PMC:1253828 / 10378-10897 JSONTXT

Annnotations TAB JSON ListView MergeView

    craft-sa-dev

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nuclease mapping of transcripts (S1 analysis)\nS1 analysis was performed as previously described (29), using 100 µg DNase I-digested total RNA (RNA isolation see microarray analysis). The gels were developed using PhosphorImager (Molecular Dynamics). S1 analysis was done with the following 32P-labeled oligonucleotides: Hprt S1: 5′-TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG-3′, Sepw1 S1: 5′-TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC-3′."}

    2_test

    {"project":"2_test","denotations":[{"id":"16221973-390497-77403415","span":{"begin":100,"end":102},"obj":"390497"}],"text":"S1 nuclease mapping of transcripts (S1 analysis)\nS1 analysis was performed as previously described (29), using 100 µg DNase I-digested total RNA (RNA isolation see microarray analysis). The gels were developed using PhosphorImager (Molecular Dynamics). S1 analysis was done with the following 32P-labeled oligonucleotides: Hprt S1: 5′-TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG-3′, Sepw1 S1: 5′-TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC-3′."}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T3222","span":{"begin":0,"end":11},"obj":"PR:000025471"},{"id":"T3223","span":{"begin":23,"end":34},"obj":"SO:0000673"},{"id":"T3224","span":{"begin":36,"end":38},"obj":"PR:000025471"},{"id":"T3225","span":{"begin":49,"end":51},"obj":"PR:000025471"},{"id":"T3226","span":{"begin":253,"end":255},"obj":"PR:000025471"},{"id":"T3227","span":{"begin":293,"end":296},"obj":"CHEBI:37972"},{"id":"T3228","span":{"begin":328,"end":330},"obj":"PR:000025471"},{"id":"T3229","span":{"begin":412,"end":417},"obj":"PR:000014662"},{"id":"T3230","span":{"begin":418,"end":420},"obj":"PR:000025471"}],"text":"S1 nuclease mapping of transcripts (S1 analysis)\nS1 analysis was performed as previously described (29), using 100 µg DNase I-digested total RNA (RNA isolation see microarray analysis). The gels were developed using PhosphorImager (Molecular Dynamics). S1 analysis was done with the following 32P-labeled oligonucleotides: Hprt S1: 5′-TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG-3′, Sepw1 S1: 5′-TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC-3′."}

    craft-ca-core-ex-dev

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