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PMC:1189074 / 24909-26133 JSONTXT

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Id Subject Object Predicate Lexical cue
T6488 1223-1224 . denotes .
T6487 1222-1223 -RRB- denotes )
T6486 1216-1222 NNP denotes States
T6485 1209-1215 NNP denotes United
T6484 1207-1209 , denotes ,
T6483 1194-1207 NNP denotes Massachusetts
T6482 1192-1194 , denotes ,
T6481 1186-1192 NNP denotes Boston
T6480 1184-1186 , denotes ,
T6479 1163-1175 NNP denotes Lifesciences
T6478 1176-1184 NNP denotes Products
T6477 1159-1162 NNP denotes NEN
T6476 1157-1159 , denotes ,
T6475 1146-1157 NNP denotes Renaissance
T6474 1145-1146 -LRB- denotes (
T6473 1127-1144 NN denotes chemiluminiscence
T6472 1124-1126 IN denotes by
T6471 1114-1123 VBN denotes developed
T6470 1110-1113 CC denotes and
T6469 1099-1109 NNS denotes procedures
T6468 1090-1098 JJ denotes standard
T6467 1084-1089 VBG denotes using
T6466 1072-1083 NN denotes temperature
T6465 1067-1071 NN denotes room
T6464 1064-1066 IN denotes at
T6463 1062-1063 NN denotes h
T6462 1059-1060 SYM denotes –
T6461 1060-1061 CD denotes 4
T6460 1058-1059 CD denotes 2
T6459 1054-1057 IN denotes for
T6458 1035-1044 JJ denotes secondary
T6457 1023-1024 HYPH denotes -
T6456 1024-1034 VBN denotes conjugated
T6455 1020-1023 NN denotes HRP
T6454 1016-1019 NN denotes IgG
T6453 1045-1053 NN denotes antibody
T6452 1005-1015 JJ denotes anti-mouse
T6451 1002-1004 IN denotes in
T6450 987-991 VBD denotes were
T6449 992-1001 VBN denotes incubated
T6448 981-986 NNS denotes Blots
T6447 980-1224 sentence denotes Blots were incubated in anti-mouse IgG HRP-conjugated secondary antibody for 2–4 h at room temperature using standard procedures and developed by chemiluminiscence (Renaissance, NEN Lifesciences Products, Boston, Massachusetts, United States).
T6446 979-980 . denotes .
T6445 961-970 JJ denotes anti-MCAD
T6444 971-979 NN denotes antibody
T6443 958-960 DT denotes an
T6442 955-957 IN denotes of
T6441 946-954 NN denotes dilution
T6440 941-942 SYM denotes :
T6439 942-945 CD denotes 500
T6438 940-941 CD denotes 1
T6437 935-939 IN denotes with
T6436 932-934 NN denotes °C
T6435 930-931 CD denotes 4
T6434 927-929 IN denotes at
T6433 917-926 RB denotes overnight
T6432 899-902 VBD denotes was
T6431 890-898 NN denotes membrane
T6430 886-889 DT denotes the
T6429 884-886 , denotes ,
T6428 882-884 CD denotes 20
T6427 881-882 HYPH denotes -
T6426 876-881 NN denotes Tween
T6425 874-875 NN denotes %
T6424 871-874 CD denotes 0.1
T6423 866-870 IN denotes with
T6359 568-569 -LRB- denotes (
T6358 554-561 NN denotes protein
T6357 550-553 NN denotes BCA
T6356 562-567 NN denotes assay
T6355 541-549 NNP denotes Bradford
T6354 538-540 IN denotes by
T6353 522-526 VBD denotes were
T6352 527-537 VBN denotes quantified
T6351 514-521 NNS denotes Lysates
T6350 513-615 sentence denotes Lysates were quantified by Bradford BCA protein assay (Bio-Rad, Hercules, California, United States).
T6349 512-513 . denotes .
T6348 499-512 NN denotes orthovanadate
T6347 492-498 NN denotes sodium
T6346 489-491 IN denotes of
T6345 486-488 NN denotes mM
T6344 484-485 CD denotes 1
T6343 480-483 CC denotes and
T6342 478-480 , denotes ,
T6341 450-478 NN denotes phenylmethylsulfonylfluoride
T6340 447-449 IN denotes of
T6339 444-446 NN denotes mM
T6338 442-443 CD denotes 1
T6337 440-442 , denotes ,
T6336 439-440 -RRB- denotes )
T6335 433-439 NNP denotes States
T6334 426-432 NNP denotes United
T6333 424-426 , denotes ,
T6332 417-424 NNP denotes Indiana
T6331 415-417 , denotes ,
T6330 403-415 NNP denotes Indianapolis
T6329 401-403 , denotes ,
T6328 378-389 NNP denotes Diagnostics
T6327 372-377 NNP denotes Roche
T6326 390-401 NNP denotes Corporation
T6325 371-372 -LRB- denotes (
T6324 369-371 , denotes ,
T6323 351-359 NN denotes Protease
T6322 360-369 NN denotes Inhibitor
T6321 342-350 JJ denotes Complete
T6320 338-341 CC denotes and
T6319 329-337 NN denotes glycerol
T6318 327-328 NN denotes %
T6317 325-327 CD denotes 10
T6316 320-324 IN denotes with
T6315 318-319 -RRB- denotes )
T6314 315-318 NN denotes SDS
T6313 313-314 NN denotes %
T6312 312-313 CD denotes 1
T6311 308-311 CC denotes and
T6310 306-308 , denotes ,
T6309 287-293 NN denotes sodium
T6308 294-306 NN denotes deoxycholate
T6307 285-286 NN denotes %
T6306 282-285 CD denotes 0.5
T6305 280-282 , denotes ,
T6304 278-280 CD denotes 40
T6303 277-278 HYPH denotes -
T6302 268-275 NN denotes Nonidet
T6301 276-277 NN denotes P
T6300 266-267 NN denotes %
T6299 265-266 CD denotes 1
T6298 263-265 , denotes ,
T6297 258-259 SYM denotes ×
T6296 257-258 CD denotes 1
T6295 260-263 NN denotes PBS
T6294 256-257 -LRB- denotes (
T6293 249-255 NN denotes buffer
T6292 244-248 NN denotes RIPA
T6291 241-243 IN denotes in
T6290 235-240 VBN denotes lysed
T6289 231-234 CC denotes and
T6288 215-218 VBD denotes was
T6287 208-214 NN denotes tissue
T6286 206-208 , denotes ,
T6285 198-206 NN denotes analysis
T6284 219-230 VBN denotes homogenized
T6283 194-197 IN denotes For
T6282 193-513 sentence denotes For analysis, tissue was homogenized and lysed in RIPA buffer (1× PBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, and 1% SDS) with 10% glycerol and Complete Protease Inhibitor, (Roche Diagnostics Corporation, Indianapolis, Indiana, United States), 1 mM of phenylmethylsulfonylfluoride, and 1 mM of sodium orthovanadate.
T6281 192-193 . denotes .
T6280 190-192 NN denotes °C
T6279 187-189 CD denotes 80
T6278 186-187 SYM denotes −
T6277 183-185 IN denotes at
T6276 176-182 VBN denotes stored
T6275 172-175 CC denotes and
T6274 163-171 NN denotes nitrogen
T6273 156-162 JJ denotes liquid
T6272 153-155 IN denotes in
T6271 134-145 RB denotes immediately
T6270 129-133 VBD denotes were
T6269 122-123 -RRB- denotes )
T6268 119-120 SYM denotes =
T6267 117-118 NN denotes n
T6266 121-122 CD denotes 3
T6265 116-117 -LRB- denotes (
T6264 114-115 SYM denotes −
T6263 113-114 HYPH denotes /
T6262 112-113 SYM denotes −
T6261 108-112 NN denotes MCAD
T6260 104-107 CC denotes and
T6259 102-103 -RRB- denotes )
T6258 99-100 SYM denotes =
T6257 97-98 NN denotes n
T6256 101-102 CD denotes 1
T6255 96-97 -LRB- denotes (
T6254 94-95 SYM denotes +
T6253 93-94 HYPH denotes /
T6252 92-93 SYM denotes +
T6251 88-92 NN denotes MCAD
T6250 124-128 NNS denotes mice
T6249 83-84 HYPH denotes -
T6248 84-87 JJ denotes old
T6247 80-81 HYPH denotes -
T6246 81-83 NN denotes wk
T6245 78-79 SYM denotes –
T6244 79-80 CD denotes 8
T6243 77-78 CD denotes 6
T6242 72-76 IN denotes from
T6241 64-71 NNS denotes samples
T6240 58-63 NN denotes liver
T6239 56-58 , denotes ,
T6238 50-56 NN denotes tissue
T6237 44-49 NN denotes mouse
T6236 41-43 IN denotes in
T6235 33-40 NN denotes protein
T6234 28-32 NN denotes MCAD
T6233 25-27 IN denotes of
T6232 16-24 NN denotes quantity
T6231 12-15 DT denotes the
T6230 146-152 VBN denotes frozen
T6229 3-11 VB denotes evaluate
T6228 0-2 TO denotes To
T6422 859-865 NN denotes saline
T6421 849-850 HYPH denotes -
T6420 850-858 VBN denotes buffered
T6419 840-849 NN denotes phosphate
T6418 837-839 IN denotes in
T6417 825-831 JJ denotes nonfat
T6416 832-836 NN denotes milk
T6415 823-824 NN denotes %
T6414 822-823 CD denotes 5
T6413 817-821 IN denotes with
T6412 808-816 VBG denotes blocking
T6411 903-916 VBN denotes immunoblotted
T6410 802-807 IN denotes After
T6409 801-980 sentence denotes After blocking with 5% nonfat milk in phosphate-buffered saline with 0.1% Tween-20, the membrane was immunoblotted overnight at 4 °C with 1:500 dilution of an anti-MCAD antibody.
T6408 800-801 . denotes .
T6407 799-800 -RRB- denotes )
T6406 793-799 NNP denotes States
T6405 786-792 NNP denotes United
T6404 784-786 , denotes ,
T6403 775-784 NNP denotes Hampshire
T6402 771-774 NNP denotes New
T6401 769-771 , denotes ,
T6400 764-769 NNP denotes Keene
T6399 762-764 , denotes ,
T6398 755-762 NNP denotes Schuell
T6397 751-754 CC denotes and
T6396 740-750 NNP denotes Schleicher
T6395 739-740 -LRB- denotes (
T6394 715-729 NN denotes nitrocellulose
T6393 712-714 NN denotes μm
T6392 707-711 CD denotes 0.45
T6391 730-738 NN denotes membrane
T6390 705-706 DT denotes a
T6389 700-704 IN denotes onto
T6388 690-699 RB denotes overnight
T6387 678-689 VBN denotes transferred
T6386 674-677 CC denotes and
T6385 672-674 , denotes ,
T6384 667-668 HYPH denotes -
T6383 664-667 NN denotes SDS
T6382 668-672 NN denotes PAGE
T6381 662-663 NN denotes %
T6380 661-662 CD denotes 8
T6379 658-660 IN denotes in
T6378 648-657 VBN denotes separated
T6377 646-648 , denotes ,
T6376 632-636 VBD denotes were
T6375 637-646 VBN denotes denatured
T6374 624-631 NNS denotes lysates
T6373 616-623 NN denotes Protein
T6372 615-801 sentence denotes Protein lysates were denatured, separated in 8% SDS-PAGE, and transferred overnight onto a 0.45 μm nitrocellulose membrane (Schleicher and Schuell, Keene, New Hampshire, United States).
T6371 614-615 . denotes .
T6370 613-614 -RRB- denotes )
T6369 607-613 NNP denotes States
T6368 600-606 NNP denotes United
T6367 598-600 , denotes ,
T6366 588-598 NNP denotes California
T6365 586-588 , denotes ,
T6364 578-586 NNP denotes Hercules
T6363 576-578 , denotes ,
T6362 572-573 HYPH denotes -
T6361 569-572 NNP denotes Bio
T6360 573-576 NNP denotes Rad
R3950 T6228 T6229 aux To,evaluate
R3951 T6229 T6230 advcl evaluate,frozen
R3952 T6231 T6232 det the,quantity
R3953 T6232 T6229 dobj quantity,evaluate
R3954 T6233 T6232 prep of,quantity
R3955 T6234 T6235 compound MCAD,protein
R3956 T6235 T6233 pobj protein,of
R3957 T6236 T6229 prep in,evaluate
R3958 T6237 T6238 compound mouse,tissue
R3959 T6238 T6236 pobj tissue,in
R3960 T6239 T6230 punct ", ",frozen
R3961 T6240 T6241 compound liver,samples
R3962 T6241 T6230 nsubjpass samples,frozen
R3963 T6242 T6241 prep from,samples
R3964 T6243 T6244 quantmod 6,8
R3965 T6244 T6246 nummod 8,wk
R3966 T6245 T6244 punct –,8
R3967 T6246 T6248 npadvmod wk,old
R3968 T6247 T6246 punct -,wk
R3969 T6248 T6250 amod old,mice
R3970 T6249 T6248 punct -,old
R3971 T6250 T6242 pobj mice,from
R3972 T6251 T6250 nmod MCAD,mice
R3973 T6252 T6251 punct +,MCAD
R3974 T6253 T6251 punct /,MCAD
R3975 T6254 T6251 punct +,MCAD
R3976 T6255 T6256 punct (,1
R3977 T6256 T6251 parataxis 1,MCAD
R3978 T6257 T6256 nsubj n,1
R3979 T6258 T6256 punct =,1
R3980 T6259 T6256 punct ),1
R3981 T6260 T6251 cc and,MCAD
R3982 T6261 T6251 conj MCAD,MCAD
R3983 T6262 T6261 punct −,MCAD
R3984 T6263 T6261 punct /,MCAD
R3985 T6264 T6261 punct −,MCAD
R3986 T6265 T6266 punct (,3
R3987 T6266 T6261 parataxis 3,MCAD
R3988 T6267 T6266 nsubj n,3
R3989 T6268 T6266 punct =,3
R3990 T6269 T6266 punct ),3
R3991 T6270 T6230 auxpass were,frozen
R3992 T6271 T6230 advmod immediately,frozen
R3993 T6272 T6230 prep in,frozen
R3994 T6273 T6274 amod liquid,nitrogen
R3995 T6274 T6272 pobj nitrogen,in
R3996 T6275 T6230 cc and,frozen
R3997 T6276 T6230 conj stored,frozen
R3998 T6277 T6276 prep at,stored
R3999 T6278 T6279 punct −,80
R4000 T6279 T6280 nummod 80,°C
R4001 T6280 T6277 pobj °C,at
R4002 T6281 T6230 punct .,frozen
R4003 T6283 T6284 prep For,homogenized
R4004 T6285 T6283 pobj analysis,For
R4005 T6286 T6284 punct ", ",homogenized
R4006 T6287 T6284 nsubjpass tissue,homogenized
R4007 T6288 T6284 auxpass was,homogenized
R4008 T6289 T6284 cc and,homogenized
R4009 T6290 T6284 conj lysed,homogenized
R4010 T6291 T6290 prep in,lysed
R4011 T6292 T6293 compound RIPA,buffer
R4012 T6293 T6291 pobj buffer,in
R4013 T6294 T6295 punct (,PBS
R4014 T6295 T6293 parataxis PBS,buffer
R4015 T6296 T6295 nummod 1,PBS
R4016 T6297 T6296 punct ×,1
R4017 T6298 T6295 punct ", ",PBS
R4018 T6299 T6300 nummod 1,%
R4019 T6300 T6301 compound %,P
R4020 T6301 T6295 conj P,PBS
R4021 T6302 T6301 compound Nonidet,P
R4022 T6303 T6301 punct -,P
R4023 T6304 T6301 nummod 40,P
R4024 T6305 T6301 punct ", ",P
R4025 T6306 T6307 nummod 0.5,%
R4026 T6307 T6308 compound %,deoxycholate
R4027 T6308 T6301 conj deoxycholate,P
R4028 T6309 T6308 compound sodium,deoxycholate
R4029 T6310 T6308 punct ", ",deoxycholate
R4030 T6311 T6308 cc and,deoxycholate
R4031 T6312 T6313 nummod 1,%
R4032 T6313 T6314 compound %,SDS
R4033 T6314 T6308 conj SDS,deoxycholate
R4034 T6315 T6295 punct ),PBS
R4035 T6316 T6290 prep with,lysed
R4036 T6317 T6318 nummod 10,%
R4037 T6318 T6319 compound %,glycerol
R4038 T6319 T6316 pobj glycerol,with
R4039 T6320 T6319 cc and,glycerol
R4040 T6321 T6322 amod Complete,Inhibitor
R4041 T6322 T6319 conj Inhibitor,glycerol
R4042 T6323 T6322 compound Protease,Inhibitor
R4043 T6324 T6322 punct ", ",Inhibitor
R4044 T6325 T6326 punct (,Corporation
R4045 T6326 T6322 conj Corporation,Inhibitor
R4046 T6327 T6326 compound Roche,Corporation
R4047 T6328 T6326 compound Diagnostics,Corporation
R4048 T6329 T6326 punct ", ",Corporation
R4049 T6330 T6326 npadvmod Indianapolis,Corporation
R4050 T6331 T6326 punct ", ",Corporation
R4051 T6332 T6326 npadvmod Indiana,Corporation
R4052 T6333 T6326 punct ", ",Corporation
R4053 T6334 T6335 compound United,States
R4054 T6335 T6326 npadvmod States,Corporation
R4055 T6336 T6326 punct ),Corporation
R4056 T6337 T6319 punct ", ",glycerol
R4057 T6338 T6339 nummod 1,mM
R4058 T6339 T6319 conj mM,glycerol
R4059 T6340 T6339 prep of,mM
R4060 T6341 T6340 pobj phenylmethylsulfonylfluoride,of
R4061 T6342 T6339 punct ", ",mM
R4062 T6343 T6339 cc and,mM
R4063 T6344 T6345 nummod 1,mM
R4064 T6345 T6339 conj mM,mM
R4065 T6346 T6345 prep of,mM
R4066 T6347 T6348 compound sodium,orthovanadate
R4067 T6348 T6346 pobj orthovanadate,of
R4068 T6349 T6284 punct .,homogenized
R4069 T6351 T6352 nsubjpass Lysates,quantified
R4070 T6353 T6352 auxpass were,quantified
R4071 T6354 T6352 prep by,quantified
R4072 T6355 T6356 compound Bradford,assay
R4073 T6356 T6354 pobj assay,by
R4074 T6357 T6356 compound BCA,assay
R4075 T6358 T6356 compound protein,assay
R4076 T6359 T6360 punct (,Rad
R4077 T6360 T6356 parataxis Rad,assay
R4078 T6361 T6360 compound Bio,Rad
R4079 T6362 T6360 punct -,Rad
R4080 T6363 T6360 punct ", ",Rad
R4081 T6364 T6360 npadvmod Hercules,Rad
R4082 T6365 T6360 punct ", ",Rad
R4083 T6366 T6360 npadvmod California,Rad
R4084 T6367 T6360 punct ", ",Rad
R4085 T6368 T6369 compound United,States
R4086 T6369 T6360 npadvmod States,Rad
R4087 T6370 T6360 punct ),Rad
R4088 T6371 T6352 punct .,quantified
R4089 T6373 T6374 compound Protein,lysates
R4090 T6374 T6375 nsubjpass lysates,denatured
R4091 T6376 T6375 auxpass were,denatured
R4092 T6377 T6375 punct ", ",denatured
R4093 T6378 T6375 conj separated,denatured
R4094 T6379 T6378 prep in,separated
R4095 T6380 T6381 nummod 8,%
R4096 T6381 T6382 compound %,PAGE
R4097 T6382 T6379 pobj PAGE,in
R4098 T6383 T6382 compound SDS,PAGE
R4099 T6384 T6382 punct -,PAGE
R4100 T6385 T6378 punct ", ",separated
R4101 T6386 T6378 cc and,separated
R4102 T6387 T6378 conj transferred,separated
R4103 T6388 T6387 advmod overnight,transferred
R4104 T6389 T6387 prep onto,transferred
R4105 T6390 T6391 det a,membrane
R4106 T6391 T6389 pobj membrane,onto
R4107 T6392 T6393 nummod 0.45,μm
R4108 T6393 T6391 compound μm,membrane
R4109 T6394 T6391 compound nitrocellulose,membrane
R4110 T6395 T6396 punct (,Schleicher
R4111 T6396 T6391 parataxis Schleicher,membrane
R4112 T6397 T6396 cc and,Schleicher
R4113 T6398 T6396 conj Schuell,Schleicher
R4114 T6399 T6396 punct ", ",Schleicher
R4115 T6400 T6396 npadvmod Keene,Schleicher
R4116 T6401 T6396 punct ", ",Schleicher
R4117 T6402 T6403 compound New,Hampshire
R4118 T6403 T6396 npadvmod Hampshire,Schleicher
R4119 T6404 T6396 punct ", ",Schleicher
R4120 T6405 T6406 compound United,States
R4121 T6406 T6396 npadvmod States,Schleicher
R4122 T6407 T6396 punct ),Schleicher
R4123 T6408 T6375 punct .,denatured
R4124 T6410 T6411 prep After,immunoblotted
R4125 T6412 T6410 pcomp blocking,After
R4126 T6413 T6412 prep with,blocking
R4127 T6414 T6415 nummod 5,%
R4128 T6415 T6416 nmod %,milk
R4129 T6416 T6413 pobj milk,with
R4130 T6417 T6416 amod nonfat,milk
R4131 T6418 T6412 prep in,blocking
R4132 T6419 T6420 npadvmod phosphate,buffered
R4133 T6420 T6422 amod buffered,saline
R4134 T6421 T6420 punct -,buffered
R4135 T6422 T6418 pobj saline,in
R4136 T6423 T6422 prep with,saline
R4137 T6424 T6425 nummod 0.1,%
R4138 T6425 T6426 compound %,Tween
R4139 T6426 T6423 pobj Tween,with
R4140 T6427 T6426 punct -,Tween
R4141 T6428 T6426 nummod 20,Tween
R4142 T6429 T6411 punct ", ",immunoblotted
R4143 T6430 T6431 det the,membrane
R4144 T6431 T6411 nsubjpass membrane,immunoblotted
R4145 T6432 T6411 auxpass was,immunoblotted
R4146 T6433 T6411 advmod overnight,immunoblotted
R4147 T6434 T6411 prep at,immunoblotted
R4148 T6435 T6436 nummod 4,°C
R4149 T6436 T6434 pobj °C,at
R4150 T6437 T6411 prep with,immunoblotted
R4151 T6438 T6439 quantmod 1,500
R4152 T6439 T6441 nummod 500,dilution
R4153 T6440 T6439 punct :,500
R4154 T6441 T6437 pobj dilution,with
R4155 T6442 T6441 prep of,dilution
R4156 T6443 T6444 det an,antibody
R4157 T6444 T6442 pobj antibody,of
R4158 T6445 T6444 amod anti-MCAD,antibody
R4159 T6446 T6411 punct .,immunoblotted
R4160 T6448 T6449 nsubjpass Blots,incubated
R4161 T6450 T6449 auxpass were,incubated
R4162 T6451 T6449 prep in,incubated
R4163 T6452 T6453 amod anti-mouse,antibody
R4164 T6453 T6451 pobj antibody,in
R4165 T6454 T6453 nmod IgG,antibody
R4166 T6455 T6456 npadvmod HRP,conjugated
R4167 T6456 T6453 amod conjugated,antibody
R4168 T6457 T6456 punct -,conjugated
R4169 T6458 T6453 amod secondary,antibody
R4170 T6459 T6449 prep for,incubated
R4171 T6460 T6461 quantmod 2,4
R4172 T6461 T6463 nummod 4,h
R4173 T6462 T6461 punct –,4
R4174 T6463 T6459 pobj h,for
R4175 T6464 T6449 prep at,incubated
R4176 T6465 T6466 compound room,temperature
R4177 T6466 T6464 pobj temperature,at
R4178 T6467 T6449 advcl using,incubated
R4179 T6468 T6469 amod standard,procedures
R4180 T6469 T6467 dobj procedures,using
R4181 T6470 T6449 cc and,incubated
R4182 T6471 T6449 conj developed,incubated
R4183 T6472 T6471 prep by,developed
R4184 T6473 T6472 pobj chemiluminiscence,by
R4185 T6474 T6475 punct (,Renaissance
R4186 T6475 T6473 parataxis Renaissance,chemiluminiscence
R4187 T6476 T6475 punct ", ",Renaissance
R4188 T6477 T6478 compound NEN,Products
R4189 T6478 T6475 appos Products,Renaissance
R4190 T6479 T6478 compound Lifesciences,Products
R4191 T6480 T6475 punct ", ",Renaissance
R4192 T6481 T6475 npadvmod Boston,Renaissance
R4193 T6482 T6475 punct ", ",Renaissance
R4194 T6483 T6475 npadvmod Massachusetts,Renaissance
R4195 T6484 T6475 punct ", ",Renaissance
R4196 T6485 T6486 compound United,States
R4197 T6486 T6475 npadvmod States,Renaissance
R4198 T6487 T6475 punct ),Renaissance
R4199 T6488 T6449 punct .,incubated

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T6509 1045-1053 GO:0042571 denotes antibody
T6508 1024-1034 MOP:0000779 denotes conjugated
T6507 1016-1019 GO:0071735 denotes IgG
T6506 1010-1015 NCBITaxon:10088 denotes mouse
T6505 971-979 GO:0042571 denotes antibody
T6504 876-884 CHEBI:53424 denotes Tween-20
T6503 832-836 UBERON:0001913 denotes milk
T6502 715-729 CHEBI:53325 denotes nitrocellulose
T6501 664-667 CHEBI:8984 denotes SDS
T6500 492-512 CHEBI:35607 denotes sodium orthovanadate
T6499 450-478 CHEBI:8102 denotes phenylmethylsulfonylfluoride
T6498 329-337 CHEBI:17754 denotes glycerol
T6497 315-318 CHEBI:8984 denotes SDS
T6496 287-306 CHEBI:9177 denotes sodium deoxycholate
T6495 268-280 CHEBI:78708 denotes Nonidet P-40
T6494 235-240 GO:0019835 denotes lysed
T6493 208-214 UBERON:0000479 denotes tissue
T6492 124-128 NCBITaxon:10088 denotes mice
T6491 58-63 UBERON:0002107 denotes liver
T6490 50-56 UBERON:0000479 denotes tissue
T6489 44-49 NCBITaxon:10088 denotes mouse

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T6552 1045-1053 GO:0042571 denotes antibody
T6551 1024-1034 MOP:0000779 denotes conjugated
T6550 1016-1019 GO:0071735 denotes IgG
T6549 1010-1015 NCBITaxon:10088 denotes mouse
T6548 971-979 GO:0042571 denotes antibody
T6547 966-970 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6546 876-884 CHEBI:53424 denotes Tween-20
T6545 859-865 CHEBI_EXT:saline_solution denotes saline
T6544 850-858 CHEBI_CHMO_EXT:buffer_process denotes buffered
T6543 840-849 CHEBI_EXT:phosphate denotes phosphate
T6542 832-836 UBERON:0001913 denotes milk
T6541 828-831 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T6540 715-729 CHEBI:53325 denotes nitrocellulose
T6539 678-689 GO_EXT:biological_movement_or_translocation_process denotes transferred
T6538 664-667 CHEBI:8984 denotes SDS
T6537 637-646 GO_EXT:macromolecule_denaturation denotes denatured
T6536 616-623 CHEBI_PR_EXT:protein denotes Protein
T6535 554-561 CHEBI_PR_EXT:protein denotes protein
T6534 492-512 CHEBI:35607 denotes sodium orthovanadate
T6533 450-478 CHEBI:8102 denotes phenylmethylsulfonylfluoride
T6532 351-369 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes Protease Inhibitor
T6531 329-337 CHEBI:17754 denotes glycerol
T6530 315-318 CHEBI:8984 denotes SDS
T6529 287-306 CHEBI:9177 denotes sodium deoxycholate
T6528 268-280 CHEBI:78708 denotes Nonidet P-40
T6527 249-255 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T6526 235-240 GO:0019835 denotes lysed
T6525 208-214 UBERON:0000479 denotes tissue
T6524 163-171 CHEBI_EXT:nitrogen denotes nitrogen
T6523 124-128 NCBITaxon:10088 denotes mice
T6522 114-115 SO_EXT:sequence_nullness_or_absence denotes −
T6521 112-113 SO_EXT:sequence_nullness_or_absence denotes −
T6520 108-112 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6519 94-95 SO_EXT:normal_or_wild_type_or_present denotes +
T6518 92-93 SO_EXT:normal_or_wild_type_or_present denotes +
T6517 88-92 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6516 58-63 UBERON:0002107 denotes liver
T6515 50-56 UBERON:0000479 denotes tissue
T6514 44-49 NCBITaxon:10088 denotes mouse
T6513 33-40 CHEBI_PR_EXT:protein denotes protein
T6512 28-32 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD