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previous target gene search for MTF-1 with mouse embryos of conventional Mtf1 knockout phenotype revealed, besides metallothionein genes, the multifunctional alpha-fetoprotein (Afp) and the liver-enriched transcription factor CCAAT/enhancer binding protein alpha (Cebpa) as prime candidates (65). After an early onset during hepatogenesis, Afp expression is repressed postnatally and replaced by albumin (66). Thus, our adult Mtf1Mx-cre mice lacking MTF-1 were not suitable to analyze Afp expression. Cebpa is expressed in the adult liver as well as in other tissues (67), but the present microarray data revealed no significant expression differences in livers from adult Mtf1loxP and Mtf1Mx-cre mice (data not shown). Therefore, MTF-1 may affect Cebpa expression only during embryonal development, perhaps in combination with as yet unidentified factors."}

{"project":"2_test","denotations":[{"id":"16221973-11266553-77403470","span":{"begin":294,"end":296},"obj":"11266553"},{"id":"16221973-10202132-77403471","span":{"begin":407,"end":409},"obj":"10202132"},{"id":"16221973-12006103-77403472","span":{"begin":570,"end":572},"obj":"12006103"}],"text":"A previous target gene search for MTF-1 with mouse embryos of conventional Mtf1 knockout phenotype revealed, besides metallothionein genes, the multifunctional alpha-fetoprotein (Afp) and the liver-enriched transcription factor CCAAT/enhancer binding protein alpha (Cebpa) as prime candidates (65). After an early onset during hepatogenesis, Afp expression is repressed postnatally and replaced by albumin (66). Thus, our adult Mtf1Mx-cre mice lacking MTF-1 were not suitable to analyze Afp expression. Cebpa is expressed in the adult liver as well as in other tissues (67), but the present microarray data revealed no significant expression differences in livers from adult Mtf1loxP and Mtf1Mx-cre mice (data not shown). Therefore, MTF-1 may affect Cebpa expression only during embryonal development, perhaps in combination with as yet unidentified factors."}

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