PMC:1247560 / 7549-8751 JSONTXT

{"project":"2_test","denotations":[{"id":"15471725-10025415-28196395","span":{"begin":774,"end":778},"obj":"10025415"}],"text":"We determined the concentrations of p,p′-dichlorodiphenyl-dichloroethylene (DDE) and 14 PCB congeners (International Union of Pure and Applied Chemistry numbers 28, 52, 99, 101, 105, 118, 128, 138, 153, 156, 170, 180, 183, and 187) in plasma samples by high-resolution gas chromatography. OCs were extracted from plasma with ammonium sulfate:ethanol: hexane (1:1:3). The extracts were cleaned on florisil columns, taken to a final volume of 100 μL, and analyzed on an HP-5890 series II gas chromatograph equipped with dual-capillary columns and dual Ni-63 electron-capture detectors (Hewlett-Packard, Palo Alto, CA, USA). We identified peaks by their relative retention times obtained on the two columns. Quality control procedures were described previously (Rhainds et al. 1999). Percent recovery ranged from 89 to 100%, and the detection limit was approximately 0.02 μg/L for all compounds. Coefficients of variation (n = 20, different days) ranged from 2.1 to 9.1%. The difference between the concentration of reference material and that found using the analytic method ranged from 10.9 to 3.8%. Because OCs are stored mainly in body fat, all results for contaminants are expressed on a lipid basis."}

{"project":"MyTest","denotations":[{"id":"15471725-10025415-28196395","span":{"begin":774,"end":778},"obj":"10025415"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"We determined the concentrations of p,p′-dichlorodiphenyl-dichloroethylene (DDE) and 14 PCB congeners (International Union of Pure and Applied Chemistry numbers 28, 52, 99, 101, 105, 118, 128, 138, 153, 156, 170, 180, 183, and 187) in plasma samples by high-resolution gas chromatography. OCs were extracted from plasma with ammonium sulfate:ethanol: hexane (1:1:3). The extracts were cleaned on florisil columns, taken to a final volume of 100 μL, and analyzed on an HP-5890 series II gas chromatograph equipped with dual-capillary columns and dual Ni-63 electron-capture detectors (Hewlett-Packard, Palo Alto, CA, USA). We identified peaks by their relative retention times obtained on the two columns. Quality control procedures were described previously (Rhainds et al. 1999). Percent recovery ranged from 89 to 100%, and the detection limit was approximately 0.02 μg/L for all compounds. Coefficients of variation (n = 20, different days) ranged from 2.1 to 9.1%. The difference between the concentration of reference material and that found using the analytic method ranged from 10.9 to 3.8%. Because OCs are stored mainly in body fat, all results for contaminants are expressed on a lipid basis."}