PMC:1253828 / 5734-5917
Annnotations
craft-sa-dev
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A neomycin resistance cassette (PGK-neo) flanked by two loxP sites was cloned into the SacI site 5′ of exon 3 of Mtf1, the third loxP site was cloned into the ScaI site 3′ of exon 4."}
craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T1979","span":{"begin":3,"end":11},"obj":"CHEBI:7507"},{"id":"T1980","span":{"begin":23,"end":31},"obj":"SO:0005853"},{"id":"T1981","span":{"begin":42,"end":49},"obj":"SO:0000357"},{"id":"T1982","span":{"begin":57,"end":67},"obj":"SO:0000346"},{"id":"T1983","span":{"begin":104,"end":108},"obj":"SO:0000147"},{"id":"T1984","span":{"begin":114,"end":118},"obj":"PR:000010717"},{"id":"T1985","span":{"begin":130,"end":139},"obj":"SO:0000346"},{"id":"T1986","span":{"begin":176,"end":180},"obj":"SO:0000147"}],"text":" A neomycin resistance cassette (PGK-neo) flanked by two loxP sites was cloned into the SacI site 5′ of exon 3 of Mtf1, the third loxP site was cloned into the ScaI site 3′ of exon 4."}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T2024","span":{"begin":23,"end":31},"obj":"SO_EXT:0005853"},{"id":"T2025","span":{"begin":33,"end":36},"obj":"GO_EXT:phosphoglycerate_kinase"},{"id":"T2026","span":{"begin":37,"end":40},"obj":"CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase"},{"id":"T2027","span":{"begin":42,"end":49},"obj":"SO:0000357"},{"id":"T2028","span":{"begin":57,"end":67},"obj":"SO_EXT:0000346"},{"id":"T2029","span":{"begin":72,"end":78},"obj":"SO_EXT:sequence_cloning_process"},{"id":"T2030","span":{"begin":104,"end":108},"obj":"SO_EXT:0000147"},{"id":"T2031","span":{"begin":114,"end":118},"obj":"PR_EXT:000010717"},{"id":"T2032","span":{"begin":130,"end":139},"obj":"SO_EXT:0000346"},{"id":"T2033","span":{"begin":144,"end":150},"obj":"SO_EXT:sequence_cloning_process"},{"id":"T2034","span":{"begin":176,"end":180},"obj":"SO_EXT:0000147"},{"id":"T2023","span":{"begin":3,"end":11},"obj":"CHEBI:7507"}],"text":" A neomycin resistance cassette (PGK-neo) flanked by two loxP sites was cloned into the SacI site 5′ of exon 3 of Mtf1, the third loxP site was cloned into the ScaI site 3′ of exon 4."}