Id |
Subject |
Object |
Predicate |
Lexical cue |
T7663 |
1535-1536 |
. |
denotes |
. |
T7662 |
1534-1535 |
-RRB- |
denotes |
] |
T7661 |
1532-1534 |
CD |
denotes |
24 |
T7660 |
1531-1532 |
-LRB- |
denotes |
[ |
T7659 |
1519-1523 |
NN |
denotes |
cDNA |
T7486 |
631-781 |
sentence |
denotes |
Weak URA3 activation suppresses growth on plates containing 5-fluro-orotic acid while strong URA3 activation permits growth on plates lacking uracil. |
T7485 |
630-631 |
. |
denotes |
. |
T7484 |
621-630 |
NN |
denotes |
histidine |
T7483 |
613-620 |
VBG |
denotes |
lacking |
T7482 |
606-612 |
NNS |
denotes |
plates |
T7481 |
603-605 |
IN |
denotes |
on |
T7480 |
596-602 |
NN |
denotes |
growth |
T7479 |
589-595 |
VBZ |
denotes |
allows |
T7478 |
578-588 |
NN |
denotes |
activation |
T7477 |
573-577 |
NN |
denotes |
HIS3 |
T7476 |
572-631 |
sentence |
denotes |
HIS3 activation allows growth on plates lacking histidine. |
T7475 |
571-572 |
. |
denotes |
. |
T7474 |
563-571 |
NN |
denotes |
function |
T7473 |
558-562 |
NN |
denotes |
GAL4 |
T7472 |
543-557 |
VBG |
denotes |
reconstituting |
T7471 |
538-542 |
RB |
denotes |
thus |
T7470 |
536-538 |
, |
denotes |
, |
T7469 |
535-536 |
-RRB- |
denotes |
) |
T7468 |
530-535 |
NN |
denotes |
pPC86 |
T7467 |
529-530 |
-LRB- |
denotes |
( |
T7466 |
511-521 |
NN |
denotes |
activation |
T7465 |
506-510 |
NN |
denotes |
GAL4 |
T7464 |
522-528 |
NN |
denotes |
domain |
T7463 |
502-505 |
DT |
denotes |
the |
T7462 |
499-501 |
IN |
denotes |
to |
T7461 |
493-498 |
VBN |
denotes |
fused |
T7460 |
480-484 |
NN |
denotes |
prey |
T7459 |
485-492 |
NN |
denotes |
protein |
T7458 |
476-479 |
DT |
denotes |
the |
T7457 |
471-475 |
IN |
denotes |
with |
T7456 |
459-460 |
-RRB- |
denotes |
) |
T7455 |
453-459 |
NN |
denotes |
pDBLeu |
T7454 |
452-453 |
-LRB- |
denotes |
( |
T7453 |
437-444 |
NN |
denotes |
binding |
T7452 |
433-436 |
NN |
denotes |
DNA |
T7451 |
428-432 |
NN |
denotes |
GAL4 |
T7450 |
445-451 |
NN |
denotes |
domain |
T7449 |
424-427 |
DT |
denotes |
the |
T7448 |
421-423 |
IN |
denotes |
to |
T7447 |
415-420 |
VBN |
denotes |
fused |
T7446 |
402-406 |
NN |
denotes |
bait |
T7445 |
407-414 |
NN |
denotes |
protein |
T7444 |
398-401 |
DT |
denotes |
the |
T7443 |
461-470 |
VBZ |
denotes |
interacts |
T7442 |
393-397 |
WRB |
denotes |
when |
T7441 |
379-382 |
VBP |
denotes |
are |
T7440 |
383-392 |
VBN |
denotes |
activated |
T7439 |
369-378 |
NNS |
denotes |
Reporters |
T7438 |
368-572 |
sentence |
denotes |
Reporters are activated when the bait protein fused to the GAL4 DNA binding domain (pDBLeu) interacts with the prey protein fused to the GAL4 activation domain (pPC86), thus reconstituting GAL4 function. |
T7437 |
367-368 |
. |
denotes |
. |
T7436 |
355-367 |
NNS |
denotes |
interactions |
T7435 |
346-354 |
JJ |
denotes |
positive |
T7434 |
337-345 |
VB |
denotes |
identify |
T7433 |
334-336 |
TO |
denotes |
to |
T7432 |
332-334 |
, |
denotes |
, |
T7431 |
328-332 |
NN |
denotes |
lacZ |
T7430 |
324-327 |
CC |
denotes |
and |
T7429 |
319-323 |
NN |
denotes |
URA3 |
T7428 |
317-319 |
, |
denotes |
, |
T7427 |
313-317 |
NN |
denotes |
HIS3 |
T7426 |
311-313 |
, |
denotes |
, |
T7425 |
297-305 |
NN |
denotes |
reporter |
T7424 |
286-287 |
HYPH |
denotes |
- |
T7423 |
287-296 |
VBN |
denotes |
activated |
T7422 |
282-286 |
NN |
denotes |
GAL4 |
T7421 |
306-311 |
NNS |
denotes |
genes |
T7420 |
276-281 |
CD |
denotes |
three |
T7419 |
271-275 |
VBZ |
denotes |
uses |
T7418 |
264-270 |
NN |
denotes |
system |
T7417 |
260-263 |
DT |
denotes |
The |
T7416 |
259-368 |
sentence |
denotes |
The system uses three GAL4-activated reporter genes, HIS3, URA3 and lacZ, to identify positive interactions. |
T7415 |
258-259 |
. |
denotes |
. |
T7414 |
241-251 |
NN |
denotes |
activation |
T7413 |
236-240 |
NN |
denotes |
GAL4 |
T7412 |
230-235 |
NN |
denotes |
yeast |
T7411 |
252-258 |
NN |
denotes |
domain |
T7410 |
226-229 |
DT |
denotes |
the |
T7409 |
223-225 |
IN |
denotes |
to |
T7408 |
217-222 |
VBN |
denotes |
fused |
T7407 |
208-216 |
NN |
denotes |
proteins |
T7406 |
199-207 |
VBZ |
denotes |
produces |
T7405 |
193-198 |
WDT |
denotes |
which |
T7404 |
191-193 |
, |
denotes |
, |
T7403 |
179-184 |
NN |
denotes |
pPC86 |
T7402 |
185-191 |
NN |
denotes |
vector |
T7401 |
175-178 |
DT |
denotes |
the |
T7400 |
172-174 |
IN |
denotes |
in |
T7399 |
156-159 |
VBD |
denotes |
was |
T7398 |
160-171 |
VBN |
denotes |
constructed |
T7397 |
148-155 |
NN |
denotes |
library |
T7396 |
144-147 |
DT |
denotes |
The |
T7395 |
143-259 |
sentence |
denotes |
The library was constructed in the pPC86 vector, which produces proteins fused to the yeast GAL4 activation domain. |
T7394 |
142-143 |
. |
denotes |
. |
T7393 |
125-131 |
NN |
denotes |
pDBLeu |
T7392 |
117-124 |
NN |
denotes |
PAX6CTP |
T7391 |
113-116 |
CC |
denotes |
and |
T7390 |
105-112 |
NN |
denotes |
PAX6PST |
T7389 |
132-142 |
NNS |
denotes |
constructs |
T7388 |
101-104 |
DT |
denotes |
the |
T7387 |
96-100 |
IN |
denotes |
with |
T7386 |
83-86 |
VBD |
denotes |
was |
T7385 |
81-82 |
-RRB- |
denotes |
) |
T7384 |
71-81 |
NNP |
denotes |
Invitrogen |
T7383 |
69-71 |
, |
denotes |
, |
T7382 |
61-69 |
NNP |
denotes |
ProQuest |
T7381 |
60-61 |
-LRB- |
denotes |
( |
T7380 |
87-95 |
VBN |
denotes |
screened |
T7379 |
47-51 |
NN |
denotes |
cDNA |
T7378 |
41-46 |
NN |
denotes |
brain |
T7377 |
35-40 |
NN |
denotes |
mouse |
T7376 |
52-59 |
NN |
denotes |
library |
T7375 |
33-34 |
DT |
denotes |
A |
T7374 |
32-143 |
sentence |
denotes |
A mouse brain cDNA library (ProQuest, Invitrogen) was screened with the PAX6PST and PAX6CTP pDBLeu constructs. |
T7373 |
17-24 |
NN |
denotes |
library |
T7372 |
9-10 |
HYPH |
denotes |
- |
T7371 |
10-16 |
NN |
denotes |
hybrid |
T7370 |
6-9 |
CD |
denotes |
two |
T7369 |
25-32 |
NNS |
denotes |
screens |
T7368 |
0-5 |
NN |
denotes |
Yeast |
T7658 |
1524-1530 |
NN |
denotes |
insert |
T7657 |
1515-1518 |
DT |
denotes |
the |
T7656 |
1506-1514 |
VB |
denotes |
identify |
T7655 |
1503-1505 |
TO |
denotes |
to |
T7654 |
1488-1492 |
VBD |
denotes |
were |
T7653 |
1493-1502 |
VBN |
denotes |
performed |
T7652 |
1479-1487 |
NNS |
denotes |
searches |
T7651 |
1473-1478 |
NNP |
denotes |
BLAST |
T7650 |
1472-1536 |
sentence |
denotes |
BLAST searches were performed to identify the cDNA insert [24]. |
T7649 |
1471-1472 |
. |
denotes |
. |
T7648 |
1462-1471 |
VBN |
denotes |
sequenced |
T7647 |
1450-1454 |
NN |
denotes |
cDNA |
T7646 |
1455-1461 |
NN |
denotes |
insert |
T7645 |
1446-1449 |
DT |
denotes |
the |
T7644 |
1442-1445 |
CC |
denotes |
and |
T7643 |
1426-1427 |
HYPH |
denotes |
/ |
T7642 |
1422-1426 |
NN |
denotes |
URA3 |
T7641 |
1421-1422 |
HYPH |
denotes |
/ |
T7640 |
1427-1431 |
NN |
denotes |
LacZ |
T7639 |
1417-1421 |
NN |
denotes |
HIS3 |
T7638 |
1432-1441 |
NNS |
denotes |
positives |
T7637 |
1413-1416 |
DT |
denotes |
all |
T7636 |
1408-1412 |
IN |
denotes |
from |
T7635 |
1395-1398 |
VBD |
denotes |
was |
T7634 |
1399-1407 |
VBN |
denotes |
isolated |
T7633 |
1382-1386 |
NN |
denotes |
prey |
T7632 |
1376-1381 |
NN |
denotes |
pPC86 |
T7631 |
1387-1394 |
NN |
denotes |
plasmid |
T7630 |
1372-1375 |
DT |
denotes |
The |
T7629 |
1371-1472 |
sentence |
denotes |
The pPC86 prey plasmid was isolated from all HIS3/URA3/LacZ positives and the cDNA insert sequenced. |
T7628 |
1370-1371 |
. |
denotes |
. |
T7627 |
1359-1360 |
CD |
denotes |
3 |
T7626 |
1361-1370 |
NNS |
denotes |
reporters |
T7625 |
1355-1358 |
DT |
denotes |
all |
T7624 |
1351-1354 |
IN |
denotes |
for |
T7623 |
1338-1342 |
RB |
denotes |
then |
T7622 |
1333-1337 |
VBD |
denotes |
were |
T7621 |
1343-1350 |
VBN |
denotes |
assayed |
T7620 |
1323-1332 |
NNS |
denotes |
positives |
T7619 |
1318-1322 |
NN |
denotes |
HIS3 |
T7618 |
1317-1371 |
sentence |
denotes |
HIS3 positives were then assayed for all 3 reporters. |
T7617 |
1316-1317 |
. |
denotes |
. |
T7616 |
1306-1316 |
NN |
denotes |
activation |
T7615 |
1301-1305 |
NN |
denotes |
HIS3 |
T7614 |
1297-1300 |
IN |
denotes |
for |
T7613 |
1291-1296 |
VB |
denotes |
check |
T7612 |
1288-1290 |
TO |
denotes |
to |
T7611 |
1278-1287 |
NN |
denotes |
histidine |
T7610 |
1270-1277 |
VBG |
denotes |
lacking |
T7609 |
1263-1269 |
NN |
denotes |
medium |
T7608 |
1260-1262 |
IN |
denotes |
on |
T7607 |
1248-1252 |
VBD |
denotes |
were |
T7606 |
1246-1247 |
-RRB- |
denotes |
) |
T7605 |
1241-1242 |
SYM |
denotes |
× |
T7604 |
1239-1240 |
CD |
denotes |
5 |
T7603 |
1243-1246 |
CD |
denotes |
107 |
T7602 |
1238-1239 |
-LRB- |
denotes |
( |
T7601 |
1253-1259 |
VBN |
denotes |
plated |
T7600 |
1224-1237 |
NNS |
denotes |
Transformants |
T7599 |
1223-1317 |
sentence |
denotes |
Transformants (5 × 107) were plated on medium lacking histidine to check for HIS3 activation. |
T7598 |
1222-1223 |
. |
denotes |
. |
T7597 |
1215-1222 |
NN |
denotes |
PAX6PST |
T7596 |
1208-1214 |
NN |
denotes |
pDBLeu |
T7595 |
1205-1207 |
CC |
denotes |
or |
T7594 |
1197-1204 |
NN |
denotes |
PAX6CTP |
T7593 |
1190-1196 |
NN |
denotes |
pDBLeu |
T7592 |
1177-1181 |
NN |
denotes |
bait |
T7591 |
1182-1189 |
NN |
denotes |
plasmid |
T7590 |
1173-1176 |
DT |
denotes |
the |
T7589 |
1169-1172 |
CC |
denotes |
and |
T7588 |
1156-1160 |
NN |
denotes |
cDNA |
T7587 |
1161-1168 |
NN |
denotes |
library |
T7586 |
1152-1155 |
DT |
denotes |
the |
T7585 |
1147-1151 |
IN |
denotes |
with |
T7584 |
1127-1131 |
VBD |
denotes |
were |
T7583 |
1115-1120 |
NN |
denotes |
yeast |
T7582 |
1108-1114 |
NN |
denotes |
MaV203 |
T7581 |
1121-1126 |
NNS |
denotes |
cells |
T7580 |
1098-1107 |
JJ |
denotes |
competent |
T7579 |
1087-1097 |
RB |
denotes |
chemically |
T7578 |
1085-1087 |
, |
denotes |
, |
T7577 |
1132-1146 |
VBN |
denotes |
co-transformed |
T7576 |
1078-1085 |
RB |
denotes |
Briefly |
T7575 |
1077-1223 |
sentence |
denotes |
Briefly, chemically competent MaV203 yeast cells were co-transformed with the cDNA library and the bait plasmid pDBLeu PAX6CTP or pDBLeu PAX6PST. |
T7574 |
1076-1077 |
. |
denotes |
. |
T7573 |
1064-1066 |
POS |
denotes |
's |
T7572 |
1067-1076 |
NNS |
denotes |
protocols |
T7571 |
1056-1064 |
NN |
denotes |
supplier |
T7570 |
1052-1055 |
DT |
denotes |
the |
T7569 |
1049-1051 |
IN |
denotes |
to |
T7568 |
1039-1048 |
VBG |
denotes |
according |
T7567 |
1035-1038 |
RP |
denotes |
out |
T7566 |
1022-1026 |
VBD |
denotes |
were |
T7565 |
1027-1034 |
VBN |
denotes |
carried |
T7564 |
1011-1021 |
NNS |
denotes |
procedures |
T7563 |
1007-1010 |
DT |
denotes |
All |
T7562 |
1006-1077 |
sentence |
denotes |
All procedures were carried out according to the supplier's protocols. |
T7561 |
1005-1006 |
. |
denotes |
. |
T7560 |
1004-1005 |
-RRB- |
denotes |
) |
T7559 |
1003-1004 |
NN |
denotes |
E |
T7558 |
1002-1003 |
-LRB- |
denotes |
( |
T7557 |
991-1001 |
NN |
denotes |
interactor |
T7556 |
984-990 |
JJ |
denotes |
strong |
T7555 |
981-983 |
IN |
denotes |
to |
T7554 |
979-980 |
-RRB- |
denotes |
) |
T7553 |
978-979 |
NN |
denotes |
A |
T7552 |
977-978 |
-LRB- |
denotes |
( |
T7551 |
962-976 |
NN |
denotes |
non-interactor |
T7550 |
957-961 |
IN |
denotes |
from |
T7549 |
951-956 |
VBP |
denotes |
range |
T7548 |
945-950 |
WDT |
denotes |
which |
T7547 |
943-945 |
, |
denotes |
, |
T7546 |
942-943 |
NN |
denotes |
E |
T7545 |
941-942 |
SYM |
denotes |
- |
T7544 |
940-941 |
NN |
denotes |
A |
T7543 |
926-931 |
NN |
denotes |
yeast |
T7542 |
918-925 |
NN |
denotes |
control |
T7541 |
909-917 |
NNP |
denotes |
ProQuest |
T7540 |
904-908 |
CD |
denotes |
five |
T7539 |
932-939 |
NNS |
denotes |
strains |
T7538 |
900-903 |
DT |
denotes |
the |
T7537 |
895-899 |
IN |
denotes |
with |
T7536 |
886-894 |
NN |
denotes |
parallel |
T7535 |
883-885 |
IN |
denotes |
in |
T7534 |
879-882 |
RP |
denotes |
out |
T7533 |
866-870 |
VBD |
denotes |
were |
T7532 |
871-878 |
VBN |
denotes |
carried |
T7531 |
859-865 |
NNS |
denotes |
assays |
T7530 |
855-858 |
DT |
denotes |
All |
T7529 |
854-1006 |
sentence |
denotes |
All assays were carried out in parallel with the five ProQuest control yeast strains A-E, which range from non-interactor (A) to strong interactor (E). |
T7528 |
853-854 |
. |
denotes |
. |
T7527 |
833-834 |
HYPH |
denotes |
- |
T7526 |
834-847 |
NN |
denotes |
galactosidase |
T7525 |
829-833 |
NN |
denotes |
beta |
T7524 |
848-853 |
NN |
denotes |
assay |
T7523 |
827-828 |
DT |
denotes |
a |
T7522 |
824-826 |
IN |
denotes |
in |
T7521 |
819-823 |
JJ |
denotes |
blue |
T7520 |
811-813 |
TO |
denotes |
to |
T7519 |
814-818 |
VB |
denotes |
turn |
T7518 |
806-807 |
HYPH |
denotes |
- |
T7517 |
807-810 |
NN |
denotes |
gal |
T7516 |
805-806 |
NN |
denotes |
X |
T7515 |
798-804 |
VBZ |
denotes |
causes |
T7514 |
787-797 |
NN |
denotes |
activation |
T7513 |
782-786 |
NN |
denotes |
LacZ |
T7512 |
781-854 |
sentence |
denotes |
LacZ activation causes X-gal to turn blue in a beta-galactosidase assay. |
T7511 |
780-781 |
. |
denotes |
. |
T7510 |
774-780 |
NN |
denotes |
uracil |
T7509 |
766-773 |
VBG |
denotes |
lacking |
T7508 |
759-765 |
NNS |
denotes |
plates |
T7507 |
756-758 |
IN |
denotes |
on |
T7506 |
749-755 |
NN |
denotes |
growth |
T7505 |
725-729 |
NN |
denotes |
URA3 |
T7504 |
730-740 |
NN |
denotes |
activation |
T7503 |
718-724 |
JJ |
denotes |
strong |
T7502 |
741-748 |
VBZ |
denotes |
permits |
T7501 |
712-717 |
IN |
denotes |
while |
T7500 |
707-711 |
NN |
denotes |
acid |
T7499 |
699-700 |
HYPH |
denotes |
- |
T7498 |
694-699 |
JJ |
denotes |
fluro |
T7497 |
693-694 |
HYPH |
denotes |
- |
T7496 |
700-706 |
JJ |
denotes |
orotic |
T7495 |
692-693 |
CD |
denotes |
5 |
T7494 |
681-691 |
VBG |
denotes |
containing |
T7493 |
674-680 |
NNS |
denotes |
plates |
T7492 |
671-673 |
IN |
denotes |
on |
T7491 |
664-670 |
NN |
denotes |
growth |
T7490 |
653-663 |
VBZ |
denotes |
suppresses |
T7489 |
637-641 |
NN |
denotes |
URA3 |
T7488 |
642-652 |
NN |
denotes |
activation |
T7487 |
632-636 |
JJ |
denotes |
Weak |
R4615 |
T7368 |
T7369 |
nmod |
Yeast,screens |
R4616 |
T7370 |
T7371 |
nummod |
two,hybrid |
R4617 |
T7371 |
T7369 |
compound |
hybrid,screens |
R4618 |
T7372 |
T7371 |
punct |
-,hybrid |
R4619 |
T7373 |
T7369 |
compound |
library,screens |
R4620 |
T7375 |
T7376 |
det |
A,library |
R4621 |
T7376 |
T7380 |
nsubjpass |
library,screened |
R4622 |
T7377 |
T7378 |
compound |
mouse,brain |
R4623 |
T7378 |
T7376 |
compound |
brain,library |
R4624 |
T7379 |
T7376 |
compound |
cDNA,library |
R4625 |
T7381 |
T7382 |
punct |
(,ProQuest |
R4626 |
T7382 |
T7376 |
parataxis |
ProQuest,library |
R4627 |
T7383 |
T7382 |
punct |
", ",ProQuest |
R4628 |
T7384 |
T7382 |
npadvmod |
Invitrogen,ProQuest |
R4629 |
T7385 |
T7382 |
punct |
),ProQuest |
R4630 |
T7386 |
T7380 |
auxpass |
was,screened |
R4631 |
T7387 |
T7380 |
prep |
with,screened |
R4632 |
T7388 |
T7389 |
det |
the,constructs |
R4633 |
T7389 |
T7387 |
pobj |
constructs,with |
R4634 |
T7390 |
T7389 |
nmod |
PAX6PST,constructs |
R4635 |
T7391 |
T7390 |
cc |
and,PAX6PST |
R4636 |
T7392 |
T7390 |
conj |
PAX6CTP,PAX6PST |
R4637 |
T7393 |
T7389 |
compound |
pDBLeu,constructs |
R4638 |
T7394 |
T7380 |
punct |
.,screened |
R4639 |
T7396 |
T7397 |
det |
The,library |
R4640 |
T7397 |
T7398 |
nsubjpass |
library,constructed |
R4641 |
T7399 |
T7398 |
auxpass |
was,constructed |
R4642 |
T7400 |
T7398 |
prep |
in,constructed |
R4643 |
T7401 |
T7402 |
det |
the,vector |
R4644 |
T7402 |
T7400 |
pobj |
vector,in |
R4645 |
T7403 |
T7402 |
compound |
pPC86,vector |
R4646 |
T7404 |
T7402 |
punct |
", ",vector |
R4647 |
T7405 |
T7406 |
dep |
which,produces |
R4648 |
T7406 |
T7402 |
relcl |
produces,vector |
R4649 |
T7407 |
T7406 |
dobj |
proteins,produces |
R4650 |
T7408 |
T7407 |
acl |
fused,proteins |
R4651 |
T7409 |
T7408 |
prep |
to,fused |
R4652 |
T7410 |
T7411 |
det |
the,domain |
R4653 |
T7411 |
T7409 |
pobj |
domain,to |
R4654 |
T7412 |
T7411 |
compound |
yeast,domain |
R4655 |
T7413 |
T7411 |
compound |
GAL4,domain |
R4656 |
T7414 |
T7411 |
compound |
activation,domain |
R4657 |
T7415 |
T7398 |
punct |
.,constructed |
R4658 |
T7417 |
T7418 |
det |
The,system |
R4659 |
T7418 |
T7419 |
nsubj |
system,uses |
R4660 |
T7420 |
T7421 |
nummod |
three,genes |
R4661 |
T7421 |
T7419 |
dobj |
genes,uses |
R4662 |
T7422 |
T7423 |
npadvmod |
GAL4,activated |
R4663 |
T7423 |
T7421 |
amod |
activated,genes |
R4664 |
T7424 |
T7423 |
punct |
-,activated |
R4665 |
T7425 |
T7421 |
compound |
reporter,genes |
R4666 |
T7426 |
T7421 |
punct |
", ",genes |
R4667 |
T7427 |
T7421 |
appos |
HIS3,genes |
R4668 |
T7428 |
T7427 |
punct |
", ",HIS3 |
R4669 |
T7429 |
T7427 |
conj |
URA3,HIS3 |
R4670 |
T7430 |
T7429 |
cc |
and,URA3 |
R4671 |
T7431 |
T7429 |
conj |
lacZ,URA3 |
R4672 |
T7432 |
T7419 |
punct |
", ",uses |
R4673 |
T7433 |
T7434 |
aux |
to,identify |
R4674 |
T7434 |
T7419 |
advcl |
identify,uses |
R4675 |
T7435 |
T7436 |
amod |
positive,interactions |
R4676 |
T7436 |
T7434 |
dobj |
interactions,identify |
R4677 |
T7437 |
T7419 |
punct |
.,uses |
R4678 |
T7439 |
T7440 |
nsubjpass |
Reporters,activated |
R4679 |
T7441 |
T7440 |
auxpass |
are,activated |
R4680 |
T7442 |
T7443 |
advmod |
when,interacts |
R4681 |
T7443 |
T7440 |
advcl |
interacts,activated |
R4682 |
T7444 |
T7445 |
det |
the,protein |
R4683 |
T7445 |
T7443 |
nsubj |
protein,interacts |
R4684 |
T7446 |
T7445 |
compound |
bait,protein |
R4685 |
T7447 |
T7445 |
acl |
fused,protein |
R4686 |
T7448 |
T7447 |
prep |
to,fused |
R4687 |
T7449 |
T7450 |
det |
the,domain |
R4688 |
T7450 |
T7448 |
pobj |
domain,to |
R4689 |
T7451 |
T7450 |
compound |
GAL4,domain |
R4690 |
T7452 |
T7450 |
compound |
DNA,domain |
R4691 |
T7453 |
T7450 |
compound |
binding,domain |
R4692 |
T7454 |
T7450 |
punct |
(,domain |
R4693 |
T7455 |
T7450 |
appos |
pDBLeu,domain |
R4694 |
T7456 |
T7450 |
punct |
),domain |
R4695 |
T7457 |
T7443 |
prep |
with,interacts |
R4696 |
T7458 |
T7459 |
det |
the,protein |
R4697 |
T7459 |
T7457 |
pobj |
protein,with |
R4698 |
T7460 |
T7459 |
compound |
prey,protein |
R4699 |
T7461 |
T7459 |
acl |
fused,protein |
R4700 |
T7462 |
T7461 |
prep |
to,fused |
R4701 |
T7463 |
T7464 |
det |
the,domain |
R4702 |
T7464 |
T7462 |
pobj |
domain,to |
R4703 |
T7465 |
T7464 |
compound |
GAL4,domain |
R4704 |
T7466 |
T7464 |
compound |
activation,domain |
R4705 |
T7467 |
T7464 |
punct |
(,domain |
R4706 |
T7468 |
T7464 |
appos |
pPC86,domain |
R4707 |
T7469 |
T7464 |
punct |
),domain |
R4708 |
T7470 |
T7440 |
punct |
", ",activated |
R4709 |
T7471 |
T7472 |
advmod |
thus,reconstituting |
R4710 |
T7472 |
T7440 |
advcl |
reconstituting,activated |
R4711 |
T7473 |
T7474 |
compound |
GAL4,function |
R4712 |
T7474 |
T7472 |
dobj |
function,reconstituting |
R4713 |
T7475 |
T7440 |
punct |
.,activated |
R4714 |
T7477 |
T7478 |
compound |
HIS3,activation |
R4715 |
T7478 |
T7479 |
nsubj |
activation,allows |
R4716 |
T7480 |
T7479 |
dobj |
growth,allows |
R4717 |
T7481 |
T7479 |
prep |
on,allows |
R4718 |
T7482 |
T7481 |
pobj |
plates,on |
R4719 |
T7483 |
T7482 |
acl |
lacking,plates |
R4720 |
T7484 |
T7483 |
dobj |
histidine,lacking |
R4721 |
T7485 |
T7479 |
punct |
.,allows |
R4722 |
T7487 |
T7488 |
amod |
Weak,activation |
R4723 |
T7488 |
T7490 |
nsubj |
activation,suppresses |
R4724 |
T7489 |
T7488 |
compound |
URA3,activation |
R4725 |
T7491 |
T7490 |
dobj |
growth,suppresses |
R4726 |
T7492 |
T7490 |
prep |
on,suppresses |
R4727 |
T7493 |
T7492 |
pobj |
plates,on |
R4728 |
T7494 |
T7493 |
acl |
containing,plates |
R4729 |
T7495 |
T7496 |
advmod |
5,orotic |
R4730 |
T7496 |
T7500 |
amod |
orotic,acid |
R4731 |
T7497 |
T7496 |
punct |
-,orotic |
R4732 |
T7498 |
T7496 |
amod |
fluro,orotic |
R4733 |
T7499 |
T7496 |
punct |
-,orotic |
R4734 |
T7500 |
T7494 |
dobj |
acid,containing |
R4735 |
T7501 |
T7502 |
mark |
while,permits |
R4736 |
T7502 |
T7490 |
advcl |
permits,suppresses |
R4737 |
T7503 |
T7504 |
amod |
strong,activation |
R4738 |
T7504 |
T7502 |
nsubj |
activation,permits |
R4739 |
T7505 |
T7504 |
compound |
URA3,activation |
R4740 |
T7506 |
T7502 |
dobj |
growth,permits |
R4741 |
T7507 |
T7502 |
prep |
on,permits |
R4742 |
T7508 |
T7507 |
pobj |
plates,on |
R4743 |
T7509 |
T7508 |
acl |
lacking,plates |
R4744 |
T7510 |
T7509 |
dobj |
uracil,lacking |
R4745 |
T7511 |
T7490 |
punct |
.,suppresses |
R4746 |
T7513 |
T7514 |
compound |
LacZ,activation |
R4747 |
T7514 |
T7515 |
nsubj |
activation,causes |
R4748 |
T7516 |
T7517 |
compound |
X,gal |
R4749 |
T7517 |
T7519 |
nsubj |
gal,turn |
R4750 |
T7518 |
T7517 |
punct |
-,gal |
R4751 |
T7519 |
T7515 |
ccomp |
turn,causes |
R4752 |
T7520 |
T7519 |
aux |
to,turn |
R4753 |
T7521 |
T7519 |
oprd |
blue,turn |
R4754 |
T7522 |
T7521 |
prep |
in,blue |
R4755 |
T7523 |
T7524 |
det |
a,assay |
R4756 |
T7524 |
T7522 |
pobj |
assay,in |
R4757 |
T7525 |
T7526 |
compound |
beta,galactosidase |
R4758 |
T7526 |
T7524 |
compound |
galactosidase,assay |
R4759 |
T7527 |
T7526 |
punct |
-,galactosidase |
R4760 |
T7528 |
T7515 |
punct |
.,causes |
R4761 |
T7530 |
T7531 |
det |
All,assays |
R4762 |
T7531 |
T7532 |
nsubjpass |
assays,carried |
R4763 |
T7533 |
T7532 |
auxpass |
were,carried |
R4764 |
T7534 |
T7532 |
prt |
out,carried |
R4765 |
T7535 |
T7532 |
prep |
in,carried |
R4766 |
T7536 |
T7535 |
pobj |
parallel,in |
R4767 |
T7537 |
T7536 |
prep |
with,parallel |
R4768 |
T7538 |
T7539 |
det |
the,strains |
R4769 |
T7539 |
T7537 |
pobj |
strains,with |
R4770 |
T7540 |
T7539 |
nummod |
five,strains |
R4771 |
T7541 |
T7539 |
compound |
ProQuest,strains |
R4772 |
T7542 |
T7539 |
compound |
control,strains |
R4773 |
T7543 |
T7539 |
compound |
yeast,strains |
R4774 |
T7544 |
T7539 |
appos |
A,strains |
R4775 |
T7545 |
T7546 |
punct |
-,E |
R4776 |
T7546 |
T7544 |
prep |
E,A |
R4777 |
T7547 |
T7539 |
punct |
", ",strains |
R4778 |
T7548 |
T7549 |
dep |
which,range |
R4779 |
T7549 |
T7539 |
relcl |
range,strains |
R4780 |
T7550 |
T7549 |
prep |
from,range |
R4781 |
T7551 |
T7550 |
pobj |
non-interactor,from |
R4782 |
T7552 |
T7553 |
punct |
(,A |
R4783 |
T7553 |
T7551 |
parataxis |
A,non-interactor |
R4784 |
T7554 |
T7553 |
punct |
),A |
R4785 |
T7555 |
T7550 |
prep |
to,from |
R4786 |
T7556 |
T7557 |
amod |
strong,interactor |
R4787 |
T7557 |
T7555 |
pobj |
interactor,to |
R4788 |
T7558 |
T7559 |
punct |
(,E |
R4789 |
T7559 |
T7557 |
parataxis |
E,interactor |
R4790 |
T7560 |
T7559 |
punct |
),E |
R4791 |
T7561 |
T7532 |
punct |
.,carried |
R4792 |
T7563 |
T7564 |
det |
All,procedures |
R4793 |
T7564 |
T7565 |
nsubjpass |
procedures,carried |
R4794 |
T7566 |
T7565 |
auxpass |
were,carried |
R4795 |
T7567 |
T7565 |
prt |
out,carried |
R4796 |
T7568 |
T7565 |
prep |
according,carried |
R4797 |
T7569 |
T7568 |
prep |
to,according |
R4798 |
T7570 |
T7571 |
det |
the,supplier |
R4799 |
T7571 |
T7572 |
poss |
supplier,protocols |
R4800 |
T7572 |
T7569 |
pobj |
protocols,to |
R4801 |
T7573 |
T7571 |
case |
's,supplier |
R4802 |
T7574 |
T7565 |
punct |
.,carried |
R4803 |
T7576 |
T7577 |
advmod |
Briefly,co-transformed |
R4804 |
T7578 |
T7577 |
punct |
", ",co-transformed |
R4805 |
T7579 |
T7580 |
advmod |
chemically,competent |
R4806 |
T7580 |
T7581 |
amod |
competent,cells |
R4807 |
T7581 |
T7577 |
nsubjpass |
cells,co-transformed |
R4808 |
T7582 |
T7581 |
compound |
MaV203,cells |
R4809 |
T7583 |
T7581 |
compound |
yeast,cells |
R4810 |
T7584 |
T7577 |
auxpass |
were,co-transformed |
R4811 |
T7585 |
T7577 |
prep |
with,co-transformed |
R4812 |
T7586 |
T7587 |
det |
the,library |
R4813 |
T7587 |
T7585 |
pobj |
library,with |
R4814 |
T7588 |
T7587 |
compound |
cDNA,library |
R4815 |
T7589 |
T7587 |
cc |
and,library |
R4816 |
T7590 |
T7591 |
det |
the,plasmid |
R4817 |
T7591 |
T7587 |
conj |
plasmid,library |
R4818 |
T7592 |
T7591 |
compound |
bait,plasmid |
R4819 |
T7593 |
T7594 |
compound |
pDBLeu,PAX6CTP |
R4820 |
T7594 |
T7591 |
appos |
PAX6CTP,plasmid |
R4821 |
T7595 |
T7594 |
cc |
or,PAX6CTP |
R4822 |
T7596 |
T7597 |
compound |
pDBLeu,PAX6PST |
R4823 |
T7597 |
T7594 |
conj |
PAX6PST,PAX6CTP |
R4824 |
T7598 |
T7577 |
punct |
.,co-transformed |
R4825 |
T7600 |
T7601 |
nsubjpass |
Transformants,plated |
R4826 |
T7602 |
T7603 |
punct |
(,107 |
R4827 |
T7603 |
T7600 |
parataxis |
107,Transformants |
R4828 |
T7604 |
T7603 |
nummod |
5,107 |
R4829 |
T7605 |
T7603 |
punct |
×,107 |
R4830 |
T7606 |
T7603 |
punct |
),107 |
R4831 |
T7607 |
T7601 |
auxpass |
were,plated |
R4832 |
T7608 |
T7601 |
prep |
on,plated |
R4833 |
T7609 |
T7608 |
pobj |
medium,on |
R4834 |
T7610 |
T7609 |
acl |
lacking,medium |
R4835 |
T7611 |
T7610 |
dobj |
histidine,lacking |
R4836 |
T7612 |
T7613 |
aux |
to,check |
R4837 |
T7613 |
T7601 |
advcl |
check,plated |
R4838 |
T7614 |
T7613 |
prep |
for,check |
R4839 |
T7615 |
T7616 |
compound |
HIS3,activation |
R4840 |
T7616 |
T7614 |
pobj |
activation,for |
R4841 |
T7617 |
T7601 |
punct |
.,plated |
R4842 |
T7619 |
T7620 |
compound |
HIS3,positives |
R4843 |
T7620 |
T7621 |
nsubjpass |
positives,assayed |
R4844 |
T7622 |
T7621 |
auxpass |
were,assayed |
R4845 |
T7623 |
T7621 |
advmod |
then,assayed |
R4846 |
T7624 |
T7621 |
prep |
for,assayed |
R4847 |
T7625 |
T7626 |
det |
all,reporters |
R4848 |
T7626 |
T7624 |
pobj |
reporters,for |
R4849 |
T7627 |
T7626 |
nummod |
3,reporters |
R4850 |
T7628 |
T7621 |
punct |
.,assayed |
R4851 |
T7630 |
T7631 |
det |
The,plasmid |
R4852 |
T7631 |
T7634 |
nsubjpass |
plasmid,isolated |
R4853 |
T7632 |
T7631 |
compound |
pPC86,plasmid |
R4854 |
T7633 |
T7631 |
compound |
prey,plasmid |
R4855 |
T7635 |
T7634 |
auxpass |
was,isolated |
R4856 |
T7636 |
T7634 |
prep |
from,isolated |
R4857 |
T7637 |
T7638 |
det |
all,positives |
R4858 |
T7638 |
T7636 |
pobj |
positives,from |
R4859 |
T7639 |
T7640 |
compound |
HIS3,LacZ |
R4860 |
T7640 |
T7638 |
compound |
LacZ,positives |
R4861 |
T7641 |
T7640 |
punct |
/,LacZ |
R4862 |
T7642 |
T7640 |
compound |
URA3,LacZ |
R4863 |
T7643 |
T7640 |
punct |
/,LacZ |
R4864 |
T7644 |
T7634 |
cc |
and,isolated |
R4865 |
T7645 |
T7646 |
det |
the,insert |
R4866 |
T7646 |
T7648 |
nsubj |
insert,sequenced |
R4867 |
T7647 |
T7646 |
compound |
cDNA,insert |
R4868 |
T7648 |
T7634 |
conj |
sequenced,isolated |
R4869 |
T7649 |
T7648 |
punct |
.,sequenced |
R4870 |
T7651 |
T7652 |
compound |
BLAST,searches |
R4871 |
T7652 |
T7653 |
nsubjpass |
searches,performed |
R4872 |
T7654 |
T7653 |
auxpass |
were,performed |
R4873 |
T7655 |
T7656 |
aux |
to,identify |
R4874 |
T7656 |
T7653 |
advcl |
identify,performed |
R4875 |
T7657 |
T7658 |
det |
the,insert |
R4876 |
T7658 |
T7656 |
dobj |
insert,identify |
R4877 |
T7659 |
T7658 |
compound |
cDNA,insert |
R4878 |
T7660 |
T7661 |
punct |
[,24 |
R4879 |
T7661 |
T7653 |
parataxis |
24,performed |
R4880 |
T7662 |
T7661 |
punct |
],24 |
R4881 |
T7663 |
T7653 |
punct |
.,performed |