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C-terminal peptide screen gave 15 colonies that were positive with all three reporters and the PST domain screen gave 62 colonies. The interacting plasmids were isolated and the cDNA inserts sequenced. Three cDNAs were identified 3 or more times, Homer3 (NM_011984), Dncl1 (Dynein cytoplasmic light chain 1, NM_019682, also known as Pin or Dlc8) and Trim11 (Tripartite motif protein family member 11, NM_053168). Homer3 (6 clones) and Dncl1 (2 clones) were identified in the C-terminal peptide screen. Homer3 (7 clones), Dncl1 (2 clones) and Trim11 (6 clones) were identified in the PST domain screen. All cDNA inserts were in-frame with the coding region of the pPC86 GAL4 activation domain. None of the cDNAs was present in a list of known false positives [34]."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T2277","span":{"begin":4,"end":14},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T2278","span":{"begin":15,"end":22},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T2279","span":{"begin":103,"end":109},"obj":"SO_EXT:0000417"},{"id":"T2280","span":{"begin":151,"end":159},"obj":"SO_EXT:0000155"},{"id":"T2281","span":{"begin":182,"end":186},"obj":"SO_EXT:cDNA"},{"id":"T2282","span":{"begin":187,"end":194},"obj":"SO_EXT:0000667"},{"id":"T2283","span":{"begin":212,"end":217},"obj":"SO_EXT:cDNA"},{"id":"T2284","span":{"begin":251,"end":257},"obj":"PR_EXT:000008680"},{"id":"T2285","span":{"begin":271,"end":276},"obj":"PR_EXT:000002325"},{"id":"T2286","span":{"begin":278,"end":284},"obj":"GO:0030286"},{"id":"T2287","span":{"begin":278,"end":310},"obj":"PR_EXT:000002325"},{"id":"T2288","span":{"begin":285,"end":296},"obj":"GO:0005737"},{"id":"T2289","span":{"begin":337,"end":340},"obj":"PR_EXT:000002325"},{"id":"T2290","span":{"begin":344,"end":348},"obj":"PR_EXT:000002325"},{"id":"T2291","span":{"begin":354,"end":360},"obj":"PR_EXT:000016642"},{"id":"T2292","span":{"begin":362,"end":403},"obj":"PR_EXT:000016642"},{"id":"T2293","span":{"begin":373,"end":378},"obj":"SO_EXT:sequence_or_structure_motif"},{"id":"T2294","span":{"begin":379,"end":386},"obj":"CHEBI_PR_EXT:protein"},{"id":"T2295","span":{"begin":417,"end":423},"obj":"PR_EXT:000008680"},{"id":"T2296","span":{"begin":427,"end":433},"obj":"SO_EXT:sequence_cloned_entity"},{"id":"T2297","span":{"begin":439,"end":444},"obj":"PR_EXT:000002325"},{"id":"T2298","span":{"begin":448,"end":454},"obj":"SO_EXT:sequence_cloned_entity"},{"id":"T2299","span":{"begin":479,"end":489},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T2300","span":{"begin":490,"end":497},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T2301","span":{"begin":506,"end":512},"obj":"PR_EXT:000008680"},{"id":"T2302","span":{"begin":516,"end":522},"obj":"SO_EXT:sequence_cloned_entity"},{"id":"T2303","span":{"begin":525,"end":530},"obj":"PR_EXT:000002325"},{"id":"T2304","span":{"begin":534,"end":540},"obj":"SO_EXT:sequence_cloned_entity"},{"id":"T2305","span":{"begin":546,"end":552},"obj":"PR_EXT:000016642"},{"id":"T2306","span":{"begin":556,"end":562},"obj":"SO_EXT:sequence_cloned_entity"},{"id":"T2307","span":{"begin":591,"end":597},"obj":"SO_EXT:0000417"},{"id":"T2308","span":{"begin":610,"end":614},"obj":"SO_EXT:cDNA"},{"id":"T2309","span":{"begin":615,"end":622},"obj":"SO_EXT:0000667"},{"id":"T2310","span":{"begin":628,"end":636},"obj":"SO:0001817"},{"id":"T2311","span":{"begin":646,"end":659},"obj":"SO_EXT:coding_sequence"},{"id":"T2312","span":{"begin":673,"end":677},"obj":"PR_EXT:P04386"},{"id":"T2313","span":{"begin":689,"end":695},"obj":"SO_EXT:0000417"},{"id":"T2314","span":{"begin":709,"end":714},"obj":"SO_EXT:cDNA"}],"text":"The C-terminal peptide screen gave 15 colonies that were positive with all three reporters and the PST domain screen gave 62 colonies. The interacting plasmids were isolated and the cDNA inserts sequenced. Three cDNAs were identified 3 or more times, Homer3 (NM_011984), Dncl1 (Dynein cytoplasmic light chain 1, NM_019682, also known as Pin or Dlc8) and Trim11 (Tripartite motif protein family member 11, NM_053168). Homer3 (6 clones) and Dncl1 (2 clones) were identified in the C-terminal peptide screen. Homer3 (7 clones), Dncl1 (2 clones) and Trim11 (6 clones) were identified in the PST domain screen. All cDNA inserts were in-frame with the coding region of the pPC86 GAL4 activation domain. None of the cDNAs was present in a list of known false positives [34]."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T2205","span":{"begin":103,"end":109},"obj":"SO:0000417"},{"id":"T2206","span":{"begin":151,"end":159},"obj":"SO:0000155"},{"id":"T2207","span":{"begin":187,"end":194},"obj":"SO:0000667"},{"id":"T2208","span":{"begin":251,"end":257},"obj":"PR:000008680"},{"id":"T2209","span":{"begin":271,"end":276},"obj":"PR:000002325"},{"id":"T2210","span":{"begin":278,"end":284},"obj":"GO:0030286"},{"id":"T2211","span":{"begin":278,"end":310},"obj":"PR:000002325"},{"id":"T2212","span":{"begin":285,"end":296},"obj":"GO:0005737"},{"id":"T2213","span":{"begin":337,"end":340},"obj":"PR:000002325"},{"id":"T2214","span":{"begin":344,"end":348},"obj":"PR:000002325"},{"id":"T2215","span":{"begin":354,"end":360},"obj":"PR:000016642"},{"id":"T2216","span":{"begin":362,"end":403},"obj":"PR:000016642"},{"id":"T2217","span":{"begin":417,"end":423},"obj":"PR:000008680"},{"id":"T2218","span":{"begin":439,"end":444},"obj":"PR:000002325"},{"id":"T2219","span":{"begin":506,"end":512},"obj":"PR:000008680"},{"id":"T2220","span":{"begin":525,"end":530},"obj":"PR:000002325"},{"id":"T2221","span":{"begin":546,"end":552},"obj":"PR:000016642"},{"id":"T2222","span":{"begin":591,"end":597},"obj":"SO:0000417"},{"id":"T2223","span":{"begin":615,"end":622},"obj":"SO:0000667"},{"id":"T2224","span":{"begin":628,"end":636},"obj":"SO:0001817"},{"id":"T2225","span":{"begin":673,"end":677},"obj":"PR:P04386"},{"id":"T2226","span":{"begin":689,"end":695},"obj":"SO:0000417"}],"text":"The C-terminal peptide screen gave 15 colonies that were positive with all three reporters and the PST domain screen gave 62 colonies. The interacting plasmids were isolated and the cDNA inserts sequenced. Three cDNAs were identified 3 or more times, Homer3 (NM_011984), Dncl1 (Dynein cytoplasmic light chain 1, NM_019682, also known as Pin or Dlc8) and Trim11 (Tripartite motif protein family member 11, NM_053168). Homer3 (6 clones) and Dncl1 (2 clones) were identified in the C-terminal peptide screen. Homer3 (7 clones), Dncl1 (2 clones) and Trim11 (6 clones) were identified in the PST domain screen. All cDNA inserts were in-frame with the coding region of the pPC86 GAL4 activation domain. None of the cDNAs was present in a list of known false positives [34]."}