
T2863	10558-10565	NN	denotes	protein
T2864	10571-10573	IN	denotes	as
T2865	10574-10575	DT	denotes	a
T2866	10576-10583	NN	denotes	control
T2867	10584-10587	IN	denotes	for
T2868	10588-10591	NN	denotes	DNA
T2869	10592-10599	NN	denotes	quality
T2870	10600-10605	VBG	denotes	using
T2871	10606-10607	DT	denotes	a
T2872	10616-10622	NN	denotes	primer
T2873	10608-10615	JJ	denotes	forward
T2874	10622-10624	,	denotes	,
T2875	10624-10627	NN	denotes	PrP
T2876	10630-10631	NN	denotes	J
T2877	10627-10628	HYPH	denotes	-
T2878	10628-10629	NN	denotes	S
T2879	10629-10630	HYPH	denotes	-
T2880	10631-10633	,	denotes	,
T2881	10633-10641	JJ	denotes	specific
T2882	10642-10644	IN	denotes	to
T2883	10645-10648	DT	denotes	the
T2884	10672-10677	NN	denotes	frame
T2885	10649-10654	NN	denotes	mouse
T2886	10655-10658	NN	denotes	PrP
T2887	10659-10663	JJ	denotes	open
T2888	10664-10671	NN	denotes	reading
T2889	10678-10679	-LRB-	denotes	(
T2890	10707-10709	NN	denotes	GG
T2891	10679-10682	NN	denotes	GGG
T2892	10683-10686	NN	denotes	ACT
T2893	10687-10690	NN	denotes	ATG
T2894	10691-10694	NN	denotes	TGG
T2895	10695-10698	NN	denotes	ACT
T2896	10699-10702	NN	denotes	GAT
T2897	10703-10706	NN	denotes	GTC
T2898	10709-10710	-RRB-	denotes	)
T2899	10711-10714	CC	denotes	and
T2900	10715-10716	DT	denotes	a
T2901	10725-10731	NN	denotes	primer
T2902	10717-10724	JJ	denotes	reverse
T2903	10731-10733	,	denotes	,
T2904	10733-10736	NN	denotes	PrP
T2905	10740-10741	NN	denotes	J
T2906	10736-10737	HYPH	denotes	-
T2907	10737-10739	NN	denotes	AS
T2908	10739-10740	HYPH	denotes	-
T2909	10741-10743	,	denotes	,
T2910	10743-10749	VBN	denotes	shared
T2911	10750-10752	IN	denotes	by
T2912	10753-10756	DT	denotes	the
T2913	10760-10763	NN	denotes	UTR
T2914	10757-10758	CD	denotes	3
T2915	10758-10759	SYM	denotes	′
T2916	10764-10766	IN	denotes	of
T2917	10767-10770	DT	denotes	the
T2918	10786-10790	NN	denotes	gene
T2919	10771-10781	JJ	denotes	endogenous
T2920	10782-10785	NN	denotes	PrP
T2921	10791-10794	CC	denotes	and
T2922	10795-10798	DT	denotes	the
T2923	10809-10815	NN	denotes	vector
T2924	10799-10808	NN	denotes	transgene
T2925	10815-10816	.	denotes	.
T2926	10816-10920	sentence	denotes	Amplification reactions were run for 37 cycles at 94 °C for 30 s, 64 °C for 1 min, and 72 °C for 1 min.
T2927	10817-10830	NN	denotes	Amplification
T2928	10831-10840	NNS	denotes	reactions
T2929	10846-10849	VBN	denotes	run
T2930	10841-10845	VBD	denotes	were
T2931	10850-10853	IN	denotes	for
T2932	10854-10856	CD	denotes	37
T2933	10857-10863	NNS	denotes	cycles
T2934	10864-10866	IN	denotes	at
T2935	10867-10869	CD	denotes	94
T2936	10870-10872	NN	denotes	°C
T2937	10873-10876	IN	denotes	for
T2938	10877-10879	CD	denotes	30
T2939	10880-10881	NN	denotes	s
T2940	10881-10883	,	denotes	,
T2941	10883-10885	CD	denotes	64
T2942	10886-10888	NN	denotes	°C
T2943	10889-10892	IN	denotes	for
T2944	10893-10894	CD	denotes	1
T2945	10895-10898	NN	denotes	min
T2946	10898-10900	,	denotes	,
T2947	10900-10903	CC	denotes	and
T2948	10904-10906	CD	denotes	72
T2949	10907-10909	NN	denotes	°C
T2950	10910-10913	IN	denotes	for
T2951	10914-10915	CD	denotes	1
T2952	10916-10919	NN	denotes	min
T2953	10919-10920	.	denotes	.
T2954	10920-11011	sentence	denotes	All samples, transgenic and wild-type, gave a 750-bp product from the endogenous PrP gene.
T2955	10921-10924	DT	denotes	All
T2956	10925-10932	NNS	denotes	samples
T2957	10960-10964	VBD	denotes	gave
T2958	10932-10934	,	denotes	,
T2959	10934-10944	JJ	denotes	transgenic
T2960	10945-10948	CC	denotes	and
T2961	10949-10953	JJ	denotes	wild
T2962	10954-10958	NN	denotes	type
T2963	10953-10954	HYPH	denotes	-
T2964	10958-10960	,	denotes	,
T2965	10965-10966	DT	denotes	a
T2966	10974-10981	NN	denotes	product
T2967	10967-10970	CD	denotes	750
T2968	10971-10973	NN	denotes	bp
T2969	10970-10971	HYPH	denotes	-
T2970	10982-10986	IN	denotes	from
T2971	10987-10990	DT	denotes	the
T2972	11006-11010	NN	denotes	gene
T2973	10991-11001	JJ	denotes	endogenous
T2974	11002-11005	NN	denotes	PrP
T2975	11010-11011	.	denotes	.
T2976	11011-11110	sentence	denotes	The APP transgene yielded an additional band at 400 bp; the tTA product fell in between at 480 bp.
T2977	11012-11015	DT	denotes	The
T2978	11020-11029	NN	denotes	transgene
T2979	11016-11019	NN	denotes	APP
T2980	11030-11037	VBD	denotes	yielded
T2981	11084-11088	VBD	denotes	fell
T2982	11038-11040	DT	denotes	an
T2983	11052-11056	NN	denotes	band
T2984	11041-11051	JJ	denotes	additional
T2985	11057-11059	IN	denotes	at
T2986	11060-11063	CD	denotes	400
T2987	11064-11066	NN	denotes	bp
T2988	11066-11067	:	denotes	;
T2989	11068-11071	DT	denotes	the
T2990	11076-11083	NN	denotes	product
T2991	11072-11075	NN	denotes	tTA
T2992	11089-11091	RB	denotes	in
T2993	11092-11099	RB	denotes	between
T2994	11100-11102	IN	denotes	at
T2995	11103-11106	CD	denotes	480
T2996	11107-11109	NN	denotes	bp
T2997	11109-11110	.	denotes	.
T3141	11112-11126	NN	denotes	Immunoblotting
T3142	11127-11139	NN	denotes	Quantitation
T3143	11126-11127	HYPH	denotes	/
T3144	11139-11456	sentence	denotes	Frozen cortical or whole forebrain tissue was homogenized by sonication in five volumes of phosphate-buffered saline (PBS) with 5 mM EDTA and protease inhibitors (Mammalian cell cocktail, Sigma, St. Louis, Missouri, United States), using a probe sonicator set to 50% output (TEKMAR, Cincinnati, Ohio, United States).
T3145	11140-11146	VBN	denotes	Frozen
T3146	11175-11181	NN	denotes	tissue
T3147	11147-11155	JJ	denotes	cortical
T3148	11156-11158	CC	denotes	or
T3149	11159-11164	JJ	denotes	whole
T3150	11165-11174	NN	denotes	forebrain
T3151	11186-11197	VBN	denotes	homogenized
T3152	11182-11185	VBD	denotes	was
T3153	11198-11200	IN	denotes	by
T3154	11201-11211	NN	denotes	sonication
T3155	11212-11214	IN	denotes	in
T3156	11215-11219	CD	denotes	five
T3157	11220-11227	NNS	denotes	volumes
T3158	11228-11230	IN	denotes	of
T3159	11231-11240	NN	denotes	phosphate
T3160	11241-11249	VBN	denotes	buffered
T3161	11240-11241	HYPH	denotes	-
T3162	11250-11256	NN	denotes	saline
T3163	11257-11258	-LRB-	denotes	(
T3164	11258-11261	NN	denotes	PBS
T3165	11261-11262	-RRB-	denotes	)
T3166	11263-11267	IN	denotes	with
T3167	11268-11269	CD	denotes	5
T3168	11270-11272	NN	denotes	mM
T3169	11291-11301	NNS	denotes	inhibitors
T3170	11273-11277	NN	denotes	EDTA
T3171	11278-11281	CC	denotes	and
T3172	11282-11290	NN	denotes	protease
T3173	11302-11303	-LRB-	denotes	(
T3174	11318-11326	NN	denotes	cocktail
T3175	11303-11312	JJ	denotes	Mammalian
T3176	11313-11317	NN	denotes	cell
T3177	11326-11328	,	denotes	,
T3178	11328-11333	NNP	denotes	Sigma
T3179	11333-11335	,	denotes	,
T3180	11335-11338	NNP	denotes	St.
T3181	11339-11344	NNP	denotes	Louis
T3182	11344-11346	,	denotes	,
T3183	11346-11354	NNP	denotes	Missouri
T3184	11354-11356	,	denotes	,
T3185	11356-11362	NNP	denotes	United
T3186	11363-11369	NNP	denotes	States
T3187	11369-11370	-RRB-	denotes	)
T3188	11370-11372	,	denotes	,
T3189	11372-11377	VBG	denotes	using
T3190	11378-11379	DT	denotes	a
T3191	11386-11395	NN	denotes	sonicator
T3192	11380-11385	NN	denotes	probe
T3193	11396-11399	VBN	denotes	set
T3194	11400-11402	IN	denotes	to
T3195	11403-11405	CD	denotes	50
T3196	11405-11406	NN	denotes	%
T3197	11407-11413	NN	denotes	output
T3198	11414-11415	-LRB-	denotes	(
T3199	11415-11421	NNP	denotes	TEKMAR
T3200	11421-11423	,	denotes	,
T3201	11423-11433	NNP	denotes	Cincinnati
T3202	11433-11435	,	denotes	,
T3203	11435-11439	NNP	denotes	Ohio
T3204	11439-11441	,	denotes	,
T3205	11441-11447	NNP	denotes	United
T3206	11448-11454	NNP	denotes	States
T3207	11454-11455	-RRB-	denotes	)
T3208	11455-11456	.	denotes	.
T3209	11456-11600	sentence	denotes	After dilution with an equal volume of PBS/EDTA/protease inhibitor, the samples were centrifuged briefly and the supernatant used for analysis.
T3210	11457-11462	IN	denotes	After
T3211	11542-11553	VBN	denotes	centrifuged
T3212	11463-11471	NN	denotes	dilution
T3213	11472-11476	IN	denotes	with
T3214	11477-11479	DT	denotes	an
T3215	11486-11492	NN	denotes	volume
T3216	11480-11485	JJ	denotes	equal
T3217	11493-11495	IN	denotes	of
T3218	11496-11499	NN	denotes	PBS
T3219	11505-11513	NN	denotes	protease
T3220	11499-11500	HYPH	denotes	/
T3221	11500-11504	NN	denotes	EDTA
T3222	11504-11505	HYPH	denotes	/
T3223	11514-11523	NN	denotes	inhibitor
T3224	11523-11525	,	denotes	,
T3225	11525-11528	DT	denotes	the
T3226	11529-11536	NNS	denotes	samples
T3227	11537-11541	VBD	denotes	were
T3228	11554-11561	RB	denotes	briefly
T3229	11562-11565	CC	denotes	and
T3230	11566-11569	DT	denotes	the
T3231	11570-11581	NN	denotes	supernatant
T3232	11582-11586	VBN	denotes	used
T3233	11587-11590	IN	denotes	for
T3234	11591-11599	NN	denotes	analysis
T3235	11599-11600	.	denotes	.
T3236	11600-12037	sentence	denotes	Fifty micrograms (6E10 and CT15) or 5 μg (22C11) of brain homogenate was loaded per lane onto 7.5%, 10%–20%, or 4%–20% Tris-HCl PAGE gels (Bio-Rad Laboratories, Hercules, California, United States) and electrophoresed for several hours in 1× Tris-glycine–sodium dodecyl sulfate (1×TG-SDS) buffer (6E10 and 22C11; Amresco, Solon, Ohio, United States) or 1× Tris-tricine-SDS buffer (CT15; Invitrogen, Carlsbad, California, United States).
T3237	11601-11606	CD	denotes	Fifty
T3238	11607-11617	NNS	denotes	micrograms
T3239	11619-11623	NN	denotes	6E10
T3240	11618-11619	-LRB-	denotes	(
T3241	11674-11680	VBN	denotes	loaded
T3242	11624-11627	CC	denotes	and
T3243	11628-11632	NN	denotes	CT15
T3244	11632-11633	-RRB-	denotes	)
T3245	11634-11636	CC	denotes	or
T3246	11637-11638	CD	denotes	5
T3247	11639-11641	NN	denotes	μg
T3248	11643-11648	NN	denotes	22C11
T3249	11642-11643	-LRB-	denotes	(
T3250	11648-11649	-RRB-	denotes	)
T3251	11650-11652	IN	denotes	of
T3252	11653-11658	NN	denotes	brain
T3253	11659-11669	NN	denotes	homogenate
T3254	11670-11673	VBD	denotes	was
T3255	11681-11684	IN	denotes	per
T3256	11685-11689	NN	denotes	lane
T3257	11690-11694	IN	denotes	onto
T3258	11695-11698	CD	denotes	7.5
T3259	11698-11699	NN	denotes	%
T3260	11729-11733	NN	denotes	PAGE
T3261	11699-11701	,	denotes	,
T3262	11701-11703	CD	denotes	10
T3263	11703-11704	NN	denotes	%
T3264	11704-11705	SYM	denotes	–
T3265	11707-11708	NN	denotes	%
T3266	11705-11707	CD	denotes	20
T3267	11708-11710	,	denotes	,
T3268	11710-11712	CC	denotes	or
T3269	11713-11714	CD	denotes	4
T3270	11714-11715	NN	denotes	%
T3271	11715-11716	SYM	denotes	–
T3272	11718-11719	NN	denotes	%
T3273	11716-11718	CD	denotes	20
T3274	11720-11724	NN	denotes	Tris
T3275	11724-11725	HYPH	denotes	-
T3276	11725-11728	NN	denotes	HCl
T3277	11734-11738	NNS	denotes	gels
T3278	11739-11740	-LRB-	denotes	(
T3279	11748-11760	NNP	denotes	Laboratories
T3280	11740-11743	NNP	denotes	Bio
T3281	11744-11747	NNP	denotes	Rad
T3282	11743-11744	HYPH	denotes	-
T3283	11760-11762	,	denotes	,
T3284	11762-11770	NNP	denotes	Hercules
T3285	11770-11772	,	denotes	,
T3286	11772-11782	NNP	denotes	California
T3287	11782-11784	,	denotes	,
T3288	11784-11790	NNP	denotes	United
T3289	11791-11797	NNP	denotes	States
T3290	11797-11798	-RRB-	denotes	)
T3291	11799-11802	CC	denotes	and
T3292	11803-11818	VBN	denotes	electrophoresed
T3293	11819-11822	IN	denotes	for
T3294	11823-11830	JJ	denotes	several
T3295	11831-11836	NNS	denotes	hours
T3296	11837-11839	IN	denotes	in
T3297	11840-11841	CD	denotes	1
T3298	11871-11878	NN	denotes	sulfate
T3299	11841-11842	SYM	denotes	×
T3300	11843-11847	NN	denotes	Tris
T3301	11847-11848	HYPH	denotes	-
T3302	11848-11855	NN	denotes	glycine
T3303	11855-11856	HYPH	denotes	–
T3304	11856-11862	NN	denotes	sodium
T3305	11863-11870	NN	denotes	dodecyl
T3306	11890-11896	NN	denotes	buffer
T3307	11879-11880	-LRB-	denotes	(
T3308	11880-11881	CD	denotes	1
T3309	11885-11888	NN	denotes	SDS
T3310	11881-11882	SYM	denotes	×
T3311	11882-11884	NN	denotes	TG
T3312	11884-11885	HYPH	denotes	-
T3313	11888-11889	-RRB-	denotes	)
T3314	11897-11898	-LRB-	denotes	(
T3315	11914-11921	NNP	denotes	Amresco
T3316	11898-11902	NN	denotes	6E10
T3317	11903-11906	CC	denotes	and
T3318	11907-11912	NN	denotes	22C11
T3319	11912-11913	:	denotes	;
T3320	11921-11923	,	denotes	,
T3321	11923-11928	NNP	denotes	Solon
T3322	11928-11930	,	denotes	,
T3323	11930-11934	NNP	denotes	Ohio
T3324	11934-11936	,	denotes	,
T3325	11936-11942	NNP	denotes	United
T3326	11943-11949	NNP	denotes	States
T3327	11949-11950	-RRB-	denotes	)
T3328	11951-11953	CC	denotes	or
T3329	11954-11955	CD	denotes	1
T3330	11974-11980	NN	denotes	buffer
T3331	11955-11956	SYM	denotes	×
T3332	11957-11961	NN	denotes	Tris
T3333	11970-11973	NN	denotes	SDS
T3334	11961-11962	HYPH	denotes	-
T3335	11962-11969	NN	denotes	tricine
T3336	11969-11970	HYPH	denotes	-
T3337	11981-11982	-LRB-	denotes	(
T3338	11988-11998	NNP	denotes	Invitrogen
T3339	11982-11986	NN	denotes	CT15
T3340	11986-11987	:	denotes	;
T3341	11998-12000	,	denotes	,
T3342	12000-12008	NNP	denotes	Carlsbad
T3343	12008-12010	,	denotes	,
T3344	12010-12020	NNP	denotes	California
T3345	12020-12022	,	denotes	,
T3346	12022-12028	NNP	denotes	United
T3347	12029-12035	NNP	denotes	States
T3348	12035-12036	-RRB-	denotes	)
T3349	12036-12037	.	denotes	.
T3350	12037-12197	sentence	denotes	Proteins were transferred overnight to 0.45-μm Optitran nitrocellulose (Schleicher and Schuell, Keene, New Hampshire, United States) in 1× TG buffer (Amresco).
T3351	12038-12046	NNS	denotes	Proteins
T3352	12052-12063	VBN	denotes	transferred
T3353	12047-12051	VBD	denotes	were
T3354	12064-12073	RB	denotes	overnight
T3355	12074-12076	IN	denotes	to
T3356	12077-12081	CD	denotes	0.45
T3357	12082-12084	NN	denotes	μm
T3358	12081-12082	HYPH	denotes	-
T3359	12094-12108	NN	denotes	nitrocellulose
T3360	12085-12093	NNP	denotes	Optitran
T3361	12109-12110	-LRB-	denotes	(
T3362	12110-12120	NNP	denotes	Schleicher
T3363	12121-12124	CC	denotes	and
T3364	12125-12132	NNP	denotes	Schuell
T3365	12132-12134	,	denotes	,
T3366	12134-12139	NNP	denotes	Keene
T3367	12139-12141	,	denotes	,
T3368	12141-12144	NNP	denotes	New
T3369	12145-12154	NNP	denotes	Hampshire
T3370	12154-12156	,	denotes	,
T3371	12156-12162	NNP	denotes	United
T3372	12163-12169	NNP	denotes	States
T3373	12169-12170	-RRB-	denotes	)
T3374	12171-12173	IN	denotes	in
T3375	12174-12175	CD	denotes	1
T3376	12180-12186	NN	denotes	buffer
T3377	12175-12176	SYM	denotes	×
T3378	12177-12179	NN	denotes	TG
T3379	12187-12188	-LRB-	denotes	(
T3380	12188-12195	NNP	denotes	Amresco
T3381	12195-12196	-RRB-	denotes	)
T3382	12196-12197	.	denotes	.
T3383	12197-12722	sentence	denotes	Blots were blocked in PBS containing 5% nonfat dry milk powder, and incubated for 3 h at room temperature in blocking solution with one of the following antibodies: mouse monoclonal 22C11 (kind gift of Konrad Beyreuther and Andreas Weidemann; [17]) diluted 1:1,000, mouse monoclonal 6E10 (Signet Laboratories, Dedham, Massachusetts, United States) diluted 1:2,500, rabbit polyclonal anti-superoxide dismutase 1 (m/hSOD1) [18] diluted 1:2,500 to 1:4,000, or rabbit polyclonal CT15 (kind gift of Ed Koo; [19]) diluted 1:1,000.
T3384	12198-12203	NNS	denotes	Blots
T3385	12209-12216	VBN	denotes	blocked
T3386	12204-12208	VBD	denotes	were
T3387	12217-12219	IN	denotes	in
T3388	12220-12223	NN	denotes	PBS
T3389	12224-12234	VBG	denotes	containing
T3390	12235-12236	CD	denotes	5
T3391	12236-12237	NN	denotes	%
T3392	12254-12260	NN	denotes	powder
T3393	12238-12244	JJ	denotes	nonfat
T3394	12249-12253	NN	denotes	milk
T3395	12245-12248	JJ	denotes	dry
T3396	12260-12262	,	denotes	,
T3397	12262-12265	CC	denotes	and
T3398	12266-12275	VBN	denotes	incubated
T3399	12276-12279	IN	denotes	for
T3400	12280-12281	CD	denotes	3
T3401	12282-12283	NN	denotes	h
T3402	12284-12286	IN	denotes	at
T3403	12287-12291	NN	denotes	room
T3404	12292-12303	NN	denotes	temperature
T3405	12304-12306	IN	denotes	in
T3406	12307-12315	NN	denotes	blocking
T3407	12316-12324	NN	denotes	solution
T3408	12325-12329	IN	denotes	with
T3409	12330-12333	CD	denotes	one
T3410	12334-12336	IN	denotes	of
T3411	12337-12340	DT	denotes	the
T3412	12351-12361	NNS	denotes	antibodies
T3413	12341-12350	VBG	denotes	following
T3414	12361-12363	:	denotes	:
T3415	12363-12368	NN	denotes	mouse
T3416	12380-12385	NN	denotes	22C11
T3417	12369-12379	JJ	denotes	monoclonal
T3418	12386-12387	-LRB-	denotes	(
T3419	12442-12444	CD	denotes	17
T3420	12387-12391	JJ	denotes	kind
T3421	12392-12396	NN	denotes	gift
T3422	12397-12399	IN	denotes	of
T3423	12400-12406	NNP	denotes	Konrad
T3424	12407-12417	NNP	denotes	Beyreuther
T3425	12418-12421	CC	denotes	and
T3426	12422-12429	NNP	denotes	Andreas
T3427	12430-12439	NNP	denotes	Weidemann
T3428	12439-12440	:	denotes	;
T3429	12441-12442	-LRB-	denotes	[
T3430	12444-12445	-RRB-	denotes	]
T3431	12445-12446	-RRB-	denotes	)
T3432	12447-12454	VBN	denotes	diluted
T3433	12455-12456	CD	denotes	1
T3434	12456-12457	SYM	denotes	:
T3435	12457-12462	CD	denotes	1,000
T3436	12462-12464	,	denotes	,
T3437	12464-12469	NN	denotes	mouse
T3438	12481-12485	NN	denotes	6E10
T3439	12470-12480	JJ	denotes	monoclonal
T3440	12486-12487	-LRB-	denotes	(
T3441	12494-12506	NNP	denotes	Laboratories
T3442	12487-12493	NNP	denotes	Signet
T3443	12506-12508	,	denotes	,
T3444	12508-12514	NNP	denotes	Dedham
T3445	12514-12516	,	denotes	,
T3446	12516-12529	NNP	denotes	Massachusetts
T3447	12529-12531	,	denotes	,
T3448	12531-12537	NNP	denotes	United
T3449	12538-12544	NNP	denotes	States
T3450	12544-12545	-RRB-	denotes	)
T3451	12546-12553	VBN	denotes	diluted
T3452	12554-12555	CD	denotes	1
T3453	12555-12556	SYM	denotes	:
T3454	12556-12561	CD	denotes	2,500
T3455	12561-12563	,	denotes	,
T3456	12563-12569	NN	denotes	rabbit
T3457	12612-12617	NN	denotes	hSOD1
T3458	12570-12580	JJ	denotes	polyclonal
T3459	12581-12596	JJ	denotes	anti-superoxide
T3460	12597-12606	NN	denotes	dismutase
T3461	12607-12608	CD	denotes	1
T3462	12609-12610	-LRB-	denotes	(
T3463	12610-12611	NN	denotes	m
T3464	12611-12612	HYPH	denotes	/
T3465	12617-12618	-RRB-	denotes	)
T3466	12619-12620	-LRB-	denotes	[
T3467	12620-12622	CD	denotes	18
T3468	12622-12623	-RRB-	denotes	]
T3469	12624-12631	VBN	denotes	diluted
T3470	12632-12633	CD	denotes	1
T3471	12633-12634	SYM	denotes	:
T3472	12634-12639	CD	denotes	2,500
T3473	12640-12642	IN	denotes	to
T3474	12643-12644	CD	denotes	1
T3475	12644-12645	SYM	denotes	:
T3476	12645-12650	CD	denotes	4,000
T3477	12650-12652	,	denotes	,
T3478	12652-12654	CC	denotes	or
T3479	12655-12661	NN	denotes	rabbit
T3480	12673-12677	NN	denotes	CT15
T3481	12662-12672	JJ	denotes	polyclonal
T3482	12678-12679	-LRB-	denotes	(
T3483	12684-12688	NN	denotes	gift
T3484	12679-12683	JJ	denotes	kind
T3485	12689-12691	IN	denotes	of
T3486	12692-12694	NNP	denotes	Ed
T3487	12695-12698	NNP	denotes	Koo
T3488	12698-12699	:	denotes	;
T3489	12700-12701	-LRB-	denotes	[
T3490	12701-12703	CD	denotes	19
T3491	12703-12704	-RRB-	denotes	]
T3492	12704-12705	-RRB-	denotes	)
T3493	12706-12713	VBN	denotes	diluted
T3494	12714-12715	CD	denotes	1
T3495	12715-12716	SYM	denotes	:
T3496	12716-12721	CD	denotes	1,000
T3497	12721-12722	.	denotes	.
T3498	12722-12933	sentence	denotes	Subsequently, the blots were washed with PBS containing 0.1% Tween-20, and then incubated with either goat anti-mouse– or goat anti-rabbit–HRP conjugated secondary antibody diluted 1:1,000 in blocking solution.
T3499	12723-12735	RB	denotes	Subsequently
T3500	12752-12758	VBN	denotes	washed
T3501	12735-12737	,	denotes	,
T3502	12737-12740	DT	denotes	the
T3503	12741-12746	NNS	denotes	blots
T3504	12747-12751	VBD	denotes	were
T3505	12759-12763	IN	denotes	with
T3506	12764-12767	NN	denotes	PBS
T3507	12768-12778	VBG	denotes	containing
T3508	12779-12782	CD	denotes	0.1
T3509	12782-12783	NN	denotes	%
T3510	12784-12789	NN	denotes	Tween
T3511	12789-12790	HYPH	denotes	-
T3512	12790-12792	CD	denotes	20
T3513	12792-12794	,	denotes	,
T3514	12794-12797	CC	denotes	and
T3515	12798-12802	RB	denotes	then
T3516	12803-12812	VBN	denotes	incubated
T3517	12813-12817	IN	denotes	with
T3518	12818-12824	CC	denotes	either
T3519	12825-12829	NN	denotes	goat
T3520	12887-12895	NN	denotes	antibody
T3521	12830-12840	JJ	denotes	anti-mouse
T3522	12840-12841	HYPH	denotes	–
T3523	12842-12844	CC	denotes	or
T3524	12845-12849	NN	denotes	goat
T3525	12850-12861	JJ	denotes	anti-rabbit
T3526	12861-12862	HYPH	denotes	–
T3527	12862-12865	NN	denotes	HRP
T3528	12866-12876	VBN	denotes	conjugated
T3529	12877-12886	JJ	denotes	secondary
T3530	12896-12903	VBN	denotes	diluted
T3531	12904-12905	CD	denotes	1
T3532	12905-12906	SYM	denotes	:
T3533	12906-12911	CD	denotes	1,000
T3534	12912-12914	IN	denotes	in
T3535	12915-12923	NN	denotes	blocking
T3536	12924-12932	NN	denotes	solution
T3537	12932-12933	.	denotes	.
T3538	12933-13109	sentence	denotes	After several additional rinses in PBS with 0.1% Tween-20, blots were developed with enhanced chemiluminescence reagent and imaged with the Bio-Rad Molecular Imager FX system.
T3539	12934-12939	IN	denotes	After
T3540	13004-13013	VBN	denotes	developed
T3541	12940-12947	JJ	denotes	several
T3542	12959-12965	NNS	denotes	rinses
T3543	12948-12958	JJ	denotes	additional
T3544	12966-12968	IN	denotes	in
T3545	12969-12972	NN	denotes	PBS
T3546	12973-12977	IN	denotes	with
T3547	12978-12981	CD	denotes	0.1
T3548	12983-12988	NN	denotes	Tween
T3549	12981-12982	NN	denotes	%
T3550	12988-12989	HYPH	denotes	-
T3551	12989-12991	CD	denotes	20
T3552	12991-12993	,	denotes	,
T3553	12993-12998	NNS	denotes	blots
T3554	12999-13003	VBD	denotes	were
T3555	13014-13018	IN	denotes	with
T3556	13019-13027	VBN	denotes	enhanced
T3557	13046-13053	NN	denotes	reagent
T3558	13028-13045	NN	denotes	chemiluminescence
T3559	13054-13057	CC	denotes	and
T3560	13058-13064	VBN	denotes	imaged
T3561	13065-13069	IN	denotes	with
T3562	13070-13073	DT	denotes	the
T3563	13102-13108	NN	denotes	system
T3564	13074-13077	NNP	denotes	Bio
T3565	13078-13081	NNP	denotes	Rad
T3566	13077-13078	HYPH	denotes	-
T3567	13099-13101	NNP	denotes	FX
T3568	13082-13091	NNP	denotes	Molecular
T3569	13092-13098	NNP	denotes	Imager
T3570	13108-13109	.	denotes	.
T3571	13109-13252	sentence	denotes	Staining intensity within each lane was quantified using the Quantity One image analysis software (Molecular Imager FX, Bio-Rad Laboratories).
T3572	13110-13118	NN	denotes	Staining
T3573	13119-13128	NN	denotes	intensity
T3574	13150-13160	VBN	denotes	quantified
T3575	13129-13135	IN	denotes	within
T3576	13136-13140	DT	denotes	each
T3577	13141-13145	NN	denotes	lane
T3578	13146-13149	VBD	denotes	was
T3579	13161-13166	VBG	denotes	using
T3580	13167-13170	DT	denotes	the
T3581	13199-13207	NN	denotes	software
T3582	13171-13179	NNP	denotes	Quantity
T3583	13180-13183	CD	denotes	One
T3584	13184-13189	NN	denotes	image
T3585	13190-13198	NN	denotes	analysis
T3586	13208-13209	-LRB-	denotes	(
T3587	13226-13228	NNP	denotes	FX
T3588	13209-13218	NNP	denotes	Molecular
T3589	13219-13225	NNP	denotes	Imager
T3590	13228-13230	,	denotes	,
T3591	13230-13233	NNP	denotes	Bio
T3592	13234-13237	NNP	denotes	Rad
T3593	13233-13234	HYPH	denotes	-
T3594	13238-13250	NNP	denotes	Laboratories
T3595	13250-13251	-RRB-	denotes	)
T3596	13251-13252	.	denotes	.
T3597	13252-13337	sentence	denotes	Background was calculated from across the image and subtracted from the entire file.
T3598	13253-13263	NN	denotes	Background
T3599	13268-13278	VBN	denotes	calculated
T3600	13264-13267	VBD	denotes	was
T3601	13279-13283	IN	denotes	from
T3602	13284-13290	IN	denotes	across
T3603	13291-13294	DT	denotes	the
T3604	13295-13300	NN	denotes	image
T3605	13301-13304	CC	denotes	and
T3606	13305-13315	VBN	denotes	subtracted
T3607	13316-13320	IN	denotes	from
T3608	13321-13324	DT	denotes	the
T3609	13332-13336	NN	denotes	file
T3610	13325-13331	JJ	denotes	entire
T3611	13336-13337	.	denotes	.
T3612	13337-13466	sentence	denotes	The signal intensity for each band (corrected signal intensity × pixel number) was then calculated using the Volume report tool.
T3613	13338-13341	DT	denotes	The
T3614	13349-13358	NN	denotes	intensity
T3615	13342-13348	NN	denotes	signal
T3616	13426-13436	VBN	denotes	calculated
T3617	13359-13362	IN	denotes	for
T3618	13363-13367	DT	denotes	each
T3619	13368-13372	NN	denotes	band
T3620	13373-13374	-LRB-	denotes	(
T3621	13391-13400	NN	denotes	intensity
T3622	13374-13383	VBN	denotes	corrected
T3623	13384-13390	NN	denotes	signal
T3624	13401-13402	SYM	denotes	×
T3625	13403-13408	NN	denotes	pixel
T3626	13409-13415	NN	denotes	number
T3627	13415-13416	-RRB-	denotes	)
T3628	13417-13420	VBD	denotes	was
T3629	13421-13425	RB	denotes	then
T3630	13437-13442	VBG	denotes	using
T3631	13443-13446	DT	denotes	the
T3632	13461-13465	NN	denotes	tool
T3633	13447-13453	NN	denotes	Volume
T3634	13454-13460	NN	denotes	report
T3635	13465-13466	.	denotes	.
T3710	13468-13472	NN	denotes	Slot
T3711	13473-13477	NN	denotes	Blot
T3712	13483-13491	NN	denotes	Analysis
T3713	13478-13482	NN	denotes	mRNA
T3714	13491-13683	sentence	denotes	Five micrograms per sample of total RNA extracted from fresh-frozen brain, liver, kidney, heart, lung, spleen, and skeletal muscle was vacuum-filtered through 0.45-μm Optitran nitrocellulose.
T3715	13492-13496	CD	denotes	Five
T3716	13497-13507	NNS	denotes	micrograms
T3717	13634-13642	VBN	denotes	filtered
T3718	13508-13511	IN	denotes	per
T3719	13512-13518	NN	denotes	sample
T3720	13519-13521	IN	denotes	of
T3721	13522-13527	JJ	denotes	total
T3722	13528-13531	NN	denotes	RNA
T3723	13532-13541	VBN	denotes	extracted
T3724	13542-13546	IN	denotes	from
T3725	13547-13552	JJ	denotes	fresh
T3726	13553-13559	VBN	denotes	frozen
T3727	13552-13553	HYPH	denotes	-
T3728	13560-13565	NN	denotes	brain
T3729	13565-13567	,	denotes	,
T3730	13567-13572	NN	denotes	liver
T3731	13572-13574	,	denotes	,
T3732	13574-13580	NN	denotes	kidney
T3733	13580-13582	,	denotes	,
T3734	13582-13587	NN	denotes	heart
T3735	13587-13589	,	denotes	,
T3736	13589-13593	NN	denotes	lung
T3737	13593-13595	,	denotes	,
T3738	13595-13601	NN	denotes	spleen
T3739	13601-13603	,	denotes	,
T3740	13603-13606	CC	denotes	and
T3741	13607-13615	JJ	denotes	skeletal
T3742	13616-13622	NN	denotes	muscle
T3743	13623-13626	VBD	denotes	was
T3744	13627-13633	NN	denotes	vacuum
T3745	13633-13634	HYPH	denotes	-
T3746	13643-13650	IN	denotes	through
T3747	13651-13655	CD	denotes	0.45
T3748	13656-13658	NN	denotes	μm
T3749	13655-13656	HYPH	denotes	-
T3750	13668-13682	NN	denotes	nitrocellulose
T3751	13659-13667	NNP	denotes	Optitran
T3752	13682-13683	.	denotes	.
T3753	13683-13851	sentence	denotes	After several washes through the manifold with 10× SSC, blots were UV-cross-linked and probed with a radiolabeled ∼350-bp BglII–XhoI cDNA fragment of mo/huAPP695 cDNA.
T3754	13684-13689	IN	denotes	After
T3755	13754-13766	VBN	denotes	cross-linked
T3756	13690-13697	JJ	denotes	several
T3757	13698-13704	NNS	denotes	washes
T3758	13705-13712	IN	denotes	through
T3759	13713-13716	DT	denotes	the
T3760	13717-13725	NN	denotes	manifold
T3761	13726-13730	IN	denotes	with
T3762	13731-13733	CD	denotes	10
T3763	13735-13738	NN	denotes	SSC
T3764	13733-13734	SYM	denotes	×
T3765	13738-13740	,	denotes	,
T3766	13740-13745	NNS	denotes	blots
T3767	13746-13750	VBD	denotes	were
T3768	13751-13753	NN	denotes	UV
T3769	13753-13754	HYPH	denotes	-
T3770	13767-13770	CC	denotes	and
T3771	13771-13777	VBN	denotes	probed
T3772	13778-13782	IN	denotes	with
T3773	13783-13784	DT	denotes	a
T3774	13822-13830	NN	denotes	fragment
T3775	13785-13797	VBN	denotes	radiolabeled
T3776	13798-13799	SYM	denotes	∼
T3777	13799-13802	CD	denotes	350
T3778	13803-13805	NN	denotes	bp
T3779	13802-13803	HYPH	denotes	-
T3780	13806-13811	NN	denotes	BglII
T3781	13812-13816	NN	denotes	XhoI
T3782	13811-13812	HYPH	denotes	–
T3783	13817-13821	NN	denotes	cDNA
T3784	13831-13833	IN	denotes	of
T3785	13834-13836	NN	denotes	mo
T3786	13837-13845	NN	denotes	huAPP695
T3787	13836-13837	HYPH	denotes	/
T3788	13846-13850	NN	denotes	cDNA
T3789	13850-13851	.	denotes	.
T3790	13851-14183	sentence	denotes	After hybridizing overnight at 65 °C in 1% BSA/1 mM EDTA/0.5 M sodium phosphate buffer (pH 7.2)/7% SDS [20], the blots were washed twice at 65 °C for 30 min each in 0.1% BSA/1 mM EDTA/40 mM sodium phosphate buffer (pH 7.2)/5% SDS before two final 30-min washes at 65 °C with 1 mM EDTA/40 mM sodium phosphate buffer (pH 7.2)/1% SDS.
T3791	13852-13857	IN	denotes	After
T3792	13976-13982	VBN	denotes	washed
T3793	13858-13869	VBG	denotes	hybridizing
T3794	13870-13879	RB	denotes	overnight
T3795	13880-13882	IN	denotes	at
T3796	13883-13885	CD	denotes	65
T3797	13886-13888	NN	denotes	°C
T3798	13889-13891	IN	denotes	in
T3799	13892-13893	CD	denotes	1
T3800	13893-13894	NN	denotes	%
T3801	13895-13898	NN	denotes	BSA
T3802	13898-13899	HYPH	denotes	/
T3803	13899-13900	CD	denotes	1
T3804	13901-13903	NN	denotes	mM
T3805	13904-13908	NN	denotes	EDTA
T3806	13908-13909	HYPH	denotes	/
T3807	13909-13912	CD	denotes	0.5
T3808	13913-13914	NN	denotes	M
T3809	13932-13938	NN	denotes	buffer
T3810	13915-13921	NN	denotes	sodium
T3811	13922-13931	NN	denotes	phosphate
T3812	13939-13940	-LRB-	denotes	(
T3813	13940-13942	NN	denotes	pH
T3814	13943-13946	CD	denotes	7.2
T3815	13946-13947	-RRB-	denotes	)
T3816	13947-13948	HYPH	denotes	/
T3817	13948-13949	CD	denotes	7
T3818	13949-13950	NN	denotes	%
T3819	13951-13954	NN	denotes	SDS
T3820	13955-13956	-LRB-	denotes	[
T3821	13956-13958	CD	denotes	20
T3822	13958-13959	-RRB-	denotes	]
T3823	13959-13961	,	denotes	,
T3824	13961-13964	DT	denotes	the
T3825	13965-13970	NNS	denotes	blots
T3826	13971-13975	VBD	denotes	were
T3827	13983-13988	RB	denotes	twice
T3828	13989-13991	IN	denotes	at
T3829	13992-13994	CD	denotes	65
T3830	13995-13997	NN	denotes	°C
T3831	13998-14001	IN	denotes	for
T3832	14002-14004	CD	denotes	30
T3833	14005-14008	NN	denotes	min
T3834	14009-14013	DT	denotes	each
T3835	14014-14016	IN	denotes	in
T3836	14017-14020	CD	denotes	0.1
T3837	14020-14021	NN	denotes	%
T3838	14022-14025	NN	denotes	BSA
T3839	14025-14026	HYPH	denotes	/
T3840	14026-14027	CD	denotes	1
T3841	14028-14030	NN	denotes	mM
T3842	14031-14035	NN	denotes	EDTA
T3843	14035-14036	HYPH	denotes	/
T3844	14036-14038	CD	denotes	40
T3845	14039-14041	NN	denotes	mM
T3846	14059-14065	NN	denotes	buffer
T3847	14042-14048	NN	denotes	sodium
T3848	14049-14058	NN	denotes	phosphate
T3849	14066-14067	-LRB-	denotes	(
T3850	14067-14069	NN	denotes	pH
T3851	14070-14073	CD	denotes	7.2
T3852	14073-14074	-RRB-	denotes	)
T3853	14074-14075	HYPH	denotes	/
T3854	14075-14076	CD	denotes	5
T3855	14076-14077	NN	denotes	%
T3856	14078-14081	NN	denotes	SDS
T3857	14082-14088	IN	denotes	before
T3858	14089-14092	CD	denotes	two
T3859	14106-14112	NNS	denotes	washes
T3860	14093-14098	JJ	denotes	final
T3861	14099-14101	CD	denotes	30
T3862	14102-14105	NN	denotes	min
T3863	14101-14102	HYPH	denotes	-
T3864	14113-14115	IN	denotes	at
T3865	14116-14118	CD	denotes	65
T3866	14119-14121	NN	denotes	°C
T3867	14122-14126	IN	denotes	with
T3868	14127-14128	CD	denotes	1
T3869	14129-14131	NN	denotes	mM
T3870	14132-14136	NN	denotes	EDTA
T3871	14136-14137	HYPH	denotes	/
T3872	14137-14139	CD	denotes	40
T3873	14140-14142	NN	denotes	mM
T3874	14160-14166	NN	denotes	buffer
T3875	14143-14149	NN	denotes	sodium
T3876	14150-14159	NN	denotes	phosphate
T3877	14167-14168	-LRB-	denotes	(
T3878	14168-14170	NN	denotes	pH
T3879	14171-14174	CD	denotes	7.2
T3880	14174-14175	-RRB-	denotes	)
T3881	14175-14176	HYPH	denotes	/
T3882	14176-14177	CD	denotes	1
T3883	14177-14178	NN	denotes	%
T3884	14179-14182	NN	denotes	SDS
T3885	14182-14183	.	denotes	.
T3886	14183-14268	sentence	denotes	Blots were wrapped wet and exposed to phosphorscreens overnight at room temperature.
T3887	14184-14189	NNS	denotes	Blots
T3888	14195-14202	VBN	denotes	wrapped
T3889	14190-14194	VBD	denotes	were
T3890	14203-14206	JJ	denotes	wet
T3891	14207-14210	CC	denotes	and
T3892	14211-14218	VBN	denotes	exposed
T3893	14219-14221	IN	denotes	to
T3894	14222-14237	NNS	denotes	phosphorscreens
T3895	14238-14247	RB	denotes	overnight
T3896	14248-14250	IN	denotes	at
T3897	14251-14255	NN	denotes	room
T3898	14256-14267	NN	denotes	temperature
T3899	14267-14268	.	denotes	.
T4137	14270-14277	NN	denotes	Amyloid
T4138	14278-14287	NN	denotes	Histology
T4139	14287-14401	sentence	denotes	Mice were euthanized by ether inhalation and brains removed for immersion fixation in 4% paraformaldehyde/1× PBS.
T4140	14288-14292	NNS	denotes	Mice
T4141	14298-14308	VBN	denotes	euthanized
T4142	14293-14297	VBD	denotes	were
T4143	14309-14311	IN	denotes	by
T4144	14312-14317	NN	denotes	ether
T4145	14318-14328	NN	denotes	inhalation
T4146	14329-14332	CC	denotes	and
T4147	14333-14339	NNS	denotes	brains
T4148	14340-14347	VBN	denotes	removed
T4149	14348-14351	IN	denotes	for
T4150	14352-14361	NN	denotes	immersion
T4151	14362-14370	NN	denotes	fixation
T4152	14371-14373	IN	denotes	in
T4153	14374-14375	CD	denotes	4
T4154	14375-14376	NN	denotes	%
T4155	14377-14393	NN	denotes	paraformaldehyde
T4156	14393-14394	HYPH	denotes	/
T4157	14394-14395	CD	denotes	1
T4158	14397-14400	NN	denotes	PBS
T4159	14395-14396	SYM	denotes	×
T4160	14400-14401	.	denotes	.
T4161	14401-14579	sentence	denotes	After 48 h in fixative at 4 °C, brains were transferred to PBS, dehydrated in alcohols, treated with cedarwood oil and methylsalicylate, and embedded in paraffin for sectioning.
T4162	14402-14407	IN	denotes	After
T4163	14446-14457	VBN	denotes	transferred
T4164	14408-14410	CD	denotes	48
T4165	14411-14412	NN	denotes	h
T4166	14413-14415	IN	denotes	in
T4167	14416-14424	JJ	denotes	fixative
T4168	14425-14427	IN	denotes	at
T4169	14428-14429	CD	denotes	4
T4170	14430-14432	NN	denotes	°C
T4171	14432-14434	,	denotes	,
T4172	14434-14440	NNS	denotes	brains
T4173	14441-14445	VBD	denotes	were
T4174	14458-14460	IN	denotes	to
T4175	14461-14464	NN	denotes	PBS
T4176	14464-14466	,	denotes	,
T4177	14466-14476	VBN	denotes	dehydrated
T4178	14477-14479	IN	denotes	in
T4179	14480-14488	NNS	denotes	alcohols
T4180	14488-14490	,	denotes	,
T4181	14490-14497	VBN	denotes	treated
T4182	14498-14502	IN	denotes	with
T4183	14503-14512	NN	denotes	cedarwood
T4184	14513-14516	NN	denotes	oil
T4185	14517-14520	CC	denotes	and
T4186	14521-14537	NN	denotes	methylsalicylate
T4187	14537-14539	,	denotes	,
T4188	14539-14542	CC	denotes	and
T4189	14543-14551	VBN	denotes	embedded
T4190	14552-14554	IN	denotes	in
T4191	14555-14563	NN	denotes	paraffin
T4192	14564-14567	IN	denotes	for
T4193	14568-14578	VBG	denotes	sectioning
T4194	14578-14579	.	denotes	.
T4195	14579-14600	sentence	denotes	Hirano silver stain
T4196	14581-14587	NN	denotes	Hirano
T4197	14595-14600	NN	denotes	stain
T4198	14588-14594	NN	denotes	silver
T4199	14600-14738	sentence	denotes	Silver impregnation histology was performed on 10-μm paraffin-embedded sections by Hirano's modification of the Bielschowsky method [21].
T4200	14601-14607	NN	denotes	Silver
T4201	14621-14630	NN	denotes	histology
T4202	14608-14620	NN	denotes	impregnation
T4203	14635-14644	VBN	denotes	performed
T4204	14631-14634	VBD	denotes	was
T4205	14645-14647	IN	denotes	on
T4206	14648-14650	CD	denotes	10
T4207	14651-14653	NN	denotes	μm
T4208	14650-14651	HYPH	denotes	-
T4209	14672-14680	NNS	denotes	sections
T4210	14654-14662	NN	denotes	paraffin
T4211	14663-14671	VBN	denotes	embedded
T4212	14662-14663	HYPH	denotes	-
T4213	14681-14683	IN	denotes	by
T4214	14684-14690	NNP	denotes	Hirano
T4215	14693-14705	NN	denotes	modification
T4216	14690-14692	POS	denotes	's
T4217	14706-14708	IN	denotes	of
T4218	14709-14712	DT	denotes	the
T4219	14726-14732	NN	denotes	method
T4220	14713-14725	NNP	denotes	Bielschowsky
T4221	14733-14734	-LRB-	denotes	[
T4222	14734-14736	CD	denotes	21
T4223	14736-14737	-RRB-	denotes	]
T4224	14737-14738	.	denotes	.
T4225	14738-14890	sentence	denotes	Briefly, sections were deparaffinized through xylene and alcohols into tap water before being placed into fresh 20% silver nitrate solution for 20 min.
T4226	14739-14746	RB	denotes	Briefly
T4227	14762-14776	VBN	denotes	deparaffinized
T4228	14746-14748	,	denotes	,
T4229	14748-14756	NNS	denotes	sections
T4230	14757-14761	VBD	denotes	were
T4231	14777-14784	IN	denotes	through
T4232	14785-14791	NN	denotes	xylene
T4233	14792-14795	CC	denotes	and
T4234	14796-14804	NNS	denotes	alcohols
T4235	14805-14809	IN	denotes	into
T4236	14810-14813	NN	denotes	tap
T4237	14814-14819	NN	denotes	water
T4238	14820-14826	IN	denotes	before
T4239	14827-14832	VBG	denotes	being
T4240	14833-14839	VBN	denotes	placed
T4241	14840-14844	IN	denotes	into
T4242	14845-14850	JJ	denotes	fresh
T4243	14870-14878	NN	denotes	solution
T4244	14851-14853	CD	denotes	20
T4245	14853-14854	NN	denotes	%
T4246	14855-14861	NN	denotes	silver
T4247	14862-14869	NN	denotes	nitrate
T4248	14879-14882	IN	denotes	for
T4249	14883-14885	CD	denotes	20
T4250	14886-14889	NN	denotes	min
T4251	14889-14890	.	denotes	.
T4252	14890-15034	sentence	denotes	After being washed thoroughly with distilled water, slides were immersed in 20% silver nitrate solution titrated with fresh ammonium hydroxide.
T4253	14891-14896	IN	denotes	After
T4254	14955-14963	VBN	denotes	immersed
T4255	14897-14902	VBG	denotes	being
T4256	14903-14909	VBN	denotes	washed
T4257	14910-14920	RB	denotes	thoroughly
T4258	14921-14925	IN	denotes	with
T4259	14926-14935	JJ	denotes	distilled
T4260	14936-14941	NN	denotes	water
T4261	14941-14943	,	denotes	,
T4262	14943-14949	NNS	denotes	slides
T4263	14950-14954	VBD	denotes	were
T4264	14964-14966	IN	denotes	in
T4265	14967-14969	CD	denotes	20
T4266	14969-14970	NN	denotes	%
T4267	14986-14994	NN	denotes	solution
T4268	14971-14977	NN	denotes	silver
T4269	14978-14985	NN	denotes	nitrate
T4270	14995-15003	VBN	denotes	titrated
T4271	15004-15008	IN	denotes	with
T4272	15009-15014	JJ	denotes	fresh
T4273	15024-15033	NN	denotes	hydroxide
T4274	15015-15023	NN	denotes	ammonium
T4275	15033-15034	.	denotes	.
T4276	15034-15315	sentence	denotes	After 20 min, slides were washed with ammonia water before being individually developed with 100 μl of developer (20 ml of 37% formaldehyde, 100 ml of distilled water, 50 μl of concentrated nitric acid, and 0.5 g of citric acid) added to 50 ml of titrated silver nitrate solution.
T4277	15035-15040	IN	denotes	After
T4278	15061-15067	VBN	denotes	washed
T4279	15041-15043	CD	denotes	20
T4280	15044-15047	NN	denotes	min
T4281	15047-15049	,	denotes	,
T4282	15049-15055	NNS	denotes	slides
T4283	15056-15060	VBD	denotes	were
T4284	15068-15072	IN	denotes	with
T4285	15073-15080	NN	denotes	ammonia
T4286	15081-15086	NN	denotes	water
T4287	15087-15093	IN	denotes	before
T4288	15094-15099	VBG	denotes	being
T4289	15113-15122	VBN	denotes	developed
T4290	15100-15112	RB	denotes	individually
T4291	15123-15127	IN	denotes	with
T4292	15128-15131	CD	denotes	100
T4293	15132-15134	NN	denotes	μl
T4294	15135-15137	IN	denotes	of
T4295	15138-15147	NN	denotes	developer
T4296	15148-15149	-LRB-	denotes	(
T4297	15152-15154	NN	denotes	ml
T4298	15149-15151	CD	denotes	20
T4299	15155-15157	IN	denotes	of
T4300	15158-15160	CD	denotes	37
T4301	15160-15161	NN	denotes	%
T4302	15162-15174	NN	denotes	formaldehyde
T4303	15174-15176	,	denotes	,
T4304	15176-15179	CD	denotes	100
T4305	15180-15182	NN	denotes	ml
T4306	15183-15185	IN	denotes	of
T4307	15186-15195	JJ	denotes	distilled
T4308	15196-15201	NN	denotes	water
T4309	15201-15203	,	denotes	,
T4310	15203-15205	CD	denotes	50
T4311	15206-15208	NN	denotes	μl
T4312	15209-15211	IN	denotes	of
T4313	15212-15224	VBN	denotes	concentrated
T4314	15232-15236	NN	denotes	acid
T4315	15225-15231	JJ	denotes	nitric
T4316	15236-15238	,	denotes	,
T4317	15238-15241	CC	denotes	and
T4318	15242-15245	CD	denotes	0.5
T4319	15246-15247	NN	denotes	g
T4320	15248-15250	IN	denotes	of
T4321	15251-15257	JJ	denotes	citric
T4322	15258-15262	NN	denotes	acid
T4323	15262-15263	-RRB-	denotes	)
T4324	15264-15269	VBN	denotes	added
T4325	15270-15272	IN	denotes	to
T4326	15273-15275	CD	denotes	50
T4327	15276-15278	NN	denotes	ml
T4328	15279-15281	IN	denotes	of
T4329	15282-15290	VBN	denotes	titrated
T4330	15306-15314	NN	denotes	solution
T4331	15291-15297	NN	denotes	silver
T4332	15298-15305	NN	denotes	nitrate
T4333	15314-15315	.	denotes	.
T4334	15315-15429	sentence	denotes	Slides were then rinsed in tap water, fixed in 5% sodium thiosulfate, and dehydrated through alcohols and xylene.
T4335	15316-15322	NNS	denotes	Slides
T4336	15333-15339	VBN	denotes	rinsed
T4337	15323-15327	VBD	denotes	were
T4338	15328-15332	RB	denotes	then
T4339	15340-15342	IN	denotes	in
T4340	15343-15346	NN	denotes	tap
T4341	15347-15352	NN	denotes	water
T4342	15352-15354	,	denotes	,
T4343	15354-15359	VBN	denotes	fixed
T4344	15360-15362	IN	denotes	in
T4345	15363-15364	CD	denotes	5
T4346	15364-15365	NN	denotes	%
T4347	15373-15384	NN	denotes	thiosulfate
T4348	15366-15372	NN	denotes	sodium
T4349	15384-15386	,	denotes	,
T4350	15386-15389	CC	denotes	and
T4351	15390-15400	VBN	denotes	dehydrated
T4352	15401-15408	IN	denotes	through
T4353	15409-15417	NNS	denotes	alcohols
T4354	15418-15421	CC	denotes	and
T4355	15422-15428	NN	denotes	xylene
T4356	15428-15429	.	denotes	.
T4357	15429-15452	sentence	denotes	Thioflavin-S staining
T4358	15431-15441	NN	denotes	Thioflavin
T4359	15442-15443	NN	denotes	S
T4360	15441-15442	HYPH	denotes	-
T4361	15444-15452	NN	denotes	staining
T4362	15452-15638	sentence	denotes	Following deparaffinization of sections through xylene and alcohols, amyloid impregnation with thioflavin-S was performed according to the Guntern modification of the standard protocol.
T4363	15453-15462	VBG	denotes	Following
T4364	15565-15574	VBN	denotes	performed
T4365	15463-15480	NN	denotes	deparaffinization
T4366	15481-15483	IN	denotes	of
T4367	15484-15492	NNS	denotes	sections
T4368	15493-15500	IN	denotes	through
T4369	15501-15507	NN	denotes	xylene
T4370	15508-15511	CC	denotes	and
T4371	15512-15520	NNS	denotes	alcohols
T4372	15520-15522	,	denotes	,
T4373	15522-15529	NN	denotes	amyloid
T4374	15530-15542	NN	denotes	impregnation
T4375	15543-15547	IN	denotes	with
T4376	15548-15558	NN	denotes	thioflavin
T4377	15559-15560	NN	denotes	S
T4378	15558-15559	HYPH	denotes	-
T4379	15561-15564	VBD	denotes	was
T4380	15575-15584	VBG	denotes	according
T4381	15585-15587	IN	denotes	to
T4382	15588-15591	DT	denotes	the
T4383	15600-15612	NN	denotes	modification
T4384	15592-15599	NN	denotes	Guntern
T4385	15613-15615	IN	denotes	of
T4386	15616-15619	DT	denotes	the
T4387	15629-15637	NN	denotes	protocol
T4388	15620-15628	JJ	denotes	standard
T4389	15637-15638	.	denotes	.
T4390	15638-15858	sentence	denotes	Slides holding 10-μm paraffin sections were washed twice in distilled water, then immersed for 5 min in a 0.25% potassium permanganate solution, followed by 5 min in a 1% potassium metabisulfate/1% oxalic acid solution.
T4391	15639-15645	NNS	denotes	Slides
T4392	15683-15689	VBN	denotes	washed
T4393	15646-15653	VBG	denotes	holding
T4394	15654-15656	CD	denotes	10
T4395	15657-15659	NN	denotes	μm
T4396	15656-15657	HYPH	denotes	-
T4397	15669-15677	NNS	denotes	sections
T4398	15660-15668	NN	denotes	paraffin
T4399	15678-15682	VBD	denotes	were
T4400	15690-15695	RB	denotes	twice
T4401	15696-15698	IN	denotes	in
T4402	15699-15708	JJ	denotes	distilled
T4403	15709-15714	NN	denotes	water
T4404	15714-15716	,	denotes	,
T4405	15716-15720	RB	denotes	then
T4406	15721-15729	VBN	denotes	immersed
T4407	15730-15733	IN	denotes	for
T4408	15734-15735	CD	denotes	5
T4409	15736-15739	NN	denotes	min
T4410	15740-15742	IN	denotes	in
T4411	15743-15744	DT	denotes	a
T4412	15774-15782	NN	denotes	solution
T4413	15745-15749	CD	denotes	0.25
T4414	15749-15750	NN	denotes	%
T4415	15751-15760	NN	denotes	potassium
T4416	15761-15773	NN	denotes	permanganate
T4417	15782-15784	,	denotes	,
T4418	15784-15792	VBN	denotes	followed
T4419	15793-15795	IN	denotes	by
T4420	15796-15797	CD	denotes	5
T4421	15798-15801	NN	denotes	min
T4422	15802-15804	IN	denotes	in
T4423	15805-15806	DT	denotes	a
T4424	15849-15857	NN	denotes	solution
T4425	15807-15808	CD	denotes	1
T4426	15808-15809	NN	denotes	%
T4427	15820-15833	NN	denotes	metabisulfate
T4428	15810-15819	NN	denotes	potassium
T4429	15833-15834	HYPH	denotes	/
T4430	15834-15835	CD	denotes	1
T4431	15835-15836	NN	denotes	%
T4432	15844-15848	NN	denotes	acid
T4433	15837-15843	JJ	denotes	oxalic
T4434	15857-15858	.	denotes	.
T4435	15858-16010	sentence	denotes	After this preparation, slides were placed into a filtered aqueous 0.02% thioflavin-S solution (Chroma-Gesellschaft Schmid, Kongen, Germany) for 8 min.
T4436	15859-15864	IN	denotes	After
T4437	15895-15901	VBN	denotes	placed
T4438	15865-15869	DT	denotes	this
T4439	15870-15881	NN	denotes	preparation
T4440	15881-15883	,	denotes	,
T4441	15883-15889	NNS	denotes	slides
T4442	15890-15894	VBD	denotes	were
T4443	15902-15906	IN	denotes	into
T4444	15907-15908	DT	denotes	a
T4445	15945-15953	NN	denotes	solution
T4446	15909-15917	VBN	denotes	filtered
T4447	15918-15925	JJ	denotes	aqueous
T4448	15926-15930	CD	denotes	0.02
T4449	15930-15931	NN	denotes	%
T4450	15932-15942	NN	denotes	thioflavin
T4451	15943-15944	NN	denotes	S
T4452	15942-15943	HYPH	denotes	-
T4453	15954-15955	-LRB-	denotes	(
T4454	15975-15981	NNP	denotes	Schmid
T4455	15955-15961	NNP	denotes	Chroma
T4456	15962-15974	NNP	denotes	Gesellschaft
T4457	15961-15962	HYPH	denotes	-
T4458	15981-15983	,	denotes	,
T4459	15983-15989	NNP	denotes	Kongen
T4460	15989-15991	,	denotes	,
T4461	15991-15998	NNP	denotes	Germany
T4462	15998-15999	-RRB-	denotes	)
T4463	16000-16003	IN	denotes	for
T4464	16004-16005	CD	denotes	8
T4465	16006-16009	NN	denotes	min
T4466	16009-16010	.	denotes	.
T4467	16010-16194	sentence	denotes	Excess stain was removed by two brief rinses in 80% ethanol, then two in distilled water, after which slides were finished in aqueous mounting medium for florescence photomicrography.
T4468	16011-16017	JJ	denotes	Excess
T4469	16018-16023	NN	denotes	stain
T4470	16028-16035	VBN	denotes	removed
T4471	16024-16027	VBD	denotes	was
T4472	16036-16038	IN	denotes	by
T4473	16039-16042	CD	denotes	two
T4474	16049-16055	NNS	denotes	rinses
T4475	16043-16048	JJ	denotes	brief
T4476	16056-16058	IN	denotes	in
T4477	16059-16061	CD	denotes	80
T4478	16061-16062	NN	denotes	%
T4479	16063-16070	NN	denotes	ethanol
T4480	16070-16072	,	denotes	,
T4481	16072-16076	RB	denotes	then
T4482	16077-16080	CD	denotes	two
T4483	16081-16083	IN	denotes	in
T4484	16084-16093	JJ	denotes	distilled
T4485	16094-16099	NN	denotes	water
T4486	16099-16101	,	denotes	,
T4487	16101-16106	IN	denotes	after
T4488	16125-16133	VBN	denotes	finished
T4489	16107-16112	WDT	denotes	which
T4490	16113-16119	NNS	denotes	slides
T4491	16120-16124	VBD	denotes	were
T4492	16134-16136	IN	denotes	in
T4493	16137-16144	JJ	denotes	aqueous
T4494	16154-16160	NN	denotes	medium
T4495	16145-16153	NN	denotes	mounting
T4496	16161-16164	IN	denotes	for
T4497	16165-16176	NN	denotes	florescence
T4498	16177-16193	NN	denotes	photomicrography
T4499	16193-16194	.	denotes	.
T4500	16194-16267	sentence	denotes	Ubiquitin, glial fibrillary acidic protein, and Aβ immunohistochemistry
T4501	16196-16205	NN	denotes	Ubiquitin
T4502	16205-16207	,	denotes	,
T4503	16207-16212	JJ	denotes	glial
T4504	16231-16238	NN	denotes	protein
T4505	16213-16223	JJ	denotes	fibrillary
T4506	16224-16230	JJ	denotes	acidic
T4507	16238-16240	,	denotes	,
T4508	16240-16243	CC	denotes	and
T4509	16244-16246	NN	denotes	Aβ
T4510	16247-16267	NN	denotes	immunohistochemistry
T4511	16267-16368	sentence	denotes	Prior to immunostaining, slides were deparaffinized by oven heating followed by immersion in xylene.
T4512	16268-16273	JJ	denotes	Prior
T4513	16274-16276	IN	denotes	to
T4514	16305-16319	VBN	denotes	deparaffinized
T4515	16277-16291	NN	denotes	immunostaining
T4516	16291-16293	,	denotes	,
T4517	16293-16299	NNS	denotes	slides
T4518	16300-16304	VBD	denotes	were
T4519	16320-16322	IN	denotes	by
T4520	16323-16327	NN	denotes	oven
T4521	16328-16335	NN	denotes	heating
T4522	16336-16344	VBN	denotes	followed
T4523	16345-16347	IN	denotes	by
T4524	16348-16357	NN	denotes	immersion
T4525	16358-16360	IN	denotes	in
T4526	16361-16367	NN	denotes	xylene
T4527	16367-16368	.	denotes	.
T4528	16368-16523	sentence	denotes	After rehydration through graded alcohols into tap water, endogenous peroxidase activity was quenched by incubation with 3% hydrogen peroxide in methanol.
T4529	16369-16374	IN	denotes	After
T4530	16462-16470	VBN	denotes	quenched
T4531	16375-16386	NN	denotes	rehydration
T4532	16387-16394	IN	denotes	through
T4533	16395-16401	VBN	denotes	graded
T4534	16402-16410	NNS	denotes	alcohols
T4535	16411-16415	IN	denotes	into
T4536	16416-16419	NN	denotes	tap
T4537	16420-16425	NN	denotes	water
T4538	16425-16427	,	denotes	,
T4539	16427-16437	JJ	denotes	endogenous
T4540	16449-16457	NN	denotes	activity
T4541	16438-16448	NN	denotes	peroxidase
T4542	16458-16461	VBD	denotes	was
T4543	16471-16473	IN	denotes	by
T4544	16474-16484	NN	denotes	incubation
T4545	16485-16489	IN	denotes	with
T4546	16490-16491	CD	denotes	3
T4547	16491-16492	NN	denotes	%
T4548	16502-16510	NN	denotes	peroxide
T4549	16493-16501	NN	denotes	hydrogen
T4550	16511-16513	IN	denotes	in
T4551	16514-16522	NN	denotes	methanol
T4552	16522-16523	.	denotes	.
T4553	16523-16606	sentence	denotes	Slides were microwaved for 5–7 min in water, cooled for 5 min, then washed in TBS.
T4554	16524-16530	NNS	denotes	Slides
T4555	16536-16546	VBN	denotes	microwaved
T4556	16531-16535	VBD	denotes	were
T4557	16547-16550	IN	denotes	for
T4558	16551-16552	CD	denotes	5
T4559	16553-16554	CD	denotes	7
T4560	16552-16553	SYM	denotes	–
T4561	16555-16558	NN	denotes	min
T4562	16559-16561	IN	denotes	in
T4563	16562-16567	NN	denotes	water
T4564	16567-16569	,	denotes	,
T4565	16569-16575	VBN	denotes	cooled
T4566	16576-16579	IN	denotes	for
T4567	16580-16581	CD	denotes	5
T4568	16582-16585	NN	denotes	min
T4569	16585-16587	,	denotes	,
T4570	16587-16591	RB	denotes	then
T4571	16592-16598	VBN	denotes	washed
T4572	16599-16601	IN	denotes	in
T4573	16602-16605	NN	denotes	TBS
T4574	16605-16606	.	denotes	.
T4575	16606-16703	sentence	denotes	Nonspecific staining was blocked for 1 h with 3% normal goat serum and 0.1% Triton-X 100 in TBS.
T4576	16607-16618	JJ	denotes	Nonspecific
T4577	16619-16627	NN	denotes	staining
T4578	16632-16639	VBN	denotes	blocked
T4579	16628-16631	VBD	denotes	was
T4580	16640-16643	IN	denotes	for
T4581	16644-16645	CD	denotes	1
T4582	16646-16647	NN	denotes	h
T4583	16648-16652	IN	denotes	with
T4584	16653-16654	CD	denotes	3
T4585	16654-16655	NN	denotes	%
T4586	16668-16673	NN	denotes	serum
T4587	16656-16662	JJ	denotes	normal
T4588	16663-16667	NN	denotes	goat
T4589	16674-16677	CC	denotes	and
T4590	16678-16681	CD	denotes	0.1
T4591	16681-16682	NN	denotes	%
T4592	16690-16691	NN	denotes	X
T4593	16683-16689	NN	denotes	Triton
T4594	16689-16690	HYPH	denotes	-
T4595	16692-16695	CD	denotes	100
T4596	16696-16698	IN	denotes	in
T4597	16699-16702	NN	denotes	TBS
T4598	16702-16703	.	denotes	.
T4599	16703-17100	sentence	denotes	Slides were then placed into primary antibody (rabbit anti-Aβ peptide polyclonal antibody, Zymed Laboratories, South San Francisco, California, United States; rabbit anti-ubiquitin and rabbit anti–glial fibrillary acidic protein (GFAP) polyclonal antibodies, Dako, Carpinteria, California, United States) diluted 1:500 in TBS with 2% normal goat serum and incubated overnight at room temperature.
T4600	16704-16710	NNS	denotes	Slides
T4601	16721-16727	VBN	denotes	placed
T4602	16711-16715	VBD	denotes	were
T4603	16716-16720	RB	denotes	then
T4604	16728-16732	IN	denotes	into
T4605	16733-16740	JJ	denotes	primary
T4606	16741-16749	NN	denotes	antibody
T4607	16750-16751	-LRB-	denotes	(
T4608	16951-16961	NNS	denotes	antibodies
T4609	16751-16757	NN	denotes	rabbit
T4610	16785-16793	NN	denotes	antibody
T4611	16758-16765	JJ	denotes	anti-Aβ
T4612	16766-16773	NN	denotes	peptide
T4613	16774-16784	JJ	denotes	polyclonal
T4614	16793-16795	,	denotes	,
T4615	16795-16800	NNP	denotes	Zymed
T4616	16801-16813	NNP	denotes	Laboratories
T4617	16813-16815	,	denotes	,
T4618	16815-16820	NNP	denotes	South
T4619	16825-16834	NNP	denotes	Francisco
T4620	16821-16824	NNP	denotes	San
T4621	16834-16836	,	denotes	,
T4622	16836-16846	NNP	denotes	California
T4623	16846-16848	,	denotes	,
T4624	16848-16854	NNP	denotes	United
T4625	16855-16861	NNP	denotes	States
T4626	16861-16862	:	denotes	;
T4627	16863-16869	NN	denotes	rabbit
T4628	16870-16884	JJ	denotes	anti-ubiquitin
T4629	16885-16888	CC	denotes	and
T4630	16889-16895	NN	denotes	rabbit
T4631	16896-16906	JJ	denotes	anti–glial
T4632	16907-16917	JJ	denotes	fibrillary
T4633	16925-16932	NN	denotes	protein
T4634	16918-16924	JJ	denotes	acidic
T4635	16933-16934	-LRB-	denotes	(
T4636	16934-16938	NN	denotes	GFAP
T4637	16938-16939	-RRB-	denotes	)
T4638	16940-16950	JJ	denotes	polyclonal
T4639	16961-16963	,	denotes	,
T4640	16963-16967	NNP	denotes	Dako
T4641	16967-16969	,	denotes	,
T4642	16969-16980	NNP	denotes	Carpinteria
T4643	16980-16982	,	denotes	,
T4644	16982-16992	NNP	denotes	California
T4645	16992-16994	,	denotes	,
T4646	16994-17000	NNP	denotes	United
T4647	17001-17007	NNP	denotes	States
T4648	17007-17008	-RRB-	denotes	)
T4649	17009-17016	VBN	denotes	diluted
T4650	17017-17018	CD	denotes	1
T4651	17018-17019	SYM	denotes	:
T4652	17019-17022	CD	denotes	500
T4653	17023-17025	IN	denotes	in
T4654	17026-17029	NN	denotes	TBS
T4655	17030-17034	IN	denotes	with
T4656	17035-17036	CD	denotes	2
T4657	17036-17037	NN	denotes	%
T4658	17050-17055	NN	denotes	serum
T4659	17038-17044	JJ	denotes	normal
T4660	17045-17049	NN	denotes	goat
T4661	17056-17059	CC	denotes	and
T4662	17060-17069	VBN	denotes	incubated
T4663	17070-17079	RB	denotes	overnight
T4664	17080-17082	IN	denotes	at
T4665	17083-17087	NN	denotes	room
T4666	17088-17099	NN	denotes	temperature
T4667	17099-17100	.	denotes	.
T4668	17100-17507	sentence	denotes	After being washed of excess primary antibody with several changes of TBS, slides were incubated with either the Vectastain Elite anti-rabbit secondary system (anti-Aβ; Vector Laboratories, Burlingame, California, United States) or peroxidase/anti-peroxidase reagents (anti-ubitquitin and anti-GFAP; Sternberger Monoclonals, Lutherville, Maryland, United States) according to the manufacturers' directions.
T4669	17101-17106	IN	denotes	After
T4670	17188-17197	VBN	denotes	incubated
T4671	17107-17112	VBG	denotes	being
T4672	17113-17119	VBN	denotes	washed
T4673	17120-17122	IN	denotes	of
T4674	17123-17129	JJ	denotes	excess
T4675	17138-17146	NN	denotes	antibody
T4676	17130-17137	JJ	denotes	primary
T4677	17147-17151	IN	denotes	with
T4678	17152-17159	JJ	denotes	several
T4679	17160-17167	NNS	denotes	changes
T4680	17168-17170	IN	denotes	of
T4681	17171-17174	NN	denotes	TBS
T4682	17174-17176	,	denotes	,
T4683	17176-17182	NNS	denotes	slides
T4684	17183-17187	VBD	denotes	were
T4685	17198-17202	IN	denotes	with
T4686	17203-17209	CC	denotes	either
T4687	17253-17259	NN	denotes	system
T4688	17210-17213	DT	denotes	the
T4689	17214-17224	NNP	denotes	Vectastain
T4690	17225-17230	NNP	denotes	Elite
T4691	17231-17242	JJ	denotes	anti-rabbit
T4692	17243-17252	JJ	denotes	secondary
T4693	17260-17261	-LRB-	denotes	(
T4694	17277-17289	NNP	denotes	Laboratories
T4695	17261-17268	JJ	denotes	anti-Aβ
T4696	17268-17269	:	denotes	;
T4697	17270-17276	NNP	denotes	Vector
T4698	17289-17291	,	denotes	,
T4699	17291-17301	NNP	denotes	Burlingame
T4700	17301-17303	,	denotes	,
T4701	17303-17313	NNP	denotes	California
T4702	17313-17315	,	denotes	,
T4703	17315-17321	NNP	denotes	United
T4704	17322-17328	NNP	denotes	States
T4705	17328-17329	-RRB-	denotes	)
T4706	17330-17332	CC	denotes	or
T4707	17333-17343	NN	denotes	peroxidase
T4708	17360-17368	NNS	denotes	reagents
T4709	17343-17344	HYPH	denotes	/
T4710	17344-17359	JJ	denotes	anti-peroxidase
T4711	17369-17370	-LRB-	denotes	(
T4712	17413-17424	NNPS	denotes	Monoclonals
T4713	17370-17385	JJ	denotes	anti-ubitquitin
T4714	17386-17389	CC	denotes	and
T4715	17390-17399	JJ	denotes	anti-GFAP
T4716	17399-17400	:	denotes	;
T4717	17401-17412	NNP	denotes	Sternberger
T4718	17424-17426	,	denotes	,
T4719	17426-17437	NNP	denotes	Lutherville
T4720	17437-17439	,	denotes	,
T4721	17439-17447	NNP	denotes	Maryland
T4722	17447-17449	,	denotes	,
T4723	17449-17455	NNP	denotes	United
T4724	17456-17462	NNP	denotes	States
T4725	17462-17463	-RRB-	denotes	)
T4726	17464-17473	VBG	denotes	according
T4727	17474-17476	IN	denotes	to
T4728	17477-17480	DT	denotes	the
T4729	17481-17494	NNS	denotes	manufacturers
T4730	17496-17506	NNS	denotes	directions
T4731	17494-17495	POS	denotes	'
T4732	17506-17507	.	denotes	.
T4733	17507-17613	sentence	denotes	Antibody binding was visualized with diaminobenzidene, and sections were counterstained with hematoxylin.
T4734	17508-17516	NN	denotes	Antibody
T4735	17517-17524	NN	denotes	binding
T4736	17529-17539	VBN	denotes	visualized
T4737	17525-17528	VBD	denotes	was
T4738	17540-17544	IN	denotes	with
T4739	17545-17561	NN	denotes	diaminobenzidene
T4740	17561-17563	,	denotes	,
T4741	17563-17566	CC	denotes	and
T4742	17567-17575	NNS	denotes	sections
T4743	17581-17595	VBN	denotes	counterstained
T4744	17576-17580	VBD	denotes	were
T4745	17596-17600	IN	denotes	with
T4746	17601-17612	NN	denotes	hematoxylin
T4747	17612-17613	.	denotes	.
T4787	17615-17621	NN	denotes	Filter
T4788	17627-17632	NN	denotes	Assay
T4789	17622-17626	NN	denotes	Trap
T4790	17632-17784	sentence	denotes	An aliquot of each cortical homogenate used for Western blotting above was partially solubilized by the addition of SDS to a final concentration of 1%.
T4791	17633-17635	DT	denotes	An
T4792	17636-17643	NN	denotes	aliquot
T4793	17718-17729	VBN	denotes	solubilized
T4794	17644-17646	IN	denotes	of
T4795	17647-17651	DT	denotes	each
T4796	17661-17671	NN	denotes	homogenate
T4797	17652-17660	JJ	denotes	cortical
T4798	17672-17676	VBN	denotes	used
T4799	17677-17680	IN	denotes	for
T4800	17681-17688	NNP	denotes	Western
T4801	17689-17697	NN	denotes	blotting
T4802	17698-17703	RB	denotes	above
T4803	17704-17707	VBD	denotes	was
T4804	17708-17717	RB	denotes	partially
T4805	17730-17732	IN	denotes	by
T4806	17733-17736	DT	denotes	the
T4807	17737-17745	NN	denotes	addition
T4808	17746-17748	IN	denotes	of
T4809	17749-17752	NN	denotes	SDS
T4810	17753-17755	IN	denotes	to
T4811	17756-17757	DT	denotes	a
T4812	17764-17777	NN	denotes	concentration
T4813	17758-17763	JJ	denotes	final
T4814	17778-17780	IN	denotes	of
T4815	17781-17782	CD	denotes	1
T4816	17782-17783	NN	denotes	%
T4817	17783-17784	.	denotes	.
T4818	17784-17972	sentence	denotes	Serial 1:2 dilutions were made with 1× PBS/1% SDS, and 100 μl of each dilution was then vacuum-filtered through a pre-wet 0.22-μm cellulose acetate membrane (Schleicher and Schuell) [22].
T4819	17785-17791	JJ	denotes	Serial
T4820	17796-17805	NNS	denotes	dilutions
T4821	17792-17793	CD	denotes	1
T4822	17793-17794	SYM	denotes	:
T4823	17794-17795	CD	denotes	2
T4824	17811-17815	VBN	denotes	made
T4825	17806-17810	VBD	denotes	were
T4826	17816-17820	IN	denotes	with
T4827	17821-17822	CD	denotes	1
T4828	17824-17827	NN	denotes	PBS
T4829	17822-17823	SYM	denotes	×
T4830	17827-17828	HYPH	denotes	/
T4831	17828-17829	CD	denotes	1
T4832	17829-17830	NN	denotes	%
T4833	17831-17834	NN	denotes	SDS
T4834	17834-17836	,	denotes	,
T4835	17836-17839	CC	denotes	and
T4836	17840-17843	CD	denotes	100
T4837	17844-17846	NN	denotes	μl
T4838	17880-17888	VBN	denotes	filtered
T4839	17847-17849	IN	denotes	of
T4840	17850-17854	DT	denotes	each
T4841	17855-17863	NN	denotes	dilution
T4842	17864-17867	VBD	denotes	was
T4843	17868-17872	RB	denotes	then
T4844	17873-17879	NN	denotes	vacuum
T4845	17879-17880	HYPH	denotes	-
T4846	17889-17896	IN	denotes	through
T4847	17897-17898	DT	denotes	a
T4848	17933-17941	NN	denotes	membrane
T4849	17899-17906	JJ	denotes	pre-wet
T4850	17907-17911	CD	denotes	0.22
T4851	17912-17914	NN	denotes	μm
T4852	17911-17912	HYPH	denotes	-
T4853	17915-17924	NN	denotes	cellulose
T4854	17925-17932	NN	denotes	acetate
T4855	17942-17943	-LRB-	denotes	(
T4856	17943-17953	NNP	denotes	Schleicher
T4857	17954-17957	CC	denotes	and
T4858	17958-17965	NNP	denotes	Schuell
T4859	17965-17966	-RRB-	denotes	)
T4860	17967-17968	-LRB-	denotes	[
T4861	17968-17970	CD	denotes	22
T4862	17970-17971	-RRB-	denotes	]
T4863	17971-17972	.	denotes	.
T4864	17972-18188	sentence	denotes	Each well was washed several times with PBS, after which blots were incubated overnight with polyclonal anti-Aβ antibody (Zymed Laboratories) diluted 1:600 in a blocking solution of 1× TBS/5% nonfat dry milk powder.
T4865	17973-17977	DT	denotes	Each
T4866	17978-17982	NN	denotes	well
T4867	17987-17993	VBN	denotes	washed
T4868	17983-17986	VBD	denotes	was
T4869	17994-18001	JJ	denotes	several
T4870	18002-18007	NNS	denotes	times
T4871	18008-18012	IN	denotes	with
T4872	18013-18016	NN	denotes	PBS
T4873	18016-18018	,	denotes	,
T4874	18018-18023	IN	denotes	after
T4875	18041-18050	VBN	denotes	incubated
T4876	18024-18029	WDT	denotes	which
T4877	18030-18035	NNS	denotes	blots
T4878	18036-18040	VBD	denotes	were
T4879	18051-18060	RB	denotes	overnight
T4880	18061-18065	IN	denotes	with
T4881	18066-18076	JJ	denotes	polyclonal
T4882	18085-18093	NN	denotes	antibody
T4883	18077-18084	JJ	denotes	anti-Aβ
T4884	18094-18095	-LRB-	denotes	(
T4885	18101-18113	NNP	denotes	Laboratories
T4886	18095-18100	NNP	denotes	Zymed
T4887	18113-18114	-RRB-	denotes	)
T4888	18115-18122	VBN	denotes	diluted
T4889	18123-18124	CD	denotes	1
T4890	18124-18125	SYM	denotes	:
T4891	18125-18128	CD	denotes	600
T4892	18129-18131	IN	denotes	in
T4893	18132-18133	DT	denotes	a
T4894	18143-18151	NN	denotes	solution
T4895	18134-18142	NN	denotes	blocking
T4896	18152-18154	IN	denotes	of
T4897	18155-18156	CD	denotes	1
T4898	18158-18161	NN	denotes	TBS
T4899	18156-18157	SYM	denotes	×
T4900	18181-18187	NN	denotes	powder
T4901	18161-18162	HYPH	denotes	/
T4902	18162-18163	CD	denotes	5
T4903	18163-18164	NN	denotes	%
T4904	18176-18180	NN	denotes	milk
T4905	18165-18171	JJ	denotes	nonfat
T4906	18172-18175	JJ	denotes	dry
T4907	18187-18188	.	denotes	.
T4908	18188-18376	sentence	denotes	After washing the blots three times for 10 min each in 1× TBS/0.1% Tween-20, the membrane was incubated for 1 h with HRP-conjugated protein A (Sigma) diluted 1:5,000 in blocking solution.
T4909	18189-18194	IN	denotes	After
T4910	18283-18292	VBN	denotes	incubated
T4911	18195-18202	VBG	denotes	washing
T4912	18203-18206	DT	denotes	the
T4913	18207-18212	NNS	denotes	blots
T4914	18213-18218	CD	denotes	three
T4915	18219-18224	NNS	denotes	times
T4916	18225-18228	IN	denotes	for
T4917	18229-18231	CD	denotes	10
T4918	18232-18235	NN	denotes	min
T4919	18236-18240	DT	denotes	each
T4920	18241-18243	IN	denotes	in
T4921	18244-18245	CD	denotes	1
T4922	18247-18250	NN	denotes	TBS
T4923	18245-18246	SYM	denotes	×
T4924	18250-18251	HYPH	denotes	/
T4925	18251-18254	CD	denotes	0.1
T4926	18254-18255	NN	denotes	%
T4927	18256-18261	NN	denotes	Tween
T4928	18261-18262	HYPH	denotes	-
T4929	18262-18264	CD	denotes	20
T4930	18264-18266	,	denotes	,
T4931	18266-18269	DT	denotes	the
T4932	18270-18278	NN	denotes	membrane
T4933	18279-18282	VBD	denotes	was
T4934	18293-18296	IN	denotes	for
T4935	18297-18298	CD	denotes	1
T4936	18299-18300	NN	denotes	h
T4937	18301-18305	IN	denotes	with
T4938	18306-18309	NN	denotes	HRP
T4939	18310-18320	VBN	denotes	conjugated
T4940	18309-18310	HYPH	denotes	-
T4941	18329-18330	NN	denotes	A
T4942	18321-18328	NN	denotes	protein
T4943	18331-18332	-LRB-	denotes	(
T4944	18332-18337	NNP	denotes	Sigma
T4945	18337-18338	-RRB-	denotes	)
T4946	18339-18346	VBN	denotes	diluted
T4947	18347-18348	CD	denotes	1
T4948	18348-18349	SYM	denotes	:
T4949	18349-18354	CD	denotes	5,000
T4950	18355-18357	IN	denotes	in
T4951	18358-18366	NN	denotes	blocking
T4952	18367-18375	NN	denotes	solution
T4953	18375-18376	.	denotes	.
T4954	18376-18569	sentence	denotes	The membranes were again washed three times with 1× TBS/0.1% Tween-20, before antibody binding was detected with enhanced chemiluminescence (PerkinElmer, Boston, Massachusetts, United States).
T4955	18377-18380	DT	denotes	The
T4956	18381-18390	NNS	denotes	membranes
T4957	18402-18408	VBN	denotes	washed
T4958	18391-18395	VBD	denotes	were
T4959	18396-18401	RB	denotes	again
T4960	18409-18414	CD	denotes	three
T4961	18415-18420	NNS	denotes	times
T4962	18421-18425	IN	denotes	with
T4963	18426-18427	CD	denotes	1
T4964	18429-18432	NN	denotes	TBS
T4965	18427-18428	SYM	denotes	×
T4966	18432-18433	HYPH	denotes	/
T4967	18433-18436	CD	denotes	0.1
T4968	18436-18437	NN	denotes	%
T4969	18438-18443	NN	denotes	Tween
T4970	18443-18444	HYPH	denotes	-
T4971	18444-18446	CD	denotes	20
T4972	18446-18448	,	denotes	,
T4973	18448-18454	IN	denotes	before
T4974	18476-18484	VBN	denotes	detected
T4975	18455-18463	NN	denotes	antibody
T4976	18464-18471	NN	denotes	binding
T4977	18472-18475	VBD	denotes	was
T4978	18485-18489	IN	denotes	with
T4979	18490-18498	VBN	denotes	enhanced
T4980	18499-18516	NN	denotes	chemiluminescence
T4981	18517-18518	-LRB-	denotes	(
T4982	18518-18529	NNP	denotes	PerkinElmer
T4983	18529-18531	,	denotes	,
T4984	18531-18537	NNP	denotes	Boston
T4985	18537-18539	,	denotes	,
T4986	18539-18552	NNP	denotes	Massachusetts
T4987	18552-18554	,	denotes	,
T4988	18554-18560	NNP	denotes	United
T4989	18561-18567	NNP	denotes	States
T4990	18567-18568	-RRB-	denotes	)
T4991	18568-18569	.	denotes	.
T4992	18569-18756	sentence	denotes	Digital images of each blot were captured with a Molecular Imager FX gel documentation system, and the intensity of Aβ staining was quantified using Quantity One image analysis software.
T4993	18570-18577	JJ	denotes	Digital
T4994	18578-18584	NNS	denotes	images
T4995	18603-18611	VBN	denotes	captured
T4996	18585-18587	IN	denotes	of
T4997	18588-18592	DT	denotes	each
T4998	18593-18597	NN	denotes	blot
T4999	18598-18602	VBD	denotes	were
T5000	18612-18616	IN	denotes	with
T5001	18617-18618	DT	denotes	a
T5002	18657-18663	NN	denotes	system
T5003	18619-18628	JJ	denotes	Molecular
T5004	18629-18635	NNP	denotes	Imager
T5005	18636-18638	NNP	denotes	FX
T5006	18639-18642	NN	denotes	gel
T5007	18643-18656	NN	denotes	documentation
T5008	18663-18665	,	denotes	,
T5009	18665-18668	CC	denotes	and
T5010	18669-18672	DT	denotes	the
T5011	18673-18682	NN	denotes	intensity
T5012	18702-18712	VBN	denotes	quantified
T5013	18683-18685	IN	denotes	of
T5014	18686-18688	NN	denotes	Aβ
T5015	18689-18697	NN	denotes	staining
T5016	18698-18701	VBD	denotes	was
T5017	18713-18718	VBG	denotes	using
T5018	18719-18727	NNP	denotes	Quantity
T5019	18747-18755	NN	denotes	software
T5020	18728-18731	CD	denotes	One
T5021	18732-18737	NN	denotes	image
T5022	18738-18746	NN	denotes	analysis
T5023	18755-18756	.	denotes	.
T5075	18758-18760	NN	denotes	Aβ
T5076	18761-18766	NN	denotes	ELISA
T5077	18766-18944	sentence	denotes	An aliquot of cortical homogenate generated for Western analysis described above was subjected to a three-step sequential extraction using PBS, 2% SDS, and 70% formic acid (FA).
T5078	18767-18769	DT	denotes	An
T5079	18770-18777	NN	denotes	aliquot
T5080	18852-18861	VBN	denotes	subjected
T5081	18778-18780	IN	denotes	of
T5082	18781-18789	JJ	denotes	cortical
T5083	18790-18800	NN	denotes	homogenate
T5084	18801-18810	VBN	denotes	generated
T5085	18811-18814	IN	denotes	for
T5086	18815-18822	NNP	denotes	Western
T5087	18823-18831	NN	denotes	analysis
T5088	18832-18841	VBN	denotes	described
T5089	18842-18847	RB	denotes	above
T5090	18848-18851	VBD	denotes	was
T5091	18862-18864	IN	denotes	to
T5092	18865-18866	DT	denotes	a
T5093	18889-18899	NN	denotes	extraction
T5094	18867-18872	CD	denotes	three
T5095	18873-18877	NN	denotes	step
T5096	18872-18873	HYPH	denotes	-
T5097	18878-18888	JJ	denotes	sequential
T5098	18900-18905	VBG	denotes	using
T5099	18906-18909	NN	denotes	PBS
T5100	18909-18911	,	denotes	,
T5101	18911-18912	CD	denotes	2
T5102	18912-18913	NN	denotes	%
T5103	18914-18917	NN	denotes	SDS
T5104	18917-18919	,	denotes	,
T5105	18919-18922	CC	denotes	and
T5106	18923-18925	CD	denotes	70
T5107	18925-18926	NN	denotes	%
T5108	18927-18933	JJ	denotes	formic
T5109	18934-18938	NN	denotes	acid
T5110	18939-18940	-LRB-	denotes	(
T5111	18940-18942	NN	denotes	FA
T5112	18942-18943	-RRB-	denotes	)
T5113	18943-18944	.	denotes	.
T5114	18944-19140	sentence	denotes	At each step, the sample was sonicated in appropriate buffer and centrifuged at 100,000g for 30 min (1- to 1.5-mo samples) or 60 min (6- to 12-mo samples) at 4 °C as previously described [23–25].
T5115	18945-18947	IN	denotes	At
T5116	18974-18983	VBN	denotes	sonicated
T5117	18948-18952	DT	denotes	each
T5118	18953-18957	NN	denotes	step
T5119	18957-18959	,	denotes	,
T5120	18959-18962	DT	denotes	the
T5121	18963-18969	NN	denotes	sample
T5122	18970-18973	VBD	denotes	was
T5123	18984-18986	IN	denotes	in
T5124	18987-18998	JJ	denotes	appropriate
T5125	18999-19005	NN	denotes	buffer
T5126	19006-19009	CC	denotes	and
T5127	19010-19021	VBN	denotes	centrifuged
T5128	19022-19024	IN	denotes	at
T5129	19025-19033	CD	denotes	100,000g
T5130	19034-19037	IN	denotes	for
T5131	19038-19040	CD	denotes	30
T5132	19041-19044	NN	denotes	min
T5133	19045-19046	-LRB-	denotes	(
T5134	19059-19066	NNS	denotes	samples
T5135	19046-19047	CD	denotes	1
T5136	19052-19055	CD	denotes	1.5
T5137	19047-19048	HYPH	denotes	-
T5138	19049-19051	IN	denotes	to
T5139	19056-19058	NN	denotes	mo
T5140	19055-19056	HYPH	denotes	-
T5141	19066-19067	-RRB-	denotes	)
T5142	19068-19070	CC	denotes	or
T5143	19071-19073	CD	denotes	60
T5144	19074-19077	NN	denotes	min
T5145	19078-19079	-LRB-	denotes	(
T5146	19091-19098	NNS	denotes	samples
T5147	19079-19080	CD	denotes	6
T5148	19085-19087	CD	denotes	12
T5149	19080-19081	HYPH	denotes	-
T5150	19082-19084	IN	denotes	to
T5151	19088-19090	NN	denotes	mo
T5152	19087-19088	HYPH	denotes	-
T5153	19098-19099	-RRB-	denotes	)
T5154	19100-19102	IN	denotes	at
T5155	19103-19104	CD	denotes	4
T5156	19105-19107	NN	denotes	°C
T5157	19108-19110	IN	denotes	as
T5158	19122-19131	VBN	denotes	described
T5159	19111-19121	RB	denotes	previously
T5160	19132-19133	-LRB-	denotes	[
T5161	19133-19135	CD	denotes	23
T5162	19135-19136	SYM	denotes	–
T5163	19136-19138	CD	denotes	25
T5164	19138-19139	-RRB-	denotes	]
T5165	19139-19140	.	denotes	.
T5166	19140-19245	sentence	denotes	The supernatant was removed for analysis, and the pellet was sonicated in the next solution in sequence.
T5167	19141-19144	DT	denotes	The
T5168	19145-19156	NN	denotes	supernatant
T5169	19161-19168	VBN	denotes	removed
T5170	19157-19160	VBD	denotes	was
T5171	19169-19172	IN	denotes	for
T5172	19173-19181	NN	denotes	analysis
T5173	19181-19183	,	denotes	,
T5174	19183-19186	CC	denotes	and
T5175	19187-19190	DT	denotes	the
T5176	19191-19197	NN	denotes	pellet
T5177	19202-19211	VBN	denotes	sonicated
T5178	19198-19201	VBD	denotes	was
T5179	19212-19214	IN	denotes	in
T5180	19215-19218	DT	denotes	the
T5181	19224-19232	NN	denotes	solution
T5182	19219-19223	JJ	denotes	next
T5183	19233-19235	IN	denotes	in
T5184	19236-19244	NN	denotes	sequence
T5185	19244-19245	.	denotes	.
T5186	19245-19408	sentence	denotes	The 2% SDS extracts were diluted in EC buffer, and the FA extracts neutralized with 1M Tris-phosphate buffer (pH 11) then diluted with EC buffer prior to testing.
T5187	19246-19249	DT	denotes	The
T5188	19257-19265	NNS	denotes	extracts
T5189	19250-19251	CD	denotes	2
T5190	19251-19252	NN	denotes	%
T5191	19253-19256	NN	denotes	SDS
T5192	19271-19278	VBN	denotes	diluted
T5193	19266-19270	VBD	denotes	were
T5194	19279-19281	IN	denotes	in
T5195	19282-19284	NN	denotes	EC
T5196	19285-19291	NN	denotes	buffer
T5197	19291-19293	,	denotes	,
T5198	19293-19296	CC	denotes	and
T5199	19297-19300	DT	denotes	the
T5200	19304-19312	NNS	denotes	extracts
T5201	19301-19303	NN	denotes	FA
T5202	19313-19324	VBN	denotes	neutralized
T5203	19325-19329	IN	denotes	with
T5204	19330-19332	NN	denotes	1M
T5205	19348-19354	NN	denotes	buffer
T5206	19333-19337	NN	denotes	Tris
T5207	19337-19338	HYPH	denotes	-
T5208	19338-19347	NN	denotes	phosphate
T5209	19355-19356	-LRB-	denotes	(
T5210	19356-19358	NN	denotes	pH
T5211	19359-19361	CD	denotes	11
T5212	19361-19362	-RRB-	denotes	)
T5213	19363-19367	RB	denotes	then
T5214	19368-19375	VBD	denotes	diluted
T5215	19376-19380	IN	denotes	with
T5216	19381-19383	NN	denotes	EC
T5217	19384-19390	NN	denotes	buffer
T5218	19391-19396	JJ	denotes	prior
T5219	19397-19399	IN	denotes	to
T5220	19400-19407	NN	denotes	testing
T5221	19407-19408	.	denotes	.
T5222	19408-19594	sentence	denotes	Human Aβ was measured in each fraction using BAN50 for capture (epitope Aβ1–16) and BA27 and BC05 for detection (Aβ40 and Aβ42, respectively) (Takeda Chemical Industries, Osaka, Japan).
T5223	19409-19414	JJ	denotes	Human
T5224	19415-19417	NN	denotes	Aβ
T5225	19422-19430	VBN	denotes	measured
T5226	19418-19421	VBD	denotes	was
T5227	19431-19433	IN	denotes	in
T5228	19434-19438	DT	denotes	each
T5229	19439-19447	NN	denotes	fraction
T5230	19448-19453	VBG	denotes	using
T5231	19454-19459	NN	denotes	BAN50
T5232	19460-19463	IN	denotes	for
T5233	19464-19471	NN	denotes	capture
T5234	19472-19473	-LRB-	denotes	(
T5235	19485-19487	NN	denotes	16
T5236	19473-19480	NN	denotes	epitope
T5237	19481-19484	NN	denotes	Aβ1
T5238	19484-19485	HYPH	denotes	–
T5239	19487-19488	-RRB-	denotes	)
T5240	19489-19492	CC	denotes	and
T5241	19493-19497	NN	denotes	BA27
T5242	19498-19501	CC	denotes	and
T5243	19502-19506	NN	denotes	BC05
T5244	19507-19510	IN	denotes	for
T5245	19511-19520	NN	denotes	detection
T5246	19521-19522	-LRB-	denotes	(
T5247	19522-19526	NN	denotes	Aβ40
T5248	19527-19530	CC	denotes	and
T5249	19531-19535	NN	denotes	Aβ42
T5250	19535-19537	,	denotes	,
T5251	19537-19549	RB	denotes	respectively
T5252	19549-19550	-RRB-	denotes	)
T5253	19551-19552	-LRB-	denotes	(
T5254	19568-19578	NNPS	denotes	Industries
T5255	19552-19558	NNP	denotes	Takeda
T5256	19559-19567	NNP	denotes	Chemical
T5257	19578-19580	,	denotes	,
T5258	19580-19585	NNP	denotes	Osaka
T5259	19585-19587	,	denotes	,
T5260	19587-19592	NNP	denotes	Japan
T5261	19592-19593	-RRB-	denotes	)
T5262	19593-19594	.	denotes	.
T5263	19594-19751	sentence	denotes	Total Aβ (mouse + human; 1- to 1.5-mo samples only) was measured in each fraction using BNT77 for capture (epitope Aβ11–28) and BA27 and BC05 for detection.
T5264	19595-19600	JJ	denotes	Total
T5265	19601-19603	NN	denotes	Aβ
T5266	19651-19659	VBN	denotes	measured
T5267	19604-19605	-LRB-	denotes	(
T5268	19633-19640	NNS	denotes	samples
T5269	19605-19610	NN	denotes	mouse
T5270	19611-19612	SYM	denotes	+
T5271	19613-19618	JJ	denotes	human
T5272	19618-19619	:	denotes	;
T5273	19620-19621	CD	denotes	1
T5274	19626-19629	CD	denotes	1.5
T5275	19621-19622	HYPH	denotes	-
T5276	19623-19625	IN	denotes	to
T5277	19630-19632	NN	denotes	mo
T5278	19629-19630	HYPH	denotes	-
T5279	19641-19645	RB	denotes	only
T5280	19645-19646	-RRB-	denotes	)
T5281	19647-19650	VBD	denotes	was
T5282	19660-19662	IN	denotes	in
T5283	19663-19667	DT	denotes	each
T5284	19668-19676	NN	denotes	fraction
T5285	19677-19682	VBG	denotes	using
T5286	19683-19688	NN	denotes	BNT77
T5287	19689-19692	IN	denotes	for
T5288	19693-19700	NN	denotes	capture
T5289	19701-19702	-LRB-	denotes	(
T5290	19710-19714	NN	denotes	Aβ11
T5291	19702-19709	NN	denotes	epitope
T5292	19714-19715	HYPH	denotes	–
T5293	19715-19717	CD	denotes	28
T5294	19717-19718	-RRB-	denotes	)
T5295	19719-19722	CC	denotes	and
T5296	19723-19727	NN	denotes	BA27
T5297	19728-19731	CC	denotes	and
T5298	19732-19736	NN	denotes	BC05
T5299	19737-19740	IN	denotes	for
T5300	19741-19750	NN	denotes	detection
T5301	19750-19751	.	denotes	.
T5302	19751-19848	sentence	denotes	All values were calculated as picomoles per gram based on the initial weight of cortical tissue.
T5303	19752-19755	DT	denotes	All
T5304	19756-19762	NNS	denotes	values
T5305	19768-19778	VBN	denotes	calculated
T5306	19763-19767	VBD	denotes	were
T5307	19779-19781	IN	denotes	as
T5308	19782-19791	NNS	denotes	picomoles
T5309	19792-19795	IN	denotes	per
T5310	19796-19800	NN	denotes	gram
T5311	19801-19806	VBN	denotes	based
T5312	19807-19809	IN	denotes	on
T5313	19810-19813	DT	denotes	the
T5314	19822-19828	NN	denotes	weight
T5315	19814-19821	JJ	denotes	initial
T5316	19829-19831	IN	denotes	of
T5317	19832-19840	JJ	denotes	cortical
T5318	19841-19847	NN	denotes	tissue
T5319	19847-19848	.	denotes	.
T5339	19850-19858	NN	denotes	Activity
T5340	19859-19869	NN	denotes	Monitoring
T5341	19869-19978	sentence	denotes	Daily basal activity was studied in 28 CaMKIIα-tTA × tet-APPswe/ind line 107 mice between 4 and 5 mo of age.
T5342	19870-19875	JJ	denotes	Daily
T5343	19882-19890	NN	denotes	activity
T5344	19876-19881	JJ	denotes	basal
T5345	19895-19902	VBN	denotes	studied
T5346	19891-19894	VBD	denotes	was
T5347	19903-19905	IN	denotes	in
T5348	19906-19908	CD	denotes	28
T5349	19947-19951	NNS	denotes	mice
T5350	19909-19916	NN	denotes	CaMKIIα
T5351	19934-19937	NN	denotes	ind
T5352	19916-19917	HYPH	denotes	-
T5353	19917-19920	NN	denotes	tTA
T5354	19921-19922	SYM	denotes	×
T5355	19923-19926	NN	denotes	tet
T5356	19926-19927	HYPH	denotes	-
T5357	19927-19933	NN	denotes	APPswe
T5358	19933-19934	HYPH	denotes	/
T5359	19938-19942	NN	denotes	line
T5360	19943-19946	CD	denotes	107
T5361	19952-19959	IN	denotes	between
T5362	19960-19961	CD	denotes	4
T5363	19968-19970	NN	denotes	mo
T5364	19962-19965	CC	denotes	and
T5365	19966-19967	CD	denotes	5
T5366	19971-19973	IN	denotes	of
T5367	19974-19977	NN	denotes	age
T5368	19977-19978	.	denotes	.
T5369	19978-20134	sentence	denotes	Animals were separated into individual cages immediately before the start of each experiment (n = 3–6 per genotype untreated, 2–5 per genotype dox-reared).
T5370	19979-19986	NNS	denotes	Animals
T5371	19992-20001	VBN	denotes	separated
T5372	19987-19991	VBD	denotes	were
T5373	20002-20006	IN	denotes	into
T5374	20007-20017	JJ	denotes	individual
T5375	20018-20023	NNS	denotes	cages
T5376	20024-20035	RB	denotes	immediately
T5377	20036-20042	IN	denotes	before
T5378	20043-20046	DT	denotes	the
T5379	20047-20052	NN	denotes	start
T5380	20053-20055	IN	denotes	of
T5381	20056-20060	DT	denotes	each
T5382	20061-20071	NN	denotes	experiment
T5383	20072-20073	-LRB-	denotes	(
T5384	20079-20080	CD	denotes	6
T5385	20073-20074	NN	denotes	n
T5386	20075-20076	SYM	denotes	=
T5387	20077-20078	CD	denotes	3
T5388	20078-20079	SYM	denotes	–
T5389	20081-20084	IN	denotes	per
T5390	20085-20093	NN	denotes	genotype
T5391	20094-20103	JJ	denotes	untreated
T5392	20103-20105	,	denotes	,
T5393	20105-20106	CD	denotes	2
T5394	20107-20108	CD	denotes	5
T5395	20106-20107	SYM	denotes	–
T5396	20109-20112	IN	denotes	per
T5397	20113-20121	NN	denotes	genotype
T5398	20122-20125	NN	denotes	dox
T5399	20126-20132	VBN	denotes	reared
T5400	20125-20126	HYPH	denotes	-
T5401	20132-20133	-RRB-	denotes	)
T5402	20133-20134	.	denotes	.
T5403	20134-20363	sentence	denotes	The cages were placed inside activity-monitoring frames designed to count every time the animal passed through one of three photobeams spanning the width of the cage (San Diego Instruments, San Diego, California, United States).
T5404	20135-20138	DT	denotes	The
T5405	20139-20144	NNS	denotes	cages
T5406	20150-20156	VBN	denotes	placed
T5407	20145-20149	VBD	denotes	were
T5408	20157-20163	IN	denotes	inside
T5409	20164-20172	NN	denotes	activity
T5410	20173-20183	VBG	denotes	monitoring
T5411	20172-20173	HYPH	denotes	-
T5412	20184-20190	NNS	denotes	frames
T5413	20191-20199	VBN	denotes	designed
T5414	20200-20202	TO	denotes	to
T5415	20203-20208	VB	denotes	count
T5416	20209-20214	DT	denotes	every
T5417	20215-20219	NN	denotes	time
T5418	20220-20223	DT	denotes	the
T5419	20224-20230	NN	denotes	animal
T5420	20231-20237	VBN	denotes	passed
T5421	20238-20245	IN	denotes	through
T5422	20246-20249	CD	denotes	one
T5423	20250-20252	IN	denotes	of
T5424	20253-20258	CD	denotes	three
T5425	20259-20269	NNS	denotes	photobeams
T5426	20270-20278	VBG	denotes	spanning
T5427	20279-20282	DT	denotes	the
T5428	20283-20288	NN	denotes	width
T5429	20289-20291	IN	denotes	of
T5430	20292-20295	DT	denotes	the
T5431	20296-20300	NN	denotes	cage
T5432	20301-20302	-LRB-	denotes	(
T5433	20312-20323	NNP	denotes	Instruments
T5434	20302-20305	NNP	denotes	San
T5435	20306-20311	NNP	denotes	Diego
T5436	20323-20325	,	denotes	,
T5437	20325-20328	NNP	denotes	San
T5438	20329-20334	NNP	denotes	Diego
T5439	20334-20336	,	denotes	,
T5440	20336-20346	NNP	denotes	California
T5441	20346-20348	,	denotes	,
T5442	20348-20354	NNP	denotes	United
T5443	20355-20361	NNP	denotes	States
T5444	20361-20362	-RRB-	denotes	)
T5445	20362-20363	.	denotes	.
T5446	20363-20491	sentence	denotes	Experiments were started midway through the light phase of the day, and data were collected in 1-h bins for the following 48 h.
T5447	20364-20375	NNS	denotes	Experiments
T5448	20381-20388	VBN	denotes	started
T5449	20376-20380	VBD	denotes	were
T5450	20389-20395	RB	denotes	midway
T5451	20396-20403	IN	denotes	through
T5452	20404-20407	DT	denotes	the
T5453	20414-20419	NN	denotes	phase
T5454	20408-20413	NN	denotes	light
T5455	20420-20422	IN	denotes	of
T5456	20423-20426	DT	denotes	the
T5457	20427-20430	NN	denotes	day
T5458	20430-20432	,	denotes	,
T5459	20432-20435	CC	denotes	and
T5460	20436-20440	NNS	denotes	data
T5461	20446-20455	VBN	denotes	collected
T5462	20441-20445	VBD	denotes	were
T5463	20456-20458	IN	denotes	in
T5464	20459-20460	CD	denotes	1
T5465	20461-20462	NN	denotes	h
T5466	20460-20461	HYPH	denotes	-
T5467	20463-20467	NNS	denotes	bins
T5468	20468-20471	IN	denotes	for
T5469	20472-20475	DT	denotes	the
T5470	20489-20490	NN	denotes	h
T5471	20476-20485	JJ	denotes	following
T5472	20486-20488	CD	denotes	48
T5473	20490-20491	.	denotes	.
T5474	20491-20638	sentence	denotes	Testing rooms were maintained on the same 13:11 h day:night cycle as the main animal housing areas and were closed to entry during the experiment.
T5475	20492-20499	NN	denotes	Testing
T5476	20500-20505	NNS	denotes	rooms
T5477	20511-20521	VBN	denotes	maintained
T5478	20506-20510	VBD	denotes	were
T5479	20522-20524	IN	denotes	on
T5480	20525-20528	DT	denotes	the
T5481	20552-20557	NN	denotes	cycle
T5482	20529-20533	JJ	denotes	same
T5483	20534-20536	CD	denotes	13
T5484	20540-20541	NN	denotes	h
T5485	20536-20537	SYM	denotes	:
T5486	20537-20539	CD	denotes	11
T5487	20542-20545	NN	denotes	day
T5488	20545-20546	SYM	denotes	:
T5489	20546-20551	NN	denotes	night
T5490	20558-20560	IN	denotes	as
T5491	20561-20564	DT	denotes	the
T5492	20585-20590	NNS	denotes	areas
T5493	20565-20569	JJ	denotes	main
T5494	20570-20576	NN	denotes	animal
T5495	20577-20584	NN	denotes	housing
T5496	20591-20594	CC	denotes	and
T5497	20595-20599	VBD	denotes	were
T5498	20600-20606	VBN	denotes	closed
T5499	20607-20609	IN	denotes	to
T5500	20610-20615	NN	denotes	entry
T5501	20616-20622	IN	denotes	during
T5502	20623-20626	DT	denotes	the
T5503	20627-20637	NN	denotes	experiment
T5504	20637-20638	.	denotes	.
T5510	20640-20651	JJ	denotes	Statistical
T5511	20652-20660	NNS	denotes	Analyses
T5512	20660-20932	sentence	denotes	Statistical analyses of protein expression, ELISA data, and filter trap assays were performed by ANOVA with Tukey's honest significant difference post-hoc test applied to significant main effects or interactions (Statistica 6.0, StatSoft, Tulsa, Oklahoma, United States).
T5513	20661-20672	JJ	denotes	Statistical
T5514	20673-20681	NNS	denotes	analyses
T5515	20745-20754	VBN	denotes	performed
T5516	20682-20684	IN	denotes	of
T5517	20685-20692	NN	denotes	protein
T5518	20693-20703	NN	denotes	expression
T5519	20703-20705	,	denotes	,
T5520	20705-20710	NN	denotes	ELISA
T5521	20711-20715	NNS	denotes	data
T5522	20715-20717	,	denotes	,
T5523	20717-20720	CC	denotes	and
T5524	20721-20727	NN	denotes	filter
T5525	20728-20732	NN	denotes	trap
T5526	20733-20739	NNS	denotes	assays
T5527	20740-20744	VBD	denotes	were
T5528	20755-20757	IN	denotes	by
T5529	20758-20763	NN	denotes	ANOVA
T5530	20764-20768	IN	denotes	with
T5531	20769-20774	NNP	denotes	Tukey
T5532	20816-20820	NN	denotes	test
T5533	20774-20776	POS	denotes	's
T5534	20777-20783	JJ	denotes	honest
T5535	20784-20795	JJ	denotes	significant
T5536	20796-20806	NN	denotes	difference
T5537	20807-20811	FW	denotes	post
T5538	20812-20815	FW	denotes	hoc
T5539	20811-20812	HYPH	denotes	-
T5540	20821-20828	VBN	denotes	applied
T5541	20829-20831	IN	denotes	to
T5542	20832-20843	JJ	denotes	significant
T5543	20849-20856	NNS	denotes	effects
T5544	20844-20848	JJ	denotes	main
T5545	20857-20859	CC	denotes	or
T5546	20860-20872	NNS	denotes	interactions
T5547	20873-20874	-LRB-	denotes	(
T5548	20874-20884	NNP	denotes	Statistica
T5549	20885-20888	CD	denotes	6.0
T5550	20888-20890	,	denotes	,
T5551	20890-20898	NNP	denotes	StatSoft
T5552	20898-20900	,	denotes	,
T5553	20900-20905	NNP	denotes	Tulsa
T5554	20905-20907	,	denotes	,
T5555	20907-20915	NNP	denotes	Oklahoma
T5556	20915-20917	,	denotes	,
T5557	20917-20923	NNP	denotes	United
T5558	20924-20930	NNP	denotes	States
T5559	20930-20931	-RRB-	denotes	)
T5560	20931-20932	.	denotes	.
T5561	20932-21072	sentence	denotes	In cases of positively skewed data distribution, log10(x + 0.5) transformation was applied to the raw data before submitting them to ANOVA.
T5562	20933-20935	IN	denotes	In
T5563	21016-21023	VBN	denotes	applied
T5564	20936-20941	NNS	denotes	cases
T5565	20942-20944	IN	denotes	of
T5566	20945-20955	RB	denotes	positively
T5567	20956-20962	VBN	denotes	skewed
T5568	20968-20980	NN	denotes	distribution
T5569	20963-20967	NNS	denotes	data
T5570	20980-20982	,	denotes	,
T5571	20982-20987	NN	denotes	log10
T5572	20988-20989	NN	denotes	x
T5573	20987-20988	-LRB-	denotes	(
T5574	20997-21011	NN	denotes	transformation
T5575	20990-20991	SYM	denotes	+
T5576	20992-20995	CD	denotes	0.5
T5577	20995-20996	-RRB-	denotes	)
T5578	21012-21015	VBD	denotes	was
T5579	21024-21026	IN	denotes	to
T5580	21027-21030	DT	denotes	the
T5581	21035-21039	NNS	denotes	data
T5582	21031-21034	JJ	denotes	raw
T5583	21040-21046	IN	denotes	before
T5584	21047-21057	VBG	denotes	submitting
T5585	21058-21062	PRP	denotes	them
T5586	21063-21065	IN	denotes	to
T5587	21066-21071	NN	denotes	ANOVA
T5588	21071-21072	.	denotes	.
T6511	21082-21084	PRP	denotes	We
T6512	21085-21089	VBD	denotes	used
T6513	21090-21093	DT	denotes	the
T6514	21112-21118	NN	denotes	system
T6515	21094-21097	NN	denotes	tet
T6516	21098-21101	NN	denotes	off
T6517	21097-21098	HYPH	denotes	-
T6518	21102-21111	NN	denotes	transgene
T6519	21119-21121	TO	denotes	to
T6520	21122-21129	VB	denotes	express
T6521	21130-21131	DT	denotes	a
T6522	21146-21153	NN	denotes	version
T6523	21132-21138	JJ	denotes	double
T6524	21139-21145	NN	denotes	mutant
T6525	21154-21156	IN	denotes	of
T6526	21157-21165	JJ	denotes	chimeric
T6527	21169-21177	NN	denotes	huAPP695
T6528	21166-21168	NN	denotes	mo
T6529	21168-21169	HYPH	denotes	/
T6530	21178-21179	-LRB-	denotes	(
T6531	21179-21182	NN	denotes	swe
T6532	21187-21192	NN	denotes	KM570
T6533	21182-21183	HYPH	denotes	/
T6534	21183-21186	NN	denotes	ind
T6535	21192-21194	,	denotes	,
T6536	21194-21199	NN	denotes	571NL
T6537	21199-21201	,	denotes	,
T6538	21201-21204	CC	denotes	and
T6539	21205-21210	NN	denotes	V617F
T6540	21210-21211	-RRB-	denotes	)
T6541	21212-21216	IN	denotes	from
T6542	21217-21218	DT	denotes	a
T6543	21243-21251	NN	denotes	promoter
T6544	21219-21231	NN	denotes	tetracycline
T6545	21232-21242	JJ	denotes	responsive
T6546	21231-21232	HYPH	denotes	-
T6547	21252-21253	-LRB-	denotes	[
T6548	21256-21258	CD	denotes	13
T6549	21253-21255	CD	denotes	12
T6550	21255-21256	,	denotes	,
T6551	21258-21259	-RRB-	denotes	]
T6552	21259-21260	.	denotes	.
T6553	21260-21401	sentence	denotes	Transgenic APP expression was activated by crossing the APPswe/ind mice to animals producing tTA under control of the CaMKIIα promoter [16].
T6554	21261-21271	JJ	denotes	Transgenic
T6555	21272-21275	NN	denotes	APP
T6556	21276-21286	NN	denotes	expression
T6557	21291-21300	VBN	denotes	activated
T6558	21287-21290	VBD	denotes	was
T6559	21301-21303	IN	denotes	by
T6560	21304-21312	VBG	denotes	crossing
T6561	21313-21316	DT	denotes	the
T6562	21328-21332	NNS	denotes	mice
T6563	21317-21323	NN	denotes	APPswe
T6564	21324-21327	NN	denotes	ind
T6565	21323-21324	HYPH	denotes	/
T6566	21333-21335	IN	denotes	to
T6567	21336-21343	NNS	denotes	animals
T6568	21344-21353	VBG	denotes	producing
T6569	21354-21357	NN	denotes	tTA
T6570	21358-21363	IN	denotes	under
T6571	21364-21371	NN	denotes	control
T6572	21372-21374	IN	denotes	of
T6573	21375-21378	DT	denotes	the
T6574	21387-21395	NN	denotes	promoter
T6575	21379-21386	NN	denotes	CaMKIIα
T6576	21396-21397	-LRB-	denotes	[
T6577	21397-21399	CD	denotes	16
T6578	21399-21400	-RRB-	denotes	]
T6579	21400-21401	.	denotes	.
T6580	21401-21595	sentence	denotes	After initial screening of founders, we identified four lines of tet-APPswe/ind mice that produced very high levels of transgene product in offspring coexpressing tTA (Figures 1 and Figure S1).
T6581	21402-21407	IN	denotes	After
T6582	21442-21452	VBD	denotes	identified
T6583	21408-21415	JJ	denotes	initial
T6584	21416-21425	NN	denotes	screening
T6585	21426-21428	IN	denotes	of
T6586	21429-21437	NNS	denotes	founders
T6587	21437-21439	,	denotes	,
T6588	21439-21441	PRP	denotes	we
T6589	21453-21457	CD	denotes	four
T6590	21458-21463	NNS	denotes	lines
T6591	21464-21466	IN	denotes	of
T6592	21467-21470	NN	denotes	tet
T6593	21478-21481	NN	denotes	ind
T6594	21470-21471	HYPH	denotes	-
T6595	21471-21477	NN	denotes	APPswe
T6596	21477-21478	HYPH	denotes	/
T6597	21482-21486	NNS	denotes	mice
T6598	21487-21491	WDT	denotes	that
T6599	21492-21500	VBD	denotes	produced
T6600	21501-21505	RB	denotes	very
T6601	21506-21510	JJ	denotes	high
T6602	21511-21517	NNS	denotes	levels
T6603	21518-21520	IN	denotes	of
T6604	21521-21530	NN	denotes	transgene
T6605	21531-21538	NN	denotes	product
T6606	21539-21541	IN	denotes	in
T6607	21542-21551	NN	denotes	offspring
T6608	21552-21564	VBG	denotes	coexpressing
T6609	21565-21568	NN	denotes	tTA
T6610	21569-21570	-LRB-	denotes	(
T6611	21570-21577	NNS	denotes	Figures
T6612	21578-21579	CD	denotes	1
T6613	21580-21583	CC	denotes	and
T6614	21584-21590	NN	denotes	Figure
T6615	21591-21593	NN	denotes	S1
T6616	21593-21594	-RRB-	denotes	)
T6617	21594-21595	.	denotes	.
T6618	21595-21846	sentence	denotes	Compared to a standard APP transgenic line used for previous amyloid studies by our laboratory (line C3–3; [15,26,27]), we estimated that the four controllable lines produce transgenic APP protein at 10- to 30-fold over endogenous levels (Figure S1).
T6619	21596-21604	VBN	denotes	Compared
T6620	21719-21728	VBD	denotes	estimated
T6621	21605-21607	IN	denotes	to
T6622	21608-21609	DT	denotes	a
T6623	21634-21638	NN	denotes	line
T6624	21610-21618	JJ	denotes	standard
T6625	21619-21622	NN	denotes	APP
T6626	21623-21633	JJ	denotes	transgenic
T6627	21639-21643	VBN	denotes	used
T6628	21644-21647	IN	denotes	for
T6629	21648-21656	JJ	denotes	previous
T6630	21665-21672	NNS	denotes	studies
T6631	21657-21664	NN	denotes	amyloid
T6632	21673-21675	IN	denotes	by
T6633	21676-21679	PRP$	denotes	our
T6634	21680-21690	NN	denotes	laboratory
T6635	21691-21692	-LRB-	denotes	(
T6636	21710-21712	CD	denotes	27
T6637	21692-21696	NN	denotes	line
T6638	21697-21699	NN	denotes	C3
T6639	21699-21700	HYPH	denotes	–
T6640	21700-21701	CD	denotes	3
T6641	21701-21702	:	denotes	;
T6642	21703-21704	-LRB-	denotes	[
T6643	21704-21706	CD	denotes	15
T6644	21706-21707	,	denotes	,
T6645	21707-21709	CD	denotes	26
T6646	21709-21710	,	denotes	,
T6647	21712-21713	-RRB-	denotes	]
T6648	21713-21714	-RRB-	denotes	)
T6649	21714-21716	,	denotes	,
T6650	21716-21718	PRP	denotes	we
T6651	21729-21733	IN	denotes	that
T6652	21762-21769	VBP	denotes	produce
T6653	21734-21737	DT	denotes	the
T6654	21756-21761	NNS	denotes	lines
T6655	21738-21742	CD	denotes	four
T6656	21743-21755	JJ	denotes	controllable
T6657	21770-21780	JJ	denotes	transgenic
T6658	21785-21792	NN	denotes	protein
T6659	21781-21784	NN	denotes	APP
T6660	21793-21795	IN	denotes	at
T6661	21796-21798	CD	denotes	10
T6662	21798-21799	HYPH	denotes	-
T6663	21800-21802	IN	denotes	to
T6664	21803-21810	RB	denotes	30-fold
T6665	21811-21815	IN	denotes	over
T6666	21816-21826	JJ	denotes	endogenous
T6667	21827-21833	NNS	denotes	levels
T6668	21834-21835	-LRB-	denotes	(
T6669	21842-21844	NN	denotes	S1
T6670	21835-21841	NN	denotes	Figure
T6671	21844-21845	-RRB-	denotes	)
T6672	21845-21846	.	denotes	.
T6673	21846-22107	sentence	denotes	This estimate was confirmed by direct comparison of APP levels in nontransgenic and tet-off APP mice using an antibody that recognizes both endogenous APP (and amyloid precursor-like protein 2) and the transgenic protein (monoclonal antibody 22C11; Figure 1D).
T6674	21847-21851	DT	denotes	This
T6675	21852-21860	NN	denotes	estimate
T6676	21865-21874	VBN	denotes	confirmed
T6677	21861-21864	VBD	denotes	was
T6678	21875-21877	IN	denotes	by
T6679	21878-21884	JJ	denotes	direct
T6680	21885-21895	NN	denotes	comparison
T6681	21896-21898	IN	denotes	of
T6682	21899-21902	NN	denotes	APP
T6683	21903-21909	NNS	denotes	levels
T6684	21910-21912	IN	denotes	in
T6685	21913-21926	JJ	denotes	nontransgenic
T6686	21943-21947	NNS	denotes	mice
T6687	21927-21930	CC	denotes	and
T6688	21931-21934	NN	denotes	tet
T6689	21935-21938	NN	denotes	off
T6690	21934-21935	HYPH	denotes	-
T6691	21939-21942	NN	denotes	APP
T6692	21948-21953	VBG	denotes	using
T6693	21954-21956	DT	denotes	an
T6694	21957-21965	NN	denotes	antibody
T6695	21966-21970	WDT	denotes	that
T6696	21971-21981	VBZ	denotes	recognizes
T6697	21982-21986	CC	denotes	both
T6698	21998-22001	NN	denotes	APP
T6699	21987-21997	JJ	denotes	endogenous
T6700	22002-22003	-LRB-	denotes	(
T6701	22003-22006	CC	denotes	and
T6702	22007-22014	NN	denotes	amyloid
T6703	22030-22037	NN	denotes	protein
T6704	22015-22024	NN	denotes	precursor
T6705	22025-22029	JJ	denotes	like
T6706	22024-22025	HYPH	denotes	-
T6707	22038-22039	CD	denotes	2
T6708	22039-22040	-RRB-	denotes	)
T6709	22041-22044	CC	denotes	and
T6710	22045-22048	DT	denotes	the
T6711	22060-22067	NN	denotes	protein
T6712	22049-22059	JJ	denotes	transgenic
T6713	22068-22069	-LRB-	denotes	(
T6714	22103-22105	NN	denotes	1D
T6715	22069-22079	JJ	denotes	monoclonal
T6716	22089-22094	NN	denotes	22C11
T6717	22080-22088	NN	denotes	antibody
T6718	22094-22095	:	denotes	;
T6719	22096-22102	NN	denotes	Figure
T6720	22105-22106	-RRB-	denotes	)
T6721	22106-22107	.	denotes	.
T6722	22107-23908	sentence	denotes	Figure 1 Control of Transgenic APP Expression by Dox (A) Western blotting for transgenic APP using the human-specific 6E10 antibody shows expression of full-length protein in forebrain tissue from young predeposit double transgenic animals (line 107) and its suppression following dox treatment. Untreated animals show high levels of transgene expression; protein levels drop dramatically in animals acutely treated with dox for 2 wk. A faint, but detectable band of full-length protein remains in acutely treated animals that can be eliminated in mice born and raised on dox. (B) Immunoblotting with the N-terminal antibody 22C11 to detect both transgenic and endogenous protein shows that dox treatment reduces APP/APLP to levels found in nontransgenic mice. (C) Measurement of signal intensity from the Western blot in (A) shows transgenic APP levels are decreased more than 95% by dox in both acutely and chronically treated animals (97.2% for 4 wk + 2 wk dox, 98.0% for reared on dox versus 4 wk untreated; ANOVA effect of treatment group F 4,15 = 85.55, p < 0.001). APP levels in 4 wk + 2 wk dox, reared on dox, and nontransgenic (NTg) were not significantly different (p > 0.9, Tukey post-hoc test). (D) Measurement of signal intensity from the Western blot in (B) shows total APP/APLP levels in dox-treated tTA/APP mice are significantly lower than in 4-wk-old untreated mice (ANOVA effect of treatment group F 4,15 = 84.41, p < 0.001) and indistinguishable from those of nontransgenic animals (p > 0.9, Tukey post-hoc test). *, p < 0.001 versus untreated 4-wk-old mice, Tukey post-hoc test applied to significant effect of group ANOVA. Importantly, all four new lines of tet-off APP mice showed nearly complete suppression of the transgene following dox treatment (Figures 1 and Figure S1).
T6723	23754-23765	RB	denotes	Importantly
T6724	23806-23812	VBD	denotes	showed
T6725	23765-23767	,	denotes	,
T6726	23767-23770	DT	denotes	all
T6727	23780-23785	NNS	denotes	lines
T6728	23771-23775	CD	denotes	four
T6729	23776-23779	JJ	denotes	new
T6730	23786-23788	IN	denotes	of
T6731	23789-23792	NN	denotes	tet
T6732	23793-23796	NN	denotes	off
T6733	23792-23793	HYPH	denotes	-
T6734	23801-23805	NNS	denotes	mice
T6735	23797-23800	NN	denotes	APP
T6736	23813-23819	RB	denotes	nearly
T6737	23820-23828	JJ	denotes	complete
T6738	23829-23840	NN	denotes	suppression
T6739	23841-23843	IN	denotes	of
T6740	23844-23847	DT	denotes	the
T6741	23848-23857	NN	denotes	transgene
T6742	23858-23867	VBG	denotes	following
T6743	23868-23871	NN	denotes	dox
T6744	23872-23881	NN	denotes	treatment
T6745	23882-23883	-LRB-	denotes	(
T6746	23883-23890	NNS	denotes	Figures
T6747	23891-23892	CD	denotes	1
T6748	23893-23896	CC	denotes	and
T6749	23897-23903	NN	denotes	Figure
T6750	23904-23906	NN	denotes	S1
T6751	23906-23907	-RRB-	denotes	)
T6752	23907-23908	.	denotes	.
T6753	23908-24091	sentence	denotes	We focused on one of the four lines, line 107, to examine in more detail the time dependence and extent of transgene suppression following either acute or chronic treatment with dox.
T6754	23909-23911	PRP	denotes	We
T6755	23912-23919	VBD	denotes	focused
T6756	23920-23922	IN	denotes	on
T6757	23923-23926	CD	denotes	one
T6758	23927-23929	IN	denotes	of
T6759	23930-23933	DT	denotes	the
T6760	23939-23944	NNS	denotes	lines
T6761	23934-23938	CD	denotes	four
T6762	23944-23946	,	denotes	,
T6763	23946-23950	NN	denotes	line
T6764	23951-23954	CD	denotes	107
T6765	23954-23956	,	denotes	,
T6766	23956-23958	TO	denotes	to
T6767	23959-23966	VB	denotes	examine
T6768	23967-23969	IN	denotes	in
T6769	23970-23974	JJR	denotes	more
T6770	23975-23981	NN	denotes	detail
T6771	23982-23985	DT	denotes	the
T6772	23991-24001	NN	denotes	dependence
T6773	23986-23990	NN	denotes	time
T6774	24002-24005	CC	denotes	and
T6775	24006-24012	NN	denotes	extent
T6776	24013-24015	IN	denotes	of
T6777	24016-24025	NN	denotes	transgene
T6778	24026-24037	NN	denotes	suppression
T6779	24038-24047	VBG	denotes	following
T6780	24048-24054	CC	denotes	either
T6781	24072-24081	NN	denotes	treatment
T6782	24055-24060	JJ	denotes	acute
T6783	24061-24063	CC	denotes	or
T6784	24064-24071	JJ	denotes	chronic
T6785	24082-24086	IN	denotes	with
T6786	24087-24090	NN	denotes	dox
T6787	24090-24091	.	denotes	.
T6788	24091-24371	sentence	denotes	Two dox-treated groups were compared to two untreated groups: one group of mice was born and raised on dox, a second group was treated with dox for 2 wk starting at 1 mo of age (4 wk + 2 wk dox); two untreated groups kept on normal chow were harvested at either 4 or 6 wk of age.
T6789	24092-24095	CD	denotes	Two
T6790	24108-24114	NNS	denotes	groups
T6791	24096-24099	NN	denotes	dox
T6792	24100-24107	VBN	denotes	treated
T6793	24099-24100	HYPH	denotes	-
T6794	24120-24128	VBN	denotes	compared
T6795	24115-24119	VBD	denotes	were
T6796	24334-24343	VBN	denotes	harvested
T6797	24129-24131	IN	denotes	to
T6798	24132-24135	CD	denotes	two
T6799	24146-24152	NNS	denotes	groups
T6800	24136-24145	JJ	denotes	untreated
T6801	24152-24154	:	denotes	:
T6802	24154-24157	CD	denotes	one
T6803	24158-24163	NN	denotes	group
T6804	24176-24180	VBN	denotes	born
T6805	24164-24166	IN	denotes	of
T6806	24167-24171	NNS	denotes	mice
T6807	24172-24175	VBD	denotes	was
T6808	24181-24184	CC	denotes	and
T6809	24185-24191	VBN	denotes	raised
T6810	24192-24194	IN	denotes	on
T6811	24195-24198	NN	denotes	dox
T6812	24198-24200	,	denotes	,
T6813	24200-24201	DT	denotes	a
T6814	24209-24214	NN	denotes	group
T6815	24202-24208	JJ	denotes	second
T6816	24219-24226	VBN	denotes	treated
T6817	24215-24218	VBD	denotes	was
T6818	24227-24231	IN	denotes	with
T6819	24232-24235	NN	denotes	dox
T6820	24236-24239	IN	denotes	for
T6821	24240-24241	CD	denotes	2
T6822	24242-24244	NN	denotes	wk
T6823	24245-24253	VBG	denotes	starting
T6824	24254-24256	IN	denotes	at
T6825	24257-24258	CD	denotes	1
T6826	24259-24261	NN	denotes	mo
T6827	24262-24264	IN	denotes	of
T6828	24265-24268	NN	denotes	age
T6829	24269-24270	-LRB-	denotes	(
T6830	24272-24274	NN	denotes	wk
T6831	24270-24271	CD	denotes	4
T6832	24275-24276	SYM	denotes	+
T6833	24277-24278	CD	denotes	2
T6834	24279-24281	NN	denotes	wk
T6835	24282-24285	NN	denotes	dox
T6836	24285-24286	-RRB-	denotes	)
T6837	24286-24287	:	denotes	;
T6838	24288-24291	CD	denotes	two
T6839	24302-24308	NNS	denotes	groups
T6840	24292-24301	JJ	denotes	untreated
T6841	24309-24313	VBN	denotes	kept
T6842	24314-24316	IN	denotes	on
T6843	24317-24323	JJ	denotes	normal
T6844	24324-24328	NN	denotes	chow
T6845	24329-24333	VBD	denotes	were
T6846	24344-24346	IN	denotes	at
T6847	24347-24353	CC	denotes	either
T6848	24354-24355	CD	denotes	4
T6849	24361-24363	NN	denotes	wk
T6850	24356-24358	CC	denotes	or
T6851	24359-24360	CD	denotes	6
T6852	24364-24366	IN	denotes	of
T6853	24367-24370	NN	denotes	age
T6854	24370-24371	.	denotes	.
T6855	24371-24442	sentence	denotes	Animals born and raised on dox harbored no transgenic APP (Figure 1A).
T6856	24372-24379	NNS	denotes	Animals
T6857	24403-24411	VBD	denotes	harbored
T6858	24380-24384	VBN	denotes	born
T6859	24385-24388	CC	denotes	and
T6860	24389-24395	VBN	denotes	raised
T6861	24396-24398	IN	denotes	on
T6862	24399-24402	NN	denotes	dox
T6863	24412-24414	DT	denotes	no
T6864	24426-24429	NN	denotes	APP
T6865	24415-24425	JJ	denotes	transgenic
T6866	24430-24431	-LRB-	denotes	(
T6867	24438-24440	NN	denotes	1A
T6868	24431-24437	NN	denotes	Figure
T6869	24440-24441	-RRB-	denotes	)
T6870	24441-24442	.	denotes	.
T6871	24442-24571	sentence	denotes	Following as little as 2 wk of dox treatment, transgenic APP expression was reduced by more than 95% compared to pre-dox levels.
T6872	24443-24452	VBG	denotes	Following
T6873	24519-24526	VBN	denotes	reduced
T6874	24453-24455	RB	denotes	as
T6875	24466-24467	CD	denotes	2
T6876	24456-24462	JJ	denotes	little
T6877	24463-24465	IN	denotes	as
T6878	24468-24470	NN	denotes	wk
T6879	24471-24473	IN	denotes	of
T6880	24474-24477	NN	denotes	dox
T6881	24478-24487	NN	denotes	treatment
T6882	24487-24489	,	denotes	,
T6883	24489-24499	JJ	denotes	transgenic
T6884	24504-24514	NN	denotes	expression
T6885	24500-24503	NN	denotes	APP
T6886	24515-24518	VBD	denotes	was
T6887	24527-24529	IN	denotes	by
T6888	24530-24534	JJR	denotes	more
T6889	24540-24542	CD	denotes	95
T6890	24535-24539	IN	denotes	than
T6891	24542-24543	NN	denotes	%
T6892	24544-24552	VBN	denotes	compared
T6893	24553-24555	IN	denotes	to
T6894	24556-24563	JJ	denotes	pre-dox
T6895	24564-24570	NNS	denotes	levels
T6896	24570-24571	.	denotes	.
T6897	24571-24810	sentence	denotes	The residual expression remaining in acutely treated mice represents less than 4% of the transgenic protein produced in the absence of dox (Figure 1C), and likely results from slight leakage at the level of transcription (data not shown).
T6898	24572-24575	DT	denotes	The
T6899	24585-24595	NN	denotes	expression
T6900	24576-24584	JJ	denotes	residual
T6901	24630-24640	VBZ	denotes	represents
T6902	24596-24605	VBG	denotes	remaining
T6903	24606-24608	IN	denotes	in
T6904	24609-24616	RB	denotes	acutely
T6905	24617-24624	VBN	denotes	treated
T6906	24625-24629	NNS	denotes	mice
T6907	24641-24645	JJR	denotes	less
T6908	24651-24652	CD	denotes	4
T6909	24646-24650	IN	denotes	than
T6910	24652-24653	NN	denotes	%
T6911	24654-24656	IN	denotes	of
T6912	24657-24660	DT	denotes	the
T6913	24672-24679	NN	denotes	protein
T6914	24661-24671	JJ	denotes	transgenic
T6915	24680-24688	VBN	denotes	produced
T6916	24689-24691	IN	denotes	in
T6917	24692-24695	DT	denotes	the
T6918	24696-24703	NN	denotes	absence
T6919	24704-24706	IN	denotes	of
T6920	24707-24710	NN	denotes	dox
T6921	24711-24712	-LRB-	denotes	(
T6922	24719-24721	NN	denotes	1C
T6923	24712-24718	NN	denotes	Figure
T6924	24721-24722	-RRB-	denotes	)
T6925	24722-24724	,	denotes	,
T6926	24724-24727	CC	denotes	and
T6927	24728-24734	RB	denotes	likely
T6928	24735-24742	VBZ	denotes	results
T6929	24743-24747	IN	denotes	from
T6930	24748-24754	JJ	denotes	slight
T6931	24755-24762	NN	denotes	leakage
T6932	24763-24765	IN	denotes	at
T6933	24766-24769	DT	denotes	the
T6934	24770-24775	NN	denotes	level
T6935	24776-24778	IN	denotes	of
T6936	24779-24792	NN	denotes	transcription
T6937	24793-24794	-LRB-	denotes	(
T6938	24803-24808	VBN	denotes	shown
T6939	24794-24798	NNS	denotes	data
T6940	24799-24802	RB	denotes	not
T6941	24808-24809	-RRB-	denotes	)
T6942	24809-24810	.	denotes	.
T6943	24810-25129	sentence	denotes	Importantly, the total amount of APP (endogenous plus transgenic) and related APLPs in both acute and chronically treated animals was statistically indistinguishable from that in nontransgenic mice (Figure 1D; statistical analyses for experiments throughout the study are presented in the accompanying figure legends).
T6944	24811-24822	RB	denotes	Importantly
T6945	24941-24944	VBD	denotes	was
T6946	24822-24824	,	denotes	,
T6947	24824-24827	DT	denotes	the
T6948	24834-24840	NN	denotes	amount
T6949	24828-24833	JJ	denotes	total
T6950	24841-24843	IN	denotes	of
T6951	24844-24847	NN	denotes	APP
T6952	24848-24849	-LRB-	denotes	(
T6953	24849-24859	JJ	denotes	endogenous
T6954	24860-24864	CC	denotes	plus
T6955	24865-24875	JJ	denotes	transgenic
T6956	24875-24876	-RRB-	denotes	)
T6957	24877-24880	CC	denotes	and
T6958	24881-24888	JJ	denotes	related
T6959	24889-24894	NNS	denotes	APLPs
T6960	24895-24897	IN	denotes	in
T6961	24898-24902	CC	denotes	both
T6962	24903-24908	JJ	denotes	acute
T6963	24933-24940	NNS	denotes	animals
T6964	24909-24912	CC	denotes	and
T6965	24913-24924	RB	denotes	chronically
T6966	24925-24932	VBN	denotes	treated
T6967	24945-24958	RB	denotes	statistically
T6968	24959-24976	JJ	denotes	indistinguishable
T6969	24977-24981	IN	denotes	from
T6970	24982-24986	DT	denotes	that
T6971	24987-24989	IN	denotes	in
T6972	24990-25003	JJ	denotes	nontransgenic
T6973	25004-25008	NNS	denotes	mice
T6974	25009-25010	-LRB-	denotes	(
T6975	25017-25019	NN	denotes	1D
T6976	25010-25016	NN	denotes	Figure
T6977	25019-25020	:	denotes	;
T6978	25021-25032	JJ	denotes	statistical
T6979	25033-25041	NNS	denotes	analyses
T6980	25083-25092	VBN	denotes	presented
T6981	25042-25045	IN	denotes	for
T6982	25046-25057	NNS	denotes	experiments
T6983	25058-25068	IN	denotes	throughout
T6984	25069-25072	DT	denotes	the
T6985	25073-25078	NN	denotes	study
T6986	25079-25082	VBP	denotes	are
T6987	25093-25095	IN	denotes	in
T6988	25096-25099	DT	denotes	the
T6989	25120-25127	NNS	denotes	legends
T6990	25100-25112	VBG	denotes	accompanying
T6991	25113-25119	NN	denotes	figure
T6992	25127-25128	-RRB-	denotes	)
T6993	25128-25129	.	denotes	.
T6994	25129-25341	sentence	denotes	To ensure that Aβ production was suppressed in concert with the dox-mediated inhibition of its precursor APPswe/ind, we measured Aβ40 and Aβ42 levels by ELISA in forebrain homogenates from young tet-off animals.
T6995	25130-25132	TO	denotes	To
T6996	25133-25139	VB	denotes	ensure
T6997	25250-25258	VBD	denotes	measured
T6998	25140-25144	IN	denotes	that
T6999	25163-25173	VBN	denotes	suppressed
T7000	25145-25147	NN	denotes	Aβ
T7001	25148-25158	NN	denotes	production
T7002	25159-25162	VBD	denotes	was
T7003	25174-25176	IN	denotes	in
T7004	25177-25184	NN	denotes	concert
T7005	25185-25189	IN	denotes	with
T7006	25190-25193	DT	denotes	the
T7007	25207-25217	NN	denotes	inhibition
T7008	25194-25197	NN	denotes	dox
T7009	25198-25206	VBN	denotes	mediated
T7010	25197-25198	HYPH	denotes	-
T7011	25218-25220	IN	denotes	of
T7012	25221-25224	PRP$	denotes	its
T7013	25225-25234	NN	denotes	precursor
T7014	25235-25241	NN	denotes	APPswe
T7015	25242-25245	NN	denotes	ind
T7016	25241-25242	HYPH	denotes	/
T7017	25245-25247	,	denotes	,
T7018	25247-25249	PRP	denotes	we
T7019	25259-25263	NN	denotes	Aβ40
T7020	25273-25279	NNS	denotes	levels
T7021	25264-25267	CC	denotes	and
T7022	25268-25272	NN	denotes	Aβ42
T7023	25280-25282	IN	denotes	by
T7024	25283-25288	NN	denotes	ELISA
T7025	25289-25291	IN	denotes	in
T7026	25292-25301	NN	denotes	forebrain
T7027	25302-25313	NNS	denotes	homogenates
T7028	25314-25318	IN	denotes	from
T7029	25319-25324	JJ	denotes	young
T7030	25333-25340	NNS	denotes	animals
T7031	25325-25328	NN	denotes	tet
T7032	25329-25332	NN	denotes	off
T7033	25328-25329	HYPH	denotes	-
T7034	25340-25341	.	denotes	.
T7035	25341-25518	sentence	denotes	At 1 mo of age, the mice lacked visible amyloid aggregates that might act as an intractable reservoir of peptide remaining in the brain after the transgene had been suppressed.
T7036	25342-25344	IN	denotes	At
T7037	25367-25373	VBD	denotes	lacked
T7038	25345-25346	CD	denotes	1
T7039	25347-25349	NN	denotes	mo
T7040	25350-25352	IN	denotes	of
T7041	25353-25356	NN	denotes	age
T7042	25356-25358	,	denotes	,
T7043	25358-25361	DT	denotes	the
T7044	25362-25366	NNS	denotes	mice
T7045	25374-25381	JJ	denotes	visible
T7046	25390-25400	NNS	denotes	aggregates
T7047	25382-25389	NN	denotes	amyloid
T7048	25401-25405	WDT	denotes	that
T7049	25412-25415	VB	denotes	act
T7050	25406-25411	MD	denotes	might
T7051	25416-25418	IN	denotes	as
T7052	25419-25421	DT	denotes	an
T7053	25434-25443	NN	denotes	reservoir
T7054	25422-25433	JJ	denotes	intractable
T7055	25444-25446	IN	denotes	of
T7056	25447-25454	NN	denotes	peptide
T7057	25455-25464	VBG	denotes	remaining
T7058	25465-25467	IN	denotes	in
T7059	25468-25471	DT	denotes	the
T7060	25472-25477	NN	denotes	brain
T7061	25478-25483	IN	denotes	after
T7062	25507-25517	VBN	denotes	suppressed
T7063	25484-25487	DT	denotes	the
T7064	25488-25497	NN	denotes	transgene
T7065	25498-25501	VBD	denotes	had
T7066	25502-25506	VBN	denotes	been
T7067	25517-25518	.	denotes	.
T7068	25518-25764	sentence	denotes	To further ensure we could detect any such insoluble aggregates that might bias our measure of changes in peptide synthesis, we performed a sequential three-step extraction with PBS, 2% SDS, and 70% FA that would separate peptides by solubility.
T7069	25519-25521	TO	denotes	To
T7070	25530-25536	VB	denotes	ensure
T7071	25522-25529	RB	denotes	further
T7072	25647-25656	VBD	denotes	performed
T7073	25537-25539	PRP	denotes	we
T7074	25546-25552	VB	denotes	detect
T7075	25540-25545	MD	denotes	could
T7076	25553-25556	DT	denotes	any
T7077	25572-25582	NNS	denotes	aggregates
T7078	25557-25561	JJ	denotes	such
T7079	25562-25571	JJ	denotes	insoluble
T7080	25583-25587	WDT	denotes	that
T7081	25594-25598	VB	denotes	bias
T7082	25588-25593	MD	denotes	might
T7083	25599-25602	PRP$	denotes	our
T7084	25603-25610	NN	denotes	measure
T7085	25611-25613	IN	denotes	of
T7086	25614-25621	NNS	denotes	changes
T7087	25622-25624	IN	denotes	in
T7088	25625-25632	NN	denotes	peptide
T7089	25633-25642	NN	denotes	synthesis
T7090	25642-25644	,	denotes	,
T7091	25644-25646	PRP	denotes	we
T7092	25657-25658	DT	denotes	a
T7093	25681-25691	NN	denotes	extraction
T7094	25659-25669	JJ	denotes	sequential
T7095	25670-25675	CD	denotes	three
T7096	25676-25680	NN	denotes	step
T7097	25675-25676	HYPH	denotes	-
T7098	25692-25696	IN	denotes	with
T7099	25697-25700	NN	denotes	PBS
T7100	25700-25702	,	denotes	,
T7101	25702-25703	CD	denotes	2
T7102	25703-25704	NN	denotes	%
T7103	25705-25708	NN	denotes	SDS
T7104	25708-25710	,	denotes	,
T7105	25710-25713	CC	denotes	and
T7106	25714-25716	CD	denotes	70
T7107	25716-25717	NN	denotes	%
T7108	25718-25720	NN	denotes	FA
T7109	25721-25725	WDT	denotes	that
T7110	25732-25740	VB	denotes	separate
T7111	25726-25731	MD	denotes	would
T7112	25741-25749	NNS	denotes	peptides
T7113	25750-25752	IN	denotes	by
T7114	25753-25763	NN	denotes	solubility
T7115	25763-25764	.	denotes	.
T7116	25764-26107	sentence	denotes	We compared the levels of human transgene-derived Aβ40 and Aβ42 in untreated mice at 4 and 6 wk of age to animals that had either been born and raised on dox or that had been left untreated for 4 wk and then placed on dox chow for 2 wk prior to harvest (the same groups described above for immunoblot analysis of APPswe/ind levels, line 107).
T7117	25765-25767	PRP	denotes	We
T7118	25768-25776	VBD	denotes	compared
T7119	25777-25780	DT	denotes	the
T7120	25781-25787	NNS	denotes	levels
T7121	25788-25790	IN	denotes	of
T7122	25791-25796	JJ	denotes	human
T7123	25797-25806	NN	denotes	transgene
T7124	25807-25814	VBN	denotes	derived
T7125	25806-25807	HYPH	denotes	-
T7126	25815-25819	NN	denotes	Aβ40
T7127	25820-25823	CC	denotes	and
T7128	25824-25828	NN	denotes	Aβ42
T7129	25829-25831	IN	denotes	in
T7130	25832-25841	JJ	denotes	untreated
T7131	25842-25846	NNS	denotes	mice
T7132	25847-25849	IN	denotes	at
T7133	25850-25851	CD	denotes	4
T7134	25858-25860	NN	denotes	wk
T7135	25852-25855	CC	denotes	and
T7136	25856-25857	CD	denotes	6
T7137	25861-25863	IN	denotes	of
T7138	25864-25867	NN	denotes	age
T7139	25868-25870	IN	denotes	to
T7140	25871-25878	NNS	denotes	animals
T7141	25879-25883	WDT	denotes	that
T7142	25900-25904	VBN	denotes	born
T7143	25884-25887	VBD	denotes	had
T7144	25888-25894	CC	denotes	either
T7145	25895-25899	VBN	denotes	been
T7146	25905-25908	CC	denotes	and
T7147	25909-25915	VBN	denotes	raised
T7148	25916-25918	IN	denotes	on
T7149	25919-25922	NN	denotes	dox
T7150	25923-25925	CC	denotes	or
T7151	25926-25930	WDT	denotes	that
T7152	25940-25944	VBN	denotes	left
T7153	25931-25934	VBD	denotes	had
T7154	25935-25939	VBN	denotes	been
T7155	25945-25954	JJ	denotes	untreated
T7156	25955-25958	IN	denotes	for
T7157	25959-25960	CD	denotes	4
T7158	25961-25963	NN	denotes	wk
T7159	25964-25967	CC	denotes	and
T7160	25968-25972	RB	denotes	then
T7161	25973-25979	VBN	denotes	placed
T7162	25980-25982	IN	denotes	on
T7163	25983-25986	NN	denotes	dox
T7164	25987-25991	NN	denotes	chow
T7165	25992-25995	IN	denotes	for
T7166	25996-25997	CD	denotes	2
T7167	25998-26000	NN	denotes	wk
T7168	26001-26006	JJ	denotes	prior
T7169	26007-26009	IN	denotes	to
T7170	26010-26017	NN	denotes	harvest
T7171	26018-26019	-LRB-	denotes	(
T7172	26097-26101	NN	denotes	line
T7173	26019-26022	DT	denotes	the
T7174	26028-26034	NNS	denotes	groups
T7175	26023-26027	JJ	denotes	same
T7176	26035-26044	VBN	denotes	described
T7177	26045-26050	RB	denotes	above
T7178	26051-26054	IN	denotes	for
T7179	26055-26065	NN	denotes	immunoblot
T7180	26066-26074	NN	denotes	analysis
T7181	26075-26077	IN	denotes	of
T7182	26078-26084	NN	denotes	APPswe
T7183	26085-26088	NN	denotes	ind
T7184	26084-26085	HYPH	denotes	/
T7185	26089-26095	NNS	denotes	levels
T7186	26095-26097	,	denotes	,
T7187	26102-26105	CD	denotes	107
T7188	26105-26106	-RRB-	denotes	)
T7189	26106-26107	.	denotes	.
T7190	26107-26383	sentence	denotes	Consistent with the reduction in full-length APPswe/ind synthesis shown by immunoblot (see Figure 1), we found that transgene-derived Aβ levels were completely suppressed in animals born and raised on dox, and were sharply reduced following acute (2 wk) antibiotic treatment.
T7191	26108-26118	JJ	denotes	Consistent
T7192	26213-26218	VBD	denotes	found
T7193	26119-26123	IN	denotes	with
T7194	26124-26127	DT	denotes	the
T7195	26128-26137	NN	denotes	reduction
T7196	26138-26140	IN	denotes	in
T7197	26141-26145	JJ	denotes	full
T7198	26146-26152	NN	denotes	length
T7199	26145-26146	HYPH	denotes	-
T7200	26164-26173	NN	denotes	synthesis
T7201	26153-26159	NN	denotes	APPswe
T7202	26160-26163	NN	denotes	ind
T7203	26159-26160	HYPH	denotes	/
T7204	26174-26179	VBN	denotes	shown
T7205	26180-26182	IN	denotes	by
T7206	26183-26193	NN	denotes	immunoblot
T7207	26194-26195	-LRB-	denotes	(
T7208	26195-26198	VB	denotes	see
T7209	26199-26205	NN	denotes	Figure
T7210	26206-26207	CD	denotes	1
T7211	26207-26208	-RRB-	denotes	)
T7212	26208-26210	,	denotes	,
T7213	26210-26212	PRP	denotes	we
T7214	26219-26223	IN	denotes	that
T7215	26268-26278	VBN	denotes	suppressed
T7216	26224-26233	NN	denotes	transgene
T7217	26234-26241	VBN	denotes	derived
T7218	26233-26234	HYPH	denotes	-
T7219	26245-26251	NNS	denotes	levels
T7220	26242-26244	NN	denotes	Aβ
T7221	26252-26256	VBD	denotes	were
T7222	26257-26267	RB	denotes	completely
T7223	26279-26281	IN	denotes	in
T7224	26282-26289	NNS	denotes	animals
T7225	26290-26294	VBN	denotes	born
T7226	26295-26298	CC	denotes	and
T7227	26299-26305	VBN	denotes	raised
T7228	26306-26308	IN	denotes	on
T7229	26309-26312	NN	denotes	dox
T7230	26312-26314	,	denotes	,
T7231	26314-26317	CC	denotes	and
T7232	26318-26322	VBD	denotes	were
T7233	26331-26338	VBN	denotes	reduced
T7234	26323-26330	RB	denotes	sharply
T7235	26339-26348	VBG	denotes	following
T7236	26349-26354	JJ	denotes	acute
T7237	26373-26382	NN	denotes	treatment
T7238	26355-26356	-LRB-	denotes	(
T7239	26358-26360	NN	denotes	wk
T7240	26356-26357	CD	denotes	2
T7241	26360-26361	-RRB-	denotes	)
T7242	26362-26372	JJ	denotes	antibiotic
T7243	26382-26383	.	denotes	.
T7244	26383-26549	sentence	denotes	Compared to the levels in untreated 4-wk-old mice, PBS-soluble Aβ42 dropped by 95.2% following 2 wk of dox treatment and by 99.2% with chronic treatment (Figure 2A).
T7245	26384-26392	VBN	denotes	Compared
T7246	26452-26459	VBD	denotes	dropped
T7247	26393-26395	IN	denotes	to
T7248	26396-26399	DT	denotes	the
T7249	26400-26406	NNS	denotes	levels
T7250	26407-26409	IN	denotes	in
T7251	26410-26419	JJ	denotes	untreated
T7252	26429-26433	NNS	denotes	mice
T7253	26420-26421	CD	denotes	4
T7254	26422-26424	NN	denotes	wk
T7255	26421-26422	HYPH	denotes	-
T7256	26425-26428	JJ	denotes	old
T7257	26424-26425	HYPH	denotes	-
T7258	26433-26435	,	denotes	,
T7259	26435-26438	NN	denotes	PBS
T7260	26439-26446	JJ	denotes	soluble
T7261	26438-26439	HYPH	denotes	-
T7262	26447-26451	NN	denotes	Aβ42
T7263	26460-26462	IN	denotes	by
T7264	26463-26467	CD	denotes	95.2
T7265	26467-26468	NN	denotes	%
T7266	26469-26478	VBG	denotes	following
T7267	26479-26480	CD	denotes	2
T7268	26481-26483	NN	denotes	wk
T7269	26484-26486	IN	denotes	of
T7270	26487-26490	NN	denotes	dox
T7271	26491-26500	NN	denotes	treatment
T7272	26501-26504	CC	denotes	and
T7273	26505-26507	IN	denotes	by
T7274	26514-26518	IN	denotes	with
T7275	26508-26512	CD	denotes	99.2
T7276	26512-26513	NN	denotes	%
T7277	26519-26526	JJ	denotes	chronic
T7278	26527-26536	NN	denotes	treatment
T7279	26537-26538	-LRB-	denotes	(
T7280	26545-26547	NN	denotes	2A
T7281	26538-26544	NN	denotes	Figure
T7282	26547-26548	-RRB-	denotes	)
T7283	26548-26549	.	denotes	.
T7284	26549-26660	sentence	denotes	Similarly, SDS-soluble Aβ42 decreased by 75.2% and 94.8% following 2-wk or lifelong dox treatment (Figure 2B).
T7285	26550-26559	RB	denotes	Similarly
T7286	26578-26587	VBD	denotes	decreased
T7287	26559-26561	,	denotes	,
T7288	26561-26564	NN	denotes	SDS
T7289	26565-26572	JJ	denotes	soluble
T7290	26564-26565	HYPH	denotes	-
T7291	26573-26577	NN	denotes	Aβ42
T7292	26588-26590	IN	denotes	by
T7293	26591-26595	CD	denotes	75.2
T7294	26595-26596	NN	denotes	%
T7295	26597-26600	CC	denotes	and
T7296	26601-26605	CD	denotes	94.8
T7297	26605-26606	NN	denotes	%
T7298	26607-26616	VBG	denotes	following
T7299	26617-26618	CD	denotes	2
T7300	26619-26621	NN	denotes	wk
T7301	26618-26619	HYPH	denotes	-
T7302	26638-26647	NN	denotes	treatment
T7303	26622-26624	CC	denotes	or
T7304	26625-26633	JJ	denotes	lifelong
T7305	26634-26637	NN	denotes	dox
T7306	26648-26649	-LRB-	denotes	(
T7307	26656-26658	NN	denotes	2B
T7308	26649-26655	NN	denotes	Figure
T7309	26658-26659	-RRB-	denotes	)
T7310	26659-26660	.	denotes	.
T7311	26660-26894	sentence	denotes	Only the FA fraction revealed a small dox-resistant pool of peptide in acutely treated animals that we believe represents stable predeposit aggregates that have already accumulated by 4 wk of age when treatment was begun (Figure 2C).
T7312	26661-26665	RB	denotes	Only
T7313	26673-26681	NN	denotes	fraction
T7314	26666-26669	DT	denotes	the
T7315	26670-26672	NN	denotes	FA
T7316	26682-26690	VBD	denotes	revealed
T7317	26691-26692	DT	denotes	a
T7318	26713-26717	NN	denotes	pool
T7319	26693-26698	JJ	denotes	small
T7320	26699-26702	NN	denotes	dox
T7321	26703-26712	JJ	denotes	resistant
T7322	26702-26703	HYPH	denotes	-
T7323	26718-26720	IN	denotes	of
T7324	26721-26728	NN	denotes	peptide
T7325	26729-26731	IN	denotes	in
T7326	26732-26739	RB	denotes	acutely
T7327	26740-26747	VBN	denotes	treated
T7328	26748-26755	NNS	denotes	animals
T7329	26756-26760	WDT	denotes	that
T7330	26764-26771	VBP	denotes	believe
T7331	26761-26763	PRP	denotes	we
T7332	26772-26782	VBZ	denotes	represents
T7333	26783-26789	JJ	denotes	stable
T7334	26801-26811	NNS	denotes	aggregates
T7335	26790-26800	JJ	denotes	predeposit
T7336	26812-26816	WDT	denotes	that
T7337	26830-26841	VBN	denotes	accumulated
T7338	26817-26821	VBP	denotes	have
T7339	26822-26829	RB	denotes	already
T7340	26842-26844	IN	denotes	by
T7341	26845-26846	CD	denotes	4
T7342	26847-26849	NN	denotes	wk
T7343	26850-26852	IN	denotes	of
T7344	26853-26856	NN	denotes	age
T7345	26857-26861	WRB	denotes	when
T7346	26876-26881	VBN	denotes	begun
T7347	26862-26871	NN	denotes	treatment
T7348	26872-26875	VBD	denotes	was
T7349	26882-26883	-LRB-	denotes	(
T7350	26890-26892	NN	denotes	2C
T7351	26883-26889	NN	denotes	Figure
T7352	26892-26893	-RRB-	denotes	)
T7353	26893-26894	.	denotes	.
T7354	26894-27056	sentence	denotes	Indeed, animals that were born and raised on dox did not harbor this reservoir of treatment-resistant peptide, with 96.3% less Aβ42 than untreated 4-wk-old mice.
T7355	26895-26901	RB	denotes	Indeed
T7356	26952-26958	VB	denotes	harbor
T7357	26901-26903	,	denotes	,
T7358	26903-26910	NNS	denotes	animals
T7359	26911-26915	WDT	denotes	that
T7360	26921-26925	VBN	denotes	born
T7361	26916-26920	VBD	denotes	were
T7362	26926-26929	CC	denotes	and
T7363	26930-26936	VBN	denotes	raised
T7364	26937-26939	IN	denotes	on
T7365	26940-26943	NN	denotes	dox
T7366	26944-26947	VBD	denotes	did
T7367	26948-26951	RB	denotes	not
T7368	26959-26963	DT	denotes	this
T7369	26964-26973	NN	denotes	reservoir
T7370	26974-26976	IN	denotes	of
T7371	26977-26986	NN	denotes	treatment
T7372	26987-26996	JJ	denotes	resistant
T7373	26986-26987	HYPH	denotes	-
T7374	26997-27004	NN	denotes	peptide
T7375	27004-27006	,	denotes	,
T7376	27006-27010	IN	denotes	with
T7377	27011-27015	CD	denotes	96.3
T7378	27015-27016	NN	denotes	%
T7379	27017-27021	JJR	denotes	less
T7380	27022-27026	NN	denotes	Aβ42
T7381	27027-27031	IN	denotes	than
T7382	27032-27041	JJ	denotes	untreated
T7383	27051-27055	NNS	denotes	mice
T7384	27042-27043	CD	denotes	4
T7385	27044-27046	NN	denotes	wk
T7386	27043-27044	HYPH	denotes	-
T7387	27047-27050	JJ	denotes	old
T7388	27046-27047	HYPH	denotes	-
T7389	27055-27056	.	denotes	.
T7390	27056-27301	sentence	denotes	Measurement of total Aβ in chronically treated mice, including endogenous and transgene-derived peptide, demonstrated that Aβ levels in tet-off APP mice were reduced to the level of endogenous peptide found in nontransgenic animals (Figure 2D).
T7391	27057-27068	NN	denotes	Measurement
T7392	27162-27174	VBD	denotes	demonstrated
T7393	27069-27071	IN	denotes	of
T7394	27072-27077	JJ	denotes	total
T7395	27078-27080	NN	denotes	Aβ
T7396	27081-27083	IN	denotes	in
T7397	27084-27095	RB	denotes	chronically
T7398	27096-27103	VBN	denotes	treated
T7399	27104-27108	NNS	denotes	mice
T7400	27108-27110	,	denotes	,
T7401	27110-27119	VBG	denotes	including
T7402	27120-27130	JJ	denotes	endogenous
T7403	27153-27160	NN	denotes	peptide
T7404	27131-27134	CC	denotes	and
T7405	27135-27144	NN	denotes	transgene
T7406	27145-27152	VBN	denotes	derived
T7407	27144-27145	HYPH	denotes	-
T7408	27160-27162	,	denotes	,
T7409	27175-27179	IN	denotes	that
T7410	27215-27222	VBN	denotes	reduced
T7411	27180-27182	NN	denotes	Aβ
T7412	27183-27189	NNS	denotes	levels
T7413	27190-27192	IN	denotes	in
T7414	27193-27196	NN	denotes	tet
T7415	27197-27200	NN	denotes	off
T7416	27196-27197	HYPH	denotes	-
T7417	27205-27209	NNS	denotes	mice
T7418	27201-27204	NN	denotes	APP
T7419	27210-27214	VBD	denotes	were
T7420	27223-27225	IN	denotes	to
T7421	27226-27229	DT	denotes	the
T7422	27230-27235	NN	denotes	level
T7423	27236-27238	IN	denotes	of
T7424	27239-27249	JJ	denotes	endogenous
T7425	27250-27257	NN	denotes	peptide
T7426	27258-27263	VBN	denotes	found
T7427	27264-27266	IN	denotes	in
T7428	27267-27280	JJ	denotes	nontransgenic
T7429	27281-27288	NNS	denotes	animals
T7430	27289-27290	-LRB-	denotes	(
T7431	27297-27299	NN	denotes	2D
T7432	27290-27296	NN	denotes	Figure
T7433	27299-27300	-RRB-	denotes	)
T7434	27300-27301	.	denotes	.
T7435	27301-27512	sentence	denotes	Taken together with the immunoblotting data for full-length APPswe/ind, the ELISA measurements indicate that dox-mediated suppression of transgenic APPswe/ind synthesis leads to parallel reduction of Aβ levels.
T7436	27302-27307	VBN	denotes	Taken
T7437	27397-27405	VBP	denotes	indicate
T7438	27308-27316	RB	denotes	together
T7439	27317-27321	IN	denotes	with
T7440	27322-27325	DT	denotes	the
T7441	27341-27345	NNS	denotes	data
T7442	27326-27340	NN	denotes	immunoblotting
T7443	27346-27349	IN	denotes	for
T7444	27350-27354	JJ	denotes	full
T7445	27355-27361	NN	denotes	length
T7446	27354-27355	HYPH	denotes	-
T7447	27369-27372	NN	denotes	ind
T7448	27362-27368	NN	denotes	APPswe
T7449	27368-27369	HYPH	denotes	/
T7450	27372-27374	,	denotes	,
T7451	27374-27377	DT	denotes	the
T7452	27384-27396	NNS	denotes	measurements
T7453	27378-27383	NN	denotes	ELISA
T7454	27406-27410	IN	denotes	that
T7455	27471-27476	VBZ	denotes	leads
T7456	27411-27414	NN	denotes	dox
T7457	27415-27423	VBN	denotes	mediated
T7458	27414-27415	HYPH	denotes	-
T7459	27424-27435	NN	denotes	suppression
T7460	27436-27438	IN	denotes	of
T7461	27439-27449	JJ	denotes	transgenic
T7462	27461-27470	NN	denotes	synthesis
T7463	27450-27456	NN	denotes	APPswe
T7464	27457-27460	NN	denotes	ind
T7465	27456-27457	HYPH	denotes	/
T7466	27477-27479	IN	denotes	to
T7467	27480-27488	JJ	denotes	parallel
T7468	27489-27498	NN	denotes	reduction
T7469	27499-27501	IN	denotes	of
T7470	27502-27504	NN	denotes	Aβ
T7471	27505-27511	NNS	denotes	levels
T7472	27511-27512	.	denotes	.
T7473	27512-30499	sentence	denotes	Figure 2 Aβ Levels Are Dramatically Reduced by Transgene Suppression Cortical homogenates from young, predeposit tTA/APP mice used for Western blot in Figure 1 (line 107) were fractionated by sequential multi-step extraction with PBS, 2% SDS, and 70% FA followed by human-specific Aβ ELISA to measure transgene-derived peptide in each fraction. Aβ40 is shown in white, Aβ42 in black. (A) PBS-soluble Aβ levels are substantially reduced by both acute and chronic dox treatment (ANOVA, effect of treatment group F 4,24 = 137.10 and 386.01, p < 0.001, for Aβ40 and Aβ42, respectively). Aβ levels in treated animals are indistinguishable from nontransgenic (NTg) animals (p > 0.3, Tukey post-hoc test). (B) In the young animals tested here prior to the formation of visible amyloid deposits, most Aβ is extracted into the SDS fraction (84% and 76% of all transgene-derived Aβ40 and Aβ42, respectively). As in the PBS-soluble fraction, Aβ levels in the SDS fraction are significantly lowered by dox treatment compared to untreated animals (ANOVA effect of group F 4,24 = 197.57 and 163.48, p < 0.001, for Aβ40 and Aβ42, respectively). Acutely treated animals retained a small (although significant) amount of residual peptide (p < 0.001 compared to nontransgeinc, Tukey post-hoc test), whereas Aβ levels in mice born and raised on dox were reduced to levels indistinguishable from nontransgenic (p > 0.8, Tukey post-hoc test). (C) The FA-soluble fraction already contains a small but significant pool of aggregated Aβ42 in untreated animals by 4 wk of age (p < 0.05 versus nontransgenic; Tukey post-hoc test applied to significant effect of group ANOVA F 4,24 = 17.11, p < 0.001). By 6 wk of age, the amount of Aβ in the FA fraction is increased significantly preceding the appearance of visible deposits 2 wk later. The FA pool is the only peptide fraction not lowered by acute dox treatment (4 wk untreated = 4 wk + 2 wk dox, p > 0.9, Tukey post-hoc test), consistent with poor turnover of aggregated Aβ species. (D) Measurements of total Aβ, including both endogenous and transgene-derived peptides, show that animals born and raised on dox harbor Aβ levels identical to nontransgenic animals (p > 0.9, Tukey post-hoc test, effect of group ANOVA F 4,24 = 39.13 and 35.29, p < 0.001, for Aβ40 and Aβ42, respectively). Whereas chronic transgene suppression fully prevents synthesis of both peptides, acute dox treatment fully suppresses Aβ40 levels (p > 0.8, Tukey post-hoc test), but leaves a small amount of nonsuppressed Aβ42. The residual Aβ42 observed in acutely treated young animals derives from uncleared aggregates extracted in the SDS and FA fractions. , p < 0.05; , p < 0.005; **, p < 0.001 versus 4-wk-old untreated mice, Tukey post-hoc applied to significant effect of group ANOVA. The ELISA data also confirmed that incorporation of the Swedish and Indiana mutations led to high levels of Aβ42, which we predicted would induce rapid plaque formation in untreated animals.
T7474	30309-30312	DT	denotes	The
T7475	30319-30323	NNS	denotes	data
T7476	30313-30318	NN	denotes	ELISA
T7477	30329-30338	VBD	denotes	confirmed
T7478	30324-30328	RB	denotes	also
T7479	30339-30343	IN	denotes	that
T7480	30395-30398	VBD	denotes	led
T7481	30344-30357	NN	denotes	incorporation
T7482	30358-30360	IN	denotes	of
T7483	30361-30364	DT	denotes	the
T7484	30385-30394	NNS	denotes	mutations
T7485	30365-30372	JJ	denotes	Swedish
T7486	30373-30376	CC	denotes	and
T7487	30377-30384	NNP	denotes	Indiana
T7488	30399-30401	IN	denotes	to
T7489	30402-30406	JJ	denotes	high
T7490	30407-30413	NNS	denotes	levels
T7491	30414-30416	IN	denotes	of
T7492	30417-30421	NN	denotes	Aβ42
T7493	30421-30423	,	denotes	,
T7494	30423-30428	WDT	denotes	which
T7495	30432-30441	VBD	denotes	predicted
T7496	30429-30431	PRP	denotes	we
T7497	30442-30447	MD	denotes	would
T7498	30448-30454	VB	denotes	induce
T7499	30455-30460	JJ	denotes	rapid
T7500	30468-30477	NN	denotes	formation
T7501	30461-30467	NN	denotes	plaque
T7502	30478-30480	IN	denotes	in
T7503	30481-30490	JJ	denotes	untreated
T7504	30491-30498	NNS	denotes	animals
T7505	30498-30499	.	denotes	.
T7506	30499-30648	sentence	denotes	Histological characterization of double transgenic (CaMKIIα-tTA × tet-APPswe/ind) mice revealed early-onset amyloid formation in all four new lines.
T7507	30500-30512	JJ	denotes	Histological
T7508	30513-30529	NN	denotes	characterization
T7509	30587-30595	VBD	denotes	revealed
T7510	30530-30532	IN	denotes	of
T7511	30533-30539	JJ	denotes	double
T7512	30582-30586	NNS	denotes	mice
T7513	30540-30550	JJ	denotes	transgenic
T7514	30551-30552	-LRB-	denotes	(
T7515	30570-30576	NN	denotes	APPswe
T7516	30552-30559	NN	denotes	CaMKIIα
T7517	30559-30560	HYPH	denotes	-
T7518	30560-30563	NN	denotes	tTA
T7519	30564-30565	SYM	denotes	×
T7520	30566-30569	NN	denotes	tet
T7521	30569-30570	HYPH	denotes	-
T7522	30576-30577	HYPH	denotes	/
T7523	30577-30580	JJ	denotes	ind
T7524	30580-30581	-RRB-	denotes	)
T7525	30596-30601	JJ	denotes	early
T7526	30602-30607	NN	denotes	onset
T7527	30601-30602	HYPH	denotes	-
T7528	30616-30625	NN	denotes	formation
T7529	30608-30615	NN	denotes	amyloid
T7530	30626-30628	IN	denotes	in
T7531	30629-30632	DT	denotes	all
T7532	30642-30647	NNS	denotes	lines
T7533	30633-30637	CD	denotes	four
T7534	30638-30641	JJ	denotes	new
T7535	30647-30648	.	denotes	.
T7536	30648-30724	sentence	denotes	Amyloid plaques were seen in mice as young as 8 wk of age (data not shown).
T7537	30649-30656	NN	denotes	Amyloid
T7538	30657-30664	NNS	denotes	plaques
T7539	30670-30674	VBN	denotes	seen
T7540	30665-30669	VBD	denotes	were
T7541	30675-30677	IN	denotes	in
T7542	30678-30682	NNS	denotes	mice
T7543	30683-30685	RB	denotes	as
T7544	30686-30691	JJ	denotes	young
T7545	30692-30694	IN	denotes	as
T7546	30695-30696	CD	denotes	8
T7547	30697-30699	NN	denotes	wk
T7548	30700-30702	IN	denotes	of
T7549	30703-30706	NN	denotes	age
T7550	30707-30708	-LRB-	denotes	(
T7551	30717-30722	VBN	denotes	shown
T7552	30708-30712	NNS	denotes	data
T7553	30713-30716	RB	denotes	not
T7554	30722-30723	-RRB-	denotes	)
T7555	30723-30724	.	denotes	.
T7556	30724-30903	sentence	denotes	Plaques were limited to the forebrain, including the cortex, hippocampus, olfactory bulb, and striatum, where the CaMKIIα promoter is known to be most active [16,28] (Figure S2).
T7557	30725-30732	NNS	denotes	Plaques
T7558	30738-30745	VBN	denotes	limited
T7559	30733-30737	VBD	denotes	were
T7560	30746-30748	IN	denotes	to
T7561	30749-30752	DT	denotes	the
T7562	30753-30762	NN	denotes	forebrain
T7563	30762-30764	,	denotes	,
T7564	30764-30773	VBG	denotes	including
T7565	30774-30777	DT	denotes	the
T7566	30778-30784	NN	denotes	cortex
T7567	30784-30786	,	denotes	,
T7568	30786-30797	NN	denotes	hippocampus
T7569	30797-30799	,	denotes	,
T7570	30799-30808	JJ	denotes	olfactory
T7571	30809-30813	NN	denotes	bulb
T7572	30813-30815	,	denotes	,
T7573	30815-30818	CC	denotes	and
T7574	30819-30827	NN	denotes	striatum
T7575	30827-30829	,	denotes	,
T7576	30829-30834	WRB	denotes	where
T7577	30859-30864	VBN	denotes	known
T7578	30835-30838	DT	denotes	the
T7579	30847-30855	NN	denotes	promoter
T7580	30839-30846	NN	denotes	CaMKIIα
T7581	30856-30858	VBZ	denotes	is
T7582	30865-30867	TO	denotes	to
T7583	30868-30870	VB	denotes	be
T7584	30871-30875	RBS	denotes	most
T7585	30876-30882	JJ	denotes	active
T7586	30883-30884	-LRB-	denotes	[
T7587	30887-30889	CD	denotes	28
T7588	30884-30886	CD	denotes	16
T7589	30886-30887	,	denotes	,
T7590	30889-30890	-RRB-	denotes	]
T7591	30891-30892	-LRB-	denotes	(
T7592	30899-30901	NN	denotes	S2
T7593	30892-30898	NN	denotes	Figure
T7594	30901-30902	-RRB-	denotes	)
T7595	30902-30903	.	denotes	.
T7596	30903-31013	sentence	denotes	By 6 mo of age, amyloid burden became severe, covering large areas of the cortex and hippocampus (Figure S3).
T7597	30904-30906	IN	denotes	By
T7598	30935-30941	VBD	denotes	became
T7599	30907-30908	CD	denotes	6
T7600	30909-30911	NN	denotes	mo
T7601	30912-30914	IN	denotes	of
T7602	30915-30918	NN	denotes	age
T7603	30918-30920	,	denotes	,
T7604	30920-30927	NN	denotes	amyloid
T7605	30928-30934	NN	denotes	burden
T7606	30942-30948	JJ	denotes	severe
T7607	30948-30950	,	denotes	,
T7608	30950-30958	VBG	denotes	covering
T7609	30959-30964	JJ	denotes	large
T7610	30965-30970	NNS	denotes	areas
T7611	30971-30973	IN	denotes	of
T7612	30974-30977	DT	denotes	the
T7613	30978-30984	NN	denotes	cortex
T7614	30985-30988	CC	denotes	and
T7615	30989-31000	NN	denotes	hippocampus
T7616	31001-31002	-LRB-	denotes	(
T7617	31009-31011	NN	denotes	S3
T7618	31002-31008	NN	denotes	Figure
T7619	31011-31012	-RRB-	denotes	)
T7620	31012-31013	.	denotes	.
T7621	31013-31142	sentence	denotes	No lesions were seen in the cerebellum or brain stem even at late ages, consistent with CaMKIIα-controlled transgene expression.
T7622	31014-31016	DT	denotes	No
T7623	31017-31024	NNS	denotes	lesions
T7624	31030-31034	VBN	denotes	seen
T7625	31025-31029	VBD	denotes	were
T7626	31035-31037	IN	denotes	in
T7627	31038-31041	DT	denotes	the
T7628	31042-31052	NN	denotes	cerebellum
T7629	31053-31055	CC	denotes	or
T7630	31056-31061	NN	denotes	brain
T7631	31062-31066	NN	denotes	stem
T7632	31067-31071	RB	denotes	even
T7633	31072-31074	IN	denotes	at
T7634	31075-31079	JJ	denotes	late
T7635	31080-31084	NNS	denotes	ages
T7636	31084-31086	,	denotes	,
T7637	31086-31096	JJ	denotes	consistent
T7638	31097-31101	IN	denotes	with
T7639	31102-31109	NN	denotes	CaMKIIα
T7640	31110-31120	VBN	denotes	controlled
T7641	31109-31110	HYPH	denotes	-
T7642	31131-31141	NN	denotes	expression
T7643	31121-31130	NN	denotes	transgene
T7644	31141-31142	.	denotes	.
T7645	31142-31276	sentence	denotes	Unlike what is thought to occur in the human disease, the first visible plaques in the tet-off APP mice are fibrillar-cored deposits.
T7646	31143-31149	IN	denotes	Unlike
T7647	31247-31250	VBP	denotes	are
T7648	31150-31154	WP	denotes	what
T7649	31158-31165	VBN	denotes	thought
T7650	31155-31157	VBZ	denotes	is
T7651	31166-31168	TO	denotes	to
T7652	31169-31174	VB	denotes	occur
T7653	31175-31177	IN	denotes	in
T7654	31178-31181	DT	denotes	the
T7655	31188-31195	NN	denotes	disease
T7656	31182-31187	JJ	denotes	human
T7657	31195-31197	,	denotes	,
T7658	31197-31200	DT	denotes	the
T7659	31215-31222	NNS	denotes	plaques
T7660	31201-31206	JJ	denotes	first
T7661	31207-31214	JJ	denotes	visible
T7662	31223-31225	IN	denotes	in
T7663	31226-31229	DT	denotes	the
T7664	31242-31246	NNS	denotes	mice
T7665	31230-31233	NN	denotes	tet
T7666	31234-31237	NN	denotes	off
T7667	31233-31234	HYPH	denotes	-
T7668	31238-31241	NN	denotes	APP
T7669	31251-31260	JJ	denotes	fibrillar
T7670	31261-31266	VBN	denotes	cored
T7671	31260-31261	HYPH	denotes	-
T7672	31267-31275	NNS	denotes	deposits
T7673	31275-31276	.	denotes	.
T7674	31276-31411	sentence	denotes	We have noted the same early appearance of cored deposits in other lines of APP transgenic mice that harbor the Swedish mutation [27].
T7675	31277-31279	PRP	denotes	We
T7676	31285-31290	VBN	denotes	noted
T7677	31280-31284	VBP	denotes	have
T7678	31291-31294	DT	denotes	the
T7679	31306-31316	NN	denotes	appearance
T7680	31295-31299	JJ	denotes	same
T7681	31300-31305	JJ	denotes	early
T7682	31317-31319	IN	denotes	of
T7683	31320-31325	VBN	denotes	cored
T7684	31326-31334	NNS	denotes	deposits
T7685	31335-31337	IN	denotes	in
T7686	31338-31343	JJ	denotes	other
T7687	31344-31349	NNS	denotes	lines
T7688	31350-31352	IN	denotes	of
T7689	31353-31356	NN	denotes	APP
T7690	31368-31372	NNS	denotes	mice
T7691	31357-31367	JJ	denotes	transgenic
T7692	31373-31377	WDT	denotes	that
T7693	31378-31384	VBP	denotes	harbor
T7694	31385-31388	DT	denotes	the
T7695	31397-31405	NN	denotes	mutation
T7696	31389-31396	JJ	denotes	Swedish
T7697	31406-31407	-LRB-	denotes	[
T7698	31407-31409	CD	denotes	27
T7699	31409-31410	-RRB-	denotes	]
T7700	31410-31411	.	denotes	.
T7701	31411-31514	sentence	denotes	Diffuse plaques were apparent in 6-mo-old tTA/APP mice, and became relatively abundant by 9 mo of age.
T7702	31412-31419	NN	denotes	Diffuse
T7703	31420-31427	NNS	denotes	plaques
T7704	31428-31432	VBD	denotes	were
T7705	31433-31441	JJ	denotes	apparent
T7706	31442-31444	IN	denotes	in
T7707	31445-31446	CD	denotes	6
T7708	31447-31449	NN	denotes	mo
T7709	31446-31447	HYPH	denotes	-
T7710	31450-31453	JJ	denotes	old
T7711	31449-31450	HYPH	denotes	-
T7712	31462-31466	NNS	denotes	mice
T7713	31454-31457	NN	denotes	tTA
T7714	31458-31461	NN	denotes	APP
T7715	31457-31458	HYPH	denotes	/
T7716	31466-31468	,	denotes	,
T7717	31468-31471	CC	denotes	and
T7718	31472-31478	VBD	denotes	became
T7719	31479-31489	RB	denotes	relatively
T7720	31490-31498	JJ	denotes	abundant
T7721	31499-31501	IN	denotes	by
T7722	31502-31503	CD	denotes	9
T7723	31504-31506	NN	denotes	mo
T7724	31507-31509	IN	denotes	of
T7725	31510-31513	NN	denotes	age
T7726	31513-31514	.	denotes	.
T7727	31514-31668	sentence	denotes	At older ages (9–12 mo) amyloid deposits were visible in the thalamus, which has also been observed in mice expressing mutant APP via the Thy-1 promoter.
T7728	31515-31517	IN	denotes	At
T7729	31556-31560	VBD	denotes	were
T7730	31518-31523	JJR	denotes	older
T7731	31524-31528	NNS	denotes	ages
T7732	31529-31530	-LRB-	denotes	(
T7733	31530-31531	CD	denotes	9
T7734	31532-31534	CD	denotes	12
T7735	31531-31532	SYM	denotes	–
T7736	31535-31537	NN	denotes	mo
T7737	31537-31538	-RRB-	denotes	)
T7738	31539-31546	NN	denotes	amyloid
T7739	31547-31555	NNS	denotes	deposits
T7740	31561-31568	JJ	denotes	visible
T7741	31569-31571	IN	denotes	in
T7742	31572-31575	DT	denotes	the
T7743	31576-31584	NN	denotes	thalamus
T7744	31584-31586	,	denotes	,
T7745	31586-31591	WDT	denotes	which
T7746	31606-31614	VBN	denotes	observed
T7747	31592-31595	VBZ	denotes	has
T7748	31596-31600	RB	denotes	also
T7749	31601-31605	VBN	denotes	been
T7750	31615-31617	IN	denotes	in
T7751	31618-31622	NNS	denotes	mice
T7752	31623-31633	VBG	denotes	expressing
T7753	31634-31640	NN	denotes	mutant
T7754	31641-31644	NN	denotes	APP
T7755	31645-31648	IN	denotes	via
T7756	31649-31652	DT	denotes	the
T7757	31659-31667	NN	denotes	promoter
T7758	31653-31656	NN	denotes	Thy
T7759	31656-31657	HYPH	denotes	-
T7760	31657-31658	CD	denotes	1
T7761	31667-31668	.	denotes	.
T7762	31668-31826	sentence	denotes	The presence of amyloid pathology in this region has been attributed to axonal transport of APP/Aβ to the terminals of cortical neurons in the thalamus [29].
T7763	31669-31672	DT	denotes	The
T7764	31673-31681	NN	denotes	presence
T7765	31727-31737	VBN	denotes	attributed
T7766	31682-31684	IN	denotes	of
T7767	31685-31692	NN	denotes	amyloid
T7768	31693-31702	NN	denotes	pathology
T7769	31703-31705	IN	denotes	in
T7770	31706-31710	DT	denotes	this
T7771	31711-31717	NN	denotes	region
T7772	31718-31721	VBZ	denotes	has
T7773	31722-31726	VBN	denotes	been
T7774	31738-31740	IN	denotes	to
T7775	31741-31747	JJ	denotes	axonal
T7776	31748-31757	NN	denotes	transport
T7777	31758-31760	IN	denotes	of
T7778	31761-31764	NN	denotes	APP
T7779	31765-31767	NN	denotes	Aβ
T7780	31764-31765	HYPH	denotes	/
T7781	31768-31770	IN	denotes	to
T7782	31771-31774	DT	denotes	the
T7783	31775-31784	NNS	denotes	terminals
T7784	31785-31787	IN	denotes	of
T7785	31788-31796	JJ	denotes	cortical
T7786	31797-31804	NNS	denotes	neurons
T7787	31805-31807	IN	denotes	in
T7788	31808-31811	DT	denotes	the
T7789	31812-31820	NN	denotes	thalamus
T7790	31821-31822	-LRB-	denotes	[
T7791	31822-31824	CD	denotes	29
T7792	31824-31825	-RRB-	denotes	]
T7793	31825-31826	.	denotes	.
T7794	31826-31944	sentence	denotes	Most importantly, only double transgenic mice, expressing both the tTA and APP transgenes, developed amyloid lesions.
T7795	31827-31831	RBS	denotes	Most
T7796	31832-31843	RB	denotes	importantly
T7797	31918-31927	VBD	denotes	developed
T7798	31843-31845	,	denotes	,
T7799	31845-31849	RB	denotes	only
T7800	31868-31872	NNS	denotes	mice
T7801	31850-31856	JJ	denotes	double
T7802	31857-31867	JJ	denotes	transgenic
T7803	31872-31874	,	denotes	,
T7804	31874-31884	VBG	denotes	expressing
T7805	31885-31889	CC	denotes	both
T7806	31906-31916	NNS	denotes	transgenes
T7807	31890-31893	DT	denotes	the
T7808	31894-31897	NN	denotes	tTA
T7809	31898-31901	CC	denotes	and
T7810	31902-31905	NN	denotes	APP
T7811	31916-31918	,	denotes	,
T7812	31928-31935	NN	denotes	amyloid
T7813	31936-31943	NNS	denotes	lesions
T7814	31943-31944	.	denotes	.
T7815	31944-32027	sentence	denotes	Single transgenic mice up to 15 mo of age showed no sign of pathology (Figure S3).
T7816	31945-31951	JJ	denotes	Single
T7817	31952-31962	JJ	denotes	transgenic
T7818	31963-31967	NNS	denotes	mice
T7819	31987-31993	VBD	denotes	showed
T7820	31968-31970	IN	denotes	up
T7821	31974-31976	CD	denotes	15
T7822	31971-31973	IN	denotes	to
T7823	31977-31979	NN	denotes	mo
T7824	31980-31982	IN	denotes	of
T7825	31983-31986	NN	denotes	age
T7826	31994-31996	DT	denotes	no
T7827	31997-32001	NN	denotes	sign
T7828	32002-32004	IN	denotes	of
T7829	32005-32014	NN	denotes	pathology
T7830	32015-32016	-LRB-	denotes	(
T7831	32023-32025	NN	denotes	S3
T7832	32016-32022	NN	denotes	Figure
T7833	32025-32026	-RRB-	denotes	)
T7834	32026-32027	.	denotes	.
T7835	32027-32137	sentence	denotes	Similarly, amyloid pathology can be completely prevented in double transgenic animals born and raised on dox.
T7836	32028-32037	RB	denotes	Similarly
T7837	32075-32084	VBN	denotes	prevented
T7838	32037-32039	,	denotes	,
T7839	32039-32046	NN	denotes	amyloid
T7840	32047-32056	NN	denotes	pathology
T7841	32057-32060	MD	denotes	can
T7842	32061-32063	VB	denotes	be
T7843	32064-32074	RB	denotes	completely
T7844	32085-32087	IN	denotes	in
T7845	32088-32094	JJ	denotes	double
T7846	32095-32105	JJ	denotes	transgenic
T7847	32106-32113	NNS	denotes	animals
T7848	32114-32118	VBN	denotes	born
T7849	32119-32122	CC	denotes	and
T7850	32123-32129	VBN	denotes	raised
T7851	32130-32132	IN	denotes	on
T7852	32133-32136	NN	denotes	dox
T7853	32136-32137	.	denotes	.
T7854	32137-32440	sentence	denotes	Animals from our highest expressing line (line 885) maintained on dox for up to 1 y harbored no amyloid pathology (data not shown), indicating that residual leakage of transgene expression in the presence of dox does not provide sufficient Aβ peptide to induce amyloid formation even over long periods.
T7855	32138-32145	NNS	denotes	Animals
T7856	32222-32230	VBD	denotes	harbored
T7857	32146-32150	IN	denotes	from
T7858	32151-32154	PRP$	denotes	our
T7859	32174-32178	NN	denotes	line
T7860	32155-32162	JJS	denotes	highest
T7861	32163-32173	VBG	denotes	expressing
T7862	32179-32180	-LRB-	denotes	(
T7863	32180-32184	NN	denotes	line
T7864	32185-32188	CD	denotes	885
T7865	32188-32189	-RRB-	denotes	)
T7866	32190-32200	VBN	denotes	maintained
T7867	32201-32203	IN	denotes	on
T7868	32204-32207	NN	denotes	dox
T7869	32208-32211	IN	denotes	for
T7870	32212-32214	IN	denotes	up
T7871	32215-32217	IN	denotes	to
T7872	32218-32219	CD	denotes	1
T7873	32220-32221	NN	denotes	y
T7874	32231-32233	DT	denotes	no
T7875	32242-32251	NN	denotes	pathology
T7876	32234-32241	NN	denotes	amyloid
T7877	32252-32253	-LRB-	denotes	(
T7878	32262-32267	VBN	denotes	shown
T7879	32253-32257	NNS	denotes	data
T7880	32258-32261	RB	denotes	not
T7881	32267-32268	-RRB-	denotes	)
T7882	32268-32270	,	denotes	,
T7883	32270-32280	VBG	denotes	indicating
T7884	32281-32285	IN	denotes	that
T7885	32359-32366	VB	denotes	provide
T7886	32286-32294	JJ	denotes	residual
T7887	32295-32302	NN	denotes	leakage
T7888	32303-32305	IN	denotes	of
T7889	32306-32315	NN	denotes	transgene
T7890	32316-32326	NN	denotes	expression
T7891	32327-32329	IN	denotes	in
T7892	32330-32333	DT	denotes	the
T7893	32334-32342	NN	denotes	presence
T7894	32343-32345	IN	denotes	of
T7895	32346-32349	NN	denotes	dox
T7896	32350-32354	VBZ	denotes	does
T7897	32355-32358	RB	denotes	not
T7898	32367-32377	JJ	denotes	sufficient
T7899	32381-32388	NN	denotes	peptide
T7900	32378-32380	NN	denotes	Aβ
T7901	32389-32391	TO	denotes	to
T7902	32392-32398	VB	denotes	induce
T7903	32399-32406	NN	denotes	amyloid
T7904	32407-32416	NN	denotes	formation
T7905	32417-32421	RB	denotes	even
T7906	32422-32426	IN	denotes	over
T7907	32427-32431	JJ	denotes	long
T7908	32432-32439	NNS	denotes	periods
T7909	32439-32440	.	denotes	.
T7910	32440-32689	sentence	denotes	To mimic therapeutic intervention with inhibitors of Aβ production, we raised a group of 25 double transgenic mice (CaMKIIα-tTA × APP line 107) on normal food until 6 mo of age, when we knew amyloid formation was already well underway in the brain.
T7911	32441-32443	TO	denotes	To
T7912	32444-32449	VB	denotes	mimic
T7913	32512-32518	VBD	denotes	raised
T7914	32450-32461	JJ	denotes	therapeutic
T7915	32462-32474	NN	denotes	intervention
T7916	32475-32479	IN	denotes	with
T7917	32480-32490	NNS	denotes	inhibitors
T7918	32491-32493	IN	denotes	of
T7919	32494-32496	NN	denotes	Aβ
T7920	32497-32507	NN	denotes	production
T7921	32507-32509	,	denotes	,
T7922	32509-32511	PRP	denotes	we
T7923	32519-32520	DT	denotes	a
T7924	32521-32526	NN	denotes	group
T7925	32527-32529	IN	denotes	of
T7926	32530-32532	CD	denotes	25
T7927	32551-32555	NNS	denotes	mice
T7928	32533-32539	JJ	denotes	double
T7929	32540-32550	JJ	denotes	transgenic
T7930	32556-32557	-LRB-	denotes	(
T7931	32571-32574	NN	denotes	APP
T7932	32557-32564	NN	denotes	CaMKIIα
T7933	32564-32565	HYPH	denotes	-
T7934	32565-32568	NN	denotes	tTA
T7935	32569-32570	SYM	denotes	×
T7936	32575-32579	NN	denotes	line
T7937	32580-32583	CD	denotes	107
T7938	32583-32584	-RRB-	denotes	)
T7939	32585-32587	IN	denotes	on
T7940	32588-32594	JJ	denotes	normal
T7941	32595-32599	NN	denotes	food
T7942	32600-32605	IN	denotes	until
T7943	32606-32607	CD	denotes	6
T7944	32608-32610	NN	denotes	mo
T7945	32611-32613	IN	denotes	of
T7946	32614-32617	NN	denotes	age
T7947	32617-32619	,	denotes	,
T7948	32619-32623	WRB	denotes	when
T7949	32627-32631	VBD	denotes	knew
T7950	32624-32626	PRP	denotes	we
T7951	32632-32639	NN	denotes	amyloid
T7952	32640-32649	NN	denotes	formation
T7953	32650-32653	VBD	denotes	was
T7954	32654-32661	RB	denotes	already
T7955	32662-32666	RB	denotes	well
T7956	32667-32675	JJ	denotes	underway
T7957	32676-32678	IN	denotes	in
T7958	32679-32682	DT	denotes	the
T7959	32683-32688	NN	denotes	brain
T7960	32688-32689	.	denotes	.
T7961	32689-32834	sentence	denotes	At 6 mo, half of the animals were switched from normal chow to food containing dox at 200 mg/kg until they were sacrificed at 9 or 12 mo of age.
T7962	32690-32692	IN	denotes	At
T7963	32724-32732	VBN	denotes	switched
T7964	32693-32694	CD	denotes	6
T7965	32695-32697	NN	denotes	mo
T7966	32697-32699	,	denotes	,
T7967	32699-32703	NN	denotes	half
T7968	32704-32706	IN	denotes	of
T7969	32707-32710	DT	denotes	the
T7970	32711-32718	NNS	denotes	animals
T7971	32719-32723	VBD	denotes	were
T7972	32733-32737	IN	denotes	from
T7973	32738-32744	JJ	denotes	normal
T7974	32745-32749	NN	denotes	chow
T7975	32750-32752	IN	denotes	to
T7976	32753-32757	NN	denotes	food
T7977	32758-32768	VBG	denotes	containing
T7978	32769-32772	NN	denotes	dox
T7979	32773-32775	IN	denotes	at
T7980	32776-32779	CD	denotes	200
T7981	32780-32782	NN	denotes	mg
T7982	32782-32783	SYM	denotes	/
T7983	32783-32785	NN	denotes	kg
T7984	32786-32791	IN	denotes	until
T7985	32802-32812	VBN	denotes	sacrificed
T7986	32792-32796	PRP	denotes	they
T7987	32797-32801	VBD	denotes	were
T7988	32813-32815	IN	denotes	at
T7989	32816-32817	CD	denotes	9
T7990	32824-32826	NN	denotes	mo
T7991	32818-32820	CC	denotes	or
T7992	32821-32823	CD	denotes	12
T7993	32827-32829	IN	denotes	of
T7994	32830-32833	NN	denotes	age
T7995	32833-32834	.	denotes	.
T7996	32834-32904	sentence	denotes	The remaining control animals were kept on standard chow (untreated).
T7997	32835-32838	DT	denotes	The
T7998	32857-32864	NNS	denotes	animals
T7999	32839-32848	VBG	denotes	remaining
T8000	32849-32856	NN	denotes	control
T8001	32870-32874	VBN	denotes	kept
T8002	32865-32869	VBD	denotes	were
T8003	32875-32877	IN	denotes	on
T8004	32878-32886	JJ	denotes	standard
T8005	32887-32891	NN	denotes	chow
T8006	32892-32893	-LRB-	denotes	(
T8007	32893-32902	JJ	denotes	untreated
T8008	32902-32903	-RRB-	denotes	)
T8009	32903-32904	.	denotes	.
T8010	32904-33049	sentence	denotes	In all, four cohorts were created: 6 mo untreated (n = 7), 9 mo untreated (n = 5), 6 mo + 3 mo treated (n = 8), and 6 mo + 6 mo treated (n = 5).
T8011	32905-32907	IN	denotes	In
T8012	32931-32938	VBN	denotes	created
T8013	32908-32911	DT	denotes	all
T8014	32911-32913	,	denotes	,
T8015	32913-32917	CD	denotes	four
T8016	32918-32925	NNS	denotes	cohorts
T8017	32926-32930	VBD	denotes	were
T8018	32938-32940	:	denotes	:
T8019	32940-32941	CD	denotes	6
T8020	32942-32944	NN	denotes	mo
T8021	32945-32954	JJ	denotes	untreated
T8022	32955-32956	-LRB-	denotes	(
T8023	32960-32961	CD	denotes	7
T8024	32956-32957	NN	denotes	n
T8025	32958-32959	SYM	denotes	=
T8026	32961-32962	-RRB-	denotes	)
T8027	32962-32964	,	denotes	,
T8028	32964-32965	CD	denotes	9
T8029	32966-32968	NN	denotes	mo
T8030	32969-32978	JJ	denotes	untreated
T8031	32979-32980	-LRB-	denotes	(
T8032	32984-32985	CD	denotes	5
T8033	32980-32981	NN	denotes	n
T8034	32982-32983	SYM	denotes	=
T8035	32985-32986	-RRB-	denotes	)
T8036	32986-32988	,	denotes	,
T8037	32988-32989	CD	denotes	6
T8038	32990-32992	NN	denotes	mo
T8039	32993-32994	SYM	denotes	+
T8040	32995-32996	CD	denotes	3
T8041	32997-32999	NN	denotes	mo
T8042	33000-33007	VBN	denotes	treated
T8043	33008-33009	-LRB-	denotes	(
T8044	33013-33014	CD	denotes	8
T8045	33009-33010	NN	denotes	n
T8046	33011-33012	SYM	denotes	=
T8047	33014-33015	-RRB-	denotes	)
T8048	33015-33017	,	denotes	,
T8049	33017-33020	CC	denotes	and
T8050	33021-33022	CD	denotes	6
T8051	33023-33025	NN	denotes	mo
T8052	33026-33027	SYM	denotes	+
T8053	33028-33029	CD	denotes	6
T8054	33030-33032	NN	denotes	mo
T8055	33033-33040	VBN	denotes	treated
T8056	33041-33042	-LRB-	denotes	(
T8057	33046-33047	CD	denotes	5
T8058	33042-33043	NN	denotes	n
T8059	33044-33045	SYM	denotes	=
T8060	33047-33048	-RRB-	denotes	)
T8061	33048-33049	.	denotes	.
T8062	33049-33172	sentence	denotes	Full suppression (>95%) of transgenic APPswe/ind levels in the dox-treated animals was confirmed by immunoblot (Figure 3).
T8063	33050-33054	JJ	denotes	Full
T8064	33055-33066	NN	denotes	suppression
T8065	33137-33146	VBN	denotes	confirmed
T8066	33067-33068	-LRB-	denotes	(
T8067	33071-33072	NN	denotes	%
T8068	33068-33069	SYM	denotes	>
T8069	33069-33071	CD	denotes	95
T8070	33072-33073	-RRB-	denotes	)
T8071	33074-33076	IN	denotes	of
T8072	33077-33087	JJ	denotes	transgenic
T8073	33099-33105	NNS	denotes	levels
T8074	33088-33094	NN	denotes	APPswe
T8075	33095-33098	NN	denotes	ind
T8076	33094-33095	HYPH	denotes	/
T8077	33106-33108	IN	denotes	in
T8078	33109-33112	DT	denotes	the
T8079	33125-33132	NNS	denotes	animals
T8080	33113-33116	NN	denotes	dox
T8081	33117-33124	VBN	denotes	treated
T8082	33116-33117	HYPH	denotes	-
T8083	33133-33136	VBD	denotes	was
T8084	33147-33149	IN	denotes	by
T8085	33150-33160	NN	denotes	immunoblot
T8086	33161-33162	-LRB-	denotes	(
T8087	33162-33168	NN	denotes	Figure
T8088	33169-33170	CD	denotes	3
T8089	33170-33171	-RRB-	denotes	)
T8090	33171-33172	.	denotes	.
T8091	33172-33399	sentence	denotes	To ensure that the transgene could be suppressed as rapidly in 6-mo-old mice with fulminant pathology as it can in young, predeposit animals, we treated an additional set of 6-mo-old animals with dox for 1 wk prior to harvest.
T8092	33173-33175	TO	denotes	To
T8093	33176-33182	VB	denotes	ensure
T8094	33318-33325	VBD	denotes	treated
T8095	33183-33187	IN	denotes	that
T8096	33211-33221	VBN	denotes	suppressed
T8097	33188-33191	DT	denotes	the
T8098	33192-33201	NN	denotes	transgene
T8099	33202-33207	MD	denotes	could
T8100	33208-33210	VB	denotes	be
T8101	33222-33224	RB	denotes	as
T8102	33225-33232	RB	denotes	rapidly
T8103	33233-33235	IN	denotes	in
T8104	33236-33237	CD	denotes	6
T8105	33238-33240	NN	denotes	mo
T8106	33237-33238	HYPH	denotes	-
T8107	33241-33244	JJ	denotes	old
T8108	33240-33241	HYPH	denotes	-
T8109	33245-33249	NNS	denotes	mice
T8110	33250-33254	IN	denotes	with
T8111	33255-33264	JJ	denotes	fulminant
T8112	33265-33274	NN	denotes	pathology
T8113	33275-33277	IN	denotes	as
T8114	33281-33284	MD	denotes	can
T8115	33278-33280	PRP	denotes	it
T8116	33285-33287	IN	denotes	in
T8117	33288-33293	JJ	denotes	young
T8118	33306-33313	NNS	denotes	animals
T8119	33293-33295	,	denotes	,
T8120	33295-33305	JJ	denotes	predeposit
T8121	33313-33315	,	denotes	,
T8122	33315-33317	PRP	denotes	we
T8123	33326-33328	DT	denotes	an
T8124	33340-33343	NN	denotes	set
T8125	33329-33339	JJ	denotes	additional
T8126	33344-33346	IN	denotes	of
T8127	33347-33348	CD	denotes	6
T8128	33349-33351	NN	denotes	mo
T8129	33348-33349	HYPH	denotes	-
T8130	33352-33355	JJ	denotes	old
T8131	33351-33352	HYPH	denotes	-
T8132	33356-33363	NNS	denotes	animals
T8133	33364-33368	IN	denotes	with
T8134	33369-33372	NN	denotes	dox
T8135	33373-33376	IN	denotes	for
T8136	33377-33378	CD	denotes	1
T8137	33379-33381	NN	denotes	wk
T8138	33382-33387	JJ	denotes	prior
T8139	33388-33390	IN	denotes	to
T8140	33391-33398	NN	denotes	harvest
T8141	33398-33399	.	denotes	.
T8142	33399-33640	sentence	denotes	Importantly, both APPswe/ind and APP–C-terminal fragment levels were fully suppressed after only 1 wk of treatment, indicating that the in vivo half-life of APPswe/ind and its processed C-terminal fragments are relatively short (Figure 3D).
T8143	33400-33411	RB	denotes	Importantly
T8144	33475-33485	VBN	denotes	suppressed
T8145	33411-33413	,	denotes	,
T8146	33413-33417	CC	denotes	both
T8147	33425-33428	NN	denotes	ind
T8148	33418-33424	NN	denotes	APPswe
T8149	33424-33425	HYPH	denotes	/
T8150	33439-33447	JJ	denotes	terminal
T8151	33429-33432	CC	denotes	and
T8152	33433-33436	NN	denotes	APP
T8153	33437-33438	NN	denotes	C
T8154	33436-33437	HYPH	denotes	–
T8155	33438-33439	HYPH	denotes	-
T8156	33457-33463	NNS	denotes	levels
T8157	33448-33456	NN	denotes	fragment
T8158	33464-33468	VBD	denotes	were
T8159	33469-33474	RB	denotes	fully
T8160	33486-33491	IN	denotes	after
T8161	33492-33496	RB	denotes	only
T8162	33499-33501	NN	denotes	wk
T8163	33497-33498	CD	denotes	1
T8164	33502-33504	IN	denotes	of
T8165	33505-33514	NN	denotes	treatment
T8166	33514-33516	,	denotes	,
T8167	33516-33526	VBG	denotes	indicating
T8168	33527-33531	IN	denotes	that
T8169	33607-33610	VBP	denotes	are
T8170	33532-33535	DT	denotes	the
T8171	33549-33553	NN	denotes	life
T8172	33536-33538	FW	denotes	in
T8173	33539-33543	FW	denotes	vivo
T8174	33544-33548	NN	denotes	half
T8175	33548-33549	HYPH	denotes	-
T8176	33554-33556	IN	denotes	of
T8177	33557-33563	NN	denotes	APPswe
T8178	33564-33567	NN	denotes	ind
T8179	33563-33564	HYPH	denotes	/
T8180	33568-33571	CC	denotes	and
T8181	33572-33575	PRP$	denotes	its
T8182	33597-33606	NNS	denotes	fragments
T8183	33576-33585	VBN	denotes	processed
T8184	33586-33587	NN	denotes	C
T8185	33588-33596	JJ	denotes	terminal
T8186	33587-33588	HYPH	denotes	-
T8187	33611-33621	RB	denotes	relatively
T8188	33622-33627	JJ	denotes	short
T8189	33628-33629	-LRB-	denotes	(
T8190	33636-33638	NN	denotes	3D
T8191	33629-33635	NN	denotes	Figure
T8192	33638-33639	-RRB-	denotes	)
T8193	33639-33640	.	denotes	.
T8194	33640-35569	sentence	denotes	Figure 3 Robust Transgene Suppression in Older Mice with Preexisting Amyloid Pathology (A) Cortical homogenates from 6- to 12-mo-old animals used for pathology studies described below (line 107) were immunoblotted with human-specific antibody 6E10 to examine transgene suppression following 3 or 6 mo of dox treatment. The blot was co-immunostained for endogenous superoxide dismutase 1 (SOD1) as a control for loading. (B) Quantitation of signal intensity from the Western blot shown in (A). Transgenic APP levels are significantly suppressed following 3 or 6 mo of dox treatment (96.9% and 97.6%, respectively). *, p < 0.001 compared to 6-mo-old untreated animals, Tukey post-hoc test applied to significant effect of group ANOVA F 3,12 = 107.22, p < 0.001. These data demonstrate that strong transgene suppression is attained both before and after the onset of amyloid pathology (see Figure 1 for predeposit experiments). (C) Experimental design. To examine the effects of chronic Aβ suppression on amyloid pathology after the onset of deposition, we compared untreated controls harvested at 6 and 9 mo of age to animals placed on dox at 6 mo of age and harvested after 3 or 6 mo of treatment. (D) Dox treatment leads to rapid transgene suppression even in 6-mo-old tTA/APP mice. Immunostaining with 6E10 shows APPswe/ind levels are dramatically reduced in 6-mo-old mice treated for 1 wk with dox (upper panel). A separate blot was immunostained for APP C-terminal fragments with CT15 antibody to show that the precursors to Aβ cleavage are decreased in parallel with the full-length protein (middle panel). Costaining for superoxide dismutase 1 was used as an internal control for loading (lower panel, taken from bottom half of 6E10 blot). Tissue sections from each animal in the four treatment groups were stained for amyloid pathology by Hirano silver, Campbell-Switzer silver, thioflavin-S, and Aβ immunohistochemistry.
T8195	35387-35393	NN	denotes	Tissue
T8196	35394-35402	NNS	denotes	sections
T8197	35454-35461	VBN	denotes	stained
T8198	35403-35407	IN	denotes	from
T8199	35408-35412	DT	denotes	each
T8200	35413-35419	NN	denotes	animal
T8201	35420-35422	IN	denotes	in
T8202	35423-35426	DT	denotes	the
T8203	35442-35448	NNS	denotes	groups
T8204	35427-35431	CD	denotes	four
T8205	35432-35441	NN	denotes	treatment
T8206	35449-35453	VBD	denotes	were
T8207	35462-35465	IN	denotes	for
T8208	35466-35473	NN	denotes	amyloid
T8209	35474-35483	NN	denotes	pathology
T8210	35484-35486	IN	denotes	by
T8211	35487-35493	NNP	denotes	Hirano
T8212	35494-35500	NN	denotes	silver
T8213	35500-35502	,	denotes	,
T8214	35502-35510	NNP	denotes	Campbell
T8215	35511-35518	NNP	denotes	Switzer
T8216	35510-35511	HYPH	denotes	-
T8217	35519-35525	NN	denotes	silver
T8218	35525-35527	,	denotes	,
T8219	35527-35537	NN	denotes	thioflavin
T8220	35538-35539	NN	denotes	S
T8221	35537-35538	HYPH	denotes	-
T8222	35539-35541	,	denotes	,
T8223	35541-35544	CC	denotes	and
T8224	35545-35547	NN	denotes	Aβ
T8225	35548-35568	NN	denotes	immunohistochemistry
T8226	35568-35569	.	denotes	.
T8227	35569-35715	sentence	denotes	As expected, the 6 mo untreated cohort displayed moderate amyloid pathology, and the 9 mo untreated cohort progressed to a severe amyloid burden.
T8228	35570-35572	IN	denotes	As
T8229	35573-35581	VBN	denotes	expected
T8230	35609-35618	VBD	denotes	displayed
T8231	35581-35583	,	denotes	,
T8232	35583-35586	DT	denotes	the
T8233	35602-35608	NN	denotes	cohort
T8234	35587-35588	CD	denotes	6
T8235	35589-35591	NN	denotes	mo
T8236	35592-35601	JJ	denotes	untreated
T8237	35619-35627	JJ	denotes	moderate
T8238	35636-35645	NN	denotes	pathology
T8239	35628-35635	NN	denotes	amyloid
T8240	35645-35647	,	denotes	,
T8241	35647-35650	CC	denotes	and
T8242	35651-35654	DT	denotes	the
T8243	35670-35676	NN	denotes	cohort
T8244	35655-35656	CD	denotes	9
T8245	35657-35659	NN	denotes	mo
T8246	35660-35669	JJ	denotes	untreated
T8247	35677-35687	VBD	denotes	progressed
T8248	35688-35690	IN	denotes	to
T8249	35691-35692	DT	denotes	a
T8250	35708-35714	NN	denotes	burden
T8251	35693-35699	JJ	denotes	severe
T8252	35700-35707	NN	denotes	amyloid
T8253	35714-35715	.	denotes	.
T8254	35715-35988	sentence	denotes	In contrast, the extent of amyloid pathology in mice from the 6 mo + 3 mo treated or 6 mo + 6 mo treated cohorts closely resembled that of the 6 mo untreated cohort, despite the significant age difference between the treated and untreated groups (Figures 4 and Figure S3).
T8255	35716-35718	IN	denotes	In
T8256	35837-35846	VBD	denotes	resembled
T8257	35719-35727	NN	denotes	contrast
T8258	35727-35729	,	denotes	,
T8259	35729-35732	DT	denotes	the
T8260	35733-35739	NN	denotes	extent
T8261	35740-35742	IN	denotes	of
T8262	35743-35750	NN	denotes	amyloid
T8263	35751-35760	NN	denotes	pathology
T8264	35761-35763	IN	denotes	in
T8265	35764-35768	NNS	denotes	mice
T8266	35769-35773	IN	denotes	from
T8267	35774-35777	DT	denotes	the
T8268	35821-35828	NNS	denotes	cohorts
T8269	35778-35779	CD	denotes	6
T8270	35780-35782	NN	denotes	mo
T8271	35790-35797	VBN	denotes	treated
T8272	35783-35784	SYM	denotes	+
T8273	35785-35786	CD	denotes	3
T8274	35787-35789	NN	denotes	mo
T8275	35798-35800	CC	denotes	or
T8276	35801-35802	CD	denotes	6
T8277	35803-35805	NN	denotes	mo
T8278	35813-35820	VBN	denotes	treated
T8279	35806-35807	SYM	denotes	+
T8280	35808-35809	CD	denotes	6
T8281	35810-35812	NN	denotes	mo
T8282	35829-35836	RB	denotes	closely
T8283	35847-35851	DT	denotes	that
T8284	35852-35854	IN	denotes	of
T8285	35855-35858	DT	denotes	the
T8286	35874-35880	NN	denotes	cohort
T8287	35859-35860	CD	denotes	6
T8288	35861-35863	NN	denotes	mo
T8289	35864-35873	JJ	denotes	untreated
T8290	35880-35882	,	denotes	,
T8291	35882-35889	IN	denotes	despite
T8292	35890-35893	DT	denotes	the
T8293	35910-35920	NN	denotes	difference
T8294	35894-35905	JJ	denotes	significant
T8295	35906-35909	NN	denotes	age
T8296	35921-35928	IN	denotes	between
T8297	35929-35932	DT	denotes	the
T8298	35955-35961	NNS	denotes	groups
T8299	35933-35940	VBN	denotes	treated
T8300	35941-35944	CC	denotes	and
T8301	35945-35954	JJ	denotes	untreated
T8302	35962-35963	-LRB-	denotes	(
T8303	35963-35970	NNS	denotes	Figures
T8304	35971-35972	CD	denotes	4
T8305	35973-35976	CC	denotes	and
T8306	35977-35983	NN	denotes	Figure
T8307	35984-35986	NN	denotes	S3
T8308	35986-35987	-RRB-	denotes	)
T8309	35987-35988	.	denotes	.
T8310	35988-36160	sentence	denotes	Well-formed plaques remained in the treated animals after 6 mo of transgene suppression, even though as much time was given to clear the lesions as they had taken to form.
T8311	35989-35993	RB	denotes	Well
T8312	35994-36000	VBN	denotes	formed
T8313	35993-35994	HYPH	denotes	-
T8314	36001-36008	NNS	denotes	plaques
T8315	36009-36017	VBD	denotes	remained
T8316	36018-36020	IN	denotes	in
T8317	36021-36024	DT	denotes	the
T8318	36033-36040	NNS	denotes	animals
T8319	36025-36032	VBN	denotes	treated
T8320	36041-36046	IN	denotes	after
T8321	36047-36048	CD	denotes	6
T8322	36049-36051	NN	denotes	mo
T8323	36052-36054	IN	denotes	of
T8324	36055-36064	NN	denotes	transgene
T8325	36065-36076	NN	denotes	suppression
T8326	36076-36078	,	denotes	,
T8327	36078-36082	RB	denotes	even
T8328	36107-36112	VBN	denotes	given
T8329	36083-36089	IN	denotes	though
T8330	36090-36092	RB	denotes	as
T8331	36093-36097	JJ	denotes	much
T8332	36098-36102	NN	denotes	time
T8333	36103-36106	VBD	denotes	was
T8334	36113-36115	TO	denotes	to
T8335	36116-36121	VB	denotes	clear
T8336	36122-36125	DT	denotes	the
T8337	36126-36133	NNS	denotes	lesions
T8338	36134-36136	IN	denotes	as
T8339	36146-36151	VBN	denotes	taken
T8340	36137-36141	PRP	denotes	they
T8341	36142-36145	VBD	denotes	had
T8342	36152-36154	TO	denotes	to
T8343	36155-36159	VB	denotes	form
T8344	36159-36160	.	denotes	.
T8345	36160-36254	sentence	denotes	Moreover, both types of amyloid, diffuse and fibrillar, remained intact throughout treatment.
T8346	36161-36169	RB	denotes	Moreover
T8347	36217-36225	VBD	denotes	remained
T8348	36169-36171	,	denotes	,
T8349	36171-36175	DT	denotes	both
T8350	36176-36181	NNS	denotes	types
T8351	36182-36184	IN	denotes	of
T8352	36185-36192	NN	denotes	amyloid
T8353	36192-36194	,	denotes	,
T8354	36194-36201	JJ	denotes	diffuse
T8355	36202-36205	CC	denotes	and
T8356	36206-36215	JJ	denotes	fibrillar
T8357	36215-36217	,	denotes	,
T8358	36226-36232	JJ	denotes	intact
T8359	36233-36243	IN	denotes	throughout
T8360	36244-36253	NN	denotes	treatment
T8361	36253-36254	.	denotes	.
T8362	36254-36412	sentence	denotes	Using the Campbell-Switzer silver stain to distinguish different forms of amyloid, we found diffuse plaques were as persistent as cored deposits (Figure S4).
T8363	36255-36260	VBG	denotes	Using
T8364	36341-36346	VBD	denotes	found
T8365	36261-36264	DT	denotes	the
T8366	36289-36294	NN	denotes	stain
T8367	36265-36273	NNP	denotes	Campbell
T8368	36274-36281	NNP	denotes	Switzer
T8369	36273-36274	HYPH	denotes	-
T8370	36282-36288	NN	denotes	silver
T8371	36295-36297	TO	denotes	to
T8372	36298-36309	VB	denotes	distinguish
T8373	36310-36319	JJ	denotes	different
T8374	36320-36325	NNS	denotes	forms
T8375	36326-36328	IN	denotes	of
T8376	36329-36336	NN	denotes	amyloid
T8377	36336-36338	,	denotes	,
T8378	36338-36340	PRP	denotes	we
T8379	36347-36354	JJ	denotes	diffuse
T8380	36355-36362	NNS	denotes	plaques
T8381	36363-36367	VBD	denotes	were
T8382	36368-36370	RB	denotes	as
T8383	36371-36381	JJ	denotes	persistent
T8384	36382-36384	IN	denotes	as
T8385	36385-36390	VBN	denotes	cored
T8386	36391-36399	NNS	denotes	deposits
T8387	36400-36401	-LRB-	denotes	(
T8388	36408-36410	NN	denotes	S4
T8389	36401-36407	NN	denotes	Figure
T8390	36410-36411	-RRB-	denotes	)
T8391	36411-36412	.	denotes	.
T8392	36412-36537	sentence	denotes	It was nevertheless clear that dox-induced suppression of transgenic APP had completely halted the progression of pathology.
T8393	36413-36415	PRP	denotes	It
T8394	36416-36419	VBD	denotes	was
T8395	36420-36432	RB	denotes	nevertheless
T8396	36433-36438	JJ	denotes	clear
T8397	36439-36443	IN	denotes	that
T8398	36501-36507	VBN	denotes	halted
T8399	36444-36447	NN	denotes	dox
T8400	36448-36455	VBN	denotes	induced
T8401	36447-36448	HYPH	denotes	-
T8402	36456-36467	NN	denotes	suppression
T8403	36468-36470	IN	denotes	of
T8404	36471-36481	JJ	denotes	transgenic
T8405	36482-36485	NN	denotes	APP
T8406	36486-36489	VBD	denotes	had
T8407	36490-36500	RB	denotes	completely
T8408	36508-36511	DT	denotes	the
T8409	36512-36523	NN	denotes	progression
T8410	36524-36526	IN	denotes	of
T8411	36527-36536	NN	denotes	pathology
T8412	36536-36537	.	denotes	.
T8413	36537-38441	sentence	denotes	Figure 4 Suppression of Transgenic APP Arrests Progression of Amyloid Pathology (A) Aggregated Aβ was quantified in cortical tissue from dox-treated and control tTA/APP mice (line 107) using a filter trap assay. Serial dilutions of protein homogenate were passed through a cellulose acetate filter; protein aggregates larger than the pore size were trapped and immunostained for Aβ. (B) Quantitation of signal intensity in the linear range of each filter trap dilution series (arrow in [A]) was used to compare aggregate load across treatment groups. Aggregated Aβ increased significantly between 6 and 9 mo of age in untreated mice (significant effect of group ANOVA F 3,18 = 7.85, p < 0.002). This progression of pathology was completely prevented by transgene suppression. The amount of aggregated Aβ was identical in untreated mice at 6 mo of age to that in 9- or 12-mo-old animals treated with dox (p > 0.9, Tukey post-hoc test). Single transgenic tTA samples were included as negative controls and showed no signal above background. , p < 0.01; , p < 0.005 versus 9-mo-old untreated mice, Tukey post-hoc test; **, p < 0.001 versus 9-mo-old untreated mice, Student's t-test. (C) Amyloid pathology in the hippocampus of representative mice from each treatment group: Hirano silver stain (top row), thioflavin-S (middle row), and Aβ immunohistochemistry (bottom row). Amyloid burden increases dramatically between 6 and 9 mo of age in untreated animals, but remains stable in transgene-suppressed mice over the same period (6 mo + 3 mo dox and 6 mo + 6 mo dox). Single transgenic animals (tTA only shown here) show no sign of amyloid pathology at any age tested. To confirm that the arrest of plaques without any sign of clearance was not unique to the line 107 mice, we repeated the dox-suppression experiment in a second line of tet-off APP mice (CaMKIIα-tTA × tet-APPswe/ind line 18; n = 22).
T8414	38209-38211	TO	denotes	To
T8415	38212-38219	VB	denotes	confirm
T8416	38317-38325	VBD	denotes	repeated
T8417	38220-38224	IN	denotes	that
T8418	38277-38280	VBD	denotes	was
T8419	38225-38228	DT	denotes	the
T8420	38229-38235	NN	denotes	arrest
T8421	38236-38238	IN	denotes	of
T8422	38239-38246	NNS	denotes	plaques
T8423	38247-38254	IN	denotes	without
T8424	38255-38258	DT	denotes	any
T8425	38259-38263	NN	denotes	sign
T8426	38264-38266	IN	denotes	of
T8427	38267-38276	NN	denotes	clearance
T8428	38281-38284	RB	denotes	not
T8429	38285-38291	JJ	denotes	unique
T8430	38292-38294	IN	denotes	to
T8431	38295-38298	DT	denotes	the
T8432	38308-38312	NNS	denotes	mice
T8433	38299-38303	NN	denotes	line
T8434	38304-38307	CD	denotes	107
T8435	38312-38314	,	denotes	,
T8436	38314-38316	PRP	denotes	we
T8437	38326-38329	DT	denotes	the
T8438	38346-38356	NN	denotes	experiment
T8439	38330-38333	NN	denotes	dox
T8440	38334-38345	NN	denotes	suppression
T8441	38333-38334	HYPH	denotes	-
T8442	38357-38359	IN	denotes	in
T8443	38360-38361	DT	denotes	a
T8444	38369-38373	NN	denotes	line
T8445	38362-38368	JJ	denotes	second
T8446	38374-38376	IN	denotes	of
T8447	38377-38380	NN	denotes	tet
T8448	38381-38384	NN	denotes	off
T8449	38380-38381	HYPH	denotes	-
T8450	38389-38393	NNS	denotes	mice
T8451	38385-38388	NN	denotes	APP
T8452	38394-38395	-LRB-	denotes	(
T8453	38424-38428	NN	denotes	line
T8454	38395-38402	NN	denotes	CaMKIIα
T8455	38420-38423	NN	denotes	ind
T8456	38402-38403	HYPH	denotes	-
T8457	38403-38406	NN	denotes	tTA
T8458	38407-38408	SYM	denotes	×
T8459	38409-38412	NN	denotes	tet
T8460	38412-38413	HYPH	denotes	-
T8461	38413-38419	NN	denotes	APPswe
T8462	38419-38420	HYPH	denotes	/
T8463	38429-38431	CD	denotes	18
T8464	38431-38432	:	denotes	;
T8465	38433-38434	NN	denotes	n
T8466	38437-38439	CD	denotes	22
T8467	38435-38436	SYM	denotes	=
T8468	38439-38440	-RRB-	denotes	)
T8469	38440-38441	.	denotes	.
T8470	38441-38634	sentence	denotes	Again, long-term dox treatment was begun at 6 mo of age, and mice were harvested after 3 mo of transgene suppression (6 mo untreated, n = 8; 9 mo untreated, n = 6; 6 mo + 3 mo treated, n = 8).
T8471	38442-38447	RB	denotes	Again
T8472	38477-38482	VBN	denotes	begun
T8473	38447-38449	,	denotes	,
T8474	38449-38453	JJ	denotes	long
T8475	38454-38458	NN	denotes	term
T8476	38453-38454	HYPH	denotes	-
T8477	38463-38472	NN	denotes	treatment
T8478	38459-38462	NN	denotes	dox
T8479	38473-38476	VBD	denotes	was
T8480	38483-38485	IN	denotes	at
T8481	38486-38487	CD	denotes	6
T8482	38488-38490	NN	denotes	mo
T8483	38491-38493	IN	denotes	of
T8484	38494-38497	NN	denotes	age
T8485	38497-38499	,	denotes	,
T8486	38499-38502	CC	denotes	and
T8487	38503-38507	NNS	denotes	mice
T8488	38513-38522	VBN	denotes	harvested
T8489	38508-38512	VBD	denotes	were
T8490	38523-38528	IN	denotes	after
T8491	38529-38530	CD	denotes	3
T8492	38531-38533	NN	denotes	mo
T8493	38534-38536	IN	denotes	of
T8494	38537-38546	NN	denotes	transgene
T8495	38547-38558	NN	denotes	suppression
T8496	38559-38560	-LRB-	denotes	(
T8497	38631-38632	CD	denotes	8
T8498	38560-38561	CD	denotes	6
T8499	38562-38564	NN	denotes	mo
T8500	38565-38574	JJ	denotes	untreated
T8501	38580-38581	CD	denotes	8
T8502	38574-38576	,	denotes	,
T8503	38576-38577	NN	denotes	n
T8504	38578-38579	SYM	denotes	=
T8505	38581-38582	:	denotes	;
T8506	38583-38584	CD	denotes	9
T8507	38585-38587	NN	denotes	mo
T8508	38588-38597	JJ	denotes	untreated
T8509	38603-38604	CD	denotes	6
T8510	38597-38599	,	denotes	,
T8511	38599-38600	NN	denotes	n
T8512	38601-38602	SYM	denotes	=
T8513	38604-38605	:	denotes	;
T8514	38606-38607	CD	denotes	6
T8515	38615-38617	NN	denotes	mo
T8516	38608-38610	NN	denotes	mo
T8517	38611-38612	SYM	denotes	+
T8518	38613-38614	CD	denotes	3
T8519	38618-38625	VBN	denotes	treated
T8520	38625-38627	,	denotes	,
T8521	38627-38628	NN	denotes	n
T8522	38629-38630	SYM	denotes	=
T8523	38632-38633	-RRB-	denotes	)
T8524	38633-38634	.	denotes	.
T8525	38634-38730	sentence	denotes	Immunoblotting for APP confirmed full transgene suppression in the treated animals (Figure S5).
T8526	38635-38649	NN	denotes	Immunoblotting
T8527	38658-38667	VBD	denotes	confirmed
T8528	38650-38653	IN	denotes	for
T8529	38654-38657	NN	denotes	APP
T8530	38668-38672	JJ	denotes	full
T8531	38683-38694	NN	denotes	suppression
T8532	38673-38682	NN	denotes	transgene
T8533	38695-38697	IN	denotes	in
T8534	38698-38701	DT	denotes	the
T8535	38710-38717	NNS	denotes	animals
T8536	38702-38709	VBN	denotes	treated
T8537	38718-38719	-LRB-	denotes	(
T8538	38726-38728	NN	denotes	S5
T8539	38719-38725	NN	denotes	Figure
T8540	38728-38729	-RRB-	denotes	)
T8541	38729-38730	.	denotes	.
T8542	38730-38858	sentence	denotes	As in the line 107 mice described above, amyloid burden worsened substantially in the untreated mice between 6 and 9 mo of age.
T8543	38731-38733	IN	denotes	As
T8544	38787-38795	VBD	denotes	worsened
T8545	38734-38736	IN	denotes	in
T8546	38737-38740	DT	denotes	the
T8547	38750-38754	NNS	denotes	mice
T8548	38741-38745	NN	denotes	line
T8549	38746-38749	CD	denotes	107
T8550	38755-38764	VBN	denotes	described
T8551	38765-38770	RB	denotes	above
T8552	38770-38772	,	denotes	,
T8553	38772-38779	NN	denotes	amyloid
T8554	38780-38786	NN	denotes	burden
T8555	38796-38809	RB	denotes	substantially
T8556	38810-38812	IN	denotes	in
T8557	38813-38816	DT	denotes	the
T8558	38827-38831	NNS	denotes	mice
T8559	38817-38826	JJ	denotes	untreated
T8560	38832-38839	IN	denotes	between
T8561	38840-38841	CD	denotes	6
T8562	38848-38850	NN	denotes	mo
T8563	38842-38845	CC	denotes	and
T8564	38846-38847	CD	denotes	9
T8565	38851-38853	IN	denotes	of
T8566	38854-38857	NN	denotes	age
T8567	38857-38858	.	denotes	.
T8568	38858-38991	sentence	denotes	Suppression of transgene expression abruptly arrested progression of pathology (Figure S6), but again without any sign of reduction.
T8569	38859-38870	NN	denotes	Suppression
T8570	38904-38912	VBD	denotes	arrested
T8571	38871-38873	IN	denotes	of
T8572	38874-38883	NN	denotes	transgene
T8573	38884-38894	NN	denotes	expression
T8574	38895-38903	RB	denotes	abruptly
T8575	38913-38924	NN	denotes	progression
T8576	38925-38927	IN	denotes	of
T8577	38928-38937	NN	denotes	pathology
T8578	38938-38939	-LRB-	denotes	(
T8579	38946-38948	NN	denotes	S6
T8580	38939-38945	NN	denotes	Figure
T8581	38948-38949	-RRB-	denotes	)
T8582	38949-38951	,	denotes	,
T8583	38951-38954	CC	denotes	but
T8584	38961-38968	IN	denotes	without
T8585	38955-38960	RB	denotes	again
T8586	38969-38972	DT	denotes	any
T8587	38973-38977	NN	denotes	sign
T8588	38978-38980	IN	denotes	of
T8589	38981-38990	NN	denotes	reduction
T8590	38990-38991	.	denotes	.
T8591	38991-39095	sentence	denotes	Both silver- and thioflavin-S-positive plaques could still be found in each of the dox-treated animals.
T8592	38992-38996	CC	denotes	Both
T8593	38997-39003	NN	denotes	silver
T8594	39022-39030	JJ	denotes	positive
T8595	39003-39004	HYPH	denotes	-
T8596	39005-39008	CC	denotes	and
T8597	39009-39019	NN	denotes	thioflavin
T8598	39020-39021	NN	denotes	S
T8599	39019-39020	HYPH	denotes	-
T8600	39021-39022	HYPH	denotes	-
T8601	39031-39038	NNS	denotes	plaques
T8602	39054-39059	VBN	denotes	found
T8603	39039-39044	MD	denotes	could
T8604	39045-39050	RB	denotes	still
T8605	39051-39053	VB	denotes	be
T8606	39060-39062	IN	denotes	in
T8607	39063-39067	DT	denotes	each
T8608	39068-39070	IN	denotes	of
T8609	39071-39074	DT	denotes	the
T8610	39087-39094	NNS	denotes	animals
T8611	39075-39078	NN	denotes	dox
T8612	39079-39086	VBN	denotes	treated
T8613	39078-39079	HYPH	denotes	-
T8614	39094-39095	.	denotes	.
T8615	39095-39278	sentence	denotes	We biochemically measured the amount of aggregated Aβ in the brains of our mice before and after transgene suppression using filter trap analysis of cortical tissue from each animal.
T8616	39096-39098	PRP	denotes	We
T8617	39113-39121	VBD	denotes	measured
T8618	39099-39112	RB	denotes	biochemically
T8619	39122-39125	DT	denotes	the
T8620	39126-39132	NN	denotes	amount
T8621	39133-39135	IN	denotes	of
T8622	39136-39146	VBN	denotes	aggregated
T8623	39147-39149	NN	denotes	Aβ
T8624	39150-39152	IN	denotes	in
T8625	39153-39156	DT	denotes	the
T8626	39157-39163	NNS	denotes	brains
T8627	39164-39166	IN	denotes	of
T8628	39167-39170	PRP$	denotes	our
T8629	39171-39175	NNS	denotes	mice
T8630	39176-39182	IN	denotes	before
T8631	39183-39186	CC	denotes	and
T8632	39187-39192	IN	denotes	after
T8633	39193-39202	NN	denotes	transgene
T8634	39203-39214	NN	denotes	suppression
T8635	39215-39220	VBG	denotes	using
T8636	39221-39227	NN	denotes	filter
T8637	39228-39232	NN	denotes	trap
T8638	39233-39241	NN	denotes	analysis
T8639	39242-39244	IN	denotes	of
T8640	39245-39253	JJ	denotes	cortical
T8641	39254-39260	NN	denotes	tissue
T8642	39261-39265	IN	denotes	from
T8643	39266-39270	DT	denotes	each
T8644	39271-39277	NN	denotes	animal
T8645	39277-39278	.	denotes	.
T8646	39278-39501	sentence	denotes	In this assay, serial dilutions of protein homogenate are passed through a cellulose acetate filter; particles larger than the pore size of the filter become trapped in the membrane and are revealed by immunoblotting [22].
T8647	39279-39281	IN	denotes	In
T8648	39337-39343	VBN	denotes	passed
T8649	39282-39286	DT	denotes	this
T8650	39287-39292	NN	denotes	assay
T8651	39292-39294	,	denotes	,
T8652	39294-39300	JJ	denotes	serial
T8653	39301-39310	NNS	denotes	dilutions
T8654	39311-39313	IN	denotes	of
T8655	39314-39321	NN	denotes	protein
T8656	39322-39332	NN	denotes	homogenate
T8657	39333-39336	VBP	denotes	are
T8658	39430-39436	VBP	denotes	become
T8659	39344-39351	IN	denotes	through
T8660	39352-39353	DT	denotes	a
T8661	39372-39378	NN	denotes	filter
T8662	39354-39363	NN	denotes	cellulose
T8663	39364-39371	NN	denotes	acetate
T8664	39378-39379	:	denotes	;
T8665	39380-39389	NNS	denotes	particles
T8666	39390-39396	JJR	denotes	larger
T8667	39397-39401	IN	denotes	than
T8668	39402-39405	DT	denotes	the
T8669	39411-39415	NN	denotes	size
T8670	39406-39410	NN	denotes	pore
T8671	39416-39418	IN	denotes	of
T8672	39419-39422	DT	denotes	the
T8673	39423-39429	NN	denotes	filter
T8674	39437-39444	JJ	denotes	trapped
T8675	39445-39447	IN	denotes	in
T8676	39448-39451	DT	denotes	the
T8677	39452-39460	NN	denotes	membrane
T8678	39461-39464	CC	denotes	and
T8679	39465-39468	VBP	denotes	are
T8680	39469-39477	VBN	denotes	revealed
T8681	39478-39480	IN	denotes	by
T8682	39481-39495	NN	denotes	immunoblotting
T8683	39496-39497	-LRB-	denotes	[
T8684	39497-39499	CD	denotes	22
T8685	39499-39500	-RRB-	denotes	]
T8686	39500-39501	.	denotes	.
T8687	39501-39723	sentence	denotes	Consistent with our visual analysis of the histological sections, line 107 tTA/APP mice treated with dox for 3 or 6 mo had the same amount of aggregated Aβ as when they started treatment at 6 mo of age (Figure 4A and 4B).
T8688	39502-39512	JJ	denotes	Consistent
T8689	39621-39624	VBD	denotes	had
T8690	39513-39517	IN	denotes	with
T8691	39518-39521	PRP$	denotes	our
T8692	39529-39537	NN	denotes	analysis
T8693	39522-39528	JJ	denotes	visual
T8694	39538-39540	IN	denotes	of
T8695	39541-39544	DT	denotes	the
T8696	39558-39566	NNS	denotes	sections
T8697	39545-39557	JJ	denotes	histological
T8698	39566-39568	,	denotes	,
T8699	39568-39572	NN	denotes	line
T8700	39585-39589	NNS	denotes	mice
T8701	39573-39576	CD	denotes	107
T8702	39577-39580	NN	denotes	tTA
T8703	39581-39584	NN	denotes	APP
T8704	39580-39581	HYPH	denotes	/
T8705	39590-39597	VBN	denotes	treated
T8706	39598-39602	IN	denotes	with
T8707	39603-39606	NN	denotes	dox
T8708	39607-39610	IN	denotes	for
T8709	39611-39612	CD	denotes	3
T8710	39618-39620	NN	denotes	mo
T8711	39613-39615	CC	denotes	or
T8712	39616-39617	CD	denotes	6
T8713	39625-39628	DT	denotes	the
T8714	39634-39640	NN	denotes	amount
T8715	39629-39633	JJ	denotes	same
T8716	39641-39643	IN	denotes	of
T8717	39644-39654	VBN	denotes	aggregated
T8718	39655-39657	NN	denotes	Aβ
T8719	39658-39660	IN	denotes	as
T8720	39661-39665	WRB	denotes	when
T8721	39671-39678	VBD	denotes	started
T8722	39666-39670	PRP	denotes	they
T8723	39679-39688	NN	denotes	treatment
T8724	39689-39691	IN	denotes	at
T8725	39692-39693	CD	denotes	6
T8726	39694-39696	NN	denotes	mo
T8727	39697-39699	IN	denotes	of
T8728	39700-39703	NN	denotes	age
T8729	39704-39705	-LRB-	denotes	(
T8730	39712-39714	NN	denotes	4A
T8731	39705-39711	NN	denotes	Figure
T8732	39715-39718	CC	denotes	and
T8733	39719-39721	NN	denotes	4B
T8734	39721-39722	-RRB-	denotes	)
T8735	39722-39723	.	denotes	.
T8736	39723-39832	sentence	denotes	In contrast, untreated 9-mo-old mice had almost twice as much aggregated Aβ as either of the treated groups.
T8737	39724-39726	IN	denotes	In
T8738	39761-39764	VBD	denotes	had
T8739	39727-39735	NN	denotes	contrast
T8740	39735-39737	,	denotes	,
T8741	39737-39746	JJ	denotes	untreated
T8742	39756-39760	NNS	denotes	mice
T8743	39747-39748	CD	denotes	9
T8744	39749-39751	NN	denotes	mo
T8745	39748-39749	HYPH	denotes	-
T8746	39752-39755	JJ	denotes	old
T8747	39751-39752	HYPH	denotes	-
T8748	39765-39771	RB	denotes	almost
T8749	39772-39777	RB	denotes	twice
T8750	39781-39785	JJ	denotes	much
T8751	39778-39780	RB	denotes	as
T8752	39797-39799	NN	denotes	Aβ
T8753	39786-39796	VBN	denotes	aggregated
T8754	39800-39802	IN	denotes	as
T8755	39803-39809	DT	denotes	either
T8756	39810-39812	IN	denotes	of
T8757	39813-39816	DT	denotes	the
T8758	39825-39831	NNS	denotes	groups
T8759	39817-39824	VBN	denotes	treated
T8760	39831-39832	.	denotes	.
T8761	39832-40059	sentence	denotes	Filter trap analysis of line 18 tTA/APP mice yielded similar results: the increase in aggregated Aβ observed in untreated animals between 6 and 9 mo of age was completely arrested by transgene suppression (Figure S5C and S5D).
T8762	39833-39839	NN	denotes	Filter
T8763	39845-39853	NN	denotes	analysis
T8764	39840-39844	NN	denotes	trap
T8765	39878-39885	VBD	denotes	yielded
T8766	39854-39856	IN	denotes	of
T8767	39857-39861	NN	denotes	line
T8768	39873-39877	NNS	denotes	mice
T8769	39862-39864	CD	denotes	18
T8770	39865-39868	NN	denotes	tTA
T8771	39869-39872	NN	denotes	APP
T8772	39868-39869	HYPH	denotes	/
T8773	40004-40012	VBN	denotes	arrested
T8774	39886-39893	JJ	denotes	similar
T8775	39894-39901	NNS	denotes	results
T8776	39901-39903	:	denotes	:
T8777	39903-39906	DT	denotes	the
T8778	39907-39915	NN	denotes	increase
T8779	39916-39918	IN	denotes	in
T8780	39919-39929	VBN	denotes	aggregated
T8781	39930-39932	NN	denotes	Aβ
T8782	39933-39941	VBN	denotes	observed
T8783	39942-39944	IN	denotes	in
T8784	39945-39954	JJ	denotes	untreated
T8785	39955-39962	NNS	denotes	animals
T8786	39963-39970	IN	denotes	between
T8787	39971-39972	CD	denotes	6
T8788	39979-39981	NN	denotes	mo
T8789	39973-39976	CC	denotes	and
T8790	39977-39978	CD	denotes	9
T8791	39982-39984	IN	denotes	of
T8792	39985-39988	NN	denotes	age
T8793	39989-39992	VBD	denotes	was
T8794	39993-40003	RB	denotes	completely
T8795	40013-40015	IN	denotes	by
T8796	40016-40025	NN	denotes	transgene
T8797	40026-40037	NN	denotes	suppression
T8798	40038-40039	-LRB-	denotes	(
T8799	40046-40049	NN	denotes	S5C
T8800	40039-40045	NN	denotes	Figure
T8801	40050-40053	CC	denotes	and
T8802	40054-40057	NN	denotes	S5D
T8803	40057-40058	-RRB-	denotes	)
T8804	40058-40059	.	denotes	.
T8805	40059-40250	sentence	denotes	We next used ELISA to measure total Aβ in the brains of each group to determine whether any change in the amount or solubility of peptide occurred while APPswe/ind expression was suppressed.
T8806	40060-40062	PRP	denotes	We
T8807	40068-40072	VBD	denotes	used
T8808	40063-40067	RB	denotes	next
T8809	40073-40078	NN	denotes	ELISA
T8810	40079-40081	TO	denotes	to
T8811	40082-40089	VB	denotes	measure
T8812	40090-40095	JJ	denotes	total
T8813	40096-40098	NN	denotes	Aβ
T8814	40099-40101	IN	denotes	in
T8815	40102-40105	DT	denotes	the
T8816	40106-40112	NNS	denotes	brains
T8817	40113-40115	IN	denotes	of
T8818	40116-40120	DT	denotes	each
T8819	40121-40126	NN	denotes	group
T8820	40127-40129	TO	denotes	to
T8821	40130-40139	VB	denotes	determine
T8822	40140-40147	IN	denotes	whether
T8823	40198-40206	VBD	denotes	occurred
T8824	40148-40151	DT	denotes	any
T8825	40152-40158	NN	denotes	change
T8826	40159-40161	IN	denotes	in
T8827	40162-40165	DT	denotes	the
T8828	40166-40172	NN	denotes	amount
T8829	40173-40175	CC	denotes	or
T8830	40176-40186	NN	denotes	solubility
T8831	40187-40189	IN	denotes	of
T8832	40190-40197	NN	denotes	peptide
T8833	40207-40212	IN	denotes	while
T8834	40239-40249	VBN	denotes	suppressed
T8835	40213-40219	NN	denotes	APPswe
T8836	40220-40223	NN	denotes	ind
T8837	40219-40220	HYPH	denotes	/
T8838	40224-40234	NN	denotes	expression
T8839	40235-40238	VBD	denotes	was
T8840	40249-40250	.	denotes	.
T8841	40250-40443	sentence	denotes	Cortical homogenates were sequentially extracted to separate peptide into PBS-, SDS-, and FA-soluble fractions, then transgene-derived Aβ40 and Aβ42 were measured by human-specific ELISA [23].
T8842	40251-40259	JJ	denotes	Cortical
T8843	40260-40271	NNS	denotes	homogenates
T8844	40290-40299	VBN	denotes	extracted
T8845	40272-40276	VBD	denotes	were
T8846	40277-40289	RB	denotes	sequentially
T8847	40405-40413	VBN	denotes	measured
T8848	40300-40302	TO	denotes	to
T8849	40303-40311	VB	denotes	separate
T8850	40312-40319	NN	denotes	peptide
T8851	40320-40324	IN	denotes	into
T8852	40325-40328	NN	denotes	PBS
T8853	40344-40351	JJ	denotes	soluble
T8854	40328-40329	HYPH	denotes	-
T8855	40329-40331	,	denotes	,
T8856	40331-40334	NN	denotes	SDS
T8857	40334-40335	HYPH	denotes	-
T8858	40335-40337	,	denotes	,
T8859	40337-40340	CC	denotes	and
T8860	40341-40343	NN	denotes	FA
T8861	40343-40344	HYPH	denotes	-
T8862	40352-40361	NNS	denotes	fractions
T8863	40361-40363	,	denotes	,
T8864	40363-40367	RB	denotes	then
T8865	40368-40377	NN	denotes	transgene
T8866	40378-40385	VBN	denotes	derived
T8867	40377-40378	HYPH	denotes	-
T8868	40386-40390	NN	denotes	Aβ40
T8869	40391-40394	CC	denotes	and
T8870	40395-40399	NN	denotes	Aβ42
T8871	40400-40404	VBD	denotes	were
T8872	40414-40416	IN	denotes	by
T8873	40417-40422	JJ	denotes	human
T8874	40423-40431	JJ	denotes	specific
T8875	40422-40423	HYPH	denotes	-
T8876	40432-40437	NN	denotes	ELISA
T8877	40438-40439	-LRB-	denotes	[
T8878	40439-40441	CD	denotes	23
T8879	40441-40442	-RRB-	denotes	]
T8880	40442-40443	.	denotes	.
T8881	40443-40595	sentence	denotes	In all animals harboring amyloid deposits, we found that the vast majority of Aβ (>99%) was extracted into the SDS and FA fractions (Figure 5A and 5B).
T8882	40444-40446	IN	denotes	In
T8883	40490-40495	VBD	denotes	found
T8884	40447-40450	DT	denotes	all
T8885	40451-40458	NNS	denotes	animals
T8886	40459-40468	VBG	denotes	harboring
T8887	40469-40476	NN	denotes	amyloid
T8888	40477-40485	NNS	denotes	deposits
T8889	40485-40487	,	denotes	,
T8890	40487-40489	PRP	denotes	we
T8891	40496-40500	IN	denotes	that
T8892	40536-40545	VBN	denotes	extracted
T8893	40501-40504	DT	denotes	the
T8894	40510-40518	NN	denotes	majority
T8895	40505-40509	JJ	denotes	vast
T8896	40519-40521	IN	denotes	of
T8897	40522-40524	NN	denotes	Aβ
T8898	40525-40526	-LRB-	denotes	(
T8899	40529-40530	NN	denotes	%
T8900	40526-40527	SYM	denotes	>
T8901	40527-40529	CD	denotes	99
T8902	40530-40531	-RRB-	denotes	)
T8903	40532-40535	VBD	denotes	was
T8904	40546-40550	IN	denotes	into
T8905	40551-40554	DT	denotes	the
T8906	40566-40575	NNS	denotes	fractions
T8907	40555-40558	NN	denotes	SDS
T8908	40559-40562	CC	denotes	and
T8909	40563-40565	NN	denotes	FA
T8910	40576-40577	-LRB-	denotes	(
T8911	40584-40586	NN	denotes	5A
T8912	40577-40583	NN	denotes	Figure
T8913	40587-40590	CC	denotes	and
T8914	40591-40593	NN	denotes	5B
T8915	40593-40594	-RRB-	denotes	)
T8916	40594-40595	.	denotes	.
T8917	40595-40816	sentence	denotes	Consistent with the filter trap results presented above, there were no significant differences in SDS- or FA-soluble Aβ between the 6 mo untreated cohort and either the 6 mo + 3 mo treated or 6 mo + 6 mo treated cohorts.
T8918	40596-40606	JJ	denotes	Consistent
T8919	40659-40663	VBD	denotes	were
T8920	40607-40611	IN	denotes	with
T8921	40612-40615	DT	denotes	the
T8922	40628-40635	NNS	denotes	results
T8923	40616-40622	NN	denotes	filter
T8924	40623-40627	NN	denotes	trap
T8925	40636-40645	VBN	denotes	presented
T8926	40646-40651	RB	denotes	above
T8927	40651-40653	,	denotes	,
T8928	40653-40658	EX	denotes	there
T8929	40664-40666	DT	denotes	no
T8930	40679-40690	NNS	denotes	differences
T8931	40667-40678	JJ	denotes	significant
T8932	40691-40693	IN	denotes	in
T8933	40694-40697	NN	denotes	SDS
T8934	40705-40712	JJ	denotes	soluble
T8935	40697-40698	HYPH	denotes	-
T8936	40699-40701	CC	denotes	or
T8937	40702-40704	NN	denotes	FA
T8938	40704-40705	HYPH	denotes	-
T8939	40713-40715	NN	denotes	Aβ
T8940	40716-40723	IN	denotes	between
T8941	40724-40727	DT	denotes	the
T8942	40743-40749	NN	denotes	cohort
T8943	40728-40729	CD	denotes	6
T8944	40730-40732	NN	denotes	mo
T8945	40733-40742	JJ	denotes	untreated
T8946	40750-40753	CC	denotes	and
T8947	40754-40760	CC	denotes	either
T8948	40808-40815	NNS	denotes	cohorts
T8949	40761-40764	DT	denotes	the
T8950	40765-40766	CD	denotes	6
T8951	40767-40769	NN	denotes	mo
T8952	40770-40771	SYM	denotes	+
T8953	40772-40773	CD	denotes	3
T8954	40774-40776	NN	denotes	mo
T8955	40777-40784	VBN	denotes	treated
T8956	40785-40787	CC	denotes	or
T8957	40788-40789	CD	denotes	6
T8958	40790-40792	NN	denotes	mo
T8959	40793-40794	SYM	denotes	+
T8960	40795-40796	CD	denotes	6
T8961	40797-40799	NN	denotes	mo
T8962	40800-40807	VBN	denotes	treated
T8963	40815-40816	.	denotes	.
T8964	40816-40973	sentence	denotes	However, brains of both 6 mo + 3 mo and 6 mo + 6 mo treated cohorts contained roughly twice as much PBS-soluble Aβ40 as untreated 6-mo-old mice (Figure 5C).
T8965	40817-40824	RB	denotes	However
T8966	40885-40894	VBD	denotes	contained
T8967	40824-40826	,	denotes	,
T8968	40826-40832	NNS	denotes	brains
T8969	40833-40835	IN	denotes	of
T8970	40836-40840	CC	denotes	both
T8971	40843-40845	NN	denotes	mo
T8972	40841-40842	CD	denotes	6
T8973	40869-40876	VBN	denotes	treated
T8974	40846-40847	SYM	denotes	+
T8975	40848-40849	CD	denotes	3
T8976	40850-40852	NN	denotes	mo
T8977	40853-40856	CC	denotes	and
T8978	40857-40858	CD	denotes	6
T8979	40859-40861	NN	denotes	mo
T8980	40862-40863	SYM	denotes	+
T8981	40864-40865	CD	denotes	6
T8982	40866-40868	NN	denotes	mo
T8983	40877-40884	NNS	denotes	cohorts
T8984	40895-40902	RB	denotes	roughly
T8985	40903-40908	RB	denotes	twice
T8986	40912-40916	JJ	denotes	much
T8987	40909-40911	RB	denotes	as
T8988	40929-40933	NN	denotes	Aβ40
T8989	40917-40920	NN	denotes	PBS
T8990	40920-40921	HYPH	denotes	-
T8991	40921-40928	JJ	denotes	soluble
T8992	40934-40936	IN	denotes	as
T8993	40937-40946	JJ	denotes	untreated
T8994	40956-40960	NNS	denotes	mice
T8995	40947-40948	CD	denotes	6
T8996	40949-40951	NN	denotes	mo
T8997	40948-40949	HYPH	denotes	-
T8998	40952-40955	JJ	denotes	old
T8999	40951-40952	HYPH	denotes	-
T9000	40961-40962	-LRB-	denotes	(
T9001	40969-40971	NN	denotes	5C
T9002	40962-40968	NN	denotes	Figure
T9003	40971-40972	-RRB-	denotes	)
T9004	40972-40973	.	denotes	.
T9005	40973-41056	sentence	denotes	Levels of Aβ42 showed a similar trend, but did not reach statistical significance.
T9006	40974-40980	NNS	denotes	Levels
T9007	40989-40995	VBD	denotes	showed
T9008	40981-40983	IN	denotes	of
T9009	40984-40988	NN	denotes	Aβ42
T9010	40996-40997	DT	denotes	a
T9011	41006-41011	NN	denotes	trend
T9012	40998-41005	JJ	denotes	similar
T9013	41011-41013	,	denotes	,
T9014	41013-41016	CC	denotes	but
T9015	41017-41020	VBD	denotes	did
T9016	41025-41030	VB	denotes	reach
T9017	41021-41024	RB	denotes	not
T9018	41031-41042	JJ	denotes	statistical
T9019	41043-41055	NN	denotes	significance
T9020	41055-41056	.	denotes	.
T9021	41056-41373	sentence	denotes	In fact, levels of PBS-soluble Aβ40 and Aβ42 in the 6 mo + 3 mo and 6 mo + 6 mo treated cohorts were most similar to that of the 9 mo untreated cohort, suggesting that age, as opposed to synthetic rate (which would be negligible in the treated animals), may determine the fraction of PBS-soluble Aβ in these animals.
T9022	41057-41059	IN	denotes	In
T9023	41153-41157	VBD	denotes	were
T9024	41060-41064	NN	denotes	fact
T9025	41064-41066	,	denotes	,
T9026	41066-41072	NNS	denotes	levels
T9027	41073-41075	IN	denotes	of
T9028	41076-41079	NN	denotes	PBS
T9029	41080-41087	JJ	denotes	soluble
T9030	41079-41080	HYPH	denotes	-
T9031	41088-41092	NN	denotes	Aβ40
T9032	41093-41096	CC	denotes	and
T9033	41097-41101	NN	denotes	Aβ42
T9034	41102-41104	IN	denotes	in
T9035	41105-41108	DT	denotes	the
T9036	41145-41152	NNS	denotes	cohorts
T9037	41109-41110	CD	denotes	6
T9038	41111-41113	NN	denotes	mo
T9039	41114-41115	SYM	denotes	+
T9040	41116-41117	CD	denotes	3
T9041	41118-41120	NN	denotes	mo
T9042	41121-41124	CC	denotes	and
T9043	41125-41126	CD	denotes	6
T9044	41127-41129	NN	denotes	mo
T9045	41130-41131	SYM	denotes	+
T9046	41132-41133	CD	denotes	6
T9047	41134-41136	NN	denotes	mo
T9048	41137-41144	VBN	denotes	treated
T9049	41158-41162	RBS	denotes	most
T9050	41163-41170	JJ	denotes	similar
T9051	41171-41173	IN	denotes	to
T9052	41174-41178	DT	denotes	that
T9053	41179-41181	IN	denotes	of
T9054	41182-41185	DT	denotes	the
T9055	41201-41207	NN	denotes	cohort
T9056	41186-41187	CD	denotes	9
T9057	41188-41190	NN	denotes	mo
T9058	41191-41200	JJ	denotes	untreated
T9059	41207-41209	,	denotes	,
T9060	41209-41219	VBG	denotes	suggesting
T9061	41220-41224	IN	denotes	that
T9062	41315-41324	VB	denotes	determine
T9063	41225-41228	NN	denotes	age
T9064	41228-41230	,	denotes	,
T9065	41230-41232	IN	denotes	as
T9066	41233-41240	VBN	denotes	opposed
T9067	41241-41243	IN	denotes	to
T9068	41244-41253	JJ	denotes	synthetic
T9069	41254-41258	NN	denotes	rate
T9070	41259-41260	-LRB-	denotes	(
T9071	41260-41265	WDT	denotes	which
T9072	41272-41274	VB	denotes	be
T9073	41266-41271	MD	denotes	would
T9074	41275-41285	JJ	denotes	negligible
T9075	41286-41288	IN	denotes	in
T9076	41289-41292	DT	denotes	the
T9077	41301-41308	NNS	denotes	animals
T9078	41293-41300	VBN	denotes	treated
T9079	41308-41309	-RRB-	denotes	)
T9080	41309-41311	,	denotes	,
T9081	41311-41314	MD	denotes	may
T9082	41325-41328	DT	denotes	the
T9083	41329-41337	NN	denotes	fraction
T9084	41338-41340	IN	denotes	of
T9085	41341-41344	NN	denotes	PBS
T9086	41345-41352	JJ	denotes	soluble
T9087	41344-41345	HYPH	denotes	-
T9088	41353-41355	NN	denotes	Aβ
T9089	41356-41358	IN	denotes	in
T9090	41359-41364	DT	denotes	these
T9091	41365-41372	NNS	denotes	animals
T9092	41372-41373	.	denotes	.
T9093	41373-43701	sentence	denotes	Figure 5 Aβ ELISA Confirms Arrest of Progression without Clearance of Peptide in Mice with Preexisting Aggregates Aβ levels in untreated 6- and 9-mo-old tTA/APP line 107 mice (shown in Figure 4) were compared to those in 9- and 12-mo-old animals treated with dox from the age of 6 mo. Single transgenic APP samples were included as negative controls. Cortical homogenates were fractionated by sequential multi-step extraction with PBS, 2% SDS, and 70% FA followed by human-specific Aβ ELISA to measure transgene-derived peptide in each fraction. Aβ40 is shown in white, Aβ42 in black. (A and B) Most Aβ in the brains of plaque-bearing mice is extracted into the FA and SDS fractions. Consistent with amyloid burden (Figures 4 and Figure S3), SDS- and FA-extracted Aβ levels in untreated 9-mo-old mice were significantly higher than in untreated 6-mo-old mice (Tukey post-hoc test applied to significant effect of group ANOVA for SDS and FA fractions F 3,18 = 4.72–12.92, p < 0.02). In contrast, 3 or 6 mo of transgene suppression held Aβ at levels equivalent to those harbored when treatment was started (p > 0.2 compared to 6 mo untreated mice, Tukey post-hoc test). , p < 0.05; , p < 0.005; **, p < 0.001 versus 9-mo-old untreated mice, Tukey post-hoc test. Significance for APP versus 9-mo-old untreated mice is based on Student's t-test. (C) The PBS fraction represents less than 0.1% of total Aβ (note the change in y-axis from [A] and [B]), but only here do Aβ levels in the dox-treated mice differ from those in younger untreated mice. Although both peptides appear elevated in the treated groups compared to the untreated 6-mo-old mice, only Aβ40 reaches statistical significance (p < 0.05, Tukey post-hoc test applied to significant effect of group ANOVA for Aβ40 F 3,18 = 4.60, p < 0.02). A similar trend was seen for Aβ42, where ANOVA yielded a significant effect of group for PBS-soluble Aβ42 (F 3,18 = 3.75, p < 0.03), however this was due only to differences between the untreated 6- and 9-mo-old groups. •, p < 0.05 versus 6-mo-old untreated mice, Tukey post-hoc test; •••, p < 0.001 versus 6-mo-old untreated mice, Student's t-test. We also assessed neuritic and glial pathology surrounding the plaques to determine whether there were any changes in nearby tissue following long-term transgene suppression.
T9094	43528-43530	PRP	denotes	We
T9095	43536-43544	VBD	denotes	assessed
T9096	43531-43535	RB	denotes	also
T9097	43545-43553	JJ	denotes	neuritic
T9098	43564-43573	NN	denotes	pathology
T9099	43554-43557	CC	denotes	and
T9100	43558-43563	JJ	denotes	glial
T9101	43574-43585	VBG	denotes	surrounding
T9102	43586-43589	DT	denotes	the
T9103	43590-43597	NNS	denotes	plaques
T9104	43598-43600	TO	denotes	to
T9105	43601-43610	VB	denotes	determine
T9106	43611-43618	IN	denotes	whether
T9107	43625-43629	VBD	denotes	were
T9108	43619-43624	EX	denotes	there
T9109	43630-43633	DT	denotes	any
T9110	43634-43641	NNS	denotes	changes
T9111	43642-43644	IN	denotes	in
T9112	43645-43651	JJ	denotes	nearby
T9113	43652-43658	NN	denotes	tissue
T9114	43659-43668	VBG	denotes	following
T9115	43669-43673	JJ	denotes	long
T9116	43674-43678	NN	denotes	term
T9117	43673-43674	HYPH	denotes	-
T9118	43689-43700	NN	denotes	suppression
T9119	43679-43688	NN	denotes	transgene
T9120	43700-43701	.	denotes	.
T9121	43701-43817	sentence	denotes	Both Hirano silver stain and ubiquitin immunostaining showed neuritic pathology in all treatment groups (Figure 6).
T9122	43702-43706	CC	denotes	Both
T9123	43721-43726	NN	denotes	stain
T9124	43707-43713	NNP	denotes	Hirano
T9125	43714-43720	NN	denotes	silver
T9126	43756-43762	VBD	denotes	showed
T9127	43727-43730	CC	denotes	and
T9128	43731-43740	NN	denotes	ubiquitin
T9129	43741-43755	NN	denotes	immunostaining
T9130	43763-43771	JJ	denotes	neuritic
T9131	43772-43781	NN	denotes	pathology
T9132	43782-43784	IN	denotes	in
T9133	43785-43788	DT	denotes	all
T9134	43799-43805	NNS	denotes	groups
T9135	43789-43798	NN	denotes	treatment
T9136	43806-43807	-LRB-	denotes	(
T9137	43807-43813	NN	denotes	Figure
T9138	43814-43815	CD	denotes	6
T9139	43815-43816	-RRB-	denotes	)
T9140	43816-43817	.	denotes	.
T9141	43817-43923	sentence	denotes	Similarly, activated astrocytes immunostained for GFAP were found near plaques in all animals (Figure 6).
T9142	43818-43827	RB	denotes	Similarly
T9143	43878-43883	VBN	denotes	found
T9144	43827-43829	,	denotes	,
T9145	43829-43838	VBN	denotes	activated
T9146	43839-43849	NNS	denotes	astrocytes
T9147	43850-43863	VBN	denotes	immunostained
T9148	43864-43867	IN	denotes	for
T9149	43868-43872	NN	denotes	GFAP
T9150	43873-43877	VBD	denotes	were
T9151	43884-43888	IN	denotes	near
T9152	43889-43896	NNS	denotes	plaques
T9153	43897-43899	IN	denotes	in
T9154	43900-43903	DT	denotes	all
T9155	43904-43911	NNS	denotes	animals
T9156	43912-43913	-LRB-	denotes	(
T9157	43913-43919	NN	denotes	Figure
T9158	43920-43921	CD	denotes	6
T9159	43921-43922	-RRB-	denotes	)
T9160	43922-43923	.	denotes	.
T9161	43923-43998	sentence	denotes	Neuritic and glial pathology were more severe in the older untreated mice.
T9162	43924-43932	JJ	denotes	Neuritic
T9163	43943-43952	NN	denotes	pathology
T9164	43933-43936	CC	denotes	and
T9165	43937-43942	JJ	denotes	glial
T9166	43953-43957	VBD	denotes	were
T9167	43958-43962	RBR	denotes	more
T9168	43963-43969	JJ	denotes	severe
T9169	43970-43972	IN	denotes	in
T9170	43973-43976	DT	denotes	the
T9171	43993-43997	NNS	denotes	mice
T9172	43977-43982	JJR	denotes	older
T9173	43983-43992	JJ	denotes	untreated
T9174	43997-43998	.	denotes	.
T9175	43998-44193	sentence	denotes	In contrast, transgene suppression prevented the growth of individual deposits apparent in untreated mice, and limited the surrounding pathology to what was already present when treatment began.
T9176	43999-44001	IN	denotes	In
T9177	44034-44043	VBD	denotes	prevented
T9178	44002-44010	NN	denotes	contrast
T9179	44010-44012	,	denotes	,
T9180	44012-44021	NN	denotes	transgene
T9181	44022-44033	NN	denotes	suppression
T9182	44044-44047	DT	denotes	the
T9183	44048-44054	NN	denotes	growth
T9184	44055-44057	IN	denotes	of
T9185	44058-44068	JJ	denotes	individual
T9186	44069-44077	NNS	denotes	deposits
T9187	44078-44086	JJ	denotes	apparent
T9188	44087-44089	IN	denotes	in
T9189	44090-44099	JJ	denotes	untreated
T9190	44100-44104	NNS	denotes	mice
T9191	44104-44106	,	denotes	,
T9192	44106-44109	CC	denotes	and
T9193	44110-44117	VBD	denotes	limited
T9194	44118-44121	DT	denotes	the
T9195	44134-44143	NN	denotes	pathology
T9196	44122-44133	VBG	denotes	surrounding
T9197	44144-44146	IN	denotes	to
T9198	44147-44151	WP	denotes	what
T9199	44152-44155	VBD	denotes	was
T9200	44156-44163	RB	denotes	already
T9201	44164-44171	JJ	denotes	present
T9202	44172-44176	WRB	denotes	when
T9203	44187-44192	VBD	denotes	began
T9204	44177-44186	NN	denotes	treatment
T9205	44192-44193	.	denotes	.
T9206	44193-44956	sentence	denotes	Figure 6 Neuritic and Glial Pathology Are Unchanged following Transgene Suppression Dystrophic neurites and activated astrocytes surround most compact plaques in tet-off APP mice (line 107). Dark-stained, ubiquitin-filled neurites and reactive astrocytes form a halo around cored, fibrillar deposits by 6 mo of age that worsens with time in untreated mice. Both plaque-associated pathologies are arrested, although not reversed, by transgene suppression. Hirano silver stain (top row); GFAP immunohistochemistry (middle row); ubiquitin immunohistochemistry (bottom row). An obvious question we sought to address was whether the deposition of Aβ diminished cognitive ability in untreated mice, and what might happen to cognition when the process was interrupted.
T9207	44766-44768	DT	denotes	An
T9208	44777-44785	NN	denotes	question
T9209	44769-44776	JJ	denotes	obvious
T9210	44807-44810	VBD	denotes	was
T9211	44786-44788	PRP	denotes	we
T9212	44789-44795	VBD	denotes	sought
T9213	44796-44798	TO	denotes	to
T9214	44799-44806	VB	denotes	address
T9215	44811-44818	IN	denotes	whether
T9216	44840-44850	VBD	denotes	diminished
T9217	44819-44822	DT	denotes	the
T9218	44823-44833	NN	denotes	deposition
T9219	44834-44836	IN	denotes	of
T9220	44837-44839	NN	denotes	Aβ
T9221	44851-44860	JJ	denotes	cognitive
T9222	44861-44868	NN	denotes	ability
T9223	44869-44871	IN	denotes	in
T9224	44872-44881	JJ	denotes	untreated
T9225	44882-44886	NNS	denotes	mice
T9226	44886-44888	,	denotes	,
T9227	44888-44891	CC	denotes	and
T9228	44892-44896	WP	denotes	what
T9229	44903-44909	VB	denotes	happen
T9230	44897-44902	MD	denotes	might
T9231	44910-44912	IN	denotes	to
T9232	44913-44922	NN	denotes	cognition
T9233	44923-44927	WRB	denotes	when
T9234	44944-44955	VBN	denotes	interrupted
T9235	44928-44931	DT	denotes	the
T9236	44932-44939	NN	denotes	process
T9237	44940-44943	VBD	denotes	was
T9238	44955-44956	.	denotes	.
T9239	44956-45092	sentence	denotes	Unfortunately, efforts to characterize cognitive behavior were compromised by severe hyperactivity in untreated double transgenic mice.
T9240	44957-44970	RB	denotes	Unfortunately
T9241	45020-45031	VBN	denotes	compromised
T9242	44970-44972	,	denotes	,
T9243	44972-44979	NNS	denotes	efforts
T9244	44980-44982	TO	denotes	to
T9245	44983-44995	VB	denotes	characterize
T9246	44996-45005	JJ	denotes	cognitive
T9247	45006-45014	NN	denotes	behavior
T9248	45015-45019	VBD	denotes	were
T9249	45032-45034	IN	denotes	by
T9250	45035-45041	JJ	denotes	severe
T9251	45042-45055	NN	denotes	hyperactivity
T9252	45056-45058	IN	denotes	in
T9253	45059-45068	JJ	denotes	untreated
T9254	45087-45091	NNS	denotes	mice
T9255	45069-45075	JJ	denotes	double
T9256	45076-45086	JJ	denotes	transgenic
T9257	45091-45092	.	denotes	.
T9258	45092-45277	sentence	denotes	The tTA/APP animals were often seen running in circles around the perimeter of their cages, and a similar swimming pattern was noted when the mice were tested in the Morris water maze.
T9259	45093-45096	DT	denotes	The
T9260	45105-45112	NNS	denotes	animals
T9261	45097-45100	NN	denotes	tTA
T9262	45101-45104	NN	denotes	APP
T9263	45100-45101	HYPH	denotes	/
T9264	45124-45128	VBN	denotes	seen
T9265	45113-45117	VBD	denotes	were
T9266	45118-45123	RB	denotes	often
T9267	45129-45136	VBG	denotes	running
T9268	45137-45139	IN	denotes	in
T9269	45140-45147	NNS	denotes	circles
T9270	45148-45154	IN	denotes	around
T9271	45155-45158	DT	denotes	the
T9272	45159-45168	NN	denotes	perimeter
T9273	45169-45171	IN	denotes	of
T9274	45172-45177	PRP$	denotes	their
T9275	45178-45183	NNS	denotes	cages
T9276	45183-45185	,	denotes	,
T9277	45185-45188	CC	denotes	and
T9278	45189-45190	DT	denotes	a
T9279	45208-45215	NN	denotes	pattern
T9280	45191-45198	JJ	denotes	similar
T9281	45199-45207	NN	denotes	swimming
T9282	45220-45225	VBN	denotes	noted
T9283	45216-45219	VBD	denotes	was
T9284	45226-45230	WRB	denotes	when
T9285	45245-45251	VBN	denotes	tested
T9286	45231-45234	DT	denotes	the
T9287	45235-45239	NNS	denotes	mice
T9288	45240-45244	VBD	denotes	were
T9289	45252-45254	IN	denotes	in
T9290	45255-45258	DT	denotes	the
T9291	45272-45276	NN	denotes	maze
T9292	45259-45265	NNP	denotes	Morris
T9293	45266-45271	NN	denotes	water
T9294	45276-45277	.	denotes	.
T9295	45277-45385	sentence	denotes	In the radial water maze, repetitive swim patterns were noted with no evidence of choice-motivated actions.
T9296	45278-45280	IN	denotes	In
T9297	45334-45339	VBN	denotes	noted
T9298	45281-45284	DT	denotes	the
T9299	45298-45302	NN	denotes	maze
T9300	45285-45291	JJ	denotes	radial
T9301	45292-45297	NN	denotes	water
T9302	45302-45304	,	denotes	,
T9303	45304-45314	JJ	denotes	repetitive
T9304	45320-45328	NNS	denotes	patterns
T9305	45315-45319	NN	denotes	swim
T9306	45329-45333	VBD	denotes	were
T9307	45340-45344	IN	denotes	with
T9308	45345-45347	DT	denotes	no
T9309	45348-45356	NN	denotes	evidence
T9310	45357-45359	IN	denotes	of
T9311	45360-45366	NN	denotes	choice
T9312	45367-45376	VBN	denotes	motivated
T9313	45366-45367	HYPH	denotes	-
T9314	45377-45384	NNS	denotes	actions
T9315	45384-45385	.	denotes	.
T9316	45385-45573	sentence	denotes	Other studies have dealt with similar problems by excluding animals that do not show adequate attention to the task, retaining only those mice that meet certain performance criteria [30].
T9317	45386-45391	JJ	denotes	Other
T9318	45392-45399	NNS	denotes	studies
T9319	45405-45410	VBN	denotes	dealt
T9320	45400-45404	VBP	denotes	have
T9321	45411-45415	IN	denotes	with
T9322	45416-45423	JJ	denotes	similar
T9323	45424-45432	NNS	denotes	problems
T9324	45433-45435	IN	denotes	by
T9325	45436-45445	VBG	denotes	excluding
T9326	45446-45453	NNS	denotes	animals
T9327	45454-45458	WDT	denotes	that
T9328	45466-45470	VB	denotes	show
T9329	45459-45461	VBP	denotes	do
T9330	45462-45465	RB	denotes	not
T9331	45471-45479	JJ	denotes	adequate
T9332	45480-45489	NN	denotes	attention
T9333	45490-45492	IN	denotes	to
T9334	45493-45496	DT	denotes	the
T9335	45497-45501	NN	denotes	task
T9336	45501-45503	,	denotes	,
T9337	45503-45512	VBG	denotes	retaining
T9338	45513-45517	RB	denotes	only
T9339	45524-45528	NNS	denotes	mice
T9340	45518-45523	DT	denotes	those
T9341	45529-45533	WDT	denotes	that
T9342	45534-45538	VBP	denotes	meet
T9343	45539-45546	JJ	denotes	certain
T9344	45559-45567	NNS	denotes	criteria
T9345	45547-45558	NN	denotes	performance
T9346	45568-45569	-LRB-	denotes	[
T9347	45569-45571	CD	denotes	30
T9348	45571-45572	-RRB-	denotes	]
T9349	45572-45573	.	denotes	.
T9350	45573-45674	sentence	denotes	In our case, the penetrance of hyperactivity was close to 100%, leaving us with no testable animals.
T9351	45574-45576	IN	denotes	In
T9352	45619-45622	VBD	denotes	was
T9353	45577-45580	PRP$	denotes	our
T9354	45581-45585	NN	denotes	case
T9355	45585-45587	,	denotes	,
T9356	45587-45590	DT	denotes	the
T9357	45591-45601	NN	denotes	penetrance
T9358	45602-45604	IN	denotes	of
T9359	45605-45618	NN	denotes	hyperactivity
T9360	45623-45628	JJ	denotes	close
T9361	45632-45635	CD	denotes	100
T9362	45629-45631	IN	denotes	to
T9363	45635-45636	NN	denotes	%
T9364	45636-45638	,	denotes	,
T9365	45638-45645	VBG	denotes	leaving
T9366	45646-45648	PRP	denotes	us
T9367	45649-45653	IN	denotes	with
T9368	45654-45656	DT	denotes	no
T9369	45666-45673	NNS	denotes	animals
T9370	45657-45665	JJ	denotes	testable
T9371	45673-45674	.	denotes	.
T9372	45674-45831	sentence	denotes	This phenotype has not affected previous lines of APPswe mice we have produced, such as lines E1–2 or C3–3, that express lower levels of transgenic protein.
T9373	45675-45679	DT	denotes	This
T9374	45680-45689	NN	denotes	phenotype
T9375	45698-45706	VBN	denotes	affected
T9376	45690-45693	VBZ	denotes	has
T9377	45694-45697	RB	denotes	not
T9378	45707-45715	JJ	denotes	previous
T9379	45716-45721	NNS	denotes	lines
T9380	45722-45724	IN	denotes	of
T9381	45725-45731	NN	denotes	APPswe
T9382	45732-45736	NNS	denotes	mice
T9383	45737-45739	PRP	denotes	we
T9384	45745-45753	VBN	denotes	produced
T9385	45740-45744	VBP	denotes	have
T9386	45753-45755	,	denotes	,
T9387	45755-45759	JJ	denotes	such
T9388	45760-45762	IN	denotes	as
T9389	45763-45768	NNS	denotes	lines
T9390	45769-45771	NN	denotes	E1
T9391	45771-45772	HYPH	denotes	–
T9392	45772-45773	CD	denotes	2
T9393	45774-45776	CC	denotes	or
T9394	45777-45779	NN	denotes	C3
T9395	45779-45780	HYPH	denotes	–
T9396	45780-45781	CD	denotes	3
T9397	45781-45783	,	denotes	,
T9398	45783-45787	WDT	denotes	that
T9399	45788-45795	VBP	denotes	express
T9400	45796-45801	JJR	denotes	lower
T9401	45802-45808	NNS	denotes	levels
T9402	45809-45811	IN	denotes	of
T9403	45812-45822	JJ	denotes	transgenic
T9404	45823-45830	NN	denotes	protein
T9405	45830-45831	.	denotes	.
T9406	45831-45978	sentence	denotes	Indeed, in past studies where hyperactivity was not a factor, we established a clear relationship between amyloid load and cognitive ability [31].
T9407	45832-45838	RB	denotes	Indeed
T9408	45897-45908	VBD	denotes	established
T9409	45838-45840	,	denotes	,
T9410	45840-45842	IN	denotes	in
T9411	45843-45847	JJ	denotes	past
T9412	45848-45855	NNS	denotes	studies
T9413	45856-45861	WRB	denotes	where
T9414	45876-45879	VBD	denotes	was
T9415	45862-45875	NN	denotes	hyperactivity
T9416	45880-45883	RB	denotes	not
T9417	45884-45885	DT	denotes	a
T9418	45886-45892	NN	denotes	factor
T9419	45892-45894	,	denotes	,
T9420	45894-45896	PRP	denotes	we
T9421	45909-45910	DT	denotes	a
T9422	45917-45929	NN	denotes	relationship
T9423	45911-45916	JJ	denotes	clear
T9424	45930-45937	IN	denotes	between
T9425	45938-45945	NN	denotes	amyloid
T9426	45946-45950	NN	denotes	load
T9427	45951-45954	CC	denotes	and
T9428	45955-45964	JJ	denotes	cognitive
T9429	45965-45972	NN	denotes	ability
T9430	45973-45974	-LRB-	denotes	[
T9431	45974-45976	CD	denotes	31
T9432	45976-45977	-RRB-	denotes	]
T9433	45977-45978	.	denotes	.
T9434	45978-46232	sentence	denotes	However, in the current study, we feel that although the poor performance of the tTA/APP mice in the maze tests could technically be scored as cognitive impairment, the animals' severe hyperactivity made interpretation of the cognitive tasks impossible.
T9435	45979-45986	RB	denotes	However
T9436	46013-46017	VBP	denotes	feel
T9437	45986-45988	,	denotes	,
T9438	45988-45990	IN	denotes	in
T9439	45991-45994	DT	denotes	the
T9440	46003-46008	NN	denotes	study
T9441	45995-46002	JJ	denotes	current
T9442	46008-46010	,	denotes	,
T9443	46010-46012	PRP	denotes	we
T9444	46018-46022	IN	denotes	that
T9445	46178-46182	VBD	denotes	made
T9446	46023-46031	IN	denotes	although
T9447	46112-46118	VBN	denotes	scored
T9448	46032-46035	DT	denotes	the
T9449	46041-46052	NN	denotes	performance
T9450	46036-46040	JJ	denotes	poor
T9451	46053-46055	IN	denotes	of
T9452	46056-46059	DT	denotes	the
T9453	46068-46072	NNS	denotes	mice
T9454	46060-46063	NN	denotes	tTA
T9455	46064-46067	NN	denotes	APP
T9456	46063-46064	HYPH	denotes	/
T9457	46073-46075	IN	denotes	in
T9458	46076-46079	DT	denotes	the
T9459	46085-46090	NNS	denotes	tests
T9460	46080-46084	NN	denotes	maze
T9461	46091-46096	MD	denotes	could
T9462	46097-46108	RB	denotes	technically
T9463	46109-46111	VB	denotes	be
T9464	46119-46121	IN	denotes	as
T9465	46122-46131	JJ	denotes	cognitive
T9466	46132-46142	NN	denotes	impairment
T9467	46142-46144	,	denotes	,
T9468	46144-46147	DT	denotes	the
T9469	46148-46155	NNS	denotes	animals
T9470	46164-46177	NN	denotes	hyperactivity
T9471	46155-46156	POS	denotes	'
T9472	46157-46163	JJ	denotes	severe
T9473	46183-46197	NN	denotes	interpretation
T9474	46221-46231	JJ	denotes	impossible
T9475	46198-46200	IN	denotes	of
T9476	46201-46204	DT	denotes	the
T9477	46215-46220	NNS	denotes	tasks
T9478	46205-46214	JJ	denotes	cognitive
T9479	46231-46232	.	denotes	.
T9480	46232-46521	sentence	denotes	In order to understand the nature and extent of hyperactivity in the tTA/APP mice, we quantified daily activity levels in double transgenic animals along with their single transgenic and nontransgenic siblings using four-beam frames designed to monitor ambulation within an enclosed cage.
T9481	46233-46235	IN	denotes	In
T9482	46319-46329	VBD	denotes	quantified
T9483	46236-46241	NN	denotes	order
T9484	46242-46244	TO	denotes	to
T9485	46245-46255	VB	denotes	understand
T9486	46256-46259	DT	denotes	the
T9487	46260-46266	NN	denotes	nature
T9488	46267-46270	CC	denotes	and
T9489	46271-46277	NN	denotes	extent
T9490	46278-46280	IN	denotes	of
T9491	46281-46294	NN	denotes	hyperactivity
T9492	46295-46297	IN	denotes	in
T9493	46298-46301	DT	denotes	the
T9494	46310-46314	NNS	denotes	mice
T9495	46302-46305	NN	denotes	tTA
T9496	46306-46309	NN	denotes	APP
T9497	46305-46306	HYPH	denotes	/
T9498	46314-46316	,	denotes	,
T9499	46316-46318	PRP	denotes	we
T9500	46330-46335	JJ	denotes	daily
T9501	46345-46351	NNS	denotes	levels
T9502	46336-46344	NN	denotes	activity
T9503	46352-46354	IN	denotes	in
T9504	46355-46361	JJ	denotes	double
T9505	46362-46372	JJ	denotes	transgenic
T9506	46373-46380	NNS	denotes	animals
T9507	46381-46386	IN	denotes	along
T9508	46387-46391	IN	denotes	with
T9509	46392-46397	PRP$	denotes	their
T9510	46434-46442	NNS	denotes	siblings
T9511	46398-46404	JJ	denotes	single
T9512	46405-46415	JJ	denotes	transgenic
T9513	46416-46419	CC	denotes	and
T9514	46420-46433	JJ	denotes	nontransgenic
T9515	46443-46448	VBG	denotes	using
T9516	46449-46453	CD	denotes	four
T9517	46454-46458	NN	denotes	beam
T9518	46453-46454	HYPH	denotes	-
T9519	46459-46465	NNS	denotes	frames
T9520	46466-46474	VBN	denotes	designed
T9521	46475-46477	TO	denotes	to
T9522	46478-46485	VB	denotes	monitor
T9523	46486-46496	NN	denotes	ambulation
T9524	46497-46503	IN	denotes	within
T9525	46504-46506	DT	denotes	an
T9526	46516-46520	NN	denotes	cage
T9527	46507-46515	VBN	denotes	enclosed
T9528	46520-46521	.	denotes	.
T9529	46521-46678	sentence	denotes	As shown in Figure 7, the double transgenic mice were up to 10-fold more active during the dark phase of the day–night cycle than any of the control groups.
T9530	46522-46524	IN	denotes	As
T9531	46525-46530	VBN	denotes	shown
T9532	46571-46575	VBD	denotes	were
T9533	46531-46533	IN	denotes	in
T9534	46534-46540	NN	denotes	Figure
T9535	46541-46542	CD	denotes	7
T9536	46542-46544	,	denotes	,
T9537	46544-46547	DT	denotes	the
T9538	46566-46570	NNS	denotes	mice
T9539	46548-46554	JJ	denotes	double
T9540	46555-46565	JJ	denotes	transgenic
T9541	46576-46578	IN	denotes	up
T9542	46582-46589	RB	denotes	10-fold
T9543	46579-46581	IN	denotes	to
T9544	46595-46601	JJ	denotes	active
T9545	46590-46594	RBR	denotes	more
T9546	46602-46608	IN	denotes	during
T9547	46609-46612	DT	denotes	the
T9548	46618-46623	NN	denotes	phase
T9549	46613-46617	NN	denotes	dark
T9550	46624-46626	IN	denotes	of
T9551	46627-46630	DT	denotes	the
T9552	46641-46646	NN	denotes	cycle
T9553	46631-46634	NN	denotes	day
T9554	46635-46640	NN	denotes	night
T9555	46634-46635	HYPH	denotes	–
T9556	46647-46651	IN	denotes	than
T9557	46652-46655	DT	denotes	any
T9558	46656-46658	IN	denotes	of
T9559	46659-46662	DT	denotes	the
T9560	46671-46677	NNS	denotes	groups
T9561	46663-46670	NN	denotes	control
T9562	46677-46678	.	denotes	.
T9563	46678-46800	sentence	denotes	Activity levels appeared to follow a relatively normal diurnal cycle, decreasing substantially during the daylight hours.
T9564	46679-46687	NN	denotes	Activity
T9565	46688-46694	NNS	denotes	levels
T9566	46695-46703	VBD	denotes	appeared
T9567	46704-46706	TO	denotes	to
T9568	46707-46713	VB	denotes	follow
T9569	46714-46715	DT	denotes	a
T9570	46742-46747	NN	denotes	cycle
T9571	46716-46726	RB	denotes	relatively
T9572	46727-46733	JJ	denotes	normal
T9573	46734-46741	JJ	denotes	diurnal
T9574	46747-46749	,	denotes	,
T9575	46749-46759	VBG	denotes	decreasing
T9576	46760-46773	RB	denotes	substantially
T9577	46774-46780	IN	denotes	during
T9578	46781-46784	DT	denotes	the
T9579	46794-46799	NNS	denotes	hours
T9580	46785-46793	NN	denotes	daylight
T9581	46799-46800	.	denotes	.
T9582	46800-46908	sentence	denotes	However, even during the light phase, the tTA/APP mice remained many-fold more active than normal controls.
T9583	46801-46808	RB	denotes	However
T9584	46856-46864	VBD	denotes	remained
T9585	46808-46810	,	denotes	,
T9586	46810-46814	RB	denotes	even
T9587	46815-46821	IN	denotes	during
T9588	46822-46825	DT	denotes	the
T9589	46832-46837	NN	denotes	phase
T9590	46826-46831	NN	denotes	light
T9591	46837-46839	,	denotes	,
T9592	46839-46842	DT	denotes	the
T9593	46851-46855	NNS	denotes	mice
T9594	46843-46846	NN	denotes	tTA
T9595	46847-46850	NN	denotes	APP
T9596	46846-46847	HYPH	denotes	/
T9597	46865-46874	RB	denotes	many-fold
T9598	46875-46879	RBR	denotes	more
T9599	46880-46886	JJ	denotes	active
T9600	46887-46891	IN	denotes	than
T9601	46892-46898	JJ	denotes	normal
T9602	46899-46907	NNS	denotes	controls
T9603	46907-46908	.	denotes	.
T9604	46908-47046	sentence	denotes	This behavior was partially, but not consistently, reversed by 1 mo of transgene suppression beginning at 4–5 mo of age (data not shown).
T9605	46909-46913	DT	denotes	This
T9606	46914-46922	NN	denotes	behavior
T9607	46960-46968	VBN	denotes	reversed
T9608	46923-46926	VBD	denotes	was
T9609	46927-46936	RB	denotes	partially
T9610	46936-46938	,	denotes	,
T9611	46938-46941	CC	denotes	but
T9612	46942-46945	RB	denotes	not
T9613	46946-46958	RB	denotes	consistently
T9614	46958-46960	,	denotes	,
T9615	46969-46971	IN	denotes	by
T9616	46972-46973	CD	denotes	1
T9617	46974-46976	NN	denotes	mo
T9618	46977-46979	IN	denotes	of
T9619	46980-46989	NN	denotes	transgene
T9620	46990-47001	NN	denotes	suppression
T9621	47002-47011	VBG	denotes	beginning
T9622	47012-47014	IN	denotes	at
T9623	47015-47016	CD	denotes	4
T9624	47017-47018	CD	denotes	5
T9625	47016-47017	SYM	denotes	–
T9626	47019-47021	NN	denotes	mo
T9627	47022-47024	IN	denotes	of
T9628	47025-47028	NN	denotes	age
T9629	47029-47030	-LRB-	denotes	(
T9630	47039-47044	VBN	denotes	shown
T9631	47030-47034	NNS	denotes	data
T9632	47035-47038	RB	denotes	not
T9633	47044-47045	-RRB-	denotes	)
T9634	47045-47046	.	denotes	.
T9635	47046-47130	sentence	denotes	In contrast, hyperactivity was completely abolished by rearing tTA/APP mice on dox.
T9636	47047-47049	IN	denotes	In
T9637	47089-47098	VBN	denotes	abolished
T9638	47050-47058	NN	denotes	contrast
T9639	47058-47060	,	denotes	,
T9640	47060-47073	NN	denotes	hyperactivity
T9641	47074-47077	VBD	denotes	was
T9642	47078-47088	RB	denotes	completely
T9643	47099-47101	IN	denotes	by
T9644	47102-47109	VBG	denotes	rearing
T9645	47110-47113	NN	denotes	tTA
T9646	47114-47117	NN	denotes	APP
T9647	47113-47114	HYPH	denotes	/
T9648	47118-47122	NNS	denotes	mice
T9649	47123-47125	IN	denotes	on
T9650	47126-47129	NN	denotes	dox
T9651	47129-47130	.	denotes	.
T9652	47130-47231	sentence	denotes	Animals born and raised on dox showed activity levels similar to the untreated controls (Figure 7C).
T9653	47131-47138	NNS	denotes	Animals
T9654	47162-47168	VBD	denotes	showed
T9655	47139-47143	VBN	denotes	born
T9656	47144-47147	CC	denotes	and
T9657	47148-47154	VBN	denotes	raised
T9658	47155-47157	IN	denotes	on
T9659	47158-47161	NN	denotes	dox
T9660	47169-47177	NN	denotes	activity
T9687	47297-47301	JJ	denotes	wild
T9688	47302-47306	NN	denotes	type
T9689	47301-47302	HYPH	denotes	-
T9690	47306-47308	,	denotes	,
T9691	47315-47322	VBN	denotes	altered
T9692	47333-47340	NNS	denotes	rhythms
T9693	47323-47332	JJ	denotes	circadian
T9694	47341-47345	IN	denotes	with
T9695	47346-47349	RB	denotes	far
T9696	47350-47354	JJR	denotes	less
T9697	47355-47366	NN	denotes	distinction
T9698	47367-47374	IN	denotes	between
T9699	47375-47380	PRP$	denotes	their
T9700	47409-47415	NNS	denotes	levels
T9701	47381-47384	NN	denotes	day
T9702	47384-47385	HYPH	denotes	-
T9703	47386-47389	CC	denotes	and
T9704	47390-47399	NN	denotes	nighttime
T9705	47400-47408	NN	denotes	activity
T9706	47415-47416	.	denotes	.
T10541	48164-48166	PRP	denotes	We
T10542	48167-48174	VBP	denotes	present
T10543	48175-48176	DT	denotes	a
T10544	48187-48192	NN	denotes	model
T10545	48177-48180	JJ	denotes	new
T10546	48181-48186	NN	denotes	mouse
T10547	48193-48196	IN	denotes	for
T10548	48197-48199	NN	denotes	AD
T10549	48200-48204	WDT	denotes	that
T10550	48209-48217	VBN	denotes	designed
T10551	48205-48208	VBD	denotes	was
T10552	48218-48220	TO	denotes	to
T10553	48221-48225	VB	denotes	test
T10554	48226-48229	DT	denotes	the
T10555	48230-48242	NNS	denotes	consequences
T10556	48243-48245	IN	denotes	of
T10557	48246-48256	VBG	denotes	inhibiting
T10558	48257-48259	NN	denotes	Aβ
T10559	48260-48270	NN	denotes	production
T10560	48271-48276	IN	denotes	after
T10561	48277-48280	DT	denotes	the
T10562	48281-48286	NN	denotes	onset
T10563	48287-48289	IN	denotes	of
T10564	48290-48297	NN	denotes	amyloid
T10565	48298-48307	NN	denotes	pathology
T10566	48307-48308	.	denotes	.
T10567	48308-48463	sentence	denotes	New lines of transgenic mice were developed for this study that express high levels of APPswe/ind under the control of a tetracycline-responsive promoter.
T10568	48309-48312	JJ	denotes	New
T10569	48313-48318	NNS	denotes	lines
T10570	48343-48352	VBN	denotes	developed
T10571	48319-48321	IN	denotes	of
T10572	48322-48332	JJ	denotes	transgenic
T10573	48333-48337	NNS	denotes	mice
T10574	48338-48342	VBD	denotes	were
T10575	48353-48356	IN	denotes	for
T10576	48357-48361	DT	denotes	this
T10577	48362-48367	NN	denotes	study
T10578	48368-48372	WDT	denotes	that
T10579	48373-48380	VBP	denotes	express
T10580	48381-48385	JJ	denotes	high
T10581	48386-48392	NNS	denotes	levels
T10582	48393-48395	IN	denotes	of
T10583	48396-48402	NN	denotes	APPswe
T10584	48403-48406	NN	denotes	ind
T10585	48402-48403	HYPH	denotes	/
T10586	48407-48412	IN	denotes	under
T10587	48413-48416	DT	denotes	the
T10588	48417-48424	NN	denotes	control
T10589	48425-48427	IN	denotes	of
T10590	48428-48429	DT	denotes	a
T10591	48454-48462	NN	denotes	promoter
T10592	48430-48442	NN	denotes	tetracycline
T10593	48443-48453	JJ	denotes	responsive
T10594	48442-48443	HYPH	denotes	-
T10595	48462-48463	.	denotes	.
T10596	48463-48661	sentence	denotes	We demonstrate that treatment with dox suppresses steady-state levels of both APPswe/ind and its C-terminal fragments, indicating that the mutant proteins have a relatively short half-life in vivo.
T10597	48464-48466	PRP	denotes	We
T10598	48467-48478	VBP	denotes	demonstrate
T10599	48479-48483	IN	denotes	that
T10600	48503-48513	VBZ	denotes	suppresses
T10601	48484-48493	NN	denotes	treatment
T10602	48494-48498	IN	denotes	with
T10603	48499-48502	NN	denotes	dox
T10604	48514-48520	JJ	denotes	steady
T10605	48521-48526	NN	denotes	state
T10606	48520-48521	HYPH	denotes	-
T10607	48527-48533	NNS	denotes	levels
T10608	48534-48536	IN	denotes	of
T10609	48537-48541	CC	denotes	both
T10610	48549-48552	NN	denotes	ind
T10611	48542-48548	NN	denotes	APPswe
T10612	48548-48549	HYPH	denotes	/
T10613	48553-48556	CC	denotes	and
T10614	48557-48560	PRP$	denotes	its
T10615	48572-48581	NNS	denotes	fragments
T10616	48561-48562	NN	denotes	C
T10617	48562-48563	HYPH	denotes	-
T10618	48563-48571	JJ	denotes	terminal
T10619	48581-48583	,	denotes	,
T10620	48583-48593	VBG	denotes	indicating
T10621	48594-48598	IN	denotes	that
T10622	48619-48623	VBP	denotes	have
T10623	48599-48602	DT	denotes	the
T10624	48610-48618	NN	denotes	proteins
T10625	48603-48609	NN	denotes	mutant
T10626	48624-48625	DT	denotes	a
T10627	48648-48652	NN	denotes	life
T10628	48626-48636	RB	denotes	relatively
T10629	48637-48642	JJ	denotes	short
T10630	48643-48647	NN	denotes	half
T10631	48647-48648	HYPH	denotes	-
T10632	48653-48655	FW	denotes	in
T10633	48656-48660	FW	denotes	vivo
T10634	48660-48661	.	denotes	.
T10635	48661-48841	sentence	denotes	Transgenic expression of APPswe/ind and consequent overproduction of Aβ42 cause early-onset amyloid deposition in untreated mice, in which deposits appear as early as 2 mo of age.
T10636	48662-48672	JJ	denotes	Transgenic
T10637	48673-48683	NN	denotes	expression
T10638	48736-48741	VBP	denotes	cause
T10639	48684-48686	IN	denotes	of
T10640	48687-48693	NN	denotes	APPswe
T10641	48694-48697	NN	denotes	ind
T10642	48693-48694	HYPH	denotes	/
T10643	48698-48701	CC	denotes	and
T10644	48702-48712	JJ	denotes	consequent
T10645	48713-48727	NN	denotes	overproduction
T10646	48728-48730	IN	denotes	of
T10647	48731-48735	NN	denotes	Aβ42
T10648	48742-48747	JJ	denotes	early
T10649	48748-48753	NN	denotes	onset
T10650	48747-48748	HYPH	denotes	-
T10651	48762-48772	NN	denotes	deposition
T10652	48754-48761	NN	denotes	amyloid
T10653	48773-48775	IN	denotes	in
T10654	48776-48785	JJ	denotes	untreated
T10655	48786-48790	NNS	denotes	mice
T10656	48790-48792	,	denotes	,
T10657	48792-48794	IN	denotes	in
T10658	48810-48816	VBP	denotes	appear
T10659	48795-48800	WDT	denotes	which
T10660	48801-48809	NNS	denotes	deposits
T10661	48817-48819	RB	denotes	as
T10662	48820-48825	RB	denotes	early
T10663	48826-48828	IN	denotes	as
T10664	48829-48830	CD	denotes	2
T10665	48831-48833	NN	denotes	mo
T10666	48834-48836	IN	denotes	of
T10667	48837-48840	NN	denotes	age
T10668	48840-48841	.	denotes	.
T10669	48841-48990	sentence	denotes	Amyloid burden worsens significantly with age, and by 9 mo, the hippocampus and cortex of untreated mice are largely filled with aggregated peptide.
T10670	48842-48849	NN	denotes	Amyloid
T10671	48850-48856	NN	denotes	burden
T10672	48857-48864	VBZ	denotes	worsens
T10673	48865-48878	RB	denotes	significantly
T10674	48879-48883	IN	denotes	with
T10675	48884-48887	NN	denotes	age
T10676	48887-48889	,	denotes	,
T10677	48889-48892	CC	denotes	and
T10678	48893-48895	IN	denotes	by
T10679	48959-48965	VBN	denotes	filled
T10680	48896-48897	CD	denotes	9
T10681	48898-48900	NN	denotes	mo
T10682	48900-48902	,	denotes	,
T10683	48902-48905	DT	denotes	the
T10684	48906-48917	NN	denotes	hippocampus
T10685	48918-48921	CC	denotes	and
T10686	48922-48928	NN	denotes	cortex
T10687	48929-48931	IN	denotes	of
T10688	48932-48941	JJ	denotes	untreated
T10689	48942-48946	NNS	denotes	mice
T10690	48947-48950	VBP	denotes	are
T10691	48951-48958	RB	denotes	largely
T10692	48966-48970	IN	denotes	with
T10693	48971-48981	VBN	denotes	aggregated
T10694	48982-48989	NN	denotes	peptide
T10695	48989-48990	.	denotes	.
T10696	48990-49103	sentence	denotes	We find that suppression of transgenic APP by more than 95% abruptly halts the progression of amyloid pathology.
T10697	48991-48993	PRP	denotes	We
T10698	48994-48998	VBP	denotes	find
T10699	48999-49003	IN	denotes	that
T10700	49060-49065	VBZ	denotes	halts
T10701	49004-49015	NN	denotes	suppression
T10702	49016-49018	IN	denotes	of
T10703	49019-49029	JJ	denotes	transgenic
T10704	49030-49033	NN	denotes	APP
T10705	49034-49036	IN	denotes	by
T10706	49037-49041	JJR	denotes	more
T10707	49047-49049	CD	denotes	95
T10708	49042-49046	IN	denotes	than
T10709	49049-49050	NN	denotes	%
T10710	49051-49059	RB	denotes	abruptly
T10711	49066-49069	DT	denotes	the
T10712	49070-49081	NN	denotes	progression
T10713	49082-49084	IN	denotes	of
T10714	49085-49092	NN	denotes	amyloid
T10715	49093-49102	NN	denotes	pathology
T10716	49102-49103	.	denotes	.
T10717	49103-49296	sentence	denotes	Importantly, this outcome occurs in animals already harboring considerable amyloid pathology, a situation similar to what might be expected in patients to be treated with secretase inhibitors.
T10718	49104-49115	RB	denotes	Importantly
T10719	49130-49136	VBZ	denotes	occurs
T10720	49115-49117	,	denotes	,
T10721	49117-49121	DT	denotes	this
T10722	49122-49129	NN	denotes	outcome
T10723	49137-49139	IN	denotes	in
T10724	49140-49147	NNS	denotes	animals
T10725	49148-49155	RB	denotes	already
T10726	49156-49165	VBG	denotes	harboring
T10727	49166-49178	JJ	denotes	considerable
T10728	49187-49196	NN	denotes	pathology
T10729	49179-49186	NN	denotes	amyloid
T10730	49196-49198	,	denotes	,
T10731	49198-49199	DT	denotes	a
T10732	49200-49209	NN	denotes	situation
T10733	49210-49217	JJ	denotes	similar
T10734	49218-49220	IN	denotes	to
T10735	49221-49225	WP	denotes	what
T10736	49235-49243	VBN	denotes	expected
T10737	49226-49231	MD	denotes	might
T10738	49232-49234	VB	denotes	be
T10739	49244-49246	IN	denotes	in
T10740	49247-49255	NNS	denotes	patients
T10741	49256-49258	TO	denotes	to
T10742	49262-49269	VBN	denotes	treated
T10743	49259-49261	VB	denotes	be
T10744	49270-49274	IN	denotes	with
T10745	49275-49284	NN	denotes	secretase
T10746	49285-49295	NNS	denotes	inhibitors
T10747	49295-49296	.	denotes	.
T10748	49296-49469	sentence	denotes	Somewhat unexpectedly, we observe no appreciable clearance of deposited amyloid even following periods of transgene suppression equal to the time taken for plaques to form.
T10749	49297-49305	RB	denotes	Somewhat
T10750	49306-49318	RB	denotes	unexpectedly
T10751	49323-49330	VBP	denotes	observe
T10752	49318-49320	,	denotes	,
T10753	49320-49322	PRP	denotes	we
T10754	49331-49333	DT	denotes	no
T10755	49346-49355	NN	denotes	clearance
T10756	49334-49345	JJ	denotes	appreciable
T10757	49356-49358	IN	denotes	of
T10758	49359-49368	VBN	denotes	deposited
T10759	49369-49376	NN	denotes	amyloid
T10760	49377-49381	RB	denotes	even
T10761	49382-49391	VBG	denotes	following
T10762	49392-49399	NNS	denotes	periods
T10763	49400-49402	IN	denotes	of
T10764	49403-49412	NN	denotes	transgene
T10765	49413-49424	NN	denotes	suppression
T10766	49425-49430	JJ	denotes	equal
T10767	49431-49433	IN	denotes	to
T10768	49434-49437	DT	denotes	the
T10769	49438-49442	NN	denotes	time
T10770	49443-49448	VBN	denotes	taken
T10771	49449-49452	IN	denotes	for
T10772	49464-49468	VB	denotes	form
T10773	49453-49460	NNS	denotes	plaques
T10774	49461-49463	TO	denotes	to
T10775	49468-49469	.	denotes	.
T10776	49469-49687	sentence	denotes	This latter finding indicates that compared to other disease-associated protein aggregates such as mutant huntingtin, which clear in less than 3 mo [32], the disaggregation of extracellular amyloid is relatively slow.
T10777	49470-49474	DT	denotes	This
T10778	49482-49489	NN	denotes	finding
T10779	49475-49481	JJ	denotes	latter
T10780	49490-49499	VBZ	denotes	indicates
T10781	49500-49504	IN	denotes	that
T10782	49668-49670	VBZ	denotes	is
T10783	49505-49513	VBN	denotes	compared
T10784	49514-49516	IN	denotes	to
T10785	49517-49522	JJ	denotes	other
T10786	49550-49560	NNS	denotes	aggregates
T10787	49523-49530	NN	denotes	disease
T10788	49531-49541	VBN	denotes	associated
T10789	49530-49531	HYPH	denotes	-
T10790	49542-49549	NN	denotes	protein
T10791	49561-49565	JJ	denotes	such
T10792	49566-49568	IN	denotes	as
T10793	49569-49575	NN	denotes	mutant
T10794	49576-49586	NN	denotes	huntingtin
T10795	49586-49588	,	denotes	,
T10796	49588-49593	WDT	denotes	which
T10797	49594-49599	VBP	denotes	clear
T10798	49600-49602	IN	denotes	in
T10799	49603-49607	JJR	denotes	less
T10800	49613-49614	CD	denotes	3
T10801	49608-49612	IN	denotes	than
T10802	49615-49617	NN	denotes	mo
T10803	49618-49619	-LRB-	denotes	[
T10804	49619-49621	CD	denotes	32
T10805	49621-49622	-RRB-	denotes	]
T10806	49622-49624	,	denotes	,
T10807	49624-49627	DT	denotes	the
T10808	49628-49642	NN	denotes	disaggregation
T10809	49643-49645	IN	denotes	of
T10810	49646-49659	JJ	denotes	extracellular
T10811	49660-49667	NN	denotes	amyloid
T10812	49671-49681	RB	denotes	relatively
T10813	49682-49686	JJ	denotes	slow
T10814	49686-49687	.	denotes	.
T10815	49687-49858	sentence	denotes	Notably, pharmaceutical γ-secretase inhibitors published to date show less strict regulation of Aβ production following chronic administration than we have attained here.
T10816	49688-49695	RB	denotes	Notably
T10817	49753-49757	VBP	denotes	show
T10818	49695-49697	,	denotes	,
T10819	49697-49711	JJ	denotes	pharmaceutical
T10820	49724-49734	NNS	denotes	inhibitors
T10821	49712-49713	SYM	denotes	γ
T10822	49714-49723	NN	denotes	secretase
T10823	49713-49714	HYPH	denotes	-
T10824	49735-49744	VBN	denotes	published
T10825	49745-49747	IN	denotes	to
T10826	49748-49752	NN	denotes	date
T10827	49758-49762	RBR	denotes	less
T10828	49763-49769	JJ	denotes	strict
T10829	49770-49780	NN	denotes	regulation
T10830	49781-49783	IN	denotes	of
T10831	49784-49786	NN	denotes	Aβ
T10832	49787-49797	NN	denotes	production
T10833	49798-49807	VBG	denotes	following
T10834	49808-49815	JJ	denotes	chronic
T10835	49816-49830	NN	denotes	administration
T10836	49831-49835	IN	denotes	than
T10837	49844-49852	VBN	denotes	attained
T10838	49836-49838	PRP	denotes	we
T10839	49839-49843	VBP	denotes	have
T10840	49853-49857	RB	denotes	here
T10841	49857-49858	.	denotes	.
T10842	49858-50068	sentence	denotes	Two independent compounds tested in Tg2576 [33] and TgCRND8 [34] transgenic mice show no more than 85% suppression of Aβ40 levels, and where measured, even less suppression of Aβ42 (approximately 60%) [35,36].
T10843	49859-49862	CD	denotes	Two
T10844	49875-49884	NNS	denotes	compounds
T10845	49863-49874	JJ	denotes	independent
T10846	49940-49944	VBP	denotes	show
T10847	49885-49891	VBN	denotes	tested
T10848	49892-49894	IN	denotes	in
T10849	49895-49901	NN	denotes	Tg2576
T10850	49924-49934	JJ	denotes	transgenic
T10851	49902-49903	-LRB-	denotes	[
T10852	49903-49905	CD	denotes	33
T10853	49905-49906	-RRB-	denotes	]
T10854	49907-49910	CC	denotes	and
T10855	49911-49918	NN	denotes	TgCRND8
T10856	49919-49920	-LRB-	denotes	[
T10857	49920-49922	CD	denotes	34
T10858	49922-49923	-RRB-	denotes	]
T10859	49935-49939	NNS	denotes	mice
T10860	49945-49947	RB	denotes	no
T10861	49958-49960	CD	denotes	85
T10862	49948-49952	JJR	denotes	more
T10863	49953-49957	IN	denotes	than
T10864	49960-49961	NN	denotes	%
T10865	49962-49973	NN	denotes	suppression
T10866	49974-49976	IN	denotes	of
T10867	49977-49981	NN	denotes	Aβ40
T10868	49982-49988	NNS	denotes	levels
T10869	49988-49990	,	denotes	,
T10870	49990-49993	CC	denotes	and
T10871	49994-49999	WRB	denotes	where
T10872	50000-50008	VBN	denotes	measured
T10873	50020-50031	NN	denotes	suppression
T10874	50008-50010	,	denotes	,
T10875	50010-50014	RB	denotes	even
T10876	50015-50019	JJR	denotes	less
T10877	50032-50034	IN	denotes	of
T10878	50035-50039	NN	denotes	Aβ42
T10879	50040-50041	-LRB-	denotes	(
T10880	50057-50058	NN	denotes	%
T10881	50041-50054	RB	denotes	approximately
T10882	50055-50057	CD	denotes	60
T10883	50058-50059	-RRB-	denotes	)
T10884	50060-50061	-LRB-	denotes	[
T10885	50064-50066	CD	denotes	36
T10886	50061-50063	CD	denotes	35
T10887	50063-50064	,	denotes	,
T10888	50066-50067	-RRB-	denotes	]
T10889	50067-50068	.	denotes	.
T10890	50068-50340	sentence	denotes	Thus, even after accounting for the much higher APP expression levels in our mice than in the Tg2576 and TgCRND8 lines, we have achieved better absolute suppression of Aβ production with the tet-off system than is currently possible with published γ-secretase inhibitors.
T10891	50069-50073	RB	denotes	Thus
T10892	50197-50205	VBN	denotes	achieved
T10893	50073-50075	,	denotes	,
T10894	50075-50079	RB	denotes	even
T10895	50080-50085	IN	denotes	after
T10896	50086-50096	VBG	denotes	accounting
T10897	50097-50100	IN	denotes	for
T10898	50101-50104	DT	denotes	the
T10899	50132-50138	NNS	denotes	levels
T10900	50105-50109	RB	denotes	much
T10901	50110-50116	JJR	denotes	higher
T10902	50117-50120	NN	denotes	APP
T10903	50121-50131	NN	denotes	expression
T10904	50139-50141	IN	denotes	in
T10905	50142-50145	PRP$	denotes	our
T10906	50146-50150	NNS	denotes	mice
T10907	50151-50155	IN	denotes	than
T10908	50156-50158	IN	denotes	in
T10909	50159-50162	DT	denotes	the
T10910	50182-50187	NNS	denotes	lines
T10911	50163-50169	NN	denotes	Tg2576
T10912	50170-50173	CC	denotes	and
T10913	50174-50181	NN	denotes	TgCRND8
T10914	50187-50189	,	denotes	,
T10915	50189-50191	PRP	denotes	we
T10916	50192-50196	VBP	denotes	have
T10917	50206-50212	RBR	denotes	better
T10918	50222-50233	NN	denotes	suppression
T10919	50213-50221	JJ	denotes	absolute
T10920	50234-50236	IN	denotes	of
T10921	50237-50239	NN	denotes	Aβ
T10922	50240-50250	NN	denotes	production
T10923	50251-50255	IN	denotes	with
T10924	50256-50259	DT	denotes	the
T10925	50268-50274	NN	denotes	system
T10926	50260-50263	NN	denotes	tet
T10927	50264-50267	NN	denotes	off
T10928	50263-50264	HYPH	denotes	-
T10929	50275-50279	IN	denotes	than
T10930	50280-50282	VBZ	denotes	is
T10931	50283-50292	RB	denotes	currently
T10932	50293-50301	JJ	denotes	possible
T10933	50302-50306	IN	denotes	with
T10934	50307-50316	VBN	denotes	published
T10935	50329-50339	NNS	denotes	inhibitors
T10936	50317-50318	SYM	denotes	γ
T10937	50319-50328	NN	denotes	secretase
T10938	50318-50319	HYPH	denotes	-
T10939	50339-50340	.	denotes	.
T10940	50340-50572	sentence	denotes	Since even the most advanced future pharmaceutical agents are unlikely to attain more complete control of Aβ production than achieved here, this system provides a salient test of therapeutic intervention with Aβ-lowering compounds.
T10941	50341-50346	IN	denotes	Since
T10942	50399-50402	VBP	denotes	are
T10943	50347-50351	RB	denotes	even
T10944	50392-50398	NNS	denotes	agents
T10945	50352-50355	DT	denotes	the
T10946	50356-50360	RBS	denotes	most
T10947	50361-50369	JJ	denotes	advanced
T10948	50370-50376	JJ	denotes	future
T10949	50377-50391	JJ	denotes	pharmaceutical
T10950	50493-50501	VBZ	denotes	provides
T10951	50403-50411	JJ	denotes	unlikely
T10952	50412-50414	TO	denotes	to
T10953	50415-50421	VB	denotes	attain
T10954	50422-50426	RBR	denotes	more
T10955	50427-50435	JJ	denotes	complete
T10956	50436-50443	NN	denotes	control
T10957	50444-50446	IN	denotes	of
T10958	50447-50449	NN	denotes	Aβ
T10959	50450-50460	NN	denotes	production
T10960	50461-50465	IN	denotes	than
T10961	50466-50474	VBN	denotes	achieved
T10962	50475-50479	RB	denotes	here
T10963	50479-50481	,	denotes	,
T10964	50481-50485	DT	denotes	this
T10965	50486-50492	NN	denotes	system
T10966	50502-50503	DT	denotes	a
T10967	50512-50516	NN	denotes	test
T10968	50504-50511	JJ	denotes	salient
T10969	50517-50519	IN	denotes	of
T10970	50520-50531	JJ	denotes	therapeutic
T10971	50532-50544	NN	denotes	intervention
T10972	50545-50549	IN	denotes	with
T10973	50550-50552	NN	denotes	Aβ
T10974	50553-50561	VBG	denotes	lowering
T10975	50552-50553	HYPH	denotes	-
T10976	50562-50571	NNS	denotes	compounds
T10977	50571-50572	.	denotes	.
T10978	50572-50760	sentence	denotes	Although the progression of amyloid deposition was sharply arrested by this approach, we found that a substantial amyloid burden remained even after long periods of transgene suppression.
T10979	50573-50581	IN	denotes	Although
T10980	50632-50640	VBN	denotes	arrested
T10981	50582-50585	DT	denotes	the
T10982	50586-50597	NN	denotes	progression
T10983	50598-50600	IN	denotes	of
T10984	50601-50608	NN	denotes	amyloid
T10985	50609-50619	NN	denotes	deposition
T10986	50620-50623	VBD	denotes	was
T10987	50624-50631	RB	denotes	sharply
T10988	50662-50667	VBD	denotes	found
T10989	50641-50643	IN	denotes	by
T10990	50644-50648	DT	denotes	this
T10991	50649-50657	NN	denotes	approach
T10992	50657-50659	,	denotes	,
T10993	50659-50661	PRP	denotes	we
T10994	50668-50672	IN	denotes	that
T10995	50702-50710	VBD	denotes	remained
T10996	50673-50674	DT	denotes	a
T10997	50695-50701	NN	denotes	burden
T10998	50675-50686	JJ	denotes	substantial
T10999	50687-50694	NN	denotes	amyloid
T11000	50711-50715	RB	denotes	even
T11001	50716-50721	IN	denotes	after
T11002	50722-50726	JJ	denotes	long
T11003	50727-50734	NNS	denotes	periods
T11004	50735-50737	IN	denotes	of
T11005	50738-50747	NN	denotes	transgene
T11006	50748-50759	NN	denotes	suppression
T11007	50759-50760	.	denotes	.
T11008	50760-50919	sentence	denotes	We examined a small number of animals after 12 mo of dox treatment (beginning at 6 mo of age), and found that amyloid deposits were still relatively abundant.
T11009	50761-50763	PRP	denotes	We
T11010	50764-50772	VBD	denotes	examined
T11011	50773-50774	DT	denotes	a
T11012	50781-50787	NN	denotes	number
T11013	50775-50780	JJ	denotes	small
T11014	50788-50790	IN	denotes	of
T11015	50791-50798	NNS	denotes	animals
T11016	50799-50804	IN	denotes	after
T11017	50805-50807	CD	denotes	12
T11018	50808-50810	NN	denotes	mo
T11019	50811-50813	IN	denotes	of
T11020	50814-50817	NN	denotes	dox
T11021	50818-50827	NN	denotes	treatment
T11022	50828-50829	-LRB-	denotes	(
T11023	50829-50838	VBG	denotes	beginning
T11024	50839-50841	IN	denotes	at
T11025	50842-50843	CD	denotes	6
T11026	50844-50846	NN	denotes	mo
T11027	50847-50849	IN	denotes	of
T11028	50850-50853	NN	denotes	age
T11029	50853-50854	-RRB-	denotes	)
T11030	50854-50856	,	denotes	,
T11031	50856-50859	CC	denotes	and
T11032	50860-50865	VBD	denotes	found
T11033	50866-50870	IN	denotes	that
T11034	50888-50892	VBD	denotes	were
T11035	50871-50878	NN	denotes	amyloid
T11036	50879-50887	NNS	denotes	deposits
T11037	50893-50898	RB	denotes	still
T11038	50899-50909	RB	denotes	relatively
T11039	50910-50918	JJ	denotes	abundant
T11040	50918-50919	.	denotes	.
T11041	50919-50963	sentence	denotes	Longer-term treatments are now in progress.
T11042	50920-50926	JJ	denotes	Longer
T11043	50927-50931	NN	denotes	term
T11044	50926-50927	HYPH	denotes	-
T11045	50932-50942	NNS	denotes	treatments
T11046	50943-50946	VBP	denotes	are
T11047	50947-50950	RB	denotes	now
T11048	50951-50953	IN	denotes	in
T11049	50954-50962	NN	denotes	progress
T11050	50962-50963	.	denotes	.
T11051	50963-51266	sentence	denotes	At the latest treatment interval analyzed by ELISA, animals administered dox for 6 mo showed elevated levels of PBS-soluble Aβ (see Figure 5) that could be interpreted as an indication that the plaques are slowly releasing peptide (or oligomeric Aβ) into the soluble pool and might eventually dissolve.
T11052	50964-50966	IN	denotes	At
T11053	51050-51056	VBD	denotes	showed
T11054	50967-50970	DT	denotes	the
T11055	50988-50996	NN	denotes	interval
T11056	50971-50977	JJS	denotes	latest
T11057	50978-50987	NN	denotes	treatment
T11058	50997-51005	VBN	denotes	analyzed
T11059	51006-51008	IN	denotes	by
T11060	51009-51014	NN	denotes	ELISA
T11061	51014-51016	,	denotes	,
T11062	51016-51023	NNS	denotes	animals
T11063	51024-51036	VBN	denotes	administered
T11064	51037-51040	NN	denotes	dox
T11065	51041-51044	IN	denotes	for
T11066	51045-51046	CD	denotes	6
T11067	51047-51049	NN	denotes	mo
T11068	51057-51065	VBN	denotes	elevated
T11069	51066-51072	NNS	denotes	levels
T11070	51073-51075	IN	denotes	of
T11071	51076-51079	NN	denotes	PBS
T11072	51080-51087	JJ	denotes	soluble
T11073	51079-51080	HYPH	denotes	-
T11074	51088-51090	NN	denotes	Aβ
T11075	51091-51092	-LRB-	denotes	(
T11076	51092-51095	VB	denotes	see
T11077	51096-51102	NN	denotes	Figure
T11078	51103-51104	CD	denotes	5
T11079	51104-51105	-RRB-	denotes	)
T11080	51106-51110	WDT	denotes	that
T11081	51120-51131	VBN	denotes	interpreted
T11082	51111-51116	MD	denotes	could
T11083	51117-51119	VB	denotes	be
T11084	51132-51134	IN	denotes	as
T11085	51135-51137	DT	denotes	an
T11086	51138-51148	NN	denotes	indication
T11087	51149-51153	IN	denotes	that
T11088	51177-51186	VBG	denotes	releasing
T11089	51154-51157	DT	denotes	the
T11090	51158-51165	NNS	denotes	plaques
T11091	51166-51169	VBP	denotes	are
T11092	51170-51176	RB	denotes	slowly
T11093	51187-51194	NN	denotes	peptide
T11094	51195-51196	-LRB-	denotes	(
T11095	51196-51198	CC	denotes	or
T11096	51199-51209	JJ	denotes	oligomeric
T11097	51210-51212	NN	denotes	Aβ
T11098	51212-51213	-RRB-	denotes	)
T11099	51214-51218	IN	denotes	into
T11100	51219-51222	DT	denotes	the
T11101	51231-51235	NN	denotes	pool
T11102	51223-51230	JJ	denotes	soluble
T11103	51236-51239	CC	denotes	and
T11104	51240-51245	MD	denotes	might
T11105	51257-51265	VB	denotes	dissolve
T11106	51246-51256	RB	denotes	eventually
T11107	51265-51266	.	denotes	.
T11108	51266-51471	sentence	denotes	Whether inhibiting Aβ production longer than 6 (or 12) mo may ultimately result in clearance of amyloid is under investigation; unfortunately, the life span of the model eventually limits this experiment.
T11109	51267-51274	IN	denotes	Whether
T11110	51340-51346	VB	denotes	result
T11111	51275-51285	VBG	denotes	inhibiting
T11112	51286-51288	NN	denotes	Aβ
T11113	51289-51299	NN	denotes	production
T11114	51300-51306	RBR	denotes	longer
T11115	51307-51311	IN	denotes	than
T11116	51312-51313	CD	denotes	6
T11117	51322-51324	NN	denotes	mo
T11118	51314-51315	-LRB-	denotes	(
T11119	51315-51317	CC	denotes	or
T11120	51318-51320	CD	denotes	12
T11121	51320-51321	-RRB-	denotes	)
T11122	51325-51328	MD	denotes	may
T11123	51329-51339	RB	denotes	ultimately
T11124	51371-51373	VBZ	denotes	is
T11125	51347-51349	IN	denotes	in
T11126	51350-51359	NN	denotes	clearance
T11127	51360-51362	IN	denotes	of
T11128	51363-51370	NN	denotes	amyloid
T11129	51448-51454	VBZ	denotes	limits
T11130	51374-51379	IN	denotes	under
T11131	51380-51393	NN	denotes	investigation
T11132	51393-51394	:	denotes	;
T11133	51395-51408	RB	denotes	unfortunately
T11134	51408-51410	,	denotes	,
T11135	51410-51413	DT	denotes	the
T11136	51419-51423	NN	denotes	span
T11137	51414-51418	NN	denotes	life
T11138	51424-51426	IN	denotes	of
T11139	51427-51430	DT	denotes	the
T11140	51431-51436	NN	denotes	model
T11141	51437-51447	RB	denotes	eventually
T11142	51455-51459	DT	denotes	this
T11143	51460-51470	NN	denotes	experiment
T11144	51470-51471	.	denotes	.
T11145	51471-51595	sentence	denotes	Therapeutics used in humans will have considerably more time to act than is possible within the life span of rodent models.
T11146	51472-51484	NNS	denotes	Therapeutics
T11147	51505-51509	VB	denotes	have
T11148	51485-51489	VBN	denotes	used
T11149	51490-51492	IN	denotes	in
T11150	51493-51499	NNS	denotes	humans
T11151	51500-51504	MD	denotes	will
T11152	51510-51522	RB	denotes	considerably
T11153	51523-51527	JJR	denotes	more
T11154	51528-51532	NN	denotes	time
T11155	51533-51535	TO	denotes	to
T11156	51536-51539	VB	denotes	act
T11157	51540-51544	IN	denotes	than
T11158	51545-51547	VBZ	denotes	is
T11159	51548-51556	JJ	denotes	possible
T11160	51557-51563	IN	denotes	within
T11161	51564-51567	DT	denotes	the
T11162	51573-51577	NN	denotes	span
T11163	51568-51572	NN	denotes	life
T11164	51578-51580	IN	denotes	of
T11165	51581-51587	NN	denotes	rodent
T11166	51588-51594	NNS	denotes	models
T11167	51594-51595	.	denotes	.
T11168	51595-51685	sentence	denotes	Long-term treatments would certainly be possible and could be a key to effective therapy.
T11169	51596-51600	JJ	denotes	Long
T11170	51601-51605	NN	denotes	term
T11171	51600-51601	HYPH	denotes	-
T11172	51606-51616	NNS	denotes	treatments
T11173	51633-51635	VB	denotes	be
T11174	51617-51622	MD	denotes	would
T11175	51623-51632	RB	denotes	certainly
T11176	51636-51644	JJ	denotes	possible
T11177	51645-51648	CC	denotes	and
T11178	51649-51654	MD	denotes	could
T11179	51655-51657	VB	denotes	be
T11180	51658-51659	DT	denotes	a
T11181	51660-51663	NN	denotes	key
T11182	51664-51666	IN	denotes	to
T11183	51667-51676	JJ	denotes	effective
T11184	51677-51684	NN	denotes	therapy
T11185	51684-51685	.	denotes	.
T11186	51685-51982	sentence	denotes	Overall, however, we interpret our findings as evidence that AD therapies that significantly lower the production of Aβ (by either inhibiting secretase activity or inhibiting APP expression) may not quickly reverse preexisting pathology, but should effectively halt further deposition of amyloid.
T11187	51686-51693	RB	denotes	Overall
T11188	51707-51716	VBP	denotes	interpret
T11189	51693-51695	,	denotes	,
T11190	51695-51702	RB	denotes	however
T11191	51702-51704	,	denotes	,
T11192	51704-51706	PRP	denotes	we
T11193	51717-51720	PRP$	denotes	our
T11194	51721-51729	NNS	denotes	findings
T11195	51730-51732	IN	denotes	as
T11196	51733-51741	NN	denotes	evidence
T11197	51742-51746	IN	denotes	that
T11198	51893-51900	VB	denotes	reverse
T11199	51747-51749	NN	denotes	AD
T11200	51750-51759	NNS	denotes	therapies
T11201	51760-51764	WDT	denotes	that
T11202	51779-51784	VBP	denotes	lower
T11203	51765-51778	RB	denotes	significantly
T11204	51785-51788	DT	denotes	the
T11205	51789-51799	NN	denotes	production
T11206	51800-51802	IN	denotes	of
T11207	51803-51805	NN	denotes	Aβ
T11208	51806-51807	-LRB-	denotes	(
T11209	51807-51809	IN	denotes	by
T11210	51810-51816	CC	denotes	either
T11211	51817-51827	VBG	denotes	inhibiting
T11212	51828-51837	NN	denotes	secretase
T11213	51838-51846	NN	denotes	activity
T11214	51847-51849	CC	denotes	or
T11215	51850-51860	VBG	denotes	inhibiting
T11216	51861-51864	NN	denotes	APP
T11217	51865-51875	NN	denotes	expression
T11218	51875-51876	-RRB-	denotes	)
T11219	51877-51880	MD	denotes	may
T11220	51881-51884	RB	denotes	not
T11221	51885-51892	RB	denotes	quickly
T11222	51901-51912	JJ	denotes	preexisting
T11223	51913-51922	NN	denotes	pathology
T11224	51922-51924	,	denotes	,
T11225	51924-51927	CC	denotes	but
T11226	51928-51934	MD	denotes	should
T11227	51947-51951	VB	denotes	halt
T11228	51935-51946	RB	denotes	effectively
T11229	51952-51959	JJ	denotes	further
T11230	51960-51970	NN	denotes	deposition
T11231	51971-51973	IN	denotes	of
T11232	51974-51981	NN	denotes	amyloid
T11233	51981-51982	.	denotes	.
T11234	51982-52300	sentence	denotes	In interpreting our study, it should be remembered that the earliest plaques to appear in these mice, like other APP transgenics harboring the Swedish mutation [27], are predominantly fibrillar deposits, which may be less tractable than the diffuse aggregates thought to come first in the course of the human disease.
T11235	51983-51985	IN	denotes	In
T11236	52023-52033	VBN	denotes	remembered
T11237	51986-51998	VBG	denotes	interpreting
T11238	51999-52002	PRP$	denotes	our
T11239	52003-52008	NN	denotes	study
T11240	52008-52010	,	denotes	,
T11241	52010-52012	PRP	denotes	it
T11242	52013-52019	MD	denotes	should
T11243	52020-52022	VB	denotes	be
T11244	52034-52038	IN	denotes	that
T11245	52149-52152	VBP	denotes	are
T11246	52039-52042	DT	denotes	the
T11247	52052-52059	NNS	denotes	plaques
T11248	52043-52051	JJS	denotes	earliest
T11249	52060-52062	TO	denotes	to
T11250	52063-52069	VB	denotes	appear
T11251	52070-52072	IN	denotes	in
T11252	52073-52078	DT	denotes	these
T11253	52079-52083	NNS	denotes	mice
T11254	52083-52085	,	denotes	,
T11255	52085-52089	IN	denotes	like
T11256	52090-52095	JJ	denotes	other
T11257	52100-52111	NNS	denotes	transgenics
T11258	52096-52099	NN	denotes	APP
T11259	52112-52121	VBG	denotes	harboring
T11260	52122-52125	DT	denotes	the
T11261	52134-52142	NN	denotes	mutation
T11262	52126-52133	JJ	denotes	Swedish
T11263	52143-52144	-LRB-	denotes	[
T11264	52144-52146	CD	denotes	27
T11265	52146-52147	-RRB-	denotes	]
T11266	52147-52149	,	denotes	,
T11267	52153-52166	RB	denotes	predominantly
T11268	52167-52176	JJ	denotes	fibrillar
T11269	52177-52185	NNS	denotes	deposits
T11270	52185-52187	,	denotes	,
T11271	52187-52192	WDT	denotes	which
T11272	52197-52199	VB	denotes	be
T11273	52193-52196	MD	denotes	may
T11274	52200-52204	RBR	denotes	less
T11275	52205-52214	JJ	denotes	tractable
T11276	52215-52219	IN	denotes	than
T11277	52220-52223	DT	denotes	the
T11278	52232-52242	NNS	denotes	aggregates
T11279	52224-52231	JJ	denotes	diffuse
T11280	52243-52250	VBN	denotes	thought
T11281	52251-52253	TO	denotes	to
T11282	52254-52258	VB	denotes	come
T11283	52259-52264	RB	denotes	first
T11284	52265-52267	IN	denotes	in
T11285	52268-52271	DT	denotes	the
T11286	52272-52278	NN	denotes	course
T11287	52279-52281	IN	denotes	of
T11288	52282-52285	DT	denotes	the
T11289	52292-52299	NN	denotes	disease
T11290	52286-52291	JJ	denotes	human
T11291	52299-52300	.	denotes	.
T11292	52300-52461	sentence	denotes	However, our data suggest that once diffuse deposits are formed in these mice, they are no more easily cleared in our system than cored plaques (see Figure S4).
T11293	52301-52308	RB	denotes	However
T11294	52319-52326	VBP	denotes	suggest
T11295	52308-52310	,	denotes	,
T11296	52310-52313	PRP$	denotes	our
T11297	52314-52318	NNS	denotes	data
T11298	52327-52331	IN	denotes	that
T11299	52404-52411	VBN	denotes	cleared
T11300	52332-52336	IN	denotes	once
T11301	52358-52364	VBN	denotes	formed
T11302	52337-52344	JJ	denotes	diffuse
T11303	52345-52353	NNS	denotes	deposits
T11304	52354-52357	VBP	denotes	are
T11305	52365-52367	IN	denotes	in
T11306	52368-52373	DT	denotes	these
T11307	52374-52378	NNS	denotes	mice
T11308	52378-52380	,	denotes	,
T11309	52380-52384	PRP	denotes	they
T11310	52385-52388	VBP	denotes	are
T11311	52389-52391	RB	denotes	no
T11312	52392-52396	RBR	denotes	more
T11313	52397-52403	RB	denotes	easily
T11314	52412-52414	IN	denotes	in
T11315	52415-52418	PRP$	denotes	our
T11316	52419-52425	NN	denotes	system
T11317	52426-52430	IN	denotes	than
T11318	52431-52436	VBN	denotes	cored
T11319	52437-52444	NNS	denotes	plaques
T11320	52445-52446	-LRB-	denotes	(
T11321	52446-52449	VB	denotes	see
T11322	52450-52456	NN	denotes	Figure
T11323	52457-52459	NN	denotes	S4
T11324	52459-52460	-RRB-	denotes	)
T11325	52460-52461	.	denotes	.
T11326	52461-52637	sentence	denotes	An additional consideration we recognize is that a small amount of transgene expression continues in the presence of dox and that endogenous mouse Aβ continues to be produced.
T11327	52462-52464	DT	denotes	An
T11328	52476-52489	NN	denotes	consideration
T11329	52465-52475	JJ	denotes	additional
T11330	52503-52505	VBZ	denotes	is
T11331	52490-52492	PRP	denotes	we
T11332	52493-52502	VBP	denotes	recognize
T11333	52506-52510	IN	denotes	that
T11334	52550-52559	VBZ	denotes	continues
T11335	52511-52512	DT	denotes	a
T11336	52519-52525	NN	denotes	amount
T11337	52513-52518	JJ	denotes	small
T11338	52526-52528	IN	denotes	of
T11339	52529-52538	NN	denotes	transgene
T11340	52539-52549	NN	denotes	expression
T11341	52560-52562	IN	denotes	in
T11342	52563-52566	DT	denotes	the
T11343	52567-52575	NN	denotes	presence
T11344	52576-52578	IN	denotes	of
T11345	52579-52582	NN	denotes	dox
T11346	52583-52586	CC	denotes	and
T11347	52587-52591	IN	denotes	that
T11348	52612-52621	VBZ	denotes	continues
T11349	52592-52602	JJ	denotes	endogenous
T11350	52609-52611	NN	denotes	Aβ
T11351	52603-52608	NN	denotes	mouse
T11352	52622-52624	TO	denotes	to
T11353	52628-52636	VBN	denotes	produced
T11354	52625-52627	VB	denotes	be
T11355	52636-52637	.	denotes	.
T11356	52637-52782	sentence	denotes	It is possible that the combined low levels of endogenous mouse Aβ and nonsuppressed human peptide are sufficient to maintain existing deposits.
T11357	52638-52640	PRP	denotes	It
T11358	52641-52643	VBZ	denotes	is
T11359	52644-52652	JJ	denotes	possible
T11360	52653-52657	IN	denotes	that
T11361	52737-52740	VBP	denotes	are
T11362	52658-52661	DT	denotes	the
T11363	52675-52681	NNS	denotes	levels
T11364	52662-52670	VBN	denotes	combined
T11365	52671-52674	JJ	denotes	low
T11366	52682-52684	IN	denotes	of
T11367	52685-52695	JJ	denotes	endogenous
T11368	52702-52704	NN	denotes	Aβ
T11369	52696-52701	NN	denotes	mouse
T11370	52729-52736	NN	denotes	peptide
T11371	52705-52708	CC	denotes	and
T11372	52709-52722	JJ	denotes	nonsuppressed
T11373	52723-52728	JJ	denotes	human
T11374	52741-52751	JJ	denotes	sufficient
T11375	52752-52754	TO	denotes	to
T11376	52755-52763	VB	denotes	maintain
T11377	52764-52772	VBG	denotes	existing
T11378	52773-52781	NNS	denotes	deposits
T11379	52781-52782	.	denotes	.
T11380	52782-52987	sentence	denotes	However, these low levels of peptide are not sufficient to induce new amyloid formation, as CaMKIIα-tTA × tetAPPswe/ind mice raised on dox for up to a year do not develop amyloid lesions (data not shown).
T11381	52783-52790	RB	denotes	However
T11382	52820-52823	VBP	denotes	are
T11383	52790-52792	,	denotes	,
T11384	52792-52797	DT	denotes	these
T11385	52802-52808	NNS	denotes	levels
T11386	52798-52801	JJ	denotes	low
T11387	52809-52811	IN	denotes	of
T11388	52812-52819	NN	denotes	peptide
T11389	52824-52827	RB	denotes	not
T11390	52828-52838	JJ	denotes	sufficient
T11391	52839-52841	TO	denotes	to
T11392	52842-52848	VB	denotes	induce
T11393	52849-52852	JJ	denotes	new
T11394	52861-52870	NN	denotes	formation
T11395	52853-52860	NN	denotes	amyloid
T11396	52870-52872	,	denotes	,
T11397	52872-52874	IN	denotes	as
T11398	52946-52953	VB	denotes	develop
T11399	52875-52882	NN	denotes	CaMKIIα
T11400	52899-52902	NN	denotes	ind
T11401	52882-52883	HYPH	denotes	-
T11402	52883-52886	NN	denotes	tTA
T11403	52887-52888	SYM	denotes	×
T11404	52889-52898	NN	denotes	tetAPPswe
T11405	52898-52899	HYPH	denotes	/
T11406	52903-52907	NNS	denotes	mice
T11407	52908-52914	VBN	denotes	raised
T11408	52915-52917	IN	denotes	on
T11409	52918-52921	NN	denotes	dox
T11410	52922-52925	IN	denotes	for
T11411	52926-52928	IN	denotes	up
T11412	52932-52933	DT	denotes	a
T11413	52929-52931	IN	denotes	to
T11414	52934-52938	NN	denotes	year
T11415	52939-52941	VBP	denotes	do
T11416	52942-52945	RB	denotes	not
T11417	52954-52961	NN	denotes	amyloid
T11418	52962-52969	NNS	denotes	lesions
T11419	52970-52971	-LRB-	denotes	(
T11420	52980-52985	VBN	denotes	shown
T11421	52971-52975	NNS	denotes	data
T11422	52976-52979	RB	denotes	not
T11423	52985-52986	-RRB-	denotes	)
T11424	52986-52987	.	denotes	.
T11425	52987-53167	sentence	denotes	It is also clear that in this genetic system, we have raised the production of Aβ to levels not found in humans to accelerate pathology into an experimentally feasible time frame.
T11426	52988-52990	PRP	denotes	It
T11427	52991-52993	VBZ	denotes	is
T11428	52994-52998	RB	denotes	also
T11429	52999-53004	JJ	denotes	clear
T11430	53005-53009	IN	denotes	that
T11431	53042-53048	VBN	denotes	raised
T11432	53010-53012	IN	denotes	in
T11433	53013-53017	DT	denotes	this
T11434	53026-53032	NN	denotes	system
T11435	53018-53025	JJ	denotes	genetic
T11436	53032-53034	,	denotes	,
T11437	53034-53036	PRP	denotes	we
T11438	53037-53041	VBP	denotes	have
T11439	53049-53052	DT	denotes	the
T11440	53053-53063	NN	denotes	production
T11441	53064-53066	IN	denotes	of
T11442	53067-53069	NN	denotes	Aβ
T11443	53070-53072	IN	denotes	to
T11444	53073-53079	NNS	denotes	levels
T11445	53080-53083	RB	denotes	not
T11446	53084-53089	VBN	denotes	found
T11447	53090-53092	IN	denotes	in
T11448	53093-53099	NNS	denotes	humans
T11449	53100-53102	TO	denotes	to
T11450	53103-53113	VB	denotes	accelerate
T11451	53114-53123	NN	denotes	pathology
T11452	53124-53128	IN	denotes	into
T11453	53129-53131	DT	denotes	an
T11454	53161-53166	NN	denotes	frame
T11455	53132-53146	RB	denotes	experimentally
T11456	53147-53155	JJ	denotes	feasible
T11457	53156-53160	NN	denotes	time
T11458	53166-53167	.	denotes	.
T11459	53167-53426	sentence	denotes	This system allowed us to create an approximately 20-fold differential between APP/Aβ synthetic rates before and after treatment, yet the in vivo equilibrium between aggregated and disaggregated states of Aβ still favored the maintenance of amyloid deposits.
T11460	53168-53172	DT	denotes	This
T11461	53173-53179	NN	denotes	system
T11462	53180-53187	VBD	denotes	allowed
T11463	53382-53389	VBD	denotes	favored
T11464	53188-53190	PRP	denotes	us
T11465	53194-53200	VB	denotes	create
T11466	53191-53193	TO	denotes	to
T11467	53201-53203	DT	denotes	an
T11468	53226-53238	NN	denotes	differential
T11469	53204-53217	RB	denotes	approximately
T11470	53218-53225	JJ	denotes	20-fold
T11471	53239-53246	IN	denotes	between
T11472	53247-53250	NN	denotes	APP
T11473	53251-53253	NN	denotes	Aβ
T11474	53250-53251	HYPH	denotes	/
T11475	53264-53269	NNS	denotes	rates
T11476	53254-53263	JJ	denotes	synthetic
T11477	53270-53276	IN	denotes	before
T11478	53277-53280	CC	denotes	and
T11479	53281-53286	IN	denotes	after
T11480	53287-53296	NN	denotes	treatment
T11481	53296-53298	,	denotes	,
T11482	53298-53301	RB	denotes	yet
T11483	53302-53305	DT	denotes	the
T11484	53314-53325	NN	denotes	equilibrium
T11485	53306-53308	FW	denotes	in
T11486	53309-53313	FW	denotes	vivo
T11487	53326-53333	IN	denotes	between
T11488	53334-53344	VBN	denotes	aggregated
T11489	53363-53369	NNS	denotes	states
T11490	53345-53348	CC	denotes	and
T11491	53349-53362	VBN	denotes	disaggregated
T11492	53370-53372	IN	denotes	of
T11493	53373-53375	NN	denotes	Aβ
T11494	53376-53381	RB	denotes	still
T11495	53390-53393	DT	denotes	the
T11496	53394-53405	NN	denotes	maintenance
T11497	53406-53408	IN	denotes	of
T11498	53409-53416	NN	denotes	amyloid
T11499	53417-53425	NNS	denotes	deposits
T11500	53425-53426	.	denotes	.
T11501	53426-53567	sentence	denotes	In our opinion, it seems unlikely that amyloid deposits in human brain would be inherently any less stable than those formed in mouse brain.
T11502	53427-53429	IN	denotes	In
T11503	53446-53451	VBZ	denotes	seems
T11504	53430-53433	PRP$	denotes	our
T11505	53434-53441	NN	denotes	opinion
T11506	53441-53443	,	denotes	,
T11507	53443-53445	PRP	denotes	it
T11508	53452-53460	JJ	denotes	unlikely
T11509	53461-53465	IN	denotes	that
T11510	53504-53506	VB	denotes	be
T11511	53466-53473	NN	denotes	amyloid
T11512	53474-53482	NNS	denotes	deposits
T11513	53483-53485	IN	denotes	in
T11514	53486-53491	JJ	denotes	human
T11515	53492-53497	NN	denotes	brain
T11516	53498-53503	MD	denotes	would
T11517	53507-53517	RB	denotes	inherently
T11518	53518-53521	DT	denotes	any
T11519	53527-53533	JJ	denotes	stable
T11520	53522-53526	RBR	denotes	less
T11521	53534-53538	IN	denotes	than
T11522	53539-53544	DT	denotes	those
T11523	53545-53551	VBN	denotes	formed
T11524	53552-53554	IN	denotes	in
T11525	53555-53560	NN	denotes	mouse
T11526	53561-53566	NN	denotes	brain
T11527	53566-53567	.	denotes	.
T11528	53567-53707	sentence	denotes	However, the human brain may harbor clearance mechanisms not shared by mice that would allow more efficient removal of preexisting amyloid.
T11529	53568-53575	RB	denotes	However
T11530	53597-53603	VB	denotes	harbor
T11531	53575-53577	,	denotes	,
T11532	53577-53580	DT	denotes	the
T11533	53587-53592	NN	denotes	brain
T11534	53581-53586	JJ	denotes	human
T11535	53593-53596	MD	denotes	may
T11536	53604-53613	NN	denotes	clearance
T11537	53614-53624	NNS	denotes	mechanisms
T11538	53625-53628	RB	denotes	not
T11539	53629-53635	VBN	denotes	shared
T11540	53636-53638	IN	denotes	by
T11541	53639-53643	NNS	denotes	mice
T11542	53644-53648	WDT	denotes	that
T11543	53655-53660	VB	denotes	allow
T11544	53649-53654	MD	denotes	would
T11545	53661-53665	RBR	denotes	more
T11546	53666-53675	JJ	denotes	efficient
T11547	53676-53683	NN	denotes	removal
T11548	53684-53686	IN	denotes	of
T11549	53687-53698	VBG	denotes	preexisting
T11550	53699-53706	NN	denotes	amyloid
T11551	53706-53707	.	denotes	.
T11552	53707-53862	sentence	denotes	One potential mechanism by which amyloid may be more efficiently removed in the human disease than in the mouse models is through microglial phagocytosis.
T11553	53708-53711	CD	denotes	One
T11554	53722-53731	NN	denotes	mechanism
T11555	53712-53721	JJ	denotes	potential
T11556	53827-53829	VBZ	denotes	is
T11557	53732-53734	IN	denotes	by
T11558	53773-53780	VBN	denotes	removed
T11559	53735-53740	WDT	denotes	which
T11560	53741-53748	NN	denotes	amyloid
T11561	53749-53752	MD	denotes	may
T11562	53753-53755	VB	denotes	be
T11563	53756-53760	RBR	denotes	more
T11564	53761-53772	RB	denotes	efficiently
T11565	53781-53783	IN	denotes	in
T11566	53784-53787	DT	denotes	the
T11567	53794-53801	NN	denotes	disease
T11568	53788-53793	JJ	denotes	human
T11569	53802-53806	IN	denotes	than
T11570	53807-53809	IN	denotes	in
T11571	53810-53813	DT	denotes	the
T11572	53820-53826	NNS	denotes	models
T11573	53814-53819	NN	denotes	mouse
T11574	53830-53837	IN	denotes	through
T11575	53838-53848	JJ	denotes	microglial
T11576	53849-53861	NN	denotes	phagocytosis
T11577	53861-53862	.	denotes	.
T11578	53862-54003	sentence	denotes	Resident microglia in transgenic mouse models localize to tissue surrounding plaques but show little evidence of amyloid engulfment [37–40].
T11579	53863-53871	JJ	denotes	Resident
T11580	53872-53881	NNS	denotes	microglia
T11581	53909-53917	VBP	denotes	localize
T11582	53882-53884	IN	denotes	in
T11583	53885-53895	JJ	denotes	transgenic
T11584	53902-53908	NNS	denotes	models
T11585	53896-53901	NN	denotes	mouse
T11586	53918-53920	IN	denotes	to
T11587	53921-53927	NN	denotes	tissue
T11588	53928-53939	VBG	denotes	surrounding
T11589	53940-53947	NNS	denotes	plaques
T11590	53948-53951	CC	denotes	but
T11591	53952-53956	VBP	denotes	show
T11592	53957-53963	JJ	denotes	little
T11593	53964-53972	NN	denotes	evidence
T11594	53973-53975	IN	denotes	of
T11595	53976-53983	NN	denotes	amyloid
T11596	53984-53994	NN	denotes	engulfment
T11597	53995-53996	-LRB-	denotes	[
T11598	53996-53998	CD	denotes	37
T11599	53998-53999	SYM	denotes	–
T11600	53999-54001	CD	denotes	40
T11601	54001-54002	-RRB-	denotes	]
T11602	54002-54003	.	denotes	.
T11603	54003-54161	sentence	denotes	In contrast, microglia surrounding amyloid plaques in human brain show a much higher state of activation with greater expression of complement receptor [40].
T11604	54004-54006	IN	denotes	In
T11605	54070-54074	VBP	denotes	show
T11606	54007-54015	NN	denotes	contrast
T11607	54015-54017	,	denotes	,
T11608	54017-54026	NNS	denotes	microglia
T11609	54027-54038	VBG	denotes	surrounding
T11610	54039-54046	NN	denotes	amyloid
T11611	54047-54054	NNS	denotes	plaques
T11612	54055-54057	IN	denotes	in
T11613	54058-54063	JJ	denotes	human
T11614	54064-54069	NN	denotes	brain
T11615	54075-54076	DT	denotes	a
T11616	54089-54094	NN	denotes	state
T11617	54077-54081	RB	denotes	much
T11618	54082-54088	JJR	denotes	higher
T11619	54095-54097	IN	denotes	of
T11620	54098-54108	NN	denotes	activation
T11621	54109-54113	IN	denotes	with
T11622	54114-54121	JJR	denotes	greater
T11623	54122-54132	NN	denotes	expression
T11624	54133-54135	IN	denotes	of
T11625	54136-54146	NN	denotes	complement
T11626	54147-54155	NN	denotes	receptor
T11627	54156-54157	-LRB-	denotes	[
T11628	54157-54159	CD	denotes	40
T11629	54159-54160	-RRB-	denotes	]
T11630	54160-54161	.	denotes	.
T11631	54161-54274	sentence	denotes	Thus, the role of microglia in amyloid metabolism is minor in transgenic models compared to the human condition.
T11632	54162-54166	RB	denotes	Thus
T11633	54212-54214	VBZ	denotes	is
T11634	54166-54168	,	denotes	,
T11635	54168-54171	DT	denotes	the
T11636	54172-54176	NN	denotes	role
T11637	54177-54179	IN	denotes	of
T11638	54180-54189	NNS	denotes	microglia
T11639	54190-54192	IN	denotes	in
T11640	54193-54200	NN	denotes	amyloid
T11641	54201-54211	NN	denotes	metabolism
T11642	54215-54220	JJ	denotes	minor
T11643	54221-54223	IN	denotes	in
T11644	54224-54234	JJ	denotes	transgenic
T11645	54235-54241	NNS	denotes	models
T11646	54242-54250	VBN	denotes	compared
T11647	54251-54253	IN	denotes	to
T11648	54254-54257	DT	denotes	the
T11649	54264-54273	NN	denotes	condition
T11650	54258-54263	JJ	denotes	human
T11651	54273-54274	.	denotes	.
T11652	54274-54565	sentence	denotes	Somewhat paradoxically, several studies further demonstrate that treatment with anti-inflammatory drugs to reduce microglial activation actually lowers amyloid load in APP transgenic mice, suggesting a role for mouse microglia in the formation and maintenance of amyloid aggregates [41–43].
T11653	54275-54283	RB	denotes	Somewhat
T11654	54284-54297	RB	denotes	paradoxically
T11655	54323-54334	VBP	denotes	demonstrate
T11656	54297-54299	,	denotes	,
T11657	54299-54306	JJ	denotes	several
T11658	54307-54314	NNS	denotes	studies
T11659	54315-54322	RB	denotes	further
T11660	54335-54339	IN	denotes	that
T11661	54420-54426	VBZ	denotes	lowers
T11662	54340-54349	NN	denotes	treatment
T11663	54350-54354	IN	denotes	with
T11664	54355-54372	JJ	denotes	anti-inflammatory
T11665	54373-54378	NNS	denotes	drugs
T11666	54379-54381	TO	denotes	to
T11667	54382-54388	VB	denotes	reduce
T11668	54389-54399	JJ	denotes	microglial
T11669	54400-54410	NN	denotes	activation
T11670	54411-54419	RB	denotes	actually
T11671	54427-54434	NN	denotes	amyloid
T11672	54435-54439	NN	denotes	load
T11673	54440-54442	IN	denotes	in
T11674	54443-54446	NN	denotes	APP
T11675	54447-54457	JJ	denotes	transgenic
T11676	54458-54462	NNS	denotes	mice
T11677	54462-54464	,	denotes	,
T11678	54464-54474	VBG	denotes	suggesting
T11679	54475-54476	DT	denotes	a
T11680	54477-54481	NN	denotes	role
T11681	54482-54485	IN	denotes	for
T11682	54486-54491	NN	denotes	mouse
T11683	54492-54501	NNS	denotes	microglia
T11684	54502-54504	IN	denotes	in
T11685	54505-54508	DT	denotes	the
T11686	54509-54518	NN	denotes	formation
T11687	54519-54522	CC	denotes	and
T11688	54523-54534	NN	denotes	maintenance
T11689	54535-54537	IN	denotes	of
T11690	54538-54545	NN	denotes	amyloid
T11691	54546-54556	NNS	denotes	aggregates
T11692	54557-54558	-LRB-	denotes	[
T11693	54558-54560	CD	denotes	41
T11694	54560-54561	SYM	denotes	–
T11695	54561-54563	CD	denotes	43
T11696	54563-54564	-RRB-	denotes	]
T11697	54564-54565	.	denotes	.
T11698	54565-54701	sentence	denotes	However, this outcome may be alternatively explained by direct effects of many anti-inflammatory drugs on γ-secretase cleavage [44–47].
T11699	54566-54573	RB	denotes	However
T11700	54609-54618	VBN	denotes	explained
T11701	54573-54575	,	denotes	,
T11702	54575-54579	DT	denotes	this
T11703	54580-54587	NN	denotes	outcome
T11704	54588-54591	MD	denotes	may
T11705	54592-54594	VB	denotes	be
T11706	54595-54608	RB	denotes	alternatively
T11707	54619-54621	IN	denotes	by
T11708	54622-54628	JJ	denotes	direct
T11709	54629-54636	NNS	denotes	effects
T11710	54637-54639	IN	denotes	of
T11711	54640-54644	JJ	denotes	many
T11712	54663-54668	NNS	denotes	drugs
T11713	54645-54662	JJ	denotes	anti-inflammatory
T11714	54669-54671	IN	denotes	on
T11715	54672-54673	SYM	denotes	γ
T11716	54674-54683	NN	denotes	secretase
T11717	54673-54674	HYPH	denotes	-
T11718	54684-54692	NN	denotes	cleavage
T11719	54693-54694	-LRB-	denotes	[
T11720	54694-54696	CD	denotes	44
T11721	54696-54697	SYM	denotes	–
T11722	54697-54699	CD	denotes	47
T11723	54699-54700	-RRB-	denotes	]
T11724	54700-54701	.	denotes	.
T11725	54701-54992	sentence	denotes	Nonetheless, the role of microglia in both the human condition and the mouse models is poorly understood, and differences in microglial reactivity between the two could lead to significantly faster amyloid clearance in the brains of patients with AD than we observe in the tet-off APP mice.
T11726	54702-54713	RB	denotes	Nonetheless
T11727	54786-54788	VBZ	denotes	is
T11728	54713-54715	,	denotes	,
T11729	54715-54718	DT	denotes	the
T11730	54719-54723	NN	denotes	role
T11731	54724-54726	IN	denotes	of
T11732	54727-54736	NNS	denotes	microglia
T11733	54737-54739	IN	denotes	in
T11734	54740-54744	CC	denotes	both
T11735	54755-54764	NN	denotes	condition
T11736	54745-54748	DT	denotes	the
T11737	54749-54754	JJ	denotes	human
T11738	54765-54768	CC	denotes	and
T11739	54769-54772	DT	denotes	the
T11740	54779-54785	NNS	denotes	models
T11741	54773-54778	NN	denotes	mouse
T11742	54789-54795	RB	denotes	poorly
T11743	54796-54806	VBN	denotes	understood
T11744	54806-54808	,	denotes	,
T11745	54808-54811	CC	denotes	and
T11746	54812-54823	NNS	denotes	differences
T11747	54871-54875	VB	denotes	lead
T11748	54824-54826	IN	denotes	in
T11749	54827-54837	JJ	denotes	microglial
T11750	54838-54848	NN	denotes	reactivity
T11751	54849-54856	IN	denotes	between
T11752	54857-54860	DT	denotes	the
T11753	54861-54864	CD	denotes	two
T11754	54865-54870	MD	denotes	could
T11755	54876-54878	IN	denotes	to
T11756	54879-54892	RB	denotes	significantly
T11757	54893-54899	JJR	denotes	faster
T11758	54908-54917	NN	denotes	clearance
T11759	54900-54907	NN	denotes	amyloid
T11760	54918-54920	IN	denotes	in
T11761	54921-54924	DT	denotes	the
T11762	54925-54931	NNS	denotes	brains
T11763	54932-54934	IN	denotes	of
T11764	54935-54943	NNS	denotes	patients
T11765	54944-54948	IN	denotes	with
T11766	54949-54951	NN	denotes	AD
T11767	54952-54956	IN	denotes	than
T11768	54960-54967	VBP	denotes	observe
T11769	54957-54959	PRP	denotes	we
T11770	54968-54970	IN	denotes	in
T11771	54971-54974	DT	denotes	the
T11772	54987-54991	NNS	denotes	mice
T11773	54975-54978	NN	denotes	tet
T11774	54979-54982	NN	denotes	off
T11775	54978-54979	HYPH	denotes	-
T11776	54983-54986	NN	denotes	APP
T11777	54991-54992	.	denotes	.
T11778	54992-55191	sentence	denotes	Given the relatively minor role played by microglia in other mouse models of amyloidosis, we think it unlikely that these cells have influenced the rate of amyloid clearance in the tet-off APP mice.
T11779	54993-54998	VBN	denotes	Given
T11780	55086-55091	VBP	denotes	think
T11781	54999-55002	DT	denotes	the
T11782	55020-55024	NN	denotes	role
T11783	55003-55013	RB	denotes	relatively
T11784	55014-55019	JJ	denotes	minor
T11785	55025-55031	VBN	denotes	played
T11786	55032-55034	IN	denotes	by
T11787	55035-55044	NNS	denotes	microglia
T11788	55045-55047	IN	denotes	in
T11789	55048-55053	JJ	denotes	other
T11790	55060-55066	NNS	denotes	models
T11791	55054-55059	NN	denotes	mouse
T11792	55067-55069	IN	denotes	of
T11793	55070-55081	NN	denotes	amyloidosis
T11794	55081-55083	,	denotes	,
T11795	55083-55085	PRP	denotes	we
T11796	55092-55094	PRP	denotes	it
T11797	55095-55103	JJ	denotes	unlikely
T11798	55104-55108	IN	denotes	that
T11799	55126-55136	VBN	denotes	influenced
T11800	55109-55114	DT	denotes	these
T11801	55115-55120	NNS	denotes	cells
T11802	55121-55125	VBP	denotes	have
T11803	55137-55140	DT	denotes	the
T11804	55141-55145	NN	denotes	rate
T11805	55146-55148	IN	denotes	of
T11806	55149-55156	NN	denotes	amyloid
T11807	55157-55166	NN	denotes	clearance
T11808	55167-55169	IN	denotes	in
T11809	55170-55173	DT	denotes	the
T11810	55186-55190	NNS	denotes	mice
T11811	55174-55177	NN	denotes	tet
T11812	55178-55181	NN	denotes	off
T11813	55177-55178	HYPH	denotes	-
T11814	55182-55185	NN	denotes	APP
T11815	55190-55191	.	denotes	.
T11816	55191-55329	sentence	denotes	Even so, we considered the possibility that chronic dox treatment may have altered the activation state of microglia in our treated mice.
T11817	55192-55196	RB	denotes	Even
T11818	55197-55199	RB	denotes	so
T11819	55204-55214	VBD	denotes	considered
T11820	55199-55201	,	denotes	,
T11821	55201-55203	PRP	denotes	we
T11822	55215-55218	DT	denotes	the
T11823	55219-55230	NN	denotes	possibility
T11824	55231-55235	IN	denotes	that
T11825	55267-55274	VBN	denotes	altered
T11826	55236-55243	JJ	denotes	chronic
T11827	55248-55257	NN	denotes	treatment
T11828	55244-55247	NN	denotes	dox
T11829	55258-55261	MD	denotes	may
T11830	55262-55266	VB	denotes	have
T11831	55275-55278	DT	denotes	the
T11832	55290-55295	NN	denotes	state
T11833	55279-55289	NN	denotes	activation
T11834	55296-55298	IN	denotes	of
T11835	55299-55308	NNS	denotes	microglia
T11836	55309-55311	IN	denotes	in
T11837	55312-55315	PRP$	denotes	our
T11838	55324-55328	NNS	denotes	mice
T11839	55316-55323	VBN	denotes	treated
T11840	55328-55329	.	denotes	.
T11841	55329-55452	sentence	denotes	Dox is structurally similar to minocycline, a reported anti-inflammatory drug and inhibitor of microglial activation [48].
T11842	55330-55333	NN	denotes	Dox
T11843	55334-55336	VBZ	denotes	is
T11844	55337-55349	RB	denotes	structurally
T11845	55350-55357	JJ	denotes	similar
T11846	55358-55360	IN	denotes	to
T11847	55361-55372	NN	denotes	minocycline
T11848	55372-55374	,	denotes	,
T11849	55374-55375	DT	denotes	a
T11850	55403-55407	NN	denotes	drug
T11851	55376-55384	VBN	denotes	reported
T11852	55385-55402	JJ	denotes	anti-inflammatory
T11853	55408-55411	CC	denotes	and
T11854	55412-55421	NN	denotes	inhibitor
T11855	55422-55424	IN	denotes	of
T11856	55425-55435	JJ	denotes	microglial
T11857	55436-55446	NN	denotes	activation
T11858	55447-55448	-LRB-	denotes	[
T11859	55448-55450	CD	denotes	48
T11860	55450-55451	-RRB-	denotes	]
T11861	55451-55452	.	denotes	.
T11862	55452-55642	sentence	denotes	However, if dox does have anti-inflammatory activity, then, based on previous studies with other anti-inflammatories, we would have expected to find less amyloid in the dox-treated animals.
T11863	55453-55460	RB	denotes	However
T11864	55585-55593	VBN	denotes	expected
T11865	55460-55462	,	denotes	,
T11866	55462-55464	IN	denotes	if
T11867	55474-55478	VB	denotes	have
T11868	55465-55468	NN	denotes	dox
T11869	55469-55473	VBZ	denotes	does
T11870	55479-55496	JJ	denotes	anti-inflammatory
T11871	55497-55505	NN	denotes	activity
T11872	55505-55507	,	denotes	,
T11873	55507-55511	RB	denotes	then
T11874	55511-55513	,	denotes	,
T11875	55513-55518	VBN	denotes	based
T11876	55519-55521	IN	denotes	on
T11877	55522-55530	JJ	denotes	previous
T11878	55531-55538	NNS	denotes	studies
T11879	55539-55543	IN	denotes	with
T11880	55544-55549	JJ	denotes	other
T11881	55550-55569	NNS	denotes	anti-inflammatories
T11882	55569-55571	,	denotes	,
T11883	55571-55573	PRP	denotes	we
T11884	55574-55579	MD	denotes	would
T11885	55580-55584	VB	denotes	have
T11886	55594-55596	TO	denotes	to
T11887	55597-55601	VB	denotes	find
T11888	55602-55606	RBR	denotes	less
T11889	55607-55614	NN	denotes	amyloid
T11890	55615-55617	IN	denotes	in
T11891	55618-55621	DT	denotes	the
T11892	55634-55641	NNS	denotes	animals
T11893	55622-55625	NN	denotes	dox
T11894	55626-55633	VBN	denotes	treated
T11895	55625-55626	HYPH	denotes	-
T11896	55641-55642	.	denotes	.
T11897	55642-55674	sentence	denotes	Clearly, that was not the case.
T11898	55643-55650	RB	denotes	Clearly
T11899	55657-55660	VBD	denotes	was
T11900	55650-55652	,	denotes	,
T11901	55652-55656	DT	denotes	that
T11902	55661-55664	RB	denotes	not
T11903	55665-55668	DT	denotes	the
T11904	55669-55673	NN	denotes	case
T11905	55673-55674	.	denotes	.
T11906	55674-55968	sentence	denotes	While it is possible that dox acts in some other way to slow amyloid clearance, data from multiple studies demonstrate that microglial responses are normally weak in the mouse AD models [37–40], and thus it is doubtful that dox-mediated microglial inhibition affected the outcome of our study.
T11907	55675-55680	IN	denotes	While
T11908	55684-55686	VBZ	denotes	is
T11909	55681-55683	PRP	denotes	it
T11910	55782-55793	VBP	denotes	demonstrate
T11911	55687-55695	JJ	denotes	possible
T11912	55696-55700	IN	denotes	that
T11913	55705-55709	VBZ	denotes	acts
T11914	55701-55704	NN	denotes	dox
T11915	55710-55712	IN	denotes	in
T11916	55713-55717	DT	denotes	some
T11917	55724-55727	NN	denotes	way
T11918	55718-55723	JJ	denotes	other
T11919	55728-55730	TO	denotes	to
T11920	55731-55735	VB	denotes	slow
T11921	55736-55743	NN	denotes	amyloid
T11922	55744-55753	NN	denotes	clearance
T11923	55753-55755	,	denotes	,
T11924	55755-55759	NNS	denotes	data
T11925	55760-55764	IN	denotes	from
T11926	55765-55773	JJ	denotes	multiple
T11927	55774-55781	NNS	denotes	studies
T11928	55794-55798	IN	denotes	that
T11929	55820-55823	VBP	denotes	are
T11930	55799-55809	JJ	denotes	microglial
T11931	55810-55819	NNS	denotes	responses
T11932	55824-55832	RB	denotes	normally
T11933	55833-55837	JJ	denotes	weak
T11934	55838-55840	IN	denotes	in
T11935	55841-55844	DT	denotes	the
T11936	55854-55860	NNS	denotes	models
T11937	55845-55850	NN	denotes	mouse
T11938	55851-55853	NN	denotes	AD
T11939	55861-55862	-LRB-	denotes	[
T11940	55862-55864	CD	denotes	37
T11941	55864-55865	SYM	denotes	–
T11942	55865-55867	CD	denotes	40
T11943	55867-55868	-RRB-	denotes	]
T11944	55868-55870	,	denotes	,
T11945	55870-55873	CC	denotes	and
T11946	55874-55878	RB	denotes	thus
T11947	55882-55884	VBZ	denotes	is
T11948	55879-55881	PRP	denotes	it
T11949	55885-55893	JJ	denotes	doubtful
T11950	55894-55898	IN	denotes	that
T11951	55934-55942	VBD	denotes	affected
T11952	55899-55902	NN	denotes	dox
T11953	55903-55911	VBN	denotes	mediated
T11954	55902-55903	HYPH	denotes	-
T11955	55923-55933	NN	denotes	inhibition
T11956	55912-55922	JJ	denotes	microglial
T11957	55943-55946	DT	denotes	the
T11958	55947-55954	NN	denotes	outcome
T11959	55955-55957	IN	denotes	of
T11960	55958-55961	PRP$	denotes	our
T11961	55962-55967	NN	denotes	study
T11962	55967-55968	.	denotes	.
T11963	55968-56150	sentence	denotes	The persistence and stability of amyloid deposits in our system is unexpected given the speed with which Aβ aggregates are cleared in other mouse models of therapeutic intervention.
T11964	55969-55972	DT	denotes	The
T11965	55973-55984	NN	denotes	persistence
T11966	56033-56035	VBZ	denotes	is
T11967	55985-55988	CC	denotes	and
T11968	55989-55998	NN	denotes	stability
T11969	55999-56001	IN	denotes	of
T11970	56002-56009	NN	denotes	amyloid
T11971	56010-56018	NNS	denotes	deposits
T11972	56019-56021	IN	denotes	in
T11973	56022-56025	PRP$	denotes	our
T11974	56026-56032	NN	denotes	system
T11975	56036-56046	JJ	denotes	unexpected
T11976	56047-56052	VBN	denotes	given
T11977	56053-56056	DT	denotes	the
T11978	56057-56062	NN	denotes	speed
T11979	56063-56067	IN	denotes	with
T11980	56092-56099	VBN	denotes	cleared
T11981	56068-56073	WDT	denotes	which
T11982	56074-56076	NN	denotes	Aβ
T11983	56077-56087	NNS	denotes	aggregates
T11984	56088-56091	VBP	denotes	are
T11985	56100-56102	IN	denotes	in
T11986	56103-56108	JJ	denotes	other
T11987	56115-56121	NNS	denotes	models
T11988	56109-56114	NN	denotes	mouse
T11989	56122-56124	IN	denotes	of
T11990	56125-56136	JJ	denotes	therapeutic
T11991	56137-56149	NN	denotes	intervention
T11992	56149-56150	.	denotes	.
T11993	56150-56294	sentence	denotes	Anti-Aβ antibodies injected directly into the brain have been shown to eliminate amyloid deposits in as little as 1 wk after treatment [49–51].
T11994	56151-56158	JJ	denotes	Anti-Aβ
T11995	56159-56169	NNS	denotes	antibodies
T11996	56213-56218	VBN	denotes	shown
T11997	56170-56178	VBN	denotes	injected
T11998	56179-56187	RB	denotes	directly
T11999	56188-56192	IN	denotes	into
T12000	56193-56196	DT	denotes	the
T12001	56197-56202	NN	denotes	brain
T12002	56203-56207	VBP	denotes	have
T12003	56208-56212	VBN	denotes	been
T12004	56219-56221	TO	denotes	to
T12005	56222-56231	VB	denotes	eliminate
T12006	56232-56239	NN	denotes	amyloid
T12007	56240-56248	NNS	denotes	deposits
T12008	56249-56251	IN	denotes	in
T12009	56252-56254	RB	denotes	as
T12010	56265-56266	CD	denotes	1
T12011	56255-56261	JJ	denotes	little
T12012	56262-56264	IN	denotes	as
T12013	56267-56269	NN	denotes	wk
T12014	56270-56275	IN	denotes	after
T12015	56276-56285	NN	denotes	treatment
T12016	56286-56287	-LRB-	denotes	[
T12017	56287-56289	CD	denotes	49
T12018	56289-56290	SYM	denotes	–
T12019	56290-56292	CD	denotes	51
T12020	56292-56293	-RRB-	denotes	]
T12021	56293-56294	.	denotes	.
T12022	56294-56511	sentence	denotes	Peripheral antibody injection decreases amyloid load more broadly, and although it does not appear to act as quickly as local injection, can significantly reduce amyloid load within 2 mo of initial treatment [52,53].
T12023	56295-56305	JJ	denotes	Peripheral
T12024	56315-56324	NN	denotes	injection
T12025	56306-56314	NN	denotes	antibody
T12026	56325-56334	VBZ	denotes	decreases
T12027	56335-56342	NN	denotes	amyloid
T12028	56343-56347	NN	denotes	load
T12029	56348-56352	RBR	denotes	more
T12030	56353-56360	RB	denotes	broadly
T12031	56360-56362	,	denotes	,
T12032	56362-56365	CC	denotes	and
T12033	56366-56374	IN	denotes	although
T12034	56387-56393	VB	denotes	appear
T12035	56375-56377	PRP	denotes	it
T12036	56378-56382	VBZ	denotes	does
T12037	56383-56386	RB	denotes	not
T12038	56450-56456	VB	denotes	reduce
T12039	56394-56396	TO	denotes	to
T12040	56397-56400	VB	denotes	act
T12041	56401-56403	RB	denotes	as
T12042	56404-56411	RB	denotes	quickly
T12043	56412-56414	IN	denotes	as
T12044	56415-56420	JJ	denotes	local
T12045	56421-56430	NN	denotes	injection
T12046	56430-56432	,	denotes	,
T12047	56432-56435	MD	denotes	can
T12048	56436-56449	RB	denotes	significantly
T12049	56457-56464	NN	denotes	amyloid
T12050	56465-56469	NN	denotes	load
T12051	56470-56476	IN	denotes	within
T12052	56477-56478	CD	denotes	2
T12053	56479-56481	NN	denotes	mo
T12054	56482-56484	IN	denotes	of
T12055	56485-56492	JJ	denotes	initial
T12056	56493-56502	NN	denotes	treatment
T12057	56503-56504	-LRB-	denotes	[
T12058	56507-56509	CD	denotes	53
T12059	56504-56506	CD	denotes	52
T12060	56506-56507	,	denotes	,
T12061	56509-56510	-RRB-	denotes	]
T12062	56510-56511	.	denotes	.
T12063	56511-56680	sentence	denotes	More recently, an alternative approach has shown that lentiviral transfer of neprilysin can also reduce the number of aggregates in the area of the injection site [54].
T12064	56512-56516	RBR	denotes	More
T12065	56517-56525	RB	denotes	recently
T12066	56555-56560	VBN	denotes	shown
T12067	56525-56527	,	denotes	,
T12068	56527-56529	DT	denotes	an
T12069	56542-56550	NN	denotes	approach
T12070	56530-56541	JJ	denotes	alternative
T12071	56551-56554	VBZ	denotes	has
T12072	56561-56565	IN	denotes	that
T12073	56609-56615	VB	denotes	reduce
T12074	56566-56576	JJ	denotes	lentiviral
T12075	56577-56585	NN	denotes	transfer
T12076	56586-56588	IN	denotes	of
T12077	56589-56599	NN	denotes	neprilysin
T12078	56600-56603	MD	denotes	can
T12079	56604-56608	RB	denotes	also
T12080	56616-56619	DT	denotes	the
T12081	56620-56626	NN	denotes	number
T12082	56627-56629	IN	denotes	of
T12083	56630-56640	NNS	denotes	aggregates
T12084	56641-56643	IN	denotes	in
T12085	56644-56647	DT	denotes	the
T12086	56648-56652	NN	denotes	area
T12087	56653-56655	IN	denotes	of
T12088	56656-56659	DT	denotes	the
T12089	56670-56674	NN	denotes	site
T12090	56660-56669	NN	denotes	injection
T12091	56675-56676	-LRB-	denotes	[
T12092	56676-56678	CD	denotes	54
T12093	56678-56679	-RRB-	denotes	]
T12094	56679-56680	.	denotes	.
T12095	56680-56890	sentence	denotes	Careful study of the mechanism behind several of the antibody-mediated therapies has suggested that activated microglia play an important role in the removal of fibrillar plaques after immunization [50,52,55].
T12096	56681-56688	JJ	denotes	Careful
T12097	56689-56694	NN	denotes	study
T12098	56766-56775	VBN	denotes	suggested
T12099	56695-56697	IN	denotes	of
T12100	56698-56701	DT	denotes	the
T12101	56702-56711	NN	denotes	mechanism
T12102	56712-56718	IN	denotes	behind
T12103	56719-56726	JJ	denotes	several
T12104	56727-56729	IN	denotes	of
T12105	56730-56733	DT	denotes	the
T12106	56752-56761	NNS	denotes	therapies
T12107	56734-56742	NN	denotes	antibody
T12108	56743-56751	VBN	denotes	mediated
T12109	56742-56743	HYPH	denotes	-
T12110	56762-56765	VBZ	denotes	has
T12111	56776-56780	IN	denotes	that
T12112	56801-56805	VBP	denotes	play
T12113	56781-56790	VBN	denotes	activated
T12114	56791-56800	NNS	denotes	microglia
T12115	56806-56808	DT	denotes	an
T12116	56819-56823	NN	denotes	role
T12117	56809-56818	JJ	denotes	important
T12118	56824-56826	IN	denotes	in
T12119	56827-56830	DT	denotes	the
T12120	56831-56838	NN	denotes	removal
T12121	56839-56841	IN	denotes	of
T12122	56842-56851	JJ	denotes	fibrillar
T12123	56852-56859	NNS	denotes	plaques
T12124	56860-56865	IN	denotes	after
T12125	56866-56878	NN	denotes	immunization
T12126	56879-56880	-LRB-	denotes	[
T12127	56886-56888	CD	denotes	55
T12128	56880-56882	CD	denotes	50
T12129	56882-56883	,	denotes	,
T12130	56883-56885	CD	denotes	52
T12131	56885-56886	,	denotes	,
T12132	56888-56889	-RRB-	denotes	]
T12133	56889-56890	.	denotes	.
T12134	56890-57135	sentence	denotes	However, it has been noted that deletion of the Fc receptor (the primary receptor for microglial opsinization of antibody–antigen complexes) in APP transgenic mouse models has no impact on the effectiveness of antibody-mediated therapy [56,57].
T12135	56891-56898	RB	denotes	However
T12136	56912-56917	VBN	denotes	noted
T12137	56898-56900	,	denotes	,
T12138	56900-56902	PRP	denotes	it
T12139	56903-56906	VBZ	denotes	has
T12140	56907-56911	VBN	denotes	been
T12141	56918-56922	IN	denotes	that
T12142	57063-57066	VBZ	denotes	has
T12143	56923-56931	NN	denotes	deletion
T12144	56932-56934	IN	denotes	of
T12145	56935-56938	DT	denotes	the
T12146	56942-56950	NN	denotes	receptor
T12147	56939-56941	NN	denotes	Fc
T12148	56951-56952	-LRB-	denotes	(
T12149	56952-56955	DT	denotes	the
T12150	56964-56972	NN	denotes	receptor
T12151	56956-56963	JJ	denotes	primary
T12152	56973-56976	IN	denotes	for
T12153	56977-56987	JJ	denotes	microglial
T12154	56988-57000	NN	denotes	opsinization
T12155	57001-57003	IN	denotes	of
T12156	57004-57012	NN	denotes	antibody
T12157	57013-57020	NN	denotes	antigen
T12158	57012-57013	HYPH	denotes	–
T12159	57021-57030	NNS	denotes	complexes
T12160	57030-57031	-RRB-	denotes	)
T12161	57032-57034	IN	denotes	in
T12162	57035-57038	NN	denotes	APP
T12163	57039-57049	JJ	denotes	transgenic
T12164	57056-57062	NNS	denotes	models
T12165	57050-57055	NN	denotes	mouse
T12166	57067-57069	DT	denotes	no
T12167	57070-57076	NN	denotes	impact
T12168	57077-57079	IN	denotes	on
T12169	57080-57083	DT	denotes	the
T12170	57084-57097	NN	denotes	effectiveness
T12171	57098-57100	IN	denotes	of
T12172	57101-57109	NN	denotes	antibody
T12173	57110-57118	VBN	denotes	mediated
T12174	57109-57110	HYPH	denotes	-
T12175	57119-57126	NN	denotes	therapy
T12176	57127-57128	-LRB-	denotes	[
T12177	57131-57133	CD	denotes	57
T12178	57128-57130	CD	denotes	56
T12179	57130-57131	,	denotes	,
T12180	57133-57134	-RRB-	denotes	]
T12181	57134-57135	.	denotes	.
T12182	57135-57406	sentence	denotes	It is, nevertheless, possible that lack of microglia activation is the major difference between the slow clearance described here, where no perturbation of the immune system is expected, and the rapid clearance described in studies involving antibody or viral injection.
T12183	57136-57138	PRP	denotes	It
T12184	57139-57141	VBZ	denotes	is
T12185	57141-57143	,	denotes	,
T12186	57143-57155	RB	denotes	nevertheless
T12187	57155-57157	,	denotes	,
T12188	57157-57165	JJ	denotes	possible
T12189	57166-57170	IN	denotes	that
T12190	57200-57202	VBZ	denotes	is
T12191	57171-57175	NN	denotes	lack
T12192	57176-57178	IN	denotes	of
T12193	57179-57188	NNS	denotes	microglia
T12194	57189-57199	NN	denotes	activation
T12195	57203-57206	DT	denotes	the
T12196	57213-57223	NN	denotes	difference
T12197	57207-57212	JJ	denotes	major
T12198	57224-57231	IN	denotes	between
T12199	57232-57235	DT	denotes	the
T12200	57241-57250	NN	denotes	clearance
T12201	57236-57240	JJ	denotes	slow
T12202	57251-57260	VBN	denotes	described
T12203	57261-57265	RB	denotes	here
T12204	57265-57267	,	denotes	,
T12205	57267-57272	WRB	denotes	where
T12206	57313-57321	VBN	denotes	expected
T12207	57273-57275	DT	denotes	no
T12208	57276-57288	NN	denotes	perturbation
T12209	57289-57291	IN	denotes	of
T12210	57292-57295	DT	denotes	the
T12211	57303-57309	NN	denotes	system
T12212	57296-57302	JJ	denotes	immune
T12213	57310-57312	VBZ	denotes	is
T12214	57321-57323	,	denotes	,
T12215	57323-57326	CC	denotes	and
T12216	57327-57330	DT	denotes	the
T12217	57337-57346	NN	denotes	clearance
T12218	57331-57336	JJ	denotes	rapid
T12219	57347-57356	VBN	denotes	described
T12220	57357-57359	IN	denotes	in
T12221	57360-57367	NNS	denotes	studies
T12222	57368-57377	VBG	denotes	involving
T12223	57378-57386	NN	denotes	antibody
T12224	57396-57405	NN	denotes	injection
T12225	57387-57389	CC	denotes	or
T12226	57390-57395	JJ	denotes	viral
T12227	57405-57406	.	denotes	.
T12228	57406-57687	sentence	denotes	In isolation, mild activation of microglia by injection damage or opsinization may not be adequate to induce substantial phagocytosis, but when combined with an Aβ-lowering agent, such as neprilysin or Aβ-targeted antibodies, the two may work in concert to clear peptide deposits.
T12229	57407-57409	IN	denotes	In
T12230	57494-57496	VB	denotes	be
T12231	57410-57419	NN	denotes	isolation
T12232	57419-57421	,	denotes	,
T12233	57421-57425	JJ	denotes	mild
T12234	57426-57436	NN	denotes	activation
T12235	57437-57439	IN	denotes	of
T12236	57440-57449	NNS	denotes	microglia
T12237	57450-57452	IN	denotes	by
T12238	57453-57462	NN	denotes	injection
T12239	57463-57469	NN	denotes	damage
T12240	57470-57472	CC	denotes	or
T12241	57473-57485	NN	denotes	opsinization
T12242	57486-57489	MD	denotes	may
T12243	57490-57493	RB	denotes	not
T12244	57497-57505	JJ	denotes	adequate
T12245	57506-57508	TO	denotes	to
T12246	57509-57515	VB	denotes	induce
T12247	57516-57527	JJ	denotes	substantial
T12248	57528-57540	NN	denotes	phagocytosis
T12249	57540-57542	,	denotes	,
T12250	57542-57545	CC	denotes	but
T12251	57546-57550	WRB	denotes	when
T12252	57551-57559	VBN	denotes	combined
T12253	57645-57649	VB	denotes	work
T12254	57560-57564	IN	denotes	with
T12255	57565-57567	DT	denotes	an
T12256	57580-57585	NN	denotes	agent
T12257	57568-57570	NN	denotes	Aβ
T12258	57571-57579	VBG	denotes	lowering
T12259	57570-57571	HYPH	denotes	-
T12260	57585-57587	,	denotes	,
T12261	57587-57591	JJ	denotes	such
T12262	57592-57594	IN	denotes	as
T12263	57595-57605	NN	denotes	neprilysin
T12264	57612-57620	VBN	denotes	targeted
T12265	57606-57608	CC	denotes	or
T12266	57609-57611	NN	denotes	Aβ
T12267	57611-57612	HYPH	denotes	-
T12268	57621-57631	NNS	denotes	antibodies
T12269	57631-57633	,	denotes	,
T12270	57633-57636	DT	denotes	the
T12271	57637-57640	CD	denotes	two
T12272	57641-57644	MD	denotes	may
T12273	57650-57652	IN	denotes	in
T12274	57653-57660	NN	denotes	concert
T12275	57661-57663	TO	denotes	to
T12276	57664-57669	VB	denotes	clear
T12277	57670-57677	NN	denotes	peptide
T12278	57678-57686	NNS	denotes	deposits
T12279	57686-57687	.	denotes	.
T12280	57687-57918	sentence	denotes	Consistent with this hypothesis, strong activation of microglia through transgenic expression of TGFβ [58] or central injection of lipopolysaccharide [59,60] can by itself substantially reduce plaque burden in APP transgenic mice.
T12281	57688-57698	JJ	denotes	Consistent
T12282	57874-57880	VB	denotes	reduce
T12283	57699-57703	IN	denotes	with
T12284	57704-57708	DT	denotes	this
T12285	57709-57719	NN	denotes	hypothesis
T12286	57719-57721	,	denotes	,
T12287	57721-57727	JJ	denotes	strong
T12288	57728-57738	NN	denotes	activation
T12289	57739-57741	IN	denotes	of
T12290	57742-57751	NNS	denotes	microglia
T12291	57752-57759	IN	denotes	through
T12292	57760-57770	JJ	denotes	transgenic
T12293	57771-57781	NN	denotes	expression
T12294	57782-57784	IN	denotes	of
T12295	57785-57789	NN	denotes	TGFβ
T12296	57790-57791	-LRB-	denotes	[
T12297	57791-57793	CD	denotes	58
T12298	57793-57794	-RRB-	denotes	]
T12299	57795-57797	CC	denotes	or
T12300	57798-57805	JJ	denotes	central
T12301	57806-57815	NN	denotes	injection
T12302	57816-57818	IN	denotes	of
T12303	57819-57837	NN	denotes	lipopolysaccharide
T12304	57838-57839	-LRB-	denotes	[
T12305	57842-57844	CD	denotes	60
T12306	57839-57841	CD	denotes	59
T12307	57841-57842	,	denotes	,
T12308	57844-57845	-RRB-	denotes	]
T12309	57846-57849	MD	denotes	can
T12310	57850-57852	IN	denotes	by
T12311	57853-57859	PRP	denotes	itself
T12312	57860-57873	RB	denotes	substantially
T12313	57881-57887	NN	denotes	plaque
T12314	57888-57894	NN	denotes	burden
T12315	57895-57897	IN	denotes	in
T12316	57898-57901	NN	denotes	APP
T12317	57902-57912	JJ	denotes	transgenic
T12318	57913-57917	NNS	denotes	mice
T12319	57917-57918	.	denotes	.
T12320	57918-58172	sentence	denotes	But in the case of acute antibody- and/or injury-mediated activation, once the inflammation has passed, and the antibody and bound peptide have been cleared and degraded, the remaining Aβ quickly reaggregates and amyloid pathology is reestablished [49].
T12321	57919-57922	CC	denotes	But
T12322	58115-58127	VBZ	denotes	reaggregates
T12323	57923-57925	IN	denotes	in
T12324	57926-57929	DT	denotes	the
T12325	57930-57934	NN	denotes	case
T12326	57935-57937	IN	denotes	of
T12327	57938-57943	JJ	denotes	acute
T12328	57977-57987	NN	denotes	activation
T12329	57944-57952	NN	denotes	antibody
T12330	57968-57976	VBN	denotes	mediated
T12331	57952-57953	HYPH	denotes	-
T12332	57954-57957	CC	denotes	and
T12333	57957-57958	HYPH	denotes	/
T12334	57958-57960	CC	denotes	or
T12335	57961-57967	NN	denotes	injury
T12336	57967-57968	HYPH	denotes	-
T12337	57987-57989	,	denotes	,
T12338	57989-57993	IN	denotes	once
T12339	58015-58021	VBN	denotes	passed
T12340	57994-57997	DT	denotes	the
T12341	57998-58010	NN	denotes	inflammation
T12342	58011-58014	VBZ	denotes	has
T12343	58021-58023	,	denotes	,
T12344	58023-58026	CC	denotes	and
T12345	58027-58030	DT	denotes	the
T12346	58031-58039	NN	denotes	antibody
T12347	58068-58075	VBN	denotes	cleared
T12348	58040-58043	CC	denotes	and
T12349	58044-58049	VBN	denotes	bound
T12350	58050-58057	NN	denotes	peptide
T12351	58058-58062	VBP	denotes	have
T12352	58063-58067	VBN	denotes	been
T12353	58076-58079	CC	denotes	and
T12354	58080-58088	VBN	denotes	degraded
T12355	58088-58090	,	denotes	,
T12356	58090-58093	DT	denotes	the
T12357	58104-58106	NN	denotes	Aβ
T12358	58094-58103	VBG	denotes	remaining
T12359	58107-58114	RB	denotes	quickly
T12360	58128-58131	CC	denotes	and
T12361	58132-58139	NN	denotes	amyloid
T12362	58140-58149	NN	denotes	pathology
T12363	58153-58166	VBN	denotes	reestablished
T12364	58150-58152	VBZ	denotes	is
T12365	58167-58168	-LRB-	denotes	[
T12366	58168-58170	CD	denotes	49
T12367	58170-58171	-RRB-	denotes	]
T12368	58171-58172	.	denotes	.
T12369	58172-58350	sentence	denotes	This finding reinforces the notion that without continued stimulation, microglia in mouse models do not maintain the same level of sustained activation that may occur in humans.
T12370	58173-58177	DT	denotes	This
T12371	58178-58185	NN	denotes	finding
T12372	58186-58196	VBZ	denotes	reinforces
T12373	58197-58200	DT	denotes	the
T12374	58201-58207	NN	denotes	notion
T12375	58208-58212	IN	denotes	that
T12376	58277-58285	VB	denotes	maintain
T12377	58213-58220	IN	denotes	without
T12378	58221-58230	VBN	denotes	continued
T12379	58231-58242	NN	denotes	stimulation
T12380	58242-58244	,	denotes	,
T12381	58244-58253	NNS	denotes	microglia
T12382	58254-58256	IN	denotes	in
T12383	58257-58262	NN	denotes	mouse
T12384	58263-58269	NNS	denotes	models
T12385	58270-58272	VBP	denotes	do
T12386	58273-58276	RB	denotes	not
T12387	58286-58289	DT	denotes	the
T12388	58295-58300	NN	denotes	level
T12389	58290-58294	JJ	denotes	same
T12390	58301-58303	IN	denotes	of
T12391	58304-58313	VBN	denotes	sustained
T12392	58314-58324	NN	denotes	activation
T12393	58325-58329	WDT	denotes	that
T12394	58334-58339	VB	denotes	occur
T12395	58330-58333	MD	denotes	may
T12396	58340-58342	IN	denotes	in
T12397	58343-58349	NNS	denotes	humans
T12398	58349-58350	.	denotes	.
T12399	58350-58465	sentence	denotes	SantaCruz et al. recently published a study of mice that express P301L human tau via a similar vector system [61].
T12400	58351-58360	NNP	denotes	SantaCruz
T12401	58377-58386	VBD	denotes	published
T12402	58361-58363	FW	denotes	et
T12403	58364-58367	FW	denotes	al.
T12404	58368-58376	RB	denotes	recently
T12405	58387-58388	DT	denotes	a
T12406	58389-58394	NN	denotes	study
T12407	58395-58397	IN	denotes	of
T12408	58398-58402	NNS	denotes	mice
T12409	58403-58407	WDT	denotes	that
T12410	58408-58415	VBP	denotes	express
T12411	58416-58421	NN	denotes	P301L
T12412	58428-58431	NN	denotes	tau
T12413	58422-58427	JJ	denotes	human
T12414	58432-58435	IN	denotes	via
T12415	58436-58437	DT	denotes	a
T12416	58453-58459	NN	denotes	system
T12417	58438-58445	JJ	denotes	similar
T12418	58446-58452	NN	denotes	vector
T12419	58460-58461	-LRB-	denotes	[
T12420	58461-58463	CD	denotes	61
T12421	58463-58464	-RRB-	denotes	]
T12422	58464-58465	.	denotes	.
T12423	58465-58633	sentence	denotes	As in our tet-off APP mice, SantaCruz et al. found that tau neurofibrillary tangles, like amyloid plaques, are not cleared efficiently following transgene suppression.
T12424	58466-58468	IN	denotes	As
T12425	58511-58516	VBD	denotes	found
T12426	58469-58471	IN	denotes	in
T12427	58472-58475	PRP$	denotes	our
T12428	58488-58492	NNS	denotes	mice
T12429	58476-58479	NN	denotes	tet
T12430	58480-58483	NN	denotes	off
T12431	58479-58480	HYPH	denotes	-
T12432	58484-58487	NN	denotes	APP
T12433	58492-58494	,	denotes	,
T12434	58494-58503	NNP	denotes	SantaCruz
T12435	58504-58506	FW	denotes	et
T12436	58507-58510	FW	denotes	al.
T12437	58517-58521	IN	denotes	that
T12438	58581-58588	VBN	denotes	cleared
T12439	58522-58525	NN	denotes	tau
T12440	58542-58549	NNS	denotes	tangles
T12441	58526-58541	JJ	denotes	neurofibrillary
T12442	58549-58551	,	denotes	,
T12443	58551-58555	IN	denotes	like
T12444	58556-58563	NN	denotes	amyloid
T12445	58564-58571	NNS	denotes	plaques
T12446	58571-58573	,	denotes	,
T12447	58573-58576	VBP	denotes	are
T12448	58577-58580	RB	denotes	not
T12449	58589-58600	RB	denotes	efficiently
T12450	58601-58610	VBG	denotes	following
T12451	58611-58620	NN	denotes	transgene
T12452	58621-58632	NN	denotes	suppression
T12453	58632-58633	.	denotes	.
T12454	58633-58836	sentence	denotes	The lack of clearance in both models of AD pathology comes as a stark contrast to the rapid removal of protein aggregates found in similar tet-off mouse models of Huntington [32] and prion disease [62].
T12455	58634-58637	DT	denotes	The
T12456	58638-58642	NN	denotes	lack
T12457	58687-58692	VBZ	denotes	comes
T12458	58643-58645	IN	denotes	of
T12459	58646-58655	NN	denotes	clearance
T12460	58656-58658	IN	denotes	in
T12461	58659-58663	DT	denotes	both
T12462	58664-58670	NNS	denotes	models
T12463	58671-58673	IN	denotes	of
T12464	58674-58676	NN	denotes	AD
T12465	58677-58686	NN	denotes	pathology
T12466	58693-58695	IN	denotes	as
T12467	58696-58697	DT	denotes	a
T12468	58704-58712	NN	denotes	contrast
T12469	58698-58703	JJ	denotes	stark
T12470	58713-58715	IN	denotes	to
T12471	58716-58719	DT	denotes	the
T12472	58726-58733	NN	denotes	removal
T12473	58720-58725	JJ	denotes	rapid
T12474	58734-58736	IN	denotes	of
T12475	58737-58744	NN	denotes	protein
T12476	58745-58755	NNS	denotes	aggregates
T12477	58756-58761	VBN	denotes	found
T12478	58762-58764	IN	denotes	in
T12479	58765-58772	JJ	denotes	similar
T12480	58787-58793	NNS	denotes	models
T12481	58773-58776	NN	denotes	tet
T12482	58777-58780	NN	denotes	off
T12483	58776-58777	HYPH	denotes	-
T12484	58781-58786	NN	denotes	mouse
T12485	58794-58796	IN	denotes	of
T12486	58797-58807	NNP	denotes	Huntington
T12487	58823-58830	NN	denotes	disease
T12488	58808-58809	-LRB-	denotes	[
T12489	58809-58811	CD	denotes	32
T12490	58811-58812	-RRB-	denotes	]
T12491	58813-58816	CC	denotes	and
T12492	58817-58822	NN	denotes	prion
T12493	58831-58832	-LRB-	denotes	[
T12494	58832-58834	CD	denotes	62
T12495	58834-58835	-RRB-	denotes	]
T12496	58835-58836	.	denotes	.
T12497	58836-58998	sentence	denotes	In these cases, disrupting the input of new monomer to the system via dox-mediated transgene suppression led to relatively rapid clearance of protein aggregates.
T12498	58837-58839	IN	denotes	In
T12499	58942-58945	VBD	denotes	led
T12500	58840-58845	DT	denotes	these
T12501	58846-58851	NNS	denotes	cases
T12502	58851-58853	,	denotes	,
T12503	58853-58863	VBG	denotes	disrupting
T12504	58864-58867	DT	denotes	the
T12505	58868-58873	NN	denotes	input
T12506	58874-58876	IN	denotes	of
T12507	58877-58880	JJ	denotes	new
T12508	58881-58888	NN	denotes	monomer
T12509	58889-58891	IN	denotes	to
T12510	58892-58895	DT	denotes	the
T12511	58896-58902	NN	denotes	system
T12512	58903-58906	IN	denotes	via
T12513	58907-58910	NN	denotes	dox
T12514	58911-58919	VBN	denotes	mediated
T12515	58910-58911	HYPH	denotes	-
T12516	58930-58941	NN	denotes	suppression
T12517	58920-58929	NN	denotes	transgene
T12518	58946-58948	IN	denotes	to
T12519	58949-58959	RB	denotes	relatively
T12520	58960-58965	JJ	denotes	rapid
T12521	58966-58975	NN	denotes	clearance
T12522	58976-58978	IN	denotes	of
T12523	58979-58986	NN	denotes	protein
T12524	58987-58997	NNS	denotes	aggregates
T12525	58997-58998	.	denotes	.
T12526	58998-59153	sentence	denotes	By contrast, our study and that of SantaCruz et al. suggest that protein aggregates in AD may be more tenacious than in other neurodegenerative disorders.
T12527	58999-59001	IN	denotes	By
T12528	59051-59058	VBP	denotes	suggest
T12529	59002-59010	NN	denotes	contrast
T12530	59010-59012	,	denotes	,
T12531	59012-59015	PRP$	denotes	our
T12532	59016-59021	NN	denotes	study
T12533	59022-59025	CC	denotes	and
T12534	59026-59030	DT	denotes	that
T12535	59031-59033	IN	denotes	of
T12536	59034-59043	NNP	denotes	SantaCruz
T12537	59044-59046	FW	denotes	et
T12538	59047-59050	FW	denotes	al.
T12539	59059-59063	IN	denotes	that
T12540	59093-59095	VB	denotes	be
T12541	59064-59071	NN	denotes	protein
T12542	59072-59082	NNS	denotes	aggregates
T12543	59083-59085	IN	denotes	in
T12544	59086-59088	NN	denotes	AD
T12545	59089-59092	MD	denotes	may
T12546	59096-59100	RBR	denotes	more
T12547	59101-59110	JJ	denotes	tenacious
T12548	59111-59115	IN	denotes	than
T12549	59116-59118	IN	denotes	in
T12550	59119-59124	JJ	denotes	other
T12551	59143-59152	NNS	denotes	disorders
T12552	59125-59142	JJ	denotes	neurodegenerative
T12553	59152-59153	.	denotes	.
T12554	59153-59321	sentence	denotes	Perhaps once aggregated, Aβ and tau are either inherently more stable than other protein aggregates or more resistant to intra- and extracellular clearance mechanisms.
T12555	59154-59161	RB	denotes	Perhaps
T12556	59190-59193	VBP	denotes	are
T12557	59162-59166	IN	denotes	once
T12558	59167-59177	VBN	denotes	aggregated
T12559	59177-59179	,	denotes	,
T12560	59179-59181	NN	denotes	Aβ
T12561	59182-59185	CC	denotes	and
T12562	59186-59189	NN	denotes	tau
T12563	59194-59200	RB	denotes	either
T12564	59217-59223	JJ	denotes	stable
T12565	59201-59211	RB	denotes	inherently
T12566	59212-59216	RBR	denotes	more
T12567	59224-59228	IN	denotes	than
T12568	59229-59234	JJ	denotes	other
T12569	59243-59253	NNS	denotes	aggregates
T12570	59235-59242	NN	denotes	protein
T12571	59254-59256	CC	denotes	or
T12572	59257-59261	RBR	denotes	more
T12573	59262-59271	JJ	denotes	resistant
T12574	59272-59274	IN	denotes	to
T12575	59275-59280	AFX	denotes	intra
T12576	59286-59299	JJ	denotes	extracellular
T12577	59280-59281	HYPH	denotes	-
T12578	59282-59285	CC	denotes	and
T12579	59310-59320	NNS	denotes	mechanisms
T12580	59300-59309	NN	denotes	clearance
T12581	59320-59321	.	denotes	.
T12582	59321-59462	sentence	denotes	One question we were not able to address in this study is whether abrogating synthesis of new Aβ halts the progression of cognitive decline.
T12583	59322-59325	CD	denotes	One
T12584	59326-59334	NN	denotes	question
T12585	59377-59379	VBZ	denotes	is
T12586	59335-59337	PRP	denotes	we
T12587	59338-59342	VBD	denotes	were
T12588	59343-59346	RB	denotes	not
T12589	59347-59351	JJ	denotes	able
T12590	59352-59354	TO	denotes	to
T12591	59355-59362	VB	denotes	address
T12592	59363-59365	IN	denotes	in
T12593	59366-59370	DT	denotes	this
T12594	59371-59376	NN	denotes	study
T12595	59380-59387	IN	denotes	whether
T12596	59419-59424	VBZ	denotes	halts
T12597	59388-59398	VBG	denotes	abrogating
T12598	59399-59408	NN	denotes	synthesis
T12599	59409-59411	IN	denotes	of
T12600	59412-59415	JJ	denotes	new
T12601	59416-59418	NN	denotes	Aβ
T12602	59425-59428	DT	denotes	the
T12603	59429-59440	NN	denotes	progression
T12604	59441-59443	IN	denotes	of
T12605	59444-59453	JJ	denotes	cognitive
T12606	59454-59461	NN	denotes	decline
T12607	59461-59462	.	denotes	.
T12608	59462-59619	sentence	denotes	Studies from the tet-off tau mice suggest that protein clearance may in fact not be required for cognitive improvement following transgene suppression [61].
T12609	59463-59470	NNS	denotes	Studies
T12610	59497-59504	VBP	denotes	suggest
T12611	59471-59475	IN	denotes	from
T12612	59476-59479	DT	denotes	the
T12613	59492-59496	NNS	denotes	mice
T12614	59480-59483	NN	denotes	tet
T12615	59484-59487	NN	denotes	off
T12616	59483-59484	HYPH	denotes	-
T12617	59488-59491	NN	denotes	tau
T12618	59505-59509	IN	denotes	that
T12619	59547-59555	VBN	denotes	required
T12620	59510-59517	NN	denotes	protein
T12621	59518-59527	NN	denotes	clearance
T12622	59528-59531	MD	denotes	may
T12623	59532-59534	IN	denotes	in
T12624	59535-59539	NN	denotes	fact
T12625	59540-59543	RB	denotes	not
T12626	59544-59546	VB	denotes	be
T12627	59556-59559	IN	denotes	for
T12628	59560-59569	JJ	denotes	cognitive
T12629	59570-59581	NN	denotes	improvement
T12630	59582-59591	VBG	denotes	following
T12631	59592-59601	NN	denotes	transgene
T12632	59602-59613	NN	denotes	suppression
T12633	59614-59615	-LRB-	denotes	[
T12634	59615-59617	CD	denotes	61
T12635	59617-59618	-RRB-	denotes	]
T12636	59618-59619	.	denotes	.
T12637	59619-59815	sentence	denotes	At present, because of unexpected noncognitive behavioral abnormalities, it is not clear whether the tet-off APP mice can be used to address the same question in the context of amyloid pathology.
T12638	59620-59622	IN	denotes	At
T12639	59696-59698	VBZ	denotes	is
T12640	59623-59630	JJ	denotes	present
T12641	59630-59632	,	denotes	,
T12642	59632-59639	IN	denotes	because
T12643	59640-59642	IN	denotes	of
T12644	59643-59653	JJ	denotes	unexpected
T12645	59678-59691	NNS	denotes	abnormalities
T12646	59654-59666	JJ	denotes	noncognitive
T12647	59667-59677	JJ	denotes	behavioral
T12648	59691-59693	,	denotes	,
T12649	59693-59695	PRP	denotes	it
T12650	59699-59702	RB	denotes	not
T12651	59703-59708	JJ	denotes	clear
T12652	59709-59716	IN	denotes	whether
T12653	59745-59749	VBN	denotes	used
T12654	59717-59720	DT	denotes	the
T12655	59733-59737	NNS	denotes	mice
T12656	59721-59724	NN	denotes	tet
T12657	59725-59728	NN	denotes	off
T12658	59724-59725	HYPH	denotes	-
T12659	59729-59732	NN	denotes	APP
T12660	59738-59741	MD	denotes	can
T12661	59742-59744	VB	denotes	be
T12662	59750-59752	TO	denotes	to
T12663	59753-59760	VB	denotes	address
T12664	59761-59764	DT	denotes	the
T12665	59770-59778	NN	denotes	question
T12666	59765-59769	JJ	denotes	same
T12667	59779-59781	IN	denotes	in
T12668	59782-59785	DT	denotes	the
T12669	59786-59793	NN	denotes	context
T12670	59794-59796	IN	denotes	of
T12671	59797-59804	NN	denotes	amyloid
T12672	59805-59814	NN	denotes	pathology
T12673	59814-59815	.	denotes	.
T12674	59815-59967	sentence	denotes	Both lines of tTA/APP mice we studied here display extreme hyperactivity visible as cage circling and quantified by activity monitoring (see Figure 7).
T12675	59816-59820	DT	denotes	Both
T12676	59821-59826	NNS	denotes	lines
T12677	59859-59866	VBP	denotes	display
T12678	59827-59829	IN	denotes	of
T12679	59830-59833	NN	denotes	tTA
T12680	59834-59837	NN	denotes	APP
T12681	59833-59834	HYPH	denotes	/
T12682	59838-59842	NNS	denotes	mice
T12683	59843-59845	PRP	denotes	we
T12684	59846-59853	VBN	denotes	studied
T12685	59854-59858	RB	denotes	here
T12686	59867-59874	JJ	denotes	extreme
T12687	59875-59888	NN	denotes	hyperactivity
T12688	59889-59896	JJ	denotes	visible
T12689	59897-59899	IN	denotes	as
T12690	59900-59904	NN	denotes	cage
T12691	59905-59913	NN	denotes	circling
T12692	59914-59917	CC	denotes	and
T12693	59918-59928	VBN	denotes	quantified
T12694	59929-59931	IN	denotes	by
T12695	59932-59940	NN	denotes	activity
T12696	59941-59951	NN	denotes	monitoring
T12697	59952-59953	-LRB-	denotes	(
T12698	59953-59956	VB	denotes	see
T12699	59957-59963	NN	denotes	Figure
T12700	59964-59965	CD	denotes	7
T12701	59965-59966	-RRB-	denotes	)
T12702	59966-59967	.	denotes	.
T12703	59967-60111	sentence	denotes	Many of the double transgenic mice showed similar circular patterns of swimming near the edge of the tank when tested in the Morris water maze.
T12704	59968-59972	JJ	denotes	Many
T12705	60003-60009	VBD	denotes	showed
T12706	59973-59975	IN	denotes	of
T12707	59976-59979	DT	denotes	the
T12708	59998-60002	NNS	denotes	mice
T12709	59980-59986	JJ	denotes	double
T12710	59987-59997	JJ	denotes	transgenic
T12711	60010-60017	JJ	denotes	similar
T12712	60027-60035	NNS	denotes	patterns
T12713	60018-60026	JJ	denotes	circular
T12714	60036-60038	IN	denotes	of
T12715	60039-60047	NN	denotes	swimming
T12716	60048-60052	IN	denotes	near
T12717	60053-60056	DT	denotes	the
T12718	60057-60061	NN	denotes	edge
T12719	60062-60064	IN	denotes	of
T12720	60065-60068	DT	denotes	the
T12721	60069-60073	NN	denotes	tank
T12722	60074-60078	WRB	denotes	when
T12723	60079-60085	VBN	denotes	tested
T12724	60086-60088	IN	denotes	in
T12725	60089-60092	DT	denotes	the
T12726	60106-60110	NN	denotes	maze
T12727	60093-60099	NNP	denotes	Morris
T12728	60100-60105	NN	denotes	water
T12729	60110-60111	.	denotes	.
T12730	60111-60201	sentence	denotes	Expression of this phenotype makes standard tests of learning and memory uninterpretable.
T12731	60112-60122	NN	denotes	Expression
T12732	60141-60146	VBZ	denotes	makes
T12733	60123-60125	IN	denotes	of
T12734	60126-60130	DT	denotes	this
T12735	60131-60140	NN	denotes	phenotype
T12736	60147-60155	JJ	denotes	standard
T12737	60156-60161	NNS	denotes	tests
T12738	60185-60200	JJ	denotes	uninterpretable
T12739	60162-60164	IN	denotes	of
T12740	60165-60173	NN	denotes	learning
T12741	60174-60177	CC	denotes	and
T12742	60178-60184	NN	denotes	memory
T12743	60200-60201	.	denotes	.
T12744	60201-60334	sentence	denotes	Hyperactivity nonspecifically inhibits choice-driven changes in movement, the key element behind all cognitive behavioral paradigms.
T12745	60202-60215	NN	denotes	Hyperactivity
T12746	60232-60240	VBZ	denotes	inhibits
T12747	60216-60231	RB	denotes	nonspecifically
T12748	60241-60247	NN	denotes	choice
T12749	60248-60254	VBN	denotes	driven
T12750	60247-60248	HYPH	denotes	-
T12751	60255-60262	NNS	denotes	changes
T12752	60263-60265	IN	denotes	in
T12753	60266-60274	NN	denotes	movement
T12754	60274-60276	,	denotes	,
T12755	60276-60279	DT	denotes	the
T12756	60284-60291	NN	denotes	element
T12757	60280-60283	JJ	denotes	key
T12758	60292-60298	IN	denotes	behind
T12759	60299-60302	DT	denotes	all
T12760	60324-60333	NNS	denotes	paradigms
T12761	60303-60312	JJ	denotes	cognitive
T12762	60313-60323	JJ	denotes	behavioral
T12763	60333-60334	.	denotes	.
T12764	60334-60483	sentence	denotes	We are currently working to determine whether hyperactivity correlates with expression of the APPswe/ind holoprotein or its proteolytic derivatives.
T12765	60335-60337	PRP	denotes	We
T12766	60352-60359	VBG	denotes	working
T12767	60338-60341	VBP	denotes	are
T12768	60342-60351	RB	denotes	currently
T12769	60360-60362	TO	denotes	to
T12770	60363-60372	VB	denotes	determine
T12771	60373-60380	IN	denotes	whether
T12772	60395-60405	VBZ	denotes	correlates
T12773	60381-60394	NN	denotes	hyperactivity
T12774	60406-60410	IN	denotes	with
T12775	60411-60421	NN	denotes	expression
T12776	60422-60424	IN	denotes	of
T12777	60425-60428	DT	denotes	the
T12778	60440-60451	NN	denotes	holoprotein
T12779	60429-60435	NN	denotes	APPswe
T12780	60436-60439	NN	denotes	ind
T12781	60435-60436	HYPH	denotes	/
T12782	60452-60454	CC	denotes	or
T12783	60455-60458	PRP$	denotes	its
T12784	60471-60482	NNS	denotes	derivatives
T12785	60459-60470	JJ	denotes	proteolytic
T12786	60482-60483	.	denotes	.
T12787	60483-60639	sentence	denotes	Preliminary studies suggest that hyperactivity does not appear quickly when dox-reared mice are shifted to nonmedicated diets (J. L. J., unpublished data).
T12788	60484-60495	JJ	denotes	Preliminary
T12789	60496-60503	NNS	denotes	studies
T12790	60504-60511	VBP	denotes	suggest
T12791	60512-60516	IN	denotes	that
T12792	60540-60546	VB	denotes	appear
T12793	60517-60530	NN	denotes	hyperactivity
T12794	60531-60535	VBZ	denotes	does
T12795	60536-60539	RB	denotes	not
T12796	60547-60554	RB	denotes	quickly
T12797	60555-60559	WRB	denotes	when
T12798	60580-60587	VBN	denotes	shifted
T12799	60560-60563	NN	denotes	dox
T12800	60564-60570	VBN	denotes	reared
T12801	60563-60564	HYPH	denotes	-
T12802	60571-60575	NNS	denotes	mice
T12803	60576-60579	VBP	denotes	are
T12804	60588-60590	IN	denotes	to
T12805	60591-60603	JJ	denotes	nonmedicated
T12806	60604-60609	NNS	denotes	diets
T12807	60610-60611	-LRB-	denotes	(
T12808	60633-60637	NNS	denotes	data
T12809	60611-60613	NNP	denotes	J.
T12810	60617-60619	NNP	denotes	J.
T12811	60614-60616	NNP	denotes	L.
T12812	60619-60621	,	denotes	,
T12813	60621-60632	JJ	denotes	unpublished
T12814	60637-60638	-RRB-	denotes	)
T12815	60638-60639	.	denotes	.
T12816	60639-60805	sentence	denotes	These data may indicate that the neuroactive culprit is not immediately present after transgenic APP synthesis is initiated, but requires additional time to develop.
T12817	60640-60645	DT	denotes	These
T12818	60646-60650	NNS	denotes	data
T12819	60655-60663	VB	denotes	indicate
T12820	60651-60654	MD	denotes	may
T12821	60664-60668	IN	denotes	that
T12822	60693-60695	VBZ	denotes	is
T12823	60669-60672	DT	denotes	the
T12824	60685-60692	NN	denotes	culprit
T12825	60673-60684	JJ	denotes	neuroactive
T12826	60696-60699	RB	denotes	not
T12827	60700-60711	RB	denotes	immediately
T12828	60712-60719	JJ	denotes	present
T12829	60720-60725	IN	denotes	after
T12830	60754-60763	VBN	denotes	initiated
T12831	60726-60736	JJ	denotes	transgenic
T12832	60741-60750	NN	denotes	synthesis
T12833	60737-60740	NN	denotes	APP
T12834	60751-60753	VBZ	denotes	is
T12835	60763-60765	,	denotes	,
T12836	60765-60768	CC	denotes	but
T12837	60769-60777	VBZ	denotes	requires
T12838	60778-60788	JJ	denotes	additional
T12839	60789-60793	NN	denotes	time
T12840	60794-60796	TO	denotes	to
T12841	60797-60804	VB	denotes	develop
T12842	60804-60805	.	denotes	.
T12843	60805-60936	sentence	denotes	Alternatively, hyperactivity may be caused by neuronal alterations due to transgene expression during early postnatal development.
T12844	60806-60819	RB	denotes	Alternatively
T12845	60842-60848	VBN	denotes	caused
T12846	60819-60821	,	denotes	,
T12847	60821-60834	NN	denotes	hyperactivity
T12848	60835-60838	MD	denotes	may
T12849	60839-60841	VB	denotes	be
T12850	60849-60851	IN	denotes	by
T12851	60852-60860	JJ	denotes	neuronal
T12852	60861-60872	NNS	denotes	alterations
T12853	60873-60876	IN	denotes	due
T12854	60877-60879	IN	denotes	to
T12855	60880-60889	NN	denotes	transgene
T12856	60890-60900	NN	denotes	expression
T12857	60901-60907	IN	denotes	during
T12858	60908-60913	JJ	denotes	early
T12859	60914-60923	JJ	denotes	postnatal
T12860	60924-60935	NN	denotes	development
T12861	60935-60936	.	denotes	.
T12862	60936-61011	sentence	denotes	Further experiments are needed to distinguish between these possibilities.
T12863	60937-60944	JJ	denotes	Further
T12864	60945-60956	NNS	denotes	experiments
T12865	60961-60967	VBN	denotes	needed
T12866	60957-60960	VBP	denotes	are
T12867	60968-60970	TO	denotes	to
T12868	60971-60982	VB	denotes	distinguish
T12869	60983-60990	IN	denotes	between
T12870	60991-60996	DT	denotes	these
T12871	60997-61010	NNS	denotes	possibilities
T12872	61010-61011	.	denotes	.
T12873	61011-61171	sentence	denotes	In summary, we demonstrate that abrogating Aβ production halts the progression of pathologic changes in a transgenic mouse model of Alzheimer-type amyloidosis.
T12874	61012-61014	IN	denotes	In
T12875	61027-61038	VBP	denotes	demonstrate
T12876	61015-61022	NN	denotes	summary
T12877	61022-61024	,	denotes	,
T12878	61024-61026	PRP	denotes	we
T12879	61039-61043	IN	denotes	that
T12880	61069-61074	VBZ	denotes	halts
T12881	61044-61054	VBG	denotes	abrogating
T12882	61055-61057	NN	denotes	Aβ
T12883	61058-61068	NN	denotes	production
T12884	61075-61078	DT	denotes	the
T12885	61079-61090	NN	denotes	progression
T12886	61091-61093	IN	denotes	of
T12887	61094-61104	JJ	denotes	pathologic
T12888	61105-61112	NNS	denotes	changes
T12889	61113-61115	IN	denotes	in
T12890	61116-61117	DT	denotes	a
T12891	61135-61140	NN	denotes	model
T12892	61118-61128	JJ	denotes	transgenic
T12893	61129-61134	NN	denotes	mouse
T12894	61141-61143	IN	denotes	of
T12895	61144-61153	NNP	denotes	Alzheimer
T12896	61154-61158	NN	denotes	type
T12897	61153-61154	HYPH	denotes	-
T12898	61159-61170	NN	denotes	amyloidosis
T12899	61170-61171	.	denotes	.
T12900	61171-61306	sentence	denotes	However, despite dramatic reductions in Aβ synthesis, neuritic plaques are stable structures in vivo that do not quickly disaggregate.
T12901	61172-61179	RB	denotes	However
T12902	61243-61246	VBP	denotes	are
T12903	61179-61181	,	denotes	,
T12904	61181-61188	IN	denotes	despite
T12905	61189-61197	JJ	denotes	dramatic
T12906	61198-61208	NNS	denotes	reductions
T12907	61209-61211	IN	denotes	in
T12908	61212-61214	NN	denotes	Aβ
T12909	61215-61224	NN	denotes	synthesis
T12910	61224-61226	,	denotes	,
T12911	61226-61234	JJ	denotes	neuritic
T12912	61235-61242	NNS	denotes	plaques
T12913	61247-61253	JJ	denotes	stable
T12914	61254-61264	NNS	denotes	structures
T12915	61265-61267	FW	denotes	in
T12916	61268-61272	FW	denotes	vivo
T12917	61273-61277	WDT	denotes	that
T12918	61293-61305	VB	denotes	disaggregate
T12919	61278-61280	VBP	denotes	do
T12920	61281-61284	RB	denotes	not
T12921	61285-61292	RB	denotes	quickly
T12922	61305-61306	.	denotes	.
T12923	61306-61502	sentence	denotes	It is possible that a combination of therapies to limit Aβ production, increase Aβ degradation, and enhance phagocytosis of deposited amyloid may be required to reverse damage associated with AD.
T12924	61307-61309	PRP	denotes	It
T12925	61310-61312	VBZ	denotes	is
T12926	61313-61321	JJ	denotes	possible
T12927	61322-61326	IN	denotes	that
T12928	61456-61464	VBN	denotes	required
T12929	61327-61328	DT	denotes	a
T12930	61329-61340	NN	denotes	combination
T12931	61341-61343	IN	denotes	of
T12932	61344-61353	NNS	denotes	therapies
T12933	61354-61356	TO	denotes	to
T12934	61357-61362	VB	denotes	limit
T12935	61363-61365	NN	denotes	Aβ
T12936	61366-61376	NN	denotes	production
T12937	61376-61378	,	denotes	,
T12938	61378-61386	VB	denotes	increase
T12939	61387-61389	NN	denotes	Aβ
T12940	61390-61401	NN	denotes	degradation
T12941	61401-61403	,	denotes	,
T12942	61403-61406	CC	denotes	and
T12943	61407-61414	VBP	denotes	enhance
T12944	61415-61427	NN	denotes	phagocytosis
T12945	61428-61430	IN	denotes	of
T12946	61431-61440	VBN	denotes	deposited
T12947	61441-61448	NN	denotes	amyloid
T12948	61449-61452	MD	denotes	may
T12949	61453-61455	VB	denotes	be
T12950	61465-61467	TO	denotes	to
T12951	61468-61475	VB	denotes	reverse
T12952	61476-61482	NN	denotes	damage
T12953	61483-61493	VBN	denotes	associated
T12954	61494-61498	IN	denotes	with
T12955	61499-61501	NN	denotes	AD
T12956	61501-61502	.	denotes	.
T12957	61502-61684	sentence	denotes	However, if started early enough in the course of disease, secretase inhibitors alone could provide substantial benefit in slowing pathogenic processes linked to amyloid deposition.
T12958	61503-61510	RB	denotes	However
T12959	61595-61602	VB	denotes	provide
T12960	61510-61512	,	denotes	,
T12961	61512-61514	IN	denotes	if
T12962	61515-61522	VBN	denotes	started
T12963	61523-61528	RB	denotes	early
T12964	61529-61535	RB	denotes	enough
T12965	61536-61538	IN	denotes	in
T12966	61539-61542	DT	denotes	the
T12967	61543-61549	NN	denotes	course
T12968	61550-61552	IN	denotes	of
T12969	61553-61560	NN	denotes	disease
T12970	61560-61562	,	denotes	,
T12971	61562-61571	NN	denotes	secretase
T12972	61572-61582	NNS	denotes	inhibitors
T12973	61583-61588	RB	denotes	alone
T12974	61589-61594	MD	denotes	could
T12975	61603-61614	JJ	denotes	substantial
T12976	61615-61622	NN	denotes	benefit
T12977	61623-61625	IN	denotes	in
T12978	61626-61633	VBG	denotes	slowing
T12979	61634-61644	JJ	denotes	pathogenic
T12980	61645-61654	NNS	denotes	processes
T12981	61655-61661	VBN	denotes	linked
T12982	61662-61664	IN	denotes	to
T12983	61665-61672	NN	denotes	amyloid
T12984	61673-61683	NN	denotes	deposition
T12985	61683-61684	.	denotes	.
T12986	61684-61919	sentence	denotes	Even at later stages in the disease, the presence of substantial microglial activation in human AD [40] suggests that simply slowing the formation of new amyloid deposits may allow ongoing phagocytosis to diminish preexisting lesions.
T12987	61685-61689	RB	denotes	Even
T12988	61690-61692	IN	denotes	at
T12989	61789-61797	VBZ	denotes	suggests
T12990	61693-61698	JJR	denotes	later
T12991	61699-61705	NNS	denotes	stages
T12992	61706-61708	IN	denotes	in
T12993	61709-61712	DT	denotes	the
T12994	61713-61720	NN	denotes	disease
T12995	61720-61722	,	denotes	,
T12996	61722-61725	DT	denotes	the
T12997	61726-61734	NN	denotes	presence
T12998	61735-61737	IN	denotes	of
T12999	61738-61749	JJ	denotes	substantial
T13000	61761-61771	NN	denotes	activation
T13001	61750-61760	JJ	denotes	microglial
T13002	61772-61774	IN	denotes	in
T13003	61775-61780	JJ	denotes	human
T13004	61781-61783	NN	denotes	AD
T13005	61784-61785	-LRB-	denotes	[
T13006	61785-61787	CD	denotes	40
T13007	61787-61788	-RRB-	denotes	]
T13008	61798-61802	IN	denotes	that
T13009	61860-61865	VB	denotes	allow
T13010	61803-61809	RB	denotes	simply
T13011	61810-61817	VBG	denotes	slowing
T13012	61818-61821	DT	denotes	the
T13013	61822-61831	NN	denotes	formation
T13014	61832-61834	IN	denotes	of
T13015	61835-61838	JJ	denotes	new
T13016	61847-61855	NNS	denotes	deposits
T13017	61839-61846	NN	denotes	amyloid
T13018	61856-61859	MD	denotes	may
T13019	61866-61873	JJ	denotes	ongoing
T13020	61874-61886	NN	denotes	phagocytosis
T13021	61890-61898	VB	denotes	diminish
T13022	61887-61889	TO	denotes	to
T13023	61899-61910	JJ	denotes	preexisting
T13024	61911-61918	NNS	denotes	lesions
T13025	61918-61919	.	denotes	.
T13026	61919-62158	sentence	denotes	However, the development of safe and effective secretase inhibitors will ultimately be required to determine whether the human brain has the capacity to repair amyloid-associated damage of AD once the progression of pathology is arrested.
T13027	61920-61927	RB	denotes	However
T13028	62007-62015	VBN	denotes	required
T13029	61927-61929	,	denotes	,
T13030	61929-61932	DT	denotes	the
T13031	61933-61944	NN	denotes	development
T13032	61945-61947	IN	denotes	of
T13033	61948-61952	JJ	denotes	safe
T13034	61977-61987	NNS	denotes	inhibitors
T13035	61953-61956	CC	denotes	and
T13036	61957-61966	JJ	denotes	effective
T13037	61967-61976	NN	denotes	secretase
T13038	61988-61992	MD	denotes	will
T13039	61993-62003	RB	denotes	ultimately
T13040	62004-62006	VB	denotes	be
T13041	62016-62018	TO	denotes	to
T13042	62019-62028	VB	denotes	determine
T13043	62029-62036	IN	denotes	whether
T13044	62053-62056	VBZ	denotes	has
T13045	62037-62040	DT	denotes	the
T13046	62047-62052	NN	denotes	brain
T13047	62041-62046	JJ	denotes	human
T13048	62057-62060	DT	denotes	the
T13049	62061-62069	NN	denotes	capacity
T13050	62070-62072	TO	denotes	to
T13051	62073-62079	VB	denotes	repair
T13052	62080-62087	NN	denotes	amyloid
T13053	62088-62098	VBN	denotes	associated
T13054	62087-62088	HYPH	denotes	-
T13055	62099-62105	NN	denotes	damage
T13056	62106-62108	IN	denotes	of
T13057	62109-62111	NN	denotes	AD
T13058	62112-62116	IN	denotes	once
T13059	62149-62157	VBN	denotes	arrested
T13060	62117-62120	DT	denotes	the
T13061	62121-62132	NN	denotes	progression
T13062	62133-62135	IN	denotes	of
T13063	62136-62145	NN	denotes	pathology
T13064	62146-62148	VBZ	denotes	is
T13065	62157-62158	.	denotes	.
T9668	47227-47229	NN	denotes	7C
T9669	47220-47226	NN	denotes	Figure
T9670	47229-47230	-RRB-	denotes	)
T9671	47230-47231	.	denotes	.
T9672	47231-47416	sentence	denotes	Intriguingly, all of the dox-reared animals, both transgenic and wild-type, showed altered circadian rhythms with far less distinction between their day- and nighttime activity levels.
T9673	47232-47244	RB	denotes	Intriguingly
T9674	47308-47314	VBD	denotes	showed
T9675	47244-47246	,	denotes	,
T9676	47246-47249	DT	denotes	all
T9677	47250-47252	IN	denotes	of
T9678	47253-47256	DT	denotes	the
T9679	47268-47275	NNS	denotes	animals
T9680	47257-47260	NN	denotes	dox
T9681	47261-47267	VBN	denotes	reared
T9682	47260-47261	HYPH	denotes	-
T9683	47275-47277	,	denotes	,
T9684	47277-47281	CC	denotes	both
T9685	47282-47292	JJ	denotes	transgenic
T9686	47293-47296	CC	denotes	and
T136	0-10	JJ	denotes	Persistent
T9661	47178-47184	NNS	denotes	levels
T9662	47185-47192	JJ	denotes	similar
T9663	47193-47195	IN	denotes	to
T9664	47196-47199	DT	denotes	the
T9665	47210-47218	NNS	denotes	controls
T9666	47200-47209	JJ	denotes	untreated
T9667	47219-47220	-LRB-	denotes	(
R1427	T2158	T2159	amod	Pronuclear,Injection
R1428	T2160	T2159	punct	", ",Injection
R1429	T2161	T2159	conj	Screening,Injection
R1430	T2162	T2161	prep	of,Screening
R1431	T2163	T2162	pobj	Founders,of
R1432	T2164	T2161	punct	", ",Screening
R1433	T2165	T2161	cc	and,Screening
R1434	T2166	T2161	conj	Maintenance,Screening
R1435	T2167	T2166	prep	of,Maintenance
R1436	T2168	T2169	det	the,Lines
R1437	T2169	T2167	pobj	Lines,of
R1438	T2171	T2172	det	The,vector
R1439	T2172	T2182	nsubjpass	vector,linearized
R1440	T2173	T2174	compound	moPrP,ind
R1441	T2174	T2172	compound	ind,vector
R1442	T2175	T2174	punct	-,ind
R1443	T2176	T2174	compound	tetP,ind
R1444	T2177	T2174	punct	-,ind
R1445	T2178	T2174	compound	mo,ind
R1446	T2179	T2174	punct	/,ind
R1447	T2180	T2174	compound	huAPP695swe,ind
R1448	T2181	T2174	punct	/,ind
R1449	T2183	T2182	auxpass	was,linearized
R1450	T2184	T2182	cc	and,linearized
R1451	T2185	T2186	det	the,domain
R1452	T2186	T2188	nsubj	domain,excised
R1453	T2187	T2186	compound	pBluescript,domain
R1454	T2188	T2182	conj	excised,linearized
R1455	T2189	T2188	prep	by,excised
R1456	T2190	T2189	pobj	digestion,by
R1457	T2191	T2190	prep	with,digestion
R1458	T2192	T2191	pobj	NotI,with
R1459	T2193	T2188	punct	.,excised
R1460	T2195	T2196	det	The,vector
R1461	T2196	T2198	nsubjpass	vector,injected
R1462	T2197	T2196	amod	purified,vector
R1463	T2199	T2198	auxpass	was,injected
R1464	T2200	T2198	prep	into,injected
R1465	T2201	T2202	det	the,pronucleus
R1466	T2202	T2200	pobj	pronucleus,into
R1467	T2203	T2202	prep	of,pronucleus
R1468	T2204	T2205	amod	fertilized,eggs
R1469	T2205	T2203	pobj	eggs,of
R1470	T2206	T2205	prep	from,eggs
R1471	T2207	T2208	nmod	C57BL,C3HeJ
R1472	T2208	T2212	compound	C3HeJ,matings
R1473	T2209	T2208	punct	/,C3HeJ
R1474	T2210	T2208	nmod	6J,C3HeJ
R1475	T2211	T2208	punct	×,C3HeJ
R1476	T2212	T2206	pobj	matings,from
R1477	T2213	T2212	compound	F1,matings
R1478	T2214	T2198	punct	.,injected
R1479	T2216	T2217	compound	Founder,animals
R1480	T2217	T2218	nsubjpass	animals,screened
R1481	T2219	T2218	auxpass	were,screened
R1482	T2220	T2218	prep	for,screened
R1483	T2221	T2222	det	the,presence
R1484	T2222	T2220	pobj	presence,for
R1485	T2223	T2222	prep	of,presence
R1486	T2224	T2225	det	the,transgene
R1487	T2225	T2223	pobj	transgene,of
R1488	T2226	T2218	prep	by,screened
R1489	T2227	T2228	nummod	three,way
R1490	T2228	T2230	compound	way,PCR
R1491	T2229	T2228	punct	-,way
R1492	T2230	T2226	pobj	PCR,by
R1493	T2231	T2218	advcl	using,screened
R1494	T2232	T2233	det	the,primers
R1495	T2233	T2231	dobj	primers,using
R1496	T2234	T2233	nmod	S36,primers
R1497	T2235	T2234	cc	and,S36
R1498	T2236	T2237	compound	PrP,AS
R1500	T2238	T2237	punct	-,AS
R1501	T2239	T2237	compound	S,AS
R1502	T2240	T2237	punct	/,AS
R1503	T2241	T2237	compound	PrP,AS
R1504	T2242	T2237	punct	-,AS
R1505	T2243	T2233	acl	described,primers
R1506	T2244	T2243	advmod	below,described
R1507	T2245	T2218	punct	.,screened
R1508	T2247	T2248	npadvmod	Transgene,positive
R1509	T2248	T2250	amod	positive,founders
R1510	T2249	T2248	punct	-,positive
R1511	T2250	T2251	nsubjpass	founders,bred
R1512	T2252	T2251	auxpass	were,bred
R1513	T2253	T2251	prep	to,bred
R1514	T2254	T2253	pobj	animals,to
R1515	T2255	T2254	acl	expressing,animals
R1516	T2256	T2257	det	the,transactivator
R1517	T2257	T2255	dobj	transactivator,expressing
R1518	T2258	T2257	compound	tetracycline,transactivator
R1519	T2259	T2257	punct	(,transactivator
R1520	T2260	T2257	appos	tTA,transactivator
R1521	T2261	T2251	punct	),bred
R1522	T2262	T2251	prep	under,bred
R1523	T2263	T2262	pobj	control,under
R1524	T2264	T2263	prep	of,control
R1525	T2265	T2266	det	the,promoter
R1526	T2266	T2264	pobj	promoter,of
R1527	T2267	T2268	nmod	calcium,calmodulin
R1528	T2268	T2270	nmod	calmodulin,IIα
R1529	T2269	T2268	punct	-,calmodulin
R1530	T2270	T2266	nmod	IIα,promoter
R1531	T2271	T2270	nmod	kinase,IIα
R1532	T2272	T2266	punct	(,promoter
R1533	T2273	T2266	nmod	CaMKIIα,promoter
R1534	T2274	T2266	punct	),promoter
R1535	T2275	T2266	acl	obtained,promoter
R1536	T2276	T2275	prep	from,obtained
R1537	T2277	T2278	compound	Jackson,Laboratory
R1538	T2278	T2276	pobj	Laboratory,from
R1539	T2279	T2280	punct	[,16
R1540	T2280	T2278	parataxis	16,Laboratory
R1541	T2281	T2280	punct	],16
R1542	T2282	T2283	punct	(,Harbor
R1543	T2283	T2275	parataxis	Harbor,obtained
R1544	T2284	T2283	compound	Bar,Harbor
R1545	T2285	T2283	punct	", ",Harbor
R1546	T2286	T2283	npadvmod	Maine,Harbor
R1547	T2287	T2283	punct	", ",Harbor
R1548	T2288	T2289	compound	United,States
R1549	T2289	T2283	npadvmod	States,Harbor
R1550	T2290	T2283	punct	;,Harbor
R1551	T2291	T2292	dep	stock,1Mmay
R1552	T2292	T2283	dep	1Mmay,Harbor
R1553	T2293	T2291	punct	#,stock
R1554	T2294	T2291	nummod	3010,stock
R1555	T2295	T2292	punct	;,1Mmay
R1556	T2296	T2292	dep	B6,1Mmay
R1557	T2297	T2292	punct	;,1Mmay
R1558	T2298	T2292	nmod	CBA,1Mmay
R1559	T2299	T2292	punct	-,1Mmay
R1560	T2300	T2292	nmod	TgN,1Mmay
R1561	T2301	T2292	punct	[,1Mmay
R1562	T2302	T2292	nmod	Camk2a,1Mmay
R1563	T2303	T2292	punct	-,1Mmay
R1564	T2304	T2292	nmod	tTA,1Mmay
R1565	T2305	T2292	punct	],1Mmay
R1566	T2306	T2283	punct	),Harbor
R1567	T2307	T2251	punct	.,bred
R1568	T2309	T2310	det	The,colony
R1569	T2310	T2311	nsubjpass	colony,maintained
R1570	T2312	T2311	auxpass	was,maintained
R1571	T2313	T2311	advmod	thereafter,maintained
R1572	T2314	T2311	prep	by,maintained
R1573	T2315	T2314	pcomp	crossing,by
R1574	T2316	T2317	amod	single,offspring
R1576	T2318	T2317	amod	transgenic,offspring
R1577	T2319	T2317	nmod	tTA,offspring
R1578	T2320	T2319	cc	and,tTA
R1579	T2321	T2319	conj	APP,tTA
R1580	T2322	T2315	prep	for,crossing
R1581	T2323	T2322	pobj	each,for
R1582	T2324	T2323	prep	of,each
R1583	T2325	T2326	det	the,lines
R1584	T2326	T2324	pobj	lines,of
R1585	T2327	T2326	nummod	four,lines
R1586	T2328	T2326	compound	APP,lines
R1587	T2329	T2311	punct	.,maintained
R1588	T2331	T2332	det	All,mice
R1589	T2332	T2333	nsubjpass	mice,provided
R1590	T2334	T2333	auxpass	were,provided
R1591	T2335	T2336	amod	fresh,food
R1592	T2336	T2333	dobj	food,provided
R1593	T2337	T2336	cc	and,food
R1594	T2338	T2336	conj	water,food
R1595	T2339	T2340	advmod	ad,libitum
R1596	T2340	T2333	advmod	libitum,provided
R1597	T2341	T2333	punct	.,provided
R1598	T2343	T2344	compound	Animal,protocols
R1599	T2344	T2345	nsubjpass	protocols,approved
R1600	T2346	T2345	auxpass	were,approved
R1601	T2347	T2345	agent	by,approved
R1602	T2348	T2349	det	both,University
R1603	T2349	T2353	nmod	University,Committees
R1604	T2350	T2349	det	the,University
R1605	T2351	T2349	nmod	Johns,University
R1606	T2352	T2349	nmod	Hopkins,University
R1607	T2353	T2347	pobj	Committees,by
R1608	T2354	T2349	cc	and,University
R1609	T2355	T2356	det	the,Institute
R1610	T2356	T2349	conj	Institute,University
R1611	T2357	T2356	compound	California,Institute
R1612	T2358	T2356	prep	of,Institute
R1613	T2359	T2358	pobj	Technology,of
R1614	T2360	T2361	nmod	Institutional,Animal
R1615	T2361	T2353	nmod	Animal,Committees
R1616	T2362	T2361	appos	Care,Animal
R1617	T2363	T2362	cc	and,Care
R1618	T2364	T2362	conj	Use,Care
R1619	T2365	T2345	punct	.,approved
R1622	T2408	T2409	compound	Doxycycline,Administration
R1623	T2411	T2412	nsubjpass	Doxycycline,administered
R1624	T2413	T2411	punct	(,Doxycycline
R1625	T2414	T2411	appos	dox,Doxycycline
R1626	T2415	T2411	punct	),Doxycycline
R1627	T2416	T2412	auxpass	was,administered
R1628	T2417	T2412	prep	through,administered
R1629	T2418	T2419	advmod	commercially,available
R1630	T2419	T2420	amod	available,chow
R1631	T2420	T2417	pobj	chow,through
R1632	T2421	T2422	npadvmod	dox,containing
R1633	T2422	T2420	amod	containing,chow
R1634	T2423	T2422	punct	-,containing
R1635	T2424	T2425	punct	(,BioServ
R1636	T2425	T2412	parataxis	BioServ,administered
R1637	T2426	T2425	punct	", ",BioServ
R1638	T2427	T2425	npadvmod	Frenchtown,BioServ
R1639	T2428	T2425	punct	", ",BioServ
R1640	T2429	T2430	compound	New,Jersey
R1641	T2430	T2425	npadvmod	Jersey,BioServ
R1642	T2431	T2425	punct	", ",BioServ
R1643	T2432	T2433	compound	United,States
R1644	T2433	T2425	npadvmod	States,BioServ
R1645	T2434	T2425	punct	),BioServ
R1646	T2435	T2412	punct	.,administered
R1647	T2437	T2438	det	The,chow
R1648	T2438	T2439	nsubj	chow,contained
R1649	T2439	T2440	ccomp	contained,was
R1650	T2441	T2442	nummod	200,mg
R1651	T2442	T2439	dobj	mg,contained
R1652	T2443	T2444	punct	/,kg
R1653	T2444	T2442	prep	kg,mg
R1654	T2445	T2442	prep	of,mg
R1655	T2446	T2445	pobj	antibiotic,of
R1656	T2447	T2440	punct	;,was
R1657	T2448	T2440	prep	based,was
R1658	T2449	T2448	prep	on,based
R1659	T2450	T2451	amod	estimated,consumption
R1660	T2451	T2449	pobj	consumption,on
R1661	T2452	T2451	prep	of,consumption
R1662	T2453	T2454	nummod	5,g
R1663	T2454	T2452	pobj	g,of
R1664	T2455	T2454	prep	per,g
R1665	T2456	T2455	pobj	mouse,per
R1666	T2457	T2454	prep	per,g
R1667	T2458	T2457	pobj	day,per
R1668	T2459	T2440	punct	", ",was
R1669	T2460	T2461	det	the,dose
R1670	T2461	T2440	nsubj	dose,was
R1671	T2462	T2461	amod	expected,dose
R1672	T2463	T2461	prep	to,dose
R1673	T2464	T2465	det	each,animal
R1674	T2465	T2463	pobj	animal,to
R1675	T2466	T2467	nummod	1,mg
R1676	T2467	T2468	compound	mg,dox
R1677	T2468	T2440	attr	dox,was
R1678	T2469	T2468	prep	per,dox
R1679	T2470	T2469	pobj	day,per
R1680	T2471	T2440	punct	.,was
R1681	T2473	T2474	det	The,animal
R1682	T2474	T2479	nsubj	animal,received
R1683	T2475	T2474	amod	average,animal
R1684	T2476	T2477	nummod	25,g
R1685	T2477	T2474	compound	g,animal
R1686	T2478	T2477	punct	-,g
R1687	T2480	T2479	advmod	therefore,received
R1688	T2481	T2482	nummod	40,μg
R1689	T2482	T2479	dobj	μg,received
R1690	T2483	T2482	prep	of,μg
R1691	T2484	T2483	pobj	dox,of
R1692	T2485	T2482	prep	per,μg
R1693	T2486	T2487	compound	gram,weight
R1694	T2487	T2485	pobj	weight,per
R1695	T2488	T2487	compound	body,weight
R1696	T2489	T2482	prep	per,μg
R1697	T2490	T2489	pobj	day,per
R1698	T2491	T2479	punct	.,received
R1699	T2493	T2494	nsubjpass	Chow,changed
R1700	T2495	T2494	auxpass	was,changed
R1701	T2496	T2497	quantmod	1,2
R1702	T2497	T2499	nummod	2,times
R1703	T2498	T2497	punct	–,2
R1704	T2499	T2494	npadvmod	times,changed
R1705	T2500	T2499	prep	per,times
R1706	T2501	T2500	pobj	week,per
R1707	T2502	T2503	aux	to,prevent
R1708	T2503	T2494	advcl	prevent,changed
R1709	T2504	T2503	dobj	breakdown,prevent
R1710	T2505	T2504	prep	of,breakdown
R1711	T2506	T2507	det	the,antibiotic
R1712	T2507	T2505	pobj	antibiotic,of
R1713	T2508	T2494	punct	.,changed
R1718	T2637	T2638	nsubjpass	Offspring,genotyped
R1719	T2639	T2638	auxpass	were,genotyped
R1720	T2640	T2638	prep	for,genotyped
R1721	T2641	T2642	det	the,presence
R1722	T2642	T2640	pobj	presence,for
R1723	T2643	T2642	prep	of,presence
R1724	T2644	T2645	det	each,transgene
R1725	T2645	T2643	pobj	transgene,of
R1726	T2646	T2638	prep	by,genotyped
R1727	T2647	T2648	compound	PCR,amplification
R1728	T2648	T2646	pobj	amplification,by
R1729	T2649	T2648	prep	of,amplification
R1730	T2650	T2651	amod	genomic,DNA
R1731	T2651	T2649	pobj	DNA,of
R1732	T2652	T2651	acl	extracted,DNA
R1733	T2653	T2652	prep	from,extracted
R1734	T2654	T2655	det	a,biopsy
R1735	T2655	T2653	pobj	biopsy,from
R1736	T2656	T2657	nummod	5,mm
R1737	T2657	T2655	compound	mm,biopsy
R1738	T2658	T2657	punct	-,mm
R1739	T2659	T2655	compound	tail,biopsy
R1740	T2660	T2638	punct	.,genotyped
R1741	T2662	T2663	nsubjpass	Tails,heated
R1742	T2664	T2663	auxpass	were,heated
R1743	T2665	T2663	prep	to,heated
R1744	T2666	T2667	nummod	95,°C
R1745	T2667	T2665	pobj	°C,to
R1746	T2668	T2663	prep	for,heated
R1747	T2669	T2670	nummod	45,min
R1748	T2670	T2668	pobj	min,for
R1749	T2671	T2663	prep	in,heated
R1750	T2672	T2673	nummod	250,μl
R1751	T2673	T2671	pobj	μl,in
R1752	T2674	T2673	prep	of,μl
R1753	T2675	T2676	nummod	50,mM
R1754	T2676	T2677	compound	mM,NaOH
R1755	T2677	T2674	pobj	NaOH,of
R1756	T2678	T2663	punct	", ",heated
R1757	T2679	T2663	dep	vortexed,heated
R1758	T2680	T2663	punct	", ",heated
R1759	T2681	T2682	advmod	then,neutralized
R1760	T2682	T2663	dep	neutralized,heated
R1761	T2683	T2682	prep	with,neutralized
R1762	T2684	T2685	det	an,volume
R1763	T2685	T2683	pobj	volume,with
R1764	T2686	T2685	amod	equal,volume
R1765	T2687	T2685	prep	of,volume
R1766	T2688	T2689	nummod	0.5,M
R1767	T2689	T2690	compound	M,HCl
R1768	T2690	T2687	pobj	HCl,of
R1769	T2691	T2690	compound	Tris,HCl
R1770	T2692	T2690	punct	-,HCl
R1771	T2693	T2694	punct	(,pH
R1772	T2694	T2690	parataxis	pH,HCl
R1773	T2695	T2694	nummod	5.5,pH
R1774	T2696	T2694	punct	),pH
R1775	T2697	T2663	punct	.,heated
R1776	T2699	T2700	nsubjpass	Debris,sedimented
R1777	T2701	T2700	auxpass	was,sedimented
R1778	T2702	T2700	prep	by,sedimented
R1779	T2703	T2702	pobj	centrifugation,by
R1780	T2704	T2700	punct	", ",sedimented
R1781	T2705	T2700	cc	and,sedimented
R1782	T2706	T2707	nummod	3,μl
R1783	T2707	T2708	nsubjpass	μl,used
R1784	T2708	T2700	conj	used,sedimented
R1785	T2709	T2707	prep	of,μl
R1786	T2710	T2709	pobj	supernatant,of
R1787	T2711	T2708	auxpass	was,used
R1788	T2712	T2708	prep	for,used
R1789	T2713	T2712	pobj	amplification,for
R1790	T2714	T2708	punct	.,used
R1791	T2716	T2717	nsubjpass	Genotyping,performed
R1792	T2718	T2716	prep	for,Genotyping
R1793	T2719	T2720	nmod	APP,transgenes
R1794	T2720	T2718	pobj	transgenes,for
R1795	T2721	T2719	cc	and,APP
R1796	T2722	T2719	conj	tTA,APP
R1797	T2723	T2717	auxpass	was,performed
R1798	T2724	T2717	prep	in,performed
R1799	T2725	T2726	det	the,reaction
R1800	T2726	T2724	pobj	reaction,in
R1801	T2727	T2726	amod	same,reaction
R1802	T2728	T2726	compound	PCR,reaction
R1803	T2729	T2717	punct	", ",performed
R1804	T2730	T2717	advcl	using,performed
R1805	T2731	T2732	nummod	five,primers
R1806	T2732	T2730	dobj	primers,using
R1807	T2733	T2732	amod	separate,primers
R1808	T2734	T2717	punct	.,performed
R1809	T2736	T2737	nsubjpass	APP,amplified
R1810	T2738	T2737	auxpass	was,amplified
R1811	T2739	T2737	advcl	using,amplified
R1812	T2740	T2741	advmod	forward,S36
R1813	T2741	T2739	dobj	S36,using
R1814	T2742	T2741	compound	primer,S36
R1815	T2743	T2741	acl	located,S36
R1816	T2744	T2743	prep	in,located
R1817	T2745	T2746	det	the,end
R1818	T2746	T2744	pobj	end,in
R1819	T2747	T2746	nummod	3,end
R1820	T2748	T2747	punct	′,3
R1821	T2749	T2746	prep	of,end
R1822	T2750	T2751	det	the,cDNA
R1823	T2751	T2749	pobj	cDNA,of
R1824	T2752	T2751	compound	APP,cDNA
R1825	T2753	T2754	punct	(,ATG
R1826	T2754	T2751	parataxis	ATG,cDNA
R1827	T2755	T2754	compound	CCG,ATG
R1828	T2756	T2754	compound	AGA,ATG
R1829	T2757	T2754	compound	TCT,ATG
R1830	T2758	T2754	compound	CTG,ATG
R1831	T2759	T2754	compound	AAG,ATG
R1832	T2760	T2754	compound	TGA,ATG
R1833	T2761	T2754	compound	AGA,ATG
R1834	T2762	T2754	compound	TGG,ATG
R1835	T2763	T2754	punct	),ATG
R1836	T2764	T2741	cc	and,S36
R1837	T2765	T2766	amod	reverse,J
R1838	T2766	T2741	conj	J,S36
R1839	T2767	T2766	compound	primer,J
R1840	T2768	T2766	compound	PrP,J
R1841	T2769	T2766	punct	-,J
R1842	T2770	T2766	compound	AS,J
R1843	T2771	T2766	punct	-,J
R1844	T2772	T2766	acl	located,J
R1845	T2773	T2772	prep	in,located
R1846	T2774	T2775	det	the,UTR
R1847	T2775	T2773	pobj	UTR,in
R1848	T2776	T2775	nummod	3,UTR
R1849	T2777	T2776	punct	′,3
R1850	T2778	T2775	prep	of,UTR
R1851	T2779	T2780	det	the,vector
R1852	T2780	T2778	pobj	vector,of
R1853	T2781	T2782	punct	(,GC
R1854	T2782	T2775	parataxis	GC,UTR
R1855	T2783	T2782	compound	CCA,GC
R1856	T2784	T2782	compound	AGC,GC
R1857	T2785	T2782	compound	CTA,GC
R1858	T2786	T2782	compound	GAC,GC
R1859	T2787	T2782	compound	CAC,GC
R1860	T2788	T2782	compound	GAG,GC
R1861	T2789	T2782	compound	AAT,GC
R1862	T2790	T2782	punct	),GC
R1863	T2791	T2737	punct	.,amplified
R1864	T2793	T2794	det	The,transgene
R1865	T2794	T2796	nsubjpass	transgene,detected
R1866	T2795	T2794	compound	tTA,transgene
R1867	T2797	T2796	auxpass	was,detected
R1868	T2798	T2796	advcl	using,detected
R1869	T2799	T2800	det	a,set
R1870	T2800	T2798	dobj	set,using
R1871	T2801	T2800	compound	primer,set
R1872	T2802	T2803	dep	that,amplified
R1873	T2803	T2800	relcl	amplified,set
R1874	T2804	T2803	prep	across,amplified
R1875	T2805	T2806	poss	its,subdomains
R1876	T2806	T2804	pobj	subdomains,across
R1877	T2807	T2806	nummod	two,subdomains
R1878	T2808	T2803	prep	with,amplified
R1879	T2809	T2808	pobj	tet,with
R1880	T2810	T2809	advmod	forward,tet
R1881	T2811	T2809	acl	located,tet
R1882	T2812	T2811	prep	within,located
R1883	T2813	T2814	det	the,repressor
R1884	T2814	T2812	pobj	repressor,within
R1885	T2815	T2816	compound	Tn10,tetracycline
R1886	T2816	T2814	compound	tetracycline,repressor
R1887	T2817	T2818	punct	(,AG
R1888	T2818	T2814	parataxis	AG,repressor
R1889	T2819	T2818	compound	CGC,AG
R1890	T2820	T2818	compound	TGT,AG
R1891	T2821	T2818	compound	GGG,AG
R1892	T2822	T2818	compound	GCA,AG
R1893	T2823	T2818	compound	TTT,AG
R1894	T2824	T2818	compound	TAC,AG
R1895	T2825	T2818	compound	TTT,AG
R1896	T2826	T2818	punct	),AG
R1897	T2827	T2809	cc	and,tet
R1898	T2828	T2809	conj	tet,tet
R1899	T2829	T2828	amod	reverse,tet
R1900	T2830	T2828	prep	within,tet
R1901	T2831	T2832	det	the,VP16
R1902	T2832	T2830	pobj	VP16,within
R1903	T2833	T2832	compound	HSV1,VP16
R1904	T2834	T2835	punct	(,GTC
R1905	T2835	T2832	parataxis	GTC,VP16
R1906	T2836	T2835	compound	CAT,GTC
R1907	T2837	T2835	compound	GTC,GTC
R1908	T2838	T2835	compound	CAG,GTC
R1909	T2839	T2835	compound	ATC,GTC
R1910	T2840	T2835	compound	GAA,GTC
R1911	T2841	T2835	compound	ATC,GTC
R1912	T2842	T2835	punct	),GTC
R1913	T2843	T2796	punct	.,detected
R1914	T2845	T2846	det	All,reactions
R1915	T2846	T2847	nsubj	reactions,amplified
R1916	T2848	T2847	punct	", ",amplified
R1917	T2849	T2850	mark	whether,positive
R1918	T2850	T2847	advcl	positive,amplified
R1919	T2851	T2850	npadvmod	transgene,positive
R1920	T2852	T2850	punct	-,positive
R1921	T2853	T2850	cc	or,positive
R1922	T2854	T2850	conj	not,positive
R1923	T2855	T2847	punct	", ",amplified
R1924	T2856	T2857	det	a,segment
R1925	T2857	T2847	dobj	segment,amplified
R1926	T2858	T2857	prep	of,segment
R1927	T2859	T2860	det	the,gene
R1928	T2860	T2858	pobj	gene,of
R1929	T2861	T2860	amod	endogenous,gene
R1930	T2862	T2860	compound	prion,gene
R1931	T2863	T2860	compound	protein,gene
R1932	T2864	T2847	prep	as,amplified
R1933	T2865	T2866	det	a,control
R1934	T2866	T2864	pobj	control,as
R1935	T2867	T2866	prep	for,control
R1936	T2868	T2869	compound	DNA,quality
R1937	T2869	T2867	pobj	quality,for
R1938	T2870	T2847	advcl	using,amplified
R1939	T2871	T2872	det	a,primer
R1940	T2872	T2870	dobj	primer,using
R1941	T2873	T2872	amod	forward,primer
R1942	T2874	T2872	punct	", ",primer
R1943	T2875	T2876	compound	PrP,J
R1944	T2876	T2872	appos	J,primer
R1945	T2877	T2876	punct	-,J
R1946	T2878	T2876	compound	S,J
R1947	T2879	T2876	punct	-,J
R1948	T2880	T2872	punct	", ",primer
R1949	T2881	T2872	amod	specific,primer
R1950	T2882	T2881	prep	to,specific
R1951	T2883	T2884	det	the,frame
R1952	T2884	T2882	pobj	frame,to
R1953	T2885	T2884	nmod	mouse,frame
R1954	T2886	T2884	nmod	PrP,frame
R1955	T2887	T2888	amod	open,reading
R1956	T2888	T2884	compound	reading,frame
R1957	T2889	T2890	punct	(,GG
R1958	T2890	T2884	parataxis	GG,frame
R1959	T2891	T2890	compound	GGG,GG
R1960	T2892	T2890	compound	ACT,GG
R1961	T2893	T2890	compound	ATG,GG
R1962	T2894	T2890	compound	TGG,GG
R1963	T2895	T2890	compound	ACT,GG
R1964	T2896	T2890	compound	GAT,GG
R1965	T2897	T2890	compound	GTC,GG
R1966	T2898	T2890	punct	),GG
R1967	T2899	T2872	cc	and,primer
R1968	T2900	T2901	det	a,primer
R1969	T2901	T2872	conj	primer,primer
R1970	T2902	T2901	amod	reverse,primer
R1971	T2903	T2901	punct	", ",primer
R1972	T2904	T2905	compound	PrP,J
R1973	T2905	T2901	appos	J,primer
R1974	T2906	T2905	punct	-,J
R1975	T2907	T2905	compound	AS,J
R1976	T2908	T2905	punct	-,J
R1977	T2909	T2901	punct	", ",primer
R1978	T2910	T2901	acl	shared,primer
R1979	T2911	T2910	agent	by,shared
R1980	T2912	T2913	det	the,UTR
R1981	T2913	T2911	pobj	UTR,by
R1982	T2914	T2913	nummod	3,UTR
R1983	T2915	T2914	punct	′,3
R1984	T2916	T2913	prep	of,UTR
R1985	T2917	T2918	det	the,gene
R1986	T2918	T2916	pobj	gene,of
R1987	T2919	T2918	amod	endogenous,gene
R1988	T2920	T2918	compound	PrP,gene
R1989	T2921	T2918	cc	and,gene
R1990	T2922	T2923	det	the,vector
R1991	T2923	T2918	conj	vector,gene
R1992	T2924	T2923	compound	transgene,vector
R1993	T2925	T2847	punct	.,amplified
R1994	T2927	T2928	compound	Amplification,reactions
R1995	T2928	T2929	nsubjpass	reactions,run
R1996	T2930	T2929	auxpass	were,run
R1997	T2931	T2929	prep	for,run
R1998	T2932	T2933	nummod	37,cycles
R1999	T2933	T2931	pobj	cycles,for
R2000	T2934	T2929	prep	at,run
R2001	T2935	T2936	nummod	94,°C
R2002	T2936	T2934	pobj	°C,at
R2003	T2937	T2929	prep	for,run
R2004	T2938	T2939	nummod	30,s
R2005	T2939	T2937	pobj	s,for
R2006	T2940	T2929	punct	", ",run
R2007	T2941	T2942	nummod	64,°C
R2008	T2942	T2929	conj	°C,run
R2009	T2943	T2942	prep	for,°C
R2010	T2944	T2945	nummod	1,min
R2011	T2945	T2943	pobj	min,for
R2012	T2946	T2942	punct	", ",°C
R2013	T2947	T2942	cc	and,°C
R2014	T2948	T2949	nummod	72,°C
R2015	T2949	T2942	conj	°C,°C
R2016	T2950	T2949	prep	for,°C
R2017	T2951	T2952	nummod	1,min
R2018	T2952	T2950	pobj	min,for
R2019	T2953	T2929	punct	.,run
R2020	T2955	T2956	det	All,samples
R2021	T2956	T2957	nsubj	samples,gave
R2022	T2958	T2956	punct	", ",samples
R2023	T2959	T2956	amod	transgenic,samples
R2024	T2960	T2959	cc	and,transgenic
R2025	T2961	T2962	amod	wild,type
R2026	T2962	T2959	conj	type,transgenic
R2027	T2963	T2962	punct	-,type
R2028	T2964	T2957	punct	", ",gave
R2029	T2965	T2966	det	a,product
R2030	T2966	T2957	dobj	product,gave
R2031	T2967	T2968	nummod	750,bp
R2032	T2968	T2966	compound	bp,product
R2033	T2969	T2968	punct	-,bp
R2034	T2970	T2966	prep	from,product
R2035	T2971	T2972	det	the,gene
R2036	T2972	T2970	pobj	gene,from
R2037	T2973	T2972	amod	endogenous,gene
R2038	T2974	T2972	compound	PrP,gene
R2039	T2975	T2957	punct	.,gave
R2040	T2977	T2978	det	The,transgene
R2041	T2978	T2980	nsubj	transgene,yielded
R2042	T2979	T2978	compound	APP,transgene
R2043	T2980	T2981	ccomp	yielded,fell
R2044	T2982	T2983	det	an,band
R2045	T2983	T2980	dobj	band,yielded
R2046	T2984	T2983	amod	additional,band
R2047	T2985	T2983	prep	at,band
R2048	T2986	T2987	nummod	400,bp
R2049	T2987	T2985	pobj	bp,at
R2050	T2988	T2981	punct	;,fell
R2051	T2989	T2990	det	the,product
R2052	T2990	T2981	nsubj	product,fell
R2053	T2991	T2990	compound	tTA,product
R2054	T2992	T2993	advmod	in,between
R2055	T2993	T2981	advmod	between,fell
R2056	T2994	T2981	prep	at,fell
R2057	T2995	T2996	nummod	480,bp
R2058	T2996	T2994	pobj	bp,at
R2059	T2997	T2981	punct	.,fell
R2060	T3141	T3142	compound	Immunoblotting,Quantitation
R2061	T3143	T3142	punct	/,Quantitation
R2062	T3145	T3146	amod	Frozen,tissue
R2063	T3146	T3151	nsubjpass	tissue,homogenized
R2064	T3147	T3146	amod	cortical,tissue
R2065	T3148	T3147	cc	or,cortical
R2066	T3149	T3150	amod	whole,forebrain
R2067	T3150	T3147	conj	forebrain,cortical
R2068	T3152	T3151	auxpass	was,homogenized
R2069	T3153	T3151	prep	by,homogenized
R2070	T3154	T3153	pobj	sonication,by
R2071	T3155	T3151	prep	in,homogenized
R2072	T3156	T3157	nummod	five,volumes
R2073	T3157	T3155	pobj	volumes,in
R2074	T3158	T3157	prep	of,volumes
R2075	T3159	T3160	npadvmod	phosphate,buffered
R2076	T3160	T3162	amod	buffered,saline
R2077	T3161	T3160	punct	-,buffered
R2078	T3162	T3158	pobj	saline,of
R2079	T3163	T3162	punct	(,saline
R2080	T3164	T3162	appos	PBS,saline
R2081	T3165	T3151	punct	),homogenized
R2082	T3166	T3151	prep	with,homogenized
R2083	T3167	T3168	nummod	5,mM
R2084	T3168	T3169	nmod	mM,inhibitors
R2085	T3169	T3166	pobj	inhibitors,with
R2086	T3170	T3169	nmod	EDTA,inhibitors
R2087	T3171	T3170	cc	and,EDTA
R2088	T3172	T3170	conj	protease,EDTA
R2089	T3173	T3174	punct	(,cocktail
R2090	T3174	T3169	parataxis	cocktail,inhibitors
R2091	T3175	T3174	amod	Mammalian,cocktail
R2092	T3176	T3174	compound	cell,cocktail
R2093	T3177	T3174	punct	", ",cocktail
R2094	T3178	T3174	npadvmod	Sigma,cocktail
R2095	T3179	T3174	punct	", ",cocktail
R2096	T3180	T3181	compound	St.,Louis
R2097	T3181	T3174	npadvmod	Louis,cocktail
R2098	T3182	T3174	punct	", ",cocktail
R2099	T3183	T3174	npadvmod	Missouri,cocktail
R2100	T3184	T3174	punct	", ",cocktail
R2101	T3185	T3186	compound	United,States
R2102	T3186	T3174	npadvmod	States,cocktail
R2103	T3187	T3174	punct	),cocktail
R2104	T3188	T3151	punct	", ",homogenized
R2105	T3189	T3151	advcl	using,homogenized
R2106	T3190	T3191	det	a,sonicator
R2107	T3191	T3189	dobj	sonicator,using
R2108	T3192	T3191	compound	probe,sonicator
R2109	T3193	T3191	acl	set,sonicator
R2110	T3194	T3193	prep	to,set
R2111	T3195	T3196	nummod	50,%
R2112	T3196	T3197	compound	%,output
R2113	T3197	T3194	pobj	output,to
R2114	T3198	T3199	punct	(,TEKMAR
R2115	T3199	T3191	parataxis	TEKMAR,sonicator
R2116	T3200	T3199	punct	", ",TEKMAR
R2117	T3201	T3199	npadvmod	Cincinnati,TEKMAR
R2118	T3202	T3199	punct	", ",TEKMAR
R2119	T3203	T3199	npadvmod	Ohio,TEKMAR
R2120	T3204	T3199	punct	", ",TEKMAR
R2121	T3205	T3206	compound	United,States
R2122	T3206	T3199	npadvmod	States,TEKMAR
R2123	T3207	T3199	punct	),TEKMAR
R2124	T3208	T3151	punct	.,homogenized
R2125	T3210	T3211	prep	After,centrifuged
R2126	T3212	T3210	pobj	dilution,After
R2127	T3213	T3212	prep	with,dilution
R2128	T3214	T3215	det	an,volume
R2129	T3215	T3213	pobj	volume,with
R2130	T3216	T3215	amod	equal,volume
R2131	T3217	T3215	prep	of,volume
R2132	T3218	T3219	compound	PBS,protease
R2133	T3219	T3223	compound	protease,inhibitor
R2134	T3220	T3219	punct	/,protease
R2135	T3221	T3219	compound	EDTA,protease
R2136	T3222	T3219	punct	/,protease
R2137	T3223	T3217	pobj	inhibitor,of
R2138	T3224	T3211	punct	", ",centrifuged
R2139	T3225	T3226	det	the,samples
R2140	T3226	T3211	nsubjpass	samples,centrifuged
R2141	T3227	T3211	auxpass	were,centrifuged
R2142	T3228	T3211	advmod	briefly,centrifuged
R2143	T3229	T3211	cc	and,centrifuged
R2144	T3230	T3231	det	the,supernatant
R2145	T3231	T3232	nsubj	supernatant,used
R2146	T3232	T3211	conj	used,centrifuged
R2147	T3233	T3232	prep	for,used
R2148	T3234	T3233	pobj	analysis,for
R2149	T3235	T3211	punct	.,centrifuged
R2150	T3237	T3238	nummod	Fifty,micrograms
R2151	T3238	T3239	nmod	micrograms,6E10
R2152	T3239	T3241	nsubjpass	6E10,loaded
R2153	T3240	T3239	punct	(,6E10
R2154	T3242	T3239	cc	and,6E10
R2155	T3243	T3239	conj	CT15,6E10
R2156	T3244	T3243	punct	),CT15
R2157	T3245	T3239	cc	or,6E10
R2158	T3246	T3247	nummod	5,μg
R2159	T3247	T3248	nmod	μg,22C11
R2160	T3248	T3239	conj	22C11,6E10
R2161	T3249	T3248	punct	(,22C11
R2162	T3250	T3248	punct	),22C11
R2163	T3251	T3239	prep	of,6E10
R2164	T3252	T3253	compound	brain,homogenate
R2165	T3253	T3251	pobj	homogenate,of
R2166	T3254	T3241	auxpass	was,loaded
R2167	T3255	T3241	prep	per,loaded
R2168	T3256	T3255	pobj	lane,per
R2169	T3257	T3241	prep	onto,loaded
R2170	T3258	T3259	nummod	7.5,%
R2171	T3259	T3260	nmod	%,PAGE
R2172	T3260	T3277	compound	PAGE,gels
R2173	T3261	T3259	punct	", ",%
R2174	T3262	T3263	nummod	10,%
R2175	T3263	T3259	conj	%,%
R2176	T3264	T3265	punct	–,%
R2177	T3265	T3263	prep	%,%
R2178	T3266	T3265	nummod	20,%
R2179	T3267	T3263	punct	", ",%
R2180	T3268	T3263	cc	or,%
R2181	T3269	T3270	nummod	4,%
R2182	T3270	T3263	conj	%,%
R2183	T3271	T3272	punct	–,%
R2184	T3272	T3270	prep	%,%
R2185	T3273	T3272	nummod	20,%
R2186	T3274	T3260	compound	Tris,PAGE
R2187	T3275	T3260	punct	-,PAGE
R2188	T3276	T3260	compound	HCl,PAGE
R2189	T3277	T3257	pobj	gels,onto
R2190	T3278	T3279	punct	(,Laboratories
R2191	T3279	T3277	parataxis	Laboratories,gels
R2192	T3280	T3281	compound	Bio,Rad
R2193	T3281	T3279	compound	Rad,Laboratories
R2194	T3282	T3281	punct	-,Rad
R2195	T3283	T3279	punct	", ",Laboratories
R2196	T3284	T3279	npadvmod	Hercules,Laboratories
R2197	T3285	T3279	punct	", ",Laboratories
R2198	T3286	T3279	npadvmod	California,Laboratories
R2199	T3287	T3279	punct	", ",Laboratories
R2200	T3288	T3289	compound	United,States
R2201	T3289	T3279	npadvmod	States,Laboratories
R2202	T3290	T3279	punct	),Laboratories
R2203	T3291	T3241	cc	and,loaded
R2204	T3292	T3241	conj	electrophoresed,loaded
R2205	T3293	T3292	prep	for,electrophoresed
R2206	T3294	T3295	amod	several,hours
R2207	T3295	T3293	pobj	hours,for
R2208	T3296	T3292	prep	in,electrophoresed
R2209	T3297	T3298	nummod	1,sulfate
R2210	T3298	T3306	nmod	sulfate,buffer
R2211	T3299	T3297	punct	×,1
R2212	T3300	T3298	nmod	Tris,sulfate
R2213	T3301	T3298	punct	-,sulfate
R2214	T3302	T3298	nmod	glycine,sulfate
R2215	T3303	T3298	punct	–,sulfate
R2216	T3304	T3298	nmod	sodium,sulfate
R2217	T3305	T3298	nmod	dodecyl,sulfate
R2218	T3306	T3296	pobj	buffer,in
R2219	T3307	T3298	punct	(,sulfate
R2220	T3308	T3309	nummod	1,SDS
R2221	T3309	T3298	appos	SDS,sulfate
R2222	T3310	T3308	punct	×,1
R2223	T3311	T3309	compound	TG,SDS
R2224	T3312	T3309	punct	-,SDS
R2225	T3313	T3306	punct	),buffer
R2226	T3314	T3315	punct	(,Amresco
R2227	T3315	T3306	parataxis	Amresco,buffer
R2228	T3316	T3315	dep	6E10,Amresco
R2229	T3317	T3316	cc	and,6E10
R2230	T3318	T3316	conj	22C11,6E10
R2231	T3319	T3315	punct	;,Amresco
R2232	T3320	T3315	punct	", ",Amresco
R2233	T3321	T3315	npadvmod	Solon,Amresco
R2234	T3322	T3315	punct	", ",Amresco
R2235	T3323	T3315	npadvmod	Ohio,Amresco
R2236	T3324	T3315	punct	", ",Amresco
R2237	T3325	T3326	compound	United,States
R2238	T3326	T3315	npadvmod	States,Amresco
R2239	T3327	T3315	punct	),Amresco
R2240	T3328	T3306	cc	or,buffer
R2241	T3329	T3330	nummod	1,buffer
R2242	T3330	T3306	conj	buffer,buffer
R2243	T3331	T3329	punct	×,1
R2244	T3332	T3333	compound	Tris,SDS
R2245	T3333	T3330	compound	SDS,buffer
R2246	T3334	T3333	punct	-,SDS
R2247	T3335	T3333	compound	tricine,SDS
R2248	T3336	T3333	punct	-,SDS
R2249	T3337	T3338	punct	(,Invitrogen
R2250	T3338	T3330	parataxis	Invitrogen,buffer
R2251	T3339	T3338	dep	CT15,Invitrogen
R2252	T3340	T3338	punct	;,Invitrogen
R2253	T3341	T3338	punct	", ",Invitrogen
R2254	T3342	T3338	npadvmod	Carlsbad,Invitrogen
R2255	T3343	T3338	punct	", ",Invitrogen
R2256	T3344	T3338	npadvmod	California,Invitrogen
R2257	T3345	T3338	punct	", ",Invitrogen
R2258	T3346	T3347	compound	United,States
R2259	T3347	T3338	npadvmod	States,Invitrogen
R2260	T3348	T3338	punct	),Invitrogen
R2261	T3349	T3241	punct	.,loaded
R2262	T3351	T3352	nsubjpass	Proteins,transferred
R2263	T3353	T3352	auxpass	were,transferred
R2264	T3354	T3352	advmod	overnight,transferred
R2265	T3355	T3352	prep	to,transferred
R2266	T3356	T3357	nummod	0.45,μm
R2267	T3357	T3359	compound	μm,nitrocellulose
R2268	T3358	T3357	punct	-,μm
R2269	T3359	T3355	pobj	nitrocellulose,to
R2270	T3360	T3359	compound	Optitran,nitrocellulose
R2271	T3361	T3362	punct	(,Schleicher
R2272	T3362	T3359	parataxis	Schleicher,nitrocellulose
R2273	T3363	T3362	cc	and,Schleicher
R2274	T3364	T3362	conj	Schuell,Schleicher
R2275	T3365	T3362	punct	", ",Schleicher
R2276	T3366	T3362	npadvmod	Keene,Schleicher
R2277	T3367	T3362	punct	", ",Schleicher
R2278	T3368	T3369	compound	New,Hampshire
R2279	T3369	T3362	npadvmod	Hampshire,Schleicher
R2280	T3370	T3362	punct	", ",Schleicher
R2281	T3371	T3372	compound	United,States
R2282	T3372	T3362	npadvmod	States,Schleicher
R2283	T3373	T3362	punct	),Schleicher
R2284	T3374	T3352	prep	in,transferred
R2285	T3375	T3376	nummod	1,buffer
R2286	T3376	T3374	pobj	buffer,in
R2287	T3377	T3375	punct	×,1
R2288	T3378	T3376	compound	TG,buffer
R2289	T3379	T3380	punct	(,Amresco
R2290	T3380	T3376	parataxis	Amresco,buffer
R2291	T3381	T3380	punct	),Amresco
R2292	T3382	T3352	punct	.,transferred
R2293	T3384	T3385	nsubjpass	Blots,blocked
R2294	T3386	T3385	auxpass	were,blocked
R2295	T3387	T3385	prep	in,blocked
R2296	T3388	T3387	pobj	PBS,in
R2297	T3389	T3388	acl	containing,PBS
R2298	T3390	T3391	nummod	5,%
R2299	T3391	T3392	nmod	%,powder
R2300	T3392	T3389	dobj	powder,containing
R2301	T3393	T3394	amod	nonfat,milk
R2302	T3394	T3392	compound	milk,powder
R2303	T3395	T3394	amod	dry,milk
R2304	T3396	T3385	punct	", ",blocked
R2305	T3397	T3385	cc	and,blocked
R2306	T3398	T3385	conj	incubated,blocked
R2307	T3399	T3398	prep	for,incubated
R2308	T3400	T3401	nummod	3,h
R2309	T3401	T3399	pobj	h,for
R2310	T3402	T3398	prep	at,incubated
R2311	T3403	T3404	compound	room,temperature
R2312	T3404	T3402	pobj	temperature,at
R2313	T3405	T3398	prep	in,incubated
R2314	T3406	T3407	compound	blocking,solution
R2315	T3407	T3405	pobj	solution,in
R2316	T3408	T3407	prep	with,solution
R2317	T3409	T3408	pobj	one,with
R2318	T3410	T3409	prep	of,one
R2319	T3411	T3412	det	the,antibodies
R2320	T3412	T3410	pobj	antibodies,of
R2321	T3413	T3412	amod	following,antibodies
R2322	T3414	T3412	punct	: ,antibodies
R2323	T3415	T3416	nmod	mouse,22C11
R2324	T3416	T3412	appos	22C11,antibodies
R2325	T3417	T3416	amod	monoclonal,22C11
R2326	T3418	T3419	punct	(,17
R2328	T3420	T3421	amod	kind,gift
R2329	T3421	T3419	dep	gift,17
R2330	T3422	T3421	prep	of,gift
R2331	T3423	T3424	compound	Konrad,Beyreuther
R2332	T3424	T3422	pobj	Beyreuther,of
R2333	T3425	T3424	cc	and,Beyreuther
R2334	T3426	T3427	compound	Andreas,Weidemann
R2335	T3427	T3424	conj	Weidemann,Beyreuther
R2336	T3428	T3419	punct	;,17
R2337	T3429	T3419	punct	[,17
R2338	T3430	T3419	punct	],17
R2339	T3431	T3419	punct	),17
R2340	T3432	T3416	acl	diluted,22C11
R2341	T3433	T3432	npadvmod	1,diluted
R2342	T3434	T3435	punct	:,"1,000"
R2343	T3435	T3433	prep	"1,000",1
R2344	T3436	T3416	punct	", ",22C11
R2345	T3437	T3438	nmod	mouse,6E10
R2346	T3438	T3416	conj	6E10,22C11
R2347	T3439	T3438	amod	monoclonal,6E10
R2348	T3440	T3441	punct	(,Laboratories
R2349	T3441	T3438	parataxis	Laboratories,6E10
R2350	T3442	T3441	compound	Signet,Laboratories
R2351	T3443	T3441	punct	", ",Laboratories
R2352	T3444	T3441	npadvmod	Dedham,Laboratories
R2353	T3445	T3441	punct	", ",Laboratories
R2354	T3446	T3441	npadvmod	Massachusetts,Laboratories
R2355	T3447	T3441	punct	", ",Laboratories
R2356	T3448	T3449	compound	United,States
R2357	T3449	T3441	npadvmod	States,Laboratories
R2358	T3450	T3441	punct	),Laboratories
R2359	T3451	T3438	acl	diluted,6E10
R2360	T3452	T3451	npadvmod	1,diluted
R2361	T3453	T3454	punct	:,"2,500"
R2362	T3454	T3452	prep	"2,500",1
R2363	T3455	T3438	punct	", ",6E10
R2364	T3456	T3457	nmod	rabbit,hSOD1
R2365	T3457	T3438	conj	hSOD1,6E10
R2366	T3458	T3457	amod	polyclonal,hSOD1
R2367	T3459	T3457	amod	anti-superoxide,hSOD1
R2368	T3460	T3457	nmod	dismutase,hSOD1
R2369	T3461	T3457	nummod	1,hSOD1
R2370	T3462	T3457	punct	(,hSOD1
R2371	T3463	T3457	compound	m,hSOD1
R2372	T3464	T3457	punct	/,hSOD1
R2373	T3465	T3457	punct	),hSOD1
R2374	T3466	T3467	punct	[,18
R2375	T3467	T3457	parataxis	18,hSOD1
R2376	T3468	T3467	punct	],18
R2377	T3469	T3457	acl	diluted,hSOD1
R2378	T3470	T3469	npadvmod	1,diluted
R2379	T3471	T3472	punct	:,"2,500"
R2380	T3472	T3470	prep	"2,500",1
R2381	T3473	T3470	prep	to,1
R2382	T3474	T3473	pobj	1,to
R2383	T3475	T3476	punct	:,"4,000"
R2384	T3476	T3474	prep	"4,000",1
R2385	T3477	T3457	punct	", ",hSOD1
R2386	T3478	T3457	cc	or,hSOD1
R2387	T3479	T3480	nmod	rabbit,CT15
R2388	T3480	T3457	conj	CT15,hSOD1
R2389	T3481	T3480	amod	polyclonal,CT15
R2390	T3482	T3483	punct	(,gift
R2391	T3483	T3480	parataxis	gift,CT15
R2392	T3484	T3483	amod	kind,gift
R2393	T3485	T3483	prep	of,gift
R2394	T3486	T3487	compound	Ed,Koo
R2395	T3487	T3485	pobj	Koo,of
R2396	T3488	T3483	punct	;,gift
R2397	T3489	T3490	punct	[,19
R2398	T3490	T3483	parataxis	19,gift
R2399	T3491	T3490	punct	],19
R2400	T3492	T3483	punct	),gift
R2401	T3493	T3480	acl	diluted,CT15
R2402	T3494	T3493	npadvmod	1,diluted
R2403	T3495	T3496	punct	:,"1,000"
R2404	T3496	T3494	prep	"1,000",1
R2405	T3497	T3385	punct	.,blocked
R2406	T3499	T3500	advmod	Subsequently,washed
R2407	T3501	T3500	punct	", ",washed
R2408	T3502	T3503	det	the,blots
R2409	T3503	T3500	nsubjpass	blots,washed
R2410	T3504	T3500	auxpass	were,washed
R2411	T3505	T3500	prep	with,washed
R2412	T3506	T3505	pobj	PBS,with
R2413	T3507	T3506	acl	containing,PBS
R2414	T3508	T3509	nummod	0.1,%
R2415	T3509	T3510	compound	%,Tween
R2416	T3510	T3507	dobj	Tween,containing
R2417	T3511	T3510	punct	-,Tween
R2418	T3512	T3510	nummod	20,Tween
R2419	T3513	T3500	punct	", ",washed
R2420	T3514	T3500	cc	and,washed
R2421	T3515	T3516	advmod	then,incubated
R2422	T3516	T3500	conj	incubated,washed
R2423	T3517	T3516	prep	with,incubated
R2424	T3518	T3519	preconj	either,goat
R2425	T3519	T3520	nmod	goat,antibody
R2426	T3520	T3517	pobj	antibody,with
R2427	T3521	T3519	amod	anti-mouse,goat
R2428	T3522	T3519	punct	–,goat
R2429	T3523	T3519	cc	or,goat
R2430	T3524	T3519	conj	goat,goat
R2431	T3525	T3524	amod	anti-rabbit,goat
R2432	T3526	T3520	punct	–,antibody
R2433	T3527	T3528	npadvmod	HRP,conjugated
R2434	T3528	T3520	amod	conjugated,antibody
R2435	T3529	T3520	amod	secondary,antibody
R2436	T3530	T3520	acl	diluted,antibody
R2437	T3531	T3530	npadvmod	1,diluted
R2438	T3532	T3533	punct	:,"1,000"
R2439	T3533	T3531	prep	"1,000",1
R2440	T3534	T3530	prep	in,diluted
R2441	T3535	T3536	compound	blocking,solution
R2442	T3536	T3534	pobj	solution,in
R2443	T3537	T3500	punct	.,washed
R2444	T3539	T3540	prep	After,developed
R2445	T3541	T3542	amod	several,rinses
R2446	T3542	T3539	pobj	rinses,After
R2447	T3543	T3542	amod	additional,rinses
R2448	T3544	T3542	prep	in,rinses
R2449	T3545	T3544	pobj	PBS,in
R2450	T3546	T3542	prep	with,rinses
R2451	T3547	T3548	nummod	0.1,Tween
R2452	T3548	T3546	pobj	Tween,with
R2453	T3549	T3547	quantmod	%,0.1
R2454	T3550	T3548	punct	-,Tween
R2455	T3551	T3548	nummod	20,Tween
R2456	T3552	T3540	punct	", ",developed
R2457	T3553	T3540	nsubjpass	blots,developed
R2458	T3554	T3540	auxpass	were,developed
R2459	T3555	T3540	prep	with,developed
R2460	T3556	T3557	amod	enhanced,reagent
R2461	T3557	T3555	pobj	reagent,with
R2462	T3558	T3557	compound	chemiluminescence,reagent
R2463	T3559	T3540	cc	and,developed
R2464	T3560	T3540	conj	imaged,developed
R2465	T3561	T3560	prep	with,imaged
R2466	T3562	T3563	det	the,system
R2467	T3563	T3561	pobj	system,with
R2468	T3564	T3565	compound	Bio,Rad
R2469	T3565	T3567	compound	Rad,FX
R2470	T3566	T3565	punct	-,Rad
R2471	T3567	T3563	compound	FX,system
R2472	T3568	T3567	compound	Molecular,FX
R2473	T3569	T3567	compound	Imager,FX
R2474	T3570	T3540	punct	.,developed
R2475	T3572	T3573	compound	Staining,intensity
R2476	T3573	T3574	nsubjpass	intensity,quantified
R2477	T3575	T3573	prep	within,intensity
R2478	T3576	T3577	det	each,lane
R2479	T3577	T3575	pobj	lane,within
R2480	T3578	T3574	auxpass	was,quantified
R2481	T3579	T3574	advcl	using,quantified
R2482	T3580	T3581	det	the,software
R2483	T3581	T3579	dobj	software,using
R2484	T3582	T3581	nmod	Quantity,software
R2485	T3583	T3582	nummod	One,Quantity
R2486	T3584	T3585	compound	image,analysis
R2487	T3585	T3581	compound	analysis,software
R2488	T3586	T3587	punct	(,FX
R2489	T3587	T3581	parataxis	FX,software
R2490	T3588	T3589	compound	Molecular,Imager
R2491	T3589	T3587	compound	Imager,FX
R2492	T3590	T3587	punct	", ",FX
R2493	T3591	T3592	compound	Bio,Rad
R2494	T3592	T3594	compound	Rad,Laboratories
R2495	T3593	T3592	punct	-,Rad
R2496	T3594	T3587	npadvmod	Laboratories,FX
R2497	T3595	T3587	punct	),FX
R2498	T3596	T3574	punct	.,quantified
R2499	T3598	T3599	nsubjpass	Background,calculated
R2500	T3600	T3599	auxpass	was,calculated
R2501	T3601	T3599	prep	from,calculated
R2502	T3602	T3601	prep	across,from
R2503	T3603	T3604	det	the,image
R2504	T3604	T3602	pobj	image,across
R2505	T3605	T3599	cc	and,calculated
R2506	T3606	T3599	conj	subtracted,calculated
R2507	T3607	T3606	prep	from,subtracted
R2508	T3608	T3609	det	the,file
R2509	T3609	T3607	pobj	file,from
R2510	T3610	T3609	amod	entire,file
R2511	T3611	T3599	punct	.,calculated
R2512	T3613	T3614	det	The,intensity
R2513	T3614	T3616	nsubjpass	intensity,calculated
R2514	T3615	T3614	compound	signal,intensity
R2515	T3617	T3614	prep	for,intensity
R2516	T3618	T3619	det	each,band
R2517	T3619	T3617	pobj	band,for
R2518	T3620	T3621	punct	(,intensity
R2519	T3621	T3614	parataxis	intensity,intensity
R2520	T3622	T3621	amod	corrected,intensity
R2521	T3623	T3621	compound	signal,intensity
R2522	T3624	T3621	punct	×,intensity
R2523	T3625	T3626	compound	pixel,number
R2524	T3626	T3621	appos	number,intensity
R2525	T3627	T3621	punct	),intensity
R2526	T3628	T3616	auxpass	was,calculated
R2527	T3629	T3616	advmod	then,calculated
R2528	T3630	T3616	advcl	using,calculated
R2529	T3631	T3632	det	the,tool
R2530	T3632	T3630	dobj	tool,using
R2531	T3633	T3634	compound	Volume,report
R2532	T3634	T3632	compound	report,tool
R2533	T3635	T3616	punct	.,calculated
R2534	T3710	T3711	compound	Slot,Blot
R2535	T3711	T3712	compound	Blot,Analysis
R2536	T3713	T3712	compound	mRNA,Analysis
R2537	T3715	T3716	nummod	Five,micrograms
R2538	T3716	T3717	nsubjpass	micrograms,filtered
R2539	T3718	T3716	prep	per,micrograms
R2540	T3719	T3718	pobj	sample,per
R2541	T3720	T3719	prep	of,sample
R2542	T3721	T3722	amod	total,RNA
R2543	T3722	T3720	pobj	RNA,of
R2544	T3723	T3722	acl	extracted,RNA
R2545	T3724	T3723	prep	from,extracted
R2546	T3725	T3726	amod	fresh,frozen
R2547	T3726	T3728	amod	frozen,brain
R2548	T3727	T3726	punct	-,frozen
R2549	T3728	T3724	pobj	brain,from
R2550	T3729	T3728	punct	", ",brain
R2551	T3730	T3728	conj	liver,brain
R2552	T3731	T3730	punct	", ",liver
R2553	T3732	T3730	conj	kidney,liver
R2554	T3733	T3732	punct	", ",kidney
R2555	T3734	T3732	conj	heart,kidney
R2556	T3735	T3734	punct	", ",heart
R2557	T3736	T3734	conj	lung,heart
R2558	T3737	T3736	punct	", ",lung
R2559	T3738	T3736	conj	spleen,lung
R2560	T3739	T3738	punct	", ",spleen
R2561	T3740	T3738	cc	and,spleen
R2562	T3741	T3742	amod	skeletal,muscle
R2563	T3742	T3738	conj	muscle,spleen
R2564	T3743	T3717	auxpass	was,filtered
R2565	T3744	T3717	dep	vacuum,filtered
R2566	T3745	T3717	punct	-,filtered
R2567	T3746	T3717	prep	through,filtered
R2568	T3747	T3748	nummod	0.45,μm
R2569	T3748	T3750	compound	μm,nitrocellulose
R2570	T3749	T3748	punct	-,μm
R2571	T3750	T3746	pobj	nitrocellulose,through
R2572	T3751	T3750	compound	Optitran,nitrocellulose
R2573	T3752	T3717	punct	.,filtered
R2574	T3754	T3755	prep	After,cross-linked
R2575	T3756	T3757	amod	several,washes
R2576	T3757	T3754	pobj	washes,After
R2577	T3758	T3757	prep	through,washes
R2578	T3759	T3760	det	the,manifold
R2579	T3760	T3758	pobj	manifold,through
R2580	T3761	T3757	prep	with,washes
R2581	T3762	T3763	nummod	10,SSC
R2582	T3763	T3761	pobj	SSC,with
R2583	T3764	T3762	punct	×,10
R2584	T3765	T3755	punct	", ",cross-linked
R2585	T3766	T3755	nsubjpass	blots,cross-linked
R2586	T3767	T3755	auxpass	were,cross-linked
R2587	T3768	T3755	dep	UV,cross-linked
R2588	T3769	T3755	punct	-,cross-linked
R2589	T3770	T3755	cc	and,cross-linked
R2590	T3771	T3755	conj	probed,cross-linked
R2591	T3772	T3771	prep	with,probed
R2592	T3773	T3774	det	a,fragment
R2593	T3774	T3772	pobj	fragment,with
R2594	T3775	T3774	amod	radiolabeled,fragment
R2595	T3776	T3777	punct	∼,350
R2596	T3777	T3778	nummod	350,bp
R2597	T3778	T3774	compound	bp,fragment
R2598	T3779	T3778	punct	-,bp
R2599	T3780	T3781	compound	BglII,XhoI
R2600	T3781	T3774	compound	XhoI,fragment
R2601	T3782	T3781	punct	–,XhoI
R2602	T3783	T3774	compound	cDNA,fragment
R2603	T3784	T3774	prep	of,fragment
R2604	T3785	T3786	compound	mo,huAPP695
R2605	T3786	T3788	compound	huAPP695,cDNA
R2606	T3787	T3786	punct	/,huAPP695
R2607	T3788	T3784	pobj	cDNA,of
R2608	T3789	T3755	punct	.,cross-linked
R2609	T3791	T3792	prep	After,washed
R2610	T3793	T3791	pcomp	hybridizing,After
R2611	T3794	T3793	advmod	overnight,hybridizing
R2612	T3795	T3793	prep	at,hybridizing
R2613	T3796	T3797	nummod	65,°C
R2614	T3797	T3795	pobj	°C,at
R2615	T3798	T3793	prep	in,hybridizing
R2616	T3799	T3800	nummod	1,%
R2617	T3800	T3801	compound	%,BSA
R2618	T3801	T3798	pobj	BSA,in
R2619	T3802	T3801	punct	/,BSA
R2620	T3803	T3804	nummod	1,mM
R2621	T3804	T3805	compound	mM,EDTA
R2622	T3805	T3801	appos	EDTA,BSA
R2623	T3806	T3801	punct	/,BSA
R2624	T3807	T3808	nummod	0.5,M
R2625	T3808	T3809	compound	M,buffer
R2626	T3809	T3801	appos	buffer,BSA
R2627	T3810	T3809	compound	sodium,buffer
R2628	T3811	T3809	compound	phosphate,buffer
R2629	T3812	T3813	punct	(,pH
R2630	T3813	T3809	parataxis	pH,buffer
R2631	T3814	T3813	nummod	7.2,pH
R2632	T3815	T3813	punct	),pH
R2633	T3816	T3801	punct	/,BSA
R2634	T3817	T3818	nummod	7,%
R2635	T3818	T3819	compound	%,SDS
R2636	T3819	T3801	appos	SDS,BSA
R2637	T3820	T3821	punct	[,20
R2638	T3821	T3819	parataxis	20,SDS
R2639	T3822	T3821	punct	],20
R2640	T3823	T3792	punct	", ",washed
R2641	T3824	T3825	det	the,blots
R2642	T3825	T3792	nsubjpass	blots,washed
R2643	T3826	T3792	auxpass	were,washed
R2644	T3827	T3792	advmod	twice,washed
R2645	T3828	T3792	prep	at,washed
R2646	T3829	T3830	nummod	65,°C
R2647	T3830	T3828	pobj	°C,at
R2648	T3831	T3792	prep	for,washed
R2649	T3832	T3833	nummod	30,min
R2650	T3833	T3831	pobj	min,for
R2651	T3834	T3833	appos	each,min
R2652	T3835	T3792	prep	in,washed
R2653	T3836	T3837	nummod	0.1,%
R2654	T3837	T3838	compound	%,BSA
R2655	T3838	T3835	pobj	BSA,in
R2656	T3839	T3838	punct	/,BSA
R2657	T3840	T3841	nummod	1,mM
R2658	T3841	T3842	compound	mM,EDTA
R2659	T3842	T3838	appos	EDTA,BSA
R2660	T3843	T3838	punct	/,BSA
R2661	T3844	T3845	nummod	40,mM
R2662	T3845	T3846	compound	mM,buffer
R2663	T3846	T3838	appos	buffer,BSA
R2664	T3847	T3846	compound	sodium,buffer
R2665	T3848	T3846	compound	phosphate,buffer
R2666	T3849	T3850	punct	(,pH
R2667	T3850	T3846	parataxis	pH,buffer
R2668	T3851	T3850	nummod	7.2,pH
R2669	T3852	T3850	punct	),pH
R2670	T3853	T3838	punct	/,BSA
R2671	T3854	T3855	nummod	5,%
R2672	T3855	T3856	compound	%,SDS
R2673	T3856	T3838	appos	SDS,BSA
R2674	T3857	T3792	prep	before,washed
R2675	T3858	T3859	nummod	two,washes
R2676	T3859	T3857	pobj	washes,before
R2677	T3860	T3859	amod	final,washes
R2678	T3861	T3862	nummod	30,min
R2679	T3862	T3859	compound	min,washes
R2680	T3863	T3862	punct	-,min
R2681	T3864	T3859	prep	at,washes
R2682	T3865	T3866	nummod	65,°C
R2683	T3866	T3864	pobj	°C,at
R2684	T3867	T3859	prep	with,washes
R2685	T3868	T3869	nummod	1,mM
R2686	T3869	T3870	compound	mM,EDTA
R2687	T3870	T3867	pobj	EDTA,with
R2688	T3871	T3870	punct	/,EDTA
R2689	T3872	T3873	nummod	40,mM
R2690	T3873	T3874	compound	mM,buffer
R2691	T3874	T3870	appos	buffer,EDTA
R2692	T3875	T3874	compound	sodium,buffer
R2693	T3876	T3874	compound	phosphate,buffer
R2694	T3877	T3878	punct	(,pH
R2695	T3878	T3874	parataxis	pH,buffer
R2696	T3879	T3878	nummod	7.2,pH
R2697	T3880	T3878	punct	),pH
R2698	T3881	T3870	punct	/,EDTA
R2699	T3882	T3883	nummod	1,%
R2700	T3883	T3884	compound	%,SDS
R2701	T3884	T3870	appos	SDS,EDTA
R2702	T3885	T3792	punct	.,washed
R2703	T3887	T3888	nsubjpass	Blots,wrapped
R2704	T3889	T3888	auxpass	were,wrapped
R2705	T3890	T3888	advcl	wet,wrapped
R2706	T3891	T3888	cc	and,wrapped
R2707	T3892	T3888	conj	exposed,wrapped
R2708	T3893	T3892	prep	to,exposed
R2709	T3894	T3893	pobj	phosphorscreens,to
R2710	T3895	T3892	advmod	overnight,exposed
R2711	T3896	T3892	prep	at,exposed
R2712	T3897	T3898	compound	room,temperature
R2713	T3898	T3896	pobj	temperature,at
R2714	T3899	T3888	punct	.,wrapped
R2715	T4137	T4138	compound	Amyloid,Histology
R2716	T4140	T4141	nsubjpass	Mice,euthanized
R2717	T4142	T4141	auxpass	were,euthanized
R2718	T4143	T4141	prep	by,euthanized
R2719	T4144	T4145	compound	ether,inhalation
R2720	T4145	T4143	pobj	inhalation,by
R2721	T4146	T4141	cc	and,euthanized
R2722	T4147	T4148	nsubj	brains,removed
R2723	T4148	T4141	conj	removed,euthanized
R2724	T4149	T4148	prep	for,removed
R2725	T4150	T4151	compound	immersion,fixation
R2726	T4151	T4149	pobj	fixation,for
R2727	T4152	T4151	prep	in,fixation
R2728	T4153	T4154	nummod	4,%
R2729	T4154	T4155	compound	%,paraformaldehyde
R2730	T4155	T4152	pobj	paraformaldehyde,in
R2731	T4156	T4155	punct	/,paraformaldehyde
R2732	T4157	T4158	nummod	1,PBS
R2733	T4158	T4155	appos	PBS,paraformaldehyde
R2734	T4159	T4157	punct	×,1
R2735	T4160	T4148	punct	.,removed
R2736	T4162	T4163	prep	After,transferred
R2737	T4164	T4165	nummod	48,h
R2738	T4165	T4162	pobj	h,After
R2739	T4166	T4165	prep	in,h
R2740	T4167	T4166	pobj	fixative,in
R2741	T4168	T4165	prep	at,h
R2742	T4169	T4170	nummod	4,°C
R2743	T4170	T4168	pobj	°C,at
R2744	T4171	T4163	punct	", ",transferred
R2745	T4172	T4163	nsubjpass	brains,transferred
R2746	T4173	T4163	auxpass	were,transferred
R2747	T4174	T4163	prep	to,transferred
R2748	T4175	T4174	pobj	PBS,to
R2749	T4176	T4163	punct	", ",transferred
R2750	T4177	T4163	conj	dehydrated,transferred
R2751	T4178	T4177	prep	in,dehydrated
R2752	T4179	T4178	pobj	alcohols,in
R2753	T4180	T4177	punct	", ",dehydrated
R2754	T4181	T4177	conj	treated,dehydrated
R2755	T4182	T4181	prep	with,treated
R2756	T4183	T4184	compound	cedarwood,oil
R2757	T4184	T4182	pobj	oil,with
R2758	T4185	T4184	cc	and,oil
R2759	T4186	T4184	conj	methylsalicylate,oil
R2760	T4187	T4181	punct	", ",treated
R2761	T4188	T4181	cc	and,treated
R2762	T4189	T4181	conj	embedded,treated
R2763	T4190	T4189	prep	in,embedded
R2764	T4191	T4190	pobj	paraffin,in
R2765	T4192	T4189	prep	for,embedded
R2766	T4193	T4192	pobj	sectioning,for
R2767	T4194	T4163	punct	.,transferred
R2768	T4196	T4197	compound	Hirano,stain
R2769	T4198	T4197	compound	silver,stain
R2770	T4200	T4201	compound	Silver,histology
R2771	T4201	T4203	nsubjpass	histology,performed
R2772	T4202	T4201	compound	impregnation,histology
R2773	T4204	T4203	auxpass	was,performed
R2774	T4205	T4203	prep	on,performed
R2775	T4206	T4207	nummod	10,μm
R2776	T4207	T4209	nmod	μm,sections
R2777	T4208	T4207	punct	-,μm
R2778	T4209	T4205	pobj	sections,on
R2779	T4210	T4211	npadvmod	paraffin,embedded
R2780	T4211	T4209	amod	embedded,sections
R2781	T4212	T4211	punct	-,embedded
R2782	T4213	T4203	prep	by,performed
R2783	T4214	T4215	poss	Hirano,modification
R2784	T4215	T4213	pobj	modification,by
R2785	T4216	T4214	case	's,Hirano
R2786	T4217	T4215	prep	of,modification
R2787	T4218	T4219	det	the,method
R2788	T4219	T4217	pobj	method,of
R2789	T4220	T4219	compound	Bielschowsky,method
R2790	T4221	T4222	punct	[,21
R2791	T4222	T4203	parataxis	21,performed
R2792	T4223	T4222	punct	],21
R2793	T4224	T4203	punct	.,performed
R2794	T4226	T4227	advmod	Briefly,deparaffinized
R2795	T4228	T4227	punct	", ",deparaffinized
R2796	T4229	T4227	nsubjpass	sections,deparaffinized
R2797	T4230	T4227	auxpass	were,deparaffinized
R2798	T4231	T4227	prep	through,deparaffinized
R2799	T4232	T4231	pobj	xylene,through
R2800	T4233	T4232	cc	and,xylene
R2801	T4234	T4232	conj	alcohols,xylene
R2802	T4235	T4227	prep	into,deparaffinized
R2803	T4236	T4237	compound	tap,water
R2804	T4237	T4235	pobj	water,into
R2805	T4238	T4227	prep	before,deparaffinized
R2806	T4239	T4240	auxpass	being,placed
R2807	T4240	T4238	pcomp	placed,before
R2808	T4241	T4240	prep	into,placed
R2809	T4242	T4243	amod	fresh,solution
R2810	T4243	T4241	pobj	solution,into
R2811	T4244	T4245	nummod	20,%
R2812	T4245	T4243	compound	%,solution
R2813	T4246	T4243	compound	silver,solution
R2814	T4247	T4243	compound	nitrate,solution
R2815	T4248	T4240	prep	for,placed
R2816	T4249	T4250	nummod	20,min
R2817	T4250	T4248	pobj	min,for
R2818	T4251	T4227	punct	.,deparaffinized
R2819	T4253	T4254	prep	After,immersed
R2820	T4255	T4256	auxpass	being,washed
R2821	T4256	T4253	pcomp	washed,After
R2822	T4257	T4256	advmod	thoroughly,washed
R2823	T4258	T4256	prep	with,washed
R2824	T4259	T4260	amod	distilled,water
R2825	T4260	T4258	pobj	water,with
R2826	T4261	T4254	punct	", ",immersed
R2827	T4262	T4254	nsubjpass	slides,immersed
R2828	T4263	T4254	auxpass	were,immersed
R2829	T4264	T4254	prep	in,immersed
R2830	T4265	T4266	nummod	20,%
R2831	T4266	T4267	compound	%,solution
R2832	T4267	T4264	pobj	solution,in
R2833	T4268	T4269	compound	silver,nitrate
R2834	T4269	T4267	compound	nitrate,solution
R2835	T4270	T4267	acl	titrated,solution
R2836	T4271	T4270	prep	with,titrated
R2837	T4272	T4273	amod	fresh,hydroxide
R2838	T4273	T4271	pobj	hydroxide,with
R2839	T4274	T4273	compound	ammonium,hydroxide
R2840	T4275	T4254	punct	.,immersed
R2841	T4277	T4278	prep	After,washed
R2842	T4279	T4280	nummod	20,min
R2843	T4280	T4277	pobj	min,After
R2844	T4281	T4278	punct	", ",washed
R2845	T4282	T4278	nsubjpass	slides,washed
R2846	T4283	T4278	auxpass	were,washed
R2847	T4284	T4278	prep	with,washed
R2848	T4285	T4286	compound	ammonia,water
R2849	T4286	T4284	pobj	water,with
R2850	T4287	T4278	prep	before,washed
R2851	T4288	T4289	auxpass	being,developed
R2852	T4289	T4287	pcomp	developed,before
R2853	T4290	T4289	advmod	individually,developed
R2854	T4291	T4289	prep	with,developed
R2855	T4292	T4293	nummod	100,μl
R2856	T4293	T4291	pobj	μl,with
R2857	T4294	T4293	prep	of,μl
R2858	T4295	T4294	pobj	developer,of
R2859	T4296	T4297	punct	(,ml
R2860	T4297	T4295	parataxis	ml,developer
R2861	T4298	T4297	nummod	20,ml
R2862	T4299	T4297	prep	of,ml
R2863	T4300	T4301	nummod	37,%
R2864	T4301	T4302	compound	%,formaldehyde
R2865	T4302	T4299	pobj	formaldehyde,of
R2866	T4303	T4297	punct	", ",ml
R2867	T4304	T4305	nummod	100,ml
R2868	T4305	T4297	conj	ml,ml
R2869	T4306	T4305	prep	of,ml
R2870	T4307	T4308	amod	distilled,water
R2871	T4308	T4306	pobj	water,of
R2872	T4309	T4305	punct	", ",ml
R2873	T4310	T4311	nummod	50,μl
R2874	T4311	T4305	conj	μl,ml
R2875	T4312	T4311	prep	of,μl
R2876	T4313	T4314	amod	concentrated,acid
R2877	T4314	T4312	pobj	acid,of
R2878	T4315	T4314	amod	nitric,acid
R2879	T4316	T4311	punct	", ",μl
R2880	T4317	T4311	cc	and,μl
R2881	T4318	T4319	nummod	0.5,g
R2882	T4319	T4311	conj	g,μl
R2883	T4320	T4319	prep	of,g
R2884	T4321	T4322	amod	citric,acid
R2885	T4322	T4320	pobj	acid,of
R2886	T4323	T4297	punct	),ml
R2887	T4324	T4293	acl	added,μl
R2888	T4325	T4324	prep	to,added
R2889	T4326	T4327	nummod	50,ml
R2890	T4327	T4325	pobj	ml,to
R2891	T4328	T4327	prep	of,ml
R2892	T4329	T4330	amod	titrated,solution
R2893	T4330	T4328	pobj	solution,of
R2894	T4331	T4332	compound	silver,nitrate
R2895	T4332	T4330	compound	nitrate,solution
R2896	T4333	T4278	punct	.,washed
R2897	T4335	T4336	nsubjpass	Slides,rinsed
R2898	T4337	T4336	auxpass	were,rinsed
R2899	T4338	T4336	advmod	then,rinsed
R2900	T4339	T4336	prep	in,rinsed
R2901	T4340	T4341	compound	tap,water
R2902	T4341	T4339	pobj	water,in
R2903	T4342	T4336	punct	", ",rinsed
R2904	T4343	T4336	conj	fixed,rinsed
R2905	T4344	T4343	prep	in,fixed
R2906	T4345	T4346	nummod	5,%
R2907	T4346	T4347	compound	%,thiosulfate
R2908	T4347	T4344	pobj	thiosulfate,in
R2909	T4348	T4347	compound	sodium,thiosulfate
R2910	T4349	T4343	punct	", ",fixed
R2911	T4350	T4343	cc	and,fixed
R2912	T4351	T4343	conj	dehydrated,fixed
R2913	T4352	T4351	prep	through,dehydrated
R2914	T4353	T4352	pobj	alcohols,through
R2915	T4354	T4353	cc	and,alcohols
R2916	T4355	T4353	conj	xylene,alcohols
R2917	T4356	T4336	punct	.,rinsed
R2918	T4358	T4359	compound	Thioflavin,S
R2919	T4359	T4361	compound	S,staining
R2920	T4360	T4359	punct	-,S
R2921	T4363	T4364	prep	Following,performed
R2922	T4365	T4363	pobj	deparaffinization,Following
R2923	T4366	T4365	prep	of,deparaffinization
R2924	T4367	T4366	pobj	sections,of
R2925	T4368	T4365	prep	through,deparaffinization
R2926	T4369	T4368	pobj	xylene,through
R2927	T4370	T4369	cc	and,xylene
R2928	T4371	T4369	conj	alcohols,xylene
R2929	T4372	T4364	punct	", ",performed
R2930	T4373	T4374	compound	amyloid,impregnation
R2931	T4374	T4364	nsubjpass	impregnation,performed
R2932	T4375	T4374	prep	with,impregnation
R2933	T4376	T4377	compound	thioflavin,S
R2934	T4377	T4375	pobj	S,with
R2935	T4378	T4377	punct	-,S
R2936	T4379	T4364	auxpass	was,performed
R2937	T4380	T4364	prep	according,performed
R2938	T4381	T4380	prep	to,according
R2939	T4382	T4383	det	the,modification
R2940	T4383	T4381	pobj	modification,to
R2941	T4384	T4383	compound	Guntern,modification
R2942	T4385	T4383	prep	of,modification
R2943	T4386	T4387	det	the,protocol
R2944	T4387	T4385	pobj	protocol,of
R2945	T4388	T4387	amod	standard,protocol
R2946	T4389	T4364	punct	.,performed
R2947	T4391	T4392	nsubjpass	Slides,washed
R2948	T4393	T4391	acl	holding,Slides
R2949	T4394	T4395	nummod	10,μm
R2950	T4395	T4397	compound	μm,sections
R2951	T4396	T4395	punct	-,μm
R2952	T4397	T4393	dobj	sections,holding
R2953	T4398	T4397	compound	paraffin,sections
R2954	T4399	T4392	auxpass	were,washed
R2955	T4400	T4392	advmod	twice,washed
R2956	T4401	T4392	prep	in,washed
R2957	T4402	T4403	amod	distilled,water
R2958	T4403	T4401	pobj	water,in
R2959	T4404	T4392	punct	", ",washed
R2960	T4405	T4406	advmod	then,immersed
R2961	T4406	T4392	dep	immersed,washed
R2962	T4407	T4406	prep	for,immersed
R2963	T4408	T4409	nummod	5,min
R2964	T4409	T4407	pobj	min,for
R2965	T4410	T4406	prep	in,immersed
R2966	T4411	T4412	det	a,solution
R2967	T4412	T4410	pobj	solution,in
R2968	T4413	T4414	nummod	0.25,%
R2969	T4414	T4412	compound	%,solution
R2970	T4415	T4412	compound	potassium,solution
R2971	T4416	T4412	compound	permanganate,solution
R2972	T4417	T4406	punct	", ",immersed
R2973	T4418	T4406	advcl	followed,immersed
R2974	T4419	T4418	agent	by,followed
R2975	T4420	T4421	nummod	5,min
R2976	T4421	T4419	pobj	min,by
R2977	T4422	T4421	prep	in,min
R2978	T4423	T4424	det	a,solution
R2979	T4424	T4422	pobj	solution,in
R2980	T4425	T4426	nummod	1,%
R2981	T4426	T4427	nmod	%,metabisulfate
R2982	T4427	T4424	nmod	metabisulfate,solution
R2983	T4428	T4427	nmod	potassium,metabisulfate
R2984	T4429	T4427	punct	/,metabisulfate
R2985	T4430	T4431	nummod	1,%
R2986	T4431	T4432	nmod	%,acid
R2987	T4432	T4427	appos	acid,metabisulfate
R2988	T4433	T4432	amod	oxalic,acid
R2989	T4434	T4392	punct	.,washed
R2990	T4436	T4437	prep	After,placed
R2991	T4438	T4439	det	this,preparation
R2992	T4439	T4436	pobj	preparation,After
R2993	T4440	T4437	punct	", ",placed
R2994	T4441	T4437	nsubjpass	slides,placed
R2995	T4442	T4437	auxpass	were,placed
R2996	T4443	T4437	prep	into,placed
R2997	T4444	T4445	det	a,solution
R2998	T4445	T4443	pobj	solution,into
R2999	T4446	T4445	amod	filtered,solution
R3000	T4447	T4445	amod	aqueous,solution
R3001	T4448	T4449	nummod	0.02,%
R3002	T4449	T4445	compound	%,solution
R3003	T4450	T4451	compound	thioflavin,S
R3004	T4451	T4445	compound	S,solution
R3005	T4452	T4451	punct	-,S
R3006	T4453	T4454	punct	(,Schmid
R3007	T4454	T4445	parataxis	Schmid,solution
R3008	T4455	T4456	compound	Chroma,Gesellschaft
R3009	T4456	T4454	compound	Gesellschaft,Schmid
R3010	T4457	T4456	punct	-,Gesellschaft
R3011	T4458	T4454	punct	", ",Schmid
R3012	T4459	T4454	npadvmod	Kongen,Schmid
R3013	T4460	T4454	punct	", ",Schmid
R3014	T4461	T4454	npadvmod	Germany,Schmid
R3015	T4462	T4454	punct	),Schmid
R3016	T4463	T4437	prep	for,placed
R3017	T4464	T4465	nummod	8,min
R3018	T4465	T4463	pobj	min,for
R3019	T4466	T4437	punct	.,placed
R3020	T4468	T4469	amod	Excess,stain
R3021	T4469	T4470	nsubjpass	stain,removed
R3022	T4471	T4470	auxpass	was,removed
R3023	T4472	T4470	agent	by,removed
R3024	T4473	T4474	nummod	two,rinses
R3025	T4474	T4472	pobj	rinses,by
R3026	T4475	T4474	amod	brief,rinses
R3027	T4476	T4474	prep	in,rinses
R3028	T4477	T4478	nummod	80,%
R3029	T4478	T4479	compound	%,ethanol
R3030	T4479	T4476	pobj	ethanol,in
R3031	T4480	T4474	punct	", ",rinses
R3032	T4481	T4482	advmod	then,two
R3033	T4482	T4474	appos	two,rinses
R3034	T4483	T4482	prep	in,two
R3035	T4484	T4485	amod	distilled,water
R3036	T4485	T4483	pobj	water,in
R3037	T4486	T4474	punct	", ",rinses
R3038	T4487	T4488	prep	after,finished
R3039	T4488	T4474	relcl	finished,rinses
R3040	T4489	T4487	pobj	which,after
R3041	T4490	T4488	nsubjpass	slides,finished
R3042	T4491	T4488	auxpass	were,finished
R3043	T4492	T4488	prep	in,finished
R3044	T4493	T4494	amod	aqueous,medium
R3045	T4494	T4492	pobj	medium,in
R3046	T4495	T4494	compound	mounting,medium
R3047	T4496	T4488	prep	for,finished
R3048	T4497	T4498	compound	florescence,photomicrography
R3049	T4498	T4496	pobj	photomicrography,for
R3050	T4499	T4470	punct	.,removed
R3051	T4502	T4501	punct	", ",Ubiquitin
R3052	T4503	T4504	amod	glial,protein
R3053	T4504	T4501	conj	protein,Ubiquitin
R3054	T4505	T4504	amod	fibrillary,protein
R3055	T4506	T4504	amod	acidic,protein
R3056	T4507	T4504	punct	", ",protein
R3057	T4508	T4504	cc	and,protein
R3058	T4509	T4510	compound	Aβ,immunohistochemistry
R3059	T4510	T4504	conj	immunohistochemistry,protein
R3060	T4512	T4513	amod	Prior,to
R3061	T4513	T4514	prep	to,deparaffinized
R3062	T4515	T4513	pobj	immunostaining,to
R3063	T4516	T4514	punct	", ",deparaffinized
R3064	T4517	T4514	nsubjpass	slides,deparaffinized
R3065	T4518	T4514	auxpass	were,deparaffinized
R3066	T4519	T4514	prep	by,deparaffinized
R3067	T4520	T4521	compound	oven,heating
R3068	T4521	T4519	pobj	heating,by
R3069	T4522	T4514	advcl	followed,deparaffinized
R3070	T4523	T4522	agent	by,followed
R3071	T4524	T4523	pobj	immersion,by
R3072	T4525	T4524	prep	in,immersion
R3073	T4526	T4525	pobj	xylene,in
R3074	T4527	T4514	punct	.,deparaffinized
R3075	T4529	T4530	prep	After,quenched
R3076	T4531	T4529	pobj	rehydration,After
R3077	T4532	T4531	prep	through,rehydration
R3078	T4533	T4534	amod	graded,alcohols
R3079	T4534	T4532	pobj	alcohols,through
R3080	T4535	T4531	prep	into,rehydration
R3081	T4536	T4537	compound	tap,water
R3082	T4537	T4535	pobj	water,into
R3083	T4538	T4530	punct	", ",quenched
R3084	T4539	T4540	amod	endogenous,activity
R3085	T4540	T4530	nsubjpass	activity,quenched
R3086	T4541	T4540	compound	peroxidase,activity
R3087	T4542	T4530	auxpass	was,quenched
R3088	T4543	T4530	agent	by,quenched
R3089	T4544	T4543	pobj	incubation,by
R3090	T4545	T4544	prep	with,incubation
R3091	T4546	T4547	nummod	3,%
R3092	T4547	T4548	compound	%,peroxide
R3093	T4548	T4545	pobj	peroxide,with
R3094	T4549	T4548	compound	hydrogen,peroxide
R3095	T4550	T4544	prep	in,incubation
R3096	T4551	T4550	pobj	methanol,in
R3097	T4552	T4530	punct	.,quenched
R3098	T4554	T4555	nsubjpass	Slides,microwaved
R3099	T4556	T4555	auxpass	were,microwaved
R3100	T4557	T4555	prep	for,microwaved
R3101	T4558	T4559	quantmod	5,7
R3102	T4559	T4561	nummod	7,min
R3103	T4560	T4559	punct	–,7
R3104	T4561	T4557	pobj	min,for
R3105	T4562	T4555	prep	in,microwaved
R3106	T4563	T4562	pobj	water,in
R3107	T4564	T4555	punct	", ",microwaved
R3108	T4565	T4555	dep	cooled,microwaved
R3109	T4566	T4565	prep	for,cooled
R3110	T4567	T4568	nummod	5,min
R3111	T4568	T4566	pobj	min,for
R3112	T4569	T4555	punct	", ",microwaved
R3113	T4570	T4571	advmod	then,washed
R3114	T4571	T4555	dep	washed,microwaved
R3115	T4572	T4571	prep	in,washed
R3116	T4573	T4572	pobj	TBS,in
R3117	T4574	T4555	punct	.,microwaved
R3118	T4576	T4577	amod	Nonspecific,staining
R3119	T4577	T4578	nsubjpass	staining,blocked
R3120	T4579	T4578	auxpass	was,blocked
R3121	T4580	T4578	prep	for,blocked
R3122	T4581	T4582	nummod	1,h
R3123	T4582	T4580	pobj	h,for
R3124	T4583	T4578	prep	with,blocked
R3125	T4584	T4585	nummod	3,%
R3126	T4585	T4586	nmod	%,serum
R3127	T4586	T4583	pobj	serum,with
R3128	T4587	T4586	amod	normal,serum
R3129	T4588	T4586	compound	goat,serum
R3130	T4589	T4586	cc	and,serum
R3131	T4590	T4591	nummod	0.1,%
R3132	T4591	T4592	compound	%,X
R3133	T4592	T4586	conj	X,serum
R3134	T4593	T4592	compound	Triton,X
R3135	T4594	T4592	punct	-,X
R3136	T4595	T4592	nummod	100,X
R3137	T4596	T4586	prep	in,serum
R3138	T4597	T4596	pobj	TBS,in
R3139	T4598	T4578	punct	.,blocked
R3140	T4600	T4601	nsubjpass	Slides,placed
R3141	T4602	T4601	auxpass	were,placed
R3142	T4603	T4601	advmod	then,placed
R3143	T4604	T4601	prep	into,placed
R3144	T4605	T4606	amod	primary,antibody
R3145	T4606	T4604	pobj	antibody,into
R3146	T4607	T4608	punct	(,antibodies
R3147	T4608	T4606	parataxis	antibodies,antibody
R3148	T4609	T4610	nmod	rabbit,antibody
R3149	T4610	T4608	dep	antibody,antibodies
R3150	T4611	T4610	amod	anti-Aβ,antibody
R3151	T4612	T4610	nmod	peptide,antibody
R3152	T4613	T4610	amod	polyclonal,antibody
R3153	T4614	T4610	punct	", ",antibody
R3154	T4615	T4616	compound	Zymed,Laboratories
R3155	T4616	T4610	npadvmod	Laboratories,antibody
R3156	T4617	T4610	punct	", ",antibody
R3157	T4618	T4619	compound	South,Francisco
R3158	T4619	T4610	npadvmod	Francisco,antibody
R3159	T4620	T4619	compound	San,Francisco
R3160	T4621	T4610	punct	", ",antibody
R3161	T4622	T4610	npadvmod	California,antibody
R3162	T4623	T4610	punct	", ",antibody
R3163	T4624	T4625	compound	United,States
R3164	T4625	T4610	npadvmod	States,antibody
R3165	T4626	T4608	punct	;,antibodies
R3166	T4627	T4608	nmod	rabbit,antibodies
R3167	T4628	T4627	amod	anti-ubiquitin,rabbit
R3168	T4629	T4627	cc	and,rabbit
R3169	T4630	T4627	conj	rabbit,rabbit
R3170	T4631	T4630	amod	anti–glial,rabbit
R3171	T4632	T4633	amod	fibrillary,protein
R3172	T4633	T4608	nmod	protein,antibodies
R3173	T4634	T4633	amod	acidic,protein
R3174	T4635	T4633	punct	(,protein
R3175	T4636	T4633	appos	GFAP,protein
R3176	T4637	T4608	punct	),antibodies
R3177	T4638	T4608	amod	polyclonal,antibodies
R3178	T4639	T4608	punct	", ",antibodies
R3179	T4640	T4608	npadvmod	Dako,antibodies
R3180	T4641	T4608	punct	", ",antibodies
R3181	T4642	T4608	npadvmod	Carpinteria,antibodies
R3182	T4643	T4608	punct	", ",antibodies
R3183	T4644	T4608	npadvmod	California,antibodies
R3184	T4645	T4608	punct	", ",antibodies
R3185	T4646	T4647	compound	United,States
R3186	T4647	T4608	npadvmod	States,antibodies
R3187	T4648	T4608	punct	),antibodies
R3188	T4649	T4606	acl	diluted,antibody
R3189	T4650	T4649	npadvmod	1,diluted
R3190	T4651	T4652	punct	:,500
R3191	T4652	T4650	prep	500,1
R3192	T4653	T4649	prep	in,diluted
R3193	T4654	T4653	pobj	TBS,in
R3194	T4655	T4649	prep	with,diluted
R3195	T4656	T4657	nummod	2,%
R3196	T4657	T4658	nmod	%,serum
R3197	T4658	T4655	pobj	serum,with
R3198	T4659	T4658	amod	normal,serum
R3199	T4660	T4658	compound	goat,serum
R3200	T4661	T4601	cc	and,placed
R3201	T4662	T4601	conj	incubated,placed
R3202	T4663	T4662	advmod	overnight,incubated
R3203	T4664	T4662	prep	at,incubated
R3204	T4665	T4666	compound	room,temperature
R3205	T4666	T4664	pobj	temperature,at
R3206	T4667	T4601	punct	.,placed
R3207	T4669	T4670	prep	After,incubated
R3208	T4671	T4672	auxpass	being,washed
R3209	T4672	T4669	pcomp	washed,After
R3210	T4673	T4672	prep	of,washed
R3211	T4674	T4675	amod	excess,antibody
R3212	T4675	T4673	pobj	antibody,of
R3213	T4676	T4675	amod	primary,antibody
R3214	T4677	T4672	prep	with,washed
R3215	T4678	T4679	amod	several,changes
R3216	T4679	T4677	pobj	changes,with
R3217	T4680	T4679	prep	of,changes
R3218	T4681	T4680	pobj	TBS,of
R3219	T4682	T4670	punct	", ",incubated
R3220	T4683	T4670	nsubjpass	slides,incubated
R3221	T4684	T4670	auxpass	were,incubated
R3222	T4685	T4670	prep	with,incubated
R3223	T4686	T4687	preconj	either,system
R3224	T4687	T4685	pobj	system,with
R3225	T4688	T4687	det	the,system
R3226	T4689	T4690	nmod	Vectastain,Elite
R3227	T4690	T4687	nmod	Elite,system
R3228	T4691	T4687	amod	anti-rabbit,system
R3229	T4692	T4687	amod	secondary,system
R3230	T4693	T4694	punct	(,Laboratories
R3231	T4694	T4687	parataxis	Laboratories,system
R3232	T4695	T4694	amod	anti-Aβ,Laboratories
R3233	T4696	T4694	punct	;,Laboratories
R3234	T4697	T4694	compound	Vector,Laboratories
R3235	T4698	T4694	punct	", ",Laboratories
R3236	T4699	T4694	npadvmod	Burlingame,Laboratories
R3237	T4700	T4694	punct	", ",Laboratories
R3238	T4701	T4694	npadvmod	California,Laboratories
R3239	T4702	T4694	punct	", ",Laboratories
R3240	T4703	T4704	compound	United,States
R3241	T4704	T4694	npadvmod	States,Laboratories
R3242	T4705	T4694	punct	),Laboratories
R3243	T4706	T4687	cc	or,system
R3244	T4707	T4708	nmod	peroxidase,reagents
R3245	T4708	T4687	conj	reagents,system
R3246	T4709	T4707	punct	/,peroxidase
R3247	T4710	T4707	amod	anti-peroxidase,peroxidase
R3248	T4711	T4712	punct	(,Monoclonals
R3249	T4712	T4708	parataxis	Monoclonals,reagents
R3250	T4713	T4712	amod	anti-ubitquitin,Monoclonals
R3251	T4714	T4713	cc	and,anti-ubitquitin
R3252	T4715	T4713	conj	anti-GFAP,anti-ubitquitin
R3253	T4716	T4712	punct	;,Monoclonals
R3254	T4717	T4712	compound	Sternberger,Monoclonals
R3255	T4718	T4712	punct	", ",Monoclonals
R3256	T4719	T4712	npadvmod	Lutherville,Monoclonals
R3257	T4720	T4712	punct	", ",Monoclonals
R3258	T4721	T4712	npadvmod	Maryland,Monoclonals
R3259	T4722	T4712	punct	", ",Monoclonals
R3260	T4723	T4724	compound	United,States
R3261	T4724	T4712	npadvmod	States,Monoclonals
R3262	T4725	T4712	punct	),Monoclonals
R3263	T4726	T4670	prep	according,incubated
R3264	T4727	T4726	prep	to,according
R3265	T4728	T4729	det	the,manufacturers
R3266	T4729	T4730	poss	manufacturers,directions
R3267	T4730	T4727	pobj	directions,to
R3268	T4731	T4729	case	',manufacturers
R3269	T4732	T4670	punct	.,incubated
R3270	T4734	T4735	compound	Antibody,binding
R3271	T4735	T4736	nsubjpass	binding,visualized
R3272	T4737	T4736	auxpass	was,visualized
R3273	T4738	T4736	prep	with,visualized
R3274	T4739	T4738	pobj	diaminobenzidene,with
R3275	T4740	T4736	punct	", ",visualized
R3276	T4741	T4736	cc	and,visualized
R3277	T4742	T4743	nsubjpass	sections,counterstained
R3278	T4743	T4736	conj	counterstained,visualized
R3279	T4744	T4743	auxpass	were,counterstained
R3280	T4745	T4743	prep	with,counterstained
R3281	T4746	T4745	pobj	hematoxylin,with
R3282	T4747	T4743	punct	.,counterstained
R3283	T4787	T4788	compound	Filter,Assay
R3284	T4789	T4788	compound	Trap,Assay
R3285	T4791	T4792	det	An,aliquot
R3286	T4792	T4793	nsubjpass	aliquot,solubilized
R3287	T4794	T4792	prep	of,aliquot
R3288	T4795	T4796	det	each,homogenate
R3289	T4796	T4794	pobj	homogenate,of
R3290	T4797	T4796	amod	cortical,homogenate
R3291	T4798	T4796	acl	used,homogenate
R3292	T4799	T4798	prep	for,used
R3293	T4800	T4801	compound	Western,blotting
R3294	T4801	T4799	pobj	blotting,for
R3295	T4802	T4798	advmod	above,used
R3296	T4803	T4793	auxpass	was,solubilized
R3297	T4804	T4793	advmod	partially,solubilized
R3298	T4805	T4793	prep	by,solubilized
R3299	T4806	T4807	det	the,addition
R3300	T4807	T4805	pobj	addition,by
R3301	T4808	T4807	prep	of,addition
R3302	T4809	T4808	pobj	SDS,of
R3303	T4810	T4793	prep	to,solubilized
R3304	T4811	T4812	det	a,concentration
R3305	T4812	T4810	pobj	concentration,to
R3306	T4813	T4812	amod	final,concentration
R3307	T4814	T4812	prep	of,concentration
R3308	T4815	T4816	nummod	1,%
R3309	T4816	T4814	pobj	%,of
R3310	T4817	T4793	punct	.,solubilized
R3311	T4819	T4820	amod	Serial,dilutions
R3312	T4820	T4824	nsubjpass	dilutions,made
R3313	T4821	T4820	nummod	1,dilutions
R3314	T4822	T4823	punct	:,2
R3315	T4823	T4821	prep	2,1
R3316	T4825	T4824	auxpass	were,made
R3317	T4826	T4824	prep	with,made
R3318	T4827	T4828	nummod	1,PBS
R3319	T4828	T4826	pobj	PBS,with
R3320	T4829	T4827	punct	×,1
R3321	T4830	T4828	punct	/,PBS
R3322	T4831	T4832	nummod	1,%
R3323	T4832	T4833	compound	%,SDS
R3324	T4833	T4828	appos	SDS,PBS
R3325	T4834	T4824	punct	", ",made
R3326	T4835	T4824	cc	and,made
R3327	T4836	T4837	nummod	100,μl
R3328	T4837	T4838	nsubjpass	μl,filtered
R3329	T4838	T4824	conj	filtered,made
R3330	T4839	T4837	prep	of,μl
R3331	T4840	T4841	det	each,dilution
R3332	T4841	T4839	pobj	dilution,of
R3333	T4842	T4838	auxpass	was,filtered
R3334	T4843	T4838	advmod	then,filtered
R3335	T4844	T4838	dep	vacuum,filtered
R3336	T4845	T4838	punct	-,filtered
R3337	T4846	T4838	prep	through,filtered
R3338	T4847	T4848	det	a,membrane
R3339	T4848	T4846	pobj	membrane,through
R3340	T4849	T4848	amod	pre-wet,membrane
R3341	T4850	T4851	nummod	0.22,μm
R3342	T4851	T4848	compound	μm,membrane
R3343	T4852	T4851	punct	-,μm
R3344	T4853	T4848	compound	cellulose,membrane
R3345	T4854	T4848	compound	acetate,membrane
R3346	T4855	T4856	punct	(,Schleicher
R3347	T4856	T4848	parataxis	Schleicher,membrane
R3348	T4857	T4856	cc	and,Schleicher
R3349	T4858	T4856	conj	Schuell,Schleicher
R3350	T4859	T4856	punct	),Schleicher
R3351	T4860	T4861	punct	[,22
R3352	T4861	T4838	parataxis	22,filtered
R3353	T4862	T4861	punct	],22
R3354	T4863	T4838	punct	.,filtered
R3355	T4865	T4866	det	Each,well
R3356	T4866	T4867	nsubjpass	well,washed
R3357	T4868	T4867	auxpass	was,washed
R3358	T4869	T4870	amod	several,times
R3359	T4870	T4867	dobj	times,washed
R3360	T4871	T4867	prep	with,washed
R3361	T4872	T4871	pobj	PBS,with
R3362	T4873	T4867	punct	", ",washed
R3363	T4874	T4875	prep	after,incubated
R3364	T4875	T4867	advcl	incubated,washed
R3365	T4876	T4874	pobj	which,after
R3366	T4877	T4875	nsubjpass	blots,incubated
R3367	T4878	T4875	auxpass	were,incubated
R3368	T4879	T4875	advmod	overnight,incubated
R3369	T4880	T4875	prep	with,incubated
R3370	T4881	T4882	amod	polyclonal,antibody
R3371	T4882	T4880	pobj	antibody,with
R3372	T4883	T4882	amod	anti-Aβ,antibody
R3373	T4884	T4885	punct	(,Laboratories
R3374	T4885	T4882	parataxis	Laboratories,antibody
R3375	T4886	T4885	compound	Zymed,Laboratories
R3376	T4887	T4885	punct	),Laboratories
R3377	T4888	T4882	acl	diluted,antibody
R3378	T4889	T4888	npadvmod	1,diluted
R3379	T4890	T4891	punct	:,600
R3380	T4891	T4889	prep	600,1
R3381	T4892	T4888	prep	in,diluted
R3382	T4893	T4894	det	a,solution
R3383	T4894	T4892	pobj	solution,in
R3384	T4895	T4894	compound	blocking,solution
R3385	T4896	T4894	prep	of,solution
R3386	T4897	T4898	nummod	1,TBS
R3387	T4898	T4900	nmod	TBS,powder
R3388	T4899	T4897	punct	×,1
R3389	T4900	T4896	pobj	powder,of
R3390	T4901	T4898	punct	/,TBS
R3391	T4902	T4903	nummod	5,%
R3392	T4903	T4904	nmod	%,milk
R3393	T4904	T4898	appos	milk,TBS
R3394	T4905	T4904	amod	nonfat,milk
R3395	T4906	T4904	amod	dry,milk
R3396	T4907	T4867	punct	.,washed
R3397	T4909	T4910	prep	After,incubated
R3398	T4911	T4909	pcomp	washing,After
R3399	T4912	T4913	det	the,blots
R3400	T4913	T4911	dobj	blots,washing
R3401	T4914	T4915	nummod	three,times
R3402	T4915	T4911	npadvmod	times,washing
R3403	T4916	T4911	prep	for,washing
R3404	T4917	T4918	nummod	10,min
R3405	T4918	T4916	pobj	min,for
R3406	T4919	T4918	appos	each,min
R3407	T4920	T4911	prep	in,washing
R3408	T4921	T4922	nummod	1,TBS
R3409	T4922	T4920	pobj	TBS,in
R3410	T4923	T4921	punct	×,1
R3411	T4924	T4922	punct	/,TBS
R3412	T4925	T4926	nummod	0.1,%
R3413	T4926	T4927	compound	%,Tween
R3414	T4927	T4922	appos	Tween,TBS
R3415	T4928	T4927	punct	-,Tween
R3416	T4929	T4927	nummod	20,Tween
R3417	T4930	T4910	punct	", ",incubated
R3418	T4931	T4932	det	the,membrane
R3419	T4932	T4910	nsubjpass	membrane,incubated
R3420	T4933	T4910	auxpass	was,incubated
R3421	T4934	T4910	prep	for,incubated
R3422	T4935	T4936	nummod	1,h
R3423	T4936	T4934	pobj	h,for
R3424	T4937	T4910	prep	with,incubated
R3425	T4938	T4939	npadvmod	HRP,conjugated
R3426	T4939	T4941	amod	conjugated,A
R3427	T4940	T4939	punct	-,conjugated
R3428	T4941	T4937	pobj	A,with
R3429	T4942	T4941	compound	protein,A
R3430	T4943	T4944	punct	(,Sigma
R3431	T4944	T4941	parataxis	Sigma,A
R3432	T4945	T4944	punct	),Sigma
R3433	T4946	T4941	acl	diluted,A
R3434	T4947	T4946	dobj	1,diluted
R3435	T4948	T4949	punct	:,"5,000"
R3436	T4949	T4947	prep	"5,000",1
R3437	T4950	T4946	prep	in,diluted
R3438	T4951	T4952	compound	blocking,solution
R3439	T4952	T4950	pobj	solution,in
R3440	T4953	T4910	punct	.,incubated
R3441	T4955	T4956	det	The,membranes
R3442	T4956	T4957	nsubjpass	membranes,washed
R3443	T4958	T4957	auxpass	were,washed
R3444	T4959	T4957	advmod	again,washed
R3445	T4960	T4961	nummod	three,times
R3446	T4961	T4957	npadvmod	times,washed
R3447	T4962	T4957	prep	with,washed
R3448	T4963	T4964	nummod	1,TBS
R3449	T4964	T4962	pobj	TBS,with
R3450	T4965	T4963	punct	×,1
R3451	T4966	T4964	punct	/,TBS
R3452	T4967	T4968	nummod	0.1,%
R3453	T4968	T4969	compound	%,Tween
R3454	T4969	T4964	appos	Tween,TBS
R3455	T4970	T4969	punct	-,Tween
R3456	T4971	T4969	nummod	20,Tween
R3457	T4972	T4957	punct	", ",washed
R3458	T4973	T4974	mark	before,detected
R3459	T4974	T4957	advcl	detected,washed
R3460	T4975	T4976	compound	antibody,binding
R3461	T4976	T4974	nsubjpass	binding,detected
R3462	T4977	T4974	auxpass	was,detected
R3463	T4978	T4974	prep	with,detected
R3464	T4979	T4980	amod	enhanced,chemiluminescence
R3465	T4980	T4978	pobj	chemiluminescence,with
R3466	T4981	T4982	punct	(,PerkinElmer
R3467	T4982	T4974	parataxis	PerkinElmer,detected
R3468	T4983	T4982	punct	", ",PerkinElmer
R3469	T4984	T4982	npadvmod	Boston,PerkinElmer
R3470	T4985	T4982	punct	", ",PerkinElmer
R3471	T4986	T4982	npadvmod	Massachusetts,PerkinElmer
R3472	T4987	T4982	punct	", ",PerkinElmer
R3473	T4988	T4989	compound	United,States
R3474	T4989	T4982	npadvmod	States,PerkinElmer
R3475	T4990	T4982	punct	),PerkinElmer
R3476	T4991	T4957	punct	.,washed
R3477	T4993	T4994	amod	Digital,images
R3478	T4994	T4995	nsubjpass	images,captured
R3479	T4996	T4994	prep	of,images
R3480	T4997	T4998	det	each,blot
R3481	T4998	T4996	pobj	blot,of
R3482	T4999	T4995	auxpass	were,captured
R3483	T5000	T4995	prep	with,captured
R3484	T5001	T5002	det	a,system
R3485	T5002	T5000	pobj	system,with
R3486	T5003	T5004	amod	Molecular,Imager
R3487	T5004	T5005	compound	Imager,FX
R3488	T5005	T5002	compound	FX,system
R3489	T5006	T5007	compound	gel,documentation
R3490	T5007	T5002	compound	documentation,system
R3491	T5008	T4995	punct	", ",captured
R3492	T5009	T4995	cc	and,captured
R3493	T5010	T5011	det	the,intensity
R3494	T5011	T5012	nsubjpass	intensity,quantified
R3495	T5012	T4995	conj	quantified,captured
R3496	T5013	T5011	prep	of,intensity
R3497	T5014	T5015	compound	Aβ,staining
R3498	T5015	T5013	pobj	staining,of
R3499	T5016	T5012	auxpass	was,quantified
R3500	T5017	T5012	advcl	using,quantified
R3501	T5018	T5019	nmod	Quantity,software
R3502	T5019	T5017	dobj	software,using
R3503	T5020	T5018	nummod	One,Quantity
R3504	T5021	T5022	compound	image,analysis
R3505	T5022	T5019	compound	analysis,software
R3506	T5023	T5012	punct	.,quantified
R3507	T5075	T5076	compound	Aβ,ELISA
R3508	T5078	T5079	det	An,aliquot
R3509	T5079	T5080	nsubjpass	aliquot,subjected
R3510	T5081	T5079	prep	of,aliquot
R3511	T5082	T5083	amod	cortical,homogenate
R3512	T5083	T5081	pobj	homogenate,of
R3513	T5084	T5083	acl	generated,homogenate
R3514	T5085	T5084	prep	for,generated
R3515	T5086	T5087	compound	Western,analysis
R3516	T5087	T5085	pobj	analysis,for
R3517	T5088	T5087	acl	described,analysis
R3518	T5089	T5088	advmod	above,described
R3519	T5090	T5080	auxpass	was,subjected
R3520	T5091	T5080	prep	to,subjected
R3521	T5092	T5093	det	a,extraction
R3522	T5093	T5091	pobj	extraction,to
R3523	T5094	T5095	nummod	three,step
R3524	T5095	T5093	nmod	step,extraction
R3525	T5096	T5095	punct	-,step
R3526	T5097	T5093	amod	sequential,extraction
R3527	T5098	T5080	advcl	using,subjected
R3528	T5099	T5098	dobj	PBS,using
R3529	T5100	T5099	punct	", ",PBS
R3530	T5101	T5102	nummod	2,%
R3531	T5102	T5103	compound	%,SDS
R3532	T5103	T5099	conj	SDS,PBS
R3533	T5104	T5103	punct	", ",SDS
R3534	T5105	T5103	cc	and,SDS
R3535	T5106	T5107	nummod	70,%
R3536	T5107	T5103	conj	%,SDS
R3537	T5108	T5109	amod	formic,acid
R3538	T5109	T5107	appos	acid,%
R3539	T5110	T5109	punct	(,acid
R3540	T5111	T5109	appos	FA,acid
R3541	T5112	T5080	punct	),subjected
R3542	T5113	T5080	punct	.,subjected
R3543	T5115	T5116	prep	At,sonicated
R3544	T5117	T5118	det	each,step
R3545	T5118	T5115	pobj	step,At
R3546	T5119	T5116	punct	", ",sonicated
R3547	T5120	T5121	det	the,sample
R3548	T5121	T5116	nsubjpass	sample,sonicated
R3549	T5122	T5116	auxpass	was,sonicated
R3550	T5123	T5116	prep	in,sonicated
R3551	T5124	T5125	amod	appropriate,buffer
R3552	T5125	T5123	pobj	buffer,in
R3553	T5126	T5116	cc	and,sonicated
R3554	T5127	T5116	conj	centrifuged,sonicated
R3555	T5128	T5127	prep	at,centrifuged
R3556	T5129	T5128	pobj	"100,000g",at
R3557	T5130	T5127	prep	for,centrifuged
R3558	T5131	T5132	nummod	30,min
R3559	T5132	T5130	pobj	min,for
R3560	T5133	T5134	punct	(,samples
R3561	T5134	T5132	parataxis	samples,min
R3562	T5135	T5136	quantmod	1,1.5
R3563	T5136	T5139	nummod	1.5,mo
R3564	T5137	T5136	punct	-,1.5
R3565	T5138	T5136	quantmod	to,1.5
R3566	T5139	T5134	compound	mo,samples
R3567	T5140	T5139	punct	-,mo
R3568	T5141	T5134	punct	),samples
R3569	T5142	T5132	cc	or,min
R3570	T5143	T5144	nummod	60,min
R3571	T5144	T5132	conj	min,min
R3572	T5145	T5146	punct	(,samples
R3573	T5146	T5144	parataxis	samples,min
R3574	T5147	T5148	quantmod	6,12
R3575	T5148	T5151	nummod	12,mo
R3576	T5149	T5148	punct	-,12
R3577	T5150	T5148	quantmod	to,12
R3578	T5151	T5146	compound	mo,samples
R3579	T5152	T5151	punct	-,mo
R3580	T5153	T5146	punct	),samples
R3581	T5154	T5127	prep	at,centrifuged
R3582	T5155	T5156	nummod	4,°C
R3583	T5156	T5154	pobj	°C,at
R3584	T5157	T5158	mark	as,described
R3585	T5158	T5127	advcl	described,centrifuged
R3586	T5159	T5158	advmod	previously,described
R3587	T5160	T5161	punct	[,23
R3588	T5161	T5127	parataxis	23,centrifuged
R3589	T5162	T5163	punct	–,25
R3590	T5163	T5161	prep	25,23
R3591	T5164	T5161	punct	],23
R3592	T5165	T5116	punct	.,sonicated
R3593	T5167	T5168	det	The,supernatant
R3594	T5168	T5169	nsubjpass	supernatant,removed
R3595	T5170	T5169	auxpass	was,removed
R3596	T5171	T5169	prep	for,removed
R3597	T5172	T5171	pobj	analysis,for
R3598	T5173	T5169	punct	", ",removed
R3599	T5174	T5169	cc	and,removed
R3600	T5175	T5176	det	the,pellet
R3601	T5176	T5177	nsubjpass	pellet,sonicated
R3602	T5177	T5169	conj	sonicated,removed
R3603	T5178	T5177	auxpass	was,sonicated
R3604	T5179	T5177	prep	in,sonicated
R3605	T5180	T5181	det	the,solution
R3606	T5181	T5179	pobj	solution,in
R3607	T5182	T5181	amod	next,solution
R3608	T5183	T5177	prep	in,sonicated
R3609	T5184	T5183	pobj	sequence,in
R3610	T5185	T5177	punct	.,sonicated
R3611	T5187	T5188	det	The,extracts
R3612	T5188	T5192	nsubjpass	extracts,diluted
R3613	T5189	T5190	nummod	2,%
R3614	T5190	T5188	compound	%,extracts
R3615	T5191	T5188	compound	SDS,extracts
R3616	T5193	T5192	auxpass	were,diluted
R3617	T5194	T5192	prep	in,diluted
R3618	T5195	T5196	compound	EC,buffer
R3619	T5196	T5194	pobj	buffer,in
R3620	T5197	T5192	punct	", ",diluted
R3621	T5198	T5192	cc	and,diluted
R3622	T5199	T5200	det	the,extracts
R3623	T5200	T5202	nsubj	extracts,neutralized
R3624	T5201	T5200	compound	FA,extracts
R3625	T5202	T5192	conj	neutralized,diluted
R3626	T5203	T5202	prep	with,neutralized
R3627	T5204	T5205	compound	1M,buffer
R3628	T5205	T5203	pobj	buffer,with
R3629	T5206	T5205	compound	Tris,buffer
R3630	T5207	T5205	punct	-,buffer
R3631	T5208	T5205	compound	phosphate,buffer
R3632	T5209	T5210	punct	(,pH
R3633	T5210	T5205	parataxis	pH,buffer
R3634	T5211	T5210	nummod	11,pH
R3635	T5212	T5210	punct	),pH
R3636	T5213	T5214	advmod	then,diluted
R3637	T5214	T5202	dep	diluted,neutralized
R3638	T5215	T5214	prep	with,diluted
R3639	T5216	T5217	compound	EC,buffer
R3640	T5217	T5215	pobj	buffer,with
R3641	T5218	T5219	amod	prior,to
R3642	T5219	T5214	prep	to,diluted
R3643	T5220	T5219	pobj	testing,to
R3644	T5221	T5202	punct	.,neutralized
R3645	T5223	T5224	amod	Human,Aβ
R3646	T5224	T5225	nsubjpass	Aβ,measured
R3647	T5226	T5225	auxpass	was,measured
R3648	T5227	T5225	prep	in,measured
R3649	T5228	T5229	det	each,fraction
R3650	T5229	T5227	pobj	fraction,in
R3651	T5230	T5225	advcl	using,measured
R3652	T5231	T5230	dobj	BAN50,using
R3653	T5232	T5230	prep	for,using
R3654	T5233	T5232	pobj	capture,for
R3655	T5234	T5235	punct	(,16
R3656	T5235	T5233	parataxis	16,capture
R3657	T5236	T5235	compound	epitope,16
R3658	T5237	T5235	compound	Aβ1,16
R3659	T5238	T5235	punct	–,16
R3660	T5239	T5235	punct	),16
R3661	T5240	T5230	cc	and,using
R3662	T5241	T5230	conj	BA27,using
R3663	T5242	T5241	cc	and,BA27
R3664	T5243	T5241	conj	BC05,BA27
R3665	T5244	T5241	prep	for,BA27
R3666	T5245	T5244	pobj	detection,for
R3667	T5246	T5247	punct	(,Aβ40
R3668	T5247	T5245	parataxis	Aβ40,detection
R3669	T5248	T5247	cc	and,Aβ40
R3670	T5249	T5247	conj	Aβ42,Aβ40
R3671	T5250	T5247	punct	", ",Aβ40
R3672	T5251	T5247	advmod	respectively,Aβ40
R3673	T5252	T5247	punct	),Aβ40
R3674	T5253	T5254	punct	(,Industries
R3675	T5254	T5245	parataxis	Industries,detection
R3676	T5255	T5254	compound	Takeda,Industries
R3677	T5256	T5254	compound	Chemical,Industries
R3678	T5257	T5254	punct	", ",Industries
R3679	T5258	T5254	npadvmod	Osaka,Industries
R3680	T5259	T5254	punct	", ",Industries
R3681	T5260	T5254	npadvmod	Japan,Industries
R3682	T5261	T5254	punct	),Industries
R3683	T5262	T5225	punct	.,measured
R3684	T5264	T5265	amod	Total,Aβ
R3685	T5265	T5266	nsubjpass	Aβ,measured
R3686	T5267	T5268	punct	(,samples
R3688	T5269	T5268	dep	mouse,samples
R3689	T5270	T5269	punct	+,mouse
R3690	T5271	T5269	amod	human,mouse
R3691	T5272	T5268	punct	;,samples
R3692	T5273	T5274	quantmod	1,1.5
R3693	T5274	T5277	nummod	1.5,mo
R3694	T5275	T5274	punct	-,1.5
R3695	T5276	T5274	quantmod	to,1.5
R3696	T5277	T5268	compound	mo,samples
R3697	T5278	T5277	punct	-,mo
R3698	T5279	T5268	advmod	only,samples
R3699	T5280	T5268	punct	),samples
R3700	T5281	T5266	auxpass	was,measured
R3701	T5282	T5266	prep	in,measured
R3702	T5283	T5284	det	each,fraction
R3703	T5284	T5282	pobj	fraction,in
R3704	T5285	T5266	advcl	using,measured
R3705	T5286	T5285	dobj	BNT77,using
R3706	T5287	T5285	prep	for,using
R3707	T5288	T5287	pobj	capture,for
R3708	T5289	T5290	punct	(,Aβ11
R3709	T5290	T5288	parataxis	Aβ11,capture
R3710	T5291	T5290	compound	epitope,Aβ11
R3711	T5292	T5290	punct	–,Aβ11
R3712	T5293	T5290	nummod	28,Aβ11
R3713	T5294	T5290	punct	),Aβ11
R3714	T5295	T5285	cc	and,using
R3715	T5296	T5285	conj	BA27,using
R3716	T5297	T5296	cc	and,BA27
R3717	T5298	T5296	conj	BC05,BA27
R3718	T5299	T5296	prep	for,BA27
R3719	T5300	T5299	pobj	detection,for
R3720	T5301	T5266	punct	.,measured
R3721	T5303	T5304	det	All,values
R3722	T5304	T5305	nsubjpass	values,calculated
R3723	T5306	T5305	auxpass	were,calculated
R3724	T5307	T5305	prep	as,calculated
R3725	T5308	T5307	pobj	picomoles,as
R3726	T5309	T5308	prep	per,picomoles
R3727	T5310	T5309	pobj	gram,per
R3728	T5311	T5305	prep	based,calculated
R3729	T5312	T5311	prep	on,based
R3730	T5313	T5314	det	the,weight
R3731	T5314	T5312	pobj	weight,on
R3732	T5315	T5314	amod	initial,weight
R3733	T5316	T5314	prep	of,weight
R3734	T5317	T5318	amod	cortical,tissue
R3735	T5318	T5316	pobj	tissue,of
R3736	T5319	T5305	punct	.,calculated
R3737	T5339	T5340	compound	Activity,Monitoring
R3738	T5342	T5343	amod	Daily,activity
R3739	T5343	T5345	nsubjpass	activity,studied
R3740	T5344	T5343	amod	basal,activity
R3741	T5346	T5345	auxpass	was,studied
R3742	T5347	T5345	prep	in,studied
R3743	T5348	T5349	nummod	28,mice
R9	T146	T144	pobj	Model,in
R10	T147	T146	amod	Transgenic,Model
R15	T156	T159	nsubj	secretases,been
R16	T157	T156	nmod	proteases,secretases
R17	T158	T156	punct	(,secretases
R22	T164	T166	nmod	β,peptide
R23	T165	T164	punct	-,β
R24	T166	T162	dobj	peptide,cleave
R25	T167	T164	punct	(,β
R26	T168	T164	appos	Aβ,β
R27	T169	T166	punct	),peptide
R30	T172	T170	pobj	protein,from
R31	T173	T172	compound	amyloid,protein
R32	T174	T172	compound	precursor,protein
R55	T199	T196	ccomp	facilitate,been
R56	T200	T199	csubj	reducing,facilitate
R57	T201	T202	compound	Aβ,production
R58	T202	T200	dobj	production,reducing
R59	T203	T200	prep	in,reducing
R60	T204	T205	det	the,brain
R61	T205	T203	pobj	brain,in
R62	T206	T199	punct	", ",facilitate
R63	T207	T208	advmod	even,after
R64	T208	T199	prep	after,facilitate
R65	T209	T210	det	the,onset
R66	T210	T208	pobj	onset,after
R67	T211	T210	prep	of,onset
R68	T212	T213	amod	clinical,symptoms
R69	T213	T211	pobj	symptoms,of
R70	T214	T210	cc	and,onset
R71	T215	T216	det	the,development
R72	T216	T210	conj	development,onset
R73	T217	T216	prep	of,development
R74	T218	T219	amod	associated,pathology
R75	T219	T217	pobj	pathology,of
R76	T220	T199	punct	", ",facilitate
R77	T221	T199	aux	will,facilitate
R92	T238	T235	nsubjpass	studies,performed
R93	T239	T240	amod	long,term
R95	T241	T240	punct	-,term
R115	T265	T262	dobj	model,generated
R116	T266	T265	amod	transgenic,model
R117	T267	T265	compound	mouse,model
R119	T269	T265	relcl	mimics,model
R120	T270	T269	advmod	genetically,mimics
R141	T293	T291	relcl	regulated,vector
R142	T294	T293	aux	can,regulated
R143	T295	T293	auxpass	be,regulated
R152	T306	T308	compound	level,expression
R153	T307	T306	punct	-,level
R159	T313	T301	dobj	pathology,induces
R160	T314	T313	compound	amyloid,pathology
R164	T320	T318	ccomp	inhibits,show
R165	T321	T322	compound	doxycycline,administration
R166	T322	T320	nsubj	administration,inhibits
R168	T324	T320	dobj	expression,inhibits
R169	T325	T324	compound	APP,expression
R172	T328	T330	nummod	95,%
R173	T329	T328	quantmod	than,95
R189	T347	T345	pobj	synthesis,of
R190	T348	T347	compound	Aβ,synthesis
R214	T374	T370	advcl	require,be
R215	T375	T376	det	the,plaques
R216	T376	T374	nsubj	plaques,require
R222	T382	T374	advcl	assemble,require
R223	T383	T382	aux	to,assemble
R227	T389	T386	relcl	suppressed,Mice
R228	T390	T388	pobj	which,in
R229	T391	T392	compound	APP,synthesis
R230	T392	T389	nsubjpass	synthesis,suppressed
R231	T393	T389	auxpass	was,suppressed
R234	T396	T399	nummod	6,mo
R235	T397	T396	amod	long,6
R236	T398	T396	quantmod	as,6
R245	T407	T387	dobj	load,retain
R246	T408	T407	amod	considerable,load
R247	T409	T407	compound	amyloid,load
R259	T425	T423	ccomp	are,demonstrates
R260	T426	T427	compound	amyloid,lesions
R261	T427	T425	nsubj	lesions,are
R262	T428	T427	prep	in,lesions
R263	T429	T430	amod	transgenic,mice
R264	T430	T428	pobj	mice,in
R279	T447	T445	advcl	halt,suggest
R280	T448	T447	csubj	arresting,halt
R281	T449	T450	compound	Aβ,production
R282	T450	T448	dobj	production,arresting
R283	T451	T448	prep	in,arresting
R284	T452	T451	pobj	patients,in
R285	T453	T452	prep	with,patients
R286	T454	T455	compound	Alzheimer,disease
R287	T455	T453	pobj	disease,with
R288	T456	T447	aux	should,halt
R296	T464	T447	conj	be,halt
R297	T465	T466	amod	early,treatment
R298	T466	T464	nsubj	treatment,be
R299	T467	T464	aux	may,be
R303	T471	T464	advcl	appears,be
R304	T472	T471	nsubj	it,appears
R306	T474	T471	ccomp	require,appears
R307	T475	T476	compound	amyloid,deposits
R308	T476	T474	nsubj	deposits,require
R309	T477	T474	punct	", ",require
R310	T478	T479	mark	once,formed
R311	T479	T474	advcl	formed,require
R312	T480	T474	punct	", ",require
R313	T481	T474	aux	will,require
R319	T136	T137	amod	Persistent,Amyloidosis
R323	T706	T707	quantmod	Over,a
R324	T707	T708	nummod	a,decade
R325	T708	T709	npadvmod	decade,ago
R326	T709	T710	advmod	ago,predicted
R327	T711	T712	det	the,hypothesis
R328	T712	T710	nsubj	hypothesis,predicted
R329	T713	T714	compound	amyloid,cascade
R330	T714	T712	compound	cascade,hypothesis
R331	T715	T716	mark	that,lead
R332	T716	T710	ccomp	lead,predicted
R333	T717	T718	amod	increased,levels
R334	T718	T716	nsubj	levels,lead
R335	T719	T718	prep	of,levels
R336	T720	T721	nmod	amyloid,β
R337	T721	T723	nmod	β,peptide
R338	T722	T721	punct	-,β
R339	T723	T719	pobj	peptide,of
R340	T724	T721	punct	(,β
R341	T725	T721	appos	Aβ,β
R342	T726	T723	punct	),peptide
R343	T727	T716	prep	to,lead
R344	T728	T729	amod	secondary,pathologies
R345	T729	T727	pobj	pathologies,to
R346	T730	T731	dep	that,culminate
R347	T731	T729	relcl	culminate,pathologies
R348	T732	T731	advmod	ultimately,culminate
R349	T733	T731	prep	in,culminate
R350	T734	T735	det	the,onset
R351	T735	T733	pobj	onset,in
R352	T736	T735	prep	of,onset
R353	T737	T738	compound	Alzheimer,disease
R354	T738	T736	pobj	disease,of
R355	T739	T738	punct	(,disease
R356	T740	T738	appos	AD,disease
R357	T741	T716	punct	),lead
R358	T742	T743	punct	[,1
R359	T743	T716	parataxis	1,lead
R360	T744	T743	punct	],1
R361	T745	T710	punct	.,predicted
R362	T747	T748	amod	Early,support
R363	T748	T749	nsubj	support,came
R364	T750	T748	prep	for,support
R365	T751	T752	det	this,hypothesis
R366	T752	T750	pobj	hypothesis,for
R367	T753	T749	prep	from,came
R368	T754	T755	amod	genetic,studies
R369	T755	T753	pobj	studies,from
R370	T756	T755	acl	linking,studies
R371	T757	T758	amod	early,onset
R372	T758	T760	compound	onset,AD
R373	T759	T758	punct	-,onset
R374	T760	T756	dobj	AD,linking
R375	T761	T756	prep	to,linking
R376	T762	T761	pobj	mutations,to
R377	T763	T762	prep	in,mutations
R378	T764	T765	det	the,protein
R379	T765	T763	pobj	protein,in
R380	T766	T765	compound	amyloid,protein
R381	T767	T765	compound	precursor,protein
R382	T768	T765	punct	(,protein
R383	T769	T765	appos	APP,protein
R384	T770	T765	punct	),protein
R385	T771	T765	punct	", ",protein
R386	T772	T773	prep	from,derived
R387	T773	T765	relcl	derived,protein
R388	T774	T772	pobj	which,from
R389	T775	T773	nsubjpass	Aβ,derived
R390	T776	T773	auxpass	is,derived
R391	T777	T765	punct	", ",protein
R392	T778	T765	cc	and,protein
R393	T779	T780	nmod	presenilins,1
R394	T780	T765	conj	1,protein
R395	T781	T780	cc	and,1
R396	T782	T780	conj	2,1
R397	T783	T780	punct	", ",1
R398	T784	T785	dep	which,are
R399	T785	T780	relcl	are,1
R400	T786	T787	amod	interchangeable,components
R401	T787	T785	attr	components,are
R402	T788	T787	prep	of,components
R403	T789	T790	det	a,complex
R404	T790	T788	pobj	complex,of
R405	T791	T790	compound	endoprotease,complex
R406	T792	T793	dep	that,releases
R407	T793	T790	relcl	releases,complex
R408	T794	T793	dobj	Aβ,releases
R409	T795	T793	prep	from,releases
R410	T796	T795	pobj	APP,from
R411	T797	T798	punct	(,see
R412	T798	T793	parataxis	see,releases
R413	T799	T798	prep	for,see
R414	T800	T799	pobj	review,for
R415	T801	T798	punct	[,see
R416	T802	T803	nummod	2,3
R417	T803	T798	dobj	3,see
R418	T804	T803	punct	",",3
R419	T805	T798	punct	],see
R420	T806	T798	punct	),see
R421	T807	T749	punct	.,came
R422	T809	T810	mark	If,initiates
R423	T810	T818	advcl	initiates,arrest
R424	T811	T810	punct	", ",initiates
R425	T812	T813	mark	as,predicted
R426	T813	T810	advcl	predicted,initiates
R427	T814	T810	punct	", ",initiates
R428	T815	T810	nsubj	overproduction,initiates
R429	T816	T815	prep	of,overproduction
R430	T817	T816	pobj	Aβ,of
R431	T819	T820	det	the,cascade
R432	T820	T810	dobj	cascade,initiates
R433	T821	T820	prep	of,cascade
R434	T822	T821	pobj	events,of
R435	T823	T822	acl	leading,events
R436	T824	T823	prep	to,leading
R437	T825	T824	pobj	disease,to
R438	T826	T818	punct	", ",arrest
R439	T827	T818	advmod	then,arrest
R440	T828	T829	amod	therapeutic,strategies
R441	T829	T818	nsubj	strategies,arrest
R442	T830	T831	dep	that,lower
R443	T831	T829	relcl	lower,strategies
R444	T832	T833	compound	Aβ,levels
R445	T833	T831	dobj	levels,lower
R446	T834	T818	aux	should,arrest
R447	T835	T818	preconj	either,arrest
R448	T836	T818	cc	or,arrest
R449	T837	T818	conj	reverse,arrest
R450	T838	T839	det	the,progression
R451	T839	T837	dobj	progression,reverse
R452	T840	T839	prep	from,progression
R453	T841	T840	pobj	peptide,from
R454	T842	T840	prep	to,from
R455	T843	T842	pobj	dementia,to
R456	T844	T818	punct	.,arrest
R457	T846	T847	amod	Early,evidence
R458	T847	T848	nsubj	evidence,suggested
R459	T849	T847	prep	from,evidence
R460	T850	T851	amod	clinical,trials
R461	T851	T849	pobj	trials,from
R462	T852	T851	prep	of,trials
R463	T853	T854	npadvmod	antibody,mediated
R464	T854	T856	amod	mediated,clearance
R465	T855	T854	punct	-,mediated
R466	T856	T852	pobj	clearance,of
R467	T857	T856	punct	", ",clearance
R468	T858	T856	appos	one,clearance
R469	T859	T858	prep	of,one
R470	T860	T861	det	the,approaches
R472	T862	T861	amod	first,approaches
R473	T863	T864	npadvmod	Aβ,lowering
R474	T864	T861	amod	lowering,approaches
R475	T865	T864	punct	-,lowering
R476	T866	T861	acl	tested,approaches
R477	T867	T866	prep	in,tested
R478	T868	T867	pobj	humans,in
R479	T869	T848	punct	", ",suggested
R480	T870	T871	mark	that,be
R481	T871	T848	ccomp	be,suggested
R482	T872	T871	nsubj	treatments,be
R483	T873	T872	acl	designed,treatments
R484	T874	T875	aux	to,reduce
R485	T875	T873	advcl	reduce,designed
R486	T876	T877	compound	amyloid,burden
R487	T877	T875	dobj	burden,reduce
R488	T878	T871	aux	may,be
R489	T879	T871	advmod	indeed,be
R490	T880	T871	acomp	beneficial,be
R491	T881	T848	punct	.,suggested
R492	T883	T884	mark	Although,halted
R493	T884	T888	advcl	halted,found
R494	T885	T886	det	the,trials
R495	T886	T884	nsubjpass	trials,halted
R496	T887	T884	auxpass	were,halted
R497	T889	T884	prep	because,halted
R498	T890	T889	pcomp	of,because
R499	T891	T892	amod	adverse,effects
R500	T892	T889	pobj	effects,because
R501	T893	T892	prep	in,effects
R502	T894	T895	det	a,subset
R503	T895	T893	pobj	subset,in
R504	T896	T895	prep	of,subset
R505	T897	T896	pobj	volunteers,of
R506	T898	T899	punct	[,5
R507	T899	T884	parataxis	5,halted
R508	T900	T899	nummod	4,5
R509	T901	T899	punct	",",5
R510	T902	T899	punct	],5
R511	T903	T888	punct	", ",found
R512	T904	T905	amod	further,analysis
R513	T905	T888	nsubj	analysis,found
R514	T906	T905	prep	of,analysis
R515	T907	T908	amod	several,patients
R516	T908	T906	pobj	patients,of
R517	T909	T888	dobj	evidence,found
R518	T910	T911	mark	that,improved
R519	T911	T909	acl	improved,evidence
R520	T912	T913	compound	amyloid,pathology
R521	T913	T911	nsubjpass	pathology,improved
R522	T914	T913	punct	", ",pathology
R523	T915	T913	cc	and,pathology
R524	T916	T917	prep	to,function
R525	T917	T913	conj	function,pathology
R526	T918	T919	det	a,degree
R527	T919	T916	pobj	degree,to
R528	T920	T919	amod	lesser,degree
R529	T921	T917	amod	cognitive,function
R530	T922	T911	punct	", ",improved
R531	T923	T911	auxpass	was,improved
R532	T924	T911	prep	in,improved
R533	T925	T924	pobj	proportion,in
R534	T926	T925	prep	to,proportion
R535	T927	T928	det	the,patient
R536	T928	T929	poss	patient,titer
R537	T929	T926	pobj	titer,to
R538	T930	T928	case	's,patient
R539	T931	T929	prep	of,titer
R540	T932	T933	npadvmod	Aβ,specific
R541	T933	T935	amod	specific,antibody
R542	T934	T933	punct	-,specific
R543	T935	T931	pobj	antibody,of
R544	T936	T937	punct	[,7
R545	T937	T911	parataxis	7,improved
R546	T938	T937	nummod	6,7
R547	T939	T937	punct	",",7
R548	T940	T937	punct	],7
R549	T941	T888	punct	.,found
R550	T943	T944	mark	While,is
R551	T944	T947	advcl	is,have
R552	T945	T946	det	this,approach
R553	T946	T944	nsubj	approach,is
R554	T948	T944	acomp	promising,is
R555	T949	T947	punct	", ",have
R556	T950	T951	amod	constant,exposure
R557	T951	T947	nsubj	exposure,have
R558	T952	T951	prep	to,exposure
R559	T953	T952	pobj	antibodies,to
R560	T954	T955	dep	that,recognize
R561	T955	T953	relcl	recognize,antibodies
R562	T956	T957	det	an,epitope
R563	T957	T955	dobj	epitope,recognize
R564	T958	T959	advmod	highly,enriched
R565	T959	T957	amod	enriched,epitope
R566	T960	T959	prep	in,enriched
R567	T961	T962	det	the,brain
R568	T962	T960	pobj	brain,in
R569	T963	T947	aux	may,have
R570	T964	T965	amod	unexpected,effects
R571	T965	T947	dobj	effects,have
R572	T966	T965	compound	side,effects
R573	T967	T968	dep	that,limit
R574	T968	T965	relcl	limit,effects
R575	T969	T968	aux	will,limit
R576	T970	T971	poss	its,use
R577	T971	T968	dobj	use,limit
R578	T972	T973	amod	long,term
R579	T973	T971	compound	term,use
R580	T974	T973	punct	-,term
R581	T975	T947	punct	.,have
R582	T977	T978	det	An,approach
R583	T978	T980	nsubj	approach,seeks
R584	T979	T978	amod	alternative,approach
R585	T981	T982	dep	that,pursued
R586	T982	T978	relcl	pursued,approach
R587	T983	T982	aux	is,pursued
R588	T984	T982	auxpass	being,pursued
R589	T985	T982	advmod	actively,pursued
R590	T986	T982	prep	for,pursued
R591	T987	T988	amod	future,treatment
R592	T988	T986	pobj	treatment,for
R593	T989	T988	prep	of,treatment
R594	T990	T989	pobj	AD,of
R595	T991	T992	aux	to,lower
R596	T992	T980	xcomp	lower,seeks
R597	T993	T994	compound	Aβ,levels
R598	T994	T992	dobj	levels,lower
R599	T995	T992	prep	by,lower
R600	T996	T995	pcomp	limiting,by
R601	T997	T998	poss	its,production
R602	T998	T996	dobj	production,limiting
R603	T999	T996	prep	from,limiting
R604	T1000	T1001	det	the,protein
R605	T1001	T999	pobj	protein,from
R606	T1002	T1001	compound	precursor,protein
R607	T1003	T1001	appos	APP,protein
R608	T1004	T980	punct	.,seeks
R609	T1006	T1007	compound	Peptide,Aβ
R610	T1007	T1008	nsubjpass	Aβ,released
R611	T1009	T1008	auxpass	is,released
R612	T1010	T1008	prep	from,released
R613	T1011	T1010	pobj	APP,from
R614	T1012	T1008	agent	by,released
R615	T1013	T1014	det	the,action
R616	T1014	T1012	pobj	action,by
R617	T1015	T1014	prep	of,action
R618	T1016	T1017	nummod	two,enzymes
R619	T1017	T1015	pobj	enzymes,of
R620	T1018	T1017	punct	", ",enzymes
R621	T1019	T1020	det	the,enzyme
R623	T1021	T1022	compound	β,APP
R624	T1022	T1024	npadvmod	APP,cleaving
R625	T1023	T1022	punct	-,APP
R626	T1024	T1020	amod	cleaving,enzyme
R627	T1025	T1020	nummod	1,enzyme
R628	T1026	T1020	punct	(,enzyme
R629	T1027	T1020	appos	BACE1,enzyme
R630	T1028	T1020	punct	),enzyme
R631	T1029	T1020	cc	and,enzyme
R632	T1030	T1031	punct	γ,secretase
R633	T1031	T1020	conj	secretase,enzyme
R634	T1032	T1031	punct	-,secretase
R635	T1033	T1017	punct	", ",enzymes
R636	T1034	T1035	dep	which,cleave
R637	T1035	T1017	relcl	cleave,enzymes
R638	T1036	T1037	det	the,holoprotein
R639	T1037	T1035	dobj	holoprotein,cleave
R640	T1038	T1035	prep	at,cleave
R641	T1039	T1040	det	the,termini
R642	T1040	T1038	pobj	termini,at
R643	T1041	T1040	nmod	N,termini
R644	T1042	T1041	punct	-,N
R645	T1043	T1041	cc	and,N
R646	T1044	T1041	conj	C,N
R647	T1045	T1040	punct	-,termini
R648	T1046	T1040	prep	of,termini
R649	T1047	T1046	pobj	Aβ,of
R650	T1048	T1008	punct	", ",released
R651	T1049	T1008	advmod	respectively,released
R652	T1050	T1008	punct	.,released
R653	T1052	T1053	amod	Several,inhibitors
R654	T1053	T1054	nsubjpass	inhibitors,produced
R655	T1055	T1053	prep	of,inhibitors
R656	T1056	T1057	punct	γ,secretase
R657	T1057	T1055	pobj	secretase,of
R658	T1058	T1057	punct	-,secretase
R659	T1059	T1054	aux	have,produced
R660	T1060	T1054	advmod	already,produced
R661	T1061	T1054	auxpass	been,produced
R662	T1062	T1063	punct	[,9
R663	T1063	T1054	parataxis	9,produced
R664	T1064	T1063	nummod	8,9
R665	T1065	T1063	punct	",",9
R666	T1066	T1063	punct	],9
R667	T1067	T1054	punct	", ",produced
R668	T1068	T1054	cc	and,produced
R669	T1069	T1070	amod	small,inhibitors
R670	T1070	T1072	nsubjpass	inhibitors,developed
R671	T1071	T1070	compound	molecule,inhibitors
R672	T1072	T1054	conj	developed,produced
R673	T1073	T1070	prep	of,inhibitors
R674	T1074	T1075	compound	β,APP
R675	T1075	T1077	npadvmod	APP,cleaving
R676	T1076	T1075	punct	-,APP
R677	T1077	T1078	amod	cleaving,enzyme
R678	T1078	T1073	pobj	enzyme,of
R679	T1079	T1078	nummod	1,enzyme
R680	T1080	T1072	aux	are,developed
R681	T1081	T1072	advmod	currently,developed
R682	T1082	T1072	auxpass	being,developed
R683	T1083	T1084	punct	[,11
R684	T1084	T1072	parataxis	11,developed
R685	T1085	T1084	nummod	10,11
R686	T1086	T1084	punct	",",11
R687	T1087	T1084	punct	],11
R688	T1088	T1072	punct	.,developed
R689	T1090	T1091	det	The,effectiveness
R690	T1091	T1095	nsubjpass	effectiveness,reported
R691	T1092	T1093	amod	long,term
R692	T1093	T1091	compound	term,effectiveness
R693	T1094	T1093	punct	-,term
R694	T1096	T1091	prep	of,effectiveness
R695	T1097	T1098	det	this,approach
R696	T1098	T1096	pobj	approach,of
R697	T1099	T1091	prep	in,effectiveness
R698	T1100	T1101	preconj	either,humans
R699	T1101	T1099	pobj	humans,in
R700	T1102	T1101	cc	or,humans
R701	T1103	T1104	compound	model,systems
R702	T1104	T1101	conj	systems,humans
R703	T1105	T1095	punct	", ",reported
R704	T1106	T1095	advmod	however,reported
R705	T1107	T1095	punct	", ",reported
R706	T1108	T1095	aux	has,reported
R707	T1109	T1095	neg	not,reported
R708	T1110	T1095	auxpass	been,reported
R709	T1111	T1095	punct	.,reported
R710	T1113	T1114	mark	Although,prevent
R711	T1114	T1125	advcl	prevent,known
R712	T1115	T1114	nsubj	loss,prevent
R713	T1116	T1115	prep	of,loss
R714	T1117	T1118	compound	β,APP
R715	T1118	T1120	npadvmod	APP,cleaving
R716	T1119	T1118	punct	-,APP
R717	T1120	T1121	amod	cleaving,function
R718	T1121	T1116	pobj	function,of
R719	T1122	T1121	nmod	enzyme,function
R720	T1123	T1122	nummod	1,enzyme
R721	T1124	T1114	aux	can,prevent
R722	T1126	T1127	det	the,development
R723	T1127	T1114	dobj	development,prevent
R724	T1128	T1127	prep	of,development
R725	T1129	T1128	pobj	plaques,of
R726	T1130	T1114	prep	in,prevent
R727	T1131	T1132	amod	transgenic,models
R728	T1132	T1130	pobj	models,in
R729	T1133	T1132	compound	mouse,models
R730	T1134	T1132	prep	for,models
R731	T1135	T1134	pobj	AD,for
R732	T1136	T1137	punct	(,F.
R733	T1137	T1114	meta	F.,prevent
R734	T1138	T1137	nmod	Laird,F.
R735	T1139	T1137	punct	", ",F.
R736	T1140	T1137	nmod	H.,F.
R737	T1141	T1137	nmod	Cai,F.
R738	T1142	T1137	punct	", ",F.
R739	T1143	T1137	nmod	P.,F.
R740	T1144	T1137	nmod	C.,F.
R741	T1145	T1137	nmod	Wong,F.
R742	T1146	T1137	punct	", ",F.
R743	T1147	T1137	amod	personal,F.
R744	T1148	T1137	nmod	communication,F.
R745	T1149	T1137	punct	),F.
R746	T1150	T1125	punct	", ",known
R747	T1151	T1125	nsubjpass	it,known
R748	T1152	T1125	auxpass	is,known
R749	T1153	T1125	neg	not,known
R750	T1154	T1155	mark	whether,clear
R751	T1155	T1125	ccomp	clear,known
R752	T1156	T1157	det	the,brain
R753	T1157	T1155	nsubj	brain,clear
R754	T1158	T1155	aux	can,clear
R755	T1159	T1160	amod	pre-existing,deposits
R756	T1160	T1155	dobj	deposits,clear
R757	T1161	T1160	compound	amyloid,deposits
R758	T1162	T1163	mark	once,suppressed
R759	T1163	T1155	advcl	suppressed,clear
R760	T1164	T1163	nsubjpass	production,suppressed
R761	T1165	T1164	prep	of,production
R762	T1166	T1165	pobj	Aβ,of
R763	T1167	T1163	aux	has,suppressed
R764	T1168	T1163	auxpass	been,suppressed
R765	T1169	T1125	punct	.,known
R766	T1171	T1172	advmod	Clearly,examined
R767	T1173	T1172	punct	", ",examined
R768	T1174	T1175	det	the,approach
R769	T1175	T1172	nsubjpass	approach,examined
R770	T1176	T1177	npadvmod	amyloid,lowering
R771	T1177	T1175	amod	lowering,approach
R772	T1178	T1177	punct	-,lowering
R773	T1179	T1172	aux	should,examined
R774	T1180	T1172	auxpass	be,examined
R775	T1181	T1172	advmod	rigorously,examined
R776	T1182	T1172	prep	in,examined
R777	T1183	T1184	compound	animal,models
R778	T1184	T1182	pobj	models,in
R779	T1185	T1186	mark	before,tested
R780	T1186	T1172	advcl	tested,examined
R781	T1187	T1188	det	these,reagents
R782	T1188	T1186	nsubjpass	reagents,tested
R783	T1189	T1186	auxpass	are,tested
R784	T1190	T1186	prep	in,tested
R785	T1191	T1190	pobj	patients,in
R786	T1192	T1172	punct	.,examined
R787	T1194	T1195	advmod	However,are
R788	T1196	T1195	punct	", ",are
R789	T1197	T1198	det	the,inhibitors
R790	T1198	T1195	nsubj	inhibitors,are
R791	T1199	T1198	amod	chemical,inhibitors
R792	T1200	T1198	compound	secretase,inhibitors
R793	T1201	T1202	advmod	most,likely
R794	T1202	T1198	amod	likely,inhibitors
R795	T1203	T1204	aux	to,reach
R796	T1204	T1202	xcomp	reach,likely
R797	T1205	T1206	amod	human,trials
R798	T1206	T1204	dobj	trials,reach
R799	T1207	T1195	advmod	still,are
R800	T1208	T1195	prep	in,are
R801	T1209	T1208	pobj	development,in
R802	T1210	T1195	punct	.,are
R803	T1212	T1213	advmod	Therefore,developed
R804	T1214	T1213	punct	", ",developed
R805	T1215	T1213	nsubj	we,developed
R806	T1216	T1217	det	a,model
R807	T1217	T1213	dobj	model,developed
R808	T1218	T1217	compound	mouse,model
R809	T1219	T1217	prep	of,model
R810	T1220	T1221	compound	Alzheimer,type
R811	T1221	T1223	compound	type,amyloid
R812	T1222	T1221	punct	-,type
R813	T1223	T1219	pobj	amyloid,of
R814	T1224	T1225	dep	that,expresses
R815	T1225	T1223	relcl	expresses,amyloid
R816	T1226	T1227	det	a,transgene
R817	T1227	T1225	dobj	transgene,expresses
R818	T1228	T1227	amod	controllable,transgene
R819	T1229	T1227	compound	APP,transgene
R820	T1230	T1213	punct	.,developed
R821	T1232	T1233	det	This,system
R822	T1233	T1234	nsubjpass	system,regulated
R823	T1235	T1233	punct	", ",system
R824	T1236	T1237	advmod	commonly,known
R825	T1237	T1233	acl	known,system
R826	T1238	T1237	prep	as,known
R827	T1239	T1240	det	the,system
R828	T1240	T1238	pobj	system,as
R829	T1241	T1242	compound	tet,off
R830	T1242	T1240	compound	off,system
R831	T1243	T1242	punct	-,off
R832	T1244	T1234	punct	", ",regulated
R833	T1245	T1234	aux	can,regulated
R834	T1246	T1234	auxpass	be,regulated
R835	T1247	T1234	agent	by,regulated
R836	T1248	T1247	pobj	analogs,by
R837	T1249	T1248	prep	of,analogs
R838	T1250	T1249	pobj	tetracycline,of
R839	T1251	T1250	acl	administered,tetracycline
R840	T1252	T1251	prep	in,administered
R841	T1253	T1252	pobj	food,in
R842	T1254	T1253	cc	or,food
R843	T1255	T1253	conj	water,food
R844	T1256	T1257	punct	[,13
R845	T1257	T1234	parataxis	13,regulated
R846	T1258	T1257	nummod	12,13
R847	T1259	T1257	punct	",",13
R848	T1260	T1257	punct	],13
R849	T1261	T1234	punct	.,regulated
R850	T1263	T1264	det	The,levels
R851	T1264	T1267	nsubj	levels,allowed
R852	T1265	T1264	amod	strong,levels
R853	T1266	T1264	compound	expression,levels
R854	T1268	T1264	acl	produced,levels
R855	T1269	T1268	prep	with,produced
R856	T1270	T1271	det	the,vectors
R857	T1271	T1269	pobj	vectors,with
R858	T1272	T1273	compound	tet,off
R859	T1273	T1271	compound	off,vectors
R860	T1274	T1273	punct	-,off
R861	T1275	T1271	punct	", ",vectors
R862	T1276	T1271	acl	combined,vectors
R863	T1277	T1276	prep	with,combined
R864	T1278	T1279	det	the,ability
R865	T1279	T1277	pobj	ability,with
R866	T1280	T1281	aux	to,reduce
R867	T1281	T1279	acl	reduce,ability
R868	T1282	T1283	det	this,expression
R869	T1283	T1281	dobj	expression,reduce
R870	T1284	T1281	prep	by,reduce
R871	T1285	T1286	amod	several,orders
R872	T1286	T1284	pobj	orders,by
R873	T1287	T1286	prep	of,orders
R874	T1288	T1287	pobj	magnitude,of
R875	T1289	T1281	prep	with,reduce
R876	T1290	T1289	pobj	tetracycline,with
R877	T1291	T1292	punct	[,14
R878	T1292	T1281	parataxis	14,reduce
R879	T1293	T1292	punct	],14
R880	T1294	T1267	punct	", ",allowed
R881	T1295	T1267	prep	for,allowed
R882	T1296	T1297	det	a,test
R883	T1297	T1295	pobj	test,for
R884	T1298	T1297	amod	stringent,test
R885	T1299	T1297	prep	of,test
R886	T1300	T1301	advmod	how,impact
R887	T1301	T1299	pobj	impact,of
R888	T1302	T1303	det	a,inhibitor
R889	T1303	T1301	nsubj	inhibitor,impact
R890	T1304	T1305	advmod	highly,effective
R891	T1305	T1303	amod	effective,inhibitor
R892	T1306	T1303	amod	pharmaceutical,inhibitor
R893	T1307	T1303	prep	of,inhibitor
R894	T1308	T1309	compound	Aβ,production
R895	T1309	T1307	pobj	production,of
R896	T1310	T1301	aux	would,impact
R897	T1311	T1312	det	the,progression
R898	T1312	T1301	dobj	progression,impact
R899	T1313	T1312	prep	of,progression
R900	T1314	T1315	compound	amyloid,pathology
R901	T1315	T1313	pobj	pathology,of
R902	T1316	T1301	cc	and,impact
R903	T1317	T1318	mark	whether,be
R904	T1318	T1301	conj	be,impact
R905	T1319	T1318	nsubj	reversal,be
R906	T1320	T1319	prep	of,reversal
R907	T1321	T1322	det	these,lesions
R908	T1322	T1320	pobj	lesions,of
R909	T1323	T1318	aux	might,be
R910	T1324	T1318	acomp	possible,be
R911	T1325	T1318	prep	following,be
R912	T1326	T1325	prep	such,following
R913	T1327	T1326	pobj	treatment,such
R914	T1328	T1267	punct	.,allowed
R915	T1535	T1536	compound	Transgene,Construction
R916	T1538	T1539	nsubj	We,created
R917	T1540	T1541	det	a,vector
R918	T1541	T1539	dobj	vector,created
R919	T1542	T1543	npadvmod	tetracycline,responsive
R920	T1543	T1541	amod	responsive,vector
R921	T1544	T1543	punct	-,responsive
R922	T1545	T1541	amod	chimeric,vector
R923	T1546	T1541	nmod	mouse,vector
R924	T1547	T1546	punct	/,mouse
R925	T1548	T1546	amod	human,mouse
R926	T1549	T1550	nmod	APP695Swedish,Indiana
R927	T1550	T1541	nmod	Indiana,vector
R928	T1551	T1550	punct	/,Indiana
R929	T1552	T1550	punct	(,Indiana
R930	T1553	T1554	compound	swe,ind
R931	T1554	T1550	appos	ind,Indiana
R932	T1555	T1554	punct	/,ind
R933	T1556	T1541	punct	),vector
R934	T1557	T1539	prep	by,created
R935	T1558	T1557	pcomp	replacing,by
R936	T1559	T1560	det	the,region
R937	T1560	T1558	dobj	region,replacing
R938	T1561	T1560	compound	promoter,region
R939	T1562	T1560	prep	of,region
R940	T1563	T1564	det	the,vector
R941	T1564	T1562	pobj	vector,of
R942	T1565	T1564	compound	moPrP.XhoI,vector
R943	T1566	T1567	punct	(,known
R944	T1567	T1558	parataxis	known,replacing
R945	T1568	T1567	advmod	also,known
R946	T1569	T1567	prep	as,known
R947	T1570	T1571	nmod	pPrPpE1,3sal
R948	T1571	T1569	pobj	3sal,as
R949	T1572	T1571	punct	/,3sal
R950	T1573	T1571	nmod	E2,3sal
R951	T1574	T1571	punct	",",3sal
R952	T1575	T1576	punct	[,15
R953	T1576	T1567	parataxis	15,known
R954	T1577	T1576	punct	],15
R955	T1578	T1567	punct	),known
R956	T1579	T1558	prep	with,replacing
R957	T1580	T1581	det	the,promoter
R958	T1581	T1579	pobj	promoter,with
R959	T1582	T1583	npadvmod	tetracycline,responsive
R960	T1583	T1581	amod	responsive,promoter
R961	T1584	T1583	punct	-,responsive
R962	T1585	T1581	prep	of,promoter
R963	T1586	T1585	pobj	pTetSplice,of
R964	T1587	T1588	punct	(,Technologies
R965	T1588	T1586	parataxis	Technologies,pTetSplice
R966	T1589	T1588	compound	Life,Technologies
R967	T1590	T1588	punct	", ",Technologies
R968	T1591	T1588	npadvmod	Rockville,Technologies
R969	T1592	T1588	punct	", ",Technologies
R970	T1593	T1588	npadvmod	Maryland,Technologies
R971	T1594	T1588	punct	", ",Technologies
R972	T1595	T1596	compound	United,States
R973	T1596	T1588	npadvmod	States,Technologies
R974	T1597	T1588	punct	),Technologies
R975	T1598	T1558	punct	", ",replacing
R976	T1599	T1558	cc	and,replacing
R977	T1600	T1601	advmod	then,ligating
R978	T1601	T1558	conj	ligating,replacing
R979	T1602	T1603	compound	mouse,APP
R980	T1603	T1601	dobj	APP,ligating
R981	T1604	T1601	prep	with,ligating
R982	T1605	T1606	det	a,domain
R983	T1606	T1609	nmod	domain,cDNA
R984	T1607	T1606	amod	humanized,domain
R985	T1608	T1606	nmod	Aβ,domain
R986	T1609	T1604	pobj	cDNA,with
R987	T1610	T1606	punct	(,domain
R988	T1611	T1612	compound	mo,huAPP695
R989	T1612	T1606	appos	huAPP695,domain
R990	T1613	T1612	punct	/,huAPP695
R991	T1614	T1609	punct	),cDNA
R992	T1615	T1601	prep	into,ligating
R993	T1616	T1617	det	the,vector
R994	T1617	T1615	pobj	vector,into
R995	T1618	T1617	amod	new,vector
R996	T1619	T1539	punct	.,created
R997	T1621	T1622	nsubj	We,began
R998	T1623	T1622	prep	by,began
R999	T1624	T1623	pcomp	cloning,by
R1000	T1625	T1626	det	the,promoter
R1001	T1626	T1624	dobj	promoter,cloning
R1002	T1627	T1628	npadvmod	tetracycline,responsive
R1003	T1628	T1626	amod	responsive,promoter
R1004	T1629	T1628	punct	-,responsive
R1005	T1630	T1631	punct	(,6
R1006	T1631	T1626	parataxis	6,promoter
R1007	T1632	T1631	nmod	bp,6
R1008	T1633	T1634	punct	–,481
R1009	T1634	T1631	prep	481,6
R1010	T1635	T1631	punct	),6
R1011	T1636	T1624	prep	from,cloning
R1012	T1637	T1636	pobj	pTetSplice,from
R1013	T1638	T1637	prep	by,pTetSplice
R1014	T1639	T1638	pobj	PCR,by
R1015	T1640	T1639	acl	using,PCR
R1016	T1641	T1640	dobj	primers,using
R1017	T1642	T1643	dep	that,added
R1018	T1643	T1641	relcl	added,primers
R1019	T1644	T1645	amod	external,sites
R1020	T1645	T1643	dobj	sites,added
R1021	T1646	T1645	nmod	BamHI,sites
R1022	T1647	T1646	cc	and,BamHI
R1023	T1648	T1646	conj	NotI,BamHI
R1024	T1649	T1643	prep	to,added
R1025	T1650	T1651	det	the,end
R1026	T1651	T1649	pobj	end,to
R1027	T1652	T1651	nummod	5,end
R1028	T1653	T1652	punct	′,5
R1029	T1654	T1643	cc	and,added
R1030	T1655	T1656	det	a,site
R1031	T1656	T1643	conj	site,added
R1032	T1657	T1656	compound	BamHI,site
R1033	T1658	T1656	prep	to,site
R1034	T1659	T1660	det	the,end
R1035	T1660	T1658	pobj	end,to
R1036	T1661	T1660	nummod	3,end
R1037	T1662	T1661	punct	′,3
R1038	T1663	T1622	punct	", ",began
R1039	T1664	T1665	mark	while,destroying
R1040	T1665	T1622	advcl	destroying,began
R1041	T1666	T1667	nmod	XhoI,sites
R1042	T1667	T1665	dobj	sites,destroying
R1043	T1668	T1666	cc	and,XhoI
R1044	T1669	T1666	conj	BamHI,XhoI
R1045	T1670	T1667	prep	within,sites
R1046	T1671	T1672	det	the,promoter
R1047	T1672	T1670	pobj	promoter,within
R1048	T1673	T1674	punct	(,CCG
R1049	T1674	T1665	parataxis	CCG,destroying
R1050	T1675	T1676	dep	forward,C
R1051	T1676	T1674	dep	C,CCG
R1052	T1677	T1676	punct	: ,C
R1053	T1678	T1676	compound	GCC,C
R1054	T1679	T1676	compound	GGA,C
R1055	T1680	T1676	compound	TCC,C
R1056	T1681	T1676	compound	GCG,C
R1057	T1682	T1676	compound	GCC,C
R1058	T1683	T1676	compound	GCC,C
R1059	T1684	T1676	compound	GTC,C
R1060	T1685	T1676	compound	GAG,C
R1061	T1686	T1676	compound	TTT,C
R1062	T1687	T1676	compound	ACC,C
R1063	T1688	T1676	compound	ACT,C
R1064	T1689	T1676	compound	CCC,C
R1065	T1690	T1676	compound	TAT,C
R1066	T1691	T1674	punct	;,CCG
R1067	T1692	T1674	dep	reverse,CCG
R1068	T1693	T1674	punct	: ,CCG
R1069	T1694	T1674	compound	GCC,CCG
R1070	T1695	T1674	compound	GGA,CCG
R1071	T1696	T1674	compound	TCC,CCG
R1072	T1697	T1674	compound	ACT,CCG
R1073	T1698	T1674	compound	CTA,CCG
R1074	T1699	T1674	compound	GAA,CCG
R1075	T1700	T1674	compound	GAT,CCG
R1076	T1701	T1674	compound	CCC,CCG
R1077	T1702	T1674	compound	CGG,CCG
R1078	T1703	T1674	compound	GTA,CCG
R1079	T1704	T1674	punct	),CCG
R1080	T1705	T1622	punct	.,began
R1081	T1707	T1708	nsubj	We,isolated
R1082	T1709	T1708	advmod	then,isolated
R1083	T1710	T1711	det	the,intron
R1084	T1711	T1708	dobj	intron,isolated
R1085	T1712	T1711	compound	moPrP.XhoI,intron
R1086	T1713	T1708	prep	by,isolated
R1087	T1714	T1713	pobj	amplification,by
R1088	T1715	T1714	prep	with,amplification
R1089	T1716	T1715	pobj	primers,with
R1090	T1717	T1718	dep	that,added
R1091	T1718	T1716	relcl	added,primers
R1092	T1719	T1720	det	an,site
R1093	T1720	T1718	dobj	site,added
R1094	T1721	T1720	amod	external,site
R1095	T1722	T1720	compound	BamHI,site
R1096	T1723	T1718	prep	to,added
R1097	T1724	T1725	det	the,end
R1098	T1725	T1723	pobj	end,to
R1099	T1726	T1725	nummod	5,end
R1100	T1727	T1726	punct	′,5
R1101	T1728	T1725	prep	of,end
R1102	T1729	T1728	pobj	exon,of
R1103	T1730	T1729	nummod	1,exon
R1104	T1731	T1718	cc	and,added
R1105	T1732	T1718	conj	ran,added
R1106	T1733	T1732	prep	through,ran
R1107	T1734	T1735	det	the,site
R1108	T1735	T1733	pobj	site,through
R1109	T1736	T1735	compound	Asp718,site
R1110	T1737	T1735	prep	of,site
R1111	T1738	T1737	pobj	exon,of
R1112	T1739	T1738	nummod	2,exon
R1113	T1740	T1741	punct	(,TGC
R1114	T1741	T1735	parataxis	TGC,site
R1115	T1742	T1743	dep	forward,GG
R1116	T1743	T1741	dep	GG,TGC
R1117	T1744	T1743	punct	: ,GG
R1118	T1745	T1743	compound	GCC,GG
R1119	T1746	T1743	compound	GGA,GG
R1120	T1747	T1743	compound	TCC,GG
R1121	T1748	T1743	compound	GAT,GG
R1122	T1749	T1743	compound	CAG,GG
R1123	T1750	T1743	compound	CAG,GG
R1124	T1751	T1743	compound	ACC,GG
R1125	T1752	T1743	compound	GAT,GG
R1126	T1753	T1743	compound	TCT,GG
R1127	T1754	T1741	punct	;,TGC
R1128	T1755	T1741	dep	reverse,TGC
R1129	T1756	T1741	punct	: ,TGC
R1130	T1757	T1741	compound	GCC,TGC
R1131	T1758	T1741	compound	GGT,TGC
R1132	T1759	T1741	compound	ACC,TGC
R1133	T1760	T1741	compound	ACT,TGC
R1134	T1761	T1741	compound	AGG,TGC
R1135	T1762	T1741	compound	AAG,TGC
R1136	T1763	T1741	compound	GCA,TGC
R1137	T1764	T1741	compound	GAA,TGC
R1138	T1765	T1741	punct	),TGC
R1139	T1766	T1708	punct	.,isolated
R1140	T1768	T1769	det	This,fragment
R1142	T1770	T1771	nummod	2,kb
R1143	T1771	T1769	compound	kb,fragment
R1144	T1772	T1771	punct	-,kb
R1145	T1773	T1769	compound	intron,fragment
R1146	T1775	T1774	aux	was,cloned
R1147	T1776	T1774	prep	into,cloned
R1148	T1777	T1778	det	a,vector
R1149	T1778	T1776	pobj	vector,into
R1150	T1779	T1780	compound	TA,cloning
R1151	T1780	T1778	compound	cloning,vector
R1152	T1781	T1782	punct	(,Invitrogen
R1153	T1782	T1778	parataxis	Invitrogen,vector
R1154	T1783	T1782	punct	", ",Invitrogen
R1155	T1784	T1782	npadvmod	Carlsbad,Invitrogen
R1156	T1785	T1782	punct	", ",Invitrogen
R1157	T1786	T1782	npadvmod	California,Invitrogen
R1158	T1787	T1782	punct	", ",Invitrogen
R1159	T1788	T1789	compound	United,States
R1160	T1789	T1782	npadvmod	States,Invitrogen
R1161	T1790	T1782	punct	),Invitrogen
R1162	T1791	T1774	punct	", ",cloned
R1163	T1792	T1793	advmod	then,excised
R1164	T1793	T1774	conj	excised,cloned
R1165	T1794	T1793	prep	by,excised
R1166	T1795	T1796	compound	Asp718,digestion
R1167	T1796	T1794	pobj	digestion,by
R1168	T1797	T1793	cc	and,excised
R1169	T1798	T1793	conj	ligated,excised
R1170	T1799	T1798	prep	to,ligated
R1171	T1800	T1801	det	the,fragment
R1173	T1802	T1803	nummod	6.8,kb
R1174	T1803	T1801	compound	kb,fragment
R1175	T1804	T1803	punct	-,kb
R1176	T1805	T1801	compound	Asp718,fragment
R1177	T1806	T1801	prep	of,fragment
R1178	T1807	T1806	pobj	moPrP.XhoI,of
R1179	T1808	T1801	acl	containing,fragment
R1180	T1809	T1808	dobj	exon,containing
R1181	T1810	T1809	nummod	2,exon
R1182	T1811	T1809	punct	", ",exon
R1183	T1812	T1809	conj	exon,exon
R1184	T1813	T1812	nummod	3,exon
R1185	T1814	T1812	punct	", ",exon
R1186	T1815	T1816	det	the,UTR
R1187	T1816	T1812	conj	UTR,exon
R1188	T1817	T1816	nummod	3,UTR
R1189	T1818	T1817	punct	′,3
R1190	T1819	T1816	punct	", ",UTR
R1191	T1820	T1816	cc	and,UTR
R1192	T1821	T1816	conj	pBluescript,UTR
R1193	T1822	T1823	aux	to,generate
R1194	T1823	T1774	advcl	generate,cloned
R1195	T1824	T1825	det	an,vector
R1196	T1825	T1823	dobj	vector,generate
R1197	T1826	T1825	amod	intermediate,vector
R1198	T1827	T1823	prep	with,generate
R1199	T1828	T1829	det	all,exons
R1200	T1829	T1827	pobj	exons,with
R1201	T1830	T1829	nummod	three,exons
R1202	T1831	T1829	cc	and,exons
R1203	T1832	T1833	det	a,intron
R1204	T1833	T1829	conj	intron,exons
R1205	T1834	T1833	amod	central,intron
R1206	T1835	T1829	cc	but,exons
R1207	T1836	T1837	det	no,promoter
R1208	T1837	T1829	conj	promoter,exons
R1209	T1838	T1774	punct	.,cloned
R1210	T1840	T1841	det	This,vector
R1211	T1841	T1842	nsubjpass	vector,opened
R1212	T1843	T1842	auxpass	was,opened
R1213	T1844	T1842	advmod	then,opened
R1214	T1845	T1842	prep	at,opened
R1215	T1846	T1847	det	the,site
R1216	T1847	T1845	pobj	site,at
R1217	T1848	T1847	compound	BamHI,site
R1218	T1849	T1847	acl	introduced,site
R1219	T1850	T1849	agent	by,introduced
R1220	T1851	T1852	det	the,primer
R1221	T1852	T1850	pobj	primer,by
R1222	T1853	T1852	compound	intron,primer
R1223	T1854	T1852	compound	cloning,primer
R1224	T1855	T1842	punct	", ",opened
R1225	T1856	T1842	cc	and,opened
R1226	T1857	T1842	conj	ligated,opened
R1227	T1858	T1857	prep	to,ligated
R1228	T1859	T1860	det	the,fragment
R1229	T1860	T1858	pobj	fragment,to
R1230	T1861	T1862	nummod	0.5,kb
R1231	T1862	T1860	compound	kb,fragment
R1232	T1863	T1862	punct	-,kb
R1233	T1864	T1865	compound	BamHI,cut
R1234	T1865	T1860	compound	cut,fragment
R1235	T1866	T1865	punct	-,cut
R1236	T1867	T1868	compound	tetracycline,promoter
R1237	T1868	T1860	compound	promoter,fragment
R1238	T1869	T1842	punct	.,opened
R1239	T1871	T1872	det	This,ligation
R1240	T1872	T1873	nsubj	ligation,generated
R1241	T1874	T1875	det	a,vector
R1242	T1875	T1873	dobj	vector,generated
R1243	T1876	T1877	nummod	9.3,kb
R1244	T1877	T1875	compound	kb,vector
R1245	T1878	T1877	punct	-,kb
R1246	T1879	T1875	acl	encoding,vector
R1247	T1880	T1881	det	the,promoter
R1248	T1881	T1879	dobj	promoter,encoding
R1249	T1882	T1881	compound	tetracycline,promoter
R1250	T1883	T1881	prep	from,promoter
R1251	T1884	T1883	pobj	pTetSplice,from
R1252	T1885	T1879	prep	with,encoding
R1253	T1886	T1887	nummod	two,exons
R1254	T1887	T1885	pobj	exons,with
R1255	T1888	T1887	punct	", ",exons
R1256	T1889	T1890	nummod	one,intron
R1257	T1890	T1887	conj	intron,exons
R1258	T1891	T1890	punct	", ",intron
R1259	T1892	T1890	cc	and,intron
R1260	T1893	T1894	det	the,UTR
R1261	T1894	T1890	conj	UTR,intron
R1262	T1895	T1894	amod	original,UTR
R1263	T1896	T1894	nummod	3,UTR
R1264	T1897	T1896	punct	′,3
R1265	T1898	T1894	prep	of,UTR
R1266	T1899	T1900	det	the,vector
R1267	T1900	T1898	pobj	vector,of
R1268	T1901	T1900	compound	moPrP.XhoI,vector
R1269	T1902	T1887	punct	", ",exons
R1270	T1903	T1904	advmod	all,carried
R1271	T1904	T1887	acl	carried,exons
R1272	T1905	T1904	prep	in,carried
R1273	T1906	T1907	det	the,vector
R1274	T1907	T1905	pobj	vector,in
R1275	T1908	T1909	compound	pBluescript,cloning
R1276	T1909	T1907	compound	cloning,vector
R1277	T1910	T1873	punct	.,generated
R1278	T1912	T1913	nsubj	We,incorporated
R1279	T1913	T1914	ccomp	incorporated,generated
R1280	T1915	T1916	det	the,mutations
R1281	T1916	T1913	dobj	mutations,incorporated
R1282	T1917	T1916	amod	Swedish,mutations
R1283	T1918	T1919	punct	(,571NL
R1284	T1919	T1917	parataxis	571NL,Swedish
R1285	T1920	T1919	compound	KM570,571NL
R1286	T1921	T1919	punct	/,571NL
R1287	T1922	T1919	punct	),571NL
R1288	T1923	T1917	cc	and,Swedish
R1289	T1924	T1917	conj	Indiana,Swedish
R1290	T1925	T1926	punct	(,V617F
R1291	T1926	T1924	parataxis	V617F,Indiana
R1292	T1927	T1926	punct	),V617F
R1293	T1928	T1913	prep	into,incorporated
R1294	T1929	T1930	det	the,cDNA
R1295	T1930	T1928	pobj	cDNA,into
R1296	T1931	T1932	compound	mo,huAPP695
R1297	T1932	T1930	compound	huAPP695,cDNA
R1298	T1933	T1932	punct	/,huAPP695
R1299	T1934	T1930	punct	(,cDNA
R1300	T1935	T1930	prep	in,cDNA
R1301	T1936	T1937	compound	BS,KS
R1302	T1937	T1935	pobj	KS,in
R1303	T1938	T1937	punct	-,KS
R1304	T1939	T1913	punct	),incorporated
R1305	T1940	T1913	prep	by,incorporated
R1306	T1941	T1940	pobj	PCR,by
R1307	T1942	T1913	advcl	using,incorporated
R1308	T1943	T1944	det	a,strategy
R1309	T1944	T1942	dobj	strategy,using
R1310	T1945	T1946	nummod	four,primer
R1311	T1946	T1944	compound	primer,strategy
R1312	T1947	T1946	punct	-,primer
R1313	T1948	T1914	punct	: ,generated
R1314	T1949	T1914	advmod	first,generated
R1315	T1950	T1914	punct	", ",generated
R1316	T1951	T1952	nummod	two,products
R1317	T1952	T1914	nsubjpass	products,generated
R1318	T1953	T1954	advmod	partially,overlapping
R1319	T1954	T1952	amod	overlapping,products
R1320	T1955	T1914	auxpass	were,generated
R1321	T1956	T1914	prep	in,generated
R1322	T1957	T1958	amod	separate,reactions
R1323	T1958	T1956	pobj	reactions,in
R1324	T1959	T1914	advcl	using,generated
R1325	T1960	T1959	dobj	primers,using
R1326	T1961	T1962	dep	that,encode
R1327	T1962	T1960	relcl	encode,primers
R1328	T1963	T1964	det	the,mutations
R1329	T1964	T1962	dobj	mutations,encode
R1330	T1965	T1964	amod	desired,mutations
R1331	T1966	T1967	punct	(,M13
R1332	T1967	T1962	parataxis	M13,encode
R1333	T1968	T1969	compound	Swedish,forward
R1334	T1969	T1970	dep	forward,CCG
R1335	T1970	T1983	dep	CCG,C
R1336	T1971	T1970	punct	: ,CCG
R1337	T1972	T1970	compound	GGA,CCG
R1338	T1973	T1970	compound	GAT,CCG
R1339	T1974	T1970	compound	CTC,CCG
R1340	T1975	T1970	compound	TGA,CCG
R1341	T1976	T1970	compound	AGT,CCG
R1342	T1977	T1970	compound	GAA,CCG
R1343	T1978	T1970	compound	TCT,CCG
R1344	T1979	T1970	compound	GGA,CCG
R1345	T1980	T1970	compound	TGC,CCG
R1346	T1981	T1970	compound	AGA,CCG
R1347	T1982	T1970	compound	ATT,CCG
R1348	T1983	T1967	dep	C,M13
R1349	T1984	T1983	punct	/,C
R1350	T1985	T1986	compound	Indiana,reverse
R1351	T1986	T1983	dep	reverse,C
R1352	T1987	T1983	punct	: ,C
R1353	T1988	T1983	compound	GGG,C
R1354	T1989	T1983	compound	TGA,C
R1355	T1990	T1983	compound	TGA,C
R1356	T1991	T1983	compound	AAA,C
R1357	T1992	T1983	compound	TCA,C
R1358	T1993	T1983	compound	CGG,C
R1359	T1994	T1983	compound	TTG,C
R1360	T1995	T1967	punct	;,M13
R1361	T1996	T1997	compound	Indiana,forward
R1362	T1997	T1967	dep	forward,M13
R1363	T1998	T1967	punct	: ,M13
R1364	T1999	T1967	compound	CAA,M13
R1365	T2000	T1967	compound	CCG,M13
R1366	T2001	T1967	compound	TGA,M13
R1367	T2002	T1967	compound	TTT,M13
R1368	T2003	T1967	compound	TCA,M13
R1369	T2004	T1967	compound	TCA,M13
R1370	T2005	T1967	compound	CCC,M13
R1371	T2006	T1967	compound	TGG,M13
R1372	T2007	T1967	punct	/,M13
R1373	T2008	T1967	advmod	reverse,M13
R1374	T2009	T1967	punct	),M13
R1375	T2010	T1914	punct	.,generated
R1376	T2012	T2013	det	The,products
R1377	T2013	T2016	nsubjpass	products,ligated
R1378	T2014	T2013	nummod	two,products
R1379	T2015	T2013	compound	PCR,products
R1380	T2017	T2016	auxpass	were,ligated
R1381	T2018	T2016	punct	", ",ligated
R1382	T2019	T2016	dep	digested,ligated
R1383	T2020	T2016	prep	with,ligated
R1384	T2021	T2020	pobj	BglII,with
R1385	T2022	T2021	cc	and,BglII
R1386	T2023	T2021	conj	ApaI,BglII
R1387	T2024	T2016	cc	and,ligated
R1388	T2025	T2016	conj	cloned,ligated
R1389	T2026	T2025	advmod	back,cloned
R1390	T2027	T2025	prep	into,cloned
R1391	T2028	T2029	det	the,vector
R1392	T2029	T2027	pobj	vector,into
R1393	T2030	T2029	amod	original,vector
R1394	T2031	T2032	compound	mo,KS
R1396	T2033	T2032	punct	/,KS
R1397	T2034	T2032	compound	huAPP695,KS
R1398	T2035	T2032	punct	-,KS
R1399	T2036	T2032	compound	BS,KS
R1400	T2037	T2032	punct	-,KS
R1401	T2038	T2016	punct	.,ligated
R1402	T2040	T2041	advmod	Finally,subcloned
R1403	T2042	T2041	punct	", ",subcloned
R1404	T2043	T2044	det	the,ind
R1405	T2044	T2041	nsubjpass	ind,subcloned
R1406	T2045	T2044	amod	new,ind
R1407	T2046	T2044	compound	APP695swe,ind
R1408	T2047	T2044	punct	/,ind
R1409	T2048	T2041	auxpass	was,subcloned
R1410	T2049	T2041	prep	into,subcloned
R1411	T2050	T2051	det	the,site
R1412	T2051	T2049	pobj	site,into
R1413	T2052	T2051	compound	XhoI,site
R1414	T2053	T2051	prep	of,site
R1415	T2054	T2055	det	the,vector
R1416	T2055	T2053	pobj	vector,of
R1417	T2056	T2057	compound	moPrP,tetP
R1418	T2057	T2055	compound	tetP,vector
R1419	T2058	T2057	punct	-,tetP
R1420	T2059	T2041	prep	from,subcloned
R1421	T2060	T2059	pcomp	above,from
R1422	T2061	T2062	aux	to,complete
R1423	T2062	T2041	advcl	complete,subcloned
R1424	T2063	T2064	det	the,construct
R1425	T2064	T2062	dobj	construct,complete
R1426	T2065	T2041	punct	.,subcloned
R3744	T5349	T5347	pobj	mice,in
R3745	T5350	T5351	nmod	CaMKIIα,ind
R3746	T5351	T5349	nmod	ind,mice
R3747	T5352	T5351	punct	-,ind
R3748	T5353	T5351	nmod	tTA,ind
R3749	T5354	T5351	punct	×,ind
R3750	T5355	T5351	nmod	tet,ind
R3751	T5356	T5351	punct	-,ind
R3752	T5357	T5351	nmod	APPswe,ind
R3753	T5358	T5351	punct	/,ind
R3754	T5359	T5349	nmod	line,mice
R3755	T5360	T5359	nummod	107,line
R3756	T5361	T5362	quantmod	between,4
R3757	T5362	T5363	nummod	4,mo
R3758	T5363	T5349	npadvmod	mo,mice
R3759	T5364	T5362	cc	and,4
R3760	T5365	T5362	conj	5,4
R3761	T5366	T5363	prep	of,mo
R3762	T5367	T5366	pobj	age,of
R3763	T5368	T5345	punct	.,studied
R3764	T5370	T5371	nsubjpass	Animals,separated
R3765	T5372	T5371	auxpass	were,separated
R3766	T5373	T5371	prep	into,separated
R3767	T5374	T5375	amod	individual,cages
R3768	T5375	T5373	pobj	cages,into
R3769	T5376	T5377	advmod	immediately,before
R3770	T5377	T5371	prep	before,separated
R3771	T5378	T5379	det	the,start
R3772	T5379	T5377	pobj	start,before
R3773	T5380	T5379	prep	of,start
R3774	T5381	T5382	det	each,experiment
R3775	T5382	T5380	pobj	experiment,of
R3776	T5383	T5384	punct	(,6
R3777	T5384	T5371	parataxis	6,separated
R3778	T5385	T5384	nsubj	n,6
R3779	T5386	T5384	punct	=,6
R3780	T5387	T5384	quantmod	3,6
R3781	T5388	T5384	punct	–,6
R3782	T5389	T5384	prep	per,6
R3783	T5390	T5389	pobj	genotype,per
R3784	T5391	T5390	amod	untreated,genotype
R3785	T5392	T5384	punct	", ",6
R3786	T5393	T5394	quantmod	2,5
R3787	T5394	T5384	appos	5,6
R3788	T5395	T5394	punct	–,5
R3789	T5396	T5394	prep	per,5
R3790	T5397	T5396	pobj	genotype,per
R3791	T5398	T5399	dep	dox,reared
R3792	T5399	T5397	acl	reared,genotype
R3793	T5400	T5399	punct	-,reared
R3794	T5401	T5384	punct	),6
R3795	T5402	T5371	punct	.,separated
R3796	T5404	T5405	det	The,cages
R3797	T5405	T5406	nsubjpass	cages,placed
R3798	T5407	T5406	auxpass	were,placed
R3799	T5408	T5406	prep	inside,placed
R3800	T5409	T5410	npadvmod	activity,monitoring
R3801	T5410	T5412	amod	monitoring,frames
R3802	T5411	T5410	punct	-,monitoring
R3803	T5412	T5408	pobj	frames,inside
R3804	T5413	T5412	acl	designed,frames
R3805	T5414	T5415	aux	to,count
R3806	T5415	T5413	advcl	count,designed
R3807	T5416	T5417	det	every,time
R3808	T5417	T5415	dobj	time,count
R3809	T5418	T5419	det	the,animal
R3810	T5419	T5420	nsubj	animal,passed
R3811	T5420	T5417	advcl	passed,time
R3812	T5421	T5420	prep	through,passed
R3813	T5422	T5421	pobj	one,through
R3814	T5423	T5422	prep	of,one
R3815	T5424	T5425	nummod	three,photobeams
R3816	T5425	T5423	pobj	photobeams,of
R3817	T5426	T5425	acl	spanning,photobeams
R3818	T5427	T5428	det	the,width
R3819	T5428	T5426	dobj	width,spanning
R3820	T5429	T5428	prep	of,width
R3821	T5430	T5431	det	the,cage
R3822	T5431	T5429	pobj	cage,of
R3823	T5432	T5433	punct	(,Instruments
R3824	T5433	T5420	parataxis	Instruments,passed
R3825	T5434	T5435	compound	San,Diego
R3826	T5435	T5433	compound	Diego,Instruments
R3827	T5436	T5433	punct	", ",Instruments
R3828	T5437	T5438	compound	San,Diego
R3829	T5438	T5433	npadvmod	Diego,Instruments
R3830	T5439	T5433	punct	", ",Instruments
R3831	T5440	T5433	npadvmod	California,Instruments
R3832	T5441	T5433	punct	", ",Instruments
R3833	T5442	T5443	compound	United,States
R3834	T5443	T5433	npadvmod	States,Instruments
R3835	T5444	T5433	punct	),Instruments
R3836	T5445	T5406	punct	.,placed
R3837	T5447	T5448	nsubjpass	Experiments,started
R3838	T5449	T5448	auxpass	were,started
R3839	T5450	T5451	advmod	midway,through
R3840	T5451	T5448	prep	through,started
R3841	T5452	T5453	det	the,phase
R3842	T5453	T5451	pobj	phase,through
R3843	T5454	T5453	compound	light,phase
R3844	T5455	T5453	prep	of,phase
R3845	T5456	T5457	det	the,day
R3846	T5457	T5455	pobj	day,of
R3847	T5458	T5448	punct	", ",started
R3848	T5459	T5448	cc	and,started
R3849	T5460	T5461	nsubjpass	data,collected
R3850	T5461	T5448	conj	collected,started
R3851	T5462	T5461	auxpass	were,collected
R3852	T5463	T5461	prep	in,collected
R3853	T5464	T5465	nummod	1,h
R3854	T5465	T5467	compound	h,bins
R3855	T5466	T5465	punct	-,h
R3856	T5467	T5463	pobj	bins,in
R3857	T5468	T5461	prep	for,collected
R3858	T5469	T5470	det	the,h
R3859	T5470	T5468	pobj	h,for
R3860	T5471	T5470	amod	following,h
R3861	T5472	T5470	nummod	48,h
R3862	T5473	T5461	punct	.,collected
R3863	T5475	T5476	compound	Testing,rooms
R3864	T5476	T5477	nsubjpass	rooms,maintained
R3865	T5478	T5477	auxpass	were,maintained
R3866	T5479	T5477	prep	on,maintained
R3867	T5480	T5481	det	the,cycle
R3868	T5481	T5479	pobj	cycle,on
R3869	T5482	T5481	amod	same,cycle
R3870	T5483	T5484	nummod	13,h
R3871	T5484	T5481	nmod	h,cycle
R3872	T5485	T5486	punct	:,11
R3873	T5486	T5483	prep	11,13
R3874	T5487	T5481	nmod	day,cycle
R3875	T5488	T5489	punct	:,night
R3876	T5489	T5487	prep	night,day
R3877	T5490	T5481	prep	as,cycle
R3878	T5491	T5492	det	the,areas
R3879	T5492	T5490	pobj	areas,as
R3880	T5493	T5492	amod	main,areas
R3881	T5494	T5495	compound	animal,housing
R3882	T5495	T5492	compound	housing,areas
R3883	T5496	T5477	cc	and,maintained
R3884	T5497	T5498	auxpass	were,closed
R3885	T5498	T5477	conj	closed,maintained
R3886	T5499	T5498	prep	to,closed
R3887	T5500	T5499	pobj	entry,to
R3888	T5501	T5498	prep	during,closed
R3889	T5502	T5503	det	the,experiment
R3890	T5503	T5501	pobj	experiment,during
R3891	T5504	T5477	punct	.,maintained
R3892	T5510	T5511	amod	Statistical,Analyses
R3893	T5513	T5514	amod	Statistical,analyses
R3894	T5514	T5515	nsubjpass	analyses,performed
R3895	T5516	T5514	prep	of,analyses
R3896	T5517	T5518	compound	protein,expression
R3897	T5518	T5516	pobj	expression,of
R3898	T5519	T5518	punct	", ",expression
R3899	T5520	T5521	compound	ELISA,data
R3900	T5521	T5518	conj	data,expression
R3901	T5522	T5521	punct	", ",data
R3902	T5523	T5521	cc	and,data
R3903	T5524	T5525	compound	filter,trap
R3904	T5525	T5526	compound	trap,assays
R3905	T5526	T5521	conj	assays,data
R3906	T5527	T5515	auxpass	were,performed
R3907	T5528	T5515	prep	by,performed
R3908	T5529	T5528	pobj	ANOVA,by
R3909	T5530	T5515	prep	with,performed
R3910	T5531	T5532	poss	Tukey,test
R3911	T5532	T5530	pobj	test,with
R3912	T5533	T5531	case	's,Tukey
R3913	T5534	T5532	amod	honest,test
R3914	T5535	T5532	amod	significant,test
R3915	T5536	T5532	nmod	difference,test
R3916	T5537	T5538	nmod	post,hoc
R3917	T5538	T5532	nmod	hoc,test
R3918	T5539	T5538	punct	-,hoc
R3919	T5540	T5532	acl	applied,test
R3920	T5541	T5540	prep	to,applied
R3921	T5542	T5543	amod	significant,effects
R3922	T5543	T5541	pobj	effects,to
R3923	T5544	T5543	amod	main,effects
R3924	T5545	T5543	cc	or,effects
R3925	T5546	T5543	conj	interactions,effects
R3926	T5547	T5548	punct	(,Statistica
R3927	T5548	T5515	parataxis	Statistica,performed
R3928	T5549	T5548	nummod	6.0,Statistica
R3929	T5550	T5548	punct	", ",Statistica
R3930	T5551	T5548	npadvmod	StatSoft,Statistica
R3931	T5552	T5548	punct	", ",Statistica
R3932	T5553	T5548	npadvmod	Tulsa,Statistica
R3933	T5554	T5548	punct	", ",Statistica
R3934	T5555	T5548	npadvmod	Oklahoma,Statistica
R3935	T5556	T5548	punct	", ",Statistica
R3936	T5557	T5558	compound	United,States
R3937	T5558	T5548	npadvmod	States,Statistica
R3938	T5559	T5548	punct	),Statistica
R3939	T5560	T5515	punct	.,performed
R3940	T5562	T5563	prep	In,applied
R3941	T5564	T5562	pobj	cases,In
R3942	T5565	T5564	prep	of,cases
R3943	T5566	T5567	advmod	positively,skewed
R3944	T5567	T5568	amod	skewed,distribution
R3945	T5568	T5565	pobj	distribution,of
R3946	T5569	T5568	compound	data,distribution
R3947	T5570	T5563	punct	", ",applied
R3948	T5571	T5572	nmod	log10,x
R3949	T5572	T5574	nmod	x,transformation
R3950	T5573	T5572	punct	(,x
R3951	T5574	T5563	nsubjpass	transformation,applied
R3952	T5575	T5572	punct	+,x
R3953	T5576	T5572	nummod	0.5,x
R3954	T5577	T5572	punct	),x
R3955	T5578	T5563	auxpass	was,applied
R3956	T5579	T5563	prep	to,applied
R3957	T5580	T5581	det	the,data
R3958	T5581	T5579	pobj	data,to
R3959	T5582	T5581	amod	raw,data
R3960	T5583	T5563	prep	before,applied
R3961	T5584	T5583	pcomp	submitting,before
R3962	T5585	T5584	dobj	them,submitting
R3963	T5586	T5584	prep	to,submitting
R3964	T5587	T5586	pobj	ANOVA,to
R3965	T5588	T5563	punct	.,applied
R3967	T6511	T6512	nsubj	We,used
R3968	T6513	T6514	det	the,system
R3969	T6514	T6512	dobj	system,used
R3970	T6515	T6516	compound	tet,off
R3971	T6516	T6514	compound	off,system
R3972	T6517	T6516	punct	-,off
R3973	T6518	T6514	compound	transgene,system
R3974	T6519	T6520	aux	to,express
R3975	T6520	T6512	advcl	express,used
R3976	T6521	T6522	det	a,version
R3977	T6522	T6520	dobj	version,express
R3978	T6523	T6522	amod	double,version
R3979	T6524	T6522	compound	mutant,version
R3980	T6525	T6522	prep	of,version
R3981	T6526	T6527	amod	chimeric,huAPP695
R3982	T6527	T6525	pobj	huAPP695,of
R3983	T6528	T6527	compound	mo,huAPP695
R3984	T6529	T6527	punct	/,huAPP695
R3985	T6530	T6522	punct	(,version
R3986	T6531	T6532	compound	swe,KM570
R3987	T6532	T6522	appos	KM570,version
R3988	T6533	T6532	punct	/,KM570
R3989	T6534	T6532	compound	ind,KM570
R3990	T6535	T6532	punct	", ",KM570
R3991	T6536	T6532	conj	571NL,KM570
R3992	T6537	T6536	punct	", ",571NL
R3993	T6538	T6536	cc	and,571NL
R3994	T6539	T6536	conj	V617F,571NL
R3995	T6540	T6520	punct	),express
R3996	T6541	T6520	prep	from,express
R3997	T6542	T6543	det	a,promoter
R3998	T6543	T6541	pobj	promoter,from
R3999	T6544	T6545	npadvmod	tetracycline,responsive
R4000	T6545	T6543	amod	responsive,promoter
R4001	T6546	T6545	punct	-,responsive
R4002	T6547	T6548	punct	[,13
R4003	T6548	T6520	parataxis	13,express
R4004	T6549	T6548	nummod	12,13
R4005	T6550	T6548	punct	",",13
R4006	T6551	T6548	punct	],13
R4007	T6552	T6512	punct	.,used
R4008	T6554	T6555	amod	Transgenic,APP
R4009	T6555	T6556	compound	APP,expression
R4010	T6556	T6557	nsubjpass	expression,activated
R4011	T6558	T6557	auxpass	was,activated
R4012	T6559	T6557	prep	by,activated
R4013	T6560	T6559	pcomp	crossing,by
R4014	T6561	T6562	det	the,mice
R4015	T6562	T6560	dobj	mice,crossing
R4016	T6563	T6564	compound	APPswe,ind
R4017	T6564	T6562	compound	ind,mice
R4018	T6565	T6564	punct	/,ind
R4019	T6566	T6560	prep	to,crossing
R4020	T6567	T6566	pobj	animals,to
R4021	T6568	T6567	acl	producing,animals
R4022	T6569	T6568	dobj	tTA,producing
R4023	T6570	T6568	prep	under,producing
R4024	T6571	T6570	pobj	control,under
R4025	T6572	T6571	prep	of,control
R4026	T6573	T6574	det	the,promoter
R4027	T6574	T6572	pobj	promoter,of
R4028	T6575	T6574	compound	CaMKIIα,promoter
R4029	T6576	T6577	punct	[,16
R4030	T6577	T6568	parataxis	16,producing
R4031	T6578	T6577	punct	],16
R4032	T6579	T6557	punct	.,activated
R4033	T6581	T6582	prep	After,identified
R4034	T6583	T6584	amod	initial,screening
R4035	T6584	T6581	pobj	screening,After
R4036	T6585	T6584	prep	of,screening
R4037	T6586	T6585	pobj	founders,of
R4038	T6587	T6582	punct	", ",identified
R4039	T6588	T6582	nsubj	we,identified
R4040	T6589	T6590	nummod	four,lines
R4041	T6590	T6582	dobj	lines,identified
R4042	T6591	T6590	prep	of,lines
R4043	T6592	T6593	compound	tet,ind
R4044	T6593	T6597	compound	ind,mice
R4045	T6594	T6593	punct	-,ind
R4046	T6595	T6593	compound	APPswe,ind
R4047	T6596	T6593	punct	/,ind
R4048	T6597	T6591	pobj	mice,of
R4049	T6598	T6599	dep	that,produced
R4050	T6599	T6590	relcl	produced,lines
R4051	T6600	T6601	advmod	very,high
R4052	T6601	T6602	amod	high,levels
R4053	T6602	T6599	dobj	levels,produced
R4054	T6603	T6602	prep	of,levels
R4055	T6604	T6605	compound	transgene,product
R4056	T6605	T6603	pobj	product,of
R4057	T6606	T6599	prep	in,produced
R4058	T6607	T6606	pobj	offspring,in
R4059	T6608	T6607	acl	coexpressing,offspring
R4060	T6609	T6608	dobj	tTA,coexpressing
R4061	T6610	T6611	punct	(,Figures
R4062	T6611	T6599	parataxis	Figures,produced
R4063	T6612	T6611	nummod	1,Figures
R4064	T6613	T6611	cc	and,Figures
R4065	T6614	T6615	compound	Figure,S1
R4066	T6615	T6611	conj	S1,Figures
R4067	T6616	T6611	punct	),Figures
R4068	T6617	T6582	punct	.,identified
R4069	T6619	T6620	prep	Compared,estimated
R4070	T6621	T6619	prep	to,Compared
R4071	T6622	T6623	det	a,line
R4072	T6623	T6621	pobj	line,to
R4073	T6624	T6623	amod	standard,line
R4074	T6625	T6623	nmod	APP,line
R4075	T6626	T6623	amod	transgenic,line
R4076	T6627	T6623	acl	used,line
R4077	T6628	T6627	prep	for,used
R4078	T6629	T6630	amod	previous,studies
R4079	T6630	T6628	pobj	studies,for
R4080	T6631	T6630	compound	amyloid,studies
R4081	T6632	T6630	prep	by,studies
R4082	T6633	T6634	poss	our,laboratory
R4083	T6634	T6632	pobj	laboratory,by
R4084	T6635	T6636	punct	(,27
R4085	T6636	T6627	parataxis	27,used
R4086	T6637	T6638	compound	line,C3
R4087	T6638	T6636	dep	C3,27
R4088	T6639	T6638	punct	–,C3
R4089	T6640	T6638	nummod	3,C3
R4090	T6641	T6636	punct	;,27
R4091	T6642	T6636	punct	[,27
R4092	T6643	T6636	nummod	15,27
R4093	T6644	T6636	punct	",",27
R4094	T6645	T6636	nummod	26,27
R4095	T6646	T6636	punct	",",27
R4096	T6647	T6636	punct	],27
R4097	T6648	T6636	punct	),27
R4098	T6649	T6620	punct	", ",estimated
R4099	T6650	T6620	nsubj	we,estimated
R4100	T6651	T6652	mark	that,produce
R4101	T6652	T6620	ccomp	produce,estimated
R4102	T6653	T6654	det	the,lines
R4103	T6654	T6652	nsubj	lines,produce
R4104	T6655	T6654	nummod	four,lines
R4105	T6656	T6654	amod	controllable,lines
R4106	T6657	T6658	amod	transgenic,protein
R4107	T6658	T6652	dobj	protein,produce
R4108	T6659	T6658	compound	APP,protein
R4109	T6660	T6652	prep	at,produce
R4110	T6661	T6660	pcomp	10,at
R4111	T6662	T6661	punct	-,10
R4112	T6663	T6661	quantmod	to,10
R4113	T6664	T6661	advmod	30-fold,10
R4114	T6665	T6661	prep	over,10
R4115	T6666	T6667	amod	endogenous,levels
R4116	T6667	T6665	pobj	levels,over
R4117	T6668	T6669	punct	(,S1
R4118	T6669	T6652	parataxis	S1,produce
R4119	T6670	T6669	compound	Figure,S1
R4120	T6671	T6669	punct	),S1
R4121	T6672	T6620	punct	.,estimated
R4122	T6674	T6675	det	This,estimate
R4123	T6675	T6676	nsubjpass	estimate,confirmed
R4124	T6677	T6676	auxpass	was,confirmed
R4125	T6678	T6676	agent	by,confirmed
R4126	T6679	T6680	amod	direct,comparison
R4127	T6680	T6678	pobj	comparison,by
R4128	T6681	T6680	prep	of,comparison
R4129	T6682	T6683	compound	APP,levels
R4130	T6683	T6681	pobj	levels,of
R4131	T6684	T6680	prep	in,comparison
R4132	T6685	T6686	amod	nontransgenic,mice
R4133	T6686	T6684	pobj	mice,in
R4134	T6687	T6685	cc	and,nontransgenic
R4135	T6688	T6689	compound	tet,off
R4136	T6689	T6685	conj	off,nontransgenic
R4137	T6690	T6689	punct	-,off
R4138	T6691	T6686	compound	APP,mice
R4139	T6692	T6676	advcl	using,confirmed
R4140	T6693	T6694	det	an,antibody
R4141	T6694	T6692	dobj	antibody,using
R4142	T6695	T6696	dep	that,recognizes
R4143	T6696	T6694	relcl	recognizes,antibody
R4144	T6697	T6698	preconj	both,APP
R4145	T6698	T6696	dobj	APP,recognizes
R4146	T6699	T6698	amod	endogenous,APP
R4147	T6700	T6698	punct	(,APP
R4148	T6701	T6698	cc	and,APP
R4149	T6702	T6703	nmod	amyloid,protein
R4150	T6703	T6698	conj	protein,APP
R4151	T6704	T6705	npadvmod	precursor,like
R4152	T6705	T6703	amod	like,protein
R4153	T6706	T6705	punct	-,like
R4154	T6707	T6703	nummod	2,protein
R4155	T6708	T6703	punct	),protein
R4156	T6709	T6698	cc	and,APP
R4157	T6710	T6711	det	the,protein
R4158	T6711	T6698	conj	protein,APP
R4159	T6712	T6711	amod	transgenic,protein
R4160	T6713	T6714	punct	(,1D
R4161	T6714	T6711	parataxis	1D,protein
R4162	T6715	T6716	amod	monoclonal,22C11
R4163	T6716	T6714	dep	22C11,1D
R4164	T6717	T6716	compound	antibody,22C11
R4165	T6718	T6714	punct	;,1D
R4166	T6719	T6714	compound	Figure,1D
R4167	T6720	T6714	punct	),1D
R4168	T6721	T6676	punct	.,confirmed
R4169	T6723	T6724	advmod	Importantly,showed
R4170	T6725	T6724	punct	", ",showed
R4171	T6726	T6727	det	all,lines
R4172	T6727	T6724	nsubj	lines,showed
R4173	T6728	T6727	nummod	four,lines
R4174	T6729	T6727	amod	new,lines
R4175	T6730	T6727	prep	of,lines
R4176	T6731	T6732	compound	tet,off
R4177	T6732	T6734	compound	off,mice
R4178	T6733	T6732	punct	-,off
R4179	T6734	T6730	pobj	mice,of
R4180	T6735	T6734	compound	APP,mice
R4181	T6736	T6737	advmod	nearly,complete
R4182	T6737	T6738	amod	complete,suppression
R4183	T6738	T6724	dobj	suppression,showed
R4184	T6739	T6738	prep	of,suppression
R4185	T6740	T6741	det	the,transgene
R4186	T6741	T6739	pobj	transgene,of
R4187	T6742	T6724	prep	following,showed
R4188	T6743	T6744	compound	dox,treatment
R4189	T6744	T6742	pobj	treatment,following
R4190	T6745	T6746	punct	(,Figures
R4191	T6746	T6724	parataxis	Figures,showed
R4192	T6747	T6746	nummod	1,Figures
R4193	T6748	T6746	cc	and,Figures
R4194	T6749	T6750	compound	Figure,S1
R4195	T6750	T6746	conj	S1,Figures
R4196	T6751	T6746	punct	),Figures
R4197	T6752	T6724	punct	.,showed
R4198	T6754	T6755	nsubj	We,focused
R4199	T6756	T6755	prep	on,focused
R4200	T6757	T6756	pobj	one,on
R4201	T6758	T6757	prep	of,one
R4202	T6759	T6760	det	the,lines
R4203	T6760	T6758	pobj	lines,of
R4204	T6761	T6760	nummod	four,lines
R4205	T6762	T6757	punct	", ",one
R4206	T6763	T6757	appos	line,one
R4207	T6764	T6763	nummod	107,line
R4208	T6765	T6755	punct	", ",focused
R4209	T6766	T6767	aux	to,examine
R4210	T6767	T6755	advcl	examine,focused
R4211	T6768	T6767	prep	in,examine
R4212	T6769	T6770	amod	more,detail
R4213	T6770	T6768	pobj	detail,in
R4214	T6771	T6772	det	the,dependence
R4215	T6772	T6767	dobj	dependence,examine
R4216	T6773	T6772	compound	time,dependence
R4217	T6774	T6772	cc	and,dependence
R4218	T6775	T6772	conj	extent,dependence
R4219	T6776	T6775	prep	of,extent
R4220	T6777	T6778	compound	transgene,suppression
R4221	T6778	T6776	pobj	suppression,of
R4222	T6779	T6772	prep	following,dependence
R4223	T6780	T6781	preconj	either,treatment
R4224	T6781	T6779	pobj	treatment,following
R4225	T6782	T6781	amod	acute,treatment
R4226	T6783	T6782	cc	or,acute
R4227	T6784	T6782	conj	chronic,acute
R4228	T6785	T6781	prep	with,treatment
R4229	T6786	T6785	pobj	dox,with
R4230	T6787	T6755	punct	.,focused
R4231	T6789	T6790	nummod	Two,groups
R4232	T6790	T6794	nsubjpass	groups,compared
R4233	T6791	T6792	npadvmod	dox,treated
R4234	T6792	T6790	amod	treated,groups
R4235	T6793	T6792	punct	-,treated
R4236	T6794	T6796	ccomp	compared,harvested
R4237	T6795	T6794	auxpass	were,compared
R4238	T6797	T6794	prep	to,compared
R4239	T6798	T6799	nummod	two,groups
R4240	T6799	T6797	pobj	groups,to
R4241	T6800	T6799	amod	untreated,groups
R4242	T6801	T6796	punct	: ,harvested
R4243	T6802	T6803	nummod	one,group
R4244	T6803	T6804	nsubjpass	group,born
R4245	T6804	T6796	ccomp	born,harvested
R4246	T6805	T6803	prep	of,group
R4247	T6806	T6805	pobj	mice,of
R4248	T6807	T6804	auxpass	was,born
R4249	T6808	T6804	cc	and,born
R4250	T6809	T6804	conj	raised,born
R4251	T6810	T6809	prep	on,raised
R4252	T6811	T6810	pobj	dox,on
R4253	T6812	T6796	punct	", ",harvested
R4254	T6813	T6814	det	a,group
R4255	T6814	T6816	nsubjpass	group,treated
R4256	T6815	T6814	amod	second,group
R4257	T6816	T6796	ccomp	treated,harvested
R4258	T6817	T6816	auxpass	was,treated
R4259	T6818	T6816	prep	with,treated
R4260	T6819	T6818	pobj	dox,with
R4261	T6820	T6816	prep	for,treated
R4262	T6821	T6822	nummod	2,wk
R4263	T6822	T6820	pobj	wk,for
R4264	T6823	T6816	advcl	starting,treated
R4265	T6824	T6823	prep	at,starting
R4266	T6825	T6826	nummod	1,mo
R4267	T6826	T6824	pobj	mo,at
R4268	T6827	T6826	prep	of,mo
R4269	T6828	T6827	pobj	age,of
R4270	T6829	T6830	punct	(,wk
R4271	T6830	T6816	parataxis	wk,treated
R4272	T6831	T6830	nummod	4,wk
R4273	T6832	T6830	punct	+,wk
R4274	T6833	T6834	nummod	2,wk
R4275	T6834	T6835	compound	wk,dox
R4276	T6835	T6830	appos	dox,wk
R4277	T6836	T6830	punct	),wk
R4278	T6837	T6796	punct	;,harvested
R4279	T6838	T6839	nummod	two,groups
R4280	T6839	T6796	nsubjpass	groups,harvested
R4281	T6840	T6839	amod	untreated,groups
R4282	T6841	T6839	acl	kept,groups
R4283	T6842	T6841	prep	on,kept
R4284	T6843	T6844	amod	normal,chow
R4285	T6844	T6842	pobj	chow,on
R4286	T6845	T6796	auxpass	were,harvested
R4287	T6846	T6796	prep	at,harvested
R4288	T6847	T6848	preconj	either,4
R4289	T6848	T6849	nummod	4,wk
R4290	T6849	T6846	pobj	wk,at
R4291	T6850	T6848	cc	or,4
R4292	T6851	T6848	conj	6,4
R4293	T6852	T6849	prep	of,wk
R4294	T6853	T6852	pobj	age,of
R4295	T6854	T6796	punct	.,harvested
R4296	T6856	T6857	nsubj	Animals,harbored
R4297	T6858	T6856	acl	born,Animals
R4298	T6859	T6858	cc	and,born
R4299	T6860	T6858	conj	raised,born
R4300	T6861	T6860	prep	on,raised
R4301	T6862	T6861	pobj	dox,on
R4302	T6863	T6864	det	no,APP
R4303	T6864	T6857	dobj	APP,harbored
R4304	T6865	T6864	amod	transgenic,APP
R4305	T6866	T6867	punct	(,1A
R4306	T6867	T6857	parataxis	1A,harbored
R4307	T6868	T6867	compound	Figure,1A
R4308	T6869	T6867	punct	),1A
R4309	T6870	T6857	punct	.,harbored
R4310	T6872	T6873	prep	Following,reduced
R4311	T6874	T6875	advmod	as,2
R4312	T6875	T6878	nummod	2,wk
R4313	T6876	T6875	amod	little,2
R4314	T6877	T6875	quantmod	as,2
R4315	T6878	T6872	pobj	wk,Following
R4316	T6879	T6878	prep	of,wk
R4317	T6880	T6881	compound	dox,treatment
R4318	T6881	T6879	pobj	treatment,of
R4319	T6882	T6873	punct	", ",reduced
R4320	T6883	T6884	amod	transgenic,expression
R4321	T6884	T6873	nsubjpass	expression,reduced
R4322	T6885	T6884	compound	APP,expression
R4323	T6886	T6873	auxpass	was,reduced
R4324	T6887	T6873	prep	by,reduced
R4325	T6888	T6889	amod	more,95
R4326	T6889	T6891	nummod	95,%
R4327	T6890	T6889	quantmod	than,95
R4328	T6891	T6887	pobj	%,by
R4329	T6892	T6873	prep	compared,reduced
R4330	T6893	T6892	prep	to,compared
R4331	T6894	T6895	amod	pre-dox,levels
R4332	T6895	T6893	pobj	levels,to
R4333	T6896	T6873	punct	.,reduced
R4334	T6898	T6899	det	The,expression
R4335	T6899	T6901	nsubj	expression,represents
R4336	T6900	T6899	amod	residual,expression
R4337	T6902	T6899	acl	remaining,expression
R4338	T6903	T6902	prep	in,remaining
R4339	T6904	T6905	advmod	acutely,treated
R4340	T6905	T6906	amod	treated,mice
R4341	T6906	T6903	pobj	mice,in
R4342	T6907	T6908	amod	less,4
R4343	T6908	T6910	nummod	4,%
R4344	T6909	T6908	quantmod	than,4
R4345	T6910	T6901	dobj	%,represents
R4346	T6911	T6910	prep	of,%
R4347	T6912	T6913	det	the,protein
R4348	T6913	T6911	pobj	protein,of
R4349	T6914	T6913	amod	transgenic,protein
R4350	T6915	T6913	acl	produced,protein
R4351	T6916	T6915	prep	in,produced
R4352	T6917	T6918	det	the,absence
R4353	T6918	T6916	pobj	absence,in
R4354	T6919	T6918	prep	of,absence
R4355	T6920	T6919	pobj	dox,of
R4356	T6921	T6922	punct	(,1C
R4357	T6922	T6915	parataxis	1C,produced
R4358	T6923	T6922	compound	Figure,1C
R4359	T6924	T6922	punct	),1C
R4360	T6925	T6901	punct	", ",represents
R4361	T6926	T6901	cc	and,represents
R4362	T6927	T6928	advmod	likely,results
R4363	T6928	T6901	conj	results,represents
R4364	T6929	T6928	prep	from,results
R4365	T6930	T6931	amod	slight,leakage
R4366	T6931	T6929	pobj	leakage,from
R4367	T6932	T6931	prep	at,leakage
R4368	T6933	T6934	det	the,level
R4369	T6934	T6932	pobj	level,at
R4370	T6935	T6934	prep	of,level
R4371	T6936	T6935	pobj	transcription,of
R4372	T6937	T6938	punct	(,shown
R4373	T6938	T6928	parataxis	shown,results
R4374	T6939	T6938	nsubj	data,shown
R4375	T6940	T6938	neg	not,shown
R4376	T6941	T6938	punct	),shown
R4377	T6942	T6901	punct	.,represents
R4378	T6944	T6945	advmod	Importantly,was
R4379	T6946	T6945	punct	", ",was
R4380	T6947	T6948	det	the,amount
R4381	T6948	T6945	nsubj	amount,was
R4382	T6949	T6948	amod	total,amount
R4383	T6950	T6948	prep	of,amount
R4384	T6951	T6950	pobj	APP,of
R4385	T6952	T6953	punct	(,endogenous
R4386	T6953	T6951	parataxis	endogenous,APP
R4387	T6954	T6953	cc	plus,endogenous
R4388	T6955	T6953	conj	transgenic,endogenous
R4389	T6956	T6953	punct	),endogenous
R4390	T6957	T6951	cc	and,APP
R4391	T6958	T6959	amod	related,APLPs
R4392	T6959	T6951	conj	APLPs,APP
R4393	T6960	T6948	prep	in,amount
R4394	T6961	T6962	preconj	both,acute
R4395	T6962	T6963	amod	acute,animals
R4396	T6963	T6960	pobj	animals,in
R4397	T6964	T6962	cc	and,acute
R4398	T6965	T6966	advmod	chronically,treated
R4399	T6966	T6962	conj	treated,acute
R4400	T6967	T6968	advmod	statistically,indistinguishable
R4401	T6968	T6945	acomp	indistinguishable,was
R4402	T6969	T6968	prep	from,indistinguishable
R4403	T6970	T6969	pobj	that,from
R4404	T6971	T6970	prep	in,that
R4405	T6972	T6973	amod	nontransgenic,mice
R4406	T6973	T6971	pobj	mice,in
R4407	T6974	T6975	punct	(,1D
R4408	T6975	T6945	parataxis	1D,was
R4409	T6976	T6975	compound	Figure,1D
R4410	T6977	T6975	punct	;,1D
R4411	T6978	T6979	amod	statistical,analyses
R4412	T6979	T6980	nsubjpass	analyses,presented
R4413	T6980	T6975	ccomp	presented,1D
R4414	T6981	T6979	prep	for,analyses
R4415	T6982	T6981	pobj	experiments,for
R4416	T6983	T6982	prep	throughout,experiments
R4417	T6984	T6985	det	the,study
R4418	T6985	T6983	pobj	study,throughout
R4419	T6986	T6980	auxpass	are,presented
R4420	T6987	T6980	prep	in,presented
R4421	T6988	T6989	det	the,legends
R4422	T6989	T6987	pobj	legends,in
R4423	T6990	T6989	amod	accompanying,legends
R4424	T6991	T6989	compound	figure,legends
R4425	T6992	T6975	punct	),1D
R4426	T6993	T6945	punct	.,was
R4427	T6995	T6996	aux	To,ensure
R4428	T6996	T6997	advcl	ensure,measured
R4429	T6998	T6999	mark	that,suppressed
R4430	T6999	T6996	ccomp	suppressed,ensure
R4431	T7000	T7001	compound	Aβ,production
R4432	T7001	T6999	nsubjpass	production,suppressed
R4433	T7002	T6999	auxpass	was,suppressed
R4434	T7003	T6999	prep	in,suppressed
R4435	T7004	T7003	pobj	concert,in
R4436	T7005	T7004	prep	with,concert
R4437	T7006	T7007	det	the,inhibition
R4438	T7007	T7005	pobj	inhibition,with
R4439	T7008	T7009	npadvmod	dox,mediated
R4440	T7009	T7007	amod	mediated,inhibition
R4441	T7010	T7009	punct	-,mediated
R4442	T7011	T7007	prep	of,inhibition
R4443	T7012	T7013	poss	its,precursor
R4444	T7013	T7011	pobj	precursor,of
R4445	T7014	T7015	compound	APPswe,ind
R4446	T7015	T7013	appos	ind,precursor
R4447	T7016	T7015	punct	/,ind
R4448	T7017	T6997	punct	", ",measured
R4449	T7018	T6997	nsubj	we,measured
R4450	T7019	T7020	nmod	Aβ40,levels
R4451	T7020	T6997	dobj	levels,measured
R4452	T7021	T7019	cc	and,Aβ40
R4453	T7022	T7019	conj	Aβ42,Aβ40
R4454	T7023	T6997	prep	by,measured
R4455	T7024	T7023	pobj	ELISA,by
R4456	T7025	T6997	prep	in,measured
R4457	T7026	T7027	compound	forebrain,homogenates
R4458	T7027	T7025	pobj	homogenates,in
R4459	T7028	T7027	prep	from,homogenates
R4460	T7029	T7030	amod	young,animals
R4461	T7030	T7028	pobj	animals,from
R4462	T7031	T7032	compound	tet,off
R4463	T7032	T7030	compound	off,animals
R4464	T7033	T7032	punct	-,off
R4465	T7034	T6997	punct	.,measured
R4466	T7036	T7037	prep	At,lacked
R4467	T7038	T7039	nummod	1,mo
R4468	T7039	T7036	pobj	mo,At
R4469	T7040	T7039	prep	of,mo
R4470	T7041	T7040	pobj	age,of
R4471	T7042	T7037	punct	", ",lacked
R4472	T7043	T7044	det	the,mice
R4473	T7044	T7037	nsubj	mice,lacked
R4474	T7045	T7046	amod	visible,aggregates
R4475	T7046	T7037	dobj	aggregates,lacked
R4476	T7047	T7046	compound	amyloid,aggregates
R4477	T7048	T7049	dep	that,act
R4478	T7049	T7046	relcl	act,aggregates
R4479	T7050	T7049	aux	might,act
R4480	T7051	T7049	prep	as,act
R4481	T7052	T7053	det	an,reservoir
R4482	T7053	T7051	pobj	reservoir,as
R4483	T7054	T7053	amod	intractable,reservoir
R4484	T7055	T7053	prep	of,reservoir
R4485	T7056	T7055	pobj	peptide,of
R4486	T7057	T7053	acl	remaining,reservoir
R4487	T7058	T7057	prep	in,remaining
R4488	T7059	T7060	det	the,brain
R4489	T7060	T7058	pobj	brain,in
R4490	T7061	T7062	prep	after,suppressed
R4491	T7062	T7057	advcl	suppressed,remaining
R4492	T7063	T7064	det	the,transgene
R4493	T7064	T7062	nsubjpass	transgene,suppressed
R4494	T7065	T7062	aux	had,suppressed
R4495	T7066	T7062	auxpass	been,suppressed
R4496	T7067	T7037	punct	.,lacked
R4497	T7069	T7070	aux	To,ensure
R4498	T7070	T7072	advcl	ensure,performed
R4499	T7071	T7070	advmod	further,ensure
R4500	T7073	T7074	nsubj	we,detect
R4501	T7074	T7070	advcl	detect,ensure
R4502	T7075	T7074	aux	could,detect
R4503	T7076	T7077	det	any,aggregates
R4504	T7077	T7074	dobj	aggregates,detect
R4505	T7078	T7077	amod	such,aggregates
R4506	T7079	T7077	amod	insoluble,aggregates
R4507	T7080	T7081	dep	that,bias
R4508	T7081	T7077	relcl	bias,aggregates
R4509	T7082	T7081	aux	might,bias
R4510	T7083	T7084	poss	our,measure
R4511	T7084	T7081	dobj	measure,bias
R4512	T7085	T7084	prep	of,measure
R4513	T7086	T7085	pobj	changes,of
R4514	T7087	T7081	prep	in,bias
R4515	T7088	T7089	compound	peptide,synthesis
R4516	T7089	T7087	pobj	synthesis,in
R4517	T7090	T7072	punct	", ",performed
R4518	T7091	T7072	nsubj	we,performed
R4519	T7092	T7093	det	a,extraction
R4520	T7093	T7072	dobj	extraction,performed
R4521	T7094	T7093	amod	sequential,extraction
R4522	T7095	T7096	nummod	three,step
R4523	T7096	T7093	compound	step,extraction
R4524	T7097	T7096	punct	-,step
R4525	T7098	T7093	prep	with,extraction
R4526	T7099	T7098	pobj	PBS,with
R4527	T7100	T7099	punct	", ",PBS
R4528	T7101	T7102	nummod	2,%
R4529	T7102	T7103	compound	%,SDS
R4530	T7103	T7099	conj	SDS,PBS
R4531	T7104	T7103	punct	", ",SDS
R4532	T7105	T7103	cc	and,SDS
R4533	T7106	T7107	nummod	70,%
R4534	T7107	T7108	compound	%,FA
R4535	T7108	T7103	conj	FA,SDS
R4536	T7109	T7110	dep	that,separate
R4537	T7110	T7093	relcl	separate,extraction
R4538	T7111	T7110	aux	would,separate
R4539	T7112	T7110	dobj	peptides,separate
R4540	T7113	T7110	prep	by,separate
R4541	T7114	T7113	pobj	solubility,by
R4542	T7115	T7072	punct	.,performed
R4543	T7117	T7118	nsubj	We,compared
R4544	T7119	T7120	det	the,levels
R4545	T7120	T7118	dobj	levels,compared
R4546	T7121	T7120	prep	of,levels
R4547	T7122	T7123	amod	human,transgene
R4548	T7123	T7124	npadvmod	transgene,derived
R4549	T7124	T7126	amod	derived,Aβ40
R4550	T7125	T7124	punct	-,derived
R4551	T7126	T7121	pobj	Aβ40,of
R4552	T7127	T7126	cc	and,Aβ40
R4553	T7128	T7126	conj	Aβ42,Aβ40
R4554	T7129	T7120	prep	in,levels
R4555	T7130	T7131	amod	untreated,mice
R4556	T7131	T7129	pobj	mice,in
R4557	T7132	T7120	prep	at,levels
R4558	T7133	T7134	nummod	4,wk
R4559	T7134	T7132	pobj	wk,at
R4560	T7135	T7133	cc	and,4
R4561	T7136	T7133	conj	6,4
R4562	T7137	T7134	prep	of,wk
R4563	T7138	T7137	pobj	age,of
R4564	T7139	T7118	prep	to,compared
R4565	T7140	T7139	pobj	animals,to
R4566	T7141	T7142	dep	that,born
R4567	T7142	T7140	advcl	born,animals
R4568	T7143	T7142	aux	had,born
R4569	T7144	T7142	preconj	either,born
R4570	T7145	T7142	auxpass	been,born
R4571	T7146	T7142	cc	and,born
R4572	T7147	T7142	conj	raised,born
R4573	T7148	T7147	prep	on,raised
R4574	T7149	T7148	pobj	dox,on
R4575	T7150	T7142	cc	or,born
R4576	T7151	T7152	dep	that,left
R4577	T7152	T7142	conj	left,born
R4578	T7153	T7152	aux	had,left
R4579	T7154	T7152	auxpass	been,left
R4580	T7155	T7152	oprd	untreated,left
R4581	T7156	T7152	prep	for,left
R4582	T7157	T7158	nummod	4,wk
R4583	T7158	T7156	pobj	wk,for
R4584	T7159	T7152	cc	and,left
R4585	T7160	T7161	advmod	then,placed
R4586	T7161	T7152	conj	placed,left
R4587	T7162	T7161	prep	on,placed
R4588	T7163	T7164	compound	dox,chow
R4589	T7164	T7162	pobj	chow,on
R4590	T7165	T7161	prep	for,placed
R4591	T7166	T7167	nummod	2,wk
R4592	T7167	T7165	pobj	wk,for
R4593	T7168	T7169	amod	prior,to
R4594	T7169	T7167	prep	to,wk
R4595	T7170	T7169	pobj	harvest,to
R4596	T7171	T7172	punct	(,line
R4597	T7172	T7161	parataxis	line,placed
R4598	T7173	T7174	det	the,groups
R4599	T7174	T7172	dep	groups,line
R4600	T7175	T7174	amod	same,groups
R4601	T7176	T7174	acl	described,groups
R4602	T7177	T7176	advmod	above,described
R4603	T7178	T7176	prep	for,described
R4604	T7179	T7180	compound	immunoblot,analysis
R4605	T7180	T7178	pobj	analysis,for
R4606	T7181	T7180	prep	of,analysis
R4607	T7182	T7183	compound	APPswe,ind
R4608	T7183	T7185	compound	ind,levels
R4609	T7184	T7183	punct	/,ind
R4610	T7185	T7181	pobj	levels,of
R4611	T7186	T7172	punct	", ",line
R4612	T7187	T7172	nummod	107,line
R4613	T7188	T7172	punct	),line
R4614	T7189	T7118	punct	.,compared
R4615	T7191	T7192	advcl	Consistent,found
R4616	T7193	T7191	prep	with,Consistent
R4617	T7194	T7195	det	the,reduction
R4618	T7195	T7193	pobj	reduction,with
R4619	T7196	T7195	prep	in,reduction
R4620	T7197	T7198	amod	full,length
R4621	T7198	T7200	compound	length,synthesis
R4622	T7199	T7198	punct	-,length
R4623	T7200	T7196	pobj	synthesis,in
R4624	T7201	T7202	compound	APPswe,ind
R4625	T7202	T7200	compound	ind,synthesis
R4626	T7203	T7202	punct	/,ind
R4627	T7204	T7195	acl	shown,reduction
R4628	T7205	T7204	agent	by,shown
R4629	T7206	T7205	pobj	immunoblot,by
R4630	T7207	T7208	punct	(,see
R4631	T7208	T7191	parataxis	see,Consistent
R4632	T7209	T7208	dobj	Figure,see
R4633	T7210	T7209	nummod	1,Figure
R4634	T7211	T7208	punct	),see
R4635	T7212	T7192	punct	", ",found
R4636	T7213	T7192	nsubj	we,found
R4637	T7214	T7215	mark	that,suppressed
R4638	T7215	T7192	ccomp	suppressed,found
R4639	T7216	T7217	npadvmod	transgene,derived
R4640	T7217	T7219	amod	derived,levels
R4641	T7218	T7217	punct	-,derived
R4642	T7219	T7215	nsubjpass	levels,suppressed
R4643	T7220	T7219	compound	Aβ,levels
R4644	T7221	T7215	auxpass	were,suppressed
R4645	T7222	T7215	advmod	completely,suppressed
R4646	T7223	T7215	prep	in,suppressed
R4647	T7224	T7223	pobj	animals,in
R4648	T7225	T7224	acl	born,animals
R4649	T7226	T7225	cc	and,born
R4650	T7227	T7225	conj	raised,born
R4651	T7228	T7227	prep	on,raised
R4652	T7229	T7228	pobj	dox,on
R4653	T7230	T7215	punct	", ",suppressed
R4654	T7231	T7215	cc	and,suppressed
R4655	T7232	T7233	auxpass	were,reduced
R4656	T7233	T7215	conj	reduced,suppressed
R4657	T7234	T7233	advmod	sharply,reduced
R4658	T7235	T7233	prep	following,reduced
R4659	T7236	T7237	amod	acute,treatment
R4660	T7237	T7235	pobj	treatment,following
R4661	T7238	T7239	punct	(,wk
R4662	T7239	T7236	parataxis	wk,acute
R4663	T7240	T7239	nummod	2,wk
R4664	T7241	T7239	punct	),wk
R4665	T7242	T7237	amod	antibiotic,treatment
R4666	T7243	T7192	punct	.,found
R4667	T7245	T7246	prep	Compared,dropped
R4668	T7247	T7245	prep	to,Compared
R4669	T7248	T7249	det	the,levels
R4670	T7249	T7247	pobj	levels,to
R4671	T7250	T7249	prep	in,levels
R4672	T7251	T7252	amod	untreated,mice
R4673	T7252	T7250	pobj	mice,in
R4674	T7253	T7254	nummod	4,wk
R4675	T7254	T7256	npadvmod	wk,old
R4676	T7255	T7254	punct	-,wk
R4677	T7256	T7252	amod	old,mice
R4678	T7257	T7256	punct	-,old
R4679	T7258	T7246	punct	", ",dropped
R4680	T7259	T7260	npadvmod	PBS,soluble
R4681	T7260	T7262	amod	soluble,Aβ42
R4682	T7261	T7260	punct	-,soluble
R4683	T7262	T7246	nsubj	Aβ42,dropped
R4684	T7263	T7246	prep	by,dropped
R4685	T7264	T7265	nummod	95.2,%
R4686	T7265	T7263	pobj	%,by
R4687	T7266	T7246	prep	following,dropped
R4688	T7267	T7268	nummod	2,wk
R4689	T7268	T7266	pobj	wk,following
R4690	T7269	T7268	prep	of,wk
R4691	T7270	T7271	compound	dox,treatment
R4692	T7271	T7269	pobj	treatment,of
R4693	T7272	T7246	cc	and,dropped
R4694	T7273	T7274	prep	by,with
R4695	T7274	T7246	conj	with,dropped
R4696	T7275	T7276	nummod	99.2,%
R4697	T7276	T7273	pobj	%,by
R4698	T7277	T7278	amod	chronic,treatment
R4699	T7278	T7274	pobj	treatment,with
R4700	T7279	T7280	punct	(,2A
R4701	T7280	T7274	parataxis	2A,with
R4702	T7281	T7280	compound	Figure,2A
R4703	T7282	T7280	punct	),2A
R4704	T7283	T7246	punct	.,dropped
R4705	T7285	T7286	advmod	Similarly,decreased
R4706	T7287	T7286	punct	", ",decreased
R4707	T7288	T7289	npadvmod	SDS,soluble
R4708	T7289	T7291	amod	soluble,Aβ42
R4709	T7290	T7289	punct	-,soluble
R4710	T7291	T7286	nsubj	Aβ42,decreased
R4711	T7292	T7286	prep	by,decreased
R4712	T7293	T7294	nummod	75.2,%
R4713	T7294	T7292	pobj	%,by
R4714	T7295	T7294	cc	and,%
R4715	T7296	T7297	nummod	94.8,%
R4716	T7297	T7294	conj	%,%
R4717	T7298	T7286	prep	following,decreased
R4718	T7299	T7300	nummod	2,wk
R4719	T7300	T7302	nmod	wk,treatment
R4720	T7301	T7300	punct	-,wk
R4721	T7302	T7298	pobj	treatment,following
R4722	T7303	T7300	cc	or,wk
R4723	T7304	T7300	conj	lifelong,wk
R4724	T7305	T7302	compound	dox,treatment
R4725	T7306	T7307	punct	(,2B
R4726	T7307	T7286	parataxis	2B,decreased
R4727	T7308	T7307	compound	Figure,2B
R4728	T7309	T7307	punct	),2B
R4729	T7310	T7286	punct	.,decreased
R4730	T7312	T7313	advmod	Only,fraction
R4731	T7313	T7316	nsubj	fraction,revealed
R4732	T7314	T7313	det	the,fraction
R4733	T7315	T7313	compound	FA,fraction
R4734	T7317	T7318	det	a,pool
R4735	T7318	T7316	dobj	pool,revealed
R4736	T7319	T7318	amod	small,pool
R4737	T7320	T7321	npadvmod	dox,resistant
R4738	T7321	T7318	amod	resistant,pool
R4739	T7322	T7321	punct	-,resistant
R4740	T7323	T7318	prep	of,pool
R4741	T7324	T7323	pobj	peptide,of
R4742	T7325	T7318	prep	in,pool
R4743	T7326	T7327	advmod	acutely,treated
R4744	T7327	T7328	amod	treated,animals
R4745	T7328	T7325	pobj	animals,in
R4746	T7329	T7330	dep	that,believe
R4747	T7330	T7318	relcl	believe,pool
R4748	T7331	T7330	nsubj	we,believe
R4749	T7332	T7330	advcl	represents,believe
R4750	T7333	T7334	amod	stable,aggregates
R4751	T7334	T7332	dobj	aggregates,represents
R4752	T7335	T7334	amod	predeposit,aggregates
R4753	T7336	T7337	dep	that,accumulated
R4754	T7337	T7334	relcl	accumulated,aggregates
R4755	T7338	T7337	aux	have,accumulated
R4756	T7339	T7337	advmod	already,accumulated
R4757	T7340	T7337	prep	by,accumulated
R4758	T7341	T7342	nummod	4,wk
R4759	T7342	T7340	pobj	wk,by
R4760	T7343	T7342	prep	of,wk
R4761	T7344	T7343	pobj	age,of
R4762	T7345	T7346	advmod	when,begun
R4763	T7346	T7337	advcl	begun,accumulated
R4764	T7347	T7346	nsubjpass	treatment,begun
R4765	T7348	T7346	auxpass	was,begun
R4766	T7349	T7350	punct	(,2C
R4767	T7350	T7316	parataxis	2C,revealed
R4768	T7351	T7350	compound	Figure,2C
R4769	T7352	T7350	punct	),2C
R4770	T7353	T7316	punct	.,revealed
R4771	T7355	T7356	advmod	Indeed,harbor
R4772	T7357	T7356	punct	", ",harbor
R4773	T7358	T7356	nsubj	animals,harbor
R4774	T7359	T7360	dep	that,born
R4775	T7360	T7358	relcl	born,animals
R4776	T7361	T7360	auxpass	were,born
R4777	T7362	T7360	cc	and,born
R4778	T7363	T7360	conj	raised,born
R4779	T7364	T7363	prep	on,raised
R4780	T7365	T7364	pobj	dox,on
R4781	T7366	T7356	aux	did,harbor
R4782	T7367	T7356	neg	not,harbor
R4783	T7368	T7369	det	this,reservoir
R4784	T7369	T7356	dobj	reservoir,harbor
R4785	T7370	T7369	prep	of,reservoir
R4786	T7371	T7372	npadvmod	treatment,resistant
R4787	T7372	T7374	amod	resistant,peptide
R4788	T7373	T7372	punct	-,resistant
R4789	T7374	T7370	pobj	peptide,of
R4790	T7375	T7356	punct	", ",harbor
R4791	T7376	T7356	prep	with,harbor
R4792	T7377	T7378	nummod	96.3,%
R4793	T7378	T7379	npadvmod	%,less
R4794	T7379	T7380	amod	less,Aβ42
R4795	T7380	T7376	pobj	Aβ42,with
R4796	T7381	T7380	prep	than,Aβ42
R4797	T7382	T7383	amod	untreated,mice
R4798	T7383	T7381	pobj	mice,than
R4799	T7384	T7385	nummod	4,wk
R4800	T7385	T7387	npadvmod	wk,old
R4801	T7386	T7385	punct	-,wk
R4802	T7387	T7383	amod	old,mice
R4803	T7388	T7387	punct	-,old
R4804	T7389	T7356	punct	.,harbor
R4805	T7391	T7392	nsubj	Measurement,demonstrated
R4806	T7393	T7391	prep	of,Measurement
R4807	T7394	T7395	amod	total,Aβ
R4808	T7395	T7393	pobj	Aβ,of
R4809	T7396	T7391	prep	in,Measurement
R4810	T7397	T7398	advmod	chronically,treated
R4811	T7398	T7399	amod	treated,mice
R4812	T7399	T7396	pobj	mice,in
R4813	T7400	T7391	punct	", ",Measurement
R4814	T7401	T7391	prep	including,Measurement
R4815	T7402	T7403	amod	endogenous,peptide
R4816	T7403	T7401	pobj	peptide,including
R4817	T7404	T7402	cc	and,endogenous
R4818	T7405	T7406	npadvmod	transgene,derived
R4819	T7406	T7402	conj	derived,endogenous
R4820	T7407	T7406	punct	-,derived
R4821	T7408	T7392	punct	", ",demonstrated
R4822	T7409	T7410	mark	that,reduced
R4823	T7410	T7392	ccomp	reduced,demonstrated
R4824	T7411	T7412	compound	Aβ,levels
R4825	T7412	T7410	nsubjpass	levels,reduced
R4826	T7413	T7412	prep	in,levels
R4827	T7414	T7415	compound	tet,off
R4828	T7415	T7417	compound	off,mice
R4829	T7416	T7415	punct	-,off
R4830	T7417	T7413	pobj	mice,in
R4831	T7418	T7417	compound	APP,mice
R4832	T7419	T7410	auxpass	were,reduced
R4833	T7420	T7410	prep	to,reduced
R4834	T7421	T7422	det	the,level
R4835	T7422	T7420	pobj	level,to
R4836	T7423	T7422	prep	of,level
R4837	T7424	T7425	amod	endogenous,peptide
R4838	T7425	T7423	pobj	peptide,of
R4839	T7426	T7422	acl	found,level
R4840	T7427	T7426	prep	in,found
R4841	T7428	T7429	amod	nontransgenic,animals
R4842	T7429	T7427	pobj	animals,in
R4843	T7430	T7431	punct	(,2D
R4844	T7431	T7410	parataxis	2D,reduced
R4845	T7432	T7431	compound	Figure,2D
R4846	T7433	T7431	punct	),2D
R4847	T7434	T7392	punct	.,demonstrated
R4848	T7436	T7437	advcl	Taken,indicate
R4849	T7438	T7436	advmod	together,Taken
R4850	T7439	T7436	prep	with,Taken
R4851	T7440	T7441	det	the,data
R4852	T7441	T7439	pobj	data,with
R4853	T7442	T7441	compound	immunoblotting,data
R4854	T7443	T7441	prep	for,data
R4855	T7444	T7445	amod	full,length
R4856	T7445	T7447	compound	length,ind
R4857	T7446	T7445	punct	-,length
R4858	T7447	T7443	pobj	ind,for
R4859	T7448	T7447	compound	APPswe,ind
R4860	T7449	T7447	punct	/,ind
R4861	T7450	T7437	punct	", ",indicate
R4862	T7451	T7452	det	the,measurements
R4863	T7452	T7437	nsubj	measurements,indicate
R4864	T7453	T7452	compound	ELISA,measurements
R4865	T7454	T7455	mark	that,leads
R4866	T7455	T7437	ccomp	leads,indicate
R4867	T7543	T7544	advmod	as,young
R4868	T7456	T7457	npadvmod	dox,mediated
R4869	T7544	T7542	amod	young,mice
R4870	T7545	T7544	prep	as,young
R4871	T7457	T7459	amod	mediated,suppression
R4872	T7546	T7547	nummod	8,wk
R4873	T7547	T7545	pobj	wk,as
R4874	T7548	T7547	prep	of,wk
R4875	T7458	T7457	punct	-,mediated
R4876	T7549	T7548	pobj	age,of
R4877	T7550	T7551	punct	(,shown
R4878	T7459	T7455	nsubj	suppression,leads
R4879	T7551	T7539	parataxis	shown,seen
R4880	T7552	T7551	nsubj	data,shown
R4881	T7460	T7459	prep	of,suppression
R4882	T7553	T7551	neg	not,shown
R4883	T7554	T7551	punct	),shown
R4884	T7555	T7539	punct	.,seen
R4885	T7461	T7462	amod	transgenic,synthesis
R4886	T7557	T7558	nsubjpass	Plaques,limited
R4887	T7462	T7460	pobj	synthesis,of
R4888	T7559	T7558	auxpass	were,limited
R4889	T7463	T7464	compound	APPswe,ind
R4890	T7560	T7558	prep	to,limited
R4891	T7561	T7562	det	the,forebrain
R4892	T7562	T7560	pobj	forebrain,to
R4893	T7464	T7462	compound	ind,synthesis
R4894	T7563	T7562	punct	", ",forebrain
R4895	T7564	T7562	prep	including,forebrain
R4896	T7565	T7566	det	the,cortex
R4897	T7465	T7464	punct	/,ind
R4898	T7566	T7564	pobj	cortex,including
R4899	T7567	T7566	punct	", ",cortex
R4900	T7568	T7566	conj	hippocampus,cortex
R4901	T7569	T7568	punct	", ",hippocampus
R4902	T7570	T7571	amod	olfactory,bulb
R4903	T7466	T7455	prep	to,leads
R4904	T7571	T7568	conj	bulb,hippocampus
R4905	T7572	T7571	punct	", ",bulb
R4906	T7573	T7571	cc	and,bulb
R4907	T7467	T7468	amod	parallel,reduction
R4908	T7574	T7571	conj	striatum,bulb
R4909	T7468	T7466	pobj	reduction,to
R4910	T7575	T7562	punct	", ",forebrain
R4911	T7576	T7577	advmod	where,known
R4912	T7577	T7562	relcl	known,forebrain
R4913	T7578	T7579	det	the,promoter
R4914	T7469	T7468	prep	of,reduction
R4915	T7579	T7577	nsubjpass	promoter,known
R4916	T7580	T7579	compound	CaMKIIα,promoter
R4917	T7581	T7577	auxpass	is,known
R4918	T7470	T7471	compound	Aβ,levels
R4919	T7582	T7583	aux	to,be
R4920	T7583	T7577	xcomp	be,known
R4921	T7584	T7585	advmod	most,active
R4922	T7471	T7469	pobj	levels,of
R4923	T7585	T7583	acomp	active,be
R4924	T7586	T7587	punct	[,28
R4925	T7472	T7437	punct	.,indicate
R4926	T7474	T7475	det	The,data
R4927	T7587	T7577	parataxis	28,known
R4928	T7588	T7587	nummod	16,28
R4929	T7589	T7587	punct	",",28
R4930	T7475	T7477	nsubj	data,confirmed
R4931	T7590	T7587	punct	],28
R4932	T7591	T7592	punct	(,S2
R4933	T7476	T7475	compound	ELISA,data
R4934	T7592	T7558	parataxis	S2,limited
R4935	T7593	T7592	compound	Figure,S2
R4936	T7594	T7592	punct	),S2
R4937	T7478	T7477	advmod	also,confirmed
R4938	T7595	T7558	punct	.,limited
R4939	T7597	T7598	prep	By,became
R4940	T7479	T7480	mark	that,led
R4941	T7599	T7600	nummod	6,mo
R4942	T7480	T7477	ccomp	led,confirmed
R4943	T7600	T7597	pobj	mo,By
R4944	T7601	T7600	prep	of,mo
R4945	T7602	T7601	pobj	age,of
R4946	T7481	T7480	nsubj	incorporation,led
R4947	T7603	T7598	punct	", ",became
R4948	T7604	T7605	compound	amyloid,burden
R4949	T7605	T7598	nsubj	burden,became
R4950	T7606	T7598	acomp	severe,became
R4951	T7607	T7598	punct	", ",became
R4952	T7608	T7598	advcl	covering,became
R4953	T7482	T7481	prep	of,incorporation
R4954	T7609	T7610	amod	large,areas
R4955	T7610	T7608	dobj	areas,covering
R4956	T7611	T7610	prep	of,areas
R4957	T7483	T7484	det	the,mutations
R4958	T7612	T7613	det	the,cortex
R4959	T7613	T7611	pobj	cortex,of
R4960	T7614	T7613	cc	and,cortex
R4961	T7484	T7482	pobj	mutations,of
R4962	T7615	T7613	conj	hippocampus,cortex
R4963	T7485	T7484	amod	Swedish,mutations
R4964	T7616	T7617	punct	(,S3
R4965	T7617	T7608	parataxis	S3,covering
R4966	T7618	T7617	compound	Figure,S3
R4967	T7619	T7617	punct	),S3
R4968	T7486	T7485	cc	and,Swedish
R4969	T7620	T7598	punct	.,became
R4970	T7487	T7485	conj	Indiana,Swedish
R4971	T7622	T7623	det	No,lesions
R4972	T7623	T7624	nsubjpass	lesions,seen
R4973	T7488	T7480	prep	to,led
R4974	T7625	T7624	auxpass	were,seen
R4975	T7626	T7624	prep	in,seen
R4976	T7489	T7490	amod	high,levels
R4977	T7627	T7628	det	the,cerebellum
R4978	T7628	T7626	pobj	cerebellum,in
R4979	T7629	T7628	cc	or,cerebellum
R4980	T7490	T7488	pobj	levels,to
R4981	T7630	T7631	compound	brain,stem
R4982	T7631	T7628	conj	stem,cerebellum
R4983	T7632	T7633	advmod	even,at
R4984	T7491	T7490	prep	of,levels
R4985	T7633	T7624	prep	at,seen
R4986	T7634	T7635	amod	late,ages
R4987	T7635	T7633	pobj	ages,at
R4988	T7492	T7491	pobj	Aβ42,of
R4989	T7636	T7624	punct	", ",seen
R4990	T7637	T7624	advcl	consistent,seen
R4991	T7493	T7490	punct	", ",levels
R4992	T7638	T7637	prep	with,consistent
R4993	T7639	T7640	npadvmod	CaMKIIα,controlled
R4994	T7640	T7642	amod	controlled,expression
R4995	T7494	T7495	dep	which,predicted
R4996	T7641	T7640	punct	-,controlled
R4997	T7642	T7638	pobj	expression,with
R4998	T7643	T7642	compound	transgene,expression
R4999	T7495	T7490	relcl	predicted,levels
R5000	T7644	T7624	punct	.,seen
R5001	T7496	T7495	nsubj	we,predicted
R5002	T7646	T7647	prep	Unlike,are
R5003	T7648	T7649	dep	what,thought
R5004	T7497	T7498	aux	would,induce
R5005	T7498	T7495	advcl	induce,predicted
R5006	T7499	T7500	amod	rapid,formation
R5007	T7500	T7498	dobj	formation,induce
R5008	T7649	T7646	pcomp	thought,Unlike
R5009	T7650	T7649	auxpass	is,thought
R5010	T7501	T7500	compound	plaque,formation
R5011	T7502	T7498	prep	in,induce
R5012	T7651	T7652	aux	to,occur
R5013	T7503	T7504	amod	untreated,animals
R5014	T7652	T7649	xcomp	occur,thought
R5015	T7504	T7502	pobj	animals,in
R5016	T7653	T7652	prep	in,occur
R5017	T7654	T7655	det	the,disease
R5018	T7505	T7477	punct	.,confirmed
R5019	T7655	T7653	pobj	disease,in
R5020	T7656	T7655	amod	human,disease
R5021	T7657	T7647	punct	", ",are
R5022	T7507	T7508	parataxis	Histological,characterization
R5023	T7658	T7659	det	the,plaques
R5024	T7659	T7647	nsubj	plaques,are
R5025	T7660	T7659	amod	first,plaques
R5026	T7508	T7509	nsubj	characterization,revealed
R5027	T7661	T7659	amod	visible,plaques
R5028	T7662	T7659	prep	in,plaques
R5029	T7663	T7664	det	the,mice
R5030	T7510	T7508	prep	of,characterization
R5031	T7664	T7662	pobj	mice,in
R5032	T7665	T7666	compound	tet,off
R5033	T7666	T7664	compound	off,mice
R5034	T7511	T7512	amod	double,mice
R5035	T7667	T7666	punct	-,off
R5036	T7668	T7664	compound	APP,mice
R5037	T7669	T7670	amod	fibrillar,cored
R5038	T7512	T7510	pobj	mice,of
R5039	T7670	T7672	amod	cored,deposits
R5040	T7671	T7670	punct	-,cored
R5041	T7672	T7647	attr	deposits,are
R5042	T7513	T7512	amod	transgenic,mice
R5043	T7673	T7647	punct	.,are
R5044	T7514	T7515	punct	(,APPswe
R5045	T7675	T7676	nsubj	We,noted
R5046	T7515	T7512	parataxis	APPswe,mice
R5047	T7677	T7676	aux	have,noted
R5048	T7516	T7515	nmod	CaMKIIα,APPswe
R5049	T7517	T7515	punct	-,APPswe
R5050	T7678	T7679	det	the,appearance
R5051	T7679	T7676	dobj	appearance,noted
R5052	T7518	T7515	nmod	tTA,APPswe
R5053	T7680	T7679	amod	same,appearance
R5054	T7681	T7679	amod	early,appearance
R5055	T7682	T7679	prep	of,appearance
R5056	T7683	T7684	amod	cored,deposits
R5057	T7684	T7682	pobj	deposits,of
R5058	T7519	T7515	punct	×,APPswe
R5059	T7685	T7679	prep	in,appearance
R5060	T7686	T7687	amod	other,lines
R5061	T7520	T7515	compound	tet,APPswe
R5062	T7687	T7685	pobj	lines,in
R5063	T7688	T7687	prep	of,lines
R5064	T7521	T7515	punct	-,APPswe
R5065	T7689	T7690	nmod	APP,mice
R5066	T7690	T7688	pobj	mice,of
R5067	T7691	T7690	amod	transgenic,mice
R5068	T7522	T7515	punct	/,APPswe
R5069	T7692	T7693	dep	that,harbor
R5070	T7693	T7687	relcl	harbor,lines
R5071	T7523	T7515	amod	ind,APPswe
R5072	T7694	T7695	det	the,mutation
R5073	T7695	T7693	dobj	mutation,harbor
R5074	T7696	T7695	amod	Swedish,mutation
R5075	T7524	T7515	punct	),APPswe
R5076	T7697	T7698	punct	[,27
R5077	T7698	T7676	parataxis	27,noted
R5078	T7525	T7526	amod	early,onset
R5079	T7699	T7698	punct	],27
R5080	T7700	T7676	punct	.,noted
R5081	T7526	T7528	compound	onset,formation
R5082	T7702	T7703	compound	Diffuse,plaques
R5083	T7703	T7704	nsubj	plaques,were
R5084	T7527	T7526	punct	-,onset
R5085	T7705	T7704	acomp	apparent,were
R5086	T7706	T7704	prep	in,were
R5087	T7528	T7509	dobj	formation,revealed
R5088	T7707	T7708	nummod	6,mo
R5089	T7708	T7710	npadvmod	mo,old
R5090	T7529	T7528	compound	amyloid,formation
R5091	T7709	T7708	punct	-,mo
R5092	T7710	T7712	amod	old,mice
R5093	T7711	T7710	punct	-,old
R5094	T7530	T7509	prep	in,revealed
R5095	T7712	T7706	pobj	mice,in
R5096	T7713	T7714	compound	tTA,APP
R5097	T7714	T7712	compound	APP,mice
R5098	T7531	T7532	det	all,lines
R5099	T7715	T7714	punct	/,APP
R5100	T7716	T7704	punct	", ",were
R5101	T7717	T7704	cc	and,were
R5102	T7532	T7530	pobj	lines,in
R5103	T7718	T7704	conj	became,were
R5104	T7719	T7720	advmod	relatively,abundant
R5105	T7533	T7532	nummod	four,lines
R5106	T7720	T7718	acomp	abundant,became
R5107	T7721	T7718	prep	by,became
R5108	T7722	T7723	nummod	9,mo
R5109	T7723	T7721	pobj	mo,by
R5110	T7724	T7723	prep	of,mo
R5111	T7725	T7724	pobj	age,of
R5112	T7534	T7532	amod	new,lines
R5113	T7726	T7704	punct	.,were
R5114	T7728	T7729	prep	At,were
R5115	T7535	T7509	punct	.,revealed
R5116	T7730	T7731	amod	older,ages
R5117	T7537	T7538	compound	Amyloid,plaques
R5118	T7731	T7728	pobj	ages,At
R5119	T7732	T7731	punct	(,ages
R5120	T7733	T7734	quantmod	9,12
R5121	T7538	T7539	nsubjpass	plaques,seen
R5122	T7734	T7736	nummod	12,mo
R5123	T7735	T7734	punct	–,12
R5124	T7736	T7731	appos	mo,ages
R5125	T7540	T7539	auxpass	were,seen
R5126	T7737	T7729	punct	),were
R5127	T7738	T7739	compound	amyloid,deposits
R5128	T7739	T7729	nsubj	deposits,were
R5129	T7541	T7539	prep	in,seen
R5130	T7740	T7729	acomp	visible,were
R5131	T7741	T7729	prep	in,were
R5132	T7742	T7743	det	the,thalamus
R5133	T7542	T7541	pobj	mice,in
R5134	T7743	T7741	pobj	thalamus,in
R5136	T7744	T7743	punct	", ",thalamus
R5137	T7745	T7746	dep	which,observed
R5138	T7746	T7743	relcl	observed,thalamus
R5139	T7747	T7746	aux	has,observed
R5140	T7748	T7746	advmod	also,observed
R5141	T7756	T7757	det	the,promoter
R5142	T7749	T7746	auxpass	been,observed
R5143	T7750	T7746	prep	in,observed
R5144	T7751	T7750	pobj	mice,in
R5145	T7757	T7755	pobj	promoter,via
R5146	T7752	T7751	acl	expressing,mice
R5147	T7753	T7754	compound	mutant,APP
R5148	T7754	T7752	dobj	APP,expressing
R5149	T7758	T7757	nmod	Thy,promoter
R5150	T7759	T7758	punct	-,Thy
R5151	T7760	T7758	nummod	1,Thy
R5152	T7761	T7729	punct	.,were
R5153	T7763	T7764	det	The,presence
R5154	T7861	T7859	amod	expressing,line
R5155	T7862	T7859	punct	(,line
R5156	T7863	T7859	appos	line,line
R5157	T7764	T7765	nsubjpass	presence,attributed
R5158	T7864	T7863	nummod	885,line
R5159	T7865	T7855	punct	),Animals
R5160	T7866	T7855	acl	maintained,Animals
R5161	T7867	T7866	prep	on,maintained
R5162	T7868	T7867	pobj	dox,on
R5163	T7869	T7866	prep	for,maintained
R5164	T7870	T7869	prep	up,for
R5165	T7871	T7870	prep	to,up
R5166	T7766	T7764	prep	of,presence
R5167	T7872	T7873	nummod	1,y
R5168	T7873	T7871	pobj	y,to
R5169	T7874	T7875	det	no,pathology
R5170	T7875	T7856	dobj	pathology,harbored
R5171	T7876	T7875	compound	amyloid,pathology
R5172	T7767	T7768	compound	amyloid,pathology
R5173	T7877	T7878	punct	(,shown
R5174	T7878	T7875	parataxis	shown,pathology
R5175	T7768	T7766	pobj	pathology,of
R5176	T7879	T7878	nsubj	data,shown
R5177	T7880	T7878	neg	not,shown
R5178	T7881	T7875	punct	),pathology
R5179	T7769	T7764	prep	in,presence
R5180	T7882	T7856	punct	", ",harbored
R5181	T7883	T7856	advcl	indicating,harbored
R5182	T7770	T7771	det	this,region
R5183	T7884	T7885	mark	that,provide
R5184	T7885	T7883	ccomp	provide,indicating
R5185	T7886	T7887	amod	residual,leakage
R5186	T7771	T7769	pobj	region,in
R5187	T7887	T7885	nsubj	leakage,provide
R5188	T7888	T7887	prep	of,leakage
R5189	T7772	T7765	aux	has,attributed
R5190	T7889	T7890	compound	transgene,expression
R5191	T7890	T7888	pobj	expression,of
R5192	T7891	T7887	prep	in,leakage
R5193	T7773	T7765	auxpass	been,attributed
R5194	T7892	T7893	det	the,presence
R5195	T7893	T7891	pobj	presence,in
R5196	T7894	T7893	prep	of,presence
R5197	T7774	T7765	prep	to,attributed
R5198	T7895	T7894	pobj	dox,of
R5199	T7896	T7885	aux	does,provide
R5200	T7897	T7885	neg	not,provide
R5201	T7775	T7776	amod	axonal,transport
R5202	T7898	T7899	amod	sufficient,peptide
R5203	T7899	T7885	dobj	peptide,provide
R5204	T7900	T7899	compound	Aβ,peptide
R5205	T7776	T7774	pobj	transport,to
R5206	T7901	T7902	aux	to,induce
R5207	T7902	T7899	acl	induce,peptide
R5208	T7903	T7904	compound	amyloid,formation
R5209	T7777	T7776	prep	of,transport
R5210	T7904	T7902	dobj	formation,induce
R5211	T7905	T7906	advmod	even,over
R5212	T7906	T7902	prep	over,induce
R5213	T7907	T7908	amod	long,periods
R5214	T7908	T7906	pobj	periods,over
R5215	T7778	T7779	compound	APP,Aβ
R5216	T7909	T7856	punct	.,harbored
R5217	T7911	T7912	aux	To,mimic
R5218	T7779	T7777	pobj	Aβ,of
R5219	T7912	T7913	advcl	mimic,raised
R5220	T7780	T7779	punct	/,Aβ
R5221	T7914	T7915	amod	therapeutic,intervention
R5222	T7915	T7912	dobj	intervention,mimic
R5223	T7916	T7912	prep	with,mimic
R5224	T7781	T7776	prep	to,transport
R5225	T7917	T7916	pobj	inhibitors,with
R5226	T7918	T7917	prep	of,inhibitors
R5227	T7782	T7783	det	the,terminals
R5228	T7919	T7920	compound	Aβ,production
R5229	T7920	T7918	pobj	production,of
R5230	T7921	T7913	punct	", ",raised
R5231	T7783	T7781	pobj	terminals,to
R5232	T7922	T7913	nsubj	we,raised
R5233	T7923	T7924	det	a,group
R5234	T7784	T7783	prep	of,terminals
R5235	T7924	T7913	dobj	group,raised
R5236	T7925	T7924	prep	of,group
R5237	T7785	T7786	amod	cortical,neurons
R5238	T7926	T7927	nummod	25,mice
R5239	T7927	T7925	pobj	mice,of
R5240	T7928	T7929	amod	double,transgenic
R5241	T7786	T7784	pobj	neurons,of
R5242	T7929	T7927	amod	transgenic,mice
R5243	T7930	T7931	punct	(,APP
R5244	T7787	T7783	prep	in,terminals
R5245	T7931	T7927	parataxis	APP,mice
R5246	T7932	T7931	nmod	CaMKIIα,APP
R5247	T7933	T7931	punct	-,APP
R5248	T7788	T7789	det	the,thalamus
R5249	T7934	T7931	nmod	tTA,APP
R5250	T7935	T7931	punct	×,APP
R5251	T7789	T7787	pobj	thalamus,in
R5252	T7936	T7931	appos	line,APP
R5253	T7790	T7791	punct	[,29
R5254	T7791	T7765	parataxis	29,attributed
R5255	T7937	T7936	nummod	107,line
R5256	T7938	T7931	punct	),APP
R5257	T7792	T7791	punct	],29
R5258	T7939	T7913	prep	on,raised
R5259	T7940	T7941	amod	normal,food
R5260	T7941	T7939	pobj	food,on
R5261	T7793	T7765	punct	.,attributed
R5262	T7942	T7913	prep	until,raised
R5263	T7943	T7944	nummod	6,mo
R5264	T7944	T7942	pobj	mo,until
R5265	T7945	T7944	prep	of,mo
R5266	T7795	T7796	advmod	Most,importantly
R5267	T7946	T7945	pobj	age,of
R5268	T7947	T7913	punct	", ",raised
R5269	T7948	T7949	advmod	when,knew
R5270	T7796	T7797	advmod	importantly,developed
R5271	T7949	T7913	advcl	knew,raised
R5272	T7950	T7949	nsubj	we,knew
R5273	T7798	T7797	punct	", ",developed
R5274	T7951	T7952	compound	amyloid,formation
R5275	T7952	T7953	nsubj	formation,was
R5276	T7953	T7949	advcl	was,knew
R5277	T7799	T7800	advmod	only,mice
R5278	T7954	T7953	advmod	already,was
R5279	T7955	T7956	advmod	well,underway
R5280	T7956	T7953	acomp	underway,was
R5281	T7800	T7797	nsubj	mice,developed
R5282	T7957	T7953	prep	in,was
R5283	T7958	T7959	det	the,brain
R5284	T7801	T7802	amod	double,transgenic
R5285	T7959	T7957	pobj	brain,in
R5286	T7960	T7913	punct	.,raised
R5287	T7802	T7800	amod	transgenic,mice
R5288	T7962	T7963	prep	At,switched
R5289	T7803	T7800	punct	", ",mice
R5290	T7964	T7965	nummod	6,mo
R5291	T7965	T7962	pobj	mo,At
R5292	T7804	T7800	acl	expressing,mice
R5293	T7966	T7963	punct	", ",switched
R5294	T7805	T7806	preconj	both,transgenes
R5295	T7806	T7804	dobj	transgenes,expressing
R5296	T7967	T7963	nsubjpass	half,switched
R5297	T7968	T7967	prep	of,half
R5298	T7807	T7806	det	the,transgenes
R5299	T7969	T7970	det	the,animals
R5300	T7970	T7968	pobj	animals,of
R5301	T7808	T7806	nmod	tTA,transgenes
R5302	T7971	T7963	auxpass	were,switched
R5303	T7809	T7808	cc	and,tTA
R5304	T7972	T7963	prep	from,switched
R5305	T7973	T7974	amod	normal,chow
R5306	T7974	T7972	pobj	chow,from
R5307	T7810	T7808	conj	APP,tTA
R5308	T7975	T7963	prep	to,switched
R5309	T7976	T7975	pobj	food,to
R5310	T7811	T7797	punct	", ",developed
R5311	T7977	T7976	acl	containing,food
R5312	T7978	T7977	dobj	dox,containing
R5313	T7979	T7977	prep	at,containing
R5314	T7980	T7981	nummod	200,mg
R5315	T7981	T7979	pobj	mg,at
R5316	T7812	T7813	compound	amyloid,lesions
R5317	T7982	T7983	punct	/,kg
R5318	T7983	T7981	prep	kg,mg
R5319	T7984	T7985	mark	until,sacrificed
R5320	T7813	T7797	dobj	lesions,developed
R5321	T7985	T7963	advcl	sacrificed,switched
R5322	T7986	T7985	nsubjpass	they,sacrificed
R5323	T7987	T7985	auxpass	were,sacrificed
R5324	T7814	T7797	punct	.,developed
R5325	T7988	T7985	prep	at,sacrificed
R5326	T7989	T7990	nummod	9,mo
R5327	T7816	T7817	amod	Single,transgenic
R5328	T7990	T7988	pobj	mo,at
R5329	T7991	T7989	cc	or,9
R5330	T7992	T7989	conj	12,9
R5331	T7993	T7990	prep	of,mo
R5332	T7817	T7818	amod	transgenic,mice
R5333	T7994	T7993	pobj	age,of
R5334	T7995	T7963	punct	.,switched
R5335	T7818	T7819	nsubj	mice,showed
R5336	T7997	T7998	det	The,animals
R5337	T7998	T8001	nsubjpass	animals,kept
R5338	T7820	T7821	quantmod	up,15
R5339	T7999	T7998	amod	remaining,animals
R5340	T8000	T7998	compound	control,animals
R5341	T7821	T7823	nummod	15,mo
R5342	T8002	T8001	auxpass	were,kept
R5343	T8003	T8001	prep	on,kept
R5344	T8004	T8005	amod	standard,chow
R5345	T7822	T7821	quantmod	to,15
R5346	T8005	T8003	pobj	chow,on
R5347	T8006	T8007	punct	(,untreated
R5348	T7823	T7818	npadvmod	mo,mice
R5349	T8007	T8001	parataxis	untreated,kept
R5350	T8008	T8007	punct	),untreated
R5351	T8009	T8001	punct	.,kept
R5352	T7824	T7823	prep	of,mo
R5353	T8011	T8012	prep	In,created
R5354	T7825	T7824	pobj	age,of
R5355	T8013	T8011	pobj	all,In
R5356	T8014	T8012	punct	", ",created
R5357	T7826	T7827	det	no,sign
R5358	T8015	T8016	nummod	four,cohorts
R5359	T7827	T7819	dobj	sign,showed
R5360	T8016	T8012	nsubjpass	cohorts,created
R5361	T7828	T7827	prep	of,sign
R5362	T8017	T8012	auxpass	were,created
R5363	T8018	T8012	punct	: ,created
R5364	T7829	T7828	pobj	pathology,of
R5365	T8019	T8020	nummod	6,mo
R5366	T8020	T8012	dobj	mo,created
R5367	T8021	T8020	amod	untreated,mo
R5368	T8022	T8023	punct	(,7
R5369	T7830	T7831	punct	(,S3
R5370	T8023	T8020	parataxis	7,mo
R5371	T7831	T7819	parataxis	S3,showed
R5372	T8024	T8023	nsubj	n,7
R5373	T8025	T8023	punct	=,7
R5374	T7832	T7831	compound	Figure,S3
R5375	T8026	T8023	punct	),7
R5376	T8027	T8020	punct	", ",mo
R5377	T7833	T7831	punct	),S3
R5378	T8028	T8029	nummod	9,mo
R5379	T8029	T8020	conj	mo,mo
R5380	T8030	T8029	amod	untreated,mo
R5381	T7834	T7819	punct	.,showed
R5382	T8031	T8032	punct	(,5
R5383	T8032	T8029	parataxis	5,mo
R5384	T7836	T7837	advmod	Similarly,prevented
R5385	T8033	T8032	nsubj	n,5
R5386	T8034	T8032	punct	=,5
R5387	T8035	T8032	punct	),5
R5388	T7838	T7837	punct	", ",prevented
R5389	T8036	T8029	punct	", ",mo
R5390	T8037	T8038	nummod	6,mo
R5391	T8038	T8029	conj	mo,mo
R5392	T8039	T8038	punct	+,mo
R5393	T7839	T7840	compound	amyloid,pathology
R5394	T8040	T8041	nummod	3,mo
R5395	T7840	T7837	nsubjpass	pathology,prevented
R5396	T7841	T7837	aux	can,prevented
R5397	T8041	T8038	appos	mo,mo
R5398	T7842	T7837	auxpass	be,prevented
R5399	T8042	T8038	amod	treated,mo
R5400	T8043	T8044	punct	(,8
R5401	T7843	T7837	advmod	completely,prevented
R5402	T8044	T8038	parataxis	8,mo
R5403	T8045	T8044	nsubj	n,8
R5404	T7844	T7837	prep	in,prevented
R5405	T8046	T8044	punct	=,8
R5406	T8047	T8044	punct	),8
R5407	T8048	T8038	punct	", ",mo
R5408	T7845	T7846	amod	double,transgenic
R5409	T8049	T8038	cc	and,mo
R5410	T8050	T8051	nummod	6,mo
R5411	T8051	T8038	conj	mo,mo
R5412	T7846	T7847	amod	transgenic,animals
R5413	T8052	T8051	punct	+,mo
R5414	T8053	T8054	nummod	6,mo
R5415	T7847	T7844	pobj	animals,in
R5419	T8057	T8051	parataxis	5,mo
R5420	T8058	T8057	nsubj	n,5
R5421	T7848	T7847	acl	born,animals
R5422	T8059	T8057	punct	=,5
R5423	T8060	T8057	punct	),5
R5424	T8061	T8012	punct	.,created
R5425	T7849	T7848	cc	and,born
R5426	T8063	T8064	amod	Full,suppression
R5427	T7850	T7848	conj	raised,born
R5428	T8064	T8065	nsubjpass	suppression,confirmed
R5429	T8066	T8067	punct	(,%
R5430	T7851	T7850	prep	on,raised
R5431	T8067	T8064	parataxis	%,suppression
R5432	T8068	T8069	punct	>,95
R5433	T8069	T8067	nummod	95,%
R5434	T7852	T7851	pobj	dox,on
R5435	T8070	T8067	punct	),%
R5436	T8071	T8064	prep	of,suppression
R5437	T7853	T7837	punct	.,prevented
R5438	T7855	T7856	nsubj	Animals,harbored
R5439	T7857	T7855	prep	from,Animals
R5440	T8072	T8073	amod	transgenic,levels
R5441	T8073	T8071	pobj	levels,of
R5442	T8074	T8075	compound	APPswe,ind
R5443	T7858	T7859	poss	our,line
R5444	T8075	T8073	compound	ind,levels
R5445	T8076	T8075	punct	/,ind
R5446	T8077	T8073	prep	in,levels
R5447	T7859	T7857	pobj	line,from
R5448	T8078	T8079	det	the,animals
R5449	T8079	T8077	pobj	animals,in
R5450	T7860	T7861	amod	highest,expressing
R5451	T8080	T8081	npadvmod	dox,treated
R5452	T8081	T8079	amod	treated,animals
R5453	T8178	T8176	pobj	ind,of
R5454	T8082	T8081	punct	-,treated
R5455	T8083	T8065	auxpass	was,confirmed
R5456	T8084	T8065	prep	by,confirmed
R5457	T8085	T8084	pobj	immunoblot,by
R5458	T8086	T8087	punct	(,Figure
R5459	T8179	T8178	punct	/,ind
R5460	T8087	T8065	parataxis	Figure,confirmed
R5461	T8088	T8087	nummod	3,Figure
R5462	T8089	T8087	punct	),Figure
R5463	T8180	T8171	cc	and,life
R5464	T8090	T8065	punct	.,confirmed
R5465	T8181	T8182	poss	its,fragments
R5466	T8092	T8093	aux	To,ensure
R5467	T8093	T8094	advcl	ensure,treated
R5468	T8182	T8171	conj	fragments,life
R5469	T8095	T8096	mark	that,suppressed
R5470	T8096	T8093	ccomp	suppressed,ensure
R5471	T8097	T8098	det	the,transgene
R5472	T8098	T8096	nsubjpass	transgene,suppressed
R5473	T8099	T8096	aux	could,suppressed
R5474	T8183	T8182	amod	processed,fragments
R5475	T8100	T8096	auxpass	be,suppressed
R5476	T8101	T8102	advmod	as,rapidly
R5477	T8102	T8096	advmod	rapidly,suppressed
R5478	T8184	T8185	npadvmod	C,terminal
R5479	T8103	T8096	prep	in,suppressed
R5480	T8104	T8105	nummod	6,mo
R5481	T8105	T8107	npadvmod	mo,old
R5482	T8185	T8182	amod	terminal,fragments
R5483	T8106	T8105	punct	-,mo
R5484	T8107	T8109	amod	old,mice
R5485	T8108	T8107	punct	-,old
R5486	T8186	T8185	punct	-,terminal
R5487	T8187	T8188	advmod	relatively,short
R5488	T8188	T8169	acomp	short,are
R5489	T8109	T8103	pobj	mice,in
R5490	T8110	T8109	prep	with,mice
R5491	T8111	T8112	amod	fulminant,pathology
R5492	T8189	T8190	punct	(,3D
R5493	T8112	T8110	pobj	pathology,with
R5494	T8113	T8114	mark	as,can
R5495	T8190	T8144	parataxis	3D,suppressed
R5496	T8114	T8096	advcl	can,suppressed
R5497	T8115	T8114	nsubj	it,can
R5498	T8191	T8190	compound	Figure,3D
R5499	T8116	T8114	prep	in,can
R5500	T8117	T8118	amod	young,animals
R5501	T8192	T8190	punct	),3D
R5502	T8118	T8116	pobj	animals,in
R5503	T8119	T8118	punct	", ",animals
R5504	T8120	T8118	amod	predeposit,animals
R5505	T8121	T8094	punct	", ",treated
R5506	T8122	T8094	nsubj	we,treated
R5507	T8123	T8124	det	an,set
R5508	T8193	T8144	punct	.,suppressed
R5509	T8124	T8094	dobj	set,treated
R5510	T8125	T8124	amod	additional,set
R5511	T8126	T8124	prep	of,set
R5512	T8195	T8196	compound	Tissue,sections
R5513	T8127	T8128	nummod	6,mo
R5514	T8128	T8130	npadvmod	mo,old
R5515	T8129	T8128	punct	-,mo
R5516	T8130	T8132	amod	old,animals
R5517	T8196	T8197	nsubjpass	sections,stained
R5518	T8131	T8130	punct	-,old
R5519	T8132	T8126	pobj	animals,of
R5520	T8198	T8196	prep	from,sections
R5521	T8133	T8132	prep	with,animals
R5522	T8134	T8133	pobj	dox,with
R5523	T8135	T8124	prep	for,set
R5524	T8136	T8137	nummod	1,wk
R5525	T8137	T8135	pobj	wk,for
R5526	T8138	T8139	amod	prior,to
R5527	T8139	T8124	prep	to,set
R5528	T8199	T8200	det	each,animal
R5529	T8140	T8139	pobj	harvest,to
R5530	T8141	T8094	punct	.,treated
R5531	T8200	T8198	pobj	animal,from
R5532	T8143	T8144	advmod	Importantly,suppressed
R5533	T8201	T8200	prep	in,animal
R5534	T8145	T8144	punct	", ",suppressed
R5535	T8146	T8147	preconj	both,ind
R5536	T8147	T8150	npadvmod	ind,terminal
R5537	T8202	T8203	det	the,groups
R5538	T8148	T8147	compound	APPswe,ind
R5539	T8149	T8147	punct	/,ind
R5540	T8150	T8156	amod	terminal,levels
R5541	T8203	T8201	pobj	groups,in
R5542	T8151	T8147	cc	and,ind
R5543	T8152	T8153	compound	APP,C
R5544	T8153	T8147	conj	C,ind
R5545	T8154	T8153	punct	–,C
R5546	T8204	T8203	nummod	four,groups
R5547	T8155	T8150	punct	-,terminal
R5548	T8156	T8144	nsubjpass	levels,suppressed
R5549	T8157	T8156	compound	fragment,levels
R5550	T8205	T8203	compound	treatment,groups
R5551	T8158	T8144	auxpass	were,suppressed
R5552	T8159	T8144	advmod	fully,suppressed
R5553	T8206	T8197	auxpass	were,stained
R5554	T8160	T8144	prep	after,suppressed
R5555	T8161	T8162	advmod	only,wk
R5556	T8162	T8160	pobj	wk,after
R5557	T8207	T8197	prep	for,stained
R5558	T8163	T8162	nummod	1,wk
R5559	T8164	T8162	prep	of,wk
R5560	T8208	T8209	compound	amyloid,pathology
R5561	T8165	T8164	pobj	treatment,of
R5562	T8166	T8144	punct	", ",suppressed
R5563	T8167	T8144	advcl	indicating,suppressed
R5564	T8209	T8207	pobj	pathology,for
R5565	T8168	T8169	mark	that,are
R5566	T8169	T8167	ccomp	are,indicating
R5567	T8170	T8171	det	the,life
R5568	T8210	T8197	agent	by,stained
R5569	T8171	T8169	nsubj	life,are
R5570	T8172	T8173	advmod	in,vivo
R5571	T8211	T8212	compound	Hirano,silver
R5572	T8173	T8171	amod	vivo,life
R5573	T8174	T8171	compound	half,life
R5574	T8175	T8171	punct	-,life
R5575	T8176	T8171	prep	of,life
R5576	T8177	T8178	compound	APPswe,ind
R5577	T8212	T8210	pobj	silver,by
R5578	T8213	T8212	punct	", ",silver
R5579	T8214	T8215	compound	Campbell,Switzer
R5580	T8215	T8217	compound	Switzer,silver
R5581	T8216	T8215	punct	-,Switzer
R5582	T8284	T8283	prep	of,that
R5583	T8285	T8286	det	the,cohort
R5584	T8217	T8212	conj	silver,silver
R5585	T8286	T8284	pobj	cohort,of
R5586	T8287	T8288	nummod	6,mo
R5587	T8218	T8217	punct	", ",silver
R5588	T8288	T8289	npadvmod	mo,untreated
R5589	T8289	T8286	amod	untreated,cohort
R5590	T8290	T8256	punct	", ",resembled
R5591	T8219	T8220	compound	thioflavin,S
R5592	T8291	T8256	prep	despite,resembled
R5593	T8292	T8293	det	the,difference
R5594	T8220	T8217	conj	S,silver
R5595	T8293	T8291	pobj	difference,despite
R5596	T8294	T8293	amod	significant,difference
R5597	T8295	T8293	compound	age,difference
R5598	T8221	T8220	punct	-,S
R5599	T8296	T8293	prep	between,difference
R5600	T8297	T8298	det	the,groups
R5601	T8222	T8220	punct	", ",S
R5602	T8298	T8296	pobj	groups,between
R5603	T8299	T8298	amod	treated,groups
R5604	T8300	T8299	cc	and,treated
R5605	T8223	T8220	cc	and,S
R5606	T8301	T8299	conj	untreated,treated
R5607	T8302	T8303	punct	(,Figures
R5608	T8303	T8256	parataxis	Figures,resembled
R5609	T8224	T8225	compound	Aβ,immunohistochemistry
R5610	T8304	T8303	nummod	4,Figures
R5611	T8305	T8303	cc	and,Figures
R5612	T8306	T8307	compound	Figure,S3
R5613	T8225	T8220	conj	immunohistochemistry,S
R5614	T8307	T8303	conj	S3,Figures
R5615	T8308	T8303	punct	),Figures
R5616	T8226	T8197	punct	.,stained
R5617	T8309	T8256	punct	.,resembled
R5618	T8311	T8312	advmod	Well,formed
R5619	T8228	T8229	mark	As,expected
R5620	T8312	T8314	amod	formed,plaques
R5621	T8313	T8312	punct	-,formed
R5622	T8314	T8315	nsubj	plaques,remained
R5623	T8229	T8230	advcl	expected,displayed
R5624	T8316	T8315	prep	in,remained
R5625	T8317	T8318	det	the,animals
R5626	T8318	T8316	pobj	animals,in
R5627	T8319	T8318	amod	treated,animals
R5628	T8320	T8315	prep	after,remained
R5629	T8231	T8230	punct	", ",displayed
R5630	T8321	T8322	nummod	6,mo
R5631	T8322	T8320	pobj	mo,after
R5632	T8323	T8322	prep	of,mo
R5633	T8324	T8325	compound	transgene,suppression
R5634	T8232	T8233	det	the,cohort
R5635	T8325	T8323	pobj	suppression,of
R5636	T8326	T8315	punct	", ",remained
R5637	T8233	T8230	nsubj	cohort,displayed
R5638	T8327	T8328	advmod	even,given
R5639	T8328	T8315	advcl	given,remained
R5640	T8329	T8328	mark	though,given
R5641	T8234	T8235	nummod	6,mo
R5642	T8330	T8331	advmod	as,much
R5643	T8331	T8332	amod	much,time
R5644	T8332	T8328	nsubjpass	time,given
R5645	T8235	T8233	nmod	mo,cohort
R5646	T8333	T8328	auxpass	was,given
R5647	T8334	T8335	aux	to,clear
R5648	T8335	T8328	advcl	clear,given
R5649	T8236	T8233	amod	untreated,cohort
R5650	T8336	T8337	det	the,lesions
R5651	T8337	T8335	dobj	lesions,clear
R5652	T8338	T8339	mark	as,taken
R5653	T8237	T8238	amod	moderate,pathology
R5654	T8339	T8328	advcl	taken,given
R5655	T8340	T8339	nsubj	they,taken
R5656	T8238	T8230	dobj	pathology,displayed
R5657	T8341	T8339	aux	had,taken
R5658	T8342	T8343	aux	to,form
R5659	T8343	T8339	advcl	form,taken
R5660	T8239	T8238	compound	amyloid,pathology
R5661	T8344	T8315	punct	.,remained
R5662	T8346	T8347	advmod	Moreover,remained
R5663	T8240	T8230	punct	", ",displayed
R5664	T8348	T8347	punct	", ",remained
R5665	T8241	T8230	cc	and,displayed
R5666	T8349	T8350	det	both,types
R5667	T8350	T8347	nsubj	types,remained
R5668	T8351	T8350	prep	of,types
R5669	T8242	T8243	det	the,cohort
R5670	T8352	T8351	pobj	amyloid,of
R5671	T8353	T8350	punct	", ",types
R5672	T8354	T8350	amod	diffuse,types
R5673	T8243	T8247	nsubj	cohort,progressed
R5674	T8355	T8354	cc	and,diffuse
R5675	T8356	T8354	conj	fibrillar,diffuse
R5676	T8244	T8245	nummod	9,mo
R5677	T8357	T8347	punct	", ",remained
R5678	T8358	T8347	acomp	intact,remained
R5679	T8359	T8347	prep	throughout,remained
R5680	T8360	T8359	pobj	treatment,throughout
R5681	T8361	T8347	punct	.,remained
R5682	T8245	T8243	nmod	mo,cohort
R5683	T8363	T8364	advcl	Using,found
R5684	T8365	T8366	det	the,stain
R5685	T8246	T8243	amod	untreated,cohort
R5686	T8247	T8230	conj	progressed,displayed
R5687	T8248	T8247	prep	to,progressed
R5688	T8366	T8363	dobj	stain,Using
R5689	T8367	T8368	compound	Campbell,Switzer
R5690	T8249	T8250	det	a,burden
R5691	T8368	T8366	compound	Switzer,stain
R5692	T8369	T8368	punct	-,Switzer
R5693	T8370	T8366	compound	silver,stain
R5694	T8250	T8248	pobj	burden,to
R5695	T8371	T8372	aux	to,distinguish
R5696	T8372	T8363	advcl	distinguish,Using
R5697	T8251	T8250	amod	severe,burden
R5698	T8373	T8374	amod	different,forms
R5699	T8374	T8372	dobj	forms,distinguish
R5700	T8252	T8250	compound	amyloid,burden
R5701	T8375	T8374	prep	of,forms
R5702	T8376	T8375	pobj	amyloid,of
R5703	T8377	T8364	punct	", ",found
R5704	T8253	T8247	punct	.,progressed
R5705	T8378	T8364	nsubj	we,found
R5706	T8379	T8380	amod	diffuse,plaques
R5707	T8255	T8256	prep	In,resembled
R5708	T8380	T8381	nsubj	plaques,were
R5709	T8381	T8364	advcl	were,found
R5710	T8382	T8383	advmod	as,persistent
R5711	T8383	T8381	acomp	persistent,were
R5712	T8257	T8255	pobj	contrast,In
R5713	T8384	T8383	prep	as,persistent
R5714	T8385	T8386	amod	cored,deposits
R5715	T8386	T8384	pobj	deposits,as
R5716	T8258	T8256	punct	", ",resembled
R5717	T8387	T8388	punct	(,S4
R5718	T8388	T8364	parataxis	S4,found
R5719	T8389	T8388	compound	Figure,S4
R5720	T8259	T8260	det	the,extent
R5721	T8260	T8256	nsubj	extent,resembled
R5722	T8261	T8260	prep	of,extent
R5723	T8390	T8388	punct	),S4
R5724	T8262	T8263	compound	amyloid,pathology
R5725	T8391	T8364	punct	.,found
R5726	T8263	T8261	pobj	pathology,of
R5727	T8393	T8394	nsubj	It,was
R5728	T8395	T8394	advmod	nevertheless,was
R5729	T8396	T8394	acomp	clear,was
R5730	T8397	T8398	mark	that,halted
R5731	T8398	T8394	ccomp	halted,was
R5732	T8264	T8260	prep	in,extent
R5733	T8399	T8400	npadvmod	dox,induced
R5734	T8400	T8402	amod	induced,suppression
R5735	T8401	T8400	punct	-,induced
R5736	T8265	T8264	pobj	mice,in
R5737	T8402	T8398	nsubj	suppression,halted
R5738	T8403	T8402	prep	of,suppression
R5739	T8266	T8265	prep	from,mice
R5740	T8404	T8405	amod	transgenic,APP
R5741	T8405	T8403	pobj	APP,of
R5742	T8406	T8398	aux	had,halted
R5743	T8267	T8268	det	the,cohorts
R5744	T8407	T8398	advmod	completely,halted
R5745	T8408	T8409	det	the,progression
R5746	T8268	T8266	pobj	cohorts,from
R5747	T8409	T8398	dobj	progression,halted
R5748	T8410	T8409	prep	of,progression
R5749	T8411	T8410	pobj	pathology,of
R5750	T8269	T8270	nummod	6,mo
R5751	T8412	T8394	punct	.,was
R5752	T8270	T8271	npadvmod	mo,treated
R5753	T8414	T8415	aux	To,confirm
R5754	T8415	T8416	advcl	confirm,repeated
R5755	T8271	T8268	amod	treated,cohorts
R5756	T8417	T8418	mark	that,was
R5757	T8418	T8415	ccomp	was,confirm
R5758	T8272	T8270	punct	+,mo
R5759	T8419	T8420	det	the,arrest
R5760	T8420	T8418	nsubj	arrest,was
R5761	T8421	T8420	prep	of,arrest
R5762	T8273	T8274	nummod	3,mo
R5763	T8422	T8421	pobj	plaques,of
R5764	T8423	T8420	prep	without,arrest
R5765	T8274	T8270	appos	mo,mo
R5766	T8424	T8425	det	any,sign
R5767	T8425	T8423	pobj	sign,without
R5768	T8426	T8425	prep	of,sign
R5769	T8275	T8271	cc	or,treated
R5770	T8427	T8426	pobj	clearance,of
R5771	T8428	T8418	neg	not,was
R5772	T8276	T8277	nummod	6,mo
R5773	T8277	T8278	npadvmod	mo,treated
R5774	T8278	T8271	conj	treated,treated
R5775	T8429	T8418	acomp	unique,was
R5776	T8430	T8429	prep	to,unique
R5777	T8279	T8277	punct	+,mo
R5778	T8431	T8432	det	the,mice
R5779	T8432	T8430	pobj	mice,to
R5780	T8280	T8281	nummod	6,mo
R5781	T8433	T8432	nmod	line,mice
R5782	T8434	T8433	nummod	107,line
R5783	T8435	T8416	punct	", ",repeated
R5784	T8436	T8416	nsubj	we,repeated
R5785	T8437	T8438	det	the,experiment
R5786	T8438	T8416	dobj	experiment,repeated
R5787	T8281	T8277	appos	mo,mo
R5788	T8439	T8440	compound	dox,suppression
R5789	T8440	T8438	compound	suppression,experiment
R5790	T8282	T8256	advmod	closely,resembled
R5791	T8441	T8440	punct	-,suppression
R5792	T8442	T8416	prep	in,repeated
R5793	T8443	T8444	det	a,line
R5794	T8283	T8256	dobj	that,resembled
R5795	T8444	T8442	pobj	line,in
R5796	T8445	T8444	amod	second,line
R5797	T8446	T8444	prep	of,line
R5798	T8496	T8497	punct	(,8
R5799	T8447	T8448	compound	tet,off
R5800	T8448	T8450	compound	off,mice
R5801	T8449	T8448	punct	-,off
R5802	T8497	T8488	parataxis	8,harvested
R5803	T8498	T8499	nummod	6,mo
R5804	T8450	T8446	pobj	mice,of
R5805	T8451	T8450	compound	APP,mice
R5806	T8499	T8500	npadvmod	mo,untreated
R5807	T8452	T8453	punct	(,line
R5808	T8453	T8416	parataxis	line,repeated
R5809	T8500	T8501	amod	untreated,8
R5810	T8454	T8455	nmod	CaMKIIα,ind
R5811	T8455	T8453	compound	ind,line
R5812	T8501	T8497	dep	8,8
R5813	T8456	T8455	punct	-,ind
R5814	T8457	T8455	nmod	tTA,ind
R5815	T8458	T8455	punct	×,ind
R5816	T8502	T8501	punct	", ",8
R5817	T8459	T8455	compound	tet,ind
R5818	T8460	T8455	punct	-,ind
R5819	T8503	T8501	nsubj	n,8
R5820	T8461	T8455	compound	APPswe,ind
R5821	T8462	T8455	punct	/,ind
R5822	T8463	T8453	nummod	18,line
R5823	T8504	T8501	punct	=,8
R5824	T8464	T8453	punct	;,line
R5825	T8465	T8466	nsubj	n,22
R5826	T8505	T8497	punct	;,8
R5827	T8466	T8453	ccomp	22,line
R5828	T8467	T8466	punct	=,22
R5829	T8468	T8453	punct	),line
R5830	T8506	T8507	nummod	9,mo
R5831	T8469	T8416	punct	.,repeated
R5832	T8471	T8472	advmod	Again,begun
R5833	T8473	T8472	punct	", ",begun
R5834	T8507	T8508	npadvmod	mo,untreated
R5835	T8474	T8475	amod	long,term
R5836	T8475	T8477	compound	term,treatment
R5837	T8476	T8475	punct	-,term
R5838	T8508	T8509	amod	untreated,6
R5839	T8477	T8472	nsubjpass	treatment,begun
R5840	T8478	T8477	compound	dox,treatment
R5841	T8509	T8497	dep	6,8
R5842	T8479	T8472	auxpass	was,begun
R5843	T8480	T8472	prep	at,begun
R5844	T8481	T8482	nummod	6,mo
R5845	T8510	T8509	punct	", ",6
R5846	T8482	T8480	pobj	mo,at
R5847	T8483	T8482	prep	of,mo
R5848	T8511	T8509	nsubj	n,6
R5849	T8484	T8483	pobj	age,of
R5850	T8485	T8472	punct	", ",begun
R5851	T8486	T8472	cc	and,begun
R5852	T8512	T8509	punct	=,6
R5853	T8487	T8488	nsubjpass	mice,harvested
R5854	T8488	T8472	conj	harvested,begun
R5855	T8489	T8488	auxpass	were,harvested
R5856	T8513	T8497	punct	;,8
R5857	T8490	T8488	prep	after,harvested
R5858	T8491	T8492	nummod	3,mo
R5859	T8514	T8515	nummod	6,mo
R5860	T8492	T8490	pobj	mo,after
R5861	T8493	T8492	prep	of,mo
R5862	T8494	T8495	compound	transgene,suppression
R5863	T8515	T8519	npadvmod	mo,treated
R5864	T8495	T8493	pobj	suppression,of
R5865	T8516	T8515	nmod	mo,mo
R5866	T8517	T8515	punct	+,mo
R5867	T8518	T8515	nummod	3,mo
R5868	T8519	T8497	amod	treated,8
R5869	T8603	T8602	aux	could,found
R5870	T8520	T8497	punct	", ",8
R5871	T8604	T8602	advmod	still,found
R5872	T8605	T8602	auxpass	be,found
R5873	T8606	T8602	prep	in,found
R5874	T8521	T8497	nsubj	n,8
R5875	T8607	T8606	pobj	each,in
R5876	T8608	T8607	prep	of,each
R5877	T8522	T8497	punct	=,8
R5878	T8609	T8610	det	the,animals
R5879	T8610	T8608	pobj	animals,of
R5880	T8611	T8612	npadvmod	dox,treated
R5881	T8523	T8497	punct	),8
R5882	T8612	T8610	amod	treated,animals
R5883	T8613	T8612	punct	-,treated
R5884	T8614	T8602	punct	.,found
R5885	T8616	T8617	nsubj	We,measured
R5886	T8524	T8488	punct	.,harvested
R5887	T8618	T8617	advmod	biochemically,measured
R5888	T8619	T8620	det	the,amount
R5889	T8526	T8527	nsubj	Immunoblotting,confirmed
R5890	T8620	T8617	dobj	amount,measured
R5891	T8621	T8620	prep	of,amount
R5892	T8622	T8623	amod	aggregated,Aβ
R5893	T8623	T8621	pobj	Aβ,of
R5894	T8528	T8526	prep	for,Immunoblotting
R5895	T8624	T8617	prep	in,measured
R5896	T8529	T8528	pobj	APP,for
R5897	T8530	T8531	amod	full,suppression
R5898	T8625	T8626	det	the,brains
R5899	T8626	T8624	pobj	brains,in
R5900	T8531	T8527	dobj	suppression,confirmed
R5901	T8627	T8626	prep	of,brains
R5902	T8628	T8629	poss	our,mice
R5903	T8629	T8627	pobj	mice,of
R5904	T8532	T8531	compound	transgene,suppression
R5905	T8630	T8617	prep	before,measured
R5906	T8631	T8630	cc	and,before
R5907	T8533	T8527	prep	in,confirmed
R5908	T8632	T8630	conj	after,before
R5909	T8633	T8634	compound	transgene,suppression
R5910	T8534	T8535	det	the,animals
R5911	T8634	T8632	pobj	suppression,after
R5912	T8635	T8617	advcl	using,measured
R5913	T8636	T8637	compound	filter,trap
R5914	T8535	T8533	pobj	animals,in
R5915	T8637	T8638	compound	trap,analysis
R5916	T8638	T8635	dobj	analysis,using
R5917	T8536	T8535	amod	treated,animals
R5918	T8639	T8638	prep	of,analysis
R5919	T8640	T8641	amod	cortical,tissue
R5920	T8641	T8639	pobj	tissue,of
R5921	T8537	T8538	punct	(,S5
R5922	T8642	T8641	prep	from,tissue
R5923	T8643	T8644	det	each,animal
R5924	T8644	T8642	pobj	animal,from
R5925	T8538	T8527	parataxis	S5,confirmed
R5926	T8645	T8617	punct	.,measured
R5927	T8539	T8538	compound	Figure,S5
R5928	T8647	T8648	prep	In,passed
R5929	T8648	T8658	ccomp	passed,become
R5930	T8649	T8650	det	this,assay
R5931	T8540	T8538	punct	),S5
R5932	T8650	T8647	pobj	assay,In
R5933	T8651	T8648	punct	", ",passed
R5934	T8541	T8527	punct	.,confirmed
R5935	T8652	T8653	amod	serial,dilutions
R5936	T8653	T8648	nsubjpass	dilutions,passed
R5937	T8654	T8653	prep	of,dilutions
R5938	T8655	T8656	compound	protein,homogenate
R5939	T8656	T8654	pobj	homogenate,of
R5940	T8543	T8544	prep	As,worsened
R5941	T8657	T8648	auxpass	are,passed
R5942	T8659	T8648	prep	through,passed
R5943	T8660	T8661	det	a,filter
R5944	T8545	T8543	prep	in,As
R5945	T8661	T8659	pobj	filter,through
R5946	T8662	T8661	compound	cellulose,filter
R5947	T8663	T8661	compound	acetate,filter
R5948	T8664	T8658	punct	;,become
R5949	T8546	T8547	det	the,mice
R5950	T8665	T8658	nsubj	particles,become
R5951	T8666	T8665	amod	larger,particles
R5952	T8547	T8545	pobj	mice,in
R5953	T8667	T8666	prep	than,larger
R5954	T8668	T8669	det	the,size
R5955	T8548	T8547	nmod	line,mice
R5956	T8669	T8667	pobj	size,than
R5957	T8670	T8669	compound	pore,size
R5958	T8549	T8548	nummod	107,line
R5959	T8671	T8669	prep	of,size
R5960	T8672	T8673	det	the,filter
R5961	T8673	T8671	pobj	filter,of
R5962	T8550	T8547	acl	described,mice
R5963	T8674	T8658	acomp	trapped,become
R5964	T8675	T8674	prep	in,trapped
R5965	T8551	T8550	advmod	above,described
R5966	T8676	T8677	det	the,membrane
R5967	T8677	T8675	pobj	membrane,in
R5968	T8552	T8544	punct	", ",worsened
R5969	T8678	T8658	cc	and,become
R5970	T8679	T8680	auxpass	are,revealed
R5971	T8553	T8554	compound	amyloid,burden
R5972	T8680	T8658	conj	revealed,become
R5973	T8681	T8680	prep	by,revealed
R5974	T8682	T8681	pobj	immunoblotting,by
R5975	T8554	T8544	nsubj	burden,worsened
R5976	T8683	T8684	punct	[,22
R5977	T8684	T8680	parataxis	22,revealed
R5978	T8555	T8544	advmod	substantially,worsened
R5979	T8685	T8684	punct	],22
R5980	T8686	T8658	punct	.,become
R5981	T8556	T8544	prep	in,worsened
R5982	T8688	T8689	advcl	Consistent,had
R5983	T8557	T8558	det	the,mice
R5984	T8690	T8688	prep	with,Consistent
R5985	T8691	T8692	poss	our,analysis
R5986	T8558	T8556	pobj	mice,in
R5987	T8692	T8690	pobj	analysis,with
R5988	T8693	T8692	amod	visual,analysis
R5989	T8694	T8692	prep	of,analysis
R5990	T8695	T8696	det	the,sections
R5991	T8696	T8694	pobj	sections,of
R5992	T8697	T8696	amod	histological,sections
R5993	T8559	T8558	amod	untreated,mice
R5994	T8698	T8689	punct	", ",had
R5995	T8699	T8700	nmod	line,mice
R5996	T8560	T8558	prep	between,mice
R5997	T8700	T8689	nsubj	mice,had
R5998	T8701	T8699	nummod	107,line
R5999	T8702	T8703	compound	tTA,APP
R6000	T8561	T8562	nummod	6,mo
R6001	T8703	T8700	compound	APP,mice
R6002	T8704	T8703	punct	/,APP
R6003	T8562	T8560	pobj	mo,between
R6004	T8705	T8700	acl	treated,mice
R6005	T8706	T8705	prep	with,treated
R6006	T8707	T8706	pobj	dox,with
R6007	T8563	T8561	cc	and,6
R6008	T8564	T8561	conj	9,6
R6009	T8565	T8562	prep	of,mo
R6010	T8566	T8565	pobj	age,of
R6011	T8708	T8705	prep	for,treated
R6012	T8709	T8710	nummod	3,mo
R6013	T8567	T8544	punct	.,worsened
R6014	T8569	T8570	nsubj	Suppression,arrested
R6015	T8571	T8569	prep	of,Suppression
R6016	T8710	T8708	pobj	mo,for
R6017	T8711	T8710	cc	or,mo
R6018	T8712	T8710	nummod	6,mo
R6019	T8572	T8573	compound	transgene,expression
R6020	T8713	T8714	det	the,amount
R6021	T8714	T8689	dobj	amount,had
R6022	T8573	T8571	pobj	expression,of
R6023	T8715	T8714	amod	same,amount
R6024	T8716	T8714	prep	of,amount
R6025	T8574	T8570	advmod	abruptly,arrested
R6026	T8717	T8718	amod	aggregated,Aβ
R6027	T8718	T8716	pobj	Aβ,of
R6028	T8575	T8570	dobj	progression,arrested
R6029	T8719	T8714	prep	as,amount
R6030	T8720	T8721	advmod	when,started
R6031	T8576	T8575	prep	of,progression
R6032	T8721	T8719	pcomp	started,as
R6033	T8722	T8721	nsubj	they,started
R6034	T8723	T8721	dobj	treatment,started
R6035	T8577	T8576	pobj	pathology,of
R6036	T8724	T8721	prep	at,started
R6037	T8725	T8726	nummod	6,mo
R6038	T8578	T8579	punct	(,S6
R6039	T8726	T8724	pobj	mo,at
R6040	T8727	T8726	prep	of,mo
R6041	T8728	T8727	pobj	age,of
R6042	T8729	T8730	punct	(,4A
R6043	T8730	T8689	parataxis	4A,had
R6044	T8579	T8570	parataxis	S6,arrested
R6045	T8731	T8730	compound	Figure,4A
R6046	T8732	T8730	cc	and,4A
R6047	T8733	T8730	conj	4B,4A
R6048	T8580	T8579	compound	Figure,S6
R6049	T8734	T8730	punct	),4A
R6050	T8735	T8689	punct	.,had
R6051	T8581	T8579	punct	),S6
R6052	T8737	T8738	prep	In,had
R6053	T8582	T8570	punct	", ",arrested
R6054	T8739	T8737	pobj	contrast,In
R6055	T8740	T8738	punct	", ",had
R6056	T8583	T8584	cc	but,without
R6057	T8741	T8742	amod	untreated,mice
R6058	T8742	T8738	nsubj	mice,had
R6059	T8584	T8570	prep	without,arrested
R6060	T8743	T8744	nummod	9,mo
R6061	T8744	T8746	npadvmod	mo,old
R6062	T8745	T8744	punct	-,mo
R6063	T8585	T8584	advmod	again,without
R6064	T8746	T8742	amod	old,mice
R6065	T8747	T8746	punct	-,old
R6066	T8586	T8587	det	any,sign
R6067	T8748	T8749	advmod	almost,twice
R6068	T8749	T8750	advmod	twice,much
R6069	T8587	T8584	pobj	sign,without
R6070	T8750	T8752	amod	much,Aβ
R6071	T8751	T8750	advmod	as,much
R6072	T8588	T8587	prep	of,sign
R6073	T8752	T8738	dobj	Aβ,had
R6075	T8754	T8752	prep	as,Aβ
R6076	T8589	T8588	pobj	reduction,of
R6079	T8590	T8570	punct	.,arrested
R6080	T8757	T8758	det	the,groups
R6082	T8759	T8758	amod	treated,groups
R6083	T8592	T8593	preconj	Both,silver
R6084	T8760	T8738	punct	.,had
R6085	T8762	T8763	compound	Filter,analysis
R6086	T8593	T8594	npadvmod	silver,positive
R6087	T8763	T8765	nsubj	analysis,yielded
R6088	T8764	T8763	compound	trap,analysis
R6089	T8765	T8773	ccomp	yielded,arrested
R6090	T8594	T8601	amod	positive,plaques
R6091	T8766	T8763	prep	of,analysis
R6092	T8767	T8768	nmod	line,mice
R6093	T8768	T8766	pobj	mice,of
R6094	T8769	T8767	nummod	18,line
R6095	T8595	T8593	punct	-,silver
R6096	T8770	T8771	compound	tTA,APP
R6097	T8771	T8768	compound	APP,mice
R6098	T8772	T8771	punct	/,APP
R6099	T8596	T8593	cc	and,silver
R6100	T8774	T8775	amod	similar,results
R6101	T8597	T8598	compound	thioflavin,S
R6102	T8775	T8765	dobj	results,yielded
R6103	T8776	T8773	punct	: ,arrested
R6104	T8598	T8593	conj	S,silver
R6105	T8777	T8778	det	the,increase
R6106	T8778	T8773	nsubjpass	increase,arrested
R6107	T8599	T8598	punct	-,S
R6108	T8779	T8778	prep	in,increase
R6109	T8780	T8781	amod	aggregated,Aβ
R6110	T8781	T8779	pobj	Aβ,in
R6111	T8600	T8594	punct	-,positive
R6112	T8782	T8778	acl	observed,increase
R6113	T8783	T8782	prep	in,observed
R6114	T8601	T8602	nsubjpass	plaques,found
R6115	T8784	T8785	amod	untreated,animals
R6116	T8785	T8783	pobj	animals,in
R6117	T8786	T8785	prep	between,animals
R6118	T8814	T8811	prep	in,measure
R6119	T8787	T8788	nummod	6,mo
R6120	T8788	T8786	pobj	mo,between
R6121	T8789	T8787	cc	and,6
R6122	T8790	T8787	conj	9,6
R6123	T8815	T8816	det	the,brains
R6124	T8791	T8788	prep	of,mo
R6125	T8792	T8791	pobj	age,of
R6126	T8793	T8773	auxpass	was,arrested
R6127	T8816	T8814	pobj	brains,in
R6128	T8794	T8773	advmod	completely,arrested
R6129	T8817	T8816	prep	of,brains
R6130	T8818	T8819	det	each,group
R6131	T8795	T8773	agent	by,arrested
R6132	T8796	T8797	compound	transgene,suppression
R6133	T8819	T8817	pobj	group,of
R6134	T8797	T8795	pobj	suppression,by
R6135	T8798	T8799	punct	(,S5C
R6136	T8799	T8773	parataxis	S5C,arrested
R6137	T8820	T8821	aux	to,determine
R6138	T8800	T8799	compound	Figure,S5C
R6139	T8801	T8799	cc	and,S5C
R6140	T8821	T8811	advcl	determine,measure
R6141	T8802	T8799	conj	S5D,S5C
R6142	T8803	T8799	punct	),S5C
R6143	T8822	T8823	mark	whether,occurred
R6144	T8804	T8773	punct	.,arrested
R6145	T8806	T8807	nsubj	We,used
R6146	T8808	T8807	advmod	next,used
R6147	T8823	T8821	ccomp	occurred,determine
R6148	T8809	T8807	dobj	ELISA,used
R6149	T8810	T8811	aux	to,measure
R6150	T8824	T8825	det	any,change
R6151	T8811	T8807	advcl	measure,used
R6152	T8812	T8813	amod	total,Aβ
R6153	T8813	T8811	dobj	Aβ,measure
R6154	T8825	T8823	nsubj	change,occurred
R6155	T8826	T8825	prep	in,change
R6156	T8827	T8828	det	the,amount
R6157	T8828	T8826	pobj	amount,in
R6158	T8829	T8828	cc	or,amount
R6159	T8830	T8828	conj	solubility,amount
R6160	T8920	T8918	prep	with,Consistent
R6161	T8921	T8922	det	the,results
R6162	T8831	T8828	prep	of,amount
R6163	T8922	T8920	pobj	results,with
R6164	T8923	T8922	compound	filter,results
R6165	T8832	T8831	pobj	peptide,of
R6166	T8924	T8922	compound	trap,results
R6167	T8925	T8922	acl	presented,results
R6168	T8926	T8925	advmod	above,presented
R6169	T8833	T8834	mark	while,suppressed
R6170	T8927	T8919	punct	", ",were
R6171	T8928	T8919	expl	there,were
R6172	T8834	T8823	advcl	suppressed,occurred
R6173	T8929	T8930	det	no,differences
R6174	T8930	T8919	attr	differences,were
R6175	T8931	T8930	amod	significant,differences
R6176	T8835	T8836	compound	APPswe,ind
R6177	T8932	T8930	prep	in,differences
R6178	T8933	T8934	npadvmod	SDS,soluble
R6179	T8836	T8838	compound	ind,expression
R6180	T8934	T8939	amod	soluble,Aβ
R6181	T8935	T8933	punct	-,SDS
R6182	T8936	T8933	cc	or,SDS
R6183	T8837	T8836	punct	/,ind
R6184	T8937	T8933	conj	FA,SDS
R6185	T8938	T8934	punct	-,soluble
R6186	T8939	T8932	pobj	Aβ,in
R6187	T8838	T8834	nsubjpass	expression,suppressed
R6188	T8940	T8930	prep	between,differences
R6189	T8941	T8942	det	the,cohort
R6190	T8839	T8834	auxpass	was,suppressed
R6191	T8942	T8940	pobj	cohort,between
R6192	T8943	T8944	nummod	6,mo
R6193	T8944	T8942	nmod	mo,cohort
R6194	T8840	T8807	punct	.,used
R6195	T8945	T8942	amod	untreated,cohort
R6196	T8946	T8942	cc	and,cohort
R6197	T8947	T8948	preconj	either,cohorts
R6198	T8948	T8942	conj	cohorts,cohort
R6199	T8949	T8948	det	the,cohorts
R6200	T8842	T8843	amod	Cortical,homogenates
R6201	T8950	T8951	nummod	6,mo
R6202	T8951	T8948	nmod	mo,cohorts
R6203	T8952	T8951	punct	+,mo
R6204	T8843	T8844	nsubjpass	homogenates,extracted
R6205	T8953	T8954	nummod	3,mo
R6206	T8954	T8951	appos	mo,mo
R6207	T8955	T8954	acl	treated,mo
R6208	T8844	T8847	ccomp	extracted,measured
R6209	T8956	T8951	cc	or,mo
R6210	T8957	T8958	nummod	6,mo
R6211	T8958	T8951	conj	mo,mo
R6212	T8845	T8844	auxpass	were,extracted
R6213	T8959	T8958	punct	+,mo
R6214	T8960	T8961	nummod	6,mo
R6215	T8846	T8844	advmod	sequentially,extracted
R6216	T8961	T8958	appos	mo,mo
R6217	T8962	T8961	acl	treated,mo
R6218	T8963	T8919	punct	.,were
R6219	T8848	T8849	aux	to,separate
R6220	T8965	T8966	advmod	However,contained
R6221	T8849	T8844	advcl	separate,extracted
R6222	T8850	T8849	dobj	peptide,separate
R6223	T8967	T8966	punct	", ",contained
R6224	T8968	T8966	nsubj	brains,contained
R6225	T8851	T8849	prep	into,separate
R6226	T8969	T8968	prep	of,brains
R6227	T8970	T8971	preconj	both,mo
R6228	T8971	T8973	npadvmod	mo,treated
R6229	T8852	T8853	npadvmod	PBS,soluble
R6230	T8972	T8971	nummod	6,mo
R6231	T8973	T8983	amod	treated,cohorts
R6232	T8853	T8862	amod	soluble,fractions
R6233	T8974	T8971	punct	+,mo
R6234	T8975	T8976	nummod	3,mo
R6235	T8854	T8852	punct	-,PBS
R6236	T8976	T8971	appos	mo,mo
R6237	T8977	T8971	cc	and,mo
R6238	T8978	T8979	nummod	6,mo
R6239	T8855	T8852	punct	", ",PBS
R6240	T8979	T8971	conj	mo,mo
R6241	T8980	T8979	punct	+,mo
R6242	T8981	T8982	nummod	6,mo
R6243	T8856	T8852	conj	SDS,PBS
R6244	T8982	T8979	appos	mo,mo
R6245	T8983	T8969	pobj	cohorts,of
R6246	T8857	T8856	punct	-,SDS
R6247	T8984	T8985	advmod	roughly,twice
R6248	T8985	T8986	advmod	twice,much
R6249	T8986	T8988	amod	much,Aβ40
R6250	T8987	T8986	advmod	as,much
R6251	T8988	T8966	dobj	Aβ40,contained
R6252	T8858	T8856	punct	", ",SDS
R6253	T8989	T8988	nmod	PBS,Aβ40
R6254	T8990	T8988	punct	-,Aβ40
R6255	T8991	T8988	amod	soluble,Aβ40
R6256	T8859	T8856	cc	and,SDS
R6257	T8992	T8988	prep	as,Aβ40
R6258	T8993	T8994	amod	untreated,mice
R6259	T8860	T8856	conj	FA,SDS
R6260	T8994	T8992	pobj	mice,as
R6261	T8995	T8996	nummod	6,mo
R6262	T8996	T8998	npadvmod	mo,old
R6263	T8861	T8853	punct	-,soluble
R6264	T8997	T8996	punct	-,mo
R6265	T8998	T8994	amod	old,mice
R6266	T8862	T8851	pobj	fractions,into
R6267	T8999	T8998	punct	-,old
R6268	T9000	T9001	punct	(,5C
R6269	T8863	T8847	punct	", ",measured
R6270	T9001	T8966	parataxis	5C,contained
R6271	T9002	T9001	compound	Figure,5C
R6272	T9003	T9001	punct	),5C
R6273	T8864	T8847	advmod	then,measured
R6274	T9004	T8966	punct	.,contained
R6275	T8865	T8866	npadvmod	transgene,derived
R6276	T9006	T9007	nsubj	Levels,showed
R6277	T9008	T9006	prep	of,Levels
R6278	T8866	T8868	amod	derived,Aβ40
R6279	T9009	T9008	pobj	Aβ42,of
R6280	T9010	T9011	det	a,trend
R6281	T8867	T8866	punct	-,derived
R6282	T9011	T9007	dobj	trend,showed
R6283	T9012	T9011	amod	similar,trend
R6284	T9013	T9007	punct	", ",showed
R6285	T8868	T8847	nsubjpass	Aβ40,measured
R6286	T9014	T9007	cc	but,showed
R6287	T9015	T9016	aux	did,reach
R6288	T8869	T8868	cc	and,Aβ40
R6289	T9016	T9007	conj	reach,showed
R6290	T9017	T9016	neg	not,reach
R6291	T9018	T9019	amod	statistical,significance
R6292	T8870	T8868	conj	Aβ42,Aβ40
R6293	T9019	T9016	dobj	significance,reach
R6294	T9020	T9007	punct	.,showed
R6295	T8871	T8847	auxpass	were,measured
R6296	T9022	T9023	prep	In,were
R6297	T8872	T8847	agent	by,measured
R6298	T9024	T9022	pobj	fact,In
R6299	T8873	T8874	amod	human,specific
R6300	T9025	T9023	punct	", ",were
R6301	T9026	T9023	nsubj	levels,were
R6302	T8874	T8876	amod	specific,ELISA
R6303	T9027	T9026	prep	of,levels
R6304	T9028	T9029	npadvmod	PBS,soluble
R6305	T9029	T9031	amod	soluble,Aβ40
R6306	T8875	T8874	punct	-,specific
R6307	T9030	T9029	punct	-,soluble
R6308	T9031	T9027	pobj	Aβ40,of
R6309	T8876	T8872	pobj	ELISA,by
R6310	T9032	T9031	cc	and,Aβ40
R6311	T9033	T9031	conj	Aβ42,Aβ40
R6312	T9034	T9026	prep	in,levels
R6313	T8877	T8878	punct	[,23
R6314	T9035	T9036	det	the,cohorts
R6315	T9036	T9034	pobj	cohorts,in
R6316	T8878	T8847	parataxis	23,measured
R6317	T9037	T9038	nummod	6,mo
R6318	T9038	T9036	nmod	mo,cohorts
R6319	T9039	T9038	punct	+,mo
R6320	T8879	T8878	punct	],23
R6321	T9040	T9041	nummod	3,mo
R6322	T9041	T9038	appos	mo,mo
R6323	T8880	T8847	punct	.,measured
R6324	T9042	T9038	cc	and,mo
R6325	T9043	T9044	nummod	6,mo
R6326	T9044	T9038	conj	mo,mo
R6327	T8882	T8883	prep	In,found
R6328	T9045	T9044	punct	+,mo
R6329	T9046	T9047	nummod	6,mo
R6330	T9047	T9044	appos	mo,mo
R6331	T9048	T9044	acl	treated,mo
R6332	T8884	T8885	det	all,animals
R6333	T9049	T9050	advmod	most,similar
R6334	T9050	T9023	acomp	similar,were
R6335	T9051	T9050	prep	to,similar
R6336	T8885	T8882	pobj	animals,In
R6337	T8886	T8885	acl	harboring,animals
R6338	T9052	T9051	pobj	that,to
R6339	T9053	T9052	prep	of,that
R6340	T8887	T8888	compound	amyloid,deposits
R6341	T9054	T9055	det	the,cohort
R6342	T9055	T9053	pobj	cohort,of
R6343	T9056	T9057	nummod	9,mo
R6344	T8888	T8886	dobj	deposits,harboring
R6345	T9057	T9055	nmod	mo,cohort
R6346	T9058	T9055	amod	untreated,cohort
R6347	T8889	T8883	punct	", ",found
R6348	T9059	T9023	punct	", ",were
R6349	T9060	T9023	advcl	suggesting,were
R6350	T8890	T8883	nsubj	we,found
R6351	T9061	T9062	mark	that,determine
R6352	T9062	T9060	ccomp	determine,suggesting
R6353	T9063	T9062	nsubj	age,determine
R6354	T9064	T9063	punct	", ",age
R6355	T9065	T9063	prep	as,age
R6356	T9066	T9065	prep	opposed,as
R6357	T8891	T8892	mark	that,extracted
R6358	T9067	T9066	prep	to,opposed
R6359	T9068	T9069	amod	synthetic,rate
R6360	T8892	T8883	ccomp	extracted,found
R6361	T9069	T9067	pobj	rate,to
R6362	T9070	T9069	punct	(,rate
R6363	T9071	T9072	dep	which,be
R6364	T8893	T8894	det	the,majority
R6365	T9072	T9069	relcl	be,rate
R6366	T9073	T9072	aux	would,be
R6367	T8894	T8892	nsubjpass	majority,extracted
R6368	T9074	T9072	acomp	negligible,be
R6369	T9075	T9072	prep	in,be
R6370	T9076	T9077	det	the,animals
R6371	T8895	T8894	amod	vast,majority
R6372	T9077	T9075	pobj	animals,in
R6373	T9078	T9077	amod	treated,animals
R6374	T9079	T9062	punct	),determine
R6375	T8896	T8894	prep	of,majority
R6376	T9080	T9062	punct	", ",determine
R6377	T9081	T9062	aux	may,determine
R6378	T9082	T9083	det	the,fraction
R6379	T8897	T8896	pobj	Aβ,of
R6380	T9083	T9062	dobj	fraction,determine
R6381	T9084	T9083	prep	of,fraction
R6382	T8898	T8899	punct	(,%
R6383	T9085	T9086	npadvmod	PBS,soluble
R6384	T9086	T9088	amod	soluble,Aβ
R6385	T9087	T9086	punct	-,soluble
R6386	T8899	T8897	parataxis	%,Aβ
R6387	T9088	T9084	pobj	Aβ,of
R6388	T9089	T9062	prep	in,determine
R6389	T9090	T9091	det	these,animals
R6390	T8900	T8901	punct	>,99
R6391	T9091	T9089	pobj	animals,in
R6392	T9092	T9023	punct	.,were
R6393	T8901	T8899	nummod	99,%
R6394	T9094	T9095	nsubj	We,assessed
R6395	T8902	T8899	punct	),%
R6396	T9096	T9095	advmod	also,assessed
R6397	T9097	T9098	amod	neuritic,pathology
R6398	T8903	T8892	auxpass	was,extracted
R6399	T9098	T9095	dobj	pathology,assessed
R6400	T9099	T9097	cc	and,neuritic
R6401	T9100	T9097	conj	glial,neuritic
R6402	T8904	T8892	prep	into,extracted
R6403	T9101	T9098	acl	surrounding,pathology
R6404	T9102	T9103	det	the,plaques
R6405	T9103	T9101	dobj	plaques,surrounding
R6406	T9104	T9105	aux	to,determine
R6407	T9105	T9095	advcl	determine,assessed
R6408	T8905	T8906	det	the,fractions
R6409	T9106	T9107	mark	whether,were
R6410	T9107	T9105	ccomp	were,determine
R6411	T8906	T8904	pobj	fractions,into
R6412	T9108	T9107	expl	there,were
R6415	T8907	T8906	nmod	SDS,fractions
R6416	T9111	T9110	prep	in,changes
R6417	T9112	T9113	amod	nearby,tissue
R6418	T8908	T8907	cc	and,SDS
R6419	T9113	T9111	pobj	tissue,in
R6420	T9114	T9107	prep	following,were
R6421	T9115	T9116	amod	long,term
R6422	T8909	T8907	conj	FA,SDS
R6423	T9116	T9118	compound	term,suppression
R6424	T9117	T9116	punct	-,term
R6425	T8910	T8911	punct	(,5A
R6426	T9118	T9114	pobj	suppression,following
R6427	T9119	T9118	compound	transgene,suppression
R6428	T9120	T9095	punct	.,assessed
R6429	T8911	T8892	parataxis	5A,extracted
R6430	T9122	T9123	preconj	Both,stain
R6431	T8912	T8911	compound	Figure,5A
R6432	T9123	T9126	nsubj	stain,showed
R6433	T9124	T9123	compound	Hirano,stain
R6434	T9125	T9123	compound	silver,stain
R6435	T8913	T8911	cc	and,5A
R6436	T9127	T9123	cc	and,stain
R6437	T9128	T9129	compound	ubiquitin,immunostaining
R6438	T8914	T8911	conj	5B,5A
R6439	T9129	T9123	conj	immunostaining,stain
R6440	T9130	T9131	amod	neuritic,pathology
R6441	T8915	T8911	punct	),5A
R6442	T8916	T8883	punct	.,found
R6443	T8918	T8919	advcl	Consistent,were
R6444	T9131	T9126	dobj	pathology,showed
R6445	T9132	T9126	prep	in,showed
R6446	T9237	T9234	auxpass	was,interrupted
R6447	T9133	T9134	det	all,groups
R6448	T9134	T9132	pobj	groups,in
R6449	T9135	T9134	compound	treatment,groups
R6450	T9238	T9210	punct	.,was
R6451	T9136	T9137	punct	(,Figure
R6452	T9240	T9241	advmod	Unfortunately,compromised
R6453	T9137	T9126	parataxis	Figure,showed
R6454	T9138	T9137	nummod	6,Figure
R6455	T9139	T9137	punct	),Figure
R6456	T9140	T9126	punct	.,showed
R6457	T9242	T9241	punct	", ",compromised
R6458	T9142	T9143	advmod	Similarly,found
R6459	T9144	T9143	punct	", ",found
R6460	T9145	T9146	amod	activated,astrocytes
R6461	T9243	T9241	nsubjpass	efforts,compromised
R6462	T9146	T9143	nsubjpass	astrocytes,found
R6463	T9147	T9146	acl	immunostained,astrocytes
R6464	T9244	T9245	aux	to,characterize
R6465	T9148	T9147	prep	for,immunostained
R6466	T9149	T9148	pobj	GFAP,for
R6467	T9245	T9243	acl	characterize,efforts
R6468	T9150	T9143	auxpass	were,found
R6469	T9151	T9143	prep	near,found
R6470	T9152	T9151	pobj	plaques,near
R6471	T9246	T9247	amod	cognitive,behavior
R6472	T9153	T9143	prep	in,found
R6473	T9154	T9155	det	all,animals
R6474	T9247	T9245	dobj	behavior,characterize
R6475	T9155	T9153	pobj	animals,in
R6476	T9156	T9157	punct	(,Figure
R6477	T9248	T9241	auxpass	were,compromised
R6478	T9157	T9143	parataxis	Figure,found
R6479	T9158	T9157	nummod	6,Figure
R6480	T9159	T9157	punct	),Figure
R6481	T9249	T9241	agent	by,compromised
R6482	T9160	T9143	punct	.,found
R6483	T9162	T9163	amod	Neuritic,pathology
R6484	T9250	T9251	amod	severe,hyperactivity
R6485	T9163	T9166	nsubj	pathology,were
R6486	T9164	T9162	cc	and,Neuritic
R6487	T9251	T9249	pobj	hyperactivity,by
R6488	T9165	T9162	conj	glial,Neuritic
R6489	T9167	T9168	advmod	more,severe
R6490	T9252	T9251	prep	in,hyperactivity
R6491	T9168	T9166	acomp	severe,were
R6492	T9169	T9166	prep	in,were
R6493	T9253	T9254	amod	untreated,mice
R6494	T9170	T9171	det	the,mice
R6495	T9171	T9169	pobj	mice,in
R6496	T9172	T9171	amod	older,mice
R6497	T9254	T9252	pobj	mice,in
R6498	T9173	T9171	amod	untreated,mice
R6499	T9174	T9166	punct	.,were
R6500	T9255	T9256	amod	double,transgenic
R6501	T9176	T9177	prep	In,prevented
R6502	T9256	T9254	amod	transgenic,mice
R6503	T9178	T9176	pobj	contrast,In
R6504	T9179	T9177	punct	", ",prevented
R6505	T9180	T9181	compound	transgene,suppression
R6506	T9257	T9241	punct	.,compromised
R6507	T9181	T9177	nsubj	suppression,prevented
R6508	T9182	T9183	det	the,growth
R6509	T9183	T9177	dobj	growth,prevented
R6510	T9184	T9183	prep	of,growth
R6511	T9185	T9186	amod	individual,deposits
R6512	T9259	T9260	det	The,animals
R6513	T9186	T9184	pobj	deposits,of
R6514	T9187	T9186	relcl	apparent,deposits
R6515	T9188	T9187	prep	in,apparent
R6516	T9189	T9190	amod	untreated,mice
R6517	T9260	T9264	nsubjpass	animals,seen
R6518	T9190	T9188	pobj	mice,in
R6519	T9191	T9177	punct	", ",prevented
R6520	T9192	T9177	cc	and,prevented
R6521	T9261	T9262	compound	tTA,APP
R6522	T9193	T9177	conj	limited,prevented
R6523	T9194	T9195	det	the,pathology
R6524	T9262	T9260	compound	APP,animals
R6525	T9195	T9193	dobj	pathology,limited
R6526	T9196	T9195	amod	surrounding,pathology
R6527	T9197	T9193	prep	to,limited
R6528	T9263	T9262	punct	/,APP
R6529	T9198	T9199	dep	what,was
R6530	T9199	T9197	pcomp	was,to
R6531	T9265	T9264	auxpass	were,seen
R6532	T9200	T9199	advmod	already,was
R6533	T9201	T9199	acomp	present,was
R6534	T9202	T9203	advmod	when,began
R6535	T9203	T9199	advcl	began,was
R6536	T9266	T9264	advmod	often,seen
R6537	T9204	T9203	nsubj	treatment,began
R6538	T9267	T9264	advcl	running,seen
R6539	T9268	T9267	prep	in,running
R6540	T9205	T9177	punct	.,prevented
R6541	T9269	T9268	pobj	circles,in
R6542	T9207	T9208	det	An,question
R6543	T9270	T9267	prep	around,running
R6544	T9208	T9210	nsubj	question,was
R6545	T9209	T9208	amod	obvious,question
R6546	T9271	T9272	det	the,perimeter
R6547	T9211	T9212	nsubj	we,sought
R6548	T9212	T9208	advcl	sought,question
R6549	T9272	T9270	pobj	perimeter,around
R6550	T9213	T9214	aux	to,address
R6551	T9214	T9212	xcomp	address,sought
R6552	T9215	T9216	mark	whether,diminished
R6553	T9273	T9272	prep	of,perimeter
R6554	T9216	T9210	attr	diminished,was
R6555	T9217	T9218	det	the,deposition
R6556	T9218	T9216	nsubj	deposition,diminished
R6557	T9274	T9275	poss	their,cages
R6558	T9219	T9218	prep	of,deposition
R6559	T9220	T9219	pobj	Aβ,of
R6560	T9221	T9222	amod	cognitive,ability
R6561	T9222	T9216	dobj	ability,diminished
R6562	T9223	T9222	prep	in,ability
R6563	T9275	T9273	pobj	cages,of
R6564	T9224	T9225	amod	untreated,mice
R6565	T9225	T9223	pobj	mice,in
R6568	T9226	T9216	punct	", ",diminished
R6569	T9227	T9216	cc	and,diminished
R6570	T9278	T9279	det	a,pattern
R6571	T9228	T9229	dep	what,happen
R6572	T9229	T9216	conj	happen,diminished
R6573	T9279	T9282	nsubjpass	pattern,noted
R6574	T9230	T9229	aux	might,happen
R6575	T9231	T9229	prep	to,happen
R6576	T9232	T9231	pobj	cognition,to
R6577	T9280	T9279	amod	similar,pattern
R6578	T9233	T9234	advmod	when,interrupted
R6579	T9281	T9279	compound	swimming,pattern
R6580	T9234	T9229	advcl	interrupted,happen
R6581	T9235	T9236	det	the,process
R6582	T9236	T9234	nsubjpass	process,interrupted
R6583	T9282	T9264	conj	noted,seen
R6584	T9283	T9282	auxpass	was,noted
R6585	T9284	T9285	advmod	when,tested
R6586	T9285	T9282	advcl	tested,noted
R6587	T9343	T9344	amod	certain,criteria
R6588	T9286	T9287	det	the,mice
R6589	T9344	T9342	dobj	criteria,meet
R6590	T9345	T9344	compound	performance,criteria
R6591	T9346	T9347	punct	[,30
R6592	T9287	T9285	nsubjpass	mice,tested
R6593	T9347	T9319	parataxis	30,dealt
R6594	T9348	T9347	punct	],30
R6595	T9288	T9285	auxpass	were,tested
R6596	T9349	T9319	punct	.,dealt
R6597	T9289	T9285	prep	in,tested
R6598	T9351	T9352	prep	In,was
R6599	T9290	T9291	det	the,maze
R6600	T9353	T9354	poss	our,case
R6601	T9354	T9351	pobj	case,In
R6602	T9291	T9289	pobj	maze,in
R6603	T9355	T9352	punct	", ",was
R6604	T9356	T9357	det	the,penetrance
R6605	T9357	T9352	nsubj	penetrance,was
R6606	T9292	T9291	compound	Morris,maze
R6607	T9358	T9357	prep	of,penetrance
R6608	T9359	T9358	pobj	hyperactivity,of
R6609	T9293	T9291	compound	water,maze
R6610	T9360	T9361	amod	close,100
R6611	T9361	T9363	nummod	100,%
R6612	T9362	T9361	quantmod	to,100
R6613	T9363	T9352	attr	%,was
R6614	T9364	T9352	punct	", ",was
R6615	T9294	T9282	punct	.,noted
R6616	T9365	T9352	advcl	leaving,was
R6617	T9366	T9365	dobj	us,leaving
R6618	T9367	T9365	prep	with,leaving
R6619	T9296	T9297	prep	In,noted
R6620	T9368	T9369	det	no,animals
R6621	T9369	T9367	pobj	animals,with
R6622	T9370	T9369	amod	testable,animals
R6623	T9298	T9299	det	the,maze
R6624	T9371	T9352	punct	.,was
R6625	T9373	T9374	det	This,phenotype
R6626	T9374	T9375	nsubj	phenotype,affected
R6627	T9299	T9296	pobj	maze,In
R6628	T9300	T9299	amod	radial,maze
R6629	T9376	T9375	aux	has,affected
R6630	T9377	T9375	neg	not,affected
R6631	T9378	T9379	amod	previous,lines
R6632	T9301	T9299	compound	water,maze
R6633	T9379	T9375	dobj	lines,affected
R6634	T9380	T9379	prep	of,lines
R6635	T9302	T9297	punct	", ",noted
R6636	T9381	T9382	compound	APPswe,mice
R6637	T9382	T9380	pobj	mice,of
R6638	T9303	T9304	amod	repetitive,patterns
R6639	T9383	T9384	nsubj	we,produced
R6640	T9384	T9379	advcl	produced,lines
R6641	T9304	T9297	nsubjpass	patterns,noted
R6642	T9385	T9384	aux	have,produced
R6643	T9386	T9379	punct	", ",lines
R6644	T9387	T9388	amod	such,as
R6645	T9305	T9304	compound	swim,patterns
R6646	T9388	T9379	prep	as,lines
R6647	T9389	T9390	compound	lines,E1
R6648	T9306	T9297	auxpass	were,noted
R6649	T9390	T9388	pobj	E1,as
R6650	T9391	T9390	punct	–,E1
R6651	T9392	T9390	nummod	2,E1
R6652	T9307	T9297	prep	with,noted
R6653	T9393	T9390	cc	or,E1
R6654	T9394	T9390	conj	C3,E1
R6655	T9308	T9309	det	no,evidence
R6656	T9395	T9394	punct	–,C3
R6657	T9396	T9394	nummod	3,C3
R6658	T9309	T9307	pobj	evidence,with
R6659	T9397	T9379	punct	", ",lines
R6660	T9398	T9399	dep	that,express
R6661	T9399	T9379	relcl	express,lines
R6662	T9310	T9309	prep	of,evidence
R6663	T9311	T9312	npadvmod	choice,motivated
R6664	T9400	T9401	amod	lower,levels
R6665	T9401	T9399	dobj	levels,express
R6666	T9402	T9401	prep	of,levels
R6667	T9312	T9314	amod	motivated,actions
R6668	T9403	T9404	amod	transgenic,protein
R6669	T9404	T9402	pobj	protein,of
R6670	T9313	T9312	punct	-,motivated
R6671	T9405	T9375	punct	.,affected
R6672	T9314	T9310	pobj	actions,of
R6673	T9407	T9408	advmod	Indeed,established
R6674	T9409	T9408	punct	", ",established
R6675	T9315	T9297	punct	.,noted
R6676	T9410	T9408	prep	in,established
R6677	T9411	T9412	amod	past,studies
R6678	T9317	T9318	amod	Other,studies
R6679	T9412	T9410	pobj	studies,in
R6680	T9413	T9414	advmod	where,was
R6681	T9414	T9412	relcl	was,studies
R6682	T9318	T9319	nsubj	studies,dealt
R6683	T9415	T9414	nsubj	hyperactivity,was
R6684	T9416	T9414	neg	not,was
R6685	T9417	T9418	det	a,factor
R6686	T9320	T9319	aux	have,dealt
R6687	T9418	T9414	attr	factor,was
R6688	T9419	T9408	punct	", ",established
R6689	T9420	T9408	nsubj	we,established
R6690	T9421	T9422	det	a,relationship
R6691	T9321	T9319	prep	with,dealt
R6692	T9422	T9408	dobj	relationship,established
R6693	T9423	T9422	amod	clear,relationship
R6694	T9322	T9323	amod	similar,problems
R6695	T9424	T9422	prep	between,relationship
R6696	T9425	T9426	compound	amyloid,load
R6697	T9426	T9424	pobj	load,between
R6698	T9323	T9321	pobj	problems,with
R6699	T9427	T9426	cc	and,load
R6700	T9428	T9429	amod	cognitive,ability
R6701	T9324	T9319	prep	by,dealt
R6702	T9429	T9426	conj	ability,load
R6703	T9430	T9431	punct	[,31
R6704	T9431	T9408	parataxis	31,established
R6705	T9325	T9324	pcomp	excluding,by
R6706	T9432	T9431	punct	],31
R6707	T9433	T9408	punct	.,established
R6708	T9326	T9325	dobj	animals,excluding
R6709	T9435	T9436	advmod	However,feel
R6710	T9327	T9328	dep	that,show
R6711	T9437	T9436	punct	", ",feel
R6712	T9438	T9436	prep	in,feel
R6713	T9439	T9440	det	the,study
R6714	T9328	T9326	relcl	show,animals
R6715	T9440	T9438	pobj	study,in
R6716	T9441	T9440	amod	current,study
R6717	T9442	T9436	punct	", ",feel
R6718	T9443	T9436	nsubj	we,feel
R6719	T9444	T9445	mark	that,made
R6720	T9329	T9328	aux	do,show
R6721	T9445	T9436	ccomp	made,feel
R6722	T9446	T9447	mark	although,scored
R6723	T9447	T9445	advcl	scored,made
R6724	T9330	T9328	neg	not,show
R6725	T9448	T9449	det	the,performance
R6726	T9331	T9332	amod	adequate,attention
R6727	T9332	T9328	dobj	attention,show
R6728	T9449	T9447	nsubjpass	performance,scored
R6729	T9333	T9332	prep	to,attention
R6730	T9450	T9449	amod	poor,performance
R6731	T9451	T9449	prep	of,performance
R6732	T9334	T9335	det	the,task
R6733	T9452	T9453	det	the,mice
R6734	T9453	T9451	pobj	mice,of
R6735	T9454	T9455	compound	tTA,APP
R6736	T9335	T9333	pobj	task,to
R6737	T9455	T9453	compound	APP,mice
R6738	T9456	T9455	punct	/,APP
R6739	T9336	T9328	punct	", ",show
R6740	T9457	T9449	prep	in,performance
R6741	T9458	T9459	det	the,tests
R6742	T9459	T9457	pobj	tests,in
R6743	T9337	T9328	advcl	retaining,show
R6744	T9460	T9459	compound	maze,tests
R6745	T9461	T9447	aux	could,scored
R6746	T9462	T9447	advmod	technically,scored
R6747	T9338	T9339	advmod	only,mice
R6748	T9463	T9447	auxpass	be,scored
R6749	T9464	T9447	prep	as,scored
R6750	T9339	T9337	dobj	mice,retaining
R6751	T9465	T9466	amod	cognitive,impairment
R6752	T9466	T9464	pobj	impairment,as
R6753	T9467	T9445	punct	", ",made
R6754	T9340	T9339	det	those,mice
R6755	T9468	T9469	det	the,animals
R6756	T9469	T9470	poss	animals,hyperactivity
R6757	T9341	T9342	dep	that,meet
R6758	T9470	T9445	nsubj	hyperactivity,made
R6759	T9471	T9469	case	',animals
R6760	T9472	T9470	amod	severe,hyperactivity
R6761	T9342	T9339	relcl	meet,mice
R6762	T9473	T9474	nsubj	interpretation,impossible
R6763	T9474	T9445	ccomp	impossible,made
R6764	T9475	T9473	prep	of,interpretation
R6765	T9555	T9554	punct	–,night
R6766	T9476	T9477	det	the,tasks
R6767	T9477	T9475	pobj	tasks,of
R6768	T9478	T9477	amod	cognitive,tasks
R6769	T9479	T9436	punct	.,feel
R6770	T9481	T9482	prep	In,quantified
R6771	T9556	T9532	prep	than,were
R6772	T9483	T9481	pobj	order,In
R6773	T9484	T9485	aux	to,understand
R6774	T9557	T9556	pobj	any,than
R6775	T9485	T9483	acl	understand,order
R6776	T9558	T9557	prep	of,any
R6777	T9559	T9560	det	the,groups
R6778	T9486	T9487	det	the,nature
R6779	T9487	T9485	dobj	nature,understand
R6780	T9560	T9558	pobj	groups,of
R6781	T9488	T9487	cc	and,nature
R6782	T9489	T9487	conj	extent,nature
R6783	T9490	T9487	prep	of,nature
R6784	T9561	T9560	compound	control,groups
R6785	T9491	T9490	pobj	hyperactivity,of
R6786	T9492	T9491	prep	in,hyperactivity
R6787	T9562	T9532	punct	.,were
R6788	T9493	T9494	det	the,mice
R6789	T9494	T9492	pobj	mice,in
R6790	T9564	T9565	compound	Activity,levels
R6791	T9495	T9496	compound	tTA,APP
R6792	T9496	T9494	compound	APP,mice
R6793	T9497	T9496	punct	/,APP
R6794	T9498	T9482	punct	", ",quantified
R6795	T9565	T9566	nsubj	levels,appeared
R6796	T9499	T9482	nsubj	we,quantified
R6797	T9500	T9501	amod	daily,levels
R6798	T9567	T9568	aux	to,follow
R6799	T9501	T9482	dobj	levels,quantified
R6800	T9502	T9501	compound	activity,levels
R6801	T9503	T9501	prep	in,levels
R6802	T9504	T9505	amod	double,transgenic
R6803	T9568	T9566	xcomp	follow,appeared
R6804	T9505	T9506	amod	transgenic,animals
R6805	T9506	T9503	pobj	animals,in
R6806	T9569	T9570	det	a,cycle
R6807	T9507	T9501	prep	along,levels
R6808	T9508	T9507	prep	with,along
R6809	T9509	T9510	poss	their,siblings
R6810	T9570	T9568	dobj	cycle,follow
R6811	T9510	T9508	pobj	siblings,with
R6812	T9511	T9512	amod	single,transgenic
R6813	T9571	T9572	advmod	relatively,normal
R6814	T9512	T9510	amod	transgenic,siblings
R6815	T9513	T9512	cc	and,transgenic
R6816	T9514	T9512	conj	nontransgenic,transgenic
R6817	T9572	T9570	amod	normal,cycle
R6818	T9515	T9510	acl	using,siblings
R6819	T9516	T9517	nummod	four,beam
R6820	T9517	T9519	compound	beam,frames
R6821	T9518	T9517	punct	-,beam
R6822	T9519	T9515	dobj	frames,using
R6823	T9573	T9570	amod	diurnal,cycle
R6824	T9520	T9519	acl	designed,frames
R6825	T9521	T9522	aux	to,monitor
R6826	T9522	T9520	advcl	monitor,designed
R6827	T9574	T9568	punct	", ",follow
R6828	T9523	T9522	dobj	ambulation,monitor
R6829	T9524	T9522	prep	within,monitor
R6830	T9575	T9568	advcl	decreasing,follow
R6831	T9525	T9526	det	an,cage
R6832	T9526	T9524	pobj	cage,within
R6833	T9527	T9526	amod	enclosed,cage
R6834	T9576	T9575	advmod	substantially,decreasing
R6835	T9528	T9482	punct	.,quantified
R6836	T9577	T9575	prep	during,decreasing
R6837	T9530	T9531	mark	As,shown
R6838	T9531	T9532	advcl	shown,were
R6839	T9578	T9579	det	the,hours
R6840	T9533	T9531	prep	in,shown
R6841	T9534	T9533	pobj	Figure,in
R6842	T9535	T9534	nummod	7,Figure
R6843	T9579	T9577	pobj	hours,during
R6844	T9536	T9532	punct	", ",were
R6845	T9537	T9538	det	the,mice
R6846	T9580	T9579	compound	daylight,hours
R6847	T9538	T9532	nsubj	mice,were
R6848	T9539	T9540	amod	double,transgenic
R6849	T9540	T9538	nmod	transgenic,mice
R6850	T9581	T9566	punct	.,appeared
R6851	T9541	T9542	quantmod	up,10-fold
R6852	T9542	T9544	advmod	10-fold,active
R6853	T9543	T9542	quantmod	to,10-fold
R6855	T9544	T9532	acomp	active,were
R6856	T9545	T9544	advmod	more,active
R6857	T9546	T9532	prep	during,were
R6858	T9547	T9548	det	the,phase
R6859	T9585	T9584	punct	", ",remained
R6860	T9548	T9546	pobj	phase,during
R6861	T9549	T9548	compound	dark,phase
R6862	T9550	T9548	prep	of,phase
R6863	T9586	T9587	advmod	even,during
R6864	T9551	T9552	det	the,cycle
R6865	T9552	T9550	pobj	cycle,of
R6866	T9553	T9554	compound	day,night
R6867	T9587	T9584	prep	during,remained
R6868	T9554	T9552	compound	night,cycle
R6869	T9588	T9589	det	the,phase
R6870	T9589	T9587	pobj	phase,during
R6871	T9590	T9589	compound	light,phase
R6872	T9591	T9584	punct	", ",remained
R6874	T9592	T9593	det	the,mice
R6877	T9593	T9584	nsubj	mice,remained
R6880	T9594	T9595	compound	tTA,APP
R6883	T9595	T9593	compound	APP,mice
R6886	T9596	T9595	punct	/,APP
R6889	T9597	T9598	advmod	many-fold,more
R6892	T9598	T9599	advmod	more,active
R6895	T9599	T9584	acomp	active,remained
R6899	T9600	T9599	prep	than,active
R6903	T9601	T9602	amod	normal,controls
R6906	T9602	T9600	pobj	controls,than
R6910	T9603	T9584	punct	.,remained
R6913	T9605	T9606	det	This,behavior
R6918	T9606	T9607	nsubjpass	behavior,reversed
R6921	T9608	T9607	auxpass	was,reversed
R6922	T9697	T9694	pobj	distinction,with
R6923	T9698	T9697	prep	between,distinction
R6924	T9699	T9700	poss	their,levels
R6925	T9700	T9698	pobj	levels,between
R6926	T9701	T9700	nmod	day,levels
R6927	T9702	T9701	punct	-,day
R6928	T9703	T9701	cc	and,day
R6929	T9609	T9607	advmod	partially,reversed
R6930	T9704	T9701	conj	nighttime,day
R6931	T9705	T9700	compound	activity,levels
R6932	T9706	T9674	punct	.,showed
R6933	T9610	T9609	punct	", ",partially
R6934	T9611	T9609	cc	but,partially
R6935	T9612	T9611	neg	not,but
R6936	T9613	T9609	conj	consistently,partially
R6937	T9614	T9607	punct	", ",reversed
R6938	T9615	T9607	prep	by,reversed
R6939	T9616	T9617	nummod	1,mo
R6940	T9617	T9615	pobj	mo,by
R6941	T9618	T9617	prep	of,mo
R6942	T9619	T9620	compound	transgene,suppression
R6943	T9620	T9618	pobj	suppression,of
R6944	T9621	T9617	acl	beginning,mo
R6945	T9622	T9621	prep	at,beginning
R6946	T9623	T9624	quantmod	4,5
R6947	T9624	T9626	nummod	5,mo
R6948	T9625	T9624	punct	–,5
R6949	T9626	T9622	pobj	mo,at
R6950	T9627	T9626	prep	of,mo
R6951	T9628	T9627	pobj	age,of
R6952	T9629	T9630	punct	(,shown
R6953	T9630	T9607	parataxis	shown,reversed
R6954	T9631	T9630	nsubj	data,shown
R6955	T9632	T9630	neg	not,shown
R6956	T9633	T9630	punct	),shown
R6957	T9634	T9607	punct	.,reversed
R6958	T9636	T9637	prep	In,abolished
R6959	T9638	T9636	pobj	contrast,In
R6960	T9639	T9637	punct	", ",abolished
R6961	T9640	T9637	nsubjpass	hyperactivity,abolished
R6962	T9641	T9637	auxpass	was,abolished
R6963	T9642	T9637	advmod	completely,abolished
R6964	T9643	T9637	prep	by,abolished
R6965	T9644	T9643	pcomp	rearing,by
R6966	T9645	T9646	compound	tTA,APP
R6967	T9646	T9648	compound	APP,mice
R6968	T9647	T9646	punct	/,APP
R6969	T9648	T9644	dobj	mice,rearing
R6970	T9649	T9644	prep	on,rearing
R6971	T9650	T9649	pobj	dox,on
R6972	T9651	T9637	punct	.,abolished
R6973	T9653	T9654	nsubj	Animals,showed
R6974	T9655	T9653	acl	born,Animals
R6975	T9656	T9655	cc	and,born
R6976	T9657	T9655	conj	raised,born
R6977	T9658	T9657	prep	on,raised
R6978	T9659	T9658	pobj	dox,on
R6979	T9660	T9661	compound	activity,levels
R6986	T10541	T10542	nsubj	We,present
R6987	T10543	T10544	det	a,model
R6988	T10544	T10542	dobj	model,present
R6989	T10545	T10544	amod	new,model
R6990	T10546	T10544	compound	mouse,model
R6991	T10547	T10544	prep	for,model
R6992	T10548	T10547	pobj	AD,for
R6993	T10549	T10550	dep	that,designed
R6994	T10550	T10544	relcl	designed,model
R6995	T10551	T10550	auxpass	was,designed
R6996	T10552	T10553	aux	to,test
R6997	T10553	T10550	advcl	test,designed
R6998	T10554	T10555	det	the,consequences
R6999	T10555	T10553	dobj	consequences,test
R7000	T10556	T10555	prep	of,consequences
R7001	T10557	T10556	pcomp	inhibiting,of
R7002	T10558	T10559	compound	Aβ,production
R7003	T10559	T10557	dobj	production,inhibiting
R7004	T10560	T10557	prep	after,inhibiting
R7005	T10561	T10562	det	the,onset
R7006	T10562	T10560	pobj	onset,after
R7007	T10563	T10562	prep	of,onset
R7008	T10564	T10565	compound	amyloid,pathology
R7009	T10565	T10563	pobj	pathology,of
R7010	T10566	T10542	punct	.,present
R7011	T10568	T10569	amod	New,lines
R7012	T10569	T10570	nsubjpass	lines,developed
R7013	T10571	T10569	prep	of,lines
R7014	T10572	T10573	amod	transgenic,mice
R7015	T10573	T10571	pobj	mice,of
R7016	T10574	T10570	auxpass	were,developed
R7017	T10575	T10570	prep	for,developed
R7018	T10576	T10577	det	this,study
R7019	T10577	T10575	pobj	study,for
R7020	T10578	T10579	dep	that,express
R7021	T10579	T10570	ccomp	express,developed
R7022	T10580	T10581	amod	high,levels
R7023	T10581	T10579	dobj	levels,express
R7024	T10582	T10581	prep	of,levels
R7025	T10583	T10584	compound	APPswe,ind
R7026	T10584	T10582	pobj	ind,of
R7027	T10585	T10584	punct	/,ind
R7028	T10586	T10579	prep	under,express
R7029	T10587	T10588	det	the,control
R7030	T10588	T10586	pobj	control,under
R7031	T10589	T10588	prep	of,control
R7032	T10590	T10591	det	a,promoter
R7033	T10591	T10589	pobj	promoter,of
R7034	T10592	T10593	npadvmod	tetracycline,responsive
R7035	T10593	T10591	amod	responsive,promoter
R7036	T10594	T10593	punct	-,responsive
R7037	T10595	T10570	punct	.,developed
R7038	T10597	T10598	nsubj	We,demonstrate
R7039	T10599	T10600	mark	that,suppresses
R7040	T10600	T10598	ccomp	suppresses,demonstrate
R7041	T10601	T10600	nsubj	treatment,suppresses
R7042	T10602	T10601	prep	with,treatment
R7043	T10603	T10602	pobj	dox,with
R7044	T10604	T10605	amod	steady,state
R7045	T10605	T10607	compound	state,levels
R7046	T10606	T10605	punct	-,state
R7047	T10607	T10600	dobj	levels,suppresses
R7048	T10608	T10607	prep	of,levels
R7049	T10609	T10610	preconj	both,ind
R7050	T10610	T10608	pobj	ind,of
R7051	T10611	T10610	compound	APPswe,ind
R7052	T10612	T10610	punct	/,ind
R7053	T10613	T10610	cc	and,ind
R7054	T10614	T10615	poss	its,fragments
R7055	T10615	T10610	conj	fragments,ind
R7056	T10616	T10615	nmod	C,fragments
R7057	T10617	T10615	punct	-,fragments
R7058	T10618	T10615	amod	terminal,fragments
R7059	T10619	T10600	punct	", ",suppresses
R7060	T10620	T10600	advcl	indicating,suppresses
R7061	T10621	T10622	mark	that,have
R7062	T10622	T10620	ccomp	have,indicating
R7063	T10623	T10624	det	the,proteins
R7064	T10624	T10622	nsubj	proteins,have
R7065	T10625	T10624	compound	mutant,proteins
R7066	T10626	T10627	det	a,life
R7067	T10627	T10622	dobj	life,have
R7068	T10628	T10629	advmod	relatively,short
R7069	T10629	T10627	amod	short,life
R7070	T10630	T10627	compound	half,life
R7071	T10631	T10627	punct	-,life
R7072	T10632	T10633	advmod	in,vivo
R7073	T10633	T10622	advmod	vivo,have
R7074	T10634	T10598	punct	.,demonstrate
R7075	T10636	T10637	amod	Transgenic,expression
R7076	T10637	T10638	nsubj	expression,cause
R7077	T10639	T10637	prep	of,expression
R7078	T10640	T10641	compound	APPswe,ind
R7079	T10641	T10639	pobj	ind,of
R7080	T10642	T10641	punct	/,ind
R7081	T10643	T10637	cc	and,expression
R7082	T10644	T10645	amod	consequent,overproduction
R7083	T10645	T10637	conj	overproduction,expression
R7084	T10646	T10645	prep	of,overproduction
R7085	T10647	T10646	pobj	Aβ42,of
R7086	T10648	T10649	amod	early,onset
R7087	T10649	T10651	compound	onset,deposition
R7088	T10650	T10649	punct	-,onset
R7089	T10651	T10638	dobj	deposition,cause
R7090	T10652	T10651	compound	amyloid,deposition
R7091	T10653	T10638	prep	in,cause
R7092	T10654	T10655	amod	untreated,mice
R7093	T10655	T10653	pobj	mice,in
R7094	T10656	T10655	punct	", ",mice
R7095	T10657	T10658	prep	in,appear
R7096	T10658	T10655	relcl	appear,mice
R7097	T10659	T10657	pobj	which,in
R7098	T10660	T10658	nsubj	deposits,appear
R7099	T10661	T10662	advmod	as,early
R7100	T10662	T10658	advmod	early,appear
R7101	T10663	T10662	prep	as,early
R7102	T10664	T10665	nummod	2,mo
R7103	T10665	T10663	pobj	mo,as
R7104	T10666	T10665	prep	of,mo
R7105	T10667	T10666	pobj	age,of
R7106	T10668	T10638	punct	.,cause
R7107	T10670	T10671	compound	Amyloid,burden
R7108	T10671	T10672	nsubj	burden,worsens
R7109	T10673	T10672	advmod	significantly,worsens
R7110	T10674	T10672	prep	with,worsens
R7111	T10675	T10674	pobj	age,with
R7112	T10676	T10672	punct	", ",worsens
R7113	T10677	T10672	cc	and,worsens
R7114	T10678	T10679	prep	by,filled
R7115	T10679	T10672	conj	filled,worsens
R7116	T10680	T10681	nummod	9,mo
R7117	T10681	T10678	pobj	mo,by
R7118	T10682	T10679	punct	", ",filled
R7119	T10683	T10684	det	the,hippocampus
R7120	T10684	T10679	nsubjpass	hippocampus,filled
R7121	T10685	T10684	cc	and,hippocampus
R7122	T10686	T10684	conj	cortex,hippocampus
R7123	T10687	T10684	prep	of,hippocampus
R7124	T10688	T10689	amod	untreated,mice
R7125	T10689	T10687	pobj	mice,of
R7126	T10690	T10679	auxpass	are,filled
R7127	T10691	T10679	advmod	largely,filled
R7128	T10692	T10679	prep	with,filled
R7129	T10693	T10694	amod	aggregated,peptide
R7130	T10694	T10692	pobj	peptide,with
R7131	T10695	T10679	punct	.,filled
R7132	T10697	T10698	nsubj	We,find
R7133	T10699	T10700	mark	that,halts
R7134	T10700	T10698	ccomp	halts,find
R7135	T10701	T10700	nsubj	suppression,halts
R7136	T10702	T10701	prep	of,suppression
R7137	T10703	T10704	amod	transgenic,APP
R7138	T10704	T10702	pobj	APP,of
R7139	T10705	T10701	prep	by,suppression
R7140	T10706	T10707	amod	more,95
R7141	T10707	T10709	nummod	95,%
R7142	T10708	T10707	quantmod	than,95
R7143	T10709	T10705	pobj	%,by
R7144	T10710	T10700	advmod	abruptly,halts
R7145	T10711	T10712	det	the,progression
R7146	T10712	T10700	dobj	progression,halts
R7147	T10713	T10712	prep	of,progression
R7148	T10714	T10715	compound	amyloid,pathology
R7149	T10715	T10713	pobj	pathology,of
R7150	T10716	T10698	punct	.,find
R7151	T10718	T10719	advmod	Importantly,occurs
R7152	T10720	T10719	punct	", ",occurs
R7153	T10721	T10722	det	this,outcome
R7154	T10722	T10719	nsubj	outcome,occurs
R7155	T10723	T10719	prep	in,occurs
R7156	T10724	T10723	pobj	animals,in
R7157	T10725	T10726	advmod	already,harboring
R7158	T10726	T10724	acl	harboring,animals
R7159	T10727	T10728	amod	considerable,pathology
R7160	T10728	T10726	dobj	pathology,harboring
R7161	T10729	T10728	compound	amyloid,pathology
R7162	T10730	T10719	punct	", ",occurs
R7163	T10731	T10732	det	a,situation
R7164	T10732	T10719	npadvmod	situation,occurs
R7165	T10733	T10732	amod	similar,situation
R7166	T10734	T10733	prep	to,similar
R7167	T10735	T10736	dep	what,expected
R7168	T10736	T10734	pcomp	expected,to
R7169	T10737	T10736	aux	might,expected
R7170	T10738	T10736	auxpass	be,expected
R7171	T10739	T10736	prep	in,expected
R7172	T10740	T10739	pobj	patients,in
R7173	T10741	T10742	aux	to,treated
R7174	T10742	T10740	advcl	treated,patients
R7175	T10743	T10742	auxpass	be,treated
R7176	T10744	T10742	prep	with,treated
R7177	T10745	T10746	compound	secretase,inhibitors
R7178	T10746	T10744	pobj	inhibitors,with
R7179	T10747	T10719	punct	.,occurs
R7180	T10749	T10750	advmod	Somewhat,unexpectedly
R7181	T10750	T10751	advmod	unexpectedly,observe
R7182	T10752	T10751	punct	", ",observe
R7183	T10753	T10751	nsubj	we,observe
R7184	T10754	T10755	det	no,clearance
R7185	T10755	T10751	dobj	clearance,observe
R7186	T10756	T10755	amod	appreciable,clearance
R7187	T10757	T10755	prep	of,clearance
R7188	T10758	T10759	amod	deposited,amyloid
R7189	T10759	T10757	pobj	amyloid,of
R7190	T10760	T10761	advmod	even,following
R7191	T10761	T10751	prep	following,observe
R7192	T10762	T10761	pobj	periods,following
R7193	T10763	T10762	prep	of,periods
R7194	T10764	T10765	compound	transgene,suppression
R7195	T10765	T10763	pobj	suppression,of
R7196	T10766	T10762	amod	equal,periods
R7197	T10767	T10766	prep	to,equal
R7198	T10768	T10769	det	the,time
R7199	T10769	T10767	pobj	time,to
R7200	T10770	T10769	advcl	taken,time
R7201	T10771	T10772	mark	for,form
R7202	T10772	T10770	advcl	form,taken
R7203	T10773	T10772	nsubj	plaques,form
R7204	T10774	T10772	aux	to,form
R7205	T10775	T10751	punct	.,observe
R7206	T10777	T10778	det	This,finding
R7207	T10778	T10780	nsubj	finding,indicates
R7208	T10779	T10778	amod	latter,finding
R7209	T10781	T10782	mark	that,is
R7210	T10782	T10780	ccomp	is,indicates
R7211	T10783	T10782	prep	compared,is
R7212	T10784	T10783	prep	to,compared
R7213	T10785	T10786	amod	other,aggregates
R7214	T10786	T10784	pobj	aggregates,to
R7215	T10787	T10788	npadvmod	disease,associated
R7216	T10788	T10786	amod	associated,aggregates
R7217	T10789	T10788	punct	-,associated
R7218	T10790	T10786	compound	protein,aggregates
R7219	T10791	T10792	amod	such,as
R7220	T10792	T10786	prep	as,aggregates
R7221	T10793	T10794	compound	mutant,huntingtin
R7222	T10794	T10792	pobj	huntingtin,as
R7223	T10795	T10794	punct	", ",huntingtin
R7224	T10796	T10797	dep	which,clear
R7225	T10797	T10794	relcl	clear,huntingtin
R7226	T10798	T10797	prep	in,clear
R7227	T10799	T10800	amod	less,3
R7228	T10800	T10802	nummod	3,mo
R7229	T10801	T10800	quantmod	than,3
R7230	T10802	T10798	pobj	mo,in
R7231	T10803	T10804	punct	[,32
R7232	T10804	T10797	parataxis	32,clear
R7233	T10805	T10804	punct	],32
R7234	T10806	T10782	punct	", ",is
R7235	T10807	T10808	det	the,disaggregation
R7236	T10808	T10782	nsubj	disaggregation,is
R7237	T10809	T10808	prep	of,disaggregation
R7238	T10810	T10811	amod	extracellular,amyloid
R7239	T10811	T10809	pobj	amyloid,of
R7240	T10812	T10813	advmod	relatively,slow
R7241	T10813	T10782	acomp	slow,is
R7242	T10814	T10780	punct	.,indicates
R7243	T10816	T10817	advmod	Notably,show
R7244	T10818	T10817	punct	", ",show
R7245	T10819	T10820	amod	pharmaceutical,inhibitors
R7246	T10820	T10817	nsubj	inhibitors,show
R7247	T10821	T10822	punct	γ,secretase
R7248	T10822	T10820	compound	secretase,inhibitors
R7249	T10823	T10822	punct	-,secretase
R7250	T10824	T10820	acl	published,inhibitors
R7251	T10825	T10824	prep	to,published
R7252	T10826	T10825	pobj	date,to
R7253	T10827	T10828	advmod	less,strict
R7254	T10828	T10829	amod	strict,regulation
R7255	T10829	T10817	dobj	regulation,show
R7256	T10830	T10829	prep	of,regulation
R7257	T10831	T10832	compound	Aβ,production
R7258	T10832	T10830	pobj	production,of
R7259	T10833	T10829	prep	following,regulation
R7260	T10834	T10835	amod	chronic,administration
R7261	T10835	T10833	pobj	administration,following
R7262	T10836	T10837	mark	than,attained
R7263	T10837	T10829	advcl	attained,regulation
R7264	T10838	T10837	nsubj	we,attained
R7265	T10839	T10837	aux	have,attained
R7266	T10840	T10837	advmod	here,attained
R7267	T10841	T10817	punct	.,show
R7268	T10843	T10844	nummod	Two,compounds
R7269	T10844	T10846	nsubj	compounds,show
R7270	T10845	T10844	amod	independent,compounds
R7271	T10847	T10844	acl	tested,compounds
R7272	T10848	T10847	prep	in,tested
R7273	T10849	T10850	npadvmod	Tg2576,transgenic
R7274	T10850	T10859	amod	transgenic,mice
R7275	T10851	T10852	punct	[,33
R7276	T10852	T10849	parataxis	33,Tg2576
R7277	T10853	T10852	punct	],33
R7278	T10854	T10849	cc	and,Tg2576
R7279	T10855	T10849	conj	TgCRND8,Tg2576
R7280	T10856	T10857	punct	[,34
R7281	T10857	T10855	parataxis	34,TgCRND8
R7282	T10858	T10857	punct	],34
R7283	T10859	T10848	pobj	mice,in
R7284	T10860	T10861	neg	no,85
R7285	T10861	T10864	nummod	85,%
R7286	T10862	T10861	amod	more,85
R7287	T10863	T10861	quantmod	than,85
R7288	T10864	T10865	compound	%,suppression
R7289	T10865	T10846	dobj	suppression,show
R7290	T10866	T10865	prep	of,suppression
R7291	T10867	T10868	compound	Aβ40,levels
R7292	T10868	T10866	pobj	levels,of
R7293	T10869	T10865	punct	", ",suppression
R7294	T10870	T10865	cc	and,suppression
R7295	T10871	T10872	advmod	where,measured
R7296	T10872	T10873	advcl	measured,suppression
R7297	T10873	T10865	conj	suppression,suppression
R7298	T10874	T10873	punct	", ",suppression
R7299	T10875	T10876	advmod	even,less
R7300	T10876	T10873	amod	less,suppression
R7301	T10877	T10873	prep	of,suppression
R7302	T10878	T10877	pobj	Aβ42,of
R7303	T10879	T10880	punct	(,%
R7304	T10880	T10878	parataxis	%,Aβ42
R7305	T10881	T10882	advmod	approximately,60
R7306	T10882	T10880	nummod	60,%
R7307	T10883	T10880	punct	),%
R7308	T10884	T10885	punct	[,36
R7309	T10885	T10846	parataxis	36,show
R7310	T10886	T10885	nummod	35,36
R7311	T10887	T10885	punct	",",36
R7312	T10888	T10885	punct	],36
R7313	T10889	T10846	punct	.,show
R7314	T10891	T10892	advmod	Thus,achieved
R7315	T10893	T10892	punct	", ",achieved
R7316	T10894	T10895	advmod	even,after
R7317	T10895	T10892	prep	after,achieved
R7318	T10896	T10895	pcomp	accounting,after
R7319	T10897	T10896	prep	for,accounting
R7320	T10898	T10899	det	the,levels
R7321	T10899	T10897	pobj	levels,for
R7322	T10900	T10901	advmod	much,higher
R7323	T10901	T10899	amod	higher,levels
R7324	T10902	T10899	compound	APP,levels
R7325	T10903	T10899	compound	expression,levels
R7326	T10904	T10899	prep	in,levels
R7327	T10905	T10906	poss	our,mice
R7328	T10906	T10904	pobj	mice,in
R7329	T10907	T10899	prep	than,levels
R7330	T10908	T10907	prep	in,than
R7331	T10909	T10910	det	the,lines
R7332	T10910	T10908	pobj	lines,in
R7333	T10911	T10910	nmod	Tg2576,lines
R7334	T10912	T10911	cc	and,Tg2576
R7335	T10913	T10911	conj	TgCRND8,Tg2576
R7336	T10914	T10892	punct	", ",achieved
R7337	T10915	T10892	nsubj	we,achieved
R7338	T10916	T10892	aux	have,achieved
R7339	T10917	T10918	advmod	better,suppression
R7340	T10918	T10892	dobj	suppression,achieved
R7341	T10919	T10918	amod	absolute,suppression
R7342	T10920	T10918	prep	of,suppression
R7343	T10921	T10922	compound	Aβ,production
R7344	T10922	T10920	pobj	production,of
R7345	T10923	T10918	prep	with,suppression
R7346	T10924	T10925	det	the,system
R7347	T10925	T10923	pobj	system,with
R7348	T10926	T10927	compound	tet,off
R7349	T10927	T10925	compound	off,system
R7350	T10928	T10927	punct	-,off
R7351	T10929	T10930	mark	than,is
R7352	T10930	T10918	advcl	is,suppression
R7353	T10931	T10930	advmod	currently,is
R7354	T10932	T10930	acomp	possible,is
R7355	T10933	T10930	prep	with,is
R7356	T10934	T10935	amod	published,inhibitors
R7357	T10935	T10933	pobj	inhibitors,with
R7358	T10936	T10937	punct	γ,secretase
R7359	T10937	T10935	compound	secretase,inhibitors
R7360	T10938	T10937	punct	-,secretase
R7361	T10939	T10892	punct	.,achieved
R7362	T10941	T10942	mark	Since,are
R7363	T10942	T10950	advcl	are,provides
R7364	T10943	T10944	advmod	even,agents
R7365	T10944	T10942	nsubj	agents,are
R7366	T10945	T10944	det	the,agents
R7367	T10946	T10947	advmod	most,advanced
R7368	T10947	T10944	amod	advanced,agents
R7369	T10948	T10944	amod	future,agents
R7370	T10949	T10944	amod	pharmaceutical,agents
R7371	T10951	T10942	acomp	unlikely,are
R7372	T10952	T10953	aux	to,attain
R7373	T10953	T10951	xcomp	attain,unlikely
R7374	T10954	T10955	advmod	more,complete
R7375	T10955	T10956	amod	complete,control
R7376	T10956	T10953	dobj	control,attain
R7377	T10957	T10956	prep	of,control
R7378	T10958	T10959	compound	Aβ,production
R7379	T10959	T10957	pobj	production,of
R7380	T10960	T10961	mark	than,achieved
R7381	T10961	T10956	advcl	achieved,control
R7382	T10962	T10961	advmod	here,achieved
R7383	T10963	T10950	punct	", ",provides
R7384	T10964	T10965	det	this,system
R7385	T10965	T10950	nsubj	system,provides
R7386	T10966	T10967	det	a,test
R7387	T10967	T10950	dobj	test,provides
R7388	T10968	T10967	amod	salient,test
R7389	T10969	T10967	prep	of,test
R7390	T10970	T10971	amod	therapeutic,intervention
R7391	T10971	T10969	pobj	intervention,of
R7392	T10972	T10971	prep	with,intervention
R7393	T10973	T10974	npadvmod	Aβ,lowering
R7394	T10974	T10976	amod	lowering,compounds
R7395	T10975	T10974	punct	-,lowering
R7396	T10976	T10972	pobj	compounds,with
R7397	T10977	T10950	punct	.,provides
R7398	T10979	T10980	mark	Although,arrested
R7399	T10980	T10988	advcl	arrested,found
R7400	T10981	T10982	det	the,progression
R7401	T10982	T10980	nsubjpass	progression,arrested
R7402	T10983	T10982	prep	of,progression
R7403	T10984	T10985	compound	amyloid,deposition
R7404	T10985	T10983	pobj	deposition,of
R7405	T10986	T10980	auxpass	was,arrested
R7406	T10987	T10980	advmod	sharply,arrested
R7407	T10989	T10980	agent	by,arrested
R7408	T10990	T10991	det	this,approach
R7409	T10991	T10989	pobj	approach,by
R7410	T10992	T10988	punct	", ",found
R7411	T10993	T10988	nsubj	we,found
R7412	T10994	T10995	mark	that,remained
R7413	T10995	T10988	ccomp	remained,found
R7414	T10996	T10997	det	a,burden
R7415	T10997	T10995	nsubj	burden,remained
R7416	T10998	T10997	amod	substantial,burden
R7417	T10999	T10997	compound	amyloid,burden
R7418	T11000	T11001	advmod	even,after
R7419	T11001	T10995	prep	after,remained
R7420	T11002	T11003	amod	long,periods
R7421	T11003	T11001	pobj	periods,after
R7422	T11004	T11003	prep	of,periods
R7423	T11005	T11006	compound	transgene,suppression
R7424	T11006	T11004	pobj	suppression,of
R7425	T11007	T10988	punct	.,found
R7426	T11009	T11010	nsubj	We,examined
R7427	T11011	T11012	det	a,number
R7428	T11012	T11010	dobj	number,examined
R7429	T11013	T11012	amod	small,number
R7430	T11014	T11012	prep	of,number
R7431	T11015	T11014	pobj	animals,of
R7432	T11016	T11010	prep	after,examined
R7433	T11017	T11018	nummod	12,mo
R7434	T11018	T11016	pobj	mo,after
R7435	T11019	T11018	prep	of,mo
R7436	T11020	T11021	compound	dox,treatment
R7437	T11021	T11019	pobj	treatment,of
R7438	T11022	T11021	punct	(,treatment
R7439	T11023	T11021	acl	beginning,treatment
R7440	T11024	T11023	prep	at,beginning
R7441	T11025	T11026	nummod	6,mo
R7442	T11026	T11024	pobj	mo,at
R7443	T11027	T11026	prep	of,mo
R7444	T11028	T11027	pobj	age,of
R7445	T11029	T11021	punct	),treatment
R7446	T11030	T11010	punct	", ",examined
R7447	T11031	T11010	cc	and,examined
R7448	T11032	T11010	conj	found,examined
R7449	T11033	T11034	mark	that,were
R7450	T11034	T11032	ccomp	were,found
R7451	T11035	T11036	compound	amyloid,deposits
R7452	T11036	T11034	nsubj	deposits,were
R7453	T11037	T11034	advmod	still,were
R7454	T11038	T11039	advmod	relatively,abundant
R7455	T11039	T11034	acomp	abundant,were
R7456	T11040	T11010	punct	.,examined
R7457	T11042	T11043	amod	Longer,term
R7458	T11043	T11045	compound	term,treatments
R7459	T11044	T11043	punct	-,term
R7460	T11045	T11046	nsubj	treatments,are
R7461	T11047	T11046	advmod	now,are
R7462	T11048	T11046	prep	in,are
R7463	T11049	T11048	pobj	progress,in
R7464	T11050	T11046	punct	.,are
R7465	T11052	T11053	prep	At,showed
R7466	T11054	T11055	det	the,interval
R7467	T11055	T11052	pobj	interval,At
R7468	T11056	T11055	amod	latest,interval
R7469	T11057	T11055	compound	treatment,interval
R7470	T11058	T11055	acl	analyzed,interval
R7471	T11059	T11058	agent	by,analyzed
R7472	T11060	T11059	pobj	ELISA,by
R7473	T11061	T11053	punct	", ",showed
R7474	T11062	T11053	nsubj	animals,showed
R7475	T11063	T11062	acl	administered,animals
R7476	T11064	T11063	dobj	dox,administered
R7477	T11065	T11063	prep	for,administered
R7478	T11066	T11067	nummod	6,mo
R7479	T11067	T11065	pobj	mo,for
R7480	T11068	T11069	amod	elevated,levels
R7481	T11069	T11053	dobj	levels,showed
R7482	T11070	T11069	prep	of,levels
R7483	T11071	T11072	npadvmod	PBS,soluble
R7484	T11072	T11074	amod	soluble,Aβ
R7485	T11073	T11072	punct	-,soluble
R7486	T11074	T11070	pobj	Aβ,of
R7487	T11075	T11076	punct	(,see
R7488	T11076	T11074	parataxis	see,Aβ
R7489	T11077	T11076	dobj	Figure,see
R7490	T11078	T11077	nummod	5,Figure
R7491	T11079	T11076	punct	),see
R7492	T11080	T11081	dep	that,interpreted
R7493	T11081	T11069	relcl	interpreted,levels
R7494	T11082	T11081	aux	could,interpreted
R7495	T11083	T11081	auxpass	be,interpreted
R7496	T11084	T11081	prep	as,interpreted
R7497	T11085	T11086	det	an,indication
R7498	T11086	T11084	pobj	indication,as
R7499	T11087	T11088	mark	that,releasing
R7500	T11088	T11086	acl	releasing,indication
R7501	T11089	T11090	det	the,plaques
R7502	T11090	T11088	nsubj	plaques,releasing
R7503	T11091	T11088	aux	are,releasing
R7504	T11092	T11088	advmod	slowly,releasing
R7505	T11093	T11088	dobj	peptide,releasing
R7506	T11094	T11093	punct	(,peptide
R7507	T11095	T11093	cc	or,peptide
R7508	T11096	T11097	amod	oligomeric,Aβ
R7509	T11097	T11093	conj	Aβ,peptide
R7510	T11098	T11097	punct	),Aβ
R7511	T11099	T11088	prep	into,releasing
R7512	T11100	T11101	det	the,pool
R7513	T11101	T11099	pobj	pool,into
R7514	T11102	T11101	amod	soluble,pool
R7515	T11103	T11088	cc	and,releasing
R7516	T11104	T11105	aux	might,dissolve
R7517	T11105	T11088	conj	dissolve,releasing
R7518	T11106	T11105	advmod	eventually,dissolve
R7519	T11107	T11053	punct	.,showed
R7520	T11109	T11110	mark	Whether,result
R7521	T11110	T11124	csubj	result,is
R7522	T11111	T11110	csubj	inhibiting,result
R7523	T11112	T11113	compound	Aβ,production
R7524	T11113	T11111	dobj	production,inhibiting
R7525	T11114	T11111	advmod	longer,inhibiting
R7526	T11115	T11114	prep	than,longer
R7527	T11116	T11117	nummod	6,mo
R7528	T11117	T11115	pobj	mo,than
R7529	T11118	T11116	punct	(,6
R7530	T11119	T11116	cc	or,6
R7531	T11120	T11116	conj	12,6
R7532	T11121	T11117	punct	),mo
R7533	T11122	T11110	aux	may,result
R7534	T11123	T11110	advmod	ultimately,result
R7535	T11124	T11129	ccomp	is,limits
R7536	T11125	T11110	prep	in,result
R7537	T11126	T11125	pobj	clearance,in
R7538	T11127	T11126	prep	of,clearance
R7539	T11128	T11127	pobj	amyloid,of
R7540	T11130	T11124	prep	under,is
R7541	T11131	T11130	pobj	investigation,under
R7542	T11132	T11129	punct	;,limits
R7543	T11133	T11129	advmod	unfortunately,limits
R7544	T11134	T11129	punct	", ",limits
R7545	T11135	T11136	det	the,span
R7546	T11136	T11129	nsubj	span,limits
R7547	T11137	T11136	compound	life,span
R7548	T11138	T11136	prep	of,span
R7549	T11139	T11140	det	the,model
R7550	T11140	T11138	pobj	model,of
R7551	T11141	T11129	advmod	eventually,limits
R7552	T11142	T11143	det	this,experiment
R7553	T11143	T11129	dobj	experiment,limits
R7554	T11144	T11129	punct	.,limits
R7555	T11146	T11147	nsubj	Therapeutics,have
R7556	T11148	T11146	acl	used,Therapeutics
R7557	T11149	T11148	prep	in,used
R7558	T11150	T11149	pobj	humans,in
R7559	T11151	T11147	aux	will,have
R7560	T11152	T11153	advmod	considerably,more
R7561	T11153	T11154	amod	more,time
R7562	T11154	T11147	dobj	time,have
R7563	T11155	T11156	aux	to,act
R7564	T11156	T11154	advcl	act,time
R7565	T11157	T11158	mark	than,is
R7566	T11158	T11154	advcl	is,time
R7567	T11159	T11158	acomp	possible,is
R7568	T11160	T11158	prep	within,is
R7569	T11161	T11162	det	the,span
R7570	T11162	T11160	pobj	span,within
R7571	T11163	T11162	compound	life,span
R7572	T11164	T11162	prep	of,span
R7573	T11165	T11166	compound	rodent,models
R7574	T11166	T11164	pobj	models,of
R7575	T11167	T11147	punct	.,have
R7576	T11169	T11170	amod	Long,term
R7577	T11170	T11172	compound	term,treatments
R7578	T11171	T11170	punct	-,term
R7579	T11172	T11173	nsubj	treatments,be
R7580	T11174	T11173	aux	would,be
R7581	T11175	T11173	advmod	certainly,be
R7582	T11176	T11173	acomp	possible,be
R7583	T11177	T11173	cc	and,be
R7584	T11178	T11179	aux	could,be
R7585	T11179	T11173	conj	be,be
R7586	T11180	T11181	det	a,key
R7587	T11181	T11179	attr	key,be
R7588	T11182	T11181	prep	to,key
R7589	T11183	T11184	amod	effective,therapy
R7590	T11184	T11182	pobj	therapy,to
R7591	T11185	T11173	punct	.,be
R7592	T11187	T11188	advmod	Overall,interpret
R7593	T11189	T11188	punct	", ",interpret
R7594	T11190	T11188	advmod	however,interpret
R7595	T11191	T11188	punct	", ",interpret
R7596	T11192	T11188	nsubj	we,interpret
R7597	T11193	T11194	poss	our,findings
R7598	T11194	T11188	dobj	findings,interpret
R7599	T11195	T11188	prep	as,interpret
R7600	T11196	T11195	pobj	evidence,as
R7601	T11197	T11198	mark	that,reverse
R7602	T11198	T11196	acl	reverse,evidence
R7603	T11199	T11200	compound	AD,therapies
R7604	T11200	T11198	nsubj	therapies,reverse
R7605	T11201	T11202	dep	that,lower
R7606	T11202	T11200	relcl	lower,therapies
R7607	T11203	T11202	advmod	significantly,lower
R7608	T11204	T11205	det	the,production
R7609	T11205	T11202	dobj	production,lower
R7610	T11206	T11205	prep	of,production
R7611	T11207	T11206	pobj	Aβ,of
R7612	T11208	T11202	punct	(,lower
R7613	T11209	T11202	prep	by,lower
R7614	T11210	T11211	preconj	either,inhibiting
R7615	T11211	T11209	pcomp	inhibiting,by
R7616	T11212	T11213	compound	secretase,activity
R7617	T11213	T11211	dobj	activity,inhibiting
R7618	T11214	T11211	cc	or,inhibiting
R7619	T11215	T11211	conj	inhibiting,inhibiting
R7620	T11216	T11217	compound	APP,expression
R7621	T11217	T11215	dobj	expression,inhibiting
R7622	T11218	T11202	punct	),lower
R7623	T11219	T11198	aux	may,reverse
R7624	T11220	T11198	neg	not,reverse
R7625	T11221	T11198	advmod	quickly,reverse
R7626	T11222	T11223	amod	preexisting,pathology
R7627	T11223	T11198	dobj	pathology,reverse
R7628	T11224	T11198	punct	", ",reverse
R7629	T11225	T11198	cc	but,reverse
R7630	T11226	T11227	aux	should,halt
R7631	T11227	T11198	conj	halt,reverse
R7632	T11228	T11227	advmod	effectively,halt
R7633	T11229	T11230	amod	further,deposition
R7634	T11230	T11227	dobj	deposition,halt
R7635	T11231	T11230	prep	of,deposition
R7636	T11232	T11231	pobj	amyloid,of
R7637	T11233	T11188	punct	.,interpret
R7638	T11235	T11236	prep	In,remembered
R7639	T11237	T11235	pcomp	interpreting,In
R7640	T11238	T11239	poss	our,study
R7641	T11239	T11237	dobj	study,interpreting
R7642	T11240	T11236	punct	", ",remembered
R7643	T11241	T11236	nsubjpass	it,remembered
R7644	T11242	T11236	aux	should,remembered
R7645	T11243	T11236	auxpass	be,remembered
R7646	T11244	T11245	mark	that,are
R7647	T11245	T11236	ccomp	are,remembered
R7648	T11246	T11247	det	the,plaques
R7649	T11247	T11245	nsubj	plaques,are
R7650	T11248	T11247	amod	earliest,plaques
R7651	T11249	T11250	aux	to,appear
R7652	T11250	T11247	advcl	appear,plaques
R7653	T11251	T11250	prep	in,appear
R7654	T11252	T11253	det	these,mice
R7655	T11253	T11251	pobj	mice,in
R7656	T11254	T11247	punct	", ",plaques
R7657	T11255	T11247	prep	like,plaques
R7658	T11256	T11257	amod	other,transgenics
R7659	T11257	T11255	pobj	transgenics,like
R7660	T11258	T11257	compound	APP,transgenics
R7661	T11259	T11257	acl	harboring,transgenics
R7662	T11260	T11261	det	the,mutation
R7663	T11261	T11259	dobj	mutation,harboring
R7664	T11262	T11261	amod	Swedish,mutation
R7665	T11263	T11264	punct	[,27
R7666	T11264	T11259	parataxis	27,harboring
R7667	T11265	T11264	punct	],27
R7668	T11266	T11245	punct	", ",are
R7669	T11267	T11245	advmod	predominantly,are
R7670	T11268	T11269	amod	fibrillar,deposits
R7671	T11269	T11245	attr	deposits,are
R7672	T11270	T11269	punct	", ",deposits
R7673	T11271	T11272	dep	which,be
R7674	T11272	T11269	relcl	be,deposits
R7675	T11273	T11272	aux	may,be
R7676	T11274	T11275	advmod	less,tractable
R7677	T11275	T11272	acomp	tractable,be
R7678	T11276	T11275	prep	than,tractable
R7679	T11277	T11278	det	the,aggregates
R7680	T11278	T11276	pobj	aggregates,than
R7681	T11279	T11278	amod	diffuse,aggregates
R7682	T11280	T11278	acl	thought,aggregates
R7683	T11281	T11282	aux	to,come
R7684	T11282	T11280	xcomp	come,thought
R7685	T11283	T11282	advmod	first,come
R7686	T11284	T11282	prep	in,come
R7687	T11285	T11286	det	the,course
R7688	T11286	T11284	pobj	course,in
R7689	T11287	T11286	prep	of,course
R7690	T11288	T11289	det	the,disease
R7691	T11289	T11287	pobj	disease,of
R7692	T11290	T11289	amod	human,disease
R7693	T11291	T11236	punct	.,remembered
R7694	T11293	T11294	advmod	However,suggest
R7695	T11295	T11294	punct	", ",suggest
R7696	T11296	T11297	poss	our,data
R7697	T11297	T11294	nsubj	data,suggest
R7698	T11298	T11299	mark	that,cleared
R7699	T11299	T11294	ccomp	cleared,suggest
R7700	T11300	T11301	mark	once,formed
R7701	T11301	T11299	advcl	formed,cleared
R7702	T11302	T11303	amod	diffuse,deposits
R7703	T11303	T11301	nsubjpass	deposits,formed
R7704	T11304	T11301	auxpass	are,formed
R7705	T11305	T11301	prep	in,formed
R7706	T11306	T11307	det	these,mice
R7707	T11307	T11305	pobj	mice,in
R7708	T11308	T11299	punct	", ",cleared
R7709	T11309	T11299	nsubjpass	they,cleared
R7710	T11310	T11299	auxpass	are,cleared
R7711	T11311	T11312	neg	no,more
R7712	T11312	T11313	advmod	more,easily
R7713	T11313	T11299	advmod	easily,cleared
R7714	T11314	T11299	prep	in,cleared
R7715	T11315	T11316	poss	our,system
R7716	T11316	T11314	pobj	system,in
R7717	T11317	T11299	prep	than,cleared
R7718	T11318	T11319	amod	cored,plaques
R7719	T11319	T11317	pobj	plaques,than
R7720	T11320	T11321	punct	(,see
R7721	T11321	T11299	parataxis	see,cleared
R7722	T11322	T11323	compound	Figure,S4
R7723	T11323	T11321	dobj	S4,see
R7724	T11324	T11321	punct	),see
R7725	T11325	T11294	punct	.,suggest
R7726	T11327	T11328	det	An,consideration
R7727	T11328	T11330	nsubj	consideration,is
R7728	T11329	T11328	amod	additional,consideration
R7729	T11331	T11332	nsubj	we,recognize
R7730	T11332	T11328	advcl	recognize,consideration
R7731	T11333	T11334	mark	that,continues
R7732	T11334	T11330	advcl	continues,is
R7733	T11335	T11336	det	a,amount
R7734	T11336	T11334	nsubj	amount,continues
R7735	T11337	T11336	amod	small,amount
R7736	T11338	T11336	prep	of,amount
R7737	T11339	T11340	compound	transgene,expression
R7738	T11340	T11338	pobj	expression,of
R7739	T11341	T11334	prep	in,continues
R7740	T11342	T11343	det	the,presence
R7741	T11343	T11341	pobj	presence,in
R7742	T11344	T11343	prep	of,presence
R7743	T11345	T11344	pobj	dox,of
R7744	T11346	T11334	cc	and,continues
R7745	T11347	T11348	mark	that,continues
R7746	T11348	T11334	conj	continues,continues
R7747	T11349	T11350	amod	endogenous,Aβ
R7748	T11350	T11348	nsubj	Aβ,continues
R7749	T11351	T11350	compound	mouse,Aβ
R7750	T11352	T11353	aux	to,produced
R7751	T11353	T11348	xcomp	produced,continues
R7752	T11354	T11353	auxpass	be,produced
R7753	T11355	T11330	punct	.,is
R7754	T11357	T11358	nsubj	It,is
R7755	T11359	T11358	acomp	possible,is
R7756	T11360	T11361	mark	that,are
R7757	T11361	T11358	ccomp	are,is
R7758	T11362	T11363	det	the,levels
R7759	T11363	T11361	nsubj	levels,are
R7760	T11364	T11363	amod	combined,levels
R7761	T11365	T11363	amod	low,levels
R7762	T11366	T11363	prep	of,levels
R7763	T11367	T11368	amod	endogenous,Aβ
R7764	T11368	T11370	nmod	Aβ,peptide
R7765	T11369	T11368	nmod	mouse,Aβ
R7766	T11370	T11366	pobj	peptide,of
R7767	T11371	T11368	cc	and,Aβ
R7768	T11372	T11373	amod	nonsuppressed,human
R7769	T11373	T11368	conj	human,Aβ
R7770	T11374	T11361	acomp	sufficient,are
R7771	T11375	T11376	aux	to,maintain
R7772	T11376	T11374	xcomp	maintain,sufficient
R7773	T11377	T11378	amod	existing,deposits
R7774	T11378	T11376	dobj	deposits,maintain
R7775	T11379	T11358	punct	.,is
R7776	T11381	T11382	advmod	However,are
R7777	T11383	T11382	punct	", ",are
R7778	T11384	T11385	det	these,levels
R7779	T11385	T11382	nsubj	levels,are
R7780	T11386	T11385	amod	low,levels
R7781	T11387	T11385	prep	of,levels
R7782	T11388	T11387	pobj	peptide,of
R7783	T11389	T11382	neg	not,are
R7784	T11390	T11382	acomp	sufficient,are
R7785	T11391	T11392	aux	to,induce
R7786	T11392	T11390	xcomp	induce,sufficient
R7787	T11393	T11394	amod	new,formation
R7788	T11394	T11392	dobj	formation,induce
R7789	T11395	T11394	compound	amyloid,formation
R7790	T11396	T11382	punct	", ",are
R7791	T11397	T11398	mark	as,develop
R7792	T11398	T11382	advcl	develop,are
R7793	T11399	T11400	nmod	CaMKIIα,ind
R7794	T11400	T11406	compound	ind,mice
R7795	T11401	T11400	punct	-,ind
R7796	T11402	T11400	nmod	tTA,ind
R7797	T11403	T11400	punct	×,ind
R7798	T11404	T11400	compound	tetAPPswe,ind
R7799	T11405	T11400	punct	/,ind
R7800	T11406	T11398	nsubj	mice,develop
R7801	T11407	T11406	acl	raised,mice
R7802	T11408	T11407	prep	on,raised
R7803	T11409	T11408	pobj	dox,on
R7804	T11410	T11407	prep	for,raised
R7805	T11411	T11412	quantmod	up,a
R7806	T11412	T11414	nummod	a,year
R7807	T11413	T11412	quantmod	to,a
R7808	T11414	T11410	pobj	year,for
R7809	T11415	T11398	aux	do,develop
R7810	T11416	T11398	neg	not,develop
R7811	T11417	T11418	compound	amyloid,lesions
R7812	T11418	T11398	dobj	lesions,develop
R7813	T11419	T11420	punct	(,shown
R7814	T11420	T11398	parataxis	shown,develop
R7815	T11421	T11420	nsubj	data,shown
R7816	T11422	T11420	neg	not,shown
R7817	T11423	T11420	punct	),shown
R7818	T11424	T11382	punct	.,are
R7819	T11426	T11427	nsubj	It,is
R7820	T11428	T11427	advmod	also,is
R7821	T11429	T11427	acomp	clear,is
R7822	T11430	T11431	mark	that,raised
R7823	T11431	T11427	ccomp	raised,is
R7824	T11432	T11431	prep	in,raised
R7825	T11433	T11434	det	this,system
R7826	T11434	T11432	pobj	system,in
R7827	T11435	T11434	amod	genetic,system
R7828	T11436	T11431	punct	", ",raised
R7829	T11437	T11431	nsubj	we,raised
R7830	T11438	T11431	aux	have,raised
R7831	T11439	T11440	det	the,production
R7832	T11440	T11431	dobj	production,raised
R7833	T11441	T11440	prep	of,production
R7834	T11442	T11441	pobj	Aβ,of
R7835	T11443	T11431	prep	to,raised
R7836	T11444	T11443	pobj	levels,to
R7837	T11445	T11446	neg	not,found
R7838	T11446	T11444	acl	found,levels
R7839	T11447	T11446	prep	in,found
R7840	T11448	T11447	pobj	humans,in
R7841	T11449	T11450	aux	to,accelerate
R7842	T11450	T11431	advcl	accelerate,raised
R7843	T11451	T11450	dobj	pathology,accelerate
R7844	T11452	T11450	prep	into,accelerate
R7845	T11453	T11454	det	an,frame
R7846	T11454	T11452	pobj	frame,into
R7847	T11455	T11456	advmod	experimentally,feasible
R7848	T11456	T11454	amod	feasible,frame
R7849	T11457	T11454	compound	time,frame
R7850	T11458	T11427	punct	.,is
R7851	T11460	T11461	det	This,system
R7852	T11461	T11462	nsubj	system,allowed
R7853	T11462	T11463	ccomp	allowed,favored
R7854	T11464	T11465	nsubj	us,create
R7855	T11465	T11462	ccomp	create,allowed
R7856	T11466	T11465	aux	to,create
R7857	T11467	T11468	det	an,differential
R7858	T11468	T11465	dobj	differential,create
R7859	T11469	T11470	advmod	approximately,20-fold
R7860	T11470	T11468	amod	20-fold,differential
R7861	T11471	T11468	prep	between,differential
R7862	T11472	T11473	nmod	APP,Aβ
R7863	T11473	T11475	nmod	Aβ,rates
R7864	T11474	T11473	punct	/,Aβ
R7865	T11475	T11471	pobj	rates,between
R7866	T11476	T11475	amod	synthetic,rates
R7867	T11477	T11465	prep	before,create
R7868	T11478	T11477	cc	and,before
R7869	T11479	T11477	conj	after,before
R7870	T11480	T11479	pobj	treatment,after
R7871	T11481	T11463	punct	", ",favored
R7872	T11482	T11463	advmod	yet,favored
R7873	T11483	T11484	det	the,equilibrium
R7874	T11484	T11463	nsubj	equilibrium,favored
R7875	T11485	T11486	advmod	in,vivo
R7876	T11486	T11484	amod	vivo,equilibrium
R7877	T11487	T11484	prep	between,equilibrium
R7878	T11488	T11489	amod	aggregated,states
R7879	T11489	T11487	pobj	states,between
R7880	T11490	T11488	cc	and,aggregated
R7881	T11491	T11488	conj	disaggregated,aggregated
R7882	T11492	T11489	prep	of,states
R7883	T11493	T11492	pobj	Aβ,of
R7884	T11494	T11463	advmod	still,favored
R7885	T11495	T11496	det	the,maintenance
R7886	T11496	T11463	dobj	maintenance,favored
R7887	T11497	T11496	prep	of,maintenance
R7888	T11498	T11499	compound	amyloid,deposits
R7889	T11499	T11497	pobj	deposits,of
R7890	T11500	T11463	punct	.,favored
R7891	T11502	T11503	prep	In,seems
R7892	T11504	T11505	poss	our,opinion
R7893	T11505	T11502	pobj	opinion,In
R7894	T11506	T11503	punct	", ",seems
R7895	T11507	T11503	nsubj	it,seems
R7896	T11508	T11503	oprd	unlikely,seems
R7897	T11509	T11510	mark	that,be
R7898	T11510	T11503	ccomp	be,seems
R7899	T11511	T11512	compound	amyloid,deposits
R7900	T11512	T11510	nsubj	deposits,be
R7901	T11513	T11512	prep	in,deposits
R7902	T11514	T11515	amod	human,brain
R7903	T11515	T11513	pobj	brain,in
R7904	T11516	T11510	aux	would,be
R7905	T11517	T11510	advmod	inherently,be
R7906	T11518	T11519	advmod	any,stable
R7907	T11519	T11510	acomp	stable,be
R7908	T11520	T11519	advmod	less,stable
R7909	T11521	T11519	prep	than,stable
R7910	T11522	T11521	pobj	those,than
R7911	T11523	T11522	acl	formed,those
R7912	T11524	T11523	prep	in,formed
R7913	T11525	T11526	compound	mouse,brain
R7914	T11526	T11524	pobj	brain,in
R7915	T11527	T11503	punct	.,seems
R7916	T11529	T11530	advmod	However,harbor
R7917	T11531	T11530	punct	", ",harbor
R7918	T11532	T11533	det	the,brain
R7919	T11533	T11530	nsubj	brain,harbor
R7920	T11534	T11533	amod	human,brain
R7921	T11535	T11530	aux	may,harbor
R7922	T11536	T11537	compound	clearance,mechanisms
R7923	T11537	T11530	dobj	mechanisms,harbor
R7924	T11538	T11539	neg	not,shared
R7925	T11539	T11537	acl	shared,mechanisms
R7926	T11540	T11539	agent	by,shared
R7927	T11541	T11540	pobj	mice,by
R7928	T11542	T11543	dep	that,allow
R7929	T11543	T11537	relcl	allow,mechanisms
R7930	T11544	T11543	aux	would,allow
R7931	T11545	T11546	advmod	more,efficient
R7932	T11546	T11547	amod	efficient,removal
R7933	T11547	T11543	dobj	removal,allow
R7934	T11548	T11547	prep	of,removal
R7935	T11549	T11550	amod	preexisting,amyloid
R7936	T11550	T11548	pobj	amyloid,of
R7937	T11551	T11530	punct	.,harbor
R7938	T11553	T11554	nummod	One,mechanism
R7939	T11554	T11556	nsubj	mechanism,is
R7940	T11555	T11554	amod	potential,mechanism
R7941	T11557	T11558	prep	by,removed
R7942	T11558	T11554	relcl	removed,mechanism
R7943	T11559	T11557	pobj	which,by
R7944	T11560	T11558	nsubjpass	amyloid,removed
R7945	T11561	T11558	aux	may,removed
R7946	T11562	T11558	auxpass	be,removed
R7947	T11563	T11564	advmod	more,efficiently
R7948	T11564	T11558	advmod	efficiently,removed
R7949	T11565	T11558	prep	in,removed
R7950	T11566	T11567	det	the,disease
R7951	T11567	T11565	pobj	disease,in
R7952	T11568	T11567	amod	human,disease
R7953	T11569	T11558	prep	than,removed
R7954	T11570	T11569	prep	in,than
R7955	T11571	T11572	det	the,models
R7956	T11572	T11570	pobj	models,in
R7957	T11573	T11572	compound	mouse,models
R7958	T11574	T11556	prep	through,is
R7959	T11575	T11576	amod	microglial,phagocytosis
R7960	T11576	T11574	pobj	phagocytosis,through
R7961	T11577	T11556	punct	.,is
R7962	T11579	T11580	amod	Resident,microglia
R7963	T11580	T11581	nsubj	microglia,localize
R7964	T11582	T11580	prep	in,microglia
R7965	T11583	T11584	amod	transgenic,models
R7966	T11584	T11582	pobj	models,in
R7967	T11585	T11584	compound	mouse,models
R7968	T11586	T11581	prep	to,localize
R7969	T11587	T11586	pobj	tissue,to
R7970	T11588	T11587	acl	surrounding,tissue
R7971	T11589	T11588	dobj	plaques,surrounding
R7972	T11590	T11581	cc	but,localize
R7973	T11591	T11581	conj	show,localize
R7974	T11592	T11593	amod	little,evidence
R7975	T11593	T11591	dobj	evidence,show
R7976	T11594	T11593	prep	of,evidence
R7977	T11595	T11596	compound	amyloid,engulfment
R7978	T11596	T11594	pobj	engulfment,of
R7979	T11597	T11598	punct	[,37
R7980	T11598	T11591	parataxis	37,show
R7981	T11599	T11600	punct	–,40
R7982	T11600	T11598	prep	40,37
R7983	T11601	T11598	punct	],37
R7984	T11602	T11581	punct	.,localize
R7985	T11604	T11605	prep	In,show
R7986	T11606	T11604	pobj	contrast,In
R7987	T11607	T11605	punct	", ",show
R7988	T11608	T11605	nsubj	microglia,show
R7989	T11609	T11608	acl	surrounding,microglia
R7990	T11610	T11611	compound	amyloid,plaques
R7991	T11611	T11609	dobj	plaques,surrounding
R7992	T11612	T11609	prep	in,surrounding
R7993	T11613	T11614	amod	human,brain
R7994	T11614	T11612	pobj	brain,in
R7995	T11615	T11616	det	a,state
R7996	T11616	T11605	dobj	state,show
R7997	T11617	T11618	advmod	much,higher
R7998	T11618	T11616	amod	higher,state
R7999	T11619	T11616	prep	of,state
R8000	T11620	T11619	pobj	activation,of
R8001	T11621	T11605	prep	with,show
R8002	T11622	T11623	amod	greater,expression
R8003	T11623	T11621	pobj	expression,with
R8004	T11624	T11623	prep	of,expression
R8005	T11625	T11626	compound	complement,receptor
R8006	T11626	T11624	pobj	receptor,of
R8007	T11627	T11628	punct	[,40
R8008	T11628	T11605	parataxis	40,show
R8009	T11629	T11628	punct	],40
R8010	T11630	T11605	punct	.,show
R8011	T11632	T11633	advmod	Thus,is
R8012	T11634	T11633	punct	", ",is
R8013	T11635	T11636	det	the,role
R8014	T11636	T11633	nsubj	role,is
R8015	T11637	T11636	prep	of,role
R8016	T11638	T11637	pobj	microglia,of
R8017	T11639	T11636	prep	in,role
R8018	T11640	T11641	compound	amyloid,metabolism
R8019	T11641	T11639	pobj	metabolism,in
R8020	T11642	T11633	acomp	minor,is
R8021	T11643	T11633	prep	in,is
R8022	T11644	T11645	amod	transgenic,models
R8023	T11645	T11643	pobj	models,in
R8024	T11646	T11633	prep	compared,is
R8025	T11647	T11646	prep	to,compared
R8026	T11648	T11649	det	the,condition
R8027	T11649	T11647	pobj	condition,to
R8028	T11650	T11649	amod	human,condition
R8029	T11651	T11633	punct	.,is
R8030	T11653	T11654	advmod	Somewhat,paradoxically
R8031	T11654	T11655	advmod	paradoxically,demonstrate
R8032	T11656	T11655	punct	", ",demonstrate
R8033	T11657	T11658	amod	several,studies
R8034	T11658	T11655	nsubj	studies,demonstrate
R8035	T11659	T11655	advmod	further,demonstrate
R8036	T11660	T11661	mark	that,lowers
R8037	T11661	T11655	ccomp	lowers,demonstrate
R8038	T11662	T11661	nsubj	treatment,lowers
R8039	T11663	T11662	prep	with,treatment
R8040	T11664	T11665	amod	anti-inflammatory,drugs
R8041	T11665	T11663	pobj	drugs,with
R8042	T11666	T11667	aux	to,reduce
R8043	T11667	T11662	advcl	reduce,treatment
R8044	T11668	T11669	amod	microglial,activation
R8045	T11669	T11667	dobj	activation,reduce
R8046	T11670	T11661	advmod	actually,lowers
R8047	T11671	T11672	compound	amyloid,load
R8048	T11672	T11661	dobj	load,lowers
R8049	T11673	T11661	prep	in,lowers
R8050	T11674	T11675	npadvmod	APP,transgenic
R8051	T11675	T11676	amod	transgenic,mice
R8052	T11676	T11673	pobj	mice,in
R8053	T11677	T11661	punct	", ",lowers
R8054	T11678	T11661	advcl	suggesting,lowers
R8055	T11679	T11680	det	a,role
R8056	T11680	T11678	dobj	role,suggesting
R8057	T11681	T11680	prep	for,role
R8058	T11682	T11683	compound	mouse,microglia
R8059	T11683	T11681	pobj	microglia,for
R8060	T11684	T11680	prep	in,role
R8061	T11685	T11686	det	the,formation
R8062	T11686	T11684	pobj	formation,in
R8063	T11687	T11686	cc	and,formation
R8064	T11688	T11686	conj	maintenance,formation
R8065	T11689	T11686	prep	of,formation
R8066	T11690	T11691	compound	amyloid,aggregates
R8067	T11691	T11689	pobj	aggregates,of
R8068	T11692	T11693	punct	[,41
R8069	T11693	T11661	parataxis	41,lowers
R8070	T11694	T11695	punct	–,43
R8071	T11695	T11693	prep	43,41
R8072	T11696	T11693	punct	],41
R8073	T11697	T11655	punct	.,demonstrate
R8074	T11699	T11700	advmod	However,explained
R8075	T11701	T11700	punct	", ",explained
R8076	T11702	T11703	det	this,outcome
R8077	T11703	T11700	nsubjpass	outcome,explained
R8078	T11704	T11700	aux	may,explained
R8079	T11705	T11700	auxpass	be,explained
R8080	T11706	T11700	advmod	alternatively,explained
R8081	T11707	T11700	agent	by,explained
R8082	T11708	T11709	amod	direct,effects
R8083	T11709	T11707	pobj	effects,by
R8084	T11710	T11709	prep	of,effects
R8085	T11711	T11712	amod	many,drugs
R8086	T11712	T11710	pobj	drugs,of
R8087	T11713	T11712	amod	anti-inflammatory,drugs
R8088	T11714	T11709	prep	on,effects
R8089	T11715	T11716	punct	γ,secretase
R8090	T11716	T11718	compound	secretase,cleavage
R8091	T11717	T11716	punct	-,secretase
R8092	T11718	T11714	pobj	cleavage,on
R8093	T11719	T11720	punct	[,44
R8094	T11720	T11700	parataxis	44,explained
R8095	T11721	T11722	punct	–,47
R8096	T11722	T11720	prep	47,44
R8097	T11723	T11720	punct	],44
R8098	T11724	T11700	punct	.,explained
R8099	T11726	T11727	advmod	Nonetheless,is
R8100	T11728	T11727	punct	", ",is
R8101	T11729	T11730	det	the,role
R8102	T11730	T11727	nsubj	role,is
R8103	T11731	T11730	prep	of,role
R8104	T11732	T11731	pobj	microglia,of
R8105	T11733	T11730	prep	in,role
R8106	T11734	T11735	preconj	both,condition
R8107	T11735	T11733	pobj	condition,in
R8108	T11736	T11735	det	the,condition
R8109	T11737	T11735	amod	human,condition
R8110	T11738	T11735	cc	and,condition
R8111	T11739	T11740	det	the,models
R8112	T11740	T11735	conj	models,condition
R8113	T11741	T11740	compound	mouse,models
R8114	T11742	T11743	advmod	poorly,understood
R8115	T11743	T11727	acomp	understood,is
R8116	T11744	T11727	punct	", ",is
R8117	T11745	T11727	cc	and,is
R8118	T11746	T11747	nsubj	differences,lead
R8119	T11747	T11727	conj	lead,is
R8120	T11748	T11746	prep	in,differences
R8121	T11749	T11750	amod	microglial,reactivity
R8122	T11750	T11748	pobj	reactivity,in
R8123	T11751	T11746	prep	between,differences
R8124	T11752	T11753	det	the,two
R8125	T11753	T11751	pobj	two,between
R8126	T11754	T11747	aux	could,lead
R8127	T11755	T11747	prep	to,lead
R8128	T11756	T11757	advmod	significantly,faster
R8129	T11757	T11758	amod	faster,clearance
R8130	T11758	T11755	pobj	clearance,to
R8131	T11759	T11758	compound	amyloid,clearance
R8132	T11760	T11758	prep	in,clearance
R8133	T11761	T11762	det	the,brains
R8134	T11762	T11760	pobj	brains,in
R8135	T11763	T11762	prep	of,brains
R8136	T11764	T11763	pobj	patients,of
R8137	T11765	T11764	prep	with,patients
R8138	T11766	T11765	pobj	AD,with
R8139	T11767	T11768	mark	than,observe
R8140	T11768	T11758	advcl	observe,clearance
R8141	T11769	T11768	nsubj	we,observe
R8142	T11770	T11768	prep	in,observe
R8143	T11771	T11772	det	the,mice
R8144	T11772	T11770	pobj	mice,in
R8145	T11773	T11774	compound	tet,off
R8146	T11774	T11772	compound	off,mice
R8147	T11775	T11774	punct	-,off
R8148	T11776	T11772	compound	APP,mice
R8149	T11777	T11747	punct	.,lead
R8150	T11779	T11780	prep	Given,think
R8151	T11781	T11782	det	the,role
R8152	T11782	T11779	pobj	role,Given
R8153	T11783	T11784	advmod	relatively,minor
R8154	T11784	T11782	amod	minor,role
R8155	T11785	T11782	acl	played,role
R8156	T11786	T11785	agent	by,played
R8157	T11787	T11786	pobj	microglia,by
R8158	T11788	T11785	prep	in,played
R8159	T11789	T11790	amod	other,models
R8160	T11790	T11788	pobj	models,in
R8161	T11791	T11790	compound	mouse,models
R8162	T11792	T11790	prep	of,models
R8163	T11793	T11792	pobj	amyloidosis,of
R8164	T11794	T11780	punct	", ",think
R8165	T11795	T11780	nsubj	we,think
R8166	T11796	T11797	nsubj	it,unlikely
R8167	T11797	T11780	advcl	unlikely,think
R8168	T11798	T11799	mark	that,influenced
R8169	T11799	T11797	advcl	influenced,unlikely
R8170	T11800	T11801	det	these,cells
R8171	T11801	T11799	nsubj	cells,influenced
R8172	T11802	T11799	aux	have,influenced
R8173	T11803	T11804	det	the,rate
R8174	T11804	T11799	dobj	rate,influenced
R8175	T11805	T11804	prep	of,rate
R8176	T11806	T11807	compound	amyloid,clearance
R8177	T11807	T11805	pobj	clearance,of
R8178	T11808	T11799	prep	in,influenced
R8179	T11809	T11810	det	the,mice
R8180	T11810	T11808	pobj	mice,in
R8181	T11811	T11812	compound	tet,off
R8182	T11812	T11810	compound	off,mice
R8183	T11813	T11812	punct	-,off
R8184	T11814	T11810	compound	APP,mice
R8185	T11815	T11780	punct	.,think
R8186	T11817	T11818	advmod	Even,so
R8187	T11818	T11819	advmod	so,considered
R8188	T11820	T11819	punct	", ",considered
R8189	T11821	T11819	nsubj	we,considered
R8190	T11822	T11823	det	the,possibility
R8191	T11823	T11819	dobj	possibility,considered
R8192	T11824	T11825	mark	that,altered
R8193	T11825	T11823	acl	altered,possibility
R8194	T11826	T11827	amod	chronic,treatment
R8195	T11827	T11825	nsubj	treatment,altered
R8196	T11828	T11827	compound	dox,treatment
R8197	T11829	T11825	aux	may,altered
R8198	T11830	T11825	aux	have,altered
R8199	T11831	T11832	det	the,state
R8200	T11832	T11825	dobj	state,altered
R8201	T11833	T11832	compound	activation,state
R8202	T11834	T11832	prep	of,state
R8203	T11835	T11834	pobj	microglia,of
R8204	T11836	T11825	prep	in,altered
R8205	T11837	T11838	poss	our,mice
R8206	T11838	T11836	pobj	mice,in
R8207	T11839	T11838	amod	treated,mice
R8208	T11840	T11819	punct	.,considered
R8209	T11842	T11843	nsubj	Dox,is
R8210	T11844	T11845	advmod	structurally,similar
R8211	T11845	T11843	acomp	similar,is
R8212	T11846	T11845	prep	to,similar
R8213	T11847	T11846	pobj	minocycline,to
R8214	T11848	T11847	punct	", ",minocycline
R8215	T11849	T11850	det	a,drug
R8216	T11850	T11847	appos	drug,minocycline
R8217	T11851	T11850	amod	reported,drug
R8218	T11852	T11850	amod	anti-inflammatory,drug
R8219	T11853	T11850	cc	and,drug
R8220	T11854	T11850	conj	inhibitor,drug
R8221	T11855	T11854	prep	of,inhibitor
R8222	T11856	T11857	amod	microglial,activation
R8223	T11857	T11855	pobj	activation,of
R8224	T11858	T11859	punct	[,48
R8225	T11859	T11843	parataxis	48,is
R8226	T11860	T11859	punct	],48
R8227	T11861	T11843	punct	.,is
R8228	T11863	T11864	advmod	However,expected
R8229	T11865	T11864	punct	", ",expected
R8230	T11866	T11867	mark	if,have
R8231	T11867	T11864	advcl	have,expected
R8232	T11868	T11867	nsubj	dox,have
R8233	T11869	T11867	aux	does,have
R8234	T11870	T11871	amod	anti-inflammatory,activity
R8235	T11871	T11867	dobj	activity,have
R8236	T11872	T11864	punct	", ",expected
R8237	T11873	T11864	advmod	then,expected
R8238	T11874	T11864	punct	", ",expected
R8239	T11875	T11864	prep	based,expected
R8240	T11876	T11875	prep	on,based
R8241	T11877	T11878	amod	previous,studies
R8242	T11878	T11876	pobj	studies,on
R8243	T11879	T11878	prep	with,studies
R8244	T11880	T11881	amod	other,anti-inflammatories
R8245	T11881	T11879	pobj	anti-inflammatories,with
R8246	T11882	T11864	punct	", ",expected
R8247	T11883	T11864	nsubj	we,expected
R8248	T11884	T11864	aux	would,expected
R8249	T11885	T11864	aux	have,expected
R8250	T11886	T11887	aux	to,find
R8251	T11887	T11864	xcomp	find,expected
R8252	T11888	T11889	advmod	less,amyloid
R8253	T11889	T11887	dobj	amyloid,find
R8254	T11890	T11887	prep	in,find
R8255	T11891	T11892	det	the,animals
R8256	T11892	T11890	pobj	animals,in
R8257	T11893	T11894	npadvmod	dox,treated
R8258	T11894	T11892	amod	treated,animals
R8259	T11895	T11894	punct	-,treated
R8260	T11896	T11864	punct	.,expected
R8261	T11898	T11899	advmod	Clearly,was
R8262	T11900	T11899	punct	", ",was
R8263	T11901	T11899	nsubj	that,was
R8264	T11902	T11899	neg	not,was
R8265	T11903	T11904	det	the,case
R8266	T11904	T11899	attr	case,was
R8267	T11905	T11899	punct	.,was
R8268	T11907	T11908	mark	While,is
R8269	T11908	T11910	advcl	is,demonstrate
R8270	T11909	T11908	nsubj	it,is
R8271	T11911	T11908	acomp	possible,is
R8272	T11912	T11913	mark	that,acts
R8273	T11913	T11908	ccomp	acts,is
R8274	T11914	T11913	nsubj	dox,acts
R8275	T11915	T11913	prep	in,acts
R8276	T11916	T11917	det	some,way
R8277	T11917	T11915	pobj	way,in
R8278	T11918	T11917	amod	other,way
R8279	T11919	T11920	aux	to,slow
R8280	T11920	T11913	advcl	slow,acts
R8281	T11921	T11922	compound	amyloid,clearance
R8282	T11922	T11920	dobj	clearance,slow
R8283	T11923	T11910	punct	", ",demonstrate
R8284	T11924	T11910	nsubj	data,demonstrate
R8285	T11925	T11924	prep	from,data
R8286	T11926	T11927	amod	multiple,studies
R8287	T11927	T11925	pobj	studies,from
R8288	T11928	T11929	mark	that,are
R8289	T11929	T11910	ccomp	are,demonstrate
R8290	T11930	T11931	amod	microglial,responses
R8291	T11931	T11929	nsubj	responses,are
R8292	T11932	T11929	advmod	normally,are
R8293	T11933	T11929	acomp	weak,are
R8294	T11934	T11929	prep	in,are
R8295	T11935	T11936	det	the,models
R8296	T11936	T11934	pobj	models,in
R8297	T11937	T11936	compound	mouse,models
R8298	T11938	T11936	compound	AD,models
R8299	T11939	T11940	punct	[,37
R8300	T11940	T11929	parataxis	37,are
R8301	T11941	T11942	punct	–,40
R8302	T11942	T11940	prep	40,37
R8303	T11943	T11940	punct	],37
R8304	T11944	T11929	punct	", ",are
R8305	T11945	T11929	cc	and,are
R8306	T11946	T11947	advmod	thus,is
R8307	T11947	T11929	conj	is,are
R8308	T11948	T11947	nsubj	it,is
R8309	T11949	T11947	acomp	doubtful,is
R8310	T11950	T11951	mark	that,affected
R8311	T11951	T11947	ccomp	affected,is
R8312	T11952	T11953	npadvmod	dox,mediated
R8313	T11953	T11955	amod	mediated,inhibition
R8314	T11954	T11953	punct	-,mediated
R8315	T11955	T11951	nsubj	inhibition,affected
R8316	T11956	T11955	amod	microglial,inhibition
R8317	T11957	T11958	det	the,outcome
R8318	T11958	T11951	dobj	outcome,affected
R8319	T11959	T11958	prep	of,outcome
R8320	T11960	T11961	poss	our,study
R8321	T11961	T11959	pobj	study,of
R8322	T11962	T11910	punct	.,demonstrate
R8323	T11964	T11965	det	The,persistence
R8324	T11965	T11966	nsubj	persistence,is
R8325	T11967	T11965	cc	and,persistence
R8326	T11968	T11965	conj	stability,persistence
R8327	T11969	T11965	prep	of,persistence
R8328	T11970	T11971	compound	amyloid,deposits
R8329	T11971	T11969	pobj	deposits,of
R8330	T11972	T11965	prep	in,persistence
R8331	T11973	T11974	poss	our,system
R8332	T11974	T11972	pobj	system,in
R8333	T11975	T11966	acomp	unexpected,is
R8334	T11976	T11966	prep	given,is
R8335	T11977	T11978	det	the,speed
R8336	T11978	T11976	pobj	speed,given
R8337	T11979	T11980	prep	with,cleared
R8338	T11980	T11978	relcl	cleared,speed
R8339	T11981	T11979	pobj	which,with
R8340	T11982	T11983	compound	Aβ,aggregates
R8341	T11983	T11980	nsubjpass	aggregates,cleared
R8342	T11984	T11980	auxpass	are,cleared
R8343	T11985	T11980	prep	in,cleared
R8344	T11986	T11987	amod	other,models
R8345	T11987	T11985	pobj	models,in
R8346	T11988	T11987	compound	mouse,models
R8347	T11989	T11987	prep	of,models
R8348	T11990	T11991	amod	therapeutic,intervention
R8349	T11991	T11989	pobj	intervention,of
R8350	T11992	T11966	punct	.,is
R8351	T11994	T11995	amod	Anti-Aβ,antibodies
R8352	T11995	T11996	nsubjpass	antibodies,shown
R8353	T11997	T11995	acl	injected,antibodies
R8354	T11998	T11997	advmod	directly,injected
R8355	T11999	T11997	prep	into,injected
R8356	T12000	T12001	det	the,brain
R8357	T12001	T11999	pobj	brain,into
R8358	T12002	T11996	aux	have,shown
R8359	T12003	T11996	auxpass	been,shown
R8360	T12004	T12005	aux	to,eliminate
R8361	T12005	T11996	xcomp	eliminate,shown
R8362	T12006	T12007	compound	amyloid,deposits
R8363	T12007	T12005	dobj	deposits,eliminate
R8364	T12008	T12005	prep	in,eliminate
R8365	T12009	T12010	advmod	as,1
R8366	T12010	T12013	nummod	1,wk
R8367	T12011	T12010	amod	little,1
R8368	T12012	T12010	quantmod	as,1
R8369	T12013	T12008	pobj	wk,in
R8370	T12014	T12013	prep	after,wk
R8371	T12015	T12014	pobj	treatment,after
R8372	T12016	T12017	punct	[,49
R8373	T12017	T12005	parataxis	49,eliminate
R8374	T12018	T12019	punct	–,51
R8375	T12019	T12017	prep	51,49
R8376	T12020	T12017	punct	],49
R8377	T12021	T11996	punct	.,shown
R8378	T12023	T12024	amod	Peripheral,injection
R8379	T12024	T12026	nsubj	injection,decreases
R8380	T12025	T12024	compound	antibody,injection
R8381	T12027	T12028	compound	amyloid,load
R8382	T12028	T12026	dobj	load,decreases
R8383	T12029	T12030	advmod	more,broadly
R8384	T12030	T12026	advmod	broadly,decreases
R8385	T12031	T12026	punct	", ",decreases
R8386	T12032	T12026	cc	and,decreases
R8387	T12033	T12034	mark	although,appear
R8388	T12034	T12038	advcl	appear,reduce
R8389	T12035	T12034	nsubj	it,appear
R8390	T12036	T12034	aux	does,appear
R8391	T12037	T12034	neg	not,appear
R8392	T12038	T12026	conj	reduce,decreases
R8393	T12039	T12040	aux	to,act
R8394	T12040	T12034	xcomp	act,appear
R8395	T12041	T12042	advmod	as,quickly
R8396	T12042	T12040	advmod	quickly,act
R8397	T12043	T12042	prep	as,quickly
R8398	T12044	T12045	amod	local,injection
R8399	T12045	T12043	pobj	injection,as
R8400	T12046	T12038	punct	", ",reduce
R8401	T12047	T12038	aux	can,reduce
R8402	T12048	T12038	advmod	significantly,reduce
R8403	T12086	T12084	pobj	area,in
R8404	T12049	T12050	compound	amyloid,load
R8405	T12087	T12086	prep	of,area
R8406	T12050	T12038	dobj	load,reduce
R8407	T12088	T12089	det	the,site
R8408	T12051	T12038	prep	within,reduce
R8409	T12052	T12053	nummod	2,mo
R8410	T12089	T12087	pobj	site,of
R8411	T12090	T12089	compound	injection,site
R8412	T12053	T12051	pobj	mo,within
R8413	T12091	T12092	punct	[,54
R8414	T12092	T12073	parataxis	54,reduce
R8415	T12054	T12053	prep	of,mo
R8416	T12093	T12092	punct	],54
R8417	T12094	T12066	punct	.,shown
R8418	T12055	T12056	amod	initial,treatment
R8419	T12096	T12097	amod	Careful,study
R8420	T12097	T12098	nsubj	study,suggested
R8421	T12056	T12054	pobj	treatment,of
R8422	T12099	T12097	prep	of,study
R8423	T12057	T12058	punct	[,53
R8424	T12100	T12101	det	the,mechanism
R8425	T12101	T12099	pobj	mechanism,of
R8426	T12058	T12038	parataxis	53,reduce
R8427	T12102	T12101	prep	behind,mechanism
R8428	T12103	T12102	pobj	several,behind
R8429	T12104	T12103	prep	of,several
R8430	T12059	T12058	nummod	52,53
R8431	T12105	T12106	det	the,therapies
R8432	T12106	T12104	pobj	therapies,of
R8433	T12107	T12108	npadvmod	antibody,mediated
R8434	T12108	T12106	amod	mediated,therapies
R8435	T12109	T12108	punct	-,mediated
R8436	T12060	T12058	punct	",",53
R8437	T12110	T12098	aux	has,suggested
R8438	T12111	T12112	mark	that,play
R8439	T12112	T12098	ccomp	play,suggested
R8440	T12061	T12058	punct	],53
R8441	T12113	T12114	amod	activated,microglia
R8442	T12114	T12112	nsubj	microglia,play
R8443	T12115	T12116	det	an,role
R8444	T12062	T12026	punct	.,decreases
R8445	T12116	T12112	dobj	role,play
R8446	T12117	T12116	amod	important,role
R8447	T12064	T12065	advmod	More,recently
R8448	T12118	T12112	prep	in,play
R8449	T12119	T12120	det	the,removal
R8450	T12120	T12118	pobj	removal,in
R8451	T12121	T12120	prep	of,removal
R8452	T12065	T12066	advmod	recently,shown
R8453	T12122	T12123	amod	fibrillar,plaques
R8454	T12123	T12121	pobj	plaques,of
R8455	T12124	T12112	prep	after,play
R8456	T12067	T12066	punct	", ",shown
R8457	T12125	T12124	pobj	immunization,after
R8458	T12126	T12127	punct	[,55
R8459	T12127	T12098	parataxis	55,suggested
R8461	T12068	T12069	det	an,approach
R8462	T12129	T12127	punct	",",55
R8463	T12130	T12127	nummod	52,55
R8464	T12131	T12127	punct	",",55
R8465	T12069	T12066	nsubj	approach,shown
R8466	T12132	T12127	punct	],55
R8467	T12133	T12098	punct	.,suggested
R8468	T12070	T12069	amod	alternative,approach
R8469	T12135	T12136	advmod	However,noted
R8470	T12071	T12066	aux	has,shown
R8471	T12137	T12136	punct	", ",noted
R8472	T12138	T12136	nsubjpass	it,noted
R8473	T12139	T12136	aux	has,noted
R8474	T12072	T12073	mark	that,reduce
R8475	T12140	T12136	auxpass	been,noted
R8476	T12141	T12142	mark	that,has
R8477	T12073	T12066	ccomp	reduce,shown
R8478	T12142	T12136	ccomp	has,noted
R8479	T12143	T12142	nsubj	deletion,has
R8480	T12144	T12143	prep	of,deletion
R8481	T12074	T12075	amod	lentiviral,transfer
R8482	T12145	T12146	det	the,receptor
R8483	T12146	T12144	pobj	receptor,of
R8484	T12147	T12146	compound	Fc,receptor
R8485	T12148	T12146	punct	(,receptor
R8486	T12149	T12150	det	the,receptor
R8487	T12075	T12073	nsubj	transfer,reduce
R8488	T12150	T12146	appos	receptor,receptor
R8489	T12151	T12150	amod	primary,receptor
R8490	T12152	T12150	prep	for,receptor
R8491	T12076	T12075	prep	of,transfer
R8492	T12153	T12154	amod	microglial,opsinization
R8493	T12154	T12152	pobj	opsinization,for
R8494	T12155	T12154	prep	of,opsinization
R8495	T12077	T12076	pobj	neprilysin,of
R8496	T12156	T12157	compound	antibody,antigen
R8497	T12157	T12159	compound	antigen,complexes
R8498	T12158	T12157	punct	–,antigen
R8499	T12078	T12073	aux	can,reduce
R8500	T12159	T12155	pobj	complexes,of
R8501	T12160	T12143	punct	),deletion
R8502	T12079	T12073	advmod	also,reduce
R8503	T12161	T12143	prep	in,deletion
R8504	T12162	T12163	npadvmod	APP,transgenic
R8505	T12163	T12164	amod	transgenic,models
R8506	T12080	T12081	det	the,number
R8507	T12164	T12161	pobj	models,in
R8508	T12165	T12164	compound	mouse,models
R8509	T12166	T12167	det	no,impact
R8510	T12081	T12073	dobj	number,reduce
R8511	T12167	T12142	dobj	impact,has
R8512	T12168	T12167	prep	on,impact
R8513	T12082	T12081	prep	of,number
R8514	T12169	T12170	det	the,effectiveness
R8515	T12170	T12168	pobj	effectiveness,on
R8516	T12171	T12170	prep	of,effectiveness
R8517	T12083	T12082	pobj	aggregates,of
R8518	T12172	T12173	npadvmod	antibody,mediated
R8519	T12084	T12073	prep	in,reduce
R8520	T12085	T12086	det	the,area
R8521	T12173	T12175	amod	mediated,therapy
R8522	T12192	T12191	prep	of,lack
R8523	T12174	T12173	punct	-,mediated
R8524	T12175	T12171	pobj	therapy,of
R8525	T12176	T12177	punct	[,57
R8526	T12177	T12142	parataxis	57,has
R8527	T12193	T12194	compound	microglia,activation
R8528	T12178	T12177	nummod	56,57
R8529	T12179	T12177	punct	",",57
R8530	T12180	T12177	punct	],57
R8531	T12194	T12192	pobj	activation,of
R8532	T12181	T12136	punct	.,noted
R8533	T12195	T12196	det	the,difference
R8534	T12183	T12184	nsubj	It,is
R8535	T12196	T12190	attr	difference,is
R8536	T12185	T12184	punct	", ",is
R8537	T12186	T12184	advmod	nevertheless,is
R8538	T12187	T12184	punct	", ",is
R8539	T12188	T12184	acomp	possible,is
R8540	T12189	T12190	mark	that,is
R8541	T12190	T12184	ccomp	is,is
R8542	T12197	T12196	amod	major,difference
R8543	T12191	T12190	nsubj	lack,is
R8544	T12198	T12196	prep	between,difference
R8545	T12199	T12200	det	the,clearance
R8546	T12200	T12198	pobj	clearance,between
R8547	T12201	T12200	amod	slow,clearance
R8548	T12202	T12200	acl	described,clearance
R8549	T12203	T12202	advmod	here,described
R8550	T12298	T12297	punct	],58
R8551	T12299	T12293	cc	or,expression
R8552	T12204	T12200	punct	", ",clearance
R8553	T12300	T12301	amod	central,injection
R8554	T12301	T12293	conj	injection,expression
R8555	T12302	T12301	prep	of,injection
R8556	T12205	T12206	advmod	where,expected
R8557	T12303	T12302	pobj	lipopolysaccharide,of
R8558	T12304	T12305	punct	[,60
R8559	T12305	T12303	parataxis	60,lipopolysaccharide
R8560	T12206	T12200	relcl	expected,clearance
R8561	T12306	T12305	nummod	59,60
R8562	T12307	T12305	punct	",",60
R8563	T12207	T12208	det	no,perturbation
R8564	T12308	T12305	punct	],60
R8565	T12309	T12282	aux	can,reduce
R8566	T12310	T12282	prep	by,reduce
R8567	T12208	T12206	nsubjpass	perturbation,expected
R8568	T12311	T12310	pobj	itself,by
R8569	T12312	T12282	advmod	substantially,reduce
R8570	T12209	T12208	prep	of,perturbation
R8571	T12313	T12314	compound	plaque,burden
R8572	T12314	T12282	dobj	burden,reduce
R8573	T12315	T12282	prep	in,reduce
R8574	T12210	T12211	det	the,system
R8575	T12316	T12317	npadvmod	APP,transgenic
R8576	T12317	T12318	amod	transgenic,mice
R8577	T12211	T12209	pobj	system,of
R8578	T12318	T12315	pobj	mice,in
R8579	T12319	T12282	punct	.,reduce
R8580	T12212	T12211	amod	immune,system
R8581	T12321	T12322	cc	But,reaggregates
R8582	T12213	T12206	auxpass	is,expected
R8583	T12323	T12322	prep	in,reaggregates
R8584	T12324	T12325	det	the,case
R8585	T12325	T12323	pobj	case,in
R8586	T12214	T12200	punct	", ",clearance
R8587	T12326	T12325	prep	of,case
R8588	T12327	T12328	amod	acute,activation
R8589	T12328	T12326	pobj	activation,of
R8590	T12329	T12330	npadvmod	antibody,mediated
R8591	T12330	T12328	amod	mediated,activation
R8592	T12215	T12200	cc	and,clearance
R8593	T12331	T12329	punct	-,antibody
R8594	T12332	T12329	cc	and,antibody
R8595	T12333	T12332	punct	/,and
R8596	T12216	T12217	det	the,clearance
R8597	T12334	T12332	cc	or,and
R8598	T12335	T12329	conj	injury,antibody
R8599	T12336	T12330	punct	-,mediated
R8600	T12217	T12200	conj	clearance,clearance
R8601	T12337	T12322	punct	", ",reaggregates
R8602	T12338	T12339	mark	once,passed
R8603	T12339	T12322	advcl	passed,reaggregates
R8604	T12218	T12217	amod	rapid,clearance
R8605	T12340	T12341	det	the,inflammation
R8606	T12341	T12339	nsubj	inflammation,passed
R8607	T12219	T12217	acl	described,clearance
R8608	T12342	T12339	aux	has,passed
R8609	T12220	T12219	prep	in,described
R8610	T12343	T12339	punct	", ",passed
R8611	T12221	T12220	pobj	studies,in
R8612	T12344	T12339	cc	and,passed
R8613	T12345	T12346	det	the,antibody
R8614	T12346	T12347	nsubjpass	antibody,cleared
R8615	T12222	T12221	acl	involving,studies
R8616	T12347	T12339	conj	cleared,passed
R8617	T12223	T12224	nmod	antibody,injection
R8618	T12348	T12346	cc	and,antibody
R8619	T12349	T12350	amod	bound,peptide
R8620	T12350	T12346	conj	peptide,antibody
R8621	T12224	T12222	dobj	injection,involving
R8622	T12351	T12347	aux	have,cleared
R8623	T12352	T12347	auxpass	been,cleared
R8624	T12353	T12347	cc	and,cleared
R8625	T12225	T12223	cc	or,antibody
R8626	T12354	T12347	conj	degraded,cleared
R8627	T12355	T12322	punct	", ",reaggregates
R8628	T12356	T12357	det	the,Aβ
R8629	T12226	T12223	conj	viral,antibody
R8630	T12357	T12322	nsubj	Aβ,reaggregates
R8631	T12358	T12357	amod	remaining,Aβ
R8632	T12227	T12184	punct	.,is
R8633	T12359	T12322	advmod	quickly,reaggregates
R8634	T12360	T12322	cc	and,reaggregates
R8635	T12361	T12362	compound	amyloid,pathology
R8636	T12229	T12230	prep	In,be
R8637	T12362	T12363	nsubjpass	pathology,reestablished
R8638	T12363	T12322	conj	reestablished,reaggregates
R8639	T12364	T12363	auxpass	is,reestablished
R8640	T12365	T12366	punct	[,49
R8641	T12366	T12363	parataxis	49,reestablished
R8642	T12367	T12366	punct	],49
R8643	T12368	T12322	punct	.,reaggregates
R8644	T12231	T12229	pobj	isolation,In
R8645	T12370	T12371	det	This,finding
R8646	T12371	T12372	nsubj	finding,reinforces
R8647	T12232	T12230	punct	", ",be
R8648	T12373	T12374	det	the,notion
R8649	T12374	T12372	dobj	notion,reinforces
R8650	T12375	T12376	mark	that,maintain
R8651	T12233	T12234	amod	mild,activation
R8652	T12376	T12374	acl	maintain,notion
R8653	T12377	T12376	prep	without,maintain
R8654	T12234	T12230	nsubj	activation,be
R8655	T12378	T12379	amod	continued,stimulation
R8656	T12379	T12377	pobj	stimulation,without
R8657	T12380	T12376	punct	", ",maintain
R8658	T12235	T12234	prep	of,activation
R8659	T12381	T12376	nsubj	microglia,maintain
R8660	T12382	T12381	prep	in,microglia
R8661	T12236	T12235	pobj	microglia,of
R8662	T12383	T12384	compound	mouse,models
R8663	T12384	T12382	pobj	models,in
R8664	T12385	T12376	aux	do,maintain
R8665	T12237	T12234	prep	by,activation
R8666	T12386	T12376	neg	not,maintain
R8667	T12387	T12388	det	the,level
R8668	T12388	T12376	dobj	level,maintain
R8669	T12238	T12239	compound	injection,damage
R8670	T12389	T12388	amod	same,level
R8671	T12390	T12388	prep	of,level
R8672	T12239	T12237	pobj	damage,by
R8673	T12391	T12392	amod	sustained,activation
R8674	T12392	T12390	pobj	activation,of
R8675	T12393	T12394	dep	that,occur
R8676	T12240	T12239	cc	or,damage
R8677	T12394	T12388	relcl	occur,level
R8678	T12395	T12394	aux	may,occur
R8679	T12241	T12239	conj	opsinization,damage
R8680	T12396	T12394	prep	in,occur
R8681	T12397	T12396	pobj	humans,in
R8682	T12398	T12372	punct	.,reinforces
R8683	T12242	T12230	aux	may,be
R8684	T12400	T12401	nsubj	SantaCruz,published
R8685	T12243	T12230	neg	not,be
R8686	T12402	T12403	advmod	et,al.
R8687	T12403	T12400	advmod	al.,SantaCruz
R8688	T12244	T12230	acomp	adequate,be
R8689	T12245	T12246	aux	to,induce
R8690	T12246	T12230	advcl	induce,be
R8691	T12404	T12401	advmod	recently,published
R8692	T12247	T12248	amod	substantial,phagocytosis
R8693	T12405	T12406	det	a,study
R8694	T12406	T12401	dobj	study,published
R8695	T12407	T12406	prep	of,study
R8696	T12408	T12407	pobj	mice,of
R8697	T12409	T12410	dep	that,express
R8698	T12410	T12408	relcl	express,mice
R8699	T12248	T12246	dobj	phagocytosis,induce
R8700	T12411	T12412	nmod	P301L,tau
R8701	T12412	T12410	dobj	tau,express
R8702	T12249	T12230	punct	", ",be
R8703	T12413	T12412	amod	human,tau
R8704	T12414	T12410	prep	via,express
R8705	T12415	T12416	det	a,system
R8706	T12250	T12230	cc	but,be
R8707	T12416	T12414	pobj	system,via
R8708	T12417	T12416	amod	similar,system
R8709	T12251	T12252	advmod	when,combined
R8710	T12418	T12416	compound	vector,system
R8711	T12419	T12420	punct	[,61
R8712	T12420	T12401	parataxis	61,published
R8713	T12252	T12253	advcl	combined,work
R8714	T12421	T12420	punct	],61
R8715	T12422	T12401	punct	.,published
R8716	T12253	T12230	conj	work,be
R8717	T12424	T12425	prep	As,found
R8718	T12254	T12252	prep	with,combined
R8719	T12426	T12424	prep	in,As
R8720	T12427	T12428	poss	our,mice
R8721	T12255	T12256	det	an,agent
R8722	T12256	T12254	pobj	agent,with
R8723	T12428	T12426	pobj	mice,in
R8724	T12429	T12430	compound	tet,off
R8725	T12257	T12258	npadvmod	Aβ,lowering
R8726	T12430	T12428	compound	off,mice
R8727	T12431	T12430	punct	-,off
R8728	T12258	T12256	amod	lowering,agent
R8729	T12432	T12428	compound	APP,mice
R8730	T12433	T12425	punct	", ",found
R8731	T12434	T12425	nsubj	SantaCruz,found
R8732	T12259	T12258	punct	-,lowering
R8733	T12435	T12436	advmod	et,al.
R8734	T12436	T12434	advmod	al.,SantaCruz
R8735	T12437	T12438	mark	that,cleared
R8736	T12260	T12256	punct	", ",agent
R8737	T12438	T12425	ccomp	cleared,found
R8738	T12439	T12440	nmod	tau,tangles
R8739	T12261	T12262	amod	such,as
R8740	T12440	T12438	nsubjpass	tangles,cleared
R8741	T12441	T12440	amod	neurofibrillary,tangles
R8742	T12262	T12256	prep	as,agent
R8743	T12442	T12440	punct	", ",tangles
R8744	T12443	T12440	prep	like,tangles
R8745	T12444	T12445	compound	amyloid,plaques
R8746	T12445	T12443	pobj	plaques,like
R8747	T12446	T12438	punct	", ",cleared
R8748	T12263	T12264	npadvmod	neprilysin,targeted
R8749	T12447	T12438	auxpass	are,cleared
R8750	T12448	T12438	neg	not,cleared
R8751	T12449	T12438	advmod	efficiently,cleared
R8752	T12450	T12438	prep	following,cleared
R8753	T12264	T12268	amod	targeted,antibodies
R8754	T12451	T12452	compound	transgene,suppression
R8755	T12452	T12450	pobj	suppression,following
R8756	T12453	T12425	punct	.,found
R8757	T12265	T12263	cc	or,neprilysin
R8758	T12455	T12456	det	The,lack
R8759	T12456	T12457	nsubj	lack,comes
R8760	T12266	T12263	conj	Aβ,neprilysin
R8761	T12458	T12456	prep	of,lack
R8762	T12267	T12264	punct	-,targeted
R8763	T12459	T12458	pobj	clearance,of
R8764	T12460	T12456	prep	in,lack
R8765	T12461	T12462	det	both,models
R8766	T12268	T12262	pobj	antibodies,as
R8767	T12462	T12460	pobj	models,in
R8768	T12463	T12462	prep	of,models
R8769	T12269	T12253	punct	", ",work
R8770	T12464	T12465	compound	AD,pathology
R8771	T12465	T12463	pobj	pathology,of
R8772	T12466	T12457	prep	as,comes
R8773	T12270	T12271	det	the,two
R8774	T12467	T12468	det	a,contrast
R8775	T12468	T12466	pobj	contrast,as
R8776	T12271	T12253	nsubj	two,work
R8777	T12469	T12468	amod	stark,contrast
R8778	T12470	T12468	prep	to,contrast
R8779	T12471	T12472	det	the,removal
R8780	T12272	T12253	aux	may,work
R8781	T12472	T12470	pobj	removal,to
R8782	T12473	T12472	amod	rapid,removal
R8783	T12273	T12253	prep	in,work
R8784	T12474	T12472	prep	of,removal
R8785	T12475	T12476	compound	protein,aggregates
R8786	T12476	T12474	pobj	aggregates,of
R8787	T12274	T12273	pobj	concert,in
R8788	T12477	T12472	acl	found,removal
R8789	T12478	T12477	prep	in,found
R8790	T12479	T12480	amod	similar,models
R8791	T12275	T12276	aux	to,clear
R8792	T12480	T12478	pobj	models,in
R8793	T12481	T12482	compound	tet,off
R8794	T12276	T12253	advcl	clear,work
R8795	T12482	T12480	compound	off,models
R8797	T12484	T12480	compound	mouse,models
R8798	T12485	T12480	prep	of,models
R8799	T12486	T12487	nmod	Huntington,disease
R8800	T12487	T12485	pobj	disease,of
R8801	T12277	T12278	compound	peptide,deposits
R8805	T12278	T12276	dobj	deposits,clear
R8806	T12491	T12486	cc	and,Huntington
R8807	T12492	T12486	conj	prion,Huntington
R8809	T12279	T12253	punct	.,work
R8810	T12494	T12487	parataxis	62,disease
R8811	T12495	T12494	punct	],62
R8812	T12281	T12282	advcl	Consistent,reduce
R8813	T12496	T12457	punct	.,comes
R8814	T12498	T12499	prep	In,led
R8815	T12283	T12281	prep	with,Consistent
R8816	T12500	T12501	det	these,cases
R8817	T12501	T12498	pobj	cases,In
R8818	T12502	T12499	punct	", ",led
R8819	T12503	T12499	csubj	disrupting,led
R8820	T12284	T12285	det	this,hypothesis
R8821	T12504	T12505	det	the,input
R8822	T12505	T12503	dobj	input,disrupting
R8823	T12506	T12505	prep	of,input
R8824	T12285	T12283	pobj	hypothesis,with
R8825	T12507	T12508	amod	new,monomer
R8826	T12508	T12506	pobj	monomer,of
R8827	T12509	T12505	prep	to,input
R8828	T12286	T12282	punct	", ",reduce
R8829	T12287	T12288	amod	strong,activation
R8830	T12288	T12282	nsubj	activation,reduce
R8831	T12289	T12288	prep	of,activation
R8832	T12510	T12511	det	the,system
R8833	T12290	T12289	pobj	microglia,of
R8834	T12511	T12509	pobj	system,to
R8835	T12512	T12503	prep	via,disrupting
R8836	T12513	T12514	npadvmod	dox,mediated
R8837	T12291	T12288	prep	through,activation
R8838	T12292	T12293	amod	transgenic,expression
R8839	T12514	T12516	amod	mediated,suppression
R8840	T12293	T12291	pobj	expression,through
R8841	T12515	T12514	punct	-,mediated
R8842	T12516	T12512	pobj	suppression,via
R8843	T12517	T12516	compound	transgene,suppression
R8844	T12294	T12293	prep	of,expression
R8845	T12518	T12499	prep	to,led
R8846	T12519	T12520	advmod	relatively,rapid
R8847	T12295	T12294	pobj	TGFβ,of
R8848	T12520	T12521	amod	rapid,clearance
R8849	T12521	T12518	pobj	clearance,to
R8850	T12522	T12521	prep	of,clearance
R8851	T12523	T12524	compound	protein,aggregates
R8852	T12296	T12297	punct	[,58
R8853	T12524	T12522	pobj	aggregates,of
R8854	T12525	T12499	punct	.,led
R8855	T12297	T12295	parataxis	58,TGFβ
R8856	T12527	T12528	prep	By,suggest
R8857	T12529	T12527	pobj	contrast,By
R8858	T12616	T12615	punct	-,off
R8859	T12530	T12528	punct	", ",suggest
R8860	T12531	T12532	poss	our,study
R8861	T12532	T12528	nsubj	study,suggest
R8862	T12617	T12613	compound	tau,mice
R8863	T12533	T12532	cc	and,study
R8864	T12534	T12532	conj	that,study
R8865	T12535	T12534	prep	of,that
R8866	T12618	T12619	mark	that,required
R8867	T12536	T12535	pobj	SantaCruz,of
R8868	T12537	T12538	advmod	et,al.
R8869	T12538	T12536	advmod	al.,SantaCruz
R8870	T12619	T12610	ccomp	required,suggest
R8871	T12539	T12540	mark	that,be
R8872	T12540	T12528	ccomp	be,suggest
R8873	T12541	T12542	compound	protein,aggregates
R8874	T12620	T12621	compound	protein,clearance
R8875	T12542	T12540	nsubj	aggregates,be
R8876	T12543	T12542	prep	in,aggregates
R8877	T12621	T12619	nsubjpass	clearance,required
R8878	T12544	T12543	pobj	AD,in
R8879	T12545	T12540	aux	may,be
R8880	T12546	T12547	advmod	more,tenacious
R8881	T12622	T12619	aux	may,required
R8882	T12547	T12540	acomp	tenacious,be
R8883	T12548	T12547	prep	than,tenacious
R8884	T12549	T12548	prep	in,than
R8885	T12623	T12619	prep	in,required
R8886	T12550	T12551	amod	other,disorders
R8887	T12551	T12549	pobj	disorders,in
R8888	T12624	T12623	pobj	fact,in
R8889	T12552	T12551	amod	neurodegenerative,disorders
R8890	T12553	T12528	punct	.,suggest
R8891	T12625	T12619	neg	not,required
R8892	T12555	T12556	advmod	Perhaps,are
R8893	T12626	T12619	auxpass	be,required
R8894	T12557	T12558	mark	once,aggregated
R8895	T12558	T12556	advcl	aggregated,are
R8896	T12627	T12619	prep	for,required
R8897	T12559	T12556	punct	", ",are
R8898	T12560	T12556	nsubj	Aβ,are
R8899	T12561	T12560	cc	and,Aβ
R8900	T12628	T12629	amod	cognitive,improvement
R8901	T12562	T12560	conj	tau,Aβ
R8902	T12563	T12564	advmod	either,stable
R8903	T12564	T12556	acomp	stable,are
R8904	T12565	T12564	advmod	inherently,stable
R8905	T12566	T12564	advmod	more,stable
R8906	T12629	T12627	pobj	improvement,for
R8907	T12567	T12564	prep	than,stable
R8908	T12568	T12569	amod	other,aggregates
R8909	T12569	T12567	pobj	aggregates,than
R8910	T12630	T12619	prep	following,required
R8911	T12570	T12569	compound	protein,aggregates
R8912	T12571	T12564	cc	or,stable
R8913	T12572	T12573	advmod	more,resistant
R8914	T12631	T12632	compound	transgene,suppression
R8915	T12573	T12564	conj	resistant,stable
R8916	T12574	T12573	prep	to,resistant
R8917	T12575	T12576	advmod	intra,extracellular
R8918	T12632	T12630	pobj	suppression,following
R8919	T12576	T12579	amod	extracellular,mechanisms
R8920	T12577	T12576	punct	-,extracellular
R8921	T12633	T12634	punct	[,61
R8922	T12578	T12576	cc	and,extracellular
R8923	T12579	T12574	pobj	mechanisms,to
R8924	T12580	T12579	compound	clearance,mechanisms
R8925	T12634	T12619	parataxis	61,required
R8926	T12581	T12556	punct	.,are
R8927	T12635	T12634	punct	],61
R8928	T12583	T12584	nummod	One,question
R8929	T12584	T12585	nsubj	question,is
R8930	T12636	T12610	punct	.,suggest
R8931	T12586	T12587	nsubj	we,were
R8932	T12587	T12584	advcl	were,question
R8933	T12638	T12639	prep	At,is
R8934	T12588	T12587	neg	not,were
R8935	T12589	T12587	acomp	able,were
R8936	T12590	T12591	aux	to,address
R8937	T12591	T12589	xcomp	address,able
R8938	T12640	T12638	amod	present,At
R8939	T12592	T12591	prep	in,address
R8940	T12593	T12594	det	this,study
R8941	T12594	T12592	pobj	study,in
R8942	T12595	T12596	mark	whether,halts
R8943	T12641	T12639	punct	", ",is
R8944	T12596	T12585	ccomp	halts,is
R8945	T12597	T12596	csubj	abrogating,halts
R8946	T12642	T12639	prep	because,is
R8947	T12598	T12597	dobj	synthesis,abrogating
R8948	T12599	T12598	prep	of,synthesis
R8949	T12643	T12642	pcomp	of,because
R8950	T12600	T12601	amod	new,Aβ
R8951	T12601	T12599	pobj	Aβ,of
R8952	T12644	T12645	amod	unexpected,abnormalities
R8953	T12602	T12603	det	the,progression
R8954	T12603	T12596	dobj	progression,halts
R8955	T12604	T12603	prep	of,progression
R8956	T12645	T12642	pobj	abnormalities,because
R8957	T12605	T12606	amod	cognitive,decline
R8958	T12606	T12604	pobj	decline,of
R8959	T12607	T12585	punct	.,is
R8960	T12646	T12645	amod	noncognitive,abnormalities
R8961	T12609	T12610	nsubj	Studies,suggest
R8962	T12647	T12645	amod	behavioral,abnormalities
R8963	T12611	T12609	prep	from,Studies
R8964	T12612	T12613	det	the,mice
R8965	T12648	T12639	punct	", ",is
R8966	T12613	T12611	pobj	mice,from
R8967	T12614	T12615	compound	tet,off
R8968	T12649	T12639	nsubj	it,is
R8969	T12615	T12613	compound	off,mice
R8970	T12650	T12639	neg	not,is
R8971	T12651	T12639	acomp	clear,is
R8972	T12652	T12653	mark	whether,used
R8973	T12653	T12639	ccomp	used,is
R8974	T12722	T12723	advmod	when,tested
R8975	T12654	T12655	det	the,mice
R8976	T12723	T12705	advcl	tested,showed
R8977	T12724	T12723	prep	in,tested
R8978	T12655	T12653	nsubjpass	mice,used
R8979	T12725	T12726	det	the,maze
R8980	T12726	T12724	pobj	maze,in
R8981	T12656	T12657	compound	tet,off
R8982	T12727	T12726	compound	Morris,maze
R8983	T12728	T12726	compound	water,maze
R8984	T12729	T12705	punct	.,showed
R8985	T12657	T12655	compound	off,mice
R8986	T12731	T12732	nsubj	Expression,makes
R8987	T12658	T12657	punct	-,off
R8988	T12733	T12731	prep	of,Expression
R8989	T12734	T12735	det	this,phenotype
R8990	T12659	T12655	compound	APP,mice
R8991	T12735	T12733	pobj	phenotype,of
R8992	T12736	T12737	amod	standard,tests
R8993	T12737	T12738	nsubj	tests,uninterpretable
R8994	T12660	T12653	aux	can,used
R8995	T12738	T12732	ccomp	uninterpretable,makes
R8996	T12739	T12737	prep	of,tests
R8997	T12740	T12739	pobj	learning,of
R8998	T12661	T12653	auxpass	be,used
R8999	T12741	T12740	cc	and,learning
R9000	T12742	T12740	conj	memory,learning
R9001	T12662	T12663	aux	to,address
R9002	T12743	T12732	punct	.,makes
R9003	T12745	T12746	nsubj	Hyperactivity,inhibits
R9004	T12663	T12653	advcl	address,used
R9005	T12747	T12746	advmod	nonspecifically,inhibits
R9006	T12748	T12749	npadvmod	choice,driven
R9007	T12749	T12751	amod	driven,changes
R9008	T12664	T12665	det	the,question
R9009	T12750	T12749	punct	-,driven
R9010	T12751	T12746	dobj	changes,inhibits
R9011	T12665	T12663	dobj	question,address
R9012	T12752	T12751	prep	in,changes
R9013	T12753	T12752	pobj	movement,in
R9014	T12666	T12665	amod	same,question
R9015	T12754	T12751	punct	", ",changes
R9016	T12755	T12756	det	the,element
R9017	T12756	T12751	appos	element,changes
R9018	T12667	T12663	prep	in,address
R9019	T12757	T12756	amod	key,element
R9020	T12758	T12756	prep	behind,element
R9021	T12759	T12760	det	all,paradigms
R9022	T12668	T12669	det	the,context
R9023	T12760	T12758	pobj	paradigms,behind
R9024	T12761	T12760	amod	cognitive,paradigms
R9025	T12669	T12667	pobj	context,in
R9026	T12762	T12760	amod	behavioral,paradigms
R9027	T12763	T12746	punct	.,inhibits
R9028	T12670	T12669	prep	of,context
R9029	T12765	T12766	nsubj	We,working
R9030	T12671	T12672	compound	amyloid,pathology
R9031	T12767	T12766	aux	are,working
R9032	T12768	T12766	advmod	currently,working
R9033	T12769	T12770	aux	to,determine
R9034	T12672	T12670	pobj	pathology,of
R9035	T12770	T12766	xcomp	determine,working
R9036	T12771	T12772	mark	whether,correlates
R9037	T12772	T12770	ccomp	correlates,determine
R9038	T12673	T12639	punct	.,is
R9039	T12773	T12772	nsubj	hyperactivity,correlates
R9040	T12774	T12772	prep	with,correlates
R9041	T12675	T12676	det	Both,lines
R9042	T12775	T12774	pobj	expression,with
R9043	T12776	T12775	prep	of,expression
R9044	T12777	T12778	det	the,holoprotein
R9045	T12676	T12677	nsubj	lines,display
R9046	T12678	T12676	prep	of,lines
R9047	T12778	T12776	pobj	holoprotein,of
R9048	T12779	T12780	compound	APPswe,ind
R9049	T12780	T12778	compound	ind,holoprotein
R9050	T12781	T12780	punct	/,ind
R9051	T12679	T12680	compound	tTA,APP
R9052	T12782	T12775	cc	or,expression
R9053	T12783	T12784	poss	its,derivatives
R9054	T12784	T12775	conj	derivatives,expression
R9055	T12680	T12682	compound	APP,mice
R9056	T12785	T12784	amod	proteolytic,derivatives
R9057	T12786	T12766	punct	.,working
R9058	T12788	T12789	amod	Preliminary,studies
R9059	T12681	T12680	punct	/,APP
R9060	T12789	T12790	nsubj	studies,suggest
R9061	T12791	T12792	mark	that,appear
R9062	T12682	T12678	pobj	mice,of
R9063	T12792	T12790	ccomp	appear,suggest
R9064	T12793	T12792	nsubj	hyperactivity,appear
R9065	T12683	T12684	nsubj	we,studied
R9066	T12794	T12792	aux	does,appear
R9067	T12795	T12792	neg	not,appear
R9068	T12684	T12676	advcl	studied,lines
R9069	T12796	T12792	advmod	quickly,appear
R9070	T12797	T12798	advmod	when,shifted
R9071	T12798	T12792	advcl	shifted,appear
R9072	T12685	T12684	advmod	here,studied
R9073	T12799	T12800	npadvmod	dox,reared
R9074	T12800	T12802	amod	reared,mice
R9075	T12801	T12800	punct	-,reared
R9076	T12686	T12687	amod	extreme,hyperactivity
R9077	T12802	T12798	nsubjpass	mice,shifted
R9078	T12803	T12798	auxpass	are,shifted
R9079	T12687	T12677	dobj	hyperactivity,display
R9080	T12804	T12798	prep	to,shifted
R9081	T12805	T12806	amod	nonmedicated,diets
R9082	T12806	T12804	pobj	diets,to
R9083	T12688	T12687	amod	visible,hyperactivity
R9084	T12807	T12808	punct	(,data
R9085	T12808	T12798	parataxis	data,shifted
R9086	T12809	T12810	compound	J.,J.
R9087	T12689	T12688	prep	as,visible
R9088	T12810	T12808	dep	J.,data
R9089	T12811	T12810	compound	L.,J.
R9090	T12812	T12808	punct	", ",data
R9091	T12690	T12691	compound	cage,circling
R9092	T12813	T12808	amod	unpublished,data
R9093	T12814	T12808	punct	),data
R9094	T12815	T12790	punct	.,suggest
R9095	T12691	T12689	pobj	circling,as
R9096	T12817	T12818	det	These,data
R9097	T12692	T12688	cc	and,visible
R9098	T12818	T12819	nsubj	data,indicate
R9099	T12820	T12819	aux	may,indicate
R9100	T12693	T12688	conj	quantified,visible
R9101	T12821	T12822	mark	that,is
R9102	T12822	T12819	ccomp	is,indicate
R9103	T12694	T12693	agent	by,quantified
R9104	T12823	T12824	det	the,culprit
R9105	T12824	T12822	nsubj	culprit,is
R9106	T12825	T12824	amod	neuroactive,culprit
R9107	T12695	T12696	compound	activity,monitoring
R9108	T12826	T12827	neg	not,immediately
R9109	T12827	T12822	advmod	immediately,is
R9110	T12696	T12694	pobj	monitoring,by
R9111	T12697	T12698	punct	(,see
R9112	T12828	T12822	acomp	present,is
R9113	T12698	T12677	parataxis	see,display
R9114	T12829	T12830	mark	after,initiated
R9115	T12830	T12822	advcl	initiated,is
R9116	T12831	T12832	amod	transgenic,synthesis
R9117	T12699	T12698	dobj	Figure,see
R9118	T12832	T12830	nsubjpass	synthesis,initiated
R9119	T12700	T12699	nummod	7,Figure
R9121	T12834	T12830	auxpass	is,initiated
R9122	T12835	T12822	punct	", ",is
R9123	T12701	T12698	punct	),see
R9124	T12836	T12822	cc	but,is
R9125	T12837	T12822	conj	requires,is
R9126	T12702	T12677	punct	.,display
R9129	T12704	T12705	nsubj	Many,showed
R9130	T12840	T12841	aux	to,develop
R9131	T12841	T12839	advcl	develop,time
R9132	T12842	T12819	punct	.,indicate
R9133	T12706	T12704	prep	of,Many
R9134	T12844	T12845	advmod	Alternatively,caused
R9135	T12707	T12708	det	the,mice
R9136	T12846	T12845	punct	", ",caused
R9137	T12847	T12845	nsubjpass	hyperactivity,caused
R9138	T12708	T12706	pobj	mice,of
R9139	T12848	T12845	aux	may,caused
R9140	T12849	T12845	auxpass	be,caused
R9141	T12850	T12845	agent	by,caused
R9142	T12709	T12710	amod	double,transgenic
R9143	T12851	T12852	amod	neuronal,alterations
R9144	T12852	T12850	pobj	alterations,by
R9145	T12853	T12845	prep	due,caused
R9146	T12710	T12708	amod	transgenic,mice
R9147	T12854	T12853	pcomp	to,due
R9148	T12855	T12856	compound	transgene,expression
R9149	T12711	T12712	amod	similar,patterns
R9150	T12856	T12853	pobj	expression,due
R9151	T12857	T12856	prep	during,expression
R9152	T12858	T12859	amod	early,postnatal
R9153	T12712	T12705	dobj	patterns,showed
R9154	T12859	T12860	amod	postnatal,development
R9155	T12860	T12857	pobj	development,during
R9156	T12861	T12845	punct	.,caused
R9157	T12713	T12712	amod	circular,patterns
R9158	T12863	T12864	amod	Further,experiments
R9159	T12714	T12712	prep	of,patterns
R9160	T12864	T12865	nsubjpass	experiments,needed
R9161	T12866	T12865	auxpass	are,needed
R9162	T12715	T12714	pobj	swimming,of
R9163	T12867	T12868	aux	to,distinguish
R9164	T12868	T12865	advcl	distinguish,needed
R9165	T12869	T12868	prep	between,distinguish
R9166	T12716	T12712	prep	near,patterns
R9167	T12870	T12871	det	these,possibilities
R9168	T12871	T12869	pobj	possibilities,between
R9169	T12717	T12718	det	the,edge
R9170	T12872	T12865	punct	.,needed
R9171	T12874	T12875	prep	In,demonstrate
R9172	T12718	T12716	pobj	edge,near
R9173	T12876	T12874	pobj	summary,In
R9174	T12719	T12718	prep	of,edge
R9175	T12877	T12875	punct	", ",demonstrate
R9176	T12878	T12875	nsubj	we,demonstrate
R9177	T12720	T12721	det	the,tank
R9178	T12879	T12880	mark	that,halts
R9179	T12880	T12875	ccomp	halts,demonstrate
R9180	T12721	T12719	pobj	tank,of
R9181	T12881	T12880	csubj	abrogating,halts
R9182	T12882	T12883	compound	Aβ,production
R9183	T12883	T12881	dobj	production,abrogating
R9184	T12934	T12932	advcl	limit,therapies
R9185	T12884	T12885	det	the,progression
R9186	T12885	T12880	dobj	progression,halts
R9187	T12886	T12885	prep	of,progression
R9188	T12887	T12888	amod	pathologic,changes
R9189	T12935	T12936	compound	Aβ,production
R9190	T12888	T12886	pobj	changes,of
R9191	T12889	T12885	prep	in,progression
R9192	T12890	T12891	det	a,model
R9193	T12936	T12934	dobj	production,limit
R9194	T12891	T12889	pobj	model,in
R9195	T12892	T12891	amod	transgenic,model
R9196	T12937	T12934	punct	", ",limit
R9197	T12893	T12891	compound	mouse,model
R9198	T12894	T12891	prep	of,model
R9199	T12895	T12896	compound	Alzheimer,type
R9200	T12938	T12934	conj	increase,limit
R9201	T12896	T12898	compound	type,amyloidosis
R9202	T12897	T12896	punct	-,type
R9203	T12898	T12894	pobj	amyloidosis,of
R9204	T12939	T12940	compound	Aβ,degradation
R9205	T12899	T12875	punct	.,demonstrate
R9206	T12901	T12902	advmod	However,are
R9207	T12940	T12938	dobj	degradation,increase
R9208	T12903	T12902	punct	", ",are
R9209	T12941	T12938	punct	", ",increase
R9210	T12904	T12902	prep	despite,are
R9211	T12905	T12906	amod	dramatic,reductions
R9212	T12906	T12904	pobj	reductions,despite
R9213	T12907	T12906	prep	in,reductions
R9214	T12908	T12909	compound	Aβ,synthesis
R9215	T12909	T12907	pobj	synthesis,in
R9216	T12942	T12938	cc	and,increase
R9217	T12910	T12902	punct	", ",are
R9218	T12911	T12912	amod	neuritic,plaques
R9219	T12943	T12938	conj	enhance,increase
R9220	T12912	T12902	nsubj	plaques,are
R9221	T12913	T12914	amod	stable,structures
R9222	T12914	T12902	attr	structures,are
R9223	T12944	T12943	dobj	phagocytosis,enhance
R9224	T12915	T12916	advmod	in,vivo
R9225	T12916	T12914	advmod	vivo,structures
R9226	T12945	T12944	prep	of,phagocytosis
R9227	T12917	T12918	dep	that,disaggregate
R9228	T12918	T12914	relcl	disaggregate,structures
R9229	T12919	T12918	aux	do,disaggregate
R9230	T12946	T12947	amod	deposited,amyloid
R9231	T12920	T12918	neg	not,disaggregate
R9232	T12921	T12918	advmod	quickly,disaggregate
R9233	T12947	T12945	pobj	amyloid,of
R9234	T12922	T12902	punct	.,are
R9235	T12948	T12928	aux	may,required
R9236	T12924	T12925	nsubj	It,is
R9237	T12926	T12925	acomp	possible,is
R9238	T12949	T12928	auxpass	be,required
R9239	T12927	T12928	mark	that,required
R9240	T12928	T12925	ccomp	required,is
R9241	T12929	T12930	det	a,combination
R9242	T12950	T12951	aux	to,reverse
R9243	T12930	T12928	nsubjpass	combination,required
R9244	T12931	T12930	prep	of,combination
R9245	T12951	T12928	advcl	reverse,required
R9246	T12932	T12931	pobj	therapies,of
R9247	T12933	T12934	aux	to,limit
R9248	T12952	T12951	dobj	damage,reverse
R9249	T12953	T12952	acl	associated,damage
R9250	T12954	T12953	prep	with,associated
R9251	T13040	T13028	auxpass	be,required
R9252	T12955	T12954	pobj	AD,with
R9253	T12956	T12925	punct	.,is
R9254	T13041	T13042	aux	to,determine
R9255	T13042	T13028	advcl	determine,required
R9256	T12958	T12959	advmod	However,provide
R9257	T13043	T13044	mark	whether,has
R9258	T13044	T13042	ccomp	has,determine
R9259	T13045	T13046	det	the,brain
R9260	T12960	T12959	punct	", ",provide
R9261	T13046	T13044	nsubj	brain,has
R9262	T13047	T13046	amod	human,brain
R9263	T13048	T13049	det	the,capacity
R9264	T12961	T12962	mark	if,started
R9265	T13049	T13044	dobj	capacity,has
R9266	T12962	T12959	advcl	started,provide
R9267	T13050	T13051	aux	to,repair
R9268	T13051	T13049	acl	repair,capacity
R9269	T12963	T12964	advmod	early,enough
R9270	T13052	T13053	npadvmod	amyloid,associated
R9271	T13053	T13055	amod	associated,damage
R9272	T13054	T13053	punct	-,associated
R9273	T12964	T12962	advmod	enough,started
R9274	T13055	T13051	dobj	damage,repair
R9275	T13056	T13055	prep	of,damage
R9276	T13057	T13056	pobj	AD,of
R9277	T12965	T12962	prep	in,started
R9278	T13058	T13059	mark	once,arrested
R9279	T13059	T13051	advcl	arrested,repair
R9280	T12966	T12967	det	the,course
R9281	T13060	T13061	det	the,progression
R9282	T13061	T13059	nsubjpass	progression,arrested
R9283	T13062	T13061	prep	of,progression
R9284	T12967	T12965	pobj	course,in
R9285	T13063	T13062	pobj	pathology,of
R9286	T13064	T13059	auxpass	is,arrested
R9287	T13065	T13028	punct	.,required
R9288	T12968	T12967	prep	of,course
R9289	T12969	T12968	pobj	disease,of
R9290	T12970	T12959	punct	", ",provide
R9291	T12971	T12972	compound	secretase,inhibitors
R9292	T12972	T12959	nsubj	inhibitors,provide
R9293	T12973	T12972	advmod	alone,inhibitors
R9294	T12974	T12959	aux	could,provide
R9295	T12975	T12976	amod	substantial,benefit
R9296	T12976	T12959	dobj	benefit,provide
R9297	T12977	T12976	prep	in,benefit
R9298	T12978	T12977	pcomp	slowing,in
R9299	T12979	T12980	amod	pathogenic,processes
R9300	T12980	T12978	dobj	processes,slowing
R9301	T12981	T12980	acl	linked,processes
R9302	T12982	T12981	prep	to,linked
R9303	T12983	T12984	compound	amyloid,deposition
R9304	T12984	T12982	pobj	deposition,to
R9305	T12985	T12959	punct	.,provide
R9306	T12987	T12988	advmod	Even,at
R9307	T12988	T12989	prep	at,suggests
R9308	T12990	T12991	amod	later,stages
R9309	T12991	T12988	pobj	stages,at
R9310	T12992	T12991	prep	in,stages
R9311	T12993	T12994	det	the,disease
R9312	T12994	T12992	pobj	disease,in
R9313	T12995	T12989	punct	", ",suggests
R9314	T12996	T12997	det	the,presence
R9315	T12997	T12989	nsubj	presence,suggests
R9316	T12998	T12997	prep	of,presence
R9317	T12999	T13000	amod	substantial,activation
R9318	T13000	T12998	pobj	activation,of
R9319	T13001	T13000	amod	microglial,activation
R9320	T13002	T12997	prep	in,presence
R9321	T13003	T13004	amod	human,AD
R9322	T13004	T13002	pobj	AD,in
R9323	T13005	T13006	punct	[,40
R9324	T13006	T13004	parataxis	40,AD
R9325	T13007	T13006	punct	],40
R9326	T13008	T13009	mark	that,allow
R9327	T13009	T12989	ccomp	allow,suggests
R9328	T13010	T13011	advmod	simply,slowing
R9329	T13011	T13009	csubj	slowing,allow
R9330	T13012	T13013	det	the,formation
R9331	T13013	T13011	dobj	formation,slowing
R9332	T13014	T13013	prep	of,formation
R9333	T13015	T13016	amod	new,deposits
R9334	T13016	T13014	pobj	deposits,of
R9335	T13017	T13016	compound	amyloid,deposits
R9336	T13018	T13009	aux	may,allow
R9337	T13019	T13020	amod	ongoing,phagocytosis
R9338	T13020	T13021	nsubj	phagocytosis,diminish
R9339	T13021	T13009	ccomp	diminish,allow
R9340	T13022	T13021	aux	to,diminish
R9341	T13023	T13024	amod	preexisting,lesions
R9342	T13024	T13021	dobj	lesions,diminish
R9343	T13025	T12989	punct	.,suggests
R9344	T13027	T13028	advmod	However,required
R9345	T13029	T13028	punct	", ",required
R9346	T13030	T13031	det	the,development
R9347	T13031	T13028	nsubjpass	development,required
R9348	T13032	T13031	prep	of,development
R9349	T13033	T13034	amod	safe,inhibitors
R9350	T13034	T13032	pobj	inhibitors,of
R9351	T13035	T13033	cc	and,safe
R9352	T13036	T13033	conj	effective,safe
R9353	T13037	T13034	compound	secretase,inhibitors
R9354	T13038	T13028	aux	will,required
R9355	T13039	T13028	advmod	ultimately,required
R1499	T2237	T2234	conj	AS,S36
R1575	T2317	T2315	dobj	offspring,crossing
R3687	T5268	T5265	parataxis	samples,Aβ
R2	T139	T137	prep	following,Amyloidosis
R3	T140	T139	pobj	Suppression,following
R4	T141	T140	prep	of,Suppression
R5	T142	T143	compound	Aβ,Production
R6	T143	T141	pobj	Production,of
R7	T144	T140	prep	in,Suppression
R8	T145	T146	det	a,Model
R11	T148	T146	prep	of,Model
R12	T149	T150	compound	Alzheimer,Disease
R13	T150	T148	pobj	Disease,of
R14	T155	T156	det	The,secretases
R18	T160	T156	punct	),secretases
R19	T161	T162	dep	that,cleave
R20	T162	T156	relcl	cleave,secretases
R21	T163	T164	nmod	amyloid,β
R28	T170	T162	prep	from,cleave
R29	T171	T172	det	the,protein
R33	T175	T172	punct	(,protein
R34	T176	T172	appos	APP,protein
R35	T177	T159	punct	),been
R36	T178	T159	aux	have,been
R37	T179	T180	det	the,focus
R38	T180	T159	attr	focus,been
R39	T181	T180	prep	of,focus
R40	T182	T183	amod	considerable,investigation
R41	T183	T181	pobj	investigation,of
R42	T184	T183	prep	in,investigation
R43	T185	T186	det	the,development
R44	T186	T184	pobj	development,in
R45	T187	T186	prep	of,development
R46	T188	T187	pobj	treatments,of
R47	T189	T188	prep	for,treatments
R48	T190	T191	compound	Alzheimer,disease
R49	T191	T189	pobj	disease,for
R50	T192	T159	punct	.,been
R51	T194	T195	det	The,prediction
R52	T195	T196	nsubj	prediction,been
R53	T197	T196	aux	has,been
R54	T198	T199	mark	that,facilitate
R78	T222	T223	det	the,repair
R79	T223	T199	dobj	repair,facilitate
R80	T224	T223	prep	of,repair
R81	T225	T226	amod	damaged,tissue
R82	T226	T224	pobj	tissue,of
R83	T227	T223	cc	and,repair
R84	T228	T223	conj	removal,repair
R85	T229	T228	prep	of,removal
R86	T230	T231	compound	amyloid,lesions
R87	T231	T229	pobj	lesions,of
R88	T232	T196	punct	.,been
R89	T234	T235	advmod	However,performed
R90	T236	T235	punct	", ",performed
R91	T237	T238	det	no,studies
R94	T240	T238	compound	term,studies
R96	T242	T238	acl	using,studies
R97	T243	T244	compound	animal,models
R98	T244	T242	dobj	models,using
R99	T245	T244	prep	of,models
R100	T246	T247	compound	amyloid,pathology
R101	T247	T245	pobj	pathology,of
R102	T248	T235	aux	have,performed
R103	T249	T235	advmod	yet,performed
R104	T250	T235	auxpass	been,performed
R105	T251	T252	aux	to,test
R106	T252	T235	advcl	test,performed
R107	T253	T254	det	this,hypothesis
R108	T254	T252	dobj	hypothesis,test
R109	T255	T235	punct	.,performed
R110	T258	T257	cc	and,Methods
R111	T259	T257	conj	Findings,Methods
R112	T261	T262	nsubj	We,generated
R113	T263	T262	aux	have,generated
R114	T264	T265	det	a,model
R118	T268	T269	dep	that,mimics
R121	T271	T272	det	the,arrest
R122	T272	T269	dobj	arrest,mimics
R123	T273	T272	prep	of,arrest
R124	T274	T275	compound	Aβ,production
R125	T275	T273	pobj	production,of
R126	T276	T275	acl	expected,production
R127	T277	T276	prep	from,expected
R128	T278	T277	pobj	treatment,from
R129	T279	T278	prep	with,treatment
R130	T280	T281	compound	secretase,inhibitors
R131	T281	T279	pobj	inhibitors,with
R132	T282	T262	punct	.,generated
R133	T284	T285	det	These,mice
R134	T285	T286	nsubj	mice,overexpress
R135	T287	T288	compound	mutant,APP
R136	T288	T286	dobj	APP,overexpress
R137	T289	T286	prep	from,overexpress
R138	T290	T291	det	a,vector
R139	T291	T289	pobj	vector,from
R140	T292	T293	dep	that,regulated
R144	T296	T293	agent	by,regulated
R145	T297	T296	pobj	doxycycline,by
R146	T298	T286	punct	.,overexpress
R147	T300	T301	prep	Under,induces
R148	T302	T303	amod	normal,conditions
R149	T303	T300	pobj	conditions,Under
R150	T304	T301	punct	", ",induces
R151	T305	T306	amod	high,level
R154	T308	T301	nsubj	expression,induces
R155	T309	T308	prep	of,expression
R156	T310	T309	pobj	APP,of
R157	T311	T301	advmod	quickly,induces
R158	T312	T313	amod	fulminant,pathology
R161	T315	T301	punct	.,induces
R162	T317	T318	nsubj	We,show
R163	T319	T320	mark	that,inhibits
R167	T323	T324	amod	transgenic,expression
R170	T326	T320	prep	by,inhibits
R171	T327	T328	amod	greater,95
R174	T330	T326	pobj	%,by
R175	T331	T320	cc	and,inhibits
R176	T332	T320	conj	reduces,inhibits
R177	T333	T334	compound	Aβ,production
R178	T334	T332	dobj	production,reduces
R179	T335	T332	prep	to,reduces
R180	T336	T335	pobj	levels,to
R181	T337	T336	acl	found,levels
R182	T338	T337	prep	in,found
R183	T339	T340	amod	nontransgenic,mice
R184	T340	T338	pobj	mice,in
R185	T341	T318	punct	.,show
R186	T343	T344	nsubj	Suppression,halts
R187	T345	T343	prep	of,Suppression
R188	T346	T347	amod	transgenic,synthesis
R191	T349	T343	prep	in,Suppression
R192	T350	T351	det	this,model
R193	T351	T349	pobj	model,in
R194	T352	T344	advmod	abruptly,halts
R195	T353	T354	det	the,progression
R196	T354	T344	dobj	progression,halts
R197	T355	T354	prep	of,progression
R198	T356	T357	compound	amyloid,pathology
R199	T357	T355	pobj	pathology,of
R200	T358	T344	punct	.,halts
R201	T360	T361	advmod	However,appear
R202	T362	T361	punct	", ",appear
R203	T363	T361	nsubj	formation,appear
R204	T364	T363	cc	and,formation
R205	T365	T363	conj	disaggregation,formation
R206	T366	T363	prep	of,formation
R207	T367	T368	compound	amyloid,deposits
R208	T368	T366	pobj	deposits,of
R209	T369	T370	aux	to,be
R210	T370	T361	xcomp	be,appear
R211	T371	T370	prep	in,be
R212	T372	T371	pobj	disequilibrium,in
R213	T373	T374	mark	as,require
R217	T377	T378	advmod	far,longer
R218	T378	T374	advmod	longer,require
R219	T379	T380	aux	to,disperse
R220	T380	T374	xcomp	disperse,require
R221	T381	T382	mark	than,assemble
R224	T384	T361	punct	.,appear
R225	T386	T387	nsubj	Mice,retain
R226	T388	T389	prep	in,suppressed
R232	T394	T389	prep	for,suppressed
R233	T395	T396	advmod	as,6
R237	T399	T394	pobj	mo,for
R238	T400	T389	prep	after,suppressed
R239	T401	T402	det	the,formation
R240	T402	T400	pobj	formation,after
R241	T403	T402	prep	of,formation
R242	T404	T405	compound	Aβ,deposits
R243	T405	T403	pobj	deposits,of
R244	T406	T407	det	a,load
R248	T410	T387	punct	", ",retain
R249	T411	T387	prep	with,retain
R250	T412	T413	amod	little,sign
R251	T413	T411	pobj	sign,with
R252	T414	T413	prep	of,sign
R253	T415	T416	amod	active,clearance
R254	T416	T414	pobj	clearance,of
R255	T417	T387	punct	.,retain
R256	T421	T422	det	This,study
R257	T422	T423	nsubj	study,demonstrates
R258	T424	T425	mark	that,are
R265	T431	T432	advmod	highly,stable
R266	T432	T433	amod	stable,structures
R267	T433	T425	attr	structures,are
R268	T434	T435	advmod	in,vivo
R269	T435	T433	advmod	vivo,structures
R270	T436	T437	dep	that,are
R271	T437	T433	relcl	are,structures
R272	T438	T437	acomp	slow,are
R273	T439	T440	aux	to,disaggregate
R274	T440	T438	xcomp	disaggregate,slow
R275	T441	T423	punct	.,demonstrates
R276	T443	T444	poss	Our,findings
R277	T444	T445	nsubj	findings,suggest
R278	T446	T447	mark	that,halt
R289	T457	T458	det	the,progression
R290	T458	T447	dobj	progression,halt
R291	T459	T458	prep	of,progression
R292	T460	T459	pobj	pathology,of
R293	T461	T447	punct	", ",halt
R294	T462	T447	cc	but,halt
R295	T463	T464	mark	that,be
R300	T468	T464	acomp	imperative,be
R301	T469	T464	punct	", ",be
R302	T470	T471	mark	as,appears
R305	T473	T474	mark	that,require
R314	T482	T483	amod	additional,intervention
R315	T483	T474	dobj	intervention,require
R316	T484	T485	aux	to,clear
R317	T485	T483	advcl	clear,intervention
R318	T486	T445	punct	.,suggest
R2327	T3419	T3416	parataxis	17,22C11
R1141	T1769	T1774	nsubj	fragment,cloned
R1172	T1801	T1799	pobj	fragment,to
R1395	T2032	T2029	compound	KS,vector
R471	T861	T859	pobj	approaches,of
R622	T1020	T1017	appos	enzyme,enzymes
R8460	T12128	T12127	nummod	50,55
R8796	T12483	T12482	punct	-,off
R8802	T12488	T12489	punct	[,32
R8803	T12489	T12486	parataxis	32,Huntington
R8804	T12490	T12489	punct	],32
R8808	T12493	T12494	punct	[,62
R9120	T12833	T12832	compound	APP,synthesis
R9127	T12838	T12839	amod	additional,time
R9128	T12839	T12837	dobj	time,requires
R5135	T7755	T7752	prep	via,expressing
R5416	T8054	T8051	appos	mo,mo
R5417	T8055	T8051	amod	treated,mo
R5418	T8056	T8057	punct	(,5
R6074	T8753	T8752	amod	aggregated,Aβ
R6077	T8755	T8754	pobj	either,as
R6078	T8756	T8755	prep	of,either
R6081	T8758	T8756	pobj	groups,of
R6413	T9109	T9110	det	any,changes
R6414	T9110	T9107	attr	changes,were
R6566	T9276	T9264	punct	", ",seen
R6567	T9277	T9264	cc	and,seen
R6854	T9583	T9584	advmod	However,remained
R6873	T9661	T9654	dobj	levels,showed
R6875	T9662	T9661	amod	similar,levels
R6876	T9663	T9662	prep	to,similar
R6878	T9664	T9665	det	the,controls
R6879	T9665	T9663	pobj	controls,to
R6881	T9666	T9665	amod	untreated,controls
R6882	T9667	T9668	punct	(,7C
R6884	T9668	T9654	parataxis	7C,showed
R6885	T9669	T9668	compound	Figure,7C
R6887	T9670	T9668	punct	),7C
R6888	T9671	T9654	punct	.,showed
R6890	T9673	T9674	advmod	Intriguingly,showed
R6891	T9675	T9674	punct	", ",showed
R6893	T9676	T9674	nsubj	all,showed
R6894	T9677	T9676	prep	of,all
R6896	T9678	T9679	det	the,animals
R6897	T9679	T9677	pobj	animals,of
R6898	T9680	T9681	npadvmod	dox,reared
R6900	T9681	T9679	amod	reared,animals
R6901	T9682	T9681	punct	-,reared
R6902	T9683	T9676	punct	", ",all
R6904	T9684	T9685	preconj	both,transgenic
R6905	T9685	T9676	amod	transgenic,all
R6907	T9686	T9685	cc	and,transgenic
R6908	T9687	T9688	amod	wild,type
R6909	T9688	T9685	conj	type,transgenic
R6911	T9689	T9688	punct	-,type
R6912	T9690	T9674	punct	", ",showed
R6914	T9691	T9692	amod	altered,rhythms
R6915	T9692	T9674	dobj	rhythms,showed
R6916	T9693	T9692	amod	circadian,rhythms
R6917	T9694	T9692	prep	with,rhythms
R6919	T9695	T9696	advmod	far,less
R6920	T9696	T9697	amod	less,distinction

2_test

Id	Subject	Object	Predicate	Lexical cue
16279840-8939850-86242765	21397-21399	8939850	denotes	16
16279840-1566067-86242742	2467-2468	1566067	denotes	1
16279840-12142353-86242743	2764-2765	12142353	denotes	2
16279840-12847300-86242744	2766-2767	12847300	denotes	3
16279840-12847155-86242745	3283-3284	12847155	denotes	4
16279840-12765607-86242746	3483-3484	12765607	denotes	6
16279840-12640446-86242747	3485-3486	12640446	denotes	7
16279840-12052174-86242748	4095-4096	12052174	denotes	8
16279840-12415246-86242749	4097-4098	12415246	denotes	9
16279840-15102495-86242750	4192-4194	15102495	denotes	11
16279840-1319065-86242751	5075-5077	1319065	denotes	12
16279840-7937760-86242752	5078-5080	7937760	denotes	13
16279840-8855286-86242753	5249-5251	8855286	denotes	14
16279840-9117892-86242754	5703-5705	9117892	denotes	15
16279840-8939850-86242755	8610-8612	8939850	denotes	16
16279840-2649245-86242756	12442-12444	2649245	denotes	17
16279840-8058797-86242757	12620-12622	8058797	denotes	18
16279840-8331390-86242758	12701-12703	8331390	denotes	19
16279840-6326095-86242759	13956-13958	6326095	denotes	20
16279840-2422580-86242760	14734-14736	2422580	denotes	21
16279840-12218651-86242761	17968-17970	12218651	denotes	22
16279840-11160418-86242762	19133-19135	11160418	denotes	23
16279840-8191290-86242762	19133-19135	8191290	denotes	23
16279840-7706234-86242762	19133-19135	7706234	denotes	23
16279840-1319065-86242763	21253-21255	1319065	denotes	12
16279840-7937760-86242764	21256-21258	7937760	denotes	13
16279840-9117892-86242766	21704-21706	9117892	denotes	15
16279840-14645205-86242767	21707-21709	14645205	denotes	26
16279840-9354339-86242768	21710-21712	9354339	denotes	27
16279840-8939850-86242769	30884-30886	8939850	denotes	16
16279840-7835246-86242770	30887-30889	7835246	denotes	28
16279840-9354339-86242771	31407-31409	9354339	denotes	27
16279840-10493755-86242772	31822-31824	10493755	denotes	29
16279840-12218651-86242773	39497-39499	12218651	denotes	22
16279840-11160418-86242774	40439-40441	11160418	denotes	23
16279840-12151510-86242775	45569-45571	12151510	denotes	30
16279840-15755686-86242776	45974-45976	15755686	denotes	31
16279840-10778856-86242777	49619-49621	10778856	denotes	32
16279840-8810256-86242778	49903-49905	8810256	denotes	33
16279840-11279122-86242779	49920-49922	11279122	denotes	34
16279840-14709552-86242780	50061-50063	14709552	denotes	35
16279840-15452193-86242781	50064-50066	15452193	denotes	36
16279840-9354339-86242782	52144-52146	9354339	denotes	27
16279840-12404061-86242783	53996-53998	12404061	denotes	37
16279840-15353209-86242783	53996-53998	15353209	denotes	37
16279840-11164276-86242783	53996-53998	11164276	denotes	37
16279840-15191802-86242783	53996-53998	15191802	denotes	37
16279840-15191802-86242784	54157-54159	15191802	denotes	40
16279840-11896164-86242785	54558-54560	11896164	denotes	41
16279840-10908610-86242785	54558-54560	10908610	denotes	41
16279840-12930788-86242785	54558-54560	12930788	denotes	41
16279840-12897211-86242786	54694-54696	12897211	denotes	44
16279840-11700559-86242786	54694-54696	11700559	denotes	44
16279840-12805356-86242786	54694-54696	12805356	denotes	44
16279840-15448688-86242786	54694-54696	15448688	denotes	44
16279840-9861045-86242787	55448-55450	9861045	denotes	48
16279840-12404061-86242788	55862-55864	12404061	denotes	37
16279840-15353209-86242788	55862-55864	15353209	denotes	37
16279840-11164276-86242788	55862-55864	11164276	denotes	37
16279840-15191802-86242788	55862-55864	15191802	denotes	37
16279840-15294141-86242789	56287-56289	15294141	denotes	49
16279840-12736345-86242789	56287-56289	12736345	denotes	49
16279840-11231639-86242789	56287-56289	11231639	denotes	49
16279840-10932230-86242790	56504-56506	10932230	denotes	52
16279840-15240806-86242791	56507-56509	15240806	denotes	53
16279840-12657655-86242792	56676-56678	12657655	denotes	54
16279840-12736345-86242793	56880-56882	12736345	denotes	50
16279840-10932230-86242794	56883-56885	10932230	denotes	52
16279840-14751766-86242795	56886-56888	14751766	denotes	55
16279840-13679422-86242796	57128-57130	13679422	denotes	56
16279840-12223540-86242797	57131-57133	12223540	denotes	57
16279840-11329064-86242798	57791-57793	11329064	denotes	58
16279840-11755009-86242799	57839-57841	11755009	denotes	59
16279840-15473997-86242800	57842-57844	15473997	denotes	60
16279840-15294141-86242801	58168-58170	15294141	denotes	49
16279840-16020737-86242802	58461-58463	16020737	denotes	61
16279840-10778856-86242803	58809-58811	10778856	denotes	32
16279840-16020737-86242804	59615-59617	16020737	denotes	61
16279840-15191802-86242805	61785-61787	15191802	denotes	40
16279840-9117892-86242806	62658-62660	9117892	denotes	15
16279840-8938131-86242807	62661-62663	8938131	denotes	63
T68795	2467-2468	1566067	denotes	1
T49885	2764-2765	12142353	denotes	2
T3088	2766-2767	12847300	denotes	3
T25848	3283-3284	12847155	denotes	4
T33124	3483-3484	12765607	denotes	6
T54871	3485-3486	12640446	denotes	7
T59505	4095-4096	12052174	denotes	8
T70543	4097-4098	12415246	denotes	9
T21403	4192-4194	15102495	denotes	11
T62623	5075-5077	1319065	denotes	12
T61771	5078-5080	7937760	denotes	13
T70705	5249-5251	8855286	denotes	14
T80824	5703-5705	9117892	denotes	15
T66109	8610-8612	8939850	denotes	16
T97629	12442-12444	2649245	denotes	17
T90408	12620-12622	8058797	denotes	18
T66974	12701-12703	8331390	denotes	19
T72572	13956-13958	6326095	denotes	20
T67755	14734-14736	2422580	denotes	21
T23633	17968-17970	12218651	denotes	22
T91382	19133-19135	11160418	denotes	23
T2274	19133-19135	8191290	denotes	23
T73715	19133-19135	7706234	denotes	23
T47654	21253-21255	1319065	denotes	12
T45338	21256-21258	7937760	denotes	13
T42231	21397-21399	8939850	denotes	16
T248	21704-21706	9117892	denotes	15
T42200	21707-21709	14645205	denotes	26
T15428	21710-21712	9354339	denotes	27
T30577	30884-30886	8939850	denotes	16
T87295	30887-30889	7835246	denotes	28
T15927	31407-31409	9354339	denotes	27
T56717	31822-31824	10493755	denotes	29
T30455	39497-39499	12218651	denotes	22
T72545	40439-40441	11160418	denotes	23
T85669	45569-45571	12151510	denotes	30
T73868	45974-45976	15755686	denotes	31
T17182	49619-49621	10778856	denotes	32
T35842	49903-49905	8810256	denotes	33
T45931	49920-49922	11279122	denotes	34
T75343	50061-50063	14709552	denotes	35
T70534	50064-50066	15452193	denotes	36
T85491	52144-52146	9354339	denotes	27
T39948	53996-53998	12404061	denotes	37
T66452	53996-53998	15353209	denotes	37
T47811	53996-53998	11164276	denotes	37
T18572	53996-53998	15191802	denotes	37
T58634	54157-54159	15191802	denotes	40
T83416	54558-54560	11896164	denotes	41
T54064	54558-54560	10908610	denotes	41
T75448	54558-54560	12930788	denotes	41
T36841	54694-54696	12897211	denotes	44
T24141	54694-54696	11700559	denotes	44
T67406	54694-54696	12805356	denotes	44
T45503	54694-54696	15448688	denotes	44
T6243	55448-55450	9861045	denotes	48
T28273	55862-55864	12404061	denotes	37
T59342	55862-55864	15353209	denotes	37
T92299	55862-55864	11164276	denotes	37
T40804	55862-55864	15191802	denotes	37
T6534	56287-56289	15294141	denotes	49
T25436	56287-56289	12736345	denotes	49
T73130	56287-56289	11231639	denotes	49
T95188	56504-56506	10932230	denotes	52
T32095	56507-56509	15240806	denotes	53
T53094	56676-56678	12657655	denotes	54
T47250	56880-56882	12736345	denotes	50
T49423	56883-56885	10932230	denotes	52
T67630	56886-56888	14751766	denotes	55
T24008	57128-57130	13679422	denotes	56
T10578	57131-57133	12223540	denotes	57
T22755	57791-57793	11329064	denotes	58
T78765	57839-57841	11755009	denotes	59
T2807	57842-57844	15473997	denotes	60
T86071	58168-58170	15294141	denotes	49
T97521	58461-58463	16020737	denotes	61
T40745	58809-58811	10778856	denotes	32
T43043	59615-59617	16020737	denotes	61
T8156	61785-61787	15191802	denotes	40
T75078	62658-62660	9117892	denotes	15
T86129	62661-62663	8938131	denotes	63

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T75	48-50	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T76	48-61	GO:0034205	denotes	Aβ Production
T77	67-77	SO_EXT:transgenic_entity	denotes	Transgenic
T78	169-178	GO_EXT:0008233	denotes	proteases
T79	197-203	MOP:0000780	denotes	cleave
T80	204-213	CHEBI_PR_EXT:amyloid_beta	denotes	amyloid-β
T81	215-217	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T82	215-217	_FRAGMENT	denotes	Aβ
T83	219-226	CHEBI_PR_EXT:amyloid_beta	denotes	peptide
T84	219-226	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T85	236-243	CHEBI_PR_EXT:amyloid_beta	denotes	amyloid
T86	236-261	PR_EXT:000004168	denotes	amyloid precursor protein
T87	254-261	CHEBI_PR_EXT:protein	denotes	protein
T88	263-266	PR_EXT:000004168	denotes	APP
T89	412-414	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T90	412-425	GO:0034205	denotes	Aβ production
T91	433-438	UBERON:0000955	denotes	brain
T92	565-571	UBERON:0000479	denotes	tissue
T93	587-594	CHEBI:60425	denotes	amyloid
T94	640-646	NCBITaxon:33208	denotes	animal
T95	657-664	CHEBI:60425	denotes	amyloid
T96	766-776	SO_EXT:transgenic_entity	denotes	transgenic
T97	777-782	NCBITaxon:10088	denotes	mouse
T98	794-805	SO_EXT:0000704	denotes	genetically
T99	827-829	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T100	827-840	GO:0034205	denotes	Aβ production
T101	880-890	CHEBI_EXT:35222	denotes	inhibitors
T102	898-902	NCBITaxon:10088	denotes	mice
T103	907-914	GO:0010467	denotes	express
T104	915-921	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T105	922-925	PR_EXT:000004168	denotes	APP
T106	933-939	SO_EXT:0000440	denotes	vector
T107	952-961	GO:0065007	denotes	regulated
T108	965-976	CHEBI:50845	denotes	doxycycline
T109	1014-1024	GO:0010467	denotes	expression
T110	1028-1031	PR_EXT:000004168	denotes	APP
T111	1058-1065	CHEBI:60425	denotes	amyloid
T112	1090-1101	CHEBI:50845	denotes	doxycycline
T113	1126-1136	SO_EXT:transgenic_entity	denotes	transgenic
T114	1137-1140	PR_EXT:000004168	denotes	APP
T115	1141-1151	GO:0010467	denotes	expression
T116	1184-1186	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T117	1184-1197	GO:0034205	denotes	Aβ production
T118	1220-1230	SO_EXT:transgenic_entity	denotes	transgenic
T119	1231-1235	NCBITaxon:10088	denotes	mice
T120	1252-1262	SO_EXT:transgenic_entity	denotes	transgenic
T121	1263-1265	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T122	1263-1275	GO:0034205	denotes	Aβ synthesis
T123	1324-1331	CHEBI:60425	denotes	amyloid
T124	1384-1391	CHEBI:60425	denotes	amyloid
T125	1496-1500	NCBITaxon:10088	denotes	Mice
T126	1510-1513	PR_EXT:000004168	denotes	APP
T127	1510-1523	GO:0042983	denotes	APP synthesis
T128	1582-1584	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T129	1616-1623	CHEBI:60425	denotes	amyloid
T130	1709-1716	CHEBI:60425	denotes	amyloid
T131	1728-1738	SO_EXT:transgenic_entity	denotes	transgenic
T132	1739-1743	NCBITaxon:10088	denotes	mice
T133	1848-1850	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T134	1848-1861	GO:0034205	denotes	Aβ production
T135	2002-2009	CHEBI:60425	denotes	amyloid
T611	2286-2293	CHEBI:60425	denotes	amyloid
T612	2348-2357	CHEBI_PR_EXT:amyloid_beta	denotes	amyloid-β
T613	2359-2361	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T614	2363-2370	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T615	2515-2522	SO_EXT:0000704	denotes	genetic
T616	2557-2566	SO_EXT:sequence_alteration_entity_or_process	denotes	mutations
T617	2574-2581	CHEBI_PR_EXT:amyloid_beta	denotes	amyloid
T618	2574-2599	PR_EXT:000004168	denotes	amyloid precursor protein
T619	2592-2599	CHEBI_PR_EXT:protein	denotes	protein
T620	2601-2604	PR_EXT:000004168	denotes	APP
T621	2618-2620	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T622	2637-2650	PR_EXT:000013344	denotes	presenilins 1
T623	2637-2648	_FRAGMENT	denotes	presenilins
T624	2655-2656	PR_EXT:000013345	denotes	2
T625	2700-2712	GO_EXT:0004175	denotes	endoprotease
T626	2713-2720	GO:0032991	denotes	complex
T627	2726-2734	GO_EXT:biological_release	denotes	releases
T628	2735-2737	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T629	2743-2746	PR_EXT:000004168	denotes	APP
T630	2793-2809	GO:0034205	denotes	production of Aβ
T631	2807-2809	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T632	2901-2903	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T633	2964-2971	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T634	3024-3032	GO:0042571	denotes	antibody
T635	3070-3072	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T636	3103-3109	NCBITaxon:9606	denotes	humans
T637	3156-3163	CHEBI:60425	denotes	amyloid
T638	3346-3353	CHEBI:60425	denotes	amyloid
T639	3388-3397	GO:0050890	denotes	cognitive
T640	3461-3463	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T641	3473-3481	GO:0042571	denotes	antibody
T642	3544-3554	GO:0042571	denotes	antibodies
T643	3573-3580	CHEBI_SO_EXT:epitope	denotes	epitope
T644	3604-3609	UBERON:0000955	denotes	brain
T645	3775-3777	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T646	3831-3838	CHEBI_PR_EXT:protein	denotes	protein
T647	3839-3842	PR_EXT:000004168	denotes	APP
T648	3844-3851	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	Peptide
T649	3852-3854	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T650	3858-3866	GO_EXT:biological_release	denotes	released
T651	3872-3875	PR_EXT:000004168	denotes	APP
T652	3897-3904	CHEBI_GO_SO_EXT:enzyme	denotes	enzymes
T653	3910-3933	PR_EXT:000004615	denotes	β-APP cleaving enzyme 1
T654	3916-3924	MOP:0000780	denotes	cleaving
T655	3925-3931	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T656	3935-3940	PR_EXT:000004615	denotes	BACE1
T657	3946-3957	GO:0070765	denotes	γ-secretase
T658	3965-3971	MOP:0000780	denotes	cleave
T659	3976-3987	CHEBI:33837	denotes	holoprotein
T660	3995-3997	_FRAGMENT	denotes	N-
T661	4004-4011	CHEBI_SO_EXT:N_terminus_or_N_terminal_region	denotes	termini
T662	4002-4011	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-termini
T663	4015-4017	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T664	4041-4051	CHEBI_EXT:35222	denotes	inhibitors
T665	4055-4066	GO:0070765	denotes	γ-secretase
T666	4111-4119	CHEBI:36357	denotes	molecule
T667	4120-4130	CHEBI_EXT:35222	denotes	inhibitors
T668	4134-4157	PR_EXT:000004615	denotes	β-APP cleaving enzyme 1
T669	4140-4148	MOP:0000780	denotes	cleaving
T670	4149-4155	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T671	4252-4258	NCBITaxon:9606	denotes	humans
T672	4326-4349	PR_EXT:000004615	denotes	β-APP cleaving enzyme 1
T673	4332-4340	MOP:0000780	denotes	cleaving
T674	4341-4347	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T675	4401-4411	SO_EXT:transgenic_entity	denotes	transgenic
T676	4412-4417	NCBITaxon:10088	denotes	mouse
T677	4516-4521	UBERON:0000955	denotes	brain
T678	4545-4552	CHEBI:60425	denotes	amyloid
T679	4567-4583	GO:0034205	denotes	production of Aβ
T680	4581-4583	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T681	4618-4625	CHEBI:60425	denotes	amyloid
T682	4677-4683	NCBITaxon:33208	denotes	animal
T683	4704-4712	CHEBI_EXT:33893	denotes	reagents
T684	4750-4758	CHEBI_EXT:chemical_substance_or_polyatomic_entity	denotes	chemical
T685	4769-4779	CHEBI_EXT:35222	denotes	inhibitors
T686	4801-4806	NCBITaxon:9606	denotes	human
T687	4866-4871	NCBITaxon:10088	denotes	mouse
T688	4896-4903	CHEBI:60425	denotes	amyloid
T689	4909-4918	GO:0010467	denotes	expresses
T690	4921-4933	GO:0065007	denotes	controllable
T691	4934-4937	PR_EXT:000004168	denotes	APP
T692	4938-4947	SO_EXT:0000902	denotes	transgene
T693	5007-5016	GO:0065007	denotes	regulated
T694	5031-5043	CHEBI:27902	denotes	tetracycline
T695	5060-5064	CHEBI_EXT:33290	denotes	food
T696	5068-5073	CHEBI:15377	denotes	water
T697	5094-5104	GO:0010467	denotes	expression
T698	5138-5145	SO_EXT:0000440	denotes	vectors
T699	5188-5198	GO:0010467	denotes	expression
T700	5235-5247	CHEBI:27902	denotes	tetracycline
T701	5309-5323	CHEBI_EXT:52217	denotes	pharmaceutical
T702	5324-5333	CHEBI_EXT:35222	denotes	inhibitor
T703	5337-5339	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T704	5337-5350	GO:0034205	denotes	Aβ production
T705	5383-5390	CHEBI:60425	denotes	amyloid
T1438	5493-5502	SO_EXT:0000902	denotes	Transgene
T1439	5529-5541	CHEBI:27902	denotes	tetracycline
T1440	5562-5567	NCBITaxon:10088	denotes	mouse
T1441	5568-5573	NCBITaxon:9606	denotes	human
T1442	5606-5612	SO_EXT:0000440	denotes	vector
T1443	5616-5625	SO_EXT:sequence_substitution_process	denotes	replacing
T1444	5630-5645	SO_EXT:0000167	denotes	promoter region
T1445	5653-5658	PR_EXT:P04925	denotes	moPrP
T1446	5664-5670	SO_EXT:0000440	denotes	vector
T1447	5717-5729	CHEBI:27902	denotes	tetracycline
T1448	5741-5749	SO_EXT:0000167	denotes	promoter
T1449	5830-5838	GO:0006266	denotes	ligating
T1450	5839-5844	NCBITaxon:10088	denotes	mouse
T1451	5845-5848	PR_EXT:000004168	denotes	APP
T1452	5866-5868	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T1453	5869-5875	SO_EXT:0000417	denotes	domain
T1454	5877-5879	NCBITaxon:10088	denotes	mo
T1455	5890-5894	SO_EXT:cDNA	denotes	cDNA
T1456	5908-5914	SO_EXT:0000440	denotes	vector
T1457	5928-5935	SO_EXT:sequence_cloning_process	denotes	cloning
T1458	5940-5952	CHEBI:27902	denotes	tetracycline
T1459	5964-5972	SO_EXT:0000167	denotes	promoter
T1460	5974-5976	SO_EXT:0000028	denotes	bp
T1461	6013-6020	SO_EXT:0000112	denotes	primers
T1462	6041-6046	PR_EXT:P23940	denotes	BamHI
T1463	6082-6087	PR_EXT:P23940	denotes	BamHI
T1464	6134-6139	PR_EXT:P23940	denotes	BamHI
T1465	6157-6165	SO_EXT:0000167	denotes	promoter
T1466	6167-6174	SO:0001030	denotes	forward
T1467	6231-6238	SO:0001031	denotes	reverse
T1468	6307-6312	PR_EXT:P04925	denotes	moPrP
T1469	6318-6324	SO_EXT:0000188	denotes	intron
T1470	6347-6354	SO_EXT:0000112	denotes	primers
T1471	6378-6383	PR_EXT:P23940	denotes	BamHI
T1472	6406-6410	SO_EXT:0000147	denotes	exon
T1473	6448-6452	SO_EXT:0000147	denotes	exon
T1474	6456-6463	SO:0001030	denotes	forward
T1475	6505-6512	SO:0001031	denotes	reverse
T1476	6560-6561	CHEBI_SO_EXT:base	denotes	b
T1477	6562-6568	SO_EXT:0000188	denotes	intron
T1478	6582-6588	SO_EXT:sequence_cloning_process	denotes	cloned
T1479	6599-6606	SO_EXT:sequence_cloning_process	denotes	cloning
T1480	6607-6613	SO_EXT:0000440	denotes	vector
T1481	6670-6677	SO_EXT:sequence_deletion_process	denotes	excised
T1482	6702-6709	GO:0006266	denotes	ligated
T1483	6722-6723	CHEBI_SO_EXT:base	denotes	b
T1484	6743-6748	PR_EXT:P04925	denotes	moPrP
T1485	6765-6769	SO_EXT:0000147	denotes	exon
T1486	6773-6777	SO_EXT:0000147	denotes	exon
T1487	6785-6791	SO_EXT:0000205	denotes	3′ UTR
T1488	6837-6843	SO_EXT:0000440	denotes	vector
T1489	6859-6864	SO_EXT:0000147	denotes	exons
T1490	6879-6885	SO_EXT:0000188	denotes	intron
T1491	6893-6901	SO_EXT:0000167	denotes	promoter
T1492	6908-6914	SO_EXT:0000440	denotes	vector
T1493	6938-6943	PR_EXT:P23940	denotes	BamHI
T1494	6967-6973	SO_EXT:0000188	denotes	intron
T1495	6974-6981	SO_EXT:sequence_cloning_process	denotes	cloning
T1496	6982-6988	SO_EXT:0000112	denotes	primer
T1497	6994-7001	GO:0006266	denotes	ligated
T1498	7014-7015	CHEBI_SO_EXT:base	denotes	b
T1499	7016-7021	PR_EXT:P23940	denotes	BamHI
T1500	7022-7025	MOP:0000780	denotes	cut
T1501	7026-7038	CHEBI:27902	denotes	tetracycline
T1502	7039-7047	SO_EXT:0000167	denotes	promoter
T1503	7063-7071	GO:0006266	denotes	ligation
T1504	7089-7090	CHEBI_SO_EXT:base	denotes	b
T1505	7091-7097	SO_EXT:0000440	denotes	vector
T1506	7098-7106	SO_EXT:sequence_coding_function	denotes	encoding
T1507	7111-7123	CHEBI:27902	denotes	tetracycline
T1508	7124-7132	SO_EXT:0000167	denotes	promoter
T1509	7158-7163	SO_EXT:0000147	denotes	exons
T1510	7169-7175	SO_EXT:0000188	denotes	intron
T1511	7194-7200	SO_EXT:0000205	denotes	3′ UTR
T1512	7208-7213	PR_EXT:P04925	denotes	moPrP
T1513	7219-7225	SO_EXT:0000440	denotes	vector
T1514	7258-7265	SO_EXT:sequence_cloning_process	denotes	cloning
T1515	7266-7272	SO_EXT:0000440	denotes	vector
T1516	7336-7345	SO_EXT:sequence_alteration_entity_or_process	denotes	mutations
T1517	7355-7357	NCBITaxon:10088	denotes	mo
T1518	7367-7371	SO_EXT:cDNA	denotes	cDNA
T1519	7403-7409	SO_EXT:0000112	denotes	primer
T1520	7505-7512	SO_EXT:0000112	denotes	primers
T1521	7518-7524	SO_EXT:sequence_coding_function	denotes	encode
T1522	7537-7546	SO_EXT:sequence_alteration_entity_or_process	denotes	mutations
T1523	7556-7563	SO:0001030	denotes	forward
T1524	7621-7628	SO:0001031	denotes	reverse
T1525	7669-7676	SO:0001030	denotes	forward
T1526	7714-7721	SO:0001031	denotes	reverse
T1527	7732-7744	SO_EXT:0000006	denotes	PCR products
T1528	7750-7757	GO:0006266	denotes	ligated
T1529	7792-7798	SO_EXT:sequence_cloning_process	denotes	cloned
T1530	7822-7824	NCBITaxon:10088	denotes	mo
T1531	7840-7846	SO_EXT:0000440	denotes	vector
T1532	7919-7924	PR_EXT:P04925	denotes	moPrP
T1533	7930-7936	SO_EXT:0000440	denotes	vector
T1534	7964-7973	SO_EXT:engineered_biological_sequence	denotes	construct
T2122	7976-7986	GO:0045120	denotes	Pronuclear
T2123	8054-8059	PR_EXT:P04925	denotes	moPrP
T2124	8065-8067	NCBITaxon:10088	denotes	mo
T2125	8084-8090	SO_EXT:0000440	denotes	vector
T2126	8095-8105	SO_EXT:sequence_linearization	denotes	linearized
T2127	8133-8140	SO_EXT:sequence_deletion_process	denotes	excised
T2128	8178-8184	SO_EXT:0000440	denotes	vector
T2129	8207-8217	GO:0045120	denotes	pronucleus
T2130	8221-8231	GO:0009566	denotes	fertilized
T2131	8232-8236	CL:0000025	denotes	eggs
T2132	8262-8269	GO:0007618	denotes	matings
T2133	8279-8286	NCBITaxon:33208	denotes	animals
T2134	8325-8334	SO_EXT:0000902	denotes	transgene
T2135	8370-8373	PR_EXT:000001886	denotes	PrP
T2136	8376-8379	PR_EXT:000001886	denotes	PrP
T2137	8383-8390	SO_EXT:0000112	denotes	primers
T2138	8408-8417	SO_EXT:0000902	denotes	Transgene
T2139	8441-8445	GO_EXT:breeding	denotes	bred
T2140	8449-8456	NCBITaxon:33208	denotes	animals
T2142	8472-8484	CHEBI:27902	denotes	tetracycline
T2143	8512-8519	GO:0065007	denotes	control
T2144	8527-8534	CHEBI_EXT:calcium	denotes	calcium
T2145	8527-8552	GO_EXT:0004683	denotes	calcium-calmodulin kinase
T2146	8527-8556	PR_EXT:000003199	denotes	calcium-calmodulin kinase IIα
T2147	8558-8565	PR_EXT:000003199	denotes	CaMKIIα
T2148	8567-8575	SO_EXT:0000167	denotes	promoter
T2149	8674-8680	PR_EXT:000003199	denotes	Camk2a
T2150	8749-8759	SO_EXT:transgenic_entity	denotes	transgenic
T2151	8768-8771	PR_EXT:000004168	denotes	APP
T2152	8803-8806	PR_EXT:000004168	denotes	APP
T2153	8818-8822	NCBITaxon:10088	denotes	mice
T2154	8843-8847	CHEBI_EXT:33290	denotes	food
T2155	8852-8857	CHEBI:15377	denotes	water
T2156	8870-8876	NCBITaxon:33208	denotes	Animal
T2157	8995-9001	NCBITaxon:33208	denotes	Animal
T2390	9028-9039	CHEBI:50845	denotes	Doxycycline
T2391	9055-9066	CHEBI:50845	denotes	Doxycycline
T2392	9068-9071	CHEBI:50845	denotes	dox
T2393	9121-9124	CHEBI:50845	denotes	dox
T2394	9136-9140	CHEBI_EXT:33290	denotes	chow
T2395	9195-9199	CHEBI_EXT:33290	denotes	chow
T2396	9223-9233	CHEBI_EXT:33282	denotes	antibiotic
T2397	9254-9265	GO:0007631	denotes	consumption
T2398	9277-9282	NCBITaxon:10088	denotes	mouse
T2399	9318-9324	NCBITaxon:33208	denotes	animal
T2400	9334-9337	CHEBI:50845	denotes	dox
T2401	9364-9370	NCBITaxon:33208	denotes	animal
T2402	9399-9402	CHEBI:50845	denotes	dox
T2403	9412-9416	UBERON_EXT:body	denotes	body
T2404	9433-9437	CHEBI_EXT:33290	denotes	Chow
T2405	9480-9492	_FRAGMENT	denotes	breakdown of
T2406	9497-9507	GO:0017001	denotes	antibiotic
T2407	9497-9507	CHEBI_EXT:33282	denotes	antibiotic
T2579	9571-9580	SO_EXT:0000902	denotes	transgene
T2580	9588-9604	_FRAGMENT	denotes	amplification of
T2581	9613-9616	GO:0006277	denotes	DNA
T2582	9605-9616	SO_EXT:genomic_DNA	denotes	genomic DNA
T2583	9613-9616	CHEBI_SO_EXT:DNA	denotes	DNA
T2584	9639-9643	UBERON:0002415	denotes	tail
T2585	9652-9657	UBERON:0002415	denotes	Tails
T2586	9709-9713	CHEBI:32145	denotes	NaOH
T2587	9772-9776	CHEBI:9754	denotes	Tris
T2588	9777-9780	CHEBI:17883	denotes	HCl
T2589	9899-9902	PR_EXT:000004168	denotes	APP
T2590	9911-9921	SO_EXT:0000902	denotes	transgenes
T2591	9982-9989	SO_EXT:0000112	denotes	primers
T2592	9991-9994	PR_EXT:000004168	denotes	APP
T2593	10015-10029	SO_EXT:0000121	denotes	forward primer
T2594	10063-10066	PR_EXT:000004168	denotes	APP
T2595	10067-10071	SO_EXT:cDNA	denotes	cDNA
T2596	10114-10128	SO_EXT:0000132	denotes	reverse primer
T2597	10129-10132	PR_EXT:000001886	denotes	PrP
T2598	10153-10159	SO_EXT:0000205	denotes	3′ UTR
T2599	10167-10173	SO_EXT:0000440	denotes	vector
T2600	10216-10225	SO_EXT:0000902	denotes	transgene
T2601	10247-10253	SO_EXT:0000112	denotes	primer
T2602	10308-10315	SO:0001030	denotes	forward
T2603	10335-10339	PR_EXT:P04483	denotes	Tn10
T2604	10340-10352	CHEBI:27902	denotes	tetracycline
T2605	10404-10411	SO:0001031	denotes	reverse
T2606	10423-10427	NCBITaxon:10298	denotes	HSV1
T2607	10428-10432	PR_EXT:P06492	denotes	VP16
T2608	10487-10496	SO_EXT:0000902	denotes	transgene
T2609	10526-10536	_FRAGMENT	denotes	segment of
T2610	10566-10570	SO_EXT:0000842	denotes	gene
T2611	10552-10565	PR_EXT:000001886	denotes	prion protein
T2612	10558-10565	CHEBI_PR_EXT:protein	denotes	protein
T2613	10588-10591	CHEBI_SO_EXT:DNA	denotes	DNA
T2614	10608-10622	SO_EXT:0000121	denotes	forward primer
T2615	10624-10627	PR_EXT:000001886	denotes	PrP
T2616	10649-10654	NCBITaxon:10088	denotes	mouse
T2617	10655-10658	PR_EXT:000001886	denotes	PrP
T2618	10659-10677	SO_EXT:0000236	denotes	open reading frame
T2619	10717-10731	SO_EXT:0000132	denotes	reverse primer
T2620	10733-10736	PR_EXT:000001886	denotes	PrP
T2621	10757-10763	SO_EXT:0000205	denotes	3′ UTR
T2622	10782-10785	PR_EXT:000001886	denotes	PrP
T2623	10786-10790	SO_EXT:0000704	denotes	gene
T2624	10799-10808	SO_EXT:0000902	denotes	transgene
T2625	10809-10815	SO_EXT:0000440	denotes	vector
T2626	10934-10944	SO_EXT:transgenic_entity	denotes	transgenic
T2627	10949-10958	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T2628	10971-10973	SO_EXT:0000028	denotes	bp
T2629	11002-11005	PR_EXT:000001886	denotes	PrP
T2630	11006-11010	SO_EXT:0000704	denotes	gene
T2631	11016-11019	PR_EXT:000004168	denotes	APP
T2632	11020-11029	SO_EXT:0000902	denotes	transgene
T2633	11064-11066	SO_EXT:0000028	denotes	bp
T2634	11107-11109	SO_EXT:0000028	denotes	bp
T3087	11147-11155	UBERON:0001851	denotes	cortical
T3088	11165-11174	UBERON:0001890	denotes	forebrain
T3089	11175-11181	UBERON:0000479	denotes	tissue
T3090	11231-11240	CHEBI_EXT:phosphate	denotes	phosphate
T3091	11241-11249	CHEBI_CHMO_EXT:buffer_process	denotes	buffered
T3092	11250-11256	CHEBI_EXT:saline_solution	denotes	saline
T3093	11273-11277	CHEBI_EXT:EDTA	denotes	EDTA
T3094	11282-11301	CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor	denotes	protease inhibitors
T3095	11303-11312	NCBITaxon:40674	denotes	Mammalian
T3096	11313-11317	CL_GO_EXT:cell	denotes	cell
T3097	11318-11326	CHEBI:60004	denotes	cocktail
T3098	11380-11385	CHEBI_SO_EXT:molecular_probe	denotes	probe
T3099	11500-11504	CHEBI_EXT:EDTA	denotes	EDTA
T3100	11505-11523	CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor	denotes	protease inhibitor
T3101	11653-11658	UBERON:0000955	denotes	brain
T3102	11720-11724	CHEBI:9754	denotes	Tris
T3103	11725-11728	CHEBI:17883	denotes	HCl
T3104	11843-11847	CHEBI:9754	denotes	Tris
T3105	11848-11855	CHEBI_SO_EXT:glycine	denotes	glycine
T3106	11856-11878	CHEBI:8984	denotes	sodium dodecyl sulfate
T3107	11885-11888	CHEBI:8984	denotes	SDS
T3108	11890-11896	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T3109	11957-11961	CHEBI:9754	denotes	Tris
T3110	11962-11969	CHEBI:46760	denotes	tricine
T3111	11970-11973	CHEBI:8984	denotes	SDS
T3112	11974-11980	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T3113	12038-12046	CHEBI_PR_EXT:protein	denotes	Proteins
T3114	12052-12063	GO_EXT:biological_movement_or_translocation_process	denotes	transferred
T3115	12094-12108	CHEBI:53325	denotes	nitrocellulose
T3116	12180-12186	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T3117	12241-12244	CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue	denotes	fat
T3118	12249-12253	UBERON:0001913	denotes	milk
T3119	12316-12324	CHEBI:75958	denotes	solution
T3120	12351-12361	GO:0042571	denotes	antibodies
T3121	12363-12368	NCBITaxon:10088	denotes	mouse
T3122	12464-12469	NCBITaxon:10088	denotes	mouse
T3123	12563-12569	NCBITaxon:9986	denotes	rabbit
T3124	12586-12596	CHEBI:18421	denotes	superoxide
T3125	12586-12606	GO_EXT:0004784	denotes	superoxide dismutase
T3126	12586-12608	PR_EXT:000015399	denotes	superoxide dismutase 1
T3127	12612-12617	PR_EXT:P00441	denotes	hSOD1
T3128	12655-12661	NCBITaxon:9986	denotes	rabbit
T3129	12784-12792	CHEBI:53424	denotes	Tween-20
T3130	12825-12829	NCBITaxon:9925	denotes	goat
T3131	12835-12840	NCBITaxon:10088	denotes	mouse
T3132	12845-12849	NCBITaxon:9925	denotes	goat
T3133	12855-12861	NCBITaxon:9986	denotes	rabbit
T3134	12866-12876	MOP:0000779	denotes	conjugated
T3135	12887-12895	GO:0042571	denotes	antibody
T3136	12924-12932	CHEBI:75958	denotes	solution
T3137	12983-12991	CHEBI:53424	denotes	Tween-20
T3138	13046-13053	CHEBI_EXT:33893	denotes	reagent
T3139	13082-13091	CHEBI_EXT:polyatomic_entity_or_group	denotes	Molecular
T3140	13209-13218	CHEBI_EXT:polyatomic_entity_or_group	denotes	Molecular
T3679	13478-13482	CHEBI_SO_EXT:mRNA	denotes	mRNA
T3680	13528-13531	CHEBI_SO_EXT:RNA	denotes	RNA
T3681	13560-13565	UBERON:0000955	denotes	brain
T3682	13567-13572	UBERON:0002107	denotes	liver
T3683	13574-13580	UBERON:0002113	denotes	kidney
T3684	13582-13587	UBERON:0000948	denotes	heart
T3685	13589-13593	UBERON:0002048	denotes	lung
T3686	13595-13601	UBERON:0002106	denotes	spleen
T3687	13607-13622	UBERON_EXT:skeletal_muscle_organ_or_tissue	denotes	skeletal muscle
T3688	13668-13682	CHEBI:53325	denotes	nitrocellulose
T3689	13735-13738	CHEBI_EXT:saline_sodium_citrate	denotes	SSC
T3690	13760-13766	SO_EXT:sequence_fusion_process	denotes	linked
T3691	13760-13766	MOP:0000779	denotes	linked
T3692	13785-13797	CHEBI_EXT:radiolabel_process	denotes	radiolabeled
T3693	13803-13805	SO_EXT:0000028	denotes	bp
T3694	13817-13821	SO_EXT:cDNA	denotes	cDNA
T3695	13834-13836	NCBITaxon:10088	denotes	mo
T3696	13846-13850	SO_EXT:cDNA	denotes	cDNA
T3697	13858-13869	GO:0097617	denotes	hybridizing
T3698	13904-13908	CHEBI_EXT:EDTA	denotes	EDTA
T3699	13915-13931	CHEBI:37586	denotes	sodium phosphate
T3700	13932-13938	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T3701	13951-13954	CHEBI:8984	denotes	SDS
T3702	14031-14035	CHEBI_EXT:EDTA	denotes	EDTA
T3703	14042-14058	CHEBI:37586	denotes	sodium phosphate
T3704	14059-14065	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T3705	14078-14081	CHEBI:8984	denotes	SDS
T3706	14132-14136	CHEBI_EXT:EDTA	denotes	EDTA
T3707	14143-14159	CHEBI:37586	denotes	sodium phosphate
T3708	14160-14166	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T3709	14179-14182	CHEBI:8984	denotes	SDS
T4051	14270-14277	CHEBI:60425	denotes	Amyloid
T4052	14288-14292	NCBITaxon:10088	denotes	Mice
T4053	14312-14317	CHEBI:25698	denotes	ether
T4054	14333-14339	UBERON:0000955	denotes	brains
T4055	14377-14393	CHEBI_EXT:paraformaldehye	denotes	paraformaldehyde
T4056	14416-14424	CHEBI_EXT:50913	denotes	fixative
T4057	14434-14440	UBERON:0000955	denotes	brains
T4058	14446-14457	GO_EXT:biological_movement_or_translocation_process	denotes	transferred
T4059	14480-14488	CHEBI:30879	denotes	alcohols
T4060	14521-14537	CHEBI:31832	denotes	methylsalicylate
T4061	14588-14594	CHEBI_EXT:silver	denotes	silver
T4062	14601-14607	CHEBI_EXT:silver	denotes	Silver
T4063	14785-14791	CHEBI:27338	denotes	xylene
T4064	14796-14804	CHEBI:30879	denotes	alcohols
T4065	14814-14819	CHEBI:15377	denotes	water
T4066	14855-14869	CHEBI:32130	denotes	silver nitrate
T4067	14870-14878	CHEBI:75958	denotes	solution
T4068	14936-14941	CHEBI:15377	denotes	water
T4069	14971-14985	CHEBI:32130	denotes	silver nitrate
T4070	14986-14994	CHEBI:75958	denotes	solution
T4071	15015-15033	CHEBI:18219	denotes	ammonium hydroxide
T4072	15073-15086	CHEBI:18219	denotes	ammonia water
T4073	15162-15174	CHEBI:16842	denotes	formaldehyde
T4074	15196-15201	CHEBI:15377	denotes	water
T4075	15225-15236	CHEBI:48107	denotes	nitric acid
T4076	15251-15262	CHEBI:30769	denotes	citric acid
T4077	15291-15305	CHEBI:32130	denotes	silver nitrate
T4078	15306-15314	CHEBI:75958	denotes	solution
T4079	15347-15352	CHEBI:15377	denotes	water
T4080	15409-15417	CHEBI:30879	denotes	alcohols
T4081	15422-15428	CHEBI:27338	denotes	xylene
T4082	15501-15507	CHEBI:27338	denotes	xylene
T4083	15512-15520	CHEBI:30879	denotes	alcohols
T4084	15522-15529	CHEBI:60425	denotes	amyloid
T4085	15709-15714	CHEBI:15377	denotes	water
T4086	15774-15782	CHEBI:75958	denotes	solution
T4087	15837-15848	CHEBI:16995	denotes	oxalic acid
T4088	15849-15857	CHEBI:75958	denotes	solution
T4089	15918-15925	CHEBI:15377	denotes	aqueous
T4090	15945-15953	CHEBI:75958	denotes	solution
T4091	16063-16070	CHEBI:16236	denotes	ethanol
T4092	16094-16099	CHEBI:15377	denotes	water
T4093	16137-16144	CHEBI:15377	denotes	aqueous
T4094	16196-16205	PR_EXT:ubiquitin	denotes	Ubiquitin
T4095	16207-16212	CL:0000125	denotes	glial
T4096	16207-16238	PR_EXT:000007939	denotes	glial fibrillary acidic protein
T4097	16224-16230	CHEBI_EXT:37527	denotes	acidic
T4098	16231-16238	CHEBI_PR_EXT:protein	denotes	protein
T4099	16244-16246	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T4100	16361-16367	CHEBI:27338	denotes	xylene
T4101	16402-16410	CHEBI:30879	denotes	alcohols
T4102	16420-16425	CHEBI:15377	denotes	water
T4103	16438-16448	GO_EXT:0004601	denotes	peroxidase
T4104	16493-16510	CHEBI:16240	denotes	hydrogen peroxide
T4105	16514-16522	CHEBI:17790	denotes	methanol
T4106	16562-16567	CHEBI:15377	denotes	water
T4107	16663-16667	NCBITaxon:9925	denotes	goat
T4108	16668-16673	UBERON:0001977	denotes	serum
T4109	16683-16695	CHEBI:9750	denotes	Triton-X 100
T4110	16741-16749	GO:0042571	denotes	antibody
T4111	16751-16757	NCBITaxon:9986	denotes	rabbit
T4112	16763-16765	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T4113	16766-16773	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T4114	16785-16793	GO:0042571	denotes	antibody
T4115	16863-16869	NCBITaxon:9986	denotes	rabbit
T4116	16875-16884	PR_EXT:ubiquitin	denotes	ubiquitin
T4117	16889-16895	NCBITaxon:9986	denotes	rabbit
T4118	16901-16906	CL:0000125	denotes	glial
T4119	16901-16932	PR_EXT:000007939	denotes	glial fibrillary acidic protein
T4120	16918-16924	CHEBI_EXT:37527	denotes	acidic
T4121	16925-16932	CHEBI_PR_EXT:protein	denotes	protein
T4122	16934-16938	PR_EXT:000007939	denotes	GFAP
T4123	16951-16961	GO:0042571	denotes	antibodies
T4124	17045-17049	NCBITaxon:9925	denotes	goat
T4125	17050-17055	UBERON:0001977	denotes	serum
T4126	17138-17146	GO:0042571	denotes	antibody
T4127	17236-17242	NCBITaxon:9986	denotes	rabbit
T4128	17266-17268	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T4129	17333-17343	GO_EXT:0004601	denotes	peroxidase
T4130	17349-17359	GO_EXT:0004601	denotes	peroxidase
T4131	17360-17368	CHEBI_EXT:33893	denotes	reagents
T4132	17375-17385	PR_EXT:ubiquitin	denotes	ubitquitin
T4133	17395-17399	PR_EXT:000007939	denotes	GFAP
T4134	17508-17516	GO:0042571	denotes	Antibody
T4135	17517-17524	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T4136	17601-17612	CHEBI:51686	denotes	hematoxylin
T4769	17652-17660	UBERON:0001851	denotes	cortical
T4770	17749-17752	CHEBI:8984	denotes	SDS
T4771	17831-17834	CHEBI:8984	denotes	SDS
T4772	18082-18084	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T4773	18085-18093	GO:0042571	denotes	antibody
T4774	18143-18151	CHEBI:75958	denotes	solution
T4775	18168-18171	CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue	denotes	fat
T4776	18176-18180	UBERON:0001913	denotes	milk
T4777	18256-18264	CHEBI:53424	denotes	Tween-20
T4778	18310-18320	MOP:0000779	denotes	conjugated
T4779	18321-18328	CHEBI_PR_EXT:protein	denotes	protein
T4780	18321-18330	PR_EXT:000029158	denotes	protein A
T4781	18367-18375	CHEBI:75958	denotes	solution
T4782	18438-18446	CHEBI:53424	denotes	Tween-20
T4783	18455-18463	GO:0042571	denotes	antibody
T4784	18464-18471	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T4785	18619-18628	CHEBI_EXT:polyatomic_entity_or_group	denotes	Molecular
T4786	18686-18688	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T5049	18758-18760	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T5050	18781-18789	UBERON:0001851	denotes	cortical
T5051	18914-18917	CHEBI:8984	denotes	SDS
T5052	18927-18938	CHEBI:30751	denotes	formic acid
T5053	18940-18942	CHEBI:30751	denotes	FA
T5054	18999-19005	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T5055	19224-19232	CHEBI:75958	denotes	solution
T5056	19253-19256	CHEBI:8984	denotes	SDS
T5057	19285-19291	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T5058	19301-19303	CHEBI:30751	denotes	FA
T5059	19333-19337	CHEBI:9754	denotes	Tris
T5060	19338-19347	CHEBI_EXT:phosphate	denotes	phosphate
T5061	19348-19354	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T5062	19384-19390	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T5063	19409-19414	NCBITaxon:9606	denotes	Human
T5064	19415-19417	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T5065	19473-19480	CHEBI_SO_EXT:epitope	denotes	epitope
T5066	19522-19526	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T5067	19531-19535	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T5068	19559-19567	CHEBI_EXT:chemical_substance_or_polyatomic_entity	denotes	Chemical
T5069	19601-19603	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T5070	19605-19610	NCBITaxon:10088	denotes	mouse
T5071	19613-19618	NCBITaxon:9606	denotes	human
T5072	19702-19709	CHEBI_SO_EXT:epitope	denotes	epitope
T5073	19832-19840	UBERON:0001851	denotes	cortical
T5074	19841-19847	UBERON:0000479	denotes	tissue
T5330	19909-19916	PR_EXT:000003199	denotes	CaMKIIα
T5331	19947-19951	NCBITaxon:10088	denotes	mice
T5332	19979-19986	NCBITaxon:33208	denotes	Animals
T5333	20085-20093	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T5334	20113-20121	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T5335	20122-20125	CHEBI:50845	denotes	dox
T5336	20224-20230	NCBITaxon:33208	denotes	animal
T5337	20231-20237	GO_EXT:biological_movement_or_translocation_process	denotes	passed
T5338	20570-20576	NCBITaxon:33208	denotes	animal
T5508	20685-20692	CHEBI_PR_EXT:protein	denotes	protein
T5509	20693-20703	GO:0010467	denotes	expression
T6117	21102-21111	SO_EXT:0000902	denotes	transgene
T6118	21122-21129	GO:0010467	denotes	express
T6119	21139-21145	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6120	21166-21168	NCBITaxon:10088	denotes	mo
T6121	21219-21231	CHEBI:27902	denotes	tetracycline
T6122	21243-21251	SO_EXT:0000167	denotes	promoter
T6123	21261-21271	SO_EXT:transgenic_entity	denotes	Transgenic
T6124	21272-21275	PR_EXT:000004168	denotes	APP
T6125	21276-21286	GO:0010467	denotes	expression
T6126	21328-21332	NCBITaxon:10088	denotes	mice
T6127	21336-21343	NCBITaxon:33208	denotes	animals
T6128	21364-21371	GO:0065007	denotes	control
T6129	21379-21386	PR_EXT:000003199	denotes	CaMKIIα
T6130	21387-21395	SO_EXT:0000167	denotes	promoter
T6131	21482-21486	NCBITaxon:10088	denotes	mice
T6132	21521-21530	SO_EXT:0000902	denotes	transgene
T6133	21554-21564	GO:0010467	denotes	expressing
T6134	21619-21622	PR_EXT:000004168	denotes	APP
T6135	21623-21633	SO_EXT:transgenic_entity	denotes	transgenic
T6136	21657-21664	CHEBI:60425	denotes	amyloid
T6137	21770-21780	SO_EXT:transgenic_entity	denotes	transgenic
T6138	21781-21784	PR_EXT:000004168	denotes	APP
T6139	21785-21792	CHEBI_PR_EXT:protein	denotes	protein
T6140	21899-21902	PR_EXT:000004168	denotes	APP
T6141	21916-21926	SO_EXT:transgenic_entity	denotes	transgenic
T6142	21939-21942	PR_EXT:000004168	denotes	APP
T6143	21943-21947	NCBITaxon:10088	denotes	mice
T6144	21957-21965	GO:0042571	denotes	antibody
T6145	21998-22001	PR_EXT:000004168	denotes	APP
T6146	22007-22014	CHEBI_PR_EXT:amyloid_beta	denotes	amyloid
T6147	22007-22039	PR_EXT:000004137	denotes	amyloid precursor-like protein 2
T6148	22030-22037	CHEBI_PR_EXT:protein	denotes	protein
T6149	22049-22059	SO_EXT:transgenic_entity	denotes	transgenic
T6150	22060-22067	CHEBI_PR_EXT:protein	denotes	protein
T6151	22080-22088	GO:0042571	denotes	antibody
T6152	23797-23800	PR_EXT:000004168	denotes	APP
T6153	23801-23805	NCBITaxon:10088	denotes	mice
T6154	23848-23857	SO_EXT:0000902	denotes	transgene
T6155	23868-23871	CHEBI:50845	denotes	dox
T6156	24016-24025	SO_EXT:0000902	denotes	transgene
T6157	24087-24090	CHEBI:50845	denotes	dox
T6158	24096-24099	CHEBI:50845	denotes	dox
T6159	24167-24171	NCBITaxon:10088	denotes	mice
T6160	24176-24180	GO:0007567	denotes	born
T6161	24195-24198	CHEBI:50845	denotes	dox
T6162	24232-24235	CHEBI:50845	denotes	dox
T6163	24282-24285	CHEBI:50845	denotes	dox
T6164	24324-24328	CHEBI_EXT:33290	denotes	chow
T6165	24372-24379	NCBITaxon:33208	denotes	Animals
T6166	24380-24384	GO:0007567	denotes	born
T6167	24399-24402	CHEBI:50845	denotes	dox
T6168	24415-24425	SO_EXT:transgenic_entity	denotes	transgenic
T6169	24426-24429	PR_EXT:000004168	denotes	APP
T6170	24474-24477	CHEBI:50845	denotes	dox
T6171	24489-24499	SO_EXT:transgenic_entity	denotes	transgenic
T6172	24500-24503	PR_EXT:000004168	denotes	APP
T6173	24504-24514	GO:0010467	denotes	expression
T6174	24560-24563	CHEBI:50845	denotes	dox
T6175	24585-24595	GO:0010467	denotes	expression
T6176	24625-24629	NCBITaxon:10088	denotes	mice
T6177	24661-24671	SO_EXT:transgenic_entity	denotes	transgenic
T6178	24672-24679	CHEBI_PR_EXT:protein	denotes	protein
T6179	24707-24710	CHEBI:50845	denotes	dox
T6180	24779-24792	GO_EXT:transcription	denotes	transcription
T6181	24844-24847	PR_EXT:000004168	denotes	APP
T6182	24865-24875	SO_EXT:transgenic_entity	denotes	transgenic
T6183	24889-24894	PR_EXT:APLP	denotes	APLPs
T6184	24933-24940	NCBITaxon:33208	denotes	animals
T6185	24993-25003	SO_EXT:transgenic_entity	denotes	transgenic
T6186	25004-25008	NCBITaxon:10088	denotes	mice
T6187	25145-25147	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6188	25145-25158	GO:0034205	denotes	Aβ production
T6189	25194-25197	CHEBI:50845	denotes	dox
T6190	25259-25263	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T6191	25268-25272	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6192	25292-25301	UBERON:0001890	denotes	forebrain
T6193	25333-25340	NCBITaxon:33208	denotes	animals
T6194	25362-25366	NCBITaxon:10088	denotes	mice
T6195	25382-25389	CHEBI:60425	denotes	amyloid
T6196	25447-25454	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6197	25472-25477	UBERON:0000955	denotes	brain
T6198	25488-25497	SO_EXT:0000902	denotes	transgene
T6199	25625-25632	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6200	25625-25642	GO:0043043	denotes	peptide synthesis
T6201	25705-25708	CHEBI:8984	denotes	SDS
T6202	25718-25720	CHEBI:30751	denotes	FA
T6203	25741-25749	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptides
T6204	25791-25796	NCBITaxon:9606	denotes	human
T6205	25797-25806	SO_EXT:0000902	denotes	transgene
T6206	25815-25819	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T6207	25824-25828	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6208	25842-25846	NCBITaxon:10088	denotes	mice
T6209	25871-25878	NCBITaxon:33208	denotes	animals
T6210	25900-25904	GO:0007567	denotes	born
T6211	25919-25922	CHEBI:50845	denotes	dox
T6212	25983-25986	CHEBI:50845	denotes	dox
T6213	25987-25991	CHEBI_EXT:33290	denotes	chow
T6214	26224-26233	SO_EXT:0000902	denotes	transgene
T6215	26242-26244	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6216	26282-26289	NCBITaxon:33208	denotes	animals
T6217	26290-26294	GO:0007567	denotes	born
T6218	26309-26312	CHEBI:50845	denotes	dox
T6219	26362-26372	CHEBI_EXT:33282	denotes	antibiotic
T6220	26429-26433	NCBITaxon:10088	denotes	mice
T6221	26447-26451	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6222	26487-26490	CHEBI:50845	denotes	dox
T6223	26561-26564	CHEBI:8984	denotes	SDS
T6224	26573-26577	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6225	26634-26637	CHEBI:50845	denotes	dox
T6226	26670-26672	CHEBI:30751	denotes	FA
T6227	26699-26702	CHEBI:50845	denotes	dox
T6228	26721-26728	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6229	26748-26755	NCBITaxon:33208	denotes	animals
T6230	26903-26910	NCBITaxon:33208	denotes	animals
T6231	26921-26925	GO:0007567	denotes	born
T6232	26940-26943	CHEBI:50845	denotes	dox
T6233	26997-27004	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6234	27022-27026	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6235	27051-27055	NCBITaxon:10088	denotes	mice
T6236	27078-27080	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6237	27104-27108	NCBITaxon:10088	denotes	mice
T6238	27135-27144	SO_EXT:0000902	denotes	transgene
T6239	27153-27160	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6240	27180-27182	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6241	27201-27204	PR_EXT:000004168	denotes	APP
T6242	27205-27209	NCBITaxon:10088	denotes	mice
T6243	27250-27257	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6244	27270-27280	SO_EXT:transgenic_entity	denotes	transgenic
T6245	27281-27288	NCBITaxon:33208	denotes	animals
T6246	27411-27414	CHEBI:50845	denotes	dox
T6247	27439-27449	SO_EXT:transgenic_entity	denotes	transgenic
T6248	27502-27504	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6249	30385-30394	SO_EXT:sequence_alteration_entity_or_process	denotes	mutations
T6250	30417-30421	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6251	30491-30498	NCBITaxon:33208	denotes	animals
T6252	30540-30550	SO_EXT:transgenic_entity	denotes	transgenic
T6253	30552-30559	PR_EXT:000003199	denotes	CaMKIIα
T6254	30582-30586	NCBITaxon:10088	denotes	mice
T6255	30608-30615	CHEBI:60425	denotes	amyloid
T6256	30649-30656	CHEBI:60425	denotes	Amyloid
T6257	30678-30682	NCBITaxon:10088	denotes	mice
T6258	30753-30762	UBERON:0001890	denotes	forebrain
T6259	30778-30784	UBERON:0001851	denotes	cortex
T6260	30786-30797	UBERON_EXT:hippocampus_proper_or_hippocampal_formation	denotes	hippocampus
T6261	30799-30808	GO:0007608	denotes	olfactory
T6262	30799-30813	UBERON:0002264	denotes	olfactory bulb
T6263	30819-30827	UBERON:0002435	denotes	striatum
T6264	30839-30846	PR_EXT:000003199	denotes	CaMKIIα
T6265	30847-30855	SO_EXT:0000167	denotes	promoter
T6266	30920-30927	CHEBI:60425	denotes	amyloid
T6267	30978-30984	UBERON:0001851	denotes	cortex
T6268	30989-31000	UBERON_EXT:hippocampus_proper_or_hippocampal_formation	denotes	hippocampus
T6269	31042-31052	UBERON:0002037	denotes	cerebellum
T6270	31056-31066	UBERON:0002298	denotes	brain stem
T6271	31102-31109	PR_EXT:000003199	denotes	CaMKIIα
T6272	31110-31120	GO:0065007	denotes	controlled
T6273	31121-31130	SO_EXT:0000902	denotes	transgene
T6274	31131-31141	GO:0010467	denotes	expression
T6275	31182-31187	NCBITaxon:9606	denotes	human
T6276	31238-31241	PR_EXT:000004168	denotes	APP
T6277	31242-31246	NCBITaxon:10088	denotes	mice
T6278	31353-31356	PR_EXT:000004168	denotes	APP
T6279	31357-31367	SO_EXT:transgenic_entity	denotes	transgenic
T6280	31368-31372	NCBITaxon:10088	denotes	mice
T6281	31397-31405	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T6282	31458-31461	PR_EXT:000004168	denotes	APP
T6283	31462-31466	NCBITaxon:10088	denotes	mice
T6284	31539-31546	CHEBI:60425	denotes	amyloid
T6285	31576-31584	UBERON:0001897	denotes	thalamus
T6286	31618-31622	NCBITaxon:10088	denotes	mice
T6287	31623-31633	GO:0010467	denotes	expressing
T6288	31634-31640	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6289	31641-31644	PR_EXT:000004168	denotes	APP
T6290	31653-31658	PR_EXT:000001843	denotes	Thy-1
T6291	31659-31667	SO_EXT:0000167	denotes	promoter
T6292	31685-31692	CHEBI:60425	denotes	amyloid
T6293	31741-31747	GO:0030424	denotes	axonal
T6294	31741-31757	GO:0008088	denotes	axonal transport
T6295	31761-31764	PR_EXT:000004168	denotes	APP
T6296	31765-31767	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6297	31775-31787	_FRAGMENT	denotes	terminals of
T6298	31797-31804	GO_UBERON_EXT:nerve_ending_or_neuron_projection_terminus	denotes	neurons
T6299	31788-31796	UBERON:0001851	denotes	cortical
T6300	31788-31804	CL:0010012	denotes	cortical neurons
T6301	31812-31820	UBERON:0001897	denotes	thalamus
T6302	31857-31867	SO_EXT:transgenic_entity	denotes	transgenic
T6303	31868-31872	NCBITaxon:10088	denotes	mice
T6304	31874-31884	GO:0010467	denotes	expressing
T6305	31902-31905	PR_EXT:000004168	denotes	APP
T6306	31906-31916	SO_EXT:0000902	denotes	transgenes
T6307	31928-31935	CHEBI:60425	denotes	amyloid
T6308	31952-31962	SO_EXT:transgenic_entity	denotes	transgenic
T6309	31963-31967	NCBITaxon:10088	denotes	mice
T6310	32039-32046	CHEBI:60425	denotes	amyloid
T6311	32095-32105	SO_EXT:transgenic_entity	denotes	transgenic
T6312	32106-32113	NCBITaxon:33208	denotes	animals
T6313	32114-32118	GO:0007567	denotes	born
T6314	32133-32136	CHEBI:50845	denotes	dox
T6315	32138-32145	NCBITaxon:33208	denotes	Animals
T6316	32163-32173	GO:0010467	denotes	expressing
T6317	32204-32207	CHEBI:50845	denotes	dox
T6318	32234-32241	CHEBI:60425	denotes	amyloid
T6319	32306-32315	SO_EXT:0000902	denotes	transgene
T6320	32316-32326	GO:0010467	denotes	expression
T6321	32346-32349	CHEBI:50845	denotes	dox
T6322	32378-32380	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6323	32381-32388	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6324	32399-32406	CHEBI:60425	denotes	amyloid
T6325	32480-32490	CHEBI_EXT:35222	denotes	inhibitors
T6326	32494-32496	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6327	32494-32507	GO:0034205	denotes	Aβ production
T6328	32540-32550	SO_EXT:transgenic_entity	denotes	transgenic
T6329	32551-32555	NCBITaxon:10088	denotes	mice
T6330	32557-32564	PR_EXT:000003199	denotes	CaMKIIα
T6331	32571-32574	PR_EXT:000004168	denotes	APP
T6332	32595-32599	CHEBI_EXT:33290	denotes	food
T6333	32632-32639	CHEBI:60425	denotes	amyloid
T6334	32683-32688	UBERON:0000955	denotes	brain
T6335	32711-32718	NCBITaxon:33208	denotes	animals
T6336	32745-32749	CHEBI_EXT:33290	denotes	chow
T6337	32753-32757	CHEBI_EXT:33290	denotes	food
T6338	32769-32772	CHEBI:50845	denotes	dox
T6339	32802-32812	GO_EXT:killing	denotes	sacrificed
T6340	32857-32864	NCBITaxon:33208	denotes	animals
T6341	32887-32891	CHEBI_EXT:33290	denotes	chow
T6342	33077-33087	SO_EXT:transgenic_entity	denotes	transgenic
T6343	33113-33116	CHEBI:50845	denotes	dox
T6344	33125-33132	NCBITaxon:33208	denotes	animals
T6345	33192-33201	SO_EXT:0000902	denotes	transgene
T6346	33245-33249	NCBITaxon:10088	denotes	mice
T6347	33306-33313	NCBITaxon:33208	denotes	animals
T6348	33356-33363	NCBITaxon:33208	denotes	animals
T6349	33369-33372	CHEBI:50845	denotes	dox
T6350	33433-33436	PR_EXT:000004168	denotes	APP
T6351	33437-33447	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminal
T6352	33576-33585	SO_EXT:sequence_alteration_process	denotes	processed
T6353	33586-33596	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminal
T6354	35387-35393	UBERON:0000479	denotes	Tissue
T6355	35413-35419	NCBITaxon:33208	denotes	animal
T6356	35466-35473	CHEBI:60425	denotes	amyloid
T6357	35494-35500	CHEBI_EXT:silver	denotes	silver
T6358	35519-35525	CHEBI_EXT:silver	denotes	silver
T6359	35545-35547	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6360	35628-35635	CHEBI:60425	denotes	amyloid
T6361	35700-35707	CHEBI:60425	denotes	amyloid
T6362	35743-35750	CHEBI:60425	denotes	amyloid
T6363	35764-35768	NCBITaxon:10088	denotes	mice
T6364	36033-36040	NCBITaxon:33208	denotes	animals
T6365	36055-36064	SO_EXT:0000902	denotes	transgene
T6366	36185-36192	CHEBI:60425	denotes	amyloid
T6367	36282-36288	CHEBI_EXT:silver	denotes	silver
T6368	36329-36336	CHEBI:60425	denotes	amyloid
T6369	36444-36447	CHEBI:50845	denotes	dox
T6370	36471-36481	SO_EXT:transgenic_entity	denotes	transgenic
T6371	36482-36485	PR_EXT:000004168	denotes	APP
T6372	38308-38312	NCBITaxon:10088	denotes	mice
T6373	38330-38333	CHEBI:50845	denotes	dox
T6374	38385-38388	PR_EXT:000004168	denotes	APP
T6375	38389-38393	NCBITaxon:10088	denotes	mice
T6376	38395-38402	PR_EXT:000003199	denotes	CaMKIIα
T6377	38459-38462	CHEBI:50845	denotes	dox
T6378	38503-38507	NCBITaxon:10088	denotes	mice
T6379	38537-38546	SO_EXT:0000902	denotes	transgene
T6380	38654-38657	PR_EXT:000004168	denotes	APP
T6381	38673-38682	SO_EXT:0000902	denotes	transgene
T6382	38710-38717	NCBITaxon:33208	denotes	animals
T6383	38750-38754	NCBITaxon:10088	denotes	mice
T6384	38772-38779	CHEBI:60425	denotes	amyloid
T6385	38827-38831	NCBITaxon:10088	denotes	mice
T6386	38874-38883	SO_EXT:0000902	denotes	transgene
T6387	38884-38894	GO:0010467	denotes	expression
T6388	38997-39003	CHEBI_EXT:silver	denotes	silver
T6389	39075-39078	CHEBI:50845	denotes	dox
T6390	39087-39094	NCBITaxon:33208	denotes	animals
T6391	39099-39112	CHEBI_EXT:biochemical	denotes	biochemically
T6392	39147-39149	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6393	39157-39163	UBERON:0000955	denotes	brains
T6394	39171-39175	NCBITaxon:10088	denotes	mice
T6395	39193-39202	SO_EXT:0000902	denotes	transgene
T6396	39245-39253	UBERON:0001851	denotes	cortical
T6397	39254-39260	UBERON:0000479	denotes	tissue
T6398	39271-39277	NCBITaxon:33208	denotes	animal
T6399	39314-39321	CHEBI_PR_EXT:protein	denotes	protein
T6400	39337-39343	GO_EXT:biological_movement_or_translocation_process	denotes	passed
T6401	39522-39528	GO:0007601	denotes	visual
T6402	39581-39584	PR_EXT:000004168	denotes	APP
T6403	39585-39589	NCBITaxon:10088	denotes	mice
T6405	39655-39657	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6406	39756-39760	NCBITaxon:10088	denotes	mice
T6407	39797-39799	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6408	39869-39872	PR_EXT:000004168	denotes	APP
T6409	39873-39877	NCBITaxon:10088	denotes	mice
T6410	39930-39932	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6411	39955-39962	NCBITaxon:33208	denotes	animals
T6412	40016-40025	SO_EXT:0000902	denotes	transgene
T6413	40096-40098	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6414	40106-40112	UBERON:0000955	denotes	brains
T6415	40190-40197	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6416	40224-40234	GO:0010467	denotes	expression
T6417	40251-40259	UBERON:0001851	denotes	Cortical
T6418	40312-40319	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6419	40331-40334	CHEBI:8984	denotes	SDS
T6420	40341-40343	CHEBI:30751	denotes	FA
T6421	40368-40377	SO_EXT:0000902	denotes	transgene
T6422	40386-40390	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T6423	40395-40399	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6424	40417-40422	NCBITaxon:9606	denotes	human
T6425	40451-40458	NCBITaxon:33208	denotes	animals
T6426	40469-40476	CHEBI:60425	denotes	amyloid
T6427	40522-40524	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6428	40555-40558	CHEBI:8984	denotes	SDS
T6429	40563-40565	CHEBI:30751	denotes	FA
T6430	40694-40697	CHEBI:8984	denotes	SDS
T6431	40702-40704	CHEBI:30751	denotes	FA
T6432	40713-40715	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6433	40826-40832	UBERON:0000955	denotes	brains
T6434	40929-40933	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T6435	40956-40960	NCBITaxon:10088	denotes	mice
T6436	40984-40988	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6437	41088-41092	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T6438	41097-41101	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T6439	41301-41308	NCBITaxon:33208	denotes	animals
T6440	41353-41355	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6441	41365-41372	NCBITaxon:33208	denotes	animals
T6442	43545-43553	GO:0043005	denotes	neuritic
T6443	43558-43563	CL:0000125	denotes	glial
T6444	43679-43688	SO_EXT:0000902	denotes	transgene
T6445	43714-43720	CHEBI_EXT:silver	denotes	silver
T6446	43731-43740	PR_EXT:ubiquitin	denotes	ubiquitin
T6447	43763-43771	GO:0043005	denotes	neuritic
T6448	43829-43849	CL:0002627	denotes	activated astrocytes
T6449	43868-43872	PR_EXT:000007939	denotes	GFAP
T6450	43904-43911	NCBITaxon:33208	denotes	animals
T6451	43924-43932	GO:0043005	denotes	Neuritic
T6452	43937-43942	CL:0000125	denotes	glial
T6453	43993-43997	NCBITaxon:10088	denotes	mice
T6454	44012-44021	SO_EXT:0000902	denotes	transgene
T6455	44048-44054	GO_EXT:biological_growth_entity_or_process	denotes	growth
T6456	44100-44104	NCBITaxon:10088	denotes	mice
T6457	44837-44839	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T6458	44851-44860	GO:0050890	denotes	cognitive
T6459	44882-44886	NCBITaxon:10088	denotes	mice
T6460	44913-44922	GO:0050890	denotes	cognition
T6461	44996-45005	GO:0050890	denotes	cognitive
T6462	45006-45014	GO_PATO_EXT:biological_behavior	denotes	behavior
T6463	45076-45086	SO_EXT:transgenic_entity	denotes	transgenic
T6464	45087-45091	NCBITaxon:10088	denotes	mice
T6465	45101-45104	PR_EXT:000004168	denotes	APP
T6466	45105-45112	NCBITaxon:33208	denotes	animals
T6467	45199-45207	GO:0036268	denotes	swimming
T6468	45235-45239	NCBITaxon:10088	denotes	mice
T6469	45266-45271	CHEBI:15377	denotes	water
T6470	45292-45297	CHEBI:15377	denotes	water
T6471	45315-45319	GO:0036268	denotes	swim
T6472	45446-45453	NCBITaxon:33208	denotes	animals
T6473	45524-45528	NCBITaxon:10088	denotes	mice
T6474	45666-45673	NCBITaxon:33208	denotes	animals
T6475	45732-45736	NCBITaxon:10088	denotes	mice
T6476	45788-45795	GO:0010467	denotes	express
T6477	45812-45822	SO_EXT:transgenic_entity	denotes	transgenic
T6478	45823-45830	CHEBI_PR_EXT:protein	denotes	protein
T6479	45938-45945	CHEBI:60425	denotes	amyloid
T6480	45955-45964	GO:0050890	denotes	cognitive
T6481	46064-46067	PR_EXT:000004168	denotes	APP
T6482	46068-46072	NCBITaxon:10088	denotes	mice
T6483	46122-46131	GO:0050890	denotes	cognitive
T6484	46148-46155	NCBITaxon:33208	denotes	animals
T6485	46205-46214	GO:0050890	denotes	cognitive
T6486	46306-46309	PR_EXT:000004168	denotes	APP
T6487	46310-46314	NCBITaxon:10088	denotes	mice
T6488	46362-46372	SO_EXT:transgenic_entity	denotes	transgenic
T6489	46373-46380	NCBITaxon:33208	denotes	animals
T6490	46405-46415	SO_EXT:transgenic_entity	denotes	transgenic
T10200	48181-48186	NCBITaxon:10088	denotes	mouse
T10201	48257-48259	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10202	48257-48270	GO:0034205	denotes	Aβ production
T10203	48290-48297	CHEBI:60425	denotes	amyloid
T10204	48322-48332	SO_EXT:transgenic_entity	denotes	transgenic
T10205	48333-48337	NCBITaxon:10088	denotes	mice
T10206	48373-48380	GO:0010467	denotes	express
T10207	48417-48424	GO:0065007	denotes	control
T10208	48430-48442	CHEBI:27902	denotes	tetracycline
T10209	48454-48462	SO_EXT:0000167	denotes	promoter
T10210	48499-48502	CHEBI:50845	denotes	dox
T10211	48561-48571	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminal
T10212	48603-48609	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T10213	48610-48618	CHEBI_PR_EXT:protein	denotes	proteins
T10214	48662-48672	SO_EXT:transgenic_entity	denotes	Transgenic
T10215	48673-48683	GO:0010467	denotes	expression
T10216	48731-48735	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T10217	48754-48761	CHEBI:60425	denotes	amyloid
T10218	48786-48790	NCBITaxon:10088	denotes	mice
T10219	48842-48849	CHEBI:60425	denotes	Amyloid
T10220	48906-48917	UBERON_EXT:hippocampus_proper_or_hippocampal_formation	denotes	hippocampus
T10221	48922-48928	UBERON:0001851	denotes	cortex
T10222	48942-48946	NCBITaxon:10088	denotes	mice
T10223	48982-48989	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T10224	49019-49029	SO_EXT:transgenic_entity	denotes	transgenic
T10225	49030-49033	PR_EXT:000004168	denotes	APP
T10226	49085-49092	CHEBI:60425	denotes	amyloid
T10227	49140-49147	NCBITaxon:33208	denotes	animals
T10228	49179-49186	CHEBI:60425	denotes	amyloid
T10229	49285-49295	CHEBI_EXT:35222	denotes	inhibitors
T10230	49369-49376	CHEBI:60425	denotes	amyloid
T10231	49403-49412	SO_EXT:0000902	denotes	transgene
T10232	49542-49549	CHEBI_PR_EXT:protein	denotes	protein
T10233	49569-49575	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T10234	49576-49586	PR_EXT:000008840	denotes	huntingtin
T10235	49646-49659	GO:0005576	denotes	extracellular
T10236	49651-49659	CL_GO_EXT:cell	denotes	cellular
T10237	49660-49667	CHEBI:60425	denotes	amyloid
T10238	49697-49711	CHEBI_EXT:52217	denotes	pharmaceutical
T10239	49712-49723	GO:0070765	denotes	γ-secretase
T10240	49724-49734	CHEBI_EXT:35222	denotes	inhibitors
T10241	49770-49797	GO:1902003	denotes	regulation of Aβ production
T10242	49784-49786	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10243	49875-49884	CHEBI:36357	denotes	compounds
T10244	49924-49934	SO_EXT:transgenic_entity	denotes	transgenic
T10245	49935-49939	NCBITaxon:10088	denotes	mice
T10246	49977-49981	CHEBI_PR_EXT:amyloid_beta_40	denotes	Aβ40
T10247	50035-50039	CHEBI_PR_EXT:amyloid_beta_42	denotes	Aβ42
T10248	50117-50120	PR_EXT:000004168	denotes	APP
T10249	50121-50131	GO:0010467	denotes	expression
T10250	50146-50150	NCBITaxon:10088	denotes	mice
T10251	50237-50239	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10252	50237-50250	GO:0034205	denotes	Aβ production
T10253	50317-50328	GO:0070765	denotes	γ-secretase
T10254	50329-50339	CHEBI_EXT:35222	denotes	inhibitors
T10255	50377-50398	CHEBI_EXT:52217	denotes	pharmaceutical agents
T10256	50436-50460	GO:1902003	denotes	control of Aβ production
T10257	50447-50449	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10258	50550-50552	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10259	50562-50571	CHEBI:36357	denotes	compounds
T10260	50601-50608	CHEBI:60425	denotes	amyloid
T10261	50687-50694	CHEBI:60425	denotes	amyloid
T10262	50738-50747	SO_EXT:0000902	denotes	transgene
T10263	50791-50798	NCBITaxon:33208	denotes	animals
T10264	50814-50817	CHEBI:50845	denotes	dox
T10265	50871-50878	CHEBI:60425	denotes	amyloid
T10266	51016-51023	NCBITaxon:33208	denotes	animals
T10267	51037-51040	CHEBI:50845	denotes	dox
T10268	51088-51090	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10269	51177-51186	GO_EXT:biological_release	denotes	releasing
T10270	51187-51194	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T10271	51199-51209	GO_EXT:oligomeric_macromolecular_complex	denotes	oligomeric
T10272	51210-51212	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10273	51286-51288	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10274	51286-51299	GO:0034205	denotes	Aβ production
T10275	51363-51370	CHEBI:60425	denotes	amyloid
T10276	51414-51423	UBERON:0000104	denotes	life span
T10277	51493-51499	NCBITaxon:9606	denotes	humans
T10278	51568-51577	UBERON:0000104	denotes	life span
T10279	51581-51587	NCBITaxon:9989	denotes	rodent
T10280	51789-51805	GO:0034205	denotes	production of Aβ
T10281	51803-51805	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10282	51861-51864	PR_EXT:000004168	denotes	APP
T10283	51865-51875	GO:0010467	denotes	expression
T10284	51974-51981	CHEBI:60425	denotes	amyloid
T10285	52079-52083	NCBITaxon:10088	denotes	mice
T10286	52096-52099	PR_EXT:000004168	denotes	APP
T10287	52100-52111	SO_EXT:transgenic_entity	denotes	transgenics
T10288	52134-52142	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T10289	52286-52291	NCBITaxon:9606	denotes	human
T10290	52374-52378	NCBITaxon:10088	denotes	mice
T10291	52529-52538	SO_EXT:0000902	denotes	transgene
T10292	52539-52549	GO:0010467	denotes	expression
T10293	52579-52582	CHEBI:50845	denotes	dox
T10294	52603-52608	NCBITaxon:10088	denotes	mouse
T10295	52609-52611	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10296	52696-52701	NCBITaxon:10088	denotes	mouse
T10297	52702-52704	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10298	52723-52728	NCBITaxon:9606	denotes	human
T10299	59186-59189	PR_EXT:000010173	denotes	tau
T10300	52729-52736	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T10301	52812-52819	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T10303	52875-52882	PR_EXT:000003199	denotes	CaMKIIα
T10304	52903-52907	NCBITaxon:10088	denotes	mice
T10305	52918-52921	CHEBI:50845	denotes	dox
T10306	52954-52961	CHEBI:60425	denotes	amyloid
T10307	53018-53025	SO_EXT:0000704	denotes	genetic
T10308	53053-53069	GO:0034205	denotes	production of Aβ
T10309	53067-53069	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10310	53093-53099	NCBITaxon:9606	denotes	humans
T10311	53247-53250	PR_EXT:000004168	denotes	APP
T10312	53247-53250	_FRAGMENT	denotes	APP
T10313	53254-53263	GO:0042983	denotes	synthetic
T10314	53251-53253	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10315	53251-53263	GO:0034205	denotes	Aβ synthetic
T10316	53373-53375	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10317	53409-53416	CHEBI:60425	denotes	amyloid
T10318	53466-53473	CHEBI:60425	denotes	amyloid
T10319	53486-53491	NCBITaxon:9606	denotes	human
T10320	53492-53497	UBERON:0000955	denotes	brain
T10321	53555-53560	NCBITaxon:10088	denotes	mouse
T10322	53561-53566	UBERON:0000955	denotes	brain
T10323	53581-53586	NCBITaxon:9606	denotes	human
T10324	53587-53592	UBERON:0000955	denotes	brain
T10325	53639-53643	NCBITaxon:10088	denotes	mice
T10326	53699-53706	CHEBI:60425	denotes	amyloid
T10327	53741-53748	CHEBI:60425	denotes	amyloid
T10328	53788-53793	NCBITaxon:9606	denotes	human
T10329	53814-53819	NCBITaxon:10088	denotes	mouse
T10330	53838-53848	CL:0000129	denotes	microglial
T10331	53849-53861	GO:0006909	denotes	phagocytosis
T10332	53872-53881	CL:0000129	denotes	microglia
T10333	53885-53895	SO_EXT:transgenic_entity	denotes	transgenic
T10334	53896-53901	NCBITaxon:10088	denotes	mouse
T10335	53909-53917	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localize
T10336	53921-53927	UBERON:0000479	denotes	tissue
T10337	53976-53983	CHEBI:60425	denotes	amyloid
T10338	54017-54026	CL:0000129	denotes	microglia
T10339	54039-54046	CHEBI:60425	denotes	amyloid
T10340	54058-54063	NCBITaxon:9606	denotes	human
T10341	54064-54069	UBERON:0000955	denotes	brain
T10342	54122-54132	GO:0010467	denotes	expression
T10343	54136-54155	GO_EXT:0004875	denotes	complement receptor
T10344	54180-54189	CL:0000129	denotes	microglia
T10345	54193-54200	CHEBI:60425	denotes	amyloid
T10346	54201-54211	GO:0008152	denotes	metabolism
T10347	54224-54234	SO_EXT:transgenic_entity	denotes	transgenic
T10348	54258-54263	NCBITaxon:9606	denotes	human
T10349	54355-54378	CHEBI_EXT:35472	denotes	anti-inflammatory drugs
T10350	54360-54372	GO_PATO_EXT:inflammatory_process_or_quality	denotes	inflammatory
T10351	54389-54399	CL:0000129	denotes	microglial
T10352	54389-54410	GO:0001774	denotes	microglial activation
T10353	54427-54434	CHEBI:60425	denotes	amyloid
T10354	54443-54446	PR_EXT:000004168	denotes	APP
T10355	54447-54457	SO_EXT:transgenic_entity	denotes	transgenic
T10356	54458-54462	NCBITaxon:10088	denotes	mice
T10357	54486-54491	NCBITaxon:10088	denotes	mouse
T10358	54492-54501	CL:0000129	denotes	microglia
T10359	54538-54545	CHEBI:60425	denotes	amyloid
T10360	54645-54668	CHEBI_EXT:35472	denotes	anti-inflammatory drugs
T10361	54650-54662	GO_PATO_EXT:inflammatory_process_or_quality	denotes	inflammatory
T10362	54672-54683	GO:0070765	denotes	γ-secretase
T10363	54684-54692	MOP:0000780	denotes	cleavage
T10364	54727-54736	CL:0000129	denotes	microglia
T10365	54749-54754	NCBITaxon:9606	denotes	human
T10366	54773-54778	NCBITaxon:10088	denotes	mouse
T10367	54827-54837	CL:0000129	denotes	microglial
T10368	54900-54907	CHEBI:60425	denotes	amyloid
T10369	54925-54931	UBERON:0000955	denotes	brains
T10370	54983-54986	PR_EXT:000004168	denotes	APP
T10371	54987-54991	NCBITaxon:10088	denotes	mice
T10372	55035-55044	CL:0000129	denotes	microglia
T10373	55054-55059	NCBITaxon:10088	denotes	mouse
T10374	55115-55120	CL_GO_EXT:cell	denotes	cells
T10375	55149-55156	CHEBI:60425	denotes	amyloid
T10376	55182-55185	PR_EXT:000004168	denotes	APP
T10377	55186-55190	NCBITaxon:10088	denotes	mice
T10378	55244-55247	CHEBI:50845	denotes	dox
T10379	55299-55308	CL:0000129	denotes	microglia
T10380	55324-55328	NCBITaxon:10088	denotes	mice
T10381	55330-55333	CHEBI:50845	denotes	Dox
T10382	55361-55372	CHEBI:50694	denotes	minocycline
T10383	55385-55407	CHEBI_EXT:35472	denotes	anti-inflammatory drug
T10384	55390-55402	GO_PATO_EXT:inflammatory_process_or_quality	denotes	inflammatory
T10385	55412-55421	CHEBI_EXT:35222	denotes	inhibitor
T10386	55425-55435	CL:0000129	denotes	microglial
T10387	55425-55446	GO:0001774	denotes	microglial activation
T10388	55465-55468	CHEBI:50845	denotes	dox
T10389	55484-55496	GO_PATO_EXT:inflammatory_process_or_quality	denotes	inflammatory
T10390	55550-55569	CHEBI_EXT:67079	denotes	anti-inflammatories
T10392	55622-55625	CHEBI:50845	denotes	dox
T10393	55634-55641	NCBITaxon:33208	denotes	animals
T10394	55701-55704	CHEBI:50845	denotes	dox
T10395	55736-55743	CHEBI:60425	denotes	amyloid
T10396	55799-55809	CL:0000129	denotes	microglial
T10397	55810-55819	GO_EXT:reaction_or_response	denotes	responses
T10398	55845-55850	NCBITaxon:10088	denotes	mouse
T10399	55899-55902	CHEBI:50845	denotes	dox
T10400	55912-55922	CL:0000129	denotes	microglial
T10401	56002-56009	CHEBI:60425	denotes	amyloid
T10402	56074-56076	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10403	56109-56114	NCBITaxon:10088	denotes	mouse
T10404	56156-56158	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10405	56159-56169	GO:0042571	denotes	antibodies
T10406	56197-56202	UBERON:0000955	denotes	brain
T10407	56232-56239	CHEBI:60425	denotes	amyloid
T10408	56306-56314	GO:0042571	denotes	antibody
T10409	56335-56342	CHEBI:60425	denotes	amyloid
T10410	56457-56464	CHEBI:60425	denotes	amyloid
T10411	56566-56576	NCBITaxon:11646	denotes	lentiviral
T10412	56577-56585	GO_EXT:biological_movement_or_translocation_process	denotes	transfer
T10413	56589-56599	PR_EXT:000001898	denotes	neprilysin
T10414	56734-56742	GO:0042571	denotes	antibody
T10415	56781-56800	CL:0002629	denotes	activated microglia
T10416	56923-56931	SO_EXT:sequence_deletion_entity_or_process	denotes	deletion
T10417	56939-56950	GO_EXT:0019763	denotes	Fc receptor
T10418	56964-56972	GO_EXT:0004872	denotes	receptor
T10419	56977-56987	CL:0000129	denotes	microglial
T10420	57004-57012	GO:0042571	denotes	antibody
T10421	57013-57020	CHEBI_EXT:59132	denotes	antigen
T10422	57021-57030	GO:0032991	denotes	complexes
T10423	57035-57038	PR_EXT:000004168	denotes	APP
T10424	57039-57049	SO_EXT:transgenic_entity	denotes	transgenic
T10425	57050-57055	NCBITaxon:10088	denotes	mouse
T10426	57101-57109	GO:0042571	denotes	antibody
T10427	57179-57188	CL:0000129	denotes	microglia
T10428	57179-57199	GO:0001774	denotes	microglia activation
T10429	57296-57309	UBERON:0002405	denotes	immune system
T10430	57378-57386	GO:0042571	denotes	antibody
T10431	57390-57395	NCBITaxon:10239	denotes	viral
T10432	57426-57449	GO:0001774	denotes	activation of microglia
T10433	57440-57449	CL:0000129	denotes	microglia
T10434	57528-57540	GO:0006909	denotes	phagocytosis
T10435	57568-57570	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10436	57595-57605	PR_EXT:000001898	denotes	neprilysin
T10437	57609-57611	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10438	57621-57631	GO:0042571	denotes	antibodies
T10439	57670-57677	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T10440	57728-57751	GO:0001774	denotes	activation of microglia
T10441	57742-57751	CL:0000129	denotes	microglia
T10442	57760-57770	SO_EXT:transgenic_entity	denotes	transgenic
T10443	57771-57781	GO:0010467	denotes	expression
T10444	57785-57789	PR_EXT:000000046	denotes	TGFβ
T10445	57819-57837	CHEBI:16412	denotes	lipopolysaccharide
T10446	57898-57901	PR_EXT:000004168	denotes	APP
T10447	57902-57912	SO_EXT:transgenic_entity	denotes	transgenic
T10448	57913-57917	NCBITaxon:10088	denotes	mice
T10449	57944-57952	GO:0042571	denotes	antibody
T10450	57998-58010	GO_PATO_EXT:inflammatory_process_or_quality	denotes	inflammation
T10451	58031-58039	GO:0042571	denotes	antibody
T10452	58044-58049	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bound
T10453	58050-58057	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T10454	58104-58106	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10455	58132-58139	CHEBI:60425	denotes	amyloid
T10456	58244-58253	CL:0000129	denotes	microglia
T10457	58257-58262	NCBITaxon:10088	denotes	mouse
T10458	58343-58349	NCBITaxon:9606	denotes	humans
T10459	58398-58402	NCBITaxon:10088	denotes	mice
T10460	58408-58415	GO:0010467	denotes	express
T10461	58422-58427	NCBITaxon:9606	denotes	human
T10462	58428-58431	PR_EXT:000010173	denotes	tau
T10463	58446-58452	SO_EXT:0000440	denotes	vector
T10464	58484-58487	PR_EXT:000004168	denotes	APP
T10465	58488-58492	NCBITaxon:10088	denotes	mice
T10466	58522-58525	PR_EXT:000010173	denotes	tau
T10467	58522-58549	GO:0097418	denotes	tau neurofibrillary tangles
T10468	58556-58563	CHEBI:60425	denotes	amyloid
T10469	58611-58620	SO_EXT:0000902	denotes	transgene
T10470	58737-58744	CHEBI_PR_EXT:protein	denotes	protein
T10471	58781-58786	NCBITaxon:10088	denotes	mouse
T10472	58907-58910	CHEBI:50845	denotes	dox
T10473	58920-58929	SO_EXT:0000902	denotes	transgene
T10474	58979-58986	CHEBI_PR_EXT:protein	denotes	protein
T10475	59064-59071	CHEBI_PR_EXT:protein	denotes	protein
T10476	59179-59181	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10477	59235-59242	CHEBI_PR_EXT:protein	denotes	protein
T10478	59275-59281	_FRAGMENT	denotes	intra-
T10479	59291-59299	GO:0005622	denotes	cellular
T10480	59286-59299	GO:0005576	denotes	extracellular
T10481	59291-59299	CL_GO_EXT:cell	denotes	cellular
T10482	59399-59411	_FRAGMENT	denotes	synthesis of
T10483	59416-59418	GO:0034205	denotes	Aβ
T10484	59416-59418	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10485	59444-59453	GO:0050890	denotes	cognitive
T10486	59488-59491	PR_EXT:000010173	denotes	tau
T10487	59492-59496	NCBITaxon:10088	denotes	mice
T10488	59510-59517	CHEBI_PR_EXT:protein	denotes	protein
T10489	59560-59569	GO:0050890	denotes	cognitive
T10490	59592-59601	SO_EXT:0000902	denotes	transgene
T10491	59657-59666	GO:0050890	denotes	cognitive
T10492	59667-59677	GO_PATO_EXT:biological_behavior	denotes	behavioral
T10493	59729-59732	PR_EXT:000004168	denotes	APP
T10494	59733-59737	NCBITaxon:10088	denotes	mice
T10495	59797-59804	CHEBI:60425	denotes	amyloid
T10496	59834-59837	PR_EXT:000004168	denotes	APP
T10497	59838-59842	NCBITaxon:10088	denotes	mice
T10498	59987-59997	SO_EXT:transgenic_entity	denotes	transgenic
T10499	59998-60002	NCBITaxon:10088	denotes	mice
T10500	60039-60047	GO:0036268	denotes	swimming
T10501	60100-60105	CHEBI:15377	denotes	water
T10502	60165-60173	GO:0007612	denotes	learning
T10503	60178-60184	GO:0007613	denotes	memory
T10504	60266-60274	GO_EXT:biological_movement_or_translocation_process	denotes	movement
T10505	60303-60312	GO:0050890	denotes	cognitive
T10506	60313-60323	GO_PATO_EXT:biological_behavior	denotes	behavioral
T10507	60411-60421	GO:0010467	denotes	expression
T10508	60459-60470	GO:0006508	denotes	proteolytic
T10509	60560-60563	CHEBI:50845	denotes	dox
T10510	60571-60575	NCBITaxon:10088	denotes	mice
T10511	60726-60736	SO_EXT:transgenic_entity	denotes	transgenic
T10512	60737-60740	PR_EXT:000004168	denotes	APP
T10513	60737-60750	GO:0042983	denotes	APP synthesis
T10514	60852-60860	CL:0000540	denotes	neuronal
T10515	60880-60889	SO_EXT:0000902	denotes	transgene
T10516	60890-60900	GO:0010467	denotes	expression
T10517	60918-60923	GO:0007567	denotes	natal
T10518	61055-61057	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10519	61055-61068	GO:0034205	denotes	Aβ production
T10520	61118-61128	SO_EXT:transgenic_entity	denotes	transgenic
T10521	61129-61134	NCBITaxon:10088	denotes	mouse
T10522	61212-61214	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10523	61212-61224	GO:0034205	denotes	Aβ synthesis
T10524	61226-61234	GO:0043005	denotes	neuritic
T10525	61363-61365	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10526	61363-61376	GO:0034205	denotes	Aβ production
T10527	61387-61389	CHEBI_PR_EXT:amyloid_beta	denotes	Aβ
T10528	61415-61427	GO:0006909	denotes	phagocytosis
T10529	61441-61448	CHEBI:60425	denotes	amyloid
T10530	61572-61582	CHEBI_EXT:35222	denotes	inhibitors
T10531	61665-61672	CHEBI:60425	denotes	amyloid
T10532	61750-61760	CL:0000129	denotes	microglial
T10533	61750-61771	GO:0001774	denotes	microglial activation
T10534	61775-61780	NCBITaxon:9606	denotes	human
T10535	61839-61846	CHEBI:60425	denotes	amyloid
T10536	61874-61886	GO:0006909	denotes	phagocytosis
T10537	61977-61987	CHEBI_EXT:35222	denotes	inhibitors
T10538	62041-62046	NCBITaxon:9606	denotes	human
T10539	62047-62052	UBERON:0000955	denotes	brain
T10540	62080-62087	CHEBI:60425	denotes	amyloid
T2141	8457-8467	GO:0010467	denotes	expressing
T10302	52853-52860	CHEBI:60425	denotes	amyloid
T10391	55607-55614	CHEBI:60425	denotes	amyloid
T6404	39603-39606	CHEBI:50845	denotes	dox
T6491	46423-46433	SO_EXT:transgenic_entity	denotes	transgenic
T6492	46486-46496	GO:0040011	denotes	ambulation
T6493	46555-46565	SO_EXT:transgenic_entity	denotes	transgenic
T6494	46566-46570	NCBITaxon:10088	denotes	mice
T6495	46734-46747	GO:0007623	denotes	diurnal cycle
T6496	46847-46850	PR_EXT:000004168	denotes	APP
T6497	46851-46855	NCBITaxon:10088	denotes	mice
T6498	46914-46922	GO_PATO_EXT:biological_behavior	denotes	behavior
T6499	46980-46989	SO_EXT:0000902	denotes	transgene
T6500	47114-47117	PR_EXT:000004168	denotes	APP
T6501	47118-47122	NCBITaxon:10088	denotes	mice
T6502	47126-47129	CHEBI:50845	denotes	dox
T6503	47131-47138	NCBITaxon:33208	denotes	Animals
T6504	47139-47143	GO:0007567	denotes	born
T6505	47158-47161	CHEBI:50845	denotes	dox
T6506	47257-47260	CHEBI:50845	denotes	dox
T6507	47268-47275	NCBITaxon:33208	denotes	animals
T6508	47282-47292	SO_EXT:transgenic_entity	denotes	transgenic
T6509	47297-47306	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T6510	47323-47340	GO:0007623	denotes	circadian rhythms
R1621	T2406	T2405	_lexicallyChainedTo	antibiotic,breakdown of
R1	T83	T82	_lexicallyChainedTo	peptide,Aβ
R1716	T2581	T2580	_lexicallyChainedTo	DNA,amplification of
R1717	T2610	T2609	_lexicallyChainedTo	gene,segment of
R3966	T6298	T6297	_lexicallyChainedTo	neurons,terminals of
R6983	T10313	T10312	_lexicallyChainedTo	synthetic,APP
R6984	T10479	T10478	_lexicallyChainedTo	cellular,intra-
R6985	T10483	T10482	_lexicallyChainedTo	Aβ,synthesis of
R321	T624	T623	_lexicallyChainedTo	2,presenilins
R322	T661	T660	_lexicallyChainedTo	termini,N-

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T36	48-61	GO:0034205	denotes	Aβ Production
T37	197-203	MOP:0000780	denotes	cleave
T38	236-261	PR:000004168	denotes	amyloid precursor protein
T39	263-266	PR:000004168	denotes	APP
T40	412-425	GO:0034205	denotes	Aβ production
T41	433-438	UBERON:0000955	denotes	brain
T42	565-571	UBERON:0000479	denotes	tissue
T43	587-594	CHEBI:60425	denotes	amyloid
T44	640-646	NCBITaxon:33208	denotes	animal
T45	657-664	CHEBI:60425	denotes	amyloid
T46	777-782	NCBITaxon:10088	denotes	mouse
T47	794-805	SO:0000704	denotes	genetically
T48	827-840	GO:0034205	denotes	Aβ production
T49	880-890	CHEBI:35222	denotes	inhibitors
T50	898-902	NCBITaxon:10088	denotes	mice
T51	907-914	GO:0010467	denotes	express
T52	922-925	PR:000004168	denotes	APP
T53	933-939	SO:0000440	denotes	vector
T54	952-961	GO:0065007	denotes	regulated
T55	965-976	CHEBI:50845	denotes	doxycycline
T56	1014-1024	GO:0010467	denotes	expression
T57	1028-1031	PR:000004168	denotes	APP
T58	1058-1065	CHEBI:60425	denotes	amyloid
T59	1090-1101	CHEBI:50845	denotes	doxycycline
T60	1137-1140	PR:000004168	denotes	APP
T61	1141-1151	GO:0010467	denotes	expression
T62	1184-1197	GO:0034205	denotes	Aβ production
T63	1231-1235	NCBITaxon:10088	denotes	mice
T64	1263-1275	GO:0034205	denotes	Aβ synthesis
T65	1324-1331	CHEBI:60425	denotes	amyloid
T66	1384-1391	CHEBI:60425	denotes	amyloid
T67	1496-1500	NCBITaxon:10088	denotes	Mice
T68	1510-1513	PR:000004168	denotes	APP
T69	1510-1523	GO:0042983	denotes	APP synthesis
T70	1616-1623	CHEBI:60425	denotes	amyloid
T71	1709-1716	CHEBI:60425	denotes	amyloid
T72	1739-1743	NCBITaxon:10088	denotes	mice
T73	1848-1861	GO:0034205	denotes	Aβ production
T74	2002-2009	CHEBI:60425	denotes	amyloid
T549	2286-2293	CHEBI:60425	denotes	amyloid
T550	2515-2522	SO:0000704	denotes	genetic
T551	2574-2599	PR:000004168	denotes	amyloid precursor protein
T552	2601-2604	PR:000004168	denotes	APP
T553	2637-2650	PR:000013344	denotes	presenilins 1
T554	2637-2648	_FRAGMENT	denotes	presenilins
T555	2655-2656	PR:000013345	denotes	2
T556	2713-2720	GO:0032991	denotes	complex
T557	2743-2746	PR:000004168	denotes	APP
T558	2793-2809	GO:0034205	denotes	production of Aβ
T559	3024-3032	GO:0042571	denotes	antibody
T560	3103-3109	NCBITaxon:9606	denotes	humans
T561	3156-3163	CHEBI:60425	denotes	amyloid
T562	3346-3353	CHEBI:60425	denotes	amyloid
T563	3388-3397	GO:0050890	denotes	cognitive
T564	3473-3481	GO:0042571	denotes	antibody
T565	3544-3554	GO:0042571	denotes	antibodies
T566	3604-3609	UBERON:0000955	denotes	brain
T567	3839-3842	PR:000004168	denotes	APP
T568	3872-3875	PR:000004168	denotes	APP
T569	3910-3933	PR:000004615	denotes	β-APP cleaving enzyme 1
T570	3916-3924	MOP:0000780	denotes	cleaving
T571	3935-3940	PR:000004615	denotes	BACE1
T572	3946-3957	GO:0070765	denotes	γ-secretase
T573	3965-3971	MOP:0000780	denotes	cleave
T574	3976-3987	CHEBI:33837	denotes	holoprotein
T575	4041-4051	CHEBI:35222	denotes	inhibitors
T576	4055-4066	GO:0070765	denotes	γ-secretase
T577	4111-4119	CHEBI:36357	denotes	molecule
T578	4120-4130	CHEBI:35222	denotes	inhibitors
T579	4134-4157	PR:000004615	denotes	β-APP cleaving enzyme 1
T580	4140-4148	MOP:0000780	denotes	cleaving
T581	4252-4258	NCBITaxon:9606	denotes	humans
T582	4326-4349	PR:000004615	denotes	β-APP cleaving enzyme 1
T583	4332-4340	MOP:0000780	denotes	cleaving
T584	4412-4417	NCBITaxon:10088	denotes	mouse
T585	4516-4521	UBERON:0000955	denotes	brain
T586	4545-4552	CHEBI:60425	denotes	amyloid
T587	4567-4583	GO:0034205	denotes	production of Aβ
T588	4618-4625	CHEBI:60425	denotes	amyloid
T589	4677-4683	NCBITaxon:33208	denotes	animal
T590	4704-4712	CHEBI:33893	denotes	reagents
T591	4769-4779	CHEBI:35222	denotes	inhibitors
T592	4801-4806	NCBITaxon:9606	denotes	human
T593	4866-4871	NCBITaxon:10088	denotes	mouse
T594	4896-4903	CHEBI:60425	denotes	amyloid
T595	4909-4918	GO:0010467	denotes	expresses
T596	4921-4933	GO:0065007	denotes	controllable
T597	4934-4937	PR:000004168	denotes	APP
T598	4938-4947	SO:0000902	denotes	transgene
T599	5007-5016	GO:0065007	denotes	regulated
T600	5031-5043	CHEBI:27902	denotes	tetracycline
T601	5060-5064	CHEBI:33290	denotes	food
T602	5068-5073	CHEBI:15377	denotes	water
T603	5094-5104	GO:0010467	denotes	expression
T604	5138-5145	SO:0000440	denotes	vectors
T605	5188-5198	GO:0010467	denotes	expression
T606	5235-5247	CHEBI:27902	denotes	tetracycline
T607	5309-5323	CHEBI:52217	denotes	pharmaceutical
T608	5324-5333	CHEBI:35222	denotes	inhibitor
T609	5337-5350	GO:0034205	denotes	Aβ production
T610	5383-5390	CHEBI:60425	denotes	amyloid
T1361	5493-5502	SO:0000902	denotes	Transgene
T1362	5529-5541	CHEBI:27902	denotes	tetracycline
T1363	5562-5567	NCBITaxon:10088	denotes	mouse
T1364	5568-5573	NCBITaxon:9606	denotes	human
T1365	5606-5612	SO:0000440	denotes	vector
T1366	5630-5645	SO:0000167	denotes	promoter region
T1367	5653-5658	PR:P04925	denotes	moPrP
T1368	5664-5670	SO:0000440	denotes	vector
T1369	5717-5729	CHEBI:27902	denotes	tetracycline
T1370	5741-5749	SO:0000167	denotes	promoter
T1371	5830-5838	GO:0006266	denotes	ligating
T1372	5839-5844	NCBITaxon:10088	denotes	mouse
T1373	5845-5848	PR:000004168	denotes	APP
T1374	5869-5875	SO:0000417	denotes	domain
T1375	5877-5879	NCBITaxon:10088	denotes	mo
T1376	5908-5914	SO:0000440	denotes	vector
T1377	5940-5952	CHEBI:27902	denotes	tetracycline
T1378	5964-5972	SO:0000167	denotes	promoter
T1379	5974-5976	SO:0000028	denotes	bp
T1380	6013-6020	SO:0000112	denotes	primers
T1381	6041-6046	PR:P23940	denotes	BamHI
T1382	6082-6087	PR:P23940	denotes	BamHI
T1383	6134-6139	PR:P23940	denotes	BamHI
T1384	6157-6165	SO:0000167	denotes	promoter
T1385	6167-6174	SO:0001030	denotes	forward
T1386	6231-6238	SO:0001031	denotes	reverse
T1387	6307-6312	PR:P04925	denotes	moPrP
T1388	6318-6324	SO:0000188	denotes	intron
T1389	6347-6354	SO:0000112	denotes	primers
T1390	6378-6383	PR:P23940	denotes	BamHI
T1391	6406-6410	SO:0000147	denotes	exon
T1392	6448-6452	SO:0000147	denotes	exon
T1393	6456-6463	SO:0001030	denotes	forward
T1394	6505-6512	SO:0001031	denotes	reverse
T1395	6562-6568	SO:0000188	denotes	intron
T1396	6607-6613	SO:0000440	denotes	vector
T1397	6702-6709	GO:0006266	denotes	ligated
T1398	6743-6748	PR:P04925	denotes	moPrP
T1399	6765-6769	SO:0000147	denotes	exon
T1400	6773-6777	SO:0000147	denotes	exon
T1401	6785-6791	SO:0000205	denotes	3′ UTR
T1402	6837-6843	SO:0000440	denotes	vector
T1403	6859-6864	SO:0000147	denotes	exons
T1404	6879-6885	SO:0000188	denotes	intron
T1405	6893-6901	SO:0000167	denotes	promoter
T1406	6908-6914	SO:0000440	denotes	vector
T1407	6938-6943	PR:P23940	denotes	BamHI
T1408	6967-6973	SO:0000188	denotes	intron
T1409	6982-6988	SO:0000112	denotes	primer
T1410	6994-7001	GO:0006266	denotes	ligated
T1411	7016-7021	PR:P23940	denotes	BamHI
T1412	7022-7025	MOP:0000780	denotes	cut
T1413	7026-7038	CHEBI:27902	denotes	tetracycline
T1414	7039-7047	SO:0000167	denotes	promoter
T1415	7063-7071	GO:0006266	denotes	ligation
T1416	7091-7097	SO:0000440	denotes	vector
T1417	7111-7123	CHEBI:27902	denotes	tetracycline
T1418	7124-7132	SO:0000167	denotes	promoter
T1419	7158-7163	SO:0000147	denotes	exons
T1420	7169-7175	SO:0000188	denotes	intron
T1421	7194-7200	SO:0000205	denotes	3′ UTR
T1422	7208-7213	PR:P04925	denotes	moPrP
T1423	7219-7225	SO:0000440	denotes	vector
T1424	7266-7272	SO:0000440	denotes	vector
T1425	7355-7357	NCBITaxon:10088	denotes	mo
T1426	7403-7409	SO:0000112	denotes	primer
T1427	7505-7512	SO:0000112	denotes	primers
T1428	7556-7563	SO:0001030	denotes	forward
T1429	7621-7628	SO:0001031	denotes	reverse
T1430	7669-7676	SO:0001030	denotes	forward
T1431	7714-7721	SO:0001031	denotes	reverse
T1432	7732-7744	SO:0000006	denotes	PCR products
T1433	7750-7757	GO:0006266	denotes	ligated
T1434	7822-7824	NCBITaxon:10088	denotes	mo
T1435	7840-7846	SO:0000440	denotes	vector
T1436	7919-7924	PR:P04925	denotes	moPrP
T1437	7930-7936	SO:0000440	denotes	vector
T2092	7976-7986	GO:0045120	denotes	Pronuclear
T2093	8054-8059	PR:P04925	denotes	moPrP
T2094	8065-8067	NCBITaxon:10088	denotes	mo
T2095	8084-8090	SO:0000440	denotes	vector
T2096	8178-8184	SO:0000440	denotes	vector
T2097	8207-8217	GO:0045120	denotes	pronucleus
T2098	8221-8231	GO:0009566	denotes	fertilized
T2099	8232-8236	CL:0000025	denotes	eggs
T2100	8262-8269	GO:0007618	denotes	matings
T2101	8279-8286	NCBITaxon:33208	denotes	animals
T2102	8325-8334	SO:0000902	denotes	transgene
T2103	8370-8373	PR:000001886	denotes	PrP
T2104	8376-8379	PR:000001886	denotes	PrP
T2105	8383-8390	SO:0000112	denotes	primers
T2106	8408-8417	SO:0000902	denotes	Transgene
T2107	8449-8456	NCBITaxon:33208	denotes	animals
T2108	8457-8467	GO:0010467	denotes	expressing
T2109	8472-8484	CHEBI:27902	denotes	tetracycline
T2110	8512-8519	GO:0065007	denotes	control
T2111	8527-8556	PR:000003199	denotes	calcium-calmodulin kinase IIα
T2112	8558-8565	PR:000003199	denotes	CaMKIIα
T2113	8567-8575	SO:0000167	denotes	promoter
T2114	8674-8680	PR:000003199	denotes	Camk2a
T2115	8768-8771	PR:000004168	denotes	APP
T2116	8803-8806	PR:000004168	denotes	APP
T2117	8818-8822	NCBITaxon:10088	denotes	mice
T2118	8843-8847	CHEBI:33290	denotes	food
T2119	8852-8857	CHEBI:15377	denotes	water
T2120	8870-8876	NCBITaxon:33208	denotes	Animal
T2121	8995-9001	NCBITaxon:33208	denotes	Animal
T2373	9028-9039	CHEBI:50845	denotes	Doxycycline
T2374	9055-9066	CHEBI:50845	denotes	Doxycycline
T2375	9068-9071	CHEBI:50845	denotes	dox
T2376	9121-9124	CHEBI:50845	denotes	dox
T2377	9136-9140	CHEBI:33290	denotes	chow
T2378	9195-9199	CHEBI:33290	denotes	chow
T2379	9223-9233	CHEBI:33282	denotes	antibiotic
T2380	9254-9265	GO:0007631	denotes	consumption
T2381	9277-9282	NCBITaxon:10088	denotes	mouse
T2382	9318-9324	NCBITaxon:33208	denotes	animal
T2383	9334-9337	CHEBI:50845	denotes	dox
T2384	9364-9370	NCBITaxon:33208	denotes	animal
T2385	9399-9402	CHEBI:50845	denotes	dox
T2386	9433-9437	CHEBI:33290	denotes	Chow
T2387	9480-9492	_FRAGMENT	denotes	breakdown of
T2388	9497-9507	GO:0017001	denotes	antibiotic
T2389	9497-9507	CHEBI:33282	denotes	antibiotic
T2529	9571-9580	SO:0000902	denotes	transgene
T2530	9588-9604	_FRAGMENT	denotes	amplification of
T2531	9613-9616	GO:0006277	denotes	DNA
T2532	9605-9612	SO:0001026	denotes	genomic
T2533	9639-9643	UBERON:0002415	denotes	tail
T2534	9652-9657	UBERON:0002415	denotes	Tails
T2535	9709-9713	CHEBI:32145	denotes	NaOH
T2536	9772-9776	CHEBI:9754	denotes	Tris
T2537	9777-9780	CHEBI:17883	denotes	HCl
T2538	9899-9902	PR:000004168	denotes	APP
T2539	9911-9921	SO:0000902	denotes	transgenes
T2540	9982-9989	SO:0000112	denotes	primers
T2541	9991-9994	PR:000004168	denotes	APP
T2542	10015-10029	SO:0000121	denotes	forward primer
T2543	10063-10066	PR:000004168	denotes	APP
T2544	10114-10128	SO:0000132	denotes	reverse primer
T2545	10129-10132	PR:000001886	denotes	PrP
T2546	10153-10159	SO:0000205	denotes	3′ UTR
T2547	10167-10173	SO:0000440	denotes	vector
T2548	10216-10225	SO:0000902	denotes	transgene
T2549	10247-10253	SO:0000112	denotes	primer
T2550	10308-10315	SO:0001030	denotes	forward
T2551	10335-10339	PR:P04483	denotes	Tn10
T2552	10340-10352	CHEBI:27902	denotes	tetracycline
T2553	10404-10411	SO:0001031	denotes	reverse
T2554	10423-10427	NCBITaxon:10298	denotes	HSV1
T2555	10428-10432	PR:P06492	denotes	VP16
T2556	10487-10496	SO:0000902	denotes	transgene
T2557	10526-10536	_FRAGMENT	denotes	segment of
T2558	10566-10570	SO:0000842	denotes	gene
T2559	10552-10565	PR:000001886	denotes	prion protein
T2560	10608-10622	SO:0000121	denotes	forward primer
T2561	10624-10627	PR:000001886	denotes	PrP
T2562	10649-10654	NCBITaxon:10088	denotes	mouse
T2563	10655-10658	PR:000001886	denotes	PrP
T2564	10659-10677	SO:0000236	denotes	open reading frame
T2565	10717-10731	SO:0000132	denotes	reverse primer
T2566	10733-10736	PR:000001886	denotes	PrP
T2567	10757-10763	SO:0000205	denotes	3′ UTR
T2568	10782-10785	PR:000001886	denotes	PrP
T2569	10786-10790	SO:0000704	denotes	gene
T2570	10799-10808	SO:0000902	denotes	transgene
T2571	10809-10815	SO:0000440	denotes	vector
T2572	10971-10973	SO:0000028	denotes	bp
T2573	11002-11005	PR:000001886	denotes	PrP
T2574	11006-11010	SO:0000704	denotes	gene
T2575	11016-11019	PR:000004168	denotes	APP
T2576	11020-11029	SO:0000902	denotes	transgene
T2577	11064-11066	SO:0000028	denotes	bp
T2578	11107-11109	SO:0000028	denotes	bp
T3052	11147-11155	UBERON:0001851	denotes	cortical
T3053	11165-11174	UBERON:0001890	denotes	forebrain
T3054	11175-11181	UBERON:0000479	denotes	tissue
T3055	11303-11312	NCBITaxon:40674	denotes	Mammalian
T3056	11318-11326	CHEBI:60004	denotes	cocktail
T3057	11653-11658	UBERON:0000955	denotes	brain
T3058	11720-11724	CHEBI:9754	denotes	Tris
T3059	11725-11728	CHEBI:17883	denotes	HCl
T3060	11843-11847	CHEBI:9754	denotes	Tris
T3061	11856-11878	CHEBI:8984	denotes	sodium dodecyl sulfate
T3062	11885-11888	CHEBI:8984	denotes	SDS
T3063	11957-11961	CHEBI:9754	denotes	Tris
T3064	11962-11969	CHEBI:46760	denotes	tricine
T3065	11970-11973	CHEBI:8984	denotes	SDS
T3066	12094-12108	CHEBI:53325	denotes	nitrocellulose
T3067	12249-12253	UBERON:0001913	denotes	milk
T3068	12316-12324	CHEBI:75958	denotes	solution
T3069	12351-12361	GO:0042571	denotes	antibodies
T3070	12363-12368	NCBITaxon:10088	denotes	mouse
T3071	12464-12469	NCBITaxon:10088	denotes	mouse
T3072	12563-12569	NCBITaxon:9986	denotes	rabbit
T3073	12586-12596	CHEBI:18421	denotes	superoxide
T3074	12586-12608	PR:000015399	denotes	superoxide dismutase 1
T3075	12612-12617	PR:P00441	denotes	hSOD1
T3076	12655-12661	NCBITaxon:9986	denotes	rabbit
T3077	12784-12792	CHEBI:53424	denotes	Tween-20
T3078	12825-12829	NCBITaxon:9925	denotes	goat
T3079	12835-12840	NCBITaxon:10088	denotes	mouse
T3080	12845-12849	NCBITaxon:9925	denotes	goat
T3081	12855-12861	NCBITaxon:9986	denotes	rabbit
T3082	12866-12876	MOP:0000779	denotes	conjugated
T3083	12887-12895	GO:0042571	denotes	antibody
T3084	12924-12932	CHEBI:75958	denotes	solution
T3085	12983-12991	CHEBI:53424	denotes	Tween-20
T3086	13046-13053	CHEBI:33893	denotes	reagent
T3661	13560-13565	UBERON:0000955	denotes	brain
T3662	13567-13572	UBERON:0002107	denotes	liver
T3663	13574-13580	UBERON:0002113	denotes	kidney
T3664	13582-13587	UBERON:0000948	denotes	heart
T3665	13589-13593	UBERON:0002048	denotes	lung
T3666	13595-13601	UBERON:0002106	denotes	spleen
T3667	13607-13615	UBERON:0004288	denotes	skeletal
T3668	13668-13682	CHEBI:53325	denotes	nitrocellulose
T3669	13760-13766	MOP:0000779	denotes	linked
T3670	13803-13805	SO:0000028	denotes	bp
T3671	13834-13836	NCBITaxon:10088	denotes	mo
T3672	13858-13869	GO:0097617	denotes	hybridizing
T3673	13915-13931	CHEBI:37586	denotes	sodium phosphate
T3674	13951-13954	CHEBI:8984	denotes	SDS
T3675	14042-14058	CHEBI:37586	denotes	sodium phosphate
T3676	14078-14081	CHEBI:8984	denotes	SDS
T3677	14143-14159	CHEBI:37586	denotes	sodium phosphate
T3678	14179-14182	CHEBI:8984	denotes	SDS
T3982	14270-14277	CHEBI:60425	denotes	Amyloid
T3983	14288-14292	NCBITaxon:10088	denotes	Mice
T3984	14312-14317	CHEBI:25698	denotes	ether
T3985	14333-14339	UBERON:0000955	denotes	brains
T3986	14416-14424	CHEBI:50913	denotes	fixative
T3987	14434-14440	UBERON:0000955	denotes	brains
T3988	14480-14488	CHEBI:30879	denotes	alcohols
T3989	14521-14537	CHEBI:31832	denotes	methylsalicylate
T3990	14785-14791	CHEBI:27338	denotes	xylene
T3991	14796-14804	CHEBI:30879	denotes	alcohols
T3992	14814-14819	CHEBI:15377	denotes	water
T3993	14855-14869	CHEBI:32130	denotes	silver nitrate
T3994	14870-14878	CHEBI:75958	denotes	solution
T3995	14936-14941	CHEBI:15377	denotes	water
T3996	14971-14985	CHEBI:32130	denotes	silver nitrate
T3997	14986-14994	CHEBI:75958	denotes	solution
T3998	15015-15033	CHEBI:18219	denotes	ammonium hydroxide
T3999	15073-15086	CHEBI:18219	denotes	ammonia water
T4000	15162-15174	CHEBI:16842	denotes	formaldehyde
T4001	15196-15201	CHEBI:15377	denotes	water
T4002	15225-15236	CHEBI:48107	denotes	nitric acid
T4003	15251-15262	CHEBI:30769	denotes	citric acid
T4004	15291-15305	CHEBI:32130	denotes	silver nitrate
T4005	15306-15314	CHEBI:75958	denotes	solution
T4006	15347-15352	CHEBI:15377	denotes	water
T4007	15409-15417	CHEBI:30879	denotes	alcohols
T4008	15422-15428	CHEBI:27338	denotes	xylene
T4009	15501-15507	CHEBI:27338	denotes	xylene
T4010	15512-15520	CHEBI:30879	denotes	alcohols
T4011	15522-15529	CHEBI:60425	denotes	amyloid
T4012	15709-15714	CHEBI:15377	denotes	water
T4013	15774-15782	CHEBI:75958	denotes	solution
T4014	15837-15848	CHEBI:16995	denotes	oxalic acid
T4015	15849-15857	CHEBI:75958	denotes	solution
T4016	15918-15925	CHEBI:15377	denotes	aqueous
T4017	15945-15953	CHEBI:75958	denotes	solution
T4018	16063-16070	CHEBI:16236	denotes	ethanol
T4019	16094-16099	CHEBI:15377	denotes	water
T4020	16137-16144	CHEBI:15377	denotes	aqueous
T4021	16207-16212	CL:0000125	denotes	glial
T4022	16207-16238	PR:000007939	denotes	glial fibrillary acidic protein
T4023	16224-16230	CHEBI:37527	denotes	acidic
T4024	16361-16367	CHEBI:27338	denotes	xylene
T4025	16402-16410	CHEBI:30879	denotes	alcohols
T4026	16420-16425	CHEBI:15377	denotes	water
T4027	16493-16510	CHEBI:16240	denotes	hydrogen peroxide
T4028	16514-16522	CHEBI:17790	denotes	methanol
T4029	16562-16567	CHEBI:15377	denotes	water
T4030	16663-16667	NCBITaxon:9925	denotes	goat
T4031	16668-16673	UBERON:0001977	denotes	serum
T4032	16683-16695	CHEBI:9750	denotes	Triton-X 100
T4033	16741-16749	GO:0042571	denotes	antibody
T4034	16751-16757	NCBITaxon:9986	denotes	rabbit
T4035	16785-16793	GO:0042571	denotes	antibody
T4036	16863-16869	NCBITaxon:9986	denotes	rabbit
T4037	16889-16895	NCBITaxon:9986	denotes	rabbit
T4038	16901-16906	CL:0000125	denotes	glial
T4039	16901-16932	PR:000007939	denotes	glial fibrillary acidic protein
T4040	16918-16924	CHEBI:37527	denotes	acidic
T4041	16934-16938	PR:000007939	denotes	GFAP
T4042	16951-16961	GO:0042571	denotes	antibodies
T4043	17045-17049	NCBITaxon:9925	denotes	goat
T4044	17050-17055	UBERON:0001977	denotes	serum
T4045	17138-17146	GO:0042571	denotes	antibody
T4046	17236-17242	NCBITaxon:9986	denotes	rabbit
T4047	17360-17368	CHEBI:33893	denotes	reagents
T4048	17395-17399	PR:000007939	denotes	GFAP
T4049	17508-17516	GO:0042571	denotes	Antibody
T4050	17601-17612	CHEBI:51686	denotes	hematoxylin
T4757	17652-17660	UBERON:0001851	denotes	cortical
T4758	17749-17752	CHEBI:8984	denotes	SDS
T4759	17831-17834	CHEBI:8984	denotes	SDS
T4760	18085-18093	GO:0042571	denotes	antibody
T4761	18143-18151	CHEBI:75958	denotes	solution
T4762	18176-18180	UBERON:0001913	denotes	milk
T4763	18256-18264	CHEBI:53424	denotes	Tween-20
T4764	18310-18320	MOP:0000779	denotes	conjugated
T4765	18321-18330	PR:000029158	denotes	protein A
T4766	18367-18375	CHEBI:75958	denotes	solution
T4767	18438-18446	CHEBI:53424	denotes	Tween-20
T4768	18455-18463	GO:0042571	denotes	antibody
T5036	18781-18789	UBERON:0001851	denotes	cortical
T5037	18914-18917	CHEBI:8984	denotes	SDS
T5038	18927-18938	CHEBI:30751	denotes	formic acid
T5039	18940-18942	CHEBI:30751	denotes	FA
T5040	19224-19232	CHEBI:75958	denotes	solution
T5041	19253-19256	CHEBI:8984	denotes	SDS
T5042	19301-19303	CHEBI:30751	denotes	FA
T5043	19333-19337	CHEBI:9754	denotes	Tris
T5044	19409-19414	NCBITaxon:9606	denotes	Human
T5045	19605-19610	NCBITaxon:10088	denotes	mouse
T5046	19613-19618	NCBITaxon:9606	denotes	human
T5047	19832-19840	UBERON:0001851	denotes	cortical
T5048	19841-19847	UBERON:0000479	denotes	tissue
T5324	19909-19916	PR:000003199	denotes	CaMKIIα
T5325	19947-19951	NCBITaxon:10088	denotes	mice
T5326	19979-19986	NCBITaxon:33208	denotes	Animals
T5327	20122-20125	CHEBI:50845	denotes	dox
T5328	20224-20230	NCBITaxon:33208	denotes	animal
T5329	20570-20576	NCBITaxon:33208	denotes	animal
T5507	20693-20703	GO:0010467	denotes	expression
T5826	21122-21129	GO:0010467	denotes	express
T5827	21166-21168	NCBITaxon:10088	denotes	mo
T5828	21219-21231	CHEBI:27902	denotes	tetracycline
T5829	21243-21251	SO:0000167	denotes	promoter
T5830	21272-21275	PR:000004168	denotes	APP
T5831	21276-21286	GO:0010467	denotes	expression
T5832	21328-21332	NCBITaxon:10088	denotes	mice
T5833	21336-21343	NCBITaxon:33208	denotes	animals
T5834	21364-21371	GO:0065007	denotes	control
T5835	21379-21386	PR:000003199	denotes	CaMKIIα
T5836	21387-21395	SO:0000167	denotes	promoter
T5837	21482-21486	NCBITaxon:10088	denotes	mice
T5838	21521-21530	SO:0000902	denotes	transgene
T5839	21554-21564	GO:0010467	denotes	expressing
T5840	21619-21622	PR:000004168	denotes	APP
T5841	21657-21664	CHEBI:60425	denotes	amyloid
T5842	21781-21784	PR:000004168	denotes	APP
T5843	21899-21902	PR:000004168	denotes	APP
T5844	21939-21942	PR:000004168	denotes	APP
T5845	21943-21947	NCBITaxon:10088	denotes	mice
T5846	21957-21965	GO:0042571	denotes	antibody
T5847	21998-22001	PR:000004168	denotes	APP
T5848	22007-22039	PR:000004137	denotes	amyloid precursor-like protein 2
T5849	22080-22088	GO:0042571	denotes	antibody
T5850	23797-23800	PR:000004168	denotes	APP
T5851	23801-23805	NCBITaxon:10088	denotes	mice
T5852	23848-23857	SO:0000902	denotes	transgene
T5853	23868-23871	CHEBI:50845	denotes	dox
T5854	24016-24025	SO:0000902	denotes	transgene
T5855	24087-24090	CHEBI:50845	denotes	dox
T5856	24096-24099	CHEBI:50845	denotes	dox
T5857	24167-24171	NCBITaxon:10088	denotes	mice
T5858	24176-24180	GO:0007567	denotes	born
T5859	24195-24198	CHEBI:50845	denotes	dox
T5860	24232-24235	CHEBI:50845	denotes	dox
T5861	24282-24285	CHEBI:50845	denotes	dox
T5862	24324-24328	CHEBI:33290	denotes	chow
T5863	24372-24379	NCBITaxon:33208	denotes	Animals
T5864	24380-24384	GO:0007567	denotes	born
T5865	24399-24402	CHEBI:50845	denotes	dox
T5866	24426-24429	PR:000004168	denotes	APP
T5867	24474-24477	CHEBI:50845	denotes	dox
T5868	24500-24503	PR:000004168	denotes	APP
T5869	24504-24514	GO:0010467	denotes	expression
T5870	24560-24563	CHEBI:50845	denotes	dox
T5871	24585-24595	GO:0010467	denotes	expression
T5872	24625-24629	NCBITaxon:10088	denotes	mice
T5873	24707-24710	CHEBI:50845	denotes	dox
T5874	24844-24847	PR:000004168	denotes	APP
T5875	24933-24940	NCBITaxon:33208	denotes	animals
T5876	25004-25008	NCBITaxon:10088	denotes	mice
T5877	25145-25158	GO:0034205	denotes	Aβ production
T5878	25194-25197	CHEBI:50845	denotes	dox
T5879	25292-25301	UBERON:0001890	denotes	forebrain
T5880	25333-25340	NCBITaxon:33208	denotes	animals
T5881	25362-25366	NCBITaxon:10088	denotes	mice
T5882	25382-25389	CHEBI:60425	denotes	amyloid
T5883	25472-25477	UBERON:0000955	denotes	brain
T5884	25488-25497	SO:0000902	denotes	transgene
T5885	25625-25642	GO:0043043	denotes	peptide synthesis
T5886	25705-25708	CHEBI:8984	denotes	SDS
T5887	25718-25720	CHEBI:30751	denotes	FA
T5888	25791-25796	NCBITaxon:9606	denotes	human
T5889	25797-25806	SO:0000902	denotes	transgene
T5890	25842-25846	NCBITaxon:10088	denotes	mice
T5891	25871-25878	NCBITaxon:33208	denotes	animals
T5892	25900-25904	GO:0007567	denotes	born
T5893	25919-25922	CHEBI:50845	denotes	dox
T5894	25983-25986	CHEBI:50845	denotes	dox
T5895	25987-25991	CHEBI:33290	denotes	chow
T5896	26224-26233	SO:0000902	denotes	transgene
T5897	26282-26289	NCBITaxon:33208	denotes	animals
T5898	26290-26294	GO:0007567	denotes	born
T5899	26309-26312	CHEBI:50845	denotes	dox
T5900	26362-26372	CHEBI:33282	denotes	antibiotic
T5901	26429-26433	NCBITaxon:10088	denotes	mice
T5902	26487-26490	CHEBI:50845	denotes	dox
T5903	26561-26564	CHEBI:8984	denotes	SDS
T5904	26634-26637	CHEBI:50845	denotes	dox
T5905	26670-26672	CHEBI:30751	denotes	FA
T5906	26699-26702	CHEBI:50845	denotes	dox
T5907	26748-26755	NCBITaxon:33208	denotes	animals
T5908	26903-26910	NCBITaxon:33208	denotes	animals
T5909	26921-26925	GO:0007567	denotes	born
T5910	26940-26943	CHEBI:50845	denotes	dox
T5911	27051-27055	NCBITaxon:10088	denotes	mice
T5912	27104-27108	NCBITaxon:10088	denotes	mice
T5913	27135-27144	SO:0000902	denotes	transgene
T5914	27201-27204	PR:000004168	denotes	APP
T5915	27205-27209	NCBITaxon:10088	denotes	mice
T5916	27281-27288	NCBITaxon:33208	denotes	animals
T5917	27411-27414	CHEBI:50845	denotes	dox
T5918	30491-30498	NCBITaxon:33208	denotes	animals
T5919	30552-30559	PR:000003199	denotes	CaMKIIα
T5920	30582-30586	NCBITaxon:10088	denotes	mice
T5921	30608-30615	CHEBI:60425	denotes	amyloid
T5922	30649-30656	CHEBI:60425	denotes	Amyloid
T5923	30678-30682	NCBITaxon:10088	denotes	mice
T5924	30753-30762	UBERON:0001890	denotes	forebrain
T5925	30778-30784	UBERON:0001851	denotes	cortex
T5926	30799-30808	GO:0007608	denotes	olfactory
T5927	30799-30813	UBERON:0002264	denotes	olfactory bulb
T5928	30819-30827	UBERON:0002435	denotes	striatum
T5929	30839-30846	PR:000003199	denotes	CaMKIIα
T5930	30847-30855	SO:0000167	denotes	promoter
T5931	30920-30927	CHEBI:60425	denotes	amyloid
T5932	30978-30984	UBERON:0001851	denotes	cortex
T5933	31042-31052	UBERON:0002037	denotes	cerebellum
T5934	31056-31066	UBERON:0002298	denotes	brain stem
T5935	31102-31109	PR:000003199	denotes	CaMKIIα
T5936	31110-31120	GO:0065007	denotes	controlled
T5937	31121-31130	SO:0000902	denotes	transgene
T5938	31131-31141	GO:0010467	denotes	expression
T5939	31182-31187	NCBITaxon:9606	denotes	human
T5940	31238-31241	PR:000004168	denotes	APP
T5941	31242-31246	NCBITaxon:10088	denotes	mice
T5942	31353-31356	PR:000004168	denotes	APP
T5943	31368-31372	NCBITaxon:10088	denotes	mice
T5944	31458-31461	PR:000004168	denotes	APP
T5945	31462-31466	NCBITaxon:10088	denotes	mice
T5946	31539-31546	CHEBI:60425	denotes	amyloid
T5947	31576-31584	UBERON:0001897	denotes	thalamus
T5948	31618-31622	NCBITaxon:10088	denotes	mice
T5949	31623-31633	GO:0010467	denotes	expressing
T5950	31641-31644	PR:000004168	denotes	APP
T5951	31653-31658	PR:000001843	denotes	Thy-1
T5952	31659-31667	SO:0000167	denotes	promoter
T5953	31685-31692	CHEBI:60425	denotes	amyloid
T5954	31741-31747	GO:0030424	denotes	axonal
T5955	31741-31757	GO:0008088	denotes	axonal transport
T5956	31761-31764	PR:000004168	denotes	APP
T5957	31788-31796	UBERON:0001851	denotes	cortical
T5958	31788-31804	CL:0010012	denotes	cortical neurons
T5959	31812-31820	UBERON:0001897	denotes	thalamus
T5960	31868-31872	NCBITaxon:10088	denotes	mice
T5961	31874-31884	GO:0010467	denotes	expressing
T5962	31902-31905	PR:000004168	denotes	APP
T5963	31906-31916	SO:0000902	denotes	transgenes
T5964	31928-31935	CHEBI:60425	denotes	amyloid
T5965	31963-31967	NCBITaxon:10088	denotes	mice
T5966	32039-32046	CHEBI:60425	denotes	amyloid
T5967	32106-32113	NCBITaxon:33208	denotes	animals
T5968	32114-32118	GO:0007567	denotes	born
T5969	32133-32136	CHEBI:50845	denotes	dox
T5970	32138-32145	NCBITaxon:33208	denotes	Animals
T5971	32163-32173	GO:0010467	denotes	expressing
T5972	32204-32207	CHEBI:50845	denotes	dox
T5973	32234-32241	CHEBI:60425	denotes	amyloid
T5974	32306-32315	SO:0000902	denotes	transgene
T5975	32316-32326	GO:0010467	denotes	expression
T5976	32346-32349	CHEBI:50845	denotes	dox
T5977	32399-32406	CHEBI:60425	denotes	amyloid
T5978	32480-32490	CHEBI:35222	denotes	inhibitors
T5979	32494-32507	GO:0034205	denotes	Aβ production
T5980	32551-32555	NCBITaxon:10088	denotes	mice
T5981	32557-32564	PR:000003199	denotes	CaMKIIα
T5982	32571-32574	PR:000004168	denotes	APP
T5983	32595-32599	CHEBI:33290	denotes	food
T5984	32632-32639	CHEBI:60425	denotes	amyloid
T5985	32683-32688	UBERON:0000955	denotes	brain
T5986	32711-32718	NCBITaxon:33208	denotes	animals
T5987	32745-32749	CHEBI:33290	denotes	chow
T5988	32753-32757	CHEBI:33290	denotes	food
T5989	32769-32772	CHEBI:50845	denotes	dox
T5990	32857-32864	NCBITaxon:33208	denotes	animals
T5991	32887-32891	CHEBI:33290	denotes	chow
T5992	33113-33116	CHEBI:50845	denotes	dox
T5993	33125-33132	NCBITaxon:33208	denotes	animals
T5994	33192-33201	SO:0000902	denotes	transgene
T5995	33245-33249	NCBITaxon:10088	denotes	mice
T5996	33306-33313	NCBITaxon:33208	denotes	animals
T5997	33356-33363	NCBITaxon:33208	denotes	animals
T5998	33369-33372	CHEBI:50845	denotes	dox
T5999	33433-33436	PR:000004168	denotes	APP
T6000	35387-35393	UBERON:0000479	denotes	Tissue
T6001	35413-35419	NCBITaxon:33208	denotes	animal
T6002	35466-35473	CHEBI:60425	denotes	amyloid
T6003	35628-35635	CHEBI:60425	denotes	amyloid
T6004	35700-35707	CHEBI:60425	denotes	amyloid
T6005	35743-35750	CHEBI:60425	denotes	amyloid
T6006	35764-35768	NCBITaxon:10088	denotes	mice
T6007	36033-36040	NCBITaxon:33208	denotes	animals
T6008	36055-36064	SO:0000902	denotes	transgene
T6009	36185-36192	CHEBI:60425	denotes	amyloid
T6010	36329-36336	CHEBI:60425	denotes	amyloid
T6011	36444-36447	CHEBI:50845	denotes	dox
T6012	36482-36485	PR:000004168	denotes	APP
T6013	38308-38312	NCBITaxon:10088	denotes	mice
T6014	38330-38333	CHEBI:50845	denotes	dox
T6015	38385-38388	PR:000004168	denotes	APP
T6016	38389-38393	NCBITaxon:10088	denotes	mice
T6017	38395-38402	PR:000003199	denotes	CaMKIIα
T6018	38459-38462	CHEBI:50845	denotes	dox
T6019	38503-38507	NCBITaxon:10088	denotes	mice
T6020	38537-38546	SO:0000902	denotes	transgene
T6021	38654-38657	PR:000004168	denotes	APP
T6022	38673-38682	SO:0000902	denotes	transgene
T6023	38710-38717	NCBITaxon:33208	denotes	animals
T6024	38750-38754	NCBITaxon:10088	denotes	mice
T6025	38772-38779	CHEBI:60425	denotes	amyloid
T6026	38827-38831	NCBITaxon:10088	denotes	mice
T6027	38874-38883	SO:0000902	denotes	transgene
T6028	38884-38894	GO:0010467	denotes	expression
T6029	39075-39078	CHEBI:50845	denotes	dox
T6030	39087-39094	NCBITaxon:33208	denotes	animals
T6031	39157-39163	UBERON:0000955	denotes	brains
T6032	39171-39175	NCBITaxon:10088	denotes	mice
T6033	39193-39202	SO:0000902	denotes	transgene
T6034	39245-39253	UBERON:0001851	denotes	cortical
T6035	39254-39260	UBERON:0000479	denotes	tissue
T6036	39271-39277	NCBITaxon:33208	denotes	animal
T6037	39522-39528	GO:0007601	denotes	visual
T6038	39581-39584	PR:000004168	denotes	APP
T6039	39585-39589	NCBITaxon:10088	denotes	mice
T6040	39603-39606	CHEBI:50845	denotes	dox
T6041	39756-39760	NCBITaxon:10088	denotes	mice
T6042	39869-39872	PR:000004168	denotes	APP
T6043	39873-39877	NCBITaxon:10088	denotes	mice
T6044	39955-39962	NCBITaxon:33208	denotes	animals
T6045	40016-40025	SO:0000902	denotes	transgene
T6046	40106-40112	UBERON:0000955	denotes	brains
T6047	40224-40234	GO:0010467	denotes	expression
T6048	40251-40259	UBERON:0001851	denotes	Cortical
T6049	40331-40334	CHEBI:8984	denotes	SDS
T6050	40341-40343	CHEBI:30751	denotes	FA
T6051	40368-40377	SO:0000902	denotes	transgene
T6052	40417-40422	NCBITaxon:9606	denotes	human
T6053	40451-40458	NCBITaxon:33208	denotes	animals
T6054	40469-40476	CHEBI:60425	denotes	amyloid
T6055	40555-40558	CHEBI:8984	denotes	SDS
T6056	40563-40565	CHEBI:30751	denotes	FA
T6057	40694-40697	CHEBI:8984	denotes	SDS
T6058	40702-40704	CHEBI:30751	denotes	FA
T6059	40826-40832	UBERON:0000955	denotes	brains
T6060	40956-40960	NCBITaxon:10088	denotes	mice
T6061	41301-41308	NCBITaxon:33208	denotes	animals
T6062	41365-41372	NCBITaxon:33208	denotes	animals
T6063	43545-43553	GO:0043005	denotes	neuritic
T6064	43558-43563	CL:0000125	denotes	glial
T6103	46566-46570	NCBITaxon:10088	denotes	mice
T6105	46847-46850	PR:000004168	denotes	APP
T6106	46851-46855	NCBITaxon:10088	denotes	mice
T6107	46980-46989	SO:0000902	denotes	transgene
T6108	47114-47117	PR:000004168	denotes	APP
T6109	47118-47122	NCBITaxon:10088	denotes	mice
T6110	47126-47129	CHEBI:50845	denotes	dox
T6111	47131-47138	NCBITaxon:33208	denotes	Animals
T6112	47139-47143	GO:0007567	denotes	born
T6113	47158-47161	CHEBI:50845	denotes	dox
T6114	47257-47260	CHEBI:50845	denotes	dox
T6115	47268-47275	NCBITaxon:33208	denotes	animals
T6116	47323-47340	GO:0007623	denotes	circadian rhythms
T9940	48181-48186	NCBITaxon:10088	denotes	mouse
T9941	48257-48270	GO:0034205	denotes	Aβ production
T9942	48290-48297	CHEBI:60425	denotes	amyloid
T9943	48333-48337	NCBITaxon:10088	denotes	mice
T9944	48373-48380	GO:0010467	denotes	express
T9945	48417-48424	GO:0065007	denotes	control
T9946	48430-48442	CHEBI:27902	denotes	tetracycline
T9947	48454-48462	SO:0000167	denotes	promoter
T9948	48499-48502	CHEBI:50845	denotes	dox
T9949	48673-48683	GO:0010467	denotes	expression
T9950	48754-48761	CHEBI:60425	denotes	amyloid
T9951	48786-48790	NCBITaxon:10088	denotes	mice
T9952	48842-48849	CHEBI:60425	denotes	Amyloid
T9953	48922-48928	UBERON:0001851	denotes	cortex
T9954	48942-48946	NCBITaxon:10088	denotes	mice
T9955	49030-49033	PR:000004168	denotes	APP
T9956	49085-49092	CHEBI:60425	denotes	amyloid
T9957	49140-49147	NCBITaxon:33208	denotes	animals
T9958	49179-49186	CHEBI:60425	denotes	amyloid
T9959	49285-49295	CHEBI:35222	denotes	inhibitors
T9960	49369-49376	CHEBI:60425	denotes	amyloid
T9961	49403-49412	SO:0000902	denotes	transgene
T9962	49576-49586	PR:000008840	denotes	huntingtin
T9963	49646-49659	GO:0005576	denotes	extracellular
T9964	49660-49667	CHEBI:60425	denotes	amyloid
T9965	49697-49711	CHEBI:52217	denotes	pharmaceutical
T9966	49712-49723	GO:0070765	denotes	γ-secretase
T9967	49724-49734	CHEBI:35222	denotes	inhibitors
T9968	49770-49797	GO:1902003	denotes	regulation of Aβ production
T9969	49875-49884	CHEBI:36357	denotes	compounds
T9970	49935-49939	NCBITaxon:10088	denotes	mice
T9971	50117-50120	PR:000004168	denotes	APP
T9972	50121-50131	GO:0010467	denotes	expression
T9973	50146-50150	NCBITaxon:10088	denotes	mice
T9974	50237-50250	GO:0034205	denotes	Aβ production
T9975	50317-50328	GO:0070765	denotes	γ-secretase
T9976	50329-50339	CHEBI:35222	denotes	inhibitors
T9977	50377-50398	CHEBI:52217	denotes	pharmaceutical agents
T9978	50436-50460	GO:1902003	denotes	control of Aβ production
T9979	50562-50571	CHEBI:36357	denotes	compounds
T9980	50601-50608	CHEBI:60425	denotes	amyloid
T9981	50687-50694	CHEBI:60425	denotes	amyloid
T9982	50738-50747	SO:0000902	denotes	transgene
T9983	50791-50798	NCBITaxon:33208	denotes	animals
T9984	50814-50817	CHEBI:50845	denotes	dox
T9985	50871-50878	CHEBI:60425	denotes	amyloid
T9986	51016-51023	NCBITaxon:33208	denotes	animals
T9987	51037-51040	CHEBI:50845	denotes	dox
T9988	51286-51299	GO:0034205	denotes	Aβ production
T9989	51363-51370	CHEBI:60425	denotes	amyloid
T9990	51414-51423	UBERON:0000104	denotes	life span
T9991	51493-51499	NCBITaxon:9606	denotes	humans
T9992	51568-51577	UBERON:0000104	denotes	life span
T9993	51581-51587	NCBITaxon:9989	denotes	rodent
T9994	51789-51805	GO:0034205	denotes	production of Aβ
T9995	51861-51864	PR:000004168	denotes	APP
T9996	51865-51875	GO:0010467	denotes	expression
T9997	51974-51981	CHEBI:60425	denotes	amyloid
T9998	52079-52083	NCBITaxon:10088	denotes	mice
T9999	52096-52099	PR:000004168	denotes	APP
T10000	52286-52291	NCBITaxon:9606	denotes	human
T10001	52374-52378	NCBITaxon:10088	denotes	mice
T10002	52529-52538	SO:0000902	denotes	transgene
T10003	52539-52549	GO:0010467	denotes	expression
T10004	52579-52582	CHEBI:50845	denotes	dox
T10005	52603-52608	NCBITaxon:10088	denotes	mouse
T10006	52696-52701	NCBITaxon:10088	denotes	mouse
T10007	52723-52728	NCBITaxon:9606	denotes	human
T10008	52853-52860	CHEBI:60425	denotes	amyloid
T10009	52875-52882	PR:000003199	denotes	CaMKIIα
T10010	52903-52907	NCBITaxon:10088	denotes	mice
T10011	52918-52921	CHEBI:50845	denotes	dox
T10012	52954-52961	CHEBI:60425	denotes	amyloid
T10013	53018-53025	SO:0000704	denotes	genetic
T10014	53053-53069	GO:0034205	denotes	production of Aβ
T10015	53093-53099	NCBITaxon:9606	denotes	humans
T10016	53247-53250	PR:000004168	denotes	APP
T10017	53247-53250	_FRAGMENT	denotes	APP
T10018	53254-53263	GO:0042983	denotes	synthetic
T10019	53251-53263	GO:0034205	denotes	Aβ synthetic
T10020	53409-53416	CHEBI:60425	denotes	amyloid
T10021	53466-53473	CHEBI:60425	denotes	amyloid
T10022	53486-53491	NCBITaxon:9606	denotes	human
T10023	53492-53497	UBERON:0000955	denotes	brain
T10024	53555-53560	NCBITaxon:10088	denotes	mouse
T10025	53561-53566	UBERON:0000955	denotes	brain
T10026	53581-53586	NCBITaxon:9606	denotes	human
T10027	53587-53592	UBERON:0000955	denotes	brain
T10028	53639-53643	NCBITaxon:10088	denotes	mice
T10029	53699-53706	CHEBI:60425	denotes	amyloid
T10030	53741-53748	CHEBI:60425	denotes	amyloid
T10031	53788-53793	NCBITaxon:9606	denotes	human
T10032	53814-53819	NCBITaxon:10088	denotes	mouse
T10033	53838-53848	CL:0000129	denotes	microglial
T10034	53849-53861	GO:0006909	denotes	phagocytosis
T10035	53872-53881	CL:0000129	denotes	microglia
T10036	53896-53901	NCBITaxon:10088	denotes	mouse
T10037	53921-53927	UBERON:0000479	denotes	tissue
T10038	53976-53983	CHEBI:60425	denotes	amyloid
T10039	54017-54026	CL:0000129	denotes	microglia
T10040	54039-54046	CHEBI:60425	denotes	amyloid
T10041	54058-54063	NCBITaxon:9606	denotes	human
T10042	54064-54069	UBERON:0000955	denotes	brain
T10043	54122-54132	GO:0010467	denotes	expression
T10044	54180-54189	CL:0000129	denotes	microglia
T10045	54193-54200	CHEBI:60425	denotes	amyloid
T10046	54201-54211	GO:0008152	denotes	metabolism
T10047	54258-54263	NCBITaxon:9606	denotes	human
T10048	54355-54378	CHEBI:35472	denotes	anti-inflammatory drugs
T10049	54389-54399	CL:0000129	denotes	microglial
T10050	54389-54410	GO:0001774	denotes	microglial activation
T10051	54427-54434	CHEBI:60425	denotes	amyloid
T10052	54443-54446	PR:000004168	denotes	APP
T10053	54458-54462	NCBITaxon:10088	denotes	mice
T10054	54486-54491	NCBITaxon:10088	denotes	mouse
T10055	54492-54501	CL:0000129	denotes	microglia
T10056	54538-54545	CHEBI:60425	denotes	amyloid
T10057	54645-54668	CHEBI:35472	denotes	anti-inflammatory drugs
T10058	54672-54683	GO:0070765	denotes	γ-secretase
T10059	54684-54692	MOP:0000780	denotes	cleavage
T10060	54727-54736	CL:0000129	denotes	microglia
T10061	54749-54754	NCBITaxon:9606	denotes	human
T10062	54773-54778	NCBITaxon:10088	denotes	mouse
T10063	54827-54837	CL:0000129	denotes	microglial
T10064	54900-54907	CHEBI:60425	denotes	amyloid
T10065	54925-54931	UBERON:0000955	denotes	brains
T10066	54983-54986	PR:000004168	denotes	APP
T10067	54987-54991	NCBITaxon:10088	denotes	mice
T10068	55035-55044	CL:0000129	denotes	microglia
T10069	55054-55059	NCBITaxon:10088	denotes	mouse
T10070	55149-55156	CHEBI:60425	denotes	amyloid
T10071	55182-55185	PR:000004168	denotes	APP
T10072	55186-55190	NCBITaxon:10088	denotes	mice
T10073	55244-55247	CHEBI:50845	denotes	dox
T10074	55299-55308	CL:0000129	denotes	microglia
T10075	55324-55328	NCBITaxon:10088	denotes	mice
T10076	55330-55333	CHEBI:50845	denotes	Dox
T10077	55361-55372	CHEBI:50694	denotes	minocycline
T10078	55385-55407	CHEBI:35472	denotes	anti-inflammatory drug
T10079	55412-55421	CHEBI:35222	denotes	inhibitor
T10080	55425-55435	CL:0000129	denotes	microglial
T10081	55425-55446	GO:0001774	denotes	microglial activation
T10082	55465-55468	CHEBI:50845	denotes	dox
T10083	55550-55569	CHEBI:67079	denotes	anti-inflammatories
T10084	55607-55614	CHEBI:60425	denotes	amyloid
T10085	55622-55625	CHEBI:50845	denotes	dox
T10086	55634-55641	NCBITaxon:33208	denotes	animals
T10087	55701-55704	CHEBI:50845	denotes	dox
T10088	55736-55743	CHEBI:60425	denotes	amyloid
T10089	55799-55809	CL:0000129	denotes	microglial
T10090	55845-55850	NCBITaxon:10088	denotes	mouse
T10091	55899-55902	CHEBI:50845	denotes	dox
T10092	55912-55922	CL:0000129	denotes	microglial
T10093	56002-56009	CHEBI:60425	denotes	amyloid
T10094	56109-56114	NCBITaxon:10088	denotes	mouse
T10095	56159-56169	GO:0042571	denotes	antibodies
T10096	56197-56202	UBERON:0000955	denotes	brain
T10097	56232-56239	CHEBI:60425	denotes	amyloid
T10098	56306-56314	GO:0042571	denotes	antibody
T10099	56335-56342	CHEBI:60425	denotes	amyloid
T10100	56457-56464	CHEBI:60425	denotes	amyloid
T10101	56566-56576	NCBITaxon:11646	denotes	lentiviral
T10102	56589-56599	PR:000001898	denotes	neprilysin
T10103	56734-56742	GO:0042571	denotes	antibody
T10104	56781-56800	CL:0002629	denotes	activated microglia
T10105	56977-56987	CL:0000129	denotes	microglial
T10106	57004-57012	GO:0042571	denotes	antibody
T10107	57013-57020	CHEBI:59132	denotes	antigen
T10108	57021-57030	GO:0032991	denotes	complexes
T10109	57035-57038	PR:000004168	denotes	APP
T10110	57050-57055	NCBITaxon:10088	denotes	mouse
T10111	57101-57109	GO:0042571	denotes	antibody
T10112	57179-57188	CL:0000129	denotes	microglia
T10113	57179-57199	GO:0001774	denotes	microglia activation
T10114	57296-57309	UBERON:0002405	denotes	immune system
T10115	57378-57386	GO:0042571	denotes	antibody
T10116	57390-57395	NCBITaxon:10239	denotes	viral
T10117	57426-57449	GO:0001774	denotes	activation of microglia
T10118	57440-57449	CL:0000129	denotes	microglia
T10119	57528-57540	GO:0006909	denotes	phagocytosis
T10120	57595-57605	PR:000001898	denotes	neprilysin
T10121	57621-57631	GO:0042571	denotes	antibodies
T10122	57728-57751	GO:0001774	denotes	activation of microglia
T10123	57742-57751	CL:0000129	denotes	microglia
T10124	57771-57781	GO:0010467	denotes	expression
T10125	57785-57789	PR:000000046	denotes	TGFβ
T10126	57819-57837	CHEBI:16412	denotes	lipopolysaccharide
T10127	57898-57901	PR:000004168	denotes	APP
T10128	57913-57917	NCBITaxon:10088	denotes	mice
T10129	57944-57952	GO:0042571	denotes	antibody
T10130	58031-58039	GO:0042571	denotes	antibody
T10131	58132-58139	CHEBI:60425	denotes	amyloid
T10132	58244-58253	CL:0000129	denotes	microglia
T10133	58257-58262	NCBITaxon:10088	denotes	mouse
T10134	58343-58349	NCBITaxon:9606	denotes	humans
T10135	58398-58402	NCBITaxon:10088	denotes	mice
T10136	58408-58415	GO:0010467	denotes	express
T10137	58422-58427	NCBITaxon:9606	denotes	human
T10138	58428-58431	PR:000010173	denotes	tau
T10139	58446-58452	SO:0000440	denotes	vector
T10140	58484-58487	PR:000004168	denotes	APP
T10141	58488-58492	NCBITaxon:10088	denotes	mice
T10142	58522-58525	PR:000010173	denotes	tau
T10143	58522-58549	GO:0097418	denotes	tau neurofibrillary tangles
T10144	58556-58563	CHEBI:60425	denotes	amyloid
T10145	58611-58620	SO:0000902	denotes	transgene
T10146	58781-58786	NCBITaxon:10088	denotes	mouse
T10147	58907-58910	CHEBI:50845	denotes	dox
T10148	58920-58929	SO:0000902	denotes	transgene
T10149	59186-59189	PR:000010173	denotes	tau
T10150	59275-59281	_FRAGMENT	denotes	intra-
T10151	59291-59299	GO:0005622	denotes	cellular
T10152	59286-59299	GO:0005576	denotes	extracellular
T10153	59399-59411	_FRAGMENT	denotes	synthesis of
T10154	59416-59418	GO:0034205	denotes	Aβ
T10155	59444-59453	GO:0050890	denotes	cognitive
T10156	59488-59491	PR:000010173	denotes	tau
T10157	59492-59496	NCBITaxon:10088	denotes	mice
T10158	59560-59569	GO:0050890	denotes	cognitive
T10159	59592-59601	SO:0000902	denotes	transgene
T10160	59657-59666	GO:0050890	denotes	cognitive
T10161	59729-59732	PR:000004168	denotes	APP
T10162	59733-59737	NCBITaxon:10088	denotes	mice
T10163	59797-59804	CHEBI:60425	denotes	amyloid
T10164	59834-59837	PR:000004168	denotes	APP
T10165	59838-59842	NCBITaxon:10088	denotes	mice
T10166	59998-60002	NCBITaxon:10088	denotes	mice
T10167	60039-60047	GO:0036268	denotes	swimming
T10168	60100-60105	CHEBI:15377	denotes	water
T10169	60165-60173	GO:0007612	denotes	learning
T10170	60178-60184	GO:0007613	denotes	memory
T10171	60303-60312	GO:0050890	denotes	cognitive
T10172	60411-60421	GO:0010467	denotes	expression
T10173	60459-60470	GO:0006508	denotes	proteolytic
T10174	60560-60563	CHEBI:50845	denotes	dox
T10175	60571-60575	NCBITaxon:10088	denotes	mice
T10176	60737-60740	PR:000004168	denotes	APP
T10177	60737-60750	GO:0042983	denotes	APP synthesis
T10178	60852-60860	CL:0000540	denotes	neuronal
T10179	60880-60889	SO:0000902	denotes	transgene
T10180	60890-60900	GO:0010467	denotes	expression
T10181	60918-60923	GO:0007567	denotes	natal
T10182	61055-61068	GO:0034205	denotes	Aβ production
T10183	61129-61134	NCBITaxon:10088	denotes	mouse
T10184	61212-61224	GO:0034205	denotes	Aβ synthesis
T10185	61226-61234	GO:0043005	denotes	neuritic
T10186	61363-61376	GO:0034205	denotes	Aβ production
T10187	61415-61427	GO:0006909	denotes	phagocytosis
T10188	61441-61448	CHEBI:60425	denotes	amyloid
T10189	61572-61582	CHEBI:35222	denotes	inhibitors
T10190	61665-61672	CHEBI:60425	denotes	amyloid
T10191	61750-61760	CL:0000129	denotes	microglial
T10192	61750-61771	GO:0001774	denotes	microglial activation
T10193	61775-61780	NCBITaxon:9606	denotes	human
T10194	61839-61846	CHEBI:60425	denotes	amyloid
T10195	61874-61886	GO:0006909	denotes	phagocytosis
T10196	61977-61987	CHEBI:35222	denotes	inhibitors
T10197	62041-62046	NCBITaxon:9606	denotes	human
T10198	62047-62052	UBERON:0000955	denotes	brain
T10199	62080-62087	CHEBI:60425	denotes	amyloid
T6081	45105-45112	NCBITaxon:33208	denotes	animals
T6082	45199-45207	GO:0036268	denotes	swimming
T6083	45235-45239	NCBITaxon:10088	denotes	mice
T6084	45266-45271	CHEBI:15377	denotes	water
T6085	45292-45297	CHEBI:15377	denotes	water
T6086	45315-45319	GO:0036268	denotes	swim
T6087	45446-45453	NCBITaxon:33208	denotes	animals
T6088	45524-45528	NCBITaxon:10088	denotes	mice
T6089	45666-45673	NCBITaxon:33208	denotes	animals
T6090	45732-45736	NCBITaxon:10088	denotes	mice
T6091	45788-45795	GO:0010467	denotes	express
T6092	45938-45945	CHEBI:60425	denotes	amyloid
T6093	45955-45964	GO:0050890	denotes	cognitive
T6094	46064-46067	PR:000004168	denotes	APP
T6095	46068-46072	NCBITaxon:10088	denotes	mice
T6096	46122-46131	GO:0050890	denotes	cognitive
T6097	46148-46155	NCBITaxon:33208	denotes	animals
T6098	46205-46214	GO:0050890	denotes	cognitive
T6099	46306-46309	PR:000004168	denotes	APP
T6100	46310-46314	NCBITaxon:10088	denotes	mice
T6101	46373-46380	NCBITaxon:33208	denotes	animals
T6102	46486-46496	GO:0040011	denotes	ambulation
T6104	46734-46747	GO:0007623	denotes	diurnal cycle
T5825	21102-21111	SO:0000902	denotes	transgene
T6065	43679-43688	SO:0000902	denotes	transgene
T6066	43763-43771	GO:0043005	denotes	neuritic
T6067	43829-43849	CL:0002627	denotes	activated astrocytes
T6068	43868-43872	PR:000007939	denotes	GFAP
T6069	43904-43911	NCBITaxon:33208	denotes	animals
T6070	43924-43932	GO:0043005	denotes	Neuritic
T6071	43937-43942	CL:0000125	denotes	glial
T6072	43993-43997	NCBITaxon:10088	denotes	mice
T6073	44012-44021	SO:0000902	denotes	transgene
T6074	44100-44104	NCBITaxon:10088	denotes	mice
T6075	44851-44860	GO:0050890	denotes	cognitive
T6076	44882-44886	NCBITaxon:10088	denotes	mice
T6077	44913-44922	GO:0050890	denotes	cognition
T6078	44996-45005	GO:0050890	denotes	cognitive
T6079	45087-45091	NCBITaxon:10088	denotes	mice
T6080	45101-45104	PR:000004168	denotes	APP
R6980	T10018	T10017	_lexicallyChainedTo	synthetic,APP
R6981	T10151	T10150	_lexicallyChainedTo	cellular,intra-
R6982	T10154	T10153	_lexicallyChainedTo	Aβ,synthesis of
R1620	T2388	T2387	_lexicallyChainedTo	antibiotic,breakdown of
R1714	T2531	T2530	_lexicallyChainedTo	DNA,amplification of
R1715	T2558	T2557	_lexicallyChainedTo	gene,segment of
R320	T555	T554	_lexicallyChainedTo	2,presenilins

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