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Id Subject Object Predicate Lexical cue
T7663 1535-1536 . denotes .
T7662 1534-1535 -RRB- denotes ]
T7661 1532-1534 CD denotes 24
T7660 1531-1532 -LRB- denotes [
T7659 1519-1523 NN denotes cDNA
T7486 631-781 sentence denotes Weak URA3 activation suppresses growth on plates containing 5-fluro-orotic acid while strong URA3 activation permits growth on plates lacking uracil.
T7485 630-631 . denotes .
T7484 621-630 NN denotes histidine
T7483 613-620 VBG denotes lacking
T7482 606-612 NNS denotes plates
T7481 603-605 IN denotes on
T7480 596-602 NN denotes growth
T7479 589-595 VBZ denotes allows
T7478 578-588 NN denotes activation
T7477 573-577 NN denotes HIS3
T7476 572-631 sentence denotes HIS3 activation allows growth on plates lacking histidine.
T7475 571-572 . denotes .
T7474 563-571 NN denotes function
T7473 558-562 NN denotes GAL4
T7472 543-557 VBG denotes reconstituting
T7471 538-542 RB denotes thus
T7470 536-538 , denotes ,
T7469 535-536 -RRB- denotes )
T7468 530-535 NN denotes pPC86
T7467 529-530 -LRB- denotes (
T7466 511-521 NN denotes activation
T7465 506-510 NN denotes GAL4
T7464 522-528 NN denotes domain
T7463 502-505 DT denotes the
T7462 499-501 IN denotes to
T7461 493-498 VBN denotes fused
T7460 480-484 NN denotes prey
T7459 485-492 NN denotes protein
T7458 476-479 DT denotes the
T7457 471-475 IN denotes with
T7456 459-460 -RRB- denotes )
T7455 453-459 NN denotes pDBLeu
T7454 452-453 -LRB- denotes (
T7453 437-444 NN denotes binding
T7452 433-436 NN denotes DNA
T7451 428-432 NN denotes GAL4
T7450 445-451 NN denotes domain
T7449 424-427 DT denotes the
T7448 421-423 IN denotes to
T7447 415-420 VBN denotes fused
T7446 402-406 NN denotes bait
T7445 407-414 NN denotes protein
T7444 398-401 DT denotes the
T7443 461-470 VBZ denotes interacts
T7442 393-397 WRB denotes when
T7441 379-382 VBP denotes are
T7440 383-392 VBN denotes activated
T7439 369-378 NNS denotes Reporters
T7438 368-572 sentence denotes Reporters are activated when the bait protein fused to the GAL4 DNA binding domain (pDBLeu) interacts with the prey protein fused to the GAL4 activation domain (pPC86), thus reconstituting GAL4 function.
T7437 367-368 . denotes .
T7436 355-367 NNS denotes interactions
T7435 346-354 JJ denotes positive
T7434 337-345 VB denotes identify
T7433 334-336 TO denotes to
T7432 332-334 , denotes ,
T7431 328-332 NN denotes lacZ
T7430 324-327 CC denotes and
T7429 319-323 NN denotes URA3
T7428 317-319 , denotes ,
T7427 313-317 NN denotes HIS3
T7426 311-313 , denotes ,
T7425 297-305 NN denotes reporter
T7424 286-287 HYPH denotes -
T7423 287-296 VBN denotes activated
T7422 282-286 NN denotes GAL4
T7421 306-311 NNS denotes genes
T7420 276-281 CD denotes three
T7419 271-275 VBZ denotes uses
T7418 264-270 NN denotes system
T7417 260-263 DT denotes The
T7416 259-368 sentence denotes The system uses three GAL4-activated reporter genes, HIS3, URA3 and lacZ, to identify positive interactions.
T7415 258-259 . denotes .
T7414 241-251 NN denotes activation
T7413 236-240 NN denotes GAL4
T7412 230-235 NN denotes yeast
T7411 252-258 NN denotes domain
T7410 226-229 DT denotes the
T7409 223-225 IN denotes to
T7408 217-222 VBN denotes fused
T7407 208-216 NN denotes proteins
T7406 199-207 VBZ denotes produces
T7405 193-198 WDT denotes which
T7404 191-193 , denotes ,
T7403 179-184 NN denotes pPC86
T7402 185-191 NN denotes vector
T7401 175-178 DT denotes the
T7400 172-174 IN denotes in
T7399 156-159 VBD denotes was
T7398 160-171 VBN denotes constructed
T7397 148-155 NN denotes library
T7396 144-147 DT denotes The
T7395 143-259 sentence denotes The library was constructed in the pPC86 vector, which produces proteins fused to the yeast GAL4 activation domain.
T7394 142-143 . denotes .
T7393 125-131 NN denotes pDBLeu
T7392 117-124 NN denotes PAX6CTP
T7391 113-116 CC denotes and
T7390 105-112 NN denotes PAX6PST
T7389 132-142 NNS denotes constructs
T7388 101-104 DT denotes the
T7387 96-100 IN denotes with
T7386 83-86 VBD denotes was
T7385 81-82 -RRB- denotes )
T7384 71-81 NNP denotes Invitrogen
T7383 69-71 , denotes ,
T7382 61-69 NNP denotes ProQuest
T7381 60-61 -LRB- denotes (
T7380 87-95 VBN denotes screened
T7379 47-51 NN denotes cDNA
T7378 41-46 NN denotes brain
T7377 35-40 NN denotes mouse
T7376 52-59 NN denotes library
T7375 33-34 DT denotes A
T7374 32-143 sentence denotes A mouse brain cDNA library (ProQuest, Invitrogen) was screened with the PAX6PST and PAX6CTP pDBLeu constructs.
T7373 17-24 NN denotes library
T7372 9-10 HYPH denotes -
T7371 10-16 NN denotes hybrid
T7370 6-9 CD denotes two
T7369 25-32 NNS denotes screens
T7368 0-5 NN denotes Yeast
T7658 1524-1530 NN denotes insert
T7657 1515-1518 DT denotes the
T7656 1506-1514 VB denotes identify
T7655 1503-1505 TO denotes to
T7654 1488-1492 VBD denotes were
T7653 1493-1502 VBN denotes performed
T7652 1479-1487 NNS denotes searches
T7651 1473-1478 NNP denotes BLAST
T7650 1472-1536 sentence denotes BLAST searches were performed to identify the cDNA insert [24].
T7649 1471-1472 . denotes .
T7648 1462-1471 VBN denotes sequenced
T7647 1450-1454 NN denotes cDNA
T7646 1455-1461 NN denotes insert
T7645 1446-1449 DT denotes the
T7644 1442-1445 CC denotes and
T7643 1426-1427 HYPH denotes /
T7642 1422-1426 NN denotes URA3
T7641 1421-1422 HYPH denotes /
T7640 1427-1431 NN denotes LacZ
T7639 1417-1421 NN denotes HIS3
T7638 1432-1441 NNS denotes positives
T7637 1413-1416 DT denotes all
T7636 1408-1412 IN denotes from
T7635 1395-1398 VBD denotes was
T7634 1399-1407 VBN denotes isolated
T7633 1382-1386 NN denotes prey
T7632 1376-1381 NN denotes pPC86
T7631 1387-1394 NN denotes plasmid
T7630 1372-1375 DT denotes The
T7629 1371-1472 sentence denotes The pPC86 prey plasmid was isolated from all HIS3/URA3/LacZ positives and the cDNA insert sequenced.
T7628 1370-1371 . denotes .
T7627 1359-1360 CD denotes 3
T7626 1361-1370 NNS denotes reporters
T7625 1355-1358 DT denotes all
T7624 1351-1354 IN denotes for
T7623 1338-1342 RB denotes then
T7622 1333-1337 VBD denotes were
T7621 1343-1350 VBN denotes assayed
T7620 1323-1332 NNS denotes positives
T7619 1318-1322 NN denotes HIS3
T7618 1317-1371 sentence denotes HIS3 positives were then assayed for all 3 reporters.
T7617 1316-1317 . denotes .
T7616 1306-1316 NN denotes activation
T7615 1301-1305 NN denotes HIS3
T7614 1297-1300 IN denotes for
T7613 1291-1296 VB denotes check
T7612 1288-1290 TO denotes to
T7611 1278-1287 NN denotes histidine
T7610 1270-1277 VBG denotes lacking
T7609 1263-1269 NN denotes medium
T7608 1260-1262 IN denotes on
T7607 1248-1252 VBD denotes were
T7606 1246-1247 -RRB- denotes )
T7605 1241-1242 SYM denotes ×
T7604 1239-1240 CD denotes 5
T7603 1243-1246 CD denotes 107
T7602 1238-1239 -LRB- denotes (
T7601 1253-1259 VBN denotes plated
T7600 1224-1237 NNS denotes Transformants
T7599 1223-1317 sentence denotes Transformants (5 × 107) were plated on medium lacking histidine to check for HIS3 activation.
T7598 1222-1223 . denotes .
T7597 1215-1222 NN denotes PAX6PST
T7596 1208-1214 NN denotes pDBLeu
T7595 1205-1207 CC denotes or
T7594 1197-1204 NN denotes PAX6CTP
T7593 1190-1196 NN denotes pDBLeu
T7592 1177-1181 NN denotes bait
T7591 1182-1189 NN denotes plasmid
T7590 1173-1176 DT denotes the
T7589 1169-1172 CC denotes and
T7588 1156-1160 NN denotes cDNA
T7587 1161-1168 NN denotes library
T7586 1152-1155 DT denotes the
T7585 1147-1151 IN denotes with
T7584 1127-1131 VBD denotes were
T7583 1115-1120 NN denotes yeast
T7582 1108-1114 NN denotes MaV203
T7581 1121-1126 NNS denotes cells
T7580 1098-1107 JJ denotes competent
T7579 1087-1097 RB denotes chemically
T7578 1085-1087 , denotes ,
T7577 1132-1146 VBN denotes co-transformed
T7576 1078-1085 RB denotes Briefly
T7575 1077-1223 sentence denotes Briefly, chemically competent MaV203 yeast cells were co-transformed with the cDNA library and the bait plasmid pDBLeu PAX6CTP or pDBLeu PAX6PST.
T7574 1076-1077 . denotes .
T7573 1064-1066 POS denotes 's
T7572 1067-1076 NNS denotes protocols
T7571 1056-1064 NN denotes supplier
T7570 1052-1055 DT denotes the
T7569 1049-1051 IN denotes to
T7568 1039-1048 VBG denotes according
T7567 1035-1038 RP denotes out
T7566 1022-1026 VBD denotes were
T7565 1027-1034 VBN denotes carried
T7564 1011-1021 NNS denotes procedures
T7563 1007-1010 DT denotes All
T7562 1006-1077 sentence denotes All procedures were carried out according to the supplier's protocols.
T7561 1005-1006 . denotes .
T7560 1004-1005 -RRB- denotes )
T7559 1003-1004 NN denotes E
T7558 1002-1003 -LRB- denotes (
T7557 991-1001 NN denotes interactor
T7556 984-990 JJ denotes strong
T7555 981-983 IN denotes to
T7554 979-980 -RRB- denotes )
T7553 978-979 NN denotes A
T7552 977-978 -LRB- denotes (
T7551 962-976 NN denotes non-interactor
T7550 957-961 IN denotes from
T7549 951-956 VBP denotes range
T7548 945-950 WDT denotes which
T7547 943-945 , denotes ,
T7546 942-943 NN denotes E
T7545 941-942 SYM denotes -
T7544 940-941 NN denotes A
T7543 926-931 NN denotes yeast
T7542 918-925 NN denotes control
T7541 909-917 NNP denotes ProQuest
T7540 904-908 CD denotes five
T7539 932-939 NNS denotes strains
T7538 900-903 DT denotes the
T7537 895-899 IN denotes with
T7536 886-894 NN denotes parallel
T7535 883-885 IN denotes in
T7534 879-882 RP denotes out
T7533 866-870 VBD denotes were
T7532 871-878 VBN denotes carried
T7531 859-865 NNS denotes assays
T7530 855-858 DT denotes All
T7529 854-1006 sentence denotes All assays were carried out in parallel with the five ProQuest control yeast strains A-E, which range from non-interactor (A) to strong interactor (E).
T7528 853-854 . denotes .
T7527 833-834 HYPH denotes -
T7526 834-847 NN denotes galactosidase
T7525 829-833 NN denotes beta
T7524 848-853 NN denotes assay
T7523 827-828 DT denotes a
T7522 824-826 IN denotes in
T7521 819-823 JJ denotes blue
T7520 811-813 TO denotes to
T7519 814-818 VB denotes turn
T7518 806-807 HYPH denotes -
T7517 807-810 NN denotes gal
T7516 805-806 NN denotes X
T7515 798-804 VBZ denotes causes
T7514 787-797 NN denotes activation
T7513 782-786 NN denotes LacZ
T7512 781-854 sentence denotes LacZ activation causes X-gal to turn blue in a beta-galactosidase assay.
T7511 780-781 . denotes .
T7510 774-780 NN denotes uracil
T7509 766-773 VBG denotes lacking
T7508 759-765 NNS denotes plates
T7507 756-758 IN denotes on
T7506 749-755 NN denotes growth
T7505 725-729 NN denotes URA3
T7504 730-740 NN denotes activation
T7503 718-724 JJ denotes strong
T7502 741-748 VBZ denotes permits
T7501 712-717 IN denotes while
T7500 707-711 NN denotes acid
T7499 699-700 HYPH denotes -
T7498 694-699 JJ denotes fluro
T7497 693-694 HYPH denotes -
T7496 700-706 JJ denotes orotic
T7495 692-693 CD denotes 5
T7494 681-691 VBG denotes containing
T7493 674-680 NNS denotes plates
T7492 671-673 IN denotes on
T7491 664-670 NN denotes growth
T7490 653-663 VBZ denotes suppresses
T7489 637-641 NN denotes URA3
T7488 642-652 NN denotes activation
T7487 632-636 JJ denotes Weak
R4615 T7368 T7369 nmod Yeast,screens
R4616 T7370 T7371 nummod two,hybrid
R4617 T7371 T7369 compound hybrid,screens
R4618 T7372 T7371 punct -,hybrid
R4619 T7373 T7369 compound library,screens
R4620 T7375 T7376 det A,library
R4621 T7376 T7380 nsubjpass library,screened
R4622 T7377 T7378 compound mouse,brain
R4623 T7378 T7376 compound brain,library
R4624 T7379 T7376 compound cDNA,library
R4625 T7381 T7382 punct (,ProQuest
R4626 T7382 T7376 parataxis ProQuest,library
R4627 T7383 T7382 punct ", ",ProQuest
R4628 T7384 T7382 npadvmod Invitrogen,ProQuest
R4629 T7385 T7382 punct ),ProQuest
R4630 T7386 T7380 auxpass was,screened
R4631 T7387 T7380 prep with,screened
R4632 T7388 T7389 det the,constructs
R4633 T7389 T7387 pobj constructs,with
R4634 T7390 T7389 nmod PAX6PST,constructs
R4635 T7391 T7390 cc and,PAX6PST
R4636 T7392 T7390 conj PAX6CTP,PAX6PST
R4637 T7393 T7389 compound pDBLeu,constructs
R4638 T7394 T7380 punct .,screened
R4639 T7396 T7397 det The,library
R4640 T7397 T7398 nsubjpass library,constructed
R4641 T7399 T7398 auxpass was,constructed
R4642 T7400 T7398 prep in,constructed
R4643 T7401 T7402 det the,vector
R4644 T7402 T7400 pobj vector,in
R4645 T7403 T7402 compound pPC86,vector
R4646 T7404 T7402 punct ", ",vector
R4647 T7405 T7406 dep which,produces
R4648 T7406 T7402 relcl produces,vector
R4649 T7407 T7406 dobj proteins,produces
R4650 T7408 T7407 acl fused,proteins
R4651 T7409 T7408 prep to,fused
R4652 T7410 T7411 det the,domain
R4653 T7411 T7409 pobj domain,to
R4654 T7412 T7411 compound yeast,domain
R4655 T7413 T7411 compound GAL4,domain
R4656 T7414 T7411 compound activation,domain
R4657 T7415 T7398 punct .,constructed
R4658 T7417 T7418 det The,system
R4659 T7418 T7419 nsubj system,uses
R4660 T7420 T7421 nummod three,genes
R4661 T7421 T7419 dobj genes,uses
R4662 T7422 T7423 npadvmod GAL4,activated
R4663 T7423 T7421 amod activated,genes
R4664 T7424 T7423 punct -,activated
R4665 T7425 T7421 compound reporter,genes
R4666 T7426 T7421 punct ", ",genes
R4667 T7427 T7421 appos HIS3,genes
R4668 T7428 T7427 punct ", ",HIS3
R4669 T7429 T7427 conj URA3,HIS3
R4670 T7430 T7429 cc and,URA3
R4671 T7431 T7429 conj lacZ,URA3
R4672 T7432 T7419 punct ", ",uses
R4673 T7433 T7434 aux to,identify
R4674 T7434 T7419 advcl identify,uses
R4675 T7435 T7436 amod positive,interactions
R4676 T7436 T7434 dobj interactions,identify
R4677 T7437 T7419 punct .,uses
R4678 T7439 T7440 nsubjpass Reporters,activated
R4679 T7441 T7440 auxpass are,activated
R4680 T7442 T7443 advmod when,interacts
R4681 T7443 T7440 advcl interacts,activated
R4682 T7444 T7445 det the,protein
R4683 T7445 T7443 nsubj protein,interacts
R4684 T7446 T7445 compound bait,protein
R4685 T7447 T7445 acl fused,protein
R4686 T7448 T7447 prep to,fused
R4687 T7449 T7450 det the,domain
R4688 T7450 T7448 pobj domain,to
R4689 T7451 T7450 compound GAL4,domain
R4690 T7452 T7450 compound DNA,domain
R4691 T7453 T7450 compound binding,domain
R4692 T7454 T7450 punct (,domain
R4693 T7455 T7450 appos pDBLeu,domain
R4694 T7456 T7450 punct ),domain
R4695 T7457 T7443 prep with,interacts
R4696 T7458 T7459 det the,protein
R4697 T7459 T7457 pobj protein,with
R4698 T7460 T7459 compound prey,protein
R4699 T7461 T7459 acl fused,protein
R4700 T7462 T7461 prep to,fused
R4701 T7463 T7464 det the,domain
R4702 T7464 T7462 pobj domain,to
R4703 T7465 T7464 compound GAL4,domain
R4704 T7466 T7464 compound activation,domain
R4705 T7467 T7464 punct (,domain
R4706 T7468 T7464 appos pPC86,domain
R4707 T7469 T7464 punct ),domain
R4708 T7470 T7440 punct ", ",activated
R4709 T7471 T7472 advmod thus,reconstituting
R4710 T7472 T7440 advcl reconstituting,activated
R4711 T7473 T7474 compound GAL4,function
R4712 T7474 T7472 dobj function,reconstituting
R4713 T7475 T7440 punct .,activated
R4714 T7477 T7478 compound HIS3,activation
R4715 T7478 T7479 nsubj activation,allows
R4716 T7480 T7479 dobj growth,allows
R4717 T7481 T7479 prep on,allows
R4718 T7482 T7481 pobj plates,on
R4719 T7483 T7482 acl lacking,plates
R4720 T7484 T7483 dobj histidine,lacking
R4721 T7485 T7479 punct .,allows
R4722 T7487 T7488 amod Weak,activation
R4723 T7488 T7490 nsubj activation,suppresses
R4724 T7489 T7488 compound URA3,activation
R4725 T7491 T7490 dobj growth,suppresses
R4726 T7492 T7490 prep on,suppresses
R4727 T7493 T7492 pobj plates,on
R4728 T7494 T7493 acl containing,plates
R4729 T7495 T7496 advmod 5,orotic
R4730 T7496 T7500 amod orotic,acid
R4731 T7497 T7496 punct -,orotic
R4732 T7498 T7496 amod fluro,orotic
R4733 T7499 T7496 punct -,orotic
R4734 T7500 T7494 dobj acid,containing
R4735 T7501 T7502 mark while,permits
R4736 T7502 T7490 advcl permits,suppresses
R4737 T7503 T7504 amod strong,activation
R4738 T7504 T7502 nsubj activation,permits
R4739 T7505 T7504 compound URA3,activation
R4740 T7506 T7502 dobj growth,permits
R4741 T7507 T7502 prep on,permits
R4742 T7508 T7507 pobj plates,on
R4743 T7509 T7508 acl lacking,plates
R4744 T7510 T7509 dobj uracil,lacking
R4745 T7511 T7490 punct .,suppresses
R4746 T7513 T7514 compound LacZ,activation
R4747 T7514 T7515 nsubj activation,causes
R4748 T7516 T7517 compound X,gal
R4749 T7517 T7519 nsubj gal,turn
R4750 T7518 T7517 punct -,gal
R4751 T7519 T7515 ccomp turn,causes
R4752 T7520 T7519 aux to,turn
R4753 T7521 T7519 oprd blue,turn
R4754 T7522 T7521 prep in,blue
R4755 T7523 T7524 det a,assay
R4756 T7524 T7522 pobj assay,in
R4757 T7525 T7526 compound beta,galactosidase
R4758 T7526 T7524 compound galactosidase,assay
R4759 T7527 T7526 punct -,galactosidase
R4760 T7528 T7515 punct .,causes
R4761 T7530 T7531 det All,assays
R4762 T7531 T7532 nsubjpass assays,carried
R4763 T7533 T7532 auxpass were,carried
R4764 T7534 T7532 prt out,carried
R4765 T7535 T7532 prep in,carried
R4766 T7536 T7535 pobj parallel,in
R4767 T7537 T7536 prep with,parallel
R4768 T7538 T7539 det the,strains
R4769 T7539 T7537 pobj strains,with
R4770 T7540 T7539 nummod five,strains
R4771 T7541 T7539 compound ProQuest,strains
R4772 T7542 T7539 compound control,strains
R4773 T7543 T7539 compound yeast,strains
R4774 T7544 T7539 appos A,strains
R4775 T7545 T7546 punct -,E
R4776 T7546 T7544 prep E,A
R4777 T7547 T7539 punct ", ",strains
R4778 T7548 T7549 dep which,range
R4779 T7549 T7539 relcl range,strains
R4780 T7550 T7549 prep from,range
R4781 T7551 T7550 pobj non-interactor,from
R4782 T7552 T7553 punct (,A
R4783 T7553 T7551 parataxis A,non-interactor
R4784 T7554 T7553 punct ),A
R4785 T7555 T7550 prep to,from
R4786 T7556 T7557 amod strong,interactor
R4787 T7557 T7555 pobj interactor,to
R4788 T7558 T7559 punct (,E
R4789 T7559 T7557 parataxis E,interactor
R4790 T7560 T7559 punct ),E
R4791 T7561 T7532 punct .,carried
R4792 T7563 T7564 det All,procedures
R4793 T7564 T7565 nsubjpass procedures,carried
R4794 T7566 T7565 auxpass were,carried
R4795 T7567 T7565 prt out,carried
R4796 T7568 T7565 prep according,carried
R4797 T7569 T7568 prep to,according
R4798 T7570 T7571 det the,supplier
R4799 T7571 T7572 poss supplier,protocols
R4800 T7572 T7569 pobj protocols,to
R4801 T7573 T7571 case 's,supplier
R4802 T7574 T7565 punct .,carried
R4803 T7576 T7577 advmod Briefly,co-transformed
R4804 T7578 T7577 punct ", ",co-transformed
R4805 T7579 T7580 advmod chemically,competent
R4806 T7580 T7581 amod competent,cells
R4807 T7581 T7577 nsubjpass cells,co-transformed
R4808 T7582 T7581 compound MaV203,cells
R4809 T7583 T7581 compound yeast,cells
R4810 T7584 T7577 auxpass were,co-transformed
R4811 T7585 T7577 prep with,co-transformed
R4812 T7586 T7587 det the,library
R4813 T7587 T7585 pobj library,with
R4814 T7588 T7587 compound cDNA,library
R4815 T7589 T7587 cc and,library
R4816 T7590 T7591 det the,plasmid
R4817 T7591 T7587 conj plasmid,library
R4818 T7592 T7591 compound bait,plasmid
R4819 T7593 T7594 compound pDBLeu,PAX6CTP
R4820 T7594 T7591 appos PAX6CTP,plasmid
R4821 T7595 T7594 cc or,PAX6CTP
R4822 T7596 T7597 compound pDBLeu,PAX6PST
R4823 T7597 T7594 conj PAX6PST,PAX6CTP
R4824 T7598 T7577 punct .,co-transformed
R4825 T7600 T7601 nsubjpass Transformants,plated
R4826 T7602 T7603 punct (,107
R4827 T7603 T7600 parataxis 107,Transformants
R4828 T7604 T7603 nummod 5,107
R4829 T7605 T7603 punct ×,107
R4830 T7606 T7603 punct ),107
R4831 T7607 T7601 auxpass were,plated
R4832 T7608 T7601 prep on,plated
R4833 T7609 T7608 pobj medium,on
R4834 T7610 T7609 acl lacking,medium
R4835 T7611 T7610 dobj histidine,lacking
R4836 T7612 T7613 aux to,check
R4837 T7613 T7601 advcl check,plated
R4838 T7614 T7613 prep for,check
R4839 T7615 T7616 compound HIS3,activation
R4840 T7616 T7614 pobj activation,for
R4841 T7617 T7601 punct .,plated
R4842 T7619 T7620 compound HIS3,positives
R4843 T7620 T7621 nsubjpass positives,assayed
R4844 T7622 T7621 auxpass were,assayed
R4845 T7623 T7621 advmod then,assayed
R4846 T7624 T7621 prep for,assayed
R4847 T7625 T7626 det all,reporters
R4848 T7626 T7624 pobj reporters,for
R4849 T7627 T7626 nummod 3,reporters
R4850 T7628 T7621 punct .,assayed
R4851 T7630 T7631 det The,plasmid
R4852 T7631 T7634 nsubjpass plasmid,isolated
R4853 T7632 T7631 compound pPC86,plasmid
R4854 T7633 T7631 compound prey,plasmid
R4855 T7635 T7634 auxpass was,isolated
R4856 T7636 T7634 prep from,isolated
R4857 T7637 T7638 det all,positives
R4858 T7638 T7636 pobj positives,from
R4859 T7639 T7640 compound HIS3,LacZ
R4860 T7640 T7638 compound LacZ,positives
R4861 T7641 T7640 punct /,LacZ
R4862 T7642 T7640 compound URA3,LacZ
R4863 T7643 T7640 punct /,LacZ
R4864 T7644 T7634 cc and,isolated
R4865 T7645 T7646 det the,insert
R4866 T7646 T7648 nsubj insert,sequenced
R4867 T7647 T7646 compound cDNA,insert
R4868 T7648 T7634 conj sequenced,isolated
R4869 T7649 T7648 punct .,sequenced
R4870 T7651 T7652 compound BLAST,searches
R4871 T7652 T7653 nsubjpass searches,performed
R4872 T7654 T7653 auxpass were,performed
R4873 T7655 T7656 aux to,identify
R4874 T7656 T7653 advcl identify,performed
R4875 T7657 T7658 det the,insert
R4876 T7658 T7656 dobj insert,identify
R4877 T7659 T7658 compound cDNA,insert
R4878 T7660 T7661 punct [,24
R4879 T7661 T7653 parataxis 24,performed
R4880 T7662 T7661 punct ],24
R4881 T7663 T7653 punct .,performed

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7367 1524-1530 SO_EXT:0000667 denotes insert
T7366 1519-1523 SO_EXT:cDNA denotes cDNA
T7365 1455-1461 SO_EXT:0000667 denotes insert
T7364 1450-1454 SO_EXT:cDNA denotes cDNA
T7363 1427-1431 PR_EXT:000033987 denotes LacZ
T7362 1422-1426 PR_EXT:P03962 denotes URA3
T7361 1417-1421 PR_EXT:P06633 denotes HIS3
T7360 1387-1394 SO_EXT:0000155 denotes plasmid
T7359 1318-1322 PR_EXT:P06633 denotes HIS3
T7358 1301-1305 PR_EXT:P06633 denotes HIS3
T7357 1278-1287 CHEBI_SO_EXT:histidine denotes histidine
T7356 1224-1237 GO_EXT:transfectant_or_tranformant denotes Transformants
T7355 1182-1189 SO_EXT:0000155 denotes plasmid
T7354 1156-1160 SO_EXT:cDNA denotes cDNA
T7353 1135-1146 GO:0009294 denotes transformed
T7352 1121-1126 CL_GO_EXT:cell denotes cells
T7351 1115-1120 NCBITaxon_EXT:yeast denotes yeast
T7350 1087-1097 CHEBI_EXT:chemical_substance_or_polyatomic_entity denotes chemically
T7349 926-931 NCBITaxon_EXT:yeast denotes yeast
T7348 829-847 GO_EXT:0004565 denotes beta-galactosidase
T7347 805-810 CHEBI:75055 denotes X-gal
T7346 782-786 PR_EXT:000033987 denotes LacZ
T7345 774-780 CHEBI_EXT:uracil_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes uracil
T7344 749-755 GO_EXT:biological_growth_entity_or_process denotes growth
T7343 725-729 PR_EXT:P03962 denotes URA3
T7342 692-711 CHEBI:74498 denotes 5-fluro-orotic acid
T7341 664-670 GO_EXT:biological_growth_entity_or_process denotes growth
T7340 637-641 PR_EXT:P03962 denotes URA3
T7339 621-630 CHEBI_SO_EXT:histidine denotes histidine
T7338 596-602 GO_EXT:biological_growth_entity_or_process denotes growth
T7337 573-577 PR_EXT:P06633 denotes HIS3
T7336 558-562 PR_EXT:P04386 denotes GAL4
T7335 522-528 SO_EXT:0000417 denotes domain
T7334 506-510 PR_EXT:P04386 denotes GAL4
T7333 493-498 SO_EXT:sequence_fusion_process denotes fused
T7332 485-492 CHEBI_PR_EXT:protein denotes protein
T7331 445-451 SO_EXT:0000417 denotes domain
T7330 437-444 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T7329 433-436 CHEBI_SO_EXT:DNA denotes DNA
T7328 428-432 PR_EXT:P04386 denotes GAL4
T7327 415-420 SO_EXT:sequence_fusion_process denotes fused
T7326 407-414 CHEBI_PR_EXT:protein denotes protein
T7325 328-332 PR_EXT:000033987 denotes lacZ
T7324 319-323 PR_EXT:P03962 denotes URA3
T7323 313-317 PR_EXT:P06633 denotes HIS3
T7322 306-311 SO_EXT:0000704 denotes genes
T7321 282-286 PR_EXT:P04386 denotes GAL4
T7320 252-258 SO_EXT:0000417 denotes domain
T7319 236-240 PR_EXT:P04386 denotes GAL4
T7318 230-235 NCBITaxon_EXT:yeast denotes yeast
T7317 217-222 SO_EXT:sequence_fusion_process denotes fused
T7316 208-216 CHEBI_PR_EXT:protein denotes proteins
T7315 185-191 SO_EXT:0000440 denotes vector
T7314 132-142 SO_EXT:engineered_biological_sequence denotes constructs
T7313 47-51 SO_EXT:cDNA denotes cDNA
T7312 41-46 UBERON:0000955 denotes brain
T7311 35-40 NCBITaxon:10088 denotes mouse
T7310 0-5 NCBITaxon_EXT:yeast denotes Yeast

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7309 1524-1530 SO:0000667 denotes insert
T7308 1455-1461 SO:0000667 denotes insert
T7307 1427-1431 PR:000033987 denotes LacZ
T7306 1422-1426 PR:P03962 denotes URA3
T7305 1417-1421 PR:P06633 denotes HIS3
T7304 1387-1394 SO:0000155 denotes plasmid
T7303 1318-1322 PR:P06633 denotes HIS3
T7302 1301-1305 PR:P06633 denotes HIS3
T7301 1182-1189 SO:0000155 denotes plasmid
T7300 1135-1146 GO:0009294 denotes transformed
T7299 805-810 CHEBI:75055 denotes X-gal
T7298 782-786 PR:000033987 denotes LacZ
T7297 725-729 PR:P03962 denotes URA3
T7296 692-711 CHEBI:74498 denotes 5-fluro-orotic acid
T7295 637-641 PR:P03962 denotes URA3
T7294 573-577 PR:P06633 denotes HIS3
T7293 558-562 PR:P04386 denotes GAL4
T7292 522-528 SO:0000417 denotes domain
T7291 506-510 PR:P04386 denotes GAL4
T7290 445-451 SO:0000417 denotes domain
T7289 428-432 PR:P04386 denotes GAL4
T7288 328-332 PR:000033987 denotes lacZ
T7287 319-323 PR:P03962 denotes URA3
T7286 313-317 PR:P06633 denotes HIS3
T7285 306-311 SO:0000704 denotes genes
T7284 282-286 PR:P04386 denotes GAL4
T7283 252-258 SO:0000417 denotes domain
T7282 236-240 PR:P04386 denotes GAL4
T7281 185-191 SO:0000440 denotes vector
T7280 41-46 UBERON:0000955 denotes brain
T7279 35-40 NCBITaxon:10088 denotes mouse