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mice were examined for gross and histologic abnormalities, including one male and one female MCAD−/− mouse 18-mo-old, one male and one female MCAD+/+ mouse 6-mo-old, two male and two female MCAD−/− mice 4-wk-old, and two male and two female MCAD+/+ mice 4-wk-old. Kidney, spleen, pancreas, liver, brain, heart, testicles, ovaries, and skeletal muscle were fixed by immersion in buffered 10% formalin, processed routinely for paraffin sectioning, sectioned at 5 μm, and stained with hematoxylin and eosin. Frozen liver sections were prepared using standard methods and sections were stained with Oil-red-O. Slides were examined without knowledge of genotype or age. Other mice were examined because of sporadic clinical disease. Those with visible cardiac enlargement were evaluated for cardiac lesions."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T7153","span":{"begin":505,"end":516},"obj":"CHEBI:51686"},{"id":"T7157","span":{"begin":809,"end":816},"obj":"UBERON:0000948"},{"id":"T7156","span":{"begin":770,"end":777},"obj":"UBERON:0000948"},{"id":"T7155","span":{"begin":694,"end":698},"obj":"NCBITaxon:10088"},{"id":"T7154","span":{"begin":535,"end":540},"obj":"UBERON:0002107"},{"id":"T7152","span":{"begin":414,"end":422},"obj":"CHEBI:16842"},{"id":"T7151","span":{"begin":358,"end":366},"obj":"UBERON:0004288"},{"id":"T7150","span":{"begin":345,"end":352},"obj":"UBERON:0000992"},{"id":"T7149","span":{"begin":334,"end":343},"obj":"UBERON:0000473"},{"id":"T7148","span":{"begin":327,"end":332},"obj":"UBERON:0000948"},{"id":"T7147","span":{"begin":320,"end":325},"obj":"UBERON:0000955"},{"id":"T7146","span":{"begin":313,"end":318},"obj":"UBERON:0002107"},{"id":"T7145","span":{"begin":303,"end":311},"obj":"UBERON:0001264"},{"id":"T7144","span":{"begin":295,"end":301},"obj":"UBERON:0002106"},{"id":"T7143","span":{"begin":287,"end":293},"obj":"UBERON:0002113"},{"id":"T7142","span":{"begin":272,"end":276},"obj":"NCBITaxon:10088"},{"id":"T7141","span":{"begin":221,"end":225},"obj":"NCBITaxon:10088"},{"id":"T7140","span":{"begin":173,"end":178},"obj":"NCBITaxon:10088"},{"id":"T7139","span":{"begin":124,"end":129},"obj":"NCBITaxon:10088"},{"id":"T7138","span":{"begin":23,"end":27},"obj":"NCBITaxon:10088"}],"text":"Histopathology.\nTwelve mice were examined for gross and histologic abnormalities, including one male and one female MCAD−/− mouse 18-mo-old, one male and one female MCAD+/+ mouse 6-mo-old, two male and two female MCAD−/− mice 4-wk-old, and two male and two female MCAD+/+ mice 4-wk-old. Kidney, spleen, pancreas, liver, brain, heart, testicles, ovaries, and skeletal muscle were fixed by immersion in buffered 10% formalin, processed routinely for paraffin sectioning, sectioned at 5 μm, and stained with hematoxylin and eosin. Frozen liver sections were prepared using standard methods and sections were stained with Oil-red-O. Slides were examined without knowledge of genotype or age. Other mice were examined because of sporadic clinical disease. Those with visible cardiac enlargement were evaluated for cardiac lesions."}

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