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PMC:1208879 / 27618-28591 JSONTXT

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Id Subject Object Predicate Lexical cue
T7486 598-748 sentence denotes Weak URA3 activation suppresses growth on plates containing 5-fluro-orotic acid while strong URA3 activation permits growth on plates lacking uracil.
T7485 597-598 . denotes .
T7484 588-597 NN denotes histidine
T7483 580-587 VBG denotes lacking
T7482 573-579 NNS denotes plates
T7481 570-572 IN denotes on
T7480 563-569 NN denotes growth
T7479 556-562 VBZ denotes allows
T7478 545-555 NN denotes activation
T7477 540-544 NN denotes HIS3
T7476 539-598 sentence denotes HIS3 activation allows growth on plates lacking histidine.
T7475 538-539 . denotes .
T7474 530-538 NN denotes function
T7473 525-529 NN denotes GAL4
T7472 510-524 VBG denotes reconstituting
T7471 505-509 RB denotes thus
T7470 503-505 , denotes ,
T7469 502-503 -RRB- denotes )
T7468 497-502 NN denotes pPC86
T7467 496-497 -LRB- denotes (
T7466 478-488 NN denotes activation
T7465 473-477 NN denotes GAL4
T7464 489-495 NN denotes domain
T7463 469-472 DT denotes the
T7462 466-468 IN denotes to
T7461 460-465 VBN denotes fused
T7460 447-451 NN denotes prey
T7459 452-459 NN denotes protein
T7458 443-446 DT denotes the
T7457 438-442 IN denotes with
T7456 426-427 -RRB- denotes )
T7455 420-426 NN denotes pDBLeu
T7454 419-420 -LRB- denotes (
T7453 404-411 NN denotes binding
T7452 400-403 NN denotes DNA
T7451 395-399 NN denotes GAL4
T7450 412-418 NN denotes domain
T7449 391-394 DT denotes the
T7448 388-390 IN denotes to
T7447 382-387 VBN denotes fused
T7446 369-373 NN denotes bait
T7445 374-381 NN denotes protein
T7444 365-368 DT denotes the
T7443 428-437 VBZ denotes interacts
T7442 360-364 WRB denotes when
T7441 346-349 VBP denotes are
T7440 350-359 VBN denotes activated
T7439 336-345 NNS denotes Reporters
T7438 335-539 sentence denotes Reporters are activated when the bait protein fused to the GAL4 DNA binding domain (pDBLeu) interacts with the prey protein fused to the GAL4 activation domain (pPC86), thus reconstituting GAL4 function.
T7437 334-335 . denotes .
T7436 322-334 NNS denotes interactions
T7435 313-321 JJ denotes positive
T7434 304-312 VB denotes identify
T7433 301-303 TO denotes to
T7432 299-301 , denotes ,
T7431 295-299 NN denotes lacZ
T7430 291-294 CC denotes and
T7429 286-290 NN denotes URA3
T7428 284-286 , denotes ,
T7427 280-284 NN denotes HIS3
T7426 278-280 , denotes ,
T7425 264-272 NN denotes reporter
T7424 253-254 HYPH denotes -
T7423 254-263 VBN denotes activated
T7422 249-253 NN denotes GAL4
T7421 273-278 NNS denotes genes
T7420 243-248 CD denotes three
T7419 238-242 VBZ denotes uses
T7418 231-237 NN denotes system
T7417 227-230 DT denotes The
T7416 226-335 sentence denotes The system uses three GAL4-activated reporter genes, HIS3, URA3 and lacZ, to identify positive interactions.
T7415 225-226 . denotes .
T7414 208-218 NN denotes activation
T7413 203-207 NN denotes GAL4
T7412 197-202 NN denotes yeast
T7411 219-225 NN denotes domain
T7410 193-196 DT denotes the
T7409 190-192 IN denotes to
T7408 184-189 VBN denotes fused
T7407 175-183 NN denotes proteins
T7406 166-174 VBZ denotes produces
T7405 160-165 WDT denotes which
T7404 158-160 , denotes ,
T7403 146-151 NN denotes pPC86
T7402 152-158 NN denotes vector
T7401 142-145 DT denotes the
T7400 139-141 IN denotes in
T7399 123-126 VBD denotes was
T7398 127-138 VBN denotes constructed
T7397 115-122 NN denotes library
T7396 111-114 DT denotes The
T7395 110-226 sentence denotes The library was constructed in the pPC86 vector, which produces proteins fused to the yeast GAL4 activation domain.
T7394 109-110 . denotes .
T7393 92-98 NN denotes pDBLeu
T7392 84-91 NN denotes PAX6CTP
T7391 80-83 CC denotes and
T7390 72-79 NN denotes PAX6PST
T7389 99-109 NNS denotes constructs
T7388 68-71 DT denotes the
T7387 63-67 IN denotes with
T7386 50-53 VBD denotes was
T7385 48-49 -RRB- denotes )
T7384 38-48 NNP denotes Invitrogen
T7383 36-38 , denotes ,
T7382 28-36 NNP denotes ProQuest
T7381 27-28 -LRB- denotes (
T7380 54-62 VBN denotes screened
T7379 14-18 NN denotes cDNA
T7378 8-13 NN denotes brain
T7377 2-7 NN denotes mouse
T7376 19-26 NN denotes library
T7375 0-1 DT denotes A
T7561 972-973 . denotes .
T7560 971-972 -RRB- denotes )
T7559 970-971 NN denotes E
T7558 969-970 -LRB- denotes (
T7557 958-968 NN denotes interactor
T7556 951-957 JJ denotes strong
T7555 948-950 IN denotes to
T7554 946-947 -RRB- denotes )
T7553 945-946 NN denotes A
T7552 944-945 -LRB- denotes (
T7551 929-943 NN denotes non-interactor
T7550 924-928 IN denotes from
T7549 918-923 VBP denotes range
T7548 912-917 WDT denotes which
T7547 910-912 , denotes ,
T7546 909-910 NN denotes E
T7545 908-909 SYM denotes -
T7544 907-908 NN denotes A
T7543 893-898 NN denotes yeast
T7542 885-892 NN denotes control
T7541 876-884 NNP denotes ProQuest
T7540 871-875 CD denotes five
T7539 899-906 NNS denotes strains
T7538 867-870 DT denotes the
T7537 862-866 IN denotes with
T7536 853-861 NN denotes parallel
T7535 850-852 IN denotes in
T7534 846-849 RP denotes out
T7533 833-837 VBD denotes were
T7532 838-845 VBN denotes carried
T7531 826-832 NNS denotes assays
T7530 822-825 DT denotes All
T7529 821-973 sentence denotes All assays were carried out in parallel with the five ProQuest control yeast strains A-E, which range from non-interactor (A) to strong interactor (E).
T7528 820-821 . denotes .
T7527 800-801 HYPH denotes -
T7526 801-814 NN denotes galactosidase
T7525 796-800 NN denotes beta
T7524 815-820 NN denotes assay
T7523 794-795 DT denotes a
T7522 791-793 IN denotes in
T7521 786-790 JJ denotes blue
T7520 778-780 TO denotes to
T7519 781-785 VB denotes turn
T7518 773-774 HYPH denotes -
T7517 774-777 NN denotes gal
T7516 772-773 NN denotes X
T7515 765-771 VBZ denotes causes
T7514 754-764 NN denotes activation
T7513 749-753 NN denotes LacZ
T7512 748-821 sentence denotes LacZ activation causes X-gal to turn blue in a beta-galactosidase assay.
T7511 747-748 . denotes .
T7510 741-747 NN denotes uracil
T7509 733-740 VBG denotes lacking
T7508 726-732 NNS denotes plates
T7507 723-725 IN denotes on
T7506 716-722 NN denotes growth
T7505 692-696 NN denotes URA3
T7504 697-707 NN denotes activation
T7503 685-691 JJ denotes strong
T7502 708-715 VBZ denotes permits
T7501 679-684 IN denotes while
T7500 674-678 NN denotes acid
T7499 666-667 HYPH denotes -
T7498 661-666 JJ denotes fluro
T7497 660-661 HYPH denotes -
T7496 667-673 JJ denotes orotic
T7495 659-660 CD denotes 5
T7494 648-658 VBG denotes containing
T7493 641-647 NNS denotes plates
T7492 638-640 IN denotes on
T7491 631-637 NN denotes growth
T7490 620-630 VBZ denotes suppresses
T7489 604-608 NN denotes URA3
T7488 609-619 NN denotes activation
T7487 599-603 JJ denotes Weak
R4620 T7375 T7376 det A,library
R4621 T7376 T7380 nsubjpass library,screened
R4622 T7377 T7378 compound mouse,brain
R4623 T7378 T7376 compound brain,library
R4624 T7379 T7376 compound cDNA,library
R4625 T7381 T7382 punct (,ProQuest
R4626 T7382 T7376 parataxis ProQuest,library
R4627 T7383 T7382 punct ", ",ProQuest
R4628 T7384 T7382 npadvmod Invitrogen,ProQuest
R4629 T7385 T7382 punct ),ProQuest
R4630 T7386 T7380 auxpass was,screened
R4631 T7387 T7380 prep with,screened
R4632 T7388 T7389 det the,constructs
R4633 T7389 T7387 pobj constructs,with
R4634 T7390 T7389 nmod PAX6PST,constructs
R4635 T7391 T7390 cc and,PAX6PST
R4636 T7392 T7390 conj PAX6CTP,PAX6PST
R4637 T7393 T7389 compound pDBLeu,constructs
R4638 T7394 T7380 punct .,screened
R4639 T7396 T7397 det The,library
R4640 T7397 T7398 nsubjpass library,constructed
R4641 T7399 T7398 auxpass was,constructed
R4642 T7400 T7398 prep in,constructed
R4643 T7401 T7402 det the,vector
R4644 T7402 T7400 pobj vector,in
R4645 T7403 T7402 compound pPC86,vector
R4646 T7404 T7402 punct ", ",vector
R4647 T7405 T7406 dep which,produces
R4648 T7406 T7402 relcl produces,vector
R4649 T7407 T7406 dobj proteins,produces
R4650 T7408 T7407 acl fused,proteins
R4651 T7409 T7408 prep to,fused
R4652 T7410 T7411 det the,domain
R4653 T7411 T7409 pobj domain,to
R4654 T7412 T7411 compound yeast,domain
R4655 T7413 T7411 compound GAL4,domain
R4656 T7414 T7411 compound activation,domain
R4657 T7415 T7398 punct .,constructed
R4658 T7417 T7418 det The,system
R4659 T7418 T7419 nsubj system,uses
R4660 T7420 T7421 nummod three,genes
R4661 T7421 T7419 dobj genes,uses
R4662 T7422 T7423 npadvmod GAL4,activated
R4663 T7423 T7421 amod activated,genes
R4664 T7424 T7423 punct -,activated
R4665 T7425 T7421 compound reporter,genes
R4666 T7426 T7421 punct ", ",genes
R4667 T7427 T7421 appos HIS3,genes
R4668 T7428 T7427 punct ", ",HIS3
R4669 T7429 T7427 conj URA3,HIS3
R4670 T7430 T7429 cc and,URA3
R4671 T7431 T7429 conj lacZ,URA3
R4672 T7432 T7419 punct ", ",uses
R4673 T7433 T7434 aux to,identify
R4674 T7434 T7419 advcl identify,uses
R4675 T7435 T7436 amod positive,interactions
R4676 T7436 T7434 dobj interactions,identify
R4677 T7437 T7419 punct .,uses
R4678 T7439 T7440 nsubjpass Reporters,activated
R4679 T7441 T7440 auxpass are,activated
R4680 T7442 T7443 advmod when,interacts
R4681 T7443 T7440 advcl interacts,activated
R4682 T7444 T7445 det the,protein
R4683 T7445 T7443 nsubj protein,interacts
R4684 T7446 T7445 compound bait,protein
R4685 T7447 T7445 acl fused,protein
R4686 T7448 T7447 prep to,fused
R4687 T7449 T7450 det the,domain
R4688 T7450 T7448 pobj domain,to
R4689 T7451 T7450 compound GAL4,domain
R4690 T7452 T7450 compound DNA,domain
R4691 T7453 T7450 compound binding,domain
R4692 T7454 T7450 punct (,domain
R4693 T7455 T7450 appos pDBLeu,domain
R4694 T7456 T7450 punct ),domain
R4695 T7457 T7443 prep with,interacts
R4696 T7458 T7459 det the,protein
R4697 T7459 T7457 pobj protein,with
R4698 T7460 T7459 compound prey,protein
R4699 T7461 T7459 acl fused,protein
R4700 T7462 T7461 prep to,fused
R4701 T7463 T7464 det the,domain
R4702 T7464 T7462 pobj domain,to
R4703 T7465 T7464 compound GAL4,domain
R4704 T7466 T7464 compound activation,domain
R4705 T7467 T7464 punct (,domain
R4706 T7468 T7464 appos pPC86,domain
R4707 T7469 T7464 punct ),domain
R4708 T7470 T7440 punct ", ",activated
R4709 T7471 T7472 advmod thus,reconstituting
R4710 T7472 T7440 advcl reconstituting,activated
R4711 T7473 T7474 compound GAL4,function
R4712 T7474 T7472 dobj function,reconstituting
R4713 T7475 T7440 punct .,activated
R4714 T7477 T7478 compound HIS3,activation
R4715 T7478 T7479 nsubj activation,allows
R4716 T7480 T7479 dobj growth,allows
R4717 T7481 T7479 prep on,allows
R4718 T7482 T7481 pobj plates,on
R4719 T7483 T7482 acl lacking,plates
R4720 T7484 T7483 dobj histidine,lacking
R4721 T7485 T7479 punct .,allows
R4722 T7487 T7488 amod Weak,activation
R4723 T7488 T7490 nsubj activation,suppresses
R4724 T7489 T7488 compound URA3,activation
R4725 T7491 T7490 dobj growth,suppresses
R4726 T7492 T7490 prep on,suppresses
R4727 T7493 T7492 pobj plates,on
R4728 T7494 T7493 acl containing,plates
R4729 T7495 T7496 advmod 5,orotic
R4730 T7496 T7500 amod orotic,acid
R4731 T7497 T7496 punct -,orotic
R4732 T7498 T7496 amod fluro,orotic
R4733 T7499 T7496 punct -,orotic
R4734 T7500 T7494 dobj acid,containing
R4735 T7501 T7502 mark while,permits
R4736 T7502 T7490 advcl permits,suppresses
R4737 T7503 T7504 amod strong,activation
R4738 T7504 T7502 nsubj activation,permits
R4739 T7505 T7504 compound URA3,activation
R4740 T7506 T7502 dobj growth,permits
R4741 T7507 T7502 prep on,permits
R4742 T7508 T7507 pobj plates,on
R4743 T7509 T7508 acl lacking,plates
R4744 T7510 T7509 dobj uracil,lacking
R4745 T7511 T7490 punct .,suppresses
R4746 T7513 T7514 compound LacZ,activation
R4747 T7514 T7515 nsubj activation,causes
R4748 T7516 T7517 compound X,gal
R4749 T7517 T7519 nsubj gal,turn
R4750 T7518 T7517 punct -,gal
R4751 T7519 T7515 ccomp turn,causes
R4752 T7520 T7519 aux to,turn
R4753 T7521 T7519 oprd blue,turn
R4754 T7522 T7521 prep in,blue
R4755 T7523 T7524 det a,assay
R4756 T7524 T7522 pobj assay,in
R4757 T7525 T7526 compound beta,galactosidase
R4758 T7526 T7524 compound galactosidase,assay
R4759 T7527 T7526 punct -,galactosidase
R4760 T7528 T7515 punct .,causes
R4761 T7530 T7531 det All,assays
R4762 T7531 T7532 nsubjpass assays,carried
R4763 T7533 T7532 auxpass were,carried
R4764 T7534 T7532 prt out,carried
R4765 T7535 T7532 prep in,carried
R4766 T7536 T7535 pobj parallel,in
R4767 T7537 T7536 prep with,parallel
R4768 T7538 T7539 det the,strains
R4769 T7539 T7537 pobj strains,with
R4770 T7540 T7539 nummod five,strains
R4771 T7541 T7539 compound ProQuest,strains
R4772 T7542 T7539 compound control,strains
R4773 T7543 T7539 compound yeast,strains
R4774 T7544 T7539 appos A,strains
R4775 T7545 T7546 punct -,E
R4776 T7546 T7544 prep E,A
R4777 T7547 T7539 punct ", ",strains
R4778 T7548 T7549 dep which,range
R4779 T7549 T7539 relcl range,strains
R4780 T7550 T7549 prep from,range
R4781 T7551 T7550 pobj non-interactor,from
R4782 T7552 T7553 punct (,A
R4783 T7553 T7551 parataxis A,non-interactor
R4784 T7554 T7553 punct ),A
R4785 T7555 T7550 prep to,from
R4786 T7556 T7557 amod strong,interactor
R4787 T7557 T7555 pobj interactor,to
R4788 T7558 T7559 punct (,E
R4789 T7559 T7557 parataxis E,interactor
R4790 T7560 T7559 punct ),E
R4791 T7561 T7532 punct .,carried

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7349 893-898 NCBITaxon_EXT:yeast denotes yeast
T7348 796-814 GO_EXT:0004565 denotes beta-galactosidase
T7347 772-777 CHEBI:75055 denotes X-gal
T7346 749-753 PR_EXT:000033987 denotes LacZ
T7345 741-747 CHEBI_EXT:uracil_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes uracil
T7344 716-722 GO_EXT:biological_growth_entity_or_process denotes growth
T7343 692-696 PR_EXT:P03962 denotes URA3
T7342 659-678 CHEBI:74498 denotes 5-fluro-orotic acid
T7341 631-637 GO_EXT:biological_growth_entity_or_process denotes growth
T7340 604-608 PR_EXT:P03962 denotes URA3
T7339 588-597 CHEBI_SO_EXT:histidine denotes histidine
T7338 563-569 GO_EXT:biological_growth_entity_or_process denotes growth
T7337 540-544 PR_EXT:P06633 denotes HIS3
T7336 525-529 PR_EXT:P04386 denotes GAL4
T7335 489-495 SO_EXT:0000417 denotes domain
T7334 473-477 PR_EXT:P04386 denotes GAL4
T7333 460-465 SO_EXT:sequence_fusion_process denotes fused
T7332 452-459 CHEBI_PR_EXT:protein denotes protein
T7331 412-418 SO_EXT:0000417 denotes domain
T7330 404-411 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T7329 400-403 CHEBI_SO_EXT:DNA denotes DNA
T7328 395-399 PR_EXT:P04386 denotes GAL4
T7327 382-387 SO_EXT:sequence_fusion_process denotes fused
T7326 374-381 CHEBI_PR_EXT:protein denotes protein
T7325 295-299 PR_EXT:000033987 denotes lacZ
T7324 286-290 PR_EXT:P03962 denotes URA3
T7323 280-284 PR_EXT:P06633 denotes HIS3
T7322 273-278 SO_EXT:0000704 denotes genes
T7321 249-253 PR_EXT:P04386 denotes GAL4
T7320 219-225 SO_EXT:0000417 denotes domain
T7319 203-207 PR_EXT:P04386 denotes GAL4
T7318 197-202 NCBITaxon_EXT:yeast denotes yeast
T7317 184-189 SO_EXT:sequence_fusion_process denotes fused
T7316 175-183 CHEBI_PR_EXT:protein denotes proteins
T7315 152-158 SO_EXT:0000440 denotes vector
T7314 99-109 SO_EXT:engineered_biological_sequence denotes constructs
T7313 14-18 SO_EXT:cDNA denotes cDNA
T7312 8-13 UBERON:0000955 denotes brain
T7311 2-7 NCBITaxon:10088 denotes mouse

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7299 772-777 CHEBI:75055 denotes X-gal
T7298 749-753 PR:000033987 denotes LacZ
T7297 692-696 PR:P03962 denotes URA3
T7296 659-678 CHEBI:74498 denotes 5-fluro-orotic acid
T7295 604-608 PR:P03962 denotes URA3
T7294 540-544 PR:P06633 denotes HIS3
T7293 525-529 PR:P04386 denotes GAL4
T7292 489-495 SO:0000417 denotes domain
T7291 473-477 PR:P04386 denotes GAL4
T7290 412-418 SO:0000417 denotes domain
T7289 395-399 PR:P04386 denotes GAL4
T7288 295-299 PR:000033987 denotes lacZ
T7287 286-290 PR:P03962 denotes URA3
T7286 280-284 PR:P06633 denotes HIS3
T7285 273-278 SO:0000704 denotes genes
T7284 249-253 PR:P04386 denotes GAL4
T7283 219-225 SO:0000417 denotes domain
T7282 203-207 PR:P04386 denotes GAL4
T7281 152-158 SO:0000440 denotes vector
T7280 8-13 UBERON:0000955 denotes brain
T7279 2-7 NCBITaxon:10088 denotes mouse