Id |
Subject |
Object |
Predicate |
Lexical cue |
T2839 |
0-17 |
JJ |
denotes |
Semi-quantitative |
T2840 |
18-21 |
NN |
denotes |
PCR |
T2841 |
21-206 |
sentence |
denotes |
To check that the Homer3, Dncl1 and Trim11 clones were not identified multiple times solely because they are highly abundant in the library, we performed a semi-quantitative PCR assay. |
T2842 |
22-24 |
TO |
denotes |
To |
T2843 |
25-30 |
VB |
denotes |
check |
T2844 |
166-175 |
VBD |
denotes |
performed |
T2845 |
31-35 |
IN |
denotes |
that |
T2846 |
81-91 |
VBN |
denotes |
identified |
T2847 |
36-39 |
DT |
denotes |
the |
T2848 |
65-71 |
NNS |
denotes |
clones |
T2849 |
40-46 |
NN |
denotes |
Homer3 |
T2850 |
46-48 |
, |
denotes |
, |
T2851 |
48-53 |
NN |
denotes |
Dncl1 |
T2852 |
54-57 |
CC |
denotes |
and |
T2853 |
58-64 |
NN |
denotes |
Trim11 |
T2854 |
72-76 |
VBD |
denotes |
were |
T2855 |
77-80 |
RB |
denotes |
not |
T2856 |
92-100 |
JJ |
denotes |
multiple |
T2857 |
101-106 |
NNS |
denotes |
times |
T2858 |
107-113 |
RB |
denotes |
solely |
T2859 |
127-130 |
VBP |
denotes |
are |
T2860 |
114-121 |
IN |
denotes |
because |
T2861 |
122-126 |
PRP |
denotes |
they |
T2862 |
131-137 |
RB |
denotes |
highly |
T2863 |
138-146 |
JJ |
denotes |
abundant |
T2864 |
147-149 |
IN |
denotes |
in |
T2865 |
150-153 |
DT |
denotes |
the |
T2866 |
154-161 |
NN |
denotes |
library |
T2867 |
161-163 |
, |
denotes |
, |
T2868 |
163-165 |
PRP |
denotes |
we |
T2869 |
176-177 |
DT |
denotes |
a |
T2870 |
200-205 |
NN |
denotes |
assay |
T2871 |
178-195 |
JJ |
denotes |
semi-quantitative |
T2872 |
196-199 |
NN |
denotes |
PCR |
T2873 |
205-206 |
. |
denotes |
. |
T2874 |
206-298 |
sentence |
denotes |
We compared the relative abundance of Homer3, Dncl1, Trim11 and Pax6 with Gapdh and Atp5a1. |
T2875 |
207-209 |
PRP |
denotes |
We |
T2876 |
210-218 |
VBD |
denotes |
compared |
T2877 |
219-222 |
DT |
denotes |
the |
T2878 |
232-241 |
NN |
denotes |
abundance |
T2879 |
223-231 |
JJ |
denotes |
relative |
T2880 |
242-244 |
IN |
denotes |
of |
T2881 |
245-251 |
NN |
denotes |
Homer3 |
T2882 |
251-253 |
, |
denotes |
, |
T2883 |
253-258 |
NN |
denotes |
Dncl1 |
T2884 |
258-260 |
, |
denotes |
, |
T2885 |
260-266 |
NN |
denotes |
Trim11 |
T2886 |
267-270 |
CC |
denotes |
and |
T2887 |
271-275 |
NN |
denotes |
Pax6 |
T2888 |
276-280 |
IN |
denotes |
with |
T2889 |
281-286 |
NN |
denotes |
Gapdh |
T2890 |
287-290 |
CC |
denotes |
and |
T2891 |
291-297 |
NN |
denotes |
Atp5a1 |
T2892 |
297-298 |
. |
denotes |
. |
T2893 |
298-409 |
sentence |
denotes |
Gapdh and Atp5a1 both show strong constitutive expression in a variety of tissues including the brain [29,30]. |
T2894 |
299-304 |
NN |
denotes |
Gapdh |
T2895 |
321-325 |
VBP |
denotes |
show |
T2896 |
305-308 |
CC |
denotes |
and |
T2897 |
309-315 |
NN |
denotes |
Atp5a1 |
T2898 |
316-320 |
DT |
denotes |
both |
T2899 |
326-332 |
JJ |
denotes |
strong |
T2900 |
346-356 |
NN |
denotes |
expression |
T2901 |
333-345 |
JJ |
denotes |
constitutive |
T2902 |
357-359 |
IN |
denotes |
in |
T2903 |
360-361 |
DT |
denotes |
a |
T2904 |
362-369 |
NN |
denotes |
variety |
T2905 |
370-372 |
IN |
denotes |
of |
T2906 |
373-380 |
NNS |
denotes |
tissues |
T2907 |
381-390 |
VBG |
denotes |
including |
T2908 |
391-394 |
DT |
denotes |
the |
T2909 |
395-400 |
NN |
denotes |
brain |
T2910 |
401-402 |
-LRB- |
denotes |
[ |
T2911 |
405-407 |
CD |
denotes |
30 |
T2912 |
402-404 |
CD |
denotes |
29 |
T2913 |
404-405 |
, |
denotes |
, |
T2914 |
407-408 |
-RRB- |
denotes |
] |
T2915 |
408-409 |
. |
denotes |
. |
T2916 |
409-669 |
sentence |
denotes |
Homer3, Dncl1, Trim11 and Pax6 were only amplified strongly after 35 cycles of PCR (Figure 2) and were therefore present at relatively low levels compared to Gapdh (amplified strongly after 25 cycles) and Atp5a1 (amplified strongly after 30 cycles; Figure 2). |
T2917 |
410-416 |
NN |
denotes |
Homer3 |
T2918 |
451-460 |
VBN |
denotes |
amplified |
T2919 |
416-418 |
, |
denotes |
, |
T2920 |
418-423 |
NN |
denotes |
Dncl1 |
T2921 |
423-425 |
, |
denotes |
, |
T2922 |
425-431 |
NN |
denotes |
Trim11 |
T2923 |
432-435 |
CC |
denotes |
and |
T2924 |
436-440 |
NN |
denotes |
Pax6 |
T2925 |
441-445 |
VBD |
denotes |
were |
T2926 |
446-450 |
RB |
denotes |
only |
T2927 |
461-469 |
RB |
denotes |
strongly |
T2928 |
470-475 |
IN |
denotes |
after |
T2929 |
476-478 |
CD |
denotes |
35 |
T2930 |
479-485 |
NNS |
denotes |
cycles |
T2931 |
486-488 |
IN |
denotes |
of |
T2932 |
489-492 |
NN |
denotes |
PCR |
T2933 |
493-494 |
-LRB- |
denotes |
( |
T2934 |
494-500 |
NN |
denotes |
Figure |
T2935 |
501-502 |
CD |
denotes |
2 |
T2936 |
502-503 |
-RRB- |
denotes |
) |
T2937 |
504-507 |
CC |
denotes |
and |
T2938 |
508-512 |
VBD |
denotes |
were |
T2939 |
513-522 |
RB |
denotes |
therefore |
T2940 |
523-530 |
JJ |
denotes |
present |
T2941 |
531-533 |
IN |
denotes |
at |
T2942 |
534-544 |
RB |
denotes |
relatively |
T2943 |
545-548 |
JJ |
denotes |
low |
T2944 |
549-555 |
NNS |
denotes |
levels |
T2945 |
556-564 |
VBN |
denotes |
compared |
T3000 |
1309-1312 |
DT |
denotes |
the |
T3001 |
1321-1329 |
NN |
denotes |
proteins |
T3002 |
1313-1320 |
VBN |
denotes |
encoded |
T3003 |
1339-1351 |
RB |
denotes |
specifically |
T3004 |
1352-1356 |
IN |
denotes |
with |
T3005 |
1357-1360 |
DT |
denotes |
the |
T3006 |
1372-1379 |
NN |
denotes |
peptide |
T3007 |
1361-1362 |
NN |
denotes |
C |
T3008 |
1363-1371 |
JJ |
denotes |
terminal |
T3009 |
1362-1363 |
HYPH |
denotes |
- |
T3010 |
1380-1382 |
CC |
denotes |
or |
T3011 |
1383-1386 |
NN |
denotes |
PST |
T3012 |
1387-1393 |
NN |
denotes |
domain |
T3013 |
1394-1396 |
IN |
denotes |
of |
T3014 |
1397-1401 |
NN |
denotes |
PAX6 |
T3015 |
1401-1402 |
. |
denotes |
. |
T8468 |
680-697 |
JJ |
denotes |
Semi-quantitative |
T8469 |
702-710 |
NN |
denotes |
analysis |
T8470 |
698-701 |
NN |
denotes |
PCR |
T8471 |
711-713 |
IN |
denotes |
of |
T8472 |
714-720 |
NN |
denotes |
Homer3 |
T8473 |
720-722 |
, |
denotes |
, |
T8474 |
722-727 |
NN |
denotes |
Dncl1 |
T8475 |
727-729 |
, |
denotes |
, |
T8476 |
729-735 |
NN |
denotes |
Trim11 |
T8477 |
736-739 |
CC |
denotes |
and |
T8478 |
740-744 |
NN |
denotes |
Pax6 |
T8479 |
745-747 |
IN |
denotes |
in |
T8480 |
748-751 |
DT |
denotes |
the |
T8481 |
769-776 |
NN |
denotes |
library |
T8482 |
752-757 |
NN |
denotes |
mouse |
T8483 |
758-763 |
NN |
denotes |
brain |
T8484 |
764-768 |
NN |
denotes |
cDNA |
T8485 |
776-777 |
. |
denotes |
. |
T8486 |
777-931 |
sentence |
denotes |
Library cDNA was amplified with primers specific for Pax6 (600 bp), Homer3 (485 bp band), Dncl1 (485 bp) and Trim11 (609 bp) for 20, 25, 30 or 35 cycles. |
T8487 |
778-785 |
NN |
denotes |
Library |
T8488 |
786-790 |
NN |
denotes |
cDNA |
T8489 |
795-804 |
VBN |
denotes |
amplified |
T8490 |
791-794 |
VBD |
denotes |
was |
T8491 |
805-809 |
IN |
denotes |
with |
T8492 |
810-817 |
NNS |
denotes |
primers |
T8493 |
818-826 |
JJ |
denotes |
specific |
T8494 |
827-830 |
IN |
denotes |
for |
T8495 |
831-835 |
NN |
denotes |
Pax6 |
T8496 |
836-837 |
-LRB- |
denotes |
( |
T8497 |
841-843 |
NN |
denotes |
bp |
T8498 |
837-840 |
CD |
denotes |
600 |
T8499 |
843-844 |
-RRB- |
denotes |
) |
T8500 |
844-846 |
, |
denotes |
, |
T8501 |
846-852 |
NN |
denotes |
Homer3 |
T8502 |
853-854 |
-LRB- |
denotes |
( |
T8503 |
861-865 |
NN |
denotes |
band |
T8504 |
854-857 |
CD |
denotes |
485 |
T8505 |
858-860 |
NN |
denotes |
bp |
T8506 |
865-866 |
-RRB- |
denotes |
) |
T8507 |
866-868 |
, |
denotes |
, |
T8508 |
868-873 |
NN |
denotes |
Dncl1 |
T8509 |
874-875 |
-LRB- |
denotes |
( |
T8510 |
879-881 |
NN |
denotes |
bp |
T8511 |
875-878 |
CD |
denotes |
485 |
T8512 |
881-882 |
-RRB- |
denotes |
) |
T8513 |
883-886 |
CC |
denotes |
and |
T8514 |
887-893 |
NN |
denotes |
Trim11 |
T8515 |
894-895 |
-LRB- |
denotes |
( |
T8516 |
899-901 |
NN |
denotes |
bp |
T8517 |
895-898 |
CD |
denotes |
609 |
T8518 |
901-902 |
-RRB- |
denotes |
) |
T8519 |
903-906 |
IN |
denotes |
for |
T8520 |
907-909 |
CD |
denotes |
20 |
T8521 |
924-930 |
NNS |
denotes |
cycles |
T8522 |
909-911 |
, |
denotes |
, |
T8523 |
911-913 |
CD |
denotes |
25 |
T8524 |
913-915 |
, |
denotes |
, |
T8525 |
915-917 |
CD |
denotes |
30 |
T8526 |
918-920 |
CC |
denotes |
or |
T8527 |
921-923 |
CD |
denotes |
35 |
T8528 |
930-931 |
. |
denotes |
. |
T8529 |
931-1037 |
sentence |
denotes |
Atp5a1 (415 bp) and Gapdh (450 bp), which are highly expressed in the brain, are included for comparison. |
T8530 |
932-938 |
NN |
denotes |
Atp5a1 |
T8531 |
1013-1021 |
VBN |
denotes |
included |
T8532 |
939-940 |
-LRB- |
denotes |
( |
T8533 |
944-946 |
NN |
denotes |
bp |
T8534 |
940-943 |
CD |
denotes |
415 |
T8535 |
946-947 |
-RRB- |
denotes |
) |
T8536 |
948-951 |
CC |
denotes |
and |
T8537 |
952-957 |
NN |
denotes |
Gapdh |
T8538 |
958-959 |
-LRB- |
denotes |
( |
T8539 |
963-965 |
NN |
denotes |
bp |
T8540 |
959-962 |
CD |
denotes |
450 |
T8541 |
965-966 |
-RRB- |
denotes |
) |
T8542 |
966-968 |
, |
denotes |
, |
T8543 |
968-973 |
WDT |
denotes |
which |
T8544 |
985-994 |
VBN |
denotes |
expressed |
T8545 |
974-977 |
VBP |
denotes |
are |
T8546 |
978-984 |
RB |
denotes |
highly |
T8547 |
995-997 |
IN |
denotes |
in |
T8548 |
998-1001 |
DT |
denotes |
the |
T8549 |
1002-1007 |
NN |
denotes |
brain |
T8550 |
1007-1009 |
, |
denotes |
, |
T8551 |
1009-1012 |
VBP |
denotes |
are |
T8552 |
1022-1025 |
IN |
denotes |
for |
T8553 |
1026-1036 |
NN |
denotes |
comparison |
T8554 |
1036-1037 |
. |
denotes |
. |
T8555 |
1037-1150 |
sentence |
denotes |
M indicates the Φ×174 HaeIII DNA size marker; the positions of the 603 bp and 310 bp marker bands are indicated. |
T8556 |
1038-1039 |
NN |
denotes |
M |
T8557 |
1040-1049 |
VBZ |
denotes |
indicates |
T8558 |
1140-1149 |
VBN |
denotes |
indicated |
T8559 |
1050-1053 |
DT |
denotes |
the |
T8560 |
1076-1082 |
NN |
denotes |
marker |
T8561 |
1054-1056 |
NN |
denotes |
Φ× |
T8562 |
1060-1066 |
NN |
denotes |
HaeIII |
T8563 |
1056-1059 |
CD |
denotes |
174 |
T8564 |
1067-1070 |
NN |
denotes |
DNA |
T8565 |
1071-1075 |
NN |
denotes |
size |
T8566 |
1082-1083 |
: |
denotes |
; |
T8567 |
1084-1087 |
DT |
denotes |
the |
T8568 |
1088-1097 |
NNS |
denotes |
positions |
T8569 |
1098-1100 |
IN |
denotes |
of |
T8570 |
1101-1104 |
DT |
denotes |
the |
T8571 |
1130-1135 |
NNS |
denotes |
bands |
T8572 |
1105-1108 |
CD |
denotes |
603 |
T8573 |
1109-1111 |
NN |
denotes |
bp |
T8574 |
1112-1115 |
CC |
denotes |
and |
T8575 |
1116-1119 |
CD |
denotes |
310 |
T8576 |
1120-1122 |
NN |
denotes |
bp |
T8577 |
1123-1129 |
NN |
denotes |
marker |
T8578 |
1136-1139 |
VBP |
denotes |
are |
T8579 |
1149-1150 |
. |
denotes |
. |
T2946 |
565-567 |
IN |
denotes |
to |
T2947 |
568-573 |
NN |
denotes |
Gapdh |
T2948 |
574-575 |
-LRB- |
denotes |
( |
T2949 |
575-584 |
VBN |
denotes |
amplified |
T2950 |
585-593 |
RB |
denotes |
strongly |
T2951 |
594-599 |
IN |
denotes |
after |
T2952 |
600-602 |
CD |
denotes |
25 |
T2953 |
603-609 |
NNS |
denotes |
cycles |
T2954 |
609-610 |
-RRB- |
denotes |
) |
T2955 |
611-614 |
CC |
denotes |
and |
T2956 |
615-621 |
NN |
denotes |
Atp5a1 |
T2957 |
622-623 |
-LRB- |
denotes |
( |
T2958 |
623-632 |
VBN |
denotes |
amplified |
T2959 |
633-641 |
RB |
denotes |
strongly |
T2960 |
642-647 |
IN |
denotes |
after |
T2961 |
648-650 |
CD |
denotes |
30 |
T2962 |
651-657 |
NNS |
denotes |
cycles |
T2963 |
657-658 |
: |
denotes |
; |
T2964 |
659-665 |
NN |
denotes |
Figure |
T2965 |
666-667 |
CD |
denotes |
2 |
T2966 |
667-668 |
-RRB- |
denotes |
) |
T2967 |
668-669 |
. |
denotes |
. |
T2968 |
669-1241 |
sentence |
denotes |
Figure 2 Semi-quantitative PCR analysis of Homer3, Dncl1, Trim11 and Pax6 in the mouse brain cDNA library. Library cDNA was amplified with primers specific for Pax6 (600 bp), Homer3 (485 bp band), Dncl1 (485 bp) and Trim11 (609 bp) for 20, 25, 30 or 35 cycles. Atp5a1 (415 bp) and Gapdh (450 bp), which are highly expressed in the brain, are included for comparison. M indicates the Φ×174 HaeIII DNA size marker; the positions of the 603 bp and 310 bp marker bands are indicated. We concluded that Homer3, Dncl1 and Trim11 clones were not highly abundant in the library. |
T2969 |
1151-1153 |
PRP |
denotes |
We |
T2970 |
1154-1163 |
VBD |
denotes |
concluded |
T2971 |
1164-1168 |
IN |
denotes |
that |
T2972 |
1201-1205 |
VBD |
denotes |
were |
T2973 |
1169-1175 |
NN |
denotes |
Homer3 |
T2974 |
1194-1200 |
NNS |
denotes |
clones |
T2975 |
1175-1177 |
, |
denotes |
, |
T2976 |
1177-1182 |
NN |
denotes |
Dncl1 |
T2977 |
1183-1186 |
CC |
denotes |
and |
T2978 |
1187-1193 |
NN |
denotes |
Trim11 |
T2979 |
1206-1209 |
RB |
denotes |
not |
T2980 |
1210-1216 |
RB |
denotes |
highly |
T2981 |
1217-1225 |
JJ |
denotes |
abundant |
T2982 |
1226-1228 |
IN |
denotes |
in |
T2983 |
1229-1232 |
DT |
denotes |
the |
T2984 |
1233-1240 |
NN |
denotes |
library |
T2985 |
1240-1241 |
. |
denotes |
. |
T2986 |
1241-1402 |
sentence |
denotes |
This is consistent with the idea that they were pulled out because the encoded proteins interact specifically with the C-terminal peptide or PST domain of PAX6. |
T2987 |
1242-1246 |
DT |
denotes |
This |
T2988 |
1247-1249 |
VBZ |
denotes |
is |
T2989 |
1250-1260 |
JJ |
denotes |
consistent |
T2990 |
1261-1265 |
IN |
denotes |
with |
T2991 |
1266-1269 |
DT |
denotes |
the |
T2992 |
1270-1274 |
NN |
denotes |
idea |
T2993 |
1275-1279 |
IN |
denotes |
that |
T2994 |
1290-1296 |
VBN |
denotes |
pulled |
T2995 |
1280-1284 |
PRP |
denotes |
they |
T2996 |
1285-1289 |
VBD |
denotes |
were |
T2997 |
1297-1300 |
RP |
denotes |
out |
T2998 |
1301-1308 |
IN |
denotes |
because |
T2999 |
1330-1338 |
VBP |
denotes |
interact |
R1747 |
T2839 |
T2840 |
amod |
Semi-quantitative,PCR |
R1748 |
T2842 |
T2843 |
aux |
To,check |
R1749 |
T2843 |
T2844 |
advcl |
check,performed |
R1750 |
T2845 |
T2846 |
mark |
that,identified |
R1751 |
T2846 |
T2843 |
ccomp |
identified,check |
R1752 |
T2847 |
T2848 |
det |
the,clones |
R1753 |
T2848 |
T2846 |
nsubjpass |
clones,identified |
R1754 |
T2849 |
T2848 |
nmod |
Homer3,clones |
R1755 |
T2850 |
T2849 |
punct |
", ",Homer3 |
R1756 |
T2851 |
T2849 |
conj |
Dncl1,Homer3 |
R1757 |
T2852 |
T2851 |
cc |
and,Dncl1 |
R1758 |
T2853 |
T2851 |
conj |
Trim11,Dncl1 |
R1759 |
T2854 |
T2846 |
auxpass |
were,identified |
R1760 |
T2855 |
T2846 |
neg |
not,identified |
R1761 |
T2856 |
T2857 |
amod |
multiple,times |
R1762 |
T2857 |
T2846 |
npadvmod |
times,identified |
R1763 |
T2858 |
T2859 |
advmod |
solely,are |
R1764 |
T2859 |
T2846 |
advcl |
are,identified |
R1765 |
T2860 |
T2859 |
mark |
because,are |
R1766 |
T2861 |
T2859 |
nsubj |
they,are |
R1767 |
T2862 |
T2863 |
advmod |
highly,abundant |
R1768 |
T2863 |
T2859 |
acomp |
abundant,are |
R1769 |
T2864 |
T2859 |
prep |
in,are |
R1770 |
T2865 |
T2866 |
det |
the,library |
R1771 |
T2866 |
T2864 |
pobj |
library,in |
R1772 |
T2867 |
T2844 |
punct |
", ",performed |
R1773 |
T2868 |
T2844 |
nsubj |
we,performed |
R1774 |
T2869 |
T2870 |
det |
a,assay |
R1775 |
T2870 |
T2844 |
dobj |
assay,performed |
R1776 |
T2871 |
T2870 |
amod |
semi-quantitative,assay |
R1777 |
T2872 |
T2870 |
compound |
PCR,assay |
R1778 |
T2873 |
T2844 |
punct |
.,performed |
R1779 |
T2875 |
T2876 |
nsubj |
We,compared |
R1780 |
T2877 |
T2878 |
det |
the,abundance |
R1781 |
T2878 |
T2876 |
dobj |
abundance,compared |
R1782 |
T2879 |
T2878 |
amod |
relative,abundance |
R1783 |
T2880 |
T2878 |
prep |
of,abundance |
R1784 |
T2881 |
T2880 |
pobj |
Homer3,of |
R1785 |
T2882 |
T2881 |
punct |
", ",Homer3 |
R1786 |
T2883 |
T2881 |
conj |
Dncl1,Homer3 |
R1787 |
T2884 |
T2883 |
punct |
", ",Dncl1 |
R1788 |
T2885 |
T2883 |
conj |
Trim11,Dncl1 |
R1789 |
T2886 |
T2885 |
cc |
and,Trim11 |
R1790 |
T2887 |
T2885 |
conj |
Pax6,Trim11 |
R1791 |
T2888 |
T2876 |
prep |
with,compared |
R1792 |
T2889 |
T2888 |
pobj |
Gapdh,with |
R1793 |
T2890 |
T2889 |
cc |
and,Gapdh |
R1794 |
T2891 |
T2889 |
conj |
Atp5a1,Gapdh |
R1795 |
T2892 |
T2876 |
punct |
.,compared |
R1796 |
T2894 |
T2895 |
nsubj |
Gapdh,show |
R1797 |
T2896 |
T2894 |
cc |
and,Gapdh |
R1798 |
T2897 |
T2894 |
conj |
Atp5a1,Gapdh |
R1799 |
T2898 |
T2894 |
appos |
both,Gapdh |
R1800 |
T2899 |
T2900 |
amod |
strong,expression |
R1801 |
T2900 |
T2895 |
dobj |
expression,show |
R1802 |
T2901 |
T2900 |
amod |
constitutive,expression |
R1803 |
T2902 |
T2895 |
prep |
in,show |
R1804 |
T2903 |
T2904 |
det |
a,variety |
R1805 |
T2904 |
T2902 |
pobj |
variety,in |
R1806 |
T2905 |
T2904 |
prep |
of,variety |
R1807 |
T2906 |
T2905 |
pobj |
tissues,of |
R1808 |
T2907 |
T2906 |
prep |
including,tissues |
R1809 |
T2908 |
T2909 |
det |
the,brain |
R1810 |
T2909 |
T2907 |
pobj |
brain,including |
R1811 |
T2910 |
T2911 |
punct |
[,30 |
R1812 |
T2911 |
T2895 |
parataxis |
30,show |
R1813 |
T2912 |
T2911 |
nummod |
29,30 |
R1814 |
T2913 |
T2911 |
punct |
",",30 |
R1844 |
T2945 |
T2938 |
prep |
compared,were |
R1845 |
T2946 |
T2945 |
prep |
to,compared |
R1846 |
T2947 |
T2946 |
pobj |
Gapdh,to |
R1847 |
T2948 |
T2947 |
punct |
(,Gapdh |
R1848 |
T2949 |
T2947 |
acl |
amplified,Gapdh |
R1849 |
T2950 |
T2949 |
advmod |
strongly,amplified |
R1850 |
T2951 |
T2949 |
prep |
after,amplified |
R1851 |
T2952 |
T2953 |
nummod |
25,cycles |
R1852 |
T2953 |
T2951 |
pobj |
cycles,after |
R1853 |
T2954 |
T2947 |
punct |
),Gapdh |
R1854 |
T2955 |
T2947 |
cc |
and,Gapdh |
R1855 |
T2956 |
T2947 |
conj |
Atp5a1,Gapdh |
R1856 |
T2957 |
T2956 |
punct |
(,Atp5a1 |
R1857 |
T2958 |
T2956 |
acl |
amplified,Atp5a1 |
R1858 |
T2959 |
T2958 |
advmod |
strongly,amplified |
R1859 |
T2960 |
T2958 |
prep |
after,amplified |
R1860 |
T2961 |
T2962 |
nummod |
30,cycles |
R1861 |
T2962 |
T2960 |
pobj |
cycles,after |
R1862 |
T2963 |
T2964 |
punct |
;,Figure |
R1863 |
T2964 |
T2938 |
parataxis |
Figure,were |
R1864 |
T2965 |
T2964 |
nummod |
2,Figure |
R1865 |
T2966 |
T2964 |
punct |
),Figure |
R1866 |
T2967 |
T2918 |
punct |
.,amplified |
R1869 |
T2972 |
T2970 |
ccomp |
were,concluded |
R1871 |
T2974 |
T2972 |
nsubj |
clones,were |
R1873 |
T2976 |
T2973 |
conj |
Dncl1,Homer3 |
R1874 |
T2977 |
T2976 |
cc |
and,Dncl1 |
R1875 |
T2978 |
T2976 |
conj |
Trim11,Dncl1 |
R1876 |
T2979 |
T2972 |
neg |
not,were |
R1877 |
T2980 |
T2981 |
advmod |
highly,abundant |
R1878 |
T2981 |
T2972 |
acomp |
abundant,were |
R1879 |
T2982 |
T2972 |
prep |
in,were |
R1880 |
T2983 |
T2984 |
det |
the,library |
R1881 |
T2984 |
T2982 |
pobj |
library,in |
R1882 |
T2985 |
T2970 |
punct |
.,concluded |
R1883 |
T2987 |
T2988 |
nsubj |
This,is |
R1884 |
T2989 |
T2988 |
acomp |
consistent,is |
R1885 |
T2990 |
T2989 |
prep |
with,consistent |
R1886 |
T2991 |
T2992 |
det |
the,idea |
R1887 |
T2992 |
T2990 |
pobj |
idea,with |
R1888 |
T2993 |
T2994 |
mark |
that,pulled |
R1889 |
T2994 |
T2992 |
acl |
pulled,idea |
R1890 |
T2995 |
T2994 |
nsubjpass |
they,pulled |
R1891 |
T2996 |
T2994 |
auxpass |
were,pulled |
R1892 |
T2997 |
T2994 |
prt |
out,pulled |
R1893 |
T2998 |
T2999 |
mark |
because,interact |
R1894 |
T2999 |
T2994 |
advcl |
interact,pulled |
R1895 |
T3000 |
T3001 |
det |
the,proteins |
R1896 |
T3001 |
T2999 |
nsubj |
proteins,interact |
R1897 |
T3002 |
T3001 |
amod |
encoded,proteins |
R1898 |
T3003 |
T2999 |
advmod |
specifically,interact |
R1899 |
T3004 |
T2999 |
prep |
with,interact |
R1900 |
T3005 |
T3006 |
det |
the,peptide |
R1901 |
T3006 |
T3004 |
pobj |
peptide,with |
R1902 |
T3007 |
T3008 |
npadvmod |
C,terminal |
R1903 |
T3008 |
T3006 |
amod |
terminal,peptide |
R1904 |
T3009 |
T3008 |
punct |
-,terminal |
R1905 |
T3010 |
T3006 |
cc |
or,peptide |
R1906 |
T3011 |
T3012 |
compound |
PST,domain |
R1907 |
T3012 |
T3006 |
conj |
domain,peptide |
R1908 |
T3013 |
T3012 |
prep |
of,domain |
R1909 |
T3014 |
T3013 |
pobj |
PAX6,of |
R1910 |
T3015 |
T2988 |
punct |
.,is |
R5394 |
T8468 |
T8469 |
amod |
Semi-quantitative,analysis |
R5395 |
T8470 |
T8469 |
compound |
PCR,analysis |
R5396 |
T8471 |
T8469 |
prep |
of,analysis |
R5397 |
T8472 |
T8471 |
pobj |
Homer3,of |
R5398 |
T8473 |
T8472 |
punct |
", ",Homer3 |
R5399 |
T8474 |
T8472 |
conj |
Dncl1,Homer3 |
R5400 |
T8475 |
T8474 |
punct |
", ",Dncl1 |
R5401 |
T8476 |
T8474 |
conj |
Trim11,Dncl1 |
R5402 |
T8477 |
T8476 |
cc |
and,Trim11 |
R5403 |
T8478 |
T8476 |
conj |
Pax6,Trim11 |
R5404 |
T8479 |
T8469 |
prep |
in,analysis |
R5405 |
T8480 |
T8481 |
det |
the,library |
R5406 |
T8481 |
T8479 |
pobj |
library,in |
R5407 |
T8482 |
T8483 |
compound |
mouse,brain |
R5408 |
T8483 |
T8481 |
compound |
brain,library |
R5409 |
T8484 |
T8481 |
compound |
cDNA,library |
R5410 |
T8485 |
T8469 |
punct |
.,analysis |
R5411 |
T8487 |
T8488 |
compound |
Library,cDNA |
R5412 |
T8488 |
T8489 |
nsubjpass |
cDNA,amplified |
R5413 |
T8490 |
T8489 |
auxpass |
was,amplified |
R5414 |
T8491 |
T8489 |
prep |
with,amplified |
R5415 |
T8492 |
T8491 |
pobj |
primers,with |
R5416 |
T8493 |
T8492 |
amod |
specific,primers |
R5417 |
T8494 |
T8493 |
prep |
for,specific |
R5418 |
T8495 |
T8494 |
pobj |
Pax6,for |
R5419 |
T8496 |
T8497 |
punct |
(,bp |
R5420 |
T8497 |
T8495 |
parataxis |
bp,Pax6 |
R5421 |
T8498 |
T8497 |
nummod |
600,bp |
R5422 |
T8499 |
T8497 |
punct |
),bp |
R5423 |
T8500 |
T8495 |
punct |
", ",Pax6 |
R5424 |
T8501 |
T8495 |
conj |
Homer3,Pax6 |
R5425 |
T8502 |
T8503 |
punct |
(,band |
R5426 |
T8503 |
T8501 |
parataxis |
band,Homer3 |
R5427 |
T8504 |
T8505 |
nummod |
485,bp |
R5428 |
T8505 |
T8503 |
compound |
bp,band |
R5429 |
T8506 |
T8503 |
punct |
),band |
R5430 |
T8507 |
T8501 |
punct |
", ",Homer3 |
R5431 |
T8508 |
T8501 |
conj |
Dncl1,Homer3 |
R5432 |
T8509 |
T8510 |
punct |
(,bp |
R5433 |
T8510 |
T8508 |
parataxis |
bp,Dncl1 |
R5434 |
T8511 |
T8510 |
nummod |
485,bp |
R5435 |
T8512 |
T8510 |
punct |
),bp |
R5436 |
T8513 |
T8508 |
cc |
and,Dncl1 |
R5437 |
T8514 |
T8508 |
conj |
Trim11,Dncl1 |
R5438 |
T8515 |
T8516 |
punct |
(,bp |
R5439 |
T8516 |
T8514 |
parataxis |
bp,Trim11 |
R5440 |
T8517 |
T8516 |
nummod |
609,bp |
R5441 |
T8518 |
T8516 |
punct |
),bp |
R5442 |
T8519 |
T8489 |
prep |
for,amplified |
R5443 |
T8520 |
T8521 |
nummod |
20,cycles |
R5444 |
T8521 |
T8519 |
pobj |
cycles,for |
R5445 |
T8522 |
T8520 |
punct |
", ",20 |
R5446 |
T8523 |
T8520 |
conj |
25,20 |
R5447 |
T8524 |
T8523 |
punct |
", ",25 |
R5448 |
T8525 |
T8523 |
conj |
30,25 |
R5449 |
T8526 |
T8525 |
cc |
or,30 |
R5450 |
T8527 |
T8525 |
conj |
35,30 |
R5451 |
T8528 |
T8489 |
punct |
.,amplified |
R5452 |
T8530 |
T8531 |
nsubjpass |
Atp5a1,included |
R5453 |
T8532 |
T8533 |
punct |
(,bp |
R5454 |
T8533 |
T8530 |
parataxis |
bp,Atp5a1 |
R5455 |
T8534 |
T8533 |
nummod |
415,bp |
R5456 |
T8535 |
T8533 |
punct |
),bp |
R5457 |
T8536 |
T8530 |
cc |
and,Atp5a1 |
R5458 |
T8537 |
T8530 |
conj |
Gapdh,Atp5a1 |
R5459 |
T8538 |
T8539 |
punct |
(,bp |
R5460 |
T8539 |
T8537 |
parataxis |
bp,Gapdh |
R5461 |
T8540 |
T8539 |
nummod |
450,bp |
R5462 |
T8541 |
T8539 |
punct |
),bp |
R5463 |
T8542 |
T8530 |
punct |
", ",Atp5a1 |
R5464 |
T8543 |
T8544 |
dep |
which,expressed |
R5465 |
T8544 |
T8530 |
relcl |
expressed,Atp5a1 |
R5466 |
T8545 |
T8544 |
auxpass |
are,expressed |
R5467 |
T8546 |
T8544 |
advmod |
highly,expressed |
R5468 |
T8547 |
T8544 |
prep |
in,expressed |
R5469 |
T8548 |
T8549 |
det |
the,brain |
R5470 |
T8549 |
T8547 |
pobj |
brain,in |
R5471 |
T8550 |
T8531 |
punct |
", ",included |
R5472 |
T8551 |
T8531 |
auxpass |
are,included |
R5473 |
T8552 |
T8531 |
prep |
for,included |
R5474 |
T8553 |
T8552 |
pobj |
comparison,for |
R5475 |
T8554 |
T8531 |
punct |
.,included |
R5476 |
T8556 |
T8557 |
nsubj |
M,indicates |
R5477 |
T8557 |
T8558 |
ccomp |
indicates,indicated |
R5478 |
T8559 |
T8560 |
det |
the,marker |
R5479 |
T8560 |
T8557 |
dobj |
marker,indicates |
R5480 |
T8561 |
T8562 |
nmod |
Φ×,HaeIII |
R5481 |
T8562 |
T8560 |
compound |
HaeIII,marker |
R5482 |
T8563 |
T8562 |
nummod |
174,HaeIII |
R5483 |
T8564 |
T8565 |
compound |
DNA,size |
R5484 |
T8565 |
T8560 |
compound |
size,marker |
R5485 |
T8566 |
T8558 |
punct |
;,indicated |
R5486 |
T8567 |
T8568 |
det |
the,positions |
R5487 |
T8568 |
T8558 |
nsubjpass |
positions,indicated |
R5488 |
T8569 |
T8568 |
prep |
of,positions |
R5489 |
T8570 |
T8571 |
det |
the,bands |
R5490 |
T8571 |
T8569 |
pobj |
bands,of |
R5491 |
T8572 |
T8573 |
nummod |
603,bp |
R5492 |
T8573 |
T8571 |
nmod |
bp,bands |
R5493 |
T8574 |
T8573 |
cc |
and,bp |
R5494 |
T8575 |
T8576 |
nummod |
310,bp |
R5495 |
T8576 |
T8573 |
conj |
bp,bp |
R5496 |
T8577 |
T8571 |
compound |
marker,bands |
R5497 |
T8578 |
T8558 |
auxpass |
are,indicated |
R5498 |
T8579 |
T8558 |
punct |
.,indicated |
R1815 |
T2914 |
T2911 |
punct |
],30 |
R1816 |
T2915 |
T2895 |
punct |
.,show |
R1817 |
T2917 |
T2918 |
nsubjpass |
Homer3,amplified |
R1818 |
T2919 |
T2917 |
punct |
", ",Homer3 |
R1819 |
T2920 |
T2917 |
conj |
Dncl1,Homer3 |
R1820 |
T2921 |
T2920 |
punct |
", ",Dncl1 |
R1821 |
T2922 |
T2920 |
conj |
Trim11,Dncl1 |
R1822 |
T2923 |
T2922 |
cc |
and,Trim11 |
R1823 |
T2924 |
T2922 |
conj |
Pax6,Trim11 |
R1824 |
T2925 |
T2918 |
auxpass |
were,amplified |
R1825 |
T2926 |
T2918 |
advmod |
only,amplified |
R1826 |
T2927 |
T2918 |
advmod |
strongly,amplified |
R1827 |
T2928 |
T2918 |
prep |
after,amplified |
R1828 |
T2929 |
T2930 |
nummod |
35,cycles |
R1829 |
T2930 |
T2928 |
pobj |
cycles,after |
R1830 |
T2931 |
T2930 |
prep |
of,cycles |
R1831 |
T2932 |
T2931 |
pobj |
PCR,of |
R1832 |
T2933 |
T2934 |
punct |
(,Figure |
R1833 |
T2934 |
T2918 |
parataxis |
Figure,amplified |
R1834 |
T2935 |
T2934 |
nummod |
2,Figure |
R1835 |
T2936 |
T2934 |
punct |
),Figure |
R1836 |
T2937 |
T2918 |
cc |
and,amplified |
R1837 |
T2938 |
T2918 |
conj |
were,amplified |
R1838 |
T2939 |
T2938 |
advmod |
therefore,were |
R1839 |
T2940 |
T2938 |
acomp |
present,were |
R1840 |
T2941 |
T2940 |
prep |
at,present |
R1841 |
T2942 |
T2943 |
advmod |
relatively,low |
R1842 |
T2943 |
T2944 |
amod |
low,levels |
R1843 |
T2944 |
T2941 |
pobj |
levels,at |
R1867 |
T2969 |
T2970 |
nsubj |
We,concluded |
R1868 |
T2971 |
T2972 |
mark |
that,were |
R1870 |
T2973 |
T2974 |
nmod |
Homer3,clones |
R1872 |
T2975 |
T2973 |
punct |
", ",Homer3 |