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PMC:1189074 / 20587-21690 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T5173 1076-1080 NN denotes exon
T5172 1072-1073 HYPH denotes -
T5171 1073-1075 NN denotes kb
T5170 1069-1072 CD denotes 1.3
T5169 1084-1090 NN denotes region
T5168 1065-1068 DT denotes the
T5167 1095-1102 VBN denotes deleted
T5166 1059-1064 WRB denotes where
T5165 1049-1053 NN denotes SalI
T5164 1042-1048 JJ denotes unique
T5163 1054-1058 NN denotes site
T5162 1040-1041 DT denotes a
T5161 1028-1030 , denotes ,
T5160 1020-1028 NN denotes homology
T5159 1017-1019 IN denotes of
T5158 1007-1012 JJ denotes short
T5157 1013-1016 NN denotes arm
T5156 1003-1006 DT denotes the
T5155 1000-1002 IN denotes to
T5154 991-995 JJ denotes long
T5153 996-999 NN denotes arm
T5152 987-990 DT denotes the
T5151 984-986 IN denotes of
T5150 975-983 NN denotes ligation
T5149 971-974 DT denotes the
T5148 966-970 IN denotes from
T5147 956-965 VBG denotes resulting
T5146 954-956 , denotes ,
T5145 1030-1039 VBD denotes contained
T5144 933-947 VBN denotes recircularized
T5143 948-954 NN denotes vector
T5142 929-932 DT denotes The
T5141 928-1103 sentence denotes The recircularized vector, resulting from the ligation of the long arm to the short arm of homology, contained a unique SalI site where the 1.3-kb exon 10 region was deleted.
T5140 927-928 . denotes .
T5139 926-927 SYM denotes ′
T5138 925-926 CD denotes 3
T5137 924-925 HYPH denotes -
T5136 912-913 HYPH denotes -
T5135 911-912 SYM denotes ′
T5134 913-924 NN denotes TCGATGTCGAC
T5133 910-911 CD denotes 5
T5132 906-909 CC denotes and
T5131 904-905 : denotes ;
T5130 903-904 SYM denotes ′
T5129 902-903 CD denotes 3
T5128 901-902 HYPH denotes -
T5127 889-890 SYM denotes ′
T5126 890-901 NN denotes GATCGTCGACA
T5125 888-889 CD denotes 5
T5124 886-887 : denotes ;
T5123 885-886 SYM denotes ′
T5122 884-885 CD denotes 3
T5121 883-884 HYPH denotes -
T5120 871-872 HYPH denotes -
T5119 870-871 SYM denotes ′
T5118 872-883 NN denotes AATTGTCGACA
T5117 869-870 CD denotes 5
T5116 867-869 : denotes :
T5115 851-867 NNS denotes oligonucleotides
T5114 845-850 CD denotes three
T5113 839-844 VBG denotes using
T5112 830-838 NN denotes ligation
T5111 827-829 IN denotes by
T5110 812-826 VBN denotes recircularized
T5109 808-811 CC denotes and
T5108 795-807 NN denotes purification
T5107 791-794 NN denotes gel
T5106 788-790 IN denotes by
T5105 775-778 VBD denotes was
T5104 773-775 , denotes ,
T5103 757-764 JJ denotes genomic
T5102 750-751 HYPH denotes /
T5101 751-756 NN denotes EcoRI
T5100 745-750 NN denotes BamHI
T5099 741-742 HYPH denotes -
T5098 742-744 NN denotes kb
T5097 738-741 CD denotes 1.3
T5096 765-773 NN denotes fragment
T5095 734-737 DT denotes the
T5094 726-733 IN denotes without
T5093 724-726 , denotes ,
T5092 779-787 VBN denotes purified
T5091 709-717 VBN denotes digested
T5090 718-724 NN denotes vector
T5089 705-708 DT denotes The
T5088 704-928 sentence denotes The digested vector, without the 1.3-kb BamHI/EcoRI genomic fragment, was purified by gel purification and recircularized by ligation using three oligonucleotides: 5′-AATTGTCGACA-3′; 5′GATCGTCGACA-3′; and 5′-TCGATGTCGAC-3′.
T5087 703-704 . denotes .
T5086 694-703 NNS denotes sequences
T5085 687-693 NN denotes intron
T5084 678-686 VBG denotes flanking
T5083 674-677 CC denotes and
T5082 671-673 CD denotes 10
T5081 666-670 NN denotes exon
T5080 655-665 VBG denotes containing
T5079 638-645 JJ denotes genomic
T5078 631-632 HYPH denotes /
T5077 632-637 NN denotes EcoRI
T5076 626-631 NN denotes BamHI
T5075 622-623 HYPH denotes -
T5074 623-625 NN denotes kb
T5073 619-622 CD denotes 1.3
T5072 646-654 NN denotes fragment
T5071 617-618 DT denotes a
T5070 610-616 VB denotes remove
T5069 607-609 TO denotes to
T5068 601-606 NN denotes EcoRI
T5067 597-600 CC denotes and
T5066 591-596 NN denotes BamHI
T5065 586-590 IN denotes with
T5064 573-576 VBD denotes was
T5063 577-585 VBN denotes digested
T5062 566-572 NN denotes vector
T5061 562-565 DT denotes The
T5060 561-704 sentence denotes The vector was digested with BamHI and EcoRI to remove a 1.3-kb BamHI/EcoRI genomic fragment containing exon 10 and flanking intron sequences.
T5059 560-561 . denotes .
T5058 559-560 -RRB- denotes )
T5057 558-559 SYM denotes +
T5056 557-558 -LRB- denotes (
T5055 549-557 NN denotes pGEM11Zf
T5054 546-548 IN denotes of
T5053 535-539 NN denotes XhoI
T5052 531-534 CC denotes and
T5051 526-530 NN denotes NotI
T5050 540-545 NNS denotes sites
T5049 522-525 DT denotes the
T5048 517-521 IN denotes into
T5047 496-509 RB denotes directionally
T5046 492-495 VBD denotes was
T5045 490-492 , denotes ,
T5044 489-490 -RRB- denotes ]
T5043 486-487 , denotes ,
T5042 484-486 CD denotes 24
T5041 487-489 CD denotes 25
T5040 483-484 -LRB- denotes [
T5039 467-474 JJ denotes genomic
T5038 461-466 NN denotes mouse
T5037 455-460 NN denotes 129Sv
T5036 449-454 NN denotes FIXII
T5035 442-448 NNP denotes Lambda
T5034 475-482 NN denotes library
T5033 440-441 DT denotes a
T5032 435-439 IN denotes from
T5031 426-434 VBN denotes obtained
T5030 415-425 RB denotes originally
T5029 413-415 , denotes ,
T5028 404-413 NNS denotes sequences
T5027 397-403 NN denotes intron
T5026 388-396 VBG denotes flanking
T5025 384-387 CC denotes and
T5024 381-383 CD denotes 10
T5023 377-380 CC denotes and
T5022 375-377 , denotes ,
T5021 374-375 CD denotes 9
T5020 372-374 , denotes ,
T5019 371-372 CD denotes 8
T5018 365-370 NNS denotes exons
T5017 356-364 VBG denotes spanning
T5016 339-346 JJ denotes genomic
T5015 333-338 NN denotes Acadm
T5014 329-330 HYPH denotes -
T5013 330-332 NN denotes kb
T5012 328-329 CD denotes 8
T5011 347-355 NN denotes fragment
T4974 149-155 VBN denotes filled
T4973 134-143 NNS denotes overhangs
T4972 130-133 DT denotes the
T4971 126-129 CC denotes and
T4970 120-125 NN denotes EcoRI
T4969 115-119 IN denotes with
T4968 102-105 VBD denotes was
T4967 106-114 VBN denotes digested
T4966 94-101 NN denotes plasmid
T4965 90-93 DT denotes The
T4964 89-175 sentence denotes The plasmid was digested with EcoRI and the overhangs were filled with Klenow enzyme.
T4963 88-89 . denotes .
T4962 87-88 -RRB- denotes )
T4961 86-87 SYM denotes +
T4960 85-86 -LRB- denotes (
T4959 77-85 NN denotes pGEM11Zf
T4958 74-76 IN denotes of
T5176 1102-1103 . denotes .
T5175 1091-1094 VBD denotes was
T5174 1081-1083 CD denotes 10
T4957 64-68 NN denotes SalI
T4956 69-73 NN denotes site
T4955 60-63 DT denotes the
T4954 55-59 IN denotes into
T4953 41-44 VBD denotes was
T4952 39-40 -RRB- denotes ]
T4951 37-39 CD denotes 23
T4950 36-37 -LRB- denotes [
T4949 45-54 VBN denotes subcloned
T4948 22-26 NN denotes gene
T4947 11-21 NN denotes resistance
T4946 2-10 NN denotes neomycin
T4945 27-35 NN denotes cassette
T4944 0-1 DT denotes A
T5010 325-327 DT denotes an
T5009 323-325 , denotes ,
T5008 510-516 VBN denotes cloned
T5007 319-323 RB denotes Next
T5006 318-561 sentence denotes Next, an 8-kb Acadm genomic fragment spanning exons 8, 9, and 10 and flanking intron sequences, originally obtained from a Lambda FIXII 129Sv mouse genomic library [24,25], was directionally cloned into the NotI and XhoI sites of pGEM11Zf(+).
T5005 317-318 . denotes .
T5004 308-309 -RRB- denotes )
T5003 307-308 SYM denotes +
T5002 306-307 -LRB- denotes (
T5001 298-306 NN denotes pGEMl1Zf
T5000 310-317 NN denotes plasmid
T4999 294-297 DT denotes the
T4998 291-293 IN denotes of
T4997 280-290 NN denotes polylinker
T4996 276-279 DT denotes the
T4995 269-275 IN denotes within
T4994 258-263 NN denotes EcoRI
T4993 264-268 NN denotes site
T4992 254-257 DT denotes the
T4991 244-253 VBD denotes destroyed
T4990 240-243 CC denotes and
T4989 233-239 NN denotes vector
T4988 229-232 DT denotes the
T4987 203-208 NN denotes blunt
T4986 209-213 NNS denotes ends
T4985 199-202 DT denotes the
T4984 196-198 IN denotes of
T4983 214-228 VBD denotes recircularized
T4982 187-195 NN denotes ligation
T4981 176-186 JJ denotes Subsequent
T4980 175-318 sentence denotes Subsequent ligation of the blunt ends recircularized the vector and destroyed the EcoRI site within the polylinker of the pGEMl1Zf(+) plasmid.
T4979 174-175 . denotes .
T4978 168-174 NN denotes enzyme
T4977 161-167 NN denotes Klenow
T4976 156-160 IN denotes with
T4975 144-148 VBD denotes were
R3117 T4944 T4945 det A,cassette
R3118 T4945 T4949 nsubjpass cassette,subcloned
R3119 T4946 T4947 compound neomycin,resistance
R3120 T4947 T4945 compound resistance,cassette
R3121 T4948 T4945 compound gene,cassette
R3122 T4950 T4951 punct [,23
R3123 T4951 T4945 parataxis 23,cassette
R3124 T4952 T4951 punct ],23
R3125 T4953 T4949 auxpass was,subcloned
R3126 T4954 T4949 prep into,subcloned
R3127 T4955 T4956 det the,site
R3128 T4956 T4954 pobj site,into
R3129 T4957 T4956 compound SalI,site
R3130 T4958 T4956 prep of,site
R3131 T4959 T4958 pobj pGEM11Zf,of
R3132 T4960 T4961 punct (,+
R3133 T4961 T4959 punct +,pGEM11Zf
R3134 T4962 T4961 punct ),+
R3135 T4963 T4949 punct .,subcloned
R3136 T4965 T4966 det The,plasmid
R3137 T4966 T4967 nsubjpass plasmid,digested
R3138 T4968 T4967 auxpass was,digested
R3139 T4969 T4967 prep with,digested
R3140 T4970 T4969 pobj EcoRI,with
R3141 T4971 T4967 cc and,digested
R3142 T4972 T4973 det the,overhangs
R3143 T4973 T4974 nsubjpass overhangs,filled
R3144 T4974 T4967 conj filled,digested
R3145 T4975 T4974 auxpass were,filled
R3146 T4976 T4974 prep with,filled
R3147 T4977 T4978 compound Klenow,enzyme
R3148 T4978 T4976 pobj enzyme,with
R3149 T4979 T4974 punct .,filled
R3150 T4981 T4982 amod Subsequent,ligation
R3151 T4982 T4983 nsubj ligation,recircularized
R3152 T4984 T4982 prep of,ligation
R3153 T4985 T4986 det the,ends
R3154 T4986 T4984 pobj ends,of
R3155 T4987 T4986 compound blunt,ends
R3156 T4988 T4989 det the,vector
R3157 T4989 T4983 dobj vector,recircularized
R3158 T4990 T4983 cc and,recircularized
R3159 T4991 T4983 conj destroyed,recircularized
R3160 T4992 T4993 det the,site
R3161 T4993 T4991 dobj site,destroyed
R3162 T4994 T4993 compound EcoRI,site
R3163 T4995 T4993 prep within,site
R3164 T4996 T4997 det the,polylinker
R3165 T4997 T4995 pobj polylinker,within
R3166 T4998 T4997 prep of,polylinker
R3167 T4999 T5000 det the,plasmid
R3168 T5000 T4998 pobj plasmid,of
R3169 T5001 T5000 nmod pGEMl1Zf,plasmid
R3170 T5002 T5003 punct (,+
R3171 T5003 T5000 punct +,plasmid
R3172 T5004 T5003 punct ),+
R3173 T5005 T4983 punct .,recircularized
R3174 T5007 T5008 advmod Next,cloned
R3175 T5009 T5008 punct ", ",cloned
R3176 T5010 T5011 det an,fragment
R3177 T5011 T5008 nsubjpass fragment,cloned
R3178 T5012 T5013 nummod 8,kb
R3179 T5013 T5011 nmod kb,fragment
R3180 T5014 T5013 punct -,kb
R3181 T5015 T5011 nmod Acadm,fragment
R3182 T5016 T5011 amod genomic,fragment
R3183 T5017 T5011 acl spanning,fragment
R3184 T5018 T5019 nmod exons,8
R3185 T5019 T5017 dobj 8,spanning
R3186 T5020 T5019 punct ", ",8
R3187 T5021 T5019 conj 9,8
R3188 T5022 T5021 punct ", ",9
R3189 T5023 T5021 cc and,9
R3190 T5024 T5021 conj 10,9
R3191 T5025 T5017 cc and,spanning
R3192 T5026 T5017 conj flanking,spanning
R3193 T5027 T5028 compound intron,sequences
R3194 T5028 T5026 dobj sequences,flanking
R3195 T5029 T5011 punct ", ",fragment
R3196 T5030 T5031 advmod originally,obtained
R3197 T5031 T5011 acl obtained,fragment
R3198 T5032 T5031 prep from,obtained
R3199 T5033 T5034 det a,library
R3200 T5034 T5032 pobj library,from
R3201 T5035 T5036 nmod Lambda,FIXII
R3202 T5036 T5034 nmod FIXII,library
R3203 T5037 T5034 nmod 129Sv,library
R3204 T5038 T5034 nmod mouse,library
R3205 T5039 T5034 amod genomic,library
R3206 T5040 T5041 punct [,25
R3207 T5041 T5034 parataxis 25,library
R3208 T5042 T5041 nummod 24,25
R3209 T5043 T5041 punct ",",25
R3210 T5044 T5041 punct ],25
R3211 T5045 T5008 punct ", ",cloned
R3212 T5046 T5008 auxpass was,cloned
R3213 T5047 T5008 advmod directionally,cloned
R3214 T5048 T5008 prep into,cloned
R3215 T5049 T5050 det the,sites
R3216 T5050 T5048 pobj sites,into
R3217 T5051 T5050 nmod NotI,sites
R3218 T5052 T5051 cc and,NotI
R3219 T5053 T5051 conj XhoI,NotI
R3220 T5054 T5050 prep of,sites
R3221 T5055 T5054 pobj pGEM11Zf,of
R3222 T5056 T5057 punct (,+
R3223 T5057 T5055 punct +,pGEM11Zf
R3224 T5058 T5057 punct ),+
R3225 T5059 T5008 punct .,cloned
R3226 T5061 T5062 det The,vector
R3227 T5062 T5063 nsubjpass vector,digested
R3228 T5064 T5063 auxpass was,digested
R3229 T5065 T5063 prep with,digested
R3230 T5066 T5065 pobj BamHI,with
R3231 T5067 T5066 cc and,BamHI
R3232 T5068 T5066 conj EcoRI,BamHI
R3233 T5069 T5070 aux to,remove
R3234 T5070 T5063 advcl remove,digested
R3235 T5071 T5072 det a,fragment
R3236 T5072 T5070 dobj fragment,remove
R3237 T5073 T5074 nummod 1.3,kb
R3238 T5074 T5072 nmod kb,fragment
R3239 T5075 T5074 punct -,kb
R3240 T5076 T5077 nmod BamHI,EcoRI
R3241 T5077 T5072 nmod EcoRI,fragment
R3242 T5078 T5077 punct /,EcoRI
R3243 T5079 T5072 amod genomic,fragment
R3244 T5080 T5072 acl containing,fragment
R3245 T5081 T5080 dobj exon,containing
R3246 T5082 T5081 nummod 10,exon
R3247 T5083 T5080 cc and,containing
R3248 T5084 T5080 conj flanking,containing
R3249 T5085 T5086 compound intron,sequences
R3250 T5086 T5084 dobj sequences,flanking
R3251 T5087 T5063 punct .,digested
R3252 T5089 T5090 det The,vector
R3253 T5090 T5092 nsubjpass vector,purified
R3254 T5091 T5090 amod digested,vector
R3255 T5093 T5090 punct ", ",vector
R3256 T5094 T5090 prep without,vector
R3257 T5095 T5096 det the,fragment
R3258 T5096 T5094 pobj fragment,without
R3259 T5097 T5098 nummod 1.3,kb
R3260 T5098 T5096 nmod kb,fragment
R3261 T5099 T5098 punct -,kb
R3262 T5100 T5101 nmod BamHI,EcoRI
R3263 T5101 T5096 nmod EcoRI,fragment
R3264 T5102 T5101 punct /,EcoRI
R3265 T5103 T5096 amod genomic,fragment
R3266 T5104 T5092 punct ", ",purified
R3267 T5105 T5092 auxpass was,purified
R3268 T5106 T5092 prep by,purified
R3269 T5107 T5108 compound gel,purification
R3270 T5108 T5106 pobj purification,by
R3271 T5109 T5092 cc and,purified
R3272 T5110 T5092 conj recircularized,purified
R3273 T5111 T5110 prep by,recircularized
R3274 T5112 T5111 pobj ligation,by
R3275 T5113 T5110 advcl using,recircularized
R3276 T5114 T5115 nummod three,oligonucleotides
R3277 T5115 T5113 dobj oligonucleotides,using
R3278 T5116 T5115 punct : ,oligonucleotides
R3279 T5117 T5118 nummod 5,AATTGTCGACA
R3280 T5118 T5115 appos AATTGTCGACA,oligonucleotides
R3281 T5119 T5117 punct ′,5
R3282 T5120 T5118 punct -,AATTGTCGACA
R3283 T5121 T5118 punct -,AATTGTCGACA
R3284 T5122 T5118 nummod 3,AATTGTCGACA
R3285 T5123 T5118 punct ′,AATTGTCGACA
R3286 T5124 T5118 punct ;,AATTGTCGACA
R3287 T5125 T5126 nummod 5,GATCGTCGACA
R3288 T5126 T5118 conj GATCGTCGACA,AATTGTCGACA
R3289 T5127 T5125 punct ′,5
R3290 T5128 T5126 punct -,GATCGTCGACA
R3291 T5129 T5126 nummod 3,GATCGTCGACA
R3292 T5130 T5126 punct ′,GATCGTCGACA
R3293 T5131 T5126 punct ;,GATCGTCGACA
R3294 T5132 T5126 cc and,GATCGTCGACA
R3295 T5133 T5134 nummod 5,TCGATGTCGAC
R3296 T5134 T5126 conj TCGATGTCGAC,GATCGTCGACA
R3297 T5135 T5133 punct ′,5
R3298 T5136 T5134 punct -,TCGATGTCGAC
R3299 T5137 T5134 punct -,TCGATGTCGAC
R3300 T5138 T5134 nummod 3,TCGATGTCGAC
R3301 T5139 T5134 punct ′,TCGATGTCGAC
R3302 T5140 T5092 punct .,purified
R3303 T5142 T5143 det The,vector
R3304 T5143 T5145 nsubj vector,contained
R3305 T5144 T5143 amod recircularized,vector
R3306 T5146 T5143 punct ", ",vector
R3307 T5147 T5143 acl resulting,vector
R3308 T5148 T5147 prep from,resulting
R3309 T5149 T5150 det the,ligation
R3310 T5150 T5148 pobj ligation,from
R3311 T5151 T5150 prep of,ligation
R3312 T5152 T5153 det the,arm
R3313 T5153 T5151 pobj arm,of
R3314 T5154 T5153 amod long,arm
R3315 T5155 T5150 prep to,ligation
R3316 T5156 T5157 det the,arm
R3317 T5157 T5155 pobj arm,to
R3318 T5158 T5157 amod short,arm
R3319 T5159 T5150 prep of,ligation
R3320 T5160 T5159 pobj homology,of
R3321 T5161 T5145 punct ", ",contained
R3322 T5162 T5163 det a,site
R3323 T5163 T5145 dobj site,contained
R3324 T5164 T5163 amod unique,site
R3325 T5165 T5163 compound SalI,site
R3326 T5166 T5167 advmod where,deleted
R3327 T5167 T5163 relcl deleted,site
R3328 T5168 T5169 det the,region
R3329 T5169 T5167 nsubjpass region,deleted
R3330 T5170 T5171 nummod 1.3,kb
R3331 T5171 T5169 nmod kb,region
R3332 T5172 T5171 punct -,kb
R3333 T5173 T5169 nmod exon,region
R3334 T5174 T5173 nummod 10,exon
R3335 T5175 T5167 auxpass was,deleted
R3336 T5176 T5145 punct .,contained

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T5203 1076-1080 SO:0000147 denotes exon
T5202 975-983 GO:0006266 denotes ligation
T5201 948-954 SO:0000440 denotes vector
T5200 830-838 GO:0006266 denotes ligation
T5199 757-764 SO:0001026 denotes genomic
T5198 745-750 PR:P23940 denotes BamHI
T5197 718-724 SO:0000440 denotes vector
T5196 687-693 SO:0000188 denotes intron
T5195 678-686 SO:0000357 denotes flanking
T5194 666-670 SO:0000147 denotes exon
T5193 638-645 SO:0001026 denotes genomic
T5192 626-631 PR:P23940 denotes BamHI
T5191 591-596 PR:P23940 denotes BamHI
T5190 566-572 SO:0000440 denotes vector
T5189 467-474 SO:0001026 denotes genomic
T5188 461-466 NCBITaxon:10088 denotes mouse
T5187 397-403 SO:0000188 denotes intron
T5186 388-396 SO:0000357 denotes flanking
T5185 365-370 SO:0000147 denotes exons
T5184 339-346 SO:0001026 denotes genomic
T5183 310-317 SO:0000155 denotes plasmid
T5182 233-239 SO:0000440 denotes vector
T5181 187-195 GO:0006266 denotes ligation
T5180 94-101 SO:0000155 denotes plasmid
T5179 22-35 SO:0005853 denotes gene cassette
T5178 2-10 CHEBI:7507 denotes neomycin

2_test

Id Subject Object Predicate Lexical cue
16121256-1997203-86078344 37-39 1997203 denotes 23
16121256-7829081-86078345 484-486 7829081 denotes 24
16121256-8666240-86078346 487-489 8666240 denotes 25
T4499 37-39 1997203 denotes 23
T57310 484-486 7829081 denotes 24
T81312 487-489 8666240 denotes 25

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T5245 1095-1102 SO_EXT:sequence_deletion_process denotes deleted
T5244 1076-1080 SO_EXT:0000147 denotes exon
T5243 1074-1075 CHEBI_SO_EXT:base denotes b
T5241 948-954 SO_EXT:0000440 denotes vector
T5240 935-947 SO_EXT:sequence_circularization denotes circularized
T5239 851-867 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T5238 830-838 GO:0006266 denotes ligation
T5237 814-826 SO_EXT:sequence_circularization denotes circularized
T5236 757-764 SO_EXT:0001026 denotes genomic
T5235 745-750 PR_EXT:P23940 denotes BamHI
T5234 743-744 CHEBI_SO_EXT:base denotes b
T5233 718-724 SO_EXT:0000440 denotes vector
T5232 694-703 SO_EXT:biological_sequence denotes sequences
T5231 687-693 SO_EXT:0000188 denotes intron
T5230 678-686 SO:0000357 denotes flanking
T5229 666-670 SO_EXT:0000147 denotes exon
T5228 638-645 SO_EXT:0001026 denotes genomic
T5227 626-631 PR_EXT:P23940 denotes BamHI
T5226 624-625 CHEBI_SO_EXT:base denotes b
T5225 591-596 PR_EXT:P23940 denotes BamHI
T5224 566-572 SO_EXT:0000440 denotes vector
T5223 510-516 SO_EXT:sequence_cloning_process denotes cloned
T5222 467-474 SO_EXT:0001026 denotes genomic
T5221 461-466 NCBITaxon:10088 denotes mouse
T5220 404-413 SO_EXT:biological_sequence denotes sequences
T5219 397-403 SO_EXT:0000188 denotes intron
T5218 388-396 SO:0000357 denotes flanking
T5217 365-370 SO_EXT:0000147 denotes exons
T5216 339-346 SO_EXT:0001026 denotes genomic
T5215 333-338 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T5214 331-332 CHEBI_SO_EXT:base denotes b
T5213 310-317 SO_EXT:0000155 denotes plasmid
T5212 233-239 SO_EXT:0000440 denotes vector
T5211 216-228 SO_EXT:sequence_circularization denotes circularized
T5210 187-195 GO:0006266 denotes ligation
T5209 168-174 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T5208 94-101 SO_EXT:0000155 denotes plasmid
T5207 22-35 SO_EXT:0005853 denotes gene cassette
T5206 2-10 CHEBI:7507 denotes neomycin
T5242 975-983 GO:0006266 denotes ligation