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Id Subject Object Predicate Lexical cue
T964 0-4 NN denotes Gene
T965 5-14 NN denotes Targeting
T966 15-18 CC denotes and
T967 19-29 NN denotes Generation
T968 30-32 IN denotes of
T969 33-37 NN denotes MCAD
T970 38-47 JJ denotes Deficient
T971 37-38 HYPH denotes -
T972 48-52 NNS denotes Mice
T973 52-219 sentence denotes MCAD insertion vector (MCAD IV2) was designed to undergo gap repair of the 1.3-kb deleted region upon homologous recombination in 129P2 (129P2/OlaHsd) ES cells E14–1.
T974 53-57 NN denotes MCAD
T975 68-74 NN denotes vector
T976 58-67 NN denotes insertion
T977 90-98 VBN denotes designed
T978 75-76 -LRB- denotes (
T979 76-80 NN denotes MCAD
T980 81-84 NN denotes IV2
T981 84-85 -RRB- denotes )
T982 86-89 VBD denotes was
T983 99-101 TO denotes to
T984 102-109 VB denotes undergo
T985 110-113 NN denotes gap
T986 114-120 NN denotes repair
T987 121-123 IN denotes of
T988 124-127 DT denotes the
T989 143-149 NN denotes region
T990 128-131 CD denotes 1.3
T991 132-134 NN denotes kb
T992 131-132 HYPH denotes -
T993 135-142 VBN denotes deleted
T994 150-154 IN denotes upon
T995 155-165 JJ denotes homologous
T996 166-179 NN denotes recombination
T997 180-182 IN denotes in
T998 183-188 NN denotes 129P2
T999 207-212 NNS denotes cells
T1000 189-190 -LRB- denotes (
T1001 196-202 NN denotes OlaHsd
T1002 190-195 NN denotes 129P2
T1003 195-196 HYPH denotes /
T1004 202-203 -RRB- denotes )
T1005 204-206 NN denotes ES
T1006 213-216 NN denotes E14
T1007 216-217 HYPH denotes –
T1008 217-218 CD denotes 1
T1009 218-219 . denotes .
T1010 219-370 sentence denotes Correct targeting of the MCAD locus resulted in a duplication of exons 8, 9, and 10 and integration of flanking plasmid and Neo sequences (Figure 1A).
T1011 220-227 JJ denotes Correct
T1012 228-237 NN denotes targeting
T1013 256-264 VBD denotes resulted
T1014 238-240 IN denotes of
T1015 241-244 DT denotes the
T1016 250-255 NN denotes locus
T1017 245-249 NN denotes MCAD
T1018 265-267 IN denotes in
T1019 268-269 DT denotes a
T1020 270-281 NN denotes duplication
T1021 282-284 IN denotes of
T1022 285-290 NNS denotes exons
T1023 291-292 CD denotes 8
T1024 292-294 , denotes ,
T1025 294-295 CD denotes 9
T1026 295-297 , denotes ,
T1027 297-300 CC denotes and
T1028 301-303 CD denotes 10
T1029 304-307 CC denotes and
T1030 308-319 NN denotes integration
T1031 320-322 IN denotes of
T1032 323-331 VBG denotes flanking
T1033 332-339 NN denotes plasmid
T1034 340-343 CC denotes and
T1035 344-347 NN denotes Neo
T1036 348-357 NNS denotes sequences
T1037 358-359 -LRB- denotes (
T1038 359-365 NN denotes Figure
T1039 366-368 CD denotes 1A
T1040 368-369 -RRB- denotes )
T1041 369-370 . denotes .
T1042 370-454 sentence denotes The insertion vector was designed to duplicate exon 8, 9, and 10 at the MCAD locus.
T1043 371-374 DT denotes The
T1044 385-391 NN denotes vector
T1045 375-384 NN denotes insertion
T1046 396-404 VBN denotes designed
T1047 392-395 VBD denotes was
T1048 405-407 TO denotes to
T1049 408-417 VB denotes duplicate
T1050 418-422 NN denotes exon
T1051 423-424 CD denotes 8
T1052 424-426 , denotes ,
T1053 426-427 CD denotes 9
T1054 427-429 , denotes ,
T1055 429-432 CC denotes and
T1056 433-435 CD denotes 10
T1057 436-438 IN denotes at
T1058 439-442 DT denotes the
T1059 448-453 NN denotes locus
T1060 443-447 NN denotes MCAD
T1061 453-454 . denotes .
T1062 454-593 sentence denotes Translation of the duplicated exon 8 region results in the formation of premature stop codons resulting in truncation of the MCAD monomer.
T1063 455-466 NN denotes Translation
T1064 499-506 VBZ denotes results
T1065 467-469 IN denotes of
T1066 470-473 DT denotes the
T1067 492-498 NN denotes region
T1068 474-484 VBN denotes duplicated
T1069 485-489 NN denotes exon
T1070 490-491 CD denotes 8
T1071 507-509 IN denotes in
T1072 510-513 DT denotes the
T1073 514-523 NN denotes formation
T1074 524-526 IN denotes of
T1075 527-536 JJ denotes premature
T1076 542-548 NNS denotes codons
T1077 537-541 NN denotes stop
T1078 549-558 VBG denotes resulting
T1079 559-561 IN denotes in
T1080 562-572 NN denotes truncation
T1081 573-575 IN denotes of
T1082 576-579 DT denotes the
T1083 585-592 NN denotes monomer
T1084 580-584 NN denotes MCAD
T1085 592-593 . denotes .
T1086 593-717 sentence denotes Specifically, the first premature stop codon arises after translation of only seven amino acids from the duplicated exon 8.
T1087 594-606 RB denotes Specifically
T1088 639-645 VBZ denotes arises
T1089 606-608 , denotes ,
T1090 608-611 DT denotes the
T1091 633-638 NN denotes codon
T1092 612-617 JJ denotes first
T1093 618-627 JJ denotes premature
T1094 628-632 NN denotes stop
T1095 646-651 IN denotes after
T1096 652-663 NN denotes translation
T1097 664-666 IN denotes of
T1098 667-671 RB denotes only
T1099 672-677 CD denotes seven
T1100 684-689 NNS denotes acids
T1101 678-683 NN denotes amino
T1102 690-694 IN denotes from
T1103 695-698 DT denotes the
T1104 710-714 NN denotes exon
T1105 699-709 VBN denotes duplicated
T1106 715-716 CD denotes 8
T1107 716-717 . denotes .
T1108 717-887 sentence denotes The resulting MCAD monomer is missing the C-terminal domain α-helixes that are responsible for making intersubunit contacts to generate the functional MCAD homotetramer.
T1109 718-721 DT denotes The
T1110 737-744 NN denotes monomer
T1111 722-731 VBG denotes resulting
T1112 732-736 NN denotes MCAD
T1113 748-755 VBG denotes missing
T1114 745-747 VBZ denotes is
T1115 756-759 DT denotes the
T1116 780-787 NNS denotes helixes
T1117 760-761 NN denotes C
T1118 762-770 JJ denotes terminal
T1119 761-762 HYPH denotes -
T1120 771-777 NN denotes domain
T1121 778-779 NN denotes α
T1122 779-780 HYPH denotes -
T1123 788-792 WDT denotes that
T1124 793-796 VBP denotes are
T1125 797-808 JJ denotes responsible
T1126 809-812 IN denotes for
T1127 813-819 VBG denotes making
T1128 820-832 NN denotes intersubunit
T1129 833-841 NNS denotes contacts
T1130 842-844 TO denotes to
T1131 845-853 VB denotes generate
T1132 854-857 DT denotes the
T1133 874-886 NN denotes homotetramer
T1134 858-868 JJ denotes functional
T1135 869-873 NN denotes MCAD
T1136 886-887 . denotes .
T1137 887-1711 sentence denotes Figure 1 Strategy for Disruption of the Mouse Acadm Gene (A) The MCAD IV2 insertion targeting vector with a deleted 1.3-kb region encompassing exon 10 and flanking sequences. MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele. (B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR. Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment. Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines. ES cell clones were screened by PCR (data not shown) and confirmed by Southern blot analysis.
T1138 1618-1620 NN denotes ES
T1139 1621-1625 NN denotes cell
T1140 1626-1632 NNS denotes clones
T1141 1638-1646 VBN denotes screened
T1142 1633-1637 VBD denotes were
T1143 1647-1649 IN denotes by
T1144 1650-1653 NN denotes PCR
T1145 1654-1655 -LRB- denotes (
T1146 1664-1669 VBN denotes shown
T1147 1655-1659 NNS denotes data
T1148 1660-1663 RB denotes not
T1149 1669-1670 -RRB- denotes )
T1150 1671-1674 CC denotes and
T1151 1675-1684 VBN denotes confirmed
T1152 1685-1687 IN denotes by
T1153 1688-1696 NNP denotes Southern
T1154 1697-1701 NN denotes blot
T1155 1702-1710 NN denotes analysis
T1156 1710-1711 . denotes .
T1157 1711-1951 sentence denotes Southern blot analysis used an exon 10 probe (probe A), not present in the targeting vector, hybridized to a 13.2-kb band in addition to the 3.1-kb endogenous band indicating targeted insertion of the vector at the Acadm locus (Figure 1B).
T1158 1712-1720 NNP denotes Southern
T1159 1721-1725 NN denotes blot
T1160 1726-1734 NN denotes analysis
T1161 1735-1739 VBD denotes used
T1162 1740-1742 DT denotes an
T1163 1751-1756 NN denotes probe
T1164 1743-1747 NN denotes exon
T1165 1748-1750 CD denotes 10
T1166 1757-1758 -LRB- denotes (
T1167 1764-1765 NN denotes A
T1168 1758-1763 NN denotes probe
T1169 1765-1766 -RRB- denotes )
T1170 1766-1768 , denotes ,
T1171 1768-1771 RB denotes not
T1172 1772-1779 JJ denotes present
T1173 1780-1782 IN denotes in
T1174 1783-1786 DT denotes the
T1175 1797-1803 NN denotes vector
T1176 1787-1796 NN denotes targeting
T1177 1803-1805 , denotes ,
T1178 1805-1815 VBN denotes hybridized
T1179 1816-1818 IN denotes to
T1180 1819-1820 DT denotes a
T1181 1829-1833 NN denotes band
T1182 1821-1825 CD denotes 13.2
T1183 1826-1828 NN denotes kb
T1184 1825-1826 HYPH denotes -
T1185 1834-1836 IN denotes in
T1186 1837-1845 NN denotes addition
T1187 1846-1848 IN denotes to
T1188 1849-1852 DT denotes the
T1189 1871-1875 NN denotes band
T1190 1853-1856 CD denotes 3.1
T1191 1857-1859 NN denotes kb
T1192 1856-1857 HYPH denotes -
T1193 1860-1870 JJ denotes endogenous
T1194 1876-1886 VBG denotes indicating
T1195 1887-1895 VBN denotes targeted
T1196 1896-1905 NN denotes insertion
T1197 1906-1908 IN denotes of
T1198 1909-1912 DT denotes the
T1199 1913-1919 NN denotes vector
T1200 1920-1922 IN denotes at
T1201 1923-1926 DT denotes the
T1202 1933-1938 NN denotes locus
T1203 1927-1932 NN denotes Acadm
T1204 1939-1940 -LRB- denotes (
T1205 1940-1946 NN denotes Figure
T1206 1947-1949 CD denotes 1B
T1207 1949-1950 -RRB- denotes )
T1208 1950-1951 . denotes .
T1209 1951-2065 sentence denotes Correctly targeted ES cell clones were microinjected into B6 (C57BL/6NTac) blastocysts to generate chimeric mice.
T1210 1952-1961 RB denotes Correctly
T1211 1962-1970 VBN denotes targeted
T1212 1979-1985 NNS denotes clones
T1213 1971-1973 NN denotes ES
T1214 1974-1978 NN denotes cell
T1215 1991-2004 VBN denotes microinjected
T1216 1986-1990 VBD denotes were
T1217 2005-2009 IN denotes into
T1218 2010-2012 NN denotes B6
T1219 2027-2038 NNS denotes blastocysts
T1220 2013-2014 -LRB- denotes (
T1221 2014-2019 NN denotes C57BL
T1222 2020-2025 NN denotes 6NTac
T1223 2019-2020 HYPH denotes /
T1224 2025-2026 -RRB- denotes )
T1225 2039-2041 TO denotes to
T1226 2042-2050 VB denotes generate
T1227 2051-2059 JJ denotes chimeric
T1228 2060-2064 NNS denotes mice
T1229 2064-2065 . denotes .
T1230 2065-2206 sentence denotes Chimeric mice were backcrossed to both 129P2 and B6 inbred mice to produce MCAD+/− and eventually MCAD−/− mice on a B6/129 mixed background.
T1231 2066-2074 JJ denotes Chimeric
T1232 2075-2079 NNS denotes mice
T1233 2085-2096 VBN denotes backcrossed
T1234 2080-2084 VBD denotes were
T1235 2097-2099 IN denotes to
T1236 2100-2104 CC denotes both
T1237 2105-2110 NN denotes 129P2
T1238 2118-2124 VBN denotes inbred
T1239 2111-2114 CC denotes and
T1240 2115-2117 NN denotes B6
T1241 2125-2129 NNS denotes mice
T1242 2130-2132 TO denotes to
T1243 2133-2140 VB denotes produce
T1244 2141-2145 NN denotes MCAD
T1245 2172-2176 NNS denotes mice
T1246 2145-2146 SYM denotes +
T1247 2146-2147 HYPH denotes /
T1248 2147-2148 SYM denotes −
T1249 2149-2152 CC denotes and
T1250 2153-2163 RB denotes eventually
T1251 2164-2168 NN denotes MCAD
T1252 2168-2169 SYM denotes −
T1253 2169-2170 HYPH denotes /
T1254 2170-2171 SYM denotes −
T1255 2177-2179 IN denotes on
T1256 2180-2181 DT denotes a
T1257 2195-2205 NN denotes background
T1258 2182-2184 NN denotes B6
T1259 2184-2185 HYPH denotes /
T1260 2185-2188 CD denotes 129
T1261 2189-2194 JJ denotes mixed
T1262 2205-2206 . denotes .
T1263 2206-2398 sentence denotes The studies described here were conducted exclusively on the B6/129 mixed background compared with littermate controls or B6/129 control groups maintained by intercrosses as were the mutants.
T1264 2207-2210 DT denotes The
T1265 2211-2218 NNS denotes studies
T1266 2239-2248 VBN denotes conducted
T1267 2219-2228 VBN denotes described
T1268 2229-2233 RB denotes here
T1269 2234-2238 VBD denotes were
T1270 2249-2260 RB denotes exclusively
T1271 2261-2263 IN denotes on
T1272 2264-2267 DT denotes the
T1273 2281-2291 NN denotes background
T1274 2268-2270 NN denotes B6
T1275 2270-2271 HYPH denotes /
T1276 2271-2274 CD denotes 129
T1277 2275-2280 JJ denotes mixed
T1278 2292-2300 VBN denotes compared
T1279 2301-2305 IN denotes with
T1280 2306-2316 NN denotes littermate
T1281 2317-2325 NNS denotes controls
T1282 2326-2328 CC denotes or
T1283 2329-2331 NN denotes B6
T1284 2344-2350 NNS denotes groups
T1285 2331-2332 HYPH denotes /
T1286 2332-2335 CD denotes 129
T1287 2336-2343 NN denotes control
T1288 2351-2361 VBN denotes maintained
T1289 2362-2364 IN denotes by
T1290 2365-2377 NNS denotes intercrosses
T1291 2378-2380 IN denotes as
T1292 2381-2385 VBD denotes were
T1293 2386-2389 DT denotes the
T1294 2390-2397 NNS denotes mutants
T1295 2397-2398 . denotes .
T1296 2398-2495 sentence denotes Perpetuating this mutation as a congenic mutant line on the 129P2 background proved impractical.
T1297 2399-2411 VBG denotes Perpetuating
T1298 2476-2482 VBD denotes proved
T1299 2412-2416 DT denotes this
T1300 2417-2425 NN denotes mutation
T1301 2426-2428 IN denotes as
T1302 2429-2430 DT denotes a
T1303 2447-2451 NN denotes line
T1304 2431-2439 JJ denotes congenic
T1305 2440-2446 NN denotes mutant
T1306 2452-2454 IN denotes on
T1307 2455-2458 DT denotes the
T1308 2465-2475 NN denotes background
T1309 2459-2464 NN denotes 129P2
T1310 2483-2494 JJ denotes impractical
T1311 2494-2495 . denotes .
T1312 2495-2663 sentence denotes The 129P2 mice were poor breeders as wild-types, and when introduced, the Acadm mutation was nearly lost on this background because of the high rate of neonatal death.
T1313 2496-2499 DT denotes The
T1314 2506-2510 NNS denotes mice
T1315 2500-2505 NN denotes 129P2
T1316 2511-2515 VBD denotes were
T1317 2516-2520 JJ denotes poor
T1318 2521-2529 NNS denotes breeders
T1319 2530-2532 IN denotes as
T1320 2533-2537 JJ denotes wild
T1321 2538-2543 NNS denotes types
T1322 2537-2538 HYPH denotes -
T1323 2543-2545 , denotes ,
T1324 2545-2548 CC denotes and
T1325 2549-2553 WRB denotes when
T1326 2554-2564 VBN denotes introduced
T1327 2596-2600 VBN denotes lost
T1328 2564-2566 , denotes ,
T1329 2566-2569 DT denotes the
T1330 2576-2584 NN denotes mutation
T1331 2570-2575 NN denotes Acadm
T1332 2585-2588 VBD denotes was
T1333 2589-2595 RB denotes nearly
T1334 2601-2603 IN denotes on
T1335 2604-2608 DT denotes this
T1336 2609-2619 NN denotes background
T1337 2620-2627 IN denotes because
T1338 2628-2630 IN denotes of
T1339 2631-2634 DT denotes the
T1340 2640-2644 NN denotes rate
T1341 2635-2639 JJ denotes high
T1342 2645-2647 IN denotes of
T1343 2648-2656 JJ denotes neonatal
T1344 2657-2662 NN denotes death
T1345 2662-2663 . denotes .
T1346 2663-2799 sentence denotes Because of the molecular structure of the targeted allele, it proved virtually impossible to distinguish all three potential genotypes.
T1347 2664-2671 IN denotes Because
T1348 2726-2732 VBD denotes proved
T1349 2672-2674 IN denotes of
T1350 2675-2678 DT denotes the
T1351 2689-2698 NN denotes structure
T1352 2679-2688 JJ denotes molecular
T1353 2699-2701 IN denotes of
T1354 2702-2705 DT denotes the
T1355 2715-2721 NN denotes allele
T1356 2706-2714 VBN denotes targeted
T1357 2721-2723 , denotes ,
T1358 2723-2725 PRP denotes it
T1359 2733-2742 RB denotes virtually
T1360 2743-2753 JJ denotes impossible
T1361 2754-2756 TO denotes to
T1362 2757-2768 VB denotes distinguish
T1363 2769-2772 DT denotes all
T1364 2789-2798 NNS denotes genotypes
T1365 2773-2778 CD denotes three
T1366 2779-2788 JJ denotes potential
T1367 2798-2799 . denotes .
T1368 2799-2994 sentence denotes We could clearly detect the presence or absence of the targeted allele, however, whether a particular mouse was MCAD−/− or MCAD+/− could not be determined by Southern blot or PCR of genomic DNA.
T1369 2800-2802 PRP denotes We
T1370 2817-2823 VB denotes detect
T1371 2803-2808 MD denotes could
T1372 2809-2816 RB denotes clearly
T1373 2944-2954 VBN denotes determined
T1374 2824-2827 DT denotes the
T1375 2828-2836 NN denotes presence
T1376 2837-2839 CC denotes or
T1377 2840-2847 NN denotes absence
T1378 2848-2850 IN denotes of
T1379 2851-2854 DT denotes the
T1380 2864-2870 NN denotes allele
T1381 2855-2863 VBN denotes targeted
T1382 2870-2872 , denotes ,
T1383 2872-2879 RB denotes however
T1384 2879-2881 , denotes ,
T1385 2881-2888 IN denotes whether
T1386 2908-2911 VBD denotes was
T1387 2889-2890 DT denotes a
T1388 2902-2907 NN denotes mouse
T1389 2891-2901 JJ denotes particular
T1390 2912-2916 NN denotes MCAD
T1391 2916-2917 SYM denotes −
T1392 2917-2918 HYPH denotes /
T1393 2918-2919 SYM denotes −
T1394 2920-2922 CC denotes or
T1395 2923-2927 NN denotes MCAD
T1396 2927-2928 SYM denotes +
T1397 2928-2929 HYPH denotes /
T1398 2929-2930 SYM denotes −
T1399 2931-2936 MD denotes could
T1400 2937-2940 RB denotes not
T1401 2941-2943 VB denotes be
T1402 2955-2957 IN denotes by
T1403 2958-2966 NNP denotes Southern
T1404 2967-2971 NN denotes blot
T1405 2972-2974 CC denotes or
T1406 2975-2978 NN denotes PCR
T1407 2979-2981 IN denotes of
T1408 2982-2989 JJ denotes genomic
T1409 2990-2993 NN denotes DNA
T1410 2993-2994 . denotes .
T1411 2994-3148 sentence denotes Ultimately MCAD−/− mice were ascertained by immunoblot analysis of offspring with subsequent perpetuation of MCAD−/− and MCAD+/+ mice as separate groups.
T1412 2995-3005 RB denotes Ultimately
T1413 3024-3035 VBN denotes ascertained
T1414 3006-3010 NN denotes MCAD
T1415 3014-3018 NNS denotes mice
T1416 3010-3011 SYM denotes −
T1417 3011-3012 HYPH denotes /
T1418 3012-3013 SYM denotes −
T1419 3019-3023 VBD denotes were
T1420 3036-3038 IN denotes by
T1421 3039-3049 NN denotes immunoblot
T1422 3050-3058 NN denotes analysis
T1423 3059-3061 IN denotes of
T1424 3062-3071 NN denotes offspring
T1425 3072-3076 IN denotes with
T1426 3077-3087 JJ denotes subsequent
T1427 3088-3100 NN denotes perpetuation
T1428 3101-3103 IN denotes of
T1429 3104-3108 NN denotes MCAD
T1430 3124-3128 NNS denotes mice
T1431 3108-3109 SYM denotes −
T1432 3109-3110 HYPH denotes /
T1433 3110-3111 SYM denotes −
T1434 3112-3115 CC denotes and
T1435 3116-3120 NN denotes MCAD
T1436 3120-3121 SYM denotes +
T1437 3121-3122 HYPH denotes /
T1438 3122-3123 SYM denotes +
T1439 3129-3131 IN denotes as
T1440 3132-3140 JJ denotes separate
T1441 3141-3147 NNS denotes groups
T1442 3147-3148 . denotes .
T7247 898-906 NN denotes Strategy
T7248 907-910 IN denotes for
T7249 911-921 NN denotes Disruption
T7250 922-924 IN denotes of
T7251 925-928 DT denotes the
T7252 941-945 NN denotes Gene
T7253 929-934 NN denotes Mouse
T7254 935-940 NN denotes Acadm
T7255 945-1063 sentence denotes (A) The MCAD IV2 insertion targeting vector with a deleted 1.3-kb region encompassing exon 10 and flanking sequences.
T7256 946-947 -LRB- denotes (
T7257 947-948 LS denotes A
T7258 983-989 NN denotes vector
T7259 948-949 -RRB- denotes )
T7260 950-953 DT denotes The
T7261 954-958 NN denotes MCAD
T7262 963-972 NN denotes insertion
T7263 959-962 NN denotes IV2
T7264 973-982 NN denotes targeting
T7265 990-994 IN denotes with
T7266 995-996 DT denotes a
T7267 1012-1018 NN denotes region
T7268 997-1004 VBN denotes deleted
T7269 1005-1008 CD denotes 1.3
T7270 1009-1011 NN denotes kb
T7271 1008-1009 HYPH denotes -
T7272 1019-1031 VBG denotes encompassing
T7273 1032-1036 NN denotes exon
T7274 1037-1039 CD denotes 10
T7275 1040-1043 CC denotes and
T7276 1044-1052 VBG denotes flanking
T7277 1053-1062 NNS denotes sequences
T7278 1062-1063 . denotes .
T7279 1063-1227 sentence denotes MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele.
T7280 1064-1068 NN denotes MCAD
T7281 1069-1072 NN denotes IV2
T7282 1073-1082 VBZ denotes undergoes
T7283 1083-1086 NN denotes gap
T7284 1087-1093 NN denotes repair
T7285 1094-1098 IN denotes upon
T7286 1099-1109 JJ denotes homologous
T7287 1110-1123 NN denotes recombination
T7288 1124-1126 IN denotes at
T7289 1127-1130 DT denotes the
T7290 1148-1153 NN denotes locus
T7291 1131-1141 JJ denotes endogenous
T7292 1142-1147 NN denotes Acadm
T7293 1154-1163 VBG denotes resulting
T7294 1164-1166 IN denotes in
T7295 1167-1168 DT denotes a
T7296 1169-1180 NN denotes duplication
T7297 1181-1183 IN denotes of
T7298 1184-1189 NNS denotes exons
T7299 1190-1191 CD denotes 8
T7300 1191-1193 , denotes ,
T7301 1193-1194 CD denotes 9
T7302 1194-1196 , denotes ,
T7303 1196-1199 CC denotes and
T7304 1200-1202 CD denotes 10
T7305 1203-1205 IN denotes at
T7306 1206-1209 DT denotes the
T7307 1220-1226 NN denotes allele
T7308 1210-1219 VBN denotes disrupted
T7309 1226-1227 . denotes .
T7310 1227-1315 sentence denotes (B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR.
T7311 1228-1229 -LRB- denotes (
T7312 1229-1230 LS denotes B
T7313 1246-1254 NN denotes analysis
T7314 1230-1231 -RRB- denotes )
T7315 1232-1240 NNP denotes Southern
T7316 1241-1245 NN denotes blot
T7317 1255-1257 IN denotes of
T7318 1258-1263 NN denotes EcoRI
T7319 1264-1272 VBN denotes digested
T7320 1263-1264 HYPH denotes -
T7321 1281-1284 NN denotes DNA
T7322 1273-1280 JJ denotes genomic
T7323 1285-1289 IN denotes from
T7324 1290-1292 NN denotes ES
T7325 1293-1298 NNS denotes cells
T7326 1299-1307 VBN denotes screened
T7327 1308-1310 IN denotes by
T7328 1311-1314 NN denotes PCR
T7329 1314-1315 . denotes .
T7330 1315-1522 sentence denotes Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment.
T7331 1316-1321 NN denotes Probe
T7332 1322-1323 NN denotes A
T7333 1420-1430 VBZ denotes hybridizes
T7334 1323-1325 , denotes ,
T7335 1325-1326 DT denotes a
T7336 1331-1339 NN denotes fragment
T7337 1327-1330 NN denotes DNA
T7338 1340-1350 VBG denotes consisting
T7339 1351-1353 IN denotes of
T7340 1354-1355 DT denotes a
T7341 1356-1363 NN denotes portion
T7342 1364-1366 IN denotes of
T7343 1367-1371 NN denotes exon
T7344 1372-1374 CD denotes 10
T7345 1375-1379 WDT denotes that
T7346 1380-1382 VBZ denotes is
T7347 1383-1386 RB denotes not
T7348 1387-1394 JJ denotes present
T7349 1395-1397 IN denotes in
T7350 1398-1401 DT denotes the
T7351 1412-1418 NN denotes vector
T7352 1402-1411 NN denotes targeting
T7353 1418-1420 , denotes ,
T7354 1431-1433 IN denotes to
T7355 1434-1436 DT denotes an
T7356 1455-1463 NN denotes fragment
T7357 1437-1447 JJ denotes endogenous
T7358 1448-1451 CD denotes 3.1
T7359 1452-1454 NN denotes kb
T7360 1451-1452 HYPH denotes -
T7361 1464-1467 CC denotes and
T7362 1467-1469 , denotes ,
T7363 1469-1473 IN denotes upon
T7364 1500-1502 IN denotes to
T7365 1474-1484 JJ denotes homologous
T7366 1485-1498 NN denotes recombination
T7367 1498-1500 , denotes ,
T7368 1503-1504 DT denotes a
T7369 1513-1521 NN denotes fragment
T7370 1505-1509 CD denotes 13.2
T7371 1510-1512 NN denotes kb
T7372 1509-1510 HYPH denotes -
T7373 1521-1522 . denotes .
T7374 1522-1617 sentence denotes Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines.
T7375 1523-1527 NN denotes Lane
T7376 1530-1540 VBZ denotes represents
T7377 1528-1529 CD denotes 1
T7378 1541-1542 DT denotes a
T7379 1561-1565 NN denotes line
T7380 1543-1547 JJ denotes wild
T7381 1548-1552 NN denotes type
T7382 1547-1548 HYPH denotes -
T7383 1553-1555 NN denotes ES
T7384 1556-1560 NN denotes cell
T7385 1565-1567 , denotes ,
T7386 1567-1570 CC denotes and
T7387 1571-1575 NN denotes Lane
T7388 1576-1577 CD denotes 2
T7389 1584-1593 VBP denotes represent
T7390 1578-1581 CC denotes and
T7391 1582-1583 CD denotes 3
T7392 1594-1602 VBN denotes targeted
T7393 1611-1616 NNS denotes lines
T7394 1603-1605 NN denotes ES
T7395 1606-1610 NN denotes cell
T7396 1616-1617 . denotes .
R579 T964 T965 compound Gene,Targeting
R580 T966 T965 cc and,Targeting
R581 T967 T965 conj Generation,Targeting
R582 T968 T967 prep of,Generation
R583 T969 T970 npadvmod MCAD,Deficient
R584 T970 T972 amod Deficient,Mice
R585 T971 T970 punct -,Deficient
R586 T972 T968 pobj Mice,of
R587 T974 T975 compound MCAD,vector
R588 T975 T977 nsubjpass vector,designed
R589 T976 T975 compound insertion,vector
R590 T978 T975 punct (,vector
R591 T979 T980 compound MCAD,IV2
R592 T980 T975 appos IV2,vector
R593 T981 T977 punct ),designed
R594 T982 T977 auxpass was,designed
R595 T983 T984 aux to,undergo
R596 T984 T977 advcl undergo,designed
R597 T985 T986 compound gap,repair
R598 T986 T984 dobj repair,undergo
R599 T987 T986 prep of,repair
R600 T988 T989 det the,region
R601 T989 T987 pobj region,of
R602 T990 T991 nummod 1.3,kb
R603 T991 T989 nmod kb,region
R604 T992 T991 punct -,kb
R605 T993 T989 amod deleted,region
R606 T994 T984 prep upon,undergo
R607 T995 T996 amod homologous,recombination
R608 T996 T994 pobj recombination,upon
R609 T997 T984 prep in,undergo
R610 T998 T999 nmod 129P2,cells
R611 T999 T997 pobj cells,in
R612 T1000 T1001 punct (,OlaHsd
R613 T1001 T999 parataxis OlaHsd,cells
R614 T1002 T1001 compound 129P2,OlaHsd
R615 T1003 T1001 punct /,OlaHsd
R616 T1004 T1001 punct ),OlaHsd
R617 T1005 T999 compound ES,cells
R618 T1006 T999 appos E14,cells
R619 T1007 T1006 punct –,E14
R620 T1008 T1006 nummod 1,E14
R621 T1009 T977 punct .,designed
R622 T1011 T1012 amod Correct,targeting
R623 T1012 T1013 nsubj targeting,resulted
R624 T1014 T1012 prep of,targeting
R625 T1015 T1016 det the,locus
R626 T1016 T1014 pobj locus,of
R627 T1017 T1016 compound MCAD,locus
R628 T1018 T1013 prep in,resulted
R629 T1019 T1020 det a,duplication
R630 T1020 T1018 pobj duplication,in
R631 T1021 T1020 prep of,duplication
R632 T1022 T1023 nmod exons,8
R633 T1023 T1021 pobj 8,of
R634 T1024 T1023 punct ", ",8
R635 T1025 T1023 conj 9,8
R636 T1026 T1025 punct ", ",9
R637 T1027 T1025 cc and,9
R638 T1028 T1025 conj 10,9
R639 T1029 T1020 cc and,duplication
R640 T1030 T1020 conj integration,duplication
R641 T1031 T1030 prep of,integration
R642 T1032 T1033 amod flanking,plasmid
R643 T1033 T1031 pobj plasmid,of
R644 T1034 T1033 cc and,plasmid
R645 T1035 T1036 compound Neo,sequences
R646 T1036 T1033 conj sequences,plasmid
R647 T1037 T1038 punct (,Figure
R648 T1038 T1013 parataxis Figure,resulted
R649 T1039 T1038 nummod 1A,Figure
R650 T1040 T1038 punct ),Figure
R651 T1041 T1013 punct .,resulted
R652 T1043 T1044 det The,vector
R653 T1044 T1046 nsubjpass vector,designed
R654 T1045 T1044 compound insertion,vector
R655 T1047 T1046 auxpass was,designed
R656 T1048 T1049 aux to,duplicate
R657 T1049 T1046 advcl duplicate,designed
R658 T1050 T1051 nmod exon,8
R659 T1051 T1049 dobj 8,duplicate
R660 T1052 T1051 punct ", ",8
R661 T1053 T1051 conj 9,8
R662 T1054 T1053 punct ", ",9
R663 T1055 T1053 cc and,9
R664 T1056 T1053 conj 10,9
R665 T1057 T1049 prep at,duplicate
R666 T1058 T1059 det the,locus
R667 T1059 T1057 pobj locus,at
R668 T1060 T1059 compound MCAD,locus
R669 T1061 T1046 punct .,designed
R670 T1063 T1064 nsubj Translation,results
R671 T1065 T1063 prep of,Translation
R672 T1066 T1067 det the,region
R674 T1068 T1067 amod duplicated,region
R690 T1084 T1083 compound MCAD,monomer
R691 T1085 T1064 punct .,results
R692 T1087 T1088 advmod Specifically,arises
R693 T1089 T1088 punct ", ",arises
R694 T1090 T1091 det the,codon
R695 T1091 T1088 nsubj codon,arises
R696 T1092 T1091 amod first,codon
R697 T1093 T1091 amod premature,codon
R698 T1094 T1091 compound stop,codon
R699 T1095 T1088 prep after,arises
R700 T1096 T1095 pobj translation,after
R701 T1097 T1096 prep of,translation
R702 T1098 T1099 advmod only,seven
R703 T1099 T1100 nummod seven,acids
R704 T1100 T1097 pobj acids,of
R705 T1101 T1100 compound amino,acids
R706 T1102 T1100 prep from,acids
R707 T1103 T1104 det the,exon
R708 T1104 T1102 pobj exon,from
R709 T1105 T1104 amod duplicated,exon
R710 T1106 T1104 nummod 8,exon
R711 T1107 T1088 punct .,arises
R712 T1109 T1110 det The,monomer
R713 T1110 T1113 nsubj monomer,missing
R714 T1111 T1110 amod resulting,monomer
R715 T1112 T1110 compound MCAD,monomer
R716 T1114 T1113 aux is,missing
R717 T1115 T1116 det the,helixes
R718 T1116 T1113 dobj helixes,missing
R719 T1117 T1118 npadvmod C,terminal
R720 T1118 T1120 amod terminal,domain
R721 T1119 T1118 punct -,terminal
R722 T1120 T1116 compound domain,helixes
R723 T1121 T1116 compound α,helixes
R724 T1122 T1116 punct -,helixes
R725 T1123 T1124 dep that,are
R726 T1124 T1116 relcl are,helixes
R727 T1125 T1124 acomp responsible,are
R728 T1126 T1125 prep for,responsible
R729 T1127 T1126 pcomp making,for
R730 T1128 T1129 compound intersubunit,contacts
R731 T1129 T1127 dobj contacts,making
R732 T1130 T1131 aux to,generate
R733 T1131 T1127 advcl generate,making
R734 T1132 T1133 det the,homotetramer
R735 T1133 T1131 dobj homotetramer,generate
R736 T1134 T1133 amod functional,homotetramer
R737 T1135 T1133 compound MCAD,homotetramer
R738 T1136 T1113 punct .,missing
R775 T1177 T1163 punct ", ",probe
R776 T1178 T1163 acl hybridized,probe
R777 T1179 T1178 prep to,hybridized
R778 T1180 T1181 det a,band
R779 T1181 T1179 pobj band,to
R780 T1182 T1183 nummod 13.2,kb
R781 T1183 T1181 compound kb,band
R782 T1184 T1183 punct -,kb
R783 T1185 T1178 prep in,hybridized
R784 T1186 T1185 pobj addition,in
R785 T1187 T1186 prep to,addition
R786 T1188 T1189 det the,band
R787 T1189 T1187 pobj band,to
R788 T1190 T1191 nummod 3.1,kb
R789 T1191 T1189 nmod kb,band
R790 T1192 T1191 punct -,kb
R791 T1193 T1189 amod endogenous,band
R792 T1194 T1189 acl indicating,band
R793 T1195 T1196 amod targeted,insertion
R794 T1196 T1194 dobj insertion,indicating
R795 T1197 T1196 prep of,insertion
R796 T1198 T1199 det the,vector
R797 T1199 T1197 pobj vector,of
R798 T1200 T1196 prep at,insertion
R799 T1201 T1202 det the,locus
R800 T1202 T1200 pobj locus,at
R801 T1203 T1202 compound Acadm,locus
R802 T1204 T1205 punct (,Figure
R803 T1205 T1161 parataxis Figure,used
R804 T1206 T1205 nummod 1B,Figure
R805 T1207 T1205 punct ),Figure
R806 T1208 T1161 punct .,used
R807 T1210 T1211 advmod Correctly,targeted
R808 T1211 T1212 amod targeted,clones
R809 T1212 T1215 nsubjpass clones,microinjected
R810 T1213 T1214 compound ES,cell
R811 T1214 T1212 compound cell,clones
R812 T1216 T1215 auxpass were,microinjected
R813 T1217 T1215 prep into,microinjected
R814 T1218 T1219 nmod B6,blastocysts
R815 T1219 T1217 pobj blastocysts,into
R816 T1220 T1219 punct (,blastocysts
R817 T1221 T1222 nmod C57BL,6NTac
R818 T1222 T1219 nmod 6NTac,blastocysts
R819 T1223 T1222 punct /,6NTac
R820 T1224 T1219 punct ),blastocysts
R821 T1225 T1226 aux to,generate
R822 T1226 T1215 advcl generate,microinjected
R823 T1227 T1228 amod chimeric,mice
R824 T1228 T1226 dobj mice,generate
R825 T1229 T1215 punct .,microinjected
R826 T1231 T1232 amod Chimeric,mice
R827 T1232 T1233 nsubjpass mice,backcrossed
R828 T1234 T1233 auxpass were,backcrossed
R829 T1235 T1233 prep to,backcrossed
R830 T1236 T1237 preconj both,129P2
R831 T1237 T1238 npadvmod 129P2,inbred
R832 T1238 T1241 amod inbred,mice
R833 T1239 T1237 cc and,129P2
R834 T1240 T1237 conj B6,129P2
R835 T1241 T1235 pobj mice,to
R836 T1242 T1243 aux to,produce
R837 T1243 T1233 advcl produce,backcrossed
R838 T1244 T1245 nmod MCAD,mice
R839 T1245 T1243 dobj mice,produce
R840 T1246 T1244 punct +,MCAD
R841 T1247 T1244 punct /,MCAD
R842 T1248 T1244 punct −,MCAD
R843 T1249 T1244 cc and,MCAD
R844 T1250 T1251 advmod eventually,MCAD
R845 T1251 T1244 conj MCAD,MCAD
R846 T1252 T1251 punct −,MCAD
R847 T1253 T1251 punct /,MCAD
R848 T1254 T1251 punct −,MCAD
R849 T1255 T1243 prep on,produce
R850 T1256 T1257 det a,background
R851 T1257 T1255 pobj background,on
R852 T1258 T1257 nmod B6,background
R853 T1259 T1258 punct /,B6
R854 T1260 T1258 nummod 129,B6
R855 T1261 T1257 amod mixed,background
R856 T1262 T1233 punct .,backcrossed
R857 T1264 T1265 det The,studies
R858 T1265 T1266 nsubjpass studies,conducted
R859 T1267 T1265 acl described,studies
R860 T1268 T1267 advmod here,described
R861 T1269 T1266 auxpass were,conducted
R862 T1270 T1266 advmod exclusively,conducted
R863 T1271 T1266 prep on,conducted
R864 T1272 T1273 det the,background
R865 T1273 T1271 pobj background,on
R866 T1274 T1273 nmod B6,background
R867 T1275 T1274 punct /,B6
R868 T1276 T1274 nummod 129,B6
R869 T1277 T1273 amod mixed,background
R870 T1278 T1273 prep compared,background
R871 T1279 T1278 prep with,compared
R872 T1280 T1281 compound littermate,controls
R873 T1281 T1279 pobj controls,with
R874 T1282 T1281 cc or,controls
R875 T1283 T1284 nmod B6,groups
R876 T1284 T1281 conj groups,controls
R877 T1285 T1283 punct /,B6
R878 T1286 T1283 nummod 129,B6
R879 T1287 T1284 compound control,groups
R880 T1288 T1281 acl maintained,controls
R881 T1289 T1288 prep by,maintained
R882 T1290 T1289 pobj intercrosses,by
R883 T1291 T1292 mark as,were
R884 T1292 T1288 advcl were,maintained
R885 T1293 T1294 det the,mutants
R886 T1294 T1292 nsubj mutants,were
R887 T1295 T1266 punct .,conducted
R888 T1297 T1298 csubj Perpetuating,proved
R889 T1299 T1300 det this,mutation
R890 T1300 T1297 dobj mutation,Perpetuating
R891 T1301 T1297 prep as,Perpetuating
R892 T1302 T1303 det a,line
R893 T1303 T1301 pobj line,as
R894 T1304 T1303 amod congenic,line
R895 T1305 T1303 compound mutant,line
R896 T1306 T1303 prep on,line
R897 T1307 T1308 det the,background
R898 T1308 T1306 pobj background,on
R899 T1309 T1308 compound 129P2,background
R900 T1310 T1298 oprd impractical,proved
R901 T1311 T1298 punct .,proved
R902 T1313 T1314 det The,mice
R903 T1314 T1316 nsubj mice,were
R904 T1315 T1314 compound 129P2,mice
R905 T1317 T1318 amod poor,breeders
R906 T1318 T1316 attr breeders,were
R907 T1319 T1316 prep as,were
R908 T1320 T1321 amod wild,types
R909 T1321 T1319 pobj types,as
R910 T1322 T1321 punct -,types
R911 T1323 T1316 punct ", ",were
R912 T1324 T1316 cc and,were
R913 T1325 T1326 advmod when,introduced
R914 T1326 T1327 advcl introduced,lost
R915 T1327 T1316 conj lost,were
R916 T1328 T1327 punct ", ",lost
R917 T1329 T1330 det the,mutation
R918 T1330 T1327 nsubjpass mutation,lost
R919 T1331 T1330 compound Acadm,mutation
R920 T1332 T1327 auxpass was,lost
R921 T1333 T1327 advmod nearly,lost
R922 T1334 T1327 prep on,lost
R923 T1335 T1336 det this,background
R924 T1336 T1334 pobj background,on
R925 T1337 T1327 prep because,lost
R926 T1338 T1337 pcomp of,because
R927 T1339 T1340 det the,rate
R928 T1340 T1337 pobj rate,because
R929 T1341 T1340 amod high,rate
R930 T1342 T1340 prep of,rate
R931 T1343 T1344 amod neonatal,death
R932 T1344 T1342 pobj death,of
R933 T1345 T1327 punct .,lost
R934 T1347 T1348 prep Because,proved
R935 T1349 T1347 pcomp of,Because
R936 T1350 T1351 det the,structure
R937 T1351 T1347 pobj structure,Because
R938 T1352 T1351 amod molecular,structure
R939 T1353 T1351 prep of,structure
R940 T1354 T1355 det the,allele
R941 T1355 T1353 pobj allele,of
R942 T1356 T1355 amod targeted,allele
R943 T1357 T1348 punct ", ",proved
R944 T1358 T1348 nsubj it,proved
R945 T1359 T1360 advmod virtually,impossible
R946 T1360 T1348 oprd impossible,proved
R947 T1361 T1362 aux to,distinguish
R948 T1362 T1348 xcomp distinguish,proved
R949 T1363 T1364 det all,genotypes
R950 T1364 T1362 dobj genotypes,distinguish
R951 T1365 T1364 nummod three,genotypes
R952 T1366 T1364 amod potential,genotypes
R953 T1367 T1348 punct .,proved
R954 T1369 T1370 nsubj We,detect
R955 T1370 T1373 ccomp detect,determined
R956 T1371 T1370 aux could,detect
R957 T1372 T1370 advmod clearly,detect
R958 T1374 T1375 det the,presence
R959 T1375 T1370 dobj presence,detect
R960 T1376 T1375 cc or,presence
R961 T1377 T1375 conj absence,presence
R962 T1378 T1375 prep of,presence
R963 T1379 T1380 det the,allele
R964 T1380 T1378 pobj allele,of
R965 T1381 T1380 amod targeted,allele
R966 T1382 T1373 punct ", ",determined
R967 T1383 T1373 advmod however,determined
R968 T1384 T1373 punct ", ",determined
R969 T1385 T1386 mark whether,was
R970 T1386 T1373 csubjpass was,determined
R971 T1387 T1388 det a,mouse
R972 T1388 T1386 nsubj mouse,was
R973 T1389 T1388 amod particular,mouse
R974 T1390 T1386 attr MCAD,was
R975 T1391 T1390 punct −,MCAD
R976 T1392 T1390 punct /,MCAD
R977 T1393 T1390 punct −,MCAD
R978 T1394 T1390 cc or,MCAD
R979 T1395 T1390 conj MCAD,MCAD
R980 T1396 T1395 punct +,MCAD
R981 T1397 T1395 punct /,MCAD
R982 T1398 T1395 punct −,MCAD
R983 T1399 T1373 aux could,determined
R984 T1400 T1373 neg not,determined
R985 T1401 T1373 auxpass be,determined
R986 T1402 T1373 prep by,determined
R987 T1403 T1404 compound Southern,blot
R988 T1404 T1402 pobj blot,by
R989 T1405 T1404 cc or,blot
R990 T1406 T1404 conj PCR,blot
R991 T1407 T1404 prep of,blot
R992 T1408 T1409 amod genomic,DNA
R993 T1409 T1407 pobj DNA,of
R994 T1410 T1373 punct .,determined
R995 T1412 T1413 advmod Ultimately,ascertained
R996 T1414 T1415 nmod MCAD,mice
R997 T1415 T1413 nsubjpass mice,ascertained
R998 T1416 T1414 punct −,MCAD
R999 T1417 T1414 punct /,MCAD
R1000 T1418 T1414 punct −,MCAD
R1001 T1419 T1413 auxpass were,ascertained
R1002 T1420 T1413 prep by,ascertained
R1003 T1421 T1422 compound immunoblot,analysis
R1004 T1422 T1420 pobj analysis,by
R1005 T1423 T1422 prep of,analysis
R1006 T1424 T1423 pobj offspring,of
R1007 T1425 T1422 prep with,analysis
R1008 T1426 T1427 amod subsequent,perpetuation
R1009 T1427 T1425 pobj perpetuation,with
R1010 T1428 T1427 prep of,perpetuation
R1011 T1429 T1430 nmod MCAD,mice
R1012 T1430 T1428 pobj mice,of
R1013 T1431 T1429 punct −,MCAD
R1014 T1432 T1429 punct /,MCAD
R1015 T1433 T1429 punct −,MCAD
R1016 T1434 T1429 cc and,MCAD
R1017 T1435 T1429 conj MCAD,MCAD
R1018 T1436 T1435 punct +,MCAD
R1019 T1437 T1435 punct /,MCAD
R1020 T1438 T1435 punct +,MCAD
R1021 T1439 T1427 prep as,perpetuation
R1022 T1440 T1441 amod separate,groups
R1023 T1441 T1439 pobj groups,as
R1024 T1442 T1413 punct .,ascertained
R4678 T7248 T7247 prep for,Strategy
R4679 T7249 T7248 pobj Disruption,for
R4680 T7250 T7249 prep of,Disruption
R4681 T7251 T7252 det the,Gene
R4682 T7252 T7250 pobj Gene,of
R4683 T7253 T7252 compound Mouse,Gene
R4684 T7254 T7252 compound Acadm,Gene
R4685 T7256 T7257 punct (,A
R4686 T7257 T7258 meta A,vector
R4687 T7259 T7257 punct ),A
R4688 T7260 T7258 det The,vector
R4689 T7261 T7262 compound MCAD,insertion
R4690 T7262 T7258 compound insertion,vector
R4691 T7263 T7262 compound IV2,insertion
R4692 T7264 T7258 compound targeting,vector
R4693 T7265 T7258 prep with,vector
R4694 T7266 T7267 det a,region
R4695 T7267 T7265 pobj region,with
R4696 T7268 T7267 amod deleted,region
R4697 T7269 T7270 nummod 1.3,kb
R4698 T7270 T7267 compound kb,region
R4699 T7271 T7270 punct -,kb
R4700 T7272 T7267 acl encompassing,region
R4701 T7273 T7272 dobj exon,encompassing
R4702 T7274 T7273 nummod 10,exon
R4703 T7275 T7273 cc and,exon
R4704 T7276 T7277 amod flanking,sequences
R4705 T7277 T7273 conj sequences,exon
R4706 T7278 T7258 punct .,vector
R4707 T7280 T7281 compound MCAD,IV2
R4708 T7281 T7282 nsubj IV2,undergoes
R4709 T7283 T7284 compound gap,repair
R4710 T7284 T7282 dobj repair,undergoes
R4711 T7285 T7282 prep upon,undergoes
R4712 T7286 T7287 amod homologous,recombination
R4713 T7287 T7285 pobj recombination,upon
R4714 T7288 T7282 prep at,undergoes
R4715 T7289 T7290 det the,locus
R4716 T7290 T7288 pobj locus,at
R4717 T7291 T7290 amod endogenous,locus
R4718 T7292 T7290 compound Acadm,locus
R4719 T7293 T7282 advcl resulting,undergoes
R4720 T7294 T7293 prep in,resulting
R4721 T7295 T7296 det a,duplication
R4722 T7296 T7294 pobj duplication,in
R4723 T7297 T7296 prep of,duplication
R4724 T7298 T7299 nmod exons,8
R4725 T7299 T7297 pobj 8,of
R4726 T7300 T7299 punct ", ",8
R4727 T7301 T7299 conj 9,8
R4728 T7302 T7301 punct ", ",9
R4729 T7303 T7301 cc and,9
R4730 T7304 T7301 conj 10,9
R4731 T7305 T7296 prep at,duplication
R4732 T7306 T7307 det the,allele
R4733 T7307 T7305 pobj allele,at
R4734 T7308 T7307 amod disrupted,allele
R4735 T7309 T7282 punct .,undergoes
R4736 T7311 T7312 punct (,B
R4737 T7312 T7313 meta B,analysis
R4738 T7314 T7312 punct ),B
R4739 T7315 T7316 compound Southern,blot
R4740 T7316 T7313 compound blot,analysis
R4741 T7317 T7313 prep of,analysis
R4742 T7318 T7319 npadvmod EcoRI,digested
R4743 T7319 T7321 amod digested,DNA
R4744 T7320 T7319 punct -,digested
R4745 T7321 T7317 pobj DNA,of
R4746 T7322 T7321 amod genomic,DNA
R4747 T7323 T7321 prep from,DNA
R4748 T7324 T7325 compound ES,cells
R4749 T7325 T7323 pobj cells,from
R4750 T7326 T7325 acl screened,cells
R4751 T7327 T7326 prep by,screened
R4752 T7328 T7327 pobj PCR,by
R4753 T7329 T7313 punct .,analysis
R4754 T7331 T7332 compound Probe,A
R4755 T7332 T7333 nsubj A,hybridizes
R4756 T7334 T7332 punct ", ",A
R4757 T7335 T7336 det a,fragment
R4758 T7336 T7332 appos fragment,A
R4759 T7337 T7336 compound DNA,fragment
R4760 T7338 T7336 acl consisting,fragment
R4761 T7339 T7338 prep of,consisting
R4762 T7340 T7341 det a,portion
R4763 T7341 T7339 pobj portion,of
R4764 T7342 T7341 prep of,portion
R4765 T7343 T7342 pobj exon,of
R4766 T7344 T7343 nummod 10,exon
R4767 T7345 T7346 dep that,is
R4768 T7346 T7341 relcl is,portion
R4769 T7347 T7346 neg not,is
R4770 T7348 T7346 acomp present,is
R4771 T7349 T7346 prep in,is
R4772 T7350 T7351 det the,vector
R4773 T7351 T7349 pobj vector,in
R4774 T7352 T7351 compound targeting,vector
R4775 T7353 T7333 punct ", ",hybridizes
R4776 T7354 T7333 prep to,hybridizes
R4777 T7355 T7356 det an,fragment
R4778 T7356 T7354 pobj fragment,to
R4779 T7357 T7356 amod endogenous,fragment
R4780 T7358 T7359 nummod 3.1,kb
R4781 T7359 T7356 compound kb,fragment
R4782 T7360 T7359 punct -,kb
R4783 T7361 T7333 cc and,hybridizes
R4784 T7362 T7333 punct ", ",hybridizes
R4785 T7363 T7364 prep upon,to
R4786 T7364 T7333 conj to,hybridizes
R4787 T7365 T7366 amod homologous,recombination
R4788 T7366 T7363 pobj recombination,upon
R4789 T7367 T7364 punct ", ",to
R4790 T7368 T7369 det a,fragment
R4791 T7369 T7364 pobj fragment,to
R4792 T7370 T7371 nummod 13.2,kb
R4793 T7371 T7369 compound kb,fragment
R4794 T7372 T7371 punct -,kb
R4795 T7373 T7333 punct .,hybridizes
R4796 T7375 T7376 nsubj Lane,represents
R4797 T7377 T7375 nummod 1,Lane
R4798 T7378 T7379 det a,line
R4799 T7379 T7376 dobj line,represents
R4800 T7380 T7381 amod wild,type
R4801 T7381 T7379 compound type,line
R4802 T7382 T7381 punct -,type
R4803 T7383 T7379 compound ES,line
R4804 T7384 T7379 compound cell,line
R4805 T7385 T7376 punct ", ",represents
R4806 T7386 T7376 cc and,represents
R4807 T7387 T7388 nmod Lane,2
R4808 T7388 T7389 nsubj 2,represent
R4809 T7389 T7376 conj represent,represents
R4810 T7390 T7388 cc and,2
R4811 T7391 T7388 conj 3,2
R4812 T7392 T7393 amod targeted,lines
R4813 T7393 T7389 dobj lines,represent
R4814 T7394 T7393 compound ES,lines
R4815 T7395 T7393 compound cell,lines
R4816 T7396 T7389 punct .,represent
R673 T1067 T1065 pobj region,of
R675 T1069 T1067 nmod exon,region
R676 T1070 T1069 nummod 8,exon
R677 T1071 T1064 prep in,results
R678 T1072 T1073 det the,formation
R679 T1073 T1071 pobj formation,in
R680 T1074 T1073 prep of,formation
R681 T1075 T1076 amod premature,codons
R682 T1076 T1074 pobj codons,of
R683 T1077 T1076 compound stop,codons
R684 T1078 T1073 acl resulting,formation
R685 T1079 T1078 prep in,resulting
R686 T1080 T1079 pobj truncation,in
R687 T1081 T1080 prep of,truncation
R688 T1082 T1083 det the,monomer
R689 T1083 T1081 pobj monomer,of
R739 T1138 T1139 compound ES,cell
R740 T1139 T1140 compound cell,clones
R741 T1140 T1141 nsubjpass clones,screened
R742 T1142 T1141 auxpass were,screened
R743 T1143 T1141 prep by,screened
R744 T1144 T1143 pobj PCR,by
R745 T1145 T1146 punct (,shown
R746 T1146 T1141 parataxis shown,screened
R747 T1147 T1146 nsubj data,shown
R748 T1148 T1146 neg not,shown
R749 T1149 T1146 punct ),shown
R750 T1150 T1141 cc and,screened
R751 T1151 T1141 conj confirmed,screened
R752 T1152 T1151 prep by,confirmed
R753 T1153 T1154 compound Southern,blot
R754 T1154 T1155 compound blot,analysis
R755 T1155 T1152 pobj analysis,by
R756 T1156 T1141 punct .,screened
R757 T1158 T1159 compound Southern,blot
R758 T1159 T1160 compound blot,analysis
R759 T1160 T1161 nsubj analysis,used
R760 T1162 T1163 det an,probe
R761 T1163 T1161 dobj probe,used
R762 T1164 T1163 nmod exon,probe
R763 T1165 T1164 nummod 10,exon
R764 T1166 T1167 punct (,A
R765 T1167 T1163 parataxis A,probe
R766 T1168 T1167 compound probe,A
R767 T1169 T1167 punct ),A
R768 T1170 T1163 punct ", ",probe
R769 T1171 T1172 neg not,present
R770 T1172 T1163 relcl present,probe
R771 T1173 T1172 prep in,present
R772 T1174 T1175 det the,vector
R773 T1175 T1173 pobj vector,in
R774 T1176 T1175 compound targeting,vector

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T1450 385-391 SO:0000440 denotes vector
T1451 418-422 SO:0000147 denotes exon
T1452 455-466 GO:0006412 denotes Translation
T1453 485-489 SO:0000147 denotes exon
T1454 527-548 SO:0001587 denotes premature stop codons
T1455 618-638 SO:0001587 denotes premature stop codon
T1456 652-663 GO:0006412 denotes translation
T1457 710-714 SO:0000147 denotes exon
T1458 771-777 SO:0000417 denotes domain
T1459 778-787 SO:0001117 denotes α-helixes
T1460 1618-1625 CL:0002322 denotes ES cell
T1461 1743-1747 SO:0000147 denotes exon
T1462 1787-1803 SO:0001644 denotes targeting vector
T1463 1805-1815 GO:0097617 denotes hybridized
T1464 1913-1919 SO:0000440 denotes vector
T1465 1971-1978 CL:0002322 denotes ES cell
T1466 2060-2064 NCBITaxon:10088 denotes mice
T1467 2075-2079 NCBITaxon:10088 denotes mice
T1468 2125-2129 NCBITaxon:10088 denotes mice
T1469 2172-2176 NCBITaxon:10088 denotes mice
T1470 2506-2510 NCBITaxon:10088 denotes mice
T1471 2657-2662 GO:0016265 denotes death
T1472 2715-2721 SO:0001023 denotes allele
T1473 2864-2870 SO:0001023 denotes allele
T1474 2902-2907 NCBITaxon:10088 denotes mouse
T1475 2982-2989 SO:0001026 denotes genomic
T1476 3014-3018 NCBITaxon:10088 denotes mice
T1477 3124-3128 NCBITaxon:10088 denotes mice
T7397 929-934 NCBITaxon:10088 denotes Mouse
T7398 941-945 SO:0000704 denotes Gene
T7399 973-989 SO:0001644 denotes targeting vector
T7400 1032-1036 SO:0000147 denotes exon
T7401 1044-1052 SO:0000357 denotes flanking
T7402 1184-1189 SO:0000147 denotes exons
T7403 1220-1226 SO:0001023 denotes allele
T7404 1273-1280 SO:0001026 denotes genomic
T7405 1290-1298 CL:0002322 denotes ES cells
T7406 1356-1371 SO:0000852 denotes portion of exon
T7407 1402-1418 SO:0001644 denotes targeting vector
T7408 1420-1430 GO:0097617 denotes hybridizes
T7409 1553-1560 CL:0002322 denotes ES cell
T7410 1603-1610 CL:0002322 denotes ES cell
T1443 0-4 SO:0000704 denotes Gene
T1444 48-52 NCBITaxon:10088 denotes Mice
T1445 68-74 SO:0000440 denotes vector
T1446 204-212 CL:0002322 denotes ES cells
T1447 285-290 SO:0000147 denotes exons
T1448 323-331 SO:0000357 denotes flanking
T1449 332-339 SO:0000155 denotes plasmid

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T1482 58-67 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T1484 76-80 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1485 110-120 GO_EXT:DNA_gap_repair denotes gap repair
T1486 133-134 CHEBI_SO_EXT:base denotes b
T1487 135-142 SO_EXT:sequence_deletion_process denotes deleted
T1488 166-179 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1489 204-212 CL:0002322 denotes ES cells
T1490 207-212 CL_GO_EXT:cell denotes cells
T1491 245-249 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1492 270-281 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T1493 285-290 SO_EXT:0000147 denotes exons
T1494 323-331 SO:0000357 denotes flanking
T1495 332-339 SO_EXT:0000155 denotes plasmid
T1496 344-347 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T1497 348-357 SO_EXT:biological_sequence denotes sequences
T1498 375-384 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T1499 385-391 SO_EXT:0000440 denotes vector
T1500 408-417 SO_EXT:sequence_duplication_process denotes duplicate
T1501 418-422 SO_EXT:0000147 denotes exon
T1502 443-447 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1503 455-466 GO:0006412 denotes Translation
T1504 474-484 SO_EXT:sequence_duplication_process denotes duplicated
T1505 485-489 SO_EXT:0000147 denotes exon
T1506 527-548 SO_EXT:0001587 denotes premature stop codons
T1507 562-572 SO_EXT:sequence_truncation_entity_or_process denotes truncation
T1508 580-584 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1509 618-638 SO_EXT:0001587 denotes premature stop codon
T1510 652-663 GO:0006412 denotes translation
T1511 678-689 CHEBI_SO_EXT:amino_acid denotes amino acids
T1512 699-709 SO_EXT:sequence_duplication_process denotes duplicated
T1513 710-714 SO_EXT:0000147 denotes exon
T1514 732-736 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1515 760-770 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T1516 771-777 SO_EXT:0000417 denotes domain
T1517 778-787 SO_EXT:0001117 denotes α-helixes
T1518 869-873 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1519 878-886 GO_EXT:tetrameric_macromolecular_complex denotes tetramer
T1534 1974-1978 CL_GO_EXT:cell denotes cell
T1535 1979-1985 SO_EXT:sequence_cloned_entity denotes clones
T1536 2060-2064 NCBITaxon:10088 denotes mice
T1537 2075-2079 NCBITaxon:10088 denotes mice
T1538 2125-2129 NCBITaxon:10088 denotes mice
T1539 2141-2145 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1540 2145-2146 SO_EXT:normal_or_wild_type_or_present denotes +
T1541 2147-2148 SO_EXT:sequence_nullness_or_absence denotes −
T1542 2164-2168 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1543 2168-2169 SO_EXT:sequence_nullness_or_absence denotes −
T1544 2170-2171 SO_EXT:sequence_nullness_or_absence denotes −
T1545 2172-2176 NCBITaxon:10088 denotes mice
T1546 2390-2397 SO_EXT:sequence_altered_entity denotes mutants
T1547 2417-2425 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T1548 2440-2446 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1549 2506-2510 NCBITaxon:10088 denotes mice
T1550 2521-2529 GO_EXT:breeder denotes breeders
T1551 2533-2543 SO_EXT:wild_type_entity_or_quality denotes wild-types
T1552 2570-2575 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T1553 2576-2584 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T1554 2648-2656 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T1555 2657-2662 GO:0016265 denotes death
T1556 2679-2688 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T1557 2715-2721 SO_EXT:0001023 denotes allele
T1558 2789-2798 SO_EXT:genotype_or_entity_with_genotype denotes genotypes
T1559 2864-2870 SO_EXT:0001023 denotes allele
T1560 2902-2907 NCBITaxon:10088 denotes mouse
T1561 2912-2916 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1562 2916-2917 SO_EXT:sequence_nullness_or_absence denotes −
T1563 2918-2919 SO_EXT:sequence_nullness_or_absence denotes −
T1564 2923-2927 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1565 2927-2928 SO_EXT:normal_or_wild_type_or_present denotes +
T1566 2929-2930 SO_EXT:sequence_nullness_or_absence denotes −
T1567 2982-2993 SO_EXT:genomic_DNA denotes genomic DNA
T1568 2990-2993 CHEBI_SO_EXT:DNA denotes DNA
T1569 3006-3010 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1570 3010-3011 SO_EXT:sequence_nullness_or_absence denotes −
T1571 3012-3013 SO_EXT:sequence_nullness_or_absence denotes −
T1572 3014-3018 NCBITaxon:10088 denotes mice
T1573 3104-3108 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1574 3108-3109 SO_EXT:sequence_nullness_or_absence denotes −
T1575 3110-3111 SO_EXT:sequence_nullness_or_absence denotes −
T1576 3116-3120 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1577 3120-3121 SO_EXT:normal_or_wild_type_or_present denotes +
T1578 3122-3123 SO_EXT:normal_or_wild_type_or_present denotes +
T1579 3124-3128 NCBITaxon:10088 denotes mice
T7411 929-934 NCBITaxon:10088 denotes Mouse
T7412 935-940 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T7413 941-945 SO_EXT:0000704 denotes Gene
T7414 954-958 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7415 963-972 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T7416 973-989 SO_EXT:0001644 denotes targeting vector
T7417 997-1004 SO_EXT:sequence_deletion_process denotes deleted
T7418 1010-1011 CHEBI_SO_EXT:base denotes b
T7419 1032-1036 SO_EXT:0000147 denotes exon
T7420 1044-1052 SO:0000357 denotes flanking
T7421 1053-1062 SO_EXT:biological_sequence denotes sequences
T7422 1064-1068 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7423 1083-1093 GO_EXT:DNA_gap_repair denotes gap repair
T7424 1110-1123 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T7425 1142-1147 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T7426 1169-1180 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T7427 1184-1189 SO_EXT:0000147 denotes exons
T7428 1220-1226 SO_EXT:0001023 denotes allele
T7429 1273-1284 SO_EXT:genomic_DNA denotes genomic DNA
T7430 1281-1284 CHEBI_SO_EXT:DNA denotes DNA
T7431 1290-1298 CL:0002322 denotes ES cells
T7432 1293-1298 CL_GO_EXT:cell denotes cells
T7433 1316-1321 CHEBI_SO_EXT:molecular_probe denotes Probe
T7434 1327-1330 CHEBI_SO_EXT:DNA denotes DNA
T7435 1356-1371 SO_EXT:0000852 denotes portion of exon
T7436 1402-1418 SO_EXT:0001644 denotes targeting vector
T7437 1420-1430 GO:0097617 denotes hybridizes
T7438 1453-1454 CHEBI_SO_EXT:base denotes b
T7439 1485-1498 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T7440 1511-1512 CHEBI_SO_EXT:base denotes b
T7441 1543-1552 SO_EXT:wild_type_entity_or_quality denotes wild-type
T7442 1553-1560 CL:0002322 denotes ES cell
T7443 1556-1560 CL_GO_EXT:cell denotes cell
T7444 1603-1610 CL:0002322 denotes ES cell
T7445 1606-1610 CL_GO_EXT:cell denotes cell
T1478 0-4 SO_EXT:0000704 denotes Gene
T1479 33-37 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1480 48-52 NCBITaxon:10088 denotes Mice
T1481 53-57 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1483 68-74 SO_EXT:0000440 denotes vector
T1520 1618-1625 CL:0002322 denotes ES cell
T1521 1621-1625 CL_GO_EXT:cell denotes cell
T1522 1626-1632 SO_EXT:sequence_cloned_entity denotes clones
T1523 1743-1747 SO_EXT:0000147 denotes exon
T1524 1751-1756 CHEBI_SO_EXT:molecular_probe denotes probe
T1525 1758-1763 CHEBI_SO_EXT:molecular_probe denotes probe
T1526 1787-1803 SO_EXT:0001644 denotes targeting vector
T1527 1805-1815 GO:0097617 denotes hybridized
T1528 1827-1828 CHEBI_SO_EXT:base denotes b
T1529 1858-1859 CHEBI_SO_EXT:base denotes b
T1530 1896-1905 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T1531 1913-1919 SO_EXT:0000440 denotes vector
T1532 1927-1932 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T1533 1971-1978 CL:0002322 denotes ES cell