Id |
Subject |
Object |
Predicate |
Lexical cue |
T7725 |
9-13 |
NNS |
denotes |
Mice |
T7726 |
14-17 |
CC |
denotes |
and |
T7727 |
18-28 |
NN |
denotes |
genotyping |
T7728 |
28-176 |
sentence |
denotes |
The Hdhex4/5 mice carrying a pGKneo insertion/replacement inactivating mutation in the mouse HD gene homologue have been described previously [10]. |
T7729 |
29-32 |
DT |
denotes |
The |
T7730 |
42-46 |
NNS |
denotes |
mice |
T7731 |
33-39 |
NN |
denotes |
Hdhex4 |
T7732 |
39-40 |
HYPH |
denotes |
/ |
T7733 |
40-41 |
CD |
denotes |
5 |
T7734 |
150-159 |
VBN |
denotes |
described |
T7735 |
47-55 |
VBG |
denotes |
carrying |
T7736 |
56-57 |
DT |
denotes |
a |
T7737 |
100-108 |
NN |
denotes |
mutation |
T7738 |
58-64 |
NN |
denotes |
pGKneo |
T7739 |
75-86 |
NN |
denotes |
replacement |
T7740 |
65-74 |
NN |
denotes |
insertion |
T7741 |
74-75 |
HYPH |
denotes |
/ |
T7742 |
87-99 |
VBG |
denotes |
inactivating |
T7743 |
109-111 |
IN |
denotes |
in |
T7744 |
112-115 |
DT |
denotes |
the |
T7745 |
130-139 |
NN |
denotes |
homologue |
T7746 |
116-121 |
NN |
denotes |
mouse |
T7747 |
122-124 |
NN |
denotes |
HD |
T7748 |
125-129 |
NN |
denotes |
gene |
T7749 |
140-144 |
VBP |
denotes |
have |
T7750 |
145-149 |
VBN |
denotes |
been |
T7751 |
160-170 |
RB |
denotes |
previously |
T7752 |
171-172 |
-LRB- |
denotes |
[ |
T7753 |
172-174 |
CD |
denotes |
10 |
T7754 |
174-175 |
-RRB- |
denotes |
] |
T7755 |
175-176 |
. |
denotes |
. |
T7756 |
176-303 |
sentence |
denotes |
The experiments were conducted in accordance with an IACUC approved protocol, through the MGH Subcommittee on Animal Research. |
T7757 |
177-180 |
DT |
denotes |
The |
T7758 |
181-192 |
NNS |
denotes |
experiments |
T7759 |
198-207 |
VBN |
denotes |
conducted |
T7760 |
193-197 |
VBD |
denotes |
were |
T7761 |
208-210 |
IN |
denotes |
in |
T7762 |
211-221 |
NN |
denotes |
accordance |
T7763 |
222-226 |
IN |
denotes |
with |
T7764 |
227-229 |
DT |
denotes |
an |
T7765 |
245-253 |
NN |
denotes |
protocol |
T7766 |
230-235 |
NNP |
denotes |
IACUC |
T7767 |
236-244 |
VBN |
denotes |
approved |
T7768 |
253-255 |
, |
denotes |
, |
T7769 |
255-262 |
IN |
denotes |
through |
T7770 |
263-266 |
DT |
denotes |
the |
T7771 |
271-283 |
NNP |
denotes |
Subcommittee |
T7772 |
267-270 |
NNP |
denotes |
MGH |
T7773 |
284-286 |
IN |
denotes |
on |
T7774 |
287-293 |
NNP |
denotes |
Animal |
T7775 |
294-302 |
NNP |
denotes |
Research |
T7776 |
302-303 |
. |
denotes |
. |
T7777 |
303-473 |
sentence |
denotes |
Mutant Hdhex4/5/Hdhex4/5 and normal littermates were obtained in timed pregnancies from mating of Hdhex4/5/Hdh+ heterozygotes, genotyped by PCR assay, as described [10]. |
T7778 |
304-310 |
NN |
denotes |
Mutant |
T7779 |
311-317 |
NN |
denotes |
Hdhex4 |
T7780 |
340-351 |
NNS |
denotes |
littermates |
T7781 |
317-318 |
HYPH |
denotes |
/ |
T7782 |
318-319 |
CD |
denotes |
5 |
T7783 |
319-320 |
HYPH |
denotes |
/ |
T7784 |
320-326 |
NN |
denotes |
Hdhex4 |
T7785 |
326-327 |
HYPH |
denotes |
/ |
T7786 |
327-328 |
CD |
denotes |
5 |
T7787 |
329-332 |
CC |
denotes |
and |
T7788 |
333-339 |
JJ |
denotes |
normal |
T7789 |
357-365 |
VBN |
denotes |
obtained |
T7790 |
352-356 |
VBD |
denotes |
were |
T7791 |
366-368 |
IN |
denotes |
in |
T7792 |
369-374 |
VBN |
denotes |
timed |
T7793 |
375-386 |
NNS |
denotes |
pregnancies |
T7794 |
387-391 |
IN |
denotes |
from |
T7795 |
392-398 |
NN |
denotes |
mating |
T7796 |
399-401 |
IN |
denotes |
of |
T7797 |
402-408 |
NN |
denotes |
Hdhex4 |
T7798 |
416-429 |
NNS |
denotes |
heterozygotes |
T7799 |
408-409 |
HYPH |
denotes |
/ |
T7800 |
409-410 |
CD |
denotes |
5 |
T7801 |
410-411 |
HYPH |
denotes |
/ |
T7802 |
411-414 |
NN |
denotes |
Hdh |
T7803 |
414-415 |
SYM |
denotes |
+ |
T7804 |
429-431 |
, |
denotes |
, |
T7805 |
431-440 |
VBN |
denotes |
genotyped |
T7806 |
441-443 |
IN |
denotes |
by |
T7807 |
444-447 |
NN |
denotes |
PCR |
T7808 |
448-453 |
NN |
denotes |
assay |
T7809 |
453-455 |
, |
denotes |
, |
T7810 |
455-457 |
IN |
denotes |
as |
T7811 |
458-467 |
VBN |
denotes |
described |
T7812 |
468-469 |
-LRB- |
denotes |
[ |
T7813 |
469-471 |
CD |
denotes |
10 |
T7814 |
471-472 |
-RRB- |
denotes |
] |
T7815 |
472-473 |
. |
denotes |
. |
T7816 |
473-511 |
sentence |
denotes |
The day of plug was taken to be E0.5. |
T7817 |
474-477 |
DT |
denotes |
The |
T7818 |
478-481 |
NN |
denotes |
day |
T7819 |
494-499 |
VBN |
denotes |
taken |
T7820 |
482-484 |
IN |
denotes |
of |
T7821 |
485-489 |
NN |
denotes |
plug |
T7822 |
490-493 |
VBD |
denotes |
was |
T7823 |
500-502 |
TO |
denotes |
to |
T7824 |
503-505 |
VB |
denotes |
be |
T7825 |
506-510 |
NN |
denotes |
E0.5 |
T7826 |
510-511 |
. |
denotes |
. |
T7827 |
511-625 |
sentence |
denotes |
Embryos that were morphologically normal were pooled separately from morphologically mutant embryos for analysis. |
T7828 |
512-519 |
NNS |
denotes |
Embryos |
T7829 |
558-564 |
VBN |
denotes |
pooled |
T7830 |
520-524 |
WDT |
denotes |
that |
T7831 |
525-529 |
VBD |
denotes |
were |
T7832 |
530-545 |
RB |
denotes |
morphologically |
T7833 |
546-552 |
JJ |
denotes |
normal |
T7834 |
553-557 |
VBD |
denotes |
were |
T7835 |
565-575 |
RB |
denotes |
separately |
T7836 |
576-580 |
IN |
denotes |
from |
T7837 |
581-596 |
RB |
denotes |
morphologically |
T7838 |
597-603 |
NN |
denotes |
mutant |
T7839 |
604-611 |
NNS |
denotes |
embryos |
T7840 |
612-615 |
IN |
denotes |
for |
T7841 |
616-624 |
NN |
denotes |
analysis |
T7842 |
624-625 |
. |
denotes |
. |
T7843 |
625-780 |
sentence |
denotes |
Nodal expression was determined in embryos from matings of Hdhex4/5/Hdh+; NdllacZ/Ndl+ compound heterozygotes genotyped by PCR assay as described in [29]. |
T7844 |
626-631 |
NN |
denotes |
Nodal |
T7845 |
632-642 |
NN |
denotes |
expression |
T7846 |
647-657 |
VBN |
denotes |
determined |
T7847 |
643-646 |
VBD |
denotes |
was |
T7848 |
658-660 |
IN |
denotes |
in |
T7849 |
661-668 |
NNS |
denotes |
embryos |
T7850 |
669-673 |
IN |
denotes |
from |
T7851 |
674-681 |
NNS |
denotes |
matings |
T7852 |
682-684 |
IN |
denotes |
of |
T7853 |
685-691 |
NN |
denotes |
Hdhex4 |
T7854 |
722-735 |
NNS |
denotes |
heterozygotes |
T7855 |
691-692 |
HYPH |
denotes |
/ |
T7856 |
692-693 |
CD |
denotes |
5 |
T7857 |
693-694 |
HYPH |
denotes |
/ |
T7858 |
694-697 |
NN |
denotes |
Hdh |
T7859 |
697-698 |
SYM |
denotes |
+ |
T7860 |
698-699 |
, |
denotes |
; |
T7861 |
700-707 |
NN |
denotes |
NdllacZ |
T7862 |
708-711 |
NN |
denotes |
Ndl |
T7863 |
707-708 |
HYPH |
denotes |
/ |
T7864 |
711-712 |
SYM |
denotes |
+ |
T7865 |
713-721 |
NN |
denotes |
compound |
T7866 |
736-745 |
VBN |
denotes |
genotyped |
T7867 |
746-748 |
IN |
denotes |
by |
T7868 |
749-752 |
NN |
denotes |
PCR |
T7869 |
753-758 |
NN |
denotes |
assay |
T7870 |
759-761 |
IN |
denotes |
as |
T7871 |
762-771 |
VBN |
denotes |
described |
T7872 |
772-774 |
IN |
denotes |
in |
T7873 |
775-776 |
-LRB- |
denotes |
[ |
T7874 |
776-778 |
CD |
denotes |
29 |
T7875 |
778-779 |
-RRB- |
denotes |
] |
T7876 |
779-780 |
. |
denotes |
. |
T7923 |
782-787 |
JJ |
denotes |
Whole |
T7924 |
788-793 |
NN |
denotes |
mount |
T7925 |
814-827 |
NN |
denotes |
hybridization |
T7926 |
794-797 |
CC |
denotes |
and |
T7927 |
798-805 |
NN |
denotes |
section |
T7928 |
806-808 |
FW |
denotes |
in |
T7929 |
809-813 |
FW |
denotes |
situ |
T7930 |
828-831 |
CC |
denotes |
and |
T7931 |
832-833 |
NN |
denotes |
β |
T7932 |
834-837 |
NN |
denotes |
gal |
T7933 |
833-834 |
HYPH |
denotes |
- |
T7934 |
838-846 |
NN |
denotes |
staining |
T7935 |
846-931 |
sentence |
denotes |
After dissection in PBS, embryos were fixed overnight in 4% paraformaldehyde at 4°C. |
T7936 |
847-852 |
IN |
denotes |
After |
T7937 |
885-890 |
VBN |
denotes |
fixed |
T7938 |
853-863 |
NN |
denotes |
dissection |
T7939 |
864-866 |
IN |
denotes |
in |
T7940 |
867-870 |
NN |
denotes |
PBS |
T7941 |
870-872 |
, |
denotes |
, |
T7942 |
872-879 |
NNS |
denotes |
embryos |
T7943 |
880-884 |
VBD |
denotes |
were |
T7944 |
891-900 |
RB |
denotes |
overnight |
T7945 |
901-903 |
IN |
denotes |
in |
T7946 |
904-905 |
CD |
denotes |
4 |
T7947 |
905-906 |
NN |
denotes |
% |
T7948 |
907-923 |
NN |
denotes |
paraformaldehyde |
T7949 |
924-926 |
IN |
denotes |
at |
T7950 |
927-928 |
CD |
denotes |
4 |
T7951 |
928-930 |
NN |
denotes |
°C |
T7952 |
930-931 |
. |
denotes |
. |
T7953 |
931-1037 |
sentence |
denotes |
For sections, decidua fixed in 4% paraformaldehyde, were embedded in paraffin and sectioned at 7 microns. |
T7954 |
932-935 |
IN |
denotes |
For |
T7955 |
989-997 |
VBN |
denotes |
embedded |
T7956 |
936-944 |
NNS |
denotes |
sections |
T7957 |
944-946 |
, |
denotes |
, |
T7958 |
946-953 |
NNS |
denotes |
decidua |
T7959 |
954-959 |
VBN |
denotes |
fixed |
T7960 |
960-962 |
IN |
denotes |
in |
T7961 |
963-964 |
CD |
denotes |
4 |
T7962 |
964-965 |
NN |
denotes |
% |
T7963 |
966-982 |
NN |
denotes |
paraformaldehyde |
T7964 |
982-984 |
, |
denotes |
, |
T7965 |
984-988 |
VBD |
denotes |
were |
T7966 |
998-1000 |
IN |
denotes |
in |
T7967 |
1001-1009 |
NN |
denotes |
paraffin |
T7968 |
1010-1013 |
CC |
denotes |
and |
T7969 |
1014-1023 |
VBN |
denotes |
sectioned |
T7970 |
1024-1026 |
IN |
denotes |
at |
T7971 |
1027-1028 |
CD |
denotes |
7 |
T7972 |
1029-1036 |
NNS |
denotes |
microns |
T7973 |
1036-1037 |
. |
denotes |
. |
T7974 |
1037-1109 |
sentence |
denotes |
RNA in situ hybridizations were performed as described previously [55]. |
T7975 |
1038-1041 |
NN |
denotes |
RNA |
T7976 |
1050-1064 |
NNS |
denotes |
hybridizations |
T7977 |
1042-1044 |
FW |
denotes |
in |
T7978 |
1045-1049 |
FW |
denotes |
situ |
T7979 |
1070-1079 |
VBN |
denotes |
performed |
T7980 |
1065-1069 |
VBD |
denotes |
were |
T7981 |
1080-1082 |
IN |
denotes |
as |
T7982 |
1083-1092 |
VBN |
denotes |
described |
T7983 |
1093-1103 |
RB |
denotes |
previously |
T7984 |
1104-1105 |
-LRB- |
denotes |
[ |
T7985 |
1105-1107 |
CD |
denotes |
55 |
T7986 |
1107-1108 |
-RRB- |
denotes |
] |
T7987 |
1108-1109 |
. |
denotes |
. |
T7988 |
1109-1236 |
sentence |
denotes |
Nodal.lacZ expression was assessed by β-galactosidase staining as reported [29], on embryos post fixed in 4% paraformaldehyde. |
T7989 |
1110-1115 |
NN |
denotes |
Nodal |
T7990 |
1116-1120 |
NN |
denotes |
lacZ |
T7991 |
1115-1116 |
HYPH |
denotes |
. |
T7992 |
1121-1131 |
NN |
denotes |
expression |
T7993 |
1136-1144 |
VBN |
denotes |
assessed |
T7994 |
1132-1135 |
VBD |
denotes |
was |
T7995 |
1145-1147 |
IN |
denotes |
by |
T7996 |
1148-1149 |
NN |
denotes |
β |
T7997 |
1150-1163 |
NN |
denotes |
galactosidase |
T7998 |
1149-1150 |
HYPH |
denotes |
- |
T7999 |
1164-1172 |
NN |
denotes |
staining |
T8000 |
1173-1175 |
IN |
denotes |
as |
T8001 |
1176-1184 |
VBN |
denotes |
reported |
T8002 |
1185-1186 |
-LRB- |
denotes |
[ |
T8003 |
1186-1188 |
CD |
denotes |
29 |
T8004 |
1188-1189 |
-RRB- |
denotes |
] |
T8005 |
1189-1191 |
, |
denotes |
, |
T8006 |
1191-1193 |
IN |
denotes |
on |
T8007 |
1194-1201 |
NNS |
denotes |
embryos |
T8008 |
1202-1206 |
AFX |
denotes |
post |
T8009 |
1207-1212 |
VBN |
denotes |
fixed |
T8010 |
1213-1215 |
IN |
denotes |
in |
T8011 |
1216-1217 |
CD |
denotes |
4 |
T8012 |
1217-1218 |
NN |
denotes |
% |
T8013 |
1219-1235 |
NN |
denotes |
paraformaldehyde |
T8014 |
1235-1236 |
. |
denotes |
. |
T8015 |
1236-1300 |
sentence |
denotes |
Embryos were mounted in 80% glycerol before being photographed. |
T8016 |
1237-1244 |
NNS |
denotes |
Embryos |
T8017 |
1250-1257 |
VBN |
denotes |
mounted |
T8018 |
1245-1249 |
VBD |
denotes |
were |
T8019 |
1258-1260 |
IN |
denotes |
in |
T8020 |
1261-1263 |
CD |
denotes |
80 |
T8021 |
1263-1264 |
NN |
denotes |
% |
T8022 |
1265-1273 |
NN |
denotes |
glycerol |
T8023 |
1274-1280 |
IN |
denotes |
before |
T8024 |
1281-1286 |
VBG |
denotes |
being |
T8025 |
1287-1299 |
VBN |
denotes |
photographed |
T8026 |
1299-1300 |
. |
denotes |
. |
T8027 |
1300-1401 |
sentence |
denotes |
The huntingtin deficient phenotype is fully penetrant at each of the stages that were assessed [10]. |
T8028 |
1301-1304 |
DT |
denotes |
The |
T8029 |
1326-1335 |
NN |
denotes |
phenotype |
T8030 |
1305-1315 |
NN |
denotes |
huntingtin |
T8031 |
1316-1325 |
JJ |
denotes |
deficient |
T8032 |
1336-1338 |
VBZ |
denotes |
is |
T8033 |
1339-1344 |
RB |
denotes |
fully |
T8034 |
1345-1354 |
JJ |
denotes |
penetrant |
T8035 |
1355-1357 |
IN |
denotes |
at |
T8036 |
1358-1362 |
DT |
denotes |
each |
T8037 |
1363-1365 |
IN |
denotes |
of |
T8038 |
1366-1369 |
DT |
denotes |
the |
T8039 |
1370-1376 |
NNS |
denotes |
stages |
T8040 |
1377-1381 |
WDT |
denotes |
that |
T8041 |
1387-1395 |
VBN |
denotes |
assessed |
T8042 |
1382-1386 |
VBD |
denotes |
were |
T8043 |
1396-1397 |
-LRB- |
denotes |
[ |
T8044 |
1397-1399 |
CD |
denotes |
10 |
T8045 |
1399-1400 |
-RRB- |
denotes |
] |
T8046 |
1400-1401 |
. |
denotes |
. |
T8047 |
1401-1523 |
sentence |
denotes |
Three to six embryos were evaluated for each marker, with every embryo exhibiting the same mutant phenotype in each case. |
T8048 |
1402-1407 |
CD |
denotes |
Three |
T8049 |
1411-1414 |
CD |
denotes |
six |
T8050 |
1408-1410 |
IN |
denotes |
to |
T8051 |
1415-1422 |
NNS |
denotes |
embryos |
T8052 |
1428-1437 |
VBN |
denotes |
evaluated |
T8053 |
1423-1427 |
VBD |
denotes |
were |
T8054 |
1438-1441 |
IN |
denotes |
for |
T8055 |
1442-1446 |
DT |
denotes |
each |
T8056 |
1447-1453 |
NN |
denotes |
marker |
T8057 |
1453-1455 |
, |
denotes |
, |
T8058 |
1455-1459 |
IN |
denotes |
with |
T8059 |
1460-1465 |
DT |
denotes |
every |
T8060 |
1466-1472 |
NN |
denotes |
embryo |
T8061 |
1473-1483 |
VBG |
denotes |
exhibiting |
T8062 |
1484-1487 |
DT |
denotes |
the |
T8063 |
1500-1509 |
NN |
denotes |
phenotype |
T8064 |
1488-1492 |
JJ |
denotes |
same |
T8065 |
1493-1499 |
NN |
denotes |
mutant |
T8066 |
1510-1512 |
IN |
denotes |
in |
T8067 |
1513-1517 |
DT |
denotes |
each |
T8068 |
1518-1522 |
NN |
denotes |
case |
T8069 |
1522-1523 |
. |
denotes |
. |
T8127 |
1534-1545 |
NN |
denotes |
phosphatase |
T8128 |
1546-1554 |
NN |
denotes |
staining |
T8129 |
1555-1557 |
IN |
denotes |
of |
T8130 |
1558-1568 |
JJ |
denotes |
Primordial |
T8131 |
1574-1579 |
NNS |
denotes |
Cells |
T8132 |
1569-1573 |
NN |
denotes |
Germ |
T8133 |
1580-1581 |
-LRB- |
denotes |
( |
T8134 |
1581-1585 |
NNS |
denotes |
PCGs |
T8135 |
1585-1586 |
-RRB- |
denotes |
) |
T8136 |
1586-1708 |
sentence |
denotes |
After dissections, embryos were fixed in 4% paraformaldehyde briefly and washed and stored in 1 × PBS/0.1% TX-100 at 4°C. |
T8137 |
1587-1592 |
IN |
denotes |
After |
T8138 |
1619-1624 |
VBN |
denotes |
fixed |
T8139 |
1593-1604 |
NNS |
denotes |
dissections |
T8140 |
1604-1606 |
, |
denotes |
, |
T8141 |
1606-1613 |
NNS |
denotes |
embryos |
T8142 |
1614-1618 |
VBD |
denotes |
were |
T8143 |
1625-1627 |
IN |
denotes |
in |
T8144 |
1628-1629 |
CD |
denotes |
4 |
T8145 |
1629-1630 |
NN |
denotes |
% |
T8146 |
1631-1647 |
NN |
denotes |
paraformaldehyde |
T8147 |
1648-1655 |
RB |
denotes |
briefly |
T8148 |
1656-1659 |
CC |
denotes |
and |
T8149 |
1660-1666 |
VBN |
denotes |
washed |
T8150 |
1667-1670 |
CC |
denotes |
and |
T8151 |
1671-1677 |
VBN |
denotes |
stored |
T8152 |
1678-1680 |
IN |
denotes |
in |
T8153 |
1681-1682 |
CD |
denotes |
1 |
T8154 |
1685-1688 |
NN |
denotes |
PBS |
T8155 |
1683-1684 |
SYM |
denotes |
× |
T8156 |
1688-1689 |
HYPH |
denotes |
/ |
T8157 |
1689-1692 |
NN |
denotes |
0.1 |
T8158 |
1692-1693 |
NN |
denotes |
% |
T8159 |
1694-1696 |
NN |
denotes |
TX |
T8160 |
1696-1697 |
HYPH |
denotes |
- |
T8161 |
1697-1700 |
CD |
denotes |
100 |
T8162 |
1701-1703 |
IN |
denotes |
at |
T8163 |
1704-1705 |
CD |
denotes |
4 |
T8164 |
1705-1707 |
NN |
denotes |
°C |
T8165 |
1707-1708 |
. |
denotes |
. |
T8166 |
1708-1977 |
sentence |
denotes |
Embryos were washed once with Tris-Maleate Buffer (25 mM Tris-Maleate, pH = 9.0, 0.8 mM MgCl2) and were subsequently incubated in alkaline phosphatase staining solution (25 mM Tris-Maleate, pH = 9.0, 0.8 mM MgCl2, 0.4 mg/ml alpha-naphthyl phosphate, 1 mg/ml Fast-Red). |
T8167 |
1709-1716 |
NNS |
denotes |
Embryos |
T8168 |
1722-1728 |
VBN |
denotes |
washed |
T8169 |
1717-1721 |
VBD |
denotes |
were |
T8170 |
1729-1733 |
RB |
denotes |
once |
T8171 |
1734-1738 |
IN |
denotes |
with |
T8172 |
1739-1743 |
NN |
denotes |
Tris |
T8173 |
1744-1751 |
NN |
denotes |
Maleate |
T8174 |
1743-1744 |
HYPH |
denotes |
- |
T8175 |
1752-1758 |
NN |
denotes |
Buffer |
T8176 |
1759-1760 |
-LRB- |
denotes |
( |
T8177 |
1797-1802 |
NN |
denotes |
MgCl2 |
T8178 |
1760-1762 |
CD |
denotes |
25 |
T8179 |
1763-1765 |
NN |
denotes |
mM |
T8180 |
1771-1778 |
NN |
denotes |
Maleate |
T8181 |
1766-1770 |
NN |
denotes |
Tris |
T8182 |
1770-1771 |
HYPH |
denotes |
- |
T8183 |
1778-1780 |
, |
denotes |
, |
T8184 |
1780-1782 |
NN |
denotes |
pH |
T8185 |
1785-1788 |
CD |
denotes |
9.0 |
T8186 |
1783-1784 |
SYM |
denotes |
= |
T8187 |
1788-1790 |
, |
denotes |
, |
T8188 |
1790-1793 |
CD |
denotes |
0.8 |
T8189 |
1794-1796 |
NN |
denotes |
mM |
T8190 |
1802-1803 |
-RRB- |
denotes |
) |
T8191 |
1804-1807 |
CC |
denotes |
and |
T8192 |
1808-1812 |
VBD |
denotes |
were |
T8193 |
1826-1835 |
VBN |
denotes |
incubated |
T8194 |
1813-1825 |
RB |
denotes |
subsequently |
T8195 |
1836-1838 |
IN |
denotes |
in |
T8196 |
1839-1847 |
NN |
denotes |
alkaline |
T8197 |
1848-1859 |
NN |
denotes |
phosphatase |
T8198 |
1869-1877 |
NN |
denotes |
solution |
T8199 |
1860-1868 |
NN |
denotes |
staining |
T8200 |
1878-1879 |
-LRB- |
denotes |
( |
T8201 |
1961-1963 |
NN |
denotes |
mg |
T8202 |
1879-1881 |
CD |
denotes |
25 |
T8203 |
1882-1884 |
NN |
denotes |
mM |
T8204 |
1890-1897 |
NN |
denotes |
Maleate |
T8205 |
1885-1889 |
NN |
denotes |
Tris |
T8206 |
1889-1890 |
HYPH |
denotes |
- |
T8207 |
1897-1899 |
, |
denotes |
, |
T8208 |
1899-1901 |
NN |
denotes |
pH |
T8209 |
1904-1907 |
CD |
denotes |
9.0 |
T8210 |
1902-1903 |
SYM |
denotes |
= |
T8211 |
1907-1909 |
, |
denotes |
, |
T8212 |
1909-1912 |
CD |
denotes |
0.8 |
T8213 |
1913-1915 |
NN |
denotes |
mM |
T8214 |
1916-1921 |
NN |
denotes |
MgCl2 |
T8215 |
1921-1923 |
, |
denotes |
, |
T8216 |
1923-1926 |
CD |
denotes |
0.4 |
T8217 |
1927-1929 |
NN |
denotes |
mg |
T8218 |
1948-1957 |
NN |
denotes |
phosphate |
T8219 |
1929-1930 |
SYM |
denotes |
/ |
T8220 |
1930-1932 |
NN |
denotes |
ml |
T8221 |
1933-1938 |
NN |
denotes |
alpha |
T8222 |
1939-1947 |
NN |
denotes |
naphthyl |
T8223 |
1938-1939 |
HYPH |
denotes |
- |
T8224 |
1957-1959 |
, |
denotes |
, |
T8225 |
1959-1960 |
CD |
denotes |
1 |
T8226 |
1963-1964 |
SYM |
denotes |
/ |
T8227 |
1964-1966 |
NN |
denotes |
ml |
T8228 |
1967-1971 |
JJ |
denotes |
Fast |
T8229 |
1971-1972 |
HYPH |
denotes |
- |
T8230 |
1972-1975 |
JJ |
denotes |
Red |
T8231 |
1975-1976 |
-RRB- |
denotes |
) |
T8232 |
1976-1977 |
. |
denotes |
. |
T8233 |
1977-2029 |
sentence |
denotes |
Stained embryos were washed in 1 × PBS/0.1% TX-100. |
T8234 |
1978-1985 |
VBN |
denotes |
Stained |
T8235 |
1986-1993 |
NNS |
denotes |
embryos |
T8236 |
1999-2005 |
VBN |
denotes |
washed |
T8237 |
1994-1998 |
VBD |
denotes |
were |
T8238 |
2006-2008 |
IN |
denotes |
in |
T8239 |
2009-2010 |
CD |
denotes |
1 |
T8240 |
2013-2016 |
NN |
denotes |
PBS |
T8241 |
2011-2012 |
SYM |
denotes |
× |
T8242 |
2016-2017 |
SYM |
denotes |
/ |
T8243 |
2022-2024 |
NN |
denotes |
TX |
T8244 |
2017-2020 |
CD |
denotes |
0.1 |
T8245 |
2020-2021 |
NN |
denotes |
% |
T8246 |
2024-2025 |
HYPH |
denotes |
- |
T8247 |
2025-2028 |
CD |
denotes |
100 |
T8248 |
2028-2029 |
. |
denotes |
. |
T8304 |
2221-2223 |
CD |
denotes |
56 |
T8305 |
2223-2224 |
-RRB- |
denotes |
] |
T8306 |
2224-2225 |
-RRB- |
denotes |
) |
T8307 |
2226-2229 |
IN |
denotes |
for |
T8308 |
2230-2232 |
CD |
denotes |
24 |
T8309 |
2233-2238 |
NNS |
denotes |
hours |
T8310 |
2239-2244 |
IN |
denotes |
while |
T8311 |
2245-2253 |
VBG |
denotes |
rotating |
T8312 |
2254-2256 |
IN |
denotes |
in |
T8313 |
2257-2258 |
DT |
denotes |
a |
T8314 |
2264-2273 |
NN |
denotes |
incubator |
T8315 |
2259-2261 |
CD |
denotes |
37 |
T8316 |
2261-2263 |
NN |
denotes |
°C |
T8317 |
2274-2276 |
IN |
denotes |
in |
T8318 |
2277-2278 |
CD |
denotes |
5 |
T8319 |
2278-2279 |
NN |
denotes |
% |
T8320 |
2280-2283 |
NN |
denotes |
CO2 |
T8321 |
2283-2284 |
. |
denotes |
. |
T8322 |
2284-2345 |
sentence |
denotes |
Embryos were then fixed in 4% paraformaldehyde for analysis. |
T8323 |
2285-2292 |
NNS |
denotes |
Embryos |
T8324 |
2303-2308 |
VBN |
denotes |
fixed |
T8325 |
2293-2297 |
VBD |
denotes |
were |
T8326 |
2298-2302 |
RB |
denotes |
then |
T8327 |
2309-2311 |
IN |
denotes |
in |
T8328 |
2312-2313 |
CD |
denotes |
4 |
T8329 |
2313-2314 |
NN |
denotes |
% |
T8330 |
2315-2331 |
NN |
denotes |
paraformaldehyde |
T8331 |
2332-2335 |
IN |
denotes |
for |
T8332 |
2336-2344 |
NN |
denotes |
analysis |
T8333 |
2344-2345 |
. |
denotes |
. |
T8266 |
2031-2036 |
JJ |
denotes |
Whole |
T8267 |
2037-2043 |
NN |
denotes |
embryo |
T8268 |
2044-2051 |
NN |
denotes |
culture |
T8269 |
2051-2102 |
sentence |
denotes |
Embryos were dissected at E7.5 and washed in DMEM. |
T8270 |
2052-2059 |
NNS |
denotes |
Embryos |
T8271 |
2065-2074 |
VBN |
denotes |
dissected |
T8272 |
2060-2064 |
VBD |
denotes |
were |
T8273 |
2075-2077 |
IN |
denotes |
at |
T8274 |
2078-2082 |
NN |
denotes |
E7.5 |
T8275 |
2083-2086 |
CC |
denotes |
and |
T8276 |
2087-2093 |
VBN |
denotes |
washed |
T8277 |
2094-2096 |
IN |
denotes |
in |
T8278 |
2097-2101 |
NN |
denotes |
DMEM |
T8279 |
2101-2102 |
. |
denotes |
. |
T8280 |
2102-2284 |
sentence |
denotes |
Embryos were then cultured individually in 1 ml of culture media (75% immediately centrifuged rat serum and 25% DMEM [56]) for 24 hours while rotating in a 37°C incubator in 5% CO2. |
T8281 |
2103-2110 |
NNS |
denotes |
Embryos |
T8282 |
2121-2129 |
VBN |
denotes |
cultured |
T8283 |
2111-2115 |
VBD |
denotes |
were |
T8284 |
2116-2120 |
RB |
denotes |
then |
T8285 |
2130-2142 |
RB |
denotes |
individually |
T8286 |
2143-2145 |
IN |
denotes |
in |
T8287 |
2146-2147 |
CD |
denotes |
1 |
T8288 |
2148-2150 |
NN |
denotes |
ml |
T8289 |
2151-2153 |
IN |
denotes |
of |
T8290 |
2154-2161 |
NN |
denotes |
culture |
T8291 |
2162-2167 |
NNS |
denotes |
media |
T8292 |
2168-2169 |
-LRB- |
denotes |
( |
T8293 |
2169-2171 |
CD |
denotes |
75 |
T8294 |
2171-2172 |
NN |
denotes |
% |
T8295 |
2201-2206 |
NN |
denotes |
serum |
T8296 |
2173-2184 |
RB |
denotes |
immediately |
T8297 |
2185-2196 |
VBN |
denotes |
centrifuged |
T8298 |
2197-2200 |
NN |
denotes |
rat |
T8299 |
2207-2210 |
CC |
denotes |
and |
T8300 |
2211-2213 |
CD |
denotes |
25 |
T8301 |
2213-2214 |
NN |
denotes |
% |
T8302 |
2215-2219 |
NN |
denotes |
DMEM |
T8303 |
2220-2221 |
-LRB- |
denotes |
[ |
T8126 |
1525-1533 |
NN |
denotes |
Alkaline |
R4667 |
T7726 |
T7725 |
cc |
and,Mice |
R4668 |
T7727 |
T7725 |
conj |
genotyping,Mice |
R4669 |
T7729 |
T7730 |
det |
The,mice |
R4670 |
T7730 |
T7734 |
nsubjpass |
mice,described |
R4671 |
T7731 |
T7730 |
nmod |
Hdhex4,mice |
R4672 |
T7732 |
T7731 |
punct |
/,Hdhex4 |
R4673 |
T7733 |
T7731 |
nummod |
5,Hdhex4 |
R4674 |
T7735 |
T7730 |
acl |
carrying,mice |
R4675 |
T7736 |
T7737 |
det |
a,mutation |
R4676 |
T7737 |
T7735 |
dobj |
mutation,carrying |
R4677 |
T7738 |
T7739 |
compound |
pGKneo,replacement |
R4678 |
T7739 |
T7742 |
npadvmod |
replacement,inactivating |
R4679 |
T7740 |
T7739 |
compound |
insertion,replacement |
R4680 |
T7741 |
T7739 |
punct |
/,replacement |
R4681 |
T7742 |
T7737 |
amod |
inactivating,mutation |
R4682 |
T7743 |
T7735 |
prep |
in,carrying |
R4683 |
T7744 |
T7745 |
det |
the,homologue |
R4684 |
T7745 |
T7743 |
pobj |
homologue,in |
R4685 |
T7746 |
T7745 |
compound |
mouse,homologue |
R4686 |
T7747 |
T7745 |
compound |
HD,homologue |
R4687 |
T7748 |
T7745 |
compound |
gene,homologue |
R4688 |
T7749 |
T7734 |
aux |
have,described |
R4689 |
T7750 |
T7734 |
auxpass |
been,described |
R4690 |
T7751 |
T7734 |
advmod |
previously,described |
R4691 |
T7752 |
T7753 |
punct |
[,10 |
R4692 |
T7753 |
T7734 |
parataxis |
10,described |
R4693 |
T7754 |
T7753 |
punct |
],10 |
R4694 |
T7755 |
T7734 |
punct |
.,described |
R4695 |
T7757 |
T7758 |
det |
The,experiments |
R4696 |
T7758 |
T7759 |
nsubjpass |
experiments,conducted |
R4697 |
T7760 |
T7759 |
auxpass |
were,conducted |
R4698 |
T7761 |
T7759 |
prep |
in,conducted |
R4699 |
T7762 |
T7761 |
pobj |
accordance,in |
R4700 |
T7763 |
T7762 |
prep |
with,accordance |
R4701 |
T7764 |
T7765 |
det |
an,protocol |
R4702 |
T7765 |
T7763 |
pobj |
protocol,with |
R4703 |
T7766 |
T7767 |
npadvmod |
IACUC,approved |
R4704 |
T7767 |
T7765 |
amod |
approved,protocol |
R4705 |
T7768 |
T7759 |
punct |
", ",conducted |
R4706 |
T7769 |
T7759 |
prep |
through,conducted |
R4707 |
T7770 |
T7771 |
det |
the,Subcommittee |
R4708 |
T7771 |
T7769 |
pobj |
Subcommittee,through |
R4709 |
T7772 |
T7771 |
compound |
MGH,Subcommittee |
R4710 |
T7773 |
T7771 |
prep |
on,Subcommittee |
R4711 |
T7774 |
T7775 |
compound |
Animal,Research |
R4712 |
T7775 |
T7773 |
pobj |
Research,on |
R4713 |
T7776 |
T7759 |
punct |
.,conducted |
R4714 |
T7778 |
T7779 |
nmod |
Mutant,Hdhex4 |
R4715 |
T7779 |
T7780 |
nmod |
Hdhex4,littermates |
R4716 |
T7780 |
T7789 |
nsubjpass |
littermates,obtained |
R4717 |
T7781 |
T7779 |
punct |
/,Hdhex4 |
R4718 |
T7782 |
T7779 |
nummod |
5,Hdhex4 |
R4719 |
T7783 |
T7779 |
punct |
/,Hdhex4 |
R4720 |
T7784 |
T7779 |
appos |
Hdhex4,Hdhex4 |
R4721 |
T7785 |
T7784 |
punct |
/,Hdhex4 |
R4722 |
T7786 |
T7784 |
nummod |
5,Hdhex4 |
R4723 |
T7787 |
T7779 |
cc |
and,Hdhex4 |
R4724 |
T7788 |
T7779 |
conj |
normal,Hdhex4 |
R4725 |
T7790 |
T7789 |
auxpass |
were,obtained |
R4726 |
T7791 |
T7789 |
prep |
in,obtained |
R4727 |
T7792 |
T7793 |
amod |
timed,pregnancies |
R4728 |
T7793 |
T7791 |
pobj |
pregnancies,in |
R4729 |
T7794 |
T7789 |
prep |
from,obtained |
R4730 |
T7795 |
T7794 |
pobj |
mating,from |
R4731 |
T7796 |
T7795 |
prep |
of,mating |
R4732 |
T7797 |
T7798 |
nmod |
Hdhex4,heterozygotes |
R4733 |
T7798 |
T7796 |
pobj |
heterozygotes,of |
R4734 |
T7799 |
T7797 |
punct |
/,Hdhex4 |
R4735 |
T7800 |
T7797 |
nummod |
5,Hdhex4 |
R4736 |
T7801 |
T7797 |
punct |
/,Hdhex4 |
R4737 |
T7802 |
T7797 |
appos |
Hdh,Hdhex4 |
R4738 |
T7803 |
T7802 |
punct |
+,Hdh |
R4739 |
T7804 |
T7798 |
punct |
", ",heterozygotes |
R4740 |
T7805 |
T7798 |
acl |
genotyped,heterozygotes |
R4741 |
T7806 |
T7805 |
prep |
by,genotyped |
R4742 |
T7807 |
T7808 |
compound |
PCR,assay |
R4743 |
T7808 |
T7806 |
pobj |
assay,by |
R4744 |
T7809 |
T7789 |
punct |
", ",obtained |
R4745 |
T7810 |
T7811 |
mark |
as,described |
R4746 |
T7811 |
T7789 |
advcl |
described,obtained |
R4747 |
T7812 |
T7813 |
punct |
[,10 |
R4748 |
T7813 |
T7811 |
parataxis |
10,described |
R4749 |
T7814 |
T7813 |
punct |
],10 |
R4750 |
T7815 |
T7789 |
punct |
.,obtained |
R4751 |
T7817 |
T7818 |
det |
The,day |
R4752 |
T7818 |
T7819 |
nsubjpass |
day,taken |
R4753 |
T7820 |
T7818 |
prep |
of,day |
R4754 |
T7821 |
T7820 |
pobj |
plug,of |
R4755 |
T7822 |
T7819 |
auxpass |
was,taken |
R4756 |
T7823 |
T7824 |
aux |
to,be |
R4757 |
T7824 |
T7819 |
xcomp |
be,taken |
R4758 |
T7825 |
T7824 |
attr |
E0.5,be |
R4759 |
T7826 |
T7819 |
punct |
.,taken |
R4760 |
T7828 |
T7829 |
nsubjpass |
Embryos,pooled |
R4761 |
T7830 |
T7831 |
dep |
that,were |
R4762 |
T7831 |
T7828 |
relcl |
were,Embryos |
R4763 |
T7832 |
T7833 |
advmod |
morphologically,normal |
R4764 |
T7833 |
T7831 |
acomp |
normal,were |
R4765 |
T7834 |
T7829 |
auxpass |
were,pooled |
R4766 |
T7835 |
T7836 |
advmod |
separately,from |
R4767 |
T7836 |
T7829 |
prep |
from,pooled |
R4768 |
T7837 |
T7838 |
advmod |
morphologically,mutant |
R4769 |
T7838 |
T7839 |
compound |
mutant,embryos |
R4770 |
T7839 |
T7836 |
pobj |
embryos,from |
R4771 |
T7840 |
T7829 |
prep |
for,pooled |
R4772 |
T7841 |
T7840 |
pobj |
analysis,for |
R4773 |
T7842 |
T7829 |
punct |
.,pooled |
R4774 |
T7844 |
T7845 |
compound |
Nodal,expression |
R4775 |
T7845 |
T7846 |
nsubjpass |
expression,determined |
R4776 |
T7847 |
T7846 |
auxpass |
was,determined |
R4777 |
T7848 |
T7846 |
prep |
in,determined |
R4778 |
T7849 |
T7848 |
pobj |
embryos,in |
R4779 |
T7850 |
T7849 |
prep |
from,embryos |
R4780 |
T7851 |
T7850 |
pobj |
matings,from |
R4781 |
T7852 |
T7851 |
prep |
of,matings |
R4782 |
T7853 |
T7854 |
nmod |
Hdhex4,heterozygotes |
R4783 |
T7854 |
T7852 |
pobj |
heterozygotes,of |
R4784 |
T7855 |
T7853 |
punct |
/,Hdhex4 |
R4785 |
T7856 |
T7853 |
nummod |
5,Hdhex4 |
R4786 |
T7857 |
T7853 |
punct |
/,Hdhex4 |
R4787 |
T7858 |
T7853 |
appos |
Hdh,Hdhex4 |
R4788 |
T7859 |
T7858 |
punct |
+,Hdh |
R4789 |
T7860 |
T7853 |
punct |
;,Hdhex4 |
R4790 |
T7861 |
T7862 |
compound |
NdllacZ,Ndl |
R4791 |
T7862 |
T7853 |
appos |
Ndl,Hdhex4 |
R4792 |
T7863 |
T7862 |
punct |
/,Ndl |
R4793 |
T7864 |
T7862 |
punct |
+,Ndl |
R4794 |
T7865 |
T7854 |
compound |
compound,heterozygotes |
R4795 |
T7866 |
T7854 |
acl |
genotyped,heterozygotes |
R4796 |
T7867 |
T7866 |
prep |
by,genotyped |
R4797 |
T7868 |
T7869 |
compound |
PCR,assay |
R4798 |
T7869 |
T7867 |
pobj |
assay,by |
R4799 |
T7870 |
T7871 |
mark |
as,described |
R4800 |
T7871 |
T7866 |
advcl |
described,genotyped |
R4801 |
T7872 |
T7871 |
prep |
in,described |
R4802 |
T7873 |
T7874 |
punct |
[,29 |
R4803 |
T7874 |
T7872 |
pobj |
29,in |
R4804 |
T7875 |
T7874 |
punct |
],29 |
R4805 |
T7876 |
T7846 |
punct |
.,determined |
R4806 |
T7923 |
T7924 |
amod |
Whole,mount |
R4807 |
T7924 |
T7925 |
nmod |
mount,hybridization |
R4808 |
T7926 |
T7924 |
cc |
and,mount |
R4809 |
T7927 |
T7924 |
conj |
section,mount |
R4810 |
T7928 |
T7929 |
advmod |
in,situ |
R4811 |
T7929 |
T7925 |
amod |
situ,hybridization |
R4812 |
T7930 |
T7925 |
cc |
and,hybridization |
R4813 |
T7931 |
T7932 |
compound |
β,gal |
R4814 |
T7932 |
T7934 |
compound |
gal,staining |
R4815 |
T7933 |
T7932 |
punct |
-,gal |
R4816 |
T7934 |
T7925 |
conj |
staining,hybridization |
R4817 |
T7936 |
T7937 |
prep |
After,fixed |
R4818 |
T7938 |
T7936 |
pobj |
dissection,After |
R4819 |
T7939 |
T7938 |
prep |
in,dissection |
R4820 |
T7940 |
T7939 |
pobj |
PBS,in |
R4821 |
T7941 |
T7937 |
punct |
", ",fixed |
R4822 |
T7942 |
T7937 |
nsubjpass |
embryos,fixed |
R4823 |
T7943 |
T7937 |
auxpass |
were,fixed |
R4824 |
T7944 |
T7937 |
advmod |
overnight,fixed |
R4825 |
T7945 |
T7937 |
prep |
in,fixed |
R4826 |
T7946 |
T7947 |
nummod |
4,% |
R4827 |
T7947 |
T7948 |
compound |
%,paraformaldehyde |
R4828 |
T7948 |
T7945 |
pobj |
paraformaldehyde,in |
R4829 |
T7949 |
T7937 |
prep |
at,fixed |
R4830 |
T7950 |
T7951 |
nummod |
4,°C |
R4831 |
T7951 |
T7949 |
pobj |
°C,at |
R4832 |
T7952 |
T7937 |
punct |
.,fixed |
R4833 |
T7954 |
T7955 |
prep |
For,embedded |
R4834 |
T7956 |
T7954 |
pobj |
sections,For |
R4835 |
T7957 |
T7955 |
punct |
", ",embedded |
R4836 |
T7958 |
T7955 |
nsubjpass |
decidua,embedded |
R4837 |
T7959 |
T7958 |
acl |
fixed,decidua |
R4838 |
T7960 |
T7959 |
prep |
in,fixed |
R4839 |
T7961 |
T7962 |
nummod |
4,% |
R4840 |
T7962 |
T7963 |
compound |
%,paraformaldehyde |
R4841 |
T7963 |
T7960 |
pobj |
paraformaldehyde,in |
R4842 |
T7964 |
T7955 |
punct |
", ",embedded |
R4843 |
T7965 |
T7955 |
auxpass |
were,embedded |
R4844 |
T7966 |
T7955 |
prep |
in,embedded |
R4845 |
T7967 |
T7966 |
pobj |
paraffin,in |
R4846 |
T7968 |
T7955 |
cc |
and,embedded |
R4847 |
T7969 |
T7955 |
conj |
sectioned,embedded |
R4848 |
T7970 |
T7969 |
prep |
at,sectioned |
R4849 |
T7971 |
T7972 |
nummod |
7,microns |
R4850 |
T7972 |
T7970 |
pobj |
microns,at |
R4851 |
T7973 |
T7955 |
punct |
.,embedded |
R4852 |
T7975 |
T7976 |
nmod |
RNA,hybridizations |
R4853 |
T7976 |
T7979 |
nsubjpass |
hybridizations,performed |
R4854 |
T7977 |
T7978 |
advmod |
in,situ |
R4855 |
T7978 |
T7976 |
amod |
situ,hybridizations |
R4856 |
T7980 |
T7979 |
auxpass |
were,performed |
R4857 |
T7981 |
T7982 |
mark |
as,described |
R4858 |
T7982 |
T7979 |
advcl |
described,performed |
R4859 |
T7983 |
T7982 |
advmod |
previously,described |
R4860 |
T7984 |
T7985 |
punct |
[,55 |
R4861 |
T7985 |
T7982 |
parataxis |
55,described |
R4862 |
T7986 |
T7985 |
punct |
],55 |
R4863 |
T7987 |
T7979 |
punct |
.,performed |
R4864 |
T7989 |
T7990 |
compound |
Nodal,lacZ |
R4865 |
T7990 |
T7992 |
compound |
lacZ,expression |
R4866 |
T7991 |
T7990 |
punct |
.,lacZ |
R4867 |
T7992 |
T7993 |
nsubjpass |
expression,assessed |
R4868 |
T7994 |
T7993 |
auxpass |
was,assessed |
R4869 |
T7995 |
T7993 |
prep |
by,assessed |
R4870 |
T7996 |
T7997 |
compound |
β,galactosidase |
R4871 |
T7997 |
T7999 |
compound |
galactosidase,staining |
R4872 |
T7998 |
T7997 |
punct |
-,galactosidase |
R4873 |
T7999 |
T7995 |
pobj |
staining,by |
R4874 |
T8000 |
T8001 |
mark |
as,reported |
R4875 |
T8001 |
T7993 |
advcl |
reported,assessed |
R4876 |
T8002 |
T8003 |
punct |
[,29 |
R4877 |
T8003 |
T8001 |
parataxis |
29,reported |
R4878 |
T8004 |
T8003 |
punct |
],29 |
R4879 |
T8005 |
T7993 |
punct |
", ",assessed |
R4880 |
T8006 |
T7993 |
prep |
on,assessed |
R4881 |
T8007 |
T8006 |
pobj |
embryos,on |
R4882 |
T8008 |
T8009 |
dep |
post,fixed |
R4883 |
T8009 |
T8007 |
acl |
fixed,embryos |
R4884 |
T8010 |
T8009 |
prep |
in,fixed |
R4885 |
T8011 |
T8012 |
nummod |
4,% |
R4886 |
T8012 |
T8013 |
compound |
%,paraformaldehyde |
R4887 |
T8013 |
T8010 |
pobj |
paraformaldehyde,in |
R4888 |
T8014 |
T7993 |
punct |
.,assessed |
R4889 |
T8016 |
T8017 |
nsubjpass |
Embryos,mounted |
R4890 |
T8018 |
T8017 |
auxpass |
were,mounted |
R4891 |
T8019 |
T8017 |
prep |
in,mounted |
R4892 |
T8020 |
T8021 |
nummod |
80,% |
R4893 |
T8021 |
T8022 |
compound |
%,glycerol |
R4894 |
T8022 |
T8019 |
pobj |
glycerol,in |
R4895 |
T8023 |
T8017 |
prep |
before,mounted |
R4896 |
T8024 |
T8025 |
auxpass |
being,photographed |
R4897 |
T8025 |
T8023 |
pcomp |
photographed,before |
R4898 |
T8026 |
T8017 |
punct |
.,mounted |
R4899 |
T8028 |
T8029 |
det |
The,phenotype |
R4900 |
T8029 |
T8032 |
nsubj |
phenotype,is |
R4901 |
T8030 |
T8031 |
npadvmod |
huntingtin,deficient |
R4902 |
T8031 |
T8029 |
amod |
deficient,phenotype |
R4903 |
T8033 |
T8034 |
advmod |
fully,penetrant |
R4904 |
T8034 |
T8032 |
acomp |
penetrant,is |
R4905 |
T8035 |
T8032 |
prep |
at,is |
R4906 |
T8036 |
T8035 |
pobj |
each,at |
R4907 |
T8037 |
T8036 |
prep |
of,each |
R4908 |
T8038 |
T8039 |
det |
the,stages |
R4909 |
T8039 |
T8037 |
pobj |
stages,of |
R4910 |
T8040 |
T8041 |
dep |
that,assessed |
R4911 |
T8041 |
T8039 |
relcl |
assessed,stages |
R4912 |
T8042 |
T8041 |
auxpass |
were,assessed |
R4913 |
T8043 |
T8044 |
punct |
[,10 |
R4914 |
T8044 |
T8041 |
parataxis |
10,assessed |
R4915 |
T8045 |
T8044 |
punct |
],10 |
R4916 |
T8046 |
T8032 |
punct |
.,is |
R4917 |
T8048 |
T8049 |
quantmod |
Three,six |
R4918 |
T8049 |
T8051 |
nummod |
six,embryos |
R4919 |
T8050 |
T8049 |
quantmod |
to,six |
R4920 |
T8051 |
T8052 |
nsubjpass |
embryos,evaluated |
R4921 |
T8053 |
T8052 |
auxpass |
were,evaluated |
R4922 |
T8054 |
T8052 |
prep |
for,evaluated |
R4923 |
T8055 |
T8056 |
det |
each,marker |
R4924 |
T8056 |
T8054 |
pobj |
marker,for |
R4925 |
T8057 |
T8052 |
punct |
", ",evaluated |
R4926 |
T8058 |
T8052 |
prep |
with,evaluated |
R4927 |
T8059 |
T8060 |
det |
every,embryo |
R4928 |
T8060 |
T8061 |
nsubj |
embryo,exhibiting |
R4929 |
T8061 |
T8058 |
pcomp |
exhibiting,with |
R4930 |
T8062 |
T8063 |
det |
the,phenotype |
R4931 |
T8063 |
T8061 |
dobj |
phenotype,exhibiting |
R4932 |
T8064 |
T8063 |
amod |
same,phenotype |
R4933 |
T8065 |
T8063 |
compound |
mutant,phenotype |
R4934 |
T8066 |
T8061 |
prep |
in,exhibiting |
R4935 |
T8067 |
T8068 |
det |
each,case |
R4936 |
T8068 |
T8066 |
pobj |
case,in |
R4937 |
T8069 |
T8052 |
punct |
.,evaluated |
R4942 |
T8131 |
T8129 |
pobj |
Cells,of |
R4943 |
T8132 |
T8131 |
compound |
Germ,Cells |
R4944 |
T8133 |
T8131 |
punct |
(,Cells |
R4945 |
T8134 |
T8131 |
appos |
PCGs,Cells |
R4946 |
T8135 |
T8131 |
punct |
),Cells |
R4947 |
T8137 |
T8138 |
prep |
After,fixed |
R4948 |
T8139 |
T8137 |
pobj |
dissections,After |
R4949 |
T8140 |
T8138 |
punct |
", ",fixed |
R4950 |
T8141 |
T8138 |
nsubjpass |
embryos,fixed |
R4951 |
T8142 |
T8138 |
auxpass |
were,fixed |
R4952 |
T8143 |
T8138 |
prep |
in,fixed |
R4953 |
T8144 |
T8145 |
nummod |
4,% |
R4954 |
T8145 |
T8146 |
compound |
%,paraformaldehyde |
R4955 |
T8146 |
T8143 |
pobj |
paraformaldehyde,in |
R4956 |
T8147 |
T8138 |
advmod |
briefly,fixed |
R4957 |
T8148 |
T8138 |
cc |
and,fixed |
R4958 |
T8149 |
T8138 |
conj |
washed,fixed |
R4959 |
T8150 |
T8149 |
cc |
and,washed |
R4960 |
T8151 |
T8149 |
conj |
stored,washed |
R4961 |
T8152 |
T8151 |
prep |
in,stored |
R4962 |
T8153 |
T8154 |
nummod |
1,PBS |
R4963 |
T8154 |
T8152 |
pobj |
PBS,in |
R4964 |
T8155 |
T8153 |
punct |
×,1 |
R4965 |
T8156 |
T8154 |
punct |
/,PBS |
R4966 |
T8157 |
T8158 |
compound |
0.1,% |
R4967 |
T8158 |
T8159 |
compound |
%,TX |
R4968 |
T8159 |
T8154 |
appos |
TX,PBS |
R4969 |
T8160 |
T8159 |
punct |
-,TX |
R4970 |
T8161 |
T8159 |
nummod |
100,TX |
R4971 |
T8162 |
T8151 |
prep |
at,stored |
R4972 |
T8163 |
T8164 |
nummod |
4,°C |
R4973 |
T8164 |
T8162 |
pobj |
°C,at |
R4974 |
T8165 |
T8138 |
punct |
.,fixed |
R4975 |
T8167 |
T8168 |
nsubjpass |
Embryos,washed |
R4976 |
T8169 |
T8168 |
auxpass |
were,washed |
R4977 |
T8170 |
T8168 |
advmod |
once,washed |
R4978 |
T8171 |
T8168 |
prep |
with,washed |
R4979 |
T8172 |
T8173 |
compound |
Tris,Maleate |
R4980 |
T8173 |
T8175 |
compound |
Maleate,Buffer |
R4981 |
T8174 |
T8173 |
punct |
-,Maleate |
R4982 |
T8175 |
T8171 |
pobj |
Buffer,with |
R4983 |
T8176 |
T8177 |
punct |
(,MgCl2 |
R4984 |
T8177 |
T8175 |
parataxis |
MgCl2,Buffer |
R4985 |
T8178 |
T8179 |
nummod |
25,mM |
R4986 |
T8179 |
T8180 |
compound |
mM,Maleate |
R4987 |
T8180 |
T8177 |
dep |
Maleate,MgCl2 |
R4988 |
T8181 |
T8180 |
compound |
Tris,Maleate |
R4989 |
T8182 |
T8180 |
punct |
-,Maleate |
R4990 |
T8183 |
T8177 |
punct |
", ",MgCl2 |
R4991 |
T8184 |
T8185 |
nsubj |
pH,9.0 |
R4992 |
T8185 |
T8177 |
ccomp |
9.0,MgCl2 |
R4993 |
T8186 |
T8185 |
punct |
=,9.0 |
R4994 |
T8187 |
T8177 |
punct |
", ",MgCl2 |
R4995 |
T8188 |
T8189 |
nummod |
0.8,mM |
R4996 |
T8189 |
T8177 |
compound |
mM,MgCl2 |
R4997 |
T8190 |
T8177 |
punct |
),MgCl2 |
R4998 |
T8191 |
T8168 |
cc |
and,washed |
R4999 |
T8192 |
T8193 |
auxpass |
were,incubated |
R5000 |
T8193 |
T8168 |
conj |
incubated,washed |
R5001 |
T8194 |
T8193 |
advmod |
subsequently,incubated |
R5002 |
T8195 |
T8193 |
prep |
in,incubated |
R5003 |
T8196 |
T8197 |
compound |
alkaline,phosphatase |
R5004 |
T8197 |
T8198 |
compound |
phosphatase,solution |
R5005 |
T8198 |
T8195 |
pobj |
solution,in |
R5006 |
T8199 |
T8198 |
compound |
staining,solution |
R5007 |
T8200 |
T8201 |
punct |
(,mg |
R5008 |
T8201 |
T8193 |
parataxis |
mg,incubated |
R5009 |
T8202 |
T8203 |
nummod |
25,mM |
R5010 |
T8203 |
T8204 |
compound |
mM,Maleate |
R5011 |
T8204 |
T8201 |
dep |
Maleate,mg |
R5012 |
T8205 |
T8204 |
compound |
Tris,Maleate |
R5013 |
T8206 |
T8204 |
punct |
-,Maleate |
R5014 |
T8207 |
T8201 |
punct |
", ",mg |
R5015 |
T8208 |
T8209 |
nsubj |
pH,9.0 |
R5016 |
T8209 |
T8201 |
ccomp |
9.0,mg |
R5017 |
T8210 |
T8209 |
punct |
=,9.0 |
R5018 |
T8211 |
T8201 |
punct |
", ",mg |
R5019 |
T8212 |
T8213 |
nummod |
0.8,mM |
R5020 |
T8213 |
T8214 |
compound |
mM,MgCl2 |
R5021 |
T8214 |
T8201 |
dep |
MgCl2,mg |
R5022 |
T8215 |
T8201 |
punct |
", ",mg |
R5023 |
T8216 |
T8217 |
nummod |
0.4,mg |
R5024 |
T8217 |
T8218 |
nmod |
mg,phosphate |
R5025 |
T8218 |
T8201 |
dep |
phosphate,mg |
R5026 |
T8219 |
T8220 |
punct |
/,ml |
R5027 |
T8220 |
T8217 |
prep |
ml,mg |
R5028 |
T8221 |
T8222 |
compound |
alpha,naphthyl |
R5029 |
T8222 |
T8218 |
compound |
naphthyl,phosphate |
R5030 |
T8223 |
T8222 |
punct |
-,naphthyl |
R5031 |
T8224 |
T8201 |
punct |
", ",mg |
R5032 |
T8225 |
T8201 |
nummod |
1,mg |
R5033 |
T8226 |
T8227 |
punct |
/,ml |
R5034 |
T8227 |
T8201 |
prep |
ml,mg |
R5035 |
T8228 |
T8201 |
amod |
Fast,mg |
R5036 |
T8229 |
T8201 |
punct |
-,mg |
R5037 |
T8230 |
T8201 |
amod |
Red,mg |
R5038 |
T8231 |
T8201 |
punct |
),mg |
R5039 |
T8232 |
T8168 |
punct |
.,washed |
R5040 |
T8234 |
T8235 |
amod |
Stained,embryos |
R5041 |
T8235 |
T8236 |
nsubjpass |
embryos,washed |
R5042 |
T8237 |
T8236 |
auxpass |
were,washed |
R5043 |
T8238 |
T8236 |
prep |
in,washed |
R5044 |
T8239 |
T8240 |
nummod |
1,PBS |
R5045 |
T8240 |
T8238 |
pobj |
PBS,in |
R5046 |
T8241 |
T8239 |
punct |
×,1 |
R5047 |
T8242 |
T8243 |
punct |
/,TX |
R5048 |
T8243 |
T8240 |
prep |
TX,PBS |
R5049 |
T8244 |
T8245 |
nummod |
0.1,% |
R5050 |
T8245 |
T8243 |
compound |
%,TX |
R5051 |
T8246 |
T8243 |
punct |
-,TX |
R5052 |
T8247 |
T8243 |
nummod |
100,TX |
R5053 |
T8248 |
T8236 |
punct |
.,washed |
R5054 |
T8266 |
T8267 |
amod |
Whole,embryo |
R5055 |
T8267 |
T8268 |
compound |
embryo,culture |
R5056 |
T8270 |
T8271 |
nsubjpass |
Embryos,dissected |
R5057 |
T8272 |
T8271 |
auxpass |
were,dissected |
R5058 |
T8273 |
T8271 |
prep |
at,dissected |
R5059 |
T8274 |
T8273 |
pobj |
E7.5,at |
R5060 |
T8275 |
T8271 |
cc |
and,dissected |
R5061 |
T8276 |
T8271 |
conj |
washed,dissected |
R5062 |
T8277 |
T8276 |
prep |
in,washed |
R5063 |
T8278 |
T8277 |
pobj |
DMEM,in |
R5064 |
T8279 |
T8271 |
punct |
.,dissected |
R5065 |
T8281 |
T8282 |
nsubjpass |
Embryos,cultured |
R5066 |
T8283 |
T8282 |
auxpass |
were,cultured |
R5067 |
T8284 |
T8282 |
advmod |
then,cultured |
R5068 |
T8285 |
T8282 |
advmod |
individually,cultured |
R5069 |
T8286 |
T8282 |
prep |
in,cultured |
R5070 |
T8287 |
T8288 |
nummod |
1,ml |
R5071 |
T8288 |
T8286 |
pobj |
ml,in |
R5072 |
T8289 |
T8288 |
prep |
of,ml |
R5073 |
T8290 |
T8291 |
compound |
culture,media |
R5074 |
T8291 |
T8289 |
pobj |
media,of |
R5075 |
T8292 |
T8291 |
punct |
(,media |
R5076 |
T8293 |
T8294 |
nummod |
75,% |
R5077 |
T8294 |
T8295 |
nmod |
%,serum |
R5078 |
T8295 |
T8291 |
appos |
serum,media |
R5079 |
T8296 |
T8297 |
advmod |
immediately,centrifuged |
R5080 |
T8297 |
T8295 |
amod |
centrifuged,serum |
R5081 |
T8298 |
T8295 |
compound |
rat,serum |
R5082 |
T8299 |
T8295 |
cc |
and,serum |
R5083 |
T8300 |
T8301 |
nummod |
25,% |
R5084 |
T8301 |
T8302 |
compound |
%,DMEM |
R5085 |
T8302 |
T8295 |
conj |
DMEM,serum |
R5086 |
T8303 |
T8304 |
punct |
[,56 |
R5087 |
T8304 |
T8291 |
parataxis |
56,media |
R5088 |
T8305 |
T8304 |
punct |
],56 |
R5089 |
T8306 |
T8291 |
punct |
),media |
R5090 |
T8307 |
T8282 |
prep |
for,cultured |
R5091 |
T8308 |
T8309 |
nummod |
24,hours |
R5092 |
T8309 |
T8307 |
pobj |
hours,for |
R5093 |
T8310 |
T8311 |
prep |
while,rotating |
R5094 |
T8311 |
T8282 |
advcl |
rotating,cultured |
R5095 |
T8312 |
T8311 |
prep |
in,rotating |
R5096 |
T8313 |
T8314 |
det |
a,incubator |
R5097 |
T8314 |
T8312 |
pobj |
incubator,in |
R5098 |
T8315 |
T8316 |
nummod |
37,°C |
R5099 |
T8316 |
T8314 |
compound |
°C,incubator |
R5100 |
T8317 |
T8311 |
prep |
in,rotating |
R5101 |
T8318 |
T8319 |
nummod |
5,% |
R5102 |
T8319 |
T8320 |
compound |
%,CO2 |
R5103 |
T8320 |
T8317 |
pobj |
CO2,in |
R5104 |
T8321 |
T8282 |
punct |
.,cultured |
R5105 |
T8323 |
T8324 |
nsubjpass |
Embryos,fixed |
R5106 |
T8325 |
T8324 |
auxpass |
were,fixed |
R5107 |
T8326 |
T8324 |
advmod |
then,fixed |
R5108 |
T8327 |
T8324 |
prep |
in,fixed |
R5109 |
T8328 |
T8329 |
nummod |
4,% |
R5110 |
T8329 |
T8330 |
compound |
%,paraformaldehyde |
R5111 |
T8330 |
T8327 |
pobj |
paraformaldehyde,in |
R5112 |
T8331 |
T8324 |
prep |
for,fixed |
R5113 |
T8332 |
T8331 |
pobj |
analysis,for |
R5114 |
T8333 |
T8324 |
punct |
.,fixed |
R4938 |
T8126 |
T8127 |
compound |
Alkaline,phosphatase |
R4939 |
T8127 |
T8128 |
compound |
phosphatase,staining |
R4940 |
T8129 |
T8128 |
prep |
of,staining |
R4941 |
T8130 |
T8131 |
amod |
Primordial,Cells |