PMC:1253828 / 14430-15039
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2_test
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craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T4430","span":{"begin":72,"end":78},"obj":"UBERON:0000479"},{"id":"T4431","span":{"begin":212,"end":242},"obj":"CHEBI:28714"},{"id":"T4432","span":{"begin":244,"end":247},"obj":"CHEBI:28714"},{"id":"T4433","span":{"begin":260,"end":264},"obj":"CHEBI:23888"},{"id":"T4434","span":{"begin":279,"end":290},"obj":"CHEBI:16856"},{"id":"T4435","span":{"begin":279,"end":300},"obj":"GO:0006750"},{"id":"T4436","span":{"begin":354,"end":359},"obj":"CHEBI:35456"},{"id":"T4437","span":{"begin":458,"end":461},"obj":"CHEBI:53233"},{"id":"T4438","span":{"begin":463,"end":523},"obj":"CHEBI:53233"},{"id":"T4439","span":{"begin":531,"end":549},"obj":"GO:0008283"}],"text":"Cytotoxicity assay\nSamples of 1 × 104 cells/well were plated in 96-well tissue culture plates and allowed to adhere for 24 h. The cells were then pre-incubated for 24 h in medium containing 0, 5, 10, 25 or 50 µM l-buthionine-[S,R]-sulfoximine (BSO) (Sigma), a drug that inhibits glutathione synthesis (31). Later, cells were exposed to 0, 5, 10 or 20 µM CdCl2 in the specified pre-incubation medium for an additional 24 h. Cytotoxicity was determined by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromid)-based Cell Proliferation Kit I (Roche) according to the manufacturer's instructions."}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T4440","span":{"begin":38,"end":43},"obj":"CL_GO_EXT:cell"},{"id":"T4441","span":{"begin":72,"end":78},"obj":"UBERON:0000479"},{"id":"T4442","span":{"begin":109,"end":115},"obj":"GO_PATO_EXT:biological_adhesion_or_attachedness"},{"id":"T4443","span":{"begin":130,"end":135},"obj":"CL_GO_EXT:cell"},{"id":"T4444","span":{"begin":212,"end":242},"obj":"CHEBI:28714"},{"id":"T4445","span":{"begin":244,"end":247},"obj":"CHEBI:28714"},{"id":"T4446","span":{"begin":260,"end":264},"obj":"CHEBI_EXT:23888"},{"id":"T4447","span":{"begin":279,"end":290},"obj":"CHEBI:16856"},{"id":"T4448","span":{"begin":279,"end":300},"obj":"GO:0006750"},{"id":"T4449","span":{"begin":314,"end":319},"obj":"CL_GO_EXT:cell"},{"id":"T4450","span":{"begin":354,"end":359},"obj":"CHEBI:35456"},{"id":"T4451","span":{"begin":458,"end":461},"obj":"CHEBI:53233"},{"id":"T4452","span":{"begin":463,"end":523},"obj":"CHEBI:53233"},{"id":"T4453","span":{"begin":531,"end":535},"obj":"CL_GO_EXT:cell"},{"id":"T4454","span":{"begin":531,"end":549},"obj":"GO:0008283"}],"text":"Cytotoxicity assay\nSamples of 1 × 104 cells/well were plated in 96-well tissue culture plates and allowed to adhere for 24 h. The cells were then pre-incubated for 24 h in medium containing 0, 5, 10, 25 or 50 µM l-buthionine-[S,R]-sulfoximine (BSO) (Sigma), a drug that inhibits glutathione synthesis (31). Later, cells were exposed to 0, 5, 10 or 20 µM CdCl2 in the specified pre-incubation medium for an additional 24 h. Cytotoxicity was determined by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromid)-based Cell Proliferation Kit I (Roche) according to the manufacturer's instructions."}