Id |
Subject |
Object |
Predicate |
Lexical cue |
T7663 |
528-529 |
. |
denotes |
. |
T7662 |
527-528 |
-RRB- |
denotes |
] |
T7661 |
525-527 |
CD |
denotes |
24 |
T7660 |
524-525 |
-LRB- |
denotes |
[ |
T7659 |
512-516 |
NN |
denotes |
cDNA |
T7658 |
517-523 |
NN |
denotes |
insert |
T7657 |
508-511 |
DT |
denotes |
the |
T7656 |
499-507 |
VB |
denotes |
identify |
T7655 |
496-498 |
TO |
denotes |
to |
T7654 |
481-485 |
VBD |
denotes |
were |
T7653 |
486-495 |
VBN |
denotes |
performed |
T7652 |
472-480 |
NNS |
denotes |
searches |
T7651 |
466-471 |
NNP |
denotes |
BLAST |
T7650 |
465-529 |
sentence |
denotes |
BLAST searches were performed to identify the cDNA insert [24]. |
T7649 |
464-465 |
. |
denotes |
. |
T7648 |
455-464 |
VBN |
denotes |
sequenced |
T7647 |
443-447 |
NN |
denotes |
cDNA |
T7646 |
448-454 |
NN |
denotes |
insert |
T7645 |
439-442 |
DT |
denotes |
the |
T7644 |
435-438 |
CC |
denotes |
and |
T7643 |
419-420 |
HYPH |
denotes |
/ |
T7642 |
415-419 |
NN |
denotes |
URA3 |
T7641 |
414-415 |
HYPH |
denotes |
/ |
T7640 |
420-424 |
NN |
denotes |
LacZ |
T7639 |
410-414 |
NN |
denotes |
HIS3 |
T7638 |
425-434 |
NNS |
denotes |
positives |
T7637 |
406-409 |
DT |
denotes |
all |
T7636 |
401-405 |
IN |
denotes |
from |
T7635 |
388-391 |
VBD |
denotes |
was |
T7634 |
392-400 |
VBN |
denotes |
isolated |
T7633 |
375-379 |
NN |
denotes |
prey |
T7632 |
369-374 |
NN |
denotes |
pPC86 |
T7631 |
380-387 |
NN |
denotes |
plasmid |
T7630 |
365-368 |
DT |
denotes |
The |
T7629 |
364-465 |
sentence |
denotes |
The pPC86 prey plasmid was isolated from all HIS3/URA3/LacZ positives and the cDNA insert sequenced. |
T7628 |
363-364 |
. |
denotes |
. |
T7627 |
352-353 |
CD |
denotes |
3 |
T7626 |
354-363 |
NNS |
denotes |
reporters |
T7625 |
348-351 |
DT |
denotes |
all |
T7624 |
344-347 |
IN |
denotes |
for |
T7623 |
331-335 |
RB |
denotes |
then |
T7622 |
326-330 |
VBD |
denotes |
were |
T7621 |
336-343 |
VBN |
denotes |
assayed |
T7620 |
316-325 |
NNS |
denotes |
positives |
T7619 |
311-315 |
NN |
denotes |
HIS3 |
T7618 |
310-364 |
sentence |
denotes |
HIS3 positives were then assayed for all 3 reporters. |
T7617 |
309-310 |
. |
denotes |
. |
T7616 |
299-309 |
NN |
denotes |
activation |
T7615 |
294-298 |
NN |
denotes |
HIS3 |
T7614 |
290-293 |
IN |
denotes |
for |
T7613 |
284-289 |
VB |
denotes |
check |
T7612 |
281-283 |
TO |
denotes |
to |
T7611 |
271-280 |
NN |
denotes |
histidine |
T7610 |
263-270 |
VBG |
denotes |
lacking |
T7609 |
256-262 |
NN |
denotes |
medium |
T7608 |
253-255 |
IN |
denotes |
on |
T7607 |
241-245 |
VBD |
denotes |
were |
T7606 |
239-240 |
-RRB- |
denotes |
) |
T7605 |
234-235 |
SYM |
denotes |
× |
T7604 |
232-233 |
CD |
denotes |
5 |
T7603 |
236-239 |
CD |
denotes |
107 |
T7602 |
231-232 |
-LRB- |
denotes |
( |
T7601 |
246-252 |
VBN |
denotes |
plated |
T7600 |
217-230 |
NNS |
denotes |
Transformants |
T7599 |
216-310 |
sentence |
denotes |
Transformants (5 × 107) were plated on medium lacking histidine to check for HIS3 activation. |
T7598 |
215-216 |
. |
denotes |
. |
T7597 |
208-215 |
NN |
denotes |
PAX6PST |
T7596 |
201-207 |
NN |
denotes |
pDBLeu |
T7595 |
198-200 |
CC |
denotes |
or |
T7594 |
190-197 |
NN |
denotes |
PAX6CTP |
T7593 |
183-189 |
NN |
denotes |
pDBLeu |
T7592 |
170-174 |
NN |
denotes |
bait |
T7591 |
175-182 |
NN |
denotes |
plasmid |
T7590 |
166-169 |
DT |
denotes |
the |
T7589 |
162-165 |
CC |
denotes |
and |
T7588 |
149-153 |
NN |
denotes |
cDNA |
T7587 |
154-161 |
NN |
denotes |
library |
T7586 |
145-148 |
DT |
denotes |
the |
T7585 |
140-144 |
IN |
denotes |
with |
T7584 |
120-124 |
VBD |
denotes |
were |
T7583 |
108-113 |
NN |
denotes |
yeast |
T7582 |
101-107 |
NN |
denotes |
MaV203 |
T7581 |
114-119 |
NNS |
denotes |
cells |
T7580 |
91-100 |
JJ |
denotes |
competent |
T7579 |
80-90 |
RB |
denotes |
chemically |
T7578 |
78-80 |
, |
denotes |
, |
T7577 |
125-139 |
VBN |
denotes |
co-transformed |
T7576 |
71-78 |
RB |
denotes |
Briefly |
T7575 |
70-216 |
sentence |
denotes |
Briefly, chemically competent MaV203 yeast cells were co-transformed with the cDNA library and the bait plasmid pDBLeu PAX6CTP or pDBLeu PAX6PST. |
T7574 |
69-70 |
. |
denotes |
. |
T7573 |
57-59 |
POS |
denotes |
's |
T7572 |
60-69 |
NNS |
denotes |
protocols |
T7571 |
49-57 |
NN |
denotes |
supplier |
T7570 |
45-48 |
DT |
denotes |
the |
T7569 |
42-44 |
IN |
denotes |
to |
T7568 |
32-41 |
VBG |
denotes |
according |
T7567 |
28-31 |
RP |
denotes |
out |
T7566 |
15-19 |
VBD |
denotes |
were |
T7565 |
20-27 |
VBN |
denotes |
carried |
T7564 |
4-14 |
NNS |
denotes |
procedures |
T7563 |
0-3 |
DT |
denotes |
All |
R4792 |
T7563 |
T7564 |
det |
All,procedures |
R4793 |
T7564 |
T7565 |
nsubjpass |
procedures,carried |
R4794 |
T7566 |
T7565 |
auxpass |
were,carried |
R4795 |
T7567 |
T7565 |
prt |
out,carried |
R4796 |
T7568 |
T7565 |
prep |
according,carried |
R4797 |
T7569 |
T7568 |
prep |
to,according |
R4798 |
T7570 |
T7571 |
det |
the,supplier |
R4799 |
T7571 |
T7572 |
poss |
supplier,protocols |
R4800 |
T7572 |
T7569 |
pobj |
protocols,to |
R4801 |
T7573 |
T7571 |
case |
's,supplier |
R4802 |
T7574 |
T7565 |
punct |
.,carried |
R4803 |
T7576 |
T7577 |
advmod |
Briefly,co-transformed |
R4804 |
T7578 |
T7577 |
punct |
", ",co-transformed |
R4805 |
T7579 |
T7580 |
advmod |
chemically,competent |
R4806 |
T7580 |
T7581 |
amod |
competent,cells |
R4807 |
T7581 |
T7577 |
nsubjpass |
cells,co-transformed |
R4808 |
T7582 |
T7581 |
compound |
MaV203,cells |
R4809 |
T7583 |
T7581 |
compound |
yeast,cells |
R4810 |
T7584 |
T7577 |
auxpass |
were,co-transformed |
R4811 |
T7585 |
T7577 |
prep |
with,co-transformed |
R4812 |
T7586 |
T7587 |
det |
the,library |
R4813 |
T7587 |
T7585 |
pobj |
library,with |
R4814 |
T7588 |
T7587 |
compound |
cDNA,library |
R4815 |
T7589 |
T7587 |
cc |
and,library |
R4816 |
T7590 |
T7591 |
det |
the,plasmid |
R4817 |
T7591 |
T7587 |
conj |
plasmid,library |
R4818 |
T7592 |
T7591 |
compound |
bait,plasmid |
R4819 |
T7593 |
T7594 |
compound |
pDBLeu,PAX6CTP |
R4820 |
T7594 |
T7591 |
appos |
PAX6CTP,plasmid |
R4821 |
T7595 |
T7594 |
cc |
or,PAX6CTP |
R4822 |
T7596 |
T7597 |
compound |
pDBLeu,PAX6PST |
R4823 |
T7597 |
T7594 |
conj |
PAX6PST,PAX6CTP |
R4824 |
T7598 |
T7577 |
punct |
.,co-transformed |
R4825 |
T7600 |
T7601 |
nsubjpass |
Transformants,plated |
R4826 |
T7602 |
T7603 |
punct |
(,107 |
R4827 |
T7603 |
T7600 |
parataxis |
107,Transformants |
R4828 |
T7604 |
T7603 |
nummod |
5,107 |
R4829 |
T7605 |
T7603 |
punct |
×,107 |
R4830 |
T7606 |
T7603 |
punct |
),107 |
R4831 |
T7607 |
T7601 |
auxpass |
were,plated |
R4832 |
T7608 |
T7601 |
prep |
on,plated |
R4833 |
T7609 |
T7608 |
pobj |
medium,on |
R4834 |
T7610 |
T7609 |
acl |
lacking,medium |
R4835 |
T7611 |
T7610 |
dobj |
histidine,lacking |
R4836 |
T7612 |
T7613 |
aux |
to,check |
R4837 |
T7613 |
T7601 |
advcl |
check,plated |
R4838 |
T7614 |
T7613 |
prep |
for,check |
R4839 |
T7615 |
T7616 |
compound |
HIS3,activation |
R4840 |
T7616 |
T7614 |
pobj |
activation,for |
R4841 |
T7617 |
T7601 |
punct |
.,plated |
R4842 |
T7619 |
T7620 |
compound |
HIS3,positives |
R4843 |
T7620 |
T7621 |
nsubjpass |
positives,assayed |
R4844 |
T7622 |
T7621 |
auxpass |
were,assayed |
R4845 |
T7623 |
T7621 |
advmod |
then,assayed |
R4846 |
T7624 |
T7621 |
prep |
for,assayed |
R4847 |
T7625 |
T7626 |
det |
all,reporters |
R4848 |
T7626 |
T7624 |
pobj |
reporters,for |
R4849 |
T7627 |
T7626 |
nummod |
3,reporters |
R4850 |
T7628 |
T7621 |
punct |
.,assayed |
R4851 |
T7630 |
T7631 |
det |
The,plasmid |
R4852 |
T7631 |
T7634 |
nsubjpass |
plasmid,isolated |
R4853 |
T7632 |
T7631 |
compound |
pPC86,plasmid |
R4854 |
T7633 |
T7631 |
compound |
prey,plasmid |
R4855 |
T7635 |
T7634 |
auxpass |
was,isolated |
R4856 |
T7636 |
T7634 |
prep |
from,isolated |
R4857 |
T7637 |
T7638 |
det |
all,positives |
R4858 |
T7638 |
T7636 |
pobj |
positives,from |
R4859 |
T7639 |
T7640 |
compound |
HIS3,LacZ |
R4860 |
T7640 |
T7638 |
compound |
LacZ,positives |
R4861 |
T7641 |
T7640 |
punct |
/,LacZ |
R4862 |
T7642 |
T7640 |
compound |
URA3,LacZ |
R4863 |
T7643 |
T7640 |
punct |
/,LacZ |
R4864 |
T7644 |
T7634 |
cc |
and,isolated |
R4865 |
T7645 |
T7646 |
det |
the,insert |
R4866 |
T7646 |
T7648 |
nsubj |
insert,sequenced |
R4867 |
T7647 |
T7646 |
compound |
cDNA,insert |
R4868 |
T7648 |
T7634 |
conj |
sequenced,isolated |
R4869 |
T7649 |
T7648 |
punct |
.,sequenced |
R4870 |
T7651 |
T7652 |
compound |
BLAST,searches |
R4871 |
T7652 |
T7653 |
nsubjpass |
searches,performed |
R4872 |
T7654 |
T7653 |
auxpass |
were,performed |
R4873 |
T7655 |
T7656 |
aux |
to,identify |
R4874 |
T7656 |
T7653 |
advcl |
identify,performed |
R4875 |
T7657 |
T7658 |
det |
the,insert |
R4876 |
T7658 |
T7656 |
dobj |
insert,identify |
R4877 |
T7659 |
T7658 |
compound |
cDNA,insert |
R4878 |
T7660 |
T7661 |
punct |
[,24 |
R4879 |
T7661 |
T7653 |
parataxis |
24,performed |
R4880 |
T7662 |
T7661 |
punct |
],24 |
R4881 |
T7663 |
T7653 |
punct |
.,performed |