Id |
Subject |
Object |
Predicate |
Lexical cue |
T14252 |
0-10 |
NN |
denotes |
Immunoblot |
T14253 |
11-19 |
NN |
denotes |
analysis |
T14254 |
19-128 |
sentence |
denotes |
Mouse brains were removed, and the hippocampus, olfactory bulb, and remaining forebrain areas were isolated. |
T14255 |
20-25 |
NN |
denotes |
Mouse |
T14256 |
26-32 |
NNS |
denotes |
brains |
T14257 |
38-45 |
VBN |
denotes |
removed |
T14258 |
33-37 |
VBD |
denotes |
were |
T14259 |
45-47 |
, |
denotes |
, |
T14260 |
47-50 |
CC |
denotes |
and |
T14261 |
51-54 |
DT |
denotes |
the |
T14262 |
55-66 |
NN |
denotes |
hippocampus |
T14263 |
119-127 |
VBN |
denotes |
isolated |
T14264 |
66-68 |
, |
denotes |
, |
T14265 |
68-77 |
JJ |
denotes |
olfactory |
T14266 |
78-82 |
NN |
denotes |
bulb |
T14267 |
82-84 |
, |
denotes |
, |
T14268 |
84-87 |
CC |
denotes |
and |
T14269 |
88-97 |
VBG |
denotes |
remaining |
T14270 |
108-113 |
NNS |
denotes |
areas |
T14271 |
98-107 |
NN |
denotes |
forebrain |
T14272 |
114-118 |
VBD |
denotes |
were |
T14273 |
127-128 |
. |
denotes |
. |
T14274 |
128-206 |
sentence |
denotes |
Total protein was prepared, and immunoblots were performed as described [87]. |
T14275 |
129-134 |
JJ |
denotes |
Total |
T14276 |
135-142 |
NN |
denotes |
protein |
T14277 |
147-155 |
VBN |
denotes |
prepared |
T14278 |
143-146 |
VBD |
denotes |
was |
T14279 |
155-157 |
, |
denotes |
, |
T14280 |
157-160 |
CC |
denotes |
and |
T14281 |
161-172 |
NNS |
denotes |
immunoblots |
T14282 |
178-187 |
VBN |
denotes |
performed |
T14283 |
173-177 |
VBD |
denotes |
were |
T14284 |
188-190 |
IN |
denotes |
as |
T14285 |
191-200 |
VBN |
denotes |
described |
T14286 |
201-202 |
-LRB- |
denotes |
[ |
T14287 |
202-204 |
CD |
denotes |
87 |
T14288 |
204-205 |
-RRB- |
denotes |
] |
T14289 |
205-206 |
. |
denotes |
. |
T14290 |
206-454 |
sentence |
denotes |
Antibodies used were against GluR-B (1:800, monoclonal; Chemicon), β-actin (1:40,000, monoclonal; Sigma, St. Louis, Missouri, United States) as an internal standard, and secondary goat anti-rabbit and goat anti-mouse antibodies (Vector, 1:15,000). |
T14291 |
207-217 |
NNS |
denotes |
Antibodies |
T14292 |
223-227 |
VBD |
denotes |
were |
T14293 |
218-222 |
VBN |
denotes |
used |
T14294 |
228-235 |
IN |
denotes |
against |
T14295 |
236-240 |
NN |
denotes |
GluR |
T14296 |
241-242 |
NN |
denotes |
B |
T14297 |
240-241 |
HYPH |
denotes |
- |
T14298 |
243-244 |
-LRB- |
denotes |
( |
T14299 |
263-271 |
NNP |
denotes |
Chemicon |
T14300 |
244-245 |
CD |
denotes |
1 |
T14301 |
251-261 |
JJ |
denotes |
monoclonal |
T14302 |
245-246 |
SYM |
denotes |
: |
T14303 |
246-249 |
CD |
denotes |
800 |
T14304 |
249-251 |
, |
denotes |
, |
T14305 |
261-262 |
: |
denotes |
; |
T14306 |
271-272 |
-RRB- |
denotes |
) |
T14307 |
272-274 |
, |
denotes |
, |
T14308 |
274-275 |
NN |
denotes |
β |
T14309 |
276-281 |
NN |
denotes |
actin |
T14310 |
275-276 |
HYPH |
denotes |
- |
T14311 |
282-283 |
-LRB- |
denotes |
( |
T14312 |
305-310 |
NNP |
denotes |
Sigma |
T14313 |
283-284 |
CD |
denotes |
1 |
T14314 |
293-303 |
JJ |
denotes |
monoclonal |
T14315 |
284-285 |
SYM |
denotes |
: |
T14316 |
285-291 |
CD |
denotes |
40,000 |
T14317 |
291-293 |
, |
denotes |
, |
T14318 |
303-304 |
: |
denotes |
; |
T14319 |
310-312 |
, |
denotes |
, |
T14320 |
312-315 |
NNP |
denotes |
St. |
T14321 |
316-321 |
NNP |
denotes |
Louis |
T14322 |
321-323 |
, |
denotes |
, |
T14323 |
323-331 |
NNP |
denotes |
Missouri |
T14324 |
331-333 |
, |
denotes |
, |
T14325 |
333-339 |
NNP |
denotes |
United |
T14326 |
340-346 |
NNP |
denotes |
States |
T14327 |
346-347 |
-RRB- |
denotes |
) |
T14328 |
348-350 |
IN |
denotes |
as |
T14329 |
351-353 |
DT |
denotes |
an |
T14330 |
363-371 |
NN |
denotes |
standard |
T14331 |
354-362 |
JJ |
denotes |
internal |
T14332 |
371-373 |
, |
denotes |
, |
T14333 |
373-376 |
CC |
denotes |
and |
T14334 |
377-386 |
JJ |
denotes |
secondary |
T14335 |
424-434 |
NNS |
denotes |
antibodies |
T14336 |
387-391 |
NN |
denotes |
goat |
T14337 |
392-403 |
JJ |
denotes |
anti-rabbit |
T14338 |
404-407 |
CC |
denotes |
and |
T14339 |
408-412 |
NN |
denotes |
goat |
T14340 |
413-423 |
JJ |
denotes |
anti-mouse |
T14341 |
435-436 |
-LRB- |
denotes |
( |
T14342 |
444-445 |
CD |
denotes |
1 |
T14343 |
436-442 |
NNP |
denotes |
Vector |
T14344 |
442-444 |
, |
denotes |
, |
T14345 |
445-446 |
SYM |
denotes |
: |
T14346 |
446-452 |
CD |
denotes |
15,000 |
T14347 |
452-453 |
-RRB- |
denotes |
) |
T14348 |
453-454 |
. |
denotes |
. |
T14349 |
454-612 |
sentence |
denotes |
Immunoreactivity was detected with ECLplus (Amersham, Little Chalfont, United Kingdom), and immunoblots were scanned and quantitatively analyzed with ImageJ. |
T14350 |
455-471 |
NN |
denotes |
Immunoreactivity |
T14351 |
476-484 |
VBN |
denotes |
detected |
T14352 |
472-475 |
VBD |
denotes |
was |
T14353 |
485-489 |
IN |
denotes |
with |
T14354 |
490-497 |
NNP |
denotes |
ECLplus |
T14355 |
498-499 |
-LRB- |
denotes |
( |
T14356 |
499-507 |
NNP |
denotes |
Amersham |
T14357 |
507-509 |
, |
denotes |
, |
T14358 |
509-515 |
NNP |
denotes |
Little |
T14359 |
516-524 |
NNP |
denotes |
Chalfont |
T14360 |
524-526 |
, |
denotes |
, |
T14361 |
526-532 |
NNP |
denotes |
United |
T14362 |
533-540 |
NNP |
denotes |
Kingdom |
T14363 |
540-541 |
-RRB- |
denotes |
) |
T14364 |
541-543 |
, |
denotes |
, |
T14365 |
543-546 |
CC |
denotes |
and |
T14366 |
547-558 |
NNS |
denotes |
immunoblots |
T14367 |
564-571 |
VBN |
denotes |
scanned |
T14368 |
559-563 |
VBD |
denotes |
were |
T14369 |
572-575 |
CC |
denotes |
and |
T14370 |
576-590 |
RB |
denotes |
quantitatively |
T14371 |
591-599 |
VBN |
denotes |
analyzed |
T14372 |
600-604 |
IN |
denotes |
with |
T14373 |
605-611 |
NNP |
denotes |
ImageJ |
T14374 |
611-612 |
. |
denotes |
. |
R9296 |
T14252 |
T14253 |
compound |
Immunoblot,analysis |
R9297 |
T14255 |
T14256 |
compound |
Mouse,brains |
R9298 |
T14256 |
T14257 |
nsubjpass |
brains,removed |
R9299 |
T14258 |
T14257 |
auxpass |
were,removed |
R9300 |
T14259 |
T14257 |
punct |
", ",removed |
R9301 |
T14260 |
T14257 |
cc |
and,removed |
R9302 |
T14261 |
T14262 |
det |
the,hippocampus |
R9303 |
T14262 |
T14263 |
nsubjpass |
hippocampus,isolated |
R9304 |
T14263 |
T14257 |
conj |
isolated,removed |
R9305 |
T14264 |
T14262 |
punct |
", ",hippocampus |
R9306 |
T14265 |
T14266 |
amod |
olfactory,bulb |
R9307 |
T14266 |
T14262 |
conj |
bulb,hippocampus |
R9308 |
T14267 |
T14266 |
punct |
", ",bulb |
R9309 |
T14268 |
T14266 |
cc |
and,bulb |
R9310 |
T14269 |
T14270 |
amod |
remaining,areas |
R9311 |
T14270 |
T14266 |
conj |
areas,bulb |
R9312 |
T14271 |
T14270 |
compound |
forebrain,areas |
R9313 |
T14272 |
T14263 |
auxpass |
were,isolated |
R9314 |
T14273 |
T14263 |
punct |
.,isolated |
R9315 |
T14275 |
T14276 |
amod |
Total,protein |
R9316 |
T14276 |
T14277 |
nsubjpass |
protein,prepared |
R9317 |
T14278 |
T14277 |
auxpass |
was,prepared |
R9318 |
T14279 |
T14277 |
punct |
", ",prepared |
R9319 |
T14280 |
T14277 |
cc |
and,prepared |
R9320 |
T14281 |
T14282 |
nsubjpass |
immunoblots,performed |
R9321 |
T14282 |
T14277 |
conj |
performed,prepared |
R9322 |
T14283 |
T14282 |
auxpass |
were,performed |
R9323 |
T14284 |
T14285 |
mark |
as,described |
R9324 |
T14285 |
T14282 |
advcl |
described,performed |
R9325 |
T14286 |
T14287 |
punct |
[,87 |
R9326 |
T14287 |
T14282 |
parataxis |
87,performed |
R9327 |
T14288 |
T14287 |
punct |
],87 |
R9328 |
T14289 |
T14282 |
punct |
.,performed |
R9329 |
T14291 |
T14292 |
nsubj |
Antibodies,were |
R9330 |
T14293 |
T14291 |
acl |
used,Antibodies |
R9331 |
T14294 |
T14292 |
prep |
against,were |
R9332 |
T14295 |
T14296 |
compound |
GluR,B |
R9333 |
T14296 |
T14294 |
pobj |
B,against |
R9334 |
T14297 |
T14296 |
punct |
-,B |
R9335 |
T14298 |
T14299 |
punct |
(,Chemicon |
R9336 |
T14299 |
T14296 |
parataxis |
Chemicon,B |
R9337 |
T14300 |
T14301 |
dep |
1,monoclonal |
R9338 |
T14301 |
T14299 |
dep |
monoclonal,Chemicon |
R9339 |
T14302 |
T14303 |
punct |
:,800 |
R9340 |
T14303 |
T14300 |
prep |
800,1 |
R9341 |
T14304 |
T14301 |
punct |
", ",monoclonal |
R9342 |
T14305 |
T14299 |
punct |
;,Chemicon |
R9343 |
T14306 |
T14299 |
punct |
),Chemicon |
R9344 |
T14307 |
T14296 |
punct |
", ",B |
R9345 |
T14308 |
T14309 |
compound |
β,actin |
R9346 |
T14309 |
T14296 |
appos |
actin,B |
R9347 |
T14310 |
T14309 |
punct |
-,actin |
R9348 |
T14311 |
T14312 |
punct |
(,Sigma |
R9349 |
T14312 |
T14309 |
parataxis |
Sigma,actin |
R9350 |
T14313 |
T14314 |
dep |
1,monoclonal |
R9351 |
T14314 |
T14312 |
dep |
monoclonal,Sigma |
R9352 |
T14315 |
T14316 |
punct |
:,"40,000" |
R9353 |
T14316 |
T14313 |
prep |
"40,000",1 |
R9354 |
T14317 |
T14314 |
punct |
", ",monoclonal |
R9355 |
T14318 |
T14312 |
punct |
;,Sigma |
R9356 |
T14319 |
T14312 |
punct |
", ",Sigma |
R9357 |
T14320 |
T14321 |
compound |
St.,Louis |
R9358 |
T14321 |
T14312 |
npadvmod |
Louis,Sigma |
R9359 |
T14322 |
T14312 |
punct |
", ",Sigma |
R9360 |
T14323 |
T14312 |
npadvmod |
Missouri,Sigma |
R9361 |
T14324 |
T14312 |
punct |
", ",Sigma |
R9362 |
T14325 |
T14326 |
compound |
United,States |
R9363 |
T14326 |
T14312 |
npadvmod |
States,Sigma |
R9364 |
T14327 |
T14312 |
punct |
),Sigma |
R9365 |
T14328 |
T14296 |
prep |
as,B |
R9366 |
T14329 |
T14330 |
det |
an,standard |
R9367 |
T14330 |
T14328 |
pobj |
standard,as |
R9368 |
T14331 |
T14330 |
amod |
internal,standard |
R9369 |
T14332 |
T14296 |
punct |
", ",B |
R9370 |
T14333 |
T14296 |
cc |
and,B |
R9371 |
T14334 |
T14335 |
amod |
secondary,antibodies |
R9372 |
T14335 |
T14296 |
conj |
antibodies,B |
R9373 |
T14336 |
T14335 |
nmod |
goat,antibodies |
R9374 |
T14337 |
T14336 |
amod |
anti-rabbit,goat |
R9375 |
T14338 |
T14336 |
cc |
and,goat |
R9376 |
T14339 |
T14336 |
conj |
goat,goat |
R9377 |
T14340 |
T14339 |
amod |
anti-mouse,goat |
R9378 |
T14341 |
T14342 |
punct |
(,1 |
R9379 |
T14342 |
T14335 |
parataxis |
1,antibodies |
R9380 |
T14343 |
T14342 |
dep |
Vector,1 |
R9381 |
T14344 |
T14342 |
punct |
", ",1 |
R9382 |
T14345 |
T14346 |
punct |
:,"15,000" |
R9383 |
T14346 |
T14342 |
prep |
"15,000",1 |
R9384 |
T14347 |
T14342 |
punct |
),1 |
R9385 |
T14348 |
T14292 |
punct |
.,were |
R9386 |
T14350 |
T14351 |
nsubjpass |
Immunoreactivity,detected |
R9387 |
T14352 |
T14351 |
auxpass |
was,detected |
R9388 |
T14353 |
T14351 |
prep |
with,detected |
R9389 |
T14354 |
T14353 |
pobj |
ECLplus,with |
R9390 |
T14355 |
T14356 |
punct |
(,Amersham |
R9391 |
T14356 |
T14354 |
parataxis |
Amersham,ECLplus |
R9392 |
T14357 |
T14356 |
punct |
", ",Amersham |
R9393 |
T14358 |
T14359 |
compound |
Little,Chalfont |
R9394 |
T14359 |
T14356 |
npadvmod |
Chalfont,Amersham |
R9395 |
T14360 |
T14356 |
punct |
", ",Amersham |
R9396 |
T14361 |
T14362 |
compound |
United,Kingdom |
R9397 |
T14362 |
T14356 |
npadvmod |
Kingdom,Amersham |
R9398 |
T14363 |
T14356 |
punct |
),Amersham |
R9399 |
T14364 |
T14351 |
punct |
", ",detected |
R9400 |
T14365 |
T14351 |
cc |
and,detected |
R9401 |
T14366 |
T14367 |
nsubj |
immunoblots,scanned |
R9402 |
T14367 |
T14351 |
conj |
scanned,detected |
R9403 |
T14368 |
T14367 |
aux |
were,scanned |
R9404 |
T14369 |
T14367 |
cc |
and,scanned |
R9405 |
T14370 |
T14371 |
advmod |
quantitatively,analyzed |
R9406 |
T14371 |
T14367 |
conj |
analyzed,scanned |
R9407 |
T14372 |
T14367 |
prep |
with,scanned |
R9408 |
T14373 |
T14372 |
pobj |
ImageJ,with |
R9409 |
T14374 |
T14367 |
punct |
.,scanned |