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genes involved in the metabolism of the antioxidant glutathione were found to be upregulated by cadmium exposure, namely the genes encoding the catalytic subunit of glutamate-cysteine ligase (Gclc) that is the rate limiting enzyme in de novo synthesis of glutathione (45); glutathione reductase 1 (Gsr), the reducing enzyme for oxidized glutathione (45); and glutathione-S-transferase, mu 4 (Gstm4), which is a member of the glutathione-S-transferase supergene family of detoxification enzymes (45). 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2_test
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craft-ca-core-dev
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craft-ca-core-ex-dev
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In all of these cases, induction was confirmed by semiquantitative RT–PCRs (data not shown). Gclc, also referred to as heavy chain subunit of gamma-glutamylcysteine synthetase (Ggcs-hc), had been discussed previously as a target gene of MTF-1 (6). Our expression data indicate that Gclc is induced by cadmium but, at least in the adult mouse liver, not dependent on MTF-1."}