Id |
Subject |
Object |
Predicate |
Lexical cue |
T8468 |
11-28 |
JJ |
denotes |
Semi-quantitative |
T8469 |
33-41 |
NN |
denotes |
analysis |
T8470 |
29-32 |
NN |
denotes |
PCR |
T8471 |
42-44 |
IN |
denotes |
of |
T8472 |
45-51 |
NN |
denotes |
Homer3 |
T8473 |
51-53 |
, |
denotes |
, |
T8474 |
53-58 |
NN |
denotes |
Dncl1 |
T8475 |
58-60 |
, |
denotes |
, |
T8476 |
60-66 |
NN |
denotes |
Trim11 |
T8477 |
67-70 |
CC |
denotes |
and |
T8478 |
71-75 |
NN |
denotes |
Pax6 |
T8479 |
76-78 |
IN |
denotes |
in |
T8480 |
79-82 |
DT |
denotes |
the |
T8481 |
100-107 |
NN |
denotes |
library |
T8482 |
83-88 |
NN |
denotes |
mouse |
T8483 |
89-94 |
NN |
denotes |
brain |
T8484 |
95-99 |
NN |
denotes |
cDNA |
T8485 |
107-108 |
. |
denotes |
. |
T8486 |
108-262 |
sentence |
denotes |
Library cDNA was amplified with primers specific for Pax6 (600 bp), Homer3 (485 bp band), Dncl1 (485 bp) and Trim11 (609 bp) for 20, 25, 30 or 35 cycles. |
T8487 |
109-116 |
NN |
denotes |
Library |
T8488 |
117-121 |
NN |
denotes |
cDNA |
T8489 |
126-135 |
VBN |
denotes |
amplified |
T8490 |
122-125 |
VBD |
denotes |
was |
T8491 |
136-140 |
IN |
denotes |
with |
T8492 |
141-148 |
NNS |
denotes |
primers |
T8493 |
149-157 |
JJ |
denotes |
specific |
T8494 |
158-161 |
IN |
denotes |
for |
T8495 |
162-166 |
NN |
denotes |
Pax6 |
T8496 |
167-168 |
-LRB- |
denotes |
( |
T8497 |
172-174 |
NN |
denotes |
bp |
T8498 |
168-171 |
CD |
denotes |
600 |
T8499 |
174-175 |
-RRB- |
denotes |
) |
T8500 |
175-177 |
, |
denotes |
, |
T8501 |
177-183 |
NN |
denotes |
Homer3 |
T8502 |
184-185 |
-LRB- |
denotes |
( |
T8503 |
192-196 |
NN |
denotes |
band |
T8504 |
185-188 |
CD |
denotes |
485 |
T8505 |
189-191 |
NN |
denotes |
bp |
T8506 |
196-197 |
-RRB- |
denotes |
) |
T8507 |
197-199 |
, |
denotes |
, |
T8508 |
199-204 |
NN |
denotes |
Dncl1 |
T8509 |
205-206 |
-LRB- |
denotes |
( |
T8510 |
210-212 |
NN |
denotes |
bp |
T8511 |
206-209 |
CD |
denotes |
485 |
T8512 |
212-213 |
-RRB- |
denotes |
) |
T8513 |
214-217 |
CC |
denotes |
and |
T8514 |
218-224 |
NN |
denotes |
Trim11 |
T8515 |
225-226 |
-LRB- |
denotes |
( |
T8516 |
230-232 |
NN |
denotes |
bp |
T8517 |
226-229 |
CD |
denotes |
609 |
T8518 |
232-233 |
-RRB- |
denotes |
) |
T8519 |
234-237 |
IN |
denotes |
for |
T8520 |
238-240 |
CD |
denotes |
20 |
T8521 |
255-261 |
NNS |
denotes |
cycles |
T8522 |
240-242 |
, |
denotes |
, |
T8523 |
242-244 |
CD |
denotes |
25 |
T8524 |
244-246 |
, |
denotes |
, |
T8525 |
246-248 |
CD |
denotes |
30 |
T8526 |
249-251 |
CC |
denotes |
or |
T8527 |
252-254 |
CD |
denotes |
35 |
T8528 |
261-262 |
. |
denotes |
. |
T8529 |
262-368 |
sentence |
denotes |
Atp5a1 (415 bp) and Gapdh (450 bp), which are highly expressed in the brain, are included for comparison. |
T8530 |
263-269 |
NN |
denotes |
Atp5a1 |
T8531 |
344-352 |
VBN |
denotes |
included |
T8532 |
270-271 |
-LRB- |
denotes |
( |
T8533 |
275-277 |
NN |
denotes |
bp |
T8534 |
271-274 |
CD |
denotes |
415 |
T8535 |
277-278 |
-RRB- |
denotes |
) |
T8536 |
279-282 |
CC |
denotes |
and |
T8537 |
283-288 |
NN |
denotes |
Gapdh |
T8538 |
289-290 |
-LRB- |
denotes |
( |
T8539 |
294-296 |
NN |
denotes |
bp |
T8540 |
290-293 |
CD |
denotes |
450 |
T8541 |
296-297 |
-RRB- |
denotes |
) |
T8542 |
297-299 |
, |
denotes |
, |
T8543 |
299-304 |
WDT |
denotes |
which |
T8544 |
316-325 |
VBN |
denotes |
expressed |
T8545 |
305-308 |
VBP |
denotes |
are |
T8546 |
309-315 |
RB |
denotes |
highly |
T8547 |
326-328 |
IN |
denotes |
in |
T8548 |
329-332 |
DT |
denotes |
the |
T8549 |
333-338 |
NN |
denotes |
brain |
T8550 |
338-340 |
, |
denotes |
, |
T8551 |
340-343 |
VBP |
denotes |
are |
T8552 |
353-356 |
IN |
denotes |
for |
T8553 |
357-367 |
NN |
denotes |
comparison |
T8554 |
367-368 |
. |
denotes |
. |
T8555 |
368-481 |
sentence |
denotes |
M indicates the Φ×174 HaeIII DNA size marker; the positions of the 603 bp and 310 bp marker bands are indicated. |
T8556 |
369-370 |
NN |
denotes |
M |
T8557 |
371-380 |
VBZ |
denotes |
indicates |
T8558 |
471-480 |
VBN |
denotes |
indicated |
T8559 |
381-384 |
DT |
denotes |
the |
T8560 |
407-413 |
NN |
denotes |
marker |
T8561 |
385-387 |
NN |
denotes |
Φ× |
T8562 |
391-397 |
NN |
denotes |
HaeIII |
T8563 |
387-390 |
CD |
denotes |
174 |
T8564 |
398-401 |
NN |
denotes |
DNA |
T8565 |
402-406 |
NN |
denotes |
size |
T8566 |
413-414 |
: |
denotes |
; |
T8567 |
415-418 |
DT |
denotes |
the |
T8568 |
419-428 |
NNS |
denotes |
positions |
T8569 |
429-431 |
IN |
denotes |
of |
T8570 |
432-435 |
DT |
denotes |
the |
T8571 |
461-466 |
NNS |
denotes |
bands |
T8572 |
436-439 |
CD |
denotes |
603 |
T8573 |
440-442 |
NN |
denotes |
bp |
T8574 |
443-446 |
CC |
denotes |
and |
T8575 |
447-450 |
CD |
denotes |
310 |
T8576 |
451-453 |
NN |
denotes |
bp |
T8577 |
454-460 |
NN |
denotes |
marker |
T8578 |
467-470 |
VBP |
denotes |
are |
T8579 |
480-481 |
. |
denotes |
. |
T2968 |
0-572 |
sentence |
denotes |
Figure 2 Semi-quantitative PCR analysis of Homer3, Dncl1, Trim11 and Pax6 in the mouse brain cDNA library. Library cDNA was amplified with primers specific for Pax6 (600 bp), Homer3 (485 bp band), Dncl1 (485 bp) and Trim11 (609 bp) for 20, 25, 30 or 35 cycles. Atp5a1 (415 bp) and Gapdh (450 bp), which are highly expressed in the brain, are included for comparison. M indicates the Φ×174 HaeIII DNA size marker; the positions of the 603 bp and 310 bp marker bands are indicated. We concluded that Homer3, Dncl1 and Trim11 clones were not highly abundant in the library. |
T2969 |
482-484 |
PRP |
denotes |
We |
T2970 |
485-494 |
VBD |
denotes |
concluded |
T2971 |
495-499 |
IN |
denotes |
that |
T2972 |
532-536 |
VBD |
denotes |
were |
T2973 |
500-506 |
NN |
denotes |
Homer3 |
T2974 |
525-531 |
NNS |
denotes |
clones |
T2975 |
506-508 |
, |
denotes |
, |
T2976 |
508-513 |
NN |
denotes |
Dncl1 |
T2977 |
514-517 |
CC |
denotes |
and |
T2978 |
518-524 |
NN |
denotes |
Trim11 |
T2979 |
537-540 |
RB |
denotes |
not |
T2980 |
541-547 |
RB |
denotes |
highly |
T2981 |
548-556 |
JJ |
denotes |
abundant |
T2982 |
557-559 |
IN |
denotes |
in |
T2983 |
560-563 |
DT |
denotes |
the |
T2984 |
564-571 |
NN |
denotes |
library |
T2985 |
571-572 |
. |
denotes |
. |
R1869 |
T2972 |
T2970 |
ccomp |
were,concluded |
R1871 |
T2974 |
T2972 |
nsubj |
clones,were |
R1873 |
T2976 |
T2973 |
conj |
Dncl1,Homer3 |
R1874 |
T2977 |
T2976 |
cc |
and,Dncl1 |
R1875 |
T2978 |
T2976 |
conj |
Trim11,Dncl1 |
R1876 |
T2979 |
T2972 |
neg |
not,were |
R1877 |
T2980 |
T2981 |
advmod |
highly,abundant |
R1878 |
T2981 |
T2972 |
acomp |
abundant,were |
R1879 |
T2982 |
T2972 |
prep |
in,were |
R1880 |
T2983 |
T2984 |
det |
the,library |
R1881 |
T2984 |
T2982 |
pobj |
library,in |
R1882 |
T2985 |
T2970 |
punct |
.,concluded |
R5394 |
T8468 |
T8469 |
amod |
Semi-quantitative,analysis |
R5395 |
T8470 |
T8469 |
compound |
PCR,analysis |
R5396 |
T8471 |
T8469 |
prep |
of,analysis |
R5397 |
T8472 |
T8471 |
pobj |
Homer3,of |
R5398 |
T8473 |
T8472 |
punct |
", ",Homer3 |
R5399 |
T8474 |
T8472 |
conj |
Dncl1,Homer3 |
R5400 |
T8475 |
T8474 |
punct |
", ",Dncl1 |
R5401 |
T8476 |
T8474 |
conj |
Trim11,Dncl1 |
R5402 |
T8477 |
T8476 |
cc |
and,Trim11 |
R5403 |
T8478 |
T8476 |
conj |
Pax6,Trim11 |
R5404 |
T8479 |
T8469 |
prep |
in,analysis |
R5405 |
T8480 |
T8481 |
det |
the,library |
R5406 |
T8481 |
T8479 |
pobj |
library,in |
R5407 |
T8482 |
T8483 |
compound |
mouse,brain |
R5408 |
T8483 |
T8481 |
compound |
brain,library |
R5409 |
T8484 |
T8481 |
compound |
cDNA,library |
R5410 |
T8485 |
T8469 |
punct |
.,analysis |
R5411 |
T8487 |
T8488 |
compound |
Library,cDNA |
R5412 |
T8488 |
T8489 |
nsubjpass |
cDNA,amplified |
R5413 |
T8490 |
T8489 |
auxpass |
was,amplified |
R5414 |
T8491 |
T8489 |
prep |
with,amplified |
R5415 |
T8492 |
T8491 |
pobj |
primers,with |
R5416 |
T8493 |
T8492 |
amod |
specific,primers |
R5417 |
T8494 |
T8493 |
prep |
for,specific |
R5418 |
T8495 |
T8494 |
pobj |
Pax6,for |
R5419 |
T8496 |
T8497 |
punct |
(,bp |
R5420 |
T8497 |
T8495 |
parataxis |
bp,Pax6 |
R5421 |
T8498 |
T8497 |
nummod |
600,bp |
R5422 |
T8499 |
T8497 |
punct |
),bp |
R5423 |
T8500 |
T8495 |
punct |
", ",Pax6 |
R5424 |
T8501 |
T8495 |
conj |
Homer3,Pax6 |
R5425 |
T8502 |
T8503 |
punct |
(,band |
R5426 |
T8503 |
T8501 |
parataxis |
band,Homer3 |
R5427 |
T8504 |
T8505 |
nummod |
485,bp |
R5428 |
T8505 |
T8503 |
compound |
bp,band |
R5429 |
T8506 |
T8503 |
punct |
),band |
R5430 |
T8507 |
T8501 |
punct |
", ",Homer3 |
R5431 |
T8508 |
T8501 |
conj |
Dncl1,Homer3 |
R5432 |
T8509 |
T8510 |
punct |
(,bp |
R5433 |
T8510 |
T8508 |
parataxis |
bp,Dncl1 |
R5434 |
T8511 |
T8510 |
nummod |
485,bp |
R5435 |
T8512 |
T8510 |
punct |
),bp |
R5436 |
T8513 |
T8508 |
cc |
and,Dncl1 |
R5437 |
T8514 |
T8508 |
conj |
Trim11,Dncl1 |
R5438 |
T8515 |
T8516 |
punct |
(,bp |
R5439 |
T8516 |
T8514 |
parataxis |
bp,Trim11 |
R5440 |
T8517 |
T8516 |
nummod |
609,bp |
R5441 |
T8518 |
T8516 |
punct |
),bp |
R5442 |
T8519 |
T8489 |
prep |
for,amplified |
R5443 |
T8520 |
T8521 |
nummod |
20,cycles |
R5444 |
T8521 |
T8519 |
pobj |
cycles,for |
R5445 |
T8522 |
T8520 |
punct |
", ",20 |
R5446 |
T8523 |
T8520 |
conj |
25,20 |
R5447 |
T8524 |
T8523 |
punct |
", ",25 |
R5448 |
T8525 |
T8523 |
conj |
30,25 |
R5449 |
T8526 |
T8525 |
cc |
or,30 |
R5450 |
T8527 |
T8525 |
conj |
35,30 |
R5451 |
T8528 |
T8489 |
punct |
.,amplified |
R5452 |
T8530 |
T8531 |
nsubjpass |
Atp5a1,included |
R5453 |
T8532 |
T8533 |
punct |
(,bp |
R5454 |
T8533 |
T8530 |
parataxis |
bp,Atp5a1 |
R5455 |
T8534 |
T8533 |
nummod |
415,bp |
R5456 |
T8535 |
T8533 |
punct |
),bp |
R5457 |
T8536 |
T8530 |
cc |
and,Atp5a1 |
R5458 |
T8537 |
T8530 |
conj |
Gapdh,Atp5a1 |
R5459 |
T8538 |
T8539 |
punct |
(,bp |
R5460 |
T8539 |
T8537 |
parataxis |
bp,Gapdh |
R5461 |
T8540 |
T8539 |
nummod |
450,bp |
R5462 |
T8541 |
T8539 |
punct |
),bp |
R5463 |
T8542 |
T8530 |
punct |
", ",Atp5a1 |
R5464 |
T8543 |
T8544 |
dep |
which,expressed |
R5465 |
T8544 |
T8530 |
relcl |
expressed,Atp5a1 |
R5466 |
T8545 |
T8544 |
auxpass |
are,expressed |
R5467 |
T8546 |
T8544 |
advmod |
highly,expressed |
R5468 |
T8547 |
T8544 |
prep |
in,expressed |
R5469 |
T8548 |
T8549 |
det |
the,brain |
R5470 |
T8549 |
T8547 |
pobj |
brain,in |
R5471 |
T8550 |
T8531 |
punct |
", ",included |
R5472 |
T8551 |
T8531 |
auxpass |
are,included |
R5473 |
T8552 |
T8531 |
prep |
for,included |
R5474 |
T8553 |
T8552 |
pobj |
comparison,for |
R5475 |
T8554 |
T8531 |
punct |
.,included |
R5476 |
T8556 |
T8557 |
nsubj |
M,indicates |
R5477 |
T8557 |
T8558 |
ccomp |
indicates,indicated |
R5478 |
T8559 |
T8560 |
det |
the,marker |
R5479 |
T8560 |
T8557 |
dobj |
marker,indicates |
R5480 |
T8561 |
T8562 |
nmod |
Φ×,HaeIII |
R5481 |
T8562 |
T8560 |
compound |
HaeIII,marker |
R5482 |
T8563 |
T8562 |
nummod |
174,HaeIII |
R5483 |
T8564 |
T8565 |
compound |
DNA,size |
R5484 |
T8565 |
T8560 |
compound |
size,marker |
R5485 |
T8566 |
T8558 |
punct |
;,indicated |
R5486 |
T8567 |
T8568 |
det |
the,positions |
R5487 |
T8568 |
T8558 |
nsubjpass |
positions,indicated |
R5488 |
T8569 |
T8568 |
prep |
of,positions |
R5489 |
T8570 |
T8571 |
det |
the,bands |
R5490 |
T8571 |
T8569 |
pobj |
bands,of |
R5491 |
T8572 |
T8573 |
nummod |
603,bp |
R5492 |
T8573 |
T8571 |
nmod |
bp,bands |
R5493 |
T8574 |
T8573 |
cc |
and,bp |
R5494 |
T8575 |
T8576 |
nummod |
310,bp |
R5495 |
T8576 |
T8573 |
conj |
bp,bp |
R5496 |
T8577 |
T8571 |
compound |
marker,bands |
R5497 |
T8578 |
T8558 |
auxpass |
are,indicated |
R5498 |
T8579 |
T8558 |
punct |
.,indicated |
R1867 |
T2969 |
T2970 |
nsubj |
We,concluded |
R1868 |
T2971 |
T2972 |
mark |
that,were |
R1870 |
T2973 |
T2974 |
nmod |
Homer3,clones |
R1872 |
T2975 |
T2973 |
punct |
", ",Homer3 |