PMC:1208879 / 25288-31045 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T8267	5756-5757	.	denotes	.
T8266	5755-5756	-RRB-	denotes	)
T8265	5748-5755	NNP	denotes	Promega
T8264	5747-5748	-LRB-	denotes	(
T8263	5734-5738	NN	denotes	size
T8262	5739-5746	NNS	denotes	markers
T8261	5726-5727	HYPH	denotes	/
T8260	5727-5733	NN	denotes	HaeIII
T8259	5721-5726	NN	denotes	ΦX174
T8258	5716-5720	IN	denotes	with
T8257	5704-5711	NN	denotes	agarose
T8256	5702-5703	NN	denotes	%
T8255	5699-5702	CD	denotes	2.5
T8254	5712-5715	NN	denotes	gel
T8253	5697-5698	DT	denotes	a
T8252	5694-5696	IN	denotes	on
T8251	5680-5684	VBD	denotes	were
T8250	5685-5693	VBN	denotes	resolved
T8249	5671-5679	NNS	denotes	Products
T8248	5670-5757	sentence	denotes	Products were resolved on a 2.5% agarose gel with ΦX174/HaeIII size markers (Promega).
T8247	5669-5670	.	denotes	.
T8246	5668-5669	CD	denotes	1
T8245	5666-5667	SYM	denotes	×
T8244	5664-5665	-RRB-	denotes	)
T8243	5661-5664	NN	denotes	min
T8242	5659-5660	CD	denotes	2
T8241	5655-5658	IN	denotes	for
T8240	5650-5652	CD	denotes	72
T8239	5652-5654	NN	denotes	°C
T8238	5649-5650	-LRB-	denotes	(
T8237	5645-5648	CC	denotes	and
T8236	5642-5644	CD	denotes	32
T8235	5640-5641	SYM	denotes	×
T8234	5638-5639	-RRB-	denotes	)
T8233	5635-5638	NN	denotes	sec
T8232	5632-5634	CD	denotes	30
T8231	5628-5631	IN	denotes	for
T8230	5625-5627	NN	denotes	°C
T8229	5623-5625	CD	denotes	72
T8228	5621-5623	,	denotes	,
T8227	5618-5621	NN	denotes	sec
T8226	5615-5617	CD	denotes	30
T8225	5611-5614	IN	denotes	for
T8224	5608-5610	NN	denotes	°C
T8223	5606-5608	CD	denotes	55
T8222	5604-5606	,	denotes	,
T8221	5601-5604	NN	denotes	sec
T8220	5598-5600	CD	denotes	30
T8219	5594-5597	IN	denotes	for
T8218	5589-5591	CD	denotes	94
T8217	5591-5593	NN	denotes	°C
T8216	5588-5589	-LRB-	denotes	(
T8215	5586-5588	,	denotes	,
T8214	5585-5586	CD	denotes	1
T8213	5583-5584	SYM	denotes	×
T8212	5581-5582	-RRB-	denotes	)
T8211	5578-5581	NN	denotes	sec
T8210	5575-5577	CD	denotes	30
T8209	5571-5574	IN	denotes	for
T8208	5568-5570	NN	denotes	°C
T8207	5566-5568	CD	denotes	95
T8206	5565-5566	-LRB-	denotes	(
T8205	5560-5564	VBD	denotes	were
T8204	5549-5559	NNS	denotes	conditions
T8203	5545-5548	NN	denotes	PCR
T8202	5544-5670	sentence	denotes	PCR conditions were (95°C for 30 sec) × 1, (94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec) × 32 and (72°C for 2 min) × 1.
T8201	5543-5544	.	denotes	.
T8200	5542-5543	-RRB-	denotes	)
T8199	5530-5536	NNP	denotes	Perkin
T8198	5537-5542	NNP	denotes	Elmer
T8197	5529-5530	-LRB-	denotes	(
T8196	5518-5528	NN	denotes	polymerase
T8195	5509-5517	NNP	denotes	AmpliTaq
T8194	5506-5508	IN	denotes	of
T8193	5500-5505	NNS	denotes	units
T8192	5496-5499	CD	denotes	2.5
T8191	5492-5495	CC	denotes	and
T8190	5480-5484	DT	denotes	each
T8189	5485-5491	NN	denotes	primer
T8188	5474-5479	NN	denotes	200μM
T8187	5472-5474	,	denotes	,
T8186	5467-5472	NN	denotes	MgCl2
T8185	5464-5466	NN	denotes	mM
T8184	5460-5463	CD	denotes	1.5
T8183	5458-5460	,	denotes	,
T8182	5457-5458	-RRB-	denotes	)
T8181	5445-5451	NNP	denotes	Perkin
T8180	5452-5457	NNP	denotes	Elmer
T8179	5444-5445	-LRB-	denotes	(
T8178	5426-5436	NN	denotes	polymerase
T8177	5417-5425	NNP	denotes	AmpliTaq
T8176	5416-5417	SYM	denotes	x
T8175	5437-5443	NN	denotes	buffer
T8174	5415-5416	CD	denotes	1
T8173	5404-5414	VBG	denotes	containing
T8172	5395-5403	NN	denotes	reaction
T8171	5393-5394	DT	denotes	a
T8170	5390-5392	IN	denotes	in
T8169	5375-5379	VBD	denotes	were
T8168	5380-5389	VBN	denotes	amplified
T8167	5362-5369	NN	denotes	library
T8166	5370-5374	NN	denotes	cDNA
T8165	5359-5361	NN	denotes	ng
T8164	5356-5358	CD	denotes	10
T8163	5355-5544	sentence	denotes	10 ng library cDNA were amplified in a reaction containing 1xAmpliTaq polymerase buffer (Perkin Elmer), 1.5 mM MgCl2, 200μM each primer and 2.5 units of AmpliTaq polymerase (Perkin Elmer).
T8162	5354-5355	.	denotes	.
T8161	5349-5352	NN	denotes	ATA
T8160	5345-5348	NN	denotes	GGG
T8159	5341-5344	NN	denotes	TCG
T8158	5337-5340	NN	denotes	GAT
T8157	5333-5336	NN	denotes	AGA
T8156	5353-5354	NN	denotes	A
T8155	5329-5332	NN	denotes	CAC
T8154	5327-5329	:	denotes	:
T8153	5325-5326	HYPH	denotes	-
T8152	5326-5327	NN	denotes	R
T8151	5319-5325	NN	denotes	Atp5a1
T8150	5317-5318	:	denotes	;
T8149	5311-5314	NN	denotes	CTC
T8148	5307-5310	NN	denotes	GTC
T8147	5303-5306	NN	denotes	GAT
T8146	5299-5302	NN	denotes	TGA
T8145	5295-5298	NN	denotes	ACG
T8144	5315-5317	NN	denotes	CA
T8143	5291-5294	NN	denotes	CAC
T8142	5289-5291	:	denotes	:
T8141	5287-5288	HYPH	denotes	-
T8140	5288-5289	NN	denotes	F
T8139	5281-5287	NN	denotes	Atp5a1
T8138	5279-5280	:	denotes	;
T8137	5273-5276	NN	denotes	GCC
T8136	5269-5272	NN	denotes	ACA
T8135	5265-5268	NN	denotes	CCC
T8134	5261-5264	NN	denotes	TTG
T8133	5257-5260	NN	denotes	ACC
T8132	5277-5279	NN	denotes	TT
T8131	5253-5256	NN	denotes	ATG
T8130	5251-5253	:	denotes	:
T8120	5240-5242	NN	denotes	GC
T8119	5216-5219	NN	denotes	CAT
T8118	5214-5216	:	denotes	:
T8117	5212-5213	HYPH	denotes	-
T8116	5213-5214	NN	denotes	F
T8115	5207-5212	NN	denotes	Gapdh
T8114	5205-5206	:	denotes	;
T8113	5198-5201	NN	denotes	ATG
T8112	5194-5197	NN	denotes	AAA
T8111	5190-5193	NN	denotes	AAA
T8110	5186-5189	NN	denotes	GAG
T8109	5182-5185	NN	denotes	AGG
T8108	5178-5181	NN	denotes	CTA
T8107	5202-5205	NN	denotes	GGG
T8106	5176-5177	NN	denotes	R
T8105	5175-5176	HYPH	denotes	-
T8104	5170-5175	NN	denotes	Dncl1
T8103	5168-5169	:	denotes	;
T8102	5163-5166	NNP	denotes	GTC
T8101	5159-5162	NNP	denotes	CAT
T8100	5155-5158	NNP	denotes	AGA
T8099	5151-5154	NN	denotes	TGC
T8098	5147-5150	NN	denotes	AAA
T8097	5143-5146	NN	denotes	CAA
T8096	5167-5168	NN	denotes	G
T8095	5141-5142	NN	denotes	F
T8094	5140-5141	HYPH	denotes	-
T8093	5135-5140	NN	denotes	Dncl1
T8092	5133-5134	:	denotes	;
T8091	5127-5130	NN	denotes	TAG
T8090	5123-5126	NN	denotes	TCA
T8089	5119-5122	NN	denotes	TAG
T8088	5115-5118	NN	denotes	AGA
T8087	5111-5114	NN	denotes	TGC
T8086	5107-5110	NN	denotes	GCC
T8085	5131-5133	NN	denotes	GG
T8084	5105-5106	NN	denotes	R
T8083	5104-5105	HYPH	denotes	-
T8082	5098-5104	NN	denotes	Trim11
T8081	5096-5097	:	denotes	;
T8080	5089-5092	NN	denotes	AAG
T8079	5085-5088	NN	denotes	GTG
T8078	5081-5084	NN	denotes	GAT
T8077	5077-5080	NN	denotes	CAG
T8076	5073-5076	NN	denotes	GTG
T8075	5093-5096	NN	denotes	CTG
T8074	5071-5072	NN	denotes	F
T8073	5070-5071	HYPH	denotes	-
T8072	5064-5070	NN	denotes	Trim11
T8071	5062-5063	:	denotes	;
T8070	5057-5060	NN	denotes	TCA
T8069	5053-5056	NN	denotes	AGC
T8068	5049-5052	NN	denotes	CAA
T8067	5045-5048	NN	denotes	GTG
T8066	5041-5044	NN	denotes	ACA
T8065	5037-5040	NN	denotes	TAC
T8064	5033-5036	NN	denotes	CTC
T8063	5061-5062	NN	denotes	G
T8062	5031-5032	NN	denotes	R
T8061	5030-5031	HYPH	denotes	-
T8060	5024-5030	NN	denotes	Homer3
T8059	5022-5023	:	denotes	;
T8058	5015-5018	NN	denotes	TAG
T8057	5011-5014	NN	denotes	CTG
T8056	5007-5010	NN	denotes	TGG
T8055	5003-5006	NN	denotes	AGG
T8054	4999-5002	NN	denotes	CCC
T8053	5019-5022	NN	denotes	AGC
T8052	4997-4998	NN	denotes	F
T8051	4996-4997	HYPH	denotes	-
T8050	4990-4996	NN	denotes	Homer3
T8049	4988-4989	:	denotes	;
T8048	4982-4985	NN	denotes	TCC
T8047	4978-4981	NN	denotes	CTC
T8046	4974-4977	NN	denotes	GCT
T8045	4970-4973	NN	denotes	CAT
T8044	4966-4969	NN	denotes	TCA
T8043	4962-4965	NN	denotes	CGA
T8042	4986-4988	NN	denotes	TT
T8041	4960-4961	NN	denotes	R
T8040	4959-4960	HYPH	denotes	-
T8039	4955-4959	NN	denotes	Pax6
T8038	4953-4954	:	denotes	;
T8037	4946-4949	NN	denotes	TAC
T8036	4942-4945	NN	denotes	ATC
T8035	4938-4941	NN	denotes	TAG
T8034	4934-4937	NN	denotes	AAA
T8033	4930-4933	NN	denotes	CCA
T8032	4926-4929	NN	denotes	CAG
T8031	4950-4953	NN	denotes	CTG
T8030	4924-4925	NN	denotes	F
T8029	4923-4924	HYPH	denotes	-
T8028	4919-4923	NN	denotes	Pax6
T8027	4917-4919	:	denotes	:
T8026	4913-4917	VBD	denotes	were
T8025	4903-4912	NNS	denotes	sequences
T8024	4896-4902	NN	denotes	Primer
T8023	4895-5355	sentence	denotes	Primer sequences were: Pax6-F CAG CCA AAA TAG ATC TAC CTG; Pax6-R CGA TCA CAT GCT CTC TCC TT; Homer3-F CCC AGG TGG CTG TAG AGC; Homer3-R CTC TAC ACA GTG CAA AGC TCA G; Trim11-F GTG CAG GAT GTG AAG CTG; Trim11-R GCC TGC AGA TAG TCA TAG GG; Dncl1-F CAA AAA TGC AGA CAT GTC G; Dncl1-R CTA AGG GAG AAA AAA ATG GGG; Gapdh-F: CAT CAC CAT CTT CCA GGA GC; Gapdh-R: ATG ACC TTG CCC ACA GCC TT; Atp5a1-F: CAC ACG TGA GAT GTC CTC CA; Atp5a1-R: CAC AGA GAT TCG GGG ATA A.
T8022	4894-4895	.	denotes	.
T8021	4880-4884	VBD	denotes	were
T8020	4865-4872	VBN	denotes	spliced
T8019	4855-4864	RB	denotes	correctly
T8018	4873-4879	NNS	denotes	clones
T8017	4850-4854	RB	denotes	only
T8016	4845-4849	IN	denotes	that
T8015	4885-4894	VBN	denotes	amplified
T8014	4842-4844	IN	denotes	so
T8013	4840-4842	,	denotes	,
T8012	4834-4840	NN	denotes	intron
T8011	4824-4829	RBS	denotes	least
T8010	4830-4833	CD	denotes	one
T8009	4821-4823	RB	denotes	at
T8008	4815-4820	VB	denotes	cross
T8007	4812-4814	TO	denotes	to
T8006	4798-4802	VBD	denotes	were
T8005	4803-4811	VBN	denotes	designed
T8004	4790-4797	NNS	denotes	Primers
T8003	4789-4895	sentence	denotes	Primers were designed to cross at least one intron, so that only correctly spliced clones were amplified.
T8002	4788-4789	.	denotes	.
T8001	4787-4788	-RRB-	denotes	]
T8000	4784-4785	,	denotes	,
T7999	4782-4784	CD	denotes	29
T7998	4785-4787	CD	denotes	30
T7997	4781-4782	-LRB-	denotes	[
T7996	4774-4780	NN	denotes	Atp5a1
T7995	4770-4773	CC	denotes	and
T7994	4764-4769	NN	denotes	Gapdh
T7993	4748-4757	VBN	denotes	expressed
T7992	4733-4747	RB	denotes	constitutively
T7991	4758-4763	NNS	denotes	genes
T7990	4729-4732	DT	denotes	the
T7989	4725-4728	CC	denotes	and
T7988	4718-4724	NN	denotes	Trim11
T7987	4716-4718	,	denotes	,
T7986	4711-4716	NN	denotes	Dncl1
T7985	4709-4711	,	denotes	,
T7984	4703-4709	NN	denotes	Homer3
T7983	4701-4703	,	denotes	,
T7982	4697-4701	NN	denotes	Pax6
T7981	4694-4696	IN	denotes	on
T7980	4680-4683	VBD	denotes	was
T7979	4676-4679	NN	denotes	PCR
T7978	4658-4675	JJ	denotes	semi-quantitative
T7977	4656-4658	,	denotes	,
T7976	4644-4648	NN	denotes	cDNA
T7975	4649-4656	NN	denotes	library
T7974	4640-4643	DT	denotes	the
T7973	4637-4639	IN	denotes	in
T7972	4630-4636	NNS	denotes	clones
T7971	4627-4629	IN	denotes	of
T7970	4603-4611	JJ	denotes	relative
T7969	4612-4626	NN	denotes	representation
T7968	4599-4602	DT	denotes	the
T7967	4684-4693	VBN	denotes	performed
T7966	4593-4598	VB	denotes	check
T7965	4590-4592	TO	denotes	To
T7964	4589-4789	sentence	denotes	To check the relative representation of clones in the cDNA library, semi-quantitative PCR was performed on Pax6, Homer3, Dncl1, Trim11 and the constitutively expressed genes Gapdh and Atp5a1 [29,30].
T7963	4586-4589	NN	denotes	PCR
T7962	4568-4585	JJ	denotes	Semi-quantitative
T7843	4565-4566	.	denotes	.
T7842	4545-4555	NN	denotes	expression
T7841	4537-4538	HYPH	denotes	-
T7840	4538-4544	NN	denotes	length
T7839	4533-4537	JJ	denotes	full
T7838	4556-4565	NN	denotes	construct
T7837	4531-4532	DT	denotes	a
T7836	4524-4530	VB	denotes	create
T7835	4521-4523	TO	denotes	to
T7834	4505-4506	HYPH	denotes	-
T7833	4506-4512	NN	denotes	Homer3
T7832	4500-4505	NN	denotes	pPC86
T7831	4513-4520	NN	denotes	plasmid
T7830	4496-4499	DT	denotes	the
T7829	4491-4495	IN	denotes	into
T7828	4482-4490	VBN	denotes	inserted
T7827	4478-4481	CC	denotes	and
T7826	4474-4477	NN	denotes	PCR
T7825	4471-4473	IN	denotes	by
T7824	4459-4464	NN	denotes	IMAGE
T7823	4465-4470	NN	denotes	clone
T7822	4455-4458	DT	denotes	the
T7821	4450-4454	IN	denotes	from
T7820	4436-4439	VBD	denotes	was
T7819	4440-4449	VBN	denotes	amplified
T7818	4419-4426	JJ	denotes	missing
T7817	4427-4435	NN	denotes	fragment
T7816	4415-4418	DT	denotes	The
T7815	4414-4566	sentence	denotes	The missing fragment was amplified from the IMAGE clone by PCR and inserted into the pPC86-Homer3 plasmid to create a full-length expression construct.
T7814	4413-4414	.	denotes	.
T7813	4405-4413	NNP	denotes	Research
T7812	4396-4404	NNP	denotes	Genomics
T7811	4392-4395	IN	denotes	for
T7810	4376-4384	NNP	denotes	Franklin
T7809	4367-4375	NNP	denotes	Rosalind
T7808	4385-4391	NNP	denotes	Centre
T7807	4363-4366	DT	denotes	the
T7806	4358-4362	IN	denotes	from
T7805	4349-4357	VBN	denotes	obtained
T7804	4345-4348	CC	denotes	and
T7803	4327-4335	NN	denotes	sequence
T7802	4316-4326	NN	denotes	nucleotide
T7801	4312-4315	NN	denotes	EST
T7800	4336-4344	NN	denotes	database
T7799	4308-4311	DT	denotes	the
T7798	4305-4307	IN	denotes	of
T7797	4292-4297	NNP	denotes	BLAST
T7796	4298-4304	NN	denotes	search
T7795	4290-4291	DT	denotes	a
T7794	4287-4289	IN	denotes	by
T7793	4272-4275	VBD	denotes	was
T7792	4260-4265	NN	denotes	amino
T7791	4257-4259	CD	denotes	70
T7790	4247-4248	HYPH	denotes	-
T7789	4248-4256	JJ	denotes	terminal
T7788	4246-4247	NN	denotes	N
T7787	4238-4245	JJ	denotes	missing
T7786	4266-4271	NNS	denotes	acids
T7785	4234-4237	DT	denotes	the
T7784	4223-4233	VBG	denotes	containing
T7783	4221-4222	-RRB-	denotes	)
T7782	4206-4212	NN	denotes	number
T7781	4213-4221	NN	denotes	BE569374
T7780	4196-4205	NN	denotes	accession
T7779	4194-4196	,	denotes	,
T7778	4187-4194	CD	denotes	3602414
T8129	5249-5250	HYPH	denotes	-
T8128	5250-5251	NN	denotes	R
T8127	5244-5249	NN	denotes	Gapdh
T8126	5242-5243	:	denotes	;
T8125	5236-5239	NN	denotes	GGA
T8124	5232-5235	NN	denotes	CCA
T8123	5228-5231	NN	denotes	CTT
T8122	5224-5227	NN	denotes	CAT
T8121	5220-5223	NN	denotes	CAC
T7777	4175-4180	NN	denotes	IMAGE
T7776	4181-4186	NN	denotes	clone
T7775	4174-4175	-LRB-	denotes	(
T7774	4163-4167	NN	denotes	cDNA
T7773	4168-4173	NN	denotes	clone
T7772	4161-4162	DT	denotes	a
T7771	4159-4161	,	denotes	,
T7770	4154-4159	NNS	denotes	tests
T7769	4145-4153	JJ	denotes	pairwise
T7768	4141-4144	IN	denotes	for
T7767	4128-4134	NN	denotes	Homer3
T7766	4120-4121	HYPH	denotes	-
T7765	4121-4127	NN	denotes	length
T7764	4116-4120	JJ	denotes	full
T7763	4135-4140	NN	denotes	clone
T7762	4114-4115	DT	denotes	a
T7761	4276-4286	VBN	denotes	identified
T7760	4107-4113	VB	denotes	create
T7759	4104-4106	TO	denotes	To
T7758	4103-4414	sentence	denotes	To create a full-length Homer3 clone for pairwise tests, a cDNA clone (IMAGE clone 3602414, accession number BE569374) containing the missing N-terminal 70 amino acids was identified by a BLAST search of the EST nucleotide sequence database and obtained from the Rosalind Franklin Centre for Genomics Research.
T7757	4102-4103	.	denotes	.
T7756	4096-4102	RB	denotes	before
T7755	4093-4095	IN	denotes	as
T7754	4078-4082	NN	denotes	LacZ
T7753	4074-4077	CC	denotes	and
T7752	4069-4073	NN	denotes	URA3
T7751	4067-4069	,	denotes	,
T7750	4063-4067	NN	denotes	HIS3
T7749	4083-4092	NNS	denotes	reporters
T7748	4059-4062	DT	denotes	the
T7747	4056-4058	IN	denotes	of
T7746	4045-4055	NN	denotes	activation
T7745	4041-4044	IN	denotes	for
T7744	4022-4031	VBG	denotes	resulting
T7743	4032-4040	NNS	denotes	colonies
T7742	4018-4021	DT	denotes	the
T7741	4010-4017	VBG	denotes	testing
T7740	4006-4009	CC	denotes	and
T7739	4004-4005	-RRB-	denotes	)
T7738	3999-4004	NN	denotes	pPC86
T7737	3996-3998	IN	denotes	in
T7736	3995-3996	-LRB-	denotes	(
T7735	3985-3994	NN	denotes	construct
T7734	3980-3984	NN	denotes	prey
T7733	3976-3979	CD	denotes	one
T7732	3972-3975	CC	denotes	and
T7731	3970-3971	-RRB-	denotes	)
T7730	3964-3970	NN	denotes	pDBLeu
T7729	3961-3963	IN	denotes	in
T7728	3960-3961	-LRB-	denotes	(
T7727	3950-3959	NN	denotes	construct
T7726	3945-3949	NN	denotes	bait
T7725	3941-3944	CD	denotes	one
T7724	3936-3940	IN	denotes	with
T7723	3924-3929	NN	denotes	yeast
T7722	3917-3923	NN	denotes	MaV203
T7721	3930-3935	NNS	denotes	cells
T7720	3907-3916	JJ	denotes	competent
T7719	3894-3906	VBG	denotes	transforming
T7718	3891-3893	IN	denotes	by
T7717	3879-3883	VBD	denotes	were
T7716	3884-3890	VBN	denotes	tested
T7715	3866-3878	NNS	denotes	interactions
T7714	3857-3865	JJ	denotes	Specific
T7713	3856-4103	sentence	denotes	Specific interactions were tested by transforming competent MaV203 yeast cells with one bait construct (in pDBLeu) and one prey construct (in pPC86) and testing the resulting colonies for activation of the HIS3, URA3 and LacZ reporters as before.
T7712	3844-3856	NNS	denotes	interactions
T7711	3835-3843	JJ	denotes	Pairwise
T7663	3832-3833	.	denotes	.
T7662	3831-3832	-RRB-	denotes	]
T7661	3829-3831	CD	denotes	24
T7660	3828-3829	-LRB-	denotes	[
T7659	3816-3820	NN	denotes	cDNA
T7486	2928-3078	sentence	denotes	Weak URA3 activation suppresses growth on plates containing 5-fluro-orotic acid while strong URA3 activation permits growth on plates lacking uracil.
T7485	2927-2928	.	denotes	.
T7484	2918-2927	NN	denotes	histidine
T7483	2910-2917	VBG	denotes	lacking
T7482	2903-2909	NNS	denotes	plates
T7481	2900-2902	IN	denotes	on
T7480	2893-2899	NN	denotes	growth
T7479	2886-2892	VBZ	denotes	allows
T7478	2875-2885	NN	denotes	activation
T7477	2870-2874	NN	denotes	HIS3
T7476	2869-2928	sentence	denotes	HIS3 activation allows growth on plates lacking histidine.
T7475	2868-2869	.	denotes	.
T7474	2860-2868	NN	denotes	function
T7473	2855-2859	NN	denotes	GAL4
T7472	2840-2854	VBG	denotes	reconstituting
T7471	2835-2839	RB	denotes	thus
T7470	2833-2835	,	denotes	,
T7469	2832-2833	-RRB-	denotes	)
T7468	2827-2832	NN	denotes	pPC86
T7467	2826-2827	-LRB-	denotes	(
T7466	2808-2818	NN	denotes	activation
T7465	2803-2807	NN	denotes	GAL4
T7464	2819-2825	NN	denotes	domain
T7463	2799-2802	DT	denotes	the
T7462	2796-2798	IN	denotes	to
T7461	2790-2795	VBN	denotes	fused
T7460	2777-2781	NN	denotes	prey
T7459	2782-2789	NN	denotes	protein
T7458	2773-2776	DT	denotes	the
T7457	2768-2772	IN	denotes	with
T7456	2756-2757	-RRB-	denotes	)
T7455	2750-2756	NN	denotes	pDBLeu
T7454	2749-2750	-LRB-	denotes	(
T7453	2734-2741	NN	denotes	binding
T7452	2730-2733	NN	denotes	DNA
T7451	2725-2729	NN	denotes	GAL4
T7450	2742-2748	NN	denotes	domain
T7449	2721-2724	DT	denotes	the
T7448	2718-2720	IN	denotes	to
T7447	2712-2717	VBN	denotes	fused
T7446	2699-2703	NN	denotes	bait
T7445	2704-2711	NN	denotes	protein
T7444	2695-2698	DT	denotes	the
T7443	2758-2767	VBZ	denotes	interacts
T7442	2690-2694	WRB	denotes	when
T7441	2676-2679	VBP	denotes	are
T7440	2680-2689	VBN	denotes	activated
T7439	2666-2675	NNS	denotes	Reporters
T7438	2665-2869	sentence	denotes	Reporters are activated when the bait protein fused to the GAL4 DNA binding domain (pDBLeu) interacts with the prey protein fused to the GAL4 activation domain (pPC86), thus reconstituting GAL4 function.
T7437	2664-2665	.	denotes	.
T7436	2652-2664	NNS	denotes	interactions
T7435	2643-2651	JJ	denotes	positive
T7434	2634-2642	VB	denotes	identify
T7433	2631-2633	TO	denotes	to
T7432	2629-2631	,	denotes	,
T7431	2625-2629	NN	denotes	lacZ
T7430	2621-2624	CC	denotes	and
T7429	2616-2620	NN	denotes	URA3
T7428	2614-2616	,	denotes	,
T7427	2610-2614	NN	denotes	HIS3
T7426	2608-2610	,	denotes	,
T7425	2594-2602	NN	denotes	reporter
T7424	2583-2584	HYPH	denotes	-
T7423	2584-2593	VBN	denotes	activated
T7422	2579-2583	NN	denotes	GAL4
T7421	2603-2608	NNS	denotes	genes
T7420	2573-2578	CD	denotes	three
T7419	2568-2572	VBZ	denotes	uses
T7418	2561-2567	NN	denotes	system
T7417	2557-2560	DT	denotes	The
T7416	2556-2665	sentence	denotes	The system uses three GAL4-activated reporter genes, HIS3, URA3 and lacZ, to identify positive interactions.
T7415	2555-2556	.	denotes	.
T7414	2538-2548	NN	denotes	activation
T7413	2533-2537	NN	denotes	GAL4
T7412	2527-2532	NN	denotes	yeast
T7411	2549-2555	NN	denotes	domain
T7410	2523-2526	DT	denotes	the
T7409	2520-2522	IN	denotes	to
T7408	2514-2519	VBN	denotes	fused
T7407	2505-2513	NN	denotes	proteins
T7406	2496-2504	VBZ	denotes	produces
T7405	2490-2495	WDT	denotes	which
T7404	2488-2490	,	denotes	,
T7403	2476-2481	NN	denotes	pPC86
T7402	2482-2488	NN	denotes	vector
T7401	2472-2475	DT	denotes	the
T7400	2469-2471	IN	denotes	in
T7399	2453-2456	VBD	denotes	was
T7398	2457-2468	VBN	denotes	constructed
T7397	2445-2452	NN	denotes	library
T7396	2441-2444	DT	denotes	The
T7395	2440-2556	sentence	denotes	The library was constructed in the pPC86 vector, which produces proteins fused to the yeast GAL4 activation domain.
T7394	2439-2440	.	denotes	.
T7393	2422-2428	NN	denotes	pDBLeu
T7392	2414-2421	NN	denotes	PAX6CTP
T7391	2410-2413	CC	denotes	and
T7390	2402-2409	NN	denotes	PAX6PST
T7389	2429-2439	NNS	denotes	constructs
T7388	2398-2401	DT	denotes	the
T7387	2393-2397	IN	denotes	with
T7386	2380-2383	VBD	denotes	was
T7385	2378-2379	-RRB-	denotes	)
T7384	2368-2378	NNP	denotes	Invitrogen
T7383	2366-2368	,	denotes	,
T7382	2358-2366	NNP	denotes	ProQuest
T7381	2357-2358	-LRB-	denotes	(
T7380	2384-2392	VBN	denotes	screened
T7379	2344-2348	NN	denotes	cDNA
T7378	2338-2343	NN	denotes	brain
T7377	2332-2337	NN	denotes	mouse
T7376	2349-2356	NN	denotes	library
T7375	2330-2331	DT	denotes	A
T7374	2329-2440	sentence	denotes	A mouse brain cDNA library (ProQuest, Invitrogen) was screened with the PAX6PST and PAX6CTP pDBLeu constructs.
T7373	2314-2321	NN	denotes	library
T7372	2306-2307	HYPH	denotes	-
T7371	2307-2313	NN	denotes	hybrid
T7370	2303-2306	CD	denotes	two
T7369	2322-2329	NNS	denotes	screens
T7368	2297-2302	NN	denotes	Yeast
T6664	409-410	.	denotes	.
T6663	408-409	-RRB-	denotes	]
T6662	405-406	,	denotes	,
T6661	403-405	CD	denotes	25
T6660	406-408	CD	denotes	26
T6659	402-403	-LRB-	denotes	[
T6658	385-394	NN	denotes	consensus
T6657	379-384	NN	denotes	JPRED
T6656	395-401	NN	denotes	method
T6655	375-378	DT	denotes	the
T6654	369-374	VBG	denotes	using
T6653	355-358	VBD	denotes	was
T6652	359-368	VBN	denotes	performed
T6651	344-354	NN	denotes	prediction
T6650	334-343	NN	denotes	structure
T6649	324-333	JJ	denotes	Secondary
T6648	323-410	sentence	denotes	Secondary structure prediction was performed using the JPRED consensus method [25,26].
T6647	322-323	.	denotes	.
T6646	321-322	-RRB-	denotes	]
T6645	319-321	CD	denotes	24
T6644	318-319	-LRB-	denotes	[
T6643	310-317	NNP	denotes	CLUSTAL
T6642	304-309	VBG	denotes	using
T6641	291-295	VBD	denotes	were
T6640	286-290	NN	denotes	PAX6
T6639	280-285	JJ	denotes	human
T6638	277-279	IN	denotes	of
T6637	267-268	HYPH	denotes	-
T6636	266-267	NN	denotes	C
T6635	268-276	NN	denotes	terminus
T6634	262-265	DT	denotes	the
T6633	259-261	IN	denotes	to
T6632	248-258	JJ	denotes	homologous
T6631	241-247	RB	denotes	highly
T6630	236-240	VBD	denotes	were
T6629	231-235	WDT	denotes	that
T6628	296-303	VBN	denotes	aligned
T6627	221-230	NNS	denotes	sequences
T6626	213-220	NN	denotes	Protein
T6625	212-323	sentence	denotes	Protein sequences that were highly homologous to the C-terminus of human PAX6 were aligned using CLUSTAL [24].
T6624	211-212	.	denotes	.
T6623	210-211	-RRB-	denotes	]
T7658	3821-3827	NN	denotes	insert
T7657	3812-3815	DT	denotes	the
T7656	3803-3811	VB	denotes	identify
T7655	3800-3802	TO	denotes	to
T7654	3785-3789	VBD	denotes	were
T7653	3790-3799	VBN	denotes	performed
T7652	3776-3784	NNS	denotes	searches
T7651	3770-3775	NNP	denotes	BLAST
T7650	3769-3833	sentence	denotes	BLAST searches were performed to identify the cDNA insert [24].
T7649	3768-3769	.	denotes	.
T7648	3759-3768	VBN	denotes	sequenced
T7647	3747-3751	NN	denotes	cDNA
T7646	3752-3758	NN	denotes	insert
T7645	3743-3746	DT	denotes	the
T7644	3739-3742	CC	denotes	and
T7643	3723-3724	HYPH	denotes	/
T7642	3719-3723	NN	denotes	URA3
T7641	3718-3719	HYPH	denotes	/
T7640	3724-3728	NN	denotes	LacZ
T7639	3714-3718	NN	denotes	HIS3
T7638	3729-3738	NNS	denotes	positives
T7637	3710-3713	DT	denotes	all
T7636	3705-3709	IN	denotes	from
T7635	3692-3695	VBD	denotes	was
T7634	3696-3704	VBN	denotes	isolated
T7633	3679-3683	NN	denotes	prey
T7632	3673-3678	NN	denotes	pPC86
T7631	3684-3691	NN	denotes	plasmid
T7630	3669-3672	DT	denotes	The
T7629	3668-3769	sentence	denotes	The pPC86 prey plasmid was isolated from all HIS3/URA3/LacZ positives and the cDNA insert sequenced.
T7628	3667-3668	.	denotes	.
T7627	3656-3657	CD	denotes	3
T7626	3658-3667	NNS	denotes	reporters
T7625	3652-3655	DT	denotes	all
T7624	3648-3651	IN	denotes	for
T7623	3635-3639	RB	denotes	then
T7622	3630-3634	VBD	denotes	were
T7621	3640-3647	VBN	denotes	assayed
T7620	3620-3629	NNS	denotes	positives
T7619	3615-3619	NN	denotes	HIS3
T7618	3614-3668	sentence	denotes	HIS3 positives were then assayed for all 3 reporters.
T7617	3613-3614	.	denotes	.
T7616	3603-3613	NN	denotes	activation
T7615	3598-3602	NN	denotes	HIS3
T7614	3594-3597	IN	denotes	for
T7613	3588-3593	VB	denotes	check
T7612	3585-3587	TO	denotes	to
T7611	3575-3584	NN	denotes	histidine
T7610	3567-3574	VBG	denotes	lacking
T7609	3560-3566	NN	denotes	medium
T7608	3557-3559	IN	denotes	on
T7607	3545-3549	VBD	denotes	were
T7606	3543-3544	-RRB-	denotes	)
T7605	3538-3539	SYM	denotes	×
T7604	3536-3537	CD	denotes	5
T7603	3540-3543	CD	denotes	107
T7602	3535-3536	-LRB-	denotes	(
T7601	3550-3556	VBN	denotes	plated
T7600	3521-3534	NNS	denotes	Transformants
T7599	3520-3614	sentence	denotes	Transformants (5 × 107) were plated on medium lacking histidine to check for HIS3 activation.
T7598	3519-3520	.	denotes	.
T7597	3512-3519	NN	denotes	PAX6PST
T7596	3505-3511	NN	denotes	pDBLeu
T7595	3502-3504	CC	denotes	or
T7594	3494-3501	NN	denotes	PAX6CTP
T7593	3487-3493	NN	denotes	pDBLeu
T7592	3474-3478	NN	denotes	bait
T7591	3479-3486	NN	denotes	plasmid
T7590	3470-3473	DT	denotes	the
T7589	3466-3469	CC	denotes	and
T7588	3453-3457	NN	denotes	cDNA
T7587	3458-3465	NN	denotes	library
T7586	3449-3452	DT	denotes	the
T7585	3444-3448	IN	denotes	with
T7584	3424-3428	VBD	denotes	were
T7583	3412-3417	NN	denotes	yeast
T7582	3405-3411	NN	denotes	MaV203
T7581	3418-3423	NNS	denotes	cells
T7580	3395-3404	JJ	denotes	competent
T7579	3384-3394	RB	denotes	chemically
T7578	3382-3384	,	denotes	,
T7577	3429-3443	VBN	denotes	co-transformed
T7576	3375-3382	RB	denotes	Briefly
T7575	3374-3520	sentence	denotes	Briefly, chemically competent MaV203 yeast cells were co-transformed with the cDNA library and the bait plasmid pDBLeu PAX6CTP or pDBLeu PAX6PST.
T7574	3373-3374	.	denotes	.
T7573	3361-3363	POS	denotes	's
T7572	3364-3373	NNS	denotes	protocols
T7571	3353-3361	NN	denotes	supplier
T7570	3349-3352	DT	denotes	the
T7569	3346-3348	IN	denotes	to
T7568	3336-3345	VBG	denotes	according
T7567	3332-3335	RP	denotes	out
T7566	3319-3323	VBD	denotes	were
T7565	3324-3331	VBN	denotes	carried
T7564	3308-3318	NNS	denotes	procedures
T7563	3304-3307	DT	denotes	All
T7562	3303-3374	sentence	denotes	All procedures were carried out according to the supplier's protocols.
T7561	3302-3303	.	denotes	.
T7560	3301-3302	-RRB-	denotes	)
T7559	3300-3301	NN	denotes	E
T7558	3299-3300	-LRB-	denotes	(
T7557	3288-3298	NN	denotes	interactor
T7556	3281-3287	JJ	denotes	strong
T7555	3278-3280	IN	denotes	to
T7554	3276-3277	-RRB-	denotes	)
T7553	3275-3276	NN	denotes	A
T7552	3274-3275	-LRB-	denotes	(
T7551	3259-3273	NN	denotes	non-interactor
T7550	3254-3258	IN	denotes	from
T7549	3248-3253	VBP	denotes	range
T7548	3242-3247	WDT	denotes	which
T7547	3240-3242	,	denotes	,
T7546	3239-3240	NN	denotes	E
T7545	3238-3239	SYM	denotes	-
T7544	3237-3238	NN	denotes	A
T7543	3223-3228	NN	denotes	yeast
T7542	3215-3222	NN	denotes	control
T7541	3206-3214	NNP	denotes	ProQuest
T7540	3201-3205	CD	denotes	five
T7539	3229-3236	NNS	denotes	strains
T7538	3197-3200	DT	denotes	the
T7537	3192-3196	IN	denotes	with
T7536	3183-3191	NN	denotes	parallel
T7535	3180-3182	IN	denotes	in
T7534	3176-3179	RP	denotes	out
T7533	3163-3167	VBD	denotes	were
T7532	3168-3175	VBN	denotes	carried
T7531	3156-3162	NNS	denotes	assays
T7530	3152-3155	DT	denotes	All
T7529	3151-3303	sentence	denotes	All assays were carried out in parallel with the five ProQuest control yeast strains A-E, which range from non-interactor (A) to strong interactor (E).
T7528	3150-3151	.	denotes	.
T7527	3130-3131	HYPH	denotes	-
T7526	3131-3144	NN	denotes	galactosidase
T7525	3126-3130	NN	denotes	beta
T7524	3145-3150	NN	denotes	assay
T7523	3124-3125	DT	denotes	a
T7522	3121-3123	IN	denotes	in
T7521	3116-3120	JJ	denotes	blue
T7520	3108-3110	TO	denotes	to
T7519	3111-3115	VB	denotes	turn
T7518	3103-3104	HYPH	denotes	-
T7517	3104-3107	NN	denotes	gal
T7516	3102-3103	NN	denotes	X
T7515	3095-3101	VBZ	denotes	causes
T7514	3084-3094	NN	denotes	activation
T7513	3079-3083	NN	denotes	LacZ
T7512	3078-3151	sentence	denotes	LacZ activation causes X-gal to turn blue in a beta-galactosidase assay.
T7511	3077-3078	.	denotes	.
T7510	3071-3077	NN	denotes	uracil
T7509	3063-3070	VBG	denotes	lacking
T7508	3056-3062	NNS	denotes	plates
T7507	3053-3055	IN	denotes	on
T7506	3046-3052	NN	denotes	growth
T7505	3022-3026	NN	denotes	URA3
T7504	3027-3037	NN	denotes	activation
T7503	3015-3021	JJ	denotes	strong
T7502	3038-3045	VBZ	denotes	permits
T7501	3009-3014	IN	denotes	while
T7500	3004-3008	NN	denotes	acid
T7499	2996-2997	HYPH	denotes	-
T7498	2991-2996	JJ	denotes	fluro
T7497	2990-2991	HYPH	denotes	-
T7496	2997-3003	JJ	denotes	orotic
T7495	2989-2990	CD	denotes	5
T7494	2978-2988	VBG	denotes	containing
T7493	2971-2977	NNS	denotes	plates
T7492	2968-2970	IN	denotes	on
T7491	2961-2967	NN	denotes	growth
T7490	2950-2960	VBZ	denotes	suppresses
T7489	2934-2938	NN	denotes	URA3
T7488	2939-2949	NN	denotes	activation
T7487	2929-2933	JJ	denotes	Weak
T6622	208-210	CD	denotes	24
T6621	207-208	-LRB-	denotes	[
T6620	198-206	NNP	denotes	Research
T6619	189-197	NNP	denotes	Genomics
T6618	185-188	IN	denotes	for
T6617	169-177	NNP	denotes	Franklin
T6616	160-168	NNP	denotes	Rosalind
T6615	178-184	NNP	denotes	Centre
T6614	156-159	DT	denotes	the
T6613	153-155	IN	denotes	at
T6612	125-139	NNP	denotes	Bioinformatics
T6611	140-152	NNP	denotes	Applications
T6610	121-124	DT	denotes	the
T6609	113-120	IN	denotes	through
T6608	103-112	JJ	denotes	available
T6607	89-94	NNP	denotes	BLAST
T6606	95-102	NN	denotes	program
T6605	85-88	DT	denotes	the
T6604	79-84	VBG	denotes	using
T6603	75-78	RP	denotes	out
T6602	62-66	VBD	denotes	were
T6601	67-74	VBN	denotes	carried
T6600	44-52	NN	denotes	database
T6599	53-61	NNS	denotes	searches
T6598	35-43	NN	denotes	Sequence
T6597	34-212	sentence	denotes	Sequence database searches were carried out using the BLAST program available through the Bioinformatics Applications at the Rosalind Franklin Centre for Genomics Research [24].
T6596	24-34	NNS	denotes	techniques
T6595	9-23	NN	denotes	Bioinformatics
T7133	1815-1821	NN	denotes	mutant
T7132	1833-1845	NN	denotes	substitution
T7131	1811-1814	DT	denotes	the
T7130	1802-1810	VBZ	denotes	contains
T7129	1796-1801	WDT	denotes	which
T7128	1794-1796	,	denotes	,
T7127	1793-1794	SYM	denotes	'
T7126	1792-1793	CD	denotes	3
T7125	1791-1792	HYPH	denotes	-
T7124	1785-1788	NN	denotes	TTG
T7123	1781-1784	NN	denotes	GTA
T7122	1777-1780	NN	denotes	CCA
T7121	1773-1776	NN	denotes	TGG
T7120	1769-1772	NN	denotes	TCT
T7119	1765-1768	NN	denotes	TAA
T7118	1761-1764	NN	denotes	CCG
T7117	1757-1760	NN	denotes	TTA
T7116	1753-1756	NN	denotes	TTT
T7115	1749-1752	NN	denotes	CGC
T7114	1745-1748	NN	denotes	GGC
T7113	1741-1744	NN	denotes	TGC
T7112	1737-1740	NN	denotes	TTT
T7111	1736-1737	HYPH	denotes	-
T7110	1735-1736	SYM	denotes	'
T7109	1734-1735	CD	denotes	5
T7108	1789-1791	NN	denotes	AG
T7107	1728-1733	NN	denotes	ST006
T7106	1717-1720	NN	denotes	PCR
T7105	1709-1716	JJ	denotes	reverse
T7104	1721-1727	NN	denotes	primer
T7103	1705-1708	DT	denotes	the
T7102	1699-1704	VBG	denotes	using
T7101	1695-1698	CC	denotes	but
T7100	1693-1695	,	denotes	,
T7099	1676-1683	NN	denotes	PAX6PST
T7098	1684-1693	NN	denotes	construct
T7097	1672-1675	DT	denotes	the
T7096	1669-1671	IN	denotes	as
T7095	1660-1664	JJ	denotes	same
T7094	1665-1668	NN	denotes	way
T7093	1656-1659	DT	denotes	the
T7092	1653-1655	IN	denotes	in
T7091	1639-1642	VBD	denotes	was
T7090	1637-1638	-RRB-	denotes	)
T7089	1632-1637	NN	denotes	Q422R
T7088	1631-1632	-LRB-	denotes	(
T7087	1629-1630	NN	denotes	G
T7086	1628-1629	SYM	denotes	>
T7085	1623-1628	NN	denotes	1627A
T7084	1614-1622	NN	denotes	mutation
T7083	1610-1613	DT	denotes	the
T7082	1599-1609	VBG	denotes	containing
T7081	1643-1652	VBN	denotes	generated
T7080	1578-1581	NN	denotes	PST
T7079	1582-1588	NN	denotes	domain
T7078	1573-1577	NN	denotes	PAX6
T7077	1589-1598	NN	denotes	construct
T7076	1571-1572	DT	denotes	A
T7075	1570-1859	sentence	denotes	A PAX6 PST domain construct containing the mutation 1627A>G (Q422R) was generated in the same way as the PAX6PST construct, but using the reverse PCR primer ST006 5'-TTT TGC GGC CGC TTT TTA CCG TAA TCT TGG CCA GTA TTG AG-3', which contains the mutant nucleotide substitution (underlined).
T7074	1569-1570	.	denotes	.
T7073	1554-1558	VBN	denotes	been
T7072	1550-1553	VBD	denotes	had
T7071	1543-1549	NNS	denotes	errors
T7070	1540-1542	DT	denotes	no
T7069	1559-1569	VBN	denotes	introduced
T7068	1535-1539	IN	denotes	that
T7067	1529-1534	VB	denotes	check
T7066	1526-1528	TO	denotes	to
T7065	1511-1515	VBD	denotes	were
T7064	1503-1510	NNS	denotes	linkers
T7063	1498-1502	IN	denotes	with
T7062	1495-1497	CC	denotes	or
T7061	1491-1494	NN	denotes	PCR
T7060	1488-1490	IN	denotes	by
T7059	1478-1487	VBN	denotes	generated
T7058	1516-1525	VBN	denotes	sequenced
T7057	1468-1477	NNS	denotes	fragments
T7056	1464-1467	DT	denotes	All
T7055	1463-1570	sentence	denotes	All fragments generated by PCR or with linkers were sequenced to check that no errors had been introduced.
T7054	1462-1463	.	denotes	.
T7053	1445-1456	NN	denotes	restriction
T7052	1441-1444	CD	denotes	two
T7051	1457-1462	NNS	denotes	sites
T7050	1437-1440	DT	denotes	the
T7049	1429-1436	IN	denotes	between
T7048	1412-1421	JJ	denotes	synthetic
T7047	1422-1428	NN	denotes	linker
T7046	1410-1411	DT	denotes	a
T7045	1400-1409	VBG	denotes	inserting
T7044	1396-1399	CC	denotes	and
T7043	1394-1396	,	denotes	,
T7042	1377-1378	HYPH	denotes	-
T7041	1378-1386	JJ	denotes	terminal
T7040	1376-1377	NN	denotes	C
T7039	1387-1394	NN	denotes	peptide
T7038	1372-1375	DT	denotes	the
T7037	1368-1371	RP	denotes	out
T7036	1363-1367	VB	denotes	drop
T7035	1360-1362	TO	denotes	to
T7034	1355-1359	NN	denotes	NotI
T7033	1351-1354	CC	denotes	and
T7032	1346-1350	NN	denotes	NdeI
T7031	1341-1345	IN	denotes	with
T7030	1333-1340	NN	denotes	PAX6PST
T7029	1325-1332	VBG	denotes	cutting
T7028	1322-1324	IN	denotes	by
T7027	1313-1316	VBD	denotes	was
T7026	1317-1321	VBN	denotes	made
T7025	1308-1312	DT	denotes	This
T7024	1307-1463	sentence	denotes	This was made by cutting PAX6PST with NdeI and NotI to drop out the C-terminal peptide, and inserting a synthetic linker between the two restriction sites.
T7023	1306-1307	.	denotes	.
T7022	1305-1306	-RRB-	denotes	)
T7021	1302-1305	CD	denotes	390
T7020	1301-1302	SYM	denotes	–
T7019	1298-1301	CD	denotes	278
T7018	1297-1298	-LRB-	denotes	(
T7017	1279-1280	HYPH	denotes	-
T7016	1280-1288	JJ	denotes	terminal
T7015	1278-1279	NN	denotes	C
T7014	1289-1296	NN	denotes	peptide
T7013	1274-1277	DT	denotes	the
T7012	1268-1273	CC	denotes	minus
T7011	1257-1260	NN	denotes	PST
T7010	1261-1267	NN	denotes	domain
T7009	1253-1256	DT	denotes	the
T7008	1244-1252	VBZ	denotes	contains
T7007	1240-1241	HYPH	denotes	-
T7006	1241-1243	NN	denotes	CT
T7005	1233-1240	NN	denotes	PAX6PST
T7004	1232-1307	sentence	denotes	PAX6PST-CT contains the PST domain minus the C-terminal peptide (278–390).
T7003	1231-1232	.	denotes	.
T7002	1230-1231	-RRB-	denotes	)
T7001	1229-1230	NN	denotes	R
T7000	1228-1229	-LRB-	denotes	(
T6999	1222-1227	NN	denotes	ST005
T6998	1218-1221	CC	denotes	and
T6997	1216-1217	SYM	denotes	'
T6996	1215-1216	CD	denotes	3
T6995	1214-1215	HYPH	denotes	-
T6994	1208-1211	NN	denotes	TCA
T6993	1204-1207	NN	denotes	GAC
T6992	1200-1203	NN	denotes	CAG
T6991	1196-1199	NN	denotes	CAA
T6990	1192-1195	NN	denotes	CTT
T6989	1188-1191	NN	denotes	CCA
T6988	1184-1187	NN	denotes	CTA
T6987	1180-1183	NN	denotes	CGA
T6986	1176-1179	NN	denotes	AGT
T6985	1172-1175	NN	denotes	AAA
T6984	1171-1172	HYPH	denotes	-
T6983	1170-1171	SYM	denotes	'
T6982	1169-1170	CD	denotes	5
T6981	1167-1168	-RRB-	denotes	)
T6980	1166-1167	NN	denotes	F
T6979	1165-1166	-LRB-	denotes	(
T6978	1212-1214	NN	denotes	TT
T6977	1159-1164	NN	denotes	ST004
T6976	1154-1158	VBD	denotes	were
T6975	1146-1153	NNS	denotes	Primers
T6974	1145-1232	sentence	denotes	Primers were ST004 (F) 5'-AAA AGT CGA CTA CCA CTT CAA CAG GAC TCA TT-3' and ST005 (R).
T6973	1144-1145	.	denotes	.
T6972	1143-1144	-RRB-	denotes	)
T6971	1140-1143	CD	denotes	422
T6970	1139-1140	SYM	denotes	–
T6969	1136-1139	CD	denotes	391
T6968	1135-1136	-LRB-	denotes	(
T6967	1129-1134	JJ	denotes	alone
T6966	1111-1112	HYPH	denotes	-
T6965	1112-1120	JJ	denotes	terminal
T6964	1110-1111	NN	denotes	C
T6963	1102-1109	VBN	denotes	defined
T6962	1096-1101	RB	denotes	newly
T6961	1121-1128	NN	denotes	peptide
T6960	1092-1095	DT	denotes	the
T6959	1083-1091	VBZ	denotes	contains
T6958	1075-1082	NN	denotes	PAX6CTP
T6957	1074-1145	sentence	denotes	PAX6CTP contains the newly defined C-terminal peptide alone (391–422).
T6956	1073-1074	.	denotes	.
T6955	1072-1073	SYM	denotes	'
T6954	1071-1072	CD	denotes	3
T6953	1070-1071	HYPH	denotes	-
T6952	1063-1066	NN	denotes	GTA
T6951	1059-1062	NN	denotes	CCA
T6950	1055-1058	NN	denotes	TGG
T6949	1051-1054	NN	denotes	TCT
T6948	1047-1050	NN	denotes	TAA
T6947	1043-1046	NN	denotes	CTG
T6946	1039-1042	NN	denotes	TTA
T6945	1035-1038	NN	denotes	TTT
T6944	1031-1034	NN	denotes	GGC
T6943	1027-1030	NN	denotes	TGC
T6942	1023-1026	NN	denotes	TTT
T6941	1022-1023	HYPH	denotes	-
T6940	1021-1022	SYM	denotes	'
T6939	1020-1021	CD	denotes	5
T6938	1018-1019	-RRB-	denotes	)
T6937	1017-1018	NN	denotes	R
T6936	1016-1017	-LRB-	denotes	(
T6935	1067-1070	NN	denotes	TTG
T6934	1010-1015	NN	denotes	ST005
T6933	1006-1009	CC	denotes	and
T6932	1004-1005	SYM	denotes	'
T6931	1003-1004	CD	denotes	3
T6930	1002-1003	HYPH	denotes	-
T6928	993-996	NN	denotes	CCT
T6927	989-992	NN	denotes	ACA
T6926	985-988	NN	denotes	AAC
T6925	981-984	NN	denotes	AGC
T6924	977-980	NN	denotes	GCC
T6923	973-976	NN	denotes	ACT
T6922	969-972	NN	denotes	TCG
T6921	965-968	NN	denotes	AGT
T6920	961-964	NN	denotes	AAA
T6919	960-961	HYPH	denotes	-
T6918	959-960	SYM	denotes	'
T6909	928-1074	sentence	denotes	Primers were ST001 (forward) 5'-AAA AGT TCG ACT GCC AGC AAC ACA CCT AGT C-3' and ST005 (R) 5'-TTT TGC GGC TTT TTA CTG TAA TCT TGG CCA GTA TTG-3'.
T6908	927-928	.	denotes	.
T6907	926-927	-RRB-	denotes	)
T6906	914-918	NN	denotes	PAX6
T6905	906-907	HYPH	denotes	-
T6904	907-913	NN	denotes	length
T6903	902-906	JJ	denotes	full
T6902	919-926	NN	denotes	protein
T6901	898-901	DT	denotes	the
T6900	895-897	IN	denotes	of
T6899	891-894	CD	denotes	422
T6898	890-891	SYM	denotes	–
T6897	881-886	NNS	denotes	acids
T6896	875-880	NN	denotes	amino
T6895	887-890	CD	denotes	278
T6894	874-875	-LRB-	denotes	(
T6893	863-866	NN	denotes	PST
T6892	857-862	JJ	denotes	whole
T6891	867-873	NN	denotes	domain
T6890	853-856	DT	denotes	the
T6889	844-852	VBZ	denotes	contains
T6888	836-843	NN	denotes	PAX6PST
T6887	835-928	sentence	denotes	PAX6PST contains the whole PST domain (amino acids 278–422 of the full-length PAX6 protein).
T6886	834-835	.	denotes	.
T6885	820-827	NN	denotes	binding
T6884	816-819	NN	denotes	DNA
T6883	811-815	NN	denotes	GAL4
T6882	805-810	NN	denotes	yeast
T6881	828-834	NN	denotes	domain
T6880	801-804	DT	denotes	the
T6879	798-800	IN	denotes	to
T6878	792-797	VBN	denotes	fused
T6877	784-791	NN	denotes	protein
T6876	782-783	DT	denotes	a
T6875	772-781	VBZ	denotes	generates
T6874	766-771	WDT	denotes	which
T6873	764-766	,	denotes	,
T6872	763-764	-RRB-	denotes	)
T6871	753-763	NNP	denotes	Invitrogen
T6870	751-753	,	denotes	,
T6869	737-738	HYPH	denotes	-
T6868	738-744	NN	denotes	Hybrid
T6867	734-737	CD	denotes	Two
T6866	725-733	NNP	denotes	ProQuest
T6865	745-751	NN	denotes	System
T6864	724-725	-LRB-	denotes	(
T6863	706-716	NN	denotes	expression
T6862	699-705	NN	denotes	pDBLeu
T6861	717-723	NN	denotes	vector
T6860	695-698	DT	denotes	the
T6859	692-694	IN	denotes	in
T6858	676-680	NN	denotes	cDNA
T6857	671-675	NN	denotes	PAX6
T6856	681-691	NNS	denotes	constructs
T6855	665-670	CD	denotes	three
T6854	660-664	VB	denotes	make
T6853	657-659	TO	denotes	to
T6852	647-651	VBD	denotes	were
T6851	652-656	VBN	denotes	used
T6850	625-635	NN	denotes	subcloning
T6849	621-624	CC	denotes	and
T6848	617-620	NN	denotes	PCR
T6847	636-646	NNS	denotes	techniques
T6846	608-616	JJ	denotes	Standard
T6845	607-835	sentence	denotes	Standard PCR and subcloning techniques were used to make three PAX6 cDNA constructs in the pDBLeu expression vector (ProQuest Two-Hybrid System, Invitrogen), which generates a protein fused to the yeast GAL4 DNA binding domain.
T6844	606-607	.	denotes	.
T6843	605-606	-RRB-	denotes	]
T6842	603-605	CD	denotes	27
T6841	602-603	-LRB-	denotes	[
T6840	593-596	NN	denotes	web
T6839	576-583	NN	denotes	Variant
T6838	568-575	JJ	denotes	Allelic
T6837	584-592	NN	denotes	Database
T6836	563-567	NN	denotes	PAX6
T6835	557-562	JJ	denotes	Human
T6834	597-601	NN	denotes	site
T6833	553-556	DT	denotes	the
T6832	548-552	IN	denotes	from
T6831	538-547	JJ	denotes	available
T6830	518-527	NN	denotes	reference
T6829	510-517	NN	denotes	protein
T6828	506-509	CC	denotes	and
T6827	496-500	NN	denotes	PAX6
T6826	501-505	NN	denotes	cDNA
T6825	490-495	JJ	denotes	human
T6824	528-537	NNS	denotes	sequences
T6823	486-489	DT	denotes	the
T6822	483-485	IN	denotes	on
T6821	477-482	VBN	denotes	based
T6820	474-476	VBZ	denotes	is
T6819	459-463	NN	denotes	acid
T6818	453-458	NN	denotes	amino
T6817	449-452	CC	denotes	and
T6816	444-448	NN	denotes	cDNA
T6815	464-473	NN	denotes	numbering
T6814	440-443	DT	denotes	All
T6813	439-607	sentence	denotes	All cDNA and amino acid numbering is based on the human PAX6 cDNA and protein reference sequences available from the Human PAX6 Allelic Variant Database web site [27].
T6812	421-422	HYPH	denotes	-
T6811	422-428	NN	denotes	hybrid
T6810	418-421	CD	denotes	two
T6809	429-439	NNS	denotes	constructs
T6808	412-417	NN	denotes	Yeast
T7154	1934-1935	-RRB-	denotes	)
T7247	2294-2295	.	denotes	.
T7246	2282-2285	VBD	denotes	was
T7245	2286-2294	VBN	denotes	inserted
T7244	2266-2272	NN	denotes	mutant
T7243	2273-2281	NN	denotes	sequence
T7242	2262-2265	DT	denotes	the
T7241	2258-2261	CC	denotes	and
T7240	2256-2258	,	denotes	,
T7239	2239-2240	HYPH	denotes	-
T7238	2240-2248	JJ	denotes	terminal
T7237	2238-2239	NN	denotes	C
T7236	2231-2237	JJ	denotes	normal
T7235	2249-2256	NN	denotes	peptide
T7234	2227-2230	DT	denotes	the
T7233	2219-2226	VB	denotes	release
T7232	2216-2218	TO	denotes	to
T7231	2211-2215	NN	denotes	NotI
T7230	2207-2210	CC	denotes	and
T7229	2202-2206	NN	denotes	NdeI
T7228	2197-2201	IN	denotes	with
T7227	2189-2192	VBD	denotes	was
T7226	2193-2196	VBN	denotes	cut
T7225	2171-2178	NN	denotes	PAX6PST
T7224	2179-2188	NN	denotes	construct
T7223	2167-2170	DT	denotes	The
T7222	2166-2295	sentence	denotes	The PAX6PST construct was cut with NdeI and NotI to release the normal C-terminal peptide, and the mutant sequence was inserted.
T7221	2165-2166	.	denotes	.
T7220	2164-2165	SYM	denotes	'
T7219	2163-2164	CD	denotes	3
T7218	2162-2163	HYPH	denotes	-
T7217	2156-2159	NN	denotes	TGT
T7216	2152-2155	NN	denotes	CAT
T7215	2148-2151	NN	denotes	CTA
T7214	2144-2147	NN	denotes	AGT
T7213	2140-2143	NN	denotes	TCC
T7212	2136-2139	NN	denotes	CCA
T7211	2132-2135	NN	denotes	CAT
T7210	2128-2131	NN	denotes	CCG
T7209	2124-2127	NN	denotes	CGG
T7208	2120-2123	NN	denotes	TTG
T7207	2119-2120	HYPH	denotes	-
T7206	2118-2119	SYM	denotes	'
T7205	2117-2118	CD	denotes	5
T7204	2115-2116	-RRB-	denotes	)
T7203	2114-2115	NN	denotes	R
T7202	2113-2114	-LRB-	denotes	(
T7201	2160-2162	NN	denotes	TC
T7200	2107-2112	NN	denotes	ST031
T7199	2103-2106	CC	denotes	and
T7198	2101-2102	SYM	denotes	'
T7197	2100-2101	CD	denotes	3
T7196	2099-2100	HYPH	denotes	-
T7195	2094-2097	NN	denotes	ACA
T7194	2090-2093	NN	denotes	GAC
T7193	2086-2089	NN	denotes	GCA
T7192	2082-2085	NN	denotes	TAT
T7191	2078-2081	NN	denotes	ACA
T7190	2074-2077	NN	denotes	CCC
T7189	2073-2074	HYPH	denotes	-
T7188	2072-2073	SYM	denotes	'
T7187	2071-2072	CD	denotes	5
T7186	2069-2070	-RRB-	denotes	)
T7185	2068-2069	NN	denotes	F
T7184	2067-2068	-LRB-	denotes	(
T7183	2098-2099	NN	denotes	C
T7182	2061-2066	NN	denotes	ST015
T7181	2056-2060	VBD	denotes	were
T7180	2048-2055	NNS	denotes	Primers
T7179	2047-2166	sentence	denotes	Primers were ST015 (F) 5'-CCC ACA TAT GCA GAC ACA C-3' and ST031 (R) 5'-TTG CGG CCG CAT CCA TCC AGT CTA CAT TGT TC-3'.
T7178	2046-2047	.	denotes	.
T7177	2045-2046	-RRB-	denotes	)
T7176	2032-2035	NNP	denotes	van
T7175	2030-2031	NNP	denotes	V
T7174	2036-2045	NNP	denotes	Heyningen
T7173	2025-2029	NNP	denotes	Prof
T7172	2021-2024	CC	denotes	and
T7171	2008-2009	NNP	denotes	K
T7170	2010-2020	NNP	denotes	Williamson
T7169	2005-2007	NNP	denotes	Dr
T7168	2000-2004	IN	denotes	from
T7167	1993-1994	DT	denotes	a
T7166	1995-1999	NN	denotes	gift
T7165	1992-1993	-LRB-	denotes	(
T7164	1988-1991	NN	denotes	RNA
T7163	1976-1987	VBN	denotes	transcribed
T7162	1968-1975	JJ	denotes	reverse
T7161	1963-1967	IN	denotes	from
T7160	1959-1962	NN	denotes	PCR
T7159	1956-1958	IN	denotes	by
T7158	1941-1945	VBD	denotes	were
T7157	1939-1940	-RRB-	denotes	]
T7156	1937-1939	CD	denotes	12
T7155	1936-1937	-LRB-	denotes	[
T7153	1929-1934	NN	denotes	X423L
T7152	1928-1929	-LRB-	denotes	(
T7151	1919-1927	NN	denotes	1629insT
T7150	1915-1918	CC	denotes	and
T7149	1913-1914	-RRB-	denotes	]
T7148	1911-1913	CD	denotes	28
T7147	1910-1911	-LRB-	denotes	[
T7146	1900-1909	NN	denotes	1615del10
T7145	1890-1899	NNS	denotes	mutations
T7144	1886-1889	DT	denotes	the
T7143	1875-1885	VBG	denotes	containing
T7142	1946-1955	VBN	denotes	generated
T7141	1865-1874	NNS	denotes	sequences
T7140	1860-1864	NN	denotes	cDNA
T7139	1859-2047	sentence	denotes	cDNA sequences containing the mutations 1615del10 [28] and 1629insT (X423L) [12] were generated by PCR from reverse transcribed RNA (a gift from Dr K Williamson and Prof V van Heyningen).
T7138	1858-1859	.	denotes	.
T7137	1857-1858	-RRB-	denotes	)
T7136	1847-1857	JJ	denotes	underlined
T7135	1846-1847	-LRB-	denotes	(
T7134	1822-1832	NN	denotes	nucleotide
T6917	958-959	CD	denotes	5
T6916	956-957	-RRB-	denotes	)
T6915	949-956	JJ	denotes	forward
T6914	948-949	-LRB-	denotes	(
T6913	1001-1002	NN	denotes	C
T6912	942-947	NN	denotes	ST001
T6911	937-941	VBD	denotes	were
T6910	929-936	NNS	denotes	Primers
T6929	997-1000	NN	denotes	AGT
R4137	T6595	T6596	compound	Bioinformatics,techniques
R4138	T6598	T6599	compound	Sequence,searches
R4139	T6599	T6601	nsubjpass	searches,carried
R4140	T6600	T6599	compound	database,searches
R4141	T6602	T6601	auxpass	were,carried
R4142	T6603	T6601	prt	out,carried
R4143	T6604	T6601	advcl	using,carried
R4144	T6605	T6606	det	the,program
R4145	T6606	T6604	dobj	program,using
R4146	T6607	T6606	compound	BLAST,program
R4147	T6608	T6606	amod	available,program
R4148	T6609	T6608	prep	through,available
R4149	T6610	T6611	det	the,Applications
R4150	T6611	T6609	pobj	Applications,through
R4151	T6612	T6611	compound	Bioinformatics,Applications
R4152	T6613	T6611	prep	at,Applications
R4153	T6614	T6615	det	the,Centre
R4154	T6615	T6613	pobj	Centre,at
R4155	T6616	T6617	compound	Rosalind,Franklin
R4156	T6617	T6615	compound	Franklin,Centre
R4157	T6618	T6615	prep	for,Centre
R4158	T6619	T6620	compound	Genomics,Research
R4159	T6620	T6618	pobj	Research,for
R4160	T6621	T6622	punct	[,24
R4161	T6622	T6601	parataxis	24,carried
R4162	T6623	T6622	punct	],24
R4163	T6624	T6601	punct	.,carried
R4164	T6626	T6627	compound	Protein,sequences
R4165	T6627	T6628	nsubjpass	sequences,aligned
R4166	T6629	T6630	dep	that,were
R4167	T6630	T6627	relcl	were,sequences
R4168	T6631	T6632	advmod	highly,homologous
R4169	T6632	T6630	acomp	homologous,were
R4170	T6633	T6632	prep	to,homologous
R4171	T6634	T6635	det	the,terminus
R4172	T6635	T6633	pobj	terminus,to
R4173	T6636	T6635	compound	C,terminus
R4174	T6637	T6635	punct	-,terminus
R4175	T6638	T6635	prep	of,terminus
R4176	T6639	T6640	amod	human,PAX6
R4177	T6640	T6638	pobj	PAX6,of
R4178	T6641	T6628	auxpass	were,aligned
R4179	T6642	T6628	advcl	using,aligned
R4180	T6643	T6642	dobj	CLUSTAL,using
R4181	T6644	T6645	punct	[,24
R4182	T6645	T6628	parataxis	24,aligned
R4183	T6646	T6645	punct	],24
R4184	T6647	T6628	punct	.,aligned
R4185	T6649	T6650	amod	Secondary,structure
R4186	T6650	T6651	compound	structure,prediction
R4187	T6651	T6652	nsubjpass	prediction,performed
R4188	T6653	T6652	auxpass	was,performed
R4189	T6654	T6652	advcl	using,performed
R4190	T6655	T6656	det	the,method
R4191	T6656	T6654	dobj	method,using
R4192	T6657	T6656	compound	JPRED,method
R4193	T6658	T6656	compound	consensus,method
R4194	T6659	T6660	punct	[,26
R4195	T6660	T6652	parataxis	26,performed
R4196	T6661	T6660	nummod	25,26
R4197	T6662	T6660	punct	",",26
R4198	T6663	T6660	punct	],26
R4199	T6664	T6652	punct	.,performed
R4202	T6808	T6809	nmod	Yeast,constructs
R4203	T6810	T6811	nummod	two,hybrid
R4204	T6811	T6809	compound	hybrid,constructs
R4205	T6812	T6811	punct	-,hybrid
R4206	T6814	T6815	det	All,numbering
R4207	T6815	T6820	nsubj	numbering,is
R4208	T6816	T6815	nmod	cDNA,numbering
R4209	T6817	T6816	cc	and,cDNA
R4210	T6818	T6819	compound	amino,acid
R4211	T6819	T6816	conj	acid,cDNA
R4212	T6821	T6820	prep	based,is
R4213	T6822	T6821	prep	on,based
R4214	T6823	T6824	det	the,sequences
R4215	T6824	T6822	pobj	sequences,on
R4216	T6825	T6826	amod	human,cDNA
R4217	T6826	T6824	nmod	cDNA,sequences
R4218	T6827	T6826	nmod	PAX6,cDNA
R4219	T6828	T6826	cc	and,cDNA
R4220	T6829	T6830	compound	protein,reference
R4221	T6830	T6826	conj	reference,cDNA
R4222	T6831	T6824	amod	available,sequences
R4223	T6832	T6831	prep	from,available
R4224	T6833	T6834	det	the,site
R4225	T6834	T6832	pobj	site,from
R4226	T6835	T6836	amod	Human,PAX6
R4227	T6836	T6837	nmod	PAX6,Database
R4228	T6837	T6834	compound	Database,site
R4229	T6838	T6839	amod	Allelic,Variant
R4230	T6839	T6837	compound	Variant,Database
R4231	T6840	T6834	compound	web,site
R4232	T6841	T6842	punct	[,27
R4233	T6842	T6821	parataxis	27,based
R4234	T6843	T6842	punct	],27
R4235	T6844	T6820	punct	.,is
R4236	T6846	T6847	amod	Standard,techniques
R4237	T6847	T6851	nsubjpass	techniques,used
R4238	T6848	T6847	nmod	PCR,techniques
R4239	T6849	T6848	cc	and,PCR
R4240	T6850	T6848	conj	subcloning,PCR
R4241	T6852	T6851	auxpass	were,used
R4242	T6853	T6854	aux	to,make
R4243	T6854	T6851	advcl	make,used
R4244	T6855	T6856	nummod	three,constructs
R4245	T6856	T6854	dobj	constructs,make
R4246	T6857	T6858	compound	PAX6,cDNA
R4247	T6858	T6856	compound	cDNA,constructs
R4248	T6859	T6854	prep	in,make
R4249	T6860	T6861	det	the,vector
R4250	T6861	T6859	pobj	vector,in
R4251	T6862	T6861	compound	pDBLeu,vector
R4252	T6863	T6861	compound	expression,vector
R4253	T6864	T6865	punct	(,System
R4254	T6865	T6861	parataxis	System,vector
R4255	T6866	T6865	nmod	ProQuest,System
R4256	T6867	T6868	nummod	Two,Hybrid
R4257	T6868	T6865	compound	Hybrid,System
R4258	T6869	T6868	punct	-,Hybrid
R4259	T6870	T6865	punct	", ",System
R4260	T6871	T6865	npadvmod	Invitrogen,System
R4261	T6872	T6865	punct	),System
R4262	T6873	T6861	punct	", ",vector
R4263	T6874	T6875	dep	which,generates
R4264	T6875	T6861	relcl	generates,vector
R4265	T6876	T6877	det	a,protein
R4266	T6877	T6875	dobj	protein,generates
R4267	T6878	T6877	acl	fused,protein
R4268	T6879	T6878	prep	to,fused
R4269	T6880	T6881	det	the,domain
R4270	T6881	T6879	pobj	domain,to
R4271	T6882	T6881	compound	yeast,domain
R4272	T6883	T6881	compound	GAL4,domain
R4273	T6884	T6885	compound	DNA,binding
R4274	T6885	T6881	compound	binding,domain
R4275	T6886	T6851	punct	.,used
R4276	T6888	T6889	nsubj	PAX6PST,contains
R4277	T6890	T6891	det	the,domain
R4278	T6891	T6889	dobj	domain,contains
R4279	T6892	T6891	amod	whole,domain
R4280	T6893	T6891	compound	PST,domain
R4281	T6894	T6895	punct	(,278
R4282	T6895	T6889	parataxis	278,contains
R4283	T6896	T6895	nmod	amino,278
R4284	T6897	T6895	nmod	acids,278
R4285	T6898	T6899	punct	–,422
R4286	T6899	T6895	prep	422,278
R4287	T6900	T6895	prep	of,278
R4288	T6901	T6902	det	the,protein
R4289	T6902	T6900	pobj	protein,of
R4290	T6903	T6904	amod	full,length
R4291	T6904	T6902	compound	length,protein
R4292	T6905	T6904	punct	-,length
R4293	T6906	T6902	compound	PAX6,protein
R4294	T6907	T6895	punct	),278
R4295	T6908	T6889	punct	.,contains
R4296	T6910	T6911	nsubj	Primers,were
R4297	T6912	T6913	nmod	ST001,C
R4298	T6913	T6911	attr	C,were
R4299	T6914	T6915	punct	(,forward
R4300	T6915	T6913	parataxis	forward,C
R4301	T6916	T6915	punct	),forward
R4302	T6917	T6913	nummod	5,C
R4303	T6918	T6917	punct	',5
R4304	T6919	T6913	punct	-,C
R4305	T6920	T6913	compound	AAA,C
R4306	T6921	T6913	compound	AGT,C
R4307	T6922	T6913	compound	TCG,C
R4308	T6923	T6913	compound	ACT,C
R4309	T6924	T6913	compound	GCC,C
R4310	T6925	T6913	compound	AGC,C
R4311	T6926	T6913	compound	AAC,C
R4312	T6927	T6913	compound	ACA,C
R4313	T6928	T6913	compound	CCT,C
R4314	T6929	T6913	compound	AGT,C
R4315	T6930	T6913	punct	-,C
R4316	T6931	T6913	nummod	3,C
R4317	T6932	T6913	punct	',C
R4318	T6933	T6913	cc	and,C
R4319	T6934	T6935	nmod	ST005,TTG
R4320	T6935	T6913	conj	TTG,C
R4321	T6936	T6937	punct	(,R
R4322	T6937	T6935	parataxis	R,TTG
R4323	T6938	T6937	punct	),R
R4324	T6939	T6935	nummod	5,TTG
R4325	T6940	T6939	punct	',5
R4326	T6941	T6935	punct	-,TTG
R4327	T6942	T6935	compound	TTT,TTG
R4328	T6943	T6935	compound	TGC,TTG
R4329	T6944	T6935	compound	GGC,TTG
R4330	T6945	T6935	compound	TTT,TTG
R4331	T6946	T6935	compound	TTA,TTG
R4332	T6947	T6935	compound	CTG,TTG
R4333	T6948	T6935	compound	TAA,TTG
R4334	T6949	T6935	compound	TCT,TTG
R4335	T6950	T6935	compound	TGG,TTG
R4336	T6951	T6935	compound	CCA,TTG
R4337	T6952	T6935	compound	GTA,TTG
R4338	T6953	T6935	punct	-,TTG
R4339	T6954	T6935	nummod	3,TTG
R4340	T6955	T6935	punct	',TTG
R4341	T6956	T6911	punct	.,were
R4342	T6958	T6959	nsubj	PAX6CTP,contains
R4343	T6960	T6961	det	the,peptide
R4344	T6961	T6959	dobj	peptide,contains
R4345	T6962	T6963	advmod	newly,defined
R4346	T6963	T6961	amod	defined,peptide
R4347	T6964	T6965	npadvmod	C,terminal
R4348	T6965	T6961	amod	terminal,peptide
R4349	T6966	T6965	punct	-,terminal
R4350	T6967	T6961	amod	alone,peptide
R4351	T6968	T6969	punct	(,391
R4352	T6969	T6959	parataxis	391,contains
R4353	T6970	T6971	punct	–,422
R4354	T6971	T6969	prep	422,391
R4355	T6972	T6969	punct	),391
R4356	T6973	T6959	punct	.,contains
R4357	T6975	T6976	nsubj	Primers,were
R4358	T6977	T6978	nmod	ST004,TT
R4359	T6978	T6976	attr	TT,were
R4360	T6979	T6980	punct	(,F
R4361	T6980	T6978	parataxis	F,TT
R4362	T6981	T6980	punct	),F
R4363	T6982	T6978	nummod	5,TT
R4364	T6983	T6982	punct	',5
R4365	T6984	T6978	punct	-,TT
R4366	T6985	T6978	compound	AAA,TT
R4367	T6986	T6978	compound	AGT,TT
R4368	T6987	T6978	compound	CGA,TT
R4369	T6988	T6978	compound	CTA,TT
R4370	T6989	T6978	compound	CCA,TT
R4371	T6990	T6978	compound	CTT,TT
R4372	T6991	T6978	compound	CAA,TT
R4373	T6992	T6978	compound	CAG,TT
R4374	T6993	T6978	compound	GAC,TT
R4375	T6994	T6978	compound	TCA,TT
R4376	T6995	T6978	punct	-,TT
R4377	T6996	T6978	nummod	3,TT
R4378	T6997	T6978	punct	',TT
R4379	T6998	T6978	cc	and,TT
R4380	T6999	T6978	conj	ST005,TT
R4381	T7000	T7001	punct	(,R
R4382	T7001	T6999	parataxis	R,ST005
R4383	T7002	T7001	punct	),R
R4384	T7003	T6976	punct	.,were
R4385	T7005	T7006	compound	PAX6PST,CT
R4386	T7006	T7008	nsubj	CT,contains
R4387	T7007	T7006	punct	-,CT
R4388	T7009	T7010	det	the,domain
R4389	T7010	T7008	dobj	domain,contains
R4390	T7011	T7010	compound	PST,domain
R4391	T7012	T7010	cc	minus,domain
R4392	T7013	T7014	det	the,peptide
R4393	T7014	T7010	conj	peptide,domain
R4394	T7015	T7016	npadvmod	C,terminal
R4395	T7016	T7014	amod	terminal,peptide
R4396	T7017	T7016	punct	-,terminal
R4397	T7018	T7019	punct	(,278
R4398	T7019	T7008	parataxis	278,contains
R4399	T7020	T7021	punct	–,390
R4400	T7021	T7019	prep	390,278
R4401	T7022	T7019	punct	),278
R4402	T7023	T7008	punct	.,contains
R4403	T7025	T7026	nsubjpass	This,made
R4404	T7027	T7026	auxpass	was,made
R4405	T7028	T7026	prep	by,made
R4406	T7029	T7028	pcomp	cutting,by
R4407	T7030	T7029	dobj	PAX6PST,cutting
R4408	T7031	T7029	prep	with,cutting
R4409	T7032	T7031	pobj	NdeI,with
R4410	T7033	T7032	cc	and,NdeI
R4411	T7034	T7032	conj	NotI,NdeI
R4412	T7035	T7036	aux	to,drop
R4413	T7036	T7029	advcl	drop,cutting
R4414	T7037	T7036	prt	out,drop
R4415	T7038	T7039	det	the,peptide
R4416	T7039	T7036	dobj	peptide,drop
R4417	T7040	T7041	npadvmod	C,terminal
R4418	T7041	T7039	amod	terminal,peptide
R4419	T7042	T7041	punct	-,terminal
R4420	T7043	T7029	punct	", ",cutting
R4421	T7044	T7029	cc	and,cutting
R4422	T7045	T7029	conj	inserting,cutting
R4423	T7046	T7047	det	a,linker
R4424	T7047	T7045	dobj	linker,inserting
R4425	T7048	T7047	amod	synthetic,linker
R4426	T7049	T7047	prep	between,linker
R4427	T7050	T7051	det	the,sites
R4428	T7051	T7049	pobj	sites,between
R4429	T7052	T7051	nummod	two,sites
R4430	T7053	T7051	compound	restriction,sites
R4431	T7054	T7026	punct	.,made
R4432	T7056	T7057	det	All,fragments
R4433	T7057	T7058	nsubjpass	fragments,sequenced
R4434	T7059	T7057	acl	generated,fragments
R4435	T7060	T7059	prep	by,generated
R4436	T7061	T7060	pobj	PCR,by
R4437	T7062	T7060	cc	or,by
R4438	T7063	T7060	conj	with,by
R4439	T7064	T7063	pobj	linkers,with
R4440	T7065	T7058	auxpass	were,sequenced
R4441	T7066	T7067	aux	to,check
R4442	T7067	T7058	advcl	check,sequenced
R4443	T7068	T7069	mark	that,introduced
R4444	T7069	T7067	ccomp	introduced,check
R4445	T7070	T7071	det	no,errors
R4446	T7071	T7069	nsubjpass	errors,introduced
R4447	T7072	T7069	aux	had,introduced
R4448	T7073	T7069	auxpass	been,introduced
R4449	T7074	T7058	punct	.,sequenced
R4450	T7076	T7077	det	A,construct
R4451	T7077	T7081	nsubjpass	construct,generated
R4452	T7078	T7079	compound	PAX6,domain
R4453	T7079	T7077	compound	domain,construct
R4454	T7080	T7079	compound	PST,domain
R4455	T7082	T7077	acl	containing,construct
R4456	T7083	T7084	det	the,mutation
R4457	T7084	T7082	dobj	mutation,containing
R4458	T7085	T7084	appos	1627A,mutation
R4459	T7086	T7087	punct	>,G
R4460	T7087	T7085	prep	G,1627A
R4461	T7088	T7084	punct	(,mutation
R4462	T7089	T7084	appos	Q422R,mutation
R4463	T7090	T7084	punct	),mutation
R4464	T7091	T7081	auxpass	was,generated
R4465	T7092	T7081	prep	in,generated
R4466	T7093	T7094	det	the,way
R4467	T7094	T7092	pobj	way,in
R4468	T7095	T7094	amod	same,way
R4469	T7096	T7094	prep	as,way
R4470	T7097	T7098	det	the,construct
R4471	T7098	T7096	pobj	construct,as
R4472	T7099	T7098	compound	PAX6PST,construct
R4473	T7100	T7092	punct	", ",in
R4474	T7101	T7092	cc	but,in
R4475	T7102	T7092	conj	using,in
R4476	T7103	T7104	det	the,primer
R4477	T7104	T7102	dobj	primer,using
R4478	T7105	T7104	amod	reverse,primer
R4479	T7106	T7104	compound	PCR,primer
R4480	T7107	T7108	nmod	ST006,AG
R4481	T7108	T7104	appos	AG,primer
R4482	T7109	T7108	nummod	5,AG
R4483	T7110	T7109	punct	',5
R4484	T7111	T7108	punct	-,AG
R4485	T7112	T7108	compound	TTT,AG
R4486	T7113	T7108	compound	TGC,AG
R4487	T7114	T7108	compound	GGC,AG
R4488	T7115	T7108	compound	CGC,AG
R4489	T7116	T7108	compound	TTT,AG
R4490	T7117	T7108	compound	TTA,AG
R4491	T7118	T7108	compound	CCG,AG
R4492	T7119	T7108	compound	TAA,AG
R4493	T7120	T7108	compound	TCT,AG
R4494	T7121	T7108	compound	TGG,AG
R4495	T7122	T7108	compound	CCA,AG
R4496	T7123	T7108	compound	GTA,AG
R4497	T7124	T7108	compound	TTG,AG
R4498	T7125	T7108	punct	-,AG
R4499	T7126	T7108	nummod	3,AG
R4500	T7127	T7108	punct	',AG
R4501	T7128	T7104	punct	", ",primer
R4502	T7129	T7130	dep	which,contains
R4503	T7130	T7104	relcl	contains,primer
R4504	T7131	T7132	det	the,substitution
R4505	T7132	T7130	dobj	substitution,contains
R4506	T7133	T7132	compound	mutant,substitution
R4507	T7134	T7132	compound	nucleotide,substitution
R4508	T7135	T7136	punct	(,underlined
R4509	T7136	T7104	parataxis	underlined,primer
R4510	T7137	T7136	punct	),underlined
R4511	T7138	T7081	punct	.,generated
R4512	T7140	T7141	compound	cDNA,sequences
R4513	T7141	T7142	nsubjpass	sequences,generated
R4514	T7143	T7141	acl	containing,sequences
R4515	T7144	T7145	det	the,mutations
R4516	T7145	T7143	dobj	mutations,containing
R4517	T7146	T7145	appos	1615del10,mutations
R4518	T7147	T7148	punct	[,28
R4519	T7148	T7146	parataxis	28,1615del10
R4520	T7149	T7148	punct	],28
R4521	T7150	T7146	cc	and,1615del10
R4522	T7151	T7146	conj	1629insT,1615del10
R4523	T7152	T7151	punct	(,1629insT
R4524	T7153	T7151	appos	X423L,1629insT
R4525	T7154	T7151	punct	),1629insT
R4526	T7155	T7156	punct	[,12
R4527	T7156	T7151	parataxis	12,1629insT
R4528	T7157	T7156	punct	],12
R4529	T7158	T7142	auxpass	were,generated
R4530	T7159	T7142	prep	by,generated
R4531	T7160	T7159	pobj	PCR,by
R4532	T7161	T7160	prep	from,PCR
R4533	T7162	T7163	amod	reverse,transcribed
R4534	T7163	T7164	amod	transcribed,RNA
R4535	T7164	T7161	pobj	RNA,from
R4536	T7165	T7166	punct	(,gift
R4537	T7166	T7164	parataxis	gift,RNA
R4538	T7167	T7166	det	a,gift
R4539	T7168	T7166	prep	from,gift
R4540	T7169	T7170	compound	Dr,Williamson
R4541	T7170	T7168	pobj	Williamson,from
R4542	T7171	T7170	compound	K,Williamson
R4543	T7172	T7170	cc	and,Williamson
R4544	T7173	T7174	compound	Prof,Heyningen
R4545	T7174	T7170	conj	Heyningen,Williamson
R4546	T7175	T7174	compound	V,Heyningen
R4547	T7176	T7174	compound	van,Heyningen
R4548	T7177	T7166	punct	),gift
R4549	T7178	T7142	punct	.,generated
R4550	T7180	T7181	nsubj	Primers,were
R4551	T7182	T7183	nmod	ST015,C
R4552	T7183	T7181	attr	C,were
R4553	T7184	T7185	punct	(,F
R4554	T7185	T7183	parataxis	F,C
R4555	T7186	T7185	punct	),F
R4556	T7187	T7183	nummod	5,C
R4557	T7188	T7187	punct	',5
R4558	T7189	T7183	punct	-,C
R4559	T7190	T7183	compound	CCC,C
R4560	T7191	T7183	compound	ACA,C
R4561	T7192	T7183	compound	TAT,C
R4562	T7193	T7183	compound	GCA,C
R4563	T7194	T7183	compound	GAC,C
R4564	T7195	T7183	compound	ACA,C
R4565	T7196	T7183	punct	-,C
R4566	T7197	T7183	nummod	3,C
R4567	T7198	T7183	punct	',C
R4568	T7199	T7183	cc	and,C
R4569	T7200	T7201	nmod	ST031,TC
R4570	T7201	T7183	conj	TC,C
R4571	T7202	T7203	punct	(,R
R4572	T7203	T7201	parataxis	R,TC
R4573	T7204	T7203	punct	),R
R4574	T7205	T7201	nummod	5,TC
R4575	T7206	T7205	punct	',5
R4576	T7207	T7201	punct	-,TC
R4577	T7208	T7201	compound	TTG,TC
R4578	T7209	T7201	compound	CGG,TC
R4579	T7210	T7201	compound	CCG,TC
R4580	T7211	T7201	compound	CAT,TC
R4581	T7212	T7201	compound	CCA,TC
R4582	T7213	T7201	compound	TCC,TC
R4583	T7214	T7201	compound	AGT,TC
R4584	T7215	T7201	compound	CTA,TC
R4585	T7216	T7201	compound	CAT,TC
R4586	T7217	T7201	compound	TGT,TC
R4587	T7218	T7201	punct	-,TC
R4588	T7219	T7201	nummod	3,TC
R4589	T7220	T7201	punct	',TC
R4590	T7221	T7181	punct	.,were
R4591	T7223	T7224	det	The,construct
R4592	T7224	T7226	nsubjpass	construct,cut
R4593	T7225	T7224	compound	PAX6PST,construct
R4594	T7227	T7226	auxpass	was,cut
R4595	T7228	T7226	prep	with,cut
R4596	T7229	T7228	pobj	NdeI,with
R4597	T7230	T7229	cc	and,NdeI
R4598	T7231	T7229	conj	NotI,NdeI
R4599	T7232	T7233	aux	to,release
R4600	T7233	T7226	advcl	release,cut
R4601	T7234	T7235	det	the,peptide
R4602	T7235	T7233	dobj	peptide,release
R4603	T7236	T7235	amod	normal,peptide
R4604	T7237	T7238	npadvmod	C,terminal
R4605	T7238	T7235	amod	terminal,peptide
R4606	T7239	T7238	punct	-,terminal
R4607	T7240	T7226	punct	", ",cut
R4608	T7241	T7226	cc	and,cut
R4609	T7242	T7243	det	the,sequence
R4610	T7243	T7245	nsubjpass	sequence,inserted
R4611	T7244	T7243	compound	mutant,sequence
R4612	T7245	T7226	conj	inserted,cut
R4613	T7246	T7245	auxpass	was,inserted
R4614	T7247	T7245	punct	.,inserted
R4615	T7368	T7369	nmod	Yeast,screens
R4616	T7370	T7371	nummod	two,hybrid
R4617	T7371	T7369	compound	hybrid,screens
R4618	T7372	T7371	punct	-,hybrid
R4619	T7373	T7369	compound	library,screens
R4620	T7375	T7376	det	A,library
R4621	T7376	T7380	nsubjpass	library,screened
R4622	T7377	T7378	compound	mouse,brain
R4623	T7378	T7376	compound	brain,library
R4624	T7379	T7376	compound	cDNA,library
R4625	T7381	T7382	punct	(,ProQuest
R4626	T7382	T7376	parataxis	ProQuest,library
R4627	T7383	T7382	punct	", ",ProQuest
R4628	T7384	T7382	npadvmod	Invitrogen,ProQuest
R4629	T7385	T7382	punct	),ProQuest
R4630	T7386	T7380	auxpass	was,screened
R4631	T7387	T7380	prep	with,screened
R4632	T7388	T7389	det	the,constructs
R4633	T7389	T7387	pobj	constructs,with
R4634	T7390	T7389	nmod	PAX6PST,constructs
R4635	T7391	T7390	cc	and,PAX6PST
R4636	T7392	T7390	conj	PAX6CTP,PAX6PST
R4637	T7393	T7389	compound	pDBLeu,constructs
R4638	T7394	T7380	punct	.,screened
R4639	T7396	T7397	det	The,library
R4640	T7397	T7398	nsubjpass	library,constructed
R4641	T7399	T7398	auxpass	was,constructed
R4642	T7400	T7398	prep	in,constructed
R4643	T7401	T7402	det	the,vector
R4644	T7402	T7400	pobj	vector,in
R4645	T7403	T7402	compound	pPC86,vector
R4646	T7404	T7402	punct	", ",vector
R4647	T7405	T7406	dep	which,produces
R4648	T7406	T7402	relcl	produces,vector
R4649	T7407	T7406	dobj	proteins,produces
R4650	T7408	T7407	acl	fused,proteins
R4651	T7409	T7408	prep	to,fused
R4652	T7410	T7411	det	the,domain
R4653	T7411	T7409	pobj	domain,to
R4654	T7412	T7411	compound	yeast,domain
R4655	T7413	T7411	compound	GAL4,domain
R4656	T7414	T7411	compound	activation,domain
R4657	T7415	T7398	punct	.,constructed
R4658	T7417	T7418	det	The,system
R4659	T7418	T7419	nsubj	system,uses
R4660	T7420	T7421	nummod	three,genes
R4661	T7421	T7419	dobj	genes,uses
R4662	T7422	T7423	npadvmod	GAL4,activated
R4663	T7423	T7421	amod	activated,genes
R4664	T7424	T7423	punct	-,activated
R4665	T7425	T7421	compound	reporter,genes
R4666	T7426	T7421	punct	", ",genes
R4667	T7427	T7421	appos	HIS3,genes
R4668	T7428	T7427	punct	", ",HIS3
R4669	T7429	T7427	conj	URA3,HIS3
R4670	T7430	T7429	cc	and,URA3
R4671	T7431	T7429	conj	lacZ,URA3
R4672	T7432	T7419	punct	", ",uses
R4673	T7433	T7434	aux	to,identify
R4674	T7434	T7419	advcl	identify,uses
R4675	T7435	T7436	amod	positive,interactions
R4676	T7436	T7434	dobj	interactions,identify
R4677	T7437	T7419	punct	.,uses
R4678	T7439	T7440	nsubjpass	Reporters,activated
R4679	T7441	T7440	auxpass	are,activated
R4680	T7442	T7443	advmod	when,interacts
R4681	T7443	T7440	advcl	interacts,activated
R4682	T7444	T7445	det	the,protein
R4683	T7445	T7443	nsubj	protein,interacts
R4684	T7446	T7445	compound	bait,protein
R4685	T7447	T7445	acl	fused,protein
R4686	T7448	T7447	prep	to,fused
R4687	T7449	T7450	det	the,domain
R4688	T7450	T7448	pobj	domain,to
R4689	T7451	T7450	compound	GAL4,domain
R4690	T7452	T7450	compound	DNA,domain
R4691	T7453	T7450	compound	binding,domain
R4692	T7454	T7450	punct	(,domain
R4693	T7455	T7450	appos	pDBLeu,domain
R4694	T7456	T7450	punct	),domain
R4695	T7457	T7443	prep	with,interacts
R4696	T7458	T7459	det	the,protein
R4697	T7459	T7457	pobj	protein,with
R4698	T7460	T7459	compound	prey,protein
R4699	T7461	T7459	acl	fused,protein
R4700	T7462	T7461	prep	to,fused
R4701	T7463	T7464	det	the,domain
R4702	T7464	T7462	pobj	domain,to
R4703	T7465	T7464	compound	GAL4,domain
R4704	T7466	T7464	compound	activation,domain
R4705	T7467	T7464	punct	(,domain
R4706	T7468	T7464	appos	pPC86,domain
R4707	T7469	T7464	punct	),domain
R4708	T7470	T7440	punct	", ",activated
R4709	T7471	T7472	advmod	thus,reconstituting
R4710	T7472	T7440	advcl	reconstituting,activated
R4711	T7473	T7474	compound	GAL4,function
R4712	T7474	T7472	dobj	function,reconstituting
R4713	T7475	T7440	punct	.,activated
R4714	T7477	T7478	compound	HIS3,activation
R4715	T7478	T7479	nsubj	activation,allows
R4716	T7480	T7479	dobj	growth,allows
R4717	T7481	T7479	prep	on,allows
R4718	T7482	T7481	pobj	plates,on
R4719	T7483	T7482	acl	lacking,plates
R4720	T7484	T7483	dobj	histidine,lacking
R4721	T7485	T7479	punct	.,allows
R4722	T7487	T7488	amod	Weak,activation
R4723	T7488	T7490	nsubj	activation,suppresses
R4724	T7489	T7488	compound	URA3,activation
R4725	T7491	T7490	dobj	growth,suppresses
R4726	T7492	T7490	prep	on,suppresses
R4727	T7493	T7492	pobj	plates,on
R4728	T7494	T7493	acl	containing,plates
R4729	T7495	T7496	advmod	5,orotic
R4730	T7496	T7500	amod	orotic,acid
R4731	T7497	T7496	punct	-,orotic
R4732	T7498	T7496	amod	fluro,orotic
R4733	T7499	T7496	punct	-,orotic
R4734	T7500	T7494	dobj	acid,containing
R4735	T7501	T7502	mark	while,permits
R4736	T7502	T7490	advcl	permits,suppresses
R4737	T7503	T7504	amod	strong,activation
R4738	T7504	T7502	nsubj	activation,permits
R4739	T7505	T7504	compound	URA3,activation
R4740	T7506	T7502	dobj	growth,permits
R4741	T7507	T7502	prep	on,permits
R4742	T7508	T7507	pobj	plates,on
R4743	T7509	T7508	acl	lacking,plates
R4744	T7510	T7509	dobj	uracil,lacking
R4745	T7511	T7490	punct	.,suppresses
R4746	T7513	T7514	compound	LacZ,activation
R4747	T7514	T7515	nsubj	activation,causes
R4748	T7516	T7517	compound	X,gal
R4749	T7517	T7519	nsubj	gal,turn
R4750	T7518	T7517	punct	-,gal
R4751	T7519	T7515	ccomp	turn,causes
R4752	T7520	T7519	aux	to,turn
R4753	T7521	T7519	oprd	blue,turn
R4754	T7522	T7521	prep	in,blue
R4755	T7523	T7524	det	a,assay
R4756	T7524	T7522	pobj	assay,in
R4757	T7525	T7526	compound	beta,galactosidase
R4758	T7526	T7524	compound	galactosidase,assay
R4759	T7527	T7526	punct	-,galactosidase
R4760	T7528	T7515	punct	.,causes
R4761	T7530	T7531	det	All,assays
R4762	T7531	T7532	nsubjpass	assays,carried
R4763	T7533	T7532	auxpass	were,carried
R4764	T7534	T7532	prt	out,carried
R4765	T7535	T7532	prep	in,carried
R4766	T7536	T7535	pobj	parallel,in
R4767	T7537	T7536	prep	with,parallel
R4768	T7538	T7539	det	the,strains
R4769	T7539	T7537	pobj	strains,with
R4770	T7540	T7539	nummod	five,strains
R4771	T7541	T7539	compound	ProQuest,strains
R4772	T7542	T7539	compound	control,strains
R4773	T7543	T7539	compound	yeast,strains
R4774	T7544	T7539	appos	A,strains
R4775	T7545	T7546	punct	-,E
R4776	T7546	T7544	prep	E,A
R4777	T7547	T7539	punct	", ",strains
R4778	T7548	T7549	dep	which,range
R4779	T7549	T7539	relcl	range,strains
R4780	T7550	T7549	prep	from,range
R4781	T7551	T7550	pobj	non-interactor,from
R4782	T7552	T7553	punct	(,A
R4783	T7553	T7551	parataxis	A,non-interactor
R4784	T7554	T7553	punct	),A
R4785	T7555	T7550	prep	to,from
R4786	T7556	T7557	amod	strong,interactor
R4787	T7557	T7555	pobj	interactor,to
R4788	T7558	T7559	punct	(,E
R4789	T7559	T7557	parataxis	E,interactor
R4790	T7560	T7559	punct	),E
R4791	T7561	T7532	punct	.,carried
R4792	T7563	T7564	det	All,procedures
R4793	T7564	T7565	nsubjpass	procedures,carried
R4794	T7566	T7565	auxpass	were,carried
R4795	T7567	T7565	prt	out,carried
R4796	T7568	T7565	prep	according,carried
R4797	T7569	T7568	prep	to,according
R4798	T7570	T7571	det	the,supplier
R4799	T7571	T7572	poss	supplier,protocols
R4800	T7572	T7569	pobj	protocols,to
R4801	T7573	T7571	case	's,supplier
R4802	T7574	T7565	punct	.,carried
R4803	T7576	T7577	advmod	Briefly,co-transformed
R4804	T7578	T7577	punct	", ",co-transformed
R4805	T7579	T7580	advmod	chemically,competent
R4806	T7580	T7581	amod	competent,cells
R4807	T7581	T7577	nsubjpass	cells,co-transformed
R4808	T7582	T7581	compound	MaV203,cells
R4809	T7583	T7581	compound	yeast,cells
R4810	T7584	T7577	auxpass	were,co-transformed
R4811	T7585	T7577	prep	with,co-transformed
R4812	T7586	T7587	det	the,library
R4813	T7587	T7585	pobj	library,with
R4814	T7588	T7587	compound	cDNA,library
R4815	T7589	T7587	cc	and,library
R4816	T7590	T7591	det	the,plasmid
R4817	T7591	T7587	conj	plasmid,library
R4818	T7592	T7591	compound	bait,plasmid
R4819	T7593	T7594	compound	pDBLeu,PAX6CTP
R4820	T7594	T7591	appos	PAX6CTP,plasmid
R4821	T7595	T7594	cc	or,PAX6CTP
R4822	T7596	T7597	compound	pDBLeu,PAX6PST
R4823	T7597	T7594	conj	PAX6PST,PAX6CTP
R4824	T7598	T7577	punct	.,co-transformed
R4825	T7600	T7601	nsubjpass	Transformants,plated
R4826	T7602	T7603	punct	(,107
R4827	T7603	T7600	parataxis	107,Transformants
R4828	T7604	T7603	nummod	5,107
R4829	T7605	T7603	punct	×,107
R4830	T7606	T7603	punct	),107
R4831	T7607	T7601	auxpass	were,plated
R4832	T7608	T7601	prep	on,plated
R4833	T7609	T7608	pobj	medium,on
R4834	T7610	T7609	acl	lacking,medium
R4835	T7611	T7610	dobj	histidine,lacking
R4836	T7612	T7613	aux	to,check
R4837	T7613	T7601	advcl	check,plated
R4838	T7614	T7613	prep	for,check
R4839	T7615	T7616	compound	HIS3,activation
R4840	T7616	T7614	pobj	activation,for
R4841	T7617	T7601	punct	.,plated
R4842	T7619	T7620	compound	HIS3,positives
R4843	T7620	T7621	nsubjpass	positives,assayed
R4844	T7622	T7621	auxpass	were,assayed
R4845	T7623	T7621	advmod	then,assayed
R4846	T7624	T7621	prep	for,assayed
R4847	T7625	T7626	det	all,reporters
R4848	T7626	T7624	pobj	reporters,for
R4849	T7627	T7626	nummod	3,reporters
R4850	T7628	T7621	punct	.,assayed
R4851	T7630	T7631	det	The,plasmid
R4852	T7631	T7634	nsubjpass	plasmid,isolated
R4853	T7632	T7631	compound	pPC86,plasmid
R4854	T7633	T7631	compound	prey,plasmid
R4855	T7635	T7634	auxpass	was,isolated
R4856	T7636	T7634	prep	from,isolated
R4857	T7637	T7638	det	all,positives
R4858	T7638	T7636	pobj	positives,from
R4859	T7639	T7640	compound	HIS3,LacZ
R4860	T7640	T7638	compound	LacZ,positives
R4861	T7641	T7640	punct	/,LacZ
R4862	T7642	T7640	compound	URA3,LacZ
R4863	T7643	T7640	punct	/,LacZ
R4864	T7644	T7634	cc	and,isolated
R4865	T7645	T7646	det	the,insert
R4866	T7646	T7648	nsubj	insert,sequenced
R4867	T7647	T7646	compound	cDNA,insert
R4868	T7648	T7634	conj	sequenced,isolated
R4869	T7649	T7648	punct	.,sequenced
R4870	T7651	T7652	compound	BLAST,searches
R4871	T7652	T7653	nsubjpass	searches,performed
R4872	T7654	T7653	auxpass	were,performed
R4873	T7655	T7656	aux	to,identify
R4874	T7656	T7653	advcl	identify,performed
R4875	T7657	T7658	det	the,insert
R4876	T7658	T7656	dobj	insert,identify
R4877	T7659	T7658	compound	cDNA,insert
R4878	T7660	T7661	punct	[,24
R4879	T7661	T7653	parataxis	24,performed
R4880	T7662	T7661	punct	],24
R4881	T7663	T7653	punct	.,performed
R4882	T7711	T7712	amod	Pairwise,interactions
R4883	T7714	T7715	amod	Specific,interactions
R4884	T7715	T7716	nsubjpass	interactions,tested
R4885	T7717	T7716	auxpass	were,tested
R4886	T7718	T7716	prep	by,tested
R4887	T7719	T7718	pcomp	transforming,by
R4888	T7720	T7721	amod	competent,cells
R4889	T7721	T7719	dobj	cells,transforming
R4890	T7722	T7721	compound	MaV203,cells
R4891	T7723	T7721	compound	yeast,cells
R4892	T7724	T7721	prep	with,cells
R4893	T7725	T7726	nummod	one,bait
R4894	T7726	T7727	compound	bait,construct
R4895	T7727	T7724	pobj	construct,with
R4896	T7728	T7727	punct	(,construct
R4897	T7729	T7727	prep	in,construct
R4898	T7730	T7729	pobj	pDBLeu,in
R4899	T7731	T7727	punct	),construct
R4900	T7732	T7727	cc	and,construct
R4901	T7733	T7734	nummod	one,prey
R4902	T7734	T7735	compound	prey,construct
R4903	T7735	T7727	conj	construct,construct
R4904	T7736	T7735	punct	(,construct
R4905	T7737	T7735	prep	in,construct
R4906	T7738	T7737	pobj	pPC86,in
R4907	T7739	T7719	punct	),transforming
R4908	T7740	T7719	cc	and,transforming
R4909	T7741	T7719	conj	testing,transforming
R4910	T7742	T7743	det	the,colonies
R4911	T7743	T7741	dobj	colonies,testing
R4912	T7744	T7743	amod	resulting,colonies
R4913	T7745	T7741	prep	for,testing
R4914	T7746	T7745	pobj	activation,for
R4915	T7747	T7746	prep	of,activation
R4916	T7748	T7749	det	the,reporters
R4917	T7749	T7747	pobj	reporters,of
R4918	T7750	T7749	nmod	HIS3,reporters
R4919	T7751	T7750	punct	", ",HIS3
R4920	T7752	T7750	conj	URA3,HIS3
R4921	T7753	T7752	cc	and,URA3
R4922	T7754	T7752	conj	LacZ,URA3
R4923	T7755	T7741	prep	as,testing
R4924	T7756	T7755	pcomp	before,as
R4925	T7757	T7716	punct	.,tested
R4926	T7759	T7760	aux	To,create
R4927	T7760	T7761	advcl	create,identified
R4928	T7762	T7763	det	a,clone
R4929	T7763	T7760	dobj	clone,create
R4930	T7764	T7765	amod	full,length
R4931	T7765	T7763	compound	length,clone
R4932	T7766	T7765	punct	-,length
R4933	T7767	T7763	compound	Homer3,clone
R4934	T7768	T7760	prep	for,create
R4935	T7769	T7770	amod	pairwise,tests
R4936	T7770	T7768	pobj	tests,for
R4937	T7771	T7761	punct	", ",identified
R4938	T7772	T7773	det	a,clone
R4939	T7773	T7761	nsubjpass	clone,identified
R4940	T7774	T7773	compound	cDNA,clone
R4941	T7775	T7776	punct	(,clone
R4942	T7776	T7773	parataxis	clone,clone
R4943	T7777	T7776	compound	IMAGE,clone
R4944	T7778	T7776	nummod	3602414,clone
R4945	T7779	T7776	punct	", ",clone
R4946	T7780	T7781	compound	accession,BE569374
R4947	T7781	T7776	appos	BE569374,clone
R4948	T7782	T7781	compound	number,BE569374
R4949	T7783	T7776	punct	),clone
R4950	T7784	T7773	acl	containing,clone
R4951	T7785	T7786	det	the,acids
R4952	T7786	T7784	dobj	acids,containing
R4953	T7787	T7786	amod	missing,acids
R4954	T7788	T7789	npadvmod	N,terminal
R4955	T7789	T7786	amod	terminal,acids
R4956	T7790	T7789	punct	-,terminal
R4957	T7791	T7786	nummod	70,acids
R4958	T7792	T7786	compound	amino,acids
R4959	T7793	T7761	auxpass	was,identified
R4960	T7794	T7761	prep	by,identified
R4961	T7795	T7796	det	a,search
R4962	T7796	T7794	pobj	search,by
R4963	T7797	T7796	compound	BLAST,search
R4964	T7798	T7796	prep	of,search
R4965	T7799	T7800	det	the,database
R4966	T7800	T7798	pobj	database,of
R4967	T7801	T7800	compound	EST,database
R4968	T7802	T7803	compound	nucleotide,sequence
R4969	T7803	T7800	compound	sequence,database
R4970	T7804	T7761	cc	and,identified
R4971	T7805	T7761	conj	obtained,identified
R4972	T7806	T7805	prep	from,obtained
R4973	T7807	T7808	det	the,Centre
R4974	T7808	T7806	pobj	Centre,from
R4975	T7809	T7810	compound	Rosalind,Franklin
R4976	T7810	T7808	compound	Franklin,Centre
R4977	T7811	T7808	prep	for,Centre
R4978	T7812	T7813	compound	Genomics,Research
R4979	T7813	T7811	pobj	Research,for
R4980	T7814	T7761	punct	.,identified
R4981	T7816	T7817	det	The,fragment
R4982	T7817	T7819	nsubj	fragment,amplified
R4983	T7818	T7817	amod	missing,fragment
R4984	T7820	T7819	aux	was,amplified
R4985	T7821	T7819	prep	from,amplified
R4986	T7822	T7823	det	the,clone
R4987	T7823	T7821	pobj	clone,from
R4988	T7824	T7823	compound	IMAGE,clone
R4989	T7825	T7819	prep	by,amplified
R4990	T7826	T7825	pobj	PCR,by
R4991	T7827	T7819	cc	and,amplified
R4992	T7828	T7819	conj	inserted,amplified
R4993	T7829	T7828	prep	into,inserted
R4994	T7830	T7831	det	the,plasmid
R4995	T7831	T7829	pobj	plasmid,into
R4996	T7832	T7833	compound	pPC86,Homer3
R4997	T7833	T7831	compound	Homer3,plasmid
R4998	T7834	T7833	punct	-,Homer3
R4999	T7835	T7836	aux	to,create
R5000	T7836	T7819	advcl	create,amplified
R5001	T7837	T7838	det	a,construct
R5002	T7838	T7836	dobj	construct,create
R5003	T7839	T7840	amod	full,length
R5004	T7840	T7838	compound	length,construct
R5005	T7841	T7840	punct	-,length
R5006	T7842	T7838	compound	expression,construct
R5007	T7843	T7819	punct	.,amplified
R5008	T7962	T7963	amod	Semi-quantitative,PCR
R5009	T7965	T7966	aux	To,check
R5010	T7966	T7967	advcl	check,performed
R5011	T7968	T7969	det	the,representation
R5012	T7969	T7966	dobj	representation,check
R5013	T7970	T7969	amod	relative,representation
R5014	T7971	T7969	prep	of,representation
R5015	T7972	T7971	pobj	clones,of
R5016	T7973	T7969	prep	in,representation
R5017	T7974	T7975	det	the,library
R5018	T7975	T7973	pobj	library,in
R5019	T7976	T7975	compound	cDNA,library
R5020	T7977	T7967	punct	", ",performed
R5021	T7978	T7979	amod	semi-quantitative,PCR
R5022	T7979	T7967	nsubjpass	PCR,performed
R5023	T7980	T7967	auxpass	was,performed
R5024	T7981	T7967	prep	on,performed
R5025	T7982	T7981	pobj	Pax6,on
R5026	T7983	T7982	punct	", ",Pax6
R5027	T7984	T7982	conj	Homer3,Pax6
R5028	T7985	T7984	punct	", ",Homer3
R5029	T7986	T7984	conj	Dncl1,Homer3
R5030	T7987	T7986	punct	", ",Dncl1
R5031	T7988	T7986	conj	Trim11,Dncl1
R5032	T7989	T7988	cc	and,Trim11
R5033	T7990	T7991	det	the,genes
R5034	T7991	T7988	conj	genes,Trim11
R5035	T7992	T7993	advmod	constitutively,expressed
R5036	T7993	T7991	amod	expressed,genes
R5037	T7994	T7991	appos	Gapdh,genes
R5038	T7995	T7994	cc	and,Gapdh
R5039	T7996	T7994	conj	Atp5a1,Gapdh
R5040	T7997	T7998	punct	[,30
R5041	T7998	T7967	parataxis	30,performed
R5042	T7999	T7998	nummod	29,30
R5043	T8000	T7998	punct	",",30
R5044	T8001	T7998	punct	],30
R5045	T8002	T7967	punct	.,performed
R5046	T8004	T8005	nsubjpass	Primers,designed
R5047	T8006	T8005	auxpass	were,designed
R5048	T8007	T8008	aux	to,cross
R5049	T8008	T8005	advcl	cross,designed
R5050	T8009	T8010	advmod	at,one
R5051	T8010	T8012	nummod	one,intron
R5052	T8011	T8010	advmod	least,one
R5053	T8012	T8008	dobj	intron,cross
R5054	T8013	T8005	punct	", ",designed
R5055	T8014	T8015	mark	so,amplified
R5056	T8015	T8005	advcl	amplified,designed
R5057	T8016	T8015	mark	that,amplified
R5058	T8017	T8018	advmod	only,clones
R5059	T8018	T8015	nsubjpass	clones,amplified
R5060	T8019	T8020	advmod	correctly,spliced
R5061	T8020	T8018	amod	spliced,clones
R5062	T8021	T8015	auxpass	were,amplified
R5063	T8022	T8005	punct	.,designed
R5064	T8024	T8025	compound	Primer,sequences
R5065	T8025	T8026	nsubj	sequences,were
R5066	T8027	T8026	punct	: ,were
R5067	T8028	T8026	attr	Pax6,were
R5068	T8029	T8028	punct	-,Pax6
R5069	T8030	T8031	compound	F,CTG
R5070	T8031	T8028	appos	CTG,Pax6
R5071	T8032	T8031	compound	CAG,CTG
R5072	T8033	T8031	compound	CCA,CTG
R5073	T8034	T8031	compound	AAA,CTG
R5074	T8035	T8031	compound	TAG,CTG
R5075	T8036	T8031	compound	ATC,CTG
R5076	T8037	T8031	compound	TAC,CTG
R5077	T8038	T8028	punct	;,Pax6
R5078	T8039	T8028	appos	Pax6,Pax6
R5079	T8040	T8039	punct	-,Pax6
R5080	T8041	T8042	compound	R,TT
R5081	T8042	T8039	appos	TT,Pax6
R5082	T8043	T8042	compound	CGA,TT
R5083	T8044	T8042	compound	TCA,TT
R5084	T8045	T8042	compound	CAT,TT
R5085	T8046	T8042	compound	GCT,TT
R5086	T8047	T8042	compound	CTC,TT
R5087	T8048	T8042	compound	TCC,TT
R5088	T8049	T8028	punct	;,Pax6
R5089	T8050	T8028	appos	Homer3,Pax6
R5090	T8051	T8050	punct	-,Homer3
R5091	T8052	T8053	compound	F,AGC
R5092	T8053	T8050	appos	AGC,Homer3
R5093	T8054	T8053	compound	CCC,AGC
R5094	T8055	T8053	compound	AGG,AGC
R5095	T8056	T8053	compound	TGG,AGC
R5096	T8057	T8053	compound	CTG,AGC
R5097	T8058	T8053	compound	TAG,AGC
R5098	T8059	T8028	punct	;,Pax6
R5099	T8060	T8028	appos	Homer3,Pax6
R5100	T8061	T8060	punct	-,Homer3
R5101	T8062	T8063	compound	R,G
R5102	T8063	T8060	appos	G,Homer3
R5103	T8064	T8063	compound	CTC,G
R5104	T8065	T8063	compound	TAC,G
R5105	T8066	T8063	compound	ACA,G
R5106	T8067	T8063	compound	GTG,G
R5107	T8068	T8063	compound	CAA,G
R5108	T8069	T8063	compound	AGC,G
R5109	T8070	T8063	compound	TCA,G
R5110	T8071	T8028	punct	;,Pax6
R5111	T8072	T8028	appos	Trim11,Pax6
R5112	T8073	T8072	punct	-,Trim11
R5113	T8074	T8075	compound	F,CTG
R5114	T8075	T8072	appos	CTG,Trim11
R5115	T8076	T8075	compound	GTG,CTG
R5116	T8077	T8075	compound	CAG,CTG
R5117	T8078	T8075	compound	GAT,CTG
R5118	T8079	T8075	compound	GTG,CTG
R5119	T8080	T8075	compound	AAG,CTG
R5120	T8081	T8028	punct	;,Pax6
R5121	T8082	T8028	appos	Trim11,Pax6
R5122	T8083	T8082	punct	-,Trim11
R5123	T8084	T8085	compound	R,GG
R5124	T8085	T8082	appos	GG,Trim11
R5125	T8086	T8085	compound	GCC,GG
R5126	T8087	T8085	compound	TGC,GG
R5127	T8088	T8085	compound	AGA,GG
R5128	T8089	T8085	compound	TAG,GG
R5129	T8090	T8085	compound	TCA,GG
R5130	T8091	T8085	compound	TAG,GG
R5131	T8092	T8028	punct	;,Pax6
R5132	T8093	T8028	appos	Dncl1,Pax6
R5133	T8094	T8093	punct	-,Dncl1
R5134	T8095	T8096	compound	F,G
R5135	T8096	T8093	appos	G,Dncl1
R5136	T8097	T8096	compound	CAA,G
R5137	T8098	T8096	compound	AAA,G
R5138	T8099	T8096	compound	TGC,G
R5139	T8100	T8096	compound	AGA,G
R5140	T8101	T8096	compound	CAT,G
R5141	T8102	T8096	compound	GTC,G
R5142	T8103	T8028	punct	;,Pax6
R5143	T8104	T8028	appos	Dncl1,Pax6
R5144	T8105	T8104	punct	-,Dncl1
R5145	T8106	T8107	compound	R,GGG
R5146	T8107	T8104	appos	GGG,Dncl1
R5147	T8108	T8107	compound	CTA,GGG
R5148	T8109	T8107	compound	AGG,GGG
R5149	T8110	T8107	compound	GAG,GGG
R5150	T8111	T8107	compound	AAA,GGG
R5151	T8112	T8107	compound	AAA,GGG
R5152	T8113	T8107	compound	ATG,GGG
R5153	T8114	T8028	punct	;,Pax6
R5154	T8115	T8116	compound	Gapdh,F
R5155	T8116	T8028	appos	F,Pax6
R5156	T8117	T8116	punct	-,F
R5157	T8118	T8116	punct	: ,F
R5158	T8119	T8120	compound	CAT,GC
R5159	T8120	T8116	appos	GC,F
R5160	T8121	T8120	compound	CAC,GC
R5161	T8122	T8120	compound	CAT,GC
R5162	T8123	T8120	compound	CTT,GC
R5163	T8124	T8120	compound	CCA,GC
R5164	T8125	T8120	compound	GGA,GC
R5165	T8126	T8028	punct	;,Pax6
R5166	T8127	T8128	compound	Gapdh,R
R5167	T8128	T8028	appos	R,Pax6
R5168	T8129	T8128	punct	-,R
R5169	T8130	T8128	punct	: ,R
R5170	T8131	T8132	compound	ATG,TT
R5171	T8132	T8128	appos	TT,R
R5172	T8133	T8132	compound	ACC,TT
R5173	T8134	T8132	compound	TTG,TT
R5174	T8135	T8132	compound	CCC,TT
R5175	T8136	T8132	compound	ACA,TT
R5176	T8137	T8132	compound	GCC,TT
R5177	T8138	T8028	punct	;,Pax6
R5178	T8139	T8140	compound	Atp5a1,F
R5179	T8140	T8028	appos	F,Pax6
R5180	T8141	T8140	punct	-,F
R5181	T8142	T8140	punct	: ,F
R5182	T8143	T8144	compound	CAC,CA
R5183	T8144	T8140	appos	CA,F
R5184	T8145	T8144	compound	ACG,CA
R5185	T8146	T8144	compound	TGA,CA
R5186	T8147	T8144	compound	GAT,CA
R5187	T8148	T8144	compound	GTC,CA
R5188	T8149	T8144	compound	CTC,CA
R5189	T8150	T8028	punct	;,Pax6
R5190	T8151	T8152	compound	Atp5a1,R
R5191	T8152	T8028	appos	R,Pax6
R5192	T8153	T8152	punct	-,R
R5193	T8154	T8152	punct	: ,R
R5194	T8155	T8156	compound	CAC,A
R5195	T8156	T8152	appos	A,R
R5196	T8157	T8156	compound	AGA,A
R5197	T8158	T8156	compound	GAT,A
R5198	T8159	T8156	compound	TCG,A
R5199	T8160	T8156	compound	GGG,A
R5200	T8161	T8156	compound	ATA,A
R5201	T8162	T8026	punct	.,were
R5202	T8164	T8165	nummod	10,ng
R5203	T8165	T8166	compound	ng,cDNA
R5204	T8166	T8168	nsubjpass	cDNA,amplified
R5205	T8167	T8166	compound	library,cDNA
R5206	T8169	T8168	auxpass	were,amplified
R5207	T8170	T8168	prep	in,amplified
R5208	T8171	T8172	det	a,reaction
R5209	T8172	T8170	pobj	reaction,in
R5210	T8173	T8172	acl	containing,reaction
R5211	T8174	T8175	nummod	1,buffer
R5212	T8175	T8173	dobj	buffer,containing
R5213	T8176	T8174	punct	x,1
R5214	T8177	T8175	compound	AmpliTaq,buffer
R5215	T8178	T8175	compound	polymerase,buffer
R5216	T8179	T8180	punct	(,Elmer
R5217	T8180	T8175	parataxis	Elmer,buffer
R5218	T8181	T8180	compound	Perkin,Elmer
R5219	T8182	T8180	punct	),Elmer
R5220	T8183	T8175	punct	", ",buffer
R5221	T8184	T8185	nummod	1.5,mM
R5222	T8185	T8186	compound	mM,MgCl2
R5223	T8186	T8175	conj	MgCl2,buffer
R5224	T8187	T8186	punct	", ",MgCl2
R5225	T8188	T8189	nmod	200μM,primer
R5226	T8189	T8186	conj	primer,MgCl2
R5227	T8190	T8189	det	each,primer
R5228	T8191	T8189	cc	and,primer
R5229	T8192	T8193	nummod	2.5,units
R5230	T8193	T8189	conj	units,primer
R5231	T8194	T8193	prep	of,units
R5232	T8195	T8196	compound	AmpliTaq,polymerase
R5233	T8196	T8194	pobj	polymerase,of
R5234	T8197	T8198	punct	(,Elmer
R5235	T8198	T8196	parataxis	Elmer,polymerase
R5236	T8199	T8198	compound	Perkin,Elmer
R5237	T8200	T8198	punct	),Elmer
R5238	T8201	T8168	punct	.,amplified
R5239	T8203	T8204	compound	PCR,conditions
R5240	T8204	T8205	nsubj	conditions,were
R5241	T8206	T8205	punct	(,were
R5242	T8207	T8208	nummod	95,°C
R5243	T8208	T8205	attr	°C,were
R5244	T8209	T8208	prep	for,°C
R5245	T8210	T8211	nummod	30,sec
R5246	T8211	T8209	pobj	sec,for
R5247	T8212	T8208	punct	),°C
R5248	T8213	T8208	punct	×,°C
R5249	T8214	T8208	appos	1,°C
R5250	T8215	T8208	punct	", ",°C
R5251	T8216	T8217	punct	(,°C
R5252	T8217	T8208	conj	°C,°C
R5253	T8218	T8217	nummod	94,°C
R5254	T8219	T8217	prep	for,°C
R5255	T8220	T8221	nummod	30,sec
R5256	T8221	T8219	pobj	sec,for
R5257	T8222	T8217	punct	", ",°C
R5258	T8223	T8224	nummod	55,°C
R5259	T8224	T8217	appos	°C,°C
R5260	T8225	T8224	prep	for,°C
R5261	T8226	T8227	nummod	30,sec
R5262	T8227	T8225	pobj	sec,for
R5263	T8228	T8217	punct	", ",°C
R5264	T8229	T8230	nummod	72,°C
R5265	T8230	T8217	appos	°C,°C
R5266	T8231	T8230	prep	for,°C
R5267	T8232	T8233	nummod	30,sec
R5268	T8233	T8231	pobj	sec,for
R5269	T8234	T8217	punct	),°C
R5270	T8235	T8217	punct	×,°C
R5271	T8236	T8217	appos	32,°C
R5272	T8237	T8217	cc	and,°C
R5273	T8238	T8239	punct	(,°C
R5274	T8239	T8217	conj	°C,°C
R5275	T8240	T8239	nummod	72,°C
R5276	T8241	T8239	prep	for,°C
R5277	T8242	T8243	nummod	2,min
R5278	T8243	T8241	pobj	min,for
R5279	T8244	T8239	punct	),°C
R5280	T8245	T8239	punct	×,°C
R5281	T8246	T8239	appos	1,°C
R5282	T8247	T8205	punct	.,were
R5283	T8249	T8250	nsubjpass	Products,resolved
R5284	T8251	T8250	auxpass	were,resolved
R5285	T8252	T8250	prep	on,resolved
R5286	T8253	T8254	det	a,gel
R5287	T8254	T8252	pobj	gel,on
R5288	T8255	T8256	nummod	2.5,%
R5289	T8256	T8254	compound	%,gel
R5290	T8257	T8254	compound	agarose,gel
R5291	T8258	T8250	prep	with,resolved
R5292	T8259	T8260	compound	ΦX174,HaeIII
R5293	T8260	T8262	compound	HaeIII,markers
R5294	T8261	T8260	punct	/,HaeIII
R5295	T8262	T8258	pobj	markers,with
R5296	T8263	T8262	compound	size,markers
R5297	T8264	T8265	punct	(,Promega
R5298	T8265	T8262	parataxis	Promega,markers
R5299	T8266	T8265	punct	),Promega
R5300	T8267	T8250	punct	.,resolved

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id	Subject	Object	Predicate	Lexical cue
T7961	5739-5746	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	markers
T7960	5721-5726	NCBITaxon:1910954	denotes	ΦX174
T7959	5704-5711	CHEBI:2511	denotes	agarose
T7958	5518-5528	GO_EXT:polymerase	denotes	polymerase
T7957	5485-5491	SO_EXT:0000112	denotes	primer
T7956	5467-5472	CHEBI:6636	denotes	MgCl2
T7955	5437-5443	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T7954	5426-5436	GO_EXT:polymerase	denotes	polymerase
T7953	5370-5374	SO_EXT:cDNA	denotes	cDNA
T7952	5326-5327	SO:0001031	denotes	R
T7951	5319-5325	PR_EXT:000004453	denotes	Atp5a1
T7950	5288-5289	SO:0001030	denotes	F
T7949	5281-5287	PR_EXT:000004453	denotes	Atp5a1
T7948	5250-5251	SO:0001031	denotes	R
T7947	5244-5249	GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase	denotes	Gapdh
T7946	5213-5214	SO:0001030	denotes	F
T7945	5207-5212	GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase	denotes	Gapdh
T7944	5176-5177	SO:0001031	denotes	R
T7943	5170-5175	PR_EXT:000002325	denotes	Dncl1
T7942	5141-5142	SO:0001030	denotes	F
T7941	5135-5140	PR_EXT:000002325	denotes	Dncl1
T7940	5105-5106	SO:0001031	denotes	R
T7939	5098-5104	PR_EXT:000016642	denotes	Trim11
T7938	5071-5072	SO:0001030	denotes	F
T7937	5064-5070	PR_EXT:000016642	denotes	Trim11
T7936	5031-5032	SO:0001031	denotes	R
T7935	5024-5030	PR_EXT:000008680	denotes	Homer3
T7934	4997-4998	SO:0001030	denotes	F
T7933	4990-4996	PR_EXT:000008680	denotes	Homer3
T7932	4960-4961	SO:0001031	denotes	R
T7931	4955-4959	PR_EXT:000012318	denotes	Pax6
T7930	4924-4925	SO:0001030	denotes	F
T7929	4919-4923	PR_EXT:000012318	denotes	Pax6
T7928	4903-4912	SO_EXT:biological_sequence	denotes	sequences
T7927	4896-4902	SO_EXT:0000112	denotes	Primer
T7926	4873-4879	SO_EXT:sequence_cloned_entity	denotes	clones
T7925	4865-4872	GO:0008380	denotes	spliced
T7924	4834-4840	SO_EXT:0000188	denotes	intron
T7923	4790-4797	SO_EXT:0000112	denotes	Primers
T7922	4774-4780	PR_EXT:000004453	denotes	Atp5a1
T7921	4764-4769	GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase	denotes	Gapdh
T7920	4758-4763	SO_EXT:0000704	denotes	genes
T7918	4718-4724	PR_EXT:000016642	denotes	Trim11
T7917	4711-4716	PR_EXT:000002325	denotes	Dncl1
T7916	4703-4709	PR_EXT:000008680	denotes	Homer3
T7710	4556-4565	SO_EXT:engineered_biological_sequence	denotes	construct
T7709	4545-4555	GO:0010467	denotes	expression
T7708	4513-4520	SO_EXT:0000155	denotes	plasmid
T7707	4506-4512	PR_EXT:000008680	denotes	Homer3
T7706	4482-4490	SO_EXT:sequence_insertion_process	denotes	inserted
T7705	4465-4470	SO_EXT:sequence_cloned_entity	denotes	clone
T7704	4327-4335	SO_EXT:biological_sequence	denotes	sequence
T7703	4316-4326	CHEBI_SO_EXT:nucleotide	denotes	nucleotide
T7702	4312-4315	SO_EXT:0000345	denotes	EST
T7701	4260-4271	CHEBI_SO_EXT:amino_acid	denotes	amino acids
T7700	4246-4256	CHEBI_SO_EXT:N_terminus_or_N_terminal_region	denotes	N-terminal
T7699	4181-4186	SO_EXT:sequence_cloned_entity	denotes	clone
T7698	4163-4173	SO_EXT:0000317	denotes	cDNA clone
T7697	4135-4140	SO_EXT:sequence_cloned_entity	denotes	clone
T7696	4128-4134	PR_EXT:000008680	denotes	Homer3
T7695	4078-4082	PR_EXT:000033987	denotes	LacZ
T7694	4069-4073	PR_EXT:P03962	denotes	URA3
T7693	4063-4067	PR_EXT:P06633	denotes	HIS3
T7692	3985-3994	SO_EXT:engineered_biological_sequence	denotes	construct
T7691	3950-3959	SO_EXT:engineered_biological_sequence	denotes	construct
T7690	3930-3935	CL_GO_EXT:cell	denotes	cells
T7689	3924-3929	NCBITaxon_EXT:yeast	denotes	yeast
T7688	3894-3906	GO:0009294	denotes	transforming
T7367	3821-3827	SO_EXT:0000667	denotes	insert
T7366	3816-3820	SO_EXT:cDNA	denotes	cDNA
T7365	3752-3758	SO_EXT:0000667	denotes	insert
T7364	3747-3751	SO_EXT:cDNA	denotes	cDNA
T7363	3724-3728	PR_EXT:000033987	denotes	LacZ
T7362	3719-3723	PR_EXT:P03962	denotes	URA3
T7361	3714-3718	PR_EXT:P06633	denotes	HIS3
T7360	3684-3691	SO_EXT:0000155	denotes	plasmid
T7359	3615-3619	PR_EXT:P06633	denotes	HIS3
T7358	3598-3602	PR_EXT:P06633	denotes	HIS3
T7357	3575-3584	CHEBI_SO_EXT:histidine	denotes	histidine
T7356	3521-3534	GO_EXT:transfectant_or_tranformant	denotes	Transformants
T7355	3479-3486	SO_EXT:0000155	denotes	plasmid
T7354	3453-3457	SO_EXT:cDNA	denotes	cDNA
T7353	3432-3443	GO:0009294	denotes	transformed
T7352	3418-3423	CL_GO_EXT:cell	denotes	cells
T7351	3412-3417	NCBITaxon_EXT:yeast	denotes	yeast
T7350	3384-3394	CHEBI_EXT:chemical_substance_or_polyatomic_entity	denotes	chemically
T7349	3223-3228	NCBITaxon_EXT:yeast	denotes	yeast
T7348	3126-3144	GO_EXT:0004565	denotes	beta-galactosidase
T7347	3102-3107	CHEBI:75055	denotes	X-gal
T7346	3079-3083	PR_EXT:000033987	denotes	LacZ
T7345	3071-3077	CHEBI_EXT:uracil_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	uracil
T7344	3046-3052	GO_EXT:biological_growth_entity_or_process	denotes	growth
T7343	3022-3026	PR_EXT:P03962	denotes	URA3
T7342	2989-3008	CHEBI:74498	denotes	5-fluro-orotic acid
T7341	2961-2967	GO_EXT:biological_growth_entity_or_process	denotes	growth
T7340	2934-2938	PR_EXT:P03962	denotes	URA3
T7339	2918-2927	CHEBI_SO_EXT:histidine	denotes	histidine
T7338	2893-2899	GO_EXT:biological_growth_entity_or_process	denotes	growth
T7337	2870-2874	PR_EXT:P06633	denotes	HIS3
T7336	2855-2859	PR_EXT:P04386	denotes	GAL4
T7335	2819-2825	SO_EXT:0000417	denotes	domain
T7334	2803-2807	PR_EXT:P04386	denotes	GAL4
T7333	2790-2795	SO_EXT:sequence_fusion_process	denotes	fused
T7332	2782-2789	CHEBI_PR_EXT:protein	denotes	protein
T7331	2742-2748	SO_EXT:0000417	denotes	domain
T7330	2734-2741	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T7329	2730-2733	CHEBI_SO_EXT:DNA	denotes	DNA
T7328	2725-2729	PR_EXT:P04386	denotes	GAL4
T7327	2712-2717	SO_EXT:sequence_fusion_process	denotes	fused
T7326	2704-2711	CHEBI_PR_EXT:protein	denotes	protein
T7325	2625-2629	PR_EXT:000033987	denotes	lacZ
T7324	2616-2620	PR_EXT:P03962	denotes	URA3
T7323	2610-2614	PR_EXT:P06633	denotes	HIS3
T7322	2603-2608	SO_EXT:0000704	denotes	genes
T7321	2579-2583	PR_EXT:P04386	denotes	GAL4
T7320	2549-2555	SO_EXT:0000417	denotes	domain
T7319	2533-2537	PR_EXT:P04386	denotes	GAL4
T7318	2527-2532	NCBITaxon_EXT:yeast	denotes	yeast
T7317	2514-2519	SO_EXT:sequence_fusion_process	denotes	fused
T7316	2505-2513	CHEBI_PR_EXT:protein	denotes	proteins
T7315	2482-2488	SO_EXT:0000440	denotes	vector
T7314	2429-2439	SO_EXT:engineered_biological_sequence	denotes	constructs
T7313	2344-2348	SO_EXT:cDNA	denotes	cDNA
T7312	2338-2343	UBERON:0000955	denotes	brain
T7311	2332-2337	NCBITaxon:10088	denotes	mouse
T7310	2297-2302	NCBITaxon_EXT:yeast	denotes	Yeast
T6594	385-394	SO:0000993	denotes	consensus
T6593	286-290	PR_EXT:000012318	denotes	PAX6
T6592	280-285	NCBITaxon:9606	denotes	human
T6591	266-276	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminus
T6590	248-258	SO:0000857	denotes	homologous
T6589	221-230	SO_EXT:biological_sequence	denotes	sequences
T6588	213-220	CHEBI_PR_EXT:protein	denotes	Protein
T6587	35-43	SO_EXT:biological_sequence	denotes	Sequence
T6807	2286-2294	SO_EXT:sequence_insertion_process	denotes	inserted
T6806	2273-2281	SO_EXT:biological_sequence	denotes	sequence
T6805	2266-2272	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6804	2249-2256	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6803	2238-2248	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminal
T6802	2219-2226	GO_EXT:biological_release	denotes	release
T6801	2193-2196	MOP:0000780	denotes	cut
T6800	2179-2188	SO_EXT:engineered_biological_sequence	denotes	construct
T6799	2114-2115	SO:0001031	denotes	R
T6798	2068-2069	SO:0001030	denotes	F
T6797	2048-2055	SO_EXT:0000112	denotes	Primers
T6796	1988-1991	CHEBI_SO_EXT:RNA	denotes	RNA
T6795	1968-1987	GO:0001171	denotes	reverse transcribed
T6794	1890-1899	SO_EXT:sequence_alteration_entity_or_process	denotes	mutations
T6793	1865-1874	SO_EXT:biological_sequence	denotes	sequences
T6792	1860-1864	SO_EXT:cDNA	denotes	cDNA
T6791	1833-1845	SO_EXT:sequence_substitution_entity_or_process	denotes	substitution
T6790	1822-1832	CHEBI_SO_EXT:nucleotide	denotes	nucleotide
T6789	1815-1821	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6788	1709-1716,1721-1727	SO_EXT:0000132	denotes	reverse primer
T6786	1684-1693	SO_EXT:engineered_biological_sequence	denotes	construct
T6785	1629-1630	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T6784	1614-1622	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T6783	1589-1598	SO_EXT:engineered_biological_sequence	denotes	construct
T6782	1582-1588	SO_EXT:0000417	denotes	domain
T6781	1573-1577	PR_EXT:000012318	denotes	PAX6
T6780	1445-1462	SO_EXT:0000061	denotes	restriction sites
T6779	1400-1409	SO_EXT:sequence_insertion_process	denotes	inserting
T6778	1387-1394	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6777	1376-1386	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminal
T6776	1325-1332	MOP:0000780	denotes	cutting
T6775	1289-1296	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6774	1278-1288	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminal
T6773	1261-1267	SO_EXT:0000417	denotes	domain
T6772	1229-1230	SO:0001031	denotes	R
T6771	1166-1167	SO:0001030	denotes	F
T6770	1146-1153	SO_EXT:0000112	denotes	Primers
T6769	1121-1128	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T6768	1110-1120	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminal
T6767	1017-1018	SO:0001031	denotes	R
T6766	949-956	SO:0001030	denotes	forward
T6765	929-936	SO_EXT:0000112	denotes	Primers
T6764	919-926	CHEBI_PR_EXT:protein	denotes	protein
T6763	914-918	PR_EXT:000012318	denotes	PAX6
T6762	875-886	CHEBI_SO_EXT:amino_acid	denotes	amino acids
T6761	867-873	SO_EXT:0000417	denotes	domain
T6760	828-834	SO_EXT:0000417	denotes	domain
T6759	820-827	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T6758	816-819	CHEBI_SO_EXT:DNA	denotes	DNA
T6757	811-815	PR_EXT:P04386	denotes	GAL4
T6756	805-810	NCBITaxon_EXT:yeast	denotes	yeast
T6755	792-797	SO_EXT:sequence_fusion_process	denotes	fused
T6754	784-791	CHEBI_PR_EXT:protein	denotes	protein
T6753	717-723	SO_EXT:0000440	denotes	vector
T6752	706-716	GO:0010467	denotes	expression
T6751	681-691	SO_EXT:engineered_biological_sequence	denotes	constructs
T6750	676-680	SO_EXT:cDNA	denotes	cDNA
T6749	671-675	PR_EXT:000012318	denotes	PAX6
T6748	576-583	SO_EXT:sequence_variant_or_variation	denotes	Variant
T6747	568-575	SO_EXT:0001023	denotes	Allelic
T6746	563-567	PR_EXT:000012318	denotes	PAX6
T6745	557-562	NCBITaxon:9606	denotes	Human
T6744	528-537	SO_EXT:biological_sequence	denotes	sequences
T6743	510-517	CHEBI_PR_EXT:protein	denotes	protein
T6742	501-505	SO_EXT:cDNA	denotes	cDNA
T6741	496-500	PR_EXT:000012318	denotes	PAX6
T6740	490-495	NCBITaxon:9606	denotes	human
T6739	453-463	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T6738	444-448	SO_EXT:cDNA	denotes	cDNA
T6737	429-439	SO_EXT:engineered_biological_sequence	denotes	constructs
T6736	412-417	NCBITaxon_EXT:yeast	denotes	Yeast
T7919	4748-4757	GO:0010467	denotes	expressed
T7915	4697-4701	PR_EXT:000012318	denotes	Pax6
T7914	4644-4648	SO_EXT:cDNA	denotes	cDNA
T7913	4630-4636	SO_EXT:sequence_cloned_entity	denotes	clones

craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id	Subject	Object	Predicate	Lexical cue
T7912	5721-5726	NCBITaxon:1910954	denotes	ΦX174
T7911	5704-5711	CHEBI:2511	denotes	agarose
T7910	5485-5491	SO:0000112	denotes	primer
T7909	5467-5472	CHEBI:6636	denotes	MgCl2
T7908	5326-5327	SO:0001031	denotes	R
T7907	5319-5325	PR:000004453	denotes	Atp5a1
T7906	5288-5289	SO:0001030	denotes	F
T7905	5281-5287	PR:000004453	denotes	Atp5a1
T7687	4545-4555	GO:0010467	denotes	expression
T7686	4513-4520	SO:0000155	denotes	plasmid
T7685	4506-4512	PR:000008680	denotes	Homer3
T7684	4312-4315	SO:0000345	denotes	EST
T7683	4163-4173	SO:0000317	denotes	cDNA clone
T7682	4128-4134	PR:000008680	denotes	Homer3
T7681	4078-4082	PR:000033987	denotes	LacZ
T7680	4069-4073	PR:P03962	denotes	URA3
T7679	4063-4067	PR:P06633	denotes	HIS3
T7678	3894-3906	GO:0009294	denotes	transforming
T7904	5250-5251	SO:0001031	denotes	R
T7903	5213-5214	SO:0001030	denotes	F
T7902	5176-5177	SO:0001031	denotes	R
T7901	5170-5175	PR:000002325	denotes	Dncl1
T7900	5141-5142	SO:0001030	denotes	F
T7899	5135-5140	PR:000002325	denotes	Dncl1
T7898	5105-5106	SO:0001031	denotes	R
T7897	5098-5104	PR:000016642	denotes	Trim11
T7896	5071-5072	SO:0001030	denotes	F
T7895	5064-5070	PR:000016642	denotes	Trim11
T7894	5031-5032	SO:0001031	denotes	R
T7893	5024-5030	PR:000008680	denotes	Homer3
T7892	4997-4998	SO:0001030	denotes	F
T7891	4990-4996	PR:000008680	denotes	Homer3
T7890	4960-4961	SO:0001031	denotes	R
T7889	4955-4959	PR:000012318	denotes	Pax6
T7888	4924-4925	SO:0001030	denotes	F
T7887	4919-4923	PR:000012318	denotes	Pax6
T7886	4896-4902	SO:0000112	denotes	Primer
T7885	4865-4872	GO:0008380	denotes	spliced
T7884	4834-4840	SO:0000188	denotes	intron
T7883	4790-4797	SO:0000112	denotes	Primers
T7882	4774-4780	PR:000004453	denotes	Atp5a1
T7881	4758-4763	SO:0000704	denotes	genes
T7880	4748-4757	GO:0010467	denotes	expressed
T7879	4718-4724	PR:000016642	denotes	Trim11
T7878	4711-4716	PR:000002325	denotes	Dncl1
T7877	4703-4709	PR:000008680	denotes	Homer3
T7876	4697-4701	PR:000012318	denotes	Pax6
T7309	3821-3827	SO:0000667	denotes	insert
T7308	3752-3758	SO:0000667	denotes	insert
T7307	3724-3728	PR:000033987	denotes	LacZ
T7306	3719-3723	PR:P03962	denotes	URA3
T7305	3714-3718	PR:P06633	denotes	HIS3
T7304	3684-3691	SO:0000155	denotes	plasmid
T7303	3615-3619	PR:P06633	denotes	HIS3
T7302	3598-3602	PR:P06633	denotes	HIS3
T7301	3479-3486	SO:0000155	denotes	plasmid
T7300	3432-3443	GO:0009294	denotes	transformed
T7299	3102-3107	CHEBI:75055	denotes	X-gal
T7298	3079-3083	PR:000033987	denotes	LacZ
T7297	3022-3026	PR:P03962	denotes	URA3
T7296	2989-3008	CHEBI:74498	denotes	5-fluro-orotic acid
T7295	2934-2938	PR:P03962	denotes	URA3
T7294	2870-2874	PR:P06633	denotes	HIS3
T7293	2855-2859	PR:P04386	denotes	GAL4
T7292	2819-2825	SO:0000417	denotes	domain
T7291	2803-2807	PR:P04386	denotes	GAL4
T7290	2742-2748	SO:0000417	denotes	domain
T7289	2725-2729	PR:P04386	denotes	GAL4
T7288	2625-2629	PR:000033987	denotes	lacZ
T7287	2616-2620	PR:P03962	denotes	URA3
T7286	2610-2614	PR:P06633	denotes	HIS3
T7285	2603-2608	SO:0000704	denotes	genes
T7284	2579-2583	PR:P04386	denotes	GAL4
T7283	2549-2555	SO:0000417	denotes	domain
T7282	2533-2537	PR:P04386	denotes	GAL4
T7281	2482-2488	SO:0000440	denotes	vector
T7280	2338-2343	UBERON:0000955	denotes	brain
T7279	2332-2337	NCBITaxon:10088	denotes	mouse
T6719	949-956	SO:0001030	denotes	forward
T6718	929-936	SO:0000112	denotes	Primers
T6717	914-918	PR:000012318	denotes	PAX6
T6716	867-873	SO:0000417	denotes	domain
T6715	828-834	SO:0000417	denotes	domain
T6714	811-815	PR:P04386	denotes	GAL4
T6713	717-723	SO:0000440	denotes	vector
T6712	706-716	GO:0010467	denotes	expression
T6711	671-675	PR:000012318	denotes	PAX6
T6710	568-575	SO:0001023	denotes	Allelic
T6709	563-567	PR:000012318	denotes	PAX6
T6708	557-562	NCBITaxon:9606	denotes	Human
T6707	496-500	PR:000012318	denotes	PAX6
T6706	490-495	NCBITaxon:9606	denotes	human
T6586	385-394	SO:0000993	denotes	consensus
T6585	286-290	PR:000012318	denotes	PAX6
T6584	280-285	NCBITaxon:9606	denotes	human
T6583	248-258	SO:0000857	denotes	homologous
T6735	2193-2196	MOP:0000780	denotes	cut
T6734	2114-2115	SO:0001031	denotes	R
T6733	2068-2069	SO:0001030	denotes	F
T6732	2048-2055	SO:0000112	denotes	Primers
T6731	1968-1987	GO:0001171	denotes	reverse transcribed
T6730	1709-1716,1721-1727	SO:0000132	denotes	reverse primer
T6728	1582-1588	SO:0000417	denotes	domain
T6727	1573-1577	PR:000012318	denotes	PAX6
T6726	1445-1462	SO:0000061	denotes	restriction sites
T6725	1325-1332	MOP:0000780	denotes	cutting
T6724	1261-1267	SO:0000417	denotes	domain
T6723	1229-1230	SO:0001031	denotes	R
T6722	1166-1167	SO:0001030	denotes	F
T6721	1146-1153	SO:0000112	denotes	Primers
T6720	1017-1018	SO:0001031	denotes	R

PMC:1208879 / 25288-31045 JSONTXT

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PMC:1208879 / 25288-31045 JSON TXT